LOCUS       DACEXQ010000001        60159 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.001, whole genome shotgun sequence.
ACCESSION   DACEXQ010000001 DACEXQ010000000
VERSION     DACEXQ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60159
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.001"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..350
                     /locus_tag="IXH04_000001"
     CDS             <1..350
                     /locus_tag="IXH04_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="signal peptidase"
                     /protein_id="HAQ1850648.1"
                     /translation="PKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADT
                     KTYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFV
                     ELKKKRRRTQKFL"
     gene            478..1161
                     /locus_tag="IXH04_000002"
     CDS             478..1161
                     /locus_tag="IXH04_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAQ1850649.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1470..1543
                     /locus_tag="IXH04_000003"
     tRNA            1470..1543
                     /locus_tag="IXH04_000003"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1504..1506,aa:Arg,seq:tct)
     gene            1702..2061
                     /locus_tag="IXH04_000004"
     CDS             1702..2061
                     /locus_tag="IXH04_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAQ1850650.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2184..3815
                     /locus_tag="IXH04_000005"
     CDS             2184..3815
                     /locus_tag="IXH04_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAQ1850651.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(3877..4518)
                     /locus_tag="IXH04_000006"
     CDS             complement(3877..4518)
                     /locus_tag="IXH04_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ1850652.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            4800..5108
                     /gene="rplU"
                     /locus_tag="IXH04_000007"
     CDS             4800..5108
                     /gene="rplU"
                     /locus_tag="IXH04_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAQ1850653.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5135..5479
                     /locus_tag="IXH04_000008"
     CDS             5135..5479
                     /locus_tag="IXH04_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAQ1850654.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTEEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5492..5785
                     /gene="rpmA"
                     /locus_tag="IXH04_000009"
     CDS             5492..5785
                     /gene="rpmA"
                     /locus_tag="IXH04_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAQ1850655.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(5976..7193)
                     /locus_tag="IXH04_000010"
     CDS             complement(5976..7193)
                     /locus_tag="IXH04_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ1850656.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEESTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEGSPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKKDKVATVACMNKLLKCMHAMVRAH
                     TEYDYAYAVSVDH"
     gene            7804..10638
                     /locus_tag="IXH04_000011"
     CDS             7804..10638
                     /locus_tag="IXH04_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1850657.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            10829..11320
                     /locus_tag="IXH04_000012"
     CDS             10829..11320
                     /locus_tag="IXH04_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAQ1850658.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            11423..12412
                     /gene="manX"
                     /locus_tag="IXH04_000013"
     CDS             11423..12412
                     /gene="manX"
                     /locus_tag="IXH04_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAQ1850659.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            12445..13248
                     /locus_tag="IXH04_000014"
     CDS             12445..13248
                     /locus_tag="IXH04_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAQ1850660.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            13269..14183
                     /locus_tag="IXH04_000015"
     CDS             13269..14183
                     /locus_tag="IXH04_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAQ1850661.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            14266..14640
                     /locus_tag="IXH04_000016"
     CDS             14266..14640
                     /locus_tag="IXH04_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAQ1850662.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            14779..15024
                     /locus_tag="IXH04_000017"
     CDS             14779..15024
                     /locus_tag="IXH04_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ1850663.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            15196..16260
                     /locus_tag="IXH04_000018"
     CDS             15196..16260
                     /locus_tag="IXH04_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ1850664.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            16506..16931
                     /locus_tag="IXH04_000019"
     CDS             16506..16931
                     /locus_tag="IXH04_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ1850665.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            16924..17373
                     /gene="nusB"
                     /locus_tag="IXH04_000020"
     CDS             16924..17373
                     /gene="nusB"
                     /locus_tag="IXH04_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAQ1850666.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            17470..18324
                     /locus_tag="IXH04_000021"
     CDS             17470..18324
                     /locus_tag="IXH04_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAQ1850667.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            18325..19662
                     /locus_tag="IXH04_000022"
     CDS             18325..19662
                     /locus_tag="IXH04_000022"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAQ1850668.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            19667..19891
                     /locus_tag="IXH04_000023"
     CDS             19667..19891
                     /locus_tag="IXH04_000023"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAQ1850669.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            19893..20783
                     /locus_tag="IXH04_000024"
     CDS             19893..20783
                     /locus_tag="IXH04_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ1850670.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            20792..21610
                     /locus_tag="IXH04_000025"
     CDS             20792..21610
                     /locus_tag="IXH04_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAQ1850671.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            21717..22166
                     /locus_tag="IXH04_000026"
     CDS             21717..22166
                     /locus_tag="IXH04_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ1850672.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            22184..23866
                     /gene="recN"
                     /locus_tag="IXH04_000027"
     CDS             22184..23866
                     /gene="recN"
                     /locus_tag="IXH04_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAQ1850673.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(23921..24037)
                     /locus_tag="IXH04_000028"
     CDS             complement(23921..24037)
                     /locus_tag="IXH04_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAQ1850674.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(24110..25066)
                     /locus_tag="IXH04_000029"
     CDS             complement(24110..25066)
                     /locus_tag="IXH04_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ1850675.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            25237..25605
                     /locus_tag="IXH04_000030"
     CDS             25237..25605
                     /locus_tag="IXH04_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAQ1850676.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            25775..26206
                     /gene="mraZ"
                     /locus_tag="IXH04_000031"
     CDS             25775..26206
                     /gene="mraZ"
                     /locus_tag="IXH04_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAQ1850677.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            26227..27186
                     /gene="rsmH"
                     /locus_tag="IXH04_000032"
     CDS             26227..27186
                     /gene="rsmH"
                     /locus_tag="IXH04_000032"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAQ1850678.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            27191..27592
                     /gene="ftsL"
                     /locus_tag="IXH04_000033"
     CDS             27191..27592
                     /gene="ftsL"
                     /locus_tag="IXH04_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAQ1850679.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            27593..29785
                     /locus_tag="IXH04_000034"
     CDS             27593..29785
                     /locus_tag="IXH04_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ1850680.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSVYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            29811..30773
                     /locus_tag="IXH04_000035"
     CDS             29811..30773
                     /locus_tag="IXH04_000035"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAQ1850681.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            30787..32148
                     /locus_tag="IXH04_000036"
     CDS             30787..32148
                     /locus_tag="IXH04_000036"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAQ1850682.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            32174..33262
                     /gene="murG"
                     /locus_tag="IXH04_000037"
     CDS             32174..33262
                     /gene="murG"
                     /locus_tag="IXH04_000037"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAQ1850683.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            33277..34497
                     /locus_tag="IXH04_000038"
     CDS             33277..34497
                     /locus_tag="IXH04_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAQ1850684.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            34653..35978
                     /gene="ftsA"
                     /locus_tag="IXH04_000039"
     CDS             34653..35978
                     /gene="ftsA"
                     /locus_tag="IXH04_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAQ1850685.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNIKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            36001..37242
                     /gene="ftsZ"
                     /locus_tag="IXH04_000040"
     CDS             36001..37242
                     /gene="ftsZ"
                     /locus_tag="IXH04_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAQ1850686.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            37259..37936
                     /locus_tag="IXH04_000041"
     CDS             37259..37936
                     /locus_tag="IXH04_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAQ1850687.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            37950..38549
                     /locus_tag="IXH04_000042"
                     /pseudo
     CDS             37950..38549
                     /locus_tag="IXH04_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
     gene            38564..38851
                     /locus_tag="IXH04_000043"
     CDS             38564..38851
                     /locus_tag="IXH04_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAQ1850688.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            38900..39682
                     /locus_tag="IXH04_000044"
     CDS             38900..39682
                     /locus_tag="IXH04_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAQ1850689.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            39703..40410
                     /locus_tag="IXH04_000045"
     CDS             39703..40410
                     /locus_tag="IXH04_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAQ1850690.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            40672..43470
                     /gene="ileS"
                     /locus_tag="IXH04_000046"
     CDS             40672..43470
                     /gene="ileS"
                     /locus_tag="IXH04_000046"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAQ1850691.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKSYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(43519..45045)
                     /locus_tag="IXH04_000047"
     CDS             complement(43519..45045)
                     /locus_tag="IXH04_000047"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAQ1850692.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(45060..45707)
                     /locus_tag="IXH04_000048"
     CDS             complement(45060..45707)
                     /locus_tag="IXH04_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAQ1850693.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(45891..46220)
                     /locus_tag="IXH04_000049"
     CDS             complement(45891..46220)
                     /locus_tag="IXH04_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAQ1850694.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            46397..47125
                     /gene="sfsA"
                     /locus_tag="IXH04_000050"
     CDS             46397..47125
                     /gene="sfsA"
                     /locus_tag="IXH04_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAQ1850695.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            47141..48154
                     /locus_tag="IXH04_000051"
     CDS             47141..48154
                     /locus_tag="IXH04_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1850696.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            48154..49431
                     /locus_tag="IXH04_000052"
     CDS             48154..49431
                     /locus_tag="IXH04_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ1850697.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            49494..52196
                     /locus_tag="IXH04_000053"
     CDS             49494..52196
                     /locus_tag="IXH04_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1850698.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGASQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            52348..52665
                     /locus_tag="IXH04_000054"
     CDS             52348..52665
                     /locus_tag="IXH04_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1850699.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            52695..53015
                     /locus_tag="IXH04_000055"
     CDS             52695..53015
                     /locus_tag="IXH04_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ1850700.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            53123..54583
                     /locus_tag="IXH04_000056"
     CDS             53123..54583
                     /locus_tag="IXH04_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1850701.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            54651..54872
                     /locus_tag="IXH04_000057"
     CDS             54651..54872
                     /locus_tag="IXH04_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1850702.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            54903..55085
                     /locus_tag="IXH04_000058"
     CDS             54903..55085
                     /locus_tag="IXH04_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAQ1850703.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            55085..55498
                     /locus_tag="IXH04_000059"
     CDS             55085..55498
                     /locus_tag="IXH04_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAQ1850704.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            55621..56802
                     /locus_tag="IXH04_000060"
     CDS             55621..56802
                     /locus_tag="IXH04_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1850705.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            57191..57264
                     /locus_tag="IXH04_000061"
     tRNA            57191..57264
                     /locus_tag="IXH04_000061"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:57225..57227,aa:Arg,seq:cct)
     gene            complement(57333..58472)
                     /locus_tag="IXH04_000062"
     CDS             complement(57333..58472)
                     /locus_tag="IXH04_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1850706.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(58771..59406)
                     /locus_tag="IXH04_000063"
     CDS             complement(58771..59406)
                     /locus_tag="IXH04_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1850707.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(59519..60154)
                     /locus_tag="IXH04_000064"
     CDS             complement(59519..60154)
                     /locus_tag="IXH04_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HAQ1850708.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
BASE COUNT        19672 a        10195 c        12803 g        17489 t
ORIGIN      
        1 gaccgaaaaa tacaaatgag attttagtcg gagatattat tacatattat gaaaattcag
       61 gaaaaaaaat ctcgacgcgt cgcgtaatag ccgttgaaaa caaccacgaa gatttttata
      121 caaaagctga tacaaagact tatattgagc ctggagtagt aaaaaagaga aatattatcg
      181 gtagtcccat ctttcagatt ccatatattg gactagtcct ttcaaaaacg gcatttgcat
      241 ggattcaggg gttattcttt acgattgctt tttgcctaac aggaatcaca gcatggaata
      301 cctttgtaga gttgaaaaag aagcggagac gaacacagaa atttttgtaa gaatgttaaa
      361 aacataaatg tattccagag atccaacgac tcttctgaac atgaaacacg agaaaacact
      421 catgcagctt gtagatttag tcagttgttc gtataatgtg tgaagaggag gagtagtatg
      481 cgcaatgaaa tgatgcatcg tcctgtagtc aaagaagaac tattggattt tatgcgtacc
      541 cagcaaaaaa aattaccggg tgagctaggc aaactggaag aagaagctca tgtagcagaa
      601 gtaccagtta ttccccatga aacagttgta tttttgaaat ttttacttgg acagcttcag
      661 cctgaacgaa tcttggagat cggagcagct atcggttttt catcaagtgt catggccacg
      721 acaatcggag aaaacgctca tgttacgaca attgatcgtt tcgatgtcat gatcgagaaa
      781 gctaaaaaaa actatgaacg tctaggactg acagataaag tgactttgct tgaaggtcaa
      841 gcagcagata tcttgccgga gttgtctggt ccgtatgact ttatttttat ggatagtgca
      901 aaatcaaaat atattgaatt tttacctgaa tgcttacgtt tgttaagaaa aggtggcgtt
      961 ttgatggtag atgacatctt ccaaggagga acgatcttgc tcccagatga agagattccc
     1021 cgtggcaaac gagctatcca tcgtaaatta aatgaatttc ttcgtgttgt gatggatcat
     1081 cccgatttga catcgacaat cttgccatta ggtgatggtg tgatcttaat gacaaaggaa
     1141 tcagaaacaa tcgagttgtg atacatgatt atggcagcct gtagataatc gattgaatcg
     1201 agggctcaat tcgaacttga tgtaaaagtt tctatatttg taagtcaatt aaaggataca
     1261 ggaaatctaa ataaacagga gtagcatgaa gtcaatgtag ctcctgtttt caattgataa
     1321 ccttagaaca gctgaaggag aatgtctcgt tttaaaagat tgatttagta gttgggccaa
     1381 aataattcat tatttgctaa ttaaataact atttcgtatt gacagaagca atttaattat
     1441 ctataattat tgatgttccg ctgattcttg tcatagtagc tcagctggat agagcatccg
     1501 ccttctaagc ggacggtcgg gggttcgaat ccctcctgtg acgctaaaaa accgttagaa
     1561 actgaggttt ctaacggttt ttctttgttt tttgtgccaa tattgtttgt tttcaaaggc
     1621 aaataaaata aaaaaacatc cttatcgagt acaattataa ggagcaaaaa aataaaactc
     1681 atcagaaaag aggaagaaga tatgacgata ttatctagaa ttttagtgac tctagttgca
     1741 attgaatttt tttatatcat gtatatcgaa acggtgagaa ctgattctga cacgacaagt
     1801 agggttttta aaatgagtaa agaagagtta agcagaaaat ctgttcaaac attgtttaag
     1861 aaccaaggag tgtacaatgg actattagga gtgggactac tttatggagc ttatctttct
     1921 tccgcatcta aagaaatcac aagtatgcta ttaatatcta ttttttttgt tgcattatat
     1981 ggcagtttga ctagcgatcg ttggatttta ctcaaacaag gtgggctagc tctgttggca
     2041 ttgctttcta ttttcttata aatatagaaa aaataagaat ttatctaaca ggaaactttg
     2101 tgaaaaatat tactttatct catgaaatat gatattctaa tgaatggtat tttagaaaat
     2161 ttcatgaaaa aggagttaga taggtgttca aaaattatgt ttcctcacta aaaaaagaat
     2221 tttcaggtta tgatctttca agatttcaga aagatctttt ggctgggatc acagtagctg
     2281 ctgtagcatt gcctttagcc ttggcttttg gggtatcaag cggagctgat gcggccgcag
     2341 gtttgattac ggctatcatt gccggtatcg ttattggcgg tttatcaggc ggtttttatc
     2401 aaatatcagg tccgacaggt gcgatggcag ctatcttgat gtctattgcg gcaacaaagg
     2461 gaatgcaagg tgtattgctt gctacttttt tagcgggtct atttttactt gcagcaggac
     2521 ttttccgatt aggaacactg acttctttta tcccagcacc cgtaattacg gggtttactt
     2581 caggtattgc tatcattatt gcattaggtc agatcgataa tttatttggt gttcactcgg
     2641 aaggtacaaa cgttattgaa aaactaagta gttatcaaac gataggattt ccgataaaca
     2701 cagcctcttt gatcatggga gggctagtca ttttagggat gtttatctat ccaaaaaaat
     2761 gggcgaaacg aatgcctagt tcgctgctgg ctatcatttt ggcaacagca gttatgctat
     2821 tgttccattt accagtagca actgtgggta agattccaca aacattgatc agtagtaatc
     2881 gtttgaatat gggagatttt agcttttctg ccttgcaaca agtggcagtt ccagctatta
     2941 gcattgcttt attaggaatg attgaaagct tgctatgtgg tgcttcggct ggccgaatgg
     3001 caaataagcc cttagatagc aatcaagaat taatggcgca aggaattgga aatctgttgc
     3061 ttcctttctt tggcgggatt cctgctactg cggccatcgc ccgtacaagc gtggccatca
     3121 aatctggtgc acaaactaga ttagctggta tgatacatgc agttgtctta ctgctctcca
     3181 tgcttgtttt tgcgccagtg atgtcacaga ttccaatgcc agcacttgca ggtgttttga
     3241 ttgtgacggc ttggcgtatg aatgaatggg aaacgatcaa ggagctattt actaataaat
     3301 attggtcagc agttctattg tttatcctta cgatgatttg tacagttatc tttgatttaa
     3361 gtattgctat cgtgatcggg gtgatcggcg gctgtgtatt ttttgttgtc aaaagcgctg
     3421 ctatcacgat ttcattagaa acgatcgatt ggcaaagaat ggggctgcca gaaacggaaa
     3481 agctgaataa ctggacagta gtgtatatta gtggtccact gttttttatg tcttcagaaa
     3541 aactcaaaaa taaattggaa gaactaaatc atcacgatgg tgtcattttc tccatgcgag
     3601 gcgtaccaag tattgatttg acggcacgtg cagtttttga tgaatttcaa gaaaaagcag
     3661 agcaaagagg acaaacgatt atctatactt ccttgcagcc agaagtagaa aaacaacttt
     3721 ctcacttgtg ggaacataca ttcacgcatc aacatcaaac agttgctcat gcgttgacag
     3781 ctttacatgc gaaatatact gcaattagcc attaaaacaa tgaaaaagaa cagacaagac
     3841 tgtaaactga agattttatg gtcttgtctg ttctttttaa tattgctgta aaagtgaagc
     3901 tagtacttgc cagtcttgat ggataacagg tacaagggag cgattgtaga aaatagctgc
     3961 tggatgaaaa agtggaagaa ttcgatagtt tttttctgac cattcgtagg catccttttg
     4021 ttctgttaat tgtaagacag gccctgtata gagttgacca tgatttttgg taaccgtata
     4081 ttctttgcca agtaaacgtt gaagaccaat gtttccaagt gtcgcaatta attttggctg
     4141 gaccttcatc agttcgtaat ccaatattgg tgcatgcgca aatacttcgg aacgagtagg
     4201 tgtccgattt ggataaacag tttctgactg ttgtgtccgt ttattgatcc gatgagtcac
     4261 gcgatacggg cggctcctta cagcactggt gatatacacc tcttctcgtg ttaagccagt
     4321 tgaagaaagt gcctgcatca attcttttcc agcttgaccg ctaaaaggaa catgcgtatc
     4381 aatctctgtt ttccctgggg cttctcccac gagcatcagt ttaggatgaa gaggtccttg
     4441 cccagcgata aatccttcta caggacggcc gttcattctt aaagaagctt cttgtatcag
     4501 ttcttttggg tattccattt atgctcctcc tttttctttt attataagaa taaaagaaac
     4561 acaatgcgac tattttattt ggtagaactg tgaggataga aaagcagaag tattgcttga
     4621 atttttgttt tactttttga ttttaaattt agttgactaa cggttgctgt tctggtaagc
     4681 tagtactgtt gtaattgtgg actccacagc tacaaccgct cagaacaagt aataagcaca
     4741 gcatatgctg tcacttcgga tggcgagtct aagtatatca gaaggaggtg ctttatagca
     4801 tgtacgcaat tattaaaact ggtggtaaac aagtaaaagt tgaagtggga caagcaatct
     4861 atgttgaaaa acttgacgtt gaagcaggag aaaaagttgt ttttgacgaa gttatcttag
     4921 taggcggaga atctacaaaa gtaggcgctc caactgttgc aggtgcaact gttgaaggta
     4981 ctgttgaaaa acacggcaaa caaaagaaag tcgttacttt caaatacaaa cctaaaaaac
     5041 attcacaccg taaacaaggt catcgtcaac catatacaaa agtggttatc gacgcgatca
     5101 acgcttaata gcgtttgaga aagaaggcct atatatgatc aaaggaacat ttaagcgaaa
     5161 tgacgcaggt cagatcgttt cattcacttt gaccgggcat gctgatgctg gtccttatgg
     5221 aagtgatatc gtttgtgcgg gtgtttctgc gttagcgatc agcactgtca acgggattgc
     5281 ctcattagcc ggttttgagc cgattgttga aatgaatgaa gaagaaggcg gttatcttta
     5341 taccgaagtc acttcaggaa tgacagaaga acaaaacaat attgctgaga ttctattaga
     5401 aaatctttta ttaggattac aatcgataga agctgaaaac tcagaatata tccaaattaa
     5461 aacaattaat gataaatagg aggtgcagac catgttgtta tctatgaatc tacaattatt
     5521 tgcccacaaa aaaggtggcg gttctacatc taacggccgt gactcagaat caaaacgtct
     5581 aggtgctaaa cgtgctgatg gacaaacagt tacaggtgga tcaattcttt accgtcaacg
     5641 cggtacaaaa atctatccag gtgtaaacgt gggtatcggt ggagacgaca ctttatttgc
     5701 aaaagttgat ggcgtagtac gtttcgaacg taaaggccgt aacaaaaaac aagtgtctgt
     5761 ttatccagta gctcaagaag cttaattgaa tcttatgatt aaagaggttg tgacaagact
     5821 tttgtcacag cctttttcct acgattagtc aagtaaaagg tgcaagaaaa atagctcgta
     5881 aacgaaagcg acaaaatcaa agcacaccaa ataaagcccc gttcgtaaaa aattttcgat
     5941 ctgggcaaag aagtggtata ctgtaagtgg tttagttaat ggtccactga gaccgcgtac
     6001 gcatagtcgt actctgtatg tgccctaacc atagcgtgca tacatttcag aagcttgttc
     6061 atacaagcaa ctgtggcaac cttatctttc ttagggatag gttgcttttt taatttgtag
     6121 taataatcga caatatgatt aggagctgcg cgttgctggc gaatcatatt tcgaatggtg
     6181 aagaaaataa ttttccgtgc tttgggatta ccacgcttat tgatgcggtc ttggccagta
     6241 tacttacctg aatgataccg tcggatgtcg attccgacaa aggcattcaa ttgtttatgg
     6301 gttttaaatc gtgtgatatc tcctaattca ccgagcaata aagcggctgt gagtgcgcca
     6361 atcccaggga acgtttggta caacaaaaag caaggagagc cttcggcttt tttgatcatt
     6421 tgtgtcgcca gtgctttttt ttgttcgagt aaatcttgga gaagttcggc gtaatagatc
     6481 gttttttgac agtggatact gtctttttca acagctggat aggcacactg tgcatagtgg
     6541 atgatttgat ctgccttttg ctcggcacga ttcgcggaga tttttttccg tgtttcgttg
     6601 atcagtttat tttttatttt tgtccgagta gatgccaata caaaatctgg atggggaaaa
     6661 agacggatca atgtcaatgc gtaaggggtc agcctgtttg aaaagaactg ttcaagctct
     6721 ggaaaagtca attgaaggca gttgtgtaaa tccatgcgca gacgagtgat tttcttctca
     6781 atttcttgat aaaaacgggc taaatcgcgc atctctaagt aaaaattgct ttgtacttct
     6841 tttggctgac gttgattttt aagatgagtc tgtgcgagcc ggtgggcatc cgatttatct
     6901 gttttccaac tgcgtaaggt gctttcttcc atctgttttt tcgcctctaa cgggtttaac
     6961 aaacagtaag aaaagtgatg atcttgaaag aacttttcga ctggtctgga ataaatcccc
     7021 gttgcttcaa aaacaatcaa tggacgctca ggcaagtgct ggatctcttc gagtaatgca
     7081 tgaaatcctc tttgagtgtg ttcaatctcg tcttcaaata agcaggtgtc gtctttgtat
     7141 aggacactgt agctgtgtcc cattgaaaca tctaatgcaa gaatgtaagg catttgtttt
     7201 cctccttttt aagttttgaa agaactttac tgcttcattt cgattccaat ttcctataca
     7261 cgggctcatg gccccaacat acgtaaacct gattctaaaa tgacagtaaa gaagttcggt
     7321 ttttgatagg gactcgtagt cctcaaggct ttgttcgaac tttctttcac tcttcactat
     7381 aatctaaaaa tagaaaatag aggagaacat cattcgtcaa tgatgttccc ctctaatctt
     7441 agtatgtttt gtttataaaa ataaaagttg tagtggaggc tacgaagttt actttgtctt
     7501 tttacactta atattttttt gccaatttgg aatcaggatg ttttgataac tatcgttttc
     7561 aattgatgga agctatattg tttgctatca gtgaaataat tatatggtaa acagtgttct
     7621 ttttcttgtt tccattataa aaatcgtatc gggatttttc gttcttttaa attgaacatt
     7681 tctatctatc cgcgtgttat caacaaaaca aaatcaggta aatagttgac gaaacagaaa
     7741 taaatacata gtattatgtt ttgaagccta agtacaatag tctatagaaa aggagcgata
     7801 ccattggcaa aacgcattga ccgaatttat atgtatgtca aagaaaaaac cgctcattta
     7861 acaaaggcag aatatgatca aggagtaaca acacaggaaa ttgctgaagt gttagggatt
     7921 cagcgaacaa atagcagtaa agatctgaat cagctagtgc gcgaaggaaa actgataaag
     7981 acagacggac gaccagtacg ttacatatat caaacactcg ttacgcacgt aaagccagtt
     8041 acaaaacatg tagtcagtta caaagaaacg ccggtctctg aagaaaaaaa tacactccaa
     8101 atagatacta aggatatctt tgcaaaaatc attggagcta acggcagcat gaaaaattca
     8161 gtggaacagg caaaagctgc gattttgtat ccgccaagag gattaaattg tttgattaca
     8221 ggacctaccg gatcaggaaa gacttatttt gcacacgcga tgtatcattt tgcaaaagcg
     8281 catcatgtaa tagatgagga aacggaactg attgttttca attgtgctga ttatgccaat
     8341 aaccctgagc ttttgatgag tcatttattt gggtacgcaa aaggtgcttt tacaggcgca
     8401 gatgaagaaa aaatggggat tatcgatcaa gcggatggcg gtatgctgtt tttggatgag
     8461 attcaccggt tgccgcctga aggtcaagaa atgatctttt actttatgga tcatggaact
     8521 tacagtcgtc ttggggaaac aacgaaatcc catgaagcaa atgtacggat cgtaggtgcg
     8581 accacagaag acccaggttc ttctctatta gaaacttttg ttcggcgtat cccaatcaat
     8641 atcaaacttc cggcttttga aaaacgtcct gcaaatgaga aagtcgattt agtaaaaatc
     8701 atgattgccc acgaagcaaa ccgcacgcag cgaaagattt ctttgacaga agatgtagca
     8761 aaagcattga tcggcagtgt gacctatgga aatataggac aattgaagtc aaatatccag
     8821 ttagtatgtg cacgaggatt tctaaatcat atgcaatcac ccgaaatctc gatcacaatc
     8881 gatgatttga cagaagggat tcgtagcgga ttgattcaat tagcaagcaa ccgcagtgca
     8941 atggcagagc tttctaaaat attggagcca aagataactg tagcaccaaa tgatacgatc
     9001 atgaaaatcc aatcggactc ctatgagtta ccttataatc tatatgatat catcggagat
     9061 aaagcagctt tactgaagtc ggatggtttg gatcaagaag caatcaatca tttcatttct
     9121 acagatatca acatccatct gaagtctttc tataaagacc atggtttttc ttttaatgca
     9181 gacagcaaat tagcagaatt tgtagatccg aaaatcatag aagtgaccaa tcagatttat
     9241 gtaatggtca aaagatcttt accatatgag tttcagcaaa actttattta tgctatgagc
     9301 ttgcatatca gttctttttt gaaaaaaatc aacttaggag aagaacggca tacgaatgat
     9361 aacatccgtg agatggcttt tgattttcca gatgaatatg ctgttgcgaa agaagtgcgt
     9421 agttatatcg aaacgtattt tcaagtggag attccagaaa gtgaagacta ttacttgacc
     9481 gttctactcg tctcgttacg ggcaaatcaa gcaagcggca gaatcggtgt agtcgttgca
     9541 gctcacggaa acagcacagc aagcagtatg gtccaagttg tccaacagtt gttagatgca
     9601 gagaacgtac gagcagtaga catgcctttg gatatggatc cgaaaacagc attggaccga
     9661 attgaacgca atgtacaaga agtagatgaa ggaagcgggg tcatactctt agtagatatg
     9721 gggtcccttg cgtcgtttaa ttcgcaaatt caaagagaca ctggaattcc agtacgtact
     9781 gttgatatgg taacaacttc tctagttttg gaaactgtac gaaaggtctc agtattaggg
     9841 acagatctag atatgttgca tgaatcattg aaaaattttc gaggttacgc agaaatatct
     9901 tctgtagaat ctgttcagga agcaaaacct attcataaga aaaaagctgt tttggctatc
     9961 tgcgcatctg gcgaaggtac agcacaaaga atcaaagaat tgatcgaacg agcggtaaac
    10021 aagcgtcaag aaacagaatt agaagttcta gctttgtcga ttgtggaagt gaaagaagaa
    10081 ctgccagtta tccaaaaaga atatcagatc atagctacta ctgggattac tgatttgaat
    10141 atccaagccc catttattcc tttagaacgt tttattgatc aaaatatcga agtcattttg
    10201 gatcagttgc tgatggaatc agaattggag gaaactgaat tcatctccct agatgaagag
    10261 tcagctaaaa atacgtgtgt tgagtttatc tctgataact tcatctttat taatggtagt
    10321 aagttaatcg atccaatgtg gcaattttct acacaaatct ctcagacgac gggaatcggc
    10381 gatgaagaat atggattcaa aatcaaccta gtgatgcaca cagcaggaat gatcgaacgt
    10441 attattcgga atgaaccatt gactgtggaa gaaaacgaat tgacaaatac aacgaatgat
    10501 cctctttata gccaattggc agcctcagtt gttctgttgg aagatcagat aaaagtaaaa
    10561 gtgccgatag aagaaatgta ttatctgcta cgtctagtcc ataatcagtt agataaaaaa
    10621 gaatacactg tgccatgaat acacaaaaac gatacacttt tatagtgtat cgtttttgtg
    10681 tattggttaa gaatccttta aattcaatta gtgtatcgta aaaaagttgg cacgcttttt
    10741 gcttatagta gtgtgtgcta ggaggtggtt gttcacacag aaatgatagg ggtttctgtg
    10801 aagtagtgag gaagaaggag ttttgcagat ggatattcga ttggcacgta tagatgatcg
    10861 tttaatccac gggcaagtcg caacagtttg gtcgaagcaa acaggtgttg aacgtatcat
    10921 tgtgatcagc gatgtagtcg ctcaagataa attgagaaag tttctactaa aagaagctgc
    10981 accgccgggg atcacagcaa atgtaataac cgtgcagaaa atgattcaaa tctatcatag
    11041 tggattatta tcacgagcaa aagtgatgct tttattcacc aatccccaag atgttgaagc
    11101 ggttgtccgc tgcggtgtac agttgaagtc gttgaatatc ggcggtatga gttttactga
    11161 aggcaaatca atgattacca attttgtttc agtaaatcaa caagatattg attcctttcg
    11221 ttacttgaac agtcaagaag tggagttaga gattcgaaaa gtacctgctg acaggaaaat
    11281 ccttttgatg gaactactag cgaaaatcaa aaaggtgtag caagaatggt tgttggaaaa
    11341 ggtcaaataa ttctgttgta atggttagca attattaata tttttgaatt tgttacaaca
    11401 aaagtaaata ggaggtataa atatggtagg gattatccta gcaagtcatg gacaatttgc
    11461 tgaaggaatc ttacaatccg gttcaatgat ctttggcgaa caagaaaacg tcaaagccgt
    11521 gattttgaaa ccaagtgaag gtccagatga cttgagagcc aaattggaag aagcggttgc
    11581 ttcttttgat aatcaagacg aagtgttatt cttagttgat ttatggggcg gaacaccgtt
    11641 caatcaatca aacacattat tcgaagagca caaagataaa tgggcaatcg tcagcggttt
    11701 gaatttacca atgttgattg aagcctacgc atcacgcttt tcaatggaat ctgctcatga
    11761 aattgcagca cacatcattg aaacagcaaa agacggtgta aaagtaaaac cggaagaatt
    11821 agaaccggca gaagcaccga aagcagcagt tgaagacgca caacctaaag gagcacttcc
    11881 agaaggaact gtggtcggag acggaaaaat caaatatgta ttagcacgtg ttgactcacg
    11941 tctgttacat ggacaagttg cgacagcttg gacaaaagca gtgcaaccaa accgtattat
    12001 cgttgtttca gatgcagtat caaaggatga tctgcgtaag agactaatcg aacaagctgc
    12061 tcctccaggt gtaaaagcaa atgttattcc tattagcaaa atgatcgaag tagcaaaaga
    12121 ccctcgtttt ggaaatacca aagcattgct tttatttgaa aatcctgaag acgtattgac
    12181 agctgttgaa ggtggcgtag acatcaaaga attaaatgtt gggtcaatgg cgcattcggt
    12241 cggtaaagtc gttgtaagta aagtattgtc tatgggacaa gaagacgttg aagcgtttga
    12301 gaagcttgaa caaaaaggtg tgaaattcga tgtacgtaaa gtacctaatg attcacgcga
    12361 taacatggat gatattttga aaaaagcaaa agctgaatta gcaaaagcgt aataaataat
    12421 attaaaattt ataggaggct tatcatgtct attatttcaa taattttagt cgtattagtt
    12481 gcctttctag ctggtatgga aggtattctt gatgaatttc aatttcatca accactagtg
    12541 gcatgtacat tgatcggttt agttaccggt aacctcgaag ctggtatcgt acttggcggt
    12601 acgcttcaga tgatcgcttt aggttgggca aatatcggtg ccgctgtagc accagatgct
    12661 gcattagcat cagtagcatc agcaattatt ttagttttag gtggacaagg tgttaaaggt
    12721 gttccttctg cgatcgcgat tgccgttcct ttggctgttg cagggttgtt cttaacaatg
    12781 atcgtacgta caatcgctgt tccaatcgtt catttgatgg atgcggctgc tgaagaagga
    12841 aacatccgta aggttgaaat gtggcacatc attgctgtat gtttacaagg ggttcgtatc
    12901 gcggtcccag cagcagcgtt gctatttatc ccagcacaaa ctgtgcaatc attccttgaa
    12961 tcaatgccag catggttaac agatggtatg gctatcggtg ggggaatggt cgtagctgta
    13021 ggttacgctt tggtaatcaa catgatggct acaaaagaag tatggccatt cttcgttatt
    13081 ggtttcgttg tagctgcaat ttctcaatta acattgattg ctctaggtgc tttaggtgtt
    13141 gcattagctc ttatttactt gaacctttct aaaatgggtg gttcatcaaa taatggaggc
    13201 ggaagcaact ctggtgaccc tttaggcgac attttgaatg actattaatc ttgaaggagg
    13261 aaaataaaat ggcagaagaa aaaatcaaat tatcaaaaaa agatcgttta gctgttgcgt
    13321 ggcgctctac cttcattcaa ggttcatgga actacgaacg tatgcaaaac ggtggctggg
    13381 cattttctat gattccagca atcaaaaaat tatacaaaac aaaagaagaa cgttctgcag
    13441 cattaaaacg tcacttggaa ttctttaata cacacccata cattgcttct ccaattttag
    13501 gggttacatt ggctctggaa gaagaacgtg cgaatggcgc gcctgttgat gatgtagcga
    13561 ttcaaggggt taaagtaggt atgatgggtc ctttagctgg tgttggtgac ccagtgttct
    13621 ggtttacagt acgtccaatg ctaggtgcac taggtgcttc attagctatg ggaggaaata
    13681 tccttggacc aatcatcttc ttcttaggtt ggaacattat tcgttgggca ttcatgtggt
    13741 atacacaaga atttggctac aaagctggtt ctaaaattac tgatgacctt tctggcggat
    13801 tgttacaaga cgtaacaaaa ggtgcttcaa ttttagggat gttcgtatta gctgcccttg
    13861 tacaaagatg ggtatcaatc aaattcttac cagttgtatc aactgtaaaa ttagataaag
    13921 gtgcttacat cgaatgggat aaattgcctt caggtggtga aggaatcaag agtgcatttg
    13981 aacaagttaa taatggctta gccctttctc ctgaaaaggt aacaacatta caagacaact
    14041 tagaccaatt gattcctggt ttagctgcat taggattaac attgttatgt atgtggttgc
    14101 ttaagaaaaa ggtttctcca atcgttatca tccttggttt attcgtagtc ggtgttgttg
    14161 gacacgtcat cggactttta taatccataa aaatatagct cgggcgttgt cccgagcttt
    14221 ttttatatag tatactgttc ataaacaaat atctggaggg agaaaatggt tcaatcaatc
    14281 aatacaaaag tagacttggt aattgatgct acttcgttta cgggtctgac agattacggg
    14341 caaatcatga taggtgataa tggttttgag ttttaccatg cgcgagatag acgcaaatat
    14401 atccaaattc catgggaaga agtggattat gtactagcat cagtcatgct aaagggaaaa
    14461 tggattcctc gatatgccat tcaaacgaaa aaaaatggta catacacatt ttcttccaaa
    14521 gaacctaaaa aagtgcttcg tgcgatcaga gaatatgtag atgaagatag aatggtacaa
    14581 tcattgagtt tctttgatgt agtgaaacgt ggtattcgta gtatgtttaa acgcaagtaa
    14641 aaaataaaga aaacttctaa gaggaggttt tctttatttt tttactttgc agaagaaaat
    14701 atgaaatagc aaaaccaatt atttgtcccc tttaaatccc atatgctaaa attgattagc
    14761 aaagaaggag gttatattat gagttggtta tggacattga ttgttggagc cgtgatcgga
    14821 gcaattgcag gtgcgatcac cagtaaagga aattcgatgg gctgtgtaac gaatatcatt
    14881 gctggactag tcggttcaag tattggacaa gcgattttcg gcgaatgggg accaaaagct
    14941 gcaggtatgg cacttattcc atctatttta ggcgcagtga ttttggtagc agttgtctca
    15001 tttttctttg gaaaacgatc ataagttttt acaaaatagt aaactaaaag gagttgcagc
    15061 gagactgtga ctcctttttg gctttattta tttaaaataa aaagaagttt tccggaaatt
    15121 tttatttaac tgtcgtgtac gaagaaaagt ttgctataat ggaaaaaagc gtgcaaagaa
    15181 agagggagtt tcattatgat gctacgtgta gaaaaattgc gcaaaaaaat gcaagaagaa
    15241 aatttggatt catttttggt taccagtccg tacaatcttc gttacttaac gaactttaca
    15301 ggtacgacag gattagctgt tattactttg gaaaaagcat tttttattac tgattttcgt
    15361 tataccgagc aggcggcagc gcaggctcaa gggtttgaaa ttattaaaaa tgtcggacct
    15421 atttttgatg aggtagcaaa cttagtacgc aaagaagaac tgcactcatt agcttttgag
    15481 gaaacaacag tctcattttt agaatattcg gttttagaag aaatcatcga tgcggaatta
    15541 gtacctgttt cacagatgat cgaagaactc agagaagtga aagatgaaga agaaatcgct
    15601 attatcgaaa aagcatgtca tattgcggat atggcatatg atcacatcct gaaaatgatc
    15661 caaccgggca tgactgagat tgaagtcgca aatcaactag acttttatat gcgttctctt
    15721 ggcgcttcag gtgtttcttt tgatacgatc gttgctagcg gattgcgttc ggctatgccg
    15781 catggtgtag caagcaaaaa aatcatcgaa caaggtgact tgatcacatt ggactttggt
    15841 tgctattacg aagggtacgt ttccgatatg acacgtacat tcgctattgg tgatcctggg
    15901 gaaaaattaa aagaaattta tcaaatagtc ttagatgctc aattagcagt tattgatgct
    15961 gcaaaaccag gaatgactgg tgtccaatta gatgcagttg ctagagatta tatttcaaaa
    16021 catggttacg gggaagcatt tggacattct acaggacatg ggatcggttt agagatccac
    16081 gaaggaccaa acgtttcttc tcgtgcagaa aagcaatttg taccaggaaa tgtgatcact
    16141 gatgaaccag ggatctactt accaggaatc ggtggagtac gtattgagga tgacttgttg
    16201 atcacagaag atggaaatcg tgtgttgact cgttcaccaa aagaattgat catcttatag
    16261 tttcatcaat agaagaagta agcgtaaaaa tcagtgttca cagactttgt tatagaaaat
    16321 cactcgattt aatgatacac tatagaagaa cttttaagct gtaagagata ataaagttgg
    16381 gtgttttctc caattcatag cagtttgcag ctgatcggcg gcagttttct taccacgtga
    16441 tcactatgcc gcatcataga gaattcggat gtatctcgtg cttaggcaaa gggaggaata
    16501 aataaatggc agatgaaaaa aatttgattt taaacgcaaa ccaagattta ggagagatcg
    16561 tgatcgctcc tgaagtcatc gaagtaatta tcgggattgc agcttctaaa gtagaaggcg
    16621 tatacggaat gcgcggaact tttgccagca atgtcactga attattagga cgtgcagctc
    16681 acggaaaagg tgtttactta gcaaacgagg aagatggctt gaaagtagat ctttactgtt
    16741 atctagaata tggtgtttca gttcctaaag tagcaatgga aatgcaggaa cgggtcagcc
    16801 agcaagtcct tttcatgacg gatatcgatt tatctgaagt caatatccat gtagtagctg
    16861 ttgttcctga aaaattagaa caaccaacaa tcgaagaact ttttgaagac gaagaggaag
    16921 agaatgaata aagaattatc aagacacgag attcgggaaa tggcattgca ggctttattt
    16981 ccattagact tcaatgcaga cctaacaaaa gaagatgcga ttttcaacgc aatcgaatta
    17041 gaccaccgcg acatgatcaa cgaagatgaa tcagagtttg ttcctgttta tcttgatacg
    17101 ctagttggcg gtgtctgtgc aaaaaaagat gaattggata aagtgattga aaaacattta
    17161 aaaaacaatt ggcacatcaa tcggatctca aaaatggatt tggtgatctt gcgtatcgcg
    17221 atttttgaaa tgacttatgt gacagatgta ccagcggctg ttgctttaaa tgaagcaatc
    17281 gagttggcaa aaacatttag tgatgaccgt tcacgtaaat tcgtcaacgg tgttttatcc
    17341 aatgtattga aagaattaga agctggggcg taagtcctag cttttttcta tgaatcatca
    17401 taaacctgtt ataaatcggg aaaattctat ggtagaatga aaacacaaaa aatatctggg
    17461 agtggttaga tggcagaatt gattaacgga aaagaattag ctgaaaaaat gcaggcagag
    17521 attgctgaaa aaatccaaaa attgaaagat aatggtatcc atcccggatt agtcgtttta
    17581 ttggtagggg aaaatccagc cagccaaatc tatgtaagaa ataaagaacg tgtagcaaaa
    17641 gaaatcggaa tccattcatt ggtagagcgt tatcctgaga cgatttctga agacgaacta
    17701 cttgcagaaa ttgaaaaata caatcaagat cctagttttc atggtatatt ggtccaatta
    17761 ccattgccaa agcatataga tgaagaaaag atcctgttag ctatccgccc tgaaaaagac
    17821 gtagacggtt ttcatccgat gaatttaggc agactccttg cgggtaaacc ggacaagatt
    17881 ccttgtactc catacggcat catgaaaatg tttgaagctt attctatcga tccggcgggt
    17941 aaacgagcag tagttatcgg acgaagcaat atcgtcggta aaccgatggc tcagctactc
    18001 ttgatggcag atgcaactgt tactgttgct cattctaaaa ccgaaaatct ggctgaactg
    18061 gcacaagaag cagatatttt agtcgtagca ataggacgtg gacattttgt cacaaaagaa
    18121 ttcgtaaaac caggtgctgt agtgatagat gtagggatga atcgtgatgc taacggtaag
    18181 ctgatcggtg atgtgaaatt tgatgaagtg gagcctattg caggctggat cactcctgtg
    18241 ccaaagggtg tagggccaat gactatcacg atgctgatgt atcaaaccgt aaaatctgcc
    18301 gaagcttttg gagcaggtga atagatggca caagaatatt taactgtcac tacattaacg
    18361 aagtatttga aaagaaaatt tgatgccgat ccttatttag aacgcgtata tttaacaggc
    18421 gaaatatcga atttccgttt acgtgccaat gcccatcaat atttcagctt aaaagatgac
    18481 catgcaaaaa tatctgctat tatgttcaaa ggagcattcc aaaaattacg attccagcca
    18541 aaagaaggaa tgaaagtcct tgtcgttggg cgtatttcat tatatgaagc gggaggttct
    18601 tatcaaatct atgtagagca catggagcct gatggaatag gtgctttata tcaagaattg
    18661 gaagaacgaa aagaaaagct gatgaaggaa ggcctttttc aagggcctaa aaaaatgctg
    18721 ccacgtttcc caaagcgaat cgcggtcctt accagtccaa gcggagcggt tatccgcgat
    18781 atcatcacga cggtgaaaag aagatatcca attgtccaat tggttttgtt cccaacagtt
    18841 gtacaaggaa accaagcagc ggacaatgtt gtcaggaata tccagagagt agaagagatg
    18901 ggcaatttcg atacaatgat catcggtcgt gggggtggtt cgattgagga tttatggccg
    18961 tttaatgaag agcgggttgc tcgggcgatt tatcaagcta atacgccggt gatttcatct
    19021 gtaggacatg aaacagatac cacgatcgct gatttagtag cagatgttcg ggctgccact
    19081 ccaacagcag ctgcagaatt agctgttcct gttttgtcag aagagttgat gcgcattcaa
    19141 gaacggcaag ctagattgca gcaggcgttt gtacgccaga tccagcgcaa acaagaacgg
    19201 ttcgagcgtg ctcgacgttc ctatgtattt cggcagcctg aaagattgta tgaaggtcag
    19261 accatcaaat tagaccagtt gaaacagcga ttatatcaag ctacacagca aatttatcat
    19321 gcgaaagaaa aacaaacgat cgcattaagc caccaattgg aacaagtggc tccgctttat
    19381 cgagttaaag cagctcgcca agaaactgct tatctagaaa aacgcttaga agagaaaatc
    19441 caacaatata tgaaacaaca gcagcagcgt tttgctcaag ctgttcaatc tcttgatttg
    19501 ttgagtccat tgaaaatcat gggaagaggc tacagctata cgacgttaaa tgatcaagta
    19561 gtaaaaagtg tcaccgagat tcatcaaggg gatttgctga cgatccatta tcaagatgga
    19621 atcgttaaag gacaagtaca agagatagac aaaggagaat aaaaatatgg aaaatccaac
    19681 ttttgaagag tcactgcaag aattagaaaa aattgtcatg cagttagaac aaggagacgt
    19741 tccattagaa tcagctctag attcgtttaa acgaggaatg gaattaagca aacattgcca
    19801 agatacatta gctaaagcag aagagacctt gacgaaaatg atgacagaat caaatgaaga
    19861 agttgatttt gacggaaacg aggaaagctg agatgagatt aactgacttt tcagctattc
    19921 atctacctgc tgtagaaaaa gaaatcttgt cttttttaga tgagcataca acggatagag
    19981 gactctatga tgcgatgagt tattcagtga aagcaggcgg gaaaagaata cgtcccttat
    20041 tacttttaac ggccgttgct tcctttgatg agcctattga tgttcccgtc tatcaagtag
    20101 cagctgcttt agaaatggtg catacgtatt ccttgatcca cgatgatttg cctgcgatgg
    20161 acaatgatga tcttcgtaga ggaaaaccta ccaatcataa agtattcggc gaagcattag
    20221 ctatattggc cggagatggt ttgcttacag gagcattcca gctaatcagt atggcacact
    20281 taggtaattc tcctaagctt ctgctgcttc agcaattagc tgtttgtgcg ggatcacaag
    20341 gtatggttgc tgggcaagct gcagatatcg agggcgaatc gaaaaagctt tctttagaag
    20401 aattagcatt tattcatgaa agaaagacag ggcatttgat tcgttatgcc ttgctggctg
    20461 gtggcatatt agcgaagcaa cctgaagaaa tcttattgct acttcaacga ttagccgaac
    20521 atttaggttt agcttttcag ataagagatg atctactcga tgtgattggt acaacgaaaa
    20581 cattagggaa aacagctggt aaagatgaac gtatggaaaa aaatacttac ccgcgtttat
    20641 taggcctaga aaagacacgt gaggcgttgg agattgagct gttatccgcc aacaagatca
    20701 tagacaaatt agaagaggac gttttttctt ttgatggcgg gctgatgcgc cagatgatca
    20761 agcaatttga tgtggagaaa tgagggaaag aatggaaaaa gaacgcgtag atgtattagc
    20821 ggtcaaacaa ggattatttg aaacaagaga acaagccaaa cgttcagtga tggctggttt
    20881 gatttacaac gaaaaaaacg agcggtttga taaaccggga gaaaaaattc cagtgagtag
    20941 tgaattaaga gtcaaaggta aaaaacttcc ttatgtttct cgagggggat taaaattaga
    21001 aaaagcactc aaagcatttg atctatctgt tgaaggaaaa acattgttag atattggtgc
    21061 atccactggc ggatttactg atgcagcttt gcaaaatggc gcaaaaatga gttatgcttt
    21121 agacgttggc tacaatcagc tagcttggaa aataagacaa gatcctagag tcgtcgtaat
    21181 ggaaagagtg aattttcgtt atgcaaaacc cgaagacttt accttaggaa ttccagaagt
    21241 tgctgttatc gatgtttctt ttatttcact aaaactgatg ctgcctccat tacatgctat
    21301 tttaaaagaa gagggagaag tgattgcttt aatcaaaccc caattcgaag caggcagaga
    21361 agctgtaggg aaaaacggaa ttgttcgtga ccctcagacc catcaaaaag ttttagaaga
    21421 cattttgtca tttgcggcac aaggtggata tgatgtgctg gaattaagtt attctccgat
    21481 cacgggggga gaaggaaata tcgagttctt ggctcacttg aggaaagtac ccgaaagcgg
    21541 aacaatcaat tcagctatta atatggcaga agtggtttca aatgcacacg aacagtttga
    21601 ccataaataa attgtaactg tgaaaatcaa gtgagaagta tgcggattct tctttattat
    21661 accaaggaaa taagctatca ttgattaaga agaatatgct aaggagcgaa gacgtaatga
    21721 gaaaaaaaga tcggcatcgt ttgattactc gattgttgag tgagcaagat atccgaaagc
    21781 aggaagaatt tgttgaaatc ctgaaaagca aaggaatatc tgttactcaa gctacgattt
    21841 ccagagatat aaaggaactg aaactgatca aggtaccggc ttcagatggc ggctaccgtt
    21901 atagcatacc agctgaaaca aacgaagatg taaatactaa attggaaaaa ctattaaaag
    21961 acgctttcgt ggcagttgac caaatggaaa aatttgttat tttgaaaaca ttgccgggaa
    22021 atgcttcggc agcagcaaat ttgattgata aacgttacaa gaaagaatta ttttcgatta
    22081 tcaatgatga cgataatgtt ttgatgatta cacgaactga agaccaggca gttttattga
    22141 agaaagactt cctgcattac ctttaagttg aggagcgaat gtgatgctac aagaaatcag
    22201 tattactaac tttgcgatta ttccggaatt gcggctttct tttcacgaag gaatgaccgc
    22261 gctgaccgga gagactggtg cgggtaaatc cattattatc gatgctttag gtctgttagc
    22321 aggaggaaga ggttcaagcg attatatacg tcaaggagca gaaaaatgtg tgcttgaagg
    22381 gttgtttgaa ctaccaaaac aagaaggatt ctctgaactg atggttgaat tagggatcga
    22441 aacagatgag gacaatctga ttgttcgacg ggatatgtcc ttaactggaa aaaatgtttg
    22501 tcgagtgaat ggtcatatta tcacattagc taatctgaga agaatcggca gttatttagt
    22561 agatatccaa ggacaaaacg aacatcagga actgctccag cctgaatcac atcttgcttt
    22621 attggatcgt tttggagatg tagctttcca acagaagaaa aagagttatc agcaagaata
    22681 tatctcttat cgtgagctgg aaaaaagggt acgaaaagtg cagcaaaatg aaaaatccta
    22741 tgttcagaga atagatatgc tccatttcca gcaagaagaa atagctgctg ctgatttgca
    22801 agtcggagaa gaagagaaat taaaagaaga aagagaaaaa cttagcaatt atcaaaaaat
    22861 tgctgatgga ttagctgcag gttacggagc actgacgaat aatgaacaaa acagtgtaga
    22921 tggtgttggc ttggccgttt cagaactgca agggatcgcc catctagatg tggagtatga
    22981 agcaatttat gaaaatatcc aaagtgctta ctatttgctg caagatgcaa tcggcgatat
    23041 gagtcgtcag attgatttat tggagttaga tgagagtcgt ttagaagaag ttacgcaacg
    23101 tttagaatta atccgacagt tgaaacgtaa atacggcgaa tccattgagt caatattggc
    23161 ttactatgac gaaatcaccg aagagctagc ttcttctgat ttttcagaag gtcaattgga
    23221 caaaatgaaa gaggaactag agcaaaaaga gcttctactt cagcaacaag ccgcagatct
    23281 tcatgaagcg cgtaaaaaaa tcgcaaaaga attggaaaaa tccattctgc atgaattgaa
    23341 aagtctgtat atggaaaaca ctgagtttga ggtacgtttc ttaaaagaag aaaatagaca
    23401 attgaatcat gatggatttg accaaatcga attttacatt acgaccaatc caggagagcc
    23461 actgaagcct ttggtgaaag tcgcttctgg tggcgaactg tctcggatgt tgcttgcttt
    23521 gaaaactatt ttttcttctg agcaaggtgt gacaagtatt atttttgatg aagtggatac
    23581 aggtgtgagc ggaagagttg cacaggccat cgcagataaa atttctcaaa tatcgaaata
    23641 ctctcaagtt ttgtgcatca ctcatctgcc tcaagttgct gcagtcgccg actatcaata
    23701 ttatattgtg aaagcagtga ttgatggacg tacgcaaact tctgtttcag agttgaaaac
    23761 aaaagaaaga gaagaagaaa ttgcacgtat gctggctgga agtgagatta caaaactcac
    23821 actcgaacat gcaaaagaac tattgaaact cgcaaaaagg acctgagcca cctcgcagtg
    23881 gttcaggtcc ttttcagcag ctgatagcta tattttattt ttacatgaca ctcattacag
    23941 cagtcaatac aacagatcca atcgttaaaa tcagcattgt ccaaatgaca aattttgtta
    24001 ctttagcaaa agtacttgtt tgtttttttt cacgcattgt tttcaacctc gtcattaatt
    24061 agttgattca agtgtacaat cttttttcca tttccacaag agggcttttt tataaaaact
    24121 tccctttttt taaataatag gtagttacga tacaaataaa taatgttaat agaactagcc
    24181 atataaaggc atcatccctt tcagcaatag gtaacttaat gttcattcca tagatactgc
    24241 cgacgattgt cggtatagtt aaaataatcg ttaaagaagt taaaattttc ataatattat
    24301 tcagattatt cgatacgatt gctgaaaaag tctgattcat ttgttctact agttttaatt
    24361 gaatgcgaga agtagtcatt gcctgctctg tttccaccaa tacatcacgt aaatgatttt
    24421 tcaaagcatg attttcttgg aaaagtttat tttgcgctag ttctttcaat gcatctaaat
    24481 tagcagaagt cgcttctctg aaataaacga ggcttttttg gatatccatg atttgataaa
    24541 gttgtttgtt ttcagtggat acttgaagtt tggcttccaa agtatcgcat tgagattgaa
    24601 gcgtagctaa ataactgttg taagagagtg tgacctgcca aatgaattga aagaagatat
    24661 tgagattagg cgaaatatca ctttttggaa aggtctggct aaagactttc tttaaaaaca
    24721 gtggttcatg attcgtaatt gtgataagct tctttttggg cgtcaaaata atagagagcg
    24781 ggtatgtatc cacttgaaaa taaccgctag gacttgtttt agcaaaagga tattgtaaca
    24841 ataataacgc tggctttgtc aactttgatt gattgaattc ttctgtacgg gaattttctg
    24901 catcgtccaa tatggaagtg atgtaatctg acggaatttc aaattgatgt gtcagctgat
    24961 gaatctcttc ttgattagga cgttctacac aataccagat cgtttggtca gtttcctttc
    25021 cggtattacg cagtttctca ttatctaatg taaaatattt gatcatgatt ttcctcctgt
    25081 tgcctgtttc ttttattata acggataggg aattggaata catggaatgt gtttgtaaga
    25141 tatagaatat aactgcataa atttaaataa aatgaaacag ttgtgtaaag tatatacagt
    25201 atacttgaag caacgataaa aaaggagttt acgttcatgg gcacattcac accacttatc
    25261 ttgttttggt atatttaccc ggtaatcgtt ttgtttggtt gccaatttct tgtaaaatta
    25321 ttttcgttaa atcgtcgttt taaaataaaa gcccctgatt tggcgattcc ttttttgtgg
    25381 ggcggtatgc atgcattgtc tagaaataca gggacaattt ctatcttacc gtttttcttt
    25441 atttctgttt tgctaatggg aatttgtatt gctgttttcc aagcatatta ttatgaagaa
    25501 attatttatc cgcgttattt caagatgact tggcgtttgg tttttctgtt gacaatcgtc
    25561 ttgtacgctg tcttgatcat tgtgaacata ctttcttacc tatgaaaaaa atagaaaagc
    25621 tgtaaaaagt cttttaaaag gctttttgca gcttgatttt taaaaaaatg tttatgaata
    25681 aaaaaagttg ttcccggatt tcccagaaaa ggtggtagaa agtgggggat tgtggtagac
    25741 taattttagt tagtggagga atggggggct ttcaatgttg atgggtgaat tccaacataa
    25801 tattgatgca aaaggacgtt taatcgtgcc ctcaaaactt cgtgaagaac ttggcgaaaa
    25861 gtttgttctg acacgcggat tggatggctg tcttttcggc tatccgatga gcgaatggga
    25921 aaatctggaa gctaaactga acgaaatgcc gttggctaaa aaagacgcac gtacttttgt
    25981 gcgattcttc tattcggctg ccactgaatg cgaacttgat aaacaaggac gaatcaatat
    26041 acccagcacg ttaagaaatt atgcagcttt gacaaaaggt tgcgtagtca tcggtgtatc
    26101 taaccgaatt gaaatttggg atgagacacg atggcaagat ttttctgcag cagcagaaga
    26161 gaactttgac gaaattgctg aaagtatgat tgatttcgga ctataagaat aggaagaggc
    26221 tgactaatga ctgaaacatt tcaacactat acagtcatgt tgaaagaaac ggtagatgga
    26281 ttgaacatca aaccggatgg cgtttatgta gactgtacac tcggcggtgc cggacatagt
    26341 gaatatttat taagccaact aagttctaaa gggcatctgt atgcgtttga ccaagatcaa
    26401 aaagcaatcg atcacgccaa gctgcgttta gcttcttatg tcgaacaagg tatggtcaca
    26461 tttatcaaag cgaacttcag agaattagaa gaacgcttga gtgagcatgt agataaggta
    26521 gacggcattt tgtatgatct aggggtttcc tctcctcagc tagatgaagc agaacgtgga
    26581 tttagttatc atcaagatgc accgttggat atgcgcatgg atcagtcagc tcctttatcc
    26641 gcttatcacg taattaatga atattcctac catgaattag taaagatttt cttccgctat
    26701 ggcgaggaga aattttccaa acaaattgct cgtgaaatcg aacgagtacg aaaaacaaaa
    26761 ccaatccaaa caactggtga gcttgtcgaa atcattaaac aagtcatccc agctcccgca
    26821 agaagaaaag gcgggcatcc tgcaaaaaga atttttcaag ctgtgcggat tgcagtaaat
    26881 gacgaactcg gtgccgaaga ggcctcttta gaacaggcaa tccggttatt gaaagtcaat
    26941 ggaagaatca gcgtaatcac attccattca ttggaagaca gaattgtcaa aagtatgttt
    27001 aaagaatata gtacggtaca agatctacca ccgggacttc ctgtcatacc tgacgaattc
    27061 caaccagaac tgaaagtcat tactcgtaaa ccaatccttc cttcagagga agagctaaca
    27121 gaaaacaatc gatcacgcag tgcaaagtta cgtattgcag aaaaaataag aatgaaagag
    27181 gagtagaatg atggcagaat taaagaaaat gcaagattac gaatatgatt tacctgaatt
    27241 ggtggatcag ccggaacaac aaatgcctga ttcttcttcg aatcaagaaa tcattcttcc
    27301 acaatcaccg gcaaaacgat taaagcagat ttctgctttg gaaaaaattg ctgtggcaag
    27361 cgtggcgatt gctattgttg ctttatgtgt tttaacagtt atgctgcgca caaatatcag
    27421 cggagctgaa aaagaaatca cgtcaataca aacagaaatc aatgataaaa agcaagaaaa
    27481 aacgagttta gaacaagaaa aaaacgaact ttctcgtact gaacggatta aacaaatcgc
    27541 agaagaaaaa ggattgtcta ttaatgacga caatttaagg aaagtgaagt aaatgagtct
    27601 taaaaataaa attcggcggt ttatggaaaa gaaaaatcta aatccgatga acaatcgcaa
    27661 aaaagtaggt atcattttat ttgctacgag tattggattg ttctttttat ttgccgtccg
    27721 tttctcgtat atcgttattg gaggtcatgt tgctggtacc tcattagctg aaaaaacaaa
    27781 acagttgtac caaggaagtg aagtagtaaa agcaaaaaga ggaacgattt atgatcgtaa
    27841 cggtgtggcg ctcgcagaag atgcctcctc ttattcaatt aaagcgatac tctcaaaaac
    27901 atatacctct ggggataaaa aactgtatgt tgaagaaaag aattttgata aaatagcaga
    27961 aatcctgcat aagaatcttt ctatagacaa aaaagatgca ctgaatattt tagaggacgg
    28021 cgcaaaaaaa gagctgtatc aagttgaatt tggtagctat gggaaaaata tctcccaaga
    28081 aaccaagcaa aacatcgaag cggacatgaa aaaagaaggt gtcgctggtc tttattttgt
    28141 cgaccatcag gctcggatgt atccaaatgg cgtcttttct tctcatttta ttggatacgc
    28201 agttccagac aaagatgaga atggcttagt tggtaaacta ggtttggagt ctgcttacaa
    28261 tgatatcttg agcggtaaag atggtaagat tatttatcaa aaagataatt ttcaaaatcc
    28321 tctgccaggt acagtggcag aagaagaaaa agctgtcgac ggacaagata tctacacgac
    28381 tttagacagc agactacaaa gttatttaga aacactgatg gatcaagtaa atgaagaata
    28441 tcagccagaa gaattgacag cagtcctgat gaaagcaaaa accggagaaa tcttggcaat
    28501 gggacaacga cctactttca atcctgaaac aatggaagga ttgactggaa aagatgctat
    28561 ctggcgtaat ttcttggtac aagatagtta tgaaccagga tcaaccatga aagttttcac
    28621 tactgcagct gctatcgaag aaggagaatt caacgagaat gaaaccttcc aatctggaaa
    28681 aatccaagta gaagatgcta cgatcaatga ccatgacttt ggtgaaaaag gtgtgctgac
    28741 catgcgtcaa gctctttcat ggtcaagtaa cgtcgggatg gtcatactgg aacagcgatt
    28801 aggcggacga tggtataact atttacaaaa actgggattt ggacaaagta ctcattctgg
    28861 attagatgat gaagtaaacg gtgctttacc tacattaaat attgtcgacc gagcaatgag
    28921 tgcttatgga caagcggtag gtgtgacgaa tttccaaatg atgaaagctt tcacttctat
    28981 tgcgaataac ggtacaatga ttcaacctcg atatatcagc aaagtggtgg atccccaaac
    29041 tggagaagag cgaacaactc aaacagaagt tctagggcaa cctttttcaa aagaaacaac
    29101 agaaaaagtc cgtgaatata tgagagatgt tgtcgaaagc gaaaactacg gtagtgttta
    29161 tggtgtatat agtgtacctg gatacaatgt atcggcaaaa accggaacag cccaaatcgc
    29221 ttctgataca gggggatatc aaacaggaga tacagcctac ctgtattcga ttgtggagat
    29281 ggttccttct gaagatccag actatgtctt gtatctaaca atgaaacacc ctaagacata
    29341 tgatcgaatg gcattggcta aaatcgcaaa tccattgatg aaacgggcaa tggattttaa
    29401 agaaactgaa gaagattcag atacagaaac aaaaacagaa aaagtatccg tagccgatta
    29461 tcgtaatctt gaagcggacg ttgcagcagc tgatgctcaa aaaagtggac tgcaacctgt
    29521 agtgatcggt aatggtaaaa aagtacaaaa acaatctact gccaacggtg atcagttgat
    29581 ctctggagaa aaactgatcc tatatactgg tggtgataag ctgatgcctg acgtaacagg
    29641 atggtcaaaa gcagatatca tgaagctggg gaaaatcctc ggtattgaag ttagttttga
    29701 tggagatggt tattgtgtca aacaagaact tgctccatat gaaaaaatca ctgaagacaa
    29761 acttagtttt actttagaag aataagaaga gaaataggag agaaaagagc atggaatgga
    29821 cacaagcatt gattcccatt gtcagcagtt gtgcaatgac aattgccgca atgccgctat
    29881 ttattggtta ttttcaaatg aaaaagcaag gtcaggcgat tcgtgaggag ggaccaaagt
    29941 ggcacaatag caaagccgga actcctacaa tgggcggctt agtgtttctg atcggctcta
    30001 ttttgacagg aatttgggtt ggagcttggc aaaaacagct aactcctact cttttcatct
    30061 tgctttttgt attagcgtta tacggagtaa tcggctttct tgatgatttt ataaaaatat
    30121 tcaagaaaag aaacatggga ctgaactcga agcagaaatt gattggacag attctagggg
    30181 gaatcatctt ttatcttgtt taccgttctg aaggttatcc aggtgtattg aatttcttcg
    30241 gactagatct acctttagga atcatttatg gtattttcgc tctcttctgg cttgttggat
    30301 tttcaaatgc agtaaatctg acagatggca tcgatggatt agttgctggc ttaggaagta
    30361 tctcttttgc agcttacggt ctaattgctt ggcatcagca tcagtatgat gtattaatca
    30421 tttgcttaag tgttttagga ggtttactgg gtttcttcgt ctacaacaga aaacctgcca
    30481 agatttttat gggagatgtc ggttcattag cgttgggcgg acttttagcg gcaatttcta
    30541 taatgctaaa ccaagaatgg acattgcttt tgattggttt gatctatgtt atggaaacag
    30601 caagtgtgat gcttcaagtt acttccttta agcttacagg aaaacgaatt tttaaaatgt
    30661 ctccaattca tcatcacttt gaaatgtgcg gatggtcgga gtggaagatt gatacagttt
    30721 tttggttgat cagcattgtg acttcgttga tcacattatg gatcgtttgg taggagatga
    30781 cgggaaatga aaaaaatatc aacatatgaa aataagaaag tgcttgtctt aggactcgca
    30841 aaaagcggag taagtgcggc caaattatta catgaattgg gcgcacttgt caccgttaat
    30901 gatggaaaac cttttgacga aaatccggaa gcacaagagc tgctttcttt agggataaaa
    30961 gtaataacag gtagtcatcc aatcgaattg ttggatgaag aattttcatt gatggtcaaa
    31021 aatccaggga ttccctacag tcatccttta gtagcaaaag cccaagaaat gggtattccg
    31081 gtaattacgg aagttgagct agcttacgaa gtagcggaat gcccaattat tgggattaca
    31141 ggaacgaacg gaaaaacaac gacaacaacg atgacaggac tactactgaa tgcaggagca
    31201 gatcaaggaa ttgcacgttt agcgggtaat attgggtatc cagcaagtgg tgtagcgcaa
    31261 gaagcaaaat cagaagataa aattgtgatg gaattgtcta gcttccaact catgggaatc
    31321 acagatttcc gccctcatat agcggtgatt accaatatct atgaagcaca tattgattat
    31381 catggtacaa gaaaagaata tgtcaaagct aaatggaacc ttcaaaaaaa catgacagaa
    31441 aaagattatc tgatccttaa ctggaatcag tcagaactac aagaattagc gcaaagaaca
    31501 aaagcacgcg tactgccttt ctcgacaaaa gaagtgttgg aagacggtgt gtatgctgac
    31561 gattactcaa tatactataa aaaagaaaaa ataatggaaa tatctgaact tggcgtacca
    31621 ggtaagcata atgtagaaaa tgcactagct gctatttctg tagcaaaatt gtatggtata
    31681 tcaaatgaag ctatacgaga aacgttgcat tttttccatg gcgtaccaca tcgtacgcaa
    31741 tatgtaggag aaatccaagg cagaaaattt tataatgatt caaaagcaac caatatttta
    31801 gcgactaaaa tggctctaag tggatttgaa acgtctaagg tcgtgttatt ggctggcgga
    31861 ttagacagag gaaatacatt tgatgaatta attccttcat taaaaggaat caaagcaatg
    31921 gtcgtatttg gccaaacaaa agaaaagttg atggatgctg gaaaaaaagc aggcatcgag
    31981 acaatagtaa cagccgattc tgtagaacaa gcagttcctt tagcattaga aaacagtaca
    32041 gatggtgatg tagttttatt atcaccagcg aatgccagct gggatcaata cccaaatttt
    32101 gaaactagag gaaacagatt catggaagct gtgaaccgat taaaatagaa aaagtagaag
    32161 cgagcgataa aaaatgaaaa ttttggttac aggcggagga acagggggac atatttatcc
    32221 agctcttgcc tttgtcaatt atgtaaagac tcaagaaccg aatgctgaat tcatgtatgt
    32281 gggagcaaaa cgaggtctag aaaacaaaat cgtccctgat acaggaatgc ctttccatac
    32341 cttggagatt caaggattta aacgaaaaat ttccatgcat aacatcaaga cggtccgatt
    32401 gtttcttaag agtatccgtc aagcaaaaaa aatcctcagt gattttcaac cagatatcgt
    32461 tatcggtact ggaggatatg tttccggagc ggtagtgtac gctgcagcca aaatgggtat
    32521 tccaacagtt atccatgaac aaaacagtgt accaggtgtt accaataaat ttttaagccg
    32581 atatgtggat cggatcgctc tttcatttaa agatgcggct tccttttttc ctgaaaacaa
    32641 agcagttctt gtaggtaatc ctcgagcaca agaagtagca gatactaaaa aatcagatat
    32701 tttgaaaaca tttgatttgg atccagaaaa gaaaacagtc ttgatttttg gcggtagtca
    32761 aggagcactt aaaatcaatc aagcagtatc agttttttta agtgaatata ttccttcttc
    32821 ttaccaagtt ttgtatgctt caggagatag atattatgaa gagatcctaa ataaaataaa
    32881 tcctgaaata caaaatatta gtgtccagcc ttatatcaaa aatatggcag aggtcatggc
    32941 ttccagtgat ctgctggtgg ggcgtgctgg tgcaacttcg atcgcagaat tcacagctct
    33001 ggggttaccg gcagtgttgg ttcccagccc atatgttacg aatgatcatc agactaaaaa
    33061 tgcaatgagt cttgttcacg caggagcagc aaaaatgatt gcagataatg aactaacagg
    33121 tgaatcacta agtcagacag tcaacgaaat catgggtgat gaagaattgc aaaagcaaat
    33181 gtgccgagct tctaaagaac aaggaatccc tgatgcttca aaacgtcttt atgacttagt
    33241 aaaacaaata atcaaaaaat aaaggcggtg gtcttagtga gtaagaaaaa agaaccaaaa
    33301 aaattgtctc atccgtcttc tgatgaaaac aatttgactc cttggcaaaa ggctaaccga
    33361 aaatatctgg cagaacaaga gatgaaaaac caagagccaa aaaaagaaga cataacatta
    33421 gatacacccc tttcagattc tgaagaagaa aaaacggttc aagaggaaca gcaagaagaa
    33481 agccagtccg agaaaaaaat cgaatttttt gaagagatca agcaaaagaa aaacggtgga
    33541 ccatataacg gttctttttt gaaccggctg cccaatttaa aaagtcaacg gcacaaagta
    33601 ctatttcgtc ggttagcttt gatcatcact gtattgggta taccgctgat ttttttgatc
    33661 tattatgttt ctccttatag caaattacaa gcagttgcag tatcaggcaa taagacagtc
    33721 aacagccaag aaatcatctc tgatacaaaa ttgtcattag gagaaaatgt ttggggacag
    33781 tattttcatc gatctactta tatcgatcgc cttaaaaaag cacagccaag aatcgaaaca
    33841 gcaaatatac atttcaaagg gatgaatgaa ttcgatttgg atgtaacaga atataaagag
    33901 attgctttga tcgcaaaaaa caatcaatac tacccagtga ttgaaaatgg aacagtatta
    33961 gatgaaaaag tagcaaatcc tacaaaaaat ctccctatct tggaagagtt caaagactct
    34021 gcaaaaatca aagaattaac aaagcaatac aatcagttat catcagaatt acaaaaagca
    34081 atttctgaaa tcaagtacac gccaagagca agcaataaaa atctgatcca attgaatatg
    34141 aatgacggca atcaagttat tgtaaatatc aataatcttg cgaaccaaat gaaatattat
    34201 tcgcaagtag ccaaagatat ggatgaaaaa ggtgtgatcg acatggaagt tggtatcttc
    34261 tcgcatcctt acggtagcac tcaagaaacg gaagaatcgg gccaatcgga agaaacaact
    34321 gaaagtgaag aaaatgcgga tcaaacaaac gaatcaagtg aagaggcaga aacgcaaaaa
    34381 gcacaaacac ctggatcgtc tagcacaaac ggatcatctg attcaaatga ttcaagtgaa
    34441 acaagtcaaa tagaagataa tccaagtaca actccgtcaa gtttaaatga aaattgataa
    34501 aacaacttta aaaagtaaga atgtactttc taaaaaccag taatttatgg tagaattgga
    34561 agcagtgaat atatctatta ataagtatat tttaaaacgg acgctagttg ttcgcttgta
    34621 tatggaatag taagtaggag ggaatcccat tcatggcaaa aacaggaatg tatgtaggcc
    34681 ttgatattgg aacgacatct gttaaagttg tcgtggctga atatatcgac agccaaatga
    34741 atattattgg agtaggaaac gcaaaatcag aagggattaa ccgaggcatt atcgttgata
    34801 tcgataaaac ggtccaagcg atacaacgag cagtacgaca agcagaagaa aaagcaggta
    34861 tccagatcaa aggcgtaagt gtcggtttgc cagcaaacat gcttgaagta gaaaattgcc
    34921 agggaatgat tgcagttaac ggagattcaa aggaaatcac cgatgaagat gtcagaaatg
    34981 tggcttcagc tgcccttgtt cgttcaatac ctcctgaacg tcaaatcgtg tcaatattgc
    35041 cacaagattt tactgtcgat gggttcgagg gaatcaaaga tcctcgtgga atgatcggcg
    35101 taagattaga aatgtacggt ctgttgttta caggacctaa aacgatcatc cataacatac
    35161 gtaaatgcgt ggaaaatgcc ggattaatcg tcaacgaaat ggtgattact cctttagcgc
    35221 tgactgaatc aattctttct gacggagaaa aagattttgg tacgatcgtc attgatatgg
    35281 gtggcggcca aacaacaacg gctgttatgc atgataagca attgaagttt accaatcttg
    35341 accaagaagg tggcgaattt gtcactaaag atatctctat tgtattaaat acgtcattta
    35401 acaatgcaga agcgttgaaa ataaactacg gggatgcata cccagaacgg acatcaccag
    35461 atgaagaatt tccagtagat gtgattggtc agtcagaacc agtgaaagtc gatgaacgct
    35521 acttgtcaga aattatttca gctcgaatgg aacagatttt caacaaagca aaagatgcat
    35581 tggaccaaat cgaagcatta gagttgccag gtggcgtcgt attgacaggc ggagctgcta
    35641 gtcttcctgg tgttgtggac ttggcacaag aaatcttcgg cgtaaatata aaattgtatg
    35701 ttccaaatca catggggctg agaaacccgg tatttacaaa tgtaataagt atcgtggatt
    35761 attcagcaaa cttgagtgaa gtgtatcaat tggcaaaaat tgctgttacg ggtgaaacct
    35821 cagcagctcg tcaaatggta gttgaacaag aaacaacaaa cacatatgag agctatgaag
    35881 cacctgaaga aacaatctac gatgagccgg aaccaaaaga gtccggagaa aatgtaaaaa
    35941 ataaaatcaa gggtttcttt acaaatattt ttgattaatt catcctggga ggaataagat
    36001 atggaattct caattgataa taatatcaat gacggcgcag taatcaaagt catcggagtc
    36061 ggtggtggcg gtggaaatgc tgtaaaccga atgattgaag aaaacgttaa aggtgttgaa
    36121 ttcattacag ccaacacaga cgtgcaagca ttaaaaaatt caaaagctga aacagttatc
    36181 caattaggcc ccaaatatac tcgtggttta ggtgctggtt ctcagccaga agttggacaa
    36241 aaagcggcag aagaaagtga acaatcctta cgcgaagctt tagacggtgc agatatgatt
    36301 ttcatcactg caggaatggg tggaggaacc ggtacaggtg ctgcaccaat cgttgcaggt
    36361 atcgcgaaag aattgggcgc attgactgtt ggtgtcgtga cacgtccatt cacttttgaa
    36421 ggaccgaaac gtggccgttt tgctgcagaa ggtatcgctc gtttgaaaga aaatgtggat
    36481 acattattaa ttatctcaaa taaccgttta ttagaagttg tagataaaaa gacaccgatg
    36541 cttgaagctt tccgtgaagc agataatgtt ttacgacaag gtgtacaagg tatctctgat
    36601 ttgatcacag cgccaggtta cgttaacctt gactttgctg acgtgaaaac agtgatggaa
    36661 aaccaaggta cagcattgat ggggatcggt gtggctagtg gagaagatcg cgtagtagaa
    36721 gcaacgaaaa aagcaatctc ctctccattg cttgaaacat ctattgatgg agctgaacaa
    36781 gtacttctta acatcacagg cggtttggat atgacattgt tcgaagcaca agatgcttca
    36841 gatatcgtag caaatgcagc aactggcgat gtaaatatca ttctaggtac atctatcaat
    36901 gaagaaatgg gcgatgaaat ccgtgtgacg gtcatcgcaa caggtatcga tgaatcaaaa
    36961 aaggaacgca agtctagccg accagctaga caagcacaaa tgcaatcacc tgctcaaaag
    37021 actgtattag atatggatca agctaaacca acttcttcag aagaagaaaa cagttttggc
    37081 gattgggata tcagacgtga acaaaatgtt cgtcctagag ttgatgattc caattttgac
    37141 aatatcgaaa aaaaagaatt tgatacattc aaccgcgaag aaacaaaatc tcatggcgat
    37201 gatgaattaa atacgccacc gtttttccgt cgtaaaaaat aaaggagagg aagagcacat
    37261 gattgctgac aacttgcaga aaatcaagca agaaattcag gattcgtgtg ctcttgttca
    37321 tcgtgatccg aaagaagtta cattagtagc tgtaacaaaa tctgttgaat ccgctcaagc
    37381 tgaagaattg gttagagctg gcgttatgaa ttgtgcagaa aaccgagtag ataaattatt
    37441 agaaaagaaa caagatttat ttgattttcc tgaaataaag tggcatttaa ttggtaattt
    37501 acagcgcaga aaggtaaaat tgattgtaaa tgaaattgat tactttcatg cgttggacag
    37561 tttaagatta gcagaagaaa tccagaagcg gttagagaaa gaattatcct gttttattga
    37621 agtaaatgtt tctggtgagg aatcaaaaca tggaatttca ccagatgagc ttttaccgtt
    37681 tgtggaatct ttagctgcct gcacaaaaat caaggttgtt gggttgatga ctatggcgcc
    37741 taaagaagct tctgatgagc aaatccataa agtgtttggt acgctgaaag aattgcgtga
    37801 tcttgttcaa gcaaaaggtt atgcccatgc accatgtagt gaattaagta tggggatgag
    37861 tcaagacttt caaattgcga ttgaagaagg tgcaacattt atccgtgtag gaacagcgct
    37921 attcaaagac gaataaagga ggaacaaaaa tgccgctttt aaacagagga gcaattgcta
    37981 attttttcgg attagctgat gaggaagagt acgaatacca agattatcct gaaacaacaa
    38041 aaaaggctgt acagccaact aaaaaaaata caacacagaa tcaataaact cattctgtat
    38101 ctgctgcgaa tactcatgaa aaacaaaaaa cggcgacttc acgtcctgta acgcatgaaa
    38161 gtattcagcc ggctgaaaag aaaaaagtaa ttgaactaca tcaatcacct acaaaaactg
    38221 tagcgaaaga aagaacagct tcaagaacct ctacacaacc taaagcaaat cttgctggaa
    38281 aaatcactgt aatggaacca agagcttatt ctgaagctat ggcgattgct aaacgaatca
    38341 ttgctggaga agcaacctta gtgaattttc atttagtaga agaaaaacaa gcgagaagaa
    38401 tcgttgattt tctaacagga acagtttatg cactagatgg agatatcaaa cgtgttggag
    38461 atgagatttt tctttgtaca ccgactggga tggagattga cagttcaacc gctcaatctt
    38521 ttgcagatgc gaatttgttt gatctgtaag aggaggaact acgatttata ctattgcact
    38581 attacttcat ttacttcaac gggcagccta tttctatagc attatcttga tggtatatgc
    38641 attgctctct tggtttccag gcgggtatca atcagggcta ggacgattat tagctaagat
    38701 ctgcgaaccg tatatcagtt tgttcgatcg tctgcctttg aggattggac ccttggattt
    38761 cacaatttta ggggcaatcc tgatcttgaa cttagccgta caaggtttag cacagatcgt
    38821 tatatggata actgtccatt tagtttattg attgaagcaa caaaatcttt attctcagaa
    38881 tagataggag gggaaaattt tggatgcaaa tgtataccag cactttcggg cagatgaacg
    38941 tacatttatc gattcggtag gcgattggat tgaacaggtt caaggacaat atgcacccta
    39001 tctgaccgaa tttcttgacc caagacaatc ctatatttta gaaacactga ttcgacaaag
    39061 cagtgatttg cgtttcatgt tttatggcgg atatgaacag gcagaaagaa aaagatgttt
    39121 gattttcccc gattattacg aacctgtaga ggaagatttt gaaacagcat tgtatgaaat
    39181 ccattatcct agcaaatttg ccagcctaag tcatgggaaa atccttggaa ctttagtggg
    39241 aacggggatc aaacgctctt actttggcga tatcatctcg gacggtgatc gctggcaagt
    39301 atttattgca aatgaggtgg atcattttgt tgctgcacaa gtaacaaaga ttggaaaggt
    39361 tgctgtacgc ttggaaccaa tcaaatacac agaaatgatt atgccgaaag atagctggac
    39421 acaagaaaga ggaacagtta gttccatgcg actagacagt gtgatctctg agatttataa
    39481 tatttccaga cagcggtcca agcagctgat cgaagctggt aaagtaaaag tgaactggac
    39541 agaaaataca agacctgatt ttgtacttga actattagat attatttcag tcagaggtta
    39601 tggcagagtc cagatccaag aacttgaggg gaagacgaaa aaagaaaagt acagggtact
    39661 gttcggtgta ttacgtaaat aaaataaaaa ggtggtttac tcatggcttt aactccatta
    39721 gatatccaaa ataagacatt cccgacaaaa atgcggggat ataatcaaga cgaagtagat
    39781 gatttcttag atttagttgt tcgtgactat gaggaattga ctcaaagaaa tcgagaatta
    39841 gaaaaagcag tcaaacattc tgaagaaaaa cttgagtact tcaatgaatt aaaagatgct
    39901 ttgaaccaat caatcatcgt agctcaagat acggctgata aagtaaaaac aagtgcaagc
    39961 aaggaatccg aagtgatcgt tacttctgca caaaacaaag cagatgagtt agttgcgaac
    40021 gctgaaaaac gtgcacatca attaacaact gatgcagaag aaaaagcaag aaaaatcttg
    40081 acagatgcaa cagaaaaagc aagacagctt gcgacagaaa ctgaagatct taagaaaaaa
    40141 acacgagtat tccatcaacg tatcagcttg atgcttgagt ctcaattaga acaagtgaaa
    40201 agtccggaat gggatgaaat tttacaacca ttctccagct acgtgactga cagccatgaa
    40261 gtgatcaaag aagttttagc aaaacagctt gacaatgaaa atgatactga tgtaaactca
    40321 gacacaggag tcatagaagc acctatgacg gcatttgata cacaggctat tgatatctcg
    40381 atcgtgcctg gtgaaaatga aaacgagtag aacagaaaag cactttgtta ttcttaaagc
    40441 gagccgatga gagtggaagt tcggtaagac actgcatggt gaaagatcct ctacttgaga
    40501 cctgcttttc cgaaattaga gtaagaagca gcggctgacc tcgttattgg tcacagagag
    40561 tttgcactag atagatgcaa ctaaatttgg gtggtaccac ggcatgttcg tcccttgcgg
    40621 ctgaacatgc ttttttgtgt ccggaaaaca aataaaggag tgtatggttg aatgaaaatg
    40681 aaagaaacct tgcaattagg aaaaacaaaa ttccctatgc gtggtaattt gccaaaccga
    40741 gaaggcgagt ggcaaaaaga atgggaagaa aatcgaatct accaaaaaag acaagagttg
    40801 aacgaaggaa aacctagctt tgttcttcat gatggacctc catatgccaa tggtaatatc
    40861 catttaggac attcattgaa taaaatcagt aaagacatta tcattcgttc aaaatcaatg
    40921 tctggctatc gtgcaccata tgttccagga tgggatacac atggattgcc gattgaacaa
    40981 gtattggcta ataaaggcgt gaaacgtaaa gaaatgtcca tggcagaata cttggaaaaa
    41041 tgtcgtgaat atgcactttc ccaagtagat aagcaacgag cagatttcaa acgcctagga
    41101 gttgcaggag attgggaaca tccttatgtt acattagatc cttcttatga agctgcacaa
    41161 gtacgcgtat ttggtaaaat ggctgaaaaa agctatattt acaaaggatt gaaaccaatt
    41221 tactggtctc cgtcaagtga gtcttcttta gcggaagctg aaattgaata taaagatgta
    41281 aaatctccat ctatttttgt agctttcaat gtcaaagacg gcaaagatat tcttgatgaa
    41341 gatacagctt ttgtcatttg gacgacaaca ccatggacat tgccagcaaa ccaaggtatt
    41401 gctgtaaatc caaaatttac gtatgtattg gtggaagccg atggccgtaa gtttgtcgta
    41461 gcgaaagatt tgctagaaac agtccaatca gaaatcggtt gggaaaatgt aacagttcta
    41521 aaagaatttg ctggacaaga aatggaatat atgactgcaa gtcatccatt ctatgaacgt
    41581 gaatcacttg tgattttagg cgatcatgtt actttagacg ctggtactgg attagtccat
    41641 actgctccag gacatgggga agatgactat atcgctggta gcaaatataa actatctgta
    41701 gtgtcacctg tagactcaaa aggattgttt acagaagaag cacctggatt tgaaggtatc
    41761 ttttatgaca aagcaaatcc tatgatcact gatctattaa aagaaaaagg agctttgctg
    41821 aagctagatt tctttactca tagttatcct cacgattggc ggacgaaaaa gcctgtgatc
    41881 tatcgggcaa ctccacaatg gtttgcatct atcgataaat tccgtcagaa tattttggat
    41941 gaagtagaaa aagtagagtg ggttatccca tggggtaaaa cacgtttgta taatatgatc
    42001 cgtgaccgcg gcgattgggt aatttcccgt cagcgtgcat ggggagtacc acttccgatt
    42061 ttctatgctg aaaatggaga agcaatcatc acaccagaaa caatcgaaca tgtggcaaaa
    42121 ttatttgctg aacatggatc aattatctgg tttgaaaaag aagcgaaaga acttcttcct
    42181 gaaggattta cccacccagg ttctcctaat ggcgaattta caaaagaaaa agatatcatg
    42241 gatgtctggt ttgattctgg ttcttctcat gaagctgttt tacgtcagcg accagaatta
    42301 tcattcccag cagatatgta tttagaagga tcagaccaat atcgtggctg gttcaactca
    42361 agtatcacga ctagtgtggc tatcaatggt gtagcaccat acaaatcagt tatttctcaa
    42421 ggatttgctt tagatggtga aggccgtaag atgagtaaat ctttaggaaa catcattgca
    42481 ccagaaaaag tgatcaaaca atttggtgca gatatccttc gcttatgggt atctagtgta
    42541 gattatgaag cagatgttcg tgtttctatg gatatcttaa gtcaagtggc agaagtatat
    42601 cgtaaaatca gaaatacgat gcgtttctta ttagcgaata cagaagattt cgatcctaaa
    42661 aaagatactg tttcgtacaa tgatttacgt tctgtagaca aatatatgac cgttcgattg
    42721 aaccaagtaa tcaaagaaat ccgagaagaa gggtacgaca aatataactt catgcatatt
    42781 taccgtacag ttatgaactt cttgacggtt gatctatcgt cattctacct ggattttgct
    42841 aaagatgtag tttatattga agcagaagat gattatcaac gtcgttgcat gcaaactgtt
    42901 ttctatcaaa cagctgtagc tttgacaaaa ttattgactc ctatcattcc gcatacagca
    42961 gaagaaatct ggtcattcct gaaagaagaa gaggagtacg ttcagttaag cgagtttcct
    43021 ggttatgaag agtttgcaaa ccaagaagaa ttgatggata cctggactgc tttcatggac
    43081 ttccgtgata atgtcttaaa agcattggaa gaagcaagaa acagcaaatt gattggtaaa
    43141 tctttggaag caaaactaac tgtttatcca aaacaacaag ttcgtgaaat gttaaaagct
    43201 ttggatgcag atattgcaca attattgatc gtttcaccag actactttga agttatggca
    43261 gcagatgaac aagtgccaga taacgctatg gtatttgatg atgttgctat tacagtggaa
    43321 aaagcagacg gggaaacttg cgatcgctgt cgccaagttc gtaaagatgt aggagtagat
    43381 gaaaaacttc ctcatctatg cggtcgttgt gcaaaaatcg ttgaagactt ctatcctgaa
    43441 gctgtagctg aaggattcga agaaaagtaa aagggaaaag ggggtgtgac atgttctttg
    43501 tcacaccccc ttttgttatc aatcgtttaa ttttccacgt tcacgatacc agttatctgg
    43561 ttgccagatc cattcgaatc cgtctttttc aagtagttcg aaagctgctt taggtcccat
    43621 tgatccagct ttgtagttgg gaaaatccgg ttgtgtttgg tcccaagctt ttcgaatgac
    43681 atccacgata tgccaagatt gagcgacttc ttcccaatga gagaagttcg ttccatcacc
    43741 attcaacgca tctaatagta atttttcgta agcttctggg ctattttcaa ccatttctgc
    43801 actatttcta aattccaatt tgattggttc cgtttcaaat ccttgcccta cttctttccc
    43861 attcaaggat aaagaaaatc cttcagttgg ctgaatatag atcgttagaa cattaggtgg
    43921 aagtgtcgta tcgtcacatg gttcatcgac agatgttttg aatacattaa caggaacttg
    43981 tttgaatacg atgttgatcc gagtcccttt ttcagtcaaa cgtttgcctg tacgtacata
    44041 gaaaggaaca cctgaccaac ggaagttatc gatagtgaat ttgcctgcga cgaatgtttc
    44101 agtctctgat tgagcatcca ctttgtcttc ttcgcgatag cccttgaatt gtacaccatc
    44161 caattcacct tgagcatatt gcccgcgaac gaagttttct aacacttctt cttcgttata
    44221 aatgcggata gctttcaatg ctttgatctt ttctgtacgg atttcttttt cggaaaatgc
    44281 tactggcggt tccattgcaa gtaatgctac aacctgtaaa atatgatttt gcaccatgtc
    44341 tttaagtgcg ccactatgat cgtagtagcc cccgcgatct tctacaccga ggttttcgcc
    44401 aaaagtaatt tggatattgt cgatatagcg attgttccat tgagcctcaa aaatattatt
    44461 ggcaaaacgg atagctgaga tgttttggat catttctttt cctaaataat gatcaatcct
    44521 gaaaatatct tgttctggga atacttctct gatttgctgg ttcaattcaa aagcagactg
    44581 gtaatcagaa ccgaacggtt tttctatgat caaacgattg aagccatttt ttgtcataat
    44641 attttgtgat ttcaaatgag aaacgatcgt tccaaaaaat tgaggtgcca tagccaaata
    44701 atagacacgg ttaccgttta attgatagtg tccatctaat cggtcagaca attcttttaa
    44761 tgtctgataa tgcgttgaat catttacatc atgggattga tagtaaaaat ggcttgcaaa
    44821 ggtagttgct tcttcatctg aaggattcaa tgcacggatt gtttctttga caacttcacg
    44881 gtagtgctca tcgctccagg gacggcgagc ggttccgatt acagcaaaat gctcgcctaa
    44941 atttcctttt ctgtaaaggc ggaacaaaga cgggtacaat ttacgctggg ctaggtcacc
    45001 tgtaccgccg aatatcgtaa acaatacatt tttttcatta gacatgtgga tacactcctt
    45061 tatattttat cgacaaaaat cgtgctggcc gccttaaagc tgacggggat tttctttcta
    45121 tcattttgga tagtgatcgg tccttcataa gctgcgatgt caacgatctc atattcttcg
    45181 ttgatattca ggtcaatagt gactaagtaa tccaacaatt ctttttcatc aagcactcgt
    45241 gcgatacgca cttttgtacc gacaggatag gactccaatg tttgtcggcg ttcttcatgg
    45301 acaacttcct cttttttagg aatcgatcct ccatgaggac aatgttcagg atgattcaaa
    45361 tagttttcta aatgatctgc aagatgttcc gaagtcacat gttccaatac ttcagcatca
    45421 tcatggactt cattccagct atagtctaaa tgttcaacta aaaaaacttc ccataagcga
    45481 tgtttgcgaa tcagactgct agctttttgc agtccgcttg cagtcaattg tacaccttga
    45541 taaggggaat gttctacaag ctcttctttc acaagtttag aaatcatttc actgacagat
    45601 gcagctgaga catctagact agatacgatt tgtttattgt tgactttcgt tgtatctcca
    45661 cctagttcta aaatgatttt caaataatct tctcgatttg gtgtcatatt cgatcatcct
    45721 ttcatggtaa aaaaccatca ttagtaagtg taacaaaaaa aacgttaatt gactattttt
    45781 ctaaataaaa aacaaaggat ttttagacgt ttttacaaca aaaaacgaaa gatgatagag
    45841 aagactcatt ctttcgttta taaaggatat atatatacat tgggagggat ctatttttca
    45901 gataacaact tatatgaagt gtctgcagat tcgtcataaa gagtttgttc gggataagaa
    45961 aagataagat aaaaaccata ctgatcacgt acgactaaac gatattcatc tatttctacc
    46021 acagatgtgc caagagactg accgttgatc aaaaacgtgt aatcagatgt aatctgtaat
    46081 tgttgttttt gctggctatc actaaagaac catgtacctt gatactttga gagtccttct
    46141 gacttttccg cttgtttttt ctttttcaag taaataaaac ttgagagaac ggtaatgagt
    46201 gaaccaagaa aatagatcat tttttttatc accaaatcac tcctttatga gaaaataatc
    46261 gcatagacat attctttctt ctttatttca ttgtaccgaa taaaagctaa aatttctcta
    46321 agactttcga ctggtttttt tactgttttt ctacaaatta tgataggata catagtagga
    46381 agaaaggtga cataatttga agtatcagca agtggaaata gcttttttta ttgaccgccc
    46441 gaatcggttt atagcacatt gtatgaaaaa agacggagaa ataattattg cgcatgtcaa
    46501 aaatacaggt agaggaaaag aggtcctgat accaggcgct gaagtggcta ttgtatttgc
    46561 tcctggacct aaacggaaaa ccgcctatga tctgattgct gtaaaaaaga agggtgactg
    46621 gtttaatatc gatagtcaat tgcctaatcg gctggcaatt gatggtattt tagacggcac
    46681 gatcctttta ccaggattaa ctggtccatt tacggtatac aagcgtgaag taacttatct
    46741 tcactccaaa tttgatattt atttagagac ggtggtcgga caaaaagcat ttgttgaagt
    46801 caaaggtatg acacttgaaa ataaagcgat tggtgctttt cctgatgcgc ccacactcag
    46861 aggattgaaa catataggag aattaacgta tgccgcacaa gatggttatg ccgcttatgt
    46921 cttgtttatt gctcagttcg aacatcttca tctggcaacg atccatgaag aaatgcaacc
    46981 agcactagct gatatggttc gacatgcgca gcaaagcgga gttcaaatcc tcgcctataa
    47041 ttgtcaagta tcggcagatg aggtaactgt caagcaggcc attccatttg atgtagatat
    47101 cccttttgaa gatcctatac aatagaaaga aggtaaaata atgatcaact taggtattat
    47161 cggaacaaat tggattactc atcaatttgt ccaagcagct ttaagcacaa aaaaatatga
    47221 attgacagcg gtatattcta gacatttgga aaaagcacag gaatttggtt ctcattatga
    47281 aggggatatc gaattcgcaa cagatttgaa gacttttttc aatattactc atatggacac
    47341 catttatatt gcttctccga atagtcttca ctttagtcaa gctaagcaag caattttggc
    47401 aggaaaaaat gtgatagttg aaaagccagc tttttcgacg ccaaaagaaa tggatgaaat
    47461 catccatttg gcaaatgaac acaatgtgta tttttttgaa gcagcaagaa atatccatga
    47521 atcaggtttt aaaaagattg ccgattactt accattaaaa gacgagataa tcggtgcaaa
    47581 tttcagctat atgaaatatt cttcgcgcta tgaccaagta ttagatggag aagaaccgaa
    47641 tatcttctcg actcatttct caggtggtgc attgatggat ttaggtgttt accttgtata
    47701 tgcagctgtt gcttggtttg gaatgccaaa agaagtccat tatttcccaa gaaagatttc
    47761 tactggtgta gatggtcttg gatgggggat cttacgttat gagaattttg atgtatcgat
    47821 ccagccaggt aaaatcggtg attcttattt accgtcagag atttattttg actcaggaac
    47881 gctagtgatg aatggagtca atgcaattga acaagcacaa tttcatgatc gcgcgcataa
    47941 agaaatcatt gatttggcgg taacagctgc agaaaatcca atggcagatg aagcagagga
    48001 ttttgccaat gtcatggctc atccgaaaga tccagcttgg ggattgttgt atgaagagtg
    48061 gatcgagcta gctagaaatg tgaaccaagt gatttatgat ttaagaaaaa atggcggcat
    48121 caaattcgat gcagataatg aggaagattt ttaatgaacg aatttgtaca agtatttccc
    48181 gaaaattggc agaaaaaata tgaggataaa gggtttatct caccttctct tattcaacga
    48241 gcagtttttc agccactaag taataaacaa agtatagtag cggtttctcc tacaggttca
    48301 ggtaaaacac tggcttatct ttggcctcta ctactaaacg tggaaccagg tgaagcaagt
    48361 gcgttggtca tttttgcttc ttctcaagaa ctagctattc aagtagcaga tgttgcacgt
    48421 gaatggggaa aagacaaaga gttgaaagtc caatctttgg tgggcggagc caacgtcaaa
    48481 cgtcagattg agggtttgaa ggaaaaacca gaaatcttga ttggtacacc aggaagaata
    48541 ttggaattga tgaaagcaaa aaaaataaaa gcacatcaag tgaaaacaat ggtatttgat
    48601 gaagcagacc agctatttga cgctgggaac agccaaatca ttgatcaaat tcttcatcaa
    48661 gctccaacag agtatcagct agcttttttt tcagcgacag cagatcgttc ccttgaaagc
    48721 attgaaaaaa tcactgggaa aacgtttgag acaattgacg tgacagcaga ggacgattct
    48781 cgaaaaggac tgagacatta ctttttacgt gtaccgatac gaaagaaaga tgactacttg
    48841 agacgtctca cgcatataga agattttcat ggtctgattt tctttaatca actgactgaa
    48901 ttaggcgtaa tggaagaaaa attgctgtat agaggtgtac cggtaggaag tctggcctct
    48961 gatcagaaca agctactccg aaaagcagcg ctagaacaat tcaaaaagaa aatggtcaga
    49021 gcacttttga ccacagatgt ggcggctaga ggtttagata ttacaggtct tccttatgta
    49081 gtgaatgcag aagttccgtt aagtgaagaa gcttatttgc atcgttcggg aagaactggt
    49141 cgaatgggta atgaaggaat agtaattaca ctcgtacaag ataataatct aagagattta
    49201 aaacggcttt tacgtccatc aaatataaca cttgaagaga tttttttata cggaggtaag
    49261 ctgcaaactg agcagccaac caaagaagat gcagctcctg cagtaaataa aaagtatgcg
    49321 aaaacttttt ctggaaagga gcagaaccca gaatataaca aagaaaaaat aagaaaaaaa
    49381 gttaaaaata aatcaaaaaa agagagaaat aaaggagcaa ggcgcaaata agcgcctttt
    49441 tatgctatga ttatgaaaac gggaacaaaa ttggttgcca gaggaggggt tatgtgaaag
    49501 aacgtcaaga agagatcttg cagctattga cagaaaacca acaaggattg acagcaagtg
    49561 atgtagctga gcgcttaact atcgatcgaa gcaatgcaag ccgatattta agtgaactat
    49621 acaaggccca tcatattgta aaaaccgctg gccgaccagt cgtttactct ttgccaacgg
    49681 aaaagtcaaa atctgatgaa gtgcatgtcg actcttctac gcaagtgact tttgaaacac
    49741 tcgttggaga aaatgattct ttaaaagtca gcatacagca ggcaaaagct gcgattcttt
    49801 atccaccgcg ggggttgcat acgattattt ttggagaaac tggtacaggg aagtcgatgt
    49861 ttgctgaatg tatgtatcat tttgcaatcg attcagagat gctttctgca gatgcgccat
    49921 ttgtttcttt caattgtgcg gattatgctc aaaatccgca actgctcttt ggacacatct
    49981 ttgggatcaa aaaaggggct tacacaggag catctcaaga tagtcccggt ctaatagcca
    50041 aagcagacgg cggtatattg tttttagatg agatccaccg tttaccgccg gaagggcagg
    50101 aaatgctgtt tactttcatt gataaaggta tttaccgtcc gcttggagaa agtgcacaag
    50161 tacatgaagc atctgttcag atcattgggg cgacgactga atcttcagaa agtttcttat
    50221 ctacgttcaa tcgacggatt cctatggcaa tcaccttgcc gaatcttgct tcacgttcac
    50281 tggatgagcg ttatgaaatc atctctcttt ttatcaaaca agaagcgaat cgtttgaatc
    50341 aacgtatcga tatcgaaaaa gaagcgattc ttgccttcat gctttatgat gcggaaggaa
    50401 atatcggtca agtgaaacga gacttgaaac tagtctgtgc aaaatctttt ttacattacc
    50461 gtactcatca tgagaaaaag ctaatcattc gaaaagaaga actttccctt caagtacaaa
    50521 aaggattact gaaaatcaag gaaataccgg aacgtctcga ccgattcatg gacagcaaaa
    50581 gtcagtattt gacatttgaa ccaggatttg ctgatgttgt ctggtcacaa gatccagaac
    50641 gaaacatgca ggtctataac gatattgagg aaaaagtcac ttctctttcc gaaaccggtg
    50701 tggagaacat tgatctagag accctcattt caaaagatgt ggatgcttat ttccagacat
    50761 atgtcaaaga attgactaaa agttcgattc ccaaagatct tttgccagat gaaatctggc
    50821 ggttaaccaa tcaattgtat gatgtggctg aaaaacggtt agaccgtatt tataatgaaa
    50881 aagctcgttt tgcttttgct ttgcatctgc aaagtacatt ggatcgtgtg agagaaggtc
    50941 atatgatcgt ccacccagat ttgaataacg tacggaaaaa ttttaagagg gaattccagg
    51001 tagcaatcga tctttcaacg atcattgaag aagaagaaca agttgagatt ccttttgatg
    51061 aaatcggctt tattagtatg tttctttcta tcaatctagg agaaagtgag cctgttaaag
    51121 aaaacaaagt agatgttctc atattgatgc acggcagaca gactgcttca agcatgttgg
    51181 agatggcaca agaactatta ggaacaaaag tagggaaagc ttttaatatg ccactggtta
    51241 tggaagttca aacaatgtat gaagaagtgt tacaatatgt caaaaactat caagagcatt
    51301 tgacgaatgg cctactcttg ttaacagata tgggatcatt aaacaccttc agtgatttga
    51361 tttatgaaga gactggcatt cggacgaaag ctatcacgat gacaagtacc atgattgtga
    51421 tagaagcgat acggatggca agtgttggaa gaagcttaga agatatttat caaaatatcc
    51481 agctttcctt tgaatcgatc gtacgtgatc agttcaaagc ggaaaagtct gaaactttac
    51541 agcgtaaaaa agccgtaatc gtgacatgct ttactggtga aggagtagct gccaaattat
    51601 atcagcgtat tatgccagtg attaatcaaa ataaggtaga actgatccag atgcagtttt
    51661 tagaacgtga atcattcaaa aaacacatcg atgccttatt ggaagaatat gagatcaaag
    51721 caattgcggg aacagttgat gttgattatc aaaatatccc atttttttca gcctatgacg
    51781 tttttgatga tgaaaaacta aatattttaa agcgtatagc tgatgatgag atcccgacag
    51841 ctaaaattgt cgattcactt gctggatcat taagcgatgt cttttctatt gatgaactga
    51901 tccgttcatt gcaaaagaca gttcagcaga tccagaccga tttgtcgatt atcgtggaac
    51961 ctggagtaga tactggaatc gttatccatt tagccttcat gttagacgga ttgatcaaac
    52021 aagaaagcca tcggcaattt gagaaactgt ctattttctc aaaaatttat cgtttggaaa
    52081 tggatatcgt tcgggcaaac ttgatggcac tagaacgaaa atatcaggta aaaattccag
    52141 aagatgatat cgcatttctt acacaaatgt ttcttgaaaa taaaataaaa aaatgaacac
    52201 actaatcata ctgtttacac actaaacatc tgtgtgcgca ctataaaaaa gagctaaaaa
    52261 gcatgaaaac gctatttcag ctcttttttt tattggcatg cttcttgcgt tatataaaag
    52321 tgtaaaaaaa taaaaggagt gttaaaaatg gcaaaaaaaa caatcatgtt agtttgttca
    52381 gcagggatga gtacgagttt attggtcaca aaaatgcaaa aagcagcaga agaaaaaggg
    52441 atggaagccg atatttttgc ggtctctgcc tcagatgcag ataataattt agaatctaag
    52501 aatgtcgatg tattattatt aggtcctcaa gtacgtttca tgaaagctca atttgaacaa
    52561 aaacttgcgc ctaaaggtat tccattagat gtcatcaata tgcaagacta tggcatgatg
    52621 aacggagaaa aagttcttgc acaagcagaa aatctaatga aataaatcaa gtatgcagtt
    52681 gggggtataa caacgtggaa gatcaacaaa atctagaagc catcatgggg ttaatcatgt
    52741 ttggcggtaa tgcaaagagc gatgcaatgg aagcgattgc ggctgctaaa atcggtgatt
    52801 ttgagttagc ggataggaaa atcgctgatg cagaagaatc acttatacaa gcgcatcatg
    52861 cacaaacaga catgttgaca caagaagcac aaggaaatca tatgcaagtg acattattga
    52921 ctgttcacag tcaagatcac ttaatgacat ctattgcgtt tactgattta gcaaaagaaa
    52981 tcatcgaact ttatcgtcgc gtatacagcg aataaataca acttcttttt tgaacggaag
    53041 aagaaaaatt tttgaacgga ttttttattt tgttataagg agaactctct ttatataaaa
    53101 aacttatagg aggaagtaaa aaatgaacgg actatcaagt tggatggaga aatacatcct
    53161 gccggttgcc gcaaaaatcg gagcgcaaaa acatttagtt gctttgcgtg atgcttttat
    53221 cggcatactg cccgcaacaa tggcgggatc agtcgctgta atgatcaatg caattattcg
    53281 tgatttaccg caacagttta acagtaccta tgctgacagt ttagcagcag acaaaggaat
    53341 ctttgccgta ttgaatgtca tcatcggaat caatggtttt gtctggaacg gtacactagc
    53401 aatcgccgga ttgatctttg ccttttcttg ggggtataat cttgcccgag cttatggagt
    53461 aaatgatcta gctgggggga tcgttggttt agctaccttg attcaaggta tttcctttgg
    53521 tccagcatta tcagcagcat tagatgtgaa tgttccttca aatgtcgcag atgcaatcaa
    53581 tagttctgag attggtgcaa cgattgcaga tggcacactg tcagttggat tatggggttg
    53641 gctaaaacta gatcatttga atggaaacgc ttattttaca gttatgatca tgggagctat
    53701 ttcagtcatt attttttgta aattgatgct tgccaacatt acgatcaaaa tgcctgattc
    53761 agtaccacca gcagtatcta aagcatttgc agccatttta ccagcgacga tcgctttata
    53821 cgtcattgct attatcaatt atatcgtagg tcgtgtagct gatgggcaac tgatcattga
    53881 tttggtgcaa aaatatattg cagaaccatt cctaggatta tctcaagggt tgggagcagt
    53941 attgatcgtc acagtatttg ttcaagtatt ctggttcttt ggtattcatg gatcaaatgt
    54001 attggcacca gtgctagagg gaatctgggg acaagcacaa ctaatcaata ttgatatttt
    54061 ccaaaaaggt tataacggaa aaactggtac accagccgtg ttagatgcaa tcgaagatgg
    54121 aaaagcttat atgtgggtcc gcggatcgtt cgatgctttt gcatggtttg gcggatcagg
    54181 cggaacgatc gtcttactga tagctatctt gattttctct aaacgtgcag attatctaac
    54241 agtagctaaa ttatccattg ggcctggtat cttcaatatc aacgaaccag tgatgtttgg
    54301 tctgccaatc gtattgaatg caatcatgtt tatcccattc ttgattgctc cagtagtagc
    54361 aacagcaatt ggatggacag ctacttatct tggcctagtc gctccggtat cgcagcaagt
    54421 cacatgggta gtaccgccgt tcttattgtc attcttagca acaggagcgg attggcgggc
    54481 accgatcgta acattagtat gtatggtcgt gactttcttg atttgggcac catttgtcat
    54541 ctctgcaaac aaaatggacc ctgccgcagg cgaacaacaa taagatgaac gtgaggagcg
    54601 ggacgcttta agtttcgttc ctcataatga attttcgagg aggaactgaa atgattacaa
    54661 ttgccagtgc aatgcaacaa gatgaaatca aagaattgtt agaaggatac aatgaagaag
    54721 gaatgacttt tacatttaaa gaaaaacaag gaatcaagct gttctttgaa acaagtgcag
    54781 aagatccgga agttgcggct aaaaaagcaa aggaactaat caaagcacag ccttggggaa
    54841 cagtccttta ttttcaatca acagctgttt agaaaatggt ttaaaaagat aggatggttg
    54901 agatgataaa aaacggttta tttttatgta gtattggttc tttgatcgtg ttgtatagtt
    54961 tgaatccaca ttcgcaaagg tatgatttgt atagtatggt cacaggtctt tttctaacag
    55021 gattaggaat ctatttcttc atgaagggga aaaaaagaga agaagcaaag gagaaaaata
    55081 actaatggaa atcacgaaaa aattaaatat tccagcatcg tttttttacg ataaagttat
    55141 ggactctgtc cttttcgatg tacgtaaagc gaccggcaaa tcagtaacta gaaaacagct
    55201 gaaaaatttg gagtacgtca aacaattctc gcaaaataac cgggctcgca taaaaatcga
    55261 agaagtcatt gaaaaccagt cttataagtt tcgtacatcg acaacgaaga atgaattcgt
    55321 cgttcattat caaatcaaac cattggacga ttcgacctgt gaggttcatt acacagaaaa
    55381 aatggagtct tatggcatgc ttcaaaagtt gaatgatatg cttttaggaa caatcttgat
    55441 gtattttaaa cggagacgtt tcaaaaaaat gctagatatg atcgaagcaa cgtattaaat
    55501 caatgaaaca tggtactatt tagttattga aaaatgaagt aagctagaag gaatttttca
    55561 taggaagata gatctttcct gcgaatcctc actagctttt ttgtaggaag gggcagcaaa
    55621 atgacaaaca aagtaagaga gctaggtgta tcttcgattg aggaaatgtt tgatttagcc
    55681 acttatgcat ttaatggggc agatacacct gaaaggcgag aacgttttcg tacacttgcg
    55741 gagaattctt ggaattacgg attttttgat gaggaggatc ggctaacaag tcaagtcatg
    55801 gcgacccctt ttccagtgaa tttttacgga caagagtacc taatggccgg cattggctat
    55861 gttgcttctt atccagaagc aaggggacaa ggcgggatca atcagatcat ggaaaagatc
    55921 cttgaagatt gccgtgatcg taaagtaagc ttatcttatc tggcgccatt ctcctatccc
    55981 ttctatcggc gctatggcta tgaacaatca tttgataaaa tcagttatca actaaacagt
    56041 cgtgatgtgc cgtttatcaa aaaaaaatct ggaaaaataa agcgtatgga ttttgaagat
    56101 gcaaaagctg caatcagaag aatttatgaa cagatgccgg aaaaccaacg agctggtctg
    56161 aagcgcgaag actggtggta tacgtataaa ttcaagcaaa aaggcaatta ccagtttgct
    56221 ttgcattttg atgataatga ggaagcaacg ggatatatcg tttatcagct tgcaacgtct
    56281 tcctttatta ttgcggagtg gggatttttg gatcatgatg ctttttgcag ccttgttcgc
    56341 ttcgtaagtt ctcacaatgg tgcttttgaa acatttgaat attcatgcgg ctacggcggt
    56401 aatgatcaaa attatctatt agaaaatcca tttgcttctg taatgatcac accttatatg
    56461 atggcgagga tcgtagatat ttctttgttt ttggaaaatt atccatttaa aaagagacaa
    56521 gctgcatttg ccctacaaat agaagaagat cattatgcga aatggaatga aggaatcgtg
    56581 gaagtaacaa tcgaaaatgg aaaaaatcat ctctcaaaag tagaacaaac aaaactccca
    56641 atcgttcgag gaagtatcca acatatcacg cagctattat tagggtatcg taaagtggaa
    56701 gagttggtat tccaagaaag aatacaggtg acaaaagaat atgaagaaac gttaagctca
    56761 atcttgccac agcaacagcc aattcttaat gactattttt gataaaaaac gggtgatgac
    56821 aatatttttg tcaccaccct tttgtttttc atttggcatt tgaaggaaaa tgttaaagta
    56881 aatcctcttt tttcgtaaat ttgaattttt tgttttcttt tgttttaggg ttttcttttt
    56941 tgtggtcttg gattgcttct ttcgtaatct tttcccgatt ttttttagat gaatctaagc
    57001 gttctgcgta tactttttct tttggcttat cattcattgg aaaggacctc cctacattat
    57061 ttcattctac ctctagttta tcatctgtta aggactagtg gaaacgtttt gcctaactta
    57121 taagctgatt tttttagcag ataagcttgc aagtaaaaaa caaacaagct acaattgatt
    57181 tattgaaatt gttcctgtag ctcagtagga tagagcaatc gcctcctaag cgataggtcg
    57241 ctggttcaat tccagtcagg aacgtataaa taacccctgt atcctttgcg gtacaggggt
    57301 ttgttttgta tttgtgccaa aaatgtgcca aactagttat catttttata taaactatcg
    57361 aacacagtat tgattttttc tttatcctga tcttcaagct ctcgaacaat atgaagataa
    57421 gtagacattg tagtttctaa actgctatgt cctaaacgtt tcgatacgct aagtatattt
    57481 actccttgat aaagtaaaat agatgcatgt gtatgcctta gtccatgcaa agtaaattgc
    57541 ttttcaatcc ctaattcttt taattttttg cgcaaatatt tgctgacggc gtttgatgaa
    57601 actaatccat tttttaagtt gaaaaaaaca aaattatgag ggtttttgat ttcaaaattc
    57661 tcgaataatt ctttttgatt gattttaaac ttttttaaga gatcaatcgt atgtttatca
    57721 atggaaattt tcctatttga agtttcgttt tttgtttttc cccattcatt taatttataa
    57781 ttccatgttt tattaatagt gatgatttga tcttcgaaat cgatatcttc ccatgttaat
    57841 cctagaagtt cagcaaatcg cattccagtg gcaccagcaa ctaacacgag cataggagaa
    57901 gagtactgcg cagaaaggtt ttcttctacg agtttcatta aagatttaaa ttgatcataa
    57961 tctaaatatt tatcttcttt tggcttcaat gaatcttttc ctttgattac tgcttttcta
    58021 gttggatcaa acggaattaa tccttcttct acggcatctt tcaacgacgc tctaatatgg
    58081 ttattaaact taataacggt agattttaca tggtctttag catacttatt taaaaagcgt
    58141 tgatatccca ctctatctaa atcagaaatc aataccgctg gcatatattt ctttatgttc
    58201 attaacgtat cttcgtattt tctataagtg ataggagata ctgttccttc tttataaagt
    58261 tgcatccaat cctcaaagta atctgataat aatagatttt tgcgatcccc tttaaggcct
    58321 ttgttcaagt tatattctaa ttcgttggcg gcatcttttg cttctccttt tgtcttaaat
    58381 cctgattttc ttatcttcgt gtattttccg tcatcttttt tgtatgaaat ttcgtattgc
    58441 caactatttc cacgtttgac taatcttgcc ataattgaat cagctctctt tctctgatac
    58501 aataggaact ataaagaagc ccattgtttg ggttttatta ttttttgcac atcctttttg
    58561 cttgccggcg caaggatgtg ctatttgtcg ttttaatggt ataattggat gtgtattaga
    58621 tcatccaatc agaggtgttt attatgatta aagtgatttt taaaagaata actgaacttc
    58681 cagagtttag attatttgtt gattattggt atgtctggtt ttttattgtt gcattgctga
    58741 taggtctaga tgtcatatta gaaagaaaac ttaagtaaga gttgagttaa taaattgaat
    58801 tcctaaatga gatattttgt agcccatatt ttgtttgccg acatcaccta atactatgga
    58861 gctaatcgtt ttttctaaca gaccaaaatc agataatcta tttaagctac catctattag
    58921 ctcttgtgaa tagccagaca tgctgatatt tccagtgaaa ttttcgtttc cacgactcat
    58981 taagcatgct aacaattgaa tctcaatcga tgttaattga ttcagtacaa gaagataata
    59041 ctcctcgtta tcccatgaag cgttgttaat tcgaagtaaa caattcttat aaagattttt
    59101 atacatatct attttatttt gtgcctttga tttttctacc tcgtcattta tcccttcgaa
    59161 tattccaatg agttggtcct tgttctctaa gttgaaaaca tcttctggaa tttgatcgcg
    59221 aatgttttct aatgattcgt ttaaattttt gtagaatttc tcgatgcgtt tgaaacgctt
    59281 ctcgttttta gacccaaaat aaagtgactg cacaacgcca ccaatataag gaattgctcc
    59341 aataccgcct tcaactatta attcaagata ttcttttttg gttagttttt cattgctatt
    59401 atccatagtt acctcctatt ttgtcatttc ctttacaagc attgtgactc ctgatggcac
    59461 tcttttgtat aggttttatt attgtgcaca atctaactct tagtggggcg tggttcattt
    59521 attttttact tactttacta ttaatcatat cccattgagc ataggatttt tctatgttcg
    59581 atcctcttga actaatttct cctgtaaaag ttactttatc gccctttgta aaggtatcag
    59641 cattgtttaa gtccttcgca aaaattacat atgaaacttt aggtgttgca gaaatatcat
    59701 tccaactttt atcatcgtac tctttgtctg ctataatggc tattctagta gtcattgatt
    59761 cgataacagt tccttcaaaa gtatatgttt taccactaat atatttagta aaagctgtat
    59821 tttgctcagt agctggagta atagaataat aactatctaa gaaagcaccc cagtcatgat
    59881 tatttgtctc taaaaattct tcaaattgag ttaggctttt attgctgctg ttagatgtag
    59941 tagtttgagt atcagatgat gagctttcct gtttgttatt gtcatttgcc tcgtttgatg
    60001 aggaaagcga gaaacctacg gtagtaaaaa taatcccgat aataaggaga atcataggtg
    60061 ttttcttatt tgtttttttg ataaatctta caattaacat gattaaacct ataataaata
    60121 aaacaatacc agtaaaacca aatataccgt ccattaata
//
LOCUS       DACEXQ010000002        41654 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.002, whole genome shotgun sequence.
ACCESSION   DACEXQ010000002 DACEXQ010000000
VERSION     DACEXQ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41654
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.002"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..848)
                     /locus_tag="IXH04_000065"
     CDS             complement(<1..848)
                     /locus_tag="IXH04_000065"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAQ1850709.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMP"
     gene            1050..1487
                     /gene="zapA"
                     /locus_tag="IXH04_000066"
     CDS             1050..1487
                     /gene="zapA"
                     /locus_tag="IXH04_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAQ1850710.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            1545..2093
                     /locus_tag="IXH04_000067"
     CDS             1545..2093
                     /locus_tag="IXH04_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAQ1850711.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            2208..4568
                     /locus_tag="IXH04_000068"
     CDS             2208..4568
                     /locus_tag="IXH04_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAQ1850712.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            4655..4969
                     /gene="trxA"
                     /locus_tag="IXH04_000069"
     CDS             4655..4969
                     /gene="trxA"
                     /locus_tag="IXH04_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAQ1850713.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            5151..6929
                     /gene="uvrC"
                     /locus_tag="IXH04_000070"
     CDS             5151..6929
                     /gene="uvrC"
                     /locus_tag="IXH04_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAQ1850714.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            7074..7973
                     /locus_tag="IXH04_000071"
     CDS             7074..7973
                     /locus_tag="IXH04_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1850715.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            7966..9228
                     /locus_tag="IXH04_000072"
     CDS             7966..9228
                     /locus_tag="IXH04_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1850716.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            9391..11289
                     /locus_tag="IXH04_000073"
     CDS             9391..11289
                     /locus_tag="IXH04_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAQ1850717.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(11588..12307)
                     /locus_tag="IXH04_000074"
                     /pseudo
     CDS             complement(11588..12307)
                     /locus_tag="IXH04_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
     gene            12644..12976
                     /locus_tag="IXH04_000075"
     CDS             12644..12976
                     /locus_tag="IXH04_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1850718.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            12963..14966
                     /locus_tag="IXH04_000076"
     CDS             12963..14966
                     /locus_tag="IXH04_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAQ1850719.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKHQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            14985..15455
                     /locus_tag="IXH04_000077"
     CDS             14985..15455
                     /locus_tag="IXH04_000077"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAQ1850720.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            15476..16060
                     /locus_tag="IXH04_000078"
     CDS             15476..16060
                     /locus_tag="IXH04_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAQ1850721.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            16075..17061
                     /locus_tag="IXH04_000079"
     CDS             16075..17061
                     /locus_tag="IXH04_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAQ1850722.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            17051..17362
                     /locus_tag="IXH04_000080"
     CDS             17051..17362
                     /locus_tag="IXH04_000080"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAQ1850723.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            17384..19165
                     /locus_tag="IXH04_000081"
     CDS             17384..19165
                     /locus_tag="IXH04_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAQ1850724.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            19158..20534
                     /locus_tag="IXH04_000082"
     CDS             19158..20534
                     /locus_tag="IXH04_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAQ1850725.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            20541..21176
                     /locus_tag="IXH04_000083"
     CDS             20541..21176
                     /locus_tag="IXH04_000083"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAQ1850726.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            21206..22561
                     /locus_tag="IXH04_000084"
     CDS             21206..22561
                     /locus_tag="IXH04_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAQ1850727.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(22753..24147)
                     /locus_tag="IXH04_000085"
     CDS             complement(22753..24147)
                     /locus_tag="IXH04_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ1850728.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFNSPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            24239..25210
                     /locus_tag="IXH04_000086"
     CDS             24239..25210
                     /locus_tag="IXH04_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAQ1850729.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            25207..25989
                     /locus_tag="IXH04_000087"
                     /pseudo
     CDS             25207..25989
                     /locus_tag="IXH04_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
     gene            complement(26158..26529)
                     /locus_tag="IXH04_000088"
     CDS             complement(26158..26529)
                     /locus_tag="IXH04_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ1850730.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            26663..27106
                     /locus_tag="IXH04_000089"
     CDS             26663..27106
                     /locus_tag="IXH04_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ1850731.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(27149..27958)
                     /gene="yidA"
                     /locus_tag="IXH04_000090"
     CDS             complement(27149..27958)
                     /gene="yidA"
                     /locus_tag="IXH04_000090"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAQ1850732.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(27977..29344)
                     /locus_tag="IXH04_000091"
     CDS             complement(27977..29344)
                     /locus_tag="IXH04_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1850733.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(29328..30158)
                     /locus_tag="IXH04_000092"
     CDS             complement(29328..30158)
                     /locus_tag="IXH04_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAQ1850734.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            30321..30758
                     /locus_tag="IXH04_000093"
     CDS             30321..30758
                     /locus_tag="IXH04_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAQ1850735.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            30834..31454
                     /locus_tag="IXH04_000094"
     CDS             30834..31454
                     /locus_tag="IXH04_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAQ1850736.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            31643..32149
                     /locus_tag="IXH04_000095"
     CDS             31643..32149
                     /locus_tag="IXH04_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAQ1850737.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            32166..33065
                     /locus_tag="IXH04_000096"
     CDS             32166..33065
                     /locus_tag="IXH04_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAQ1850738.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            33152..34036
                     /locus_tag="IXH04_000097"
     CDS             33152..34036
                     /locus_tag="IXH04_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAQ1850739.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(34228..34299)
                     /locus_tag="IXH04_000098"
     tRNA            complement(34228..34299)
                     /locus_tag="IXH04_000098"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34264..34266),aa:Arg,seq:ccg)
     gene            34359..35693
                     /gene="rpoN"
                     /locus_tag="IXH04_000099"
     CDS             34359..35693
                     /gene="rpoN"
                     /locus_tag="IXH04_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAQ1850740.1"
                     /translation="MKFEQNFSQKQQQTQKLTMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            35896..36933
                     /locus_tag="IXH04_000100"
     CDS             35896..36933
                     /locus_tag="IXH04_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAQ1850741.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            36974..37975
                     /gene="gap"
                     /locus_tag="IXH04_000101"
     CDS             36974..37975
                     /gene="gap"
                     /locus_tag="IXH04_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ1850742.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            38085..39275
                     /locus_tag="IXH04_000102"
     CDS             38085..39275
                     /locus_tag="IXH04_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAQ1850743.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            39379..40134
                     /locus_tag="IXH04_000103"
     CDS             39379..40134
                     /locus_tag="IXH04_000103"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAQ1850744.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            40250..41548
                     /gene="eno"
                     /locus_tag="IXH04_000104"
     CDS             40250..41548
                     /gene="eno"
                     /locus_tag="IXH04_000104"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAQ1850745.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT        13783 a         7284 c         8891 g        11696 t
ORIGIN      
        1 ggcatgccgc ctttctccaa tagttttgcc gctacttggt cagatttcgt tccagctcct
       61 gatggaaggg tgattccagc ttcagctgat gctttgtgta actcttccaa aaagctggca
      121 cgactgatga tagaagcagc agcaacagct aaatgatatt gttctccttt agtaacgaag
      181 tacagttttt cttgtaattg atttttttct aaacgcgcat attttttaaa atttgcttcc
      241 ggcgtaaact gatcgatcaa aatcgcttct ggtttttctg gctcgatttt ttttagcagc
      301 aaatggatcg cttgattgtg caaagcgact ttcatccgaa cggcattgta ttttggttgg
      361 atctcattgt atttttctgg agataagaca agcagtttat aggggatggt tttttttaag
      421 atgtcagcaa gctggatgat ttggctatct tttaactctt ttgaatctct aacaccaagt
      481 ttacgtaatt ctgcaagctg atcacgtttc acataagcag cacatactgt tagcggacca
      541 aaatagctgc catttccgac ctcatcacta cctaaaacgg ataattgact gatatttttt
      601 ggcaaagaag aggctgtttt tgctggcttg gcttgtttat cctgtgcctc cccccattta
      661 tcagcttcta ttgctgcatt cgtaccttga aacatcactt ttccggatgt ataagcggta
      721 atggtcgtcc cgtctttttt tgcagcaaag ctagtataag gaatctgttt atttaacaaa
      781 taagagcgat agtatgcttt catttgacta agttggtcgt ttgttgcttt gataatgacg
      841 ttgctcataa aattctcctc gtttttatac ataaaactat accgcaactc acgacttttc
      901 gttttctaag agattgcgag acatagcatt tttttaaagt ttatcatata atagttaagt
      961 atgtgaaaaa tatttgtgga gaacggtgaa agaaccaact tcatggtaca ctaaataaaa
     1021 ctttcatcaa ataggaggaa atgattcgta tggcgcacga aaaaacacgc tacaaagcag
     1081 tgattgccaa ccaaacatat actattatcg gacgggaaac caagcatcat atggatatcg
     1141 taacgaagct gatcaatgaa cagttagcag aattgaaaca attatcacca caaatggata
     1201 atgaacaagc agcaatcttg atggctgtca atgcgctttc ggatcaattg aagaaacaag
     1261 aaagaatctt ggagctggaa gaagaaacag cagaattaaa gaaaaaaatg atcaaattta
     1321 cagagcttga aaatcgtgtg aaacgcattg aagcaatcga gaacgaagct cgggaagtat
     1381 tgaaagaaaa tggtcaagca gatcatacca ttaaaaatca tgtggaagca caacagattt
     1441 taaacgagaa acgaaaagga caaattaaac aaaaatcttc taattaagat ccttttatca
     1501 aaataagaaa aatttaaaaa agttagaaga gaaaggaacg tagcatgctg agtttgctga
     1561 ttttattttt attgctaatt gcgttttttt ctggagcacg tcgcgggttt gctttgcaaa
     1621 tcatattcgc gattggatat gtattgtcat ttattgctgc ccagcatttt tacaaaccat
     1681 tagcaagtca tttggaatta tacattccat acccagctgt caccccaaca tcaaagttag
     1741 cattttttga tcaggtattt gcttttcacc tagatgaagc tttttatgca ggaactgctt
     1801 ttctgattat tttgctaatt ggctggctgc ttacccgatt tgtgggggtt tttgtccatg
     1861 gattgacgta tgtgccgatc ttgagacagg tagactggat tgcaggaggc atcctgtcac
     1921 taatcatggc ttacgttact atatttttga ttttgcagtt acttgccttt gttccattag
     1981 atgtggtcca aaatcaattc ggcgagcata gtttcgctcg ttttatcgtt gagagaacgc
     2041 catttcttac caacaagatg tatgagcttt gggtaacgaa tattttgaat taaggaaaat
     2101 agtgctggaa aatatttcgt tttttgtaca gagtagttta tttaagaagg ctgcgtagcg
     2161 tatgtccggc acagctcttt tttcatagtg aaagataagg tgataaaatg aataaccgaa
     2221 tccttgaaac attggaattt gaaaaagtca aacagatggt cagacaattt gtagtgactg
     2281 cacaaggaaa agaagaactg gcagaattgg taccagtgag tgagaaacaa acgatcacaa
     2341 actggcttca agaaactgag gatggattga aagtgcaacg tctgcgaggc gggatcccta
     2401 tacctaaact tgaaaatatc cgtccccata tgaaacggat cgagatcgga gcagacttga
     2461 atggagtgga gcttgcacaa gtttccagag tattgtctac gacaagcgaa ttgaatcgtt
     2521 tcattgatga tctttctgac agtgaaatcg aatttgcacg tttgtatatg tgggcagatc
     2581 agttagtcac gatccctgtt ttgagccgcc gtttaaagga agcaatcgat gaagacggac
     2641 gcgtgactga tgacgcttct cctgagttaa agagcataag acagaacatt cggcgaagtg
     2701 aacaagctgt tcgtgaacag ttagatggga tcgtccgtgg gaaaaatgca aagtatttaa
     2761 gtgatgcgat catcaccatg cgaaatgacc gctacgttat cccagtcaaa caagaatacc
     2821 gcggtgtatt tggcggtgta gtccacgatc aaagtgcttc aggacagact ttatttattg
     2881 aaccaaaaca agtggtggat ttgaataatc gtttgcgtca ataccaaatc gctgaacgaa
     2941 atgagattca acgtatttta agtgaacttt ctgctgaact tgttccccat cgtcaagaaa
     3001 tcatccacaa tgcttatgtg attggtaaaa tggatctgat gaatgcaaaa gcacgatttg
     3061 gtaaagaagt aaaagccatc gtgccgggaa tcagtgaaga caatcatgtc gtcttgaaac
     3121 aagcaagaca tccactgatc gatcaggaaa aagtagtttc taatgacatc acaatcggaa
     3181 aagactatca agccatcgtc attacaggac ctaacacagg tgggaaaacg atcacgttga
     3241 agactttagg tctgttacaa ctgatggggc aagcaggatt accgattcct gcaggtgaag
     3301 aaagtcaaat cggtattttt gaggaagtat ttgcggatat aggagacgaa cagtcgattg
     3361 agcaaagtct atcgaccttt tcttctcata tgacgaacac tgtagatatt ttatcaaaag
     3421 tcaatgaaaa aagtctggta ttatttgacg agctcggtgc aggaacagat ccgcaagaag
     3481 gtgctgctct agcaatcgca atcttagatg atcttggtaa aaagtctgct tatgtgatgg
     3541 caacaacgca ttatcctgaa ttgaaagttt atggatacaa tcgggcaaat acgattaatg
     3601 ccagcatgga gttcgatgtg gatacactaa gtccaacgta tcgtttgctg attggcgttc
     3661 ctggacgaag caatgcgttt gaaatctcta gccgattagg tcttgataca gaagttattg
     3721 atgaagcgaa gcagttgatg aatgatgaga gccaagattt aaacgaaatg atcacagatt
     3781 tagagaatcg ccggaaaatg gcagagaccg aatatctgga aatgcgtcat tttgtttctg
     3841 aggcacaaga acttcacgat gatttgaaag aagcttacag ctatttcttt gaagaacgtg
     3901 aaaaagaaat ggaaaaagca aagaaaaaag caaacgaagt cgtgtcagag gcggaagaaa
     3961 aagcagaaaa aatcattgct gatattcgta aaatgcaaca gcagattggg caaggaaatg
     4021 tgaaagagca tcagttgatc gatgcaaaga cccaattagc aaatctacat caagaagaaa
     4081 ctttgaagaa aaataaagta ttgaagaagg cgaaagaaca aaaaacgttg aaaccgggag
     4141 acgaagtgtt ggttacgact tacggccaaa gagggacgct tttacgtaaa aacgggaatc
     4201 agtggcaagt agaaatcggt atattaaaaa tgaatgtgtc cgaagacgag ctgacgcctg
     4261 ttgctcctca aaaagagccg acacaacgag ttatccatgc tgtacgatct gaatctagta
     4321 gtcacgtgcc gaatcaattg gaccttcgag gaaaacgata cgaagaagcg ctaagtgagg
     4381 tagatcaata tttggattct gccattctag ctggttatcc tcaagtgact attgtccacg
     4441 gaaaaggaac aggcgcactt cgcaagggaa ttactgatta tcttaaaaac catcggagcg
     4501 tcaaaagctt tgagtttgca ccggcaaacc aaggcggaaa tggtgcgaca attgttaaat
     4561 ttaagtaaag cttactaaag gtaagcagat ggttcgctat ccaagtatag aaatgctata
     4621 ctaatcatag attttaaaaa tggaggtctt tgatatgtca caagtaatta cagataagac
     4681 atttaatgaa gaaacagata aaggacttgt attgattgat ttttgggcaa cttggtgtgg
     4741 tccatgtcgt atgcaagcac cgattttgga tcagttggaa caagaatatg atgaagaaga
     4801 attcagaatt gctaaaatgg atgtggatga aaatccagaa actcctcaac aattcggtat
     4861 catgagcatt ccaacattaa tgttgaaaaa agatggtcaa gtagtagaaa aagctgttgg
     4921 tgttcattca aaagaacaac tacgtcaaat gatcgatcaa tatttataaa aacttacagc
     4981 aaaaataagc tgagggtggg actgaatcca gttccgtcct cggttttttt gcggttgagt
     5041 taaggttatt caaattaata ttttttgagt ataattacgt tgtcaggctg gattgttttt
     5101 ttgacagaca tgctataata actttaattg aatatgaagg ggagtaccag atgaacgaac
     5161 gaatcaaaaa caaattagct ttacttcctg atcagcccgg ctgttacttg atgaaagata
     5221 aaaatggaac gatcatttat gtcggcaaag ccaaaatatt aaaaaatcgt gtccgctcat
     5281 attttactgg cagccataat acgaaaacgg aacggcttgt tagtgaaatc gaagactttg
     5341 aatacatcgt tacagaatcg aatatcgaag cattgctctt agagatcaat ctgataaaga
     5401 aaaatgatcc taaatacaac attatgttaa aggatgataa aacctatcct ttccttaaga
     5461 taacgaatga aaaatatccc cgtttggtga ttactcgtaa agtactaaaa gacaaagcac
     5521 tgtattttgg accatatccc gatgtcggtg cggcgaatga gacaaagaag atcctcgatc
     5581 gattatttcc tctgagaaaa tgcaaaccta gtcagacgaa ggagccttgt ctttactatc
     5641 atttgggaca atgtctgtgt ccttattatt tcgacgtaga tccgaaagtc tatacgaaaa
     5701 tcgttgatga agtgaaaaag tttttaaacg gaggacacga aaaaatcgag tcagagatcc
     5761 atcagaaaat ggaaaaagct gctgaaaaca tggagtttga aaaagctgca gaataccgag
     5821 accagatccg tgccattgag acaatcatga ctcgccaaaa aatgacaaat actgatttac
     5881 tggatcgaga tgtatttggt tatgcgattg acaaaggatg gatgtgtgtc caagtgtttt
     5941 tcgtaagaca aggaaagcta attgaacgag atgtttcaat gtttcctttc tataacgaag
     6001 cagaagaaga tttcttgact tacctcggtc agttttatca ggaaaatgag catttcattc
     6061 ctaaagaagt cttgattcct gatacgatcg acaagcccag tgtagaagct ttgttagcta
     6121 cgaaagtgct gcaaccaaaa cgaggagaaa agaaaaaatt agttgaattg gcgaataaga
     6181 atgcctcagt tgctttgaat gaacggtttg atctgattgc cagaaaacag gaacggacga
     6241 tcggagcagt tgaacgttta ggaaacgcga tgaatatccc gaccccaata cgtatcgagt
     6301 cttttgataa ctctaatatc atggggacga atcctgtggc tgcgatggtc gtattcattg
     6361 atggaaaacc ggcaagaaaa gaatatcgga aatataaaat aaaaacagtc aacggaccgg
     6421 atgattatgc atcaatgaga gaagtcatct atcgcagata ttcacgagtg attcgcgaag
     6481 ggttgccttt acctgatttg atcttgatcg atggaggaaa aggacaagta gatgtggcaa
     6541 aagatgtctt agaaaatcaa ttaggaatcg atattcctat tgctggcatg gcaaaaaacg
     6601 ataaacataa aacaaatgag ttgctattcg gtatagattt ggctgtagta ccgcttgagc
     6661 gaaattcacc agagtttttc ttactccagc gaatccaaga tgaagtccat cgattcgcaa
     6721 tcactttcca tcggcagcta cgcagtaaaa acagttttgc ttcccgatta gatgaaattg
     6781 aagggttagg accaaaacga aaaaaaatgc tgcttaaaga gttcaaatcg ttgaaaaata
     6841 tcacagcagc cacgacagaa gaattacaag aaatcggact gccgaaaaat gtcgcacaaa
     6901 acgtatttga taaattacat caggaatgag gaaagggcac tttacaaaga aagtaaagtg
     6961 tcctatcatt ttatgtacaa caaaaagagc gtttacgttt tgttggtaaa taataaatcg
     7021 cttacgtctg gtgtctaagg atatatgtga tttcttgaag aaggagtgaa gaaatgttag
     7081 aagtcaagga tcttgttaaa acttttggga actacacagc tgtcgatcat gtctctttcc
     7141 aaatccccga tggaaaaatc atggggctaa ttggccaaaa cggggctggg aagacaacga
     7201 cttttcgttt gatcttggat tttttgacac aagaccaagg agaagtgtta tggaatggcc
     7261 atcatcttgg ggagaaagat tatgatgtca tcggttatct tccggaggaa cggggacttt
     7321 atccgaaagt aacgattcaa gaccaactgc tttattttgc agaacttaga gggaaatcaa
     7381 gaaaagagat tgaaccaaaa atcgattttt ggatggaaaa atttcaggta aaagggaaaa
     7441 agacggataa agtaaaatct ttgtctaaag gaaatcagca aaaagtccag ctgatcgcca
     7501 cgttaatcca tgaacctaaa ttgatcattt tggatgagcc attcagcggg ttagatccgg
     7561 taaatgctga attgctaaaa gatggaatat tggaattgaa aaaacatggt tcatgtgtca
     7621 ttttttctag ccacaatatg gataatgtag aaaaaatctg cgatcatttg atcatgttgc
     7681 gaaacggaaa aatggttttg aatggaaaag tacatgagat tcgtgaatca tttggccgta
     7741 cgaagttatt cttggaatct ggtttaagcc aacaggaagt agaagaaatc gaaggcgtga
     7801 aaaaagtgat ccttcatgaa gacaatacgt tagagattac cttagaagat cctgaagctg
     7861 ggaaagaaat ctttactcgt gcgactcagt tcggctacat tcctatgttt aaccagcaac
     7921 cgccaacttt ggaagagatt ttcaaattga aagcaggtga aatcgatgcg taagttttgg
     7981 attattacca aagatgtcta tttaaaaaat gttaagtcga tttctttctt gatcatgata
     8041 ttagtccctt ttatcatgct gggtatcatt tatgtagcag gaaactttgc tcaacaaaat
     8101 agtgatgtca ataaaatcgg cgtattggct gataatgagc aaatcagtca gcaaatgagc
     8161 caagtcaaaa cagatgattt tgagttcaaa gcagttgatt ctgaagaaaa agcacaaaaa
     8221 gagctttcgg aagaaaaaat cgatgcgtat ttagtagtct caactgagaa tgataagata
     8281 aatgggatgc tttatagcga aaattcatta ggacaaagta cacaactact gatacaacaa
     8341 caactgactg gcttgcaatc gatgcttcga gcaagtcaat taggcatttc accagaagaa
     8401 gtagctagct tgagccaaca agcaagtttg actcgacaaa aagtcaattt tgattcaaaa
     8461 ggaaaaatga cagttggaga agataactcg acagtccaat atgtcgtaag ttatgtagca
     8521 acgattgtct tgttcattat tattttgacg tatgcgcaga tcattgcaca agagatcgct
     8581 tctgaaaaag gcacgcggat catggaagtg atcctgtcca gtacgactgc acaaaagcat
     8641 ttttacggaa aactaacagg tgttttactt gttgcattaa cgcaaatggc attatacggt
     8701 attatttttg gtataggata cagtcaattc aaagatatgg aaatggtcaa aaatatatta
     8761 gacggtatct cattggataa cttgattgga ccattcttgt ggtattctct tctattcatg
     8821 atctttggca ttttgatttt ttctgtttta gcagctttat gtggctcttt ggtcaacaaa
     8881 gcagaagata cagcaaaagc aattttgcca gtgacttatc tatctctagg cggatatatg
     8941 cttggattga tactaggagc ttcagatcca aacaatatcg ttattcggat cacttcgtat
     9001 attccattct tatcttcata tattatgccg gttcgcttag caaatgagac agtcggtatg
     9061 ggtggagcat tgatttcctt gttggtactt gtagccgtaa cattcttatt gatgatcttg
     9121 tctgcaaaca tgtacaaatc gaatgtactc gtgtatagcg aaggcggatt atggagttca
     9181 ttgaaacaat cgatttcaat tatgagaaat gaacgaaaaa aagcataaaa tatagaaaaa
     9241 tatagaagga agctggacat ttattgttcc cagcttcttt tttgtataga cctatttcta
     9301 tttttttatt atattggata tatatattca ttgaaaatag agcataacaa ctttataatg
     9361 ttgagtatac caatttttgg aggcgtttta atggaccgct atttagattt actttcagag
     9421 aagttcccta caacggaaga tgtcatcacg gaaatcatca atctcgaagc aattttgcag
     9481 cttccaaaag gaacagagtt atttttgagc gatatacatg gcgaatttcc tgcttttgat
     9541 catattctgc gtatcggttc aggaaatctt aaagaaaaga tcaaagacct tttctctgaa
     9601 caaatgacgg aaacggagat cagccaattc actattttta cggcttatcc agaatatgtg
     9661 ttgacgactg attggtacaa aaaacaagat aaatcccagc tgatccatcg tttgatcgat
     9721 cttttacggt ttacgacagt caaatacact cgatcaaaag ttcgaaaagg attgccgaaa
     9781 gaatatagct atatcattga ggaactcttg tatatcgatg atcgtatcaa cgggaaaaaa
     9841 gactatgtaa aaaaaatcat tgaacaactt gtcttgatga aggaagaaga aaaattttta
     9901 acaaaacttg cgcagacgat ccaaaaatct gtcatcgatc atttgcatat cgtgggtgat
     9961 atttttgatc gaggaactca agcagccaaa gtaatggatc gaatcatgga tttttcctct
    10021 gtagatatcc agtgggggaa tcatgatatt ttgtggattg gtgcgtattg cggctctgag
    10081 gcatgtttat tgacgctttt acggattgcc gcacgctaca actatttatt tgaattagag
    10141 aaagaatatg gattaaattt acgtccatta ttttcttttg cccatcaaac ctatcagaag
    10201 aatccagtat ttacccctaa aaacaggaaa gatctatctg aaagagagca agaagaatta
    10261 gaaaagatcc atcaagcact ttctatcctc caattcaaat cagaacatca gttgttgaat
    10321 cgacgacctg aatttaaaat ggacgatcgt aaattgttgg aaaaaatcaa ctatgaggaa
    10381 tcaacgatcg ttttagaagg tcagctctat gagttgaaag ggacttgttt tcaaacgatc
    10441 cagccagatg atcctaaaaa attcttggaa gaggaacaaa aagtgatcga tagtttgatg
    10501 tattcattcc agcactcgtt aagaatgcaa aaacacatga cgtttttgat cgaaaaaggg
    10561 ggaatgtatc tcacgaacaa tcagcatgta ctgttccatg gctgtatacc tgtagatgaa
    10621 aaggggcagc tcttagcatt tgaattagac caatcttatc gcggaaaaga actgctggga
    10681 ttttttgaaa aacagataac tgaaagcgca aaagatttga ctgcaaatga agatttggcg
    10741 accgatttga tttggtatgc gtggagcggg ccattttcgc ctttattcgg caagagtaaa
    10801 atggcaactt ttgagcgcta ttttctcaca gagcctcata cgcacgtcga aaaaagcaat
    10861 ccctattatc acttgcgaga tgaagagtgg ttcagtgaaa aaattttggc agaattcgat
    10921 gcaaaagaag aaggttcagc aatcatcaat ggacacacgc cagtaaaagt aaaaaaagga
    10981 gagtcaccga tcaaagcaaa cggaaaagca tttgttattg acggaggtct atccaaagct
    11041 tatcaaaaaa caaccgggat tgctggctac tcattactat gcaattctta cggtttccaa
    11101 atcgtgacac attatccgtt cttgcctatt gaacaacaat ttgcaaaaaa atctgatcaa
    11161 acgaatgtaa aaaaagtgat cgagcagcag ctcccacgca aacttaatcg gaatacgaca
    11221 aaaggaatgg aattacagca acagattact caactccaaa ggctgttgga ttatcgaaag
    11281 gacgattgag aaaaatgcta gccaacattg ctcaatagac taaaaagcga ctgcaacaaa
    11341 aggcgtgtcg cagtctcctt cggaattagc tcaaaaaacg gtaaaatatt agaagctatt
    11401 tttctgttta tttctctaat actcaggtca gggcgctgat ttcacgacct cttccttaaa
    11461 cggtgttctc ctgccaactc ctcggtcaca agtcacaata tccaacaaac atgagaagct
    11521 gttttttgaa attgctcctt gtgactcaga gttgaacggc aggctcacga cctaatcaga
    11581 aaaacgatta aaattcattc acaaagaaat cgaataaacg ctgttcagat gaatctacac
    11641 gatgagaaag ttccggatgc cattggacac caaggatttt gtgcatatta tctttacttt
    11701 ggactgcttc aatgagtcca tcgttggaaa aggcgatggc ttttaacaaa ggagaaagat
    11761 ctttgactgc ctgatgatga taggaattca caaagtatga atcagggaga aggtcactta
    11821 ataaactgtc cgaaacgatt ttgacttcgt gagttgcaaa ctgcggggca gtgggctgtt
    11881 gttcgtgttt tacggaccat tttggatata atgaaagatc ttgataaagt gttccctcca
    11941 acacgacatt caatagttga agtcctcggc agactgcaaa aatcggtttt ctttgtttca
    12001 atgcttccaa gatcaatgcc tgttcgaatt ggtctcgttg aatatttgtt tccgtcagtt
    12061 ttggatgagg gtcttccatg taaagttgcg gactaacatc ctgaccgcct gcaagaatga
    12121 gtttgtcgat tttctcaatt agttgaggcg ctgaatctgg agacatgact ggcagaataa
    12181 gaggtaatcc ttgagcatct aaaactgcat ccacaaatcc ctgcggtgta tagcttactt
    12241 gatttcattg gaaagtatct gtcgctcgaa tcagttgatt cccggcaatg ccgatgattg
    12301 gtttcatttt tattccccct aaagtagact aattgtaaca aggatttaaa aaaatttaaa
    12361 ctatgtaaga cttgccccta tacttatctg ttcttgctgt gttactatct gtaaagagaa
    12421 ttttcgtgtg aaattcatga cgatagttga aaatattgct taatttgtta taataaaaag
    12481 taagcagaaa taaaaaggtt gggcgtaagc ttcaaggatc tagttcttca aggggtgaga
    12541 aggacatttt attgaagggg cctgcctata ctaagttatc tttagaggta catactttca
    12601 atgaaaaaag aaagcatgta cctctttttt aaatcgaaag gaagtgaagc aagtgttaga
    12661 agctttggaa caaatccgaa aagcagaaga agaaaatgag aagcgtgctc gaaccctacg
    12721 gcagcagttg aagacctatg cagaagaaaa agagcaagaa ttggagataa tccaaacgaa
    12781 cagtcggcaa cagctgaaac agttggtaga agaaaaagaa caattcgaaa agcaagaatt
    12841 agaaaaagaa aaaatgaaat tgaaagcaga agcagacaag gtgcaagcaa ctttgaaaga
    12901 ccaatatcaa aagcatcaga gtcaagcaat cggtgcaatc attgaaaggg tgaaagaaac
    12961 ttatggccgt cactaagatg gaaaaagtga ccctcatttc agataaaaga aatcaggaga
    13021 ggattttaca agcagtacaa ggaatccaaa atgttgaaat ccgggatctg tatcaaaata
    13081 gaacaaacaa ccagtgggta gaaaaatatt ttccagatac cacgattatt gataaagaag
    13141 caaaattaag cgctttaggc aatcggctga aagacatcca agaagctatc caattcatac
    13201 aacaccacgg cgatagccgt caaaagaaac tccatttgaa acatcaagaa ctttccttac
    13261 aagacttgga agcaaattat tccgaagaaa atttcatgaa taaattagaa gaagtccttg
    13321 atttaaaaga aaagtgggaa cagctaactg aaaaacgtga tcgtttagaa gaagaggaag
    13381 aatggcttct tcattggcaa cagctggata ttgctccaag tatgtatgca agtaagacga
    13441 ccatttttga gatggggact gtgagtgcag caaattttga aagtttcaag gaagaactaa
    13501 gtaagcttga tgacgtttat atggaagaaa tagattatac gccaaaacaa gtttatcttt
    13561 cgtatgttgc tttaaaaaaa tcagttgctg ctatagaaga aattgctggg aaatacgggt
    13621 tcaataaaga aacgtatcct tatgaacaaa atcctaaaga aaaattgcaa gaaaccaaac
    13681 agcaattatc cattgttcac gagcagcaaa aaaagataac gacagcgtta ggtcagtgca
    13741 gcggctatat ccaagaattt gaatgggtag aagaagtcac acttgccgtt gcagaacggg
    13801 aaaaagtaaa agaacggttt atccatgcca cttatctgat cgttttgcaa ggttgggtag
    13861 atgcagaaga aaaacagaat ttgatgcatg tgttaaacga aactgtatca gaagaagata
    13921 tatacgttag tttcgaaaca ccaaactcag aagagattca gcaggaagtc cctactaaat
    13981 tgaaaaacca tccgcttatc gaaccttttg aattgctgac tgaaatgtac agtctgccaa
    14041 aatatgaaga agtagatcca acgccttgga tgacaccatt ttatcttgtc ttcttcggga
    14101 tgatggtcgc agatgttggt tacggattgc tgatgctcat tgggacaata ctagctcaaa
    14161 aactatttgt tttaccccgc gggatgaaac gtttcgtaaa attctttgag attctctcgg
    14221 taccatcgat catctgggga ttgatctaca gttcattttt cggacaggca ttaccaaaag
    14281 aactatttgg tatccgcttg cctttcccaa tcctatctac gacggatgac gtcaatacga
    14341 ttttgatttt atccgtgatt ttcggtctga tacagatctt ggttggatta ttcgtggcag
    14401 caaaggaaaa tatcaggagg aaagattact tgagtgctgt cagtgatggt tttgcctggc
    14461 aagggatcct gattgggatc gttattgcat tgattggtgt catgttgata aaaaatacag
    14521 catttgttta tcttggcgcg gggctatcga tcattgctgc tttatgtatc ttaatcgtgc
    14581 cgattatcca atcgccgtct aaagtcaagg gtgctgccaa aggagcgtat aatctttacg
    14641 gattgactgg ttatatcggt gatttagtca gttatacacg tttgatggca ctaggtattt
    14701 caggcggaag tattggcgcg gcattcaata tgctagttgc tttcatgccg ccagccgctc
    14761 gattcagtgt agggatcctt ttgatcatcg ctttacacgc actgaatatg tttttgacac
    14821 tgttaagtgc ctatgtccat ggtgcacgat tgcagtatgt cgaatttttt ggaaaatttt
    14881 atacaggagg cggacgtgcc tttgacccgc tgaaaacggc ggaaaaatac gtcaacatca
    14941 atcataagaa aaaaaacaat aaataattgg aggattttta tacaatgatg gattacttaa
    15001 ttagtcaaaa tggtggaatg gtatttgcag tattagcgat ggcaacagcg acgatttttt
    15061 cagggattgg ttcagctaaa ggtgttggaa tgacaggtga ggcagcagca gcgttaacaa
    15121 caagtcaacc cgaaaaattc ggacaggctt tgattctgca attgcttcca ggtacgcaag
    15181 ggttgtatgg attcgttatc gcctttttga tttttatcaa cttaggaaat gatatgtcag
    15241 ttgtacaagg attgaacttt ctaggcgctt ctctgccgat tgcttttaca ggtttgtttt
    15301 caggtatcgc ccaagggaaa gttgcagcag caggtatcca aattttagcg aaaaaacctg
    15361 aacatgctac aaaaggaatc atttttgctg cgatggttga aacatatgcg attttaggtt
    15421 tcgttatttc cttcctatta gtattgaatg cctaagcata aaggaggaaa agcagatgaa
    15481 cgccattgaa aaaatcatct ctcagatgaa tgaagcagca gagcaagaaa gagcggcgtt
    15541 agagcaagaa gagcgaatga agatcgatca aaactttgaa caaaaacgta cgcaaatcga
    15601 aacagaacat caaaaacaaa aagaaaaaca aatcgaatta cttgaaaaaa aataccgtca
    15661 gcttaggaat cgccaacaag tagaagtacg acaagaaaat ttgaatgcga agcaggaatt
    15721 tttacgacga ttgtttgcag atgcaatagc tgaaatggag aattgggatg aatctgaaca
    15781 aatccagttt atcaaaaatt cattgtattc attacctttg acaggcaaag taacattcat
    15841 tgctggagaa aaatcagcag cttatctttc tcagacattg ctggatgaat ggaacaatga
    15901 acttcccttt atgatggttt taagtgatga aactgttgct gatcaagcgg gttttctgat
    15961 aaacgatcaa ggagttcagt ataattttct tttcagcagc cttgtacaag atatccaagg
    16021 aacgatgagt tttgagattg ccaatcaatt attcgaataa ggagggatca gtgtatggaa
    16081 taccacgaac taaatccctt gattcgcgga agagagcttg aacttttgtc caatgagaca
    16141 tttgaacgga tgattcaaac aaattctgtc gaagcacttg gtgaaatatt gaaatccacg
    16201 atttacagtc catatattta tgacggatat gaatatgact tcgaacagaa tcttttaaag
    16261 gagcagagcc gtctatttcg ctggttaaag gaatcagcac cggagcctga aatcgtttgg
    16321 atctatacga tgcgctatac tttccataat ctgaaggttt tgacaaaagc cgagatgaca
    16381 aatcaaaatc tggaccattt gtatatcgac gatggatttt attcattaga aaccttgaag
    16441 gatgccattc atacgcaagt ttcaaatgaa ctccctgaaa gcattatgga cagtatacga
    16501 gaagtacgtg attattttga agagtctacc attcttcagg gaattgacgt gatttatgat
    16561 cgacagtttt tgacagagca gcgacggctt gccgataagc taggatatca agaattgctg
    16621 acagaagtca ttgcttttat tgacttgacc aatatcacaa cgatggctcg aggaatattg
    16681 cagaaaagaa catatgggtt catgacgacg gtactttcca gctcgggaag tatcgaaaaa
    16741 gaagtatttt tgaactttgt agataaagat atggaagatt acacgaattt tctattagag
    16801 acagattatg ctgattttct gcgccctgtc atccgtgagg gaaatcttga tctcgtaaag
    16861 cttgaacagt tgaaagatga ttatctaagt tcattgtatc aagaagcaca aacccaggct
    16921 ttcggaccac tgccactact agcctttttg aacgcaaaag aggtagaaag caaaaacctt
    16981 cgtttactgg ttatcggcaa aagaagccat ttcgaaccag aaacaatcag aaagcgggtg
    17041 agacaagtct atgacgcata agatcggtgt agttggcgac aaagattctg ttttgccatt
    17101 caaattgttt ggctttgatg ttcgttatgg tacaaccaag caaaccgttc gtcaggcaat
    17161 tgaagaaatg gcaaagaatg agtatggcgt gatttatgtc acagagcaat gtgcagcatt
    17221 gagcccagaa acgatcgaac gttacaagga acaaatgata cctgctatcg tattgattcc
    17281 caatcatcaa ggatcattag ggatagggct ggaagaaatt caaaaaaacg tagaaaaagc
    17341 agtaggtcaa aatatcttat agtatgaaaa ggagatagaa attttgcaaa ttggaaaaat
    17401 cataaaagtc tccggacctc ttgtaatggc tgagaacatg tctgatgcca gtatccaaga
    17461 catgtgtcta gtgggagatc taggagttat cggagaaatt atcgagatgc gtggcgatgt
    17521 ggcgtctatt caagtatatg aagagacttc tggaattggt ccaggcgaac cggttcgctc
    17581 tactggtgaa gctttatctg ttgaattagg accagggatc atttcacaaa tgtttgacgg
    17641 aatccaacgg ccattagata cctttatgga aatcacacaa agtaatttct tagggcgcgg
    17701 agttcaattg cccgcattgg accatgagaa aaaatggtgg ttcgaaccaa cagtagaagc
    17761 cggagaaacg gtgagtgctg gagatgttat cgggatcgta gaagaaacaa aagttataaa
    17821 acataaaatc atggtgccaa atgggataaa aggaacgatc aaaacaatcg aaaaaggttc
    17881 attcactatt gatgaaacag tatgtgtcat tgaaaccgaa aatggtgaca aaaatttgac
    17941 gatgcttcaa aaatggccag taagacgtag tcggccaatc aaagaaaaat taaacccgga
    18001 tgcacccatg atcactggac agcgagtgat tgatacattc ttcccagtga caaaaggagg
    18061 agcagcagct gtacctggtc cttttggtgc agggaaaaca gtcgttcagc atcaaattgc
    18121 aaaatgggcc gatgtggaca tggttgtcta tgtgggatgt ggcgagcgtg gaaatgagat
    18181 gacggatgtt ttaaatgaat tccctgaatt gattgaccct aatacaggag aatcgttgat
    18241 ggaacgtacg gtattgattg ctaatacatc aaacatgcca gtggcagctc gagaagcttc
    18301 tatctatacg ggaatcacaa ttgcggaata tttccgtgac atgggttatg acgttgctat
    18361 catggcagac tccacttcac gatgggcaga agcacttcgt gagatgagcg gacgtttgga
    18421 agaaatgcct ggtgatgaag gttatccagc ttatcttggt tcacggcttg cggaatacta
    18481 tgaacgatct ggaagagtca ttgcattagg atcagaaggt cgcgaaggaa gtataaccgc
    18541 aatcagtgct gtatccccgt ctggagggga tatttcggaa ccagtcacac aaaatacatt
    18601 gcgggtcgta aaagtattct ggggtcttga ttccagttta gcccaaaaaa gacattttcc
    18661 ttcgatcgat tggatacaaa gctattctct ttattcaaca gaagtaggga agtatatgga
    18721 tcatatcctt caagctgact gggcatccat ggtcaccgag ggcatgcgta ttttgcagga
    18781 agaacagcag ctgaatgaaa tcgtgcgttt ggtagggatt gattcccttt cagataatga
    18841 ccgtctgaca ttagaagtgg caaaatccat tcgtgaagat tatttgcagc aaaatgcttt
    18901 tgatgatgtc gatacattca cttcaagaga gaaacaattc aaaatgttga agatgatctt
    18961 gacctttggt gaagaaggac gccgtgcgtt gtcgttaggt gcttatttaa gtgagatcat
    19021 ggacggtacc gtatcgattc gtgaaaggat cggacgaagc aaatatatcc cggaaacaga
    19081 attagaaaaa ctagatcaaa ttattggtga aatcaagcaa acgatgcaaa cgatcgtttc
    19141 agaaggaggc atgagcaatg attaaagaat atcgtacgat cggtgaagtc gtcggtccat
    19201 tgatggcggt tgaaaaagtt gctggtgtca aatatgaaga attaatcgaa gtccgtatgc
    19261 aaaatggcga gattcgtcgt ggacaagtat tggaagtcca agaagataaa gcaatggtcc
    19321 aaatattcga aggaacaagt ggtatcaatt tacgtgactc ttctgtacga tttttaggcc
    19381 atcctttaga actaggtgta tcggaagaca tgatcggtcg agtattcgat ggtttgggac
    19441 gaccgaaaga taacggacca gagatcctgc ctgaaaagca tttggatatt aacggagaag
    19501 taatcaatcc agtagctcgg gattatccag atgagtttat ccaaacaggt atctctgcaa
    19561 tcgatcattt gaatacatta gttcgtgggc aaaaattacc agtattttct ggctctggtc
    19621 ttcctcataa agaattagct gctcagatcg cgcgacaagc aacggtactc gattcatcag
    19681 atgatttcgc cgtagtcttt gctgctattg gaatcacttt tgaagaagct gaatttttca
    19741 tggaagattt tcgtcaaacg ggcgcaatcg accgttctgt catgtttatg aatttagcaa
    19801 acgaccctgc aatcgaacgt atcgctactc ctcgtatggc tttgactgct gcagaatatc
    19861 ttgcttatga aaagggaatg catgttttgg ttatcatgac agacatgacc aactatgcag
    19921 aggcattgcg agagatttct gcagcacggc gagaagttcc aggaagaaga ggatatcctg
    19981 ggtatcttta cacgaactta gctactttat tcgaacgagc aggaagaatc cgcggactaa
    20041 aaggatcggt tactcaaatt ccgattctaa caatgccaga agacgataaa actcatccaa
    20101 tccctgactt gactggttat attacagaag gacagatcat cttgactaga gagctttaca
    20161 agagtgggat tcagccacca attgatgtac tcccgtctct ttcgcggtta aaagataagg
    20221 gaactggacc cggaaagaca agagaagacc atgcagctac gatgaatcag ctttttgcag
    20281 cctatgcaca aggaaaacag gcaaaagaat tagccgtagt tttaggtgaa tctgctttat
    20341 ctgatgtaga caagatctat gcgaagtttg ctgagcggtt tgaaaaagaa tatgtcaatc
    20401 aaggattcta cacgaaccgt tcgattactg aaacacttga tctagggtgg gaactgctat
    20461 cgatgttgcc aagaaccgaa ttaaaacgaa tcaaagacga catgctggac aaatatctgc
    20521 cagaagggaa gtgagttctg atggcacgat tgaatgtgaa tccgacccgc atggaattga
    20581 ctcgattgaa aaaacagttg acaactgcca caagagggca taaattatta aaagataagc
    20641 aagatgaact gatgcgtcaa ttcatcctgt tgattcgaaa gaacaaccag ctgcgtcaag
    20701 aaatggaaga gtctatgaca gaagcaatgt ctgattttgt attggcaaat gcttcgatca
    20761 cggaaccttt catcgaagaa ttatttgttt tgccggcgga aaacgttgat ttgtctatcg
    20821 tagacaaaaa tatcatgagt gtaaaagtac ctgtgatgaa ttttgaatat gatgaacagc
    20881 tgacagacac acctttggaa tacgggtatt tgaattccag tgccgaactg gatcggtcaa
    20941 tcgatcggtt tactaccctg cttccaaaac ttttggaact gacagaaatc gaaaaaactt
    21001 gtcaactgat ggcgagtgaa atcgaaaaga cacgtcggcg agtcaatgca ttagaatata
    21061 tgacgatccc tcaattagaa gaaacgatct attatattcg tatgaagctg gaagaaaacg
    21121 agcgcgcaga agtaacaagg ttgatcaaag taaaaaatat gggtaccact gactaagtgg
    21181 tatctttttt taaaagagga aaacgatgat taaacaaagc aaacaaataa agaaacgttt
    21241 gccggctgtt cagcttctcg cattaggatt ttttctcctg attttagttg gcggcggcct
    21301 acttacccta ccatttttta gtaatagtgg gcatgggaca aattttatcg atgcgctttt
    21361 cactgcaact tctgctgtct gtgtaacggg attgacaaca ttgaatacag cagagcattg
    21421 gaacagtgct ggtcaatttt tgatcatgct tttgattgag atcggcggtc ttggttttat
    21481 gatggttccg attctatgct ttgcattgat aaagaagaaa gtcagtctca gcacacgtat
    21541 tatattaaaa gaagcgctga atctagaaga agtatcaggt gtgatgaagc tcatgcttta
    21601 catcctaaaa ttggcctttg ttatccaatt gattggtacg gttgctttat cttttgtatt
    21661 tgtgcctgaa tttggctggt tgaaaggtat ctggtacagt ttgttccatg caatctctag
    21721 tttttgtaat gcagggtttg atttgttagg agacagttta gccaatcacc agcaaaatgt
    21781 ttatttgatc atggtggtat ctgctttgat catttctggt gggttaggct tcatcgtatg
    21841 gcgtgattta ctggaatatc atcagaaaaa gaagatgacg attcattcaa aaatcgcatt
    21901 aaccgttaca ggtacccttt tattaggcgg attcgttgtg ttctttttca cagagcgcaa
    21961 tggggcaacg ctagtggaag ggaatatgtt tcaacgattg gcaaacacct tttttatgag
    22021 tgtgacacct agaacggctg gatattattc agttgattac ctccatatga gccatgcagg
    22081 tctgatttta acgatgtttt tgatgtacat cggcggaaca tctgggtcga cggccggcgg
    22141 attgaaaaca actacgttag gtgtgttgct gatccagatg cgttcgatgt tgaaaggacg
    22201 tacgagagcg gaagcatttg gccgtaccat ccgacaaggc gcagtgttgc gggcacttac
    22261 tttatttttt gttacgttga cgttgtgtgt agtcgccatc atgattttat cggtgactga
    22321 aacgatacct aaaacttctg gcatcgaata tattgctttt gaggtcttct ctgcgtttgg
    22381 tacagtaggt cttacgatgg gattgacgcc agacttgact gcaataggga aattgatcat
    22441 tatagcgctt atgtacattg gacgagttgg tgtattgact gtggtcttct ctctgatgac
    22501 aaaagcacaa agaacacaat caaaatacaa atatccagaa gaaagtgtca tgattggata
    22561 atttcatctt ctgacagaaa cctagtgatt tagtaaaata agaaaataaa ataatttttc
    22621 ttgcttcttg gatactttaa gtgcaaaatg ttcgtttatt ttttgaggca ctacccgttg
    22681 ttgaacctcg ggtagtgcct cttttttatt gaaaggaatc aataagattt gattccttta
    22741 tttatcaata ggttataatt catatttttt tattttttct tttacgatac ttagtccaga
    22801 agctaaaatt tcaaggttat tagaggatag ggaaattcga acgaatgcat ctggttgcgc
    22861 acgtgtagta aagcgtctgg aatgataaag tcggataccg ttagcaagaa agtcattctc
    22921 aattatctta gggtcaatct caggatcaac tggaagaatt cgatagtaag ggacacgatt
    22981 cgttggtttt tctactttga aaagagaatc aaaaagctga ttggctcttt ttgtaagact
    23041 taatttagtt tctaagatcc tatttgcttc atttgaatgg aggatttgag tgatgacttc
    23101 cacatctaat ccagaagttt tcacatttat attaaaaatt gcttgttcta tcttgctttg
    23161 gacatgtact ggaactacaa gataagcaac tcgcaatcca gaacaaataa attttgtcat
    23221 tccagctaag tagattgttt ggttaggtaa taaattttgg aaaggaggaa tgatcgtatg
    23281 ttgatagtaa gttgttaaaa atgaatggat atcatcttca ataacccata gaccttcttt
    23341 ttcaatcact tcggctaatt gctctcgtct tttgtgagag atttgaaacc ctataggatt
    23401 attacaagaa ggcataagaa agacaccatg aatcttcttt tttctacatt cctgaatcaa
    23461 tatatcaggt cgcattcctt gctcgtcaaa atcaattgga acgatctcta aatgatataa
    23521 ctgagccaat tcaatgaaat tagcgtatgt atatcggtct gttgctattc tatcacctgg
    23581 agagaaaagt gcagaaagtg tgatagccaa tccattttga acaccggaaa caatagatag
    23641 agattctaag gacggaatga ctccttgagt ttttaaccaa tttgctgcag tagtcaattg
    23701 aaaatttgtg cctttgggat cttggtaatt aagcaaacca acaagatttg ggttattact
    23761 agttgattga ataaacggga caacgaattt attacattct tcaaaagaac taaccagacc
    23821 aaaatcaatg atatgttcgg ttacttcatt ggtagagatg gtgatggact caaagacatt
    23881 gggagaatta aatgtgccac ttccaatatt cgtatacagc aaacctttct caattccata
    23941 tttataagcc ttgccaacgg tagtaaaatt taaatctaaa taatcagcta gctctctttg
    24001 ggagggtaac tgagtgtttt tctgtaattt attctttaaa atatcttgtt ctaattgttg
    24061 aatcaacgat cgataataag gacgcttcaa tttttgtttg tctggtcgcc aagttaacgg
    24121 gtaattgtca taagaattaa taggcatttt ctccactcct taaaattgta taccatacaa
    24181 ttttaccatt gattgtattg attttagaag ttaatctttt gtgaggaggg aattgtctat
    24241 gaaaattatt aattttgagg aagtagtagc ttcttttagt tttgaagaag cgattcaagt
    24301 aatgaaaaaa tgtttttttg attatgaaag tggtaaaatc agccaaaatc ctcgattggt
    24361 ggaaatttta cctgacggaa aaggcaaaaa tatgtttgcg atgatgcctg cttatttggg
    24421 ggagaacaga tattttggca caaaagtcat taccgctttt ccggataatc atcagtatca
    24481 tttaccttct catttaggag agattttctt gtttgatgct caaaacggac tacccaaagc
    24541 attgataaac gcaaatgcag tcacctggat tcgaacagct gctgtatctg cgctggcaac
    24601 agattactta gcaaatccgc atgcagaaac acttgcttta attggagctg gtcaacaagc
    24661 attgtcgcat ttgactgcga tttctaccat tcgtcccatc actacagtgt ttgtttacga
    24721 tattttagaa gaaagacgaa atacttttat cgctaaagct aaaaaacagt atcctaaaat
    24781 aacgtttata aatgcaatat cagtagaaga tgcagtacgt ccagcggaaa tcatttgtac
    24841 gttgacctct agtaaagagg catttcttca aaaagagtgg gtaagggaag acgcacatat
    24901 caatgctgtg ggcacattta ctcctgatac aagagagata agtacatcgt tgatggaaac
    24961 ttctcaaatc tatgtagatg attactcagc tgccttaaca gaaagcgggg acctgcttat
    25021 agccatggca gaaggagatt tagaggaatc ttgtattgtt ggtacattag gcgaattagt
    25081 ttctgagaaa aaaacatttt ataaaggaga atcgaggccg acgattttcg acgcagttgg
    25141 tctagctatt gaagatcttt gttgtgcaga atatatttat aacaaattag agaaaggggc
    25201 tttaaaatga acttagatta ttacgtagtt gatgcatttg cagatgaggt gttcaaagga
    25261 aatccagcag cggtttatgt gctagaagaa tggctgccag aaggtacgat gcaaaaaatt
    25321 gccattgaaa acaatttatc tgaaacagca tttacagtga agaaaaacca agaatttgag
    25381 cttagatggt ttacgccaga tcgagaaatc gatctttgtg gtcacgcgac attagcaaca
    25441 gcatttgttt tatttaacta ttacaaaata cctgatgaaa cgattaaatt ttctactcaa
    25501 agcggtaatc tatttgtgac aagaattaaa gattattatt acatggattt tcctagtatt
    25561 atgccgaaaa aagtgccgat attgtctgaa tatgaagaag cgattggggc taagattaaa
    25621 gaagcctact tagcacgtga tttatttttc gtattagaag atgaaaaaac ggtggctaaa
    25681 ttgcagccag attttacagc tatcaaaaat tttgaattag gaataggagt catcgtgaca
    25741 gcagaaagtg tgcaaaaaga ctttgtttct agaaccttct ttccaaaatt aacaatcaat
    25801 gaagatccag tatgtggatc agcacattct aatcttattc cttattgggc agaaagaatg
    25861 aataaaacaa aattgtcagc ttatcaaatg tcatcaaggg gcggggatct ataatgtgaa
    25921 ttaaaagaag atcgtgtgat aataggagga aaagcagttt tgttttctaa aggtacctcg
    25981 tttatttgat ttctttcaaa tgaattaaaa ataaattggt ggagagtggc aaccttatag
    26041 ctacaatggt tgttaaaaaa agacgcaaac tttttcaatt taatgaaaag tttgcgtctc
    26101 attttaatta ttggtgtttg ttttttattc aatcaagagt aaaatgcccc atactaacta
    26161 tcctcggatg acttcgattt tataaccatc agggtcaatg acaaagtagt aagaaggcgc
    26221 agtgccaggt aatcctttta agtcagtaat ctcaagaccg gcagctttat gttgctcgtg
    26281 taatttttct agatcatcag ctgctatagc gatatgacca tagccgtctc ctagatcata
    26341 cgcaccatga tcatagttgt aagtcaactc taattcatag tcatccccag gaagtgtcaa
    26401 ataaacaagt gtaaatttgt tttctggaaa atcacggcgg cggctttctt caaaaccaaa
    26461 tgccttttga taaaattcga ttgatgcttc aagatctttc actcgaacac atgtgtgagc
    26521 cattttcata gattcatctc tccttttcct tttactcaat cataacaaaa agaagtaagg
    26581 aaatagttga tttgtgcttg ttcatgagaa atttctcatg taaaatggat agaaaataaa
    26641 actgttaagg tggaaactat tcatggataa accaattttt ggtgaaaaaa agcaatttgc
    26701 tgattatcaa acccgttatg cagcatatat tgtcatagaa agagatcagc agattgcagt
    26761 aatcgaggct ccaaatggtg ctttttttct tcctggcggg gaaattgaag gagaagagac
    26821 gaaagaagaa gcgattgtcc gtgagctgat agaagaaatg ggaattgtgg cagaaatttc
    26881 ttcttatctg ggagaagctg atgagtattt ttactcgaat tatcgtgcaa cttattatta
    26941 taatccagga tatttctttg ttgctgattc ttggaagcgt ataggagaac caactgaaaa
    27001 gaccaataaa atttggtggg tgagccctca agaagcgctt gtgaaattga aacgtggtag
    27061 ccaccgatgg gcagtcgaga gatggatcga aaaaaataaa aactgaaaga aagcgagtag
    27121 atcagctttc tctcagtttt tttatttatt acagtacata ttttttgatt gcttcagcta
    27181 caccgtgctc gtcgtttgat gaagtgatga catttgctgc ttctttgaca agagaaacgg
    27241 catttcccat tgctactcct agaccagcat attcaatcat ggataagtcg ttctcgttat
    27301 ctccgatagc catgatttca tcttgatcaa taccaaggtg ctgtgcgaga ttcgcaactg
    27361 ccgcaccttt acttgcttct ttatttagta cttcgaagta atagggcgca cttttgaccg
    27421 ttgtgtattt atcacggaaa gtttgcggta aacgcgcaat tgctgcatct aaaatttctg
    27481 gttcgtcgat catcatcatt ttgacgatgt tcatgtctgg tgtcatttct tcaggtgttc
    27541 gatattttag cggcatcttg actaaacttg cttcgtggat cgtataagcg ctgatatctc
    27601 gattaggcgt gtagatcgct ttgtcatcga tcgaatggag atgactgccg acacggcgtg
    27661 ccatcacttc gatctctagg aaatcttcat gtgtcagtcc gtgacgggcg atgattttac
    27721 ctgatttcgt ttgttgtacg agtgccccgt tataagtaat aacgtaatca ttatcttgaa
    27781 aaaggttcaa ctcgtttaac tgttcctgga cacctggtaa gggacggcca gtgcataaaa
    27841 cgatcgaaac accttttgca acagcagcgg caatcgcttc ttttacttct tttgttactt
    27901 ctcttttctc atttaataaa gtaccatcga tgtcgatagc gactagtttg atagacataa
    27961 tttcctccaa tatattttaa tttatttcaa tcaatgcgcc attacggata tggctggcaa
    28021 attcttgata agtttcgtca aataaatcat aatgatcacg caaggacgga tcaaccattt
    28081 cttttggaaa gaaaaatcgc tcatctcctt gttcttgtcc cgctaatgca gcaacaagct
    28141 gactaacctg tgataattct atcaaagttc cgtcatttcg caaaatctcg atttgtgtgc
    28201 gatgtctgcc ttgttctggg cgataaaaat cataaggtag atcgtagcta gaattcacag
    28261 cagtgtaata aacaggatta tatccgacct tttctactaa caaagtgagt tcttggacta
    28321 attttgaatc ccggttgcca gaaaaagtag cagatttcag tggtttacga ttcaaaaaac
    28381 gtttagctaa atcatttaaa ataggatcag cagattcaga ccattgagta aagtaagtgc
    28441 ccaagacccc atcatcaagt ttgaggtatt cttctaaagt aaagcttccg tcaagaaatg
    28501 gcagaagtaa ttgagaatgg agttcaaaac tgctggcatc ttcttgatac aactcgtgtg
    28561 cgcgatgaag cagatgctcc aaaattactt ccataccacg ggaaacagga tggaagtaca
    28621 cttgcacata catttgatat cggctaacga tataatcttc cactgcatgc atgccactca
    28681 tggaaaaagc gatcccgcct tcataaggac gaatcacacg taatattcgg gtaagatcaa
    28741 atgttccata ttctgttcca gtgaaataag catcccgaag caaatagtcc attcgatctg
    28801 catcgatctg actagaaatc atttggacaa cttgtgggtt ggggtaagtt ttttggatca
    28861 cactagctac cttttcagga aaaccttctt ctactcggtt aagaatctga tacacttctg
    28921 tttctggaga agtgatgatc tctacagtga tggcttcatg attcgtatgg aagatatgtt
    28981 caaatgtatg agagtaagga ccatgaccga catcatgcaa cagtgccgca cataaagtca
    29041 ccagccgttc ctgatcatcc cagcctccgt tgcctatttt ttcttttgaa aaatttcgag
    29101 aaaagatatc gcagattcgt cttgaaatct cataaacgcc taatgaatga gtaaaacggc
    29161 tatgttctgc tccgtgaaaa gtgaaagaag aagtgcctaa ttgtttaatg cgtcgtagac
    29221 gttggacttc tttcgaattg atcaaatcta agattacttg atgctggaca tggatatagt
    29281 tatggactgg atcacgaaaa actttttcca taggcagcat ttgatattta taaggggttg
    29341 tcataatgta gctcctttat gttattacgt tgttccattc tctggatttg gttgttccat
    29401 tgctccagct ggatttcttt ttttagccag tcggtagaat cctgtaaatc acttgcagaa
    29461 tacagctttt caaaagcttt tgcaaatgct tctttgactt cttttacaaa aaggggttgg
    29521 tcaagcagtt cttcaagtgt tgccattgta gcaggatcca ctgcaggata gccatttgta
    29581 ccaaagtctt cttttaaagc agattgataa aatcgcttca tgacttgccc gcgtttttcc
    29641 tgattaccgt tgacacttaa gtacatcata acagaaatcc catttttgat ccgacgctga
    29701 gcgataccgg caaatttttt gccatggatg cttaaatcat aagttccagg acaataagaa
    29761 tgggtgattt cgaatgcttg tatcggtgcc tttggaaaag cgtatttagt caataggagc
    29821 atttgctcgt aagcgtcatc tatggttatt ttttttgtgt cactttgggg gaaaatcaaa
    29881 gaaatattca aaattccttc atctgatacg atccctaatc cacctgaatt tctgatcact
    29941 ggagcgtaac ctgcatcttt cagtgtttgg atcccgtcac ttagaaaagg agttcgaaca
    30001 tccttcatac ctaagatgac tgtgcagtca tattgccaga aatgtaaaaa gatttgctgg
    30061 tgttctttag aatattccgt taatatatct gttaatgcaa aaggggaaaa gtcgtttttt
    30121 tgataaatgt gctgatctag tcgaatcgca gggttcattc aaccggctcc tttgtataaa
    30181 ttcatatttt ctacaatatt atactctaaa atcaaagagt tgttttggaa aaaaggtttt
    30241 ctgtaaaaat ggacaagttg gttgacaaaa caaaagaaac taggaacaat gtagctatga
    30301 gaagtaaagg agaatgaata atggaattat cacaaggaac accgatttca atcaaattgc
    30361 gtacaaaagt aaaacagcaa ggggaagtgc aagattttta ttttgatttg aaagggcaaa
    30421 tggtgacaat tggcgatacg ctgtacatac gttataaaga gattcaggag gagacgggcg
    30481 atgagattcc tgtaacgatc aaattagttc cagatggtca tgtacagctg atccgtgcag
    30541 gagaaatgcg gatgcgtttg aaatttggct ataaagaacg gttagaaaca tcctatcgaa
    30601 caccatacgg tatgatccag attgctactt ttacgaaaga actgcatgtt agtttgaaag
    30661 accgtcccac aggagggaag gttcgaatcg attatgatct gttcatggga ccggaaaaaa
    30721 tcggcgaata ttatttgacc ttggatttta cagcgtaaat ataaaagaaa agtttgattt
    30781 tttgtgtctg attttgtatg ataaggagta gactgtgaaa ggacgtgtcc tgattggaaa
    30841 ttagtgtatt tgaaggtgca aacaaaagcg aattatcaat gatcgaagta gcacacgcga
    30901 tcttagaaca acgtggcgat gtcatggatt tttctgattt agctaaccag atccaaaact
    30961 atcttgggaa atcagacagt gaaattcgtg attcactcgc acaattttat actgacttga
    31021 atattgacgg cagctttatc tcattagggg ataatcgttg gggcttgcgt tcatggtatg
    31081 ctatcgattc gatcgatgaa gaagtaaacc atggaatgga cgaagaggat gaagatactc
    31141 cacgtcgccg taaacgcaag aaagtcaacg cctttatcaa tgatgatgaa gacgcgatcg
    31201 attacaatga cgatgaccca gaagatacag atctgacaga agaagatgac gatgatctgt
    31261 ttgatgacga tgatgatgaa gatgaagaaa tcgctgctta taattctgac ttgcaagaaa
    31321 tcggagcaga tgacccgaca gatgatgaag aagacttgcc aggaaatatc gaagaagatt
    31381 taagtatcat agacgaagac gatgatgatg aagattatga agaggaagaa ttttctgact
    31441 ctgaaaaaga ataaacatta ttttcttacg agaggttgtg acagagacga tcagcctatc
    31501 agaataagga ggtgtgacac gatttttgtc acaccttctt ttttgtatat aaaaatccca
    31561 agtaaataca taaaaaataa aaaaattgaa acttttagcg caccttgtac gtcatattaa
    31621 gtgaggcaga aaggagtgga gaatgatttt tactggatat aagaaaaaac ggcagatcga
    31681 aaaataccgt cacctgctga atggactcat cgaaactgat tatcaaaaaa tgttccgtat
    31741 cacgatgaat tacgtacatg ataaagaaga ggccttagat gtgatgcaag atagttttca
    31801 taaagccctg aactattttg aaaaagaaaa agagatcgag cattttagtg catggtttta
    31861 tcggatattg atccgcacgg cattagatag ttggcgcaga caaaaaagga cgcctgtgga
    31921 cttatttgaa attcaagata tcccccaaca gttaccttta gaaacttccg ttgcagagct
    31981 ccatatgatt ttagataaaa tagacagtcc ggaaaaagag attctgatcc tctacttttt
    32041 tgaaggattt cgtttgaaag aaattgcgac tatcttggat atgaatgaaa acacagttaa
    32101 aacgaaaatg taccgctcat tgaatgcttt acgtcaaatc ctagaatgaa ggagagaaga
    32161 gtgccatgta ttcccaaaaa gaaaatttaa aaaaactaaa gaaaacttat aaagaacaag
    32221 caattcccaa agggcttaaa gaggagatcc gccagcgttt tattatcgaa gagcaatcat
    32281 ttataaaagc aaagcggaga aagagacgtc tccaagggtt cggagtatct ttggctatcg
    32341 taatgattac aacaagtagc cttcttttta atagtcaagt tcgaagcttt gcagaagatt
    32401 tacccatctt aggatcagtc attgaattga tcttaggcga acgctttact gatcgatctg
    32461 aaaaaatcga tattcaggta ccgaagatca atacacaaaa tgagaaagaa aataaaacaa
    32521 ttcatggatt aaatcaaaaa tactttcgcg aaggacaagc agattttgaa aaagctgaaa
    32581 aagaatatgg aaattttgaa acggatcatt accaagtttt gggagattat cagaaaatcg
    32641 tagacgataa ccggtttctc gtgattgaaa ggcagatcac ccaaacagca gcagatagcc
    32701 atgttgaaaa aagatacgac acaatcgaca agaaaaacag tgtccagctc tcacttccac
    32761 ttttattcaa agatgacacc tatctatctg ttttgacaaa agaagtcaaa cggcagatgg
    32821 ccgaacaagt caaggaagat ccatctaagt actattggac tgagcaagat attcaagaag
    32881 ggacgataga aaaaccaaca cttgttacgc ctacacgaag tttttatttg aacaaagagc
    32941 atcaattagt tttgactttt tctcaatacg aaattgctcc tggttatatg ggtacaccag
    33001 aatttgtcat tcctaagtcc gtgacaaaaa cgattttggc atcggaagat tatttggatc
    33061 attagacgag aagtagttgc gactagcagt ttcaattttc aagtaagtac aagtaaataa
    33121 taagaagaga aaaagagaaa gaagagatat agtgaaaaga aattatttag ttatcagtat
    33181 tattgcttta tccgtgcttt tgtttttcga aattacttcg gtggcctcct tgatcaccga
    33241 agtaaaagga caaacacact cttcggagca ttcgtcacaa tctttcaaac aatcagaaca
    33301 atcaaccatt aaaagccaat caactgaaac agctaacgtc aaagaagcca ttcaagaatc
    33361 agcggaaaag gtacaaagtc agtcatcaca atcaacacaa gacagtacaa aaacgagccg
    33421 agatccggct gtcaaaagcg tcgcaatcag ctttgatgat ggaccaggag cgaccacaac
    33481 accgcagctt ctgcgaatct taaaagaaaa aaacgtgcat gccacttttt tcgtcttagg
    33541 agaaaatacg gcgcagcatc ctgaaatcgt gaagcagaca gctgaagcag ggcacgagat
    33601 tggcaatcac acatacgatc atcagaatct ggccactctt tctgcccagt cgatgacgga
    33661 ggaagtgacg aaagcagaca ctgagatcaa aaaagtcact ggcaaaaccc ccacttttgt
    33721 tcgtccgccc tatggttctg tcacgaatgt aggagcaacg atcatccagc gaccgatcat
    33781 cgaatggtct gttgattcag aagattggaa gacaagaaat cctgatttga ttttgcagaa
    33841 gatccaagca acagtatacg atggggcaat tattttattc catgatatct atcctgaaac
    33901 gattcgtgcc gttcctcaag tgatcgatta tctgaaagag caagggtatc gcatcacgac
    33961 agttggggat ttattagggc atccaacggc tgtcgagaac tattatggca gaaacgatca
    34021 tcggccagtt cagtaagtga gacaagtatc tttaataaat aacacatttt aataagtagt
    34081 tgaacacata actaccttac ttgcaaaggc ttaacaatat ctatacaggt acaacaaaaa
    34141 actgtccacc aattttcttt ggcggacagt tttcacttaa aaataaagaa gcaactattc
    34201 gtaaattatg ctaaaatagc agtagaacgc gtcctgaggg aatcgaaccc ccgtctcaag
    34261 aaccggaatc ttacgtgata tccactacac taaggacgca agcactggga taattgtaac
    34321 taaacagata aaaaattaca aggggattgg acaaaaaaat gaaatttgag caaaattttt
    34381 cacaaaaaca gcagcagaca cagaaactaa ccatgacgca acagttgcaa cagtccatcc
    34441 aagttttaca atataacagt gaggagctgt tggcattcgt tgagaatcaa gcaatggaaa
    34501 atccattggt ggaagttgtt gagccggaat ggcagccaga ttacataaaa gcttcttcat
    34561 cttcttatga aggagaagag acaaattacc taaatcagat tcctgataca aaagggtctt
    34621 tatttgattc attgatcgaa caagtccatt tgaattaccg agatactttt ttgaggaaaa
    34681 tcgttcttta tcttgtagaa tacatcgatt tgaacggatt tttgacgatt agtttagaag
    34741 aagccggaaa agagattgga gctacgccta tccaaatgct cgatgcgttg actctgattc
    34801 agcaattaga accagctgga gtaggtgcac gtagtttgca agaatgtctc atgcttcaaa
    34861 cagaacgaga tgattatgcg ccggaattag cttatatcgt gttggaagaa tgttttgagg
    34921 aactggtgga gcgaaaatgg aaagaaatcg cacaacgttt ttctgttgat ctacatgctg
    34981 ttcagcagat attcgactat ttacaaacgt tgagtccaag ccctggccgg attttcgacc
    35041 gtagcagcga attgtacatt attcctgatg tccgggtatt agtggacgac gataaaaatg
    35101 tacaagttat ctcaaaccga aaaaatcagc caaatatacg ctttcaagag agttacttca
    35161 aacaaatgtc acaacaagca gataaagaaa ctgaaaatta tttaaaagaa cggaaacagg
    35221 aattcgaatg gttgaagaaa acgatcctac aacgtgggga tacaattttg cgggttgctc
    35281 aagtcattgt ttcccggcaa aaagctttct ttatcgacaa ggaacgtcca atcaagcctc
    35341 ttacgttaaa ggaagttgca actgagatag atgtgcatga atccacggtc agtcgtgcag
    35401 tcaacgggaa atatttggaa acagacttcg gtgtatttga attaaaaaaa tttttcacta
    35461 cacgaattcc tacgaatagt acagaacaga cagaagacct ttcagcagat acagcgaaga
    35521 aaaagctgca ggaattagtc gatcaagaag acaaaaacaa gccgttgtct gaccagaaat
    35581 tggttgaatt gctgaaaaaa gatgagatag cgatctcaag acgaacagta gcaaaatatc
    35641 gtgatttatt aggaatcccg agttcatcaa aaagaaaacg ttatgataac taatcagtca
    35701 aaagactaga aaaagtattt cactttttct agtcttttta gttgaaaaac gagagcaaaa
    35761 ctgttagact tacagtcgtg aggttgagat ttccctttat tgatgtgaaa ttcgaatagt
    35821 taatttcttt ttttatcgta tatgggtcgt tttaagtcta cattggacgt aaagtgtcca
    35881 actaaggaga tcgctatgtt ggatgaaata aaaatgattg aatctgttgc accagatatt
    35941 atcgaagtgt tgcaagaacg ctataaaatt ttacgaaata tctattggat gcagccgatt
    36001 ggacgtcgaa gtttatcgga aagtatgggg atcacagaac gagtattgcg aacagaaaca
    36061 gatcttttga aaaatctgaa tttgatcgac acctcaaaaa gcggtatgac gctgacaact
    36121 aagggcgaag aagtttatca aagccttgaa aacttcatgg atcagctttt aggtacgcat
    36181 caaaccgaac aacagttggc agaatatttt ggtaaccaac gctgtatcgt tgtttcagga
    36241 aacagcgaag agcagaccaa agtggctgat gcattcggcg aagcgctgag cgaagcccta
    36301 gaccgactac ttccagaagg cgagaatatc atcgctgtga tgggaggaac tactatggct
    36361 gtggtagccg aacaattgtc taatctggaa aataagaaac ggcataacct gtttgtccct
    36421 gcaagaggcg ggattggtga agcgataact gtacaggcaa attctgtcag tgcaagaatg
    36481 gctgctaaag caaacggtaa ccaccgtgca ctttatgttc cggaacaatt aagtctggca
    36541 acttacaatt ctttactcaa tgagccatct atccaagaag tcttaaactt gattagtgaa
    36601 gcgaattgtg ttattcatag tattgggcgt gcattacaca tggcagcacg tcgtaagatg
    36661 actgaaaaag aattagtcat gctgaaacaa gcaaatgctg ttgcggaatc ttttggctac
    36721 tttttcaatg aaaaaggtga agtcgtttat aaggtcccta gaatcggcat agaattgcaa
    36781 gatttggaaa aagtcccgat catcatggca atcgcgggcg gaaaatcaaa agccaaagca
    36841 attcgagctt atatgaaaaa tgcaccaaaa caaacgtggc tcatcactga tgaagctgct
    36901 gcaaatgaga ttttaaaagg ggtaaccctt taaaataaat atttttttga ttttcataag
    36961 gaggaaatct taaatgacag ttaaagtagg tattaatggt tttggacgta ttggacgtct
    37021 agcattccgt cgtatccaag atgtagatgg aatcgaagta gtagcaatca acgacttgac
    37081 agatgctaaa atgttggcac acttgttaaa atacgacaca actcaaggac gtttcaacgg
    37141 aacagttgaa gttcatgaag gttcattcaa cgtaaacgga aaagaagtta aagtattagc
    37201 taaccgcaac ccagaagaat taccatgggg cgaactaggc gtagatatcg ttctagaatg
    37261 tactggtttc ttcacttcta aatcagcagc tgaaaaacat ttaacagctg gtgctaaacg
    37321 cgttgttatc tctgctcctg gcggaaacga tgttccaaca atcgtttata acacaaacca
    37381 cgaaacatta actggtaaag aaacagttat ctcaggtgct tcatgtacta ctaactgttt
    37441 agctcctatg gctaaaacat tgaacgacaa atttggtgtt gttgaaggat taatgacaac
    37501 tatccacgct tacacaggtg accaaatgac tctagacgga cctcatccta aaggtgactt
    37561 ccgccgtgca cgcgctgctg ctgcaaacat cgttcctaac tcaacaggtg ctgctaaagc
    37621 tatcggttta gtaattcctg aattgaacgg taaattagac ggagctgctc aacgtgttcc
    37681 tgtaccaaca ggttcattaa cagaattagt aacagttctt gaaaaagaag taactgttga
    37741 cgaaatcaat gcagcaatga aagaagcttc aaacgaatct tacggataca acacagacga
    37801 aatcgtttct tcagatatcg ttggtatgac ttacggttca ttatttgatg ctacacaaac
    37861 taaagtaatg acagttggcg acaaacaatt agttaaaact gttgcttggt acgacaacga
    37921 aatgtcatac actgcacaat tagtacgtac tttagaatac ttcgctaact tataagatag
    37981 acaaacgtac aagtgtaaat tatagacatg tacatttaaa agcggggaag caatcgcgct
    38041 tcttccgctt tttttgataa taagtttatt caggaggaaa taaaatggct aagaaaacag
    38101 tgaaagatat cgatttaaaa gacaaaaaag ttcttgtccg tgttgacttc aacgttcctt
    38161 tgaaagacgg cgtgatcact gacgacactc gtatcaaagc agcattgcca acaatcaact
    38221 acgttctaga acaaggcgga aaagcaatcc ttttctctca ccttggacgt gtaaaaacag
    38281 aagaagataa agaaggaaaa tctttagctc cagttgcaaa acgtctaggc gaattattag
    38341 gtaaagaagt aacattcgta cctgaaactc gcggagaaca attagaagaa gcaatccgca
    38401 acatgaatga cggcgacgtt gtcgtattcg aaaatacacg atttgaagat attgacggta
    38461 aaaaagaaag caaaaatgat acagaactag gtaaatactg ggcttcatta ggcgatgtat
    38521 ttgttaacga tgctttcggt acagctcacc gtgcacatgc ttctaacgta ggtatcgctt
    38581 caactggtat cccaacagtt gctggattct tgatggaaaa agaaatcaag ttcatcggtg
    38641 aagcagtaga agaaccaaaa cgaccaatga ttgcaatcct tggtggcgca aaagtttctg
    38701 ataaaatcgg cgtaatcgaa aacttgattc ctaaagcaga taaaatcttg atcggtggcg
    38761 gaatgactta cacattctac gaagcaaaag gaatcaagat cggtaactct ctagtagaag
    38821 ctgataaagt ggaactagca aaagaattaa tcgaaaaagc aggcgacaaa cttgtcttgc
    38881 caatcgacaa tgtctgtgca ccagaatttt caaatgatgt tgaaacacaa gtaatcgaag
    38941 gagacattcc tgatggtttg atggctttag acatcggccc agcatcagta aaattatttg
    39001 ctgatacatt acaaggtgca aaaacagttg tttggaacgg acctatgggt gtatttgaaa
    39061 tgagtaactt tgctaaaggt acaatcggtg tttgtgaagc aatcgctaac ttagaaggcg
    39121 caacaacaat catcggtggc ggagactcag ccgcagcagc agaacaatta ggatttgctg
    39181 acaaattcac acacatctca acaggtggcg gcgcaagctt ggaattgctt gaaggtaaag
    39241 aattaccagg actagcagca atcaacgata aataaacgac caagtttgct caattctgac
    39301 gagtaatcga tcaaaaggtg aagggagttt tcatgactcc ctagccattt ttcaataaat
    39361 taaggagtgt gttcctcaat gcgtaaacct attatcgcag gtaactggaa aatgaacaaa
    39421 actgcatcag aagcaaaaga atttgctgaa gcagtaaaaa acaaaatccc ttcaaatgac
    39481 aaagttgatt cagtcatcgg atctcctgca ttattcttac aagaattaat gacatcagct
    39541 gaaggtacag aactaaaaat ctcagcacaa aactgctact gggaaaactc aggtgcgttc
    39601 actggtgaaa cttcaccagc agctcttgct gatctaggtg ttcattatgt gatcatcgga
    39661 cattcagaac gtcgtgaata tttccatgaa acagacgaag acatcaataa aaaagcgaaa
    39721 gctatctttg caaatggtat gacaccaatc ctatgctgtg gcgaatcttt agaaacctat
    39781 gaagcaggca aaactgctga atggatcgaa ggacaaatta cagctggttt agctgatctt
    39841 tcagctgaac aagtgtcaaa catggttatt gcttatgaac caatctgggc aatcggtact
    39901 ggtaaatcag ctgacgcaca aatcgctgac gaaatttgtg gagttgtacg tcaaacagtt
    39961 gaaaaacttt atggaaaaga agtatcagaa gcagttcgta tccaatacgg cggttctgta
    40021 aaaccagaaa atattgctga atacatggca aaagaaaatg ttgacggtgc tttagtcggt
    40081 ggcgcaagct tgcaagcaga ttcattcttg tcattgttag acgctgtaaa ataattgttg
    40141 aaaatttgcg aaaaatgcaa gaaatgtttg cactaatgag caaaattaac taaaatcgta
    40201 tatgagggaa atcccttagg aaaaaacaaa ctcaaaggag agacaaaaca tgtcaattat
    40261 tactgatgtt tacgctcgcg agatcttaga ctcacgtggt aacccaacaa tcgaagtaga
    40321 agtatacaca gaaagcggag cttttggccg cggaatggtt ccttcaggtg cttcaactgg
    40381 tgaatacgaa gcagttgaat tacgtgacgg agacaaagct cgttatggcg gtaaaggtgt
    40441 aactaaagct gtcgacaacg taaacaacat cattgctgaa gcaatcattg gctacgatgt
    40501 tcgtgaccaa atggctatcg acaaagctat gatcgcttta gacggaactc ctaacaaagg
    40561 taaattaggt gcgaacgcta ttttaggtgt ttctatcgct gttgcacgtg cagctgctga
    40621 ctacctagaa gtacctttat accactacct aggcggattc aacacaaaag tattgccaac
    40681 accaatgatg aacatcatca acggcggatc acatgctgat aactcaatcg acttccaaga
    40741 attcatgatc atgcctgttg gcgctccaac attcaaagaa gctctacgta tgggtgctga
    40801 agtattccac gcattagctt caatcttgaa agctcgcgga ttagcaactt ctgttggtga
    40861 cgaaggtgga tttgctccaa accttggttc aaacgaagaa ggttttgaag taatcatcga
    40921 agcaatcgaa aaagctggct atgtacctgg taaagacgtt gttcttgcta tggatgctgc
    40981 ttcttcagaa ttctacgaca aagaaaaagg tgtttacgta ctagctgatt caggcgaagg
    41041 cgaaaaaaca actgacgaaa tgatcaaatt ctacgaagaa ttagtttcta aatatccaat
    41101 catctcaatc gaagacggat tagacgaaaa cgactgggac ggattcaaga aattaactga
    41161 cgtattaggc gacaaagttc aattagttgg tgacgacttg ttcgtaacaa acactcaaaa
    41221 attatctgaa ggtatcgaaa aaggaattgc taactcaatc cttatcaaag tgaaccaaat
    41281 cggtacatta acagaaactt tcgaagctat cgaaatggct aaagaagctg gctacacagc
    41341 agttgtatct caccgttctg gtgaaacaga agattcaaca atctctgata tcgctgttgc
    41401 aacaaacgct ggccaaatca aaactggttc tctatcacgt actgaccgta ttgctaaata
    41461 caaccaatta ctacgtatcg aagaccaact tggtgaagtt gcagaataca aaggtttgaa
    41521 atctttctac aacttaaaaa acaaataatt cttttctaat gaagatacat gatcgactat
    41581 tgaaagatag tcgagtatga tcacttagtt aagtaatcga actctctact tgctgaaata
    41641 tcagcaggta gaga
//
LOCUS       DACEXQ010000003        40298 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.003, whole genome shotgun sequence.
ACCESSION   DACEXQ010000003 DACEXQ010000000
VERSION     DACEXQ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.003"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..691)
                     /locus_tag="IXH04_000105"
     CDS             complement(<1..691)
                     /locus_tag="IXH04_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1850746.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATE"
     gene            complement(871..1215)
                     /locus_tag="IXH04_000106"
     CDS             complement(871..1215)
                     /locus_tag="IXH04_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ1850747.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(1208..1642)
                     /locus_tag="IXH04_000107"
     CDS             complement(1208..1642)
                     /locus_tag="IXH04_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAQ1850748.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            1780..2517
                     /locus_tag="IXH04_000108"
     CDS             1780..2517
                     /locus_tag="IXH04_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1850749.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            2510..3721
                     /locus_tag="IXH04_000109"
     CDS             2510..3721
                     /locus_tag="IXH04_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1850750.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            3928..4707
                     /locus_tag="IXH04_000110"
     CDS             3928..4707
                     /locus_tag="IXH04_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAQ1850751.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            4777..5439
                     /gene="trmB"
                     /locus_tag="IXH04_000111"
     CDS             4777..5439
                     /gene="trmB"
                     /locus_tag="IXH04_000111"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAQ1850752.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(5537..7291)
                     /gene="ade"
                     /locus_tag="IXH04_000112"
     CDS             complement(5537..7291)
                     /gene="ade"
                     /locus_tag="IXH04_000112"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAQ1850753.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            7423..8352
                     /gene="cpaF"
                     /locus_tag="IXH04_000113"
     CDS             7423..8352
                     /gene="cpaF"
                     /locus_tag="IXH04_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAQ1850754.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            8309..9358
                     /locus_tag="IXH04_000114"
     CDS             8309..9358
                     /locus_tag="IXH04_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAQ1850755.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            9355..9633
                     /locus_tag="IXH04_000115"
     CDS             9355..9633
                     /locus_tag="IXH04_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAQ1850756.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            9608..10078
                     /locus_tag="IXH04_000116"
     CDS             9608..10078
                     /locus_tag="IXH04_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAQ1850757.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            10095..10397
                     /locus_tag="IXH04_000117"
     CDS             10095..10397
                     /locus_tag="IXH04_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1850758.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            10414..10785
                     /locus_tag="IXH04_000118"
     CDS             10414..10785
                     /locus_tag="IXH04_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAQ1850759.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            10757..11116
                     /locus_tag="IXH04_000119"
     CDS             10757..11116
                     /locus_tag="IXH04_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1850760.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            11288..12295
                     /locus_tag="IXH04_000120"
     CDS             11288..12295
                     /locus_tag="IXH04_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1850761.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            12320..13504
                     /locus_tag="IXH04_000121"
     CDS             12320..13504
                     /locus_tag="IXH04_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAQ1850762.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(13941..15524)
                     /locus_tag="IXH04_000122"
     CDS             complement(13941..15524)
                     /locus_tag="IXH04_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1850763.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(16102..16245)
                     /locus_tag="IXH04_000123"
     CDS             complement(16102..16245)
                     /locus_tag="IXH04_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAQ1850764.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(16468..16947)
                     /locus_tag="IXH04_000124"
     CDS             complement(16468..16947)
                     /locus_tag="IXH04_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ1850765.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(17020..17304)
                     /locus_tag="IXH04_000125"
     CDS             complement(17020..17304)
                     /locus_tag="IXH04_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1850766.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(17382..17576)
                     /gene="ssrS"
                     /locus_tag="IXH04_000126"
     ncRNA           complement(17382..17576)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IXH04_000126"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(17648..18934)
                     /locus_tag="IXH04_000127"
     CDS             complement(17648..18934)
                     /locus_tag="IXH04_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAQ1850767.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            19072..19743
                     /locus_tag="IXH04_000128"
     CDS             19072..19743
                     /locus_tag="IXH04_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAQ1850768.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            19709..20197
                     /locus_tag="IXH04_000129"
     CDS             19709..20197
                     /locus_tag="IXH04_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAQ1850769.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            20210..21157
                     /gene="prmA"
                     /locus_tag="IXH04_000130"
     CDS             20210..21157
                     /gene="prmA"
                     /locus_tag="IXH04_000130"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAQ1850770.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            21158..21922
                     /locus_tag="IXH04_000131"
     CDS             21158..21922
                     /locus_tag="IXH04_000131"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAQ1850771.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            22155..24368
                     /locus_tag="IXH04_000132"
     CDS             22155..24368
                     /locus_tag="IXH04_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAQ1850772.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            24379..24825
                     /locus_tag="IXH04_000133"
     CDS             24379..24825
                     /locus_tag="IXH04_000133"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAQ1850773.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            24996..26255
                     /locus_tag="IXH04_000134"
     CDS             24996..26255
                     /locus_tag="IXH04_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAQ1850774.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(26344..27291)
                     /locus_tag="IXH04_000135"
     CDS             complement(26344..27291)
                     /locus_tag="IXH04_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ1850775.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            27685..28977
                     /locus_tag="IXH04_000136"
     CDS             27685..28977
                     /locus_tag="IXH04_000136"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAQ1850776.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            28992..30761
                     /gene="aspS"
                     /locus_tag="IXH04_000137"
     CDS             28992..30761
                     /gene="aspS"
                     /locus_tag="IXH04_000137"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAQ1850777.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            30870..31745
                     /locus_tag="IXH04_000138"
     CDS             30870..31745
                     /locus_tag="IXH04_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1850778.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            32011..33894
                     /locus_tag="IXH04_000139"
     CDS             32011..33894
                     /locus_tag="IXH04_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAQ1850779.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(33969..34253)
                     /locus_tag="IXH04_000140"
     CDS             complement(33969..34253)
                     /locus_tag="IXH04_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ1850780.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(34243..34977)
                     /locus_tag="IXH04_000141"
     CDS             complement(34243..34977)
                     /locus_tag="IXH04_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAQ1850781.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            35106..35729
                     /locus_tag="IXH04_000142"
     CDS             35106..35729
                     /locus_tag="IXH04_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAQ1850782.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            35921..37042
                     /locus_tag="IXH04_000143"
     CDS             35921..37042
                     /locus_tag="IXH04_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAQ1850783.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            37049..40177
                     /locus_tag="IXH04_000144"
     CDS             37049..40177
                     /locus_tag="IXH04_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAQ1850784.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
BASE COUNT        12933 a         7164 c         8479 g        11722 t
ORIGIN      
        1 attctgtcgc ataagaagta ggatttgttg tagtaataac atcggagatc tcaccgtctt
       61 tcaatttgaa agctgcttct tttacttctg caggaatagt tgttgtcgtt gagtcgaatt
      121 taactttacc gccatcttct tttgtctctg tatcagttga tttgtctttg gctaatttag
      181 agaaatcatc tccatcatcc gctgattttt tcacatcttt tgcttcatct tcacttgata
      241 atttgatgat ttgtgcttct acttctggat ggaaagattt ccaagcagtt ttcaaatcgt
      301 catctgtaat atctacgtga gctttcaagc cagcttcaaa agctaaattg ttacgaatat
      361 attctttata agtatctttc gtatatccag cagcttctaa ttgtgattca aaagtatcac
      421 ctaatgattt cgcttgtttg tcgtattctg catccacttg cttatcggtt actttatcgc
      481 catatttgtt attgaatact ttataaatga tcatccgttg tactaaggat tggttcgatg
      541 attcaagttt tgcttcatca tagaaatcag atacagtgat cgttccgcct ttcatcgttg
      601 cgatatcttt attagtatcg cctgaacaag ctgctagagt taaaacagcc aacgcactag
      661 tagctgccaa aataattgat ttttttttca ttaaaatttg cactcctgta attagttatt
      721 atttttatat attaactata ccatatccca tttcctttaa aaacgttgtc gtggtagttt
      781 caaacaaaat tcaaaatttc atcacattcc taggaaatac gcaaactttt ctcacttata
      841 tagttaaatc tgttgagaaa ataatcatta ttatatttgt tcaggatact ctggcagatc
      901 agcttttagt ttttctactt gttcattgat ttgtgtgata cgtggttcgg cttggaattt
      961 aaaatcttcg atatcttttt tgattgcttc ttggaaagca ggaatcacag attcagctgt
     1021 ttcttttgtt tcgatcaatg cgcttttaaa atgattcagg ctgttattta aatcatttgt
     1081 cagagttgtt gcttcttcca attcttcgat cagtttcttt cttgtttcat tcccgctcct
     1141 gggcgccaat aacagacctc cgacagtgcc agcagcagct ccaaataaca gtcctttgaa
     1201 aaagttttta atcatcgcag gacacctgct ttacgattgt ttcttgaata gcttttagtt
     1261 cttccgatcc ataactatct tgatgattga caaagtgaat agaaaaatca tcctctttgc
     1321 tgtagcgagg gactaaatgg atatgtgaat gaaaaacaga ttgataggct agttcacggt
     1381 tgttattcaa gatattaaga cctttcattt ctggaaaagc tttttccaat gcttgagcaa
     1441 tcttaggcac acgcgaaaaa acctctcctg caagttcggg atcatattca aaaatatctg
     1501 ctacatgacg tttaggcaca actaatgtgt gtcctttcgt tgcttgagag atgtctaaaa
     1561 aagcatagac cttgtcgtct tcatatattt tatagcttgg gatttcttga ttgatgattt
     1621 tgcaaaaaat acagttttcc atataaaaca tcctttctat aaagaataaa tacttcagct
     1681 taactttacc ataagttaaa gctattgtaa ggaaaaaaac aaaataccgt cattttcttc
     1741 gtggtataat aaataaaaat aaatggcgga ggaaaaagaa tgagtttaat tatcgatcat
     1801 gtcacagggg ggtacggaca tctccctgtg ttaaaagata tcagtttcga tgtacagcct
     1861 ggagagatag tagggttgat tggactgaac ggagcaggga aaagtacgac catcaagcac
     1921 attatcggtc tgctggaagc ttcaaaagga acgatagaaa tcaatcatct atccttaaag
     1981 aaaaatacgg aagaatatcg caaacaaatt gggtatattc cagagactcc ttctttatat
     2041 gaagaattga cattgagaga acatatcgaa gtcactgcga tggcctacga tatacctatg
     2101 gatatagcaa tgaaaagagc agaaaagttg ttggagactt ttcggttatc taatcgtctt
     2161 gactggttcc cagctaattt ttcaaaggga atgaaacaga aagtgatgat cttatgcgcc
     2221 tttttgatcg agccttcttt atatatcatc gacgaaccat ttttaggctt ggatccacta
     2281 gctatcaatg cattgcttga attgatggta gaaatgaaaa aagaaggagc cggtattttg
     2341 atgtctacgc atattttagc tactgcagaa aaatattgtg atcgttttgt ggtcttacat
     2401 aacggagaga tacgagcaga cggttctctt gagatgttga gaaaagaatt tcagctgccg
     2461 gattcttctt tggacgaaat ctatattgct ttaacaaagg agcaggcaca tgagtagttt
     2521 ttttcaaaaa cgtttagcaa gacatcaaaa gaaaatgatg aaatatatgc gttatgtatt
     2581 gaacgatcat tttgttttag tttgtctttt cttgattggc ggtgtgggat tatattattc
     2641 taattggttg aaaaccctgc agccaccgtt tgcaattgga gggctcataa tctctgtatt
     2701 ttggatgctc tgtttattcg tgggtaagat ggctactttt gcagaggcag cagatattgt
     2761 ttttttactt cctaaagaaa aagaaatgca aacctatttg gaacgagctt ttcgctattc
     2821 ttgcattttt ccgttgatct ttttgatatt ggcatgcgga ttggccatgc cattagtggt
     2881 ggttagtacg gagcaatcct tttcgatgtt tttcgtctat ctgatcatat tatggagttt
     2941 gaaattcagt catctgcaaa tcaagagagc agaattgttt cgactagatc aacaagttat
     3001 tcgttattgg aaattaggct ggtttttttc ttctctgatc tttttgtttc tatcatttta
     3061 ttggctaata tgggcaggaa tagcaggtgc attagtacag gcaagtctat attactggtt
     3121 tttgtggaag caacaagaaa aaggattgga ttgggagaaa atggtgcatg tagaagaaca
     3181 taggttgcat cagatctatc aatttatcaa tttattcaca gatgttcctg aaattacggc
     3241 aaagatcaaa agaagacgct atttggatcc tatattgact catattaaaa gagaatcaaa
     3301 aaacacttat ctttacttgt ttgcaagaag attggcaaga ggttctgatt ttagtggtct
     3361 ttatttccga ttagtaatta taggtgggtt attgattgcc ggagtagtcg acttttattt
     3421 tagtctgatt atcggtttct tatttttata tttgatcggt ttccaactgt tacctttagc
     3481 gaaccagttc cgctatatgg ttctcgttca gctttatcca atccataaga atcagaagaa
     3541 gcaggcgatt cagtatctac tcacatgggt attaattatc gcgtcggtta tttttggttt
     3601 gattgctgca gtcgtattag atgggcagca gcgatgggtt ccgctagcgg cttatttgat
     3661 tgttactatg ctctttgtta aactatatgt accaactcgt ttgaagaaga tgtcagactg
     3721 aaaacaaagc tgtgtagggt aaaaatgaga atgaaaaata gagcgttgac agtaagaatg
     3781 ctgttcgttc tatttttttt gagaaaagct ggttttttga atggcaatta aatgaaaaag
     3841 aacgtatatg tgcttgacgt gtagtgttca ggcgactaga ataataagta ctgcaaaaca
     3901 aagagtaaga ggacgggaga atgcatgatg gagtttcagt tagaccaaga gtggcggtta
     3961 cgtcccatca aaggcgacac aggaaaaaca tacataggca tgcgtgatga ggacaaagtc
     4021 tttattaaac gaaacacgac tccgatgttg gctgctcttt caagggaagg aattacacca
     4081 aaacttgttt ggacaaaacg aacggggaat ggcgatactt tgacagcaca agagtggctg
     4141 gaaggtcgct tgctgactcc taatgagatt ggtcaaagaa acgacgtgat tgatgttttg
     4201 taccatttac atcattcaaa ttctcttaaa accatgctga cccgtatcgg tggtcgtgtc
     4261 atgacgccgg agaagatgct gcaaaaatat gaaaaagaat taccggaaga actccagaaa
     4321 aatcaatttc tgcaaattgt ccatcgtttt cttcgtgagc agatcccgtc aatagataag
     4381 gaaaacttat cagtagtaca cggagatgta aaccatcgta attggttagt atgtaaaaat
     4441 tatttatatc tagtcgattg ggattcaatc atgtttgcag accctgcggt ggatattgca
     4501 acgattttag gaaattatgt gccactgtct agctggaatc agtggctgat cagttatggc
     4561 atacgcccga ctgacgaaat gcttgaaaaa tttcattggt atgcggccat gaatttgctc
     4621 caagaaatca ccagatattg caaacgtgaa gatcatcgac ggatgaacga agaaatttta
     4681 caattaaaac gaattttcag tggctgaggt ttggcccaaa ctctgcggag atttggccag
     4741 acctttttaa ttagaggaaa ggaatggaga taaataatgc gtgtccgaaa tcgtccggga
     4801 gcagcggaaa tgttagcggc tcacccgaat tttgtaatta gtgatccaac attgtggaag
     4861 ggaaaatgga atgaattgtt tgaaaacgat catccaatcc acattgagat tggtatgggg
     4921 aaaggccagt tcatcactgg aatggcaaaa gcccatcctg aaatcaatta tattggagta
     4981 gaaatgcagg tcagtgttgt ttcgattgca ctagacaagt tgatcgaaca accgcttcct
     5041 aacctgaaac ttctacatgt ggatggatca gcgttgacag agtatttcgc tgacagcgaa
     5101 gtagatcaaa tctatctgaa tttttccgat ccatggccaa aaaaacgtca tgaaaaacgg
     5161 cgattgacat ataagacttt tcttgctgta gatgaacaga ttcttcgtcc aaatggagaa
     5221 atccatttca aaacagataa tcaaggattg tttgagtatt ctctggcaag tttttctcaa
     5281 tatggcatga tcctcaaaca ggtatggtta gatttacatc aaagtcaatt tgaaggcaat
     5341 attatgacgg agtatgaaga aaaattttct tcaaaaggtc aacgtattta tcgagtagaa
     5401 gctcaatttc aagataagac caaaaagaaa agtctgtgaa actaactttc tgctttcata
     5461 aaaacattaa tatccataaa taaatcccag aaaatgaaca ttacagatgt aatgtatttt
     5521 ctgggattta tttgatttaa aatgtattta tttcatcggt ttttccacaa ggtggaatca
     5581 attggaagcg agaaaatgaa aataatcctt tgtcggtgat cttcaattcg ggtatgacag
     5641 ataatgttaa gaaagaaagt gtcaaaaaag gatcaaaggt tttagctgct cctagttgat
     5701 gtgctttagt tattaattca ttcaactgtt catagacgat cgaaaaaggt tcctgtgtca
     5761 tgattccgcc tataggaaga gaaacacaag ctatttcctg ctgtttgttg acaacggtca
     5821 tcccgccacc tttttgaatc aaaagattag cagcatacag catatcctcg tcgtttgtcc
     5881 caacgacaac tagattatgc gagtcatggg caatcgttgt cgctatcgct ccttgtttca
     5941 gctgaaatcc tttcactatg cctaatccaa tgtttcccgt ttgatggtga cgctcgatca
     6001 ccgcaatctt taattggtct ttttcgattg aaggaatgaa tcgttcgcct tgtcggtcca
     6061 ctttttctac caaatcttta gtcactagac tattcgggat aatttcgatc acatgggctt
     6121 gtgaagaaga aagaagcagc tctaacgaat gtggatttaa tggttgagcg tttagttttg
     6181 gcagacaaca cgtatatgaa tttgaatgat cagcaaataa atggtcttgg agttgcccag
     6241 attgaacaac taatgttccc ttatagaaaa cttcatgaat tggcagcttg gtaagatcgt
     6301 ctgttaagaa gaaatcagct tgatacccag gggcaatcgc acctagtgtt ttcaaatcaa
     6361 aacattctgc tgcattcaat gttgccatct gaatggcttg taatggatcg attccaagtg
     6421 caatagcttg acggacgata tgatcgattg atccttcttt tactaaatca ttgatcagtt
     6481 tatcatcggt gacaaagaag catcttcttg aattttcagg tgtgatcgcc ggtagtagcg
     6541 atgccagatc ttttgcaacg gtcccttctc gaaccattaa atacatacca agatccaaac
     6601 gttctttagc ttcttttgca gttgtagctt catgatccgt ccgaatacct gctgcaggat
     6661 aaacatttaa ctcttccatg ccgataccag ctgcatgccc gtcaattttt ttcttatgct
     6721 ggtgcatcaa ttggatcttt tctaggatgt ctgtttcatt atttgctact gcttgatagt
     6781 tcatgacttc agctaagcct aaaacttcgg gtttttgtaa aaaaggggct aatttttttg
     6841 catttaaatt tgcaccattg gtctcaaaag gggttacagg tacacaagaa ggtagcataa
     6901 aaaagatatt catgagtgtt tgtcttgcat cttctatcat aaactcaaga cctgtactcc
     6961 cagcaatgtt tcctatttca tggggatctg tcactacagt agtaactcca tgaagcaagg
     7021 atacttttga aaattcgctt ggtgataaaa gtgagctttc gatatgcaca tggccatcga
     7081 tgaatcctgg cactagatat tgcccattag cattatgtac gctttttcct tcgtattcac
     7141 cgattcctac gatcattcct tcgcagattg ctacatcttt ttccatgatt tctttcgtaa
     7201 agacgttgac gatcttagca tgcttgatca ctaggtcagc aggctttttt ttacttgcta
     7261 cttcgatata tttccttaat gttgtttcca atggagtgcc tcccaagata aatttttata
     7321 agtataacat aaattcggtt tttgccaata tttttttgta aattcatttt atttgaaaca
     7381 aaaagatcaa aaagacgtat gttttttgtg gaggggagaa taatggaaat aaaacgatta
     7441 tcggatcaaa tgataagtga aagttttgcc aaacatgccc aagacgtata tatattgcca
     7501 gcttcagaag tattttacat ctattttcgc tatggaaaaa aacgaacatt ctggaagaaa
     7561 gtctcttcgg cagatggaca gcaactgatc tcaagattta agtatttggg acagatggat
     7621 atcggagaaa aaagaaaagc tcagttaggt gcatttactt attctttaga aaaacaggaa
     7681 gttcgtctgc ggttgtcttc tgtaggagat taccttcaac gagagagttt agtgattcgg
     7741 ctattatacg gtacttttga aacaccccat cgttgcttta gttctcgaga ttttgcaact
     7801 attttacaga aaaccgtcag acgtggatta taccttttca gcggaccagt cggatcaggc
     7861 aagacttctc taatgtatcg gttggctttg gaagaaaaat tgcaagtgat tactatcgaa
     7921 gatcctgtag agatagaaga accgtttttt ctgcagctac agatcaatcc gaaaatagca
     7981 cagacttatg atacactttt gagactcgca ttacggcatc gtccagactt gctaatcatt
     8041 ggcgagataa gagatgaatc aactgcacat gctgcggtaa gagcagcact aacagggcac
     8101 cgggtttttg caaccgtaca tgcacgcaat cttgaaggta cattggtcag aatccatgaa
     8161 ctgacaaaga aaaaagaaga gctatctgaa tgtttagctg gtgtcattta tcaagaactt
     8221 ttggaagatg ccaatcacca tccttctgtt ttatggagtt atcaatttat ggaggaagaa
     8281 tcagtaaaaa aatcatggga ggaaagttat gaagaagctc aggaaaatat ttggcagtca
     8341 gaactattct aaaggattaa caagaagcca gcaaaaccag tttgcacaaa ccatgggaga
     8401 actgctttta tctggtttta gtctgcaaga atccctttat ttattactca cgatccgttc
     8461 gtttccacct gttattttgg caaatatcca acatcagcta aaggaagggc aagcgtttag
     8521 tgaggtactt tcgcaaatag gatatggagc agagcaactg gtacagatcg agttagctga
     8581 acgtcatggg aatctgccga caactttgtt ggaaattgcc aaacagtttc ggcttgttga
     8641 aggatttcgc aatgatttga aaaagctgct tgcatatccg atgctattgc tcaccttttt
     8701 gctaggtatc ttgtttagta tgcggttgtt cgttttgcct cagcttattg gttcaggaat
     8761 ggtcatgcaa gagcattggg gtattcagct gatacaagtc tttcattggt atgttctact
     8821 attcatccta gcggttactc tattggcttt actagtaagg tatcgtctga tgaagttgtc
     8881 tcctgttgat cagagtgaat ggataagcag acaattattg attggaccga tatattcctt
     8941 gtatcaatcg gcttatttgt cactagagat tggaaagctt tttcatgaag gtttcgaatt
     9001 aaggcaaatc atagtctgct taaaggaaac gaaacaagaa agtctgattc agcatcttgc
     9061 ctctaagttg attcaaggat tggaggcagg tatgtcattg gctgagcagt tccagaatta
     9121 tcgttttcta tcagaagaat tcagtaaaat tgttttacag ggagaggcaa agggaagttt
     9181 aggtaaagaa ctgatttttt acagcgaatg gacaagaaaa cagcttttca aacgtcttca
     9241 tcactggatg cattttattc agccaatcat ttttttgggc gtagcggctt tgattttatt
     9301 agtttatgcc gctgttttat tacctgttta cggaagcata gaggaggttt tgccatgaaa
     9361 aagagaaaaa tatcttatta tagcggtttt acattgatcg aaatgctaat cgtcttactg
     9421 atcatcagtg tattagtgtt gttgttcgtc cctaatttgt cgcgttatag gaatcatgta
     9481 gaccaagaaa gtcgtgaagc gatcatccag ttggtagaca cacaaaaaga gctctatgcg
     9541 cttcaaaata atggaagagt tcctactgtg gaggaattgt tgaatgaagg ctatatcaaa
     9601 cgagaacatg cagaaatcta tcagcggcct tgaaggtaag gttttagcat caggatatac
     9661 gttgtttgaa tcactcttac tgattttatt acttacaacg atcctttctt ttccagttct
     9721 cgcattttct gcatggaaga atgaattggc agtctatcaa tttttcggcc aatttgaaaa
     9781 acgaatctat tctacgcaaa aaatcgctat tgtgaatcag atccaaacag gcttttattg
     9841 gaatagtgaa gagaatcaaa tcatttttca tgttccgaat ccagataaaa taaattggga
     9901 agtattagat attcctgatg agattacctt gaaacgccat gcttctatta cttttgctgc
     9961 tagaaccggg aatgaaagtt cactaaaagc ttatcaattt tattgggaag aaaaagagcg
    10021 gacgattacg tatcaattcc agatagggag tggtcgctat acaaaaagaa ttgaataaaa
    10081 caggatttgt tttgttggag tcccttacag cgcttatgat cgcttctgtc tgtgtctact
    10141 tagtgagctt tggccagctg tttttgcttc aggcagaaaa gaaaagtggg gaggaactgc
    10201 tatttattcg aatgatttat gaagatatca agacgcagcg gatgtatcac aaaagaccag
    10261 agcgaaggga agaaatgatc ccatacgata tccaaattgt cgataataat ggagtaatcg
    10321 aaaaagtgtt tgtcaatatg gagaaagaag cgatcagtat tgaaaaaaca agtaattcca
    10381 gcatttacgc tgattgaatg tctaattgga ctgatgatat tgagcagtct attgctcaca
    10441 ttcaatcttt tgattcagca aaccatccaa gtaagtgaat ttttgcagcg taaagatcaa
    10501 aaagagtggc taatattttt agcacaatta gaagaagaac tcaagagcag tcacaatgtc
    10561 tctgtgaaaa atcagcaact gcactacacg atcgaggata aacagtatat catcgaaaga
    10621 tatcaggcaa tgatacgtaa aagaagaact tccggcggac atcaacctat gttaacaagt
    10681 atcactgagc ttcaattatt ggaaaaagaa cacacaattt ctttttatgt acactttgaa
    10741 aatggagaag atggatatgc catatggaca aaaaacgatc agtagaaaat gttataaggg
    10801 ctctatttta ttttctgtct tgcttttatt tcttttatgt acgtttttat tgatggcagc
    10861 attagaaagc taccgtttgt caatggatct taatatacga atcaaaaatt attatatagc
    10921 aaaaataatg aagcagatgg ctttatcgga acagtctacg cttgccgaaa gttcggaagg
    10981 agttttttac tacacaactg gatctgtaac atatcagtgg gtacaacagt ccctattctt
    11041 agaggtggaa gtctcaccgt ttatctttcg gttcacagaa gaagtaaaaa atgatacaga
    11101 tactagtaca gaatgatgga atgttcgtga aaacgccctc ttttctgtta taacactgtt
    11161 tttaataaaa aaatgtttgc cgtttcacaa ataaaaaaca gtgaaaacga ttgaaatttt
    11221 gtcaattgtt cgatagaatt aaacaggtga aaaaattgtt ataatatatc tagaaatgag
    11281 gtgcaccatg ttaccagaaa aaatggaaca agggtttaat caaaacctgg aagctattca
    11341 gcttttacag aatgcattgg gaacttcctt tttagaagcc tatgtcgaaa atgcagaaaa
    11401 tttaatagat gattatcaag tacgtgtggt ggatggagtg ccgacaaaag agacaacgca
    11461 acggatcaca gctctttatg aagagttgaa gaaactttct ttcgagccag aagaatggcg
    11521 cagactgtct caattacttt tattgaaggg cagccagaca gaacatttgc aggcgaacca
    11581 tcaactcact cctgatagta tcggtttttt atttgttttt cttattgaac aactatacac
    11641 aaataaaaaa gcaccggtta agattctgga tattgcggca ggaatgggca acttgttatt
    11701 aacagttctt ttaaacctat cgaatgcggg gtaccaaaca gaaggaattg gtgtggatat
    11761 tgatgataca ttgttggctg tcgcagcttc tacaagtgat ttgacacaag caaatgttca
    11821 atactttcat caagacgggt tgcaggaatt attaatagat cctgtcgatt ttgcaatcag
    11881 cgatttgcca atcggttatt atccgaatga cgaaaaagca aaagagtttt taacaagtac
    11941 cgaagaaggc catagttatg cacatcattt attgctagag caatcaatga aatatgtgaa
    12001 gccggatggt tttggtttgt ttttgatgcc gagtggattt ttagaaacgg atcaaagtga
    12061 agaaatcaaa aaatggttta aagaagaagg ttatcttcaa ggaatgattc aattgccaga
    12121 tgaattgttc cgtaataaac aatcgcaaaa aagtattttg atcttacaga aaaaaggacc
    12181 acaagcacaa caagtaaaag aagtcttgct tgtgaaactt gcttcattaa aagaaccaga
    12241 gaaggtgacg gaatttttca acgagtttaa aaactggaaa tcatctgctc tgtaatcatt
    12301 caacgagaag gagagtcata tgtctaaaac aattgcaatc aatgctggga gttcaagttt
    12361 gaaatggcaa ttatatcaaa tgccagaaga aaccgtagtt gcgaaaggga tcgtcgaacg
    12421 tatcggttta caagattcta tttttaccat caagtacggc gataatcaaa aattcgaaca
    12481 aatcatggat atcgaaaacc acgaagtagc agtaaaaatg ctgttagatc agttgattga
    12541 actgcaaatt ttaagctctt atgatgaaat caccggagta gggcaccgtg ttgttcacgg
    12601 aggagaaact tatggggatt cagtagtgat cgatgagaaa gtaatggacc gcattgctga
    12661 attagcagaa tttgctcctt tgcataatcc tgcaaactta atggggatca aagcgtttaa
    12721 aaagatttta cctgacattt tgagtgtggc tgtattcgat acatctttcc actcaacaat
    12781 gccaaagcaa aattatttat atagcttacc ggtgtcttat tatgaagatt tcaaagtaag
    12841 aaaatatggc ttccatggca caagtcatcg ttatgtgtcg gaacgtgcgg caaaattatt
    12901 gaacaaaccg atcgaagaac taaaaatcat cacttgccac ttaggaaatg gtgtatcgat
    12961 cacagcagtc gatggaggta aatccatgga tacttctatg ggattcacac cattagctgg
    13021 ggtgacaatg ggtacacgtt caggtgatat cgatccagct gttctcccat atttaatgga
    13081 aaaattagag attgatattg atgaaatgat caatgttttg aacaagaaat ctggtctgct
    13141 aggcctaaca ggattgtcta gtgatatgcg tgatcttgaa aagaactatg aaaaagaaca
    13201 tattcgttta gcttatgaca ttttcgtgga ccgcatccga aaatacatcg gcggttatgt
    13261 gacaacgatg aatggtgttg atgcaattgt ctttactgct ggtatcggcg agaatgacgg
    13321 acatgtacgt agtgaaatca tcaaaggtat gtcttggttt ggttgtgaaa tcgatccagc
    13381 gttgaatgaa ttgcgtggtg aagaagtaga tatctctaca aaagaatcaa aagtaagagt
    13441 attagtgatc ccaacagatg aggaactaat gattgcccgt gatgtggagc gtctgcgcgg
    13501 ctaagccaaa gcattcgtta ggataaaaga tgaaacgcat gcaagaggga attcttgcat
    13561 gtgttttttt tgcggagaag accgcaaaat ggtaatttaa cttcaatttc ccttcttttt
    13621 tacaggatct tcttagggaa ttttgcttgg aaaagtggtc aaaagggaaa agactggcat
    13681 tttatttatt gattttcagg aaaatcccaa tattttattt tcattcatct ttataaggga
    13741 cattacatat gtaaaaagaa aaaacatttt tagatcagcc tcgtgggctg tcgactttat
    13801 tcttcgcttt atgtttagct agaaaaccga tccgaaacat tcattgatcg aataataaaa
    13861 acttgatttt ccacctgtac tttgcctgaa tcgtgaaagc acaggtatga aaatcaagtt
    13921 ttttgttgcg aaaaaacgtc ctagtcaatc agttggccga gcggctggcg gctgatctgc
    13981 tcctgatcaa taacgagtgc ttcttggaag tgttgctgca aaaagtagcg gtcatggctg
    14041 actgctagga gcgttccttg atagtcttgc agcatttctt caatttcttc tcgagcatag
    14101 ctgtcgaggt gattggttgg ttcatccaaa atcaggacgt tgattttttc taaaaagagt
    14161 ttcagcaacg ccaaacggat tttttcacca ccggataaat ctttgatccg tttacccaca
    14221 tcgctttgat aaaagccact tttggctaat tgccgtcgag cgacttcttc gtttccagtc
    14281 aatgtgatgg cgtaagccag caaacggcta tcgggatcgg caaactgcag ctgctgcggc
    14341 aaataaccga tttgcaagct actacctaat ttgatggtgc caccgtaagg cttttcctca
    14401 ccgagtaata atttgagtaa ggttgatttt cctgcaccat tgtcgccgat gatggctaaa
    14461 tgatctccac gatagactgc aaaactgctg tctttgaata gtacttgatc agcgtactgc
    14521 tggtgcaagt tttgaatgat gaaaacttct ttgccagatt ttccaccatt agacaagctt
    14581 tgcagccgat ttttcggggg aggtggcggt ttgacgagtg tcagtttcgc caggcggcgt
    14641 tccagttctt tggctttttt gaagaaggac tcattgtctc cttcatgtgc ccactgacga
    14701 aaacgacgaa tcatgacttt caaccgctgg acttcttttt gctgcagttc gtaatttttt
    14761 gtcagctcag ccaagcgagc ttctttcaag actcgaaaac ggctgtagtt tccgggatac
    14821 tcaatcaatg caccttcttc cagttcccat gtttttgtgg tgacttgatc gagaaattgc
    14881 cgatcatgag aaacgaccag aaccgatttt tttgtattct gtaaatagtt ttccaaccag
    14941 cgaatactgt taagatctaa atgattggtc ggttcatcga gtaaaagtaa atcggcatcg
    15001 gctgctaaaa ttttggctaa agcgacttta actcgttcgc ctccgctaag ttctgttaac
    15061 cgtgcttcct gacggtgtgg aatccctaag ccttgcaaga tcgctgcaat ccggtcttct
    15121 aatacgtaac cgcctagttc ttcaaagcga tgctgcagct caccgtaacg ggccagacac
    15181 ttctctaagt ttgtatctgg ttctgtcatc aatgcttcgt agcgactcat ttgctgtttg
    15241 aggtcctgta tctcagcggc agaatggagc aagtagtggt tgacggtcgc ttcggaaact
    15301 gtaagttctt gttcgaccgt cccgatcttt aagccttttt tgcgactgat aacaccgctg
    15361 tcgacacctt ctctgccggt caagatttgt aaaaaggttg atttgccagt tccgttgata
    15421 ccgactaagg caattttttc accagcttca atttgacagg ataaatcctc aaataatgga
    15481 ttcccgccga ttgttttttt aatagaaatt gcttgtatga tcatgatctc tctccttgag
    15541 accactcaaa aaggccatgg ttatccatgg cctgtgttat ttaaactcag attatcatga
    15601 gaaaaatacc ttgagatggc cattgatttt acgtgaattc aactgttttg gacaaacttg
    15661 tcccgtcaac agcaactgta aaaccaattg catggctaaa tgcgatatgg tatcgttggt
    15721 aattttcatt acttgttatt ttagtcatgc gcatcatagt attttccctc cttcgtgtag
    15781 ttgtcttttc cagtgtacct gaaagtgtca gaaagttcaa ctagcgctga catttactta
    15841 atatttatcg atgtgtaatt ctgtcagaga gcttttttta tctgactgag ggtaatgtct
    15901 cgtggaccac atcattgtat tctgagctaa accagcgttc aggcgaaatc atatggtgtt
    15961 tgataaaaaa ctttgtctac atgacttcct tttgccattt gcttaatacc gttgcattaa
    16021 acagctcttc tctttggaaa tctcaccaac atcgcgttca ggagtatcgt tgtggttgca
    16081 atggcaagcg gtagcctttc atcaattctt gaatgattta ccgaatgtag cacttgcctt
    16141 ctcgcggtac ccgaacccga aataaaattt tagtttggcg ataaaatcgg tatccgccat
    16201 cgatacttca tattttttcg tatcagattc atcggaaact tccacattga cgaaccaata
    16261 agctttggca tgcttaggtc gttttaaacc acaccagggc gactaatcgc cctggtgttc
    16321 actttttttt attttacttg aactcattga ctgttttccc tttattttaa acattataaa
    16381 gaccgatgag tgcttgccgc gtcttttgtt tttatcacgg ttttttgtgc aaaaaaacct
    16441 gccgatagtt ggcaggtcgt ctacgtgtta agctttggtg tggtctttag ctggcaattg
    16501 gttttctaaa tcagttcgga caacaagcac atcgcaagct gcgtttctaa taacatattc
    16561 agaaacagaa ccaataaata aacgttctac agcattcaat cctgtggctc ctaacataat
    16621 caaatctact tgattatctt ctgggatttc tctggcaata atttgttttg gtgagccata
    16681 ttctacgaca gaggttacat tattcaagcc agctttcttc gcattgcttt cataatctgc
    16741 caatgtttgc tttgccattt cagtagcttg ttctgcaagc attccatcaa aggatgaaac
    16801 ggtttggaaa gaacgtgtgt caatgacatg tgctaataga agttctccgt tatttcggat
    16861 agctacattg accgcctttt taaaagctaa ttcagcttca tcagaaccat ctacggcaac
    16921 catgattttt ttgtattgtt gtaacataag cattcactcc tcaagtcaat cgtattgtac
    16981 tcttttattg tagaacactt tagggaaaaa gagaattatt tactgctttg gttcagcaat
    17041 tttttttgca aatcggatac tgaaggtggc agaaacaata attacgatca gtaaaaagac
    17101 caaggcaata aagcgttgat tgaaaaaagc tgttaaaata cccattacgc ctaaaacagc
    17161 atatgcagct ccgtaaaatt gtaggaatcc ttgattttta tcatttttgt tgatcaatac
    17221 aaaaaagata tcctgtttat taaataaata atagctaacg aataataata ctaaagtggc
    17281 gataatcatt aaaatcctaa tcatgaacaa atcctccaaa ataagcaaca aatagaaaaa
    17341 gcaatttgcc aagagcaaat tgctttattc tttaaaagat ggtaatctcc gttaatgccg
    17401 ttgtttgtcc tgtagtattg atcccgcgaa ttcaagcagg tgggtcccat ggtcgtagct
    17461 tcgaactacc aaatgataag gcatgttacc agcagtgaca caagacgggt cccaagatat
    17521 caataaaaat gttcggtcaa cacacaaaga ggacaattcg aacatcacag atttcccacc
    17581 tctatagtag catatgaatt taaaaagagc aatgagagag ctcgcagaaa cagaaccgtt
    17641 ttcttgtcta gttttttgga ggatgctttt tccactcttt tatacggtaa tactgttgac
    17701 ccagagcctg ttcatatttc cctgtatctt ttggctgata atactgagca ttcttcagtt
    17761 tatctggtaa atattgctga ttgacccaag cctgatcaaa atgatgagga tactgataac
    17821 ccacgccacg gttgagtgat ttcgcacctt tgtaatggct atctctcaaa tgatcaggaa
    17881 catctccagc tttgccctct cgaatatcag caagtgctgc atctaatgcc atgtaggcag
    17941 aattggattt tggcgacaag caaagatcga cgacgacgtc tgctagtggg atacgtgcct
    18001 caggcaatcc caatttttca gcagctaata cagcgttcac tgtacgagcc gcagcagcag
    18061 gattacctaa acctatatct tcataaccga taaccatcag acgccggcaa atacttgcca
    18121 gatcacctgc ctcaactaaa cgggcgagat aatgaagtgc agcatccaca tcacttccac
    18181 gaatcgattt ttgaaaagcc gaaataacgt cataatgagc atctccattt ttgtcatggg
    18241 taagagcttt tctttggaca cattcttcaa tgatcgacag tgtaagatga atccttcctt
    18301 ctttatctga aggagtcgat agtgtagcca gttccagccc attcaatgca ctccgcaaat
    18361 cgccgttcgt tgcacgcgaa aggtgaagaa gagcatcttc gtctaactgg attgcttgtt
    18421 gtccaaggcc gcgttcttta tcccttaaag catgttcgac tgccaactgg atgtcttgtt
    18481 ctgttaatgg tttgacttca aagatctgtg tacggctgcg gatcgctgga ttgatggtaa
    18541 tataaggatt ctcagtagtc gctccgatca agatgattcg gccactttct aaatgaggaa
    18601 gcagaaaatc ctgttttgtt ttatctaatc ggtgtacttc atccaataag aggatcactg
    18661 taccactcat ttttgcttct tccgctacaa cttgtagatc cttttttgaa tctgtagcgg
    18721 cgtttagcat gcgaaatgca taattagtag agccggcgat cgcacttgcg atactcgttt
    18781 ttccagtacc aggaggtcca tatagaatca tggaagagag catgcgagca tcgaccatgc
    18841 ggcgtatgat ttttccttcc ccgactagat gctgctgacc aacgacttca tcgatcgttc
    18901 gaggtctcat gcgataagcc aatggctgtt gcataggatt ttcctccttc ttgtttttac
    18961 gtcccttaat tatatcatat ttagcaagat gcctagaaag aacagccttc tgttgttttg
    19021 ttttcatagc ttatccaact aatattgtgt atgataaagt aggtgagaaa aatgacggat
    19081 aaacaaaaga tagatgaatt tttttggaat aaaacgacgc cagaagtggc acgggattta
    19141 ttggggatgt atttggagca tgacacacca gaaggaagat tagctggata tattgtagac
    19201 gcggaagcat acctaggtcc agaagacgag gccgcccata gctatggttt aagaagaaca
    19261 ccgcgtgtgc gggcaatgta tgaaaaacca ggtacgatct acctttacac gatgcatact
    19321 catcggattt tgaacatcat tactcagcca gaagggattc ctcaaggagt aatgatacgt
    19381 gcaatcgaac ccgccgcaat gattgatcaa atgagtaaaa atcgtggcgg aaaaactggt
    19441 ccagatatca gcaacggtcc gggaaaatta gtggaagcat tagcaattcc tcaagaactt
    19501 tatggtcagt ctatagcaga tagttcgtta cgcttagttt ttgagaaaaa aaagacacca
    19561 aaaaagatca atgctttgcc tagaatcggt atcccaaaca aaggtgtatg gacagaaaag
    19621 ccgttgcgtt ttgttgtgtc gggaaatcct tatatctctt tacaaagaaa aaaccaaatc
    19681 gagaaagatt ggggttggag gaaagaaaat gaaaaagaag gatctattaa catatttaga
    19741 tgaaataatt gaaaaacaag tgacagacta tgatgtagct ttagattggg atacaaaaaa
    19801 tcatacgatc gagatcgttg ttcgcttgtt tgcagaaaat aaagcacaat ttgagattga
    19861 tgatgcagaa gggatcgttt cgcaagaacc aattatcgaa tttgaagacg gcgtacttct
    19921 ttttaatcct cagaaatctg tttttgatga acgcaattat ctagcagtaa tcccttatga
    19981 aggaaaaaaa gggttggcaa aatcagtagc tgattcttta gtggaatatc taaatgaagt
    20041 attagctcaa gggcaaagcg atttgcttga ttttttggat gaagacgatg acgaagctgt
    20101 attcgagctt cactgggata atcagcaact agcagaatta gtagaagtaa aacagcaaaa
    20161 tgaagcagat acttatttac cgtacccaag ttattaagga gtgacagata tgaaatggaa
    20221 tgaagtaaaa gtagaaactg aaagtgaagc agtcgaagca gttgctaata ttttgatgga
    20281 ggcaggggcg agtggtgtcg cgattgaaga ttctttagat gttgaacatt ttcagtcgga
    20341 tcaatatggt gagattttag ataaaaatga ttttacatcg ctgaaagaag gcgcactagt
    20401 catggcttat tttccggaaa cgactttctt accggaaatc ttgcctttta tcaaagatcg
    20461 tatcacacaa ttacctgagt ttggtctagc tattggaaaa aataaagtaa cagttagtga
    20521 agtagaggaa agcaactggg caacagcctg gaagaaatac tatcatccgg tgcgtattac
    20581 gcgaatgctg acggttgcgc caagctggga aaactaccag acagaagatc ctctagaaaa
    20641 aattatcaca ctagatcctg gtatggcatt tggcacaggc actcatccca cgacttcctt
    20701 gacattgaca gcactagaaa cagtcttgcg tggtggtgaa acattgctag atgtgggaac
    20761 tgggtcgggc gtgctaagta ttgcagcaaa atatcttggt gcaaaagatg tctatgcctt
    20821 tgatttagac gaagtagccg ttcgttcggc gaaagaaaat atggatatga atgaaatagc
    20881 caaagatgtc catgtatcag ctaacgatct tttgacagga atcgagattg aaagtgatgt
    20941 aatcgttgcg aacattttag cagatatcat cttattgatg atcccagatg cttggcgttt
    21001 actgaagcaa acaggcacgc tgatcgtttc aggaatcatt gaagagaaga agcaaatggt
    21061 tctggatgcc atgttagaac aagggtttat cgtggatcaa attttccagc aaaaagactg
    21121 gtatgcaatt attttgaaaa aaccagaggt ggaatagatg cagcggtatt ttttaaaaga
    21181 atcatatgaa tcaaaagact attttgtttt agaaggggaa aactatcacc acattgtacg
    21241 ggttatgagg atggaacccg aggatgaagt ttttttggca ttcaatgatc aaatagccat
    21301 tgttgcagaa atcacggaga ttactgaaga ggctgtttat ctgaaagaaa ttaaaaaaga
    21361 agaaaatcaa aaggaactgc caagttcgat taccatagcg agtggctatc ctaaagggga
    21421 aaagctggat tggatcgttc agaaatccac tgaattagga gcgcaagctt ttcttggttt
    21481 cccggcatca gcctctgtcg ttaaatggga tgcaaaaaaa cgtatgaaac gacaacaacg
    21541 gcttgaaaag attgctcaag aggcagcgga acaatcccat cggcagatcg tgccatcagt
    21601 agagttattg gaaacgaaac aagatttatt tgagcggcta gatgattatg acgtggtctt
    21661 ggttgcttat gaggaatcag caaagcaagg cgaaaaatca aatgcagcca aaattcttag
    21721 tgaaacaaaa ccagatagtc gaatcttagc tgtctttgga ccagaaggtg gattatcccg
    21781 cgatgaagtc gattattttg tagaacacaa agggatactt tgtggattag gaccaagaat
    21841 ccttcgtaca gaaacagcac ctctttattt attaagtgct gctagctatc aatgggaatt
    21901 aatgaatcct tctttcagct aacgttcttc attaataaaa gagtatcgac agaaagaatt
    21961 gaaaaaatag tcaacaattt gtataataga aacaattatt tatacaatgg atacgaaaaa
    22021 tcgtttgaaa aagcttttaa aaagcgaaca tttccgtaaa aaaacgtata actgcacgga
    22081 ttttgggaca attatcaaac agatatttgt cccgattccc gttttaccga aaaaagaaag
    22141 ggggatcgat tcctgtgaca aaagaagaaa ttttaacggg acccggtgtc ataaagctcg
    22201 ttagtcaata catgggtccc gaacatgtag catttgttga gaaagcatgc gaatacgcga
    22261 ctgctgcaca tgacggacag tttagaaaat caggcgaacc ctacattatt catcctatcc
    22321 aagtcgcagg tatattagca gatttaaaaa tggatcccca tacagtggct acaggcttct
    22381 tacatgatgt tgttgaagat acagaaatca ctttagaaga tctgagagaa gaatttggcg
    22441 atgacgttgc tatgttagtg gacggcgtaa ccaaattagg gaaaataaaa tataaatccc
    22501 acgaagagca gctagcagaa aaccaccgaa agatgctgct agcaatggct caagatttac
    22561 gagttatcat ggtcaaatta gctgatagac tacataacat gcgtacgttg aagcacctgc
    22621 gagaagataa gcaaagaagg atcgctcagg aaactttaga aatctatgcg cctcttgcac
    22681 atcgtctagg gatcagccgg atcaaatggg aattagaaga tacagcactt cgttatctaa
    22741 atccaaaaca gtattaccgt atcgtccact tgatgcagac gaagagagaa gaacgtgaaa
    22801 aatacgtaag tggtactgtt gaagatattc gaatagcgac agaagagctg gggatttttg
    22861 cagaaatcta tggacggcca aaacacattt attcaatcta tcgtaaaatg aaagatcaga
    22921 aaaagcagtt caacgaaatt tatgacttgc tagctattcg agtgatcgta gattcaatca
    22981 aggattgtta tgctgtatta ggagcaatcc acacaaaatg gaaaccaatg cccggcagat
    23041 ttaaggatta tattgccatg ccaaaagcta atatgtacca atctttgcat actaccgtga
    23101 tcggtccggc aggaaatccg gtagaaattc aaatccgaac acaggaaatg catgaaatcg
    23161 ctgaattcgg ggttgctgca cactgggcct ataaagaagg aaaaaatgaa aaagtagaac
    23221 cagatggtat gacgaaacaa ttaagctggt tccatgagat actcgaactt caagacgaaa
    23281 gctatgatgc ttctgaattt atggaaggcg taaaaggaga tatctttagt gataaagtct
    23341 acgtcttcac accaaaagga gacgttactg agttaccaaa aggatccgga ccattggact
    23401 ttgcatacag tatccataca gatatcggta acaaaaccac tggtgcaaaa gtaaatggca
    23461 aaatggtgca gcttgattac aaattgaaaa acggagatat cattgagatc atgacttctc
    23521 caaattcatt tggcccaagt cgcgactggt tgaaattagt tgctactagc aaagcaagaa
    23581 ataagatcaa acgtttcttc aaagcccaag atcgagaaga aaatgtgatc aaaggccacg
    23641 aatccgtggt caaatgtatt acagatctag gatttacgcc taaagatatt ttgacgaaga
    23701 acaaactgca agaagcactc gatcgtttta attatcaaac agaagatgat ctctatgcag
    23761 ctgtagggta tggagaagtt agccccttga cgatggccaa tcgtctgact gaaaaagaac
    23821 gtaaagaaca aaaaatcgag cagcaaaagc aagaagcaga agaaatcatg aatcagccga
    23881 aaaaagaacc tgacaaaatg aaagtacgtc atgaaggtgg cgttgtcatt caaggggtag
    23941 aaaacttact aattcgtatc agtcgctgct gtaatccgat tcctggtgat gatatcgttg
    24001 gttatatcac taaaggcaga gggatatcca ttcatcgtcg agattgtccg aatgttcagc
    24061 ctgacaaacc aaatgtagca gaacgtttga ttgaagtcga atgggaagat acatcgaata
    24121 cacgaaaaga gtatgatgca gatttggaaa tttacggcta taatcgttca ggcttattga
    24181 atgatgtact tcaaacagtc aatgcgctaa cgaaaaatct caacagcgtt gaagcacgga
    24241 cgaataaaga taaaatggcg acgatccatt tgacggttgg tatccagaat ttatcccacc
    24301 taaagagtat cgtggataaa atcaaagcag tacctgatgt ctacagtgta cgccggacga
    24361 atggatagga ggacagatat gagagcagtt gttcaacggg taagtaaagc tgctgtgtca
    24421 gtcgatcagc aagaaattgg aaaaattggc tacggcctac ttgttttatt aggcattcat
    24481 gaaaccgata cgcaaaaaga tgtggactat ctggtaaaaa aaattgcaca aatgcgtatt
    24541 tttgaagatg aacaaggaaa aatgaatatt agtgtagaag atgtgggagg acaaatttta
    24601 agtatctctc aattcacact gtttgcggat acgaaaaaag gaaatcgacc aagctttgtg
    24661 gaagccgcac gaccagaaac agctattcct ttatacgaat cattcaatga aggaatcaga
    24721 agtcgcggca tcacagtcgc taccggacaa ttcggagcag atatgtcggt ttctctcgtc
    24781 aatgatggac ctgtaacgat cattattgat tctaaaaaca aataaagtaa tctggttttc
    24841 actagtaaaa actatgaaac acgtcgctta agttagactg ataaactcta acttaagtgg
    24901 cttttttgtc tgcaaaaagc gcttcttttg tcagcagtcg gaaaaaaagt taagcttaaa
    24961 ttatacaagt tattttttat aggaggttgc tgaagatgaa agaagtacca gtagtagata
    25021 tcaaaatcac agatttaaaa aaattgtatc aaaaaggata caacatgttg gaggagttac
    25081 gccatgaagc ggacgctccc gtcgtcaaag caaagatttt caataaagaa gctatcacga
    25141 tctacggatc atcagcagca aaagtatttt atgatccaag gaatttcaaa cgaaaaggtg
    25201 ctatgccaaa attagtattg aagacacttt ttggtcaagg tggagtacag actttggatg
    25261 gagctgctca tcaccatcgg aaaaatattt ttatggatct aatgacaccg gaaagaatgg
    25321 aagattatca tcgtatctta gataagaatc tgacacaagc acttgaagca cagcatggtc
    25381 aatttgaact atttgattta agtaagatgg ttttctttac atctatttgc gagtgggcag
    25441 gtatcaattt atcagcgatt tctaaagatg aggtcgaaaa attagcagaa tatcaaatct
    25501 ctatgatcag cggaacgttt acttctccga ttgatcacat aaaaggtgtc gaaaatcgca
    25561 aaaaatcaga aaaatgggcg caaggcttga ttgaagaagc tcgacaaaat cctgtggctg
    25621 gaaaagaaaa tgtggcatta tatgcttttg ccaatgccac agatttagat ggtcagcttt
    25681 tacctctgga agtggcagct gttgaattat tgaacatcat ccgtccaacg gttgctctaa
    25741 ctgtgtgggc ggcattgatg ggacatgcac ttttcagtag accagatttg tatcagcaat
    25801 taaaaaatga cttttcgacg ttacaagatc cgtttattca agaaatgaga cgctattatc
    25861 ctttcttccc aatgctacct gcgatttcgc tgaaagaagt agaagtagat ggttatcgta
    25921 taccagaagg cagttgggtt attttagatt tatatggaac agaccatgat gaacgaacag
    25981 ttgaagcgcc agattccttt atgatcaaac ggtatgtagg gaaagcaaaa gatatttcct
    26041 ataaagaaga atatgagatg attgcacaag gcgggggaaa ttttcgacaa atgcatcgct
    26101 gtgcaggaga atggatcact cttcatagtc tacgtgtgtt ttccgatcag ctagtaaata
    26161 agtttgagtt ctctgtacct gaacaagatt ggacgattcc attcaatcag tttccgactt
    26221 atccgaatag ccgagcgcta ctttacaaga attagattag cataaactta tcgaaaaaaa
    26281 ggaagatcaa acagtgctga tcttcctttt ttcatattac tataaattga taaggtcaat
    26341 tgatcaaaat ccacgaaatg cttcatcaat ggtttgataa tcttcattgg ataaatgcaa
    26401 atctaaagct tggacattgt cagatacttg ctctggacgc ttagctccag gtataactac
    26461 agacactcgt ggattcttca tgtaccaagc taaaacaagt tgcgcaggag tggcttggta
    26521 tcttttagca atggggcgaa gtacatcgac agcggttaaa atttctctga aacgttctcc
    26581 ttggaaatct ggatttcttt ttcgaggatc tccttcacca aattgttttt cctcaccaag
    26641 ttcatatttt cctgtcagca aaccggaagc aagaggaaaa tagggaacaa aactgatttt
    26701 gtttttttca agataaggga ataattcttt ttcagcctgc cgatggatca aactgtattt
    26761 atcttctact acatcgacat atccatctgc attcgcttct ttcaattgtt ctaacgtgaa
    26821 gtttgacaca ccgactgctc ggatttttcc tgcttctttt aattcgtgca atgtagcaac
    26881 tgattcattt tttggtgtgc tttcatccgg aaagtggata taaaaaatgt cgatgtaatc
    26941 agtttgcaga cgttttagtg catcttccac cgattgtttg agaaactcag gagaattatc
    27001 aaatgttcgc cctttatttg gtacatgagc agcttttgta gcgatgacga cacgtgagcg
    27061 atcatattct ttttccttca gtacctcgcc aatcagttct tcggaacggc cgtttccgta
    27121 agcataagcg gtatcgatca aattgatccc gctattcaaa gctgtacgaa ctacttgttt
    27181 tcctgtctca tcttccaaac cgtcaaaaag attatgtcct ccaacggcat ttgctcctaa
    27241 tccaagctgt tctgcgtaga cttgtgaatg tccgattctt atttctgcca tagctgattc
    27301 ctcctttttg tctagtctaa aacaaaatac caaaaagctg aactaaaacg tttttgtgag
    27361 gggaaatagt cagatatctc ttgactttga gactggtttt tagtaggtta taagtaatat
    27421 cgaatgtcaa tgaaagagcg agtaagtttt tgaaacttta caagagagga tcatcgaggc
    27481 tgaaaatgat ctaaagtttc attagcctaa agacactctg gagatggacg agtgaaaagc
    27541 cgtcccgcgt acgagcgtta ttcgtaagag gaaagagaaa tctttcaaaa ttaggtggta
    27601 ccgtgtatga atttcattca cccttgttca tgatgaacaa gggtgatttt tttatttaca
    27661 tagctaagaa agggagagaa aactatgagt ttccaaagac caaaaggaac aaatgatatt
    27721 ttgccaggcg aatcagaaaa atggcagttt gtcgaagaaa cagcacgttt attattcaga
    27781 gattatcagt ataatgaaat gcgtacaccg atttttgaac actatgaagt gatatcccga
    27841 agtgtagggg atacaacgga tatcgtttca aaagagatgt atgattttta tgacaaaggg
    27901 gaacgccatg tgacgcttcg accagaaggt acagcaccaa tcgttcgttc ttttgttgag
    27961 cataaattgt tcggcccaga gtatgcgaaa ccgttcaaaa ctttttatat gggaccaatg
    28021 tttcgctacg aacgccctca aaaaggacgg ttacgtcaat tccatcaaat cggagtagaa
    28081 gcatttggat cagaaaaccc tgcaactgat gtggagagca tggtgatggc acttgatttc
    28141 ttcaaacagt tggggatcac gcaaatccgg ctagtcatca attcattagg tgataaagaa
    28201 acgcgtcaag cctatcgtca agcattgatc gactatcttt taccttttga atcagagctg
    28261 agtgaagact ctaaacgccg ccttcatgaa aatccattac gcgtattgga cagtaaagac
    28321 aaacgagatc aaaaatttgt ggcagaagct ccatctatct tagattatct aagcgagcct
    28381 gctcaaaaac attttgatac cgttgtttcc atgctggatg cattggctgt tccttatgaa
    28441 atcgacagta atatggttcg cggactagat tattacacac atacgatttt tgaaatcatg
    28501 agtgacgcac caaaaatggg ggcacaagca acgatttgcg caggcggacg ttatgacaat
    28561 ctagtggaag aactaggtgg tccttcaaca cctggcttcg gatttgcaat gggtatcgag
    28621 cgattactct tgactatgga ggcagaggaa gtagttatcc ctgcatttaa tgagttggat
    28681 gcttacgtgg tcgcactcgg agacgaaaca aatatcgaag cattgaaagt ggtccaagcg
    28741 attcgaaact ttggtttttc agctgaccgc gacttcatga accgtaaagc aaaagcgcaa
    28801 tttaagacag cagataaagc gaatgccaaa ttagtattgg tgatcggtga agatgagctt
    28861 gctagtcaaa cgattaatgt caaagcaatg gcaagccgaa aagaaaaaag ttttccatta
    28921 gaagagatat atgaacattt tgatgaagtc tatgatgaaa tgactttatt cgagtaggag
    28981 gaaaaaaata aatggcacaa agaacgatct attgcggatt agtttccgaa caacaattag
    29041 aacaaacagt tacattaaaa ggatgggtac aaaaaagacg agaccttggg ggagtaatct
    29101 ttatcgacct acgtgatcgt gaaggaatcg ttcaaatcgt ctttaatcct gaaatcaaca
    29161 aagaagcatg ggaaatcgcg gataaatgtc gcagtgaata cgtgatcgaa gtgactgggg
    29221 aagttagaag acgcggagaa aatgcagtaa atcctaaaat ggccactggt gaattcgaag
    29281 tgattgcaag tgaaatcgtg attttgaatc aagcaaaaac cccgccattt caaattgaag
    29341 atgatcaagc aatcagtgac gaaatacgtt tgaaatatcg ttatttggat ctgcgtcgtc
    29401 caaaaatgac taaaaacatc aaattaagaa atgcgacaac aaaagcgatt cgtcattatt
    29461 tagatgataa tgactttctc gatatcgaaa caccttacct agggaaatct acaccagaag
    29521 gcgcaagaga ctacctagtt ccttcacgtg tccatgctgg acatttctat gcgttgccgc
    29581 aatctcctca attgtttaaa caattgctga tgaatgctgg attcgaccgt tattatcaaa
    29641 tcgttcgttg tttccgcgat gaagatttac gtggagaccg tcagcctgaa tttacgcaag
    29701 tagatattga aacaacgttc ttaacagcag aagaaatcca aacttacaca gagggattga
    29761 ttgcgaaggt aatgaaagaa gttcgcggta tcgaagtcac attgccattt cctcgtatga
    29821 cctatgatga agcaatggct cgctacggca gcgacaaacc ggatacacgt tttgatatgg
    29881 aattgatcga tctaagcgat acagtcaaag aagtagaatt caaagtcttc caaatggctc
    29941 ttgaaaacgg cggtgtcgtg aaagcattga atgctaaagg cgctgcagat cgctactcac
    30001 gaaaagacat ggatcagtta ggtcaatatg taggacaatt cggagctaag gggcttgcat
    30061 ggctgaaagt agaagaagac ggattgaaag gtccaattgc taaattcatg ggagaagcaa
    30121 cagaagcaat cattaaagca acagatgcaa aaccaggtga cttgctgatg tttggcgcag
    30181 ataaaagtga aatcgtcgct gctgcattag gtgctatccg gacacgttta ggaaaagaat
    30241 taggactaat tgatgaatcg aaattcaatt tcttatgggt aactgattgg ccacaatttg
    30301 aattttcaga agaagaaggt cgatatgtct ctgctcacca tccattcacg atgccaaaag
    30361 aagaagatat cccgttattg gctgaagacc ctgcaaaagt ctatgcagaa gcttatgata
    30421 tcgtcttgaa cggttatgaa ctaggcggag gttctatccg tattcatact cgtgagcttc
    30481 aagaaaaagt actagaaact ctaggcttta caaaagaaca aatggaagaa caatttggtt
    30541 tcttgttaac agctttagat tacggattcc ctccacatgg cggtattgca ttaggattgg
    30601 accgttttgc aatgttatta gccggagaag ataatattcg tgaagtcatt gcctttccta
    30661 aaaacggaaa agcagcagat gtgatgagtg atgcaccaag tatggtttcg cctttacaat
    30721 tgttcgaatt gaatattgat gtaacgaata tagaagaata gccgataaaa aggtaaaaag
    30781 gatttgtcga atacgcttct tttgacaacc ttgatttaat tgaaaaacac tttataatga
    30841 agccataaca tataaagcgg ggcttttcca tgaaaaaaac attcgaaaat tgggcttatc
    30901 atgattacac gaccaagctt tcggtatcta tcgtttatgc gttgatcgca tcagtcgcat
    30961 tgaatttctt ttatcagcca ggacatattt attcaagtgg tattactggt ctagctcaga
    31021 ttttgacgac tttgtcaaaa tcactgatag gattcgaagt accggtatca acaactttgt
    31081 atttactgaa tatacctttg tttgtattgg cgtggttaaa aatcgggaag aagtttacaa
    31141 ttttcacttt tatcacagtc actttgacct ccctttgtat ccatatttta ccggaaacag
    31201 tcctatctac cgatccaatc atctgtgcgt tgtttggggg ggctgtgatg ggactaggta
    31261 ttgggctagc gttgagaaac ggcctatcat cgggcggtct agatatcgtg agtatcacga
    31321 ttcgtaaaaa aacaggaaag tcgattggtt ctatttcgat ctacttcaat gcattgatcg
    31381 tttttgttgc cggaattcta tttggctggc aatatatgtt ttacagtacg ttgtctattt
    31441 tcgtcagtgg aaaagtaact gatgtcgttt atactaaaca aaagaagatg caggtaatga
    31501 tcgtaacgaa acatccagaa aaagtagtaa atgaaatcca aaactgtatg cgtagaggga
    31561 tcacgatcat caatgaagca gaaggtgctt ataagcatga taagcagaca gtattgatga
    31621 cgatcgttac acgatacgaa ctgcctctac ttagagaagc aatgaaagct tctgatccac
    31681 aagcttttgt aagtattgca gaaaacgtcc agatacttgg tcgtttctat gaggatggca
    31741 tttagatcga gtttgacaaa aattcatgaa atactgctgt agacagacgt tcgtttgtct
    31801 gcagtttttt tctaccatag tagaatcagc aaagaaaaac tgctttttta tttgaaattt
    31861 tcttaaagtt gaaaaaaagc atgtttattt ttttaaattt atgggatgat agaaaaaaat
    31921 agtagagaaa ggacacgatt ctacttgcta ttttcactta atacccgtat attacgaatg
    31981 aaaaaagttt atgaaaagtt ggaatattga atggaaaaac aaaaaaggtt aaaaaacagc
    32041 cgctacatca ctgggtttga tgggctccgt tcattagctg tggtgggtgt tatcttctat
    32101 caccttctgc caactagtat gaaaggcggc tatttgggcg ttccgatttt tttcgtcgta
    32161 tctggttatt tgatcacgga tctgctgcgg caggaatggg aacaaaatgg aaaaatcaat
    32221 atttggcaat tttatgtcag aagaatgaag agactctatc cggcacttgt ttttatgctt
    32281 gtgacagctt cggcttatat cactttgttt cagcgagggc tgttaaacaa tcttcgagga
    32341 gttgtggtca gcagcttact gtatgtcaac aactggtggc aaatcaataa cggtttatct
    32401 tatttcgatc gatttgctaa tgagtcaccg tttacgcata tctggtcttt agcagtagaa
    32461 ggtcaaaact atttggtttg gccgatcttg tttgtattgt tgatggtatt tgttcgtaaa
    32521 agaaaatgga tcttttatac tgtgttaggt acatccttcc tctccgccat cttgatgatg
    32581 atcttgtata caccaggagg cgatccaacc cgtgtttatt acggtaccga cactcgtctg
    32641 ttttctatat ggttgggaag tgcgcttgcg tttgtttggc caagtacgcg tttaaagaaa
    32701 aacatcccga aacaagcgaa aaatattctg aatatcgctg gaggaatctc gcttttggca
    32761 ctgattcttt gcttcttctt tttagatgat cattacaatt tcatctatta cggaggaatg
    32821 ctcctagtga gtctgttatg cgtagtgcta gttgcagtga cagcacaccc cggagcaagt
    32881 ttgaatcgat ggctgacaaa tccagtattt acttggattg gcaaacgaag ctatggcatt
    32941 tatctgtatc agtttccagt tatgattttt tatgaagcaa aggtgactga tattgccgat
    33001 catgttttgt tgcatacttt agtggaaatc acattgattc taatcatcag tgaactttct
    33061 tatcgatatg tggagcgtcc gctagcacgt tttgattatg gtcgtacatt ccaagtgatg
    33121 aaaggatggt tcagcaaacc gatcatcagt aagagcaaac catgggttgt tccaggcaca
    33181 ttacttgtag caattgcact cgttggtttc gttacggcac cgaaaaacgc tgtaacagcc
    33241 gatcaaaaac aactgcaggc aaaaatcgct gaaagtaaaa aattagcaga agaaaccaga
    33301 aacaattcag aaaacaccac tagttcggtt gaccaagcga tccttgataa gtacgagttg
    33361 tccaaggcac aagggaataa ggcacaaaaa ttagaactaa cggcatttgg tgattcagtt
    33421 atgttggatg ctgcaacgga tctacaagaa ctttatccaa aagtagtggt agatggagat
    33481 gtcggacgac aattatatgc cagtgtaccg tacattgaaa aattaaagga acaagatttg
    33541 ttgaaagaca ctgttctgat cggactagga acgaatggct cattctcgga agctcagttt
    33601 gatagtgtga tggatgcaat cggtgatcga aaagtatact gggtcaatgt ccgagtaccg
    33661 acgaagcgct ggcaaaatga cgtgaatgcg atgcttgaac agatgacgaa aaaatacgac
    33721 aatatgacgc tgattgattg gtacagctac agtaatgacc atagtgactg gttttatgat
    33781 gatcaagtcc atccaaatcc tgaagggatg acacattaca ttcacttagt cagcgaagcc
    33841 ttgctaggag gaagttcaag tacgaacagc agtgagacaa acgtgacaga atgatagcta
    33901 caaaaaactt caggcaaaaa catttgtttt tgcctgaagt ttttttatat ttaccgtgtt
    33961 tttaacattc atatagattc aggaagcaaa acggttgagt gtgtctgtaa ataacgttct
    34021 ttttgttttc gggtgagttt tttgaatgca gcttctgctt ttgttgcttc gcttcttgaa
    34081 gaaaatactt ctgcatggat catcttaaca ggtcttcttt tttgaagacg agtatattta
    34141 gcaccagtac cattgttatg ttcctctaat cgtctagaaa gatcggttgt atatccacca
    34201 taaaatgtct gatctttaca gaataatacg taaaaataat gttcatttgc catagagcat
    34261 ctcacgaatc tctgcagtgt attctccatc catattgtaa gtgatcaatg gtggagcgac
    34321 tttgaatccg tctaatttgc cttctttgat tccttcaact aacaaaatat ttgcctcttt
    34381 tccaggtttt gggtaaacaa attggatcct tttaggtgca attcgatagt cttccatgac
    34441 ctgaatgatt tctaagaacc gatcaggacg atggaccata gtaaagcgtc catttgtttt
    34501 tagtagctta cttgaaatat cgatcacttc ttctaacgtc gtatgaatct catgacgggc
    34561 gattgccagg tgtttattag ggttcgtttt atttgttggc agccctttga aataaggtgg
    34621 attacagacg actaagtcac aagaattatg tcgaacgaca gataagctgt ctttcagatc
    34681 gatggtgtgg acagtcattt gttcatttaa atggttaagc gcgatactac gtcttgccat
    34741 gtctgctaga cgaggttgaa gttcgatagc gtcgatagcc gcctgggttc gtttacttaa
    34801 aaaaagtcca actgcgccat ttcctgcaca aaggtcaaca atcttccctt ttttaggtac
    34861 acgggaaaag tgagcaagca gcacagcatc taaagaaaaa gaaaacactt tggaactttg
    34921 gataatcttg atatcttctg cgtataattg gtcgattcgt tcgtcttcgt gaagcatgga
    34981 ttccctccaa aatgaaaaaa atagtcgtct gtattatact atttttatta aaaagaacca
    35041 aggtggcttg tcaaacctct attttttatg catactatga ccataagaag ggaaaggaag
    35101 agactatgtt ttatcgattt atgcgcgggg tagttcgagt actattagcc ttagttaatg
    35161 gaaatgcccg ctatgagaat aaagaagttt taccgcaaga tgagaattac atactagttg
    35221 ctccccatcg tacttggtgg gacccattat atctggcggt agctgcagca cccaaggaat
    35281 tcagctttat ggcaaaagaa gaactgttca aaaatccagt tctgcgattt atattaactc
    35341 gatccaatgc ttttccagtt aaaagagaaa atccaggacc aagcgtcatt aaaacacctg
    35401 taaagatttt gaaatcgaca aatttgagtt tgatcatgtt tcccagtggg acaagacatg
    35461 ctacagaact taaaggcgga atggcattga tttccagaat gtctaaagcg aaaatcgtgc
    35521 cagctgtata tcaaggtccg ttgacacttg gggatctgtt caagcgtcag agagtgacag
    35581 tgcgtttcgg cgaaccaatc gacttatcag atatcaagaa aacggataaa gaaggtatgg
    35641 cggagatcga acgacgaaca caagcagctt ttgacaaatt ggacaaagaa gtgaaccctg
    35701 atttcaaata tgaagtcaaa aaaaaataaa atgataagtt tatgcgtggg cggtcttttg
    35761 aatctactgt cctgttcagt tcagactttt tcaatgaaaa cgagggagag acgtcaagtc
    35821 tgccctcttt ttgcgttcca atatgtaaaa tagaagacag gtgtcatgta atggatcaaa
    35881 gtgttataat aattagaagg acattttttg aggtgattga atgcgttttt tacatacagc
    35941 cgattggcat atcggaaaaa aactacaagg ttatgacttg attgaagacc agcgtcatgt
    36001 actatcttct atactagata tcgcgaaaga agaaaaagta gaggcaatcg tgattgccgg
    36061 agacttatat gataggagtg tcccagctgt tgatgccgta gaattataca atgaattgat
    36121 ggtcgattgg aatttaaagg aaaagatccc gatttttgct atatcaggaa atcacgatag
    36181 cagtacgaga ctttcagcgg gtacgccatg gttcagttat tcgaactatt ttttgcgaac
    36241 acagctagca gaagcatttg aaccagtcga gtttaaaaac gtacaatttt tctttcttcc
    36301 ttactttgaa ccaattgcag cgagattata ttttgaagat gacaatatcc gaacgatcaa
    36361 gcaagcggta gccaaggtga tagataaaat gcaggagcta tttgattcag aaaaaaaaca
    36421 agtattgatt agccattttt ttgtaatggg aagtgtaaaa tctgattcgg aaacgaaaat
    36481 cgaagtaggc gggttggatt cgatccctgg tgaactactt acaccgtttg attatgtagc
    36541 actaggacat ttgcataatc aagctgcttt gagacaggat aatgctcgct atagcggttc
    36601 cccattgaaa ttctctttat ctgagatcaa ccaaaaaaaa ggcgtatgga tcgttgacct
    36661 gacaacaggc ctggagttga attttaggga gatcgaaccc ctgcatgata ttgaagaaat
    36721 aacagcgagt ttcaaagaat tgctggatcc tgccttttat cagtccgtca atcgggagaa
    36781 ttacatctat atctatctca ctgatagaac gatcatcccg aatatgatga accagcttcg
    36841 gcaaatatat cctcgtattt tggggattga acgggcaaac ggtcgagcag aacaaacaaa
    36901 gcgcaaacga aaagtactag aaatgaaaga tcctcaaaaa ttagcagaag cattttttag
    36961 tgaaatgacg gaagagcagc taactgcaaa ccaagaaaaa tggctcggag aaacgttgca
    37021 agaaatcatg gaaatggaat gatcaagaat gaaaccgaaa aaattacaac ttaaaaattt
    37081 tggtcctttt atcaatgaga ccgtagattt ttcacgtttg acagaagcac cgcttttttt
    37141 gatcagcggg aagacaggag ctggcaaaac gacgattttt gatgggatca ccttcgcact
    37201 ttttggtgaa acttccggaa gacttcgctc tggaaaagag atgcgttctt tgtttgctac
    37261 accagacgaa gaaacaagcg tgactttttc ttttgaacat cagcagatga gctatgaaat
    37321 cgaacgaaaa ccagaacaag ttttagcaaa acgaaaagga aacggaacaa aaaaacaggc
    37381 agccaaagta acgttgacca tttttgatgg gaaaggaaaa gaaataaaac aaatcacaaa
    37441 acgtacagaa gcagatcagt tgatcagaga actgatgaat ctcgatgcga aacagttttc
    37501 tcaaatcgtt ttactacccc aaggagaatt ccgtaatttt ttggtttcat caagcagtga
    37561 taaagagact gttttacgtc atttattcgg tactcaattt ttccaacagt tcaatgagcg
    37621 attaaaagaa aaagcaaaac aacagcaaca aaaattggat catttagagc aagagctgca
    37681 tcttctgcaa aaaaggtttg ttttagtcaa agaagaggaa ccagcagcag ggtttgaagc
    37741 cgttttagcc caatggacaa accatcaggc aatactcgaa aaacaaatca cggaagaaaa
    37801 agaagctcta acggaacttc aggaaaaaca aaagcaacta gaaaattcct actatcgttt
    37861 tgaaagcttg aaaaaaagtt atgaagaaaa aacgatatta ataaacaagc aaaaggaatt
    37921 agctgctcaa aaagacgaaa tagaggaaaa gaaacgatgg attgggtatt atgaatttac
    37981 gcaacgatta gtcgaaccaa tccagcgcct acaagaaata aaagttgaaa aaatagaact
    38041 gtcacaaaaa agtagtgagc aaacaaagaa actagcgatg actacgcaga attatgaaga
    38101 gtggcaaaaa aatgaagcaa accgcgtacg ccgacaaaca aaaatcaaag aagctgctca
    38161 aaaactgcaa aacaaacaga tgatggtgcc aattgtcgaa tccttacgta gtaaaaaaca
    38221 agacgcagaa caattaacaa tttcattgga taaagaagaa ttgaatgccg agaagctgta
    38281 tcaaaatctc gctatctatc aggaaagaac tcgaattctt caagaaaatt tactggatca
    38341 ggaaaaactg caagaagatc gtcttgcttt tggacagtta aaatatctag atgaaaagca
    38401 gcaagaaata gaaaaacaaa aagcaaaaga cactgggtcg ctcgcacaga taagagaaga
    38461 actcaaaaaa gcaactgaag aaataacaaa aaatagtcag ctattgaaag aacagacaga
    38521 acaagcagaa cagctgaaaa gccaatgggc aaagcagcaa attgctcgtc ttcagctttt
    38581 tttgcgtcca ggggagccat gtcttgtttg tggatcaacg gaacacccca tacaacaatt
    38641 aaaacacgaa cagccaaata aggaagatat cttaaaagta gaacaacaac tggcagaagc
    38701 tgagtctgct gtagaaagaa ctcagcaaat cattgccaaa agtgaagcac atgaaaataa
    38761 tcttcaacaa caattatcag atttagaaca aaaaatcatg gcaacgaata tacaattggc
    38821 tgaacaagaa gaacacttgc aacaggcttt gaaaaaggca tttcctgaga taacagaaca
    38881 aaaaacaaaa acaactcttg tagtaatcga acaatcttta gatagacgaa aaagcaagat
    38941 ggccaatgca agaatagagc tggaagaaat aagacaacaa gcagaaaagc tcgaacaaca
    39001 attagctgtc tacaaacagc agcagacaga aaaaaataat cagctccagc aactgcaagg
    39061 agaaatcaaa agtttagaac aacaattgca agatacggat agtaactgtt tagaagagga
    39121 gatacgacga cttgaaaaac agttagaaga agactcgaat caactagctg ttgagcagaa
    39181 agcaggagtg caattgttgc aagatcttca tacgcttcgg acacagcttg atcttcaaca
    39241 tgaacaaata gaaaaaatag atgaaaaaat agaaaaggaa caagaaagaa tagctaaaaa
    39301 gttagaaagc cagtcgtatt tttcaacaat tgaagaaatc tgtgcatttg cacaacagca
    39361 agcacaatat aaagaaaacc aacgtacagt tacttcttat catgaagagc aattagtgac
    39421 agctgaccgg ctgcgtcaat tagaaaatat ccagctaact gagacattcc cagatacaga
    39481 agagatcagc caacagttag aagaaataag acgtatgatc gaaataaagc aagaaaagat
    39541 atacggttta caagaacaaa aaattagtaa tcaaaaaatc tacgatgaat gtctttcgat
    39601 ttatcacaca agccagacga aattagatga gcttacgcaa ttacagcagc tttcgcaaac
    39661 attcaacgga gaaaatccta agaagactag tttggaacgg tatgtgcttc aggtttatct
    39721 tcaagaagtg ttacaagtag caaatgatca tctccaacgt ttaacgaaaa atcgttatca
    39781 atttgagcta gcggatacca ttggcagtta tcgagggaaa accggattag aaatcaatat
    39841 ctatgatgat caggcaggta tgtctcgaag tgcgcatacc ctgtctggcg gtgagagttt
    39901 tattgcagca ctttctttag cattagctct tgccgatgtg atccagacac aagctggtgg
    39961 tgtagcaatc gatgcattgt ttattgatga aggatttggg tcattagatg aagaagcttt
    40021 ggaaatggca atggaagctt tagaaacgat cgaaagtgaa ggaagaatga tcggcatcat
    40081 tagtcatgta agagaattga aagaacgagt attacaacaa ataagaattg atacagaagg
    40141 tagcggacaa agcaaaatac gctatcaact tggataaaaa gggagattgg taactatgaa
    40201 atggagtatt ccagaaaaag tcattgcacg tggacgtcaa tacttgcaag aaggccgagt
    40261 gttatcggtt gtccctgacc aagaagaaaa cgtgtggc
//
LOCUS       DACEXQ010000004        40011 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.004, whole genome shotgun sequence.
ACCESSION   DACEXQ010000004 DACEXQ010000000
VERSION     DACEXQ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.004"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..811)
                     /locus_tag="IXH04_000145"
     CDS             complement(<1..811)
                     /locus_tag="IXH04_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1850785.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLG"
     gene            complement(885..2582)
                     /locus_tag="IXH04_000146"
     CDS             complement(885..2582)
                     /locus_tag="IXH04_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ1850786.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(2575..3888)
                     /locus_tag="IXH04_000147"
     CDS             complement(2575..3888)
                     /locus_tag="IXH04_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ1850787.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(3888..7406)
                     /locus_tag="IXH04_000148"
     CDS             complement(3888..7406)
                     /locus_tag="IXH04_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAQ1850788.1"
                     /translation="MDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEK
                     KEISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMV
                     NFYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETL
                     YKDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDV
                     LFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGS
                     SNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAI
                     GRNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVS
                     PFGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYK
                     FSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSS
                     SIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFET
                     KMPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEY
                     TSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNI
                     NKLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDG
                     KYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRL
                     YDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTL
                     DGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQP
                     LHLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIID
                     GVLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPND
                     SSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTL
                     KVEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYIL
                     LAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(7699..8157)
                     /locus_tag="IXH04_000149"
     CDS             complement(7699..8157)
                     /locus_tag="IXH04_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1850789.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(8455..8973)
                     /locus_tag="IXH04_000150"
     CDS             complement(8455..8973)
                     /locus_tag="IXH04_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAQ1850790.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(8975..9592)
                     /locus_tag="IXH04_000151"
     CDS             complement(8975..9592)
                     /locus_tag="IXH04_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAQ1850791.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSFYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(9589..10443)
                     /locus_tag="IXH04_000152"
     CDS             complement(9589..10443)
                     /locus_tag="IXH04_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1850792.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(10440..11351)
                     /locus_tag="IXH04_000153"
     CDS             complement(10440..11351)
                     /locus_tag="IXH04_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1850793.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(11392..12726)
                     /locus_tag="IXH04_000154"
     CDS             complement(11392..12726)
                     /locus_tag="IXH04_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1850794.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            12934..13764
                     /locus_tag="IXH04_000155"
     CDS             12934..13764
                     /locus_tag="IXH04_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAQ1850795.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(14008..14257)
                     /locus_tag="IXH04_000156"
                     /pseudo
     CDS             complement(14008..14257)
                     /locus_tag="IXH04_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14448..14642)
                     /locus_tag="IXH04_000157"
     CDS             complement(14448..14642)
                     /locus_tag="IXH04_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1850796.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(15147..15332)
                     /locus_tag="IXH04_000158"
     CDS             complement(15147..15332)
                     /locus_tag="IXH04_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1850797.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(16182..16523)
                     /locus_tag="IXH04_000159"
     CDS             complement(16182..16523)
                     /locus_tag="IXH04_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ1850798.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(16523..16753)
                     /locus_tag="IXH04_000160"
     CDS             complement(16523..16753)
                     /locus_tag="IXH04_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1850799.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            17040..17378
                     /locus_tag="IXH04_000161"
     CDS             17040..17378
                     /locus_tag="IXH04_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1850800.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(17583..17822)
                     /locus_tag="IXH04_000162"
     CDS             complement(17583..17822)
                     /locus_tag="IXH04_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HAQ1850801.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(17798..18145)
                     /locus_tag="IXH04_000163"
     CDS             complement(17798..18145)
                     /locus_tag="IXH04_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAQ1850802.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(18877..19458)
                     /locus_tag="IXH04_000164"
     CDS             complement(18877..19458)
                     /locus_tag="IXH04_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1850803.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLVKERLLTSQKVL"
     gene            complement(19641..20267)
                     /locus_tag="IXH04_000165"
     CDS             complement(19641..20267)
                     /locus_tag="IXH04_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1850804.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(20289..21620)
                     /locus_tag="IXH04_000166"
     CDS             complement(20289..21620)
                     /locus_tag="IXH04_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAQ1850805.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(21775..22176)
                     /locus_tag="IXH04_000167"
     CDS             complement(21775..22176)
                     /locus_tag="IXH04_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ1850806.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(22262..22468)
                     /locus_tag="IXH04_000168"
     CDS             complement(22262..22468)
                     /locus_tag="IXH04_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1850807.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(22563..24065)
                     /locus_tag="IXH04_000169"
     CDS             complement(22563..24065)
                     /locus_tag="IXH04_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAQ1850808.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(24083..24538)
                     /gene="argR"
                     /locus_tag="IXH04_000170"
     CDS             complement(24083..24538)
                     /gene="argR"
                     /locus_tag="IXH04_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ1850809.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(24636..25253)
                     /locus_tag="IXH04_000171"
     CDS             complement(24636..25253)
                     /locus_tag="IXH04_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1850810.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            25474..26847
                     /locus_tag="IXH04_000172"
     CDS             25474..26847
                     /locus_tag="IXH04_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ1850811.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            26922..28034
                     /locus_tag="IXH04_000173"
     CDS             26922..28034
                     /locus_tag="IXH04_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAQ1850812.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(28106..29743)
                     /locus_tag="IXH04_000174"
     CDS             complement(28106..29743)
                     /locus_tag="IXH04_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAQ1850813.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAVVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(29911..30519)
                     /locus_tag="IXH04_000175"
     CDS             complement(29911..30519)
                     /locus_tag="IXH04_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1850814.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(30904..32055)
                     /gene="queG"
                     /locus_tag="IXH04_000176"
     CDS             complement(30904..32055)
                     /gene="queG"
                     /locus_tag="IXH04_000176"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAQ1850815.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(32074..32826)
                     /locus_tag="IXH04_000177"
     CDS             complement(32074..32826)
                     /locus_tag="IXH04_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1850816.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKNADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(32967..33917)
                     /locus_tag="IXH04_000178"
     CDS             complement(32967..33917)
                     /locus_tag="IXH04_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAQ1850817.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(33918..34757)
                     /locus_tag="IXH04_000179"
     CDS             complement(33918..34757)
                     /locus_tag="IXH04_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ1850818.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(34754..35500)
                     /locus_tag="IXH04_000180"
     CDS             complement(34754..35500)
                     /locus_tag="IXH04_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ1850819.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            35738..37108
                     /locus_tag="IXH04_000181"
     CDS             35738..37108
                     /locus_tag="IXH04_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1850820.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(37163..38059)
                     /locus_tag="IXH04_000182"
     CDS             complement(37163..38059)
                     /locus_tag="IXH04_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1850821.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(38340..39017)
                     /locus_tag="IXH04_000183"
     CDS             complement(38340..39017)
                     /locus_tag="IXH04_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ1850822.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(39108..39929)
                     /locus_tag="IXH04_000184"
     CDS             complement(39108..39929)
                     /locus_tag="IXH04_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1850823.1"
                     /translation="MPFHSFGPAIRNDYILHVVMEGKGSYHVKNQQFQLKKGDLFLIR
                     PGDSTFYLSDGEDPWMYCWLSFGGSAAEEIIRQSLFREDQYTMVSSDIQKYIDIILEC
                     MKDTEETLENELQLTALTYRFLSVLLKDGGKVSGGVQKVHSSLAVETVKYIEAHYAEK
                     LSVEEIAQHLAVNRSHLSRVFKNHMGTSIKEYLIGVRINRGAFLLSLTDESVESIAYQ
                     VGFNSLVVFSRMFKKSTGETATNYRKRMKNKNFKNIALDTLKKQLEKQTTVSWTT"
BASE COUNT        11187 a         8403 c         6940 g        13481 t
ORIGIN      
        1 tacctagaag gagtataagt aatcgataaa gatggtattt ttttgtctgt tctatctttg
       61 aagagacttc ttctgtaaag ggaactcgtc ttcctgtcac tagtaatcgg tgagtgttca
      121 cggtgtaagg tgtacaagtg ataagtgtga ctagatcctg cttttctcta attgctaatg
      181 tatttgtttt tgttggaagg acaacaattt tctcaacgac ttcataagcg agcatccgac
      241 catacacttc aagatagaat cggtcatctt gctccatttc ttcaagatcc gtaaagagtt
      301 ttttctcagc aataccgcta tgggcggtaa tcacggaatg tgtactctct cctccagttg
      361 gaaaagaggt gccttgcagg actgtcgctc ctctatctaa caaaagatca ttcgtttcat
      421 caaataaagg caagctaaca ttgattttcg gaatatagat ggctccgatc atatgttgtt
      481 cgtaatattc ttttcctgga ttttggacat ttctaaccgc ttgctcaaaa ggatctttga
      541 cttgtcccat tcctgggatt actgatgtat gagcaagcgt ctgatttttc ttttcatgca
      601 ctgctaattg ttgctcatgt ttttcttctt tatcgcgttc aagctgcttt tgataattct
      661 cgatccttct ttgatccaaa taattgctga gagcatctgc taaaaatgga tatgtaaaaa
      721 ctgcagctcc ggcaagaaac atcactgcca taaataattg taataaaaga ctgatttggg
      781 tttgaggtct attacctatt ttctttttca ttttcttcac cttcttattt aaaaaaactg
      841 cagcaacctt tataaaaccg attgctgcag cttatttttg ttgcttatac ttcagacttc
      901 attttagatt tacggtacca gctgtacgct ccaatcatca aacttattcc gattagtaag
      961 aatgccaaga gcccgttccc accagtagca ggaagtgacc cgcgtttcat attcggtact
     1021 ctttctggcc ctggattttc tactcctacg atctgaattt cttcattata gcttccataa
     1081 gagatgataa attcagtaaa ggctgcctca cctgttggaa gtacataacc ttctggcgcc
     1141 atggtctctt tcatttgata ggttccataa tctaaccctt gaacctcaaa gtatccattt
     1201 ccatctgcga caaattctgt tgcttgtccg cttgttgccc actcaattct atctgctcct
     1261 gattcataaa cagctaaagg cgactcccct ttataaaata ttgcgtattc agaacggtct
     1321 tcattccaca attcgaatct tgcatcttta agcaattcac tgcttgatgc atcatatttt
     1381 tcaaactttc ttccacctgt tgtgactttc tctggcggct ctactccttc ttcataatca
     1441 aattcatcat ttcgtccgat ttcaataacc gctgtgttgt tatggaaatc atccgcttct
     1501 agttctgtgg aaaccttcaa attatacgtg atcgttaaat ctcctccacg tgcatctgct
     1561 aatctaccta aagtatctac tttggctact ccattttctt ctgtcaaaat cgtcacgaca
     1621 aacccgttgc ttactacttc tactatataa tcttgtcctt ttgttaaaat cgtctcgccg
     1681 gctcgcactt ctatttcctg atcataataa gttagaccaa ctgtaggcgt atctgtgatt
     1741 ttgtaatttt tgataacagc gcttccgttt tcaaggacag aatcgatgaa ataaggaata
     1801 ggtgctgtga tcgtgtaaga agccgttttt cctctttcta gatcaatata agaaatcgtt
     1861 tcttgttctc cagcttcatt aataatcgtt acttgtctaa ggtcgctttc ctctacattc
     1921 atctgtttat ctgcttctct gatttcgttt ttcggataca aatgcacatc tgtattgtat
     1981 gtgtctttcc atgtagaacc atcccacacg ccatccacca tatcaggcat catcactggc
     2041 atcataacaa ccattggaga cgcgattctg gaaatatgag ctggcgaata agtttctaag
     2101 aaaagataga ctttatctct agaagaaggt gcttcttccg tcacttggac ggtagagaat
     2161 accgcttctc cagcttcatt cgttgtttgt ttgtcaatac ttgtcagtcg atattttaaa
     2221 aaccaacttt ctgtgtgccg tttttgaatc cattcaatcg cttcggcaga attaagaccg
     2281 tcttctcgtt ccggtgttaa aggattatct cgaatcaaat cgtaatatac ttccgttacg
     2341 tcaaacaaat caaaatcgac tcctggaata ttttcttcac caaaatccgt agaaaccaat
     2401 ccgctattcg gtcgttcttc cggaacagat gagaaacctt ttttgtgtaa tgtaatcgtc
     2461 acttcacttg gtttttgtgc gacttcttcg gctttgacta gttttccgtt cccgaaaatc
     2521 cctattcccg cagtaacgag taacagtaca ctcattcccg tgacaatcat ttttttattc
     2581 atttttttct ccccgttctt tttttattaa taaaacagca cctaacaata aaagaattcc
     2641 tagtaaagaa atcaacgttc ttgcttcatt cgtttgcggg aatacggacc attttcctga
     2701 ttgacctgga ttgcttggac cactcggctc tttcggtttg tccgttgttt cttttacggt
     2761 ctcgttataa acacgtggtg ttgcggattc ataggcagag actggaactt tcccggattc
     2821 taattcagcc atctcctgtc cgttaactac tacatattgt ggttggcctt ttgcatccac
     2881 ccaaaattca gggatgatga cctcgattct cttacttgct tcatccatct cgtaaccttc
     2941 cacacctttt aattcttcaa aataataggt gccagaaggc aagaaacgtt gtcctgtcgt
     3001 aacaaggccc tctttatccg aaatgaattt gcttacattt ttgtcattca aaggatcatt
     3061 gtctgctggt ttgatccatt gatttttcaa gtcattagca ggagccatat ctaaataata
     3121 tttcccccct tctatcatct gatatagagc aaaaacagca ccttctaatg gttttccttt
     3181 ttcagttgtt cctctttgac gtccatactt gaagaagtat ggatctctta gataaccgac
     3241 atttttagga tagatatgaa tctcttgcag ttcctcggtt gattctgtcg ggtgataaat
     3301 tggcaaaata gcagcaattg ggatagctgt ctgatcgata tcaacatcca atccaacttc
     3361 tggatcaatc gattcttcga aaatcaaata aactgcatcc cgatcttggt ttttcttagg
     3421 tgctttgata cgtgcaatcc cgtctcctaa tccgccaacc gtatctttcc cagttgttac
     3481 cgaagtccct atttggacaa gcctttcttc tgtgattagc tgacggatat tgtttctgtt
     3541 cgtttcagcg atttgctgga cgaattcttt aggagtcatg ttttcagcag cttttttttc
     3601 atgataataa tcggtcatgt cgtaaatggc aaatgtcgct ccatttagtg gcaccgtctg
     3661 tgaaatgata tctgctgttt cccctgcttc ctcagagata gccaagccat cattttcatg
     3721 gtattcaaat tgatcgttat aatcgatatc tcgaatcatc cgtttatgca acacgaattc
     3781 cacgacatct tcccctgatt cttctgccga catcgcatgt ggaagaagga ctgctgcaag
     3841 cggtagtaaa acaagtaatt gaagcatcaa acacaacagc tttttcatta ccttgctccc
     3901 tcctttttag attgtctgac aaacaaacca taggctgctc ctgcaagcgc catgaagaat
     3961 gcagcaagca ggatatagaa taatcgtccg gatcctcctg tagcaggcaa cggattttca
     4021 ggatcgttcc cgacactaag ctgtaatctg attcgatttg tctccaaaag ttcatattct
     4081 aactcttgct gtagatcagg cgtctctgct tcttcgtttg gataatacga aatctcaact
     4141 tttagtgtac ccgcttcttt tcctggaact acccgatcat tttctatgtc aggttcttct
     4201 cgataactaa tttctaaaat aaagtaacct ggaagttttc tatagctgtc aggtgacttc
     4261 acttccttga tccgatagac cccttccaac aagtctgaag cattgaatag ggaagaatcg
     4321 tttggatctg gtgccaagcc tccctcgatg atttcatact ctcctgcact attttgtttc
     4381 tcaattgaaa attctgcgcc ttcaagacgt cggctattca tactgtcaat tttcaaaagt
     4441 tccaaatcga ttggcgctag ttcatcttct aaggttactt gtagtacacc atcgatgatt
     4501 tcatagcctt ccggcaaggg agcagtttcg tcttcgatga ccgtatcttc aatgataaac
     4561 ttaccttctt cattcagtgc aaactggaac acttgatcag atagccgata cccatttggt
     4621 gcgacagatt ccgagacctc atattcacca atagacagat gcagaggttg gtcgtaattt
     4681 aaatcgacat acttttctac tcctacggca cgagaataga agtatccgat cccaggcaaa
     4741 cgtttcgatt cttctggttc taacggatta ccagtgtatg ctcgatacag cgggaaggaa
     4801 gaatccccca ctttcctgat atcgaaacct gcaccatcta atgtatggcc ttcatggtct
     4861 aattttctaa ctcggatctc ccgataatca aacgtattgt aagcagtgaa tgctacccat
     4921 aaacgatcct cttcatctgt tgtatacgtg agatattgcc aattttcttc acctgtttgc
     4981 ttgagttgga tagtccctat agcattatcg taaagtcgca agatccatgt gtcattttca
     5041 tcaggttctt tgaaaccttc tgggttctgg ctctcaatga ttttgtattc tgcatagttg
     5101 gtagtttctt cactatcctt ataaacaaat gaatctggct ctccgattgg atgaataact
     5161 ttctcaccat ccacgattac atatttcccg tcttcatcta ctgaatagaa cgtgagtctc
     5221 cctttctctt cctctacttc gttcacacca gaaagccctt tcgctactgg atatccgatt
     5281 tccagtccgt taccatctgt ttcataaagt tcaaactctg cgccattgat tggcatatta
     5341 ctgccttcaa ttagtttatt gatattcatg tggattggct tgaattcatt tcgcatttcc
     5401 agtttgacat acaactcatc atactctgtg ccttctatgt taggctcttg tacaacagaa
     5461 agcttgtgat attctccttc ataataggaa ttcggatctt taccttccgg tccgccgact
     5521 ctcgaaccag atgtatattc atctacaagc tcgtagtgac ctaacatctc atcttcgttg
     5581 gctgcatatt ttttgatttg gaattgatac gtctcatcta ctaactgata acgtggtggt
     5641 ccagcgatct ctctgatcca aaatatgtcg ctgactgctc cagtttcatc tcctaaaatc
     5701 ggcatcttcg tttcaaagat cgctattccg tccgtggtag ttttacttgt ttcgattggt
     5761 tcggcttccc cactctctgg attttggtca tataatgcaa attcgactcc agccaacggc
     5821 tcgccatttt gacccttctt gctgacttgg aaattacggc ctttatgttc gtaacgaatg
     5881 gaagaagatg ggaaatacat ccgtccagta gtgatatcat tctcataatc ctccaggttc
     5941 tgaatatagg ccatattgtt cactgtgcaa aaatttccgt cttgagctcc cgcatcaagt
     6001 tttacttggt acttgatcct gaccatttcc ccttcaccta aagaaatcgg agaaaattta
     6061 taagcttggt tttcccgtcc gacatacgtt gtcggagtcg ccactacttc tggtggggct
     6121 tgtccatgac gttcttcatc ccatatttct ggctcttggt acttcatgcc gttttctcct
     6181 ggatctagat ggtaaaactg aatatctgcc gatctgccag ctccaaatgg tgagacatta
     6241 aaaccagttt catcataaag tgtggcttgc tggatcgttc tcttgaaggt agaagagata
     6301 tcctccaagg ctgtaacaat gtcattcttg ttgttcgcat tgtaatatcg cggtgttacg
     6361 ccatcttctt ctaaatcagc aatatttttc aatacatttc gtccaatagc atctagtcgt
     6421 tgtctcccgc taacgtttcc atcgattcct aaaccaatgg agaagatact tggcgggttc
     6481 tcttgatact tgtttttcag ccaatgatga taagcaactg tgttgatcgc agatgacttt
     6541 tcttgcgaag taggactgcc attcgtattg ttattagaag aaccatcacc aacccagtga
     6601 gtcgctttat tatctccttc ttttacctct ccataacgat agtgtacatt tacttcatct
     6661 tctggacggt taaagtcaga tgcataggta actggatgag taaaccctgc attagaagta
     6721 ttcccagaaa actcctcaaa atttctgaac aaaacgtcat tatcttcagc aatcttatct
     6781 tccatcacag tccagttgct ccaagctcca ttagtgctcg tacgataact tgactgaatc
     6841 ggtgcataac tgaaggtcgg tgtcccatct cccacaacga ttaggatttt ttctggattt
     6901 ctattttctc cgccattgtc tttatacaat gtttcatagc catttttaag ccctagtgta
     6961 agcggagttc ctccgattgg tgtacgattt aatgctgtat tctcacttcc cgtcaaacga
     7021 ttgatatcat ttgtcaatgg aaagatgtcc gaactgattt gctcatggtt attgatagct
     7081 gtactattat ggtaaaaatt caccataccg attcgaatat ccagactcgg gtcagagagc
     7141 agatctttac tcatttcgtt tactgcctca atcaacgcag catttttagt ttgactattg
     7201 gtcccagcgg ttaattcact catactcgct gacttatcta gaacaaaaac gatatcgatt
     7261 ggactgattt cctttttctc gcccccgatg acatctagat agacattgta aagggttgtt
     7321 gcatcttcag gatgtagatc agatgctgtg ctgcttggca gaacaggttc tacccatttt
     7381 ttgatgtacg ccccgttata atccaagtaa ctgttttcaa aattccgttc atttccccaa
     7441 agctgaacgg tttgagctgg aacgtttcta tcttcttcgc ctgcagttga tccattatta
     7501 gtagcatagt cgtaatttcg ccagttcgct cccccgtttg tataatcact atcatcgatc
     7561 aagttctctc cgtcatatgg cgagcctttg tctccttgat agcgttcatt gctttgtgtc
     7621 caatttccta acacttttgt atagtcattc gttgtaaact tcatctattc tttcatcaac
     7681 caccacttga agaggtagtt acccttcgtt cacatcgggg atcttttctg tttcaaaaat
     7741 caatgtgccg gtttctgctt cttttgaata aacttttccg acataccatt cttgaccatc
     7801 gatcgtctcg acttttaggt cttctccatc ttcatcatag gaggatagac ctgttccacg
     7861 aacgtttttt acggtaccaa tcccggcggc tgccgcatcg attgctaaac gtattctacc
     7921 ttcattggca ctttcctttt tttcaaaagc cagatgccat tcaagggttt cttctttttc
     7981 aatatacttc ccttcaattt ttgcatagtc ctcatctaag aatacctcat cctcctttgt
     8041 ttctgttgac tcagcagcta catccagggt cgtcatatga tagactgact ggaaatatgg
     8101 tccaaatagc aaactgacag ttactaactg caaaagcttc aattttaacc ctttcacatc
     8161 cttatactcc tttcaaaaat atttgtcttc tttcacaaca atcaacaaaa caatgcccat
     8221 ctaattataa tttaatttaa ttttaatttt aaatcaaatg atatcctttt tagttaaaaa
     8281 acaccaatta tacaacattt attattaaaa ttaaattata aaattttata acgttctcat
     8341 ttaacaaaac ttaagttatt aagacaaatg aataaacaac tacgctttcc tcgaaacaaa
     8401 aaaataaagc acaaatgcgc atttacgcac tcatgcttta ttcgtacaat taattcagat
     8461 aacggcagcg cataggatgc caataacact tcctaaaata gctcctccta aaatgtcagt
     8521 aggataatgt aaaagtaaaa aaatcctcga ataagcgatc aaacaagcaa ataaaaaggc
     8581 taatattcct gcaactggac ttaattcgaa caacgcagct actgataaaa aggctagcgc
     8641 tgtatgtcct gaaggaaaag aagaagtctt cggtttactt gtgtaaagga caagccgacg
     8701 atttttttca taaggtcttt tccgtcttgt cgtttttttg ataaaaagca aaccaacaaa
     8761 aaaatagatc aaactgccga taagaatggc tagaccatat tctctagtat caggtatcaa
     8821 gactaaaaca gcagcaagga gcaaccaaat aaaaccacca ttagagatat tactgataaa
     8881 tttcaaaaaa ttgatttttc gactatccgt tttccgttgc ttggcgaact tgttgataat
     8941 actatcatcg attttagcaa tcaaacgcaa catgttattc ctccttttta agaacctttt
     9001 cttgcgtacg atggataaaa agcaggactt gaaaataaaa caagaaactg aaacctaata
     9061 aagcaaaata gattggtcct gcaaaccaga atgcgacaag aaggatcaac gtgcctgcca
     9121 aatagaaaag cgcgcttttg aaatcattaa aaaacaagaa aaatgcagta atccaagaag
     9181 cttctttttg ttccagttga agcaagattt tgtaactaaa gagaagcgcg cttaatagaa
     9241 gcaaaaacaa cgtagtatac atcaatgtat agccaattcc cccaccccaa tagcggaaaa
     9301 tctgataatc gatcaataga agaagaaaat aaacacttgc taaactatct tgctttccat
     9361 aaaaagagaa gttctcacga aactttttcc agaatagtga accgagcttt ttctcttcat
     9421 tttttttgta atgataaatc atatacagaa ctgttcgact tgcagttcct aatgtgaaaa
     9481 caggcaagac gcacaggaac catagtagct gtaaatggat aagacgtgga atccattcaa
     9541 tgatttctaa gaaataatca ttggcttttt taccaatcga tcgattcatc atccttttga
     9601 acctcctgtc aaatccatcc cttcaatgaa ataacgttgt gcaaaaaaga acagcaaaat
     9661 cgtcgggatc aagacaagtg ttgctcctgc catcagataa ttccattgtg tcgttgcttg
     9721 gttctgaaaa acttgcaagc caatctgtaa cgtgtatttt tcttgatctt ggatatataa
     9781 cagaggacct aagaaatcat tccaagctgc gttgaaggaa ttgatcgcaa tggtaatcaa
     9841 ggcaggtttc gtcaatggaa gcatcaaatg actccaaata tacaaatgat tcgctccgtc
     9901 gatttcagca gcttcgatca attcattatt gatcgataag taaaattgac gcatcagaaa
     9961 tatattgaac gcattgccga agaacgatgg aacgatcagg ggcaaataag tacctaccca
    10021 gccgatcttg gagaataata catattgagg gatcatcgtt acaaaccccg ggatcatcat
    10081 cgtagaaaga accaaagcaa acatcagctt tttcccagga aaatcaagct tcgcaaatcc
    10141 ataagcaata aaagaatttg aaaggacatt accgatcact actagcacag tgatgaataa
    10201 tgtatttctt gcatagcgcc aaaaaggaaa ggcggcaatc gtatccagat agttttccca
    10261 tacgattttt tctgggaaaa aggtcggagg ataacgaacg atttcttcca ttggtttcaa
    10321 tgaagtaaac accatccaaa gcaatggaat gatcaaaatg attcctatta ctgtcatgag
    10381 gacataactt agtatttttt ttgtttgccg attcacactg gtctttttga aataatccat
    10441 catttgccgt cacctccaga ataatagacc cacttaggtg caagcttcaa attgacaaac
    10501 gtcagaacca acacgatcag aaacaggatc attgacatag ccatcgcata acccatatca
    10561 aaagctacga acgccttgtt ccacatatgc agattataaa aaagcaaaga atttgcaggt
    10621 ccgcctgttc catttttcgt catgacataa gcttcttgaa aaatttggaa agaaccgatc
    10681 gtagaggtga tcacatcgta aaaaataata ggagtgatta atggcaaagt aatacttttg
    10741 aactttcccc aaacgcctgc tccatcgatt tctgctgatt catataaaga tctaggaaca
    10801 ttttgtaatg tagctaagta aagtagcatt gctcctccag aactccatat tttcatcaaa
    10861 atcaacgcag gtttcgtcca ttgtggatca aacagccaag ctggtccagg gatatggaac
    10921 cagcccagaa atgtgttgat caatcctgca gatggagaaa gcagctgcat ccataagatg
    10981 tagactgcta ctcctgataa gactgccggt aaatagaaag ctgtacggaa tacactgatt
    11041 cctttgattt tttgattgag tactgtggct aaaaacacac tgaaaatcgt cgtcagcggt
    11101 acattgaaga tgacgaaata aacggtgttg tataacgatt tccaaaataa atcatcgtga
    11161 acgaacattc ttttgaaatt tgctagacca atgaaattca tcttggatgt caaattataa
    11221 tcggtgaaac taccaataac tgaaaaaagc ataggcccta gcataaatac caagaacccg
    11281 ataataaaag gcgagataaa taaataggcc catcttgctt cttttttctt tcggatagat
    11341 aaacgcttca aattttcttg cctcctctgt acctagacag tcaaaaatgg attattgatt
    11401 ttgcttgact aagttttcta ctgctttttg tgcatctgcc aatccttctt ttggtgattt
    11461 tgttcctaaa agaatctggt caatctgttc attcactaat gtcgtgtaat ccggagcatc
    11521 taaaggaact ggtgtgatca ccgtattttt aaagttttcg tccgccattt gatagatcat
    11581 ttttccttta tccgttaggt tgtcattatt cactaaattc tcattggctg tcttgctcgc
    11641 catgatatca aaactttttt cgccgaattt ttcttgtact tctggagaag aaagatattt
    11701 gatgaattca taagaggcct caggatcttt tgctccgtat ggtacttcca agacgaatcc
    11761 gccaccccac gaccaatggc ctgaaccttc ttctttttct gggagctgca tcacgccaaa
    11821 atcaaaatca tccggtgcat tttccatcaa actagagtaa taattgatat tttgggcacg
    11881 catggctact aatccggaaa taaatggatc agatacacca gacccaaatt cggcttctaa
    11941 tttattgatc gtatttttcc cataataatc ttgccagtct tggatccact ccaaggcttc
    12001 cactttattt ttagtatcaa tcttgacttt ttcatctttg tcaaaccaac tagtcccgtt
    12061 atctgcgttt agcgcccata catcagcccc taaattccat aatggataaa aaccgatgcg
    12121 tgtaaattct tttccttctt ttttatctag tttacgtgct gtttcttcta attcatccca
    12181 agttgtcggt agatcttgtt cactgattcc agcttcttta aataactttt tattataaaa
    12241 aatgacttgt gtatctgtat tgaatgggat cgcataagga tcaccttcat attctactgt
    12301 atcccaaagc tgcggataaa aatccttaga agtgtctttt tctacatatt tggagagatt
    12361 agttgcttgc tgtgcttctg ctctttgggc aacggaattt atgtcctgta caataacatc
    12421 gggtgcattt ccagctgtta ttgcagataa agatttagtc cagatgtcac cccatggctg
    12481 atacttataa tttacatgga tctcgttttg tgattgattg aaatcctcaa tgatttcttc
    12541 gatagcttct tttcgtgcac cggatcccca aaaggaccag aaatcaatat tttttcttcc
    12601 atcacttgtt ttttcagaag aatttgctgc acttgaactg gcatcgcttg cattacttga
    12661 tccgcatcct cctaataaaa acaatgctga acttgccagc aaccctgtaa acaataattt
    12721 tttcatattt tacctccaaa ttttaatgta accgatgccg gttttaatat aacgatatca
    12781 ttttgttctg aaaataacaa tttataaggt atgattctaa aaaaattatt aatgatacca
    12841 atactttata taattcgcga gcgctttctt tgaatttttt aatctttcgt agcagaatga
    12901 gaatgtatta aaaatagatt aggaggaatt ttgatgttta gacaccagaa agaattgcag
    12961 tttgaagcaa aaccagatcg tccgaatcca ttaatcgcga aatatctgca ggagctgatc
    13021 ggcggccaat atggcgagat gagtgtagct atgcagtacc tgttccaagg ctggagctgc
    13081 cgaggagaag aaaaatataa agatatgctg atggacattg caacagaaga aatcggccac
    13141 gtggaaatgt tggcgacaat ggttgctcgt ttattagagg gtgcgcctgt tgaagatcaa
    13201 gaagcagcga tgcaaaaaga tccaacaatc ggtgccatta tctctggaat gaatccacag
    13261 cacgcgatcg tagctggctt aggacctcgt cctgctgaca gcgtgggcaa tccatggagc
    13321 ggtgcctata tcattgcgag tggcaacttg ttagcagact ttagagctaa tttaaatgcc
    13381 gaatcacaag gaagacttca agtagcacgt atttacgaaa tgatcgatga tcgtggcgta
    13441 aaagatatgc tgtctgttct ccttgcaaga gatagctatc atcaaaatct atgggaatgt
    13501 gcagtgaaag aattagaaga aaaagaaggt tcaatcttag tcccaagtac tttcccgcgt
    13561 gacaaagaac gaaaagacat tgcttatgac ttctataact tctcagaggg agaccaaagt
    13621 agtgaaggtt cttgggctag tggcaaagca ctggatggcg aaggcgaata tcaatggttg
    13681 aaagaaccta agatcgaagg cgctgcacca aaattagaac cagtaacacc aaaaatgtat
    13741 gggacacctc cattcaagaa ataaatatct acccgtagaa acacccgttt tttgttccac
    13801 acgctttgtg agcaaaactg actaaagtca ttaatcataa ccacccttca aaagggtggt
    13861 tttctctaga gctataagct caaatcaccg gccagcgcct aaagacgctg gctttcatat
    13921 tttatgttca agccatattg cctttgccag ttttgcccac ctcttaaaag gtttatgtac
    13981 tactccttag cccttaaaag gatcgtctta ctctcgtaca ctcagcttat ctaacgcact
    14041 atcatgtttt tcttgttctt gaatgtactt tttgatggtc gcttcatttt gtcctacttt
    14101 tgtcacatag tatccttcca cccaaaagtg tcgataccca aatttatatt ttagatttgc
    14161 acaatctctt gatgatctcc tgaatattgg ctcgatattg attataaatt atttttcttc
    14221 tatactttgg tgtgaataca atgtggtatt tacacatcta ctttgtacgg gctaaactat
    14281 ttccctttat tgctccaccc gcatagcaag tggttttgta tttcaatcgc tcggcgcgct
    14341 caattaggtc taaagacaat aaatctaaaa acccgctgct tcactgacag aaacaacggg
    14401 ttttcagaaa aaaatgaaat gaaaaagatc tcttacggca atctatttta tttttttgat
    14461 tttttataaa tggaaaatgc ttcaagaagt ccaagaaata aagccgtata aaaacaagaa
    14521 cctatgaaat ctttatttat tagatattca acagaaatcc cgataacact agccaacaat
    14581 atatttatca gaagtgtaaa ataaggatgc tttttataga atagaactag tgttgttttc
    14641 atagactctc ctttcttcat atttccaatt aatcacaaat tcacggcatt actagaaaaa
    14701 ttaaaggggc gcagaagtgg aaaaatactc ttcgactttc cctacaaacc aggccatctt
    14761 gtgtttaata actataccaa atctaaggta cctctaaatc taaattccat ttgtggcggt
    14821 gacctccaat ttttaactct tctatctccc atccagtata cataaccttg atacttcgac
    14881 cacttatata tcgaacacac aaatacctat ccattgcagg agtactacct aacttagttg
    14941 ctacttgcat ctaaataaca gctgaaaaga taggacttac tcttagtaaa gtatctgaac
    15001 tattaatttt taaactatca tttttatcct caactacatg cacatctgtt gcaacttcac
    15061 tttctgtctc atccagataa atgattaagg aatcgggaat tgcaatatct tcagctgacg
    15121 ctggaatagt tgagctaaat tagtcatcat atctttatta taaaataacc aacagcaaaa
    15181 aatacaatga aaaacaaact tggaactaat gtatcattaa taaatgttga aaaattcagc
    15241 aactcattac tgtatgattt tagcagccca accaacatat accctataaa agctaataag
    15301 atggttagga atcctttaga atttttactc attcatagac acttccttaa ataactttta
    15361 ttatagaagg tattgtaaat aataatattg acaaaaagtg actcaaaata aaaaaaaact
    15421 tacattatac tggaatgaac tgtattgata gtgtacctaa aagctctact gacataagat
    15481 cgtgtaatcg gtaaagtaat tttattaatt atacgggatg taatcaatta gagaataaac
    15541 taatcataaa aggtactttt acattcataa ttgtcgcttt gagattctac tttggttcac
    15601 tatctgaccc aattgcacat tttcgatcat tacaagtatt ggtgtcgtta gaacaactaa
    15661 ataaaaacta gcggtagatt tttcagaatc tatgctaatt tctgtatgga tgttccagtg
    15721 atggcaaatt tctcttttca ttgtttttct tgtcgcatga tatgcctttt tgtgttattc
    15781 agttaaacta tagaaattct ttcctagaca gatcttgatc ccaaatttta cgtttttgag
    15841 catcgatata gtttaaaatg acattactca tatctaaaga gtttagattt tttaggacta
    15901 aaactgtcag ttgttctttt tcttctttct gttaaagtat tgtcgcgact tttcgtatac
    15961 gttaagatat gtcgtgaaaa aacaagtttt gtgacacaaa aaaacgaatg acttgaggaa
    16021 atcacctcaa tcattcgttt aaaatgctat atattcttta gtttaagata cggaaattaa
    16081 tgccatcgac cacactagta gaatgaacga catttacttg gccttttttt aatttcgtta
    16141 tgtttagcat ataaaaatat tatagagttt acttacaatg cttaaaacat atactgtata
    16201 atctgatcaa ttttagccat gtcttgcaat ggtaagtttt ctattttttt gagttttcgt
    16261 tccttaaaat caagagattt taattgtgta ataattactt gtccttttat atctagctct
    16321 tcaggtaatg agtaacgtgt cggtaaattc tttatagtgg aagtaattgg acatatgacc
    16381 gaaaacattg ttttacggtt aaattcataa cgagagacga ctagtcctgg tcgtcttttt
    16441 tgaatttctt ttcctacgga gggatcaaaa tcaatccata caatatcacc ttttttagga
    16501 atataaattc tttcttctga cactataaaa tttctctccc ttcaggagtt aaatcttccc
    16561 attcgtcttt ctcataatac tcggcctctt ccccaccact aaatggatct tcaatttttg
    16621 gaactaacgt aattgttcca tcatcagaat acacaacgat atattcttgg ttttctgaag
    16681 gtttctttcc gttatcagaa ggtaaagtta ccactacaga acttccttgt aatctagatt
    16741 ttgtaacaag cattatcatc actcccttaa taaaagtata ctatataaca ctaagtaacg
    16801 caaaattccc ctttgtaaac tatctagaaa attgttttgt ttaaagtata tcttattgga
    16861 tataaataga aataaattgt agccagttca ttttttcttt aactaaaaaa ataatccaag
    16921 aaacattgaa cctatggatc caaaagctac ccaaatacta tagctaaccc cccgctacct
    16981 tcaattttgc agataaaacg ctttgggaga aaatttcaaa gacaacaggc catcctatca
    17041 tgcccgatta taaacaaaaa gctggctatt ccttttgctc tgacaaattt caattgggaa
    17101 agttcacgaa actagcaggt gaagaagttt caactgttcg gatcaaagaa tgcccgcttc
    17161 aaatagaaac attggttcaa cgaatttcaa ctagacaaga ctttgctatt gtagaacgtg
    17221 agatcgtagc tatttttgta gaagaaggga ttttacatga tccaactcat atcaatgtgg
    17281 ataaatggca acctcttatc tataaattca gagaatatac aactgccaac cagtcgttaa
    17341 ctttgaattt tagattccaa gaattcaaag agtcataatg ataaacatcg ttaggtataa
    17401 ataaaaaaga ctcgcagcac gagtaaaaaa caaatagaag ctatacatta gaaaggtcga
    17461 ctgtgaccct atagatgcag tatatgcagc ttatgagcat ttcttctgtt ctctttgttt
    17521 ttgggatctg tgctgaagtc tttttcgttt tttgccgctc tttctttgaa aaatcgatta
    17581 tgttatacct tctccattac taattcaggc atctgctgag taatgatttt tcgcgcaaaa
    17641 gaaatatctg ttacccaagg ttcgatttgt tcttcagtca agacaagcgg cattcggtca
    17701 tggattggtg cgactgtgcg gttaggttca gtagtcatta tgattgattc tgccattttt
    17761 tcatcctgat ggctacgata aaaaccgcca atatacacta ttttttcctt atttgaaaac
    17821 ataaatttgt ctttttcttc attccactca ttgggaaaac acaacgattt tgcaaaaatg
    17881 gttgatgaaa ggtctttttt tcttctactg tctctgcacg ggcattaatc atcaattgtc
    17941 cttttttaaa tccggtaaat ccccacttca tgatttccgg agtgaggatg ttttcttcct
    18001 cttttcttaa cgtcacaaca agatttgacg gaaaaacttc tccagctgct aattctgctg
    18061 cctgcttcgg ttgtttcgtc tgggcttctc gataataatt ctgaaggagc tctgactcta
    18121 gatcaaaaaa ataacgacca cacatcctaa tccctcccaa tctttttttc tatcatacac
    18181 tgcaacaaga agttcgcacc aacaaaacgc tttcaatttt tgtagaacta aaaaagttgt
    18241 gacaagtgac aaactctcca gtgaatggta ttctcctgcc aactcctcgg tcacaagtca
    18301 caatattcaa caaacatgag aagctgcttt ttgaaattgt tccttgtgac tcggagtgaa
    18361 acggcaggct tacgacctct gggtaaacgg tgttcaaaag ccagtttctc ggtattaagc
    18421 caaatttatg caaaaatgta gaaagctatt ttcacaaaat ttttcttaat actcgaaact
    18481 aagcgacttt ttcacaacct cttccttaaa cggtgttcaa aagcttgcat ctgcggtaat
    18541 aagcccaacc aaacgaaaaa tatgagcagc tatttttatt tggctgttct tattacttga
    18601 tgcagggcag ctttttcaca acctctccac taaacgatgt tcaatcagct aaccttcggt
    18661 attagctcaa aaaacggcaa aatatgagga actattttgc cgtttgtttc tctaatactc
    18721 aggtcaaagc gctgatttca caacctctaa tacacggtgt tccagaagta gcttcttcga
    18781 aaaataagcc gaaatttcac aaaaatttga gaagcaattt tcgtaaattc cttcttattt
    18841 cttgaagctg accacttctg tcacaacctc taattattaa agaacctttt gcgaagttaa
    18901 caatctttct ttgaccaatt gaaaattctg cctaagtagt gatagatcct gtgtgacttt
    18961 caacaataag atacttccct ggatttcagc aatcaacgct ttggcttgac ggtctgcttc
    19021 ttcatcggac agtttttctt cagaaagtgc caggatattt tgtttcgtat acttgaccca
    19081 ttgattgact aattctagta gcaacagacg gtattcttca tccactgtac tcaactcaat
    19141 aatcaaatta cctacaggac agccatgata agtgatctgt gtctcatgat cagcgaaaat
    19201 ccagtccaac atttcttcta atgcttcttc tggctgtttt ccagcattta agatgtcttc
    19261 gaacaattct gtctgccata cgtccatatg cgcatggatc accgctagac aaacttcttt
    19321 ttttgaagaa aagtaataat aaaattgacc ttttcctgta tcagaagctg ctagaatatc
    19381 tcggattgac gtttcatgat agcctttttt ataaataaga tctttggtcg cttccaagat
    19441 cttttccttt gttttcaatg atattctcct ttttcatttc tcatagtcct atattataca
    19501 gtaaattcat cattttttct tcttaatcgt ttaaaaaaga ctgtgccaaa ttatctgaca
    19561 cagccctcac tataaaactg ttctcatata gctagatgtt cattgattca agaactgcga
    19621 ggacaaaagc aattcctatc ttatgatagt tgctctaata cttcctctgt tgtcatcact
    19681 tgatctgcaa taaagccaaa gttcgtcaac gctgcatcca ttgcttcttg gccaggagca
    19741 ccaattgcat ctgtcgctac cacaacattg aatccactct ctaccaattc acgcatatgg
    19801 gaatcgctac acaagtttcc gttcattcca ccaagaatta ctgtgtcgat tccatttttt
    19861 ctcaattgga gcgccaaatc attgctttct ggtccaaaga ttttatgagg agagctgatg
    19921 atcgtatttt catccaaata aggtttcaat tcttcgataa aatcggctcc tgaaccttct
    19981 tccactgatt catattgact ttttctagca aacatctgct gctcaagcat catatgttct
    20041 cctgcaccgc caaattgcca tttatggtca tgtgggtaaa aataatgagg agaaataaag
    20101 agtttgtatc ctttttcttt tgcttgtttt accatttcaa tcatattttc gatcgtattg
    20161 tatttttcta agatatcttt tgtcaaacca aagccaccgc ctgctggttt caagaactcg
    20221 acttgcgggt cagtgagaac tactgctgta tttttattat tgaacatggt ggattctcca
    20281 ttcgttattt attttaatac atcatttaga gcttcactca ttgtcggatg agaatagatt
    20341 tgatctctca ataacgtata aggaagtttt ccattcatag ctagcgaaat caagttgatg
    20401 acttcatgag aatcttccgc atagatactt gcccctagaa tcaagtcagt ttcagggtcg
    20461 actaaaactt tgaatacacc tttaggatct tctaatactt gtgctttagg gacaccgctt
    20521 gtcatatact tgaataattt gtaattgacg ccttgtgctt tggcttgttt ttctgttaaa
    20581 cctacattgg ataaagtcgg agtgataaat acactgtaag gcacagcttt ccggttattc
    20641 gttgttcgtt tattctcgcc cttcaactga tccataatga ttcgataatc atctagagag
    20701 atataagtga actgcaatcc gcctttgacg tctccaatcg cccaaatgtt gtctgcatct
    20761 gtacgaaggt attcatccac agcgattgct ccgcgatctg ttagtttaac atctgtattt
    20821 tctaatccaa gtgatgctgt acttggtttt cttccagttg cagccaaaac ttttgacgct
    20881 tggattgtta cttcttggcc atcttctgta aaggtgacgg cagctgttga ttcttggtct
    20941 gctacttgat ctacagatac acctagatgg aattcgattc ccgcatttgt catgtcttct
    21001 aaaatcattt gcgcgatatc ttcatcttca cgtggaagaa attcagaatg ggcatcaaga
    21061 acgacaactt tagatccgta ttcattgaac attgatgcaa attccaagcc aatatatcca
    21121 gccccgatga tcactaattt ttccggtaat tgttcctgat ccatcgcttg cgtagaatca
    21181 atcaagtagc gacttgtatt caatcctttg attggtaaaa tgattggttc agaaccagta
    21241 ttgatgaaaa tccgttcgcc ttcaacaatc actttttcac cattattcgt cacttcgatt
    21301 tgatggttag aaagaaaacg tgctgtaccg tccatcactg tcccagttgc ctcatcgctg
    21361 atcatatgat aatttttggc gcgaagcatg cccgtcaatt tttctttccg agcaacagcc
    21421 tctgtaaagt cgattcctct ttctccattg aaaatcaatg atttagaagg aatacagccg
    21481 atgttgatac atgttccgcc atacatacgt gttgactttt caacgactaa aaccgattca
    21541 ccttttgaag ctaacgcctt cgctaaagtc ttaccgccct ttccaaaacc gatcacaata
    21601 ttttgatatt tttccatcat ttttccctcg cttctttttt acctaccgat tggtacaaaa
    21661 atatgataac cgataattgt ctattctcca aatcatctgc tttatttgtc aaaaaaaacc
    21721 agacaaaaag cggtcctttc acgtttttag tctggttcga tttcctttat tcttctaaac
    21781 aatcgttact tctacatcga tatttcctct agttgctttg gagtaagggc agaattcatg
    21841 cgcatcttct gcaatttttt gggcttcttc ttcgctgatg ccatcgatat gcacttctaa
    21901 tactacaccg atctgatagc cgccatcatc taaattataa agagagacac gtgcttttac
    21961 tgtggactct gctgtcattc ctgcttcttt gatcatatgt tgtaccgccc cattgaaaca
    22021 agaagcataa cctgctgcaa ataattgttc tggatttgtt ccttctgcat ggattcctgg
    22081 aggagtgatt ttcatatgca tcgagccatt tggtgcctcg acttctccat tgcgcccgcc
    22141 atggttgatc attgctgttt cgtatgcttt tttcattgta ctgctccttt cgattgacca
    22201 tttgctactt ggctataatt atagtgtatg acaagtaagg cgctttctac aaaaattctg
    22261 cttattcgaa taccagaatg ccgtcttcta ctatttttcc aaaaaacaaa aaaatactac
    22321 tcgtgaacaa cagcgtcata tttgttccaa aataaatagc aatcttttct acataatctt
    22381 ttatatgatt gattttaaga tacatatata ccagaaagtt gaccccataa accaatactg
    22441 ttaaaaaaat aaccatattc gttaccattt tttcctcctt ttgctggtct ccctaccagc
    22501 actcgattta accataccac tttttttgcc gtccaagcaa ctagcctttc aaaaagactg
    22561 gtttattctg ctgttaatga ttcaagatca gaaaaatgtc cgaataattt tgctaatttt
    22621 actaacgcca acttgttctt tccgaattca tcattttcta tctgtaatac tagtaacggc
    22681 tcatgtaagc ttaaacggat caaaaaccag ccgtttccgt atggctcttc tagatcatat
    22741 cgaatacctt cttcattttc cgggtcgaag ttcatccctt tttcttcttc caacagtcgc
    22801 ttcatttctt ctaatatctt gtttccttgt tcgcgtggtt catcgctgaa caattttaat
    22861 cggatttctt gtacttctac gggctgtttc aactctttga tttgttcatc taatgtgata
    22921 ccttttcttt ttaattctgg caaaagcatc aggatctttg cgatgacata tgcaccatca
    22981 tccagaaaat agttttcctt gaatgcagcg tgtccgcttg tttcgatggc tagctgtgta
    23041 tctttcccaa gacggttaag ttctaatgct ttattgatta catttcgata gccggaaata
    23101 taacgaactt ggatgcctcc tttttcttca atgaatcttt gtagatgaga agaagtaggg
    23161 gaattcgtca cgattcttgt tcctggatgc tcttttaata cgattgtact taatactgcg
    23221 atcaaattat ttcgattgat cactgttcca gaagcactga caactgcaga acgatccaca
    23281 tctgtatcga aaatgatccc aagatctgct tcatttgcta aaacagcctg cttgatactt
    23341 tccatcgctt ctttattatc aggatttggg atatgattag gaaatcggcc atctggttcc
    23401 aaaaattggg aacctgttga atccgcacct aagctattca atactttttc agcaaagaat
    23461 ccaccagcac catttccggc atcaacaatg attttccaac catttagtgg tttgctttct
    23521 agtgttccaa tccccttttt aatttttgtg actaaatctt gcgcataagg tgtcaaaagg
    23581 tctgcttttt ctacgcttcc agtttgttcg ctttttattt tttgctgctg tgataggata
    23641 aaagcaatat cttctttttc tgctcctcct tctgaggtaa agaattttat tccattataa
    23701 taatagggaa gatggctagc agtcagcata accgcagcat cgcacgagaa ctgttcaaat
    23761 tgagtggaca taaacatagc aggcgtagta gctaattcga aatcgatgac atgtatccct
    23821 tgttctttca gactttggat caacgcctct ttcagtgctg gtccgcttat cctactgtca
    23881 tgaccaatcc caatcgtcca ttttttattt ggatcggttg atttcttctt tttctttatc
    23941 cagtttgcta gtccccagcc gatctttttt accatgtctg tcgtcaagtt tacttcatat
    24001 ccttcaactg gtaatgcgat tcctcgaata tccgatccat tttgcagcgc tttcatatct
    24061 ttcatataaa agtcctcctc atttattccc tcattttttc cagcttttct gcaaatcgct
    24121 gtgcttcttc ttctgagcgg caaataataa tgaatgtatc tacaccggcc atcgttccga
    24181 caacttcagg gtaagccact tcatctagcc aattcgttac cacatcggca ccatttcgtt
    24241 cagtcaatac gataaccatg aactgaacag actggatacg caaaacctcg cgtctaattg
    24301 ctgtacgcaa acgttcttca tttaaagaaa cagatggctg gttaaataat gtatagcgta
    24361 ctattttgtt ttcattttga ctttttacta acttcatttc tcgaatgtct cgagaaatcg
    24421 ttgcttgagt cacatcggcg cctgcttcat gcagccgcgc aagcagctct tcctgtgtcc
    24481 caataggttg ttctgtaatg atctggcgga tcagtgcttg tcgctgtttc tttttcatat
    24541 tagacacctc tcaatgttga ttatagcaaa agataagtaa tatttcttca tattttggta
    24601 ttttttactt ttattttttg ttttcattca ggttgctaac ctttttctat tttaacacgc
    24661 aaaatcgtct tcattttttc aggtgctgat tttcttggat cgctgatgta aatctctttg
    24721 tgttctttag aagctctttc atagccttct tttgcgatga atgcttctaa tttctcaaac
    24781 gttgctggtt cttcggcata gggcccgacg tgtaagattt gggcacaaag accttcttcc
    24841 atctttttga atactacttg ttctttcaag tcttccggaa gtttgctaga acgctgaatg
    24901 gcttcttgtg ctatcgcttc tgtaacaaag tcaggctgtc ggatcatcaa ttgataagaa
    24961 aaatgttctt ttttcatcac tggttcatgc aaatactttt cttgcagtcc ccattgtcct
    25021 tccagtggat agacagtata catctgataa tcaggaatca gccaattttt tttaggcgac
    25081 atgcgtattg catagctgac tgcatataga caggctactc ttcgtttaaa gtcttcctga
    25141 ttcgggtcgc cgatccctct gatgctaaag aatgattttt taggaacctc tagtatttgc
    25201 gggctttttt tcatactata gctcttgtct ttacgccatt caattttttc catttttctt
    25261 cctccgcgaa attccattta gttatttatt atcatacctc tttttgagtg acttggctaa
    25321 tcgtgttgac actatttttc agtttgtgct agaataagtg aagtaaataa atcgtacaga
    25381 taacacgttt cggcgtgtcg agagagaggc ggtttctatt tttataatag aaatcactct
    25441 ctttttttac gaaatttttt agaggtgatg taaatgacaa aacaaaaaca gctgctttta
    25501 gtacctgcag taattgcaac agggatgatg tcttttgcgg gcgtattgat cgagacggcc
    25561 atgaatgtaa ctttcccaac tcttatcaga cagtttgatt taacgactgc acaagtccaa
    25621 tgggtaacaa cgatttatct tttgatgatt tcgatcattg ttccgctgtc tacttatttg
    25681 aatcgcaatt ttagtctcaa aaaactcttt ttagcttcaa atctgctttt cttggcaggt
    25741 ataatgatcg atttcttctc tcctaccttc atggtcctat tgtttggtcg attgctccaa
    25801 ggtgcagcga caggtatcgc tcttccgttg atgttccata ttatcttgac atttgccccc
    25861 ctcaatcgac gaggcgcgat gatgggtgtc ggtactttga caacggccat tgctccagca
    25921 attggcccca cttatggagg cattatgact tcacatttta catggaatca tatcttcctg
    25981 tttcttgtgc ccgttttgat tctttctttg attatcggct ggtttgctat tcccgagatg
    26041 gcagtacaaa aaagtggtta tttggattgg ataagcgttt taggaatttg tcttttgttt
    26101 agtggaagtc taacattttt gagtacgttg ggaacagttt ccagctggat tgctttaatc
    26161 atcggcggta ttggctggtt ccttttttac tatcgaagta aaaaagtgga gcatccactg
    26221 atccgactgt ctattctttc aaataaaaca ttccgcttgt ttttgattgg cttcttagtt
    26281 tatcagtttt tattacttgg tgtatctttt gtcttgccga atttccttca gatcgttcaa
    26341 ggaagtagtc cttttgtcgc tggattagcg atgcttcccg gcgcagcggt tggtgctttg
    26401 ctttctcctt tttccgggaa aatgctggat caatatggtc cgaagaagcc aatttttgct
    26461 gggctgattc tttctttgat tggttggcta gcattattcc tactcatagg acacgcttca
    26521 gtgggtgtat tgattgcttg tcatgtgttt tatatgatcg gtatcggatt atcttacagc
    26581 aatatgatga ccaccggaat gaatgcactg aatgaagaac tacaaggaga cgggaacgca
    26641 atgttcaata ccttgcagca gttttctggc gctgtggcta cttcattagt agcggtcatt
    26701 atcaattacg tccagcatca tacttcccat aactatgaag tgtctactac attaggatca
    26761 aaagcagcat taggcgtctt gttgctgttg cttctcgtaa gctttgctcg attcgcctat
    26821 tatctgttct ttgctaaaaa agcgtagatt ccatatacgg caggtgttgc atctgccgtt
    26881 tttttgtgtg aaaatggttg tttgtaaaag gaggcttttt tatggaaaaa attgccatta
    26941 tcggtggcgg gatcattggt atgaccctag ccagttactt agatacatca aaattcaatg
    27001 ttgctttatt tgatgaaggg acaggacagg caactaaagc tagcgctggg attatctccc
    27061 cttggttgtc gaaacgcaga aacaaacaat ggtatcgtct ggctaaagac ggcgctgctt
    27121 ttttccctaa acttataaaa gatttccaac ttgatcaaac gatttacgca caatcaggga
    27181 cacttttgct tcgacctgaa aaagatttag ctgaattggc tgttcttgcc gagaaacgaa
    27241 aagaagaagc gccagaaatt ggaaacatcg aatggctttc acctgaacag acatctcaag
    27301 cacttccgtt attgaaacca gcagtttcac tgcgtatcag tggtggtggt cgtctggatg
    27361 gaaaagcata tttagaacat cttggtcagc gtttagcaca aaaacaaatg gagcagatcc
    27421 aggcaaaagt cactttgcat aagaagagcg attcttggct catcaaggga ccgtttggag
    27481 aatacacagc tgatcgtgta attttgacac ctggcccggc acttaagcct ttattacaag
    27541 aattaggcta tgctgcagat atacgtccgc aaaaagggca gctcttagca tttcagacat
    27601 cttataagat gagtcaagac tggcctgttg ctatgcttga cggagaagct gatttcattc
    27661 ctttcatgga tggaaagatc cttttaggtg ctacccatga aaacgatcaa ggctgggatt
    27721 tggctcctac tagcgaagct tataagggat tgactaagag tacagaacag ttcttgactg
    27781 atccagatca gataaaaaac tggccatacg agttccgtgt aggtacccgt gcttatacgt
    27841 cagatttttc cccatttttc ggcgtactgc cagatgatcc aagcttactg gctgctagcg
    27901 gtctaggctc ttctggcttg accacaggtc cttatatcgg ctatctatta gccaacttct
    27961 taaatactgg tcattggcct acggaagatt atcaaaagga catctccacc tatattacga
    28021 aaaatccatc atagaaaaac agctttttca agtaaggatc ccgagcgata ttgcgcatgg
    28081 attacttctt gaaaaagctg ttattttagt tcaatttctg aatcaaattc tccaaacttt
    28141 cttttgcttc atctggcaag agatcttttc ctatctgagc ttcaatgaat cgtaaacgaa
    28201 tatcaagtcg ttgtttcacc agatttttat aggacgtatc ctcaaaagaa acaggaaaat
    28261 ctggtatagg taaatagtgg atgtcttcaa atggaccaaa cgacttgaat tctgccgttt
    28321 gttgcacgat tctttcaacg attgacaatg tcgtttcagg tttgattttt ttatcatcga
    28381 aatatccagt attcaggaca tagcaggctg tttgctgttt caaaaacaac tctcgaaaag
    28441 cttgatagtc ttccctaaga ggatagacac ggaaaggatt agcaaatggt tcaatgacta
    28501 gctcatctag ctgtcttttt gtagtgatat tttccgcgtc tgatcgtttt gttgctaatg
    28561 ttgcaccaaa agacgctgct aaaactgggt cattgatctt gataattggc ggaagcgaat
    28621 catctttcat gatccaaaaa acagcatcga tcttttcgct aagatgatcg actcggtcgg
    28681 gtgtcgcata acgtgatttg actgtccgcc catttccgtt tcgtatatct cctgttacta
    28741 ggactttttc tcctttactt gtacatgtca cgcccacatt ctgacaagta aggaaagaag
    28801 aaatggccgg atcatcagac ggatagtctt gtgttttatc aaagtaagaa ggttccaagg
    28861 cagtagcaaa accatttttt cgagaaataa cgaaagcatc gtcatgcaag atcgaaacag
    28921 agtgaccttc cgtatgtttt gctaaggtaa tcgttgattt tccagaaccg gacaacccaa
    28981 atacagccat cgtataggtc tggtcattac tagcatattg tttcatccca ccgtggcagg
    29041 caataaagcc attgcgattt cccaatgccc aagcaagcgt caaagtcgct ttttttaatt
    29101 ctccaaagta acgtaatcct agcacaaccg ctgtattatg gagagcatca aacaaaatga
    29161 gtccatttgg aaatgtttca tgtgtccatt cgggatcaca aaagatataa agatctccct
    29221 catcaaagga aacagacttt tgataagtct ctaagatctt cgaattcgct ggctgaaaat
    29281 tcagcaaata agaatacaaa ttgttctcaa atccttctgg aatcagcaaa tgactttgca
    29341 cagaaaattc ttcagatagg ccaacgatta cttttgtatg gtaaaactgg cgcttgcgac
    29401 tttgatacac agcttctctt aaaattttct cgtatttgct ttgttccttg tctaagtcct
    29461 caaagatccg ccgagcactt gctgttcttc ccacaatgct tccatcattg aatactagtg
    29521 tcctaacatc agctggtaaa tctagttctt cagcatgtga gattgttaaa tctgtgatga
    29581 tcgttcctgg actccctttt gctagttgat aagctgtctt cctatcaaaa acttcatgaa
    29641 cattattacc gtaaaatgtc gtttctacaa tcgatcgaat actggataac agtggatttc
    29701 cacttgtgat ttcgtttaat tcgagactct cttttgtact catggctcct cctcattccc
    29761 taaacaaaaa aacgttttct ttattataca ttattcgagt ttttatttgt actgctgcgc
    29821 tgtgggaagc aaacgagtta tttttcacac aaaaaaaccg agaacttgaa taaacaagtt
    29881 tctcggtttc atcattttcc ttttatcggc ttagtaccag ccgtttgcta accagaatgt
    29941 tttagcagca tcccatgaac cataacgaga tgttacatat tgttctgcta cacgttcttg
    30001 attggcagct gaatagtcgc cgtttaagta tgaagcatct aattgataac ggccgatgta
    30061 acgtccgtta gtagctgtgt atgaaccgcc tgattctttt tgtgcgatcc attcttttgc
    30121 agagcttgaa gttggcgctg ttgcagcagc tggggcagca ggagctactt caactgtttc
    30181 aacaggtgca acgtatgtat gttcttgcgt tgctgtttca gttgtagatg ctgtattttc
    30241 tgttgttttg ttttctgttg ttgctgaaga atcatttgaa gttgggattg ttaattgttc
    30301 accaacataa atgcggttga tgtttgcgat cgaatttttt tcagcaattg catcgatcat
    30361 tgagttgtct cctgcaaatt tttgtgagat cttagataat gaatcacctg attttactgt
    30421 ataaacttca tcagcgtgag catttgtagt tcctacaaac attgcagtag caccagcagc
    30481 aaaagttgta cctaaaacaa ttttcttaaa tgaattcata ttaaactcct ttttttctcc
    30541 gttttttatt ctttcgacgc tattaacttt atcatttaga aatatttcat aggtaaaaga
    30601 aacatgacat ttaatgacaa aataacgaat tatttaatat ttgtaacatt taaacaaata
    30661 tttttttgat acaaaaccat aaaagccttg ttgtataagc aaagtaatcg tttttctatg
    30721 ttttttttat ttttgaatga aaaacgcaga ttctgtaaca ttcactgcaa taatccataa
    30781 aaaaaagcaa aaaaattttt gatttttatc aaattctctt aaaaaaatgt aaaaaataca
    30841 aaaaaaccag taattgttac aacaattact ggacaaaaac tttgaataat gttaactatt
    30901 tttttatttt aactttctcc gcaataattt ctgggcatgt tccaattcgt ttataacgac
    30961 ttcttctgtt tctttagcta atatttcatc taatacatca agccattttt ccggttcttt
    31021 tgttcctaga cgtccaatag cccaagctgc tgttcctctg atgacagggc ggacatctgt
    31081 taaacattct gaaatctcag gcaatgccgt tcgatcacct agattagcta aagcaatcaa
    31141 tgcgtttctc tgcaaaggtt tcttcccacg ccatgaacca gctaaatgcc caaattgctg
    31201 tttgaagtct ttattagaca tcgaaagcat tggctttaat tttggatata tttcgtctac
    31261 ttttggttcc atctcttcat gaaaatgaaa atcttttcct tgattgtaag ggcagactaa
    31321 ctggcagata tcacatccat aaatgacatt tcgcattttt ttacgatatt cttccggcat
    31381 catccctttt gtctgagttt gataagacaa acatcgctgg gcattcattc ttccgtcacc
    31441 aagcaatgct tttgtcggac aagcagtcac gcagcgcata cagtcaccgc aaccaaattc
    31501 tcctggttta tcaggctcaa atacaatatt agtaacgatc tcaccaagat agacaaatga
    31561 accgaactct tcagtgatca acaatccgtt tcgtccaata aatcctaaac cagctcgctg
    31621 tgctacagcg acatcgatca gttctccagt atctacttgc ggagctagac gccattcctc
    31681 ttctttttgc catttttctg ctcttgtttg gatgaattcg atcaatcggt tcaaacggtc
    31741 tctgagtata tcatggtaat cgatccccca agaagcacga gcaaactgcc cgcgtttttc
    31801 atccctaggc acttcttcat ggatcctagt aggatacgca agtgcaatgg aaatgatcga
    31861 tttcggctga tcaaatgtca tttctggata aagacgctca tcgatatttg gatgttcaaa
    31921 gccagaagta tggccagctg ctttttgttc ttccaacgat tcttttaatg aatcaaaagg
    31981 ttccgccgtg gtgaacccga ttttgtcaat tcctaatcgt ttgctttctt gtatgatttc
    32041 ttcttttaat gtcatggtct tcctcgcttg ctgttaacgg ctgtataacc cataatatag
    32101 tttttctttt ttcagctgga caaactggat ctgctgatcg gcattcttct tttcttccat
    32161 ccattttttt cttgcagcaa atacttccca ataatttgtt gaaattaatt ttcttttgtt
    32221 atctaatgca ttaaaaaaat tcaatgaaac tggttttgta cttaacagtt catggagcct
    32281 tacctcttcc catggcgaaa actcagacag atcgcgggaa tacaatactt cttcatagag
    32341 atttttttgc tgtgtgtacg tgaatgtttg cgggattttc ccaggcctag caatttctac
    32401 tagacgatta taaaacatat tcattaattt gctgttggca ataaattgac ttttataata
    32461 ggccgactgg atctttttga ttactccttc tgctttgatg actgaaaata atgcaggcca
    32521 tgctttgacg gttatttcgt cgttttgttt aactaatacg ccaggtgtga agccgactgg
    32581 agaaaggaac acaaaataac cgataccggt attttcatct aaaggctgaa attcatcaaa
    32641 caaccatttg cctatgtaca gctcttcatc tggttctgtt gtctcatttt gatttgctat
    32701 gaataattta gctaattctg cttcaagaaa ttgaaggtct tgatccttgt cggtattttc
    32761 ctgctggctg atcaatcgaa agacttcttc tttaatcgta tatttatctt tacgtgtaaa
    32821 attcatagcc acaccttcct tccttactct aactataacg gaatttcttc aaaaaaggta
    32881 tctgtacgac gaaaaacagc ataatttttt ttcatatctc ttataaaaaa tgcaaggtct
    32941 gctcctcaag ctagaccttg cacttttcag ctttcttttg tttgtgacaa accttcatgg
    33001 atcgtttcaa tattccattt catcatctga tagtaagaat ctccttgttc tccttctttt
    33061 gctatggaat cagtgaacag tttatcatag attttcaaac ccgtttcttt tgctacccgt
    33121 tccatgcttc ttgagtctac actggtttcc acgaataaaa ccggtacttc ttttgctctt
    33181 atctgatcaa tgatcgcttt catttgatca ggcgtacctt gactttctgt attgatctcc
    33241 catatataag ctgctggcaa atcatatgct cgtgaaaaat acttgaatgc cccttcactt
    33301 gttactaata attttttgtt ctcttcgata gaagcaaaac tttccttcgc ttgtgtatct
    33361 aattctttta gtttttccac atatgcttct gcattttttt tatagagtgt cgcattttct
    33421 gcatcttttt cagagaatat acgtgcgatt tcctccacat attttattcc gttagatagg
    33481 tctagccaag catggggatc tgcttttgta tgttcttcac cgctagttaa ataaagaggt
    33541 tctacatttt tgctaactgc aaaataatct tttccttctt cttttttagc cgtttccatc
    33601 aagttatcga accagctgtt acctgtttca agattcaaac cgttgtataa aataacatct
    33661 gcatcgcttg cctttttgat gtcttctggt agtacttcat attcatgcgg atctgttccg
    33721 actggtacga tactgtggat atctatttga tctgttccga cctcttttgc catgtccgcc
    33781 aagatcgaat tggtagccac gactgacaac ttttctttac ttccttcatc cgcttggtca
    33841 gaagcctgct ttccgcaacc ggctaatacc aagaaactaa ctgccaagat taaaaagagt
    33901 gatttttttg ttttcatcta tctgcttcct ttctgaaata aaattccttt tgttggagaa
    33961 aataaaaaag caaggataaa aaatgcggta catgctaaca cgatcgtagc tcctgatggc
    34021 caattgtaac tataactgaa aaacacaccg acaactgcac ttagcatacc gattcctgca
    34081 gctgtaatga tcattttcag taaatggttc gttagcaagt aagccgtggc ggctggtgtg
    34141 atcaacatgg caatcaccaa gattgttcct actgtctgta aactgactac agccactaat
    34201 gtcaaaaaga acatcaaaag ataatggatc agcgatgtgt tcagcccata agcttttgcc
    34261 attgtcggat cgaatgatgt gatcttcaat tctttataaa agaaaaagac aaatatcaag
    34321 acgattccac taacgagtgc tgtcagcaac aaatcgcttt ccctaacagc taagacattc
    34381 ccaaataaaa tatgatacaa atctgtcgca ctctgggcat atgaaatcaa gataatccct
    34441 aatgcaaaaa aggagctaaa gacaattcca atcgctgtat catttttcaa tcggctccgc
    34501 tgagtcacga aaccaatcac tgctgctgtt agcatcccaa aagcagtggc tccaaagatg
    34561 taactaaaac caaacatata agaaatggct acacctggaa gaactgcgtg agagatagcg
    34621 tctcccatca aagacatccc tcgtaaaata ataaaagaac cgatgactcc agaagcaagt
    34681 cctacaagca tggaagtgac caaagcgtat tgcaggaatt gataattcat taatccatca
    34741 ataaaggatt ggatcatttt ttctctcctc cttttactaa atgtctgatg atctcaccat
    34801 aggccaattg aaggttcttc gatgtaaaga cttcttgtac tggaccggaa gcgatcagct
    34861 gtttatttaa aagaataacc tcatcgaaat attcttctac tttatgaaga tcatgatgga
    34921 cgatcaaaat cgtttttcct gaattcttca attcctgcaa gatgtcaaag atctttcgtt
    34981 cacttaacgc atcaatccct acgaatggtt catctaaaaa gatccattct gctccttggg
    35041 ctagagctct tgcaataaaa actctctgaa gctgtccacc cgatagttcg ctgatctgtc
    35101 ttttggcata ttcttccatc cctacttttt tcaaagcttg tcttgcacgt tctttttctt
    35161 ttttcccagg tctttgtcca attcgtaaag atggatatgt tcctaaaagt acaacgccta
    35221 atacatcaat tggaaaggaa agatccaatt cacttcgctg ttccacgtag gctatttttt
    35281 tacggactga gtttaccgac tgtccagaaa aggtgacttt accagtagta gctggtatca
    35341 actctagcaa agatttcata aatgttgatt ttcctgcacc gtttggtccg ataataccgg
    35401 taattttttg tggatttatc gtcaagttga tagttttcaa aacagttttc ccttgataag
    35461 cggctgaaag gtcttgaatt acaatggccg tacgttccaa atcttacact cctttttagg
    35521 tttacctaac tttttattta aatatagttt aactaaaatg gaaagcattt tcaagtagaa
    35581 tgatgaaaaa gtttttttcg aattttcctc gcattttatg aaaattttct gtagaatcac
    35641 atcaagagtt caataaggct aacctaataa tgaaaaacat gtgttgaaac gtttgattat
    35701 tttttcttgt tgactcaaca acgaaaggag tttttgtatg gaagaagaaa agttaactcg
    35761 aagtcttagt gcccgtcata tccaaatgat cgcactaggt ggaacgatcg gtgtcggtct
    35821 gtttatgggt gcctcctcaa ccatacgttg gacagggccc tcagtgatgg gagcctatgc
    35881 gattgcagga ttctttttgt atttgattat gcgtgcttta ggcgaaatgc tttacgttga
    35941 tccttcgact ggttcttttg caaattacgc gagcgagtac atccatcctg tggctggcta
    36001 cttgaccgct tggagtaata tttttcaata tattgtcgta ggaataagtg aagtgattgc
    36061 tgtaggatct tatatgaatt attggtggcc tgatttgccg gcaattattc ccggaatcat
    36121 tgtcgtcttg tttcttatgc tggcaaatct tatttctgtc aaggcgtttg gcgaattaga
    36181 attttggttt tcgatcatca aagtcataac gatcattttg atgatcattg caggtcttgg
    36241 ggtaatctta tttggatttg gtaatcatgg tcaggcagta gggatttcaa atctttggaa
    36301 aaatggtgga ttcttcactg gaggcgtcaa aggatttttc tttgcattat cgatcgttgt
    36361 tgcttcctat caaggaatcg aactgatcgg aatgactgct ggtgaagcag agaatcctaa
    36421 aaagacgatc attgaagctg tccagtccac tatcggtcga atcttgattt tttatattgg
    36481 tgctattttt atcattgtga gtatctatcc gtggaatcaa ttgagtgaga ttggttcacc
    36541 ttttgtacaa actttttcta aagtcggcat tacatttgct gcaggactga ttaattttgt
    36601 cgtgattaca gcggcattat caggttgcaa ttcagggatt ttcagcgcaa gtcgtatggt
    36661 ctatacactt gccataaatg gaaagatgtc taaaaaattt ctgaaactat ctcgtcacgg
    36721 tgcgcctttt tatcctgtcg tagcaatctc aggtggaatc ttaattggtc tgatcttaaa
    36781 ttatcttcta ccaatgttca tcaaccagtc agaaaatatt ttcgttttcg tatacagctc
    36841 aagtattttg cctggtatgg ttccttgggt agtgattttg atcagcgaga ttcgcttcaa
    36901 aaagaagcac gcagataaaa tgcatgatca tccttttaaa atgccactat ctccttatac
    36961 gaattatgtg acacttgctt ttttagctat cgtattgatt ttcatgttta tcaatcctga
    37021 aacgagaatc tcattgatta ttggactcgt tttcttgctt tacatgacga tccattactt
    37081 tgtacgcgaa aaaagaaccg gaaaataaaa aaagaacgaa gcttcacctt agtggaagct
    37141 tcgttctttt ttattgcttt tatcaattca attttagcgt ctcaccggga gtcaggaaat
    37201 aattctccat gctcatccca ttcagcgaca agatcgtttc tactggtacg ccagttcgtt
    37261 cggctatttg atttggacct tctccttcca aaactacggt tgtcgcagcc tgtgaagtcg
    37321 cgctgttggt attagtttca gaggaatctg ttgtgtcttg tgttgttcga gcttgcattt
    37381 cagaagcttc taccgactct gctgtctcag aatcagaaga tgttgtgtct tgtgtgcttg
    37441 ttgactctgc ttgaagagag gcgctagtct gcaaataaat atcggaaatc ttagcgacat
    37501 gattcgtata attagccgat aattgattga tcaaagtatc taagtctaca ccgtctttat
    37561 tttctgctgc ctgatacgct gcacgtaatt ttctgatccc ttcgtcggag attgcttgaa
    37621 gctcacgtgc tttttggtta gcaactgctg ataaaccagc tattccctct tgatttccct
    37681 gaatttctgt ctggtattct tcaatcaatg caggcgtctt ttcttcgatc tttttcgaat
    37741 aatcatttac gattgcttga agttgctctg gcaaaccatt tgctgttttt tcttcagaag
    37801 tttgtgtctc ttttgtttta gttgatgact cacttgtttg tttcgtctct gttttctttg
    37861 tcgttgtttc cgttatggtc gtacttttcg cttctgacga attttttcca tgaaaatata
    37921 gacccgcaac tcctcctgct atgatcaaaa acacggcaac tgctgcccca atcatgatca
    37981 tccgcttacg agctgcctgt ttttgctgtc ttcttcgggt catccgaaca ttttctcccc
    38041 actcattttc atcatacatt tttttcacca ccaattttta tgtataatag cgttatcaaa
    38101 cgcttcatat tcttattgta gcaaataatt gtcgctgtgc acatttttat ctttgttatt
    38161 tataagaaaa tttcaccagt tgagatgatt ccaagattgt ccgtcgattt ctccttgcag
    38221 ctgcattgtt cgttcgtcta tctcagccat cagtttcttt atttcatata agagcgcacg
    38281 gtcttgatat tctgattttt cgcttaattc atctaattgt tggatcagcc aatcatttgt
    38341 cataatgaag ttcctttcat ttcttgcagt gtaaaggtca agtcttcgat ctgctctttt
    38401 gcctcagtca gtgcttctac gatatatgct tgtttttcag attcttcttc ctgcattttc
    38461 tgattttcct gtactctttg aataaaccag tcaatccgct gagtcagcga tggtttgcct
    38521 tccaatgaga gatactgctg ataagcaatt agcttctccc aacgtttttc ctgggataaa
    38581 tacataaatt gtgctaaaac gaatggcgga agataggtca tatggaattt atttgcgatt
    38641 cgctgatacg gcctcaaaaa ttcggaaatc gtatattctt cttttcctcc agcttggtat
    38701 tctttttccg ccacacctgt tgtcaatacg attcctagtt ctttcccttt taatacatgt
    38761 gctgctcgtt ccaagacatt atcttgccaa atttttaaat gagcaggaga actataccag
    38821 taaaacggaa actggaaaat gatccgatca tgctgggcca agagctgtag ttcagtctct
    38881 atatctattt ttccgtctgg ataacttgat tccaaatgat gccaagtagc tggtaaacca
    38941 gcagaagctt ctttgaaaaa ttgctgattt aaagattctt cgatcgttgg atggctaatg
    39001 atgataagtg tacgcatccc ttttcctcct ttttagtctt cgctttacag tgtagcataa
    39061 ccgccacaag tttcttttgc cgatgcttgt cattttaacc agactaatca ggttgtccaa
    39121 gaaactgtgg tttgtttttc caactgtttt ttcaaggtat ccaaagcaat attcttaaaa
    39181 tttttgtttt tcatacgttt acgatagttc gtagccgttt ctccggtact ctttttgaac
    39241 attcggctaa atacgactag actattgaac cctacttgat aggcaatact ttccacggat
    39301 tcatccgtca gagaaagaag aaacgcccct cgattgatcc gtaccccgat caaatattcc
    39361 ttgatactgg tacccatatg atttttgaat actcgcgaca aatggctccg attgaccgct
    39421 aaatgctggg cgatttcctc aacagacaat ttttcagcat aatgtgcctc gatgtatttg
    39481 actgtttcaa cagctaaaga ggaatgaacc ttttgtacac caccgctgac ctttccgcca
    39541 tcttttaaaa ggactgataa aaaacggtag gttaatgctg ttaactgtaa ttcattttcc
    39601 aaggtttctt ccgtatcttt catgcattct aaaataatgt cgatatattt ttgtatgtca
    39661 gaagagacca tcgtgtattg gtcttctctg aaaagcgact gccgaatgat ttcttctgca
    39721 gcactcccac caaatgaaag ccagcaatac atccacggat cttctccatc tgataagtaa
    39781 aacgtcgagt ctcctggacg aatcaagaac agatcacctt ttttcaattg gaattgttga
    39841 tttttcacat gataagatcc tttgccttcc atcacgacat gcaggatgta atcattgcga
    39901 atagcaggac caaaagaatg aaacggcatc gttttagaga aaccacaaaa atcaaaatac
    39961 aattcttttt ctacttgttg atgttccttt tttaaaaaaa tcgattgctc c
//
LOCUS       DACEXQ010000005        36695 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.005, whole genome shotgun sequence.
ACCESSION   DACEXQ010000005 DACEXQ010000000
VERSION     DACEXQ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.005"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..1255
                     /locus_tag="IXH04_000185"
     CDS             <1..1255
                     /locus_tag="IXH04_000185"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAQ1850824.1"
                     /translation="LNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVI
                     KKLNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKE
                     RRFFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEE
                     MNLRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKA
                     DFAFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFF
                     GTSIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEK
                     YAHLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKKLLEA
                     ISSLQTTPENEAWLQGALEVFKHEQ"
     gene            1245..2456
                     /locus_tag="IXH04_000186"
     CDS             1245..2456
                     /locus_tag="IXH04_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAQ1850825.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKVFEISKVTYGGGTPKTNTKEFWNGNIPWIQSSDLEINRLFNVSPKKKITNEAVKK
                     SAAKIIPPNSIAIVTRVGVGKLALMPFEYATSQDFLSLSELQIDSYFGIFSLYSMLQK
                     ELKNIQGTSIKGMTKSDLLEKKVTIPKKYEEQQKIGNFFKQLDDMIALHQRKLDLLKE
                     TKKGFLQKMFV"
     gene            2469..5618
                     /locus_tag="IXH04_000187"
     CDS             2469..5618
                     /locus_tag="IXH04_000187"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HAQ1850826.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKILMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKTGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLTGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(7553..8218)
                     /locus_tag="IXH04_000188"
     CDS             complement(7553..8218)
                     /locus_tag="IXH04_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1850827.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            8397..10379
                     /locus_tag="IXH04_000189"
     CDS             8397..10379
                     /locus_tag="IXH04_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAQ1850828.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERVISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            10383..11021
                     /locus_tag="IXH04_000190"
                     /pseudo
     CDS             10383..11021
                     /locus_tag="IXH04_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            11049..11477
                     /locus_tag="IXH04_000191"
     CDS             11049..11477
                     /locus_tag="IXH04_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1850829.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            11934..12845
                     /locus_tag="IXH04_000192"
     CDS             11934..12845
                     /locus_tag="IXH04_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1850830.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            13062..13499
                     /locus_tag="IXH04_000193"
     CDS             13062..13499
                     /locus_tag="IXH04_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1850831.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            13594..14478
                     /locus_tag="IXH04_000194"
     CDS             13594..14478
                     /locus_tag="IXH04_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAQ1850832.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            14702..17869
                     /locus_tag="IXH04_000195"
     CDS             14702..17869
                     /locus_tag="IXH04_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1850833.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            17889..18434
                     /locus_tag="IXH04_000196"
     CDS             17889..18434
                     /locus_tag="IXH04_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HAQ1850834.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            18462..18917
                     /locus_tag="IXH04_000197"
     CDS             18462..18917
                     /locus_tag="IXH04_000197"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HAQ1850835.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            18914..19672
                     /locus_tag="IXH04_000198"
     CDS             18914..19672
                     /locus_tag="IXH04_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ1850836.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(19758..20498)
                     /locus_tag="IXH04_000199"
     CDS             complement(19758..20498)
                     /locus_tag="IXH04_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1850837.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            20559..21812
                     /locus_tag="IXH04_000200"
     CDS             20559..21812
                     /locus_tag="IXH04_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ1850838.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            22134..22361
                     /locus_tag="IXH04_000201"
     CDS             22134..22361
                     /locus_tag="IXH04_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ1850839.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            22484..22810
                     /locus_tag="IXH04_000202"
     CDS             22484..22810
                     /locus_tag="IXH04_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ1850840.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            22878..23591
                     /locus_tag="IXH04_000203"
     CDS             22878..23591
                     /locus_tag="IXH04_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAQ1850841.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            23901..24752
                     /locus_tag="IXH04_000204"
     CDS             23901..24752
                     /locus_tag="IXH04_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1850842.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            24873..26738
                     /locus_tag="IXH04_000205"
     CDS             24873..26738
                     /locus_tag="IXH04_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1850843.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            26786..28195
                     /locus_tag="IXH04_000206"
     CDS             26786..28195
                     /locus_tag="IXH04_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAQ1850844.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            28303..29004
                     /locus_tag="IXH04_000207"
     CDS             28303..29004
                     /locus_tag="IXH04_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1850845.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            29163..29642
                     /locus_tag="IXH04_000208"
                     /pseudo
     CDS             29163..29642
                     /locus_tag="IXH04_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            30490..31065
                     /locus_tag="IXH04_000209"
     CDS             30490..31065
                     /locus_tag="IXH04_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAQ1850846.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            31065..31505
                     /locus_tag="IXH04_000210"
     CDS             31065..31505
                     /locus_tag="IXH04_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAQ1850847.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            31521..32222
                     /locus_tag="IXH04_000211"
     CDS             31521..32222
                     /locus_tag="IXH04_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ1850848.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(32710..33135)
                     /locus_tag="IXH04_000212"
     CDS             complement(32710..33135)
                     /locus_tag="IXH04_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1850849.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            33509..34480
                     /locus_tag="IXH04_000213"
     CDS             33509..34480
                     /locus_tag="IXH04_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1850850.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            34512..35909
                     /locus_tag="IXH04_000214"
     CDS             34512..35909
                     /locus_tag="IXH04_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1850851.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            36068..36691
                     /locus_tag="IXH04_000215"
     CDS             36068..36691
                     /locus_tag="IXH04_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1850852.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
BASE COUNT        12531 a         5971 c         7544 g        10649 t
ORIGIN      
        1 tctaaacaaa gcctttgtgc aattggcatc tacctacaat caatttaatg ggttatttga
       61 tgatgtggat ctccaatcaa aaaaactagg aacagatgag caacaacgaa atgtgacgat
      121 taccgaagtc atcaagaaat tgaatgatgt agatgtttta gaacacgatg gggacgtgat
      181 cggggatgcg tatgagtttc tgatcagtca atttgcttca gaagcaggga agaaagccgg
      241 agaattttac acacctcata tggtttctga tatgatggcg caaatcgtaa cgctcgatca
      301 aaaggaacga cgattcttta gtgtttttga tccaacgatg ggttcaggtt cattgatgtt
      361 aaacgtacgt aactacttaa cacacccaga taacgttaaa tatcatgggc aagaactaaa
      421 tacaacgaca tacaatctag caaaaatgaa cttgatttta catggtgtcg acgccgaaga
      481 aatgaatctc cgcaatgggg atacgttgaa taaggactgg ccaacagatg aaccttacac
      541 atttgatgca gtagtaatga acccacctta ttcggctaat tggtcagcag atacgacttt
      601 cttagatgat tctcggttta atcgttatgg gaaattagcg ccaaaatcca aagcagattt
      661 tgcgtttctt cttcatgggt tctatcattt gaaggaaaca ggtaccatgg ccatcgtctt
      721 accacacgga gttttattcc gtggcgctgc agaaggggtc atccgtcaaa aattactaga
      781 agatggcagt atttatgctg tgatcggtat gcctgcgaac ttgttctttg ggacatcgat
      841 tccaacgacg gtgattgtgt tgaagaagaa tcgtcaaact cgagatgtat tattcattga
      901 tgctagtcgt gaatttgtta aagggaaaaa tcaaaataaa ctatcggaag aaaacattca
      961 aaaaatcctc gaaacctacg ctgaaagaaa agatgtcgag aaatacgctc atttggcaac
     1021 ctttgatgaa atcaaagaaa atgactataa cttaaatatc ccacgctatg tagatacttt
     1081 cgaagaagaa gaaccaattg atatggttca tgtcggaaat gacatcaaga agatacgaca
     1141 agaacagcaa gtgctagaaa aaaaactttt agaagctatc tcttccctgc aaacaacacc
     1201 tgaaaatgaa gcgtggttac aaggtgcgct ggaggtgttc aaacatgagc aatgatacac
     1261 aaccagaaat ccgtttccca ggatttacag atgattggga acagaggaag ttgggggact
     1321 tcgttgtaga ttatgttgaa aagacaagtg tacaaaatca atttcctatg ctaacttcat
     1381 ctcaacaaaa agggatagtt ttgcaagaag actattttgc aaatcggcaa gtaactacag
     1441 aaaataatat tggttacttt gtactaccta gaggatattt cacgttccgt agtcgaagtg
     1501 ataatgatgt gtttgtgttt aaccgtaatg acattattga tagagggatt ataagctatt
     1561 tttatcctgt atttacttta aaatctgctg attccgactt cttcttaaga cgtattaaca
     1621 atggaatcca acgacaatta tctattcaag cagagggaac aggtcaacat gttctgtctc
     1681 taaaaaagtt taaaaatata gtagcaatgt ttcctagtga ggaagaacaa caaaaaatcg
     1741 gcaccttctt caaacaactt gacgacacga tcgctcttca tcagcgtaag ttagatttgc
     1801 tgaaggaaac gaaaaaagga ttcttacaaa aaatgttccc tgaaaacgga gcaaaagtgc
     1861 cagaaatccg ttttcctggt tttactgaag attgggaaga gcgtaaggtt tttgaaattt
     1921 caaaagttac ttatggtggt ggtactccta aaactaatac aaaagaattt tggaacggta
     1981 acattccatg gattcaaagt agcgatttag aaattaacag attatttaat gtttctccta
     2041 aaaagaaaat tacgaatgaa gctgttaaaa aatctgcagc aaaaattatt ccccctaact
     2101 caattgcgat agttacacga gtgggggttg gtaaattagc attaatgcct tttgagtatg
     2161 ctactagcca agatttttta tcactttcag agttacaaat agattcatat tttggaatct
     2221 tctctttata ttctatgctt caaaaagagc ttaagaatat acaaggaaca tctattaaag
     2281 gaatgactaa gtcagattta ttagagaaaa aagtgaccat tccaaaaaaa tatgaagaac
     2341 aacaaaaaat cggtaacttc tttaaacaac tcgacgacat gatcgctctt catcaacgta
     2401 agttagattt gctgaaggaa acgaaaaaag gcttcttaca aaagatgttt gtctaggagg
     2461 gaagaagaat gaaaattagt caccgtgagg aagcggaagt cgaagaacag ttgattcgtg
     2521 tactggggga aggacataat cagtggacgt atcgtcccga tctaaaatca gaagaagacc
     2581 tttgggtaaa tttacgtcaa aaaatcatct caaataatca agcagaactg aatgattttc
     2641 ctttgaccga taaagaattt gaaacgatca aaacggaatt attgctacga acgaaaaccc
     2701 cctttgatgc tgcgaaatgg ttaaaaggtg aaaatggaat ggctcggatc acgattgaac
     2761 gggaagatcc gcaattgggt tctgtctcgc tcattcttta ttccaatcaa gacattggtg
     2821 gaggaatctc tacttatgaa gtggtccatc aaattgcaaa acgtggaagt aacatagaag
     2881 cacgggatcg acgttttgac gtcaccttac tgatcaatgg attaccgatt gtccaaatcg
     2941 agctgaaaca agtaaccgca aaagatggtt tttatcaggc gtttaaccaa atcaaaaaat
     3001 atgcagaaga aggtatgttt cggaacaata tcttttcgac cttacagttg tttgtggttt
     3061 ccaatgaaca aaccactcgg tattttgcaa atgccttacc aaaggatttg catcctaagt
     3121 ttttatttag ttggcgaacg aaagataata aaaaggtaga aaacttatat gagttttgca
     3181 aacaagtgct aaatatcccg gatgctcatc gcttgattgc ggattatacg attgtcagtg
     3241 aagatcaaga caataaaatc ctcatggtgt tacatcctta tcaagtacat gcgattcaag
     3301 ccttgtttat tgctgcaaat aaacatcaat cagggtatgt gtggcatgcg actggttcag
     3361 gaaaaacgtt gactagtttt gtatctacta agcttttagc acgaaaaacg gggatcgatc
     3421 ggacgatcat gttagtggac cgcaaagatt tagataatca aaccaccaca gagtttacga
     3481 aatttgcttc ggagtttaat actgggattt cttctggtaa tgccaaagcg aatagcttga
     3541 tcgtgggaac aggaagtgcc aaagaattaa gtgaaaccct gttggcagat gccaatgcaa
     3601 atgttgtgat cattacaacc cgtcaaaagc tagatgcagc gttgaaatat gcaaagaaac
     3661 aagaagagaa aaaaggaacc aatcgcttcc aaaaattaac ggggcaacat atcgtgtttg
     3721 tagtagatga gtgtcatcga gcgttaagtg ctgaaaatat ggaagaaatc aaaaaaatgt
     3781 ttccgaaatc cacttggttt ggctttacag gtacaccgat tttccctgag aatcggaaac
     3841 aagccaaagg gcaattggca cgtacgaccc atgatcagta tggagaggtc ttgcatacct
     3901 ataccatcaa gaatgcatta gaagatggct cggttttagg atttcaagta gagcatgaaa
     3961 acaccgtgga gccgacttca ctagagaata aaatctatcg gaaattaaaa gaagtagaaa
     4021 catatgcaga gtattcatca gaacagatca atcgtatgat tgatcaaatg gaacctgtca
     4081 aaaaagaaag ttatttggac ccagctgttt ttgaagcaga tgaacatatc caaaaagtca
     4141 ttcataaaat gttccgtcca gataatgcct atacaaaatt tgattttcga aatggcaggc
     4201 caacgaaatc tgcaatctta accacgagtt ccattgacat ggccaaaaag tattatcggg
     4261 ccatcaaaga gatgacgaaa gagcctgatt ggttaacaaa agagtttccc aatcagctca
     4321 ttcgtgaagg acgaacgatg gaggatcctg attttccacg aatcgctatt acctattcat
     4381 tagaagaaaa tagccgagat tcaagtgccc aacaagaaga aatgcaaaaa attattgagg
     4441 aatataatca gtattatgat acttcctggt cattagcaga cattgagcgt tataacggag
     4501 acatcaataa tcgtttggca cgaaaaagag cggaattcaa acaatttggg aaacaaatcg
     4561 atttagcgat tgtagtggat cgtctattaa caggttttga cgcccctacg attcaaaccc
     4621 tatttgtgga tcgtaatcta gagtatgcag gattgatcca agctttttca cgtacgaatc
     4681 gtacgtatcc tgataagaca aaaggattga ttgtgacgtt tcgtaagcca gcgacaatgg
     4741 agaagaatgt cgaggatgcg acaaaactgt attcaaaagc aaaagaagaa tcaggtttga
     4801 tttatccaag ttatcaggaa tccaaaaaaa gattcaaaca agcatatggt aagttaaaag
     4861 aggtttcatc catggaatcc attgatgaac atacgccact tgaaatccgt atcgagtacg
     4921 tcaaagcatt tcaagaattg aatcgtgcgt atgaatcgtt agtgacttat gacgattaca
     4981 atgatgacat ggtgaaatct ccaactttga acaatcaaat tcaacttttg gaagaacaaa
     5041 ttggcgtgta cgagaccgtg aagggttcat taatagagga agaaccagaa aaagaaggag
     5101 aagatttttc aacgatcgaa ttttatagtg aaaataccac taagttgtat gatattgatt
     5161 caacttatat tgatcaatta ctaggaactt atgctgcgaa tagttcagat gcacgagaag
     5221 aaattgaaaa agctttggct aaattgaata aaacagaagg agtcaaagaa gtttaccgac
     5281 aaattttaaa tgcatttgat gtggggactt tagatagtaa tgaagatatc tttgtcatta
     5341 aacgtcgcta ttttacacaa gcgagtgatg atttgattca tgtcttttcc aatgagtggt
     5401 ttgtttctga gaaagaatta catgcttcta atctgcaata tctaccaggt gaagatccaa
     5461 ttcctaatat gaaagcaatt atcaacagta aggattacga aggatataaa gccaaacatc
     5521 cagaagcgaa gccatttaaa tacccacaag aaatgaagcg agcatggcgt aaaatgttag
     5581 atgatgagtt gattccactt gagaatgaat tgaggtaaac gagaagagtt aagaaaattg
     5641 tacgattcaa agatggtagt caagtggaag tgtagaaaat agggatagat gtcttttatt
     5701 tgttcacatt ttataacgaa gtatttaaga cagctattga ataatgtttc ctccaccttt
     5761 ttcattcatt taccccaccc cgttgttata tgaccgtgtt ttgagaggaa ccaataaaaa
     5821 gcataggcta actaggggtt cctttttctt ttgttttttt ggaatccgct taagtgaaat
     5881 gtatctgaaa tgaaacggag ataaagtgat tttttgctat aatgacaagg ttttagctgt
     5941 ttcagatacc atgtaaccca aaatagttta ttgagaaaat aggagagtat ttgatactaa
     6001 ctagtaggaa aaaatgagat aaaaattaaa tggaatcatt tcaggtaatc tcaaataaaa
     6061 aaatatacag tcctgtggca ctttacctat gggactgtat attttttact ctcagcgggc
     6121 ttgttttgac tccgtgtctg actccgtcgt attgatttta aatgatattc gatgaccatt
     6181 taacaggtta tttttgaaat ttttgtctct atatgactca gtttgataat tattgataga
     6241 tcaatttggg tctatatcga acaagggaat aatccaataa aaaatgattt agaatatcaa
     6301 aataacaaaa aagaggacta cctgtattcg tcctcttttt tgaccagatt ttgaccagtc
     6361 taatttacat tctaatatat tcatatgagc taattttttg attaaatggt aagtttttcc
     6421 ttgtttgttt tagtttaaac tagtgtgtaa tttaaaaatc tatatggaag ggtctgtggg
     6481 gtaatactta aataaaggct aggaacctga taacatcaag gttcctagcc tttttataat
     6541 aaaatgaagc gaaaataatt tcttttaaag agccgagatt atcagctata taagagtgaa
     6601 tttgatgcaa aaaacagagc cgaaacagtc ttggtgttcg tagctctgct tatatgtttc
     6661 gaatttaaga caaagagttg gcaaaagatg ttgataagga agataagata actgtgttat
     6721 ataaactagg taactaactg taacaagcta attttaagag attcttatta gaagacgttt
     6781 aggaatataa ctaagaaaat gattatacag gtaattgtca tttcaataat gttttctaat
     6841 tttcgcgtct ttgttttaag aaaaaggcca gaatcagttg gaaaaaagtt cgtacgatag
     6901 tgaagagggg caaaatttcc tactaatatt cccactaaca agagataaaa cggccaggtt
     6961 ggtgtgattc ctctataaaa aaatgcccat gctaccgaaa tcccatagat taacaactga
     7021 acaattttta caatgaaaaa attgttctgc cattactcaa agtctcttcc tccttgtatc
     7081 gtttatttgt ttcattttat cattatgata ggcgcttgaa caatccattt tgtattttat
     7141 tgagtactct tggataaact accagcgatt atcatattta tttatgttac tgtaagccat
     7201 catataaaag taaatgagca gatcatagaa atataaaaaa gaactaaaat tgatctaaat
     7261 tatgtgcagc ttgactacgt accaatctat attacttact agattggtcg ttaatctatt
     7321 cggatcgaat gagcaaaata tttttgtgaa tgtccatcct tgattatgat tccaaaaaga
     7381 atcattggga ggctgtggcg taaatattat actataagcc agaaatacct atcattctga
     7441 acttttttat ttaggaactt tcatctcaag gctttttcaa ttaatttgta cccctgtttt
     7501 ttggataaga tcatgtgcat tgattttttc tatctctacg ttcatctcct acttaatgcc
     7561 tatctggctc ctgagggctt ctttttaagg cgtcgtcata gtcttggctg aggatttctt
     7621 ggtaatctga gggaatggct tcccaagtga gcaggcgaac gttcatgacg tgttttcctt
     7681 tgtgtttgat tacagcatgg tttttctctt tttctacctc tccagcgtga aaagacagcc
     7741 atgtaccatg gccaaagata tcattacata cccaacgttc gatggaattg tccacgtatc
     7801 ctacagggag tgtggtatag ccgatttgtg gtttcaaaaa aggattgaat cgatcgatcg
     7861 tttcattttt gtattgataa ttgataagaa aaagatagac caaacaaaag acgagcagaa
     7921 agaaagatag tgtctttttg agtgagaagg gttgttttga tgaataagat ttcttgttaa
     7981 caagaggttc atctggcaat gttggttcta ttgattcggt agctgattca atagaaggaa
     8041 aatcaatcgg cagacctaaa gtatgatgta aaagtgacag catttcaagg tctggcattg
     8101 tacgagaatt ttcccaacta gaaatcgttt tatcggaaac accaattttt tcgcctaatt
     8161 ctttttgcgt aagcttacaa tttctgcggt aagttcgtat tttttctccg gtgttcatct
     8221 tcttctcctt tcgattctac agtatataga tttattctac atgacctata cacttgatag
     8281 atacgttttt ttgatgaaaa acggtatgat tttatcaaag aatcatttaa gtaagggaga
     8341 gtggtgataa tcaaaaaaag ttcacagttt tactcacaat catagtgacc ggtatcatga
     8401 gtggttttgc cttggttagt ataaataaac aggtactcta tcggacaaat gaatttttcc
     8461 atcaaacaga tacttatact ccgattcgat tgccacaaca ttttattggg caagaagaat
     8521 atgagcagct aatcatggta tttgaaaatg tctcatcaga aactgagctt tcttatatga
     8581 aaaaaacagt aaatgaatcc aatgaagtag atacatcttg gaacttcaag atacagccaa
     8641 agcttgaaat gacattgtat tatcaagggg atgtcaaatc ttctcacgag aaggtaccct
     8701 attctaatac ctcctttcgc gtaacgtcca ttgaagaatc gatcaaaata aaagattttg
     8761 aaggtgaatt tttcttgatg acgagagaaa aagaagccta tgaacgggtc atttcactat
     8821 tcactgatcg attcaatcaa gcatttcaca ctgattatgc cactaatatt tttgaagact
     8881 ttgaaaatag ctattcgtat tcggttattt caaatacaga cgtatataat tatcgagtgt
     8941 atttaactat tggattgatt cttttattcc ttttgttatc atcctttttc ttattgatga
     9001 ataaagaaat cagtttattg ttgaataatg acttttcatt acatcaagta ttgtggtgtt
     9061 tattggggaa aaagaaaatc attattacat tcataattgc aggaatcatt ggtggtttta
     9121 tttttatgta ttataaggag caatttcttt atttgttcca gatgtacgtt ttcttgttca
     9181 gcggaatata tggtatctct ttgatttctc ttttattagt tgaaaaaatc gcttcgttaa
     9241 gagaagcgtt caactatatc caatacattc tttttacatt tggattgttg ttttctcttc
     9301 ttttgaccgt tgccaatgtc gaattagcaa ctatcgtttt ttcttcttca cagttagctg
     9361 tttcccatga accaccaaaa gaaatcgctg gtgaggacta tcatgtattt tatccagtaa
     9421 ccatcgggaa aaaccacgtg gattttattt attccaatcg ttttgcatca gcagcagatc
     9481 aggaattata tgaaactttg aataaaaacg gaagtatatt agtcaatgta tcagattact
     9541 tgaatgaaga aaatgaacag tttggcagag ggataaaaat aaatctgaat tatcttaaga
     9601 aatttccttt gattgatgtc tctggaaggt tgatccaaat aaccgaaaaa gaaacgcacc
     9661 ctgttatatt gatccctgaa cgatatagac atacagattt aaaaaatgac ctggcgcaaa
     9721 taacagaata ttatcaagaa atcagtgaaa aagaaccgaa atttctattt ataaaaaaca
     9781 atcaacccat ttatacattt attccttcta tcccctggat cgaacattat ccggttgttg
     9841 aaatactgac attgaaaaac agtatgtatt tggaaagaaa tattctcagc ggagaaatct
     9901 atccaccgtt aaagatcaag ataggctctt tgtcgaaaga aaggttattt gagctgattg
     9961 aagaaagtca gttacgtgat aacttgcagt tatcatttcc gtatactcag actgaagaga
    10021 tagctgtcaa agtgttaagc agatcatttc attacttgat acagatattt ttactcactt
    10081 tcttttcttt cttcttgttg agctatgtca tgttatgcat atattttgtc tacaattgtc
    10141 ggaaaatcgc tatctttcgt tcaagtggtt attcgctctt tgaaacgtat aaagatttct
    10201 tcatgatgaa cctcatcaaa tggggaacga caagtgtaat cttccttttt cttatagaaa
    10261 gagagcctaa atatcttttt aatatttttt tctttagctt cattgatttt attctttcag
    10321 ttgtttttat tttgtctaca gaaaagaaaa gtcagttatt gctgatgaat ggaggttaac
    10381 aattggagcc gttgatagtg ataaaaggtg cgaaaaaaat cattcgaaac aaaacgattc
    10441 ttcatttgga tcgtttcact gcggatgctg gggatcttgt gactgttgtt ggagaaagtg
    10501 gagcaggtaa aagtacgtta ttgaatatat taggattgaa tgatcagttc agttcaggac
    10561 aattctattt gttcgatcaa gagacgaaac aattaactgg gagagagaag cgcttgataa
    10621 aaagaaatac catatctttt ctttttcaag attttgggtt gattgaatat gaaacaatcg
    10681 attttaattt agatattgga ttgaagtttt cgaatttaaa tgtttcagat aagcgaacag
    10741 caaagtatga ggcattgaaa aaagtaaata taaacaagaa gctaagtacg cctatctaac
    10801 cattatctgg tggtgagaag caaagaatcg cctgtgcgag agtcttattg aagaatagca
    10861 aaatcatttt agccgatgaa ccgactgggt cattggataa aagaaataag gataaagtag
    10921 ttgaaatctt atcgcaacaa gcttcccaag gtacgttagt tatcgtagtc acgcatgatc
    10981 catatctgac caaaatagga aacaagaaga tcatgcttta aaaatcaaaa aggaaaggat
    11041 gtcgttttat gaaaaaagca gttattattt gtgcactagg gataggtttt gttggaggaa
    11101 acgctatgtc atctgaagtg gaagcaaatt cagttcacgg tgaaatctat tacaaatctt
    11161 taaagccagg agagattcat tttgatgcta aaggtaatat tattaagtca aatttaccag
    11221 tagaccggga aatttctaga acagcaatta atcatagctc tggaagatgg gtttattata
    11281 gtgatacaac ttgtacgaga aaaacaggtc attctaacca ctatagtcgt gtctataatc
    11341 gtcacggtgc aaaagcgaaa gttggaaact ctattcgatc agcaaatgca acaaaaggag
    11401 tatgggccta tgcaagtgcg acaggaaata gaactgacac attctcatgt tggtacaatc
    11461 caaccggttg gtattaagta tccaaaagtt tatataaaat agaaaaaaca tataatgaaa
    11521 cagcgttaaa agttattttt ggtagtttta atatattcat ttttagttgt ataaaaatat
    11581 atcataataa aaacaacaaa aaaatgtctg gaaaatcgaa cataaaagtg tctgatttga
    11641 atatcccagc agcggaagaa gaaaaacaaa atcgataaca tttgttctaa gaattagtta
    11701 cgttaaatac ggaacgcaag atagacttga aaaacggtaa caaaagaagt agtatattaa
    11761 ggtgtgatgt gctacttgac atttattctg ctgagcgaac tttctggatt gtgtagcctt
    11821 atatctattc aggacgtatc gagcagatta gtaaaggtgt tcttgatgat tttcatcttg
    11881 aacacctttg tttttagcaa ttcgcaaaaa acgaaaagta ggagaattga taaatgaaaa
    11941 aaagattttt agcacttgcc atcgtactag gcactggctt attgtcaggc tgtacgaatg
    12001 ccggtgaaaa aacagccgtt agttataaag gcggtacaat cagcgaacaa gaagtaatgg
    12061 atagcttgaa aaaaatgcaa ggagcagatt ccgctgtgca acagttgatc gtttatcaag
    12121 tatttgaaga taaatatgga gatgacgtat caaccaaaga aatcgattct caatatgatc
    12181 aaacaaagaa acaattgggt gattcatttg attcacaatt gaaatctgca ggttacacag
    12241 aacaaacatt caaagacagt atcaaacaaa gtctagcctt tcaagaaggt ctaaagaaac
    12301 atatcaaatt gaccgatgaa gatttaaaaa cggcttggga atctttccat ccagaagtgg
    12361 aagctcaaat tatccaagta gcaagtgaag atgatgcaaa agatgtgaag aaagctgctg
    12421 ataagggaga cgatttctct aaattagcca aagacaagtc aactgatact acgacaaaag
    12481 aagatggcgg aaaagtgaaa tttgattcca ctactacaac ggttccggca gaagtcaaag
    12541 aagcagcctt taagttgaaa gatggtcaag tttctgatgt gatcacttct accaatgctt
    12601 caacttatac tacggaatat tatgtggtta aaatggtgaa aaaccaaaac aaaggcaacg
    12661 acatggataa atacaaaaaa gaattgaaag aaattgcaac agacacaaaa ttaagtgaca
    12721 gtaccttcca gaataaagta attggcgaag tattgaaaga cgctaatgta aaaatcaaag
    12781 acaaagactt tgaaaatgtt ttgagtacat tcacttcaga ttctagtaca gcaagtagta
    12841 aataagcggt ataaataaaa aatatgaact atttaaatca ataaaactgt tcatctaaat
    12901 ctttttatta gaactaaatc aacagtagtt taattactac ttgatttagt tctttttatt
    12961 ttaaaaattt tttttactta gaatccagcg accctagata tttttctgta taatcttaaa
    13021 taaagaaaac aatatcaaaa aatgcacgta gaagtagata aatggagtgg ttttgcaaat
    13081 ttcttttatt tctagaagtt attcacgtat acggatttgt ttttctcaca aataaaaagg
    13141 caggaagtac aatggatcat ttgtatataa aatatattca tcaattttcc gttttggtgt
    13201 taggcacact aattatagcg gggattgtcg catttattat tcaaggccgt gacatattaa
    13261 cagtacatca agagtttttc agttatgcat ttggtattcc tctgatcgct ataaggtctg
    13321 ataacaaaaa gtggaagaaa cgctattaca ttctcttgct atttttgtgt ttttatctgc
    13381 cacctaagat ttttacttta ttaggtatac aatgcctatc attttctttt atttcctata
    13441 tcattgtttt aattttcctc agtttcatag tatggatgtt ttatcttgtc aaaaaataaa
    13501 tgaatgtttc atgaacagta cttcatcaca gcctaatttt tttctttatg ctatcgttat
    13561 attaaagaaa tcaatcagaa gggaaagaaa gtgatgaaaa aagttttatt agtggtaaac
    13621 cccagttcag gcggtgaaca agcaaaggaa tttgagcaat tagcgatagc aaaattggaa
    13681 tcggtgtttg atgaagtggt cgtcttgcat acaaaaaaag caggggatgc aaaaaacttt
    13741 actcgcgaag cggctacgga agggtatcat agtgtgtttg tcatgggcgg agatggaaca
    13801 gtcaacgaag gaatcagtgg aatcgctgaa caagaacatc ggccaaattt cggatttttc
    13861 ccattgggta cggtgaatga cttggctcga gcattaggga taccgctaga acctgaggaa
    13921 gcgatcaatc atttttcgat tgaatcagtc aagccattgg atattggaaa aataaatgac
    13981 gactatttta tgaatgtcgt cgcaattggc tctatccctg aatcaatcaa tgatgtagat
    14041 gcggagaaga agacgaaatt tggaaaacta gcttatttca tgtcaggaat caaacagtta
    14101 gcttccacac aatcctactc gtttcacgta gaagttgatg gaaagaagga agaaattgag
    14161 agcagtacct tgctgatagg actaactaac tcagtcggcg gttttgaaac attacttcca
    14221 aatgcaaaag tggatgatgg taagctccat ttggtttatt taaaagattc ttctttgatc
    14281 gatacgctta aaacattgcc agacctatta aaaggagtag atgaatctac gaaaaatcta
    14341 gcctacctta cttgtgagca attagttgtg tcattagctg atgaggaaga attgacgacg
    14401 aatgttgatg gagatgaagg ggataaatta cctgtgacaa tcaaagtatt gccctcacat
    14461 ttacaggttt attgttaaaa tggaggttgt gaccgaggag ttggcgggag aacaccgctt
    14521 gttcgggata agggactgtg acaagacttt tgtcacagcc ttttttttgt acaaaaaaga
    14581 aatttacacc taatagaaaa atttttccat aaaaatacat attttaaata tgaaagtaca
    14641 attggaaata gattaaaacg ataaaataaa attgtcaaaa cgtcagagag gatggtggag
    14701 aatgaatcaa tgtgtaaaaa agctgattgc gtcgattttg tttgtcttcc ctctcttgat
    14761 ggtgacatcc gtttttgccg atgtagagaa aacaaacgaa tcaacgcaat cagagaatca
    14821 aagttttgac aagactactt tttcagaaga atcgattact tctgaaacta cagaatctca
    14881 aagtgaggct tctactaata aaaggcaaga agaaaataag acaagtgaga cctcagaagt
    14941 agctgaacaa acgtctgata gcagtgaagc ggcactggca agagctgttt cagcggatgg
    15001 gtacagcgaa gctgccacaa cagaagaact ggcgcagctt ctcgcagacg aatcagtaac
    15061 taagatccga ctgattcagc ctttgacttt agacagggaa ttagagatca aacgagatat
    15121 cgttattgat tttggtggat tcgctcacaa tttcggcaca caccatattt atatcaatga
    15181 gatacctcag tcgatcgagt tccagaattt taaaggaaca gctgcacatc caggtggatt
    15241 gacaccaaat atcgatggaa atgccattat cattgcttat atgaccgatt tttggggaag
    15301 cagatatcat tttacgggag aaataaaatt taccgggaca cttgatttgt atgatgggtc
    15361 caaattagga ctgatttacg ctcctcgtgc aactgtcaca ttagatggtg tttccggtgt
    15421 gttggatgtc caaccagtga aggaaggact gaatgccgct ccgggtaaag cctatttttg
    15481 tagaagctat caattgaatg tgatcaatgg tagtcaactt tatggaccgt atctagggaa
    15541 attctacggt tttctagatt caggagatga gactggtagt ggaaaaacag cgccaggaat
    15601 ccatatctta agcggttcaa aggtctctct tgattatgat cgggcagatg gcgttagcga
    15661 tgaaggagag gcagtcgata cgctacctgg taatgtcatg tttgaagtca gtggttcggg
    15721 aagcgaattt tcggtaaata caaagatcaa tattaccaat gacaacaatc gcggaatcat
    15781 ccaaatgaga ggttcaggat cgaaagtcac tgtatcgaat ggtgggaaaa tcaccatcaa
    15841 tacagcaact acgggcggat ttcgcttgca aggagattct agtcaaatcc atgtatcaag
    15901 tggtggacaa atctcggtca aaatggctgg agatgacgat caacccggga acaatgggat
    15961 gcgttttgta ggaaaaggcc tttctttgat cgtagaagga gccggatcag ttatcgacat
    16021 cgataagcag agtgggcgtt ccagcgcagt tcgttttgag aatggtacgc aaaagctgac
    16081 agtaaccaat ggaggctcat tgagggtaag aaatacaggt gatggcaaat cttacgtgaa
    16141 cagactcaat caaggaaatc aagcgatcca attttatgac gcttctggtt ttctagaaag
    16201 tccaggttca gctgatttta cagtcactgg agaaaaatca aatatcgata tacgcgcaga
    16261 ttccggagca actgtagata caactggaga tattgattta aattttacgg caggtgaaaa
    16321 aacttattta gtcatggtcg ggaaaacagg ggaagataag accggcatca tcagtgggga
    16381 tgtgttgaat gtggacttag aatctcctac ctattttgat ttccaaaaca aacggattgg
    16441 aagcgagacc aatggcatag gcggctgggt attccaagga aatggagatt cttctttaaa
    16501 gatcgcccat tcagaaatcg ctttatggcg aaaaacaggc gcaacattcg ataactttga
    16561 aaaagacccg gattattttt ctgatatgac agacttatcg atcaatggga ccgacttagg
    16621 caatttcgta tcaagcccta gtgcaggttt aacaaatgaa tttgctcaga caggtgccgg
    16681 aatgaaaaat tataatcgaa tcagtgccaa taatcagctc ccgatgattg aatccttaag
    16741 agtgccaacg aatgcagata aaaaaatcta tgggcatgtc gttgttcccg aaggtgtcga
    16801 ggcaaagcca agagatgctt ggacaggcga agtagaagtg actttacgta ttacttacgc
    16861 agatacgacg aaatcatctg tagaaattac tgctctcacg caaggacaaa cagatgaaaa
    16921 tgaaggggca ggatataacc catggggaga aggcgaacaa ggcggcttgt tcgaagttca
    16981 agtacccaat gatgagagat taaaaacgga ggatcaagtg caagttgttg ctgctaaaaa
    17041 agtaagaggt ggccaagaag tgacagaact ggactctgcc aaaacgacag tcgatgtgat
    17101 tcctcctgaa cctgctaatt taagtaccac attgatcaat gcagcttctc gcacgatcac
    17161 aggtacaggt gaagcaggag ccttggtttc attgaaaaaa aatcaagctg ttttgtcaga
    17221 aacaactatt gatgatacag gggaatttgt tctttctatc ccagaaaatc aactttttgc
    17281 aggagatgta ttagaaattg tattaaacga ccaagcagga gaagctactg ctaagggtgt
    17341 gcagaacaag ccaagtacca acgatgaaat tgggaatcac aatccaagtg atacgccagt
    17401 tgtttatcat gatgccccgc cttttgcacc tgctacgaag atcgtagtag aaggtggtct
    17461 ctcatttctt gccccttcca aactcgattt tggtcagatc aaagcaactg gtttgacaca
    17521 agaagcattt ggccaaatgg atatacagga aaaattatgg atttatgatc agcgagaaac
    17581 gaaaaatccg tggatcttaa cgataaaatt atctcagcct ttcacaaatg atgcaggatt
    17641 ttctatgcag gatgtcctat atttcaagaa aggagaggag tatcaattag tgaatgaaga
    17701 agaactggaa attgtcagag gacaaaattc aacgaatgtg caagcagatt attcagaata
    17761 tttaaataac ggtcgggcgt ttaaacttgt cttaaccaaa gaaaatcaga tagctggtaa
    17821 ttactcagca gagataacat ggacattagc agatgcgccg acagaataaa gatcaaggag
    17881 gatgattgat gaatcgaata tggaataaaa gtcatcgaaa caactgctct ttacttatga
    17941 tccttctcct tggattcatc ctttggcaga cacctgtcca tgcctccgaa ttcaattttg
    18001 cggtagattt tgaattaccg gctaatcaga ttgataagaa caaaacatat ataaatgtaa
    18061 ggatggagcc tgggaaaaca caggaaatcc cctacacggt ctttaaccga acagataaat
    18121 cagtaacagt tgagacattg atccactccg ctacgacgaa ttcaaacggt gtgatcgaat
    18181 atggagaatc aaaaagtaaa tttgatgaaa cattaagtta tgatatgaaa aacttagcaa
    18241 aaacagtggg agaaataact gttcctcctc aaagtgcggt cactgagaca ctttcactga
    18301 aagcgccaga aaaagagctg gctggtgtgg tcgcaggagg gatcacgttt caagaaaaaa
    18361 aagagaacgt ttctaacaaa agtacagttg gtgtcgtcaa tcagttttct caagtgaaag
    18421 caatcttgat ttgaaatagc gaaaaagagt cttccccaga tttgaaatta aaaaaagctt
    18481 atgcggatca aagcgatgga agaaatgtga ttaaggcgaa tcttcaaaat ccaaatgctt
    18541 cttatatgtt cggcttgact acatcggttg aaatctacaa aaaagaaaca aaagagcctt
    18601 acctttcaat aaaaaaagaa aaaatagatg tagcacccaa tacaaatttt gatttacttg
    18661 tcccattaga aggaaaaaaa ttagctccgg gacattatca agcggttgtg aagggtgctt
    18721 ggaaagaaaa acgctggaat ctccataccg attttgaaat aactgatgat gcagcggacc
    18781 aattgaatga aaaggatatt gatttaaaaa atcagccaga aacatttgac tggcgatggc
    18841 tgatacttgt tggagtcatt cttattggct tatttgctgg attatttatt ttaaaagaaa
    18901 gaaggaaaaa gaaatgaaaa aaacaattgc attaagtacc gttctagctg gaagtctatt
    18961 attatgtaca caaagtgttt ttgctgagaa taagagctca gccactacag aaggaacgat
    19021 ttctgtgacc aaaccagatg ctgcaaatcc tgaagacgtt gttccagttc ctcctgttga
    19081 tccaaatgat cctgatactc cttttacacc tgacgaacca aatcagggaa catccggatt
    19141 actgacgatt gaccaagctc cagatcttga ttttggaaca atcaagttag gtacagcaaa
    19201 aacagtatat gctgcattac aaagcggaac ggacagtact ggagcacaaa cacaagtaca
    19261 gaatttcgta caagtaacgg ataaaagcgg aaattatgcc ggttggaaat tatcagtcaa
    19321 acgaacagaa tttattggta caacatctga tacgaataaa ctaactggtt caaaggtgaa
    19381 attcaaaaac gccgtgattc gaaatagcgc aactaatagc agtggaatgc catctaccgt
    19441 ttctggaact ggcgctgaag gaatcgaaat cccagcagat caggcagtag atctagtaac
    19501 tgcaggagca aatgaaggga ttggcacatg gatttattca ttaggagaga atgtggaaca
    19561 agggagacaa tcagtggaat tatttgttcc tgtatccaat tatgctgtag acaactacgt
    19621 ttcaacattc acatggtcgt taacagatgc tcctacagaa gaggaagcgt aaaatcatat
    19681 caacgatttc agggagtgga gccgattcaa ggcccactcc ctgaaatctg ctatgcttac
    19741 gtctcagctc ttattcatca actcgaagca gttttttgga agtcatggaa acgttccttt
    19801 gtcttttcaa agatctttcg accaacgagt aaggagtcac cgttgaaaaa ttctaacaca
    19861 taatgagcag aatcaatttt ctgcaattgc gataagttga tacagtaaga ttttaaaagt
    19921 aaaaagtaag catacttttc taactctgtc ttcaattgtt ttaaagaagt attcactaat
    19981 ttatcttctg tagttgtttt gaccataact tggtttttca aacctttgat actttcaata
    20041 tataaaattt cccgaggata caggacgaga cttccacctt tttgatcaat aaagatttta
    20101 tcgacattta cgatctgctg ttctttccaa ttgatctgtt ctagcacttg acgaatttgt
    20161 tcagataaat tcctgtcttt tctgatatat ccaatcggaa ggattttccg attgatgatc
    20221 tctactgcaa aattcgtata agcggtgaaa aaaacaatat tactatgtgc atttttttta
    20281 cgtatttgtt cggcgacatc aatacctgtg agtgattgct tcaagtcgat atcgataaag
    20341 aaaacatcgt gattatcaat agtgcccaac tggatcaact gattgatttg agagatagaa
    20401 gtgagctgca cgatattctc gatatgatga ttgaccaatt ctctgatgat ccattcgctg
    20461 tgaaactggt tatcttctat gataaaatac tttttcatat gcaggctcct attaaaaaaa
    20521 tatgtgtaca ttttgaaaca aaacaatgga ggaataagat ggtcgcaaca gtgacacttt
    20581 atttgacctt tactttgatt ttagatttag gtatctattt attcttttcg aaacaactgc
    20641 tagtaaaaaa cagctttggt tgtatagctt tgatggtggt gacactttta cttcctatcc
    20701 ctaaagtgag ctttccttac ctagttggct tattattgat gtttcttttt tattttacta
    20761 cgaaaaacct gtttatcagc tgtttgatca gtaatctttt ttttgctatc actggactca
    20821 gcttttttct ggtttttgat ctgatggatt atttaggaaa gagacatctt ggaacagcaa
    20881 gtttttttgc actacttatc gcatttattt tctatctatt agtaaaaagt atagacaaac
    20941 agacgagatt tctcaacttg ctttttactt atccaactga acactatttt ggaggtattt
    21001 gtattttatt aagctggggc gtgatttact tggtattggg aagtgttcac ttccgctcat
    21061 ctaattatct gcttctatct cttttgtcga tagtcatcag ttttttttct ttgttttttt
    21121 ccattatgtt gatcagtttc caagtaaaag aaagacaaca tcaggaatcg cttcaactgt
    21181 atcaaataaa tgaagaatat taccatcatc tagaggaatt caaacatgat tataaaagtc
    21241 tactgttttc attaaaaatt actcttcagg aaaataaaaa agaaacgttg gaatttttag
    21301 aaatattaga agatcattct gcagctattt ttgaaaaacc gcaaatacaa cagctaacaa
    21361 atattgtatc cccagctgtc aggggtgtct ttttaaaatt tctagaaaaa gcagaatcgg
    21421 agaaaatccc gatccaagta gttattcctg tcgaagtgaa aacaatcccg attgatctgg
    21481 ttgtttttat tcgctgttta tctattttta tttctaacgc attcgatcat cgtgtagccg
    21541 atcagagtcc gattacgatc aatctgctgg aagaaaaaga gggatcacgt tttagtatag
    21601 cgaacaaggc aaatccaacg aatttgacgc tatcggagat ggtcaaaaga ggaaaaacct
    21661 ctaaaaaaaa tggcgggtta ggtctataca gtgcgaaaaa aatgttggat gtttacgaaa
    21721 atgcagaact aaaaatcgaa ttttcaaaac agaatcacta tttttttgta gaaatgtatc
    21781 tggcatctag aaagagcaaa tcagcctact gacttactgt caaaggataa gtttgaggaa
    21841 agtctacacg gagatagaga caacaacaca gcatattcaa aagaaacaga agcgtgatga
    21901 ccgttgtcac ccttctgttt cttttgctat gtagttaaac aaagtagttg acacttccgc
    21961 tacttagtat tagtacgaaa gtatacttca tgatactaag tagataagaa gtgaaaagcg
    22021 gaacccacga tcgtcaaaat gctaactgct ttgcttacac cagataaagg gaagagcaat
    22081 atcgcttatt ttaggcgtgg agaagcaagc aaatccctac gatcacaaaa aaaatgctga
    22141 cccagcagtt aagagagtta gaacaagatg aaagtatcca tcgaaaagcc tataaccaag
    22201 tcccgcctaa agtagagtac tcgctcaccg aagaaggaaa atcattaaga gagattctag
    22261 tagcgttgtc tgtttgggaa gaagaacgta tcgaaaaaca aaaagcctca agggaagaag
    22321 acacgctact gcatacacat ggtggctacc taaattattg attttctatg acatttattt
    22381 taaataaaag aaaacaggag tttttttaaa agcatcatct attttttatg taaaaaaatg
    22441 gtatgattaa tcaagtaaga agtgattgga agaaggcaag caaatgaata gtatggaaaa
    22501 agtcaaccag tttagagatg aacgtaattg gcgacaattt cataacgaaa aagatttggc
    22561 tatctctatt tctttggaag catctgaatt gttagaactt tttcaatgga aaacgccgga
    22621 agaagtaaaa gaaacacaat tagaacgaat caaagaagaa ctggcagatg tattgatcta
    22681 ttcatacatg atggcagaca accttcacct ggatctagat gagatcattg aagaaaaatt
    22741 gatcaaaaat aatttgaaat atcctgtaga aaaaagcgca ggtgtacgaa aaaaatacac
    22801 agagttgtag aaatgtgtca ttcggctgtt ggaaagagca aaagaaaaac agtaaaatga
    22861 gtctggaggg agagaaaatg aaaaaaaaag gaaaagcaaa aagatggcta gtcaatatcc
    22921 tcttgttcct cttgttatta gtaggactag ctttgatttt caatgaacag atcaaggatt
    22981 attttgtcag agagacagga gataaatatg ctatagcgaa tgtaacaaaa gaagatctga
    23041 aaaagaataa cgataaagat gtcagttttg actttgatgc ggtagaacca atgacgacag
    23101 aaggggtcat gaggtcgcaa atgagaggaa cagatctacc agtgattgct agtatcgcag
    23161 tcccttcagt ttcaatcaat ttaccggttt tcaaaggctt ggataatacg tcactacttt
    23221 acggagcagg aacattatct ccagatcaag aaatgggaaa aggaaattat gccttagcaa
    23281 gccatcgtgc aacgaaccct gaattgctgt ttacaccgct agaaaattta gagatgggtg
    23341 ccaagatcta tttgacagat ttagaaaatg tgtatacata caaaacattt tttaaagaaa
    23401 aagttgctcc tacagatacc caattactga atgaagtcga gggaaaagaa attgtcacac
    23461 taattacttg tggcgacatg gacgccgtca cgcgactagt tgtacaagga gaactggaaa
    23521 gtgttacatc gataaaagat gcgacagatg atatgagatc tgctttcaat ctagagacaa
    23581 aaacatttta ggatactggg acataagtcg ttacagatta aaaacagcca aaggatatga
    23641 atcgataagg aaaaaccaat ttgctgaaaa aaagagaaac gcaaagaggt gcatctctga
    23701 tttggttgtt ttctagatag acttgtgttc cagttttttt attttttata tgaaaaggct
    23761 tgcataaata aaaattcgat ggtatgatga ttttgaataa gaaatggatt gttactgtac
    23821 ggcaggcaaa accagaattc agctaagaaa cagtaggcga attctaggtt tttttatttt
    23881 tcttgagagg gcgaagcaaa atgaaaattg ataaaatttt gaataacaat gtcgtcattt
    23941 cgaaaaatgg attcggggaa gaagtcgttt gtatgggaaa aggactagct tttcaaaaaa
    24001 agattgggga cgagatttcg ccagaagcgg tccaaaaaga atttgtttta agagattctt
    24061 ttgctaaaaa ccaattccag cagctgatgg ctgatattcc ggcagaggag atcgaatggg
    24121 tcaaacaagt agtcgaactg gctgaagata aactaaaagt tgaattttcg cctaatattt
    24181 atctgacatt gaccgatcat cttcattatg cgatcacacg agcaaaagaa aatatccaac
    24241 tgcctaatcc attattattt gaaacaaaaa aattttatcc gaaagaatat caagcagcga
    24301 aagaggctct tttgttcgtg aaacaaaaga cgggtgtgga actttctgag gatgaagccg
    24361 gttttattgc ctttcatttt gtgaatagcc aacaaggaaa cgaagacatg caagtgacga
    24421 tgaccgctac cacaatggta agagatatac tgagtatcat cagtaaattc ttcggcatcg
    24481 tgttcgatga agaatctttg aattatcagc gtatcattac tcatctgcag ttttttgccc
    24541 agcgttatct caaaggggaa ggatctgacg agcaagatga atttctctac gcattagttc
    24601 aaggaaagta tccaaaagcg tttcattgtg tcgaacggat caatgagtat cttcgacaaa
    24661 cgcaaaaaca ggaaatgggg atcgcagagc agatttattt aacgattcat attcaaagag
    24721 tagtaagtga gaaaaaaagc atccaatcat aaggattgtt actgtatggc aggcaaaacc
    24781 tggattgaaa aaataggttc acgaatgttt gtgactgttt ttttcaatct gggtttttat
    24841 tttttaaata aaaaagaaag aaggaaaaaa caatggataa tcaagcagtt ggtcgtcgtg
    24901 tatgggaagc ggtaggagga caaaagaatg tcaaaagttt agtacattgt gctacgcggt
    24961 tgcgattcag attaaaagat gaatctttag cagatacgca aaagttaaaa gaagacccgg
    25021 acgtgatcca agtcgtacaa agtggcggtc agtatcaagt agtgattggc agtaatgtag
    25081 cagatgttta tcaatcgatc gtggatgagg aaggtcttgc agtttcggag gatggaaaag
    25141 aagagtccaa aaatattttg aatcgtctga tcgatattat ctctagtata tttacacctt
    25201 ttctaggggc gatggcagcc gccggggtat tgaaaggatt cttatcattg gcaacggtga
    25261 tgggctggct gacagctgat agcggtactt atcaaatatt gtttgctgca gcagatggtg
    25321 tctttacctt cttacctgtc atgcttgctt ttacagcagc taaaaagttc aaagccaacc
    25381 agtttttagc agtagcaatt gctatggcac ttgtgtatcc agctatcaca gcagttgctg
    25441 gtgcgggaga agctatcagt ttctttggga tcccagtgat tctttcacca tctggttata
    25501 cttcttctgt cataccaatc attctagccg tatgggttca aagtaaatta gagccattcg
    25561 taaaaaaagt gattccgcaa tttctgcaaa tgattttagt tccgcttgtc gttttagtag
    25621 tgatggttcc attaacattt cttgctttag gaccaatcgg gacggttgcc ggtaatgctt
    25681 taggcggtct cttcaactcg atttatggat ttagtccaat cgtagcaggt ctaatcatgg
    25741 gaagtttatg gcaagtattc gtgatgttcg gcatgcattg gggattcgta ccgatcatgt
    25801 ttttgaatat cgaacaatat gggtttgatg tattgatgcc aatgcttctt ccagccatct
    25861 tggcacaagg tggggctgct ttagctgtag cattgcgaac aaaagatacg aaacttcgag
    25921 cattagggat ttcttctaca gtgacttcgt tatttggaat cactgaacca actgtgtatg
    25981 gtgtgacatt acctttgaag aaaccattca ttgccgcttg tatttctgga ggtattggtg
    26041 gagctattat cggattttcc ggagtaaaag ctttctccag cagtttggtc agcttattga
    26101 cgattccgac tttcattaat acggtggatg gtgtagagtc aaatgtgaca gtagcagtaa
    26161 tagctacagg aattgctttt gttcttgcat tcgtaggtac attgatttta ggatttgacg
    26221 aacaaacaca agacaatcaa ttagaaaaca aacatgcgaa tgcaggagaa ccgattactt
    26281 cagcacggca taccttaaaa agtcctttaa cgggtaaagt actgccattg agtgaagtac
    26341 cagaccaagt tttttcttct ggcgtgatgg gaaaaggtat cgctattgat ccagaagtag
    26401 gagaactagt tgcacctgca gacggagaga tcacgactat ttttccaact ggacatgctg
    26461 tagggatcac aacgactgac ggagcggaga ttttgattca tatcgggatg gatacagtag
    26521 aattaaatgg gaatggcttt gagatcttag tcaaacaagg ggatctcgtc aaagcaggag
    26581 atttattgat tcgttttgac atcgaagcaa ttagagcagc tggctatagc gtaatcactc
    26641 cagttgtcat tacgaatact gatgcatttg ccgatatact tgaattagac caaaaagaga
    26701 ttattgcaaa cgaagatgtt ctggcaattg taaaataagg aggaaatcag gatgcaatca
    26761 cgtttttcag aaaaattttt atggggtgga gcaactgctg ccaaatcaat tagaaggggc
    26821 ttatgatcga gacggaaaag gactatccgt tgcagatgct atgcctggag gtaagcagcg
    26881 ctttgcgatt atcggcagtg aagaatttga ctggacgatt gatcaagaaa aatatattta
    26941 cccgaatcat cgaggaatcg atcattacga tcgtttcaaa gaagatcttg ccttgtttgc
    27001 ggaaatggga ttcaaatgtt atcgtttttc gattgcctgg acacgaattt ttccaaatgg
    27061 agacgaaggt actcctaacg aagcgggatt agaattttat gatcagctga ttgatgagtg
    27121 cttaaaatat gatattgaac ctgtgatcac gatttctcat tacgaaatgc ctcttcattt
    27181 ggcaaaagaa tacggtggct ggaaaaatcg aaaactgatc gacttctatg aacgatttgc
    27241 tcagacagta ttagagagat acagcagtaa agtcaaatat tggatgactt tcaatgaaat
    27301 caattcggcc tttcactttc cagctttaag tcaaggatta gtaaaaagca atggagcagg
    27361 agagtaccaa aatattttcc aagcatggca taatcaattc gttgcaagta gcaaagccgt
    27421 aaaaatcggc cacgaattgc gttcggatat ccaaatcggc tgcatgatta tctatgcaac
    27481 aacttacagc atcgatgcca atccagtcaa tcaggcagca acaatgatcc aaaaccaaga
    27541 attcaatttt ttctgcacgg atgtccaagt acgtggagaa taccctgcat acacagcgcg
    27601 tacccataaa aaatatggag tcgatccgga aaaattagag caaacggaaa aagacttcca
    27661 gttgctaaaa gaatatccag tggactacat cggatttagt tattatatgt ctacggcaat
    27721 caatgagact gatccacaag cagcaacttc agaagggaac ttgctgggag gagtaaaaaa
    27781 tccgtttctt gaggcaagtg aatggggctg gcagatcgat ccagaagggc tgagaatcgc
    27841 attgaatgaa ttatacaatc gttaccagaa accattattc attgttgaaa acggtcttgg
    27901 agccattgat catatggaag aggatggcac gattattgat gattatcgga tcgattattt
    27961 acgtcgtcat atcgaagcga tggctaatgc agtagcagac ggtgtagatt tgatgggcta
    28021 tacgccttgg ggatgtatcg atcttgtcag tgcatcaaca ggagaaatga gtaaacgtta
    28081 cggctttatc tatgtggatc tagacgatga aggaaatggg acactgaatc gatcaaagaa
    28141 aaaatctttt gattggtata aagaagtaat tgcctcaaat ggcgtaaact tgtaattaaa
    28201 gcagctgaat gaaaaagaac acacttctca catgtgattt gtgttctttt tttatttttt
    28261 atgtgctatt cttttagtaa tagtaaagca aagggtgacg ttatgaaaag gacacgtgta
    28321 ctatacttgg aagtcgcaga taaaattaaa gaagatattt tctcaggaaa atatccagtc
    28381 ggttcgatgt tgccgacaga aagtgaatta gaagaactgt ttcaagtcag caaaatcact
    28441 gtccgaaagg caatcgaact gctggcatca gatgaatatg tcgcaaagaa aagtggtcgt
    28501 gggacgacgg ttctaagcaa tcgtccttac aatcgattat ccaaagcggc aagctttacg
    28561 caaatcttgg agaactcccg atatgaagtc cggaaagaaa cgctggattt gcaattggtc
    28621 catataaaga aggaagatcc tttgttttcc tttttcggtg agcaagctgt ttgttttaag
    28681 cgcttatatt atttgaacaa tcagccttat atttattttg ttcattatct tccacaagaa
    28741 atgatgcaat tcagcagaga agaatttggc aaagaatctt tgtatcgttt gctgacgaaa
    28801 aaaggatata ctattgactc attttctgat gatttctcgg cagtattttt aacagaagaa
    28861 gaacaaggaa ttctgaaaac agacgcacag atcggcatca aacggatacg gaaatcgtct
    28921 acgcaaattg gggaaatgat tgaatattcg gaggcggtct accatacgga gctgcatcct
    28981 taccatattg attatgaaac ataaataaaa aaagaggttg tgaaaaagct gccctgcatc
    29041 aagtaataag aacagccaaa caaaaatagc ttctcatatt tttcgtttgg ttaggcttat
    29101 taccgcagat acacggctgt ttctggatta ccatttattc tggataaggg ggtgtggcac
    29161 gattttcaga aggaatctat gaaccaatca ctgtattgat tttagcttcg atggtaaccc
    29221 cattcggctt gacgatcgct tatttcttag ggaaaatcat tcgtaaaaat atactcaatc
    29281 gtcaagaaat cgatacattg aagaccgctt ttcctatggg gatctgtcag atcacagagg
    29341 gatgtttccc tatcgtattg aacgacttgg cacgcaatgt catagcaacg ggagtcggtg
    29401 ggacagtagg tggcggatta agcatgtttt ggggcgcaga tagccgtatt ccggcatcgg
    29461 gaatgtttgc agtcgctacg atgaccagac catgggcatt cattggtgca ttgttagctg
    29521 gttcttttgt cacaggaata accattttat tattgaaaaa accagtagat ccgaatgcag
    29581 aaatcatcca agaagaaaaa gaggaagaag acatatcttg ggatgatttg acaattagtt
    29641 aggagaggca gaatggatta caagaaaaaa gtacaataga tgtaccttga gacaggaatg
    29701 gtatgaaaga atcagagtta gaaagaatcg aatatacaga ttttggttta ggagcgatcg
    29761 aaactgtcca gagcatcggc accctccttg aaatgacgat gaaggaaaaa tgagaacctt
    29821 tcgttgtcga aagggtctgg tataccttat gtcgaaggaa aggaaacaaa aaaactttcc
    29881 tgtactattc caaaaaggaa agaaagttat tatacagtaa aaaaagaaat tattcttcat
    29941 gctagagatc agtacacgtt cgagcccaat atcaaacact agttccaggc aggcgcaaca
    30001 ggtgcggtta tcactgaatt ttcttccagc agtgacgatt caagtgatat ttttacaaat
    30061 ccagctattt atcgttaaca aaaagatttt taggagaagc tgatgaacaa aaatgatcaa
    30121 attttgatcg tttagttcgt cggctttttt atttagagaa acatcattta tcagtgaaac
    30181 ataacacgag aacaaaatga ttttttcgtt aataaaagaa acaagtattt gttgtttaaa
    30241 ataatacacg ttattggcat ataggacgaa atcccagtat cttctataag taagtcattt
    30301 catatcgatg aaaacacgtt atttattttt gttttatcta taaaatggct gaaaaatgat
    30361 ttttttctaa atactatata tagtggcgtc tatcctatat accactaaaa aactacaaaa
    30421 tctagtcttt tattttaaaa aaacacgtgt agaatgaaga tagacttttg agttagggag
    30481 tgttttttta tgaaacttac tgttttttgc ggttcccgtt ttggtaataa agaaagttac
    30541 aaatttattg cgcaaacgtt aggaaaatac atggcacaag aagaaatcga actggtttac
    30601 ggtggttcat actccgggat tatgggagtg attagtaaaa cagtactgga aaataacgga
    30661 aaagtcacag gtatctatcc gaatgggctt tttgaaactg aattgcctgg aaaagatgtt
    30721 actacattta ttcctacaga aacgatcgat gaaagaaagg tattattatt tgaaaaggga
    30781 gatgctgtcc tcgttttccc tggaggtcta ggtacattgg aggaattctc acaacttctt
    30841 tcttggatag caatcggtct gacgcccgac aagccaatcg gaatattgaa tattggcggt
    30901 tattacagcg gcttgcaaca attattagaa acttttgcaa aagaggattt tatggataaa
    30961 aaatggttgg atcaagtaat cttttccaac aatccattgg aactggtcaa tgaattacga
    31021 gcagtatcta ttaaaaatca gttattattg aaggaggcaa aataatgagt caaatatatt
    31081 tagcaggacc atttttttca gaggagcaaa tcgatcgagt aagtcgtatc gaaaaagcgt
    31141 tggaggaaaa caaaacagtc acaagtttct actctccacg tcatcaccaa gaaagcaatt
    31201 atgaactatt cagcgctgga tgggcgcagg aagtatacga aaaagatatg gaagaactga
    31261 caaatgcaga atttgttgtt gctatccttg actttgagca tcaaacaatc gatccaggaa
    31321 cggcttacga attaggtgtg gcaactatgt tgaaaaaacc gatgatcatt gtacaagaag
    31381 agacggtacc tacgaacttg atgattacac aaagtctcca tacgtattta aaatcagacc
    31441 aagctgtacg tgaatatgat tttgaaacat tgcctgttga aacttacgta ggcgagtact
    31501 tatagaagag gaagggttgt atgtcagtga ttttgatttc aggaacgatt ggtgcaggta
    31561 agagcagttt gacggatatg cttgcaaaag agattgattc taaaccattt tatgaaaatg
    31621 tcgaagataa cgaagtattg ccgctctttt attcaaatcc tgagcagtac gcttttttat
    31681 tgcaaatctt ctttttgaac aagcgatttt tagcaatgaa aaatgcttta gtgaacgatg
    31741 acaatgtgct agaccgatcc atctatgaag atagcctgtt gttccatcta aacgctgatt
    31801 taggtcgggt aacggatatc gaagtacagc agtacgatag cttattagat acgatgctaa
    31861 acgagttaga tgatgttgct ccaaaaaaac ggcctgattt gatggtccat atcaaagttt
    31921 ctttggacac gatgctagaa cgtatcaaaa aacgtgggcg tgactatgaa cagctagaaa
    31981 gcgatgagac gttatatacc tattatgaaa cgttgaacac ccgctataat caatggtatg
    32041 aagattttga tgtatgtcct aaaatacaag tggatgggga taaattcgat tttgtagaaa
    32101 atccagaaga tgcaaaaaag gtgatccgtc agatccaaga aaaattagct gaattggaag
    32161 gaaagcctgc tggaaaatat tttacggata accagcatgc aaaaggtcaa gaattaggct
    32221 aaaaataaaa aaccaaacga cgggaaagaa aaattactac gtgatgtaga ataagccttt
    32281 ctatcgtttg gttattttat attcaaatat ttgaaataaa taaatcttta ctctttgatt
    32341 tgtaaactga aatgatccac tgtttgtttt actgatgaag cttgatcaaa ggaagaagct
    32401 gagtttgaag ttcataaaaa tcagccatag tgagttttgc ttggatttgt ttatcttctt
    32461 ctacttttta cataattttg aacaggataa gattcaaatg ttttaaatga atctttgact
    32521 agtttgtgac agttgtcgca tatgctcaag cttccttggt cattaaattc tgtatggata
    32581 tttttgttgt tattacatgt acaataatca ggactatcga tcatccattt cgtctccttt
    32641 acaatcatct tgcttcttta ctatagcaga aaagaagggg agaaacaaaa gaagatccaa
    32701 cagacggcac tatagactgt agatgttgcc atcctcatcc ttcaaacggt aattaccgat
    32761 ttttgtaaat atgactgttg tttctttttt attttctttt cctgaaataa tatattcgtc
    32821 atctttggta gtgatcttga tatctgtcag cactgttttt tcttcagatg attcagaaga
    32881 atcagttcct tgtgtctgat acgtgcgtgt ccattccatt tttttatctg taaatgtcaa
    32941 ctgtcccgcg gataaatcca tatctttacg tacttttagc gtatatacac tgttggtggt
    33001 atctgctttt tccgctttta agacagaaga atcggttgtt tcgtccgagg attggtcaga
    33061 gccgccaaac aaatcacagc cagtcaatgt caaaagtccc gcagacaaaa taaatagtac
    33121 gattaatttc ttcaaaatac ttcccccatt tgataggata aagaaattat accattggaa
    33181 tgtaaaactt caaacggatg gaagagaaaa acgcacaaaa cacccacaaa tagagtaaat
    33241 tttagaataa aattttaaaa aggcaaaaaa tccgccactt tcagaaagcg ttttaaaaaa
    33301 gtgttgcaat cttttaaaaa aatgctatat taaaaatgaa ccggttcaaa tacttgttta
    33361 agtataatta atagtgagag gaagttgctc tactgaagac tttagttata tggatattga
    33421 tgacaagcaa cactatgcaa agaatgcggt catcataatc gaacaacaag aaagagaaat
    33481 caaatgtaac tggcaggagt gagaatgtat ggcatcgatc cgtgatgtgg ctaagatggc
    33541 tggtgtttca gtgggaacag tttcccgtta cttgaatggt cagcaactga aggaaaagaa
    33601 catgaaaaaa atagcagaag cgatttcagc gttggactat aaagaaaaca tcattgctaa
    33661 agggttgaag aataatcgaa gcttctctat tggacttttg ataaacggga tttccagccg
    33721 ttttgggtcg gaagttgtct cagggatcga aagagtagct gaagaaaatg ggtatagctt
    33781 attattaagc gggtttgccg atcagccaga aaaaattgaa caaaaaattg aatacctgat
    33841 gaagcatgtg atcgatgggc tgattgtctt cttatccgaa gaagagtggt ctggatttga
    33901 aatgctcaca aaaatgagta ttccagttat cgcattgaat tgtccagaag gaccagcagg
    33961 tattgataca atattagtga atgatcgaga aagtgtagca aaagtgatcg ctcatcatgg
    34021 tgaacaagga cataaaaaaa tcggatttat tgctgccact caaacagact acgtagcaag
    34081 agaacgttta gcaggagcca aagaagcggt ccaaaatgat ccatcgatcc aattagaggt
    34141 ctttacagga gactattcta gaaaaagcgg ctattacggg gcaaaagcat tattagaaaa
    34201 aggggcaact gctatttttg tcagcaatta caatatgtcg attggtgcca tcgaatgttt
    34261 caatcaagaa ggtgtcagaa tcgggaagga tattgcgttc agtcactatg attatttaga
    34321 taaaaacgat ctgagtatcc ttccgaaaat cacgatcacg ccaccaacag ataaaatcgg
    34381 ggaacgagca gcagaacagc tgttaaaacg aatcaaaaaa gaagaggaag aaaatgaaaa
    34441 agtgattgtg atggaaaata tcatacatgg aatcaactaa aacgaactaa aaaagaaaaa
    34501 gaggtgcttt gatgaaaaaa tatcaatttc cagcaaattt ttggtggggt tctgcagcaa
    34561 gtggcccaca aacagaagga aggatttcag gcgatggaaa aggagagaac atttttgatt
    34621 attggtacaa aaaagaaccg caaaagttct ttgatcaagt aggacctgaa aaaacctcgc
    34681 aagtttatac gaagtatcaa gaggatgtac agttgatgaa acagactggg cataattcat
    34741 ttcgaacatc tatccaatgg agccgtttga tacctgatga atctggaaca atcaacgaag
    34801 aagccgttcg tttctatcat cgttattttg atgagttgat tgaaaacggt atcgaaccct
    34861 ttgtgaatct gtatcatttc gatatgccga tgtatctgca agaaaaaggc ggatggttga
    34921 accgagaaac ggttgaagcc tatgaaaaat atgccaaaag ttgttttgaa ttgtttggtt
    34981 cgaaagtaaa aaaatggttt actcataatg aaccaatcgt tccagtcgaa gcaggttatt
    35041 tatatcgatg gcattatcca gaagagtcag atatgaaaaa agcgatccaa gttggctatc
    35101 atgaagcgtt ggcttcagcg ttagccatca aatcttacca tgaaatggag caaggtggtg
    35161 ttattggtat catcttgaat ctgacaccaa gctatccaag agacgaatca aaccctgaag
    35221 atgttaaagc agcgcaaata gcagatgcgt tcttcaatcg ttcattcctt gatccagctg
    35281 taaaaggcga gtttccaagt gaattgattg agatagtaaa agaacttgat ctcatgcctg
    35341 tgatccatgc agaggatttg acggttatca aagaaaatac agtagatctt ttaggtatca
    35401 attattacca gccacgccgg gtaaaagcaa aagaaacacc aatcgatact aaaaacggtc
    35461 caatgcctga ggattatttc gacaattatg aaatgcctgg tcgtaaaatg aatccttatc
    35521 gcggatggga gatctatgaa caaggaatct atgatatttt gatcaatgta cgagatcatt
    35581 atggaaatat cgactgcttt atttctgaaa acggtatggg tgtagaaaac gaagcacgat
    35641 ttatcaaaga agatggcatg atccacgatg attatcggat cgaatttgtc caatctcatc
    35701 tgaaatatgt ccatcaagcc attcaagaag gtgccaattg tttaggctat catatgtgga
    35761 catgtatgga taactggtcg tggaccaatg catataagaa tcgatatggc tttatttcag
    35821 tagacttagc acaagatgga aaaagaacaa tcaaaaaatc gggtcattgg ttcaaagacg
    35881 tatcagataa aaacggattt gaggcttaag aaagaatagt tgagagcaaa tgcgtgaaat
    35941 tctccattct taccattttg tgaaaaagaa gttcaattat ttacgatctt tttcaacttt
    36001 tttgtctttc tatttcattt tttgtatata atagaatagt caaaattaaa taggagtgga
    36061 ggaaagcatg aggaaaaaaa gaccgtttct tctcttttgg ttattcggtt ttttcttact
    36121 gcttatttta tccgcagcat tttcaggaaa aatgacagca gatcttgctc agaaagaaac
    36181 agaaaaaatc gtagcaagca aagaatcagt agataatttc aaaaaagaat tctcaactta
    36241 ttggtgggcg gagtataaaa taactgccaa ttacgaaaaa ggcgaattgg acatttattt
    36301 gccaaaagga aaagaagaaa atcaatttat taatatcgat gacgtactta gtgcgatttc
    36361 tctcagacaa tatcaattta aagacaagca tttgaccgtt ttgcttttga ctcatgatgg
    36421 cgacaaagta gcccgtttga aaaatggaaa agtgatcacc gcttattctg aaaaagtaga
    36481 caaaaaagtg gtcgccgatc gtttagcgga atggaaaaag acaaatgaac aagtcaagaa
    36541 aacaactgtc tcttctcaaa aaaacgagtc gacaggaaca agtccttcag attctggcag
    36601 tggacaaacg ataacgggaa gtgaagaaaa tcaagcctca attattacga atgagtcatc
    36661 acaaggacaa tatcaatcct atggacaatg aaaaa
//
LOCUS       DACEXQ010000006        31793 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.006, whole genome shotgun sequence.
ACCESSION   DACEXQ010000006 DACEXQ010000000
VERSION     DACEXQ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.006"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(108..638)
                     /locus_tag="IXH04_000216"
     CDS             complement(108..638)
                     /locus_tag="IXH04_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAQ1850853.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            743..1294
                     /locus_tag="IXH04_000217"
     CDS             743..1294
                     /locus_tag="IXH04_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1850854.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(1447..1920)
                     /locus_tag="IXH04_000218"
     CDS             complement(1447..1920)
                     /locus_tag="IXH04_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1850855.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            2058..3530
                     /locus_tag="IXH04_000219"
                     /pseudo
     CDS             2058..3530
                     /locus_tag="IXH04_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
     gene            3527..4237
                     /locus_tag="IXH04_000220"
     CDS             3527..4237
                     /locus_tag="IXH04_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAQ1850856.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            4589..4873
                     /locus_tag="IXH04_000221"
     CDS             4589..4873
                     /locus_tag="IXH04_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAQ1850857.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(5154..5573)
                     /locus_tag="IXH04_000222"
     CDS             complement(5154..5573)
                     /locus_tag="IXH04_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAQ1850858.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(5575..6159)
                     /locus_tag="IXH04_000223"
     CDS             complement(5575..6159)
                     /locus_tag="IXH04_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAQ1850859.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(6206..7681)
                     /locus_tag="IXH04_000224"
     CDS             complement(6206..7681)
                     /locus_tag="IXH04_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAQ1850860.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            7862..8173
                     /locus_tag="IXH04_000225"
     CDS             7862..8173
                     /locus_tag="IXH04_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1850861.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(8287..10623)
                     /locus_tag="IXH04_000226"
     CDS             complement(8287..10623)
                     /locus_tag="IXH04_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1850862.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            10765..11433
                     /locus_tag="IXH04_000227"
     CDS             10765..11433
                     /locus_tag="IXH04_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1850863.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            11436..12479
                     /locus_tag="IXH04_000228"
     CDS             11436..12479
                     /locus_tag="IXH04_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1850864.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(12716..13597)
                     /locus_tag="IXH04_000229"
     CDS             complement(12716..13597)
                     /locus_tag="IXH04_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1850865.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            13756..14483
                     /locus_tag="IXH04_000230"
                     /pseudo
     CDS             13756..14483
                     /locus_tag="IXH04_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
     gene            14639..14956
                     /locus_tag="IXH04_000231"
     CDS             14639..14956
                     /locus_tag="IXH04_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1850866.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            14949..15557
                     /locus_tag="IXH04_000232"
     CDS             14949..15557
                     /locus_tag="IXH04_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAQ1850867.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            15573..16403
                     /locus_tag="IXH04_000233"
     CDS             15573..16403
                     /locus_tag="IXH04_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAQ1850868.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            16541..17029
                     /locus_tag="IXH04_000234"
     CDS             16541..17029
                     /locus_tag="IXH04_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAQ1850869.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            17080..17952
                     /locus_tag="IXH04_000235"
     CDS             17080..17952
                     /locus_tag="IXH04_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAQ1850870.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            18128..18490
                     /locus_tag="IXH04_000236"
     CDS             18128..18490
                     /locus_tag="IXH04_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ1850871.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            18499..19251
                     /locus_tag="IXH04_000237"
     CDS             18499..19251
                     /locus_tag="IXH04_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1850872.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDVGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            19265..21067
                     /locus_tag="IXH04_000238"
     CDS             19265..21067
                     /locus_tag="IXH04_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1850873.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            21344..22384
                     /locus_tag="IXH04_000239"
     CDS             21344..22384
                     /locus_tag="IXH04_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ1850874.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(22436..24580)
                     /locus_tag="IXH04_000240"
     CDS             complement(22436..24580)
                     /locus_tag="IXH04_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1850875.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(24937..26310)
                     /locus_tag="IXH04_000241"
     CDS             complement(24937..26310)
                     /locus_tag="IXH04_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1850876.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFDGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(26310..26705)
                     /gene="rbsD"
                     /locus_tag="IXH04_000242"
     CDS             complement(26310..26705)
                     /gene="rbsD"
                     /locus_tag="IXH04_000242"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAQ1850877.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(26733..27650)
                     /gene="rbsK"
                     /locus_tag="IXH04_000243"
     CDS             complement(26733..27650)
                     /gene="rbsK"
                     /locus_tag="IXH04_000243"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAQ1850878.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(27758..28771)
                     /locus_tag="IXH04_000244"
     CDS             complement(27758..28771)
                     /locus_tag="IXH04_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1850879.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(28852..29451)
                     /locus_tag="IXH04_000245"
     CDS             complement(28852..29451)
                     /locus_tag="IXH04_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1850880.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            29637..30338
                     /locus_tag="IXH04_000246"
     CDS             29637..30338
                     /locus_tag="IXH04_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1850881.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            30348..>31793
                     /locus_tag="IXH04_000247"
     CDS             30348..>31793
                     /locus_tag="IXH04_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ1850882.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEGL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAF"
BASE COUNT         9803 a         5729 c         6452 g         9809 t
ORIGIN      
        1 ccctcggtat tagctcaaaa aacggcaaaa catgaggaac tatttttccg tttctttctc
       61 taatactcag gtcagaacgc tgatttcaca acctctcttt cacaacctta ttttactcgt
      121 ttataatttt catcgaagta ttttccgcta cggatatcat ctggcattcc atcgtatggt
      181 tcttcgctaa taacatcttc attgtctata cctgcatcac ccatataagt gatcgtagca
      241 aattcaggaa caagtaattt tggataagtt tcatattttt cacgagctgc ttccatttct
      301 gcgatatgtt cgttttggaa agtaacagtg atttctggat gttcttcaac ccatttaggg
      361 aaaaagatca ttccgtgaag tttcttttca tttggcataa agtccaatgc cacgtctgtc
      421 cctgttggct ctgtccaagt gattttgaat acgccgtccg tcaatttaac gatatcagct
      481 tcttggtctt ttacccaacg gcctttgacc attccgccat ggatacggta atcaacagta
      541 tggtcatttt tggcatacca ttcatattcc caaccattgt catacgtata gataaagtga
      601 gttccaataa aatcttccaa ttctttaaat tctttcatgt ttaagcatct ccttataaat
      661 taaaacatgt aataaactac atgttttcga ttactcattt atgatagaat ggatttaaag
      721 atttgtcaag gagggaaaac tgatggcaag aaaaaataca cttcgatata ttttgttagg
      781 gttattaagt aagaagaaga tgacaggata tgaattgaat caatcgttca aaaatgaaat
      841 aggagaattt tggcaagcaa aacatagtca gatttatcct gaacttgcaa aaatggaaga
      901 gcaaggaatc attcagcatc aagtagagat cacaggtgaa aaattggaaa agaaagttta
      961 tgaattaaca gaagaaggga aggaacaatt ggcagagtgg attcatacac ccaccaatga
     1021 actaccggtt aatcgagacg aatttgttct gaaattgtat ttcgtcaaag acgtgaatga
     1081 tccggcatta gtagaaatta ttcagcagca gcgtgacctt catgaagaaa aacgacttca
     1141 tctattagag cgaaaaaaaa cgatctttcc tactgaaaaa gaaaaacatg aaaattacgg
     1201 acactacctg attttagcgc atgcgatcaa tcgggaaaca gaatatgcaa attggctaac
     1261 agatgtatta gaagaaagta accgaaaaaa ataaaaaaat atccgaacag tgatggagaa
     1321 ttcctttcta tatgaaagaa acgcagatca ccgttcggat attcttatgt tcaaacaggc
     1381 cttttttaaa gtatttatgt agcatctact tattaaaaag cttggtactt aggagattag
     1441 tggcgtttag agttctttta acaactgttc gtagtcagct ttttcatcga caggaaggag
     1501 cttgtaagtt aattcgtctg tctcgttgtc atgaatgatg tacatcttag cattctcttc
     1561 tttgaagtta ccgatttgta aaatcgtata gcgttcgtcg atcaattcga tatctttgat
     1621 tgcttttgaa aaaactttac ggacatggtg cattttcttt tggcggattt ctgccttttc
     1681 tgcagctttt gacaaagtaa atccgtcatc taaatatttt ttgatcagtt ctacttttat
     1741 gactgtaggt aaacgatatt ttctaggaca attcgcatca gcttcgatcg aacgaatgaa
     1801 tcccttttgt tcccaatacc ttaattgacg tggagacaca cctacgatat cacttaattc
     1861 actgatgcca acaaccaatt gatcattttc caacaaacgt ttaagggaag ccccaaccat
     1921 ttttgtcact ccttttgtcc tcttgcttcc cttaattata tccgtattaa cttatgtgtc
     1981 aagtaggttg acaaatagcg aatttaggac taaagtattc tagtagtttc agaaaaaatt
     2041 cagaaagaag gagaaatatg agtaacaacc aacccgtaga tatttacgga aaaccatata
     2101 atcggacact tttagttgtt gtcttattaa ttggtacatt ttgtaccgtt ttgaatcaga
     2161 ccttattgac gacagctttt cctacattga tgaaagcttt cgatatttca gcgtctgatg
     2221 tgcaatggtt aacgactggt ttcttgctag tcaacgggat catgatccca ataacagcat
     2281 ggctgatcaa taaattcagt tcaagaaatt tatatctttc tgccatgacg atctttttga
     2341 tcggaacgat tacttgcttt acagcaccta attttagtac attactaatc ggtcgtttga
     2401 tccaagcttg cggtgtggga gtatcgatgc cattattgca aaatatcatg ctatctattt
     2461 tcccgccaga aaaaagagga tcagcaatgg ggatgtcagg gatcgttatt ggacttgctc
     2521 ctgctttagg ccctacatta tctggttata tcattgataa ctaccattgg cgtgacttgt
     2581 ttggtatggt actgccaatc gttgttcttg tcttggtttt agcattcttc ctaatgaaaa
     2641 gtgttattcc attgtcaaat cctagcattg atattttatc cgctatttta tcaacgattg
     2701 gattcggtag tttactttac ggattttcta gtgtaggaga tgacggctgg ggaagcttga
     2761 aagtaattgg tttcttagtt gttggtgtga ttttcatcgc cttgttctca tggcgtcaat
     2821 tacatttgga acatccattc ttggaacttc gtgtattcaa atcatctaca ttcacgattg
     2881 cagctgtttt agctggtgta acaaatatgg cgatggtagg ggcagaaatg gtcgtaccat
     2941 tgtatattca aaatattcgt ggagaatcag cattccattc tggcttgatg ttgttgccgg
     3001 gtgcattgat catgggggct atgatgcctg tgacgggtgc gattttcgat aaatacggcg
     3061 cgaaacgttt agccatcaca gggatgttca ttttgacagc ggcaactgca ccatttgctt
     3121 tcttgacaaa aacgactcca gttttatata tcgtgatcct ttatgcgatc cgtatgtttg
     3181 ggatctccat ggtcatgatg ccggtaacga cttctggtat gaatgcattg ccaaataact
     3241 tgatcagtca tgggacagcg gtaaataata cattccgtca aatcgcaagc tcgattggta
     3301 cagctatttt gatcagtgtg ttgacgaatg tgacaaaagc aaatatccca agcaaatctg
     3361 tattggataa tacgccatta gcttataaag atcaggcaat caatgccaca ctgtaaggtt
     3421 accatgccgc gtttatcgta gcgatcatct ttgggattct tggctttatt attgcctttt
     3481 tattgaagaa aaaagaacct gttctaatga aagcggggga taatgcatga ttattggagt
     3541 tttgattgtc agcttgcttg cttttgcttt aacgaacgtg ttcgctaaaa aaacatggca
     3601 aaccttttta tctttgattt tcggactaat cttcgtcgct tcactaagtt tgatcgtcgc
     3661 taatttgtcc aatcactttg gaatggaaaa agtaacggaa acaaaaacag aaaaaattgt
     3721 ttccagtgct gattcccaag gagcagatat gcttttgtat aaagctttag ggaatggaaa
     3781 agaaaaagtg tatctttacc gaacaaatga aaaacaagaa aaaccaaaag caactggtac
     3841 ggataacgaa acaaataaag tagaaaagac agacggcgat gctgaaaaag tgacgaaaac
     3901 tacttattgg gaatacaaaa acgacatgta taaattctgg ttcaatattg ctgataacaa
     3961 tcacgaatat gacagccgag tgaatacatt caaaatccca gagacatggg tagaattaag
     4021 tacggaccaa gcagccaagt tagctgaatt agtgaagaaa cagcaaagta cgatggaaag
     4081 tgaagcaaaa gcttatgttc aagacggtat ggtaaaagca atgactgaaa atccaaaaat
     4141 gagcaaagca gagcaagaac aacgtacgaa agccttagct gctgaattcc aacaacaagc
     4201 attcgcaaaa ttagtgaaag aagcaaaagg cgaataatat tttgaacacc atgtaggagg
     4261 ttgtgaaaaa gctgctctgc atcaagcagt aagaacaatc agataaaaat agcactccat
     4321 atttttcatc tgattgggct tagtgccgta gatgcaagct tttgaacacc gtttaaggaa
     4381 gaggttgtga gcctgccgtt taactctgag tcacaaggag caatttcaaa aaacagcttc
     4441 tcatgtttgt tgactattgt gacttgtgac cgaggagttg gcaggagaac accgtttatt
     4501 cgaaaaaagg aactgcgaca agacttttgt cacagttcct tctttttttt gctatgctat
     4561 actagatgta aaaaatagga gaaataaaat gagggaaatt tattcttttg aatcaaaaat
     4621 ctatgcttcc gaagtcggca agggcggggc atatattatt tttccgtatg atataagaaa
     4681 agaattcggt gctggaagag ttaaagtcca agcaactttt gacaatgtgc cgtatgaagg
     4741 cagtatcgtg aatatgggcg tgaaaaatcc agatggttca gtctgctaca tccttggact
     4801 acgaaaagat attcgtaaaa acatcggaaa agaaatcggt gatattgtag cagttacagt
     4861 gaagcaaaaa taaaagaggt tgtgacaaaa gacgtgtcac aacctcttga taaacggtgt
     4921 tcaaaagcca gtttctcggt agtaagtcaa atttatgcaa aaatgtggaa acccatgttt
     4981 acaaaatttt tcttactact cgaaacgaag cggctttatc acaacctctt gataaacggc
     5041 gttcaatcag ctgtccctcg gtattagctc aaaaacggca aaatatgaga agctattttt
     5101 ccattttttt ctctcatact caggtcataa cgctgatttc acaacctctt ctttcataat
     5161 ggtttttcac catctttata ataaactggt tctttgatat ggaaatctat tttttccaac
     5221 caatctaaaa tcttgtaacc taactcttct gacatcggaa aagaggcgtt gatttgtgca
     5281 ttggagatat ggtcaacatg gataccggaa gtgatcgtac aacttcccgg aacggcagtt
     5341 ttgatgatca cagctatttt ttcggctaag acatcgtctt tatggaaccg accatcgtgg
     5401 ctagggaaac gtacagtttg catctctgta tcacgagaaa atgtagtgac tgttccgata
     5461 tgtggtgtat ctccgcctgt taaagtgatc aataagtctt taccgattac tttaacagca
     5521 gcttggatcg tgaaacctag tttggttact tcaactatcg tttctttttc catattaatc
     5581 accctgccaa cgttttgaat gctcatttac aatttcaaat tgatccaaaa gtttcattgt
     5641 atggtgatta acaagatctt ggatcgtttg aggctgatta taaaaagcag gaattggtgg
     5701 tatgatttgc acacctagtc gtgctaattt agtcaagttt tccaagtgaa tggcatttaa
     5761 tggcgtttca cgaggaacaa tgactaactt tcgttgctcc ttcattgtta catcagcagc
     5821 acgtgcaatc aggttgtcgc ctaaaccaac agagatacct gcgactgttt tcatacttgc
     5881 cggtacgatg atcattccat cggtcaaaaa tgatccgctg gcgacagcag cacctaaatc
     5941 tttgtttgaa taatggtaat caaacaggct ttcgaattca gttagtgata agtcggattc
     6001 cagctttaag ttctgttttg cccatggact gatgattccg tgtgtttcta tcatcggata
     6061 ttcttttagt ttcttcacta ggttgatggc ataaatggtg cctgaagcgc cacttacacc
     6121 aatcacgatt tttttcttcc gttgtgccaa ttgttccatt ttccgcactc ctttttatga
     6181 tgtcaaaaca gttgcagatc attcattatt tcaaatattt ttcccaatct tctacttctt
     6241 ggaattttgc acgtacaaaa tgttctttca tatcgaaagg aactgtaccg tcaaagatag
     6301 tttttgcact tatcccacgg aaacgaatag agccaggatt atattctggt aattctgatg
     6361 ggtccaatgg atggttcctc attcctggga tcacgatgat atctttatct ccttggaaac
     6421 gagtgttgaa agtccaaact acatcgttca tgtcgaaaat atcaacatct tcatcgacta
     6481 aaatgactgt cttcaattct ttgaaggctg ataaagcaag aatagctgct tgacgctgaa
     6541 taccttcatc cgcttcgttt tctttacgga tttgcatgat ggtcatcaat ttaccaccac
     6601 ctgcaggagg attgtagaca tttactactt ttcctggaat agctttatcg accaaggcaa
     6661 gaatacttgc ttctgtagga ataccggcca tagatacgtg ttcttcactg ggaccaatcg
     6721 ttgtttgcat gattggattg tcttttcggt gagtaactgc tttgactttc actacttgta
     6781 cagctggatt agcatcccca gtatagccag gaaattctgg cattgctttt cctgagttcg
     6841 tgttgatatc ttcttgcatt gtttcatttg gcatgatttc tgcttcaatg atgaattcag
     6901 cacgagcaat acccacttca tcgactgcta taccatcaac gatttgaata ggttcgttac
     6961 gcaaagctcc tgcacaccat agttcattgt agccaagtgg agtggtaggt ggttcgaaag
     7021 tcgtgccaat ggcaattgct ggatctaaac caatattgat agtgataggc attggtttat
     7081 tcaaggcttc gaattgtttt tggaagtgac caatgtggcg tccgccaggc atgatgtaca
     7141 taccgatcgt atctttatct tctagaacca tacgatggat cgttacatcg ctcattgttt
     7201 tttctggatc tgatcctaag actacaccca ttgtgatgta tggaccagca tcttcaggag
     7261 tatttgtagg tgcaggcaaa atgttgcgaa tatcgaagtc tgcatcggta gctagatgaa
     7321 cgacttcttg tgctggagcg tctgctttgt ctactttcac aggtttgatt ggattttcta
     7381 cagcgtcttt caagaaacga ccaagtgttc gataatcatg gtgcaaaatt tcacctacac
     7441 gtttacgact agccattgtt ccgatgttca cacgtgtgcc ggggaaacct ttgatattgt
     7501 tgaaagtcat agctggtcct tcttgagtcg gacgttttac tgtaccgcca gcaccgatat
     7561 aacggtaaac accagataat tctgcttcag gatcgatttc aatatctgtt tcgtgatatt
     7621 gacctggaag tgtttttagt tcttcgatga cttttcttaa atcatacggt tgatctgtca
     7681 tgagagattc ctccttagtt gtctttacct atctatagta aaaggtgaaa aagaggaaaa
     7741 caattcaact tgttcggtaa agtattctac aatggaatgg atgcgttttt ttgataatag
     7801 aataaactat gatatactta tcactaaaag aaagcgcata cttagtgttg gaggaaggaa
     7861 agtgaaaaaa atggaggagt catttcctaa agcagtaaaa gtggaaaata tagcgaatat
     7921 tttgaaagtt acatttgaaa acggagaagt aaaatacgtg aaatctcatt ggacagaaga
     7981 gataaccgat gccttgcaat ttggaaaaaa aggaagagga aaaagaaaaa atcttctcgc
     8041 actttctcgt aatatgtgga ttgggactga agtaacgatt gaagcagatg gaacagtatt
     8101 cataaatgga aaagacaggt atacgccaga agagttgtgg tacaaaggaa agaaaagtat
     8161 accagaatta taaaaaataa ttaaaaaaac agcgcgaaac aaggaacata ttacttcctc
     8221 gtttcttgct gttttctact atttcagcgc tcttatgact caaatctaaa tgtggttgtc
     8281 acaaccttat tctgcacgca aagcgatagc ggcgtctttt ttcgctgcca ttcgtgcagg
     8341 aagatgaccg ccaagcatcg tcaatatagt agagacgatc actagaataa tcgcatggac
     8401 aggatttagt tgagcaacat tttttagatc cgtcatgttg tataagatgc tattgatagg
     8461 gaacgtagct agccatgcaa taaacacacc taatatccca gaagaaatac ctaaaataca
     8521 cgtttctgcg tcaaatacgc gagtgatgtc ttttttacgt gcacctaaag cttttagtac
     8581 accgatttct tttgtccgtt caatcacaga tgtataagta atgatactga tcatgatcat
     8641 acttgttact aaagagatcc ctgcaaaagc aataaggaca taagtgatag catccatcaa
     8701 tccgccagtc agttcagtca tcgttccagc taaatcagta taaatgattt tatcttcatc
     8761 tgatttgcct ttattgtaat catctagata atccaagatc ttttctttgt cttcaaaatt
     8821 gttcggataa atcatgatac ttgaaggcaa gctgtctcca cctaaataac taagcaatgt
     8881 ttgtttggca ttttcgtcaa ctttttcagt agttaagaca tttacaccac tgtctctttg
     8941 agctttgacg atttctgatt ctttgttttc gttgacgatt tgagtcgtta attgatcgct
     9001 ataagcgatt cctggagata agagattcat tgtagaagaa gatttcacgc gtaggatccc
     9061 cgagatcttc aattcatcag aagcattctg atacatcgca tcataatctg tattagggat
     9121 aaaattccca gttggaagtt ttgtatagta agtattgttg tttactaatt tgaatgttgt
     9181 accaacgata tcatcaaaat caagtttttc gtcttctttc acatcaaatc caagattctt
     9241 caatgcgttg atattagtat tattattgcc gtctacgatc agcacgacat ctgttgctga
     9301 ggctggataa cttccggcaa gtaaagaata attgtctttt aaaaagtttt ctttgctagt
     9361 gtcaagctgt gtagggaaac ttgacactcc cacacctgtc attgctgaca tcgtactaga
     9421 taaagaaaga gattccgtat caggattttg attcgagaag ctgacaggct gtactttgcc
     9481 atttacatca cgtaaaagat tgattcctgt agtacgggta aatccgatat tattacttaa
     9541 gttaggatcg atgtctgtta cgtaatcgac gtatgtttga tcaatcttat tcgtgtgctg
     9601 tgctttatct tcttcgctga cttttgcagt aatcgttttg ctgtctggat aatccgcttt
     9661 gcttgcaccc aatccggcat cgtcacgagt ctggttagtc gttaccttag aaatagtaat
     9721 cggaaatttc gccatcgttt cagcttgcgt gttatcgatt tgtttttgga atccagaaga
     9781 aagggacaga acgatcgcga ttccgatgat cccaatactg gaagcaaagg aggtcaagaa
     9841 agtacgtcct ttttttgttc ggatattgtt gaaggaaagc tttaacgctg tccaaaaact
     9901 catctttgtt ctgcgtaagt taaattgatc atcttttggt cgttcgatgt gaggatggga
     9961 atctgttaag atcttaccat cagaaaattc aatgattcga tctgcgtatt gatgggccag
    10021 ttcaggatta tgtgtgacca tgatcactaa tttttctttc gataactctt ggatcaattc
    10081 catgatctgg atacttgttt ctgtatctaa cgctccggtt ggttcatcgc acaaaaggat
    10141 atctgggtca ttggctagtg ctcgagcgat ggccactcgc tgcatttgtc caccagataa
    10201 ctgatttggc tttttatgca tatgatttgt caagccgact cggtgcaatg catcttctgc
    10261 ccgttttcgt ttttcgtctt tagaaacacc acttaatgtc atcccaagtt caacgttttc
    10321 aataatgcct agatgaccaa tcaagttgta actttgaaaa atgaaaccga tagagttgtt
    10381 tcggtaggca tcccagtcac tgtccttaaa gtctttagta gactttccgt tgattaccat
    10441 atcaccggaa tcataattat ctagtccacc aatcacattc agcatagtcg ttttaccaga
    10501 accactagga cctaaaatcg ctacgaattc tttttgtctg aaagcgacag acacaccatc
    10561 caatgctttg gtggtggttt cgccgacttt ataatatttt tttatatctt ttaattgaag
    10621 cataatcacc cttctcccgt attgaaatat tcggtatact aataagtata tcagaacaat
    10681 gatcgtagta tacgaaataa atgttaactg aagatgaact acaagaaaaa atctagaaat
    10741 ttttcaaaaa acagagggag atcaatgaat cgtattttag tagtagaaga tgatccgcaa
    10801 ttacagttgc tttaccgttc tgtgttggaa cgagcgggct tttcagtcat caccgcattg
    10861 aatggagagg atgcgctgaa aaaactagaa gcgacacgta tagaattaat cattacagat
    10921 atcatgatgc cgaaaatgga tggctatgac ttgctagact cattacgtag caccagattt
    10981 gacacacctg ttctaatgat tacggcaaag gccgattttg aggataagaa acgcgggttc
    11041 aaattaggag cagatgatta tatgacaaaa cccatcgatg tcaatgagat gatcctacgt
    11101 gtggaagcat tgcttcgacg ttcaaagatc aattacagca gtagcttgat aatcggacaa
    11161 acaaaattag agctagatgc ttatacagtg ttacaagatc agcattcgtt gattctgcct
    11221 caaaaagaat ttctgcttct gtataaatta ctgtcttatc ccaacaagat cttcactcgc
    11281 caacagttga tggatgatat ttggggatta gatacgaata cagaagaacg aacgatcgat
    11341 gtacatatca aacgtttaag gacccgtttt gaaaagacgg aagattttca aatcatcaca
    11401 gtacggggac taggctataa ggcggttatt taaagatgaa aaaaaagcta ttttcacaat
    11461 tatggatcta ttttgctagt attgtcttca tttcgatctt agtgacactg ctgtgttttc
    11521 tcggttttat tttcttattg tctcgacatt ctatctcgcc tgctgaatcg gctggaaaac
    11581 caacgttata cccgttattc gtctttgcgg ggttcagtat ggtcgtgggt acaggaatat
    11641 cgatttttgt tgggcggcgt atcctgcatc caatcagtgc gctagggaca aatatgagtc
    11701 tggtagcaac gggagatttt tcgattcgaa tggatgaaca gcagaaagta gcagaagtac
    11761 agcaactgta taaagatttc aacgttatgg tccaagaact caatagtatc gagacactga
    11821 ggaatgactt tgtgtccagt gtatcccatg aattcaaaac accactagca acaatccaag
    11881 gatatgttca attgttacaa gccccaaatc tctctgacga agaacgccaa atatttttat
    11941 atcggattat cgaaagtatc acgcagcttt cacaattaac agagaacatc ttaaaattga
    12001 acaaactgga aaaccagcgt atccaactgg aaaagaaaga gtatcgatta gatgaacaaa
    12061 tcagagaagt aatcgttttt tttcagccta aatgggagaa agaacagcta gaattggata
    12121 tcgaacttgc tgcagtaaat tacacaggca atgaagagtt tttgtaccaa gtgtggctga
    12181 atatcatgga taacgccata aagtacaatc aaatcaatgg acagattcat atcaaactat
    12241 ttgagacagc cacagagatt gtactggaag tgaccgattc aggtgtagga atgaatgaag
    12301 agaccagaga tcgaatgttt gaaaaatttt atcaaggaga tacaagtcgt caaatttctg
    12361 ggaatgggct aggactttca ctggttaaaa aaattctaga actgcatgat ggacgtattg
    12421 attatagcag tatcgaagga gtaggaacga cagcaatgat acggttaagt aaacaatagg
    12481 aaaaagaata aaacccctaa aaattctatt gaaggttttt aggggttttt agtcgttttt
    12541 ttgtgattag ggaaatgttc ttacggttct tttggcataa aaatcgctgc tttcttattg
    12601 aactacgaga aatgttagag gtgtcattga aacaaggaca tgatgcaaga aagagagcga
    12661 aagtcatgcg tcgctctctt tttttgggtg gatagatgga cagaaaatgt agggcttagt
    12721 tttcttcttg atttaaatac tcccaaaatg tatctgaagc caaagaatgc tcaccgactt
    12781 tacgagtaaa tgcaagataa ctctctgttg ttggagaaat agggagaatc acagtatttt
    12841 cttttaatag attcttgact gttttttcca taacaatagc aatccccatg tcatttgcaa
    12901 tcatattgat gatcaagtcg attcttgctc ctttataaga gatttttggt tcaaatccag
    12961 cttcgcgaca cagatctagt acaggttgat acaagttcgt agtggggcca aggagcaaaa
    13021 agttctcctt tttcagttcg cttagatcca atttcttttt actagctaaa ggatgatttt
    13081 taggtagaac tgctacaaaa tcgtctgttt cagtaggcaa ccactcaatc gaacttggtg
    13141 gggcctggaa acttctagca aagtggatcg tgtcactatg ttcattggcg tcattgtaag
    13201 ttaattcgac actttccact tctttcaact ggactgtaaa ttcaggatgg cgtttcaaaa
    13261 actcggtgat tttggaaaaa cctttataat tgatcattgt cggtatcgtt aagatattta
    13321 atgaaagatt gttgtctttc gtataggttt caagcacttc ttgcatagca tggtattgtt
    13381 ctacgacatt ttttacatat gggagcaata agcttccagc ttcggtcaat tcgatttttc
    13441 tatgggcgcg ttcgaataat ttgacgtcta attctgtttc taacgaaaga atctgtttag
    13501 aaatatttcc ttgggtggtg aattgtcgat cagcagtttt ggtgaaattc attgtttcgg
    13561 ctaaatcaag aaaaacttcg aatttatgga tatccatcat attcctccta cttattccaa
    13621 taaagaatac tttaacacaa aaaatgaatt ggtgtaatct tctctgtctg ttacaatctt
    13681 tcttgcagcg aaagcaataa ttacagaaat ttttctgatt tttttcgctt cgtagaaaaa
    13741 gagaggagtt ttttgatgcc aactggagtt ttgatcaatg taggttccgt tttgtttggc
    13801 ggactaattg gcggattagt aggaaataaa ttaagtgaac attttaaagc acagctgacg
    13861 atggtttttg gtgtgtgttc gatggggatg ggaatctatt ccattgcgcc gatgaaaaat
    13921 atgccggctg ttatttttgc tttagtgata ggaacagcga ttggtttgat tgttcattta
    13981 ggtaatggga taaataaggg ggctgcgctg atgcaagtac ctatttctaa aatcttccct
    14041 agtgaaaagc ttggtatgac ccacgatgaa tttatcagca cattagtgac ggtcattgtt
    14101 ctattttgtg caagtggtac ggggatctat ggaagtttag attctgggat gacaggagac
    14161 agtacgattt tgatttctaa atctgtcctt gattttttca cagcagctat ttttgcgtgt
    14221 aatttagggt atgtcgtttc tgtgatagca attccccaat tcattatttt ttatattttg
    14281 ttcttattgg caaaatttat ttatccattg actacaccag acatgatctt agattttaaa
    14341 gcttgcggcg gattcttgat ggtcgctacc ggttttagaa tgatcaacgt gaaaagttcc
    14401 cagtagctga catgatacct gcaatgatcg ttatcatgcc actgagctgg atgtggacaa
    14461 attggattat gccactgtta taataaaaat caaagagaag ccattttttg ctgactaaaa
    14521 aggatcaaat cttttagtca gtttttttgt atttgacaaa ctgattttga aactatatta
    14581 tatgttgtat aatatagatt gaaataaaat attacacttg agaatagaag gtgacgtaat
    14641 gcacatccag attccaactg ttcttcttga tggtactgtg ctagcagcat taagcaagga
    14701 agatatgtat gggtatgcct taacaaaaat cgtacaagaa agtctctctg tcagtgaatc
    14761 tacactatat ccggttttga gaagattgaa aaaagaagga tatcttgaga cctatgatga
    14821 accttttgaa ggcagaaacc gtcgatatta ctcgttgaca ccaatgggaa gtcgtcattt
    14881 agaagaaatt attgatgaat ggattgtttt tcgagattct ttagaccggt tattggagga
    14941 gagcacaaat gaatgaatat ttgaatgaag ttgtggatca tcttcaagaa attcctgaag
    15001 acgatcgttt tgatttgatc cagtattatg aggaatattt tttagattct ggaaaaacat
    15061 tagatcagat cattgatgaa tatggtacac caaagcagtt tgccttaaaa ttaaaaatca
    15121 gttacttttc agaagaagaa caagcagaaa acgagttagt gaaaaatcca aaaagacaaa
    15181 ttcgcctgat ttggttaatc attatcggtt tatttgcttc tcctgtcctc attccactag
    15241 cactggggtt tatattggtt attgggggac tattggttgc tttactagcg ctcattttta
    15301 gtatctatgt tttatgtatc agtatcttag gtgttggcct attcatgatc atctcaggaa
    15361 tcagtgtgtt tggacaatcg attctgagtg gattattctt tatcggtgtc ggtctgttcg
    15421 ccacaggaag cgcagtattt atttcgccac tgttgctgaa aggaacaaaa gggctttttt
    15481 atgtcatgat gaactttgtt aaatggatgg gacgacgttt tattacaaaa agagatctgc
    15541 attcagcggg cgtttgaagg aggacacata aattgagatt gaaacatttt ttgattaccg
    15601 gtattggtct gatgcttact ggtggcctcc tagcaggtgc aacttataca ttaggcgctc
    15661 aaaagagtct gacttgggat aatggtcctc ggttgatcga tatggttact gagacaaaaa
    15721 aaatcaagga tgcaaacaaa atcgtcatcc atgcagaaaa tcaaacagtt tcggtcatac
    15781 gcggattgga ttttgagata aaaacgactt atgataagac agaaaagcca gaaataaagg
    15841 agaaaaatgg cgtgttgact gtgaatgcag tcaaaaaatc aacaggtgca atcattagtt
    15901 tagaagacac aagtgcacaa atcactattg ttgtccctgc agatcatgaa ttatctgaat
    15961 tgacaatcga agggaataat agtgcaattg atatcaacga agtatcaatc aaaaaagtga
    16021 atactaagac acatgatggc tgggtatcgt tcaatgaatt tacaggagga acaataactg
    16081 cgaaagatga attaggctca cttcgattag atgaggccaa tgtggataac ttgatcgtca
    16141 caggtgaaaa cagcaacttc catctagaag acagtttgat cagcaaagaa ggaaaagtca
    16201 tcctaaacgg tggcaattta tctttgcatg agatggaaaa cagtggctat actatccaaa
    16261 cgtctggtga atcttatatt gaaaaagact accagccaat aacaaatact ttgttagaac
    16321 aaggacagaa aaagctaaag atcactgcta aagacacatc gatcaatatt acgacggatc
    16381 aagaggacaa tgaaacagat tgatcctgta ctcaactgga tattttaaga aaaaacagca
    16441 aagatccttc caatctttgc tgtttttcat cgcttaacgc acataactat agtaaaataa
    16501 gaaagaaaat atctaaaaag aaaacagggt gaaagagtat atgaaggtag aagaaggtca
    16561 aagagaaaag ttaaaaacag aaatggaaag attacatact tatatcactc aactaagcca
    16621 aaccttctat gatccagaca aagaaaaagt gatggttaat tacccgaaca attcagaagg
    16681 tcgccaatta gagcaagtgt accatgaagt tttcaaacat ttattgacgg tgaagaaaga
    16741 gctggattat tattctttac cgattatcga tacaggaata ttgaagtatg atgggaagaa
    16801 ggaacgattt atctttaaat ctgttcggga agatttgcct ctttcagcag ggatggatct
    16861 agaggtcttg gtagaagact attttacaga agaaaagcac tgggttcgaa caaaattgga
    16921 ttatttaccg caggcagccg gagggacaca ggcaagcggt tggtacatca ctgaagacaa
    16981 agagttagaa ctagaaggtg taatggcgcg tattcgaaaa aaaaattaaa aaagtaacat
    17041 tagatattta ttgaaggata aaggagcgca ggaagtagca tgagagaaaa aagagcattt
    17101 tatatcaatg gttatgcagg gattgtcgga ttggtcgtat tagctttagt tggattgttt
    17161 ttcttctatt taggtatgtg gcaagcgaaa gtttgggcac tttttttaag tatttttctt
    17221 tggttgatca cgttattgtt gttaagttcg gcaactgtag tcagtcctaa tcaagcaaaa
    17281 gtaattttgt tttttggaca atatttaggt acgattcgag aaaatggctt tttccttacg
    17341 atacctttgg cacaaaaaat gactgtttcg ttgaaagttc ggaatttcaa tagttctgtt
    17401 ttaaaagtca atgatttgga tggaaacccc atcgaaatct cagcagtagt agtctttaaa
    17461 gtcgttgata cagcaaaggc attattcgat gtagcgtatt atcaagattt tgtagaaatc
    17521 caaagtgaga cagccattcg gcatatcgct agccaatacc cgtatgatac attcaatgag
    17581 aatgatttga cgcttcgagg aaacacgacg gcagtgtctg acgagttgca aaaagagctg
    17641 caagagcgtt tagcagtagc aggtgtcgaa gtaatcgaaa cacgcttgaa ccatttagcc
    17701 tatgcaacag aaatcgctag tgccatgctc caacgtcaac aagcaaaagc cattttgtct
    17761 gctcgacaaa ccattgttga aggagctgtc acaatcactc aaatggcctt agagcaaatc
    17821 gaagatggac aagaaatcaa tttcaccgat gatcggaaag tccaattgat caataattta
    17881 cttgtctcaa tcattacaga caaaggaacg caacctgtta tcaatacagg cgacatgaaa
    17941 gaccgtaagt gacatacttt ttgtaagata ggaagaagga gtttaggacg gattattgtc
    18001 ttgaactctt tttttagttg ttttaaagaa aatgacaaaa ttgaaagaag aatgatagtt
    18061 gattcgaagg cagaattgtt cgcttttttc tataatcagt atataaagta agagagaagg
    18121 ggaaaaagtg aaaaaacttc tgatcggtct acttgttttc atcgttgtgg tacttgcagg
    18181 ggtgaaaatc gcagattacg tagttatggg tggagaagaa tactatgtac aaattacgac
    18241 agatggtaca aaaacagttt caaaatcaga ctctggacaa gaaacagtca actatagata
    18301 tatcctgcct ggctatacga aagagggtaa ggaaaaagaa ctggacttca agggacaatt
    18361 gactcgaccg cttcgtaaag gagcatattt aaaaatcacc tggaacaaaa ataaaggtgt
    18421 cacttcgtat gaagaagtcg aaaaaaaaga cgtgccgaaa acagcactgg aaaaactaat
    18481 taaagggtag ggaaagaaat gaacaaaata gtcgaagtac agaatgtaac aaaagtatac
    18541 ggaaaaaaca atgagaaaaa gacgcaagca ttaagcggca tcagcttcga tgtagaaaaa
    18601 ggcgaattta ttgggatcat gggcgcttct ggttctggta agtcgacact tttgaatatt
    18661 ttatccacat tagataaacc aacagatggg catatccgaa tcaatcaaaa agatgtgacc
    18721 acattaaaag gaaatcaact agcagatttt cgtgcgaatg agattggctt tattttccaa
    18781 gatttcaatt tattagaaag cttgacagca caagaaaata tcgctgttcc tctttcttta
    18841 caaggcgtca gaccaaaaga aatcaagcaa cgtgtccaaa aaattgcaga acgtttatcc
    18901 atctctcata ttttggaaag ctatccttct gagatttccg gtggacaaaa acaacgggtc
    18961 gccgccgcac gtgcattgat cacacaacca acgattctat taggggatga gcctacaggt
    19021 gcgctggatt ccaaaagtgc ccgggatctt ttagatacga tggacgaatt gaatacaaaa
    19081 gatcatgtat cgattcttct tgtgacgcat gatccgtttt ctgcaagtta ttgccaacgt
    19141 attttattca tcaaggatgt cggtatccat caagaagtga aacgtggaga tcaaagccgt
    19201 gattccttct atcgagaaat actatctatt cttggaaact tggaacagta gggggagatt
    19261 gactatgctt tggaaattat cacttactgg gatcaagagc cgtttaagag actacttggt
    19321 gttattctca ggattagtta tggcatctgc tattttttac atgtttgaat caatggcaag
    19381 caacgaagct tttttgaaaa gtaatacgat cgttgcggct acagttatca ttttccgatt
    19441 cggttctgtt ttactaggga tcatcacatt cgtttatatt ttatatgcaa attccttttt
    19501 gatgacgatg cggcaaaaag actatgcaat gtttatgatg ctgggagcaa aaggcagaaa
    19561 aatcgcacag atgatttttt ctgaaacatt cttagtaggg atcgcagcta ctttagtggg
    19621 ttcagctgtc ggtgtaggat tgacaagtat cgtccaccga ttattagtgg atcagttaga
    19681 tctcaatatt tcgcatttta caccttttag tattcacggg ttgttgatca ctgttttgtt
    19741 ttttgctgtt ttgtttttat tagctgctgt tgtcaatgct ttttcgattg tcaaaaaacc
    19801 gattttagca ttgattcgtg cggatcagac accaacaaga atcaagcagc ataaattctt
    19861 gtttttcctt gaagtagtac tgggaattgc ttttctaggc tgcggttact atatgatgaa
    19921 acacgtgggg acctatcaag tctttggtat aggggtagcc ttagttacga tcgtattagg
    19981 aacttatttc attttccatt ctgtgatcat tttcttttta tcattactaa agaaatcaga
    20041 acagatttca ttgaaaaaat tgaacaattt cacattgtca cagttaaact ttcgtattcg
    20101 tgaatataca caaatgctct caatggtcgc tatgctgttt gccttggcac tgggcgcttt
    20161 gacggtagga ttaggtttta aaaatgaaat tgcagacttg acagaaaaca attctggata
    20221 tgatctagtg atgaataatc ctcgggcaga agatcagcaa aaaatcaaag aactttcacc
    20281 aacactttct gcttcttata ctcaaaaaga agatgcggga acgatttact ataatgcaag
    20341 tgaatttgcg gctcaaccat tgatcatgat aaaacatgaa gatggaacac cgccgaagat
    20401 cacgaaagaa aaagttcctg ctaaaaaaat gaatgaactg actgtacaag aggaacttcg
    20461 aagctatgaa ttgccggaac agcaagaaaa agaaatcaag gtagtcagtc aatcagaatt
    20521 tgatcaactg aacttgccac aatccacttt acagctagtt caggtaaaag acttccaagc
    20581 aaacctgaaa ccaatcgaaa cacttgttgc tcaaaataaa atgaacaatc catctttgca
    20641 aaaagtagag tacagcaacc agaaatatga aatgtatgaa gtgtataatg gtctattctc
    20701 tggttttgaa tttatgggat tcttcctagg tttagctttc ttgaccatgc ttgctagctg
    20761 tttgatgttc aagatcttgt caggaagcaa gagtgacatt gctcgttatg aaatgcttga
    20821 aaagatagga acacgacgcg ggttgcttaa acaatcgatc cgtagagaag taggcgtgtt
    20881 attcttagca ccaggaatct taggtgtagt ccatgtctta ttcggattac aaatgtttgg
    20941 tctattcatg caaaatccat acaaagatat ttgggttccg tttacgatat tcttcgtatt
    21001 gtatttcatc tactatgtac tgacgacatg gttatatact ggaatcgtat tgaaaaagag
    21061 attgtgaaaa agctgtcctg catcaagtaa taagaacagc caagcaaaaa tagctcctca
    21121 tatttttcgt ttggttgggc ttattaccgc agatgcaagc ttttgaacac cgtttgttcg
    21181 gaaaaagagg ttgtgacaat atttttgtca caaccttttt ttcacggtct tttattagaa
    21241 tatagtaaat tttttctcgt tactcacgga ataagctgtt aagaaaacgg tttccatgta
    21301 tactggaatc agtgcaaatg aaagaaaaga gggtgcaagg aacatggaaa tcaatgaaca
    21361 aaattttggc gaaaactatc acttgattac gatcacgaat caaaatggga catctttgtc
    21421 tgtcagtgat cttggggcac gggttgttca ttttcaaaca ataatcgata atgaaaaacg
    21481 agagttgatc ttaggttttg atgaagcaaa agaatatcta gaaaaagacc cttatatcgg
    21541 tgcttcaatc ggacgtatgg ctggacggat tacgaatggc acattcaaat tagatgatga
    21601 aacgttcgaa gtaaaaacag acccggacac tggtcattgt ttacatggtg gtgcacctag
    21661 ctttgaagcc aagcagtggc attatacagt catcaatggt gaaaacgaag catcggtcat
    21721 tttctatcag actagcccag atatggaaaa cggatttcct ggaactttag atgtagaagt
    21781 acggtatacg ctgacgaacg atgatacatg gcgagtgacg gtccaaggga tcagtgaccg
    21841 caaaacagta ttcaatccta caaatcatgt ctatttcaat ttaaccggtg atccagcaca
    21901 gtcaatcgac gaacatatac tttggttgaa tagccgatta tttgctacgc tcaataaaga
    21961 tacaacgcct acaggagaag tagtttcagt aagtggcaca gcatttgact tccaacgtcc
    22021 taaaaaatta ggtgaggtct ttgccagtga ttttcgccaa tcaatgatag tgggcggcat
    22081 cgatcatcca tttttgttga aaaaacaatc tggtttacta aatgctgcgg aattgacaag
    22141 cccagatgaa aaagtatcga tccaagtaaa aacagatgct tcaggaatcg tgatttttac
    22201 agcaaatttt ggagaagatg gaccggaaat gcgcgggaat aaattagtaa atcatggcgg
    22261 tatcacattt gagactcaag aactgccagg agcggagcga cttgaaagtt ttggagatgt
    22321 cactctggaa cctaaccaag tacgcgagtt tgtaacggaa tacaaaataa atataaagaa
    22381 atagaataaa ttaaaaaggg agtgtgacag tgtcacatcc cctttttaat ttattctatt
    22441 tctttaaagg cagatcaagc cagaatgatt tgtctaaact taacaaggtt agtacaggaa
    22501 cagttgcagc tttaactgta cgagtagttg tttctggtat ctgtcttgcc gtaaaattgt
    22561 tccacaaatc ccagattgaa gcgacacgga agatggaaga agcgttgttg acgcgtttta
    22621 tcgttgaaaa ttctaatcct gtgattggca cgccatctgt tgtccatttg attggcacat
    22681 cgaatcgctt agacatattg aggatttcag cgtttgcttc ttcagttgtt gctgcttctt
    22741 ttttcggttc atcatatttt gtcaggtcgt acgcttcgat gatcgcgttc aatttttgtg
    22801 aatattggcg atctgtagca taacgtcctt ccaaatattt ggttgcttct ttgtagtctt
    22861 ttgtttccga cttccaagtt ggtttgtaga aatcaggatt gccagtcagt ccatttttga
    22921 tcaagtcggc atagtcttcc aaactttcct tgtaagaagg atatttacgg aattgtgcgc
    22981 ggatttggta gctttgtccg gaactatctt gttcaagtgt attgaaggtc acgctgcttc
    23041 ctttatatga tcctttgata ccaaaaaggt tatgatttgg ttcgcttgat aataagctgt
    23101 ttccagatcc actttcaagg atagcttgag ccatcatgac tgatgcgtag attccttctt
    23161 tttgcccgat ttcctgtgca tcatctgcaa tctttttcaa gaactgatca gttgtttggt
    23221 tttgactgaa atggaacgag ccatctaatc catctaaaat agatgagccg cctgtcagcg
    23281 aacctgaagc agcaggtaaa cctgctaagt ttactcggac agcaaaatca ggcgtgaaat
    23341 ctgaaataga agttactcga actttatctc ctggttgagg cgcatggata aattgtccgt
    23401 tcccaatata gatacctaca tggtgagtaa caccggaaga aggtccccag aagacgaggt
    23461 cgccaggttg ggcctgactt actggaatct gcgtacctgc atattgctgt tcgcctgtcc
    23521 agccaccgat ataatgaccg gttgctttca tatatacgta ataagtcaaa ccagaacagt
    23581 caaaagtatt tggacctttt gctccccata catatggttt tccaagatgc ttgaatgctt
    23641 ctcgaacgat tgcttgttgg acattcgttg tatttgtagg caatacagca tcaattggtg
    23701 attttacaac aaaatcagaa gaagcagaag atggattctc tataccgctt tgatttgctg
    23761 tatttggacg agatgaagca ttgttcgttc cgccattcga tgaaccattg tttggatggt
    23821 ttggttctct ctgagtattt ccattgttat tcgatccaga tgggtttgtt tgattcgaag
    23881 aattcccgct gttcgaatta ttgttattat tgtttgaatt atttgaacca ttattattgt
    23941 tgttattacc cacatctggt ttgtttggcg tttccggatc tggtgtacta gggacttctt
    24001 cactaggatc agttggtttc tctgttgaag tatccggtcc agctttaaat tctgcagtaa
    24061 tagataaatt gttcgtacct actgtaaagg tctttgcatc ataatttact tctttggcac
    24121 cattaacagc gactgaagca atttgttcac cttcagctgg cgtaatagtg taagttacct
    24181 tcgtttccgc taataaacct tctaccgttc cactttccgt aatatccaaa ggttgctgtt
    24241 caggggaatc cgctgtcgaa gcagtcagtt ggatcttacc aatcaaagaa ggagcaattg
    24301 taagggaata tgtctcggct ggcttttcat tttctgatgg atcagccgga tcaatttcag
    24361 ttggctcggt cgatcctgtt gttgagttag ttgttccagt tgggtcttct gtaaaagaat
    24421 ccgttgtttc actagtagct gagctggatg tagtcgtatc tgttgtgact gtttgatcag
    24481 ctatttgatt atctacggca aatacggtat tggtcgacaa aacagacgga gcaatcagcc
    24541 aagtagcagc tagcaaacgt atcgtttttt tatttttcat aataaactag tccttttcct
    24601 ttcattgatt ttctgtctta ttcagcattc attagacatt ttttggaaca gataatcgtg
    24661 tcgaaaagct ttttcttttc ataaatctgt ctgtgtattc taaataaaac aatttgtaaa
    24721 taagcaacga gcattcattc gtttttcttt gacgttcatt ttattgcata gaccaccttt
    24781 gtttggggat aaacaagtgg ggataaaaat ggttatgcat atgtaaatta taccaaagtt
    24841 tggaaagtat cacaaaaatt tataaaaata atgatcctaa aaagaaaaag actgaaatat
    24901 gtgataaaca tctcatactt cagtcttttt cattaactat tcataagaca ttaggtcttc
    24961 atcaaacccg ctaacgagag tagtgatcat tgctacgact agactaacag ccagtcctgc
    25021 taagtaccag aaaaaagtac cggaaatgaa tgcaggcaat gtggtcagac taccaaatac
    25081 aaaagcgatc gctttcactt gggcaaatcc cattacagct ccaccagcag ctgcaccaat
    25141 gcaagcagca acaaatggtt ttttcaatgg gatcgcaatt ccgtaaacag ttggttcggt
    25201 cactcccgca agtaaaatcg aagcaaaagc agaaccggag atcgaacggg tttttgaacg
    25261 tttacttcgc agaaatacac ctaaagctgc accagccatt ccaaaattgg ctgctcctgt
    25321 caacggaacg attcggtcga acccataagc agagatatta ctgatcatga cagggtttac
    25381 agcccattgg ataccaaata ttaccaaaat attccaacca ccagcgatga ttgcgccact
    25441 gatcggtccg ttaatatcca tcatccaagt aattgcggca gctaaacctt cgccaaggta
    25501 aacacccgca ggcccgaata ccattaacgt caaaggaacc gtgataacta atgaaagtaa
    25561 aggtacaaat actaattgca tattttcgcg aatatatttt ttgaggaatt tttcaacaaa
    25621 agaataaaag aaaataccta aaatcgccgg catgacagta gacgtatact gcatcagtac
    25681 tacaggaatc cccagaaaat cagtagctgc tttgccactg tctgaaagta aagaggtcag
    25741 attcggttca atcagtgctg cagcaatgac agccgcaata tatccattca ctttaaacgt
    25801 tttggcagca gaaaaagcaa gaaagattgg cagaaaataa aagacggcat tcgacgcagc
    25861 agacaaaata tgataagttc cgcttgtttc actaagccag ccaatcgtag tcgctaaaac
    25921 aaccagacct tttaatatac cagaaccgtc aaacagcgga atgatcggcg taaagatttc
    25981 cgatatcaaa ccaaatagtt tttcgatcgg atttagcttt tctttcggag ctgaatgatc
    26041 gatttgacgc gtcaattcct tgtaagcagg agccaatccc tgatccatga ttatttctgc
    26101 atatctatcg agtaaaatga aatacttaat ggaagggatt tgctgtttta atttttctgc
    26161 attggctact ggctgttttt tcaatttgat acgaagccga gtcgcacatg gaaggacaga
    26221 atcaatattg tcttttcctc ccaactgttc aatcagcata tgaatctgct cggacagtac
    26281 catctgttca ggattcattg tcgtttccat tagaagacca ctccactcac taatataata
    26341 ttggcgtaag gtgtcatttc gcctgtacga ataaatgcat gacagttatt caactctttt
    26401 ttcatttctt catgtgggat aaagctaatc ggtgtatctg gtaacaataa tttgattttt
    26461 tccaatattt ccgggttcat tgtctttatt tcttccgcca attcaatcga ttgtacctca
    26521 agttcttcta aaacgttttc taaaacagat aaaaaacttg gaagaccgtt tgagacagcc
    26581 aaatctattt tttcagtacc aaaggggaca ggcatgcctg catccccgat acttaattta
    26641 tcaaagtgcc ccatttgtgc gattactcgt gaaatatctg aattgattac ttttcctttt
    26701 ttcatgtttg tttcttcctc tcattgttaa ttttagactg ctgattgttc taaagcttcg
    26761 cggtaaggga tagaaggttg cgcaccataa gactgcacag caacagaaga ggctagattt
    26821 ccataaagga tcgcttcttt caagttacta aggtctggct gaagtgtgct gcttaatgcg
    26881 ccaataaatg tatcacctgc ggctgttgtg tcaacggcat ccactttaaa agcagggacg
    26941 ataccgtgtt ctttttctgt atgatagaaa gcgccggcac tgcctagtgt aatgattact
    27001 gttccaatac ctaattcatg gagtttttcg gctgctgcga ccaaactgtt atgatctgtc
    27061 actctgattc ctgtaatgat ttctgtttct gtttcatttg gtataatgat atctgtcttt
    27121 tcaagtagtt cagtaggaat cgtttcacga gctggtgcag gattcaggat cgttgttttt
    27181 cctgcgtctt ttgcaataga aaaagctagg atcgtcgcat ctaagctact ttcaaactga
    27241 gcaataataa agtcactgtt tgtaatcagt tgcttgtttt ttaggacttc ttgttcatgg
    27301 aaagcattat ttgcccctgc atggatcaag atactatttt ctcctgctgc gtctaccata
    27361 atcattgctt gtccggttgt ggcgccttgg atttcagcta tcccagatgt atcgatgttt
    27421 tcttttgtca gtagatctaa taactgttgt ccttcgctgt cagccccaac gcctccaata
    27481 aaagatgtcc gtgcgcctga acgtttggct gccactgctt ggttggcacc tttacctccg
    27541 ccagcgtgaa aaatctcatg cgcatgcatc gtttcgcctg gcttgggcat gttggttaaa
    27601 cgaagggtag tgtccatatt aatacttcca attactgtaa ccgtgttcat tttacaaacc
    27661 tccaactatc tcatttattt tgatgggaaa attatttttt aaaacgtttt agtaaaacgt
    27721 tttaatagtt cgataaaaat atagcacacc ttgaaagcta tttcaagggt gcagttgaaa
    27781 atcttttttc taatcgtaca ggtaatcttt tttcttccca ttctttttct gggaattgga
    27841 tccgatctaa caataacttg gcagatgttt gtcccaattc gaagattggt tgtgcgatcg
    27901 ttgtcagttt cggcttgata tactcgcaca tatctatatt atcatagcca ataatgctgt
    27961 agtcttcagg gatgcttttt ccagcttcct ctaatccgcg atataatcca aaagctaatt
    28021 catcatttaa agcaaacaca ccagttgcag cagaatcaag aagttctgct gtgatctggt
    28081 agccaccttg ttttgagaac tgtgtaggta agaggatcag ttggtcgtga gggatttggt
    28141 aaacatcaag agcagattta aacccctcaa tccgagcgtg gacattttcc ggtggatttt
    28201 caggatagac aagggcgatc gtttgatgtc ctagactaag caagtgcatg cccgctaggt
    28261 aaccaccgcg aaaatcgtct gttcgtacgg catcactaaa tccttcagat tttttctgat
    28321 ccaaaacaat gaagggccgg ccttgttttt tcaaattttc gttgattgca tctgtagaga
    28381 cagcagaagt cgcgataatg aatccatcca cacctcgacg tgtgagctcg gctagatatt
    28441 cgatttcttt ttgatgatca gaatcagcat tacataatat cgtgacgaaa ttctgcttat
    28501 acaagatatc ctcaattcct cgcatcagtg tactgaagaa tggattcgtg atatcaggaa
    28561 cgagtacacc aattgttttc gtttccttgg taatcatttg tctagcaaaa tagtcaggct
    28621 gatagcctaa ttcttctttg gcagctaaaa ctttctgaac agttttcgga ctgaatttct
    28681 ccgtatttcc atttaatatc aaggagacag ttgtgattga aacgccggaa agtttagcga
    28741 cttctttgat tgtgatcttt tttttcacca tgttcatact cctatctaaa tcctgtggtt
    28801 attttcccat gaaatcaaaa agaagtaaat gaaaatcacg tgtgccagta cttatttgct
    28861 ttgttggata gcttcgatta tttgtttcgt tgagcgtact cgtgcagttt tcggaaatat
    28921 cttttttatg aacaattgat gggattcttt gtcacgatca ctgcaagcat cttcagcaag
    28981 cacgatatgg tacccgtgtt gaaaggcatc taatgcagta gcatatacac cgtttgttgt
    29041 cgctacaccg cttaaaatga tagtttcgat tccacgccgg cgaagttgaa gatcaagaga
    29101 agtaccaaaa aaagcacttg gattgtattt tgtgatagtc aacatattat ctgtatcttt
    29161 caaagaatcc ttcaaaagta aatcagtgaa ttctggaggt acagaagcag tcattccacc
    29221 catatcagtt tgttgactca aataagggaa agtggagatg tctacgttca ctaaaacttt
    29281 caaggcagcg gtacgtttca atgttcttac taacaaatca ttgtttgtca gaatagatgc
    29341 tgcagtgtgt ggagagagtg taccgttatt tacgatctct ttttgcatgt cgatcgcgac
    29401 aaaagcagtt tttgataaat caaaaatcat attttccttt aaatcattca tgatgttcct
    29461 tctttcaaat aacaatgttt tttatgtaag taaatcataa atcgtttatt tgtcaactaa
    29521 attaaaggat tcctttcttt agtctagaga aattcagtgg taaaataaat aagtgtgaga
    29581 aaacgcattc gatgcataga tatagcgaga aatatcgtga aaatggagga atcagagtga
    29641 agtatgtcga agtaaaaaat gaatacaaac gatacaaaat gggagaaacg accattacag
    29701 caaatgacga tatttctttt tcaattgaaa aaggcgaatt agtgattata cttggtccaa
    29761 gtggagcagg aaagtcaacg attcttaata ttttaggtgg gatggataca cctgatgaag
    29821 gtcagatcat tattgatgac acagatattg cgcaattttc agataaacag cttactgctt
    29881 accgacgaac agatgtaggg ttcgttttcc aattctacaa tctcgttcct aatctaacag
    29941 caaaagaaaa tgtggaattg gcaacggaag tttctcctga tgcgcttgat cctgtagagg
    30001 tattgaggca agtgggccta gctcatcgat tgaataactt tccgtcccaa ttatcaggag
    30061 gcgaacagca gagggtttca attgctcgtg cactagctaa aaatcccaaa cttcttttat
    30121 gtgatgaacc gacaggcgcg ctggattttg aaacgggcaa gcaagtattg aaattattgc
    30181 aaaacgctag cagacaacac ggcaataccg ttttgatcat tacacataat tctgctttag
    30241 cgcccatcgc tgatagagtc attcacatta atgatgctaa agtaaggtct gttgaattaa
    30301 atgatcatcc ttcatccatt gatgagattg tatggtaggg ggattttatg aaaacaagaa
    30361 catatcttaa agcaggtttt cgcgaaattc ggcaatctaa aggacgattc attgcaatca
    30421 tccttattgt catgctagga acacttttat ttgtaggtgt caaaactgct ggacctgtca
    30481 tgcaaaaaac gatggatcac tatgttggta cagcaggact ttctgatttg caaatcgttt
    30541 ctactggtgg cttgacacaa aaagatattg ctcaagcaga aaagatccca gatgcaaaag
    30601 tagaaacagg aaaacagatc tattattcca atactggcaa aaacgaagtg attcaaatat
    30661 tttcttataa caaaaataac aagcagaaca agctacaatt aactgatgga aaactcccgg
    30721 aaaaaccaaa ccagcttgta ttggatgaaa aagcggaaga tgaagggtat aaaatcggtg
    30781 atatttatca gattgattca gatgaattga aagaaaaaga gtataccatc acgggctttg
    30841 tccgttctcc attgttcatc aacaatttgg agagaggata cgcgaacgtc ggaaatggta
    30901 gcgtcgatta ttttgtctat ttacctgaat ccgtttttaa aacagatatt tattctgtct
    30961 tatatcttga ttttacaaac gtaaagaatt tgaatacgta cagcaaagct tacaaagata
    31021 aaatggagca aaatcaagaa aaggcagaaa agtatttaca agatcgaccc gaagaacgct
    31081 tagaagagct gaaaaaatcg gccaacgaag aattagaacc ggcacagcaa aaaatagcgg
    31141 atggcaaagc acaaacaacg caagcaagaa cacggttaga agatgctaaa aaacagttag
    31201 cgcaacagtc agcaactatc cagcagcttc ctgaagcgca acgaacaatc gctgttcaaa
    31261 cattatctca acaagaagct caacttgctg aacaagaaag gcaattagtt gagaaagaac
    31321 gtgaactggc ctctgcagag gaaggattga atcgaaatgc agaaaaaatt gaaaatttaa
    31381 ctaaaccatc atacctatac aatgaacgtt cagaaaatgt cggttttcaa gaatttggtg
    31441 atctgtctga tcgtatcgct gcgattgcca atgtttttcc tgttttcttt ttctttattg
    31501 ccgcattgat cacctttacg acaatgacac gtatggtaga agaaaaccgc agggaaatcg
    31561 ggacattaaa ggcgttaggt tatacgaaag ttgaaatcgc aaaaaaatat gcaatctatg
    31621 caagtctagc ctcagggata ggtattgttt taggaacgat attaggaacg aatcttttgc
    31681 caaggatcat ttttgagctg tctaacgagc ggtatgatat tgggagtgcg cttatttttt
    31741 atgactggcc gccgatttta caagcagctg ccgcattctt cattgccgca ttt
//
LOCUS       DACEXQ010000007        31362 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.007, whole genome shotgun sequence.
ACCESSION   DACEXQ010000007 DACEXQ010000000
VERSION     DACEXQ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.007"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            95..1705
                     /locus_tag="IXH04_000248"
     CDS             95..1705
                     /locus_tag="IXH04_000248"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAQ1850883.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            1997..2866
                     /locus_tag="IXH04_000249"
     CDS             1997..2866
                     /locus_tag="IXH04_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAQ1850884.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            3031..4302
                     /locus_tag="IXH04_000250"
     CDS             3031..4302
                     /locus_tag="IXH04_000250"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ1850885.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            4299..5594
                     /locus_tag="IXH04_000251"
     CDS             4299..5594
                     /locus_tag="IXH04_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAQ1850886.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            5693..5956
                     /locus_tag="IXH04_000252"
     CDS             5693..5956
                     /locus_tag="IXH04_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAQ1850887.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            6401..6850
                     /locus_tag="IXH04_000253"
     CDS             6401..6850
                     /locus_tag="IXH04_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1850888.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            6907..8325
                     /locus_tag="IXH04_000254"
     CDS             6907..8325
                     /locus_tag="IXH04_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1850889.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            8325..9185
                     /locus_tag="IXH04_000255"
     CDS             8325..9185
                     /locus_tag="IXH04_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAQ1850890.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            9359..10132
                     /locus_tag="IXH04_000256"
     CDS             9359..10132
                     /locus_tag="IXH04_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ1850891.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            10279..10644
                     /locus_tag="IXH04_000257"
     CDS             10279..10644
                     /locus_tag="IXH04_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAQ1850892.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(10792..12000)
                     /locus_tag="IXH04_000258"
     CDS             complement(10792..12000)
                     /locus_tag="IXH04_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1850893.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(12019..12297)
                     /locus_tag="IXH04_000259"
     CDS             complement(12019..12297)
                     /locus_tag="IXH04_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1850894.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            12490..13146
                     /locus_tag="IXH04_000260"
     CDS             12490..13146
                     /locus_tag="IXH04_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ1850895.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(13588..14385)
                     /locus_tag="IXH04_000261"
     CDS             complement(13588..14385)
                     /locus_tag="IXH04_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAQ1850896.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(14382..15566)
                     /locus_tag="IXH04_000262"
     CDS             complement(14382..15566)
                     /locus_tag="IXH04_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ1850897.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(15591..16838)
                     /locus_tag="IXH04_000263"
     CDS             complement(15591..16838)
                     /locus_tag="IXH04_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAQ1850898.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            17053..18798
                     /gene="dnaX"
                     /locus_tag="IXH04_000264"
     CDS             17053..18798
                     /gene="dnaX"
                     /locus_tag="IXH04_000264"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAQ1850899.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSSIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            18818..19132
                     /locus_tag="IXH04_000265"
     CDS             18818..19132
                     /locus_tag="IXH04_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAQ1850900.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            19157..19753
                     /gene="recR"
                     /locus_tag="IXH04_000266"
     CDS             19157..19753
                     /gene="recR"
                     /locus_tag="IXH04_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAQ1850901.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            19817..20461
                     /locus_tag="IXH04_000267"
     CDS             19817..20461
                     /locus_tag="IXH04_000267"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAQ1850902.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            20476..20805
                     /locus_tag="IXH04_000268"
     CDS             20476..20805
                     /locus_tag="IXH04_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1850903.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            20819..21757
                     /gene="holB"
                     /locus_tag="IXH04_000269"
     CDS             20819..21757
                     /gene="holB"
                     /locus_tag="IXH04_000269"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAQ1850904.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            21793..22617
                     /locus_tag="IXH04_000270"
     CDS             21793..22617
                     /locus_tag="IXH04_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAQ1850905.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            22610..22957
                     /locus_tag="IXH04_000271"
     CDS             22610..22957
                     /locus_tag="IXH04_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAQ1850906.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            23026..23898
                     /gene="rsmI"
                     /locus_tag="IXH04_000272"
     CDS             23026..23898
                     /gene="rsmI"
                     /locus_tag="IXH04_000272"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAQ1850907.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            24007..25128
                     /gene="dinB"
                     /locus_tag="IXH04_000273"
     CDS             24007..25128
                     /gene="dinB"
                     /locus_tag="IXH04_000273"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAQ1850908.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(25182..25784)
                     /gene="nrdG"
                     /locus_tag="IXH04_000274"
     CDS             complement(25182..25784)
                     /gene="nrdG"
                     /locus_tag="IXH04_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAQ1850909.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(26099..28288)
                     /gene="nrdD"
                     /locus_tag="IXH04_000275"
     CDS             complement(26099..28288)
                     /gene="nrdD"
                     /locus_tag="IXH04_000275"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAQ1850910.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            28614..29381
                     /locus_tag="IXH04_000276"
     CDS             28614..29381
                     /locus_tag="IXH04_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1850911.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            29371..>31362
                     /locus_tag="IXH04_000277"
     CDS             29371..>31362
                     /locus_tag="IXH04_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1850912.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYL"
BASE COUNT        10076 a         5622 c         6582 g         9082 t
ORIGIN      
        1 aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc tgagactttt
       61 cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt ttgtgaccgg
      121 cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac gcttattgaa
      181 aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg tggatccggg
      241 gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg ctgaaacaga
      301 tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat actccaacgt
      361 gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg aatacttggg
      421 agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa tcatgcgagc
      481 agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag taggggatat
      541 agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg gtacagataa
      601 tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag aaatgaaaac
      661 taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac cgaatatttt
      721 ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg cacaattttg
      781 tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt actccatccc
      841 attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa aactagatac
      901 accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga acttgaagaa
      961 aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat atatctcagt
     1021 agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga tcgattggat
     1081 caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg cagatggaat
     1141 cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag cgattcgtta
     1201 tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga tggcttgtgt
     1261 cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa cgaatccaga
     1321 tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga atttgggtgg
     1381 aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga cggctgaagc
     1441 atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt tcaataacaa
     1501 atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac cagataaccg
     1561 tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc aattccaccc
     1621 agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg tcggagcatc
     1681 attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa agtagcaaga
     1741 gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta ttatttgtag
     1801 gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat attttatttt
     1861 ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa ctaccgaaat
     1921 gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt ttacaaaact
     1981 taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag ctgctcgtaa
     2041 aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc aagcaatcct
     2101 tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg gtgcagctaa
     2161 atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg aagcaatgaa
     2221 catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg ctttggaatg
     2281 tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat ttgaagaaaa
     2341 cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct cagttgaatg
     2401 tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg aattagctga
     2461 tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt gcggtatcgg
     2521 taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc acttacaagc
     2581 aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat caggtattcc
     2641 tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg ttaacactga
     2701 attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta aagataaaga
     2761 aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa tcgttgaaga
     2821 tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat ccacataaca
     2881 gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct tttttactta
     2941 aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag catatccatg
     3001 aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa aggaaaccga
     3061 cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc attgattcca
     3121 gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat acaagatgtt
     3181 cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc caacaacata
     3241 ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa aatcaatagt
     3301 ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga agcggtagta
     3361 ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt caaaggattt
     3421 gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg gacagaaaat
     3481 aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc aacgatcaat
     3541 gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc tgcacgagaa
     3601 ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt tcgaggagcg
     3661 ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg gcatttcatg
     3721 attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt aggaaatggt
     3781 atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa attgcaggaa
     3841 attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc gaaagaatta
     3901 aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct gcagcagcca
     3961 cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac gatctatgcg
     4021 aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac cgtggaagga
     4081 aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt ggcttctgat
     4141 cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac aacgacgatt
     4201 tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact aacagcctta
     4261 ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat ttgacaatgg
     4321 cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa tttaaaattc
     4381 cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt gcacaagcag
     4441 agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa gatggttacg
     4501 gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt tctgcctcac
     4561 aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc cgtccaccta
     4621 aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga aaagatccgg
     4681 aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg caaagacagc
     4741 ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc ttttctccaa
     4801 tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa acttctgtat
     4861 taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta atcgttctac
     4921 tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa ggcgacgtgg
     4981 tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa ttagtattgg
     5041 aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg gatagtatta
     5101 ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca ttgagtggcg
     5161 ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca agaaacatcg
     5221 aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga agccgtatgg
     5281 atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa ctttcacgtg
     5341 aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt acccgaaaag
     5401 aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga aaaaatatga
     5461 ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa acaaaaaaca
     5521 atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat ccgaccagaa
     5581 atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg ttgtaaatcg
     5641 acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa tcatgaaaga
     5701 aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg gtttcaaatt
     5761 cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg gtaatacata
     5821 cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg gacgtcaaaa
     5881 attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc tcaaagacgc
     5941 aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta tctgataggg
     6001 atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct gaaaaagtcg
     6061 taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta tccacttgat
     6121 aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa accatttacc
     6181 taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta tagacttgcg
     6241 attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga tgggtctttt
     6301 tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg acctcagttc
     6361 caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg atcaattgat
     6421 cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat tatttgagcg
     6481 aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag tcgttaaggc
     6541 actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg ggattcccca
     6601 tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag ccaaattaac
     6661 ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg tagttcctga
     6721 aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat tgatgaatgc
     6781 agattttaga aagggaataa aagaagccac tacgccagca gatatcatca gacagattag
     6841 tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa aaggaggaaa
     6901 ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca tacttatatg
     6961 tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa attcgaaaca
     7021 caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc agatgcaatc
     7081 attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa agtaaagcgt
     7141 gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc attgagagat
     7201 attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa aaagccgaca
     7261 atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat cgcaggcgga
     7321 atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa ctcagcagac
     7381 tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt tgccatgatg
     7441 gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg ttttgcccca
     7501 ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg cggaatgatt
     7561 ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag cgtaaaaaaa
     7621 gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt aattattggg
     7681 cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc tttgacaaat
     7741 tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat cgggatcatg
     7801 atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc gatcggtttg
     7861 atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat gacacctccc
     7921 ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga agaacggact
     7981 tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc tataccgttt
     8041 gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc agtcgctgca
     8101 gggatttcca tggcagtagg agttacttca atggcacctc atggagggat ttgggtgatg
     8161 ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc atcaggtaca
     8221 gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa aaaacaagac
     8281 aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta tatacaatga
     8341 aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc aatatttgca
     8401 gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca ccagtaatct
     8461 tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg gcagtaagag
     8521 cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc ggtacgatag
     8581 aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat gcgtctttga
     8641 aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct catgcggtaa
     8701 atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct gaaggaggtg
     8761 ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa gaaacaggaa
     8821 tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa aacagaactc
     8881 caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt ccatttgttt
     8941 tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa tacggagttg
     9001 ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg agagaaatat
     9061 tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct aataaaaagg
     9121 tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag gctagtttat
     9181 actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat accctcttaa
     9241 ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc tcgaagccaa
     9301 tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg gtgattttat
     9361 gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa acaaccaatt
     9421 tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga ttaaacgtga
     9481 ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg ccatgcttat
     9541 tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag aagaagaaac
     9601 agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata tggtcttatt
     9661 cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat ttgatggttt
     9721 gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata caacggcaaa
     9781 agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg gttcaaaagc
     9841 ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc gcgggctaga
     9901 tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa aagcttttag
     9961 aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac ataaattcat
    10021 gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag aactgacaaa
    10081 agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat aaattattac
    10141 gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc tcgcttctct
    10201 cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt ttttatccag
    10261 gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc aatcatgatc
    10321 tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat ggattcaacg
    10381 attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt gatcttacgt
    10441 aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt tatcttgtgg
    10501 ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt gattcctgct
    10561 ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca aggcatcgta
    10621 ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga taaatgaaag
    10681 ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta ctcaaaaaac
    10741 cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag atcaaatttc
    10801 atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc ggccattaac
    10861 aagatagagt gctgtacata tcaatggaat gactccggca atcacataca atcctctaaa
    10921 tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc ctaggtctaa
    10981 accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga tttttaaaca
    11041 aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg ctccagagat
    11101 aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg ttaagaataa
    11161 atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc cagtcaatgg
    11221 acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag cagcaactaa
    11281 gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag aaagacccat
    11341 caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat tgatattcca
    11401 tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag gaaagacaag
    11461 gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga aacgaaaatc
    11521 agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta aactagtact
    11581 taatccataa taattgatgc cttccccttt tttagatgca gggatatatg cagtaacgat
    11641 agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc gaactaaata
    11701 caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat agaagatcgc
    11761 gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca tgaataagcg
    11821 tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc tcaagctggc
    11881 gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat aaatcaggta
    11941 gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt tcttttccat
    12001 gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt gaacttctac
    12061 atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat ctcttgtaga
    12121 cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca ctgctttgat
    12181 caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt tcatatcaaa
    12241 tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt aattcatgtt
    12301 ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa aaaaggaaaa
    12361 aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta gtaaaatacc
    12421 tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag tttggatggg
    12481 ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga ttttccgatt
    12541 atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc ccatacgtat
    12601 atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag agaatttaac
    12661 cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata ttcacaattc
    12721 atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta tcgaatcaat
    12781 cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta tgtaaaagag
    12841 tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct attaaacgga
    12901 aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg tgtgcggaca
    12961 gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa attttgtggc
    13021 atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga tggtattatc
    13081 accgttaaag accatcatat cgttattttg aatatggact tgttagaaga caatattatc
    13141 ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag agaaacaaac
    13201 ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt cagctgattg
    13261 aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca aggaacaatt
    13321 tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg agttggtagg
    13381 agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa taagaagagc
    13441 caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt agatgtaagc
    13501 ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt cacagccctt
    13561 tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc caaaagtccc
    13621 gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg ggcttttggg
    13681 gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag ttaatttgat
    13741 actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat aacgcgaaac
    13801 agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa tgatgatttc
    13861 tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta cacgataatg
    13921 tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag cttttgcagc
    13981 attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg aatcgattct
    14041 gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt cacgctgtcc
    14101 catatctaat agcaatactg agccattttg atcatttccg actgcgtctc ttaagtaatt
    14161 atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca agactcgtcc
    14221 tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt gatctgttgt
    14281 gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca ccggccagcc
    14341 aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa gtaacctccg
    14401 atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc caattttttc
    14461 tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc gtagtagtag
    14521 acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag taatacgtaa
    14581 ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt tgtatatcca
    14641 tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg aatctgtgat
    14701 ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa atactggctt
    14761 ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat cttttgttgc
    14821 gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc tgctcttgaa
    14881 acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat gcggataact
    14941 gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata agggaaatac
    15001 acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac aaaccatttt
    15061 ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc aaaaggaagt
    15121 actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg attatggcag
    15181 tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc acagttggga
    15241 caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt atctgaaaaa
    15301 ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag ctgttcttgg
    15361 gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc gccagccata
    15421 cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata gatattcagg
    15481 cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc gccgcaatta
    15541 ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa ttaattaacg
    15601 gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc gttggaaaga
    15661 tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac gttttctaca
    15721 ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc gcaaactgga
    15781 catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc tgcttgttgt
    15841 ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt tgcattcatt
    15901 cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc ggctgcttcc
    15961 ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc acctagcatc
    16021 gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga aatgctggct
    16081 acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc caatgcagta
    16141 tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg ttgaccatct
    16201 gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa atactgctca
    16261 ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt ataaaataaa
    16321 atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa ttctgcgtta
    16381 tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc ctttatttct
    16441 tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa tttgaatcgg
    16501 tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg agctgcgtca
    16561 tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc accatcgacc
    16621 aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa aacatcttct
    16681 gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc ttttgtcatg
    16741 accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc tgccagaccg
    16801 ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc ctctgattca
    16861 gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga aacttctcgg
    16921 actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa aaagccgaga
    16981 aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat tttttaaaaa
    17041 agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca gcgtttcgaa
    17101 gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt ttcccacaaa
    17161 acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag tgcagcaaag
    17221 attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg caacgaatgc
    17281 gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat cgatgctgcc
    17341 agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta tgcgcctaca
    17401 aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac aggagctttc
    17461 aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat cttagcaacg
    17521 accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt tgattttaaa
    17581 cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga atcgactatc
    17641 agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg gatgcgggat
    17701 gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac tttagatgat
    17761 gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt gagtgcttgc
    17821 gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc agccggcaaa
    17881 gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct gatgtatcag
    17941 caagcaccta acctagttga ggaacaatcg agttcgatca gaagtacatt tacagaattg
    18001 gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag cgatactcaa
    18061 aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac agtcaaactt
    18121 gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga tgatcagata
    18181 gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga actacgcagt
    18241 aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca taaaaaacag
    18301 acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga agcgacaaga
    18361 cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct atctgtcact
    18421 cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg actagtgatt
    18481 gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt gcagctttcg
    18541 gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt ttatattaca
    18601 agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga agagggaaca
    18661 gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc tcaagaacaa
    18721 gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc cgaagctgtt
    18781 acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa tgggcaatat
    18841 gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag cacaagagct
    18901 gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag cggtttttac
    18961 aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc ctgatgatat
    19021 cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac aaattgatgt
    19081 ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt agttttaaac
    19141 tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg acagctacat
    19201 gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata cgatcgatat
    19261 gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac gcgatttgca
    19321 tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat gtagaaatcc
    19381 ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa tggcaatgga
    19441 aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat caccgatgga
    19501 agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc atgatgcaga
    19561 tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa cagctatgta
    19621 tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac atggtctgtc
    19681 tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg tcgaaggaag
    19741 acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa gcagcacaag
    19801 agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg atggtgcagg
    19861 aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa aaacagaaat
    19921 cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag cagtgatcct
    19981 tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg cagcagcaag
    20041 aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa tcgttttatg
    20101 cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca tcggcgttac
    20161 cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt ttacccttta
    20221 tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac aaaatcaaat
    20281 cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg cttacttaaa
    20341 gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa gctttgaaga
    20401 agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt ttgaaaacta
    20461 ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga cagcaaccgt
    20521 ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc gacaggcggc
    20581 ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg tgtagaagaa
    20641 gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc tacacctgtt
    20701 acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga agtaggcggt
    20761 gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga gctgagctat
    20821 gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc ttcaacggag
    20881 ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag gaacaggaaa
    20941 acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga aagacaatct
    21001 gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc cagatgtttt
    21061 ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt tgcagacgga
    21121 attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag aagctgaaaa
    21181 aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc ctggagattt
    21241 tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc aatctcgttg
    21301 tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat tgcaagaaga
    21361 gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt taggaaaagc
    21421 agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt atcaatggtt
    21481 cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa aattggtaaa
    21541 atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt tttattatca
    21601 ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac agataaacca
    21661 gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca gttttcaggc
    21721 tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg atgatgatca
    21781 aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg tcatatctat
    21841 tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt cgaatctcag
    21901 caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc caatgacctt
    21961 cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga aaaagacaaa
    22021 aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt gcagttgcgt
    22081 gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa attgattttt
    22141 tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct ggccgctgta
    22201 tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa gataataggc
    22261 ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga tttcgtgcct
    22321 gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa aatttccggt
    22381 ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga gacagcgaaa
    22441 aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg gaaagtcgta
    22501 gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga aaatcctgtc
    22561 gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa tggataagag
    22621 atcattatac gacggattga atcaattaga atcagagttg agaaatacat taacagaact
    22681 tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt tggagatgga
    22741 aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag ctccagctga
    22801 aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt atgaagaagg
    22861 attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc cttgtgcgtt
    22921 ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag gagactggag
    22981 cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta taatcaaaaa
    23041 agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc aattggaaat
    23101 ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct tatcgcaagt
    23161 gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac accccaaaaa
    23221 agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt gttaaaagaa
    23281 ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga tcctggacat
    23341 gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc aggacctaca
    23401 gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt attttatggc
    23461 ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa agaacagaca
    23521 gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac gaatttctta
    23581 gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt ccatgaagaa
    23641 taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc agtaaagggt
    23701 gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga agttgatgaa
    23761 gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga aaaaccaaat
    23821 gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt ctaccgtcag
    23881 taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa tgaggaaatc
    23941 agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa tgaataagag
    24001 gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa aatcattcat
    24061 attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga gtttcgcgga
    24121 catccattag taattgccag acaccccagt gacactggtg ggcgtggagt agtgactaca
    24181 gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca aaaagcgtat
    24241 gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag cgatatttcc
    24301 aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt tagtatcgat
    24361 gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat caaaatcgcc
    24421 cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc gggagtaagt
    24481 tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg aatcacggta
    24541 gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa atttcatggg
    24601 gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg aaaagaccta
    24661 tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta ttctctttat
    24721 cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg aaaatctgtg
    24781 ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct gactcagttg
    24841 cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca tgggaaaaca
    24901 gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt gacattgcca
    24961 gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg ggaggaaatc
    25021 ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct ggacccgatg
    25081 acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa acagctttga
    25141 gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa gtcttgcttt
    25201 ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac aacttgattg
    25261 gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg aaattgtagt
    25321 tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat ttgcgaaagg
    25381 agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc tgtccatgac
    25441 cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg cagacaaaca
    25501 tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta atcgttgcgc
    25561 aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata tcggaaattt
    25621 tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac gtaaagactg
    25681 ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat atagttttgg
    25741 ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa acttctttca
    25801 tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat aaataagaac
    25861 aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga gggcgttcaa
    25921 agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct cttattctga
    25981 ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg aaaaatatga
    26041 ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt tcacaacctc
    26101 atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc atcggacgag
    26161 cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt tttggatcat
    26221 gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca ccttcgaaac
    26281 cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt ttgtcatatg
    26341 caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg tattcacagt
    26401 agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc aatttttcaa
    26461 atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat tctttatctg
    26521 tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg tcagttagac
    26581 tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc catgcatctg
    26641 cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct gggtttgttt
    26701 cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag cctaacgata
    26761 cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa agacgtttcc
    26821 cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct ttacaacgtt
    26881 ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt aactgccaga
    26941 atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat gtaacaacac
    27001 ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac ccttgcaaga
    27061 atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt ttatcgtaat
    27121 tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt ttgatgtcgt
    27181 aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc agtttaggga
    27241 agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa attgcttttt
    27301 gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg aaaggagttt
    27361 gtccatttga agtaaataag gtattgattt catattccaa actttgcata gcatcgaaga
    27421 tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa tcgatccatt
    27481 cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca agcaattcat
    27541 ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca ataatctgtg
    27601 agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca ttgccgattt
    27661 tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc atcggtgtat
    27721 aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca tttgctatat
    27781 gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc agatctcgct
    27841 gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt tggtccttgt
    27901 tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca aaatctcgtt
    27961 tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct agtaatatat
    28021 gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg cttttgattt
    28081 cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt gataatgagc
    28141 cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca tcgaaatcaa
    28201 catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc tgagtaacga
    28261 cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt gttcttgtct
    28321 atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag tattcaatgt
    28381 gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa aaaaggtgaa
    28441 aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa aataattgaa
    28501 taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa ttattcgtag
    28561 atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa gaattggaaa
    28621 agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct cccgtcaaag
    28681 ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt atgggcccaa
    28741 gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg cctacgatgg
    28801 gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag ctcagtgact
    28861 ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat accctaaatg
    28921 caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag gaaatggaaa
    28981 agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa cggtatcccg
    29041 atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt gttgttcagc
    29101 cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg gctacagaat
    29161 tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg ttagtcaccc
    29221 atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat ggtgtgattt
    29281 tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta atagacatgc
    29341 aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt taaaagtttt
    29401 tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc cgtaatgatc
    29461 tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc aagtcaagat
    29521 acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt tttgcttttt
    29581 ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat aggcatctat
    29641 cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga aatcatgata
    29701 attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc caaactcttt
    29761 tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt tatctccatt
    29821 ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt atctgtatat
    29881 tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa agaacaagca
    29941 gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat cgggttacta
    30001 cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc atggatgatc
    30061 agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt tttcgtttgt
    30121 gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat catattgagt
    30181 aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac gcaagttcat
    30241 ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat ttctttaatg
    30301 acaattggcg gcacggctag tatctctact attttatcct accaggagaa agtttcctcc
    30361 ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat tctagcagag
    30421 gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg gagtatctat
    30481 agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga aacacaatat
    30541 caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc acctggtcat
    30601 actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata tggatctagt
    30661 attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag tgtattaggt
    30721 gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt ttttgaaaaa
    30781 gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa taatgaaaaa
    30841 atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt ttattattct
    30901 tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact tccggatgac
    30961 caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc ttccagctat
    31021 tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac actatttgtc
    31081 gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt agcagaagca
    31141 gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca aagaaggatc
    31201 agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat attagggatc
    31261 actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc cgattactgg
    31321 ttggcttatc ttgtgagtgg cttattgatc cttatctatc tt
//
LOCUS       DACEXQ010000008        31162 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.008, whole genome shotgun sequence.
ACCESSION   DACEXQ010000008 DACEXQ010000000
VERSION     DACEXQ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31162
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.008"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..447)
                     /locus_tag="IXH04_000278"
     CDS             complement(<1..447)
                     /locus_tag="IXH04_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HAQ1850913.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFF"
     gene            complement(537..1082)
                     /gene="hpt"
                     /locus_tag="IXH04_000279"
     CDS             complement(537..1082)
                     /gene="hpt"
                     /locus_tag="IXH04_000279"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAQ1850914.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(1082..2527)
                     /gene="tilS"
                     /locus_tag="IXH04_000280"
     CDS             complement(1082..2527)
                     /gene="tilS"
                     /locus_tag="IXH04_000280"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAQ1850915.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(2647..3117)
                     /locus_tag="IXH04_000281"
     CDS             complement(2647..3117)
                     /locus_tag="IXH04_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAQ1850916.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(3163..3588)
                     /locus_tag="IXH04_000282"
     CDS             complement(3163..3588)
                     /locus_tag="IXH04_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAQ1850917.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(3655..3924)
                     /locus_tag="IXH04_000283"
     CDS             complement(3655..3924)
                     /locus_tag="IXH04_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAQ1850918.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(3935..5530)
                     /locus_tag="IXH04_000284"
     CDS             complement(3935..5530)
                     /locus_tag="IXH04_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ1850919.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(5545..9066)
                     /gene="mfd"
                     /locus_tag="IXH04_000285"
     CDS             complement(5545..9066)
                     /gene="mfd"
                     /locus_tag="IXH04_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAQ1850920.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(9083..9643)
                     /locus_tag="IXH04_000286"
     CDS             complement(9083..9643)
                     /locus_tag="IXH04_000286"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAQ1850921.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            9996..10970
                     /locus_tag="IXH04_000287"
     CDS             9996..10970
                     /locus_tag="IXH04_000287"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAQ1850922.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            11140..12540
                     /locus_tag="IXH04_000288"
     CDS             11140..12540
                     /locus_tag="IXH04_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAQ1850923.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(12618..13340)
                     /locus_tag="IXH04_000289"
     CDS             complement(12618..13340)
                     /locus_tag="IXH04_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ1850924.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(13419..13925)
                     /locus_tag="IXH04_000290"
     CDS             complement(13419..13925)
                     /locus_tag="IXH04_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ1850925.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            14048..15847
                     /locus_tag="IXH04_000291"
     CDS             14048..15847
                     /locus_tag="IXH04_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAQ1850926.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(15915..17489)
                     /locus_tag="IXH04_000292"
     CDS             complement(15915..17489)
                     /locus_tag="IXH04_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1850927.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(17744..18253)
                     /gene="mreD"
                     /locus_tag="IXH04_000293"
     CDS             complement(17744..18253)
                     /gene="mreD"
                     /locus_tag="IXH04_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAQ1850928.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(18257..19111)
                     /gene="mreC"
                     /locus_tag="IXH04_000294"
     CDS             complement(18257..19111)
                     /gene="mreC"
                     /locus_tag="IXH04_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAQ1850929.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(19266..19904)
                     /locus_tag="IXH04_000295"
     CDS             complement(19266..19904)
                     /locus_tag="IXH04_000295"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAQ1850930.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(19905..20555)
                     /locus_tag="IXH04_000296"
     CDS             complement(19905..20555)
                     /locus_tag="IXH04_000296"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAQ1850931.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(20568..21467)
                     /gene="rsgA"
                     /locus_tag="IXH04_000297"
     CDS             complement(20568..21467)
                     /gene="rsgA"
                     /locus_tag="IXH04_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAQ1850932.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(21632..23701)
                     /gene="pknB"
                     /locus_tag="IXH04_000298"
     CDS             complement(21632..23701)
                     /gene="pknB"
                     /locus_tag="IXH04_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAQ1850933.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(23698..24438)
                     /locus_tag="IXH04_000299"
     CDS             complement(23698..24438)
                     /locus_tag="IXH04_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAQ1850934.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(24451..25809)
                     /gene="rsmB"
                     /locus_tag="IXH04_000300"
     CDS             complement(24451..25809)
                     /gene="rsmB"
                     /locus_tag="IXH04_000300"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAQ1850935.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(25809..26747)
                     /locus_tag="IXH04_000301"
     CDS             complement(25809..26747)
                     /locus_tag="IXH04_000301"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAQ1850936.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(26740..27231)
                     /gene="def"
                     /locus_tag="IXH04_000302"
     CDS             complement(26740..27231)
                     /gene="def"
                     /locus_tag="IXH04_000302"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ1850937.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(27246..29654)
                     /gene="priA"
                     /locus_tag="IXH04_000303"
     CDS             complement(27246..29654)
                     /gene="priA"
                     /locus_tag="IXH04_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAQ1850938.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(29907..30212)
                     /locus_tag="IXH04_000304"
     CDS             complement(29907..30212)
                     /locus_tag="IXH04_000304"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAQ1850939.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(30217..30831)
                     /gene="gmk"
                     /locus_tag="IXH04_000305"
     CDS             complement(30217..30831)
                     /gene="gmk"
                     /locus_tag="IXH04_000305"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAQ1850940.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
BASE COUNT         8560 a         6724 c         5748 g        10130 t
ORIGIN      
        1 gaaaaagatg atgattacga ttggcaagaa gctaagcaat agggaaaccc atataccgct
       61 gcttgattct tcttttactg ttgtagcaac tttgttttct tttgccagtt ctgttacttg
      121 gctcaatgtt gtatcatttg gcaaaatgat tgttgtgaaa tgggtagtag tcgtatctgt
      181 tgaacctaag atagctaacc caccgctgtt agcgacggtc tgctgttctt tgtattcacc
      241 agtgatctta tatacaccat ttgcaggctg cacttcaaat tctttgatct tgcctgcttg
      301 tagttgttct gtgaattttg agtactcgat atctgacgat cgctggccgt tattaccaaa
      361 aatgaaataa acgaccatta ccatcgctaa gattacgagg acataataca aggcattttt
      421 catcatgccg ttgttctttt tgttcatagt tgccctccct aagcgtcaat ttaatttaac
      481 agttttttat caatcctgac cattctattg taccataatt taattgagat acagcattaa
      541 tttgattgat aaacactagg tttcaataca ccgatgtacg gaaggttacg atatgcttct
      601 gcataatcca agccataacc gaccacaaat tcatttggta cgttgaagcc aacataatct
      661 gcttcgatat ttactactct tccttcaggt ttgtcaagta gtgtaacaat tttgactgat
      721 tttgctttgc ggtatttgaa aagatccact aaataagcaa gtgttcttcc gctgtcaatg
      781 atatcttcaa cgatcaacag atcgcgacct tctacattcg tatctaagtc tttgacgatt
      841 tttacttccc ctgacgagac tgttgcattc ccgtagcttg aaacatccat gaaatccaat
      901 tccaaatacg tatcgataga acgaacgata tctgccatga atggtaccgc tccttttaag
      961 acacctacta ctaggggatt ggtgttttgg taaatttctg ttaactcttt gcctagttct
     1021 ttgcaacgaa cttgtatctc ttcttgtgag atcaaaattc tctcaatatc cttttccaac
     1081 atcaggttct ccttccaatt gcttcttttt gatatttgta aagaagtatg tagtgtattt
     1141 tatcagtttc ttcacgaata ctcaaatatg aaaaggcaaa aggtatcaag ctatatatat
     1201 ttcttttggc atctgtaatg atccaagcat gctgcctttg tgaattaggt actttctgat
     1261 cgataaaata tctagatact ttttttgtga gattctctgt taatttaatg cggtctcctg
     1321 cttttctttt ttcgacatac agttgcacat cagcaggaat ccaaatgtca tgagaaaata
     1381 gagaccatga atctgttgct ttgatgtccc ctgcattttc aggtttgacc agaccaatcc
     1441 attctgtttc attcaaatat attccttgtt ctggaatcag caacaagcgc tggtcttctg
     1501 tctggctagt tgcttgtttt tcttttttag agagtacaac aaaatgatac tctcttttca
     1561 gtatccaatt attgccaata ctgagttgcc attggctttt tttctgttgc cactggtcta
     1621 aaatcgactc catttgttgt tctttcaaaa taattcctgt ttttgagaat gctttgtcga
     1681 aaaaggagta aaaaaaatag tatcgttcag catctgtcat ttcttcaatc atttctgcag
     1741 aaaatcgaaa ttgttcctcc cactgttcga cttccttttc gatcaactgt cccatatatt
     1801 tttgaatgac ttggtctgcc cattggatct gttgcgaaaa ttgctggaaa tgtctcaacg
     1861 cctgtgtatt ttcttgtttc aattgaggaa ctaccaaatg acgaagccga tttctctgga
     1921 tatctaacaa ttggtttgtc tgatcctcaa agtaaacaag ctgcgattcc gctgcataag
     1981 tatataggtc ttctttgcta aaactcagca acggacgaat cagtctgccc gtagcaaatg
     2041 gttgtacttc tttgattcca gaaaaagtat ttaactgtcc cccacgaacg attttcatta
     2101 acaccgtctc gatttgatca tctccgtgat gagcagtcat caacgtgtca tattgtcctt
     2161 cttccatcac gcgagtgaat tcttgataac gaaagttacg agcttctgtt tctatggcgg
     2221 atttggctgg atcttcccaa atacttacat ataaagggag attcttctct tcacaatatg
     2281 agcgaagata ggcagcttct tctgctgatt cttctcttaa ttggtgattg atgtggacga
     2341 tacctaaaga aaaaccgatt tggcgttgca cttgtaaaag taaatggagc aaaaccatcg
     2401 aatcaacacc acctgatacc gctgctaaaa ttctcgatcc ttgttgccag aatccttgtt
     2461 cattcccgtg ccgaataaat gactgctgta atagcggtgc ttttttcccg cctttttcaa
     2521 cgttcatcct ttccacaccc tttcttccta aaaaaaagag agcgggaaaa cgtctaaaac
     2581 gacaattaac ctgccctctg ttgatactat gacccaaata gtcgcactat cattatttgc
     2641 ggctttttag ctgcgacgtc cgccacggcc accgcgcttt ccttctgtgt ttcgcttcaa
     2701 tgaagataag cggtcatcgc tatctttcaa aaatgaactc atcaatgaat cgaagtcttc
     2761 tttttgagct gaaggttgac tgcgagaaaa agattttttc tgtgctggac gatttcggtt
     2821 gtcacgggga tctttttgtt gatatttttt aggacggctt tcttctccag attcagcagg
     2881 ttgttcctgc gctttccgga tagatagtcc gatttttcca tcatcattta ctgaaataac
     2941 tttaactgtt acttcatctc ctacacttaa aacatcatgg atgtctttta catagccatt
     3001 tgacacttcg ctaatatgaa ctagacccgt ttttccagca cctagatcaa taaacgcacc
     3061 aaaatttgtg attcctgata cttttccttg cagttttgct cctacctcga ttgacataaa
     3121 aaaaatgttc ctcctaatta tttccctttt ttatattcct gcttattttg cagttgtaga
     3181 actagttgat tgatctgttt catttgtcga gctgtctgta tttgattcta atataggata
     3241 aactttttca ccgtcttttg agtaaaagaa tttgcttctg gcaagtttag ctacatactc
     3301 atcatctttc agcaatgaaa catcttggtg cagctgatca acgtctgcgt tgacttcttt
     3361 catttcagta tttgcttcga ctctcaattc gttcaattga tgcatccttt gcatgtcatg
     3421 gaaaatctgc acacctatta tcgcgaaaat gaccaaagca ccaacaaaaa ttgcagccag
     3481 tctgcgtctt cgaaaaatca gttgacgttg ttgttgctga aattcagcat actttttctt
     3541 tgcatattcc gtatctagcg cagcgatttt attgcttttt ttcgtcactt ggattcgctc
     3601 caatcttttt gattgtcttt ttcattatac caatatcgtg aaagcttgtc taatctattt
     3661 ctctagtgtt tctgtgcgat tttcagaaat aatttcgtac attttttgtg catcttcttt
     3721 tttggttgat tcatgtaatt ccgtaatttt cacttccaac gttttgttgc caaactggac
     3781 tttcaaaata tcgcctactt tgacatcact ggaagatttc gccaatttac cgttgatttg
     3841 aattcttcct ttatcagcta cttcttttgc tactgatcgt cttttgatga ttcgggaaat
     3901 ttttaaaaat ttatctaatc gcatatctta ttcctcactt tttaattttt ttatttaatc
     3961 gcaggatttt cttaccaaaa ggcaacatta gccattctcg tactgtaaac agacgaagac
     4021 gaatcactgc ataaatgaac acagaagccc cgatcccaac tcctatcacg ctgacaaaaa
     4081 aagcagtgga acgatgagtt acaggaccaa atactatatt caatacacca taatacagca
     4141 gcagtactac tgtcatacaa agaaggctga acatcaactt ccgtccaaaa tgccgttctt
     4201 tccaaaaagc atttagctct tttgtttcaa ttcgaacaaa ataccaaagc gtcacaccta
     4261 atcctaaaag ggtagacagg cttgcacctg ctgttcctaa taaaccggtc agaaaagaag
     4321 tagtcaatcc ttttaccaat agtccccatc ctgcgccttt taaagaaggg cgaaacgagt
     4381 tcttgctttg agcaatactt tgataagctt ggatgactgc agtgaaagca atggaaaaaa
     4441 caaataatac aagcgctgca tttcccgcat aatctttaaa caacgcataa ttgatataag
     4501 ggagcagcaa ggctaatccc aaagacgcag ctaatgctaa agcggtcgtc aatctaagat
     4561 agattttggc tgtctgtaaa aactgacgat agatccggtt ggtcaaatga cgagtcaacg
     4621 caggcaggaa tgtcgcactt aatgcagtgg caatcactaa acctaattgt actaaaggct
     4681 gacctcgatc atagacccct tttgctatct tcgcaccgta ttctgacagt ccgtaaactt
     4741 ccaaagcatt ttttacaaag aaagagtcga tcagctggaa aaagataagc aatccactat
     4801 aaatcgaaac cagtccgcct tctaccagaa aacgccggat caagggcttt gacggctgtt
     4861 tgaacaatgg aaaccgtcgg aagcttaacg ctcctccatg aatcttttgt tcatagtagt
     4921 gaaggatccc atatgcgcat aaaccgccaa aaacagctcc actcattgct gccgtccctg
     4981 tttggtacac cgtccatcca agctttctaa aagcaaacgc actacagaca atcacaaaaa
     5041 cacgaacgaa ctgctcaact acttgagaaa cagcagttgg ttccatcaag aatcttcctt
     5101 ggaaattccc tcgataaaaa gatagaccag gcatcagcaa aaaggtaaag gaaactactt
     5161 ggatcagcgg tttcaattgt atgtctccca tcatccaagc aatccagctg ctaaatgcaa
     5221 agacaaacaa ccacaaaaag acacctgtcc aaaatactaa gggataaaga ttttgcaaag
     5281 cttgctttcg ctcgaaagga ttctttcttt ctgcaacgta ctttgatatg aactgcggaa
     5341 gaccggataa agctaaagtc attgctatcc cataaattgg ataaacttgc tgataaacat
     5401 aaaaaccttc gtcgccgact aagttttgga ggggaattcg gtaaagggca cttaatactt
     5461 ttgctatgaa agaagcaatc gtcaatacaa atgctccttg catgacgcgc cgcatttctt
     5521 tatgatccaa atcattgctc cttcctacgc tgacagatat ttttgctcac gcaacgcttt
     5581 gacaaacttt tggatttcct gaagccatac tgcttcttcc atttggttgg ggatcgtcaa
     5641 gcgtatcacc atctgttctt tttcaacgcc tagactggct tttagttttg tcgctgtcag
     5701 tgcttcgaat aactgttcca cactatagcg ttttgttcca actttactta gagtaaagac
     5761 tatatgcgcc tcctgtttcc ttatcgtttc taggagcgct cgatcgccat ccattttgat
     5821 ctgcccaatt gtcaagagat gtgctacttc ttctggatat tctccaaaac ggtcgagcag
     5881 atcatcttct agttcatcta gcatttcttg cgaatcaagt tcacgaattc gtttatagat
     5941 ctcgattttc tgccgttgat ctgccacgta tgtttcaggt agatacgcat cgacaccgag
     6001 gtcgatctca acagtggtct tctccacttg actattcttt ccttgtttac gagaaacagc
     6061 ttcgcttagc atttgagaat acatatcgaa accgactgca tcaataaatc catgctgctg
     6121 agcacctaag agattacctg caccgcgaat ggaaagatcc cgcatcgcaa tcttgaatcc
     6181 agatcccagt tctgtaaaat ctttgattgc ctgaagacgt ttttcactga cttcgttaag
     6241 gatcttctgc tgctcataca taaagtaagc atatgcgaca cggctgcttc ttcctacccg
     6301 ccctcgcaat tggtataacg tggataatcc catataatca gcgttctcaa cgaacaacgt
     6361 atttgcgttc gggatatcta ctcctgtttc aatgatggtt gtagtgacaa gaacatcata
     6421 ttgaccttca ataaattcaa aaagcgtatt ttctaattgc gcctctgtca tttgcccatg
     6481 agcataaccg atccgtgctt ccggaacgag ttcttggatt tcttctactt tttgttcgat
     6541 cgtttctacc cgattatata aatagaatac ttgtccacct cgtcccattt ctcgatggat
     6601 tgcttcgcga atagccccag gatttttttc cataacatac gtttggatcg gataacgatt
     6661 cgcaggcggt gtttcgatta ctgaaagatc ccgcacgcct aacatcgaca tatgcaaagt
     6721 tctcggaatc ggtgttgcag tcagagtcaa aacatccact tgtgcacgta actgtttcaa
     6781 tcgttcctta tgttttacac caaaacgttg ttcttcgtca atgatcagta gacccaaatc
     6841 gctaaattca atatcttttg acaaaattcg gtgggttcct acaacaatat caacttgacc
     6901 tgtacgcagt tgttcgatgg tttctttttg ttgctttctt gttctgaaac gacttaatag
     6961 cccgatattt acggggaaac cttcaaatct ttccagcatc gtttcatagt gctgctgtgc
     7021 caagatcgtt gttggaacta aaaaggctac ttgtttgctt tcctttatcg ctttaaaggc
     7081 cgctcgaagc gctacttctg ttttcccata accaacatca cctacgagta gtcgatccat
     7141 tggtttttct ttttccatat cccgtttgat ctccgctgca cttctcaact ggtcatcagt
     7201 ttcagagtag gggaaggcat tttcgaattc tttttggtag gcgtcatcag gtccaaaagc
     7261 atatcctttt tctgactctc ttgcggcata taatttgatt agatcgtcag cgatatcctc
     7321 gatttttgaa gagacttttc gcttggtttt cgtccattca cttccgccta gcttattgat
     7381 gcgaggcgct ttggcttcag aggcgacata tttttggatc agattcagtt gagtaacagg
     7441 aataaacagt ttgtcgtcgt tttgatacaa aatagtcata tagtcttggt gaacgccatc
     7501 tacttctagc gtttccatcc caatgtattt cccgatacca tgattggcat ggacaacgta
     7561 atctcctgct tttaactcat tatagctttt caatcgctct gcgtttgtta ctgtttgacg
     7621 tctcgtttgt tttttcgtcg ttttttggaa aatttctttt tctgtgatag ctactatttt
     7681 ttcttgagga agttcaaaac cagcctgcaa agctccttca actaattgta cctgtcctct
     7741 gatcaatgca tttttttctg tttctactac taatatgtct tcatctcgaa gcatttgttc
     7801 tgctttgcgt attctgtcct ttgtcggaat aaagatcaca acagtctgtt tttgcttgcg
     7861 ccaacggtcc atttctgttt tcagcaatgg catttgtccg aagaactgtt gcatcgtccg
     7921 atattgaaaa ttatggattg cttggaaacg aagatttccc atcccttttt ggaagagtga
     7981 gaaaaaggtt gtggtgaact tttccttctg cacgattttt tgtatatctg ctgaaaatgt
     8041 ctgctcagaa aacacccgca attcgctgat tttttgagtc tgccattctg ccgcttccct
     8101 ttcgatttcc cgatttgttt ccatgattcg ctgataatca tcaacgacta gtaaactatt
     8161 tgaagggaaa taatccaaga tcgtcacaga ttcttggtaa agaagatctg agtagaattt
     8221 tgcttcttct gtcgggacac ctgcttgcca gcttgaagtc agctgaccga agtaatcttg
     8281 caaaaactcg cggtctgctt cttctgtggt cgcagcaagc cgtttttcta gcattttttg
     8341 catttgctct attccatgtt ctagatcctc tggtccgtag acttgttcac tcgttgggga
     8401 aacccagatt tcttccgttt tttcgattga gcgttgtgtt tctgcttcaa aataccgcat
     8461 agaatctatc tctacatcaa atagttctac acgaacagga taggttgtcg ttaacgggta
     8521 aatatcgata atgcttcctc gaatactgaa ttctcccggt tttgcgacca tctcttctcg
     8581 tacatagccc atcaaagtca attggcgagg aagattttcc acgtctatct catcaccaat
     8641 atgccaattc aattgcgcct gtagccaagt tttcttattc ggcagtcgtt tgtgcaaaga
     8701 tgcgactggc aacaaataga tacctgcttt tttttcttga agtgcgttaa gcgttgcaac
     8761 tctctcgcct cgcgcttctg gagaagaaaa ggccatttca gcagaaagca cttcatccac
     8821 tggaaataaa tggatctgat cttgcggaat cacatgttgc agatcttctg ctaactgatt
     8881 cgcataatac aaatttggca tcagaataat catgttttta tttgttttct taaacgctcg
     8941 agcaaataga agtgttttcg ctgagcccgc taatcctgtg attagttgtc taggcatttt
     9001 ttcttctaaa ttgctgaccc attcttttac ttgcggatct tttccgacta attgaataat
     9061 atccattcct tttcctcctt cctcaattga atcgattcat cgcatccacg aacgaatgcc
     9121 cttctatgag atattcgact gctttaacag attgacacat gctttcttct atcaaaggct
     9181 gattttcttt gctgaaagga ctaagcacat gctggactac tgtttttttc ccttctggcc
     9241 tgccgattcc caccttgatt cgatcaaaga cattcgtatt caaatgggaa ataatacttt
     9301 tgataccgtt atgcccgccg gcacttccct tttgacggag ccggattttt cccactgcca
     9361 gatccaaatc atcgtagatt acgactaatt cctctggata aatcccaaaa taagtcatta
     9421 gtgggccgac tgcacgacca gattcattca tgaatgtttg tggtttgact aaaaggatct
     9481 tttctccatt atgaaaaaat tcggcaactt ctgcttcgaa cggatttttt ttgaaagtag
     9541 cttgatgctc gcgtgcaatt ttatccacga ccataaagcc gatattgtgt tttgtatatt
     9601 ggtatttggt acctgggttt cctaacccga cgatcatttt catttttgtt ttccacctaa
     9661 ttctaaaaag cagcacaaaa ggctggactg ctaattagcg gtccagtcca tcgagctttt
     9721 ttctttttta tacttgtcaa tgcatagtat aacacaaaac aaggcgtttc ggctaaaaga
     9781 gaatcgttaa taaatgataa agaaattaaa aatttcttca aagtttaatg aaataattga
     9841 tatataaaaa tagtgaaata acgcacatgt aagcttttac atataataca accttgttat
     9901 ctgttatatt ttatagccaa atagcgtgac atggggcttg agaaatgtta gcataagcat
     9961 gggaaaaaac aaatcaatgg aaggattgat actaaatgac tgcagctgca gaaaaaaaag
    10021 atcatcaaaa agttattctt gttggagacg gcgccgtagg ttctagctat gcctttgctc
    10081 tagttactca aaatatcgct caagaagtag gtattgtgga tatcaatact gctaaaactg
    10141 aaggagacgc aattgacttg tctcacgctt tggcatttac ttcacctaaa aaaatctatt
    10201 ctgctactta cgcagacgca catgatgctg acttagttgt tattacagct ggcgcaccac
    10261 aaaaaccagg tgaaactcgt ttagacttag taaacaaaaa cctaaaaatc aaccgcgatg
    10321 ttgtcacaca aatcgttgaa tcaggattta acggtatctt tttagttgcc gctaacccag
    10381 tagatatttt gacttactca acttggaaat tctcaggttt ccctaaagaa cgcgtaatcg
    10441 gttcaggtac ctctcttgac tcagctcgtt tccgtcaagc acttgcggaa ttagttgacg
    10501 ttgatgcacg taacgtccat gcttacatct taggtgaaca cggagattca gaattcccag
    10561 tttggtcaca tgcaaatgtt gccggcttgc aaatctatga atgggtaaaa aacaacccag
    10621 aaatcgatga agaagctatg gttaacctat tctttggcgt acgtgacgct gcttacacaa
    10681 ttatcgagaa aaaaggcgct actttctatg gtatcgcagt agcattagca cgtatcactc
    10741 gtgccatctt agatgatgaa aatgctgttc ttcctttatc agtattcatg aatggcgaat
    10801 atggattgaa cgacatctac attggtgcac ctgctgttat caaccgtcaa ggtatccaaa
    10861 aagtaatcga aattccttta agtgacagcg agcaagaccg tatggctgct tctgctaagc
    10921 aattaaaaga aattctagat gaagcttttg ctaaactaga cgctgaataa tctttgaaca
    10981 tgacggaaaa catctattcg gtgttttccg tttttttatt ttatcaccct tatggtttct
    11041 aaattttttc ataaagtaga gataaccttc gtcttttcct ctaaattatg ctatcatgag
    11101 accgttccac cctaaacttt tacttaagga gttgtcgata tgacaagatc ttcgcatcga
    11161 aaaaaatcga aacatgttgc tttgctttca gttcttggtg ttctcttagt gctgatcagc
    11221 ttttatacct atcgcagtac atactacaca aagcattttt tgccgaatac agaaataaat
    11281 ggaataaatg tcagcaatct aacagtcgaa aaagcaaatg aaaaattaaa agaagcttat
    11341 tctgataaaa aactggctat taaagaagat ggaaaagtct ggcaggaagt accaaaatcc
    11401 gagctcggct acaaagatga tttcacttcg gagctttctc cgatcctaaa tgaacaaaac
    11461 ggatggactt ggggaatgac ttatgtctca gcggcagaaa aacaagaaat agatcctgtc
    11521 agtcaaaacc accaaaaact agacactacc gttcagacat tgacaactaa gctgactgat
    11581 ttgaacaaag accggacacc tactactgat gcaacgatcg aaaaagcagg tgactctttc
    11641 gttatcaagc ctgaagtcaa tggaaatacg atcgacgtcg atgctgcggt caaacaatta
    11701 aaaagtgcgg taaactctgg taaagataca attgaattga ctgaattcaa agaaaaacca
    11761 aaagtaacat ctgaagacag ctcactgaaa gaacaactcg catccatgaa tgctatcgca
    11821 aatgtaaaag ctacttacag tataaacggt gagactttcc agatcccatc aagtgatatt
    11881 atgagttggc tgacctacaa tgatggaaaa gtcgatttag atacagaaca agtacgtcaa
    11941 tatgtaacag atttaggcac aaagtataac acaagtacga atgacacaaa atttaaaagt
    12001 acaaaaagag gagaagttac ggtcccagtt ggcacttaca gttggacgat ccaaacagac
    12061 agtgagacag aagcattgaa aaaagcgatt ctggcaggcc aagattttac tcgttcaccc
    12121 attgtgcaag gtggcacaac cgctgatcat ccattgatcg aagatacgta tatcgaagtt
    12181 gatctggaga accagcatat gtggtattac aaagacggaa aagtggccct tgaaacagat
    12241 atcgtttccg gaaagccaac tacacctaca cctgctggag tcttctatgt ttggaataaa
    12301 gaagaagatg caactttaaa aggaacaaat gacgatggaa caccttacga gagtccggta
    12361 aactactgga tgccgattga ctggacaggt gttggtatcc acgattcaga ctggcaacct
    12421 gaatacggtg gagacttatg gaaaacccgc ggttcacacg gctgcatcaa caccccacca
    12481 agtgtaatga aggaattatt cgggatggtc gaaaaaggaa cgcctgtatt ggtcttctaa
    12541 caaaaataat atataaaaag aaagcacccg tacgattggt acaggtgctt tctttttatt
    12601 atctaacaat gtgctactta catttcagca gcagtaaaga cttctgaaac atcatcatcg
    12661 tcttctagct tatctactag acgttccaat tgctcttttt gttcctcatt taaatcaatc
    12721 gtcgtttgtg gaatcatcgt caattctgct tgcgccaaag tgaactcttt ttccaactca
    12781 tctcgtacag cagcaaaatc ttctggtgca gtataaatct cgaatacttc aggagaagtt
    12841 tgcagatctt ctgcccccgc ctctaataca tcctcaaaca tcgtctcttc atcaacagtc
    12901 aacccatcgc gttcaatagc gatatatcct ttgcggtcaa acaagtaact gactgaaccc
    12961 gtttctccca gtgatccacc gtttcgagta aaagctactc ggacatttgt cgctgtccga
    13021 ttacggttat ctgtcaatgc atggactaag attcctactc cacccggacc atatccttca
    13081 tacgtgattt catcgtaatt cgtttcatct acggcactcg tagccttttt aatcgcccgt
    13141 tccacattgt cgttaggcat atttgcagct tttgctttgt ccacaacaag ccgcaaagct
    13201 ggattcatag atggatcagg accgcctgct ttagctgcca tataaatttc acgagaaagt
    13261 ttctgaaaga ctttccctcg ttttgcatct tgggcatttt ttcttccttg gatattatgc
    13321 catttactat gtcctgacat gatttttcct cccttgttct tttgagaata cacttctatt
    13381 ctagtactat ttcacaaaaa aacaaagcca cagccaatct ataaacgctt cttgaatgcc
    13441 gcaaaaagca cacataatat cacagcagtc agagcaccga tcgaatccaa catgacatct
    13501 tggaacatag gtgttcgtcc accggtcatc atctgatgga attcgtccaa acctgcgtac
    13561 cctgttgctg aaagccaagc aaggatcgct gtcagccacc aaatttttag cttggggaaa
    13621 acacctaaaa acaagctgcc tcccaaaata aaataggtac cgaaatgtgc gcccttcctt
    13681 aaaaagaact ctaaaaattt ggcgtagccc agatggtcga tactcaccat acttccacca
    13741 taattaaaac gtatacccgc taatgtgtct ttaaatggct gattaggcag ccagctttct
    13801 aatcgcgaaa cttgtgattg ctgtccgtaa gtttgagaag aactgtaaaa taagattata
    13861 acgactatca gtgcgattac taaataaata tttccgtttt tcatttgttt ttttgaagaa
    13921 ttcataagtc acctctgtat tttatcttag actttttttg aagaaacaac aatcattttc
    13981 atagagactt cttccataaa aaagtgctat acttaatttt gtattacaat atgaagggag
    14041 ttttttcatg agttattcat taaattggaa tctagattct attttttccg gaggcagtca
    14101 ttctgatgct ttgaaccaac ggatgaagca attggaagac caaatgaacg agtattatca
    14161 ccgcgtaaca aaatggtcac ctagttctga caatgcagag cagttaaacg ctattcttca
    14221 attgcaagaa acgattacga atggttttac ccaatgcagc agctatatta ctgcattgct
    14281 ttctgccaat gtcaatgatt cagatgcaaa ggtcttatca ggcaagcttt acgcaatgct
    14341 gcctcgtctg caatcagccg aaactgtatt gtcaaagaaa tttgcagaaa tatctgacaa
    14401 tgattggaat cagatgcttt ctcaatcttc gtttgaaaca attgcttttc gtttaaacga
    14461 gattcgtcgt gatggctcac aattattatc agaagctgaa gaaaacatta taaatacgct
    14521 ttctttagat ggtctaaatg cttggagcag tcattatgat acgattgtag ccagcatttc
    14581 gattcccttc gaacaagatg gacaagtagt cgaattatct gctggacaag ccttcaataa
    14641 gatgatgggc gatccagatc caaaagtcag agaaacactt ttcgcagcat gggaagaagc
    14701 ctggaaagaa aaggcacccc tcttcacgga tactttgaat catttagatg gttttagact
    14761 ttctgactat gaacttcatg gtgtcacaga ttttctacaa aaaccattag agtacaatcg
    14821 gttgaagaaa gaaacattgt ctgtcatgtg ggatacaatc cagaaaaaca aacaacccct
    14881 tgttaattac ttgactagaa aagctaatct tttcggtaaa gaaaaaatgg aatggcaaga
    14941 tcaagatgca cctattattt taggcgattt gaaagaaaaa acctttagtt ttgacgaagc
    15001 agcagcattt attatcgaaa actttaacaa attcagtcca aaaatggcta aatttgccca
    15061 atctgctttt gaaaagagct ggatcgaagc agaagatcgt cctgggaaac gtcctggtgg
    15121 ttattgtact gagttaccag aaacaaaaga atcaaggatc tttatgacct acagtaattc
    15181 tgttaatgag gttgcgacac ttgctcatga actaggacat gctttccata gcagcacgat
    15241 gtgggattta ccttctttaa atcgcgaata tgccatgaat gtcgcagaaa cagcaagtac
    15301 attcgcagaa ctgatcgtag ccgatgctac attaaaagtc gcaaaaacaa aagaagaaaa
    15361 aatcaatttg ctagatacaa aactgcaaaa tgctttagct atgttcatga atatccattc
    15421 ccgattcatt tttgaaaatc gattctatga agcacggcaa gaaggattag tctcagaaga
    15481 taagatcaca gagatgatgg ttgaggcgca aaaagaaggc tatcaaggag ctttagctac
    15541 ttaccatcca tatttctggg ctgcaaaatt gcatttcttt attgatgacg taccgtttta
    15601 taacttccca tacacatttg gctacctctt tagtttgggc atctatgctt acgccaacca
    15661 aaagggagca aattttgaag aagaatatat ccatttacta cgcgatactg cctcaatgac
    15721 gacggaagat cttgcgaaga aacatttgaa tgtcgatctt acccaaccag atttttggca
    15781 agcaggtatc gacatggtga ctgaagatat ccaaacgttc atggaattga cagaagaatt
    15841 tatctgattt ctattttatc acaataaaaa aagagttagt ccagaatggg ctaactcttt
    15901 ttgattaatc attcttacat gctgacagca aagtcagggg caaaccattg aactgaacca
    15961 acttttacgc tttcgcctgg ttgaggagca tggatatatt gtcctccgcc taaagcaatc
    16021 gctacgtggt aagttccgcc ttgtgacccc cagaataaca agtctccagc ttttgcttga
    16081 gaaactgaaa tttttgttcc agctgattct tgaggaactg tccaaccacc gatgtcacga
    16141 ccagttactt gcatgtaaac atagcgtgtg aaccctgagc agtcaaagcc acttggatct
    16201 ttaccgcccc aaacatatgg agtaccaata tatttgtaag cttctgctac gattgctgca
    16261 ccattcacac ttccagaagg agctggagca ggtgttacag gttttggttg ttcaggtgtt
    16321 ggtgttggcg ttggagccgg agtactactt cctgtgccgt ttcctgtatc aacactttga
    16381 tctgttgatg gtgttgtagg cgcaggtgtt gttgattctt ctgttgttga agattcaggc
    16441 actgttgttg attcttctgt tgctgaagat tcaggcactg ttgttgattc ttctgttgct
    16501 gaagattcag gcgctgttgt tgattcttct gttgctgaag attcaggcgc tgttgattct
    16561 tctgttgaag attcaggtgt tgttgattct tctgttgctg aagattgtgt agctgatgat
    16621 gattcttcag tcattgaaga ttgtgtagct gatgattctt ctgttgctga gctttgtgca
    16681 gtagatgaag cttgagtagc ttgtgcttca gcctctgctt gttcgcgtgc ttctttttca
    16741 gctttttctt gtgcagcttg ttgacgagct tgttctgcta gacgttgttg ttcacgaata
    16801 cgagcttgct ctgcttcagc ttctgctttt tgacggttca agtcagcttt cttgtcttca
    16861 gcagttgctt gttcagcagc taatgaagtt ttcagtacgt tcaaatcagc ttgtttagat
    16921 aaaagatcgc ctttttgaga ttccaaagca gcttggtttt cagctaattc ttttagttta
    16981 gcatcgtttt cagctttttt gtcttctaca gcttttttat cttgtttttg ttgttccatc
    17041 aagtcgttgt ttgcttttac catcgttgtc attgcttgaa cgcgtccaat agcatctgct
    17101 aatgaatcag cattcaatac agcatcgatg tagtttgagc ttgtgttgct tacttgtgct
    17161 tcgcgtgctt gtttttggat tgtatcttca cgtttttcaa tacgttcttg taaatctgca
    17221 atgtctttta ctaattgtgc agattcttga cgcaatgtat cttgttttgc taataggtct
    17281 tgtgcttgtg tgttgatttc agaaacttgg ctttctaagg catcgatttg tgattgtgca
    17341 tctgcttgtt gatttttcaa gtctgcaatc ttttgatctt gctgttgtat ctgagaatca
    17401 aaatcgtccg ccgcggcagc gatcggtgat gctacggcag tcaaggtcat tgaacatacc
    17461 attactgctg atattaaact ctttttcact cttcattcct ccgactggct taatttaata
    17521 aatatctaaa tatatttaag tctttcttaa cgatataaaa attgtaccat agcagtgtgt
    17581 caacgatata atagttatgt tacaaaaatg tttcaaaaca acttttaaaa atgacaaaac
    17641 tgttcgtttc agaaaatgat catgaaaaag ccgcgttaag tctagcattt cttaagataa
    17701 caacgattct ttttcgcttt cgacctaaaa ataaaaaaag aaattattca tcactgaata
    17761 atttcttaaa gggaaaaatg aataatacaa atataatcat gttcaacagc agtgtaggtc
    17821 ctaaaaacct tgtaataaaa tacgtgttat ttactactgc taatttaaaa atcaactgga
    17881 ccaccatcgt aaacaattca taacccgtga caaagatgat catactgaaa aattcagtaa
    17941 agatgttgac atggataacg tctttcatta catacatcaa ccatacaatt aaagggagag
    18001 ctactgcata gataccgata acgccaatat agtaagcgtc atagatagcc ccgagaacaa
    18061 tcgtggtaat cagtaaataa cgtttttcaa acgccatact gcaacataat aatgccaata
    18121 tcagaaagtg ggcatttgac atataggtgc ctttcgacca ctcccctagc attcttgtta
    18181 aatgaccatc tatcaacatc aaaaagaaca aaacgattgg cagatagtac ttcattgttt
    18241 ctttcttcaa catggactaa tcctccgcta gccgttggac aatcgtcaca actgacacgt
    18301 catacatttc tgcataaggt ttcacataaa cttcccgatc cagcccataa ctatctggtt
    18361 tcgttttaat aaccgtaccg atcggcaagt tagctggaga attccctcca agaccggatg
    18421 tctggacaac atccccttct ttgatatccg tatcaccagt taattgggtc accactaagg
    18481 catggagctt ttcatcatag tttttaagca aaccaaacgc ttcgccatta gccgaagata
    18541 cccgtactgg aaaatgattg gagctttcat tagaggatga cagtaattcg attttagacg
    18601 aagccgtatt gacctcgatc actcggccaa tcaatccttt ttgcgacatg actgccatat
    18661 ttgcttcgat accgtcttta gaacccttgt ctacgatcag catgtcctgc caactgtcag
    18721 gcgaacgagt aatgacattt gccgttactt tttcgtaact agtcagcgtc tgattcaact
    18781 caagttcttt tttcaacgct tcgatttctc tctcgtaatt cttgttttgc tgtgaaacgt
    18841 catcatagct atcgattttt tcttttagcc gctcattttc ggcataagtt gaaaaaagat
    18901 tatggacaga atctactcca tttcgtaccc accgtactgg ggcagagatt gttttatcaa
    18961 ccattgcaac actgtcatta acgatggatt ggaagaaatt gggctttcct tcattcgctc
    19021 gctgcgcagc ggtcacactt agtatcgtca ccacgatgat aacaataacc aaggtaataa
    19081 taatattttt attaggattg aacttcttca caaagacacc tcgaattaat tagactcttg
    19141 tgttcaattt tatcactaaa tgccgtgaaa caaaaggtaa gttgctcctc ttaagctttt
    19201 ttttagcaaa taatagattc tgtcattttt agcctgaaag acaaattttt ttatttgttt
    19261 atggatcaat ctttgctttg aataactgca atcataccat ctgaaaaaga aaattctgcg
    19321 gtatctgaaa gaaattcgtt acttgcataa gatgtcggat attctacttc tttattttcc
    19381 aatacatact tgctgccttt taatgtaaga ttcgagacgg gagtcaagca gcaataagct
    19441 aaatacttca tatctggtat ctgtcttact atatgctctc caggcaaata ataagacaaa
    19501 taattttgac aatctctgat aataagctga tgggcaaagg gtttgaagcg aggttccata
    19561 cctaaccaga gattggctaa aagatgatcc aatcttccgc ccgtagctcc aatcaaagtg
    19621 acttccgctt gcgggtaacg ttcaaatgtt tttaagatac ctaactgtgt atctgtatcg
    19681 tctttttctg caggggaacg ggtatactct ttggcttttg caaatacttc cttctgctct
    19741 tcgttcgaca atgaatcaaa atcgcccact gccagatcta ctgggtagcc tgcttgaagc
    19801 aggtacagcg caccacggtc tatcccgaca taggcatcgt atttggacag cagagactgc
    19861 ggccagtttt gcggattacc tccagcagct aataaaattt tcatttagtc taatgcctct
    19921 tttaatcgtt cgatttgtcc gactggattt tcggctccat agatatatga tcctgcaacg
    19981 aagacatccg cccctgcatc tttacagatt tttgctgttt ctggaacgat tccgccatct
    20041 acttcgattg cataagtcca attgttctct ctacgtagat cagcgagttc cgtaattttt
    20101 tctactgttt cttcaataaa gctttgtccg ccgaacccag gattgacagt catgactagt
    20161 acttgatcag ccaaaggcaa aacatgtttg atcatggaga ctggtgttcc tggattgata
    20221 actactccag ctttaactcc taagcttttt gtcatttgaa gtgcccgatg aatatgagga
    20281 gttgcttcga cgtggacagt aatgatatct gctccagctt ttgcaaatgc tggaatgtag
    20341 ttttctggat caacgatcat cagatggacg tctaacggta atttagtaac aggacggatt
    20401 gcctgaacaa tatttggccc taatgtgata tttgggacaa attggccatc cattacatct
    20461 acgtggatgt aatctgcgcc tcctttttcc actaactcaa tgtctctttg taaattggca
    20521 aaatctgcac tcaaaatgga tggtgctatc ttcattcttg tttcctccta ttttcgttta
    20581 ccttcttttt ttgcatacat tggttttctt ttttcgattt cttgtaagaa ttgcaaataa
    20641 ttgtcgtaac gtgtctgtgc gataccacct gtttctactc tatgttttac ttcgcagcca
    20701 ggttctttag catgcatgca ctctctaaat ttgcaatgga cagaagcttc tacaaattca
    20761 ggaaattgtc tcggcagttc tcctgcttca attgtcaaaa agtcaatcga gctaaatcct
    20821 ggtgtatctg ctactaaccc gccatagata ggaatcagtt ccacatgacg tgttgtatgc
    20881 cgtcctcttc caagtgcatc agaaatctct cctgttgcta aattcagttc cggtgatatt
    20941 tgattcagta gcgtcgattt tcctgctcca gactgaccca taaagaccga tagccgttct
    21001 gggaagtaac ggatcagttc tgtcgtatca cccgtatgat tgggtacaat caccggatag
    21061 cctattttag tataggtttg cttgatctct tcgatttttt ctgggtcgtc tgacaagtcg
    21121 gtctttgtca aaaaaataat cggttcgatc gactcgtatt ccaaggtaac tagaaagcgg
    21181 tcaagtagat tataagaaaa tgcaggctct acgatacttg tgacaatgat tccaagatca
    21241 acatttgtga ctggtgggcg aactaattga ttttttctcg gcaagatttc cagaagatag
    21301 ccgtctgttt gattttcact ctcaaagatc acctgatctc cgacaagcgg cgtgattttc
    21361 cgattccgga aatttcctct tcctcttgtt tgataggtct ctccgtttgc gtatacatag
    21421 taaaaaccgc tgattgcttt tctgatttgt cctttgataa gtgccacctc cgtttttgtt
    21481 tattttacca tacgaagagg aaagtaggtg ttattccatg caaacttctc aaagtatatg
    21541 gcttgaaaat tgatcaaaag agatagaaac acaaaaaaga gaatgcaaca aatcaatgtc
    21601 gcattccctt ctgatatatt ttattcagca cttattttga actttctgag gtgctagctg
    21661 atgaacttgt ggagctggaa gattcttctt ttgaagagtt gcttgtgcta ctggagctac
    21721 ttgagctgct agaactgtct gaagagtctt tttcagaact agaagacgct ggatctggtc
    21781 ctttagagta aattacgttc acaaccattc ctttggtaat tgtcgttcct gcccctggta
    21841 ctgtacgaat cactttatct ttatccaccg tgtttgaata ttcggaagtc tcatgcccga
    21901 tatattctgc accgacacta gctaaataac tttgtgcttc gctttttgta tagccggaga
    21961 tatcattcaa tttaggctct gttccttggc ttacgacaaa attgatgtaa gtttcttctg
    22021 ctacaacttc acccgaggct ggatcctgac tgatgatcga acctgatggt acagtgtcgc
    22081 tatagtcata agtagcacta atattattga tattgaatcc ttgtgtaaca agccaattac
    22141 gaacagtttc gtagttttct cctacatgac tactcaaagt cactgtttct ggtcctttgc
    22201 tgactgtgaa agtaatcgtg tctgttttag gatcgacttc tgtatcttct gatggagatt
    22261 gcgaaatgat cattccgctt tctacctctg aactgaattc ctcttctttt tgaatcaagt
    22321 cagcagaaaa ccctaattct ttcaaacttg cttcggcatc ttcataactt ttaccactgt
    22381 aatcatctaa tttgattttt ttcgtgccgc tactaatata cagagtgact tcgcgttttt
    22441 gcttgactgt cgttcccgct gccgggtctg tcttcactac attaccttct tcgattttgt
    22501 catctgcaat ttcttctgtt tttgttgcta cttctaattt cgcttttgcg agtgtttctc
    22561 tagcagaagc ttcagaaaga tcagaaacat caggaacaac aacttctcct cgcccgccag
    22621 aagcaaagaa tacagctgtt ccgattccta aaacagcaag aatagctaat aggatcagcc
    22681 accatttttt ctgtcctttc tttttaggat caggcagaac ttcttcctcc tctggcaagg
    22741 tttcgttttc tggcttgtct tttggcagag gcatagattt gaatgaatca ggcattggtg
    22801 catcttccgt aaggggtgtg ataacttttg tttcattatc catcacatga ggctgccatt
    22861 tttcttcgtt tgcacgagct ggtgataata ctgtagaaag atcatcagac atttcctcgg
    22921 ctgacttata acggtctgca ggttctttag ccgttgcccg taaaatcacg ttttccagtg
    22981 cttgtggcac atttggatca agtgctttta tagatggaag atcatcttga aaatgtttta
    23041 aagcaattgt tacagcagat tcaccatcaa acggtacgct acccgtcaac atctcataaa
    23101 ggatgatccc taaagcataa atatctgact gcttcgttgc catacttcct ctggcctgct
    23161 caggggacag ataatgaaca gatcccagca tcgtattcgt ctgcgtgatc gatgtttcag
    23221 ataaagcgat agcaatcccg aaatccgtga tttttacaac accttcatta tcgatcagta
    23281 cgttttgggg tttcaaatct cggtggatga tttgatggtt atgagctaaa gaaatagcag
    23341 aaaggatctg ctgcataata tttactgctg tttcataagg aacaggataa tgtgtttgga
    23401 tatatcgttt caagtccatt cctttgacgt attccatcac cagatactgc atgttgtctt
    23461 cttcaccaac atcgtaaaca ctaacaatgt ttggatgaac gagttctgtt gctgctaaag
    23521 cttctctctg aaatcgtcga atcgcagtct ggtcattttg aaagtcaaat cgtaagactt
    23581 taactgctac atcacgatcc aagattaaat cgtgcgccaa aaatacattt gccataccac
    23641 cgctaccgat atttccagtg atttgataac ggccatttaa ttttttgccc aactcgatca
    23701 tacactgtcc tccttatatt cgataagaag aacggtgata ttatccgctc cgcctgcttc
    23761 attggcacga tcaatcagct cagttacttt atgagacaat gggccttctt gattgatgat
    23821 cgttcctatt tcttcttcag acagcatgtt cgtcaagcca tctgaacata acagcagtcg
    23881 atcttttagt tgccaaagat gagtagaaac atctacttcg actgttccag gcatgccgac
    23941 agaacgtgtt aaaatatttt tccttggatg gttcactgcc atttcttcgc tgatttctcc
    24001 tgatttgact agttcattga ctaaagaatg atcctccgtc aattgcatga ttttatcttc
    24061 acgaatcaaa taagctctgc tatccccaac attggcaatc gtgaaccagt catctgacag
    24121 agcggccgct acgattgttg ttcccatacc gttatattcc ggctgttctt gtccacgctg
    24181 ataaatcagc atattttctt cttgaatcga ttgaatgaac cattgggcga ttttttcatc
    24241 gtcaacagct tcttgttttt cccaagcttc gcctagattc gttaccgcca tctgactggc
    24301 aatgtctccg gcacgatgac cacccatccc gtctgctaat acagcaagct tgagacctga
    24361 acggctggta aatacactgg catagtcttg atttgtgtta cgtcgtcttc ctacatctga
    24421 ttgatattca atctgcattt tttcacctca ttactttttg cgcaaacaac aaatgaaaaa
    24481 tccatctgtg taaaactgat gcggatacaa agtcaacatt ttctcttcga ttgatgattg
    24541 tagcatagga ttggtcaaaa catcaatcct ctcaaaatca gggtgtcttt ccaagaaagc
    24601 agaaacaacg tcttggtttt cttcttttaa gatcgtacaa gtgctataag tcagaatacc
    24661 ggaagacttt aaagttggtg cgcagctttc taaaatttcc aattggattt ttggtaaatt
    24721 cgctaattca tccggctgtt ttttataacg aatatctggt tttcgccgaa gcagcccaag
    24781 acctgaacaa ggcgcgtcta ctaaaatccg atcaaactgc tctgcttcga actgtttact
    24841 gacttctcta gcatccatct tttgagcgta gacagcagaa gagacaccta gacgtgctgc
    24901 attatcttca atgagtttga ctttgtgttc gtgtacatct aaagcgacca cttctccacc
    24961 gcatgacgga tctaaaaaag aagcaatatg cgtcgtctta cctccgggtg ctgcacaggc
    25021 atctaagaca tgatgttcag gctcgatttg catagacggc gctacaagca tcgaactttc
    25081 atcttggatc gtcatgacgc catcgtggaa caatgagcta gaagctaaat gccctttttt
    25141 cgctactata ccgtatggac tgacttggct tttttcagct gtgatctctt cttcttgcaa
    25201 aacagtgatg gcctgatctc tggaaagtcg gcgtgtatct actcgggcac tagcatggct
    25261 aggctgatat agcgataaac cgagcttttc agtttcttcg tatccgattt gatccaccag
    25321 tcgtttcgtc atccatagag gcaggctgat tttgacagaa agtctttcga taggatcagc
    25381 aatgccttgc agatctggtg ctccttgacg ctgataattc ctcaagacac catttacaaa
    25441 tttccctgtc ccaggatttc cttttacttt tgcgatttcg accgcctcgt ttaaaatagc
    25501 atgtgcaggg actttatcta aaaattctaa ttggtataca gaaagataaa gaagcgtctt
    25561 gacccagtca tccacttttt ttgctttagc gatcaatggt gcaacgtaaa attccaatag
    25621 tcgctgatgg ctaatcgtac catagatcaa ttctgtaaat aaacccacat ctttttgatt
    25681 tagctcgctt tgtttgatca tctcattcaa caaaagattc gagtaggcgc cacccttgtt
    25741 cactcgctct aatgtcagta atgccatata tcgtactgat tttttcattt ttttagggat
    25801 ttttttcatt atttcacctg ctgaccaatc tgaacttgct gaccgcttcc gttaaggaaa
    25861 tcatgtacgg cctgttttcc tttaccagct ggctgtaatt cattgatggc taagacggtc
    25921 tgtttaccgc aagcaatcca tagatttttt ttgtctttcc ggatgatcgt accaggtgct
    25981 ttgtctgtag tttctgccaa tacttcaacc gataataatt tccagcgtat gccctcatag
    26041 gtcgtgaatg caattggcca aggacgcatt ccgcgtactt ggttgtcaat ttcttctgca
    26101 gttttatgcc aatcgattgc ttcttgttcc cgtgtgatgt ttggcgaaaa agttgcttct
    26161 gactcgtctt gaggtactgg tttttgtcca tccaaaatat tgggcaatgt ttctagcaat
    26221 agctttcttc ctaaaaggct aagcttttca aacatcgtcc ctacatcatc ttgtttagtg
    26281 attggtatgc tttcttgcgc atagattccg cctgcatcca tttttttgat catttccatg
    26341 atcgttacac cagtttcttt ctccccattc atgatggcat aatgaacagg cgcacctccc
    26401 cgatatttag gaagtaaaga agcatggaca ttgattgccc ctaattttgg tacttgcaat
    26461 aacttttcag gcaagaattg gccaaatgcc gcagtgacga tcacatcagg caccaattca
    26521 atgatttctt ccatttctgg cgaaccggag attttctctg gctgcagcac acggataccg
    26581 tgcttcagag ccgcttcttt taccggtgtc ggcgtaatta ctcgtttccg cccaactgga
    26641 cggtcgggct gtgttaccac tgcctggatc tcgtaacctg attctatcag cccttcaaga
    26701 atcggcacag aaaaagcagg tgtgcccata aatactagct tagtcatcaa gatgttcctc
    26761 catatattct tcaagttctt cttccgggat ctgctgtatc attttctcaa tgaacaagat
    26821 accgtctaag tgatcgattt catgctggaa ggcacgggct aaatagccaa aagccgtcac
    26881 ttctatctct tctccgtctc gatcataata acgcacggtc acttcatctg ctcgcttgac
    26941 cgttccatag acatgcggaa tactgagaca accttctacg tcaaggcttt caccttttgc
    27001 ttcgatgatt tctgggttaa tcagttcaaa tttctcgcct tcatctactt cgatcacagc
    27061 aattctcttg ttttgtccta cttgaggagc agcgatccct attccgtcat tcgcaatcat
    27121 tgtctcatat aaattgtcta ataaagcgat tgtctcgtcc gtgattacat cgattggctg
    27181 agcagttctt tttaatttat cattagggtg gatcaatata ggatagcgca taatttggcc
    27241 ctttcttaaa taaagtttaa tggttcgttg tcaattgcca caaaaagacc ttgtcgctgt
    27301 tctttctggc ttccattcaa aatttcatcc aaaacatgag gaagattcgg ttcatgttta
    27361 tatttgatga ttagttgata ataatatcgg tttttcactc tcaaaatcat gctgggcgtt
    27421 ggccctaata gcaagctttg agggcttaac acttctttta gctgattggc aatctgaaag
    27481 attttctttg ccgctacttg ttcttcagga tggctggcgg ttattttcac agtaaagtaa
    27541 taaggaggat agcctccacg atgccgtaca tgcatttcct gctgataaaa ctgctcataa
    27601 ttttgttttt tagctaattc tatggcgtaa tgttgaggat tgaatgtttg gatcacgact
    27661 tcgcctgctt tttccgctcg ccctgctcgt cctgaaactt gggtaagcaa ttggaatgta
    27721 tgctcactag aacgaaaatc cggtaaattc agggaggtat cagcattcaa caccccaact
    27781 aatgtcactt ctggaaagtc caaaccttta gcaatcattt gtgtccctaa taatatatct
    27841 gcttcttttg caccgaattt ttgtaaaatc tgttcgtgtg ccccttttct tcgagtggta
    27901 tctacatcca tacgcaaaat cctagcttgc ggataaagtt ctcggagttc ctcttctact
    27961 ttttgagtac ctgttccata gtagcggatt ttattgccgc cacaattagg acagcgattg
    28021 ggaataggtt cttcatgtcc gcaataatgg cagcgcattg aacgtgtatc catatgcagt
    28081 gtaagagaga tatcgcaatt aggacatgga agaacaaatc cacagtccct acacatcaca
    28141 aaagacgaat atcctcgacg attgagcagt aggacagttt gttctttttt gtctaatcga
    28201 ttttgtattt tttcctgcaa attagcagaa aaagtagagg tccgatgatt ctgaaactct
    28261 tcacgcatat ctacgatctc aatagctgga agctgtgcag ctgctttggc tcggtggttt
    28321 aactgcaata actgataaac gcctttttga gctcgtgctc ttgattctaa agagggcgtc
    28381 gcgcttccta gaactattgg gcaatgatga tatttactgc gccaaattgc tagatctctc
    28441 gcatgataac gcggtgtctc atcttgtttg taacttgatt catgttcttc atcgataatg
    28501 atcagcccga tatttttcag cggggcaaaa atagcagatc gtgcgcctac tactacttgc
    28561 gcttcttccc gctcaatctt gcgccattca tcatattttt caccttggga aagtccgcta
    28621 tgtaagaccg caactgattc tccgaagcgt cctttgaatc gctcgaccat ttgcggagtc
    28681 aatgcgattt caggcactaa cataatagcc gttttatttt ctgacaaaac atcagcgatt
    28741 gcctgaagat atacttctgt tttcccgctg ccagtgattc cctccagcaa aaagacctga
    28801 tctttttgct gctgacctgc tgtgatgatt tgttcgacag catttttttg ttctgcattc
    28861 agttccaatg ccgtcgtttg gtcaaatact cggtctttat aagggtcgcg ataacgttct
    28921 gattctataa actccagcca gcccttttct tttccttgat tcaagacagc agtactgatt
    28981 cctttatttt tgaaataagc aattggttct tcttggctca gttcagataa acacaaaaga
    29041 agttcttttt gtttcttggc attttgccgt aattttgccc attcttcttc gatttgcttt
    29101 tcagttaaca aagaacggac caaacgaatc gtttttactt tatttcgtgt atggacttca
    29161 tatttgatat ctactaattg ctcttggcgc cagcgaatca attgagaaag gctgccttct
    29221 tcttctgctt ccttccagga aatcacttct cgctgatcaa acaatacctg aacttctggt
    29281 gtgtctgcca aaggatagat caatttatca tattctgctt tcatcacact cggaagcatc
    29341 gtttgtagac aagtgatttt gaatgcgtaa gtaatttttt tcatataatc agctaatgaa
    29401 agtaattctt cattgaccac cggagcaagg tctagcaaac gaataactgc cttcaaagat
    29461 ggattagctg attcttctga tttttggata gccataacaa atccttggac atgacgattg
    29521 ccattaccaa aagggacttc tactcgcatc ccgacttgca aagctgtttc catttcagag
    29581 ggaaccaaat aagtgaatgg ctgatcggtc tgcatagtag gtacgtccac aatgacttca
    29641 gctgttaatg tcatggttcg tttcctcctt tttttgtctt ttcttatcaa gcgacaaaaa
    29701 gttccttgcc tattttacta gagatcgatg gatttgtctt atcttctgcc aaatcctaac
    29761 aaaaaattaa cagcagataa gaaaaaaaag aactgtgtca aaaagttcag acacagcctt
    29821 ttctaccaga caataagtgg cgaatgtttg aaaaggaaac agctgggttt gcttttcttt
    29881 ttagatgttc gctctatttt tctttattac attttttgct cttcacggat acgtgattcc
    29941 aattcgtgtt gttcacgctc tttttgtgct tgacgttcct cttcttccat cttcaaacgt
    30001 tcacgtttta attcaggatg tggatcattg actacatttc ctgcatcgat ttcttctaaa
    30061 gcttgaccga cgctttttac tgaatcaaaa gaatctagag taggctgtgc acttgcatcc
    30121 aattcatgcg cgcgtttact tgctaaaatc actagagaat attttgaatt tacacgatct
    30181 aatagtgagt caattgaagg ttttaacatc ataagactac atctccttta acattttaat
    30241 atatttaccg attacgcgat ctactcgaaa atgttcactg gcaataatgt ttttgattcg
    30301 ttcaactgct agcggcactt catcatttac aacagcatag tcgtaaagag ccatcatttc
    30361 gatttcttct tttgcaactt tcattcgttc ttcgattact tcatctgcat ctgttcctcg
    30421 gcctacgatc cgtgatttta attccgctaa atctggcgga gtcaaaaaga tgaatacacc
    30481 atcgggcact ttttctttga cttgttttgc tccttggact tcaatctcca agaaaacatc
    30541 ttttccttca tccaatgtct tgttgatata tgacaaagga gttccataat aattcccgac
    30601 atattctgcg tattccagca tctctccagc ttcgatcatt gattcaaact cttctttgct
    30661 tcggaagaaa taatcaacgc cttcgacctc cccttcacgc attttacggg tagtcattga
    30721 gatcgaatac tgaaaatcat tatcctcact ctcaaaaatt gcttttcgta ctgtgccttt
    30781 tcctacaccg gatggaccag acaacacgat taataatcca cgctctgaca tgacgacgtc
    30841 ctttcaacta gtctttttgt acttatcgtt tattttgcac tttttttctg caactttcaa
    30901 ggttatcttt aattatagct agaaattggt aaaaaagcta ccctgtacca agaggtcgta
    30961 aaaaagctgt cctgcatcaa gtaataagaa cagccaaaca taaacagctt ctcatgtttt
    31021 tcgtttggtt ggggttatta tcgcagatgc aagcttttga acgccgtgta taagaggtcg
    31081 tgaagaagcc gattagtttc gagtatgaag aaaaattttg tgaaaatagt ttctcacatt
    31141 tttgcataaa tttggcttaa ta
//
LOCUS       DACEXQ010000009        30943 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.009, whole genome shotgun sequence.
ACCESSION   DACEXQ010000009 DACEXQ010000000
VERSION     DACEXQ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.009"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(133..1701)
                     /locus_tag="IXH04_000306"
     CDS             complement(133..1701)
                     /locus_tag="IXH04_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAQ1850941.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(1705..2115)
                     /locus_tag="IXH04_000307"
     CDS             complement(1705..2115)
                     /locus_tag="IXH04_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAQ1850942.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(2112..3035)
                     /locus_tag="IXH04_000308"
     CDS             complement(2112..3035)
                     /locus_tag="IXH04_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAQ1850943.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(3032..3910)
                     /locus_tag="IXH04_000309"
     CDS             complement(3032..3910)
                     /locus_tag="IXH04_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAQ1850944.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(3897..5234)
                     /locus_tag="IXH04_000310"
     CDS             complement(3897..5234)
                     /locus_tag="IXH04_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAQ1850945.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(5237..6340)
                     /locus_tag="IXH04_000311"
     CDS             complement(5237..6340)
                     /locus_tag="IXH04_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ1850946.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(6414..7922)
                     /locus_tag="IXH04_000312"
     CDS             complement(6414..7922)
                     /locus_tag="IXH04_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ1850947.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(7888..8580)
                     /locus_tag="IXH04_000313"
     CDS             complement(7888..8580)
                     /locus_tag="IXH04_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ1850948.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(8602..9576)
                     /locus_tag="IXH04_000314"
     CDS             complement(8602..9576)
                     /locus_tag="IXH04_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1850949.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            9876..10748
                     /locus_tag="IXH04_000315"
     CDS             9876..10748
                     /locus_tag="IXH04_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1850950.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(11206..13404)
                     /locus_tag="IXH04_000316"
     CDS             complement(11206..13404)
                     /locus_tag="IXH04_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1850951.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            13636..14628
                     /locus_tag="IXH04_000317"
     CDS             13636..14628
                     /locus_tag="IXH04_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1850952.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            14688..16109
                     /locus_tag="IXH04_000318"
     CDS             14688..16109
                     /locus_tag="IXH04_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAQ1850953.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            16102..17601
                     /locus_tag="IXH04_000319"
     CDS             16102..17601
                     /locus_tag="IXH04_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAQ1850954.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            17640..19034
                     /gene="uxaC"
                     /locus_tag="IXH04_000320"
     CDS             17640..19034
                     /gene="uxaC"
                     /locus_tag="IXH04_000320"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAQ1850955.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            19193..19876
                     /locus_tag="IXH04_000321"
     CDS             19193..19876
                     /locus_tag="IXH04_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAQ1850956.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            19878..20714
                     /locus_tag="IXH04_000322"
     CDS             19878..20714
                     /locus_tag="IXH04_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ1850957.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            20725..22479
                     /locus_tag="IXH04_000323"
     CDS             20725..22479
                     /locus_tag="IXH04_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1850958.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(22755..23096)
                     /locus_tag="IXH04_000324"
     CDS             complement(22755..23096)
                     /locus_tag="IXH04_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ1850959.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(23164..25335)
                     /locus_tag="IXH04_000325"
     CDS             complement(23164..25335)
                     /locus_tag="IXH04_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ1850960.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            25546..26403
                     /locus_tag="IXH04_000326"
     CDS             25546..26403
                     /locus_tag="IXH04_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ1850961.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(26474..27331)
                     /locus_tag="IXH04_000327"
     CDS             complement(26474..27331)
                     /locus_tag="IXH04_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1850962.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(27316..30018)
                     /locus_tag="IXH04_000328"
     CDS             complement(27316..30018)
                     /locus_tag="IXH04_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ1850963.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(30031..>30943)
                     /locus_tag="IXH04_000329"
     CDS             complement(30031..>30943)
                     /locus_tag="IXH04_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAQ1850964.1"
                     /translation="WIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQSFIEGIKKIL
                     DVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRPSDDTCRYGYL
                     VPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRTVNQKGQTIYA
                     YEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKENPYFYEGKYAK
                     GIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMHEGFDVNNPEN
                     YTREWFSWANMMFCELVMDYFDIRVEK"
BASE COUNT         9054 a         6406 c         5522 g         9961 t
ORIGIN      
        1 aaaaacaaaa aataatctaa aaacattgat gcataagatt gataactttt tttacataac
       61 aatatataca aacaaaaaaa ttcactaact agtgaaaata agttagtgaa cctcatttat
      121 taaagaatta aattacagat gatcttttcc atcatacttg aaaaactcaa aatcagcata
      181 acttcctctt tttttattta gatcatgcac tgctatcccg ataaaatttc cagtaaaccc
      241 tcctgagagt atacacatgt ttttttgcgg acctactttc ttccattctt cagttgatac
      301 gtctttcaaa tgaaactgtg ctgtctcttt ctttacagtg actttcaaaa aagtcttctt
      361 ttctgtgagt ggaatcgtcc aatcttctat ttgtgctact ccgttttctg attctatcat
      421 acgtaggcat tttcccttgt cctcattcca agtaacataa caataaatat aattggattc
      481 atttaagtac agtaccagtc cagccatctg attaaaatta ttaggcatgt attcaaatgc
      541 tgtctccgca tcaaaagtaa aatccgtttg ccgaatagct aacaaatgat gttcgaaggt
      601 agattgaaga gattcaccgg aaatcagacg taaaaaacct ggtctacatt ttgtatcaca
      661 ccaatcagca tttggcaaaa tgcgtctggc attccagttc ttctttagct ctccttcaaa
      721 tgtatccata aactgatgat ctttttcttg ttgtacctct tcatttgtct caatcattat
      781 ttcttccatc ggagttgtct ggctatcttt aagtcttaac caatcgtcgg acgtccaata
      841 cacttcttga atagcggttt ctcttcccaa aatagccgca ccgtttaatg gtctggtaca
      901 aagatatgtc atgtaccatc ttcctgacgg ggtttgtact aagctcccat gtcctgtaca
      961 ttgcaatgta gaagcaggtt tgtcactagc tgtcagcata ggaaaaccag gatcaagctc
     1021 gtaaggacca gtaatattct ttgctcggca aactgtaact gaatgtcctc ttcctgtacc
     1081 gccttctgcc gtcatcaaat aataataccc gttacgaaaa tacaaatgtg gtgcctccgt
     1141 tttcgctaat tctgtacctg caaatatttt atagatggga ccatcaagct tcttcttctc
     1201 tgcatcatat tcttgtaata cgataccgct ggatttattc ggtgtgtcca gacgataatc
     1261 ccatagttca ttgacaagcc atttttttcc cgtttcaaga tcatggaaca aagaaggatc
     1321 aaagccgtta ctatttaaat aaattggttc ggaccaagga cctgtgatct cagatgcagt
     1381 gatcaaataa ttgtggacat ctttaaacgg acggcctgca cttttgacat ctgtgtaaac
     1441 aagataaaac aatccatcag cataactgat ctgtggcgcc catatacttc catttgtagg
     1501 atttccttgc aaagatactt gagtggtcaa aatatccgtt gtatgtgacc agttgactaa
     1561 atttttcgac tcatatactc ggattcctgg aagccattca aaagaggaga caataatgta
     1621 ataccgctct ttcactctta atatattagg atctggattg aatcctctta aaataggatt
     1681 tttcactttg atagtctgca tgattcaggc tccttttctc attcttactc gatataaact
     1741 gaatgcccca gctgcgaaat aaaaaaatgc aaaaggaaac gcaataaact gagtgccaat
     1801 caacaatatg aatcctgtta taaaatacaa accagtggca gttgttaagt attttccttt
     1861 agcagtcaaa aacaatgcta ctgctaaaaa agcaagtgaa ataaacaaca caacggcaaa
     1921 cagaccagtc atgtcctgta ataatccaaa tgtattttct aaagacaaag ttgaatgatc
     1981 tacagcaagc gactgataac tttttttaaa ctctgacgca gacatattac ttaaagtaac
     2041 tgctaacccg ccaaagaaaa tcaaagatag actgccaccg accattccta gcaatagttc
     2101 ccattttctt ttcatttctg aaccccctgc ttttctaaat gtatccattc aggtcttttg
     2161 atcgatgttt cataattgtt attacgttca gagaatttga cagtctgttc atttttcttg
     2221 tcatcccaat tatcccaacc agttgggtga acatgcccac ctatcttaca cgcttcaaat
     2281 aagacatttg catacggacg ccacggtctt cctaaataga cgtttctagt atcaggttct
     2341 gcagtcaagg tacagctttc aaaacgatac cctgtttttt gatttttcgg cgtagatgct
     2401 gctgtgatgt atccaggatt tttactggct aagctatgga tatgacactg atgaaaaacg
     2461 gcatctgccc caccaaatat gaaatccacc gttccttcaa tccagcaata ttcataggtc
     2521 tgacgacaat actcaggatt ggcagaagcc ggtgtacaaa aaggagtacc atctttttgc
     2581 aaatcaggca acggacccgt gcataaggta tcctgataac cagatagtcg acagtttttt
     2641 aaagatacgc gatctcctga attgaataaa gcaacagcct gtcctacctt ttctcctgga
     2701 cctgctgtat tttcgatgtg aagattttct aatacgacat tcgatccttc taagaaaaaa
     2761 gtagcacttc ggaaagtccc taactggcta ccgtcaagat actcctgttt tgcaaaacga
     2821 ttaccggaga taactactgt tcctgctccc acaagtttaa aattggataa tctgctttct
     2881 atatattcat catatcttcc ttcttttact atcaagacct ttggatggct atccggcagg
     2941 cctgccaaat aatcgattcc ttcttggata gtttcaaaat ccgtcggggt atctatagca
     3001 ttctttccaa ttatcagttt gtctttcatc ctcataaatg ctcccttatg aattgtttcc
     3061 aaatatttct catttcttgt gtttccatat gaccgcctgt taaagaaaaa gaagaaaagt
     3121 tttctggact acccattgat tgatactttt tgtccagccc ttttctcagc ttcagtacgc
     3181 cttcgtccaa gcacatcgta tcgttttttc caactaagct aagacgaggg cgcggagcga
     3241 ttttttcttg gattgctaac gttgtataag ctgtcaatag atttggcaca tagtaataaa
     3301 acccatgatg atctaggcgg cgatgtagca aaagtatttc gatgtccacc tgtgccgcta
     3361 gatcaataca aactttgaca cgagaatcca atgctgtcac ccaccagctt aataagcctc
     3421 ccatagaaag accaatcgtt gcgagccgtt gagtatctgt gtcttccctt gtctccaaat
     3481 aatcgatcaa gctgatcaca tcataaatac gcattcccca taacgttttt cctgttaata
     3541 aaaattgttt gaacaactcg ctttcagaaa tacctccgcg ttcttcaaat ccccaagcat
     3601 caatcgacca aacagcatat cccattcctg tcagtacttc tgcaaaagat ggagaagcta
     3661 agtaaggagc accatggatc aattcacttt tcccgactga aaaatctccg ccgtgagaat
     3721 gcaggtaaag gaccggcgga aacggtcctt ctttttctgg ataagcatag agggctggta
     3781 ctgtttccaa ttcatttaag tacaattgat agtattcaag cataaaaccg ttttgttttt
     3841 ctcggtataa tcgttttcca ctaggggtat gtctgctaga tggagcacct aaaaaatcat
     3901 tcaactgcat aagcaaagtt tttacgaaca accagttcat ccatattttc tgattgattt
     3961 gattggaatt tctcaatact gatttcttta gtattttcta atagaaatgc tggtccctcg
     4021 tgattttcaa tcgacatctg gttaaaggaa accttacccg caaagccaat ataaaatcct
     4081 tgtctgctca tttcctctat tcctgtcatc atagcaggaa caccgggaac tgcatcttgg
     4141 gccatcgaga catcgatatt gtcaaatgtg atttcttgta tgaattgttc cgctaatcca
     4201 taaataaaac ctgcagaagc atggacattc cttgccgtga tattcgaaaa atggatcctt
     4261 ctgaatgctg gtgtccgttc atctatcgga taagcctttt tttcccatac atacggttct
     4321 ttccctcgcg gaccacagaa ataataaaga ttcagtataa atggacacat cacattatcc
     4381 atgactatat tggatactcg aatatcttca acgatcccgc ctcgacctcg tctagatttc
     4441 aatcgtattc ctcgatcagt ttcttggaag atacaatttg aaatggtaat attacgaata
     4501 ctaccactca tttcacttcc taatacgaca ccgccatgtc catggaccat cgtgcaattt
     4561 gtgatcgtga tattttcgca agcgatcctt tcgtacgtat cttcagtccc tgccttgatt
     4621 gcaatacagt catctcccac gtcaatatgg caattactaa tacgcacatt tttgcatgat
     4681 tctggatcaa ttccgtctgt atttggtgaa tccgctgggt tcaagatcgt caagttatca
     4741 aacgttgcat tactgcaaag aatcgggttg atcgtccaac ttggtgactg gatcaattta
     4801 atatctttca cagtaattcg atgacagtta tggaaactca tcaatttagg acgaggataa
     4861 gcgagattat ctggttcatt tctaaaagta tgccaccatt tttttccatt tccatctaat
     4921 gtaccgaaac cagtgacaga gatgttctct acattttgag catagataca ggaagcataa
     4981 acttttcgat ggactccttc ccaacgtgaa tgaacaacag gataatcttt tggatcgtca
     5041 gagaatttca gaaccgctcc tgcgcttaaa tgaagttcga cattgctttt taaaaataaa
     5101 gctcctgtta gaaattcacc tgctggaaca actacggtcc cgcctccgtc acttgctgca
     5161 gcatcaatgg cttgttgtat tgcttcagta ttcaattcat caattgatgc gccaaatgtc
     5221 aaaatatcat acatatttat aatgcctcca cttcaatcaa tgcttgcaag aatgcaccaa
     5281 cgcctttttg atcattacaa ataattggtt cactgatgta gtaagcatat gaaccatctc
     5341 gctgatctgc tccgcctaac cctgctactt gacagttttt attcaagttc agccagcctt
     5401 cttttgtttc cagaacaaat tcatcaagta atccttgatg tgatttcttc acttgtggaa
     5461 gccattcttc tttgttcagc agtcccaaac gtattccttt agccattgcg taaacaaaca
     5521 tactactgcc ggaagcttcc agataatttc ctttccgttt tgtttctgtt gttacttgat
     5581 accatgtccc gctgttttct tcttgatatg gaagtaatgc agccagtgtc ttttctagaa
     5641 tttctttcag caaagaacaa tctgtttctt catgaagtaa ttcataggta tctgctgcag
     5701 ccatcaaata ccagccaatc gatcggctcc aaaaatgatg ggatagacct gtttttggat
     5761 cagcccatgg ttggaccttc ttttcatccc atgcatgaat cagcaaattt gtttttggat
     5821 cgaatagatg tttataactg atttcaaact gtctcaaaac atcttggtaa tctttttctt
     5881 cactaaaatt ctggataaat tcagcataaa aaggagaacc catatataag ccatccaacc
     5941 aaatttgata aggataaatt tctttatgcc aaaaagctcc ttcggatgtg cgtggatggt
     6001 ttgccaactg ctgggaaagg agttctgctg cttttttata tttttcttcg cctgtttctt
     6061 tgtacaataa aaagaaaagc tttcctgtat ttacatggtc gatattgtat tcttcaacgc
     6121 tgtagccacg gatcgttcca tcttcttgca cgaaatagtc gatattgtct tttatgaaat
     6181 caaaataaat tttttcttgg gttcgttccc aaagtaattg gaacccttta agtacgacgc
     6241 catagtcata agaccatttt cctttatacc acttttcttc atataagcga ggtgttcttt
     6301 ccatgatact ttttgctaat ttctttgacc agttttccat tatttgctct cctttaaaaa
     6361 ttaaaatagg atttgggaca aataccaaga agtatttgtc ccaatcgtct atattactgt
     6421 gccgctgctt tgtatgcttc ctcgtactct tgtgtgattc gatcgcctcc ttgtgctttc
     6481 cagtcttcta cagctttttt gaatccatct tcgtcaagct gtcccatgat gtattgcacc
     6541 gtagcatcaa tgacgatttt ttcaatttct gatcctactt cattgttcgt cggtgaatcc
     6601 aatggcacag aaggatccat tacagcaaat tcttcatttt ctttgatcaa ttgattagag
     6661 aattcttttt cagggtctgt atctttgaaa gagaacgtca cttcactagg acgagagctt
     6721 gataacggtt gcacctcttg ttgccaaaga tccatatcga taaattcgat agaaccatca
     6781 tcattcaatt cataatgcgt tccttcgata ccgccagtca tcaatttata cacatcttct
     6841 tctaaaagat catcaacaaa ttgaagtaat ttcttcaatt gtgcttcgtc ttttacttga
     6901 gatttgggaa atgctagcaa gccgccaata ccatttcctt ctgaccaaac atgataaccg
     6961 ccgccttcac catctgagat cttgtttaca ggcactaatt ccatatcatc ttgaatacct
     7021 tgacccatcg tcttcagatt cttgatatcc accattccag tgtagattcc agttcttcct
     7081 tgtgcaaatt gttcttgctg atcagtcttt tgggtaactg caaagtcttg tgccaaatag
     7141 ccattctcaa atagatctct tgaaaaattc aaagctttca tatactcttc tgtatcaaat
     7201 tctggagtga atttgccatt atcgtctaca gcccagccat ttggtgctcc aaaataagaa
     7261 gtgaaaagtt taaagctggt ataacgaata tcactaggcg cacgatcgcc aaaaccaact
     7321 gtgtcgtcca caccatttcc atcaggatcg ttttctgtaa attgtttagc tacttcataa
     7381 agctcatcca tggtagttgg aacatctagt cctagattat ccaaccagtc ttttcgaatg
     7441 actaagccag cacgagcgta gtctttttgt atcggtacgc cgtacaaggc accgtcgata
     7501 ctcgcagctt cgattctatc ttcagaaatt tgacttaaat ttttatagtc ttttaaatat
     7561 tctgatacat cccagaatac gcctgatttt aacgcattgc ggacagaaga gtttttcaac
     7621 atcgcaattt gaagtgtaac gatatctcct agttcatcag aagccaatgc agttgttaat
     7681 ctttcttctt ttgaagcatc aggaatccag ttgaatgtca gttccgtatc ggttttttct
     7741 tcgatcaagt tcacaatcgt gtctgtaggc ggtgaagcat gatgcaaaat atttaaccaa
     7801 gtgattttag cttttccatc ttctgtctct gaagaactgt tacttgaact gctgccgcat
     7861 gcggcaagtg ctagaattcc tgcaaaacta agacctaatt ttacgagcgt tgcttttttc
     7921 ataaattttc tccttcactg ttttaataat atttttgttt tcttctgttt tttctgtata
     7981 tgagttcttc cattccaaat ggcaaaatat agaatttgct gtccacgtga ctgcggaaaa
     8041 gaagagactg ccaccgataa aggttaaaac acctggcatt gtttggatga tcatcagata
     8101 aatccctacc atacttaata tcaacactaa tgagtaatga agatgtgaga atccgaataa
     8161 aaatgccatt ttcattttga aaaaatatcc tttccaatca taatgagcca tgattgggaa
     8221 aatatagagt gctgagagca catatatgaa cgcagcaata tacaaagcga tcctaagata
     8281 ccaattgtgt atgaaagaaa gatccacaag taaaacaatc ccgatggcgc catatatcca
     8341 gctcatcagc aaggtttcac gatagttttc tttaaaatat gtgaagtacg ttttcgtcaa
     8401 aggaaacgtt tcttttgagc gcagccattt tctaatcact gcaaccatcg catacgatgc
     8461 aggaccgaaa gtaaaaactc ccaaacctgc gatgactaaa ccgccccaca gaaaattcaa
     8521 ataaaccaac tgtaaaatac gatttagcct tccgtttatt ttatctaatg actgcagcat
     8581 atctttccct ctctttctat cttagtaaac gccgtcttct cctagctttt tggcaatctt
     8641 attagctaaa attaccataa acaatcctac gaccccttta aacaatccca ttgctgtact
     8701 gaaacttaac tgtccattct tcaatccagc tgtataaata taggtgtcga aaatttctgc
     8761 aacgcttcga ttcaatgaat tcaataacaa gtacatatgc tcgaacccta agtctaatgt
     8821 ctgaccgatt tttagaatca gcaaggtaat gattactgga cgaattgccg gcaatgtaac
     8881 atgccatgtt ttgcgtaagc gtcctgctcc atccatttct gcagcttcat acaactgtgt
     8941 atcgactgca gttatcgctg aaaggtagat gatcgtagac cagcccaact ctttccagat
     9001 cacttgtccg atatatactg tgcgtgtcca ttcaggggat gttagaaaac tgattttttc
     9061 aaatcccatt gttgcaagga attcattcaa tacccctccg tcaacattca aaaatacata
     9121 agtaatcgaa acaatgatta cccaagacat gaagtgaggg atgtaaatga tcgtttgaat
     9181 cccgtttttc aatcgtttgt tcgttacttc atttaatagt aaagacaaaa tgattggcat
     9241 cggaaaaaac acgaagatat ttaatccgaa caagatcaac gtatttctta ataatgtaaa
     9301 gaatgtcggt tctgtaaaca aacgaatgaa atgtttgaaa ccgacccaag gacttcccat
     9361 tattcctaaa aatggttgat aatcttgaaa agcaataacg agtcccccca taggaagata
     9421 cctaaaaatc aagaaataaa ggactcccgg cagaatcatc acatataaaa atttattcgt
     9481 tttgattcct gcaattattt tttttcgtct tctgagcttt tcttcttttt tcgatagcgt
     9541 cagttgaaat ggttcttgtt tctttacact ttccataagc ccctccttag ttttgtgaat
     9601 gtttgctgct tatgacttca tgatagataa ctatctgaat tacgtatata atcattttta
     9661 ccgtaagtaa aaacgcttac atagcaatga tttttgtgct atacaaaacg ataaagtgta
     9721 ttttttcaca aataaactac gcatagcaaa ccttctattt ttttctatct gacacttagc
     9781 ataggaaatg attatatacg taatctttca ccgatgataa attgaacata tagaaacaga
     9841 aacatttaat ttattttgag gaggagccta tctatatgaa aaagaaagtc tctataggag
     9901 aaagaagttt tacacttttc aacaatacgt ttctaatttt gctagctttg atctgtatcg
     9961 ttcctttttt aaatatcatc gctacatctt ttgcttctac acaagaagtc gtcgcgaaaa
    10021 agtttatcct ttttcctact accttttctt tagacgcgta tcgctacatt ctttctacgc
    10081 cgaccatttt tagagcactt gctgtttcga ttggtgttac aggtgtcgga acgatcgtca
    10141 gtatgtgtgc aacttcactg atggcctacg gtctttccag aaagtatttg tttggtcgag
    10201 gtttcgtcaa ctttcttgtt gtattttcta tgttgttcag cggggggatg attccaacgt
    10261 tcttagttgt tcgttctcta gggctggtca attcttactg ggccatgatc ctgcctgtgg
    10321 ctgttaatgc aatgaatatg atcattatga gaaacttttt ccaagccttg ccggatagtt
    10381 tggaagaatc agcaaaaatg gatggttgca cagattttgg tgtatttttc aaaattatgc
    10441 tgccattagc gttaccgtct attgctacta tctcgctttt ttatgctgtt acttactgga
    10501 acacttatat gactgctatt ttgtatatca acgattcatc taaatggccg atacagattc
    10561 ttttacgcca aatcgtcatt gtatcaagtg ggatgcaagc agaaagtagt gccgttgatg
    10621 tgattccacc agcacaaaca gttaaaatgg ctgttatcgt tattgcaaca gtgcctatgc
    10681 tgattgttta tccttttgtt cagaaatatt ttgtcaaagg agcactcgta ggttctgtca
    10741 aagggtgatt atgtactgaa cgcaaaatgg tagttgtgtc agaagtggtc agcttcaaga
    10801 aataagaagg catttacgaa aattgcttct taaatttttg tgaaatttcg gcttattttt
    10861 cgaagaatct actcctggaa caccgtgtat cagaggttgt gaaagagacg ttcagccttg
    10921 agcgatgata aaaactctca caaacattgc gacgagtaac ccacaggagc aataagaagc
    10981 gattttgaaa aatagcttcc catatttcac aaaattatgc cttattgtcg atagcctgtc
    11041 tcttgaacgc catttattcg taaaaaagaa ctgtgacaag acttttgtca cagttctttt
    11101 tgcgtgctta gccttgaaat ttaactatcc ataaatacaa atactagggt tattctaact
    11161 tcttttattg cttgaatcag tccacaagaa aaacagcctc ttgattcaca aatatttttt
    11221 tcgatattct cccggcgtaa tcgattcctt ctttttaaag aaacgaataa aattttgcgg
    11281 atttcgatat tgcaatcttt ctgcaatctc ttttacggag aggttagtct ctttcaacca
    11341 tttttttgct acctctaaac gatgattttg aacaaaatca ccaaattttt ctcctgtttc
    11401 ctttttaaat acattactta aatagttagg gttataatgc aacctatcag cgatcacatc
    11461 taaagacaga tcacgatcat attctgtgta gacgatatgc ataatattct ccgacaaatt
    11521 tttaaattca tgatcagtga ttgcttgaga gcattctgtg attggcagaa ctaattgttc
    11581 taaaacaagt ttttctagtt ctttgggatg ataattgttc agtgctttta cataaatttg
    11641 tttcatgtta tcaaagtttt ttgaatctac tcccaataac tgtcctaatt gaacgagttc
    11701 attgatcaag cggaccgaaa ctacttcccg actcaaagga tttttatttt gtgcaaacaa
    11761 tgcatcaatc aattcatgta ctaaattctt cacttcctga tttccagaac gaatggcttc
    11821 aaacaatcga ttttgttctt ctatcggata tctaataagt gttttttcat gagcagcagg
    11881 aacgatttcc tgataaaaaa taattgaact tggaccagta tttacacgat aatacagggc
    11941 ttcttttgcc cgatcaaccg attgcttgct ttcattcagc gtttcgaacc ggttactgat
    12001 gcctatactg atagttaaat tcaagtattc ttttacacgt ttttgaatga gtcggcaata
    12061 ctccatgact tttttgcttg cttctgcatc atctttatcc attcgataaa tagtggcttg
    12121 catttcttca ttcaaaacga tcggcagcat tcgattttcc tttggcacaa tttccgtgat
    12181 catgttgttg atcgctaaaa gtagcaaatc acgctcccca ccgtgactat cttctaacag
    12241 atcgatttga accaaagctg tataatagca gtcattttga gaactataac caaattgctg
    12301 caaccgctga tctagttctc gcctttctac tcgatttcta aataaactaa gtacaaatag
    12361 cgtttccagc tgtggctttt gggtaactaa gtgtgcactt aacgcttctt tttctcctat
    12421 gatccgactg actgattgag cgaccaaatc tagttctttc ccactgaatc cttttgattt
    12481 aagattcaga cgttcctgta tttgagaaat cggacgggca aatctgtccg aaaataagta
    12541 ggaaagaaga ccaatgagtc ctaccagtgt taaagacact gcaactagcc cgattctcag
    12601 attgcggatc attgcgccaa ttgcactaga gtcaacttct aatctgtaaa tccaattatt
    12661 ataatctgat ttcagtacta tatacgtttt atctttttct cttatcactt tcagctgatt
    12721 gtaatctaga tccacgtccg ttaatacagt tggttgctct ttatttttta ctgtctgagt
    12781 agtaaaaagg ttgccaactt cgttttcaat agaaagtatg tcttctccac tttcaccaac
    12841 aacttgttct attgtatgtt tctctatcgt agcaattccc aatgcatatt tttctttttc
    12901 aaacattggc aaagaaatca ccatttcgat tcctttgttt ttcttttgcc aaaataaatt
    12961 atttttgtta tttatgtact tctgttgata cttttcaatt tcctcttcgg tcagttgact
    13021 caacgatcca ttgattactc cccatttacc agccaaactg actaaatcat aattattatc
    13081 atccataaca atcgtttcta taaaatttag ttctttcctg atttcagaat aagtggagta
    13141 atctgtatat gtcagcggat gcttgaatat gtccgagaaa tttttggtcg aaccatacgt
    13201 agaaaacgaa tattcaatcg ttctaatttt ccattcaacg ttagttttaa cttgcctgag
    13261 caaattcttt ttgtctgtta aataactcga ttcgatcgat tcataggtat tctggtaaat
    13321 gaataaacta aatcctatga caagtacggc tcctaataaa aacatcggta aaaatatttt
    13381 ataaaatact gtacttttct tcattttctt cctctcctcc ttttaaaata aaaggctttc
    13441 atttttctgt aaaattgata ctcatctaat taaatgagca ttccccgctt tcaagaatag
    13501 cataattaaa aataaaaatc caagtaattt ttaacgtgtg caatcttttc atatcaaaga
    13561 tctattgact tatcgtagtt atcgtaatat gatgaatgcg ttaacaaaaa gtgaaaaagt
    13621 aaggaaaatg atggaatggt aacgatcaaa gatgtcgcta aagcagctgg cgtttctcat
    13681 acgacagttt ctcgtgcttt gaataacagc agtttaataa aaaatgaaac aaaacaaaaa
    13741 atacgacaac tagctaagga aatgaattat tcgccaaact atagtgcaaa aggtttagtg
    13801 aatcaaaaaa aatatctaat cggactattt ttctcaagtt tgcataaagg aacttcatca
    13861 aatttcctag cagattccat tgcaggcatc cattcagtat tagacacaaa ttacagttta
    13921 tctgttgaaa gtatcgatgt tgttgattta acagaaatca acctgcaacg ctacgatgga
    13981 ctgatcgtta tgagtcaaag tgatgatgat cagcctttca tcgactatct caaagaaaaa
    14041 gaatttcctt tcgttgttgt caatcgtcac atcaatgatc cagcggttat caacgtcact
    14101 gccgatgatg cggccggtgt tatgcaggca gtagattacg gtattggatg cggacatacg
    14161 aagatcgctt atattggcgg aaagaaaaat tttcgttcga caaaagaacg ggaaaaaggg
    14221 gtattgaaaa gtctaaaaaa agcgagcatc cctgtcaatc cagcctattt tttaacagga
    14281 gattacagct tagaaagcgg cctccaaaac atggaatacc tattatcact ttctgacctg
    14341 cctactctgg ttttttgcgg caacgacgac atagcgattg gtgcattgag agctgtacat
    14401 ttagctggcc tccgtgttcc ccagaacatt tctctgatcg ggttcgatga ttctccagtc
    14461 gtctcctatc tcaatccgcc gcttaccacc gtacgtaagc ctttgaaaga aatgtgtcaa
    14521 gaaggaacaa agcttttact agatttgatc gaaggcagga cgatcgaaca atctaaaatt
    14581 gagctctcca ccttgttgat ccttcgtgag tcgatcaatt acttgtaaac tacaagtaat
    14641 tttatataaa aatgttaacg tgtgcataac acagaaagga agtttatatg aaaaaactga
    14701 caaaagaatt agtgaaaaaa aacgaagcac ctgtaaaagt tcttcagttt ggtgaaggca
    14761 attttatgcg tggatttatt gattggcaga ttcagcagct gaataaccaa caattgttta
    14821 aaggtaatgt tgcaatcatt caacctttag aacaaggtct tggaaatatg atgaaccagc
    14881 aagataattt atatacggtc attttacaag gactcagcaa tggcgaaatc gttgatactt
    14941 ccgagattat caccgtagta gaacaaatga tcaatcctta tgatgaatgg gatgcttatc
    15001 tatctctagc agaaaatgac gacttggcct atatcgtctc caatacaaca gaggcaggaa
    15061 tccaattcgt ggagactgat aatttaacag atactccccc acagagcctc ccgggcaaac
    15121 tgacagcctt tttataccga cgtttcaaat taaacaaagc tggctttacc atcattcctt
    15181 gcgaactgat tgacagaaac ggcgaacgtc taaaagaaat cattttacgc tacgcagaaa
    15241 aatgggattt agaacctgct tttgttgagt ggttagaaaa agaaaatgtt ttctgttgta
    15301 gtttagttga tcgcatcgtc ccaggttatc ctcgcgaaga tgcaggtgtt ttcaatcaac
    15361 ggcataacta tgaagatcaa ttgatggtca aagcagaacc attcatgctt tgggtgatcg
    15421 aagggcctaa agaattggag aatcaactac cattcaaaca ggctggcttg aatgtcatca
    15481 tcacagatga tatgactcct tatcgtgaac gtaaggtgca tttgcttaat ggaccgcata
    15541 cagcgatggt ccctcttgct ttgttggcgg aattggaaac tgtagaagaa gtaatgaaag
    15601 atcctttatt ctccgcatat gttgatcagt tattcaatct agaattgatt cctatgcttt
    15661 ctttacctaa agatgaatta gccatttatg ctgaccaaat caaagaacgt ttcttgaatc
    15721 cttttgccca tcacaaatta gaagcgattt cattaaatag tgtttcaaag ttcagcaccc
    15781 gtctgcttcc agtgttcaag aagtatatcg aagaacaaaa tcaggtacca ccgttaatca
    15841 ctgtttcact agctgcgcta cttcttatgt atcgaggcga tcaagtaaag ccacatgatg
    15901 atgaaaagac gataagcgaa tttactgatg cttggtctga taatggaaca gctattcctc
    15961 gattattgca aaacgctgcg ctttggggag aagatttgtc tcaaataccg aatgtgacag
    16021 atacagttca agaaatagct gcaaagatcg atcaaaaagg tgtccggtca gtattacaag
    16081 aaatgaaagg aacgatggtc catgcttgaa accctgcaac caacaatgaa attaaatcga
    16141 cgagacagtg tggtcgtcgc attaacccct attccaaaac aaacacaact gacgattgat
    16201 cagcaaacga tcaccactct tgaagatatc ccgcaaggtc ataagattgc attgggtgat
    16261 ttgaaagctg gagacaacgt gatcaaatat ggctatccaa tcggtcatgt gacgacagac
    16321 gtaactgccg gtcaatggct gcacactcat aatgtaaaaa ccaatctttc tggtgaattg
    16381 gattatcgat atgaaaaaga tgtccatcca agtcattacc tttttgagaa ccgaacattt
    16441 caaggttact tgcgaaaaaa tggaaaagta gggattcgta atgatttgtt tttagtacct
    16501 accgttgggt gtgtgaatgg cattgctgaa ctgatcgtca aacagttcaa ggaaaaacat
    16561 ccagatctgg gacagtttga ccatattacg attttaaagc atccttatgg ctgttcgcag
    16621 cttggaaaag atcatcaaaa tactcgagaa attttagcgg acgcagtaaa tcatcccaat
    16681 gccggaggag ttttagtatt tggattaggt tgtgagaaca atacagttcc tgaattcaaa
    16741 aaaattttag gtgaatatga tgaagaacga gtgaaatttc tagtggctca agatgtttac
    16801 gatgagatcg aacaaggtgt ggctctatta gaagagcttt taacagcggc ggaaaatgac
    16861 cagcgcatat cagtcccgct ctctaagttg aatgtcggtt tgaaatgcgg cggttctgat
    16921 ggtctatcgg gaataactgc taatccttta cttggtgcat tttctgacta cttgattgct
    16981 caaggaggca gcacgatctt aacagaagtg ccagaaatgt ttggcgcaga acaagtattg
    17041 atggcacgag cggaaaatga agaagttttt gactcaatcg ttgacttgat caatgacttc
    17101 aaacagtatt tcttgtctta cggggaacca gtttacgaaa acccatctcc tggtaataaa
    17161 gcaggtggga tcactacatt agaagataag tctttaggct gcacgcaaaa atcaggaagc
    17221 tctcctgtcg ttgatgtgct acaatatggc gaaaaaatca gaaaaccagg gcttagttta
    17281 ttgcaagctc ccgggaatga tcttgtagct gcctctgctc ttgcttcatc cgattgccag
    17341 ttggtcttgt ttaccacagg aagagggact ccgtttggct catatgtccc aacggtcaag
    17401 gtatctacga atacaactct ttttgaacgc aaaggtcatt ggatggattt taatgcaggc
    17461 gtcttattag aacaaccaat ggaagtaatc ttagaatcgt ttgttcaaaa aatcatcgct
    17521 gtagctagtg gagaagaaac gaaaaatgaa caaaatgacg ttcgagaaat tgccattttt
    17581 aaaaacggag taacactttg attttcataa aacagtataa aaagaaagaa ggaagattta
    17641 tgtttttatc tgatgatttt ttattaaaag acgattgggc gaaaaagctt taccattcct
    17701 acgcaaaaaa catgcccatt attgattatc actgtcactt agatccaaaa gaaatttacg
    17761 aaaacaacaa tttcagcaat ctgacagaag cttggttagg aggcgatcat tacaaatggc
    17821 gcttgatgcg agcttgcggc gtgcctgaag aaaaaatcac aggaaatgct tctgattttg
    17881 aaaaattttt agcttggtgt caaaccgtac caaaaattat cggaaatccg ctttacagtt
    17941 ggtcacatct tgaattgaag cgtttcttcg gtattgactt gttgataaat gaagaaaatg
    18001 cagagaaaat ttggacacaa gcaaatgaaa ttttggcagc tcctgatttt cgccgtcggg
    18061 aaatcgcaaa aaaatcgaat gtagaagtga tttgtacaac agatgatccc gcagacgact
    18121 tgcactatca tcagttgctt gctcatgaag aaccagattt gaaggttcta ccttcattcc
    18181 gtccagataa agcactcaat attgaaaaag aaggatttaa tgcatgggtc acactactgg
    18241 aagaagcagc tgaccaaacg atcaccacct atcaagaact gatagaagtc ttagggcagc
    18301 ggatagaata cttccatcaa catggatgtc gtgtttccga ccacgcttta gacatcttac
    18361 gctataaaga agcggatgaa acagtattag aagagatttt tcaaaaagca cgagctaaca
    18421 aactattatc ttcagatgaa atcgatgctt atcgtacaga aacactgata catttgaccc
    18481 acttctatca ttctcataat tggacgatgc agctgcacat ccatgcttat cgtaactgta
    18541 atacacaaat gttggaaaaa ttagggcccg ataccggcta tgatggaatg aatgatcttt
    18601 ctttgactat tcctctgcaa aaattattga accgtgcaga agaaacggat cagttgccaa
    18661 agacgatcct ttattcattg aatccgaatg actatcctgc gattttagct ttgatgggct
    18721 gtttccaaaa agaacagaat ggaaaactgc agttaggttc tggatggtgg tacaacgata
    18781 ctcgagctgg tatgcgcgat caattgacac aatttgctga tggtagtgct ttggggaatt
    18841 ttgtcggcat gctgacagat tcaagaagtt tcctgtccta cactagacat gaatatttcc
    18901 gtcgtgttct ttgtgaattt ttaggcgaaa tggtcatgcg aggagaagca cctgaagatg
    18961 aacaactttt aggaagtcta gttcaagcga ttagttatac aaatgctaaa aattactttg
    19021 gcttttttgc ctaattgatt aatagtaaca gtaacgaatc aaaaacagga tagtgaaata
    19081 acggaatccg tttatccacg ttttagagaa gaagacggac caaatttgct ccgtcttctt
    19141 ctcttgctgt tctgcttcat tgcctataaa cctttatgaa agaagagata acatgcctac
    19201 tatccatatt gccggtgatt ctactgcggc agaaaaagat gaaaacaagc gtcccgagac
    19261 aggttgggga gaaaaaatct ctgcttattt ttcacccgag atccagatca ataaccaagc
    19321 aaagaatgga cgcagcacca aatcatttat ccaggaagga aggttagccc gtctagcttc
    19381 acagtttcag ccgaatgatt tcttatttgt acaatttgga cataacgacc agaaaatcca
    19441 agaagaaaga ggtacgcttc ccttcggtag ctatttaaaa aatctgcaga tattttttga
    19501 atgcgcaaaa aaagcgaacg tccaaatggt cttactgaca tctgtgacgc gacgagatta
    19561 cttagaaaac ggaacattga atccagatat acttggagac tatccaaaag cgatgcgtgc
    19621 atttgctgag aaacaccata ttcctctttt agatgtgttc tcaagatcac aagaattgtt
    19681 ccagaccttc tcaaaagaag agacaaaaaa attctacctg catcttatac ctgatacttg
    19741 taaaaattac ccagaaggac taaaagacaa tacccacttt agtccagaag gcgctgataa
    19801 agttgcccga ataattgttg aattgataaa agaatcccgt ctgcctcttg ctaattattt
    19861 acaaaaagga gtttgagatg tttccattaa atttaggcat ccgtgcccac gatttagatg
    19921 ttcgaaatcg tgaagaaatc gttcaaaaag tatcacagct tcacttgtcc catatccaat
    19981 ttgcaccaca aaaagcattt cctgaacaac ttaaattaac tgacatgaca cttgggagtg
    20041 ctgcctattt tggtgaatac ttccaaaaaa acaatatcga actttccatt ttaggatgct
    20101 atatcaacct atccagcaaa aatcaagaag ttcgtgcaca agcattggca actttcaaac
    20161 agcaaattgc tctgtgctct gcctatcaag ccaagatgat tgctacggaa accggtagtg
    20221 tctctgtcgg ttacacaaaa gaaaatttta ctgaagaagc ttatcagatc gccagaaatt
    20281 ctatcattga attagtcgct catgcagaaa attttggcgt aactgttgct gttgaagcag
    20341 ggatcaatca tccaatctat aactatcagt tagctaaacg tttaatcgat gaagtagctt
    20401 caccaaattt aaaaatcatt ttagattgtg cgaatttgat gcataaagaa aatcatgctg
    20461 atcaggaaaa agtgatccga ggtgccttgg acaatttaca tggttatatt acagcgcttc
    20521 atctcaaaga ctatatcatg gaaaatggaa agatccgcat ggtacctgtt ggcaagggct
    20581 gtttagattt ccgcccggta ctgcattata tcaaatatga gcgtcctcta atgtacgcca
    20641 ctcttgaagc tactcctgag atttatgttc ctgaagcaat agaacacttg cagtttattt
    20701 accaagattg ttaaggagaa gattatggag gaacgaatac agaaaagtta tcaaaaatat
    20761 catgcaaatg cttttcagac attgctgcat ctttatcagg gaaattattg gcgttttgcc
    20821 atgtcaggac tcttttttat tatcaaacat cttccatctt ggttaatgcc gatcgctatt
    20881 gccaatgtaa tcaatgcagc ttcatcgaaa gattcttctc agttgcaggc catttatttc
    20941 aacgcttttt tgatggcttg tctgattggt caaaatatat tgacgaatta ttttcatgta
    21001 aaataccata gcacttcgat ccgtcaagta gaagctggac taagagcgac ccttatcaag
    21061 aaaatccaag aactatctat tccttatcaa aaagaattgc aatctggacg aatccagtca
    21121 aagatcatcc gtgatgtaga agctatccaa acgttgtcct ctcaagtatt tgtcagcgga
    21181 ttaaatatcg ctgtcaattt attcgtagct ttaggtatca cgctttataa aagtcctgtt
    21241 gttttcatgt ttttcttatt gactgttcct ctcgccgcat tcgttactct ctttttccgt
    21301 aaaaggatca gacagtccaa ctcagacttc aggcaagaaa tggaagagac ttctgcaaaa
    21361 gtgatcgaag tgatcgaatt agcaccgatt gctcgagctc atgggttaga aaaacacgaa
    21421 acaactcgct taaataagca ttttactcaa atctatgaaa aaggattcca cttagatatc
    21481 ctgcaatctg ttttcggatc agtcagctgg gctagttttc agatatttca gctgatttgt
    21541 ttgattttca ctgggatatt agctttacaa gggcgcatcc aacccggcga tgtcgtgatg
    21601 tatcagacgt atttcacgac gatcgtcaat tccgtttctg gaatgatcac gctgattcct
    21661 acgatttcaa aaggcatgga atctatcact tctattggcg aactgttgat tgctggcgat
    21721 gtagaaactt atcacggtaa gaaaaaaatc caacatttgg atggaaattt tcgttttgag
    21781 gatgtttctt ttctttatcc ggactcactc aaaccgatta tcgatcattt ttctctagag
    21841 gtgaaagctg gagaaacaat tgcttttgtt gggccatctg gctctggaaa atctacactc
    21901 cttaacttat tgattggatt tatccagccg acttcaggaa aaatttttct agatgatcag
    21961 cctttggatg aactggatat gcgaagtgtg cgaaattatt tagccgttgt tcctcaaacc
    22021 actttgttgt tctccgcttc gatcaaagag aacatcactt atggcttgaa aaacgtatca
    22081 aaagaacgtt tagatgaagt catcgaagct gcgcaacttt cttcactgat tgaccagctt
    22141 cctgatggtc tggatacctt agtaggcgaa catggcaaca agctgtctgg tggccaaaag
    22201 caacgtatct ccattgcacg agcgttgata cgtcagccaa aaatcatttt gctagatgaa
    22261 gcaacctcag ctttagataa ccagtcagag aaaaaaatcc agcaagccct gaattacctg
    22321 acagaaacac ccactacttt tattgtggct catcggcttt caacgatcaa agaggcagat
    22381 aaaatcgttg tgatcgatga aggaaaaatc gttgaaatag gtacttacga aaaactgtta
    22441 gctcaaaaag gctatttcta ccgcttgaaa aatgaatgac aaaaaatagg agctatgaca
    22501 agacttttgt cgtagctcct atttctgaat aaacggtgtt ctcctgccaa ctcctcggtc
    22561 acaagtcaca atagtcatca aacatgagaa gctgtttttt gaaattgttc cttgtaactc
    22621 agagttaaac ggtaggctca cgacctcttg gtaaatggtg ttcaagagac agcctatcgg
    22681 caataagaag caattttgtc atcgctcaag gccgaacgtc tctttcacac cctctgttgt
    22741 ttcataaaat agttttattc gctgtaaagg tcgcgaactg gtgtcatgat gatacggttc
    22801 aagtcattga tgattgtact gaagccttgc tctttttgca tcaagtcatt gattacttct
    22861 tctgattgga ttttcacagc catttcttgt gctttcattg catcttcgtc gctgaactct
    22921 tgtccactca tttgtttttc ttgtaattcg atttgcatcg cttggaagtc tttgaacagt
    22981 ttgtgtgctt cttcgtttgc ttgtactttc tcgtacgctt ctttcaacgc tttgaattct
    23041 ggtaattcac ggatctcacg ttcgatttga ttcgcgctat cgtagatgtt tgacatgtta
    23101 tgccctcttt tcttttagta gttactctct attgtaacat ggggttattc gttttcaaag
    23161 gggctactct ccttgatatt tccgccagag gtctttagcc gcttcaccaa ttccctgaac
    23221 gccttctttc accatatcgc cgaagttttt cagcccttct tttgcttgtt caccatattg
    23281 attcaagtta tcttgccaat ttccagactc tccattagaa tttcctgtgt ttccaacttg
    23341 atctgcagct accacttgtc ctcctgtggc ataggcatct gctacactaa atcctgtttt
    23401 tgaagtatac ggaaggatgc cttctgccgc tgatttgaag actttcccga caacatttcc
    23461 acttgtccct tccaaatagt gcagcttgct ggattcttcg taacccatcc atgtcgaaat
    23521 cactacgtct ggcgtgtaac cgatcatcca ttggtcattt gcttttgtgg catcgaaatt
    23581 agtttctgtg gttcctgttt tccctgctat cgtataacct gcaggattcg cagcagccgc
    23641 tgttccattt gaaaatgtac caagaagcat gctggtcatt tcatcggctg tttcctttga
    23701 tatgacctgt tttggttttt tgtttgtgtt atcgacgatc accgctccag tcgaatcaac
    23761 gattttcgtg ataagatgcg gcgtatacat ctgaccatca ttggcaaatg cactgtaagc
    23821 acccgccatg atcaatggag atactccttt ttccagtcca ccaagggcta agccataata
    23881 cttatcagat tttgctaaag gcaggccgaa ttcttctgct ttatcatatc ctttttgtaa
    23941 accgatctca tgcaaaagcc atacagtagg caaattcaaa ctttgtgcaa cagcttgata
    24001 catcggtact tctccttggt atgtcccgtc aaaattatgc gcatcgtaat aactttgcgg
    24061 ttcgtctttc aagatactgg aaggcttgta tccagcttcc agtgctggtg tatatacgct
    24121 taatggtttg atcgttgatc ctggtgaacg acgcatctgt gtagcaaagc tgaatcctct
    24181 aaagacatga tctcctcttc gtcctactag tgcttgtaca ccacctgtat tcggatctaa
    24241 agctactgaa gcagattgga ccatggcacc atctgaagca tttgctggga ataatgcact
    24301 gttttgataa gtagcttcta attgcttttg ataatcctga tctaaagctg tatagatttt
    24361 atagccttta ttcatgacat cttcttcatc aagaccatac tcgttaactg cttcatcgat
    24421 cactgcatca aaataataag gataacgata accagaatct gaattatcgt atgtgtcatt
    24481 aagatacgcg ctgatatctg tctggctttc tgcttgttct tgttcttttg tgatttttcc
    24541 gttttcttcc attacactta gtactgtgtt tttccgatca ttcgcatttt ttgggtgatc
    24601 gatcggatta taaataccag gacctttcag cattcctgcc aaagtagcag cctcagacag
    24661 cgtaacattc gttgcatcta ctccaaaata ctttctggaa gcgtcttgga caccccatac
    24721 cccgttgcca aaataggcat tgttaagata catggtcaag atttcttctt tgctgtactt
    24781 cttttcgatc tcgattgcca aaaataattc cttcgctttt cgatcaaacg tttggtctaa
    24841 tgtcagatac gcatttttgg caagctgctg agtgatcgta cttcctccgc cccctgatgt
    24901 tccgccagac aagaccattc ggactgctgc acgggcaatc cctttgatat caaacccgtg
    24961 atgctcgtaa aaattcctat cttctgtcga gattactgca tcttggatat agggagaaat
    25021 cgcgtttaat tctacatatg ttcctttttg ggcgtacagt gtgccagctt cttcgttttt
    25081 cttatcataa atgactgtcg attggcttaa tcctgatttt aacgtttcga catttgcttg
    25141 ttttgctaaa ataaagaggt agatacttgt cactaagaca cagatcaaac caatcagcaa
    25201 aatcaatttg ttcacatgat attttttcca gatgcgcttt cgccatcgat ggaattgtcc
    25261 gagatatggt ctgatccaac gccaaaaact gatcaaagca gcttttatct tttctaaaat
    25321 gctttgaaaa tccatttgct tcttctcctg tctatttgat tcaatcgtct tattttacca
    25381 tagtgaaaga gtgttttctt cctgcttaag ttgtagttca atatgttttt ttacgttagt
    25441 gcaagtagag taacataaga atgaacagaa aaatgtaagt tttttcttaa atatgtttta
    25501 aaaatggtac aattagtctg cgattgcact gaaaggagta cacttgtgaa aatcacgatc
    25561 actttaccta tgaaccatcc tgaaacaaca attcgcgact tgcttgaaaa ggaatggtta
    25621 gttcctagaa aggttcgaca ctttttgcgt atacgtaaaa acgtttggat caatcaacaa
    25681 cctgctttgt tccatcatga agtaaagggc aatgaccaga tcacattgtt attcgaagaa
    25741 acagattata attatcaaga agttcaatta ggagatgcga agcacatccg tgttttatat
    25801 gaagatgagc atttgattat agtggataag cctgcaggaa tgaagactca tccaaatgaa
    25861 ccgacagaaa acagtacctt actcaatcat ttagctgctt atctcgacac taaacagcaa
    25921 cgaccttacg ttgtccaccg cttggacaaa gaaacaagcg gtgccgtttt atttgcaaaa
    25981 aatccatttg tcttgccgat acttggaaga atgttggaac aaaaaatgat ctatcggaga
    26041 tatcaagcca tcgtatgggg agaaattgcc aaagatatga ccatcaccaa taaaattgga
    26101 cgagatcgcc atgatcggag aaaaagggtc gtagatgata aaaatggtca gagcgccttg
    26161 acacatgtca ctgtcaacaa agtactttct gaacaaacac aagtctattg tgttttggaa
    26221 accggacgta cacaccagat cagagttcat ttagctcata agggacatcc gatcgttgga
    26281 gaccctctct accaaaatag aacagctgaa cgactgatgc tccatgcatt agaactgcac
    26341 atgatccatc catttactca agaaaaaata gtcggtaaag ctttacctgg attatgggaa
    26401 tagaaataaa aaagaacaga gtcttatcag cagataatga taagactctg ttctttttgt
    26461 gtaatttgtc tgcttacttt tgatgttcgt gttctgctaa cagcaaacta ccgataatcc
    26521 cactttcatt cggcagtccc caacgaacga tatattcact tacttgaggt gtttccatat
    26581 aaccacccat caattcaaca aattgctgac ggattttttg aaggagatga tcctgattca
    26641 ttaccccacc gcctaaaata atcttttcag gtgctaacgt caacgtatag ttgatcagtg
    26701 cttgagcaat ataataagcc tgaatagccc aaacaggatg atcttgcggc agatcttgcc
    26761 ctttgatccc tgtgcgtgcc tcaatagacg gtcctgctgc taatccttct aaacaatctt
    26821 tatgataagg acaagtacct tcataatggt catctggatg gcgtttcacc caaatatgtc
    26881 ccatttcagg atgtgcgatc cctgaaaaaa tttcttcatt gatcacgaca cctgcaccga
    26941 ttcctgttcc cacggtaagg taaatacaac tattttttcc ttgtgctgct ccttgcttca
    27001 attccccata tgcagcagca ttgacatccg ttgtccaagc agcaggaata ttgtagcgtt
    27061 ccttgattgt gccaagaaaa tcgaattgtg cccacccttt tttgggagtt gccaaaacgt
    27121 atccgtaatt atcttttgtt ttgtcgatac caatcgggcc gaatgagcca atcccgattc
    27181 ccttcaattc ataacgatca aaaaattcaa atacctgatc caatgtttct tctggtgttg
    27241 tggtagggat acttaatttt tctaaaattt ctccaccatc tgaaaccgca cacacaaatt
    27301 ttgttccgcc tgcttctatt ccgccataca ttcgttttcc tcctcgaatc cgattgtccg
    27361 aatctcataa ggttggatta ttgggacaac cgcttgcttc gttggttctt caagcagatt
    27421 caatatgaca gtttttccat tgtctttagt tatttccaat ttttctaaat gactagacat
    27481 attgaaacct cttactacta ctttatcact aaacttgctg cgtttcaaag ctgtaattgc
    27541 aaacgtttca ctttttatcg ttaaatattg ttgttttgaa atcaatgttc cttcatgatg
    27601 tttgatttgt tgagtggaaa aaggaatctg tgctgcgtat gcatgcaaat aagtagagaa
    27661 tttttcttct ggtccatgta gttcaatgct gtaattgaac cgatgctcac ctagacactg
    27721 tgcttctggt gttggaaaat atccccaatc gcctagttct cccacactgc gaagtagcgt
    27781 cactgcgatt tgtccgtctt ctgttacttc atattcattc aaaccaaagt ttccgactgt
    27841 tacaccgtac tcttcatcat gaatattgac aaatgcttgc tgatgttgcg gattcgtggg
    27901 gttttcccaa cttttagaca catggtttgg gcgcttcacc acttcaaaaa tactatctgc
    27961 ttcgtgatgt tccacatgta actttgtagg aaacaagaca cgtaagcgat gatctttcat
    28021 ctgattgtcg agagtcgttt caaagtcgat ttttttacta tcttttctga ttgtcatgat
    28081 tgtttttatt tgaaggattc gtttttcttt ggatcgttct gcttttcgat aacgaaattc
    28141 cacaaccatc tgctgttctt tttctaaacg ctcgtctgca gaaacaggga tttccatttc
    28201 ctgtagaata cttatttttg ctatttccgg tgtatctact aatactttta cttcagagtt
    28261 ttctttgtta ctggatagga ttggctgatc acagaaaggt tgtttaaaga tatactcatt
    28321 tcctatatcg ccgatatctt caaaaatatg cagattattc aactttcgtt tgtttttgcg
    28381 atcttccatt gtaatggtac catttttttc aatcgtcaaa tgtagagggc cgttttcgat
    28441 gattcgtcca ctctgatgaa tcattgtttc cttttcttct gctttcgctt ccccttctac
    28501 aagcgcgaat gtttcccaag aaaatgctgg catgttttct aatggtaaag tcactttgat
    28561 cattcttgcc atatatggca cacggaaacg gtctttcggc aaatcataac caaattggac
    28621 agtttcttca gataaaatag ctggtatagt ggccccactt tttgtttcta catgatactt
    28681 acgtttaggc aaattttcta actcttgata tagttgttca ggtattcctt cagcgaatct
    28741 ttttctttct aattcgatgg ttatttctgc ttcaccagtt ttatccattc ctgcagtatt
    28801 tacaatgaca aatggaaagc tgtcttttgg gaatccggtc gtatcgattg ctgcggtcag
    28861 ctgttctaag gaatcttgtg caagatactt tccaacttca tctgcttttt caaaacgagg
    28921 tatcatttcc cgatggacag aatcgactga acaaccgcaa atactgtcgt gaggatgatt
    28981 ttgcattaat gtcttccaag cataatctaa ttgatcatgg ggatagtttc cactaacttc
    29041 ataagccatc gtcgcaagtg gttctgtaat attttctagc tgtcgctgga ccttggtatt
    29101 ccattgtttt aagtaaaccc gagcagatgc tgtattggct aaagtatacc agccatccgt
    29161 ttcttgactt gtcaattctc cttctactga gcctaagtct tctggcacat ctttttgaac
    29221 tgcttccaga tagtcagtaa aattagaatg aataaactcg taatctggaa acagttcatt
    29281 tgcgagatgg attgcttttg atatatcttt ttgtaccggt tgatgatcaa caccattcat
    29341 catcaataag tggttcgtag aggcatattg ttctgcatcc gctaattttt gcttccaaaa
    29401 ggcaagagct gcttcttttt ctactggaat ctcattcccg ttgctatacc aattggcaaa
    29461 tagcagacca aagattgctg tttgatcagg tcctttccac cacatctctg agtattggga
    29521 agaatagttt tctgcttcca aaacttgatt atcaaagcca attggtttta caccacggcc
    29581 aaacgctgct gctgaaatac cagcttgttt cattaattga ggcgtctgtc ccatatttcc
    29641 aaatgtatcg ggaaaatagc ctaacatgac aggggttccc catttacggc tttcttccat
    29701 gccaatcagc atatttctca cgttcgattc cgagctgata agaaaatcat cttgcaagat
    29761 ataaaatggt ccaattctca attttccttc attgattgct tgttggattg cctgtctttt
    29821 ttctggcctt acttgaagat agtcatctaa aataatcgtt tgcccatcca aatgaaaact
    29881 gttgaaggac ggatcaactt caaatagctc tagtagatcg tctatcaatt caatcagtcg
    29941 catgtgatgc tgttcgtaag ccatgtacca ttcacgatcc cagtggctat gagaaatgat
    30001 atatactttc tttttcattt acgatacctc ctacttttct actctaatat caaagtaatc
    30061 catgactaat tcgcaaaaca tcatatttgc ccaagaaaac cattctctcg tataattttc
    30121 aggattgttc acatcgaatc cttcgtgcat aaggtgtgtt cctgcgtcag ttgctacaag
    30181 taaattcaat atccgctctt tttcagattt gtctgacgtt gtcattcctt ccatagctaa
    30241 tgcaatcggc caaacgtatt tttctggcgt atgggaggat ccaattcctt ttgcgtactt
    30301 tccttcatag aaatatggat tttctttact caacaatgtt cttcttgttt gcagataatc
    30361 ttcctcttgt tcagaacaat atcccaagta aggtgcggcg atcagattgg gcacattact
    30421 gtcatccatg actgtggcgt ttcccattcc atctacttca tatgcataaa tcgtttgccc
    30481 tttttgatta accgttcgtc cgtgattctg aatcccctct tggatctctt tcttcaattt
    30541 ctgtgtttgc tgtacaattt ctttatcatc gagtatcgtt tgaaaaattt cttccacata
    30601 acctaacacg accaccgcaa acatattgga tggtaccaga tatccataac ggcaagtatc
    30661 atcgctagga cggaaaccag accatgtcat tcctgtaaaa gctgtttttg cacctcgccc
    30721 gtcattgatc agcgtatctt cttttcttgt cgtgtctcgt acaaatgtat aaggagagtg
    30781 ttcatgatct tgctctgttt tgaacacgtc caagattttt ttgatacctt caataaaaga
    30841 ttgattgaat tgatcagttt ttcctgtatt tttgtaaaga agataggcca actgtattgg
    30901 gtagcataaa gaatcaattt catattttct ttcccagatc caa
//
LOCUS       DACEXQ010000010        30588 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.010, whole genome shotgun sequence.
ACCESSION   DACEXQ010000010 DACEXQ010000000
VERSION     DACEXQ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.010"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            284..1369
                     /locus_tag="IXH04_000330"
     CDS             284..1369
                     /locus_tag="IXH04_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAQ1850965.1"
                     /translation="MSTENQSQKMIIKNWGYKATNVYIEKYYKFPSFLIQNSKYSKMK
                     DSSKIAYMLFKNEFRRALHEKWIDDDGLLYLEFTQADLMDKLNCYQGKVKTILNELET
                     YNLISVVKGEFNPKTKKNEKNKYYLLQPEISADDLFIQNSFDDDLSETIVKSTSSRNA
                     EIARREETAKTLDNTGNAEIARREKTAETLDNTGNAEIAQDYYINKSLDTKRHLKDTE
                     TDQLQNQVLLDNFVEIMKEDSINTFVPEKVLSLIKTFSNTYEEAQQTVTTIHKAKHKA
                     EELEKTVVVYEELESYGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFIEK
                     AIAAKLANEQRETFNELPTVSTENWLE"
     gene            2206..2538
                     /locus_tag="IXH04_000331"
     CDS             2206..2538
                     /locus_tag="IXH04_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAQ1850966.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            3129..3458
                     /locus_tag="IXH04_000332"
     CDS             3129..3458
                     /locus_tag="IXH04_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1850967.1"
                     /translation="MIQKFAKMSKTAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVDDKCPVKISFSDIATIIEGIYYGEVDEEISSTKDLLDFLDKNKDTFIVLTDYEE
                     VTTNEKL"
     gene            3445..3786
                     /locus_tag="IXH04_000333"
     CDS             3445..3786
                     /locus_tag="IXH04_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAQ1850968.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLTDDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            3900..4139
                     /locus_tag="IXH04_000334"
     CDS             3900..4139
                     /locus_tag="IXH04_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1850969.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKDVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            4206..4445
                     /locus_tag="IXH04_000335"
     CDS             4206..4445
                     /locus_tag="IXH04_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1850970.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKMMKQKHLKRLSVMQYINDMKIT
                     GKEKACLLGSMKNFEQLRRTYVKTGSNCQLLLEVS"
     gene            4572..5261
                     /locus_tag="IXH04_000336"
     CDS             4572..5261
                     /locus_tag="IXH04_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HAQ1850971.1"
                     /translation="MNKNNKTLFNPYVGVIIVFLLFSYFAYQGYQKDKIAEKVDGVTK
                     TIHIQEQAKTYQDNLTSLTDISKRQLITNQKQALEKDLKGEIAGKLVFSSTGFILPVF
                     QGANSDTLSLGVASTYYLDTEMGNGNYILAGYNVDTPSVLLSDMTDLSEGEEIDLIDA
                     HYKYQYEVSKRGKISDYSQVVQSKENNSFLSLPKAGKKPLLTLLAFQANNMKKCDVIQ
                     CELVNVEKINK"
     gene            5267..6433
                     /locus_tag="IXH04_000337"
     CDS             5267..6433
                     /locus_tag="IXH04_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAQ1850972.1"
                     /translation="MSHIQELVEKLINKMSAIFLLLLVSILYLIWTVGLNPPTILFIY
                     DNAAQLVTFFVGCLFLLNLYKVKRNDLFFIAAGILFYLFFSFFRSYRGDTFYGDLIVP
                     TIIAIILIVKWTKFTVIDRSLYLVVFYVFLLVTVYRVFTEIPVPEGQSIWNPSNKLSD
                     IWINTNTIGSSLMTLALLITGFASSFGKWYMRLLSLPALILAGATIWVCQSKAALYAL
                     VIFIILEILPKKVFRNTHLSFWMYAAVSVLALPISYFAAMSDKVHLFTGREEIWRKFY
                     ETLGEKTGQIFVGMKPFFFHRGNQILGNHNTYHALVNHYGLIGFVLSIILLLSFLWLL
                     VRKRELTNGQLTFLWAFLAIMCQSFMEETLTTISWVPIVFLMLAMATHQYKTTR"
     gene            6515..7186
                     /locus_tag="IXH04_000338"
     CDS             6515..7186
                     /locus_tag="IXH04_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAQ1850973.1"
                     /translation="MKKWLFGFLGVALIVVCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            7239..9215
                     /locus_tag="IXH04_000339"
     CDS             7239..9215
                     /locus_tag="IXH04_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ1850974.1"
                     /translation="MAINRYVKKAGMIVGMLGIIAGCFQGYRAYAEDVTQKTPPERVN
                     ITVHKLMYDQSTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PTEDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGELVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGEEHSFQIGDAVNYQGTIHIPTDIAGGTDGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGAISAVVYFKKKA"
     gene            9228..9980
                     /locus_tag="IXH04_000340"
     CDS             9228..9980
                     /locus_tag="IXH04_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1850975.1"
                     /translation="MIIRHPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTIPSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLEPNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            9996..10757
                     /locus_tag="IXH04_000341"
     CDS             9996..10757
                     /locus_tag="IXH04_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1850976.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGQKLEKQEGV
                     NGAKFSVYDVSDILQKMDVKDLTTDQIESQLKDRVKKLSSDQLKLISNGETKTIDQQT
                     GVFEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSE
                     ASQPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            10770..11030
                     /locus_tag="IXH04_000342"
     CDS             10770..11030
                     /locus_tag="IXH04_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1850977.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            11027..13117
                     /locus_tag="IXH04_000343"
     CDS             11027..13117
                     /locus_tag="IXH04_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ1850978.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLNNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTILAVSLLL
                     LVLATFGYAVLKFN"
     gene            complement(13153..15210)
                     /locus_tag="IXH04_000344"
     CDS             complement(13153..15210)
                     /locus_tag="IXH04_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAQ1850979.1"
                     /translation="MKLLNPKTVIAIGTFAATVALAGSADAASTNVMYRLYNPNNHEH
                     FYTSSTKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVTSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNTLDSAYKGLKKKPAPTVNKTELQALYNQVKSTAKGDFTEGSWNNFQTAL
                     SNAKKVLDDSKASQTQVNNAKNSLDSAFKGLQHKPKPSEYAVTVRHMNRQTGTLLKEE
                     HVSVKAGTSYTAKARTDLTNFEAPQYQVNGNETQTQTINTNTTFTFYYDEVFLVEVKA
                     RPNKVSDTDPNIQELYNYSKSYPVVYGQSITIEAPMFTNFVLDPRESSSNTVTLNNVT
                     SNQGINFAYTHQYNVTVNHINVDTNAVLSTETQTVYEGDSFSTSWKNMTDQNYFLCRN
                     DDSSVTVDKNGQRVINNVDQNRTITFKYKNISLSDLNNYVRQKELAWLNSYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENINSFYLYVD
                     EILSDTGAVNAMVMWKDSAAHNANLLYNNQSIVGLSHAYEVSADGSLSSDNVFISANP
                     IFK"
     gene            complement(15355..15546)
                     /locus_tag="IXH04_000345"
     CDS             complement(15355..15546)
                     /locus_tag="IXH04_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1850980.1"
                     /translation="MYADKEKIEWLLFETGKTAYEISKHSSVAESTIADLIKKKSAIE
                     KMRFDNAAKLTKYAESISS"
     gene            15734..16189
                     /locus_tag="IXH04_000346"
     CDS             15734..16189
                     /locus_tag="IXH04_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1850981.1"
                     /translation="MKKLIMVFTSLIAMIVILGGCGSEKNKQDSQDNGVLKPGTVWKE
                     EVGGLVHNLKIIDDTTWEYSESVWHPEQVQITVEKLKDYKGLERYKVIDSGGVDLFIG
                     KTALIVPYNKDGLETFKFIPTNEKEHRDKSEILKEYSTKSGYKLQKTSE"
     gene            16186..16428
                     /locus_tag="IXH04_000347"
     CDS             16186..16428
                     /locus_tag="IXH04_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1850982.1"
                     /translation="MIASLFIYPEGGIAVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            16446..16748
                     /locus_tag="IXH04_000348"
     CDS             16446..16748
                     /locus_tag="IXH04_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1850983.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQGAATSLKNNLMIFAAVAA
                     VASIVLGGIFFFFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGMFGQTPQGL"
     gene            16817..18871
                     /locus_tag="IXH04_000349"
     CDS             16817..18871
                     /locus_tag="IXH04_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1850984.1"
                     /translation="MYFTISGNTKKDKELIKKATKKVGKRTAFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVSQVSEVEVNDEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDLVEA
                     NEEPTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWERTKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCIE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMERHGQIQQELQAQIPKWKKEYEIQQQELKEQEEKQRQEEKEREQLELKKREQH
                     LAEQKVAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTAESSMENSKESDPSKAKEGSSDSTVHSNTSESSESNE
                     VK"
     gene            18871..21483
                     /locus_tag="IXH04_000350"
     CDS             18871..21483
                     /locus_tag="IXH04_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAQ1850985.1"
                     /translation="MRIRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLKTLKEIQQIYPQ
                     IPESKGSFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     RQSSMVVNDPKTELYVASKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPENEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHKGK
                     KFHEERVKVGYRGFCEINFDCHLEQGDHVCLKYNHQGKQYVAWYEFSQKVLKDEEGKI
                     VYRKKRGETHLPELEKEAVLTLDEKKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSFENETAKKLLIQFYEEQATDRLQEYVEKLIKNEEISEMI
                     RKRLLLEAEKAVKE"
     gene            21480..23858
                     /locus_tag="IXH04_000351"
     CDS             21480..23858
                     /locus_tag="IXH04_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1850986.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PDQAKKMSESSDISTVQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYRRY
                     ISSPGIILAGESSLSVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSAIE
                     DISQSFLLLAFTILELRIYTLMLSGIGDLHAVGKINGFLYVVALIVLTIALFKGSQSV
                     TKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGFGH
                     RKDITHDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGTVE
                     DLTEKAKDTLEEKLDGTRAKEFAEAVKNPSEALEKQKEKASAVKEGLKDAYGEGQVNA
                     AVKANTTSEKENQEGAAIPSAKDHQDELHSNENSIGEKDSEEGQHGQLPKTDPEQYRY
                     KSNINSENEKRISDLPKRNQEVQTDEDSIDLPNDNGIEVGTEKSTKIGLPKTQNLPMK
                     DSRLNQFKENDQSFVHQSPAGTVQMKSSRLPENGNLTTVSYNLAESGETENASVQNIV
                     TKEAAPKPINKVHNGYRPANVAFTAPTKINVTRETKNRNNELKEQLLKDIKP"
     gene            23919..24461
                     /locus_tag="IXH04_000352"
     CDS             23919..24461
                     /locus_tag="IXH04_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1850987.1"
                     /translation="MEDILIATFITALGAFLTIFVVTIKGFKDTDSLQKDHQSILDGQ
                     KTHTESIIVHLSEGFTYLKDAMPTFTVKLTEIFHSVEQISSELKEQRQQKDRQLGSLN
                     FRQETIQESLENVNRLNDEMQRVHLENEELRAQNYDLIQKKRNLEQKIVELKEENRQL
                     NYQLRRGKNRSRDLGNELEL"
     gene            24539..24871
                     /locus_tag="IXH04_000353"
     CDS             24539..24871
                     /locus_tag="IXH04_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1850988.1"
                     /translation="MDKYIHMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWLAIALVIVWRPYNNPGQRQIFAIIESIFFTDKNHYHALDPNRNY
                     HGTYKEDE"
     gene            24872..25465
                     /locus_tag="IXH04_000354"
     CDS             24872..25465
                     /locus_tag="IXH04_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1850989.1"
                     /translation="MRVKGQNSVDKKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            25487..27511
                     /locus_tag="IXH04_000355"
     CDS             25487..27511
                     /locus_tag="IXH04_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAQ1850990.1"
                     /translation="MLDVIKTAFSTQSNQKVIDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLAFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNAINPDFIETVNYVTNFQ
                     REMRKVEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEG
                     LVGKSMTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            27511..28710
                     /locus_tag="IXH04_000356"
     CDS             27511..28710
                     /locus_tag="IXH04_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ1850991.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWVFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYKLL
                     PSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKFPN
                     SKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSAYYKNCMSII
                     NTNKLMEYDEFAIKHWREGGGTGGTITGSWGNPFPGSSLDKNSFSGGQLFGKNPGGEF
                     RPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVYQE
                     FANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDPLP
                     FLNSSKK"
     gene            28726..29370
                     /locus_tag="IXH04_000357"
     CDS             28726..29370
                     /locus_tag="IXH04_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1850992.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTYKIEEVAK
                     NASDKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPVDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            29381..30187
                     /locus_tag="IXH04_000358"
     CDS             29381..30187
                     /locus_tag="IXH04_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1850993.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMILGFLILASPIYTHKKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLAHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIFYIH
                     EDKVKDHVKDEDYEQHAINYKVKGYKKEQKAAQKQIESFQATIDLNEELVKKLKDQLP
                     YQVAEDQENTENKINSYQQEIETSKTQMKDQEESIQKLQEKIDRMTKENI"
     gene            30210..30439
                     /locus_tag="IXH04_000359"
                     /pseudo
     CDS             30210..30439
                     /locus_tag="IXH04_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        10941 a         4728 c         6165 g         8754 t
ORIGIN      
        1 atactagata aatgaatatt aaagtataag ttatacatta aatatttaag gtataactta
       61 ataaacaacg tgtaaatttg cgattaaatc aacattataa aattattatt taaagtataa
      121 gttatacatt atttgtattt aaagtataac ttatacttat ttttttaata tataacttat
      181 accttaaata ctgaaagtgt tttattcacc cttaatacat ggtatagttc acttgttgaa
      241 aatcctgaat atatacaagt ttctaagaag aaggagaaat ttaatgtcta cagaaaatca
      301 atctcaaaaa atgataatta aaaattgggg atataaagca acgaatgttt atattgaaaa
      361 atattataaa tttcctagtt ttttaattca gaattctaaa tactctaaaa tgaaagacag
      421 ctcaaaaata gcctatatgc tttttaaaaa tgaatttcgt agagccttac atgaaaaatg
      481 gatcgacgat gatggtctat tatatttgga atttacacaa gcagacctaa tggacaaatt
      541 aaattgctac caaggcaaag taaaaaccat tttaaacgag ttagaaacat ataatttgat
      601 ttcagtcgta aaaggagaat ttaacccaaa aacgaaaaaa aatgaaaaaa ataaatacta
      661 tttacttcaa cctgaaataa gtgccgatga tctttttatt caaaattctt tcgatgacga
      721 tttaagcgaa actattgtta aatcaacctc ttctcggaat gcggaaatcg cacgtcgaga
      781 ggaaactgct aaaacccttg ataacactgg aaatgcggaa atcgcacgtc gagaaaaaac
      841 agctgaaacc cttgataaca ctggaaatgc ggaaatcgca caagattatt atattaataa
      901 atctttagac actaaaagac acttaaaaga cactgaaaca gaccaattac aaaatcaagt
      961 tttgctagat aacttcgttg aaatcatgaa agaagacagc ataaacacat ttgtaccaga
     1021 aaaggtatta agcttgataa aaacattttc taacacttac gaagaagccc aacaaaccgt
     1081 caccacaata cacaaagcaa aacataaagc cgaggaactt gagaagacgg tagttgtgta
     1141 tgaagaactt gaatcttacg gaattgatgc ggataaagga ttatatatga cgttgctgaa
     1201 agcttatcaa aaacaaaaga ctgagaaagt aaataatttg cagaatttga tttttgtgta
     1261 tgtaaaaaac tggtttattg aaaaagctat tgcggcaaag cttgcaaacg aacaaagaga
     1321 aacatttaac gaattaccaa cagttagtac ggaaaattgg ctagagtagt tgataaaaag
     1381 cgaacactat aaaaatgatc aattttaaat tagaactttt ttacaatttt aattttatta
     1441 caaatgttac aaataaatag ttattattcc ggagttttta gattaaaagt ttatgaaaac
     1501 aatcacaaac gtaggtttta atgtcataat cgttagatat tttagttatt tttcattcaa
     1561 aaaaacaggg aactagtgtt tgcatatgta tttttttgag ttataataat tcatgaagag
     1621 aggtaattca cttatttacc caagctgaaa agcctgatat tacttctttt caggcagtgc
     1681 tgctaatatt caattacact tttttgtata aatttagttg ttttaaattt aattgtctgc
     1741 tatgcttgtg atgaattgat tttcgttctg cacttttgga cgataaattt ggacaattga
     1801 aaatacaaaa aaaagaaagg ccctgtgaga ctcggaaggc taccaacctt cgtcgacgtc
     1861 cacaggtggc attaagtcta taccccttaa tgcgtttacc tttcagtgtt acggtattac
     1921 ccgtaacgga tttagtataa cagatattca aaagtgatac cacttttttt ggctgtgtta
     1981 aggttaaaaa tacgtgatat tacgatgaat ctatgcctaa gcataatgtt ataaaaatgt
     2041 ccgtggggct ctttgccgta tgacgaacgc tgaaattaga gggaacgcat gtttgaatgc
     2101 gttccctctt ttttgtttgt catgaacgaa aatcaattct ctatttttga aaaataacaa
     2161 gaaaaacttg ttaaaaatat ttaaagtaag tgggtggatt attttatgtc atatacagga
     2221 attttgagtc tcgaagatat ttgtcactac ggtaaacggt gtaccgctac agaaaagatc
     2281 acgaagaaac tatcaactgg tcaaagtaaa accgttgtgc agtgtaagaa atatataatc
     2341 cagaaagaca aagtctcaga agaaatgatt tattacattg ggaaacaaaa acaaataatt
     2401 ttaaaagatc ctatcccttt aaaagagcta tatccaacaa tcaaacatgt ttatgaccaa
     2461 aacggtgtac tgattggtcg aagaaaaaat ggagtcttgc gttgtaccgc aaaagggatg
     2521 gggcgtttaa tcagctgaat tgatcattct cataacttgt attttatcgt ttttcgcttg
     2581 cttgaaaata ttgtcgagac ttctaaagga acttagaatc actagaaaaa taaggagtag
     2641 acttgatcta tcaacaattc ccttcgggga gtgacggggt aggtttttac gattggtaac
     2701 gtaaaaaatt ggtcaatcga gtaaatgccc ttgtttggat agtggtgaaa tgaatttgtg
     2761 taaataatat gcacagagta aacaagaggg aatttagaag ttagacaagt tagaatagcg
     2821 cctagattaa tcatgagcag ctactaaggg atctaggctt tgacgcaaac tcatggaata
     2881 aggagtaggt cttctcgtaa tggggaggac gcacaagaaa ttgtgtcgtg tgctgcagta
     2941 catgtagata cttgcgagga cgttgatcat tggatcaatc gagcatttac cgctgtctac
     3001 tagggtttct cgtttcggac gccataaacg agtcgattta ttttatatat ttttgagtga
     3061 acgaaaaacg ataaagatat tcgttatgaa gtaaaactaa tttagtagat agcaaaggtg
     3121 gtagaggaat gattcaaaaa tttgcgaaaa tgagtaaaac tgcatggatt gaaaagcttg
     3181 aaaatgactt gagtgtcatg cgattagctc caaagaaatt caaacgagag attgcagaaa
     3241 aagaagcaat tttgtcggta ttgaatgcgg tagacgataa atgtccagta aaaattagtt
     3301 tttcagatat agcaacaatt atcgaaggta tttattatgg tgaagttgat gaagagatat
     3361 cttctacaaa agatttatta gattttttgg ataaaaacaa agataccttt atagtactaa
     3421 ctgattatga ggaggttaca acaaatgaaa aattatgatc cgaatatacg attgggcaca
     3481 catacgatta aggtttcttt ccaacgttgg gattacaaag gatttgtgac gtttagaaga
     3541 ggcggaaatt gtaaaggatt agatgtttta gcattagacg aggatgattt atacgatcaa
     3601 acattgactg ataatccaat tggattcggc ttgctacctg aagatgatga aggtaatgaa
     3661 tggtttaaaa tgacgcttac ggatgataat ggagacgaat tatctgttga agatacttgg
     3721 tcatatttga gtgattatat tgtcagcgtt gaaatcattg aatttgtagc ggataaagaa
     3781 gaataaaaac tggaggaata aattttacag ataaaggctt agaaggagtc acgacgaata
     3841 ataaaaaggt agtattacct taggcattga gtaaggaatt tatttaggag gaaaattaga
     3901 tggcgcgacc agttttagag gcgatggata tggatacttt gacaaaagga aggtatgtac
     3961 agctgttagc tcgtggttat tcacctaaag atgtattcaa atccctgtct aaaggaacag
     4021 atatggaaaa agaaagatta cgaaaagaat tcgattattg gagaacccat aatggaatca
     4081 aggatttgaa accagcacgg ccagtaatta aacgaaagaa aaagataacc caagactgaa
     4141 gtgtagagcg tatgtgatca taggcttttt tatttttgca aaaaacaaga aaggtaggaa
     4201 aactcatgaa ttatgaagaa atcgaaaaca gaaaaaaagt gtcgaaagaa atggaagaaa
     4261 agttgctaaa aatgatgaaa caaaaacacc ttaaaagatt atctgtcatg caatatatca
     4321 atgatatgaa gataacagga aaagagaaag cttgtttgtt aggttcaatg aaaaactttg
     4381 aacagttgag gcgaacttat gtaaaaacag gttccaattg ccaactatta ttagaagttt
     4441 cttgacgatt atgatatgaa aatgatcaat tatgatgtgg cattgttatt gaataaaaag
     4501 aatagtaaaa tttaacatgg tttatcttgg ttagatgtag gtaaattaat tcatatatag
     4561 aaggtagata gatgaataaa aacaataaaa ctttatttaa tccttacgtg ggcgtaatca
     4621 ttgtttttct tctttttagt tattttgctt atcaaggtta tcaaaaggac aaaatcgctg
     4681 aaaaagtaga tggtgtaaca aaaacaatcc atattcagga acaggctaaa acctatcaag
     4741 ataatttaac cagtttaacg gatatttcta agagacaact gattactaat caaaaacagg
     4801 cattagaaaa ggatctcaaa ggtgaaattg ctggaaaact tgttttttct tctacaggat
     4861 ttattctccc tgtttttcaa ggagcgaata gcgacacgct tagtttagga gtagcttcta
     4921 cttattatct agatactgaa atgggaaatg gaaattatat tcttgctggt tataacgtag
     4981 atacaccaag cgttttatta tccgatatga ctgatttatc tgaaggagaa gaaattgatc
     5041 taatcgatgc tcattacaag tatcagtatg aagtctccaa aagaggaaaa atttctgatt
     5101 acagccaggt agttcaatct aaggaaaaca attccttttt atccttgcca aaagcaggta
     5161 aaaaaccact actcacactg ttagcctttc aagcaaataa tatgaaaaag tgcgatgtga
     5221 tccagtgtga attagtcaat gtagaaaaaa tcaataaata agggaagtga gtcacattca
     5281 agaacttgta gaaaaattga tcaataaaat gagtgccatt tttctgttac tgttggtatc
     5341 cattctatat ttaatatgga cggtggggtt aaatcctcct acgatattat ttatttatga
     5401 taatgctgca cagttagtga cgtttttcgt aggctgtttg tttcttttga atctgtacaa
     5461 ggtaaagaga aacgatctat tcttcattgc agcaggcatt cttttttacc ttttcttttc
     5521 tttttttaga agctaccgag gagatacttt ctatggtgat ctgatcgttc cgacaattat
     5581 tgctattatt ttgattgtaa aatggacgaa atttactgta attgatcgta gcttgtatct
     5641 tgtagtattc tatgtctttt tactcgtaac ggtttatcga gtatttactg agataccagt
     5701 accagaagga caaagtattt ggaatccatc gaacaaatta tcagatattt ggatcaatac
     5761 aaatacgata ggtagttctt taatgacatt agcccttctt atcacaggat ttgctagttc
     5821 atttggaaaa tggtacatgc gattgctatc attaccagcg ttgattcttg ctggggcaac
     5881 gatttgggtt tgccaatcga aagcagcatt atatgcgctt gttatcttca ttatcttaga
     5941 aattttacct aagaaggttt ttcgaaatac acacttgtcc ttttggatgt atgcagcagt
     6001 ttcggttcta gctttaccga tttcttattt tgctgcaatg tcagacaaag ttcacttatt
     6061 tacaggacga gaagaaattt ggcgcaaatt ctatgaaaca ttaggcgaaa aaacaggaca
     6121 aatatttgta gggatgaaac ctttcttttt ccatagagga aatcagattc taggtaatca
     6181 taatacgtat cacgcactcg tcaatcacta tggattgatt ggttttgttc tatcgattat
     6241 tcttttgctt agtttcttat ggttgctcgt aagaaaacgt gagctgacaa atggacaact
     6301 aacattcttg tgggcttttt tagcgatcat gtgtcaatcg ttcatggaag aaacattaac
     6361 gacaatcagt tgggtgccaa ttgtattctt aatgttagca atggctacac accaatataa
     6421 aaccaccaga taaatgaaat agaatagtaa taagagaaaa cactttttcg ttgtgaaaaa
     6481 gtgttttttg ttttgtctga aaggaggcag atggatgaaa aaatggttat ttggtttttt
     6541 aggagtcgct ttaatcgtcg tttgcagtgt gtttggctat gttagctacc aaaaacatga
     6601 aggagaggtg tttaagcaaa atatagaaaa aaagatgccc gtagatcaaa tcaatgcaca
     6661 cgcaaagagt tataaggagg atgcaacgaa cgtcaataat gatatgtctt tggggcaaat
     6721 gttgtccata caaaaagaag caatcgaaat gggcgtcaat aaacaagtat ttgcacaaat
     6781 tcagattcct gcacttggtt tagcattgcc aatttttaaa ggggcaaatc aatacacttt
     6841 gagtcttgga gcggctactt atttttatga agatgccgaa atgggtaaag gaaactatgt
     6901 cctagcaggt cataacatgg aaatgccagg tgttttattt tctgatattc aaaagttgtc
     6961 attaggggag gtgatggact tagttagtaa cgatggcgtc tatcgttaca aggtaacgcg
     7021 taaatttatc gtacctgagt actttaaatt aatcgatggc gttcctgaag agaactcttt
     7081 cttgtcttta ccaaagaaag gagagaaacc cttattaacg ttgtttactt gtgtttatac
     7141 gtcacaagga aaagaaagat acgtggtaca gggagagcta caataacaag aatttaatca
     7201 acgcgagtcg ttgaaatatg aaatgaggag agtaaaaaat ggcaataaat cggtatgtaa
     7261 aaaaagctgg aatgattgtt ggaatgttag gaatcattgc aggctgcttt caggggtatc
     7321 gagcttatgc agaagatgtc acgcaaaaaa cgccaccaga aagggtcaat atcacggttc
     7381 ataaattaat gtacgatcaa agtacgcaat tgaacgttga tattgacgga atcaaaaatg
     7441 atggttatac gcacgatcaa tatcctacag gtgtaacgaa atacaacaag gcagattatg
     7501 gtgatgttga gtttacgtta ggaaatatta cggatcaagt attaccgact gaagattctg
     7561 atctaaccaa cgcgaaagtg gacgaaatcg ttaaagatgt agaagacaag ggatctaaca
     7621 gtgagtacgt taagaatgca accaataaaa ttacttcagc tgtagatgaa aatggtgaga
     7681 tcacttttgc tgatcaacca gcctatgtca attccaaagg gaatgtatat gtggtttatg
     7741 aaagtaaatc cgctgctgga ttagtcacac aaaaagcaaa accaatggtt gtgattgcgc
     7801 caatgacaga caacacagga tcaggatttt taaaagatat tcatttgtat ccaaagaaca
     7861 ttgtttctaa gttgagtttt gaattaacga aatttggtga cgatggcaca gctcaaagca
     7921 aacaaacacc tttaaaaggg gcaaaatttg agttatacaa gggcgaacct ggaaaaggaa
     7981 caaaattagg agagcttgta tcggatgatc aaggaaagct gactgcaact gatttgacgt
     8041 taggaaaata ctactttgtg gaagttcctt cagaagttgt agttggatca gatcaagaac
     8101 caacagcaga ccaatatttg ttaggcgcag acgctcgcaa cgatgcgcac aataagttaa
     8161 cctttgaaat tacgaatgac ggcgtgacaa gtgatttaaa agcttcctac gtgaactata
     8221 aagcaccagt gatcgataaa acagtgacaa atggaacagg agaagaacat tctttccaaa
     8281 ttggtgacgc agtgaattat caaggtacaa ttcatatccc tacagatatc gcaggtggaa
     8341 cagatgggat tacagtaaat ggtgtaaaaa gtgaaacttc tccttacagt gtatttaaat
     8401 ggggcgacac agcaggtaaa ggactttctt atgttgctga aaaagcagat attaaagtaa
     8461 caaataatga tggttcagtc gtattaaaag aaaatactga ctataaaatc gaaaattctg
     8521 atagtggttt tgtgatcgat tttatcgtaa ataatggaca agtaagtgat acagttgcga
     8581 atttacatgg tcaagatcta aaaatgactt ataacatgtt tgtgaatgat agtgctgaaa
     8641 tcgcaaatcc attaacaaat agcgtggatt tcgtttataa caataatcca tttaatcagg
     8701 aagaacacca cgaaaaaacg aaagctgacg ttgtgactta tggcgcaaaa ttcttaaaag
     8761 tcgatagcgg cctatttgga acaggtatca aagcaacacc gcttgaaggc gcagaatttg
     8821 cagctaagaa tgcagaaggt aagtactacg gtggacttac tgatacagat aaagatggtg
     8881 tcaaagaagc agtttgggta gacgatgtag caaatgcagc aatcctaaaa tcagataaag
     8941 agggtcattt tgaaatcact ggtttaactg aaggagatta ctcactggaa gaaacaaaag
     9001 ctcctgaaaa ctatcaaaaa ttaacaaaag agatctcttt caaagtagac aaagattcat
     9061 acaaagaaga aaaccgaatc acgatcaaaa ataatcaaaa agcttccgta ccaatgactg
     9121 gtagcaacgg tttccaatct tacgtattga tctcttgctt gttgcttggg gcaggcgcaa
     9181 tttctgcagt tgtttacttt aagaagaaag cctagaagga tcagctaatg attatcagac
     9241 accctaagaa aaagcgaatc atgggtaaat ggatcattgc tttctggtta ctttcagctg
     9301 ttggtgtctt acttttgatg cctgctgaag cgagcgtggc gaaataccaa caaaatcagc
     9361 agattgccgc aatcgatcga acaggaacgg cagcagaaac ggattcttct ttggatgttg
     9421 ctaagattga gttaggcgat cctgttggta ttcttacaat tccaagtatt tctttaaaac
     9481 taccaattta tgacggaact tccgacaaaa ttttagagaa tggtgtaggg attacggaag
     9541 gaactggcga tattacagga ggaaacggta aaaatccttt aatcgcaggg cacagtggac
     9601 tttataaaga taatcttttc gatgatttgc cttcagttaa aaagggagaa aagttttata
     9661 tcaaagttga tggtgagcag cacgcttatc aaatcgatcg gattgaggaa gtgcaaaaag
     9721 acgaactaca aagaaacttt gtcacttact tagaaccaaa tcccaatgaa gatcgagtaa
     9781 cgctaatgac atgtacgcct aaggggatca acacacaccg tttcctagtg tatgggaagc
     9841 gagtaacctt tacaaaatca gaattaaaag atgaagaaaa taagaaacaa aaattatctt
     9901 ggaaatggct tttaggaagt actgtttttc tttcagtcat gatcattggt tcgctatttg
     9961 tttataaaaa gaaaaaataa aggagggtaa ccaacatgag aaatagaaaa tggctatttg
    10021 tttgtgcagc cgctcttttt tctttcactg cctacacaca gttgacgttt gcagacagac
    10081 aagaaagcac ggatagcaca gaacaaacaa tccaaacgtc agatagcagc acgattcaaa
    10141 gcagcgatga aaccgtcgat tcgtcaacag ttgattcttc agaagaacct aaaattgctg
    10201 aagacgatca aatagtcaaa cagaccattc acgttcaaaa aattatttct tccgaacaat
    10261 tagacaacca ggggcaaaag cttgaaaaac aagaaggagt aaatggcgcg aagttttctg
    10321 tttatgatgt atcggacatt ttacaaaaga tggatgttaa ggatctcact actgaccaaa
    10381 ttgaatccca attaaaagat cgagtgaaaa aactatccag tgatcaactg aagctaattt
    10441 ctaatggaga aacaaagaca attgatcaac aaacaggtgt ctttgaattc tcggtagaag
    10501 tacaggcaaa tcagaaacaa gcttattata ttgtcaatga atcaagtcct gaaaacattt
    10561 ctaatagcga ggatattttg ctattaacac ctgtttctga taaaaatggt gaattcttaa
    10621 aggatgtgtg gatttatcct aaatcagaag cttcgcaacc taaagaagaa gtaaaaaaaa
    10681 tcgtatctac tggcgtaaag aaaaacttct ttgagaattg ttgggatttt gttactcact
    10741 tgttttctag agattaagga agaaataaaa tgaaactgat caaattatta ttattcaatt
    10801 tttcttgttt aaccgcagtc tttttgtact gcggtttatt attttctaaa ctatctattt
    10861 tgccaagcta ccaactatac ctaatgagga ttttaaacat accagctgct tccatgccgc
    10921 tagatattat ggaaaagctc attaggaatc cattatttac tacgatcaat ttgatcgtta
    10981 ttgtcggaat ttcactagtc atttataaaa aagaggggat gaagggatga attttaaaca
    11041 ctcgtggaca agacggttaa aatatgtttt tcccataatg atgatgttag gtacattgtt
    11101 tgggttaaaa acagccaatg tctttgctga aaaagttatt gtcgatccgt caaatccaat
    11161 ccagaacgta aagaataatt ttattattcc aaaagatgtt cctaatgcaa aaacaaatat
    11221 tactgtaaac ggagcgtcaa aagtttacaa atatcatgtt gatgccaaca aaggtggttt
    11281 tacagacgac tacgtaggat tagtggaggg aaacaaaaat atacgctatc cttctttcca
    11341 aaaagaatac ggtgaaacaa accttgtttt agaaggcgta acaaccaaca ccaaagtcaa
    11401 tattgactat ggaaaaatcg gtacgtataa cgggaaaaaa gtcaatatca agctagttct
    11461 atctaatatt cacttatact cggatacgtt gccgtggaat ctactagata acaactatac
    11521 aaaaactcat tttcgagacg atggctataa aaatacgaat ggtgcaatga gtaaatctaa
    11581 aaaaagaaca gtactttgga tctcagacaa tcttttctca ggaattgttt accattcaac
    11641 acaaatgaat gtgcagctag tagccacata tgaggacggt agccccgttc aattttcagg
    11701 agatacattt atcagtttta actctttgaa ccctgcagga ggaaaaagca cggatcttaa
    11761 aggagaatat gcccactatg acaaaatgaa tacgacagat tggtatgtca gaaaagatac
    11821 ggttctttca gaatttaaat ctttctacaa caatctgaac gtggtaggtg gccacccagg
    11881 aggaagctct aagctgacac aagcggataa tgatttcaat aatttgcatg ataaattagg
    11941 agatccgaag tttggtcaag gtactgtgtc ctttaaaata tctgaagcaa atccgacgtt
    12001 tgtgattggt tcttctaatg ttcaaacctg gtttacttta tcttctgcga caatctttag
    12061 tgttgttcct gatcaacctg aaaaaacagg ggttgataaa aatggaaata acgtgaacga
    12121 taaaatgttg caagtaggag acacgattca atatcgaatc aaacagaagg tgaatcgatt
    12181 aggagtggat ttactagcgg tttacgatcg atttgagtta atcgataatt taccaaaaga
    12241 agtaaattac gtcgatgcac acgttgaaag tggtacaaat aaaaaattcg atgtatctgg
    12301 tgaagtgacc tatgacaaaa caaaacacca ggtaaaatat gcagcgaaag ctgatacatt
    12361 aaaaaatcga atgaagtata acggcgaaac ttacgaatta gtgatcaatg taaaagtcaa
    12421 tgaattggcc aatcaaaata gtgtagccaa aaatcaggga acgtcgatca ttaataaagt
    12481 agaaaaggat acaaatatca ttaccgttta tttccctaaa attccagtga aagaagtaca
    12541 acaaaatgga aaagatgtaa acggacgaaa tgacggtaag aagggtacgc cgacagctcc
    12601 tttaaacgca ggatctgaag tacaatatct agtcacacaa aaatggcaca caaaaggcgt
    12661 agacgctgcg tcagatcact acaaacaatt tagtattcaa gatccaattg aagctcgttt
    12721 gacatataaa gaaggttctg cgcaagtaat agataagagt aaaggaaaag atattacttc
    12781 tgaaggcaca ctaacctatg atagcaattc aagaacgctg aagtgggaag cttccgctga
    12841 cttcttaaat aacaatcttt tagatggacg agaaattcag ctgatattta cagctaaaac
    12901 tccattacaa tcagaaaaaa atattgataa ccaagccgta gtagcagtag ataatgtttc
    12961 taataaaaca aatgttgtga caattggggt tgatcctaac ttgccacaag ttattgttcc
    13021 taaaacaggt tctacacatt tagtaacgat tttagcggtc agcttactat tacttgtgtt
    13081 agcaactttt ggctacgcag ttctgaagtt caattaaaca aaaaaacaag gctgaaaatc
    13141 agccttgttt ttctatttga aaatgggatt ggcactaata aatacgttgt ctgaagaaag
    13201 tgatccgtct gcagaaactt cataagcatg ggataaacca acgatacttt gattgttgta
    13261 aagcaagttt gcgttatgag cagcgctatc tttccacata accatagcat ttactgctcc
    13321 tgtatctgaa aggatctcgt caacgtaaag gtagaaacta ttgatatttt ctcctttagc
    13381 accgtagcag cctaagcttt ctaagagatc ttggaaagta ccgccgccag gtcggtaatg
    13441 actgaagctt acttgtaatt cttttgcacg aatatctgca gcttgttgaa cgatatcatt
    13501 gaattgcatt ggggcaacgc cattttgttg acggtagcta tttaaccaag caagttcttt
    13561 ttgacgcaca tagttgttta aatcacttag tgagatattt ttgtatttga aagtgatcgt
    13621 tcgattttgg tcaacattat tgattacacg ttgtccattt ttatcaacgg taacagatga
    13681 atcgtcattt ctacacaaga agtagttttg atcggtcata tttttccaag aagtagagaa
    13741 actatctccc tcgtaaaccg tttgtgtttc agtagataaa acagcgtttg tgtcaacgtt
    13801 tatatggttc acagtgacgt tgtattgatg agtataggca aaattgattc cttgattact
    13861 tgtaacgttg tttaaagtca cagtgttgct agagctttct ctaggatcaa gaacaaaatt
    13921 agtgaacata ggtgcttcaa ttgtgatgct ttgtccataa acgacgggat aactcttact
    13981 gtaattatag agttcttgga tatttggatc agtatccgag actttatttg gacgagcttt
    14041 cacttcaact agaaagactt catcatagta aaaagtaaat gtggtatttg tgttaatcgt
    14101 ttgtgtttgt gtttcattcc catttacttg atactgtggt gcttcaaagt tagtcaagtc
    14161 ggttctagct tttgcagtgt aacttgtacc agcttttact gagacatgtt cttcttttag
    14221 cagcgttcca gtttgtcgat tcatatgacg gacagttacc gcatattctg aaggtttagg
    14281 tttgtgttgt aaccctttaa atgcagaatc aagactattt ttagcattat ttacttgggt
    14341 ttgagaagct ttagaatcgt ctagtacttt tttagcattg gataaagcag tctgaaaatt
    14401 attccaagat ccttctgtaa aatctccttt agcagttgat tttacttggt tgtacaaggc
    14461 ttgtaattcc gttttgttca cagtaggagc tggttttttc tttaatccct tgtaagcaga
    14521 atctaatgtg tttttggcac tatccacttg tttttgagtc gcttttgagt catttaacac
    14581 ttttttagca ttgtttaaag cggtttggaa tgcgttccag gtattgtcgg tatagtcacc
    14641 ttttttagtt cccttaacgc tcgtataaag agcttctaaa gctgatttat tcactttggg
    14701 tttagaggtg tcagcaccat accaaccaat accttcgtat ttccagccat agtctttggt
    14761 taaccattgt acttctttcc aacttgcagt gtagtgatgc gaaccagagg taacacctgg
    14821 aaggaaaagg cggtagatcg gtttattatt tttttcggca gaacgccagg caggcccttc
    14881 ataagtccaa ccgtaatttt tggtcaacca tttcacttca tcccaactag ctgtgtaatg
    14941 gtgatcacga aggcctttat tgtataaacg atagacaagg tggccaccgc tagtcggtgc
    15001 ttcccaagcg gggccttcat aattacccca cttaattttc actaggtgat cacgttcttt
    15061 tgtgctagag gtgtaaaaat gttcatggtt atttggatta taaaggcgat acatgacgtt
    15121 agttgaagca gcgtcagcag atccagcaag agcaactgtg gcagcaaatg ttccaatagc
    15181 tataacagtt tttgggttta ataatttcat gttttcaact ccctattaaa tgattaaata
    15241 ttttgtttac attcataata taccacggta taccgaggta gtcaacggta taccgtggaa
    15301 attttgaaat aaaaaagccg ccctatgaag agtgtaagga tggctgttta tatgctatga
    15361 agaaatggat tcggcgtatt tggttagttt ggcagcatta tcaaatcgca ttttttcaat
    15421 cgcacttttt ttctttatta aatccgcaat tgttgattct gcgactgaag aatgtttgct
    15481 aatttcatat gctgtcttgc cagtttcaaa tagtaaccat tcgattttct ctttatcagc
    15541 gtacattgca atgctccctc ttgatttttt ctttattata gctctttttt gtaataaatc
    15601 acaatatcaa gaggaaaagt ttctatagaa aggtgggaaa ttatgagtat ccgttccccg
    15661 tgtatcaaaa tgaggtttgt tgctactttc gttatttaat atactaaaat aaaacaacga
    15721 aagaaggaat agaatgaaaa agttaataat ggtatttact tcgttgattg ccatgattgt
    15781 tattttaggt ggatgtggta gtgaaaaaaa taagcaagat agtcaagata atggcgtgtt
    15841 aaaaccagga acagtgtgga aagaagaagt aggcggactt gttcataatt taaaaattat
    15901 agatgataca acatgggaat attccgaaag tgtttggcat cctgaacaag tacaaataac
    15961 agttgagaag ctaaaagact ataaaggatt agaaagatat aaggtaatcg attcaggtgg
    16021 agtagattta ttcataggta aaacagcctt aatagttccc tataacaaag acggtttaga
    16081 aacgtttaaa tttattccaa caaacgaaaa agaacatcga gataaaagtg aaatattaaa
    16141 agaatacagt actaaatctg gatacaagtt acaaaagact agcgagtgat tgctagtctt
    16201 tttatttatc ctgaaggagg aatcgcagtg gcaaaagcta aaacgccatt tgaaaaagcc
    16261 gcgtctaaaa tcttagaacg tcgcggtgta tctgaaacac agtggaaaca agaagtcctt
    16321 gaaaaagcac aatttgacct attacgggga aaagacaagg acttagaagg atatatctat
    16381 caacgtgaac gtgaaaagct tgttttcgat gaaattatta aacactaaaa ggagcgaaca
    16441 attttatgtt tgaatcaatt acaatgaatt ttttaacaca accgaaagta gtagctgcgg
    16501 ttgatatttt tagagcatta caaggtgcag caactagttt aaaaaacaat ctgatgattt
    16561 ttgcggctgt agctgccgtt gcctccattg ttcttggggg gattttcttc ttctttggcc
    16621 gaagaggagc tgaaaacggg aagatgcacg taggttatgt attgatgggg gtaggagtta
    16681 tattagctgc cgcctctatt gtggtttcgt tatttggaat gtttggacaa accccacagg
    16741 gactataaaa aataaataga aggagtaaaa agtacgtggc tactttttac tcctttttta
    16801 tgaaaggaga atactcatgt actttacgat ttcaggcaat accaaaaaag ataaagagtt
    16861 aataaaaaaa gcaacgaaga aagtgggaaa gcgtactgcc tttttaccga aaacaattaa
    16921 atattctgtc attgaaacgg aagaacagct acatgtgtta ggacaagtgt tagaacaaga
    16981 gaatcaatct tctgttaaat tgactctcac gttacatgat aaagaaggaa aaatagaaaa
    17041 ggctggcaca gaatacgttt ttggcccact ggattatcaa atttcagaag gtttttatcc
    17101 tacattgatg gatttgatcc gccttgatgt gtttaaggaa acggatgaaa ccatgagtgt
    17161 ggaagagtct tatcgaaacg aaaaactgct acaagtttta gaagaagagt tagagagcga
    17221 ccaaagaaaa gtgagtcaag tgagtgaagt cgaagtaaat gatgaagtac catgggaacg
    17281 accaatcttt ccaaagaagg agtcaaaaga aaaagagaca gtctatgttg acgaacaagc
    17341 tgatattccg actcagaaat cgatatttga ggaagagaac gatgtaatgg gtcaattgga
    17401 ggtagatccc tttgaagaaa caatggagga tgaagagacg gccgaagtag aagttgatct
    17461 agttgaagct aatgaagagc caacatggac tgaaggggat tctcaggaat atattgaacc
    17521 acttatcagt gaagaagtag agaaaaacga aacagagata caaaaggcaa ctgtatttga
    17581 ttatattttg gatcaatatc cgccagagca tgaatggatg aaagaaaaaa ataaattatt
    17641 tttaaatgag ctttattcga aatatcaatt accccaaacc tatcaagcat ttttagagag
    17701 taaagaagaa ttaatctcac agggtaaagg cacactttta gaacagttag agtctgttca
    17761 atcaacaaat tggcatgagc ttgcacaaga agatttatta gccacttttg aagaacgtca
    17821 acaagaaaca gatcaagaaa taaatgagta tgcgacggaa cagtcatcta attgggagag
    17881 aacaaaagag gaactaaata aagaagaaca acgagtgatt gaagaagaag tacaaaaaat
    17941 taaagagcga tacgaaaaga aacgcgacaa tgcgtattcc ttgtgtatag agaaaattaa
    18001 taactttact aaaactaatc aagaagcttt atcgaaagaa aaagaaaact tgttaaaaga
    18061 acgtataaaa gaacgaaaag aagagttcta ccgaggttta cgtacagata agctaaagat
    18121 tagcaaagaa ttaaatgaac aactgaataa actttatcaa acaacgactg aagttcttat
    18181 ggaaagacac gggcagatcc agcaagaatt acaagcacaa attccaaaat ggaaaaaaga
    18241 gtatgaaatt caacaacaag aattgaaaga acaagaagaa aaacaacgac aagaagaaaa
    18301 agagcgcgaa caattagaac taaaaaaacg tgagcagcat cttgcagaac aaaaagtggc
    18361 gatagaaaaa caacggttag agcaagaaaa ggaaaaagaa gcagctgctc gtaaggaacg
    18421 tcaagaacaa atggatacat tgcgacaagc acagttgaat tatctagcac aacctcaacc
    18481 agtagctgct gttcaaacgc cagtgaacaa ccaagcaaat ttaactcctt caccacagcg
    18541 ttcctttaac ggatggatgg tgggatgtat tgcaagttta agcttgcttc taggtggagg
    18601 aacagtattt gcttttaatc atatcaatca atcttcacag gcagattctg tagccacaac
    18661 ggcgtcaatc aaggaagaag cccaggaaca agcaagaaaa gagtatgaac aacaattgga
    18721 agaattattg aatcgtacca atcagtcaac agcagagtca agtatggaaa atagcaaaga
    18781 gagtgatcct agtaaggcta aagaaggatc aagcgattca actgtacact caaacacttc
    18841 cgaaagttca gaatcaaatg aggtgaaata atgcgtatta gagaaacaac gagacgaaaa
    18901 gacaaacgat tcaatttaga aaagaatagg tatctttctc atttctatcg ctatactcct
    18961 tcaggagaag agctaccaga aaaacatgac aaacgtattt ctcaaaagat tttttatagt
    19021 ggcttagctt atcttccttt aaactatgtt gttacaagaa taccagaagc atttcatcga
    19081 tttgagatta ttccaggaag tttacaagga gtgaatattt ttcagttaca agaaatgccg
    19141 cagttattcc attttgattc aactggtttt tggaatcctt tgaccccaac aacaagttta
    19201 ggcacacgtt tagttgtttc tacgatttgt gttgttccag ctgtacttat gggattgaaa
    19261 atggacatgt ggtttcgtcc gctagcagat gggcaaaaag gagataaccg attaaaaacg
    19321 ttaaaggaaa tccaacaaat ttatccgcaa ataccagaat caaagggaag ttttcctggt
    19381 tatggcggaa taccaattac tcattataaa aaatactggt atattcagac ggatacagta
    19441 aatacttgta ttgttggtac ttcccgctca gggaaaggcc agatagaagt tttagcaacc
    19501 attgacaatt tgtcgcgtgc agaaagacaa agttcaatgg ttgttaatga tccaaaaaca
    19561 gaattatatg ttgctagtaa agatgaacta gaagctagag ggtatgatgt gtatgccttt
    19621 aatatcaccg atccactgca atcaatgagt gataatccat tggcactcat tgtgaaatac
    19681 tggaagcgag gagacataga caccgcaaca cagttaacca atacttttac gaatacgatt
    19741 tattatgatg caaatgcttc agacaataaa tactttaacg aaaatgcgca gaaagctgtc
    19801 aatgcgctta tttttgcgtt attggaacaa gcggatcaaa caggggatta ctccaaatta
    19861 acaccaaata atctcgttga actactttca gaaattggcg gttttaatta ttatccagat
    19921 cctgagaatg aattcaagca gctcaatgcc ttagatgagt tcatgaatca gttaccccct
    19981 ggaaatatag caaaaaaaca atatggggca acaaaaattg gatctgataa agcgaaaggg
    20041 aatatcttgt ctacggcaat tacaggattg aatccattta ccctaacgaa aattgcgaag
    20101 atgacttctc aatcgaccat tgattacaag tccattggtt ttccgaagta tttggatctc
    20161 aaattacatg aaagcctatt aaatcaacgc attgtcgtgg aattttatca taagggaaag
    20221 aaatttcacg aggaacgagt caaagttggc tatcgtggtt tttgtgaaat caactttgat
    20281 tgtcatttag agcagggaga tcatgtttgc ttaaaataca atcatcaagg aaaacaatat
    20341 gttgcttggt atgagttttc tcaaaaagta ttgaaagacg aagagggaaa aatcgtttat
    20401 cgaaaaaaac gaggagaaac tcatttgcca gaactagaaa aagaagccgt tctaacatta
    20461 gatgagaaga aaagtaacct attagtagag tctattcgga tgcactatac cgataaacca
    20521 atggctattt ttatgttaac gcctgattat gatccatcta accatatcat tgttagcatc
    20581 tttcttagtc agttgtataa agaattatct acgcaatgtg ttaagactaa aggagataaa
    20641 tgtcaccgta ggattcattt tattttagat gaatttggaa atatgccacc gatggacaac
    20701 atggaaggaa tcatgacggt tacggcaggt cgtaatatgt tatgggatct gtttattcaa
    20761 tcttatcagc aactttatgc gaagtatgga gaacaaaacg cagcgattat caaagaaaat
    20821 tgtcagacac atatttatat tatgtcgaca gatgataata ccatcgaaga attttctaaa
    20881 aaagtgggga ataagacggt tgaacaagag aacagtacca tcaattcatt aggtatgaat
    20941 actcatatca atcgcaatgt tgattcagat cgtatcttaa ctcctgaaag aatttctact
    21001 tttttagaag gtgaaacgat tgtactcgct cctttaaaac gacaagattt gaaaggtcat
    21061 aaaatacggc cgtatcctat ttttaatcat ggggaaacga atcttcctta tgcttatcaa
    21121 tatattgaag atttcaatcc atcgtgggat atcaatgata tagacataca ttgtacacat
    21181 gcggacttgg atcttaaggc tttacaaatt gattatgatt atttcctacc agatgaatat
    21241 gctagaaatc tctatcatgg actgcatgat caattagaag aaaaaaacga agaaaatatg
    21301 ttttataaaa tcgttcaatc ttttgagaat gagacggcta aaaaattgtt aattcaattt
    21361 tacgaagaac aagcaacgga tcgtcttcaa gaatatgttg aaaagttgat caaaaatgaa
    21421 gaaatttctg aaatgattag gaaacggctg ttattggaag ctgaaaaggc ggtgaaagaa
    21481 tgatcattgg aggaattttc tctggcatag ccgactggtg gtcaggttta ttcggagaag
    21541 gggatgcaga tcccaaaacc gtaacgaaag tcttacaaga ttatggtaat tatctgcagt
    21601 atcaagatgt attcagttgg atttgtaata cttttttatg gggattgatc aaagggttat
    21661 ataatctcaa tactttatta gaaaaaacaa tttatcaaag ctttgattta aaagacttat
    21721 tgaacgccgc tggtgttaat gaactatttc aaagtttgat tagtaaggta ttggctcttt
    21781 tttgtgtcgt cacattaatt tatttaggct tgaaattttt tatgagtaaa cacccaccga
    21841 aagtcaaaaa tatcttggtt aatttattta tggccatgat cttgattttt ggtggtagtt
    21901 ctttaatcga tgaagggtta aatatcagta aaaactttta tggagacgtc actgcagcaa
    21961 ataaagcaga taaaacgggt tcacctgctt ttcaaattat tcaaaataat gtgattgatg
    22021 tcagtacatt actagcgact gacccgaata aagtagaatc cttaccaaca gataaaagaa
    22081 atgctttaac agcgaaaaat ttcaaaacag cgaatatcaa tgggattatt acacctgatc
    22141 aagctaagaa aatgtctgaa tcaagtgata ttagtacagt acaaaaagat cggttaaagg
    22201 cattacagta tcgactagag ttagacgata atgggaaaga aatccctgtt caaattagtg
    22261 atgaaggttt agcaaaatat gtgtatacgt caggctatcg tcgatacatt agtagtccag
    22321 ggattatttt ggctggggaa tcttctttgt cggtagccta tttatttatt ttgtttacaa
    22381 tcgttacttg cattattgag ctagtattta aaaaattcta tttagtaatt gccgcgtcaa
    22441 cggactttga gacaggacaa cggatgaaaa gtgcgataga agatatttct caatccttct
    22501 tgttattggc gtttacgatt ttagaattac gaatctatac attaatgctt tcaggtatcg
    22561 gtgatttgca tgcggtagga aagatcaatg gattcttgta tgttgttgct ttaattgtgc
    22621 taactattgc gctattcaaa ggctctcaat cggttacgaa gattttcggt gttgatacga
    22681 gtctgaaaaa cggcagcaat tctcttctta gcatgtttgc cttaggaaat attgcgaaaa
    22741 atgtgggtag cggagctaaa aaccttgcaa gtgctggtaa gagtggttta agccatttaa
    22801 atgatattcg taagaatgga tttggccatc gaaaagatat tacccatgat aacggggaag
    22861 aacaatcaga cgataaagct acaactgtac ctaatagtaa gaagacagtt tccgaaatgt
    22921 tcgggaaagc gaaaaatggt gtttctaaag ccgcagaagg tcttggttac gtcaatgaac
    22981 gtgggttaaa aggaacagtt gaagatctta cagaaaaagc caaagacacc ttagaagaga
    23041 aattagacgg cacgagagct aaagaatttg ctgaagctgt gaagaatcca tccgaagcac
    23101 tagaaaaaca aaaagaaaaa gcttcagcag tcaaagaagg actaaaagat gcgtatggtg
    23161 aaggacaagt caatgcagca gtgaaggcaa atacaacaag tgaaaaagaa aatcaagaag
    23221 gtgcagcgat tccttcagcc aaagatcacc aagatgagtt acattctaac gaaaattcga
    23281 tcggagaaaa agattcagaa gaaggacaac atggtcagtt accaaaaacc gatccagaac
    23341 aatatcgtta taaatctaac attaattctg aaaacgaaaa acgcatttca gatttaccaa
    23401 aacgaaatca agaagtacaa acggatgagg attccatcga tctgccaaat gataatggca
    23461 tcgaagttgg aactgaaaaa tcaacgaaaa ttggtttgcc taagacacaa aatttaccaa
    23521 tgaaagatag tcgtttgaat caattcaaag agaatgatca aagctttgtt catcaatcac
    23581 ctgcaggtac tgttcaaatg aaatcaagtc gtttgcctga aaatggaaat ctgacaactg
    23641 tttcttacaa tttagctgaa agtggtgaaa cagagaatgc gtccgttcaa aatattgtga
    23701 caaaagaagc tgcacctaag ccaatcaata aagttcataa tggatacaga ccagcaaacg
    23761 tagcatttac tgcaccgaca aaaataaatg tgacaagaga aactaaaaac aggaacaatg
    23821 aattgaaaga acaactattg aaggatatca aaccataaaa ggcctattta agggcctttt
    23881 tctttataaa acaagtttaa aatggaaagg agaaaattgt ggaggatatt ttaatagcta
    23941 cgtttataac tgcactggga gcatttttaa cgatatttgt ggtgactatt aaaggattta
    24001 aagatacgga tagtttacaa aaagatcatc agtctatttt agatggtcaa aaaacacata
    24061 ccgaaagcat tattgtacac ctttcagaag gattcactta tttgaaagat gctatgccca
    24121 cattcacagt aaaattaacg gaaatatttc attctgttga gcaaatatcg tcagaattga
    24181 aagagcaaag acaacaaaaa gatcgtcaac ttggctcact taattttagg caagaaacga
    24241 tacaagaatc attggagaat gtaaatcgat taaacgatga aatgcaacga gttcatttgg
    24301 aaaatgaaga attacgcgca caaaattatg atttaattca aaagaaaaga aatctggaac
    24361 aaaaaattgt tgaattaaaa gaagaaaatc gtcaattgaa ttatcaatta cgacgaggaa
    24421 aaaatcgttc tcgtgattta ggaaatgaat tagagttgta atatttagaa aaacgctgtg
    24481 agaaatcaca gtgtttttta tttctaaaaa tacaaaatat aaggggtatg gttatattat
    24541 ggataaatac attcatatga aaaatcctcc aagatttagt ggggaatgga agtttttcgc
    24601 ctttttcttt ttagatctat taacgcttgg tggctgcttt ttttgggcga aaatagtcaa
    24661 taatcttgtc cacttaccac tcttttttgc actcttgaat tatggattat ggcttgcgat
    24721 agcgttagtg attgtttggc gaccctataa taaccctggt caacgccaaa tttttgcgat
    24781 tatcgaatct attttcttta cggataagaa ccactatcat gcgttagatc ctaaccgaaa
    24841 ttatcatggt acgtataagg aggatgaata aatgcgagtc aaaggacaaa attcagttga
    24901 taagaaatcc attttgcagt ttggaaaacc agaagagcgc tctatcttgg acttatttcc
    24961 atataaatcc tgtacagaag agggagtctt aattactaga gaagatcatt ttcagcgtta
    25021 ttatcgtgtc gtttccactg acgtggaggg attaaatgaa caagagaaac tagaacgaat
    25081 gaatcagttg accaccgtca tgcgtacgta tgtgccaaca attaagttag ttagtttgac
    25141 cacagagacg gatctaacgg aacaaatcgt gcaaaagcgg cagttattag ataaaaatcg
    25201 tatggaacag tcactaggaa gaaatctaca gcgattaaga aaatatgaaa agaaattagt
    25261 agaggagatc caggaattaa aacgggcaga acaagaacgt cctgacttaa gcttcttttt
    25321 cgttattgaa ggaaagacaa tcaaggattt acaaactaga aatcgtcagt taatgcgatc
    25381 tagtggcatt ttagggttaa aaccactcta caaaaaagaa ttgattaaga ttctttatcg
    25441 aatgaacaac atgaatgacg aataaaaaag atggaggaaa tgattcatgc tagatgtgat
    25501 aaaaacagct ttttcaacgc aaagtaatca aaaggtgatt gatccattaa aacaacttgg
    25561 taatcgtgaa atagagattt taaaatcaaa aggttatgat ttagatttct taaaacaagt
    25621 gatgccacaa ggtgggttga attttgatga agaatatgtg acctatggaa atggttattc
    25681 acgatgtgtg acagtttatc attacacaaa aaatcctact cttttttggt tggccaatct
    25741 catgaacaat cctcatacag tggccacact agatgttagc acagatgaaa agcaaaaagt
    25801 gattgatcaa atcaaccgcg cattagatga attaaatgat cgatcagata atgaacgaaa
    25861 agcgactgac cgaaataaaa gtgcggaaga atacctggaa ctagcggaat atgcccgaaa
    25921 attgacttct aatggggaaa tttctaaaca agtcaaagtg agagtacatg tttacggaaa
    25981 tacgatccaa gaagtggatg aacgggcgca ggaactgatc gaaaatctaa aagggatgga
    26041 ttatcgtgcg gtgacttatc tgcataaaac caaacgagat tggcaatcga tgtttaccat
    26101 gttcgatgaa caagctgaat ggtttggaaa tatcaaaggg caatcacttc catcgttaaa
    26161 cattggcgga ggattcccgt tccaccacca aacgctaaaa gatccaggag gcacacaata
    26221 tggggaaacc atgacaggcg gcccatttgt ctggaatgcc acgtataaaa atagtgtacg
    26281 tttatctacc aataccttgc ttctaggaat gatgggagct ggtaaatcga ccgttcttaa
    26341 aatgatcgcg gaagcccatt tagctgcagg agactttatt tggggctttg aaaagggaaa
    26401 agactttatt cctttcttaa aggaatataa cggtattatg gtgcgtttag acggatctga
    26461 cgggatgatc aatcctttag agatctttgc cacacggacg tatgatgaag catccagttt
    26521 atatgacgat ggttcagtca aagatttgaa gatcaatgaa gcagcgtcct atcaaacgca
    26581 cttagacaaa gtagtctatc aggtacagtt agtgagtcct cagctaaaag gaacgatgaa
    26641 agcagaattc aagacctact tgaatcgttt ttatgaagag tatggaacag taccacgcgg
    26701 ttttacttcc tcaaattcgc gttcaaatac tgatacgcaa gtcacaggga aagatccaga
    26761 agcttatccg acgttcaaag aatttttagc ctttttagga caactggaac taccaggggc
    26821 ttcccaagag aaaaagaatc ggaaagaaga aatggagtct atcgtggaat ccttatgtga
    26881 gacttatggc atgatttttg atggtcatag tacgattcga caccttgacc aacaacagtt
    26941 ggtctttttt gacattgact ctattacagg cctagatcga ggggtacaac aatgtcagat
    27001 gtacatggcc atgacgttga tttggaatca ggcattgatt caaggacgaa gacaacgacg
    27061 cttaattaaa gaaaaacaaa tggaagaaca agacttcaaa cgcttcttgg cgatgattga
    27121 tgagtgccat aatgcgatta atccagattt cattgaaacg gtaaattatg tcacaaactt
    27181 ccaacgagaa atgcgtaaag tagaagcaat gactgtttta gctacacaat ccccacaaga
    27241 aatggtgcca gaaaatatgt cttctgataa cttctcgaaa ctgaagaaag tctttgaatt
    27301 ttcttctacg aagatcttta tgcgtatgga tgaatctatt ttacgtcata tagaaggatt
    27361 agttggtaaa tcaatgacga actcagaatt agaatccatt cctcaacaaa atcaaggaga
    27421 cgctgtgatc aattttggct caaaagaaac cattcgtgtt cattttagtc cgaacaaacg
    27481 tcaattaaaa ttgtttgatg gaggaaaata atggaaaacc aaaatgaaag tttaatcaaa
    27541 cagtatgtta agcgtcgtgc aaagcggcgc ttatttttgt gggtgtttgg tacttctgca
    27601 ggtttgatta ctatattgat cactgtgttt gtgacccttt ttctaatact tgcggcaggt
    27661 tctattgata attctgattc aagtagtggt ggcgaagctt ttacaggaga atattcggaa
    27721 ggcttaccca tttataaaga aatcaaagga agaggccctt tttctgatga gattgcgcaa
    27781 tatgctgtag gtgcagcagt gaagtataaa ttattaccaa gtgtgatcct ttcgcaatac
    27841 ggttatgaat cagcgtttgg tacgtcggcg tccgcaagaa atgatctcaa ttattttggg
    27901 attacgtggt ttgatgggtg tctgtttcct aaaggaactg cgcgtggaat tggtggcatt
    27961 gaaggtggct ggtacatgaa gtttcccaac tcaaaagctg ccttctccta ttacggcttt
    28021 atggtagcca cacaatcaaa ttttaatgct tgtgtcggaa ataaaagccc tggcgcaagc
    28081 ttattgatct taggacgagg agggtatgcc gccgcaggaa ttactgaaga cagtgcctat
    28141 tataaaaatt gtatgtctat tattaataca aataaattga tggagtacga tgagtttgcg
    28201 attaaacact ggagagaagg cggcggaact ggtggtacga ttacaggaag ctggggcaat
    28261 ccattcccag gaagtagctt agataaaaat tcgttcagtg gaggacaact ttttgggaaa
    28321 aatccaggag gagaattccg acctaacggg ttccatgatg gactagactt tggttcagtc
    28381 gatcatccag gaagtgagat tcatgcggtt catggcggaa aagtcgtgta cgttggcaat
    28441 cctggtattt caggtttggg tgcgtgtgtg atcgtgatta attacgatgg cttgaatatg
    28501 gtgtatcaag aatttgcaaa tagtacagga aattctcgag tgaaagtagg cgatcaagtg
    28561 aaagttggtc aagtaattgg gatacgagat acagcacact tgcatttagg atttactcga
    28621 atggattggc gtcaagcaca aggacatgca ttcattgatg atggtacatg gatcgatccg
    28681 ttaccatttt tgaatagcag caaaaaatag atcaggagaa aaaatatgga aaataaaacc
    28741 ttaaaaatcg tcttaacagc tgcaattagc gttagtttaa tcggcaatat tgcgttgttt
    28801 tggcagctta aagaagaaca agagacaaat acctataaaa ttgaagaagt ggctaaaaac
    28861 gcgtctgata agctcaaaaa aaattcttct gaagcacagg cgaagattga caagttaaat
    28921 caaaccatca atgagaaaaa ccaagaaatc gatcagctga agcaacaaga tggcacaaaa
    28981 gcaaaagagg aagtaactga agcacaaaga aagacggcag aagagttcgg tcaattggcc
    29041 attgataaat cattaagcag aaatgaatta acggaaaaac taaaagatgt cgcaacccaa
    29101 gaagtgatcg acaaattaag tcctgtagac gatgatcacc aacatgatga ttacggaagt
    29161 tactctttta cattaggtga cgtgcaaact tacgctcaaa caagcagtgt aaaagaggaa
    29221 caaaattttg tcgtatttgt tgattataca atcggcaatc ctcaattcaa agatgtcaaa
    29281 ccacaaaaaa taaaaggtgg attaactgtc actgaaaaac aagtagatgg tcaatggaaa
    29341 gtcacagact ttagttattt cactcgttag gaggaagaaa atgagtaaga aaaaaggagc
    29401 aagacaacgt tttaaaaaac gaaaaagtta tcttagatgg ctggggatct ttatgattct
    29461 cggctttttg attcttgcta gtccgattta cacacataaa aaggtagaca taccagatga
    29521 agtaagcacg aacgcatttt taaacacgaa ggaactgacg atggtttcaa aagaaaaagg
    29581 agcgaaccag caattagttt ttgaatttgc gatcgacgat gaagatcaga gcttattaaa
    29641 agaactagct aatcttaatt atcgtgtaga agtaaagaca gctaaagggg attatcaagc
    29701 aataaaagcc aaagtgatca aggtgtctga tgattatttt gtagtgaagt tagcacatgt
    29761 accagaagaa tatattgcta tgcgattaac tattattcct gaaaaaattg atccaaaggt
    29821 ggatatgcaa gaaccgcaag atctgatttt ttatattcac gaagataagg tcaaagatca
    29881 cgtgaaagac gaagattatg aacaacatgc gatcaattac aaagtgaaag gatacaagaa
    29941 agaacaaaaa gcagcacaaa aacaaattga atccttccaa gcaaccattg atttgaatga
    30001 agaattagtg aagaaactaa aagatcaatt gccttatcaa gtcgcagaag atcaggaaaa
    30061 tactgaaaat aagattaata gttaccagca agaaattgaa acatcgaaaa cgcaaatgaa
    30121 agatcaagaa gagagcattc aaaaacttca agaaaaaatt gatcgtatga caaaagaaaa
    30181 tatttagaag aataggtgga gtaaaagaaa tgaagcgagc gaacttggtc ggcaaagata
    30241 ctttcgctga gcagaaagta ttgattaaag cctatctcaa agttgtaaaa gctaaagaaa
    30301 tatgtcaaga agtgaattat ctaacacaac aatatctgag ttgaagaatg gtagaaagag
    30361 tttagatacc ttaaaattca gatcatttga agctctatat atagctgcta aaaaatattt
    30421 tacagctgat aataaataaa aaattaaggt gggttctatg tgaaaaaaac gtatcaaaag
    30481 tttttatgga ttagctatat cgtcctattt atcgttgggt ttctcgtcag tgtgtccgtg
    30541 atcgtcggaa cgctttttct tttgttctct gacgaagatc tggtactt
//
LOCUS       DACEXQ010000011        30487 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.011, whole genome shotgun sequence.
ACCESSION   DACEXQ010000011 DACEXQ010000000
VERSION     DACEXQ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.011"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..105
                     /locus_tag="IXH04_000360"
     CDS             <1..105
                     /locus_tag="IXH04_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1850994.1"
                     /translation="SGESQNKVILWSGIILLSIATMLSAKRFKQNRSL"
     gene            complement(231..1310)
                     /locus_tag="IXH04_000361"
     CDS             complement(231..1310)
                     /locus_tag="IXH04_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1850995.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(1413..1853)
                     /locus_tag="IXH04_000362"
     CDS             complement(1413..1853)
                     /locus_tag="IXH04_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1850996.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(1910..2320)
                     /locus_tag="IXH04_000363"
     CDS             complement(1910..2320)
                     /locus_tag="IXH04_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ1850997.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(2500..2934)
                     /locus_tag="IXH04_000364"
     CDS             complement(2500..2934)
                     /locus_tag="IXH04_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ1850998.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(3034..3882)
                     /locus_tag="IXH04_000365"
     CDS             complement(3034..3882)
                     /locus_tag="IXH04_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1850999.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            4271..6037
                     /locus_tag="IXH04_000366"
     CDS             4271..6037
                     /locus_tag="IXH04_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAQ1851000.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            6139..7398
                     /locus_tag="IXH04_000367"
     CDS             6139..7398
                     /locus_tag="IXH04_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ1851001.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            7545..8846
                     /locus_tag="IXH04_000368"
     CDS             7545..8846
                     /locus_tag="IXH04_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1851002.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEDIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            8849..9700
                     /locus_tag="IXH04_000369"
     CDS             8849..9700
                     /locus_tag="IXH04_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1851003.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            9713..10528
                     /locus_tag="IXH04_000370"
     CDS             9713..10528
                     /locus_tag="IXH04_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAQ1851004.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            10679..11845
                     /locus_tag="IXH04_000371"
     CDS             10679..11845
                     /locus_tag="IXH04_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1851005.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPTQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            12075..12722
                     /locus_tag="IXH04_000372"
     CDS             12075..12722
                     /locus_tag="IXH04_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAQ1851006.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            12806..13750
                     /locus_tag="IXH04_000373"
     CDS             12806..13750
                     /locus_tag="IXH04_000373"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAQ1851007.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            13885..15336
                     /locus_tag="IXH04_000374"
     CDS             13885..15336
                     /locus_tag="IXH04_000374"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAQ1851008.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            15357..16193
                     /locus_tag="IXH04_000375"
     CDS             15357..16193
                     /locus_tag="IXH04_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ1851009.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            16193..16852
                     /locus_tag="IXH04_000376"
     CDS             16193..16852
                     /locus_tag="IXH04_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ1851010.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            16978..17667
                     /locus_tag="IXH04_000377"
     CDS             16978..17667
                     /locus_tag="IXH04_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851011.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            17673..18629
                     /locus_tag="IXH04_000378"
     CDS             17673..18629
                     /locus_tag="IXH04_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAQ1851012.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            18673..19185
                     /locus_tag="IXH04_000379"
     CDS             18673..19185
                     /locus_tag="IXH04_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1851013.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(19441..19989)
                     /locus_tag="IXH04_000380"
     CDS             complement(19441..19989)
                     /locus_tag="IXH04_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1851014.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(20078..21259)
                     /locus_tag="IXH04_000381"
     CDS             complement(20078..21259)
                     /locus_tag="IXH04_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAQ1851015.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(21495..23003)
                     /locus_tag="IXH04_000382"
     CDS             complement(21495..23003)
                     /locus_tag="IXH04_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAQ1851016.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            23162..23353
                     /locus_tag="IXH04_000383"
     CDS             23162..23353
                     /locus_tag="IXH04_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851017.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            23556..24473
                     /locus_tag="IXH04_000384"
     CDS             23556..24473
                     /locus_tag="IXH04_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAQ1851018.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            24466..25539
                     /locus_tag="IXH04_000385"
     CDS             24466..25539
                     /locus_tag="IXH04_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851019.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            25687..26205
                     /locus_tag="IXH04_000386"
     CDS             25687..26205
                     /locus_tag="IXH04_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851020.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            26186..26578
                     /locus_tag="IXH04_000387"
     CDS             26186..26578
                     /locus_tag="IXH04_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAQ1851021.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            26654..27589
                     /locus_tag="IXH04_000388"
     CDS             26654..27589
                     /locus_tag="IXH04_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAQ1851022.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            27722..28501
                     /locus_tag="IXH04_000389"
     CDS             27722..28501
                     /locus_tag="IXH04_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAQ1851023.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            28494..28811
                     /locus_tag="IXH04_000390"
     CDS             28494..28811
                     /locus_tag="IXH04_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ1851024.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            29055..>30487
                     /locus_tag="IXH04_000391"
     CDS             29055..>30487
                     /locus_tag="IXH04_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ1851025.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAV"
BASE COUNT         9970 a         5452 c         5965 g         9100 t
ORIGIN      
        1 tccggagagt cacaaaacaa agttatactg tggagcggaa tcattctatt aagtatagca
       61 acaatgcttt ctgcaaaaag atttaaacaa aacagatctc tctaacattt gaataatgtc
      121 tgtgaacgca tttttcaaat aaaaagaaat atccagaaaa tgaccccaaa ataccagatt
      181 gaaatctgat atttggggtc gttcatactt tatctttctt catatttatt ttaagggata
      241 tctgtttctt ttggaagatg gttcgggatc tttttcactt tcaatacatc taaaaagaag
      301 gtaaagatcg gaatgccgac aatcaagccc caaacgccaa agaaccgttc gctaacaagt
      361 aagaccacaa atgtataaaa aatcggcagt tctgttttgc tggacatcaa cttcggattc
      421 agcacataag actcaattag atggacaatc aaaatcgtaa tcaggatata aacaacatct
      481 cggatgcctc cttgggaata agctatgaaa gatagaggaa tacaagaaat gataacacct
      541 gctactggga tcagacttaa aatgaaaatc atgatcgcta aactcagcag ctgatgaaag
      601 ccaaatacag ccaaggcgat tgtagtgagc aacgtattta aaagagcgac aacaaattga
      661 gcttctaata ccaaaccaaa tgtattgaca aacttatcgg caaaatagta aatatcttga
      721 aagaaccaag aaaattcact ttttaaaaac aatcttgaga atacaattgt ttttttcttt
      781 tcaatcataa aaaagaaact taagatgaat gacatagcaa acgccactgc catttttcct
      841 atatcctgta aatagccaag cgcaatcgaa gcgccatttt gaagctgttc aaaaaaatcg
      901 tttcgctcaa tataagtatg gatatattgt aaaacaggat ctgcatcttt tggctgatgc
      961 tcgtaaaaat taataacgga ctggatcatg tcaaatgttt gatgaataag catcgggata
     1021 tactttgtga atgccagata gataaggtaa acgatcagtg tataaatcat gatagctaaa
     1081 acacctgtag ggattcggac aaatcgctga atgatttgaa caagatgaat catcaaataa
     1141 gtaaagataa atgtgagcaa aatggttgta atcatacttc taactaagaa aagaacaaaa
     1201 acgacaaata ataaaacagt gagacggcgt attttttcat tcgccaaaaa acgctgataa
     1261 acggtcaaat cgacaacctc caaataacaa aaaataaaca cgttattcat tatatgatgg
     1321 aatatgaaaa taagaaagag caaaaagaaa aaccgctaaa tgacgttatc ttatgagttg
     1381 gttttcctca taaaatcgtt catctagcag tttcagccct ttttctggtc ttgatgaaaa
     1441 ttatctagca tttttcgggt cagatgttgc agtgtatcca attcttcctg ttcaagtgga
     1501 agataagact tgatcttttg tggaatacct tctgcttgtt tttgcaacgc agcaccttga
     1561 gcagtcagcg taacatttac gattctttca tcacccgttt cacgctgacg attaatataa
     1621 tttttttctg ctaacttttt tagtaaggga gtcagcgtac cagaatccag acacaaatca
     1681 tttcctagct ctttcactga agtctttttc ttttcccata aaactaacat cacaagatac
     1741 tgggtatagg taagattcag cggcttgagg aggggagtgt agtgtctaat gatttcttta
     1801 gaggcggcat ataaaggaaa acatacttga ttaactaaat atagatcttc catttttcca
     1861 cctccgctgt tttactatag tgtatcatag cggatttaca caaaaaagtc tattcactaa
     1921 ctgctttcag agtaacttct atatttccag ctgtagcttt tgaatacgga cacactttat
     1981 gcgctgcctc taataattct tgcgtttctt ccagagaaag accttcgata gatccttcaa
     2041 tctctacacc taagacaaag ctcgtttcac tttctgaata aagggaaact tgtgcactga
     2101 cagtagaagc cccagagata tttttttgct gtttgatcaa ttctaatgca ctgttaaaac
     2161 atgcactgaa accagctgca aacaattgtt cagggttggt tgcattttcg acgcgtttac
     2221 caggagctgc aatattcaat tcgaatgtac gatcgggatt atgcacttct ccagagcgtc
     2281 cgcctgtgtt gatcattttc gtagaataca tttttttcat aaaattacca cctttaatta
     2341 tattttatac aattgaattt tacacaatat aattgtggaa agcaaagaaa tggattggca
     2401 taagataaat aagagaaagc acatgaaaca gacgcgcttt ctcgaatagc aaacatatac
     2461 aaaaaatatc agagacaaaa aataatctca tcataaacat tacatatatt tctcaaaaag
     2521 ttgttttctt tcttcaaaaa ttacttcacg atggacctga tgaagcatcc ataaataacc
     2581 aaaaggatct ctgaacattg agttagtcac accaaagtcc atcatttctg tcatttcttg
     2641 aatctctaca cagccatttc gtaatgcttt attatgtgtg tcctcgatat cagagacgat
     2701 gatattgaac cagatcgtat ttggatgttc agcagtagga gcagtcaaac caagagacgt
     2761 gttttcatcc agcatatgga atctcgtacc atacaaggta aaaatgactt cattattgcc
     2821 tctagggtaa tctgttgcct caatcagttg tatttcatca aagattgttt gatacagttc
     2881 tagtgctttc aaactatcat ttaatacaaa atcgatttcc gttcctttat gcataaagat
     2941 ctcctctcct tttcagtcga acagttacta gattctttta ttataccagc taaagaaaac
     3001 gcattcaaaa aaatagcgga tttacttacc aactcattcg attggatgat aagtaaatcc
     3061 gcgtcccatt acttctgcta cgctgctgac aatgacaaag gcacttggat cgatttcttc
     3121 tatcaatggt ttcattcggg aaaattcatg atcagtcaca acgatcaata aaacctcttt
     3181 tgactttcga ttatacccgc cttttgcctc tagcagggta aggccacgtc caatctcagc
     3241 acttaagcga aggcggatgt cttccaaata atgttcactc atgaccataa cagatttctt
     3301 tcgattgata cctgtttcga tataagtcat gacaattgaa gtgattgcaa tagataaaat
     3361 agcataaaga aactcttcta ttccaaaaat aaacagatta agagaaacaa cgaatgcgtc
     3421 tgtaaaaaat aagccaatcg aagtattcaa gtggaaatat tttttaaaaa tgaggggagg
     3481 gatcgtcgta ccgccgctag aagagttgtt tttatataaa atcgcaactc ctaaagcaaa
     3541 aatcgcactt ccaaaaatga cagacaacaa ccgatctgaa gtaatcattg tttctggaac
     3601 tattgcaatg attaaaggaa aaaccaagct tccgaaaagt actttaaaaa agggtttttt
     3661 acctaaaaat agaagagcta gtaccaacat aacgatattc aacactaaaa taactatagc
     3721 tcggtcaaag cctaaagcag cttccagtaa tattcctaga ccgctgacac ctccagcagc
     3781 aatatgatga ggccctaaaa acatattgat agcgactcct aatatgaaga gggcaaaaat
     3841 aatcttaatg tatgtccata taaattgtct cgtttctttc atttattcac ttccttaatt
     3901 cattaataat actcttatct tattataact tacttttcac cttaagttaa ctttaattaa
     3961 taaaaaaatt aggctagtct aaattattat gtttcattct tctagaatat acttctacgc
     4021 caaaaactgt taaaaaactt ttactttaga aaattggaga aattgatcgc ttaagataaa
     4081 ttcaggacac taaatagtac aggaagtaga ctaaaacttt taaaagtttt aagtctatct
     4141 atttggcaag tttaaaaaat aaaaattcgg tatcaaaaaa ctgttaccga taacattttt
     4201 gaaagcgttg tcttttgctt ctctatcact tatagtgatg acgtcatatt aaaacacaag
     4261 gaggaaatca atggatacag ctgcaattta tcatcgaccg gaaagtgaat atgcatttct
     4321 atatacgaat acaagatttc gtattcggct tcgtactagg aaaaatgata tcaaaaaagt
     4381 atatgtgcta tgtggagatc catataccat aacaactgaa aaatggtatc aaaaacaacg
     4441 accaatgaaa aaatgtctaa gtaccaatgt tcatgattat tgggaaattg aagtgacgag
     4501 cgaaacaagt cgcttacagt atgcgttcca tgttgttggg gaagataaca cggactgttt
     4561 ttacggtgat caaggaattt ttccttatcg agaaaatgtg attactgaac caaattttta
     4621 ttttcgaata ccttattttc atcaaatcga tcgattcact attccagaat gggtaaaaga
     4681 aacagtatgg tatcaaattt ttcccgaaag atttgcaaac ggggacaaag caaatgaccc
     4741 cgaacacact ttaccttggg gaagtaaaga tccagaccga gaagactttt tcgggggaga
     4801 tttgcaaggt gtgatggatc atttggatta cttgacggac cttggcatca atggcatcta
     4861 tttttgtccc atatttaaag ccacatccaa tcataaatac gatacgattg attattatga
     4921 ggtggatccc gcttttggcg ataaaaaact tttaaaaaac ttgattgatg aagcacataa
     4981 aagagggata cgtatcatgt tcgatgctgt attcaatcat atgggtgtcc attcaccaca
     5041 gtggcaagac gttctcaaaa acggagaaaa atcgatctat aaagactggt tccacatccg
     5101 ttttttccca gtagattctt atcagatgac tgacttacca gaaactgctg aaaatatccc
     5161 atatgatact tttgctttta ctccattcat gccgaaacta aatacagcaa atccagaagt
     5221 gcaaaaatat ttgctggata tcgcaactta ttggataaaa gaattcgata ttgatggatg
     5281 gcgtcttgat gttgctaatg aagtcgatca tcatttttgg aaaaaattca gagaagcagt
     5341 tacagaaatc aaaccagaca tctatatcct tggagagatt tggcattcct ctcaagcttg
     5401 gctgcaaggg gatgaattcc atgcagtgat gaattatgca tttacagatt cgattaaaga
     5461 ttattttgca aaaaagaaaa tcacagcttc acaaatggtc agtggaatga atcatcagca
     5521 aatgctttac agagatcagg tgaacgaagg aacattcaac ttattagatt ctcatgatac
     5581 tgccaggatc ttgacccttt gccaaggtaa caaagaattg atgaagtctg tgatggcctt
     5641 tatgtttctt caaaaagggt ctccttgtat ctattacggg acagaaatag ggatgactgg
     5701 tgaagatgac ccggattgcc gcaaatgtat gatatgggag aaggaggaac aagatctaga
     5761 gctattcggt tttataaaag aacttgtttc tctaaggaaa cagttgtcta agattatttc
     5821 tgaaggttct actcagtggc taatagtaaa tgatcgtgaa gataagctct atttcacacg
     5881 gaaactagag ggacagatca tctatgttta ctttaatcag tcaaaagaac cttgggtagt
     5941 agaacaggaa aatgaggtga ttcttagtca aaattgtcag ttgttagaag acgggaaagc
     6001 agagatacga caatacggat ttttgatcat gtgttgaaaa ctgttaccgg taacaacaag
     6061 aaagcgtttg caaatgaaga tttgtgccca ttataataag ggtgtaacag cacgttgaat
     6121 acaaaggagg aaatgaagat gaaaaaaaga agtgtgaaac agttgggttt agggttaatc
     6181 tctttaggaa tcttaggcgg attagcggct tgtggcaatg gtaactcttc agcagaaggg
     6241 gaagccgatg aaggaaaaac gttaacagtt tccgttgatt ctggatataa agattatata
     6301 aacgaaataa aaaacacttt cgaaaaagaa aacgatgtga aaatcaagct agtagaaaag
     6361 gacatgttcg atcagttgga atcattagcg ttagatgggc ctgctggaaa gggacctgac
     6421 gttatgatgg cagcatatga ccggatcggt gcgttaggac agcaaggaca tctggcggaa
     6481 gtaaaactgg gaaatgaagc agcttacgat gaaacagaca aagcccaagt gacttatgat
     6541 ggaaaaatct acggagaacc tgcagtaatc gagacccttg ttttgtacta taacaaagat
     6601 ttagtcgata cagcaccatc aacgtttaaa gatttggaga atctatccaa agacagtcgt
     6661 tttgcttttg agtcagaagc aggaaaaaat acaggatttt tagctaaatg gactgacttt
     6721 tattattcct atggtttaat tgcaggttac ggtggctatg tatttggtga tgatggcaca
     6781 aatccttcag atatcggttt gaataatgca ggtgctgtag aaggaatctc ttatgcaaca
     6841 gattggttca aaaatgtttg gccaaaggga atgcaagaca taaaaagtgc aggagacttc
     6901 gccagccagc aattcatgtc caataaaact gcagcaatca ttgacggtcc atggcaagcg
     6961 caaacataca aagaaaacaa tatcaattat ggtgtagcca aaattcctac tctaaataat
     7021 ggacagcctt atcaaccgtt tggcggagga aaaggatggg tagtcagcaa ttatgctaaa
     7081 aacaaagacc tctctcaaaa atggcttgat tatgtaacga atcaggaaaa tcaagaaaaa
     7141 ttctttgaaa tggtaaatga aatccctgca aaccaacaag ctagggaaac agcaaaggct
     7201 aaaaacgatg aattaacaac agctgtgatt gaacaatatg aaacagctca agcgatgcca
     7261 aacatcccag aaatgggcga agtttggaca ggtgcagaaa acttgatgtt cgatgcagca
     7321 tcaggttcaa aaacaccaaa agaatccgca gatgaagcag taaaaacaat cagtgaagcc
     7381 atagaacaaa aatactgatt aaaatgataa ccgtccattt ttataaaaaa tggaacaggt
     7441 gcttcagata aaagtggcaa gagcgagatg gaaaattatc ttgctcttgt tgccattatc
     7501 ttaaaagcag tttgcggttt agcaagttta gagggggcat tcagatgcag caagataaaa
     7561 caagcaatat caacgaaaaa aaaatcacgc tttgttcact tattccaggg atggggcagt
     7621 ttgtaaatgg ccaagctttt aaaggtatcg tttttttcgc catattcgtc ctattcattg
     7681 gtcagatgct tctgggcgga tttcatgcat tggtaggatt ggttactttg ggaagtatac
     7741 caatggaaga tcattcatta tttattttga ttgaaggcac tttgcaattc atcattacaa
     7801 tcattttctt gattttttac ggtttgaatc ttttagacgc ttatcgagta gcgaagatgc
     7861 gtcaagaggg caaaaaaatc aatcggacga tgaaggaaga catacataat ataggtgatg
     7921 aaggttttcc ttatctattg acacttcccg cttatcttct catgattttt acgatcattt
     7981 tcccagtcct agttacattg ttcactgcat ttactaatta tgacttcaat catattccac
     8041 cagccagttt aattgattgg gtaggggcta aaaacttttt tagtatcttc tttttaagct
     8101 catatcgtaa tacttttttt gcagtctttt cgtggaccgt gatttggact ttatgtgcaa
     8161 caactttgca aatcaccctt ggtattctca cagcggttgt tgctcaccag aaatttatta
     8221 aattcaaacg tattttcggt atcattttct tattaccatg ggcggtccca gcttttatta
     8281 ctatcatgag tttttcgaat atattcaacg atagcgtagg tgccatcaat actcaagtga
     8341 ttcctttcat aaaccagctt ccttttgtag atatcagtgc aatctcttgg aaaacggacc
     8401 cgaactggac gaaagtagca attatcttag tgcagggctg gttaggattt ccctatatct
     8461 atgtgatggt tacaggaatc ttacaatcga tttcagaaga cttatatgaa gcagcaaaaa
     8521 tcgacggagc aaatgcatgg cagcgctttt caaacatcac gcttcctgct gtcttcctag
     8581 tggcagcgcc aacatttatt acacagtata cggggaattt caataatttc tccatgatct
     8641 atctgtttaa caatggcggt cccggaagcg taggcggagg agctggttct actgatattc
     8701 ttatttcctg gatctataaa cttacgactg gaacttctcc gcagtattct atggcttcag
     8761 caatcacgtt gattatctcc gtcatcgtta tctgtgtgtc attgctggta tttaaaaaga
     8821 caaatgcgtt caatatgggg gattaacaat gaaaaaattt cattcgagtg ctcgattcca
     8881 aaaacgactt tctcactttt ttacttactt ttttctttta atactgtcag tcattatcat
     8941 ctatccgctt ttgatcacag caagtactgc cttcaagtca ggaaatattg cagcttttag
     9001 tttagatctt tcaagtgact ggactttagg aaactttact cggctattca gtgaaacact
     9061 ttacgggact tggtataaaa atacattgat catttctgtc ctaacaatga tcgtacaagt
     9121 gactgtagta actttggcgg gctatactta tagtcgctat cgttttatcg gccgcaaatc
     9181 aagtttgatg ttctttttag tgattcagat ggtaccgact atggcagcat tgactgcctt
     9241 ttatgtcatg gctttcttat tagaagcgct cgataagtac tggttcttga caatgatcta
     9301 tataggcggt ggaattccaa tgaatacttg gctgatgaaa ggatactttg acactgtacc
     9361 aatagattta gatgaatctg ctaaacttga tggggcaggg catttcagga tttttttcca
     9421 gattgtatta ccgcttgtca aaccgatgct cgccgttcaa gcattatggg cttttatggg
     9481 accttttggg gatttcatgt tagctaagtt cctgcttcga acacctgaaa atcagactgt
     9541 tgctgtcggc ttacaaacat ttatttctga tgtgacaaac caaagagtct ctctttttgc
     9601 tgctggggca attttgattg cactaccgat ttgtctatta ttcttctttt tacaaaagaa
     9661 ctttgttacc ggcctgacat ctggcggaac aaaaggatag gagcagagta tcatgcaaac
     9721 atttccgtta aactattttt ccgcattacg tacgccaact caagtgtttg cgggacgcaa
     9781 actattatca tggcctaaat ttttcttgat atttgtattt ttagtttcct tgatggtcat
     9841 gcctgtgact cttttttatg ccaatcagat ccaagcgatt ccattagaac aatttctatc
     9901 cgttcattcg ctgatcgatg aacaaggaac ccaaaaattt tctgaacttg aattatctga
     9961 aactgggtta caagcatcgc aacaaacgat agctgtgacc cctgaaattt tggtaggtgt
    10021 gtctttatct gagaaacaac aatccgatca tggaactttt attgattttg agaaggaaca
    10081 atgggtgatc caacaaaagg ataaatctgg tatacgccgc tatacgatga attatagccc
    10141 atctttccaa ccagattctg tacgtacgcc cgaagatttc caacgatttt tagaaagaga
    10201 attttatgcc agtaatcgtc caacaatcat tttatcttat agtttaagtc ttggtcttgt
    10261 cctatttgtc atgacgagtc tcattttatt cggggcttcc tttttcttgt ggatgacaag
    10321 aaaaagccaa ctttcatcca ttcatacatt caaagagagt gcaaatttga tgctgaatgt
    10381 tatgggaata ggttccatgg cagcagcagt tgtagggttt atccactttg actttatctt
    10441 gatgctaagt gtccaaacaa cgataactgt cttgctgctt ttatgggttt ttgtcaaaac
    10501 aagattcaaa gataagcgtg tcgaatgaca agtttaagcg atttatgcga caatgaatgt
    10561 gaaaaaaatg aaaaataaag tagtttttga aaaatttctg aaaaaattaa acttcttagt
    10621 tgattctttt cttttttagt attaacgtaa tagggtagtt tttttaagga ggaaaatcat
    10681 gttagagaaa actttataca atcaattatt ttcacgttct ttctcgctcc ctgtcgaagt
    10741 aaattattgg gacggtacaa cgaaacaata cggagatacg gacactccta cccaaatcaa
    10801 aatcaatatc catgaagaaa tcccaatgaa agagattaca agtaacgcat ctttggcact
    10861 aggagaagcc tatatggatc atcgaatcga gatcgaggga agtatccaag cattgatcaa
    10921 cgatgcttat cagcaagcaa acagcttttt acgaggaaaa gattatctaa aatggctgcc
    10981 aaagaaagaa aaacatacga aacaaaaaaa caaagaagat atccatgcgc attatgactt
    11041 aggtaacgac ttttataaac tttggctaga tccaactttg acttattctt gtgcttattt
    11101 caatacacct gaagatacgt tggaacaagc acaaatcaat aaagtccatc atatcttaga
    11161 caaactcttt atcaaagaag gcgaaacgtt gcttgatatc ggttgtggct ggggaacatt
    11221 gatgttcact gctaccaaag agtataatgt caaggcaact gggatcacac taagtgaaga
    11281 acaatacgat ttgattacca aaagaataaa agaagaacat ttagaagata aatgccgtgt
    11341 attattaatg gattaccgcg aactaaaagg cgaaacgttt gatcatatca caagtgtagg
    11401 tatgttcgaa catgtaggtt ctgaaaatct tgaaggttat ttcaaagtgg taaaagattt
    11461 attgaaacca aaaggaacag ctttgatcca tggaatcagc cgacaacaag gcggtgcgac
    11521 aaatgcttgg atcaataaat atattttccc tggcggttat gtccctggtg ttactgaatt
    11581 agttgataat attacaaaaa acgatttgca actgattgat ttggaaagct tacgtcgtga
    11641 ctaccaattg acattagaac attggacaaa gaattttcat caagtccaag atcaagtcac
    11701 gaaagaaatg ggtgattcct tctatcgaat gtgggatcta tatttacaag cttgtgcagc
    11761 ttcatttgaa tcaagtaata tcgatgtgat tcaatatttg cttgttcaac ctggaaacaa
    11821 tgacatccca atgcatcgtg cataattgag gcaaaagtac tgtttaaaga ctatttaaaa
    11881 agaagctaag ttttatgatg ctccagaagt gaactttttt gtctaacttc taggattgtc
    11941 tctaaagctt agtttctttt tctttatatt ttcataataa aagtttggtt ctttaatttt
    12001 taaacatgac agaaaatttt ttcttcgtta tacttgtaat agaatactat tttataacaa
    12061 aggggatcga atcaatgaaa aaaactgtta tttatactag tttatttgca gctttgtttt
    12121 tagctgcttg tcaatcaaat accaatcaag gcacatctgg gaatacgtct tccagtagcg
    12181 aacagcgctc ttcacaggta tcggactcct caacaaaagc aagcacatct tcgtctagcc
    12241 agacaaaaag ttctagtgaa gcaagtactc agtcagatat caaagtgtct gcagctcgtg
    12301 cgatcgaatt gttccaagat aaatacccag atactgcaat tactagtttg gaattagata
    12361 gtgattgggg atcttacttc tacaagatag aaggtgtcga tgatcaaaaa gaatacgaag
    12421 caagaattga tgctcaaaat gaaaaaatgg agacagagaa tccagaaact ctagatcgag
    12481 atgaacaaaa tggtcaaaag aaaaaagaag atggtctgga tgttgataaa ttgatcacga
    12541 ttgaggaagc agcaaaaatc gccgtcgaca aagtaggaag cggtacggca acagattggg
    12601 atttggataa agatttaggt actacttatt gggaagtcaa agtaaaagac ggcaatcaaa
    12661 gtacatcagt caagatcaat agccaaacag gcgaagtact gtcaactgaa caagatgatt
    12721 aagaatttct tcggaaaaca agagttgatt tttcttttag tcatgataca ctagacaaaa
    12781 taacttgttt gaaggagcga tttacttgag tttagaaacg acctttgcca atcatatctt
    12841 tgccaatcct ttgatgaatg catctggtgt ccactgcatg actacccagg aattagatga
    12901 actggctcat tcagaagcag gagcatttat cactaagagc tgtacgatca atgaacgaaa
    12961 aggaaatccc gaaccacgct attttgatgt tcctttaggg agtatcaatt ctatgggact
    13021 tccaaactta ggtttttctt attatctaga gtatgctctt gcgtatgaaa aagtgcagga
    13081 aaaccaaaat caaccgttat ttttttccat tgccgggatg agtgtccagg aaaacttgga
    13141 aatgcttgag aaaatagaaa aaagtgggtt taatgggatc actgaattaa atttatcttg
    13201 tcccaatgtg cctggaaaac cacaactagc ttacgatttt gaggccactt atgaaacact
    13261 gaaagaagta ttcagcatct tttctaagcc gctaggaatc aaattgcctc cttattttga
    13321 ttttgctcat tttgatcaga tggctgatat cttgaatcag tttccattga cctatgtcaa
    13381 tgctatcaac agtgtgggca atggtttgta tatcgataca gaacaagaag cagtagtcat
    13441 caaaccaaaa gaagggttcg gcggcattgg cggagagtat atcaagccga ctgctttggc
    13501 gaatgttcgc gctttttata cacgtttgaa accagaaatc caaatcattg gtacaggtgg
    13561 gattcggaca ggacaagatg catttgaaca tctgctatgt ggcgcaagca tgttacagat
    13621 tggtaccgag cttcataaag aagggccgga aattttttct agaatcataa aagaattaac
    13681 gcagattatg tcagaaaaag gatatacgtc aattgacgaa tttaaaggca aattacgcac
    13741 catttcatag caaagagacg aaggtcatga ttgatcttcg tcttttttta taaaaaacac
    13801 tcgtctgttc attcctgtgc aagcgtactc attgtatgtt agacgaactt gtgggaaaat
    13861 aacagaggca aaaaggagga taatatgaat gatgcaacag aattagcaaa agagcttaaa
    13921 gaaaaagaat ttagttttca agaactagtt acggatatac agaaaaaaat aaagaagcaa
    13981 aatcatgaat tgaatgcgtt tgtcgcgctt gaaccattcg agagtaattc ccacgagcta
    14041 ttgattcccc agtcagaaaa agggccattg gctggtatcc catttccttt aaaaatgctc
    14101 ggtcaagaaa aaaagggatg gctagctact tcaggatctc gtttatttga aacacaccga
    14161 gcttctcata catctaatta tgtgcggcaa gcagaagcga taggtctagt tccttttgga
    14221 caaaccaatg cgccagagtt tggtttcaaa aatattacag acccagtcat ctatggccca
    14281 gcaagaaacc cttggaacct tgaatgtaca ccgggcggat caagtggagg agctgctgct
    14341 gcagtttcat caggaatcgt tcctttagct ggtgctagtg atggcggtgg ttctattaga
    14401 atacccgctt cgttttgcgg attgattggg ctgaaacctt cgcgagggac gatgccagtt
    14461 ggtccatatg cttggagagg atggcaagga gctgctattg atttcggctt gacagtttct
    14521 atgagagaca cggaggcttt attttatggg atgagaagta tccattccgg tgcgccatat
    14581 caagtatcgc cagttgagtg gcaaacacat ccaagaaaaa aacggttgaa gatagctttt
    14641 tgtgttgatt caccgatcca ctcacagatt agtgaagagg cagtacttgc tgtaaaaaaa
    14701 gctgtagatt tccttggaaa agaagggcat gatttgacag agattcctta cccactagat
    14761 ggtcgtaaac tgatcacgtc ttattatcag atgaatggcg cagaaacagc agcaatgatc
    14821 cgctcgattg aaacaggcat ccagcgagct gtcagaaaag aagaacttga acccatttct
    14881 tggactttgt tccaatatgg tcagaagctc tctgccgcta cttacatcga ttctctgcat
    14941 gtatgggatg aagcagcaat tgtcatggaa aacctacatc aagaatacga tctattcctt
    15001 tcaccaacga ctgcatttac cgcccctaaa atcagcgaag atttgcaaag tgatgcgata
    15061 agagcacaga tggaacacat cagtgattgt tctgaagtag aaggtttaga cgttgtttct
    15121 tccatgtttg aggagagctt gaagatcaca ccttatacac agttggctaa tctgactgga
    15181 caaccagcaa tcagcttgcc tactcatttg acaaaagaaa acatgcctct aggaatccaa
    15241 tttatggctg ccagaggaag agaagatttg ctatttcaaa tcggctatct atttgaagca
    15301 aatcatcagt tccatttacc aaatccatgg aaatagcaga aacaaggaga agaagaatga
    15361 agaaaaaaag attgtttgct ctaataacta gtgccttttt tattttaagc gcttgcggta
    15421 cagctgccgc taatcaggaa gataacagct ggagtaatgt agaaaaaaag aaagaattag
    15481 tagtagcgac ttctggcaca ctgtttcccg catcttatta taatgatcaa aatgaattag
    15541 ttggttatga tgtagatttg gcaaaagagg tggctaaacg gttaaatgtc aaaatctcct
    15601 ttaaagaata caacgtggat ggacaaatca cttctgtttc aaaaagagaa tcagattttg
    15661 ctgcgaacga ttttggtatc acaaaagaaa gagaaaaaaa attctccctt tcagagactt
    15721 tcaaatattc atttgacagt atgatcgtac gaaaaaaaga tgattcagga atccactcac
    15781 ttgaagatct taaagggaaa aaagcagctg gcgaacccaa tactagctat atgcgtttag
    15841 cggaaaaaca cggagctaaa ttagtcacat atgacaatgc cacgaatgat cagtatttaa
    15901 gcgatgtagc aaatggtcga acagatgtta ttctaaatga ttattatctg cagaaaatgg
    15961 ccgtatctgc tttaccagat atccctgtaa aaatactgga gaacgtttat ttcaatgaaa
    16021 acgaaagtgg ttttttattg aaagaaggaa atgatgctct gaaaaagaaa atcgatggcg
    16081 tattaaaaga gatgaaagaa gatgggacga caaagaaaat atctgaaaaa tattttcaaa
    16141 ctgatgtttc agtcaaaccg aaagaaacga tggctgagtc ggtgagtgag tgatgtactt
    16201 tcctctaatt gatttttccc tcatgtggac ttcacttcct ttcgtactgc aaggcttggt
    16261 atacacatta gggattggct tcgtgtcgtt tcttttagga aatctggtag ggttgctttt
    16321 gacagtttta ggtcttttgg attggctgcc tctgaatgta tttattcgtt tttatttatc
    16381 gttctttaga ggcattcccg cactagtcct gctgtttctg ttatattttg ggttacctta
    16441 tcaattaagt gccttaacgg cttcagtcat ctgctttacg attaccagca gtgcatttat
    16501 tggtgaaatc tatcgaggat cactagcagg cgtttcttca ggtcagtggg acgctggttt
    16561 agcttctggt ttttctttct ttggggtatt gaaatatatc gttttgccgc aagcttttcg
    16621 aatctcagta ccggcactca gtaacgtagc tatggatcta gtgaaaggta cttcgttagc
    16681 agcaacaatc acagtgccag aaatatttca aaaagcaaag atcataggcg gtcggacatt
    16741 cgattatttt tctctctata ttttagttgc actgatttat tgggtcattt gtatcgggat
    16801 cgaaaaagtg caacagtatt tagaaaaacg ttctatgcat cagtcaatct gaaaaataga
    16861 aatggtgcta gcgtggctgg caccatttct attttttgtt tcttgtatat tctgcctagt
    16921 attttaaaac catttcatgt aaaatgtaaa tcgaaaatat gttcggaggt agaaaacatg
    16981 aagaccccgc taaccatcga aatagaatcc tcgttatacc aatattgcat cgagcaaggc
    17041 ggcttggttg tagaagaagt cacgatgcct gatgaccaag gaattgtaga tacgcttagc
    17101 tgcttgacaa aatttgatgg aagccgagaa tggcgatgct atgagctgaa agtgacaaaa
    17161 gcagattttc gctctacagc caaactgtca tttgttggac attataatta ttttgtttta
    17221 ccaagtaaac tatatgaaga agtaaaaact gaaatcccta caggaatagg tgttttagtg
    17281 tatcgtccgt acacggttga agaggagatg cctgcctctg gtacatttct gattgcgaaa
    17341 aaagcagcaa agaaagagct tgctgtaccg gaagaagctt tgacaaaccg cttcatggct
    17401 tctttattca gggaagtaag aaaagcaaag caaatggagc gaggcgtaaa atattttcca
    17461 acagatcaat tgtacaaaga attgaaaaga cgttcagaaa atcaagatcc attttctaac
    17521 gaacgatatt atgaacggtt tttagaggaa atagctagtc aggcaatcaa tgagttgcaa
    17581 gaagaagtta tcgcgcttca gcatgattat gaatatttga ggcaacaaca gcagatccgt
    17641 cggttgccta cagagccatt ggagtgaaaa atatgtacta tccttattta agaggaaaac
    17701 aatttgacct attagcatta aaagaagcac tgtcgcgagg attgctctcc aataaaatcc
    17761 aaccagtgat cgaaccagta cgtgattccg ctacgcttaa aaatgtgatc gaattatttc
    17821 aaaaaaagca ccatcgaatc gctgtgatcc aaaatcccca agtaggacaa ttcaaattat
    17881 tcgatcagca tgtgcacagt tggacagtca aagaaaatag ctctgtcgtc cctgcacaga
    17941 tcttaacgcc agaaaatctc agtgaagtac tggattcacc tccagcgttt cttgttttcg
    18001 atggacagca ctacccaaaa gatacagagg tgtggaaaca gttggcagga gttgattcga
    18061 aatttttgat accagatact agtcgctttc gcatctggct gcccgaaaac aaaattgtga
    18121 tccgtgactc ttttcaaacg agaaagcatg ttgaaagtta tgcagataaa acagatgact
    18181 tttttagtga tgactatctt tttttccacg cagacggata tattggattt tctgatttca
    18241 cgatcgaagg tagccgttat ttcgataaag ggttccctag ccgagcgctt gctatccatc
    18301 taacgtatat tgatgcttac ggcaatatac gcgtgaaaca ttttgtctct gacagtaacg
    18361 actctgctaa agaccaagca ttgaagtttc ttgaagcagg agataaaatg aaaaaatgga
    18421 tcatgcgtca ccatcatcaa ctcttgatta cttcaggaat gattgaatta ttggctcttt
    18481 accagcaaca aaaattccct ggactgggag tgttgaaaaa gtggtcactt atgcatcatt
    18541 tggaacttat tagtcacctg ctagatcatc caaaagattg gcttaaatat tattcaaaag
    18601 ttcaggaact atatgaagtg atccaatgat tagataatgt aaatgaaaaa ggaatagaaa
    18661 agaggaaact ggatgtatat tcggcaaaca aggaaagaag aagtagaaga agtggtagaa
    18721 atcattaatg ccgcagcgga tttcattgct tcgcaaggct ctcctcaatg gcaaaacggg
    18781 cagaaaccaa caaaagaaac gattttgaac gatattgaaa gaaaagaaag ctatgtatta
    18841 atagtggaag atcaaattgc tggaacagct gcattagtct caagcgtgga tcccgtttac
    18901 acaaaaatag atggtagttg gaaagaatca actggtcctt atttatcgat tcatcgtgtc
    18961 gctttagcag aaagtactcg aggaaaaaaa ctagcaaaac cgttcttaga acatttgttg
    19021 acgattgcag ccagcaaaaa tatccaagat atccgtatcg atacttatcc agctaatgaa
    19081 ccgatggaaa agaccatcta ttctgctgga ttcacttatc aagggatgat tgaatttcct
    19141 tttgaacacg gcgaaagaaa agcatatcaa ataatcatca attgactata aaatcatctg
    19201 agcaaagata aagacattaa cactactgaa aaaaacaatc atatgatagc caagaggttg
    19261 taacagaagt ggccagcttc aaaaaacaag aaagaatttc cgaaaatgct tctcaaattt
    19321 ttgtggaatt ttagcgtatt ttacgaaata gctgctttct gaaactccct tcatcgagag
    19381 gtcgtgacat agcttttgtc acgacctctc ggcttatcct acgagtatta ttcgatacat
    19441 ttatatcaat aatccttttt ttattttatc tgcagcttct tggaacgctc catcgtttga
    19501 cacgactaaa agtgagggga tagctttcaa acgtttcaac tgtgctaaat cttctacata
    19561 aaccgccagt ctggctgaaa tctgttcaga tgaatctcca cgttttttca ggcgcgattg
    19621 caatgtttct ttagagatcg taataaagat aggacggact accgtcccga aatactcata
    19681 taaatgccaa aaaccatttg ctgtcaaaac ggcgtaacaa tttcctactg tcagtttctt
    19741 tttaatttct gctttactgg aaccatattt atgttgagca tattggtccc actctgcaaa
    19801 ctcatttccc ttgatcatct gattgaatgt attgtcagaa acaaaataat aatcctcttg
    19861 atctttttcc ccaggtcttg gtgatcgatt cgtgtaagta atgatctttt gttccggctg
    19921 aaaaatagct tctgcgagtg aagtttttcc agaaccagaa ggaccgataa atacatagca
    19981 ataattcatt gtcatttctc ctttgatgat tgaacaagtg aaaaagcttt ggaaaatatt
    20041 tccaaagctt ttaaaagtaa ctttgctttt ttgtatatta taccagcaag ccagtcacaa
    20101 tagcacttaa aaagctgact aaagtcgctg ttagcaaaat tttcaatccg tgttttgcga
    20161 caagatttcc tttttcttca tttaatccct tcatcgcacc agaaatgatc ccgatagaag
    20221 agaagttggc aaatgaaacg aggaagacag atataattgc tgttgtacgc tcagtaaaat
    20281 ggaaattacc atttgctaat tctgtcatag caacaaattc atttgtaacc agtttagtcg
    20341 ccatgatact accggcatcc actgcttctt tccatgggat tccgctgatg aaggcaagag
    20401 gggcaaagat gtaacctagg atctcttgga aagtgatgcc gaatatcccg tggaagatag
    20461 cattgatcat ggcaatcaaa gcaacaaaac cgataagcat agcagcaacc gtgatcgcca
    20521 catggaaacc atcaagaatg tattctccca acatttggaa aaatgtctgt tctttgtttt
    20581 cttcaatgat cagctcatct tctttttctt ctaatacata gggatttaca attgaagcca
    20641 gaatataacc gccaaataag ttcaaaacaa gggcagtgat cacatattta gaatcgatca
    20701 gtgccatata agatccgacg atcgacatag aaactgtaga catagccgat acactcatgg
    20761 tgaacaaacg tttctcagat aggaagggta attccttttt tatggaaata aaaacttccg
    20821 attgtcctaa aatagcagaa gctactccat tataagattc caatttgccc atcccgttga
    20881 ttttacttaa tcctagacca acagctttca taaataatgg aagaatcttg atatagcgta
    20941 gaataccgat aatagcagaa ataaatacaa ttggcatcaa tacggaaaga aagaaaggtg
    21001 cacttccttt atttgctaca ccggcaaaaa caaagtcaac accttgccca gcaaaagcaa
    21061 gaagatgatc aaacattttt gccaatacag aaacaaccgc tgtaccaaaa gttgtacgta
    21121 ataatacgaa accaagaaca agctgtaaaa cgaacatcac aattagtggt ttcaatttga
    21181 ttgctttttt gttactgctg atcaaaaaag caagccctaa tgtgacaaat aaaccaataa
    21241 tacctaataa ataactcaaa taatcactcc tattccaaat tccgttttat tttacctgtt
    21301 ttgcaaataa aatactaatc tttagtgaaa ttttcattac ttttttagag tgaaacgcat
    21361 tcattttcca ttagaaaata aagcgaacaa aataaaaggt ttaaaaagtg taacgtctta
    21421 gtaaagtcta taaaaaaacg aaactatcga ttaagctaat agtttcgttt tcaacattta
    21481 taagtaaact ttttctattg gtaatataga ttttcggaag ggtagacagg tgcgcaggta
    21541 atatcgattc ctagtttttt caatgtttgt tcatcatttt tattgaccat cacagtagag
    21601 tgggcttgtg ctccctcaag ttcagctagt ttatcataag caacttgagc gattggattc
    21661 gttactgcac tgattgataa agcgatcaag acttcattga tgcttagtgc atccaaagag
    21721 gaatgaagtg tctttgattt caatccttgt attgtttcaa gaactaatgg tgacaataaa
    21781 ggaatgtcat ctgcgatatg ggctaaatgt tttactgcat ttaagataac tgccgctgac
    21841 gcatccatca atgaacttgt acgcccggta atgatgactt catctggtaa ttcgattgca
    21901 atcacagcag aaggatcttt tgtaggactt tgcgcttgaa tcttatctgc atagtcatgg
    21961 gcagggacta ccacgtttcg gtcttcagga cgtaaaccga cttcttccat aatcagtttg
    22021 attcgattaa ctgcttcttc gtcgatcaat ccttttttga agtcgcattc tgtttggaaa
    22081 taacgacgga tgatttcttg tttagaggct tcttggacaa cggtatcatc cacgatccca
    22141 aatcctacac gattcacacc catgtcagta ggagattgat aaacagattc tttaccagta
    22201 attttttcaa taatacgttt gattacagga aatgtctcaa catcccgatt atagttcaca
    22261 gctactttat tgtaggcatc aaagtggaag gaatcgatca tattgacatc ttttagatcg
    22321 actgtggctg cttcataagc aatgttcagc ggatgtttca acggaacatt ccatactggg
    22381 aaagtttcga acttggagta accagctgca tgtccttttt ggctttcatg atacagttgg
    22441 ttcaaggaag tagcgagctt tccacttcct gggccaggag ctgtcactac aacgattggt
    22501 ttagttgttt caatatactc attttttcca aaaccatctt ctgagacgat tttttctacg
    22561 ttgactggat agtcttcgat ttctgagtgt ttatagacct tgatgtttcg tctttctagt
    22621 ttattgataa agactttcgt ggcaggctgt ccactatagc gagtgataac gacactgttg
    22681 attaataagc cataaccacg taattcatcg attaaccgca agatatccat atcataagta
    22741 atcccataat caccacggat cttattacgt tcaatatctc cagcatagac gcaaatcagt
    22801 atttcagctt gatctttcaa tttttgcagc aatttgattt tcgcatcttc ttcaaatcct
    22861 ggaagtacac gtttcgcatg cttgtcgtca actagcttcc caccaaattc taaatataat
    22921 ttgtcatagt ggttcactcg ttccaaaata tacgctgact gctcttcaat atatttttct
    22981 gaatcaaaac caattttttt cactaggttc cctccttaag tactaaagca ttttttcatt
    23041 tttttgagga ttcgtcaact ggaagagcga aattcctcaa aaaaacaaag aaaatggttt
    23101 aaaccaactt gtctagatag ttattcattg gtatgatagt ttttgataag gaaggtgcta
    23161 catgaaaaaa agacagaaaa agaaaaacgc atataaacac tatatccgtt cgatttttac
    23221 cggttatgaa agaatgcttg aagatcccga attggaacaa ttgacgttta cttatctaaa
    23281 cgaagaaacg cagctaactc gtgatgagca tcagcgcatt catttcacaa caagagatct
    23341 gcctagtaaa taaaaaataa gataaaggtc atcggattcg attctgtcct atcagatgga
    23401 aagactagtt gatcgaaaaa aaggctgtac caaattttgg tgcagcctgt tttatggagc
    23461 attgcccgct cttttttata acaatttttt acagaaaatg gtactattga agtattaata
    23521 agtgaaaaac agatgagtaa gaggaggcaa gtagtatgaa agagccagat acaaatgcaa
    23581 attatgagcc agacgaacta gaggagacga atgcggatca ggatacagga caagaatcgt
    23641 cttcttttta cgatatcaaa gaaaaagcaa gcatgaatct ttcccaatta ttagaagact
    23701 tacaggcttt tgaaaaagct gtccgcgaag aagatatccc tgagatctac cgtttataca
    23761 atggccgatt gaatgaagaa ttaaaaagaa gctcaaatga aaatcacgag atcgatcaat
    23821 tgctgatgcg taagatccat gatcgttttt tacaggaatt tccttttatg gaacaagtag
    23881 aaaacatctc gccaacgatg agctattaca aaatcggcac ttattaccat gatcggccca
    23941 cggtcggtat tgatgcaagt ctgccagaaa tttttgtttt gcctaagatc gatgaagagt
    24001 gggagaaata ttcccaaccg gagtcaacgg actatgacaa aaaaatcaat gagctagacg
    24061 caaaggtgat cactgctcag acagaaattg aacgtttaga tgaacaaata aaagaaatca
    24121 atcgacaaat gactgatttg aacgacaata aaggattctt gaatcgtaaa aaaatcgagg
    24181 aagaaattca agaattagag aagaagaagc aagtactatc taatgaaaaa ttaggctggc
    24241 ttccttatat tgagacgcct gaaacaatcc agcagcaaaa agaagcattg aagcaagaag
    24301 caagagcaga ccagcttcgt gcagccatcg tagaaaaaga acaaaggcaa atcagacgct
    24361 atttcggtag caaagaggga ttcggtcaag caatccatga attcttgatg aattatcttg
    24421 gcaatgagaa tcctaataat caatctaatg aaggaggttc cgagtatgaa tgaacacgat
    24481 gaagcagaat ggacaaaaaa tgaagaacaa acagaagatc atgaagaaac gaacacttca
    24541 ttggaagaac aaacaagtaa tcctctggat gacacagaag aaatccctat gctagaggaa
    24601 acaaaaatac acgaaaaaga aaaagtaact ccgacgaata cgaatactgc agaaaacacc
    24661 catgcttacg aaataactga aaatcccggg tatttagaag gaaaagagca agatcatcaa
    24721 gatatcgtca gtttgattga aacagcggaa gcagaattag cggatttacg tatgagaaaa
    24781 gatttgatcg aatcagattt tccacaatta ttagaatcat atgatcgttt tatattgaat
    24841 gaagaaccaa tctccacgat cggaaagaca agcctgatcg aatacttcac gtatgaatta
    24901 ctgaaaccgt tgaatgatat tggtctggaa cggtcaaatt cagcttatga cacatggaaa
    24961 acaagacatt tttcgatcaa ttatacgttg aatggacaaa acgaattagt cttttccttt
    25021 gtattaccga ccatcgatca acgataccaa gtggaatcga tgagtctcct aaacgtatca
    25081 ccagagacaa tggaaatcaa tgtccaagat gaccgggtgc tttctttgat ccgacactgg
    25141 tcagtggatc gagtattctc tgcagggcag atcacgatat tcaatcacaa attgaatcaa
    25201 ttattagccc atgcaagaac acttggtttt accgttaatc agacattact agacaatacg
    25261 aagccacttc gtttgaatct tcagagcgaa tttgaattga cgaatgaagt attggatgat
    25321 atttttatcg tggcaatgaa aaatccatcc tacgatatgg aaaagatcaa cgaaactact
    25381 tatcaagtac gattagataa aggacagtct ttaacgataa caacaaacga aaaagatcaa
    25441 acagtcttgt cgatctcatc tggagactac ccacgatctg tcattgattt ctttatcaat
    25501 tacgaattct tggtcccatt aatggtgaga aagtcctgat tggataagtt ggattaccag
    25561 atgtcattca gataattctg ttccaaagga cttgcggcag ttatccagaa taattgtcac
    25621 aagtcttttc tgtatgtact aaatggacgt gaataacttg ctgatgaagg agggatttca
    25681 ttaggaatgt ccacaaataa aatagcacag cttagcttat tgtctgctgc ttgtgtagca
    25741 ggtaggatcg cttttcaatt tattccgaat ttccagccaa tgactgctat ttttttattt
    25801 atagtacttt acttaagctt aaaagatgcg ttaattgtca tgagtctttc tattgcgatc
    25861 agcagttttt attttggtgt tggaccatgg gtcatcggcc aatggatcag ctatactgtt
    25921 gttctgagtt tgttttcaat cgtctgcttg agaaaaacag tgcaaaggaa tctatggtta
    25981 aagggagtct gtttttttct ggcgggcatg gtttacggaa taggtatgac cgtttttgat
    26041 acgctgttat atcgtttgcc tcaaccttgg atctattacc ttcaaggggt gtcgtttgat
    26101 ttgatgcaca gtataggcaa tgttgtgttc tttcttgttt ttctgccagt cgtcaaacgt
    26161 ttttacaatc attcaggagg aaaaaatgaa aaaaataatt tatagtttgc ttgcaatgag
    26221 tgtcagtata ctcattttga caagttgcag tacaacctat acacaatcgg cttccaacca
    26281 aaaggaggaa gtaaaaacat cgcaaaaagc aacaatcacg ttacaagaaa atggtcaaaa
    26341 cttttcggaa aaagaagtgt tcttcaaaaa aggcgatgat ttactgagta ttttgaaacg
    26401 aaattttgaa gtcaaagaag ataacggctt catcacttcg cttgaagggc actcacagga
    26461 tgagaaaaat caactctatt ggatgtttac tgtggacggg aaaagtgcta ctactggtgc
    26521 caaagagatc aaattgaatg atggacaatc ggtcattttc aatttgtcta aactataagt
    26581 ttcataaaaa actgttaaag ggcgaatatt gccataattc agtgagttca gttgatgaaa
    26641 gaaggaaaat aacatggaaa attttaaaaa aagcggaatg ttttccgtaa ttgctgctct
    26701 aggggctaat attctagttg ctatcagcaa atttgtcggc tatgcaatca gtggcagtgc
    26761 cgccatgcta aacgaaagca tccacagtat cgttgattgc agcaatcaga ttttgctgtt
    26821 attcggtgat aaacaagcca ataaaggaca gagcgagttg catcaatttg gtgaaggacg
    26881 agcaaaatac tttttcagta caatcgtcgc aatgatgctt ttcttcggcg gtggggcact
    26941 tggggtgatg gaagcttttg aaaagttgac gcatccatcc catgaagtgg gtaatccgat
    27001 cattgttata gcaatactgt tatttggttt ggtaatcgaa ggaagttcgt tacgcgtggc
    27061 aatgaaagaa atcaaagaat tgaatactga aaaactttcc acgatgcgct ttttgcgcga
    27121 aagtcgacac agtgaaatac tgattatttt tacggaagac ttgtgtgctg taatcggttt
    27181 ggtcttggct ttagcaggaa caatattgac ttatgcaacc gggattgctg catttgatgc
    27241 gatcagcgga ttgctgatcg gtttcttgct gatgggagcc gccattttcc tagcaaaaga
    27301 attttatagt ctgatcatcg gtgaaagcgt gacagcaagc gatttatcaa aaatcaagtt
    27361 agcttttgaa cgtcctgaga tcagtcggct gatcgatata aaaacagttc atttaagtcc
    27421 aacggagatt ttagttgcag caaaagcaga tatcgttggt aacaaagaga atgaagctta
    27481 ctcggtcatc aatgatattg aaaaaaatat gcgtcagtct cttcctgaca agaagatgta
    27541 tatatacatt gaaacagata aatatgaccc aaattattct cgtaaataaa acgtttattc
    27601 atgaattgaa gcaaaaatga gaaaaatata atcttgtttt taaataaaac ggctacaatt
    27661 tagcgaattt atgttacaat gagtccgctg tttatttgtt gtaattaaag gagatatttt
    27721 aatgaacaaa aagaaaagtg cgccatggct tcaagctttg catgcagtga tgccactttg
    27781 tataagctat ataccagtag gattagcttg cggagtattg ttgcaaaaag ttggatttga
    27841 tcctctctta tcaggactgt tatcgattct ggtcttttcc ggtggcgcgc agtttttagt
    27901 tgcatctatg cttacgacgc aagcatcatt tgcaacgaca ttattgatgg ttttcttttt
    27961 agaattacgc tatacattat taggagcaag tcttgccggt tttatgaaaa aagaaaagcg
    28021 gacgtttttg gctgtctttt ctcaatcact gaatgatgaa aactatgcgg tgaactactt
    28081 aaagttttca actgaccctt cctggaataa aagaaaggct ttatatgtca attggttctc
    28141 aatgactgca tgggccggta gcaatatgat cggtaatttt ttcggctcgg tgattcgagt
    28201 agatgctgac cttgtccatt ttgctttgac ggccatgttc attttcatgt ttatcatgca
    28261 gatgaaaagc gcactactga tctttacagg tttattctca ggcgtattag gggtcgtatt
    28321 catggtatta ttccaaaata ctttcggatt gattgcagca acagtcattg cttcatttgc
    28381 cggatttatg ctggagaagg cattcaaaaa agaaaaagct aagaaactaa gagaccgcgg
    28441 taaagatgtc aatgagccgc tcttcaactt cccggaacag gaggccaatc accatgacta
    28501 aactatattt gctactttta gttgcttgtc tgttcatcgt ggcatatatc ccgcgcttgt
    28561 tcccgatgct ttattttact catcgaaaag tgccatcctg gttcagtgac tggatgaaat
    28621 atgtaccggt ggcattattt gcggcgttag cttttaaaga tgttttcatt actcatgaac
    28681 atctggatat tgcttggaat atcaaaatag ctgcaatgat tctagtagca ggcgtggctt
    28741 acaaaacacg ttcgatggcg ttgtcagttt tgacaggact tgcgtctgtc tttttgctgt
    28801 ctatgcttta aactgtttta ttttattaaa aacagagatt gcgacataag ttgttcaaac
    28861 tgatctatcc gaactgcatt tgcaacagtc aattctggat gcaagtttat tcgtagggat
    28921 aaaaaagatt agtgttggct tatgatgcaa gctctttttt attttaggaa ttagagaggg
    28981 atttgtcctt ctatgatttt ttgctataat agggaagatg tttattttat gaaagaatag
    29041 ttgagggaat ttaaatgaat aaaaaattga tgcaaggaac attttggcta acctttgcta
    29101 atcttctatg taaagtttta ggggtagtct atttgattcc ctggctaagt atgatgggga
    29161 acaatcaaga tggtatgctt gctacgacat tgtataatgt tggctatctt ccttatggat
    29221 tgttcctgat gcttggtaca gtcggatttc caaatgcgat tgcaaaaaaa gtagctgtag
    29281 ccacaaaaga aggagataac gctgcttgtc ggctgatttt tagaagtacg atcaatatca
    29341 tgtttgtcat cggtatcgcc tctgctgcac tgatgtattt atttgctcct ttactagccg
    29401 gaatcagtcc gattgctaac gttaataatg ggatactagc tatcagaagt ttatgcccat
    29461 ctttgattgc tatcccaatt cttagtgcaa tgagaggata tttccaagga aaaaatgatc
    29521 tgcggcctta tggaacttct ttgattatcg aacaggctgt gcgagtgatc gttatcttgg
    29581 ctggtactta ttacttgcgc gtattgactg acggaaccat attagaagct gtattgatca
    29641 gtacagtcgc ttctttcttc ggtgggttag ctgcgattat ccatatgtat atcgtcggac
    29701 gcagaaaaga cttcttcgaa cttaaagatt ttttgatttc cagccgttat ttcgaaagag
    29761 aaaaccgatc tgcctctgtc tctatcattc gggaaacact accgtttatt tttgtcggct
    29821 cagtcattac tatcgttcaa atgatcgacc aagtaacgat gaaaccactg cttcactttt
    29881 tccgtccaga gatcgcagat cagcaactgg aattcttatt cagccgtgct tctgtcaatc
    29941 caaataaatt gacattgatc ctgatctcca tggtaggaac agtagctata agcagcttgc
    30001 caatattaag cacgatgaag aaaaaagatc ggatacaaat cgaaaaaaca gtaggagata
    30061 gtttttcgat tgcgttattg attcttcttc cttcattgac tggaatgtct ttattagctg
    30121 gaccactata cacattgttt tttggctatg atcctgaaag tgtcggatat ttccaaatgg
    30181 ctttgctagc ttctttgttt ttctctttat tcacgatcct atctacgatg cttcaatctt
    30241 tgaatcacca tcgttttgcg atccgattaa ctgtagaagc aattatcctg aaagtcatct
    30301 tccaagtaat cggattagga ttagttggcg gatacgggat gagcttatca aatggcttag
    30361 cattcggtat tgtctttgtt cgcggctatc actttatgtg caaagaatac cgaatagcac
    30421 ctttagcaaa gatcagcaat tttttcttaa aaactttccg ctccaccctg attatgcttg
    30481 cagtatg
//
LOCUS       DACEXQ010000012        30154 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.012, whole genome shotgun sequence.
ACCESSION   DACEXQ010000012 DACEXQ010000000
VERSION     DACEXQ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30154
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.012"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..411
                     /locus_tag="IXH04_000392"
     CDS             <1..411
                     /locus_tag="IXH04_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ1851026.1"
                     /translation="GLYYSPVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAP
                     QEYLSRAYNDAPKYCANIVNTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLA
                     ELKGYQVTTGEPVCQTRRLNSRIDKVRYMFNKIT"
     gene            509..697
                     /locus_tag="IXH04_000393"
     CDS             509..697
                     /locus_tag="IXH04_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1851027.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(755..1108)
                     /locus_tag="IXH04_000394"
     CDS             complement(755..1108)
                     /locus_tag="IXH04_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1851028.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            1639..2061
                     /locus_tag="IXH04_000395"
     CDS             1639..2061
                     /locus_tag="IXH04_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ1851029.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            2058..2288
                     /locus_tag="IXH04_000396"
     CDS             2058..2288
                     /locus_tag="IXH04_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1851030.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            2786..2986
                     /locus_tag="IXH04_000397"
     CDS             2786..2986
                     /locus_tag="IXH04_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ1851031.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            3013..4206
                     /locus_tag="IXH04_000398"
     CDS             3013..4206
                     /locus_tag="IXH04_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1851032.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(4274..5839)
                     /gene="guaA"
                     /locus_tag="IXH04_000399"
     CDS             complement(4274..5839)
                     /gene="guaA"
                     /locus_tag="IXH04_000399"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAQ1851033.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(5898..6821)
                     /locus_tag="IXH04_000400"
     CDS             complement(5898..6821)
                     /locus_tag="IXH04_000400"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAQ1851034.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            7017..7622
                     /locus_tag="IXH04_000401"
     CDS             7017..7622
                     /locus_tag="IXH04_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1851035.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            7930..8946
                     /locus_tag="IXH04_000402"
     CDS             7930..8946
                     /locus_tag="IXH04_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1851036.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            9087..10388
                     /locus_tag="IXH04_000403"
     CDS             9087..10388
                     /locus_tag="IXH04_000403"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAQ1851037.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            10434..11096
                     /gene="deoC"
                     /locus_tag="IXH04_000404"
     CDS             10434..11096
                     /gene="deoC"
                     /locus_tag="IXH04_000404"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAQ1851038.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            11117..11515
                     /locus_tag="IXH04_000405"
     CDS             11117..11515
                     /locus_tag="IXH04_000405"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAQ1851039.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            11581..12663
                     /locus_tag="IXH04_000406"
     CDS             11581..12663
                     /locus_tag="IXH04_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1851040.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            12875..14437
                     /locus_tag="IXH04_000407"
     CDS             12875..14437
                     /locus_tag="IXH04_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1851041.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            14430..15563
                     /locus_tag="IXH04_000408"
     CDS             14430..15563
                     /locus_tag="IXH04_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1851042.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            15563..16519
                     /locus_tag="IXH04_000409"
     CDS             15563..16519
                     /locus_tag="IXH04_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1851043.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            16618..17784
                     /gene="deoB"
                     /locus_tag="IXH04_000410"
     CDS             16618..17784
                     /gene="deoB"
                     /locus_tag="IXH04_000410"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAQ1851044.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            17808..18626
                     /locus_tag="IXH04_000411"
     CDS             17808..18626
                     /locus_tag="IXH04_000411"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ1851045.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            18643..19350
                     /gene="deoD"
                     /locus_tag="IXH04_000412"
     CDS             18643..19350
                     /gene="deoD"
                     /locus_tag="IXH04_000412"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ1851046.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(19648..20334)
                     /gene="gpmA"
                     /locus_tag="IXH04_000413"
     CDS             complement(19648..20334)
                     /gene="gpmA"
                     /locus_tag="IXH04_000413"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAQ1851047.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(20506..21087)
                     /locus_tag="IXH04_000414"
     CDS             complement(20506..21087)
                     /locus_tag="IXH04_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1851048.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(21220..21903)
                     /gene="rpiA"
                     /locus_tag="IXH04_000415"
     CDS             complement(21220..21903)
                     /gene="rpiA"
                     /locus_tag="IXH04_000415"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAQ1851049.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            22222..22635
                     /gene="rpsL"
                     /locus_tag="IXH04_000416"
     CDS             22222..22635
                     /gene="rpsL"
                     /locus_tag="IXH04_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAQ1851050.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            22719..23189
                     /gene="rpsG"
                     /locus_tag="IXH04_000417"
     CDS             22719..23189
                     /gene="rpsG"
                     /locus_tag="IXH04_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAQ1851051.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            23283..25370
                     /gene="fusA"
                     /locus_tag="IXH04_000418"
     CDS             23283..25370
                     /gene="fusA"
                     /locus_tag="IXH04_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ1851052.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            25542..26729
                     /gene="tuf"
                     /locus_tag="IXH04_000419"
     CDS             25542..26729
                     /gene="tuf"
                     /locus_tag="IXH04_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ1851053.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            27136..27444
                     /gene="rpsJ"
                     /locus_tag="IXH04_000420"
     CDS             27136..27444
                     /gene="rpsJ"
                     /locus_tag="IXH04_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAQ1851054.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            27475..28104
                     /gene="rplC"
                     /locus_tag="IXH04_000421"
     CDS             27475..28104
                     /gene="rplC"
                     /locus_tag="IXH04_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAQ1851055.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            28132..28755
                     /gene="rplD"
                     /locus_tag="IXH04_000422"
     CDS             28132..28755
                     /gene="rplD"
                     /locus_tag="IXH04_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAQ1851056.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            28755..29045
                     /gene="rplW"
                     /locus_tag="IXH04_000423"
     CDS             28755..29045
                     /gene="rplW"
                     /locus_tag="IXH04_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAQ1851057.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            29084..29917
                     /gene="rplB"
                     /locus_tag="IXH04_000424"
     CDS             29084..29917
                     /gene="rplB"
                     /locus_tag="IXH04_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAQ1851058.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            29960..>30154
                     /gene="rpsS"
                     /locus_tag="IXH04_000425"
     CDS             29960..>30154
                     /gene="rpsS"
                     /locus_tag="IXH04_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAQ1851059.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQED"
BASE COUNT         9603 a         5588 c         6389 g         8574 t
ORIGIN      
        1 ggtttatact atagccctgt tagtactcca gcagattttc ggatgcttac tcctattgta
       61 ctggagcaag cctttagaaa agctggaaca gaattgttag agccatatct tagttttaaa
      121 gtttatgcac cacaggaata tctttcacgg gcatataacg atgctcccaa atattgtgca
      181 aatatcgtaa atactcaact gaaaaataat gaggtcatta ttattggaga aattcctgct
      241 cgatgtattc aagattatcg caatgattta acttttttta caaatgggct tagtgtttgt
      301 ttagcagagc taaaaggata tcaggttacc actggcgaac ctgtttgcca gacccgtcgt
      361 ctaaatagtc ggatagataa agtaagatat atgttcaata aaataactta gtgcgtttta
      421 tgttgttata taaatatggt ttcttattaa ataagatgaa atattcttta atatagattt
      481 gaattaaagt ggaaaggagg agattgttat tataaactac aagtggatat tgtgtcctat
      541 ttgtggaaat aaaacaagac tacgaatacg agtggatact atacttaaaa atttcccttt
      601 atactgcccc aaatgtaaga acgaaacttt aattaatgtt caaaaaatga atataataac
      661 aatcaaagag ccagacgcca agacgcagag ccgataattt gagaaatgaa actctcatct
      721 tatcggctct ttttgtttat ctgaatttta ctgactagcc ttcaatattt cttgcttcat
      781 tgataccttt tgctagtgct tccattaagg atagttcttt gtctgtaaag ctatccatgt
      841 atttttctac ctgtaacctt cgagtgcttt taatcaaatt atctgtaggt aagaaaaatt
      901 catcaataga tacatggagt aatgatacaa gatcatacag cacttgtgta cttgggtgtt
      961 gtcctttgtt ttctatattt gttaaataac gtgggtcaat ttcaatcatt gttcccactt
     1021 gttcacgagt gagaccccgt ttcattcgag cctctttaat cgctaaacca acggctctaa
     1081 aatcgtattt atcttctttt ttacgcatac taaatcacct ctatacattt tactgttcct
     1141 atataaccat aaacaggtat agaaaaacat gttatatagt ttatgagttc ctatagaaca
     1201 ctgtggaatt tattaatgcg atgaatccaa tttcaaatag aaaggtcgct gattttattt
     1261 acctatacaa ctttataaaa tttagtcata ctcctagaaa gtagataaaa ttggcgactt
     1321 aatggaggga aaagtcgcca tgagaaagat aatctgtcat ctagttctct taattaagtt
     1381 cttatcaaaa aaacttatcc acctgtactg gatagatatg accatcaatg tggttttgca
     1441 gattacatag atagaaaaat caaagcatat gaacagagaa aacaatctgt ttatgtgctt
     1501 ttttggtcac ttaataaata tcttttagga tttctcttca tttatgcaac aaatcccccg
     1561 ttcgtgtcgg ggtttagtat gaagggagat aaagaagcac atgccactcc ctttcacgac
     1621 agaaaggggg tgaaaagcat gaaaccatct tcttttcaag aaaccataga aaatcagttt
     1681 gactatatct gcaaacaagt tattgaagat gaacgaaaag attacttcaa acatctagac
     1741 agactatcag caaaagaagt ctcgttttct gatatagata actaccttgt caactctttt
     1801 tctactgttg atacgtacgt cactgatttc caactattta aattatttgg attaagaatc
     1861 aatgttgaaa gtgatttatt aagtgaagcc ctgcgtcatc tttcagagaa aaaacggaac
     1921 attattttac ttcattactt tatggatatg agtgatgtgg agattgcgga gttactaaat
     1981 ttgaatcgtt cgactgttta tcgtcatcga ataagtggac tagccatgat taaagaattc
     2041 atgaaggagt gtaaagaatg aaaacagctt atccaagggt tcccttcccc ctcattgtaa
     2101 aggcgacaga tggcgacgtg gaagcaatta atcagattgt taaacattat cgagggtata
     2161 cctcaaaacg ttcattacgt cgcatgacag acgaatatgg aaattctcat atggttattg
     2221 atgaaaccct acgtggcaga atggaaacga aactcatctc taagatttta tcgttcgaaa
     2281 ttaaatagtc ccattttttc gtggaagcgt gtcgaatcgt atccacgctt cccgatttgg
     2341 gaggtttgtt attccatcaa agcatattgt gtgttcagta ggttttgata ggctaacaag
     2401 ccaaacgttc tttgacaacc aaataaaggc aatcgaatac gtttcggtaa gaaaagagct
     2461 gatgaactaa cacgccatga cctacaaaat tagtgagcga ttcatgttag taattcgaaa
     2521 gtaatcttta gcaggattgc tgccatgact ttcttatcgt gataatgata ctcccgtata
     2581 gccatagtcc gagcgttaga agcgtcacag gcaatgggta cggctacatg agaaccatgc
     2641 agaagtgaaa ttcctgtgag ctttgctaga gctgttcgat tgctaagata ttcttctctt
     2701 gtcagtgtat tattataaaa ctatttgata taattatagg tgaatattgg agttttccta
     2761 gcctataatt gggaaaggag taataatgat aacggtcaca aaaaaggatt tagttgagct
     2821 aggatatgga acttcttttt cagcagacat cataaaaaaa gcaaaagaat tgatgattac
     2881 aaaagggcat atctactatc aatctcgaaa gctagataga gtccctcgtg aagctgttga
     2941 agaaattcta ggaattcggt tttcaaatga aaacaggtct gagtagtttt tgcacagtaa
     3001 ggagtaaaaa ctatggttaa agacccaata aaaaaagcaa agaatggaac ctattatttt
     3061 cgtgcaaatc taggttatga ttctaaaggg aaaaaaattc aaaaatatcg tagtggattc
     3121 actactaaaa aagaagcaag agaagaatat tcgaaactgc tattgatgaa accagaagaa
     3181 ttaagtgaga ataaggataa aatgacgttt aaacattata tctttgaaat tttccttcca
     3241 tggtataaga cacaggtaaa gctcagaaca tatgaaaatc gcttaccaac tattaagaag
     3301 catttctctt attttgataa aatggctgtt tccgatattg agcctattga tgtgcaaaat
     3361 tggcaactaa aattatcaaa gaagtgtaag tcttcttatg taagagctgt tcaaggacta
     3421 ttttcagttg ctatggatcg agcaattgtc cttgggataa ccaccacaaa tccatctaag
     3481 attattggca atgtgaaaaa gcaaaagtct aaaattgaat tttggacaaa agaagaattt
     3541 gaaaaagtaa tctctctaat ttacaaagaa gattactatc aacacttttt atttatctct
     3601 ctgtggtttt tatttatgac tggaatgcga attggtgaag ctactgccat tcaatgggaa
     3661 gatattgatt ttgactcagg agtactaacc atcaacaaaa cactctatta caagaatcaa
     3721 tcgaattata gatttgttga accaaaaaca aaggcgagcg ttcgtcatat tgtattagat
     3781 aaatgcacat taacttattt atccgaatgg aaggaagtac aacaaaatct aattcagaca
     3841 gactttgtaa tgagctataa cggtattcct acccaaaaac atacactatc atatgctatt
     3901 gaacgctatg ctaagctggc tggtatccat cgaatcagaa tccatgggtt acggcattct
     3961 catgcttcct tgttaatcag tatgggagaa aatccactta tcattaaaga tcgtctagga
     4021 cacgaagata ttgagacgac tttaggaacg tatggacatc tttatcctaa cagtaatttc
     4081 gaggtagcta acaaattaaa tggagccatt tcctttaaag aagcaaaaca aaattatgat
     4141 acttcaccaa agaatcaatt tactgtagac tacttgcgaa acaaagaaat gaaaaaagtg
     4201 caataaaagt gcagtaaaaa gataagaaga gccagaaacc cttggtataa gaggatttct
     4261 agctctccta caactattcc cactccactg ttgctggtgg tttagaagtg atgtcataca
     4321 cgatacggtt gacgtgggct acttcgttta cgatacgtac agagattttt tgcaagactt
     4381 cccatgggat acgggcaaag tcagcggtca ttccgtcgat tgaagttaca gcacggatac
     4441 cgactgtgta gtcgtaagta cgtccgtcac ccataacacc gacagagcgg atccctggaa
     4501 gaacagtgaa atattgccag atatcacggt caagtccagc agctgcgatt tcttcacgca
     4561 agatagcatc agattcgcgt acgatttcta gcttgtcttc agtgatttcg ccaagaacac
     4621 gaataccaag accaggacca gggaatggct gacgccaaac gattgaatca ggcatgccta
     4681 actgagtccc taattcacgg acttcgtctt tgaataatgt gtttaatggt tcgatcaatt
     4741 caaattgcat atcttctggc agtccaccta cattatggtg agatttgatc gtctgtgctg
     4801 tttctgtacc agattcgata acgtcagtat acaacgttcc ttgtgctagg aaagagacgc
     4861 cttcttcacc agcaagtttt gttgcttcgt catcaaaaac atatacgaat tcattcccaa
     4921 tgattttacg tttttgttct gggtcagata cacctttcaa cttgtctaag aagcgatctc
     4981 ttgcatcgac tttgatgatg ttcaagccga attttccgcc tagactttcc attacttgtt
     5041 ccgcttcgcc tttgcgtagc agaccgtggt caacaaagat agatgtcaat tggtcaccga
     5101 tcgctttttg taaaagaacc ccaacgacac tagaatcaac gccacctgaa agaccaagca
     5161 agactttttt gtcaccaact tgttcacgga ttttttctac ttgcatatcg ataaagttat
     5221 ccattgtcca gttaccttca cagtgacaaa catcaaaggc aaagtggcgc agcaattcga
     5281 ttccgtattc ggaatgacgt acttctgcat ggaattggat accataaaaa ttacgtgttg
     5341 tgtcttggat agaagcaatc ggacaatctt tgcttgttcc gactgtttca aagccttcag
     5401 gaactttagt taccaagtca ccatggctca tccatactgt ttgttgtttt ggtgtttctt
     5461 taaacaagac agcttgatcg cttgttactt ctaacaaggc tttcccatat tcgcggtttt
     5521 tagcaggctc tacggaaccg ccaagtttgt acgtgatcaa ttgcatacca tagcaaatcc
     5581 ctaatacagg aatacctaaa ttgaagatct caggatcgat cccaaatgca ttctcatcat
     5641 atacactgtt tggtccgcca gaaagaatga ttccctttgg attcatcgct tttacttctt
     5701 ctgcagtaat gcggtggctc aataattcgg aaaagactcc aagatcacga atacgtcttg
     5761 tgatcaattg gttatactga ctaccgtagt ctaatacgat aatcttttca acgttcgtca
     5821 tatcggcaac gtttgtcaaa tttattaccc ctatcccttt aaattttatc tcaaaatagt
     5881 caaactattt taaaatgcta atattttcgc atgaaaatcg aatcgatgat atgattttca
     5941 gttttatgga gaattatatc ggctcttcct cgtgtaggca agatatattc ctgtaaattt
     6001 ttcaaattca catttttcca tacatctttt gccattttaa aggcttcttc acgatctccg
     6061 atagcaaaac gataataata attctctgga ttttggaaag ccgtatccag taacgcacca
     6121 aaacgatcca aataccattt ttcgatcagc acgggatcgg catcgacata gacagaaaaa
     6181 tcaaaaaagt cgctgacgta gatttgctga ttcgcaggta attgaagcgt attgatcccc
     6241 tcaacgatca agatatcagg ctgctggatc aattcgtact tgtctggaat gatatcatac
     6301 acatcatgtg agtaaacagg tacttttatc tcttcctgac cagattttac acggttcaag
     6361 aaatcgatca gcatctccat atcataactt tcaggaaatc ctttacggtc catgattcca
     6421 cgctctttta atatacgatt tggataaaga aatccgtcag tcgtgatcag ctgaacgttt
     6481 cttcgtttga aggtacgaga caaaatcgtc tgcagcagtc tggctgttgt actttttcca
     6541 acggctacac ttccggcgat cccgataata aaaggcggta cttctacgta tttatgaaga
     6601 aataaacctt tactcaagaa caaagattca aattctttca tataaagata aatcagatga
     6661 gcaagaggta catagatatc tcgaacatcc tgcatggaaa tgcggtcatt caaacttttg
     6721 atattatcta gttcatcttg tgtcaaaggt gcttgcccat tacgataaaa ttctcgccat
     6781 tcttctcttg ccacacggta gtagttcatt ggatcattca tattagcctc ctgcgttctc
     6841 ccatcaatgg aactatttta acacaaaata aacgcaaacc ccaccactta agagagattg
     6901 ccgatctttt tgtataatct ttataataga aagaaacgga gtcttattct tgacttgcgc
     6961 acggatacat aaaaggatag actgttgtca tgaaagaaat gagaaggatg gaagacatga
     7021 caaatcatta ctattctgaa cgcccagata cggcgcacga ttttgaacaa tgggcttttg
     7081 agttaaaagg cagaaccttt caatttgtaa cagattctgg tgtattttca cgtgaaacag
     7141 tggattatgg ttcacgtgtc ctgattgatg ctttcgattg ggaagagtta cctgatgaag
     7201 gaaagatctt agatgtggga tgcgggtatg gaccgattgg gttagctttg gcatttgcga
     7261 cacagcggtt tgttgaaatg gtcgacatta atgaacgagc ggtaagtcta gcgcaaggaa
     7321 acgcaaagcg taaccaaatc gaacatgttg atatccatca atcgaatatc tatgaagcag
     7381 ttcatgaaga aacctatgca gctatcgtaa gcaatccgcc gatccgtgct ggaaaaaaag
     7441 ttgtacatga aattttgaca ggcgcatatc ctcggttgaa gaagggcggt acactgacaa
     7501 ttgtgatcca gaagaaacaa ggagcgccga gtgcgcaaaa gaaaatggaa gaaacatttg
     7561 gtaatgcaga aatcgtcaca aaagataaag gatattatat catcaaaagt gtgaaagaat
     7621 aactagctga aaaatagtgg agcgacgaaa gagtggaact gggcgtgcat gtacgggtac
     7681 gtatcagttc tttttttgtt gagaagtttt taaaaacaaa gaactatttt ttcttcaaaa
     7741 agaaatagta ggaacagagt cgttgttttt tacgataaaa taaataagat acagaacttg
     7801 tatagaaggc tgccaagtcg gtagataaag gaggttttta tcaaaaaaca tactttcctt
     7861 ttttctctat tcttttcttg ggggttttgg tacactgata gggaatttag atagaaggga
     7921 gccaatagga tgaagctgac catcagagat atcgcagaaa tggcaggcgt atcagtaaca
     7981 actgtctcac agattttgaa taacaaagga agcagattta gtgataagac aagaaaaaaa
     8041 gtgttgtcga tcgtgaatga acatcattat aagcccgatt atttcgcttc aaatgtgatc
     8101 aatcgacatt caaagaccat cggaatgatc gtccctgatg tcactgattt cttcttctcc
     8161 aaactgatcg aaggtgtaga gagctatttg aatcctttgg gctatgtgat catgttatgc
     8221 aattctcgtc atagccaaga aaatgagatc aagtatttac aagagctttc ccaccgctca
     8281 gtggacggaa tcttgctggc cacaccaaat attttacctg agaaatatgc attagacagc
     8341 ggtttttatc aaaaaatgcc aatcatttta atcgatcgcg gattgaatcg tcgggataac
     8401 ggacggctta tcgtaaagga atatgagggc gcctatcaag ctgtttcgct gtttattgaa
     8461 aatggccata cgaagatcgg catgctgaag gaaaccactg gatactatca attagaagaa
     8521 cgtttcaacg gatatcgtca tgcgctgaaa gatcatggcc tgccttttaa tgggaaattt
     8581 gttgaacatg gagagttgac tgtccaaggt ggatatactg catcaaaaaa attactcaaa
     8641 cataaagata tcacggctat tttttgcggg aatgatgcca tggcgatcgg atgctatcag
     8701 gcgatcgatg aattgggcaa aaaaatccca gaagatatct cagtcatagg attcgatgga
     8761 ttaaagctgt cagaatacat gattccgcaa ttgacgacag tgcagcagcc aagttttgat
     8821 attggatttt atgctgcacg tttcctgatc gatacgatcg aatttcctca aagaaaagtt
     8881 ccgaacaaag tgttcgaaac aaaattaatt attcgtgaga gcgtaaaagt attgacaaag
     8941 ggctaactcg gcggtatatt gcttatgtaa gcatttacaa aggctagtta gtgctttttt
     9001 tatttttagt aaaccagttt actaaagtgg tttacctttc ctaaaaataa tgtataatac
     9061 cgaatgaaga caaggaggat caggcaatgc gaatggtgga tctgattgaa aaaaagaggg
     9121 atggaaacga gctgtcgaaa gaggaaatcg agtacatcgt cacgaactat acgaatggaa
     9181 agattccaga ttatcaagtc agtgcattat tgatggcaat tttttatcaa gacatgacaa
     9241 atgaagaaat cacgaactta acattggcga ttgctaattc tggtgatgtc atcgatttga
     9301 gttctcttga aggaataaag gtagataagc attcgacagg tggcgttggt gacacgacca
     9361 cattgatttt agccccccta gtagctagtg tcggagtgac agtggctaaa atgtctggac
     9421 gcggtctagg ttacacaggt ggtacactag acaagctaga agcaattccc ggatttcaga
     9481 tcgagctttc ggatgaagaa tttgtacgca ttgttaatga aagtaaagta gcggtaattg
     9541 gtcaatcagg aaacttggca ccggcggaca aaaaattgta tgccttgcgt gacgtcacag
     9601 cgaccgttga ttctcttccg ctgattgcca gttcgattat gagtaaaaaa atcgcggcag
     9661 gagcagatgc aatcgttttg gatgtgacca ctggagacgg tgctttcatg aagaacatcg
     9721 aagatgcaag acgtctggca aagaccatga cgagcattgg gaaactggct aaccgagaaa
     9781 cagtggcggt tatctcagat atgtcagaac ctcttggcga agctatcgga aatagtctag
     9841 aagttgtcga ggcaatcgaa acattacaag gaaatggtcc agaagatcta gtagaaatgt
     9901 gctacgcctt aggaagccaa atggttgttc ttgctggcaa agctaagaca accgatgaag
     9961 caagaacgtt gcttcaagaa gctctggaat caggtaaagc gttagcaaaa ttcaaagaaa
    10021 tgatccaaaa tcaaggggga gacccagcga tcgtggaaca tccagaacga attttgactg
    10081 cacgctacac aatggagctt cctgctaaac aaagcggagt tgtgtcaaaa atcgtggcaa
    10141 atgaattagg aattgctgca atgatgctag gtgctgggcg taaaaccaaa gaagaagata
    10201 ttgatcacgc tgttggattg aaactgcata aaaaaattgg tgacactgtg acaaaaggtg
    10261 agtcactatt gacgatctat agtaacgacg aagaaatcac ttctgtcatt gaattattat
    10321 ataaaaatat tgaaattggt gaatcggcaa tgaagccaac gttgattcat gacatcatca
    10381 cagaataaag gaaaaaagcg gactttaccg ttttaaagaa aaggggaaaa gaaatggaac
    10441 taaaccgaat gatcgaccat actattttga aagcagacgc atctaaagaa gctgtgatgc
    10501 aaattatcga agaagcaaaa aaataccatt tttattctgt ctgcatcaat ccagcatggg
    10561 ttgcgctagc gaaagaacaa ttacaaggtg agccagtagc cgtatgtaca gtaatcgggt
    10621 ttcctctagg agcaaacaca cccgagacaa aagcatttga aacaacagat gccatcaata
    10681 acggtgcaga tgaagtcgat atggtcatca atatcggtgc tttgaaatca aaggatgatc
    10741 aacaagtcca aaaagatatc gaagcagttg ttgaagcagc aaaagatcgt gcgcttgtca
    10801 aagttattat tgaaacatcg ttattagata gagaagaaat catccgagct tgcgaaatag
    10861 caaaagctgc aggcgctgat tttgtcaaaa catcaactgg tttctcaaca ggcggagcaa
    10921 aggtagagga tgtccgtttg atgcgtgaaa ctgtaggacc agaaatgggt gtcaaagctt
    10981 ccggcggtat ccataacgaa gaagaagcaa tggcaatgat cgaagctggt gctacaagaa
    11041 tcggaacaag cgcaggagta gcaatcgtat ctggttcaac tggtgaaggc tattaatttt
    11101 ttcaaaggag aagctcatgg cagaactcaa acaagaatgg atcaacacag ctattgaagc
    11161 gttagataaa gcctatgttc cttattccca ttttccggta ggcgcttgtc tcgtaacgga
    11221 atcaggcaag atttatcaag gcatcaacat tgagaatgca tcatttggat taacgaattg
    11281 tgccgagcgc acagcttttt tcaaagcagt atctgaagga gaaaaaacat ttacgcatct
    11341 agtcgtagcg gggcacaccc ccgatcctat ctctccttgt ggtgcatgtc gacaagtaat
    11401 ggcagaattc tgttcccctg atatgccggt tacgctagta ggagacaatg gtgtgacgaa
    11461 ggcaacgacc gttcgtgcac tgctgcctta tgcatttact gaaaaagatc tataactcat
    11521 gttcattcag tcagctgact gttgaaaaat aaaacaatta ttttaggagg ctttacagaa
    11581 atgaaaaaag caaaattatt tggtttaggt gctgtcgcac tagcagcagg gttattttta
    11641 ggtgcatgcg gcaacaacgg ctctactgat tcatccggtg gcaaaagctc atcagacaca
    11701 acaactgcag cattgatcac agacacaggt ggtgtagacg accgttcatt caaccagtca
    11761 gcttgggaag gactagaaaa atgggggaaa gatcaaggac tttcacgagg aaatgatggc
    11821 ttccaatatt tccaatcttc aaatgaatca gattacatcc caaatattga ccaagcattg
    11881 aatgctggat tcaaaacgat ttttggtatt ggttataaac taaaaccagc gatccaagaa
    11941 caagcaacaa acaatacagg tacgaacttt gttattatcg atgatgtcat cgatggatta
    12001 gataacgttg tatctgcaac tttcaaagac aacgaagctt cttatcttgc aggtgttgca
    12061 gcagcttata cgactgaaac aaacgtagtc ggctttatcg gcggtgtaaa aggtgaagta
    12121 atcgatcgtt ttgatgctgg attcaaagcc ggtgttgatg caggtgcgaa agaattagga
    12181 aaagaaatca aagtattgaa tcaatatgct ggtgacttct ctgctccaga taaaggacgt
    12241 tcaattgctc aaggtatgta tgcacaaaac gcagatatca tcttccatgc ttcaggcggt
    12301 acaggtaacg gtgtattcca agaagcaaaa tcattgaacg aatcaggcga caaaaaagtt
    12361 tgggttatcg gtgttgaccg cgaccaatca gacgaaggcg aatacacatt aaacggcgaa
    12421 aagaaaaact tcacattgac atctacactg aaagcagttg gaacagtagt agaagatttg
    12481 gcacaaaaat cagcggacgg taaattccct ggcggcgaac acactgttta tggattgaaa
    12541 gaagacggtg ttggtctaac agaaggacaa ctttctgacg aagcgaaaaa agcagttgat
    12601 gaagcaaaag aaaaaatcat ttcaggtgac gtaaaagttc cagaaactcc tgaagaaaac
    12661 taaaaccatt tctatagcct tgttagacac atgtttgaca aggctatggt ttgattagaa
    12721 caagaattgt tcatttatcc aagctgcctt gtgacagttt ggataaggga acaactccct
    12781 gaagtaatgt ttgtttcaaa agaagaacgc tcactaagcg tgaatcttag tcaatcgttg
    12841 ccctagatag aaatcgttag gatgtgaaaa aaaggtggtt caagaaaata ttgtcataga
    12901 aatgcagaat attaccaaag aatttggaac attcaaggca aatgacaaca tcaatttaca
    12961 agtaaaggca ggagaaattc atgcgttgct tggagaaaac ggtgctggca aatctacatt
    13021 gatgaacgtg ctttccggat tattagagcc gacatcaggg aaaattttga tgcgtgggaa
    13081 agaagtaaag atcacaagtc cgaccaaagc caaccaatta gggattggga tggtccatca
    13141 gcactttatg cttgttgatg cctttactgt aacagaaaac atcgtgttgg gaagcgagcc
    13201 tagtcgcgca gggatgcttg accataaaaa agcacgaaaa gagatccaaa aagtttctga
    13261 acagtatgga ttgtcagtca acccagatgc ttacgttcgt gatatttcag ttggaatgga
    13321 acaacgggta gaaattttaa aaacacttta ccgaggagca gatgtactga tttttgatga
    13381 gccgacagct gtattgaccc ctcaagaaat tgatgaatta atcgtgatca tgaaggaatt
    13441 agtcaaagaa ggcaagtcga tcattttgat tacgcataag ttagatgaaa tcaaagcagt
    13501 agctgaccgc tgtacagtta tccgccgtgg aaaaggaatc ggtacagtca acgttaaaga
    13561 cgtaacctca caacaattag ccgatatgat ggttggaaga acggtttcat tcaaaacgat
    13621 gaaaaaagaa gcgaagcctc aagaagtcgt tttgtctatt gaaaatctag tggtgaaaga
    13681 aaatcgtgga ttagaagccg tgaaaaactt gaacttagag gttcgtgctg gcgaagtact
    13741 tggtatcgct ggaatcgatg gaaacgggca gtcggagttg atccaagctt tgactggttt
    13801 gcgaaaggca gaaagcggac atatcaagct aaaaggggaa gacatcacta ataaaaaacc
    13861 ccgaaaaatc actgaacatg gtgtaggaca cgtgccagaa gaccgtcata aatacgggtt
    13921 agtcctagat atgacattat ctgaaaatat cgcactacaa acctatcatc aaaaacctta
    13981 cagtaaaaac ggcatgctga attattcagt aatgaatgaa catgccagag aattgatcga
    14041 agaatatgat gttcgaacaa cgaatgaact tgttcccgca aaagctttat caggcggaaa
    14101 tcagcaaaaa gcaattatcg ctcgtgaagt cgaccgagat cctgatctgt tgattgttgc
    14161 aaatccaact cgtggactgg atgtaggtgc gatcgaattt attcataaac gtctgatcga
    14221 acaaagggac aaatacaaag cagtgttact gatcagtttc gaattagaag aaattttaaa
    14281 tgtttcggat cgtattgctg ttatccatga aggagaaatt gtcggtatcg ttgatccgaa
    14341 agaaacatct gaaaatgaat taggtctatt gatggcgggc tataccttag aagaagcacg
    14401 aaaagagtta gaaaaggggg ctggcgcaca tgaatgatcg cagcgaaaaa ttccgtaatt
    14461 tattagtgcc tgtactttca gttgttttag gattgatctt aggcgcgata ttgatggctg
    14521 cttttggttt tcagcccatc caaggttatt cttctatgct agatgcagcc cttggttcgc
    14581 aaagagggat aggtgaaacg ttaaggcaag caacgccgtt gatttttaca gcattaggtt
    14641 tttctgtagc taactcagct gggtttttca atattgggct ttctggacaa gcactttgcg
    14701 ggtggatcgt cagtgtctgg gtggcacttg cgtttccaga tctgccaaaa gttgttttgc
    14761 ttccgttatg cgtgatctta ggtgccttag ctggtgcgat ggcagcggct gttccaggtt
    14821 tacttcgagc attttttgga acaagtgaag tgatcgtaac aatcatgatg aactatatct
    14881 tattatacgt aagcacattt gctttacatg aagtgatgcc tgaaagctat cgctcaagtt
    14941 tggactcttc caatatgatc agtcaaaatg cctcactgcg gttaccatgg ttaacacaag
    15001 cttttggcgg ctctcgcgta aactcaggat tatttttagc aatcatcgcg cttattttcg
    15061 tttggctgct gatgaagaaa acgactttag gatttgaaat ccgttctgtt ggattgaatc
    15121 cttttgcttc tgagtatgca ggaatgagta gtaaacgtac gatcgttttg tcgatggtca
    15181 tttctggcgc tttggcaggg cttggcggtg tggttgaagg attaggaacg tatcaaaact
    15241 tctttgttca aacgacttcg ctatctattg gatttgacgg aatggctgtt tccttgcttg
    15301 gttctggatc agcaatcggt attcttttat cagcgatctt gttcagtatt ttgaagatcg
    15361 gtggacttgg catgcagaca ggagcaggtg ttccgtttga gatcgtcaat gtatcgattg
    15421 ctttgattat cttctttgtc gggatcaact tcctgattcg tttcgtcatg gctaaagtct
    15481 tccaagggaa aaaacaagaa gagatcgttg cgacaataga atcgaaacca acgaatcaaa
    15541 accatgaagg gggcgaacta taatgtcgac tatcgaatta attgccttga tccttacttc
    15601 tacactggtt tattcaacac cgctaattct aacgtcacta ggcggaactt tttctgaacg
    15661 aagcgggatc gtaaacgtag ggctagaggg aatcatggtc atgggtgctt ttagttccat
    15721 cgttttcaac ttgacgatga gtccatcact tggttctatg acaccatgga tcgctatttt
    15781 agtgggtggc gtcgtaggta tcctattttc tctgattcac gcagtagcaa cagtcagttt
    15841 gcgtgctgac catattatca gcgggacagt aattaatctg atggcacctg ctttaggtgt
    15901 tttcttgatc aaagcatggt atggtaaagg tcaaactgat aacatcacgc agaatctggg
    15961 gtatttttct ttcccaggat tagcagatat tccggtcatt ggaccaattt tctttaaaaa
    16021 tacattctta cctgcttatt gtgctatttt gattgctgtc ctagcttggg tgatcatctt
    16081 caagacgaaa ttcggcttgc gcctccgttc agttggtgaa aatcctcaag ctgccgacac
    16141 attaggtatc aatgtatatg ctatgcgtta ttcaggcgta ctgatttctg gcttgttagg
    16201 cggtatgggc ggtgctgttt ttgctcagtc gatttccggt aacttttcag ctggtacgat
    16261 cgttggacaa gggttcatct ccatggctgc tatgatcttt ggtaagtgga atcctttagg
    16321 ggcaatgggt gcctcattgt tctttggttt tgctcaaagc ttgagtatca ttggcgcaca
    16381 attaccagtt atttcttcga ttccgccagt attgctgcaa attgctccat atttgctgac
    16441 aatcattgtg ctggttgttt tcttagggaa agcttctggt ccaaaagcaa acgggaagaa
    16501 ctatatcaaa tcaaaataat cttttagcga ataaaaaggg tgggttacgg gaagtgtttt
    16561 tcgtctcgcc ctttttgttt taaaggcaat aaaaataaaa agaaagaagg aataatcatg
    16621 tttaaacgcg tacatctgat cgtcatggat tcagtaggaa ttggagaagc accagatgcc
    16681 gataagttcg gtgatgttgg ttctgataca ttaggacaca ttgcgaaaga agcaggattg
    16741 aacgttccac atttagagca gcttggttta ggaacgatcc gtcctcttga aggtgtggaa
    16801 tcagtgaaag atcatcaagg ctatgcgacg aaattagaag aagtttctgt agggaaagat
    16861 acgatgacag gccactggga aatcatggga ttgaatatca aaactccatt tcgtgtgttt
    16921 cctaatggat tccctgatga tctgatcaaa caaatcgaag aattttctgg ccgtaaagtc
    16981 gtatgtaaca aaccttatag cggaacagct gttattgacg attacggtga acaccaaatg
    17041 aaaacaggcg atttaattgt atatacttct gccgatcctg tccttcagat cgcggcacat
    17101 gaagaaatca ttcctttaga agaattatat cggatttgcc aattcgttcg tgatataaca
    17161 aaagatgatc cgtatatgat tggccgaatc attgcacgtc cgtacgtggg tgaaccagga
    17221 gacttcacac gaacaagcaa tcgccatgat tatgcgttga atccattcgg acacacagtc
    17281 cttgattctt tgaaagaagc aggaaaggac gtgattgctg ttggaaagat caacgatatt
    17341 ttcaacggac aaggaatcac tgaatctgtc cgcacgaaaa gcaatatgga tggcgttgac
    17401 cagctgctga aagttatgaa aaacgatttt tcaggaatca gctttaccaa tttagtagat
    17461 tttgatgcat tatacggtca tcgtcgggat gtagtcggct atgcgcatgc gattgaagaa
    17521 tttgacttac gaattccaga attgcttgaa gcgatggaag aagacgactt gctaatgatt
    17581 acagccgatc acggaaacga tccgacgttt actggtaccg atcatacgcg agaatatgta
    17641 ccgttattgg tttacagtaa aaaaatgaaa gaacaagggg cattgcctca aggatattat
    17701 tcagatattt cagcaacgat tgctgagaac tttagtgttt cttcgactga aaatggcgaa
    17761 agcttcttga aattattaaa ataatctttt taaataggag aaagcaaatg accaagttaa
    17821 gcgagatgtt acaagaaacg acccagttta tccttgataa aggagtaaaa gaaattgatt
    17881 ttggtttgat tctaggttca ggattaggtg aattagcaga tgaaatcgaa gatgctgtaa
    17941 tgatttctta tgatgagatc ccgttctttc ctgtttcgac agtagttggg catgcaggaa
    18001 agctagttta tgggtctctg gcaggcaaga aagtgttagc catgcaaggg agattccact
    18061 actatgaagg tcattccatg caaacagtga cctatccagt acgtgtgatg gccggactag
    18121 gggcccattc agtcattgtg actaatgcat gtggtggtgt caacgaatca tttgtaccag
    18181 gagatttgat gctgattacc gatcatatca attttactgg tgaaaatcct ttgatcggtc
    18241 cgaatgaaga tgcaatgggc cctagatttc cagatatgag tcaagcttat acgcctgact
    18301 atcaagcaac agcaaaagca gcagcagaaa aactaggttt ggatcttaaa gaaggcgtat
    18361 atatgggcta ttctggacca acttatgaaa cgcctgcgga gatccgaatg tctcggacaa
    18421 taggagccga tgcagtaggg atgtcgactg ttccagaagt catcgtagca gctcacagcg
    18481 gattaaaagt gttaggaatc tcttgtatta ccaacttagc agcaggtatg caagcgaatc
    18541 tgagccatga agaagttgta gaaacaactc agagagtcaa acaatctttt aaagcattgg
    18601 ttaaagaaac attgacatta ttgtaatagg aggaacaata aaatgagtgt gcatattgaa
    18661 gcgaaagaag gcgagatcgc cgataagatt ttacttccgg gagatccttt gcgagctaaa
    18721 tatattgcgg agactttttt ggaagatcca gtttgttaca atcaggtacg cggtatgtta
    18781 ggctttaccg gcaaatataa aggagaacgt atttctgttc aaggaacagg aatgggaatg
    18841 ccatcagcca ccatctatgc ccatgaattg atccagtcgt atggtgtaaa gaaattgatt
    18901 cgagtaggta catgcggtgc attgtcaaaa gacgtccatg ttcgtgattt agtactagca
    18961 caaggtgcag cgaccagttc ttcgatgatc gaaaaaaatt tccaagcctt tcattttcct
    19021 ccaatcagtg atttcaatct attactgaaa gcatatgaga tagctaaaga aaagggctat
    19081 acggttcatg taggaaatgt tctttcggaa gattctttct acaaagatga tttgacagag
    19141 acgttccaat tagctgaact tggcgtttta ggagtggaaa tggaagcagc ggctctttat
    19201 tacttaggcg cgaaatacca tgtgcagaca ttaagtttga tgaccgttag cgaccatttg
    19261 atcaccggtg aagaaacaac agctgcagaa cgtcaatcta cgttcaatga aatgatcgaa
    19321 gtaggattgg aaacagcgat agcagaataa gaaggaaatg aaaaagctgc cctgcattaa
    19381 gtaataagaa catccaagca aaaatagctc ctcatgtttt tgacttggct ggacttatta
    19441 ttgcaaatgt aagcttttga acaccgttta ccaagaggtt gtgaatctgc cgtttaactc
    19501 tgagtcacaa ggaacaattt caaaaaacag cttttcatgt ttgatgaata ttgtgacttg
    19561 tgaccgagaa gttggcagga gaaaaccgtt tatacgaaaa aagggactgt gacaaatcgt
    19621 gtcacagtcc cttttttata gtgtttttta taggtagtat tttttagcaa ctgttaagtc
    19681 atcatttaat tcataaacaa gtggttgacc tgttggaatt tcaagatcca tgatgtcttc
    19741 gtcagagata ccttcgatat gtttagctag ggcgcgtaat gagttaccat gtgcagctac
    19801 taaaacagtt ttgttgtcta gtaatgctgg agcaatttca tcttgccaga atggcaaagc
    19861 gcgttctaat gtcactttca agttttctcc accaggaata tcgcgtttgt ctaacattgc
    19921 gtaacgacgg tcatttgctg cagaaccttc gtcagtttct tccattaatg gaggaagagt
    19981 atcgtaagaa cgacgccaga tgtgcacttg ttcgtcgccg tatttttcag ctgtttcttt
    20041 tttgttcaaa ccttgtaatt taccatagtg acgttcgttt aagcgccatg atttgatttg
    20101 tggtacccaa agttgatctg aatattctaa gatcaagtta catgttttga ttgcacgagt
    20161 taaaacagaa gtgtaagcaa catcaaattc gattccagct tctttgattt tgcgtccgcc
    20221 ttcttttgct tcttcgattc cttctggtgc tagatttaca tcagcccaac cagtaaattg
    20281 gttcaatgca ttccattcac taagaccgtg acgagaaaaa actaattttg gcatgtttag
    20341 ctctcctttt aagataggtt tatatttctg aaattttcag ttctgtttta ttgtacaatt
    20401 ttttggaaga gatttccatc gaaaaattag ataagattct atgagaaaac aagtagagaa
    20461 tataaaaaaa cgaacagtgc ccacgcaact gttcgttttt aggtattaga tgcataattg
    20521 ctcgatttct ttttcaggta aattgatata tttataacat gttccaactg aaacaccgca
    20581 ttttgaagaa ataaattgga tcgtttcttt tttttcataa tagagacgtc ggattttttt
    20641 gatcgttttt gcatcgattt ttggtcttcc gccgattttt ccttttttac gagcttcatc
    20701 taatccaact aatgtacgtt ctttgatcaa tgcgcactcc atgtttgcta ggttttccat
    20761 taattggaaa tagatttttc ccatttcgcg cgtatcgatt ccttcatcaa gactaatcaa
    20821 gtgcagcccg cgatctttga ataattgcgt gagttctgtt aattgccgcg tagatttgcc
    20881 taatcgatga agttggcaaa tgactagtgc atcgccttgt tgcatgtcag cgatcactga
    20941 ctccaaatga acagcagtag aatcattttg ttccacttca tatgtctcat ggaaaatttc
    21001 ttgacaacca aagtcagata attgatcaac ctgagttgtt aaatcaacgt catttatggt
    21061 tgtgcgtgca taaccaataa ttttcatggt gattcctttc tagcagtgat tttctaactg
    21121 gatataacag taaaaatgta gcataaagta cgtcaaaaag atagtttttt cgcaaatttt
    21181 ccgctgtctt tttttattgg atgatcaagc agtttttctt tatttacgac ggattgcgtc
    21241 aagaatctca acgccttttt ctgagccgac aactacttta gaaaccatgt ttaggaaaag
    21301 cccatgttct actaccccaa caagttcatc taaataggta gctaagcttt ctggctgttc
    21361 gatgacttct agatgcagat caatgatgta atgaccacca tctgtggtta agacttctcc
    21421 gttttcgtct gtacgcaaga cgggttggaa tcttttttct tcaaaaatat gaagtagctg
    21481 ttgggagcca taagggatca cttcaactgg gagtgggaac ttcccaagtt tatcgactaa
    21541 tttagaagaa tcaacgatcc aaatcgtttc ttttgagtac gtagcgacta ttttctcgaa
    21601 aagcaaagca gcgccgccgc ctttgatccc ttgaaaatca gcactgattt catcagcacc
    21661 gtctattgtc aaatcgacta cggggacttc atcaatactc tttaacggta ttcctaattt
    21721 ttccgcttgt tcttttgtcg catttgaagt agtaactcct gtgatacgta aaccttcttc
    21781 acgcatacgg cgaccaatct cttctaccat ataataggcg gttgaaccgg tcccgagacc
    21841 tacgatcatc ccgtccttta cgaatttcgc ggcttcaatg ccagctaatt gttttaaatt
    21901 catctttgcg tccacctttt tttagtttga gcatcgtgct ttcctcacta ttttagcgtg
    21961 atttacggta aaaagatacc attctttttt attccaataa agctttttca aaaaaaattg
    22021 catctcttgt tgacatgttt tttcattcgt gatattctaa caaaggtgct ttatctacag
    22081 gtttctgtga agaaacgtgc tgactgttta tcaaagcata tttgataagg atgcagacat
    22141 tgcatttatt ttttatcaca aaaaaagaac cacctggatg tgtggaacta gacgcaaatc
    22201 gtgggaagga ggaaacaagg aatgcctaca attaaccaat tagtacgtaa acctcgtaaa
    22261 tcaaaggtgg aaaaatctaa ttcacctgct ttgaacaaag gatataacag ctttaagaaa
    22321 actcaaacta acgtaaactc acctcaaaaa cgtggggtag caactcgtgt gggtacaatg
    22381 acacctaaaa aaccgaactc agctttacgt aaatatgcac gtgttcgttt gtcaaactta
    22441 attgaagtta ctgcttatat cccagggatt ggtcataact tacaagaaca cagtgttgta
    22501 ttattacgcg gtggtcgtgt aaaagactta ccaggggtac gttaccatat cgttcgcggt
    22561 gctttagata ctgctggtgt taacgaccgt aaacaaagcc gttctaaata cggaactaaa
    22621 aaacctaaag cttaatttta attattaaac aaatgaaact tgcttctact atatagaagt
    22681 aattactatg aacaatattt cggaaggagg agttacggat gccacgtaaa ggtcctgttg
    22741 caaaacgtga tgttttacca gatcctattt ataactcaaa attagtaact cgcttgatca
    22801 accgtgtaat ggttgacgga aaacgcggga ttgcagctaa cattatctat aatgcctttg
    22861 acttgatcaa agaatctaca ggtaacgatc cattagaagt gtttgaacaa gcaatgaaaa
    22921 acgttcaacc tgttcttgaa gttaaagcac gtcgtgttgg gggttcaaac taccaagtac
    22981 cagttgaagt tcgccctgaa cgccgcgtta ctctaggtct tcgttgggta gtaaactacg
    23041 ctcgcttgcg cggtgaacac acaatggaag aacgcttagc aaaagaaatc atggatgctg
    23101 caaacaacac aggtgcttct gttaaaaaac gtgaagacac tcataaaatg gctgatgcca
    23161 accgtgcgtt tgctcactat cgctggtaag attctctctg tttggtcggc ttttataagt
    23221 cgacctcaga tagtgtcctt atttttagca actagatagt aaagagagga gaaacaagga
    23281 gaatgaccag agaattttct ttacaaaaca ctcgtaatat cggtatcatg gcccacatcg
    23341 atgctggtaa aacgactgct actgaacgta tcttgtacta tactggtaaa atccataaaa
    23401 ttggtgaaac tcatgaagga gcttcacaaa tggactggat ggaacaagaa caagaacgtg
    23461 gtattacgat cacttctgct gctactacag cacaatggaa gggtcaccga atcaacatca
    23521 tcgacacacc aggacacgta gacttcacag ttgaagttga acgttcattg cgtgtacttg
    23581 atggtgctgt aactgttctt gatgcacaat caggtgttga acctcaaact gaaacagttt
    23641 ggcgtcaagc aacaacttat ggtgtacctc gtatcgtatt tgcaaacaaa atggacaaga
    23701 tcggtgctga cttcttatat tcagtatcaa ctttacatga ccgtttgcaa gcaaacgctc
    23761 acccaatcca attaccaatt ggtgcggaag acgacttcac aggaattatc gaccttgtaa
    23821 caatgaaggc tgaaatgtat acaaacgatc ttggaacaga gatcgaagaa acagaaattc
    23881 ctgaagaata ccgcgaatta gctgaagaat ggcgcgaaaa attggttgaa gctgtagctg
    23941 aaactgatga agaacttact ttgaaatact tagaaggtga agaaatcact gaagctgagt
    24001 taaaagaagg tatccgccgc gcaacagtta acgtagaatt ctacccagta ctatgtggtt
    24061 cagcatttaa gaacaaaggg gttcaattac tattggacgc tgttcttgat tacttgccat
    24121 caccgttaga tatcccagct atcaaaggga ttgaccctaa aactgatgaa gaagtagaac
    24181 gtcctgcaga cgacagcgct cctttctcag ctttagcatt taaagttatg actgacccat
    24241 tcgtaggtcg tttaactttc ttccgtgtgt actctggtgt tttacaatca ggttcatacg
    24301 tacaaaacgc aacaaaaggc aaacgtgaac gtgttggtcg tatcctacag atgcatgcca
    24361 actctcgttc agaaatctct gaagtttacg ctggagatat cgctgctgct gttggtttga
    24421 aagacacaac tactggggac acactatgtg atgaaaagag tctagttatt cttgaatcca
    24481 tggaattccc agaaccagtt atccaagtcg ctattgaacc taaatcaaaa gctgaccaag
    24541 ataaaatggg tgtggcttta caaaaactat ctgaagaaga tcctactttc cgtgctgaaa
    24601 caaacgtgga aacaggcgaa acaatcatcg ctggtatggg tgaacttcac ttagatatca
    24661 tcgttgaccg tatgcgtcgt gaattcaaag ttgaagctaa cgtaggtgcg cctcaagtat
    24721 cttaccgtga aactttccgt gctggcactc aagccgaagg taagtttgta cgtcagtctg
    24781 gtggtaaagg tcaatacggt cacgtatgga ttgaatttac accaaatgaa gaaggtgctg
    24841 gtttcgaatt tgaaaatgcg atcgtcggtg gggttgttcc tcgtgaatac attccagctg
    24901 ttgaaactgg tttgaaagat gctatggaaa atggtgtctt agctggctat ccattagtag
    24961 atatcaaagc taaactttac gatggttcat accatgatgt cgattcaaat gaaactgcct
    25021 tccgtgttgc tgcttctatg gcattacgtg cagcagctaa gaaagctaat ccagtaatcc
    25081 ttgaaccaat catggctgta gaagttgtta tccctgaaga ttatttaggt gatgttatgg
    25141 gtcacgtaac tgctcgtcgt ggacgtgttg aaggtatgga agctcgcgct ggcggccaac
    25201 aagttgttcg tgcgatggtt ccacttgctg aaatgtttgg ttatgctaca actcttcgtt
    25261 ctgctactca aggtcgtggt acatttacaa tgacatttga ccactacgaa gatgtaccaa
    25321 aatcagtaca agaagaaatc attaagaaaa acggcggaaa agctgaataa tttcttaaaa
    25381 ccggatagtc ctgcaaagag attatccaaa gagactatat ttttaatata gtttcgtgta
    25441 aaataattat cgatgatatg ggcatgtggt gtaccttgtg tacatcatat acctatcaga
    25501 caaataaaaa atatattgta tttttaggag gacattttaa aatggcaaaa gaaaaatttg
    25561 accgttctaa accacacgtt aacattggta ctatcggaca cgttgaccat ggtaaaacta
    25621 cattaacagc tgcaatcaca actgtactat ctaagaaaaa cggcggccaa gctatggctt
    25681 acgatcaaat cgatggtgct ccagaagaac gcgaacgcgg aatcacaatc tcaacagctc
    25741 acgttgagta tgaaacagat acacgccact acgctcacgt tgactgccca ggacacgcgg
    25801 attacgttaa aaacatgatc actggtgctg ctcaaatgga cggagctatc ttggtagttt
    25861 ctgctgctga cggcccaatg cctcaaactc gtgaacacat cctattgtct cgtcaagttg
    25921 gtgttcctta catcgttgta ttcttgaaca aagtagacat ggttgatgac gaagaattac
    25981 tagaattagt tgaaatggaa gttcgtgacc tattaacaga atacgaattc cctggtgacg
    26041 atgttcctgt agttgctgga tcagctttga aagctctaga aggcgacgct tcatacgaag
    26101 aaaaaattct tgaattaatg gctgcagttg acgaatacat cccaactcca gaacgtgaca
    26161 acgacaaacc attcatgatg ccagttgaag acgtgttctc aattactgga cgtggtactg
    26221 ttgctacagg tcgtgttgaa cgtggacaag ttcgcgttgg tgacgaagtt gaagttgttg
    26281 gtattgctga agaaacttca aaaacaacag ttactggtgt tgaaatgttc cgtaaattgt
    26341 tagactacgc tgaagctgga gacaacattg gtgctttact acgtggtgtt gcacgtgaag
    26401 acatccaacg tggacaagtt ttagctaaac caggtacaat cacacctcat acaaaattct
    26461 ctgcagaagt atacgtgttg acaaaagaag aaggtggacg tcatactcca ttcttcacta
    26521 actaccgtcc acaattctac ttccgtacaa ctgacgtaac aggtgttgtt gaattaccag
    26581 aaggaactga aatggttatg cctggcgaca acgtaacaat ggaagttgaa ttaatccacc
    26641 caatcgctat cgaaaacggt actaaattct caatccgtga aggcggacgt acagttggtg
    26701 ccggtgtcgt tacagaaatc aaagcttaat tcacttaagt tttaaagtaa agcaacattt
    26761 attcggacgt aatcgctcgg aacgaaggtc ttaggatctt tgttccgagc ttttttattt
    26821 attgcaggat caggaaaaaa gacaggagta ttataaatag aagaaacacg aaataaaaga
    26881 gtcagtattt tctaaatgaa actttatgaa aacaaaatgt tttttacaag aaaaagcctt
    26941 gctttgtgag tttatatata ttataatgac aaaggtgcgt ttactttagt gaattgcaca
    27001 gacgtaatca gaatgatttc gtcggctaag aaacgagagg ttgcgacacg cccggacgct
    27061 ttgccacgaa cgagcgtgac ggaaattttc gtggagctat gtctactttt caaaataggc
    27121 gaaggaggga acaagatggc aaaacaaaaa attcgtatcc gtttaaaagc gtatgaacac
    27181 cgtattttag atcaatcagc ggataaaatc gtggaaactg caaaaagaac tggagctagc
    27241 gtgtctggtc caattccatt accaactgaa cgcagtcttt acacaatcat ccgtgcgact
    27301 cataaataca aagattcacg cgaacaattc gaaatgcgta cacacaaacg tcttatcgac
    27361 attgtgaacc caacaccaaa aactgttgat gctttgatga agctagactt accatctggt
    27421 gtaaacatcg aaatcaaatt ataaaactaa aacaaaaata atggaggtgt actcatgacc
    27481 aaaggaatct tagggaaaaa agtgggaatg acacaaatct tcactgaatc tggtgaatta
    27541 attccagtaa ctgttgtaga agctactcca aacgtagtac tacaagtgaa aacaatggaa
    27601 acggatggct acgaagctat tcaagttggt taccaagata tgcgtgaagt tttatcaaac
    27661 aaacctgcga aaggtcatgt tgcaaaagca aacacggctc ctaagcgctt cattcgtgaa
    27721 ttcaaaaatg ttgagctagg agaatatgaa gtaggaaaag aaattacagt agatgttttc
    27781 caagcaggag acattattga tgtaacaggt actacgaaag gtaaaggatt ccaaggggtt
    27841 atcaaacgcc acggacaaag ccgcggacca atggctcacg gttctcgtta ccatcgtcgt
    27901 cctgggtcaa tgggtccagt tgcacctaac cgtgtattta aaaacaaacg tcttgctggc
    27961 cgtatgggtg gcaaccgcgt aactatccaa aacttggaag ttgtacgtgt cgatgcagaa
    28021 agaaatgtta tcttgatcaa aggtaatgtt cctggagcta aaaaatcttt aattactatc
    28081 aaatcagctg tgaaagctaa ataattgtag gaagaaagga ggaactaagg aatgccgaat
    28141 gtagcattat tcaaacaaga tggaagccaa aacggtgaaa tcacacttaa cgaagaaatc
    28201 ttcggaattg aacctaatga aagtgttgtt tacgatgcaa tcgtaatgca acgtgcgtca
    28261 ttaagacaag gaacacacgc ggttaaaaac cgtagcgctg ttcgcggcgg cggacgtaaa
    28321 ccatggcgtc aaaaaggaac aggtcgtgca cgtcaaggat ctatccgctc accacaatgg
    28381 cgtggaggtg gcgtagtctt tggaccaaca ccacgttctt acagctacaa acttcctaaa
    28441 aaagttcgtc gtttagcaat gaaatctgta ttgtcagaaa aagttgctga aaacaacttg
    28501 gtagcaatcg aaggcttgaa cttcgacgca ccaaaaacaa aagaattcaa acaagttctt
    28561 gctaacttat ctatcgactc taaagtattg gttgttctag aaaaaggtaa cgactttgct
    28621 gctttatcag cacgtaactt accaaacgtt tctgtagtaa cttctgataa tgtgagtgtg
    28681 ctagacgttg tatcaaatac aaaagtgtta gcaacacaaa ctgctcttac tcaaattgag
    28741 gaggtgcttg cataatgaac ttactagacg taatcaaacg cccagtgatc actgaaaaat
    28801 caatgcttgc catggatgac aagaaataca cttttgaagt ggacactcgc gcaaacaaaa
    28861 ctttagtaaa acaagcggta gaagctgctt ttgacgttaa agtcaaaaac gtgaacatcg
    28921 tgaacgtacg tgcgaaattc aaacgtatgg gcaaatatgc aggatataca aaaaaacgtc
    28981 gcaaagcgat cgttacattg actgaagatt caaaagaaat ccaattattc gaagctgctg
    29041 aataagcact aatagataac tcattaaatt aggagggaaa aacgtggcga ttaaaaagta
    29101 caaacctacc tcaaatggtc gtcgtaacat gacaggttct gattttgctg aaatcacaac
    29161 atcaacacct gaaaaatcat tgttacaacc attgaaaaac catgctggac gtaacaacaa
    29221 cggccgcatc actgtacgtc atcaaggtgg cggacacaaa cgccaatatc gcgtgatcga
    29281 cttcaaacgt aacaaagata acgttgttgc aactgttcaa acgatcgaat atgatccaaa
    29341 ccgttcagct aacatcgcgt tagtacacta tgaagatgga gtgaaagctt acatcttagc
    29401 acctaaagga ctagaagtgg gtatgaaact tgtttcaggt ccagaagcag atatcaaagt
    29461 cggaaatgct cttccattag aaaacattcc agttggtact gttatccaca acattgaaat
    29521 gaaacctggt aaaggtggac aattgatccg ttctgctggt acaagtgctc aagtacttgg
    29581 taaagaaggc aaatacgtat tgatccgctt gaactctggt gaagttcgta tgatcttagc
    29641 tgcatgccgt gctacaatcg gttctgtagg taacgaacaa cacgaattaa tcaacatcgg
    29701 taaagctgga cgctctcgtt ggatgcgtaa acgcccaact gtacgtggta gcgtaatgaa
    29761 ccctaatgat cacccacacg gtggtggtga aggtaaagct cctatcggac gtaaagcacc
    29821 agtttctcct tggggtcaac cagctcttgg ttacaaaact cgtaacaaaa aagctaaatc
    29881 agacaaactt attgttcgtc gtcgcaaaaa taaataatta ctttgaccat gtgtcgcaca
    29941 ttttgagagg aggttcacca tgggtcgtag tttaaagaaa ggaccttttg tcgatgagca
    30001 tttaatgaaa aaagttgaag ctcaagcagg ggttgaaaag aaaaaagtta tcaaaacttg
    30061 gtcacgccgt tcaacagttt tcccaagttt tgttggatat accatcgcag tttatgatgg
    30121 acgtaaacat gtaccagttt acatccaaga agac
//
LOCUS       DACEXQ010000013        29197 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.013, whole genome shotgun sequence.
ACCESSION   DACEXQ010000013 DACEXQ010000000
VERSION     DACEXQ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.013"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..362
                     /locus_tag="IXH04_000426"
     CDS             <1..362
                     /locus_tag="IXH04_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAQ1851060.1"
                     /translation="WTQIKKEYPTYIYCTIPLSEKRRTERGFNQVSEMLKAARIPFQM
                     LLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLFHAAD
                     CLQVAQPKIIRTISLAR"
     gene            490..1041
                     /gene="raiA"
                     /locus_tag="IXH04_000427"
     CDS             490..1041
                     /gene="raiA"
                     /locus_tag="IXH04_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAQ1851061.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            1263..3797
                     /gene="secA"
                     /locus_tag="IXH04_000428"
     CDS             1263..3797
                     /gene="secA"
                     /locus_tag="IXH04_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAQ1851062.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            3923..5024
                     /gene="prfB"
                     /locus_tag="IXH04_000429"
     CDS             join(3923..3985,3987..5024)
                     /gene="prfB"
                     /locus_tag="IXH04_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAQ1851063.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSFAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            5129..5815
                     /gene="ftsE"
                     /locus_tag="IXH04_000430"
     CDS             5129..5815
                     /gene="ftsE"
                     /locus_tag="IXH04_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAQ1851064.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            5799..6692
                     /locus_tag="IXH04_000431"
     CDS             5799..6692
                     /locus_tag="IXH04_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1851065.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            6844..8292
                     /gene="cls"
                     /locus_tag="IXH04_000432"
     CDS             6844..8292
                     /gene="cls"
                     /locus_tag="IXH04_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ1851066.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGGKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            8629..9486
                     /locus_tag="IXH04_000433"
     CDS             8629..9486
                     /locus_tag="IXH04_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAQ1851067.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            9601..10521
                     /gene="pstC"
                     /locus_tag="IXH04_000434"
     CDS             9601..10521
                     /gene="pstC"
                     /locus_tag="IXH04_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAQ1851068.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            10521..11405
                     /gene="pstA"
                     /locus_tag="IXH04_000435"
     CDS             10521..11405
                     /gene="pstA"
                     /locus_tag="IXH04_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAQ1851069.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            11420..12226
                     /gene="pstB"
                     /locus_tag="IXH04_000436"
     CDS             11420..12226
                     /gene="pstB"
                     /locus_tag="IXH04_000436"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ1851070.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            12242..13000
                     /locus_tag="IXH04_000437"
     CDS             12242..13000
                     /locus_tag="IXH04_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ1851071.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            13013..13690
                     /gene="phoU"
                     /locus_tag="IXH04_000438"
     CDS             13013..13690
                     /gene="phoU"
                     /locus_tag="IXH04_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAQ1851072.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            13928..14959
                     /locus_tag="IXH04_000439"
     CDS             13928..14959
                     /locus_tag="IXH04_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAQ1851073.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSFLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            14956..15723
                     /locus_tag="IXH04_000440"
     CDS             14956..15723
                     /locus_tag="IXH04_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAQ1851074.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            15823..17391
                     /gene="liaX"
                     /locus_tag="IXH04_000441"
     CDS             15823..17391
                     /gene="liaX"
                     /locus_tag="IXH04_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAQ1851075.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQHQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            17691..18008
                     /locus_tag="IXH04_000442"
     CDS             17691..18008
                     /locus_tag="IXH04_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAQ1851076.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            18008..18358
                     /locus_tag="IXH04_000443"
     CDS             18008..18358
                     /locus_tag="IXH04_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAQ1851077.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            18525..19463
                     /locus_tag="IXH04_000444"
     CDS             18525..19463
                     /locus_tag="IXH04_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAQ1851078.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            19478..20308
                     /locus_tag="IXH04_000445"
     CDS             19478..20308
                     /locus_tag="IXH04_000445"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAQ1851079.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            20352..21377
                     /locus_tag="IXH04_000446"
     CDS             20352..21377
                     /locus_tag="IXH04_000446"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAQ1851080.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHHASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            21392..22330
                     /gene="galU"
                     /locus_tag="IXH04_000447"
     CDS             21392..22330
                     /gene="galU"
                     /locus_tag="IXH04_000447"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAQ1851081.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKINHNPKGITEIKEEKKK"
     gene            22455..22895
                     /locus_tag="IXH04_000448"
     CDS             22455..22895
                     /locus_tag="IXH04_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAQ1851082.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTQDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            22898..23500
                     /locus_tag="IXH04_000449"
     CDS             22898..23500
                     /locus_tag="IXH04_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ1851083.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSSELTGIAKEKAQGLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(23563..24666)
                     /locus_tag="IXH04_000450"
     CDS             complement(23563..24666)
                     /locus_tag="IXH04_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ1851084.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGTDSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVIWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            24876..25877
                     /gene="ccpA"
                     /locus_tag="IXH04_000451"
     CDS             24876..25877
                     /gene="ccpA"
                     /locus_tag="IXH04_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAQ1851085.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            26232..26627
                     /locus_tag="IXH04_000452"
     CDS             26232..26627
                     /locus_tag="IXH04_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851086.1"
                     /translation="MPKKRRKTIVIILTILTIGIGGKFYMDKREMQRQKDLLAVEKQS
                     VKVLKNTFADIKEVKIEKSARNEMTCSYRIVILMTNKQDQSIYFSYSFWKERNEIGSY
                     GIVDEKKQKEGNTLNKVKVTYSNGNEESI"
     gene            26627..26878
                     /locus_tag="IXH04_000453"
     CDS             26627..26878
                     /locus_tag="IXH04_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851087.1"
                     /translation="MITDKQYSELSDAVYWLDPKHRDYVPEMQENLSFKINGTMYKIL
                     KIKNSFDGMQAMAVAPIVHSKLEKNFKNKKIPANFRVLK"
     gene            26921..27316
                     /locus_tag="IXH04_000454"
     CDS             26921..27316
                     /locus_tag="IXH04_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851088.1"
                     /translation="MSRKIRKLSLAILLIVILGIGGKVYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIREVKVEEFKKNPVTGSYGALVTMTNNEGKSVYFDYSFWKERNELGGF
                     GIENRAVQKTGVTIKRVKVIFSNGQEELV"
     gene            27313..28569
                     /locus_tag="IXH04_000455"
     CDS             27313..28569
                     /locus_tag="IXH04_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HAQ1851089.1"
                     /translation="MTINDKHYNDISEKVYWLDPKYPRYNEGYKKNSVKEFAGMEFQI
                     LQIKDSLDGMQAMSVAPIKNGKVDLSQVVIAFAGTNLSDWKDLETDARSIMNLYGNRP
                     GASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALY
                     VAAEKQWKNVGFNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEV
                     RISMETAFLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQE
                     LNNIRKKLRLSGGQLTVNEQIYLDDTQARLVVEKLSAKMKSAMEEVIRINQRGIFELE
                     KKWQEGLRQARVVAPSLTEQEIIEALASVGATKENLVDSAKEVYLLRINKARRMGEAF
                     DTLAKEIQDKINEIVARDRELAQQFN"
     gene            28581..28904
                     /locus_tag="IXH04_000456"
     CDS             28581..28904
                     /locus_tag="IXH04_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851090.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            28897..29163
                     /locus_tag="IXH04_000457"
     CDS             28897..29163
                     /locus_tag="IXH04_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851091.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQIWAEKRLTDQNYQ
                     MTAEEAAQEFSNRHVHLKGSVKGQFAEKLTELFQRQEKKLKQFR"
BASE COUNT         9852 a         5016 c         6296 g         8033 t
ORIGIN      
        1 aatggacgca gataaaaaaa gaatacccga catacatcta ttgtacaata cctttatctg
       61 aaaaaaggcg aacagaaaga ggattcaatc aagtaagcga gatgttgaaa gcagcaagaa
      121 ttccttttca gatgctactc aaaagagaga agcatctgct ggctcaagca aaaaacacgc
      181 gaaaggaaag attggagatg cctcaacctt ttgaacttgc agtcaccttt tctaagatac
      241 aaaataaaaa aatattgatt attgatgatg tctatacaac aggacagact cttttccacg
      301 cagcagattg tttgcaggtc gcacagccga aaatcatccg aaccatttct ttagcaagat
      361 gaattagatg tggaaatttg atagcgattt aatttgcaaa taacctagag ttcgatataa
      421 tagaattaag aagagggaaa gagtggtcct tctccttctt gattgtggta gacgaaaggg
      481 gtaattttta tgtttagata taatgtacgc ggcgaaaata tcgaagtaac tgaagcgatt
      541 cgcgactacg ttgagaaaaa agtaggcaaa ctagaacgct atttcagtga cgcacccgat
      601 gcaacggcgc atgtgaactt aaaagtttat acagaaaaaa cagcaaaagt ggaagtaacg
      661 atcccattac cttatcttgt attacgtgcc gaagaaactt ctccagattt atacgcaagt
      721 atcgatttgg ttgttgacaa attggaacgc caaatccgca aattcaaaac aaaaattaat
      781 cgtaaatccc gtgaaacggg catgaatact gcagatgcta ctgctttatt caatgaagag
      841 gacgaaaacg aaaatgattc tgaattagat atcgtacgta cgaaacgcct ttcattgaaa
      901 ccaatggata gtgaagaagc agtcttacaa atgaacatgc tgggacataa cttctttatt
      961 tttgaagatg cagaaacaaa tggtacaagt atcgtttacc gccgtaaaga tggaaaatac
     1021 ggactgattg aaacagactg atcagcctta cttaaataac aacgaaaact tgcagcagct
     1081 actgacagct gccgcaagtt tttttgagtt tcctcctctt ttagatgaaa tatgaataaa
     1141 ttatgaagtt cttactttaa tacgcttaag atatttcaat tatctataac taatgataaa
     1201 atgaaaagta aagtctacat agtctggata atacacagaa ggaaaaggag attacacaac
     1261 gcatggccaa tttgattcgt tcacttatcg aaaacgataa gaaagaacta aaacgtttag
     1321 atagcattgc aaaaaaagtt gaagcatacg ctgaccaaat ggcagcatta actgatgaac
     1381 aattacaggc aaaaactgct gaattcaagg gcagatacca aaaaggagaa acattagatc
     1441 aattacttcc ggaagctttt gcagtcgtcc gagaagcagc aaaacgtgta ttggggttat
     1501 atccacacca cgtacaattg atgggtggaa tcgtgcttca cgatggaaat atccctgaaa
     1561 tgcgtactgg tgaaggtaaa accttgaccg caacgatgcc agtatatcta aatgctctga
     1621 ctggcgaagg ggtacacgta gttaccgtca atgaatattt agcaacccgt gactcgacgg
     1681 aaatgggcga actttataat ttcttaggat taacagtggg gttgaatatc aactcaaaat
     1741 cttcggatga aaaacgcgag gcttataatt gtgatatcac ttatagtacg aataatgaat
     1801 taggatttga ctacttgaga gacaatatgg tcgtttaccg ccatcaaatg gtacaacgtc
     1861 cgctgaatta tgccattgtc gatgaagttg actcaatctt gattgatgaa gcgagaacgc
     1921 cgctgatcat ttcagggcaa gcagaaaaat ccacagcttt atatacaaga gcagacaact
     1981 tcgtgaaacg tttgaaagaa gaagaagatt ataaaatcga tgtacaatca aaaacaatca
     2041 gtttaaccga agcagggatc gaaaaagcag aagaaacatt cggtttggat aacttatacg
     2101 acattgaaaa tactgcattg acccatcact tggatcaagc attgagagcc aactatatca
     2161 tgcttttaga tatcgattat gttgtccaag acggaaaagt actgattgtt gaccagttca
     2221 ctggtcggat catggatgga cgccgttatt cagatggact tcaccaagca atcgaagcta
     2281 aagaaggcgt agagatcgaa gacgaaacga aaaccatggc gacgatcact ttccagaact
     2341 atttccgtat gtacaagaaa cttgcgggaa tgactggtac agctaaaaca gaagaagaag
     2401 aattcagaga aatttataac atccaagtat accagattcc gacaaatcgt ccggttatcc
     2461 gtgatgatcg tccagatttg ctttacccaa cattacaaag caagttccac gctgtcgtgc
     2521 aagatatcaa agatcgctac cgcaaaggtc aacctgtgtt agtcggaact gttgcggttg
     2581 aaacatctga gttattatca gatatgctga atcaagaacg tattcctcat gaagtgctga
     2641 atgcgaaaaa tcactttaaa gaagcagaga tcatcatgaa tgctggtcaa aaaggtgctg
     2701 tgacgattgc taccaacatg gctggtcggg gaacagatat caagttagga cttggggtaa
     2761 gagaacttgg tggtctagca gttatcggga cagaacgaca tgaatctcgt cgtatcgata
     2821 accagcttcg aggacgtgca ggacgtcaag gagaccctgg tatgtcacag ttctacttgt
     2881 cattggaaga tgatttgatg aaacgattcg gttcagaacg aatcaaatct ttcttagatc
     2941 ggatgaagat cggtgaggaa gatgctgtta tccaaagcaa gatgctgacg aaacaagtag
     3001 aatctgcaca aaaacgtgta gaaggaaata actatgacac acgtaaaaac gtcttacaat
     3061 acgatgatgt gatgcgtgaa caacgtgaag taatctataa acagcgacaa gaagtaatca
     3121 tggaagaaaa aagcttatcc aagccactaa tgaacatggt caaacggaca atcagtcgtg
     3181 tagtagatgc gcatacacaa ttagagaaag aaaattggaa tttggaaagt atcatcgatt
     3241 ttgccggcag tactcttgtc catgaagata gtattactct tgcagatatc gaaggaaaaa
     3301 cagctcagga gatcaaagag tatctaaacg aacgagcaca agaagtattt aataccaaag
     3361 cggctcaact aaatggtcca gagcaactgt tggaatttga aaaagtcgtg atcttgcgtg
     3421 ttgtggactc taaatggaca gaccatattg atgcaatgga tcaattacgt cagtcaatcg
     3481 gcttacgtgc ttatggacag aacaatccat tagtcgaata tcaaacagaa ggctataaga
     3541 tgtttgaaga tatggtaggc gccatcgagt atgaagtcac tcgaatcttt atgaaagctg
     3601 agatccgtca aaacgtacag cgtgaacagg tttctcaagg aacagcttct cactcagaag
     3661 acggagatgc caaagaaaat agtaatacta gcgcgaaaaa acagccaatc catgtgaaca
     3721 aggtcggaag aaatgatcct tgtccttgtg gaagcgggaa aaaatataag aactgtcatg
     3781 gaaaaaatgc aaactaattt ctgacagcag gattggtgag acaaattctt tttttaagaa
     3841 ttcgtctcac cttctttctg ttatttgctg attctgttag actagatata atgaaaaaag
     3901 tatagtgaaa gaaggaaaaa acatggaaat atccgaaatt cgaaatctcc tgacagaaat
     3961 gcaggaaaag gtcacaagct tcaggaggtc tctttgactt agaccagctg gaagaagaca
     4021 ttgcggaagc agaagcaaaa atggctgaac ctggtttttg ggatgatagc gaagctgctc
     4081 aaaaagtgat cagtgaaaac aatgcaaata aagaaactta tgatcaattc cattcttttg
     4141 ctgaagaact ggaagaaatc gaattgatgg tggagatgtt gcaggaagaa cctgatgcag
     4201 ccatgcaaga agaagcagaa acacgtatca atgcgctaga tgaaaaaatg aagacatatg
     4261 aattatctat gttgttagat ggtccttatg atcgaaacaa cgccatcgtt gaattgcatc
     4321 caggggctgg cggaacggag tcccaagatt ggggcagtat gcttttacgt atgtatacac
     4381 gctgggcaga gcagcatggg tatcaagtag agacactaga ttatcaagca ggtgatgaag
     4441 caggaatcaa gagtgtgacc ctactgatca aaggacacaa cgcatttggt tatctgaaat
     4501 cagaaaaagg tgttcatcgc cttgtacgta tctcgccttt tgactcagcg aaacgacgtc
     4561 atacttcttt ttgttcggta gatgtgatgc cagagcttga tgaaacgatc gatatagaaa
     4621 tcaatccaga tgatttgaaa atcgatacgt accgagcaag tggtgcaggt ggtcagcaca
     4681 tcaataaaac agaatctgct gtacggatca ctcacttgcc tacaggaaca gtagtagcta
     4741 gtcaggcaca acgttcacaa ttgaaaaacc gtgagcaagc gatggggatg ttaaaagcta
     4801 agctttatca attagaagtg gaaaagaaag aacaagaagc agctgcactt cgaggagaac
     4861 aaaaagaaat cggctggggt tcacagatcc gttcttatgt tttccatcca tattctatgg
     4921 taaaagacca tcggacaaac gtagagacag gaaatgtcca agcagttatg gacggtgacc
     4981 ttgatttatt tattgacggc tatttaaaaa tgcatctaca ataagaaccc attgttaaat
     5041 tgaaataaaa ttgtaagatt ttgcaagcat gttgaaacat tgccatgcta taatagctaa
     5101 agactgaaaa agatatggag agaacgccat gattgaaatg aaggatgtaa tgaagaagta
     5161 ctcaaatggt acgaccgcta tccgcaatct ctcaattgtc atcgaacaag gagagtttgt
     5221 ctatgtggtt ggtccgtctg gagccggtaa atctacattc atcaaattga tgtatcgaga
     5281 agaaaaagct tcaaaaggaa ctttgaacgt cgcaggtcat gatctaatag caatcaaaaa
     5341 ccgcgaagtt ccttatctac gcagagaaat cggcgtggtg ttccaagact ataaattatt
     5401 accaagaaaa acagtatatg aaaatgttgc gtacgctatg caagttatcg gcagaaaacc
     5461 tcgggatatc aaaaaacgag taatggaagt gctggacctt gttggtttga aacacaaagt
     5521 tcgagtcttt cctagcgaat tatccggtgg ggaacagcaa cgtgtctcta ttgctcgggc
     5581 aatagtgaat acgcctaaag tacttatcgc ggatgaaccg acaggaaatc tggatccaga
     5641 aaactcatgg gaaatcatga aattattaga tcggatcaat gcccaaggaa ctactgtcgt
     5701 gatggctaca cataacagta cgatcgtaaa tacgattcgg caccgagtaa ttgctatcga
     5761 aaacggccgc attattcgag atcaagcgga aggggaatat ggatacgatg attagaacct
     5821 ttttccgcca tttattagaa agtatcaaga gtttgaaacg taacggctgg atgacagttg
     5881 cttcagtcag tgcggtaacg atcacattga ctttagtcgg tgtattcatg gcagttatca
     5941 tgaacgcaac taaattagct caagatatag aaggaaacgt tgatgtctct gtattcgtag
     6001 atatcggtac aaaagacgaa gagatgaaaa agctagaaaa agaattgaat gacttagatc
     6061 atgttaaaaa agttgaattt tcaagcaaag aacaggaatt gaaaaaaatc caaaacgaaa
     6121 tgggtgattc ctggaaattg tttgaaggtg acaataaccc gttatatgat gtgtatgtag
     6181 taagtgctac tgatccttct tatacaaaac aagtagcaga agaagcagca gatttgaaga
     6241 atgtttctcg ttctgattac ggtggagctt cctcagatcg gattttccaa atcgctggtg
     6301 cagttcggac atggggatta gttgcagcgg cattgctgct gttcgtagcg atgtttctca
     6361 tttcgaacac gattcggatc acgattttgt ctagacagcg tgaaattcaa atcatgcgtt
     6421 tagtcggtgc aaaaaatgga tatattcgct ggccgttttt ccttgaaggt ggctggatcg
     6481 gtctgatcgg tgcgattctt ccgattttga tcatcacatt tggttatgca tggtttttcc
     6541 aattgatcaa tccatcatta ttacgttccc attattcatt gattcatcca caaaatatcg
     6601 tttggaaaat caatctttta atggtcggca ttggtgtgat cattggttca cttggctcga
     6661 tcatttctat gagacgtttc ttgaaattct aataccacaa tataaataaa atgagaaagt
     6721 tggacagttt cgttccagct ttcttttttt attggctagg agaatagaag gaagcggctt
     6781 ttttcccttt actttaaaaa acaacttggg tattcttaag ttggtagaag aagaggtgaa
     6841 gccgtgaaaa ttatcgttga caattttttt acgatccttt tgattatgaa tattttgctt
     6901 tcctttatca tcgtttttcg agaaagaaag gaaactgctc aaacatgggc atgggtgctc
     6961 gttttgatgt tcattccagt tgtaggattt attttatata tttttttagg aagagggatt
     7021 tccaaagaga aaatctttga tttgaagatc caagggaaaa tcggtaagaa cctggaaatt
     7081 gaagaagaca aacaggcatt gatgcgaggg ctttatcctc atccgcctac tggacaagta
     7141 gatgtgaaac aattgatcta tatgctcacg gtatttgaaa gtacgctcta tacgactaaa
     7201 aatgaaatca ctttatttac agatggtcgt gaaaaattcg atgcgctgat tgaagatatc
     7261 aagcaggcag aagaccatat ccacttccag tattatattt atcgcagtga tgcattaggt
     7321 gaagaagttc gagatgcatt gacagaggcg gcaagacgag ggggaaaagt cagagtcctt
     7381 cttgatgcat ggggttccac acaagtttct tccagctttt ttcagaattt gaaaaaagca
     7441 ggcggagaag tcgcattttt ctttcctcta tttgttccgt atattaatcc tcggatcaat
     7501 tatcgcaatc atcgtaaaat cgtagttatt gatgggaaaa tcggatatac aggtgggttc
     7561 aatgttggaa atgaatatct aggattggta aagaaatttg gttattggcg agacaatcat
     7621 ttgcgtattt acggagaagc ggtgtatacc ttgcaaaacc gttttttgat ggactggaat
     7681 tctcagcata caaaagaagt aggctattct cccaaatttt ttccgtccat tcactctact
     7741 ggtgatatag ctgttcaaat cgtgacaagc ggaccagata ccgaacatga acaaatcaaa
     7801 atgacttatt tgaaaatgat ttcgttagcc aaacgggaga tattgatcca aacgccttat
     7861 tacataccgg atggatcgat tcatgaagca ctgaagttgg ctcttctttc aggcgtaaaa
     7921 gttcatatcc aaatacctaa caaaccagat catcttctcg tttactgggc aacgtattct
     7981 ttcgctgcag agctaatcga gtatggagca aggatcgaaa catatgagaa tggatttatt
     8041 catgcaaaga cgatgattat cgatggtggc attgtttcag ttggctcagc aaatatcgat
     8101 gttcgttcat ttcgcttgga ctttgaggtc aatacactgg tttatgatga acgaatggct
     8161 tcacgtgtaa gaaaagcttt ttttgaagac tcgaaaattt ctacgcatct cacaaaagaa
     8221 atgtatgaaa accgaggaat aatcatcaag atgaaagaag gattagcaag attgatttcg
     8281 ccgctccttt aaaacataaa aaggacatga gaaccatcaa cagatggaga tctcgtgtcc
     8341 ttttctaacg gggccatttc tgagagtacg tcagaaatgg agaaagcgtc tcgcatgagc
     8401 gaaattaact ttggggtatc tcttgttttt tggggaaagc aagaaataag ctaatatatg
     8461 ctattaaaag agacacattt caaagccagt atagcataga aacttttaat gagatagttt
     8521 tttttacaat tatgagaatt tattttgtta tttaatgatt ttgattcgtt ttcatcaagt
     8581 aatgaaaatg ataaaataaa catactttag gattaaagga gatttctaat gaaaaaactt
     8641 aaattattct taccaataat aggattagct attcttttat caggctgttc aaagtggatt
     8701 gatcgaggag aatccattac ggctgttggc tcttctgctt tacagccttt agtagaaaca
     8761 gttgctgaaa cttaccagac agaaaatcct ggaaaattca tcaatgtaca agggggcgga
     8821 agcggaactg gtttgtctca agtacagtca ggagcagtcg acatcggaaa ctctgatttg
     8881 tttgcggaag aaaaatcggg gatcgatgcc tcagcattag tcgatcatcg agtagcagtt
     8941 gttgggatca ctcctattgt gaacaaggat attggcgtaa aagacatcag tatggaaaac
     9001 ctgaaaaaac tatttacagg cgagatcaaa aactggagtg aagtcggtgg aaaagatcag
     9061 ccagtagtta tattgaaccg cgcttcagga agcggaaccc gtagcacgtt tgaaaaatgg
     9121 gtattggacg gagaaacagc tgttcaggca caagaacaag attcaagcgg aatggtgcgc
     9181 cagatcgttg ccgacacacc cggtgcaatc agctatgttg ctttttctta tgtttccgat
     9241 gatgtagata cgttgaatgt taacggtgta gaaccgacag atgaaaatgt gacaacgaac
     9301 aaatggacga tttgggcata tgagcatatg tacacaagag atcatccaaa aaaactgaca
     9361 aaagaatttt tagactatat cttatctgat gaagtacaag ataacatcgt tggagaacta
     9421 ggttatatcc ctgtttctca tatgaaagtc gaacgtgatt ggcaaggaaa tatcgttaaa
     9481 gagtaatctt tacacaaaat ttacaaactt agctaaactt ttacatgtta ttaaaaccaa
     9541 attgatgttc aatttgtaca ctgagtgagt atgttaaacc tgattaaggg ggagtcatct
     9601 gtggaagatg tgaaaaaagt attattaaca aaatcaaaac gttcaaaaat ggaacagcgt
     9661 ggaaaattca tcagtttttt atgtatcgcg ctgatcgtgt tggttgtatt atctattttt
     9721 tattttgtag ctagtaaagg attggccacc ttttttactg ataagatcaa tgtatttgat
     9781 tttctgtttg gtacagattg gaatccaagc caaatcggag caaatgggaa accaatggta
     9841 ggggcattgc ctatgatcac aggttcgttt attgtgacct ttttatctgc tatcgttgcc
     9901 acgccatttg ccattggtgc agcagtattc atgacggaga tctctcctaa aaacggaacg
     9961 aagatcttac agccggtcat cgagctgctt gtaggtattc cttctgtcgt ttatggtttt
    10021 atcggtttat cagttatcgt gccttttgtc agaagtattt ttggcgggac tgggtttggt
    10081 atcttagctg gtacatttgt gttgttcgtt atgatcttgc cgactgtcac aacgatgact
    10141 gttgatgcat taaaggcagt gccacggcac tatcgggaag cttcattagc gcttggtgcc
    10201 acaagatggc aaacaatcta caaagtcgtg ctacgagcag ccgtgcccgg gattttaaca
    10261 gcagtcgttt ttggtatggc tcgtgcgttt ggtgaagcct tagctatcca aatggtcatc
    10321 ggtaatgcag cattgatgcc aacaggtctt actactcctg cttctacatt gacgtccatc
    10381 ttaacaatgg gaatcggaaa tacaatcatg ggaacagttg aaaataatgt gttatggtca
    10441 ttagcgctga tcttgctatt gatgtcatta ttctttaata tcgtgatccg tatgattggg
    10501 aagaaaggag agctgaaata atgaacgcaa aacgctcaga taaaattgct acaggtgttt
    10561 tatatatagt ttcggggatc atcgtattga ttttagcagc tttactactt tatatcttag
    10621 ttcggggaat accgcacatt tcatggtcct tcttgaccga accttccaaa gcctaccaag
    10681 aaggcggtgg aatcgggatc cagctattta actcgattta tttgcttttg atcacgatga
    10741 tcatcagttt tcctatttct ttaggtgcgg gtatctatct ttcagaatat gctggaaaaa
    10801 actggctaac agatgtgatt cgcacgtcga ttgaaatctt gagctcgttg ccttctgtcg
    10861 ttgtcggttt attcggtttc ttggttttcg ttattcaatt ccagtatggt ttctcgatta
    10921 tttctggtgc attggctttg actgtattca acttgcctct tttgactaga acagttgagg
    10981 agtctttaca agcggtccat tacacacaac gagaagcagg attggcctta ggtttgtcac
    11041 gctgggaaac agtgataaaa gtggtggttc cagaagcgac accagggatt ttgactggtg
    11101 tgatcctaag ttctggaaga atttttggtg aagcagctgc attgatctat acggctggac
    11161 aaagtgcacc tgcgttagat tttacgaact ggaatccatt aagtgtttca agcccgatca
    11221 gcattttccg tcaagctgaa acattagctg tccatatttg gaagatcaat acagaaggca
    11281 cgatgcctga tggtgtttct gtttcggctg gcgcttctgc cgtgttgatt attgctgttt
    11341 tactgttcaa cttcggcgct cgtgcgattg gcaaacgatt ataccgaaaa atgacttcag
    11401 cctaaggaga agaagcaaaa tgaaaaaata taactggaat gaaacaaatg tcatcaccat
    11461 gccaaaagag aaagaagtag cactatatac ggatgatcta catgtctggt atggcgacaa
    11521 tgaagcaatc aaaggagtgg atctggaatt tgaaaaaaat aagatcactg cactgattgg
    11581 tccttccggc tgcggaaaat ctacgtatct tcgttcgttg aatcgaatga atgatgggat
    11641 cgctaatacg aaaatcactg ggaaaatcat gtatcaaggc gtagatatta atgcgcctca
    11701 agtggatgtt tatgagatgc gcaaacggat tggcatggtt tttcaacgtc ctaatccgtt
    11761 cagcaagtcg atttatgaca atattacatt tgctttgaaa cagcacggcg aaaaaaacaa
    11821 aaagaaatta gatgaaatcg ttgaaacaag cttgaaacaa gctgccttgt gggaccaagt
    11881 aaaagacagc ttggacaaaa gtgccttggc tttatctggc gggcaacagc aacgtttatg
    11941 tattgctcgt gcgatcgcga tgaaacctga tatcttgctg ctagacgagc cggcaagtgc
    12001 tttggaccct atttcgacag gaacggttga agaaacactt gtaaacttga aagaagacta
    12061 tacgatcatc atcgtgacgc ataacatgca acaagcagct cgtatcagcg actatactgc
    12121 ctttttctat ttaggaaaag taatggaata tgacaaaact aacaagatat ttaccagacc
    12181 aaaaattcag gcaaccgaag attatgtgtc cggtcatttt ggttaagacg gaggcctcaa
    12241 catgacaaaa gagattattt catctaaaga tcttcatctg tattatggaa agaaagaagc
    12301 actaaaaggg atcgatctaa gcttcaatca aggggaaatc actgcgatga tcggtccttc
    12361 tggttgcgga aaatccactt atctgcgttc tttgaaccgg atgaatgacc tgattccgaa
    12421 tgtaacgatc accggaagtg ttttatataa agacaaagac atctatggtc caaaaacaga
    12481 tgttgttgaa ctaagaaaag aaatcggcat ggtattccag cagccaaacc catttccttt
    12541 ttctatctat gaaaatgtca tctatggatt gaaattaaaa ggggaaaaag ataaaaaagt
    12601 ccttgatcaa gtagttgaag aaagcctgaa ggcagcgtct gtttgggatg atgtgaaaga
    12661 caaattgcat aaaagtgctt tatctttatc cggtgggcag cagcagcgtg tatgtatcgc
    12721 acgtgttttg gcagttaatc cggaaatcat cttattggat gaaccaacaa gtgcgcttga
    12781 tcctgtttct actgggaaaa tcgaaagtat gctgttagaa ttaagagagc ggtatacgat
    12841 gatcatcgtg acccataata tgtctcaagc ttcacgtatt tcagataaga cggcattctt
    12901 tttagatggt cacttgatcg aatacaatga tacgaagaag atattcatga atccagaaaa
    12961 acaagagaca gaagattata tttcaggtcg atttggataa ggaggaaacg gaatgctacg
    13021 tacacaattt gaagaagaat tgctgaatct ccataatcag ttctacgaaa tggggatgat
    13081 ggtgagcagc gccgtgcaca agtcagttcg tgcatacatc aaacacgata aaatcttagc
    13141 ccaagaagtt atcgaaaacg atataaatat caataacatg gaaacacgct tggagaagaa
    13201 aagttttgaa atgatcgcat tgcaacagcc agttacaact gatttgcgta tgatcattac
    13261 ggtcatgaaa gctagctcgg atttagaacg tatggccgat catgcggtgt ctgttgctaa
    13321 atccacgatt cgcgtaaaag ggcagacgag aataccggaa atcgaaaaag agatctccga
    13381 tatgtccgat tatgtgaaaa aaatggtgga caacgtccta gttgcttatg tcaaaacaga
    13441 cgaaaaagac gcacgtacga tcgccaatat ggatcaaaga gtcaatgact atttcaatcg
    13501 aatctataat gcaacaatca aaaatatgca ggaaaatcct gagacagtca tcagtggaac
    13561 agactacttg aatgttgcca gttatctaga acggatcgga gactatgtga caaacatctg
    13621 tgaatggatc gtctacttgg ctacagggaa aatcactgag ttaaatacga accacaatga
    13681 gattttctaa actgaagctg tgattatgcc gtttgtttag gaaaagaggt tgtgaaatca
    13741 gcggttgtga aaaagctgtt ctgcatcaag taataagaac agtcaaacaa aaaatagctc
    13801 ctcatatttt tcgtttggct ggacttatta ccgcagatgc aagcttttga acaccgttta
    13861 ttcgggaaag ggtttgtgac aaaacatgtc acaaaccctt ttcatcattt catagaaagg
    13921 ataaagaatg caaaaaaaaa ttagcatcat cactatattt tttatttgtg tgcttacttc
    13981 atgggttatt cagcatcaag ccgctttata tgaagtacct gtcatcgaga tagaaaaagt
    14041 acgaataaaa gaggatcatc aattgattac aggaaaactg atgaacacgg atcaaaaagg
    14101 agaatcgatt tatttatcgg ttccttatca aaaaaatcaa atagaaacag caaatataaa
    14161 agcgagacaa atgtatctag tccaaatgca tggagatgaa tggcagctag tagcaaaaaa
    14221 aaatgatggc tggcttttct tcttcacaag tatcttcatt ggcgccatgt tgcttattgg
    14281 tggaaaacag ggtctatttg ctttaggaag tctcatcatc aatatcgttt tacttttact
    14341 tttgttgctc atctttacta aaacagaagg gatttctcta ttaaatatat ctatcctgtt
    14401 cgtcgttata gggatgatta ttagcatatt ggctttagat ggatggaata gaagcagtat
    14461 aatcaaaatt tcagctgcag ttacgagtgt ttttttagca ttttttattt gtcttttgac
    14521 aatgaatcat tggaaagacc aaggactgcg ctatgaggaa ctgaattatc tgacacgacc
    14581 gtatcgttct gtttttcttt ctagcgtact gattggaaca gctggaggaa cactcgatac
    14641 cgttatcaca gtcattgcga cattagaaga attgactaag cgacaaccag aaataacagc
    14701 gaaacagtta tggctttcag gaaggcaagt gggtcgagat gtgattggct cgatgaccaa
    14761 tatccttttg ttcgtctata tcagtgggtc agttccatcg tttcttttat atttaggaaa
    14821 tcaatggtcg ttcaaagaaa cgatagaaca gcatttatca ttggaatttt tacgcgtcat
    14881 tgctggcagc ttaggaatag tattatcgat ccctatctcg gtcctctttt ttctgactgt
    14941 aaggaggctg aaacgatgaa tgtacttatt atacttgttg ggattttagc aatcagcgtg
    15001 ctacttattg gcggtacgca aggtatgtat attcttttag gcttattcat caatttaggg
    15061 atctttttcc tgttgttatt cggttatcat cagaagtggc cgatactggc attgtcggtc
    15121 ataggatttt tattgattgc tgtggttatc ttatttttta tcaacggtta taatctaaaa
    15181 atgcgtgcag cttttgtgag tatactgatc tttttatttt gtttcctact attgcttcct
    15241 atcactgatt ttttagcaat ccaaggtttt acatcggtag aactagaaga attatctggc
    15301 ttagataaga ctttagcgat tgattttaga ttacttacga gatcgttgct tctgattagt
    15361 cttagtggtg ctgttttaga tgcgagtgta gcaatttcta gtgggacatt cgaagtatat
    15421 caggcaaatc cgcaattatc tttttaccaa ttgcgccatt ctagtttcgc tattgctaaa
    15481 aaaatattgg cgtcaacagt aatgacactt ctttttgcat tcatgggaag ttctttagcg
    15541 ttgatcctct ggtttattga tttagacatt ccttttcagc aagtgatcaa tgaaaaatca
    15601 tttgttctag aatatacgat ggctattctt accacattgt ccgcactact tgttcttcct
    15661 ttaacctgcg tgactacagc atatctcttt acaaaaaaga aagaacaact caagatggag
    15721 taaaagttac aacttaagtc ctatgacatt tttactgaat cagtgcataa tatggatgta
    15781 aggaaaagct tgcaaacaat tttgcttaag gaggcaattt ccatgaaaga aagagaacgt
    15841 attttagatt tagtgaaaaa aggaatcctt tcgactgaag aaggcttaga tttattagaa
    15901 agcatggcaa ctgaaaaaga cgaaaagcaa atcaaaaaag aagcagacaa ggtaactgct
    15961 agtcataaag aaaaagatca ggcaagtcaa ttgatcgata aacttgaaaa cggcgaagaa
    16021 gaaatttctg agccagttga tccaaaggaa agagaaagac aagatcaaga aaacttagaa
    16081 aagatcttag atgaactagc aactgaagcg aataaaactt cggcgcgtct agatgaagta
    16141 aatgctcaat tcgctgacaa taaggcagca cgcaatgaga aacaggaagc attgatgcag
    16201 ctgaatacaa aagaagaatt aggcgagttg acagaagaag aattagcaca gcatcagacg
    16261 ttagaagcag aaatcaaaga attagaagct agtggcgata cactgttaga agaacaattg
    16321 aaattagaag cagaattaaa agatatccgt aagaatcaat ggtctgagaa aaaagaaacg
    16381 tttactgata agtttgaatt gccagatgat tggaaagatc aagcaacaga tacactgaat
    16441 caagtaggcg aaaaaatgtc agaagctgga tcacagttag gcaaattctt gaagaaaaca
    16501 ttccaaactg tttcagaaac agtgaatgac aacatggaat ggaaagatgt cagcttacgt
    16561 gtccctggga ttgcgacaac gaaatttgag catgaatttt actatgaagc tcctgcagca
    16621 agtatccttg atatcaaggc agcaaacgga aacgtgacgt taaaaacgtg ggacagcaac
    16681 gatgtgaaag ttgaagctaa gatcaaactt tacggaaaaa tgggtgctga accatttgaa
    16741 gcattctctg aacggagcca gattgaggtt aatgaagatc atatctcctt ccaaattcca
    16801 aataaacgtg tgcgtgcaga ccttgttttc tacttaccta aacgagtata cgaccacgca
    16861 gctattaaac ttctaaatgg caacatcatg attgaaacat tggaagctaa agatatttat
    16921 acaaaatcta cgaatggtaa catcatcgtg gaccaattaa ctgcaacgat gctggaagta
    16981 gaaggtgtaa acggcaatat cgatattcga aacgggaaca tcttagattc tattatcgaa
    17041 acagtaaatg gtacagtgac atttggtgcg acagcagaaa acctttctgt gtctttggta
    17101 aatggtgatg tacgtcttac gattaaagaa gataatttga aaaaagtaga agcaagttca
    17161 gtgaacggaa atgtcaaagt cgctttacca gatggcatcg gtttggaagg acatgctaaa
    17221 acaagtcttg gaagtatcaa cagccgtctg tccaattacg aagtagttcg tgagaaaaaa
    17281 gaacggacaa accaaatgtt gcaattccgt cgtgtttcag atggtgaaat cgcgcaagtg
    17341 caactttcaa cgacaacagg aagtatctat ttaaaagata cagataaata aagaggttgt
    17401 gaaagagatg ttcagccttg agcaataaga agcgattttg taaaatagct tcccctattt
    17461 tacaaaattg tgccttattg ccgatagtct atctcttgaa caccgtttac caagagatta
    17521 tgaacctgcc aattatcgcc gagtcacaag gaataatttc agaaaaagct cgtcacgttt
    17581 tatgaatatg gtaatttgtg accgagaatt ttgcagaaag acaccgatta agaagaaggt
    17641 tgtcacaacg attttcttca tttaaaatag tagaacagag gtgagacatg atgaaaaaaa
    17701 agttgacaaa atctaataaa aatgttgttt tgacagggac tttagcagga ataggagaat
    17761 acatcgggat tgatccgact gttatccgtg tgctttatgt attcctgagc ttagttgctt
    17821 ttggaagtcc aattatttta tacattttat tagctttgat tatcccatct gcgcgttctg
    17881 cacgaacaaa ctatggacat aacaatgcat attatgaaaa aaacaattat cgtgaacaaa
    17941 ctcgtgaaca aacaaaaaaa cgcaaagaag cggaaaaaat cgatgatgac gaatggagtg
    18001 acttttaatg tcgtactttc aacgtatttt agttaatacg ttaacattca tatcattggc
    18061 tgttcttttt cctgcgatgg tccatgtgag cagtatttgg acggccgttc ttgccagctt
    18121 cgtattgtct gtgttgaata tgtttgtcaa accgattttg acgatactgt ccctgccctt
    18181 tacattagtc acattgggat tgtttacgtt tgttatcaat gcagcgattc ttaaaatgac
    18241 ctcctttttt gtgggagcag aacattttag tttttcttca ttcggtgcag cgttgttagt
    18301 agctatcttg atgtcgcttg tcaatgtaat tgtcagcgaa cataacagta gaaaataaaa
    18361 gaaagctgtg ccgaactatc ggcgcagttt ttttatttag aatcaggaat gcgttttcta
    18421 ttacattggt aattaaataa aaacgttttt tttaattggt agtacttttg gctagacaat
    18481 gataaaatga tagagaacac tctaatagaa atgaggtagc atgaatgtca gaagttgtaa
    18541 aagttagcca attagtgaaa aaattatctt tggaaatcgt gacaggcgac gagaaaagct
    18601 tagaccgtgt catcgtaact ggggacatct cacgcccagg actagagttg actggttatt
    18661 ttaattatta ttcccataat cgtttacagc tatttggcag taaagaaatc agttttgcgg
    18721 aacggatgat cccagaagag cgtttgatca tcatgagaag attatgtagt gaagatatgc
    18781 ccgcatttat tatttcaaga ggattaccag ctccagaaga aatgatccag gcagcaaatg
    18841 aaaatgggat agctgtactg cgttctccca tctcaacttc tcgtttatta ggagaattat
    18901 ccagttattt agatggtcgc cttgcccctc gaacaagtgt ccacggtgtt ttagtggatg
    18961 tttacggatt aggtgtactg attcaaggtg acagtgggat aggaaaaagt gaaacagcgt
    19021 tggagttgat caaacgcggc catcgattga tcgcagacga tcgagtagat gtttatcagc
    19081 aagatgaatt aacggtgatc ggagaaccgc ctaaaatcct ggaacatctg attgaaatcc
    19141 gaggaatcgg gatcattgat gtaatgaatc tttttggtgc gagtgcggta cgtggctcta
    19201 tgcaagtcca gttggctgtt tatctggaag catgggcaaa agataagaaa tacgaccgtc
    19261 tgggctctga agacacgacc gttgaaatcg cggaagtggg cattccacaa ataaaaatcc
    19321 ctgtcaaaac aggacgaaac gttgcaatca tcattgaagt agccgcgatg aatttccgtg
    19381 cacgtacaat gggctttgac gcaacgaaaa catttgaaga aagattaagt cgcttgattg
    19441 aagaaaactc aggcaacgac tagcaaggag tgacaatatg ttagcacaaa tcaatcgagt
    19501 tgcttttgag cttgtaggga ttcctatcta ctggtatgca ttgatcatcg tatcaggtat
    19561 cattatcgct atgtggctta gcagcagaga ggctgttcgg gtaggattaa aagcagatga
    19621 tgtgacagat tttatgttgt ggggattgcc tatctccatt attggcgctc gcttatatta
    19681 tgttttattt gatcttcccc agtatatagc tgatccaatc caaatattca atatccgttc
    19741 aggaggctta gctatttatg gcggactgat tgcaggcgcg atcacgctgt atttttttac
    19801 taaatatcat tttatttctt tgtggacttt tttagatatc gctgcaccaa gtgtattatt
    19861 agcacaggca atcggtcgtt ggggaaattt catgaaccac gaagcatatg gaccagacac
    19921 gacgcgaagc ttcttagaaa gtctgcattt accgcaattc atcattgata atatgtatat
    19981 cgaagggatc tatcgtcaac caacatttct ttatgaatca ctttggagtt tgattggatt
    20041 tattttgctt ttgtttttac gaaagaaacc acaccttttg aaaaaaggcg aagtcctttt
    20101 atgttatcta atgtggtata gtttcgggcg tttctttatt gaaggattac gtacagacag
    20161 tctttacttt ttaggtgtga tccgtatttc tcaattatta tctgctgtgt tatttgtcgg
    20221 tacattgatt ctatttattt ggcgcagaaa aaaaggaaat ttaccggatt atgaccgctc
    20281 attaggaaaa aatcaacaaa ttatctgatt tttagagcgt taaaaatgaa aaaaatttca
    20341 ggagtggtaa aatgaattat agagtagcgg ttttaggacc aggctcttgg ggtactgctt
    20401 tggcacaagt cctcgccgaa aacggacatg acgttcggat ttggggacat catgcttcac
    20461 aagtagaaga aatcaacacc caacacacga atcaacgtta tttaccagat cattatctac
    20521 ccgattccat ccaagcattc accaatatgg aagatgcagt aaaagacgca gatgcggtgc
    20581 tattcgtcat tccaacgaaa gcaatacgtt ctgttgcaaa agaactagtg cctaaattga
    20641 atacaaagcc agtgatcatc catgcaagta aaggattaga acagggaaca cataaaagga
    20701 tctctgacat cttgttagag gaaatcccag cagaaaaaag aaaagctgtt gttgttcttt
    20761 ctggaccaag ccatgcagaa gaagtagcaa aacatgacat cactacgatc acagctgcaa
    20821 gtacagatgc aaaagcagca aaatatgtac aagagctgtt catgaataat tattttcgga
    20881 tctataccaa tcctgatgtg atcggtgtag agatgggggc agctttgaaa aatattattg
    20941 ctattggtgc tggcgctatt cacggcttag ggtttggcga taatgcaaag gcagcaatca
    21001 tgacgcgcgg attagctgag atcagccgtt taggtgtagc aatgggtgcc aacccgctga
    21061 cctttatcgg attaagcgga ataggcgatc tagtcgttac ttgcacaagt gttcattcta
    21121 ggaactggcg cgcaggcaac ttgttaggac aagggcagcc cctagaagag gtgctagcca
    21181 atatgggaat ggtcgttgaa ggtgtgaata ctacccaagc agcgatggaa ctagcccaaa
    21241 tgatggaaat cgaaatgccc atcactcaaa caatctatga agtgctgtat gaacaaaaag
    21301 aaatcaaaac agccgcaaaa gaaatcatgt tacgtgatgg aaaaaaagaa aatgaattct
    21361 cattaacaat tttttgaagg agcgaatgta tatgaaagtt aaaaaagcag ttattccagc
    21421 agcaggatta ggaacaagat ttttaccagc aacaaaagct atggcaaagg aaatgttgcc
    21481 aattgttgat aaaccaacaa tccagttcat cgttgaagaa gcgttgaaat caggtatcga
    21541 agacatcttg atcgttacag gaaaagctaa acgaccaatc gaagatcact ttgattcaaa
    21601 tattgaatta gaaacaaacc taagtgaaaa aggaaagacc gaattattga aattagtaga
    21661 agaaacgaca gatgtcaact tgcatttcat ccgtcaatct catccaaagg gtttaggaca
    21721 tgccgttctt caagcaagag cctttattgg aaatgaacca tttgtcgtta tgctaggcga
    21781 tgatataatg gaagacgaag taccattgac acagcaattg atcgatgact atgagcgtac
    21841 acacgcttct acgattgccg ttatgcaagt tcctcacgat gaaacttcaa aatacggaat
    21901 catcaaccca ggggaagtac tggaagatgg gttatacaat gtcaaaaact ttgttgaaaa
    21961 accagatcca agcaaagcac caagtgattt agctattatt gggcgttact tattgactcc
    22021 agaaatcttt gatgtattat cagaacaaca accaggtgct ggaaatgaga tccaactaac
    22081 tgacgcaatc gatacgttga ataaaacaca acgcgtattt gcccgtaaat tcacaggtaa
    22141 acgttatgac gttggtgaca aatttggttt tatgaaaaca agtattgaat atggtctgac
    22201 acatccagaa gtaggggcac ctttgcgtga atacatcatg tctttaggta aaacattaga
    22261 agcagaagga agcaagatca atcataatcc aaaaggaata acagaaatca aagaagaaaa
    22321 gaaaaaataa tctttttttc taacgagctt cagcggctaa ttgaataaaa agtagttgaa
    22381 gctcgttttt atttgtttaa aaaacacgaa aatgctatcc ttatagaaaa gagagacaag
    22441 aagggagttt gatcatgtca ggaggagaaa ttgcaggatt gatcgcagca atcgcctttg
    22501 cgatacttgt cgttttcatc gttcgcgtat tgttgcaagt ttccaaaact ttagaaaaag
    22561 tagaccagac tgtcgcagaa gcaaatacga caattgagat cgtcacacaa gacgtagatc
    22621 ttttatctag acaagtagaa ggcttgctag tcaaaagtaa tgagctgctc ttggatatca
    22681 atggaaaagt cgcaacgatc gatccattgt tcacagcagt tgctgattta agtgaaagtg
    22741 tatcggaact taatcattct agtaaaaaca tcgtgacgaa agtcggcgga atcggaaaaa
    22801 cgacagctaa agcgactgtt gccggaaaag tcggtggaac agcgatgaaa ttttttaaaa
    22861 acaaaaaaca tactgaaacg acaggaggaa aataagaatg gctaaaaaag gtggattttt
    22921 attaggtgca gtagttggag gtacagccgc agcaatcgcc gcattattgt tagcacccaa
    22981 atctggtaaa gaacttcggg atgaattggc tgctcaagca gatgatttga tggacatggc
    23041 ttcagattac acagatatgg caaaagaaaa atcatctgag ttaacaggta ttgcaaaaga
    23101 aaaagcgcaa ggtctttctc aacaagcagg ggatctagca ggcaatgtta agcagaaggt
    23161 aagtgaatca gcagaggaag caggaagtga atcagatgat atcttgttaa acctcaaaga
    23221 acaatcagct gatttatcta atcgtttcaa aaaaacagca gaagatctga aaaagccagc
    23281 agacgaatta ggtagtattg ctaaagatac ttcagaagat atcatgatcg atgtaaaaga
    23341 tgctgcagat aaagcaggag agacggtcaa tgaaggaatg tcagaagcaa aaccagttgc
    23401 agatgacaca aaagaaatgt tgaaaatggc aaaagaagat gcaaaagaag ctaaaaaaga
    23461 aatgaaagat acaatgacag attcgactac caatcactaa gattactcat ttctagaata
    23521 aaaaaggtga gagcggacca gctctcacct tttttatttt cattactcta aaacaagtaa
    23581 ttcttttggt gtagtcgtga atggcacaca accatcactt gtcacataca cacaatcttc
    23641 gatacgtacc cctacttttt caggaatata gattcctggt tcaagagaga agcacatccc
    23701 ttcttcaatc actaagtcgt ttccttctac aagagaaggg aattcgtgga cggttgttcc
    23761 gattccgtga cctaaacggt gattgaaata ttcgccgtag ccatagctgt tgatcacgtt
    23821 tcgtgcgatt tgatcaagtt cacctgctgt cacacctggg cgaactgcct cttgtgcggc
    23881 aagctgtgct tcaagagtga tgttatagat tttttcttgg aactcagttg gtttttgata
    23941 agcaactgta cgagtagcat cactgcaata gccattccaa ataacgccaa gatcgaataa
    24001 cactagttca tttggtgaaa ccttcgtgct tcctggtgta ccatgagggc ttgcagcatt
    24061 tgctcctgca aggactaatg tgtcgaaaga catttgtgaa acaccttgtt tttttaattg
    24121 gtattcgatt tctgcgataa ttgcttgttc agttacgcct tcttttactg ttttgaagcc
    24181 aatttcaaag gcaacatcgg cccatttacc agcttcgatc agtttgtctt tttcttctac
    24241 cgttttgatc agttgcagac gctggataat agaagttaga tcggtgtaag aatctgtacc
    24301 tgtcaagata tcagagatag catcgaagcg atcgacagtc agagcattct tttcaatacc
    24361 tacattacca ggttgcccat agcgtgcgcg aagctctttt ccgatgatct cccaaggatt
    24421 ttcgctgtct aagtagccac gtacatcgta cgcccagctg ctgttttgag catcttcaac
    24481 ttccagagca ggagtgaaaa ggaaaggatc ttgttccaga gggataaaaa gagctagtac
    24541 acgctcgtga ggatcacttt catatccgga aaagtaagcg atgtggccag ggtcgctgat
    24601 ataagccaat tctacttggt gttgtgccat ccattgacgt agttcattga tttttacgtt
    24661 attcatttgg caacctactt tctaaattaa ttttgtattc aataattgta ccactgattg
    24721 attaaaagac caacgttctt aaaactgaaa aaagtaaaaa ttttcatgaa aacagagata
    24781 aaacggttgc aattgttgag attatttgat atcctatttt agaaatgtaa gacgtatttt
    24841 cagaaaacat ataaaatatg aataggagaa cacaaatgga aaagcaaaca atcactatct
    24901 atgatgtagc gagagaggca aatgtttcga tggctactgt ttcacgtgtc gtaaatggca
    24961 atccaaacgt caaaccagca acaagaaaaa aagtcttgga agttatcgat cgcttggatt
    25021 atcgtccaaa tgcagtcgca cgtggcttgg caagtaagaa aacaacaaca gttggggtaa
    25081 tcattcctga tgtcagcaat atgttctttg cctctttagc acgtggtatc gacgatgtgg
    25141 caacaatgta caaatacaat atcatcttag caaactcaga tggcaacgat caaaaagaag
    25201 tcaatgtatt gaataattta ttggctaagc aagtggacgg cgtgatcttt atgggtcacc
    25261 atatcacaga cgaaatccgt ggagaatttt cacgttcaaa aacgcctgtt gtactagctg
    25321 gttccattga tccagatgaa caagtaggta gcgtaaacat tgattatgtg tcagcaacaa
    25381 aagatgcagt aaataaatta gcaaaaagcg gcaataaaaa aatcgctttt gtcagcggtg
    25441 cgctgattga tcctatcaat ggtcaaaacc gtctaaaagg ctataaagaa gcattaagcg
    25501 aaaacaatct accatatagc gaaggattga tttttgaggc acaatacaac tttaaagatg
    25561 gtctgtctct agttgaccgg atccataata gcggtgcaac agctgtctat gtttcagacg
    25621 atgaattagc cattggtatc ttggatggtt tattagaccg cggcgtaaaa gtaccagaag
    25681 aattcgaaat catcacaagc aacaattctt tactgacaga agtagctcgt cctcgtttga
    25741 caagtatcac acaaccatta tacgatttag gagcggtatc catgcgccta ttgacgaaaa
    25801 tgatgaataa agaagaagtc gaagaaaaaa cgattatctt gccttatgga atcgaagaaa
    25861 aaggatcaac taaataataa aagatcaatc atttaaaggt tgttccaaaa gtggtcaact
    25921 tctggaacaa cgtttatacg agaaaggggg ttatgacaaa attttgtcat aacccctttt
    25981 tttttgtgac tttaaaaaaa gagaaaatgg cggaaacaaa ccttacgtcc atcgtatacg
    26041 acacaatctg tgcgtagatg ctacattttg ttcactacta tcaaaattac tttaaaatga
    26101 aaacgtgata aaaaagaaag tagaaaagta gataagtaac tctaaaaaat ccaaaaaaat
    26161 ccaggctcga ttgcgtactt atcttaaagg agaaacaatc catcatatat aacccgaaag
    26221 gagacttcta tttgcctaag aaaagacgaa agaccatagt catcattttg accattctta
    26281 ctataggaat cggagggaaa ttttacatgg acaaaagaga aatgcaaaga cagaaagatt
    26341 tactcgcagt agaaaaacag tccgtcaaag ttctgaaaaa tacctttgcg gatatcaagg
    26401 aagtgaagat tgaaaaatct gctcgaaatg aaatgacgtg ttcataccgg atagttattc
    26461 taatgactaa taaacaagat caatcgattt atttttctta tagtttttgg aaagaaagaa
    26521 atgagatagg aagttacgga atagttgatg aaaaaaagca aaaagaagga aatacgctca
    26581 ataaagttaa agtaacttac tctaatggaa atgaggaaag tatttaatga ttactgacaa
    26641 acaatatagt gaattaagtg atgctgttta ctggctagat cctaaacata gagactatgt
    26701 acctgaaatg caagaaaatt taagttttaa aattaacggc actatgtata aaatcctaaa
    26761 aatcaaaaac tccttcgacg gcatgcaggc gatggctgta gcgccaatcg tccatagcaa
    26821 attggagaaa aatttcaaaa ataaaaaaat tcctgcaaac tttcgcgtac ttaaataatg
    26881 aaggtgtcaa tacttacata aaaagaaagg aggcacgttc ttgtcaagga aaataagaaa
    26941 actcagctta gccattttat taatcgtcat attagggatc ggagggaaag tctatatgga
    27001 caaaagagaa gcacaaaggc agaaagattt actcgcagta gaaaaacagt cagtcaaagt
    27061 tctgaaaaat acctttgcgg atatcaggga agtgaaggta gaagaattta aaaaaaaccc
    27121 tgttaccgga agttatggtg cacttgtgac aatgacaaat aatgaaggga aatctgttta
    27181 ttttgattat agtttttgga aggagagaaa tgaacttggt ggttttggca ttgagaacag
    27241 agcggtacaa aagacaggag tcactataaa aagagtaaaa gtaatttttt ctaacggaca
    27301 ggaggagtta gtatgacaat aaacgataag cattacaatg atattagtga gaaggtttat
    27361 tggctagatc ccaaatatcc ccgctacaat gagggataca aaaagaactc tgttaaagaa
    27421 tttgctggta tggaatttca aatcctacaa atcaaagact cattggacgg catgcaggcg
    27481 atgtctgtag cgccaatcaa gaacggtaaa gtggatcttt ctcaagtagt gattgctttt
    27541 gcaggaacga acttaagtga ttggaaagat ttggaaacgg atgcaagaag tatcatgaat
    27601 ctctacggga atagaccagg agcaagtttt tctgaaaaag caacaacaaa caagatttta
    27661 tccgttagtg gattgaaaac agcggggcaa atcaattcag cgattgcatt tgctaatgaa
    27721 gtaaaaaagg aatatggaga agctgctatt tctactaccg gccattcatt aggtggattc
    27781 ttagcattat atgtagcagc agagaaacaa tggaaaaatg tcggcttcaa cgggccggac
    27841 ccgtatgatc tcttatcacc agaagcaaaa aaatgggtgg aagaaaatcc cggtatgctc
    27901 accaattacc gaaatcacgc cgattttatc ggtaattttg gtggtaatgg cacaggtgct
    27961 gaagttcgga tcagtatgga aacagcattc ttgaatctgc ttgcttccca cagtttaacc
    28021 tcttggaagt ttgatgaaaa tggaaagctg attattcctg aaaatgaaaa caatgttaaa
    28081 gctcgaaaac aacagaaaga aaaccaagcg acccagttgt tttcctatca gttgcaagaa
    28141 ctgaacaaca tacgtaaaaa actacgatta agtggcggcc agctgacagt taatgaacaa
    28201 atttacttag atgacacaca agctaggtta gtagtggaaa agttatcggc aaaaatgaaa
    28261 agcgctatgg aagaagttat ccgcatcaat caaagaggaa tttttgagct agagaaaaaa
    28321 tggcaagaag gcttgcggca agcacgtgtt gtagcccctt cgttgactga acaggaaatc
    28381 atagaagcat tagctagtgt gggcgccaca aaagaaaact tagtcgattc tgcgaaagaa
    28441 gtctatctgc tacgaatcaa taaggcaaga cgtatggggg aggcctttga tacattggcg
    28501 aaggagatcc aagacaaaat caatgaaatc gtggcacgag atcgagaatt agcacagcaa
    28561 ttcaattaaa ggagaagtag atgaaactag aagaattgga aagagattat cgaagtgaac
    28621 gaagacaatt agaagctcaa gaagatgaat tgatccactt taagaatcaa ggattacgat
    28681 tgatggagca agaggagcag cgactgcatc atgtattaaa gaacttagct ttagataatg
    28741 agcctctgtt tcaagcaaga cgaagttatt cgaattatca ggagcattat caacaagccc
    28801 tacaagtaga acgacgaaaa ctctatcagc agcaagatga actagaacgt tcctatcgtc
    28861 aaaaaatcag acaaataact atggagaaat catcttatga atgaattttt aatgcaatta
    28921 ctaaaagaat tagagaaaaa tctacatcga tcaaatgtac gttcagttgg tatggggata
    28981 tctcagcaaa tttgggcaga aaaacgattg acagatcaaa actaccaaat gactgcagaa
    29041 gaagctgctc aagaattttc taaccgacat gtacacttga aaggatcagt aaaagggcaa
    29101 tttgcagaaa aactaactga attattccaa agacaagaaa agaaattaaa acagtttcgt
    29161 tagtaaagtt atatagaaaa attttgtgaa aatggtt
//
LOCUS       DACEXQ010000014        29171 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.014, whole genome shotgun sequence.
ACCESSION   DACEXQ010000014 DACEXQ010000000
VERSION     DACEXQ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29171
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.014"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..1031)
                     /locus_tag="IXH04_000458"
     CDS             complement(<1..1031)
                     /locus_tag="IXH04_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1851092.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFATSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1113..1802)
                     /locus_tag="IXH04_000459"
     CDS             complement(1113..1802)
                     /locus_tag="IXH04_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ1851093.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="IXH04_000460"
     CDS             complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="IXH04_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAQ1851094.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2329..2832)
                     /locus_tag="IXH04_000461"
     CDS             complement(2329..2832)
                     /locus_tag="IXH04_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAQ1851095.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="IXH04_000462"
     CDS             complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="IXH04_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAQ1851096.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="IXH04_000463"
     CDS             complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="IXH04_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAQ1851097.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4800..5468
                     /gene="sdaAB"
                     /locus_tag="IXH04_000464"
     CDS             4800..5468
                     /gene="sdaAB"
                     /locus_tag="IXH04_000464"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAQ1851098.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5481..6353
                     /gene="sdaAA"
                     /locus_tag="IXH04_000465"
     CDS             5481..6353
                     /gene="sdaAA"
                     /locus_tag="IXH04_000465"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAQ1851099.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6444..7463)
                     /locus_tag="IXH04_000466"
     CDS             complement(6444..7463)
                     /locus_tag="IXH04_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ1851100.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7636..8523)
                     /locus_tag="IXH04_000467"
     CDS             complement(7636..8523)
                     /locus_tag="IXH04_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ1851101.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="IXH04_000468"
     CDS             complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="IXH04_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAQ1851102.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9246..9416)
                     /gene="secE"
                     /locus_tag="IXH04_000469"
     CDS             complement(9246..9416)
                     /gene="secE"
                     /locus_tag="IXH04_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAQ1851103.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="IXH04_000470"
     CDS             complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="IXH04_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ1851104.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9754..10398
                     /locus_tag="IXH04_000471"
     CDS             9754..10398
                     /locus_tag="IXH04_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ1851105.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10454..10957)
                     /locus_tag="IXH04_000472"
     CDS             complement(10454..10957)
                     /locus_tag="IXH04_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ1851106.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10970..11016)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11554..12519
                     /locus_tag="IXH04_000473"
     CDS             11554..12519
                     /locus_tag="IXH04_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAQ1851107.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12618..13790)
                     /locus_tag="IXH04_000474"
     CDS             complement(12618..13790)
                     /locus_tag="IXH04_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1851108.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13777..14922)
                     /locus_tag="IXH04_000475"
     CDS             complement(13777..14922)
                     /locus_tag="IXH04_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1851109.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14936..15868)
                     /locus_tag="IXH04_000476"
     CDS             complement(14936..15868)
                     /locus_tag="IXH04_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1851110.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15985..17064
                     /locus_tag="IXH04_000477"
     CDS             15985..17064
                     /locus_tag="IXH04_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ1851111.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17061..17690
                     /locus_tag="IXH04_000478"
     CDS             17061..17690
                     /locus_tag="IXH04_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1851112.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(17800..18027)
                     /locus_tag="IXH04_000479"
     CDS             complement(17800..18027)
                     /locus_tag="IXH04_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ1851113.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18130..20718)
                     /locus_tag="IXH04_000480"
     CDS             complement(18130..20718)
                     /locus_tag="IXH04_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAQ1851114.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20738..21397)
                     /locus_tag="IXH04_000481"
     CDS             complement(20738..21397)
                     /locus_tag="IXH04_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ1851115.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21446..21949)
                     /gene="trmL"
                     /locus_tag="IXH04_000482"
     CDS             complement(21446..21949)
                     /gene="trmL"
                     /locus_tag="IXH04_000482"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAQ1851116.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22170..23036)
                     /locus_tag="IXH04_000483"
     CDS             complement(22170..23036)
                     /locus_tag="IXH04_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ1851117.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23145..23786
                     /locus_tag="IXH04_000484"
     CDS             23145..23786
                     /locus_tag="IXH04_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAQ1851118.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23848..25209)
                     /gene="mgtE"
                     /locus_tag="IXH04_000485"
     CDS             complement(23848..25209)
                     /gene="mgtE"
                     /locus_tag="IXH04_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAQ1851119.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25227..26126)
                     /locus_tag="IXH04_000486"
     CDS             complement(25227..26126)
                     /locus_tag="IXH04_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ1851120.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26128..26925)
                     /locus_tag="IXH04_000487"
     CDS             complement(26128..26925)
                     /locus_tag="IXH04_000487"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAQ1851121.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26903..27607)
                     /locus_tag="IXH04_000488"
     CDS             complement(26903..27607)
                     /locus_tag="IXH04_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAQ1851122.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27738..28313
                     /locus_tag="IXH04_000489"
     CDS             27738..28313
                     /locus_tag="IXH04_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAQ1851123.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28431..29108
                     /locus_tag="IXH04_000490"
     CDS             28431..29108
                     /locus_tag="IXH04_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAQ1851124.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8590 a         6175 c         5245 g         9161 t
ORIGIN      
        1 aatacagtat tatttcggtt gcttgctgct tcacctagcc cttgtactcg tgtttgattg
       61 gcgacttgac caaattgatt cccgacgtct aataaaataa tagctaaaag caaaatggcc
      121 aaatggcttc cgccaaccaa taaagcaata aagcttattg tttgcataac aagtcccata
      181 tacacgattt ttctttcaga ataagaatct gccagtcgtc ccacgatagg tgcagcaact
      241 gctccagaca aaccaaaaat cgctaatatc cctgcttcga atgttcccca attgtaggcg
      301 ggactgctga tataaaaaat caacgtagac caaaagatcg aaaatgttcc aaacatgaaa
      361 aatccactga ctgccgcttc tcttaataga cgttgagaag caactaaatg cggcaaactt
      421 ttaagtgaat caaggtatga aacagctgta ctactttgct ggacagaacg cggcattttc
      481 cagtaaagca gaagtgtaag aattccgact gcacataaag caaataaata gatcatccgc
      541 caagaaaaaa gactggcaac aataccagat accgtccgag aaagcaaaat ccccacgagt
      601 aaaccgctaa gcaatgtacc catgaccttc cctcttgctt ttggtcccgc tagtaccgca
      661 ccgtaaggaa tgatgatttg cggcacgatc gaaagtaatc ctattaaaaa agaagcgcat
      721 gcaaataacg taaacgaagt cgcaaaaaaa gcggccaaca gagagacggc agataacgct
      781 accattcgaa tgatcaattt tggacgatcc accacgtccc ctaaaggaac taaaaacagt
      841 aaccctaacg catagcctaa ttgggtcaac atcgtcaata tcccgactgc acttgtgcta
      901 acagataaac tagctgctac ttttgttcca atcggctgga tgtaatacat attggcaaca
      961 acaacgccac aagtgattga taatagtaaa ataagcgaag ttgttaaagg tgttttttca
     1021 ttactcttca ttgatttccc ccatttccta tcttagttta caacttatgg aaaagattgg
     1081 aaatagaaaa atacaactat tcgccacact tattatttgt gcttttgttt tccttgtttt
     1141 ttcttgcgat ctttataaat gaagacaact aaccctacta gcacagtaac acttgcaacc
     1201 aacgtgactg cagttttgtt atatccgtga aacagagctt ctccaccaca cgcatacaac
     1261 acagacgaag ggatgactcc aatcacgatc gacaatatga tttgctgtag aggcaatttc
     1321 atcgcatctg cagcaaaatt gaccatagaa gatggaataa ccggtatcat atagcctaac
     1381 attacaccta tttttggatg tttcatttgt cggattgctt gcacccaatg attcgtttcg
     1441 gttttcttat ctaaaaattt taagcgatgc ataaggaaaa ttgcagtgat gttaccaaga
     1501 gcattaccca aaatgttgat gatcgcacct gtcagtggtc caaagctcaa accagcaatg
     1561 actccaacta ctgaagtagg aatacctgga acagcacaca tcaccgctgt caatacgatc
     1621 aagagtatcg atgcaaaagt cccatgcgaa cgaatatgtg cgagtagctc tgccttactc
     1681 gctctcggat ttaaaaataa ttggatgtct gtctgatatt ctaaatacaa ccgatagccc
     1741 actaccagta tcagaatgat tcccaacgag atcaacatca atcggattat tttctttttc
     1801 atatttcctc ctgaaaaaaa tctatattca ttaaataata gtttaacata aaaacaaaaa
     1861 agcaccgaaa tccaacatag gatctcagtg cttttaaata gtaaaaatta tttaacagtt
     1921 acagatgcgc caacttcttc aagtttagct ttcaattctt cagcttcttc tttagatacg
     1981 ccttctttaa ctggtgcagg agcgccatca actacagctt tagcttcttt caagcctaaa
     2041 ccagttgctt cacgaactgc tttgataact ttaacttttt ggtctccagc tgcagttaat
     2101 tctacagtga attctgtttg ttcttcagca gcagcagcgc caccagcagc agctacagct
     2161 acaggagcag cagcagatac gccaaattct tcttcaatag ctttaactag gtcgttaagt
     2221 tctaggatag ttgcttcttt taactcagca acgatgtttt caatgttcaa tgccattata
     2281 atttcctccg tttaattgtt cttttttatt taatgcttta tgcggcaatt aagctgcatc
     2341 tccatcttct ttgtctgcca ctgctttgac agcgtaagcc acgttgcgga ctggcgcttg
     2401 tagtacagat agcaacatag atagaagtcc ttcgcgattt ggcaattttg ccaatgctgt
     2461 gatttcttcc aatgaagaga ctttaccttc gatgataccg cctttgattt ctaatgcttt
     2521 tgcttcttta gcaaattcgt ccatgatttt cgctggtgcg actacatctt cgttactgaa
     2581 tgcaacagct gtaggtccag tgaatacttc gtccatgcct tcaaggccag cttttttagc
     2641 tgcacgtgaa agaatagaat ttttgataac tttcatttct acgttagcat cacgaagttg
     2701 tttacgtaaa cgtgttactt catcaactgt taatccgcgg tagtcaacga ctactactga
     2761 tgcagctgct gtaaattttt cgcttacttc atcaacgatt gctgcttttt ttgcgataat
     2821 tgcttcactc atttttgagt ttcacctcct gaattttgat ggaagagttt tttgcactga
     2881 aaaggcaagt cacttttata gttactttgc tcaaccagta ttattcatca cctattcact
     2941 tcgttcatag tgattctact cgctttgctc gaaaggaaac tcctttcgct ttgctcaacc
     3001 agtattgttc atcatctctt cacttcgttc atagtgattc acaataaaaa actctatgcc
     3061 accgcagaca tagagggact attgaatata ttcaataaac gtcctcggta ggaaattaag
     3121 gcttgatgcc acctactgtc ttcggtacaa gttaattatt aacctcaact agcttagcat
     3181 gctaaactag ttgagtcaag aattattttt cagttggatc aatcaacatt ctaatattta
     3241 attagaaaga agcttggtca acatggatac caggtccaaa tgttgttgtg atcgttacat
     3301 ttttcatgta ttgaccttta gctgctgatg gtttagcttt tagcaataca tcatggattg
     3361 ctgcaaagtt ttcaactaat ttttcatcgt caaatgaaac ttttccgatt ggtacatgga
     3421 tatttccagc tttgtccaca cggtaagtta ctttaccagc tttcacttcg tttactgctt
     3481 ttgttacgtc cattgtaact gtaccagttt tagggtttgg cattaagcct tttggtccta
     3541 atactcgtcc tagacgacct acagtagcca tcatatcagg tgttgcaaca actacatcaa
     3601 agtcaaacca tccgttttgg attttttgaa ccatgtcgtc gtcacctacg aaatctgcac
     3661 cagctgcttc agcttctttt gctttgtctc ctttagcaaa aactaaaaca gtttgtgttt
     3721 taccagttcc gtttggtaat acaacagcac cacggatttg ttggtctgct tttttagggt
     3781 ctacgttcaa acggtatgca acttcaactg ttgcatcaaa ttttgcaatg tttgtttctt
     3841 ttgctaaagc tactgcttcg gcaacaggat aaactttact tgaatcaact ttttttaatg
     3901 catcttgcat ttttttgctc tttttagcca ttttggttcc tccttgattg tggttataac
     3961 ggttgtacct cccacgtgtt ctatatcttt caatatagag ccaactgaga agctgctgtc
     4021 ttagggtgta tagattattc tacagtgatc cccatgcttc gtgcagttcc ttcaaccatg
     4081 cgcattgctg cttcaacgtt agctgcgttt aggtcttcca tttttaattc agcgatttct
     4141 cttacttgat cgctagaaac tttcgcaact ttatttttgt ttggttcgcc tgaacctttt
     4201 tcgattttag ctgctttttt caatagaaca gcagctggcg gtgtttttgt aacgaatgtg
     4261 aatgaacggt cttcgtatac agaaataaca accggaatga tcaaacctgc ttgatcagct
     4321 gtacgagcgt tgaattcttt tgtgaatccc atgatattga tacccgcttg acctagtgca
     4381 ggacctactg gtggagctgg agttgcttta cctgcaggaa tttgcaattt aacgattttt
     4441 tctactttct ttgccacgag acatacctcc ttaagtccgt gatgtggtta attggagatg
     4501 catatttctc ctcccacgtt ttatctgatc tgaaaaaatc cagacaaata atgtgcaaaa
     4561 caatgcacac tggtacagta tagcaaattt atttgttttt tcaagatttt tttctgctat
     4621 ttttacattt tgcttgaata aacgctaaat ttattctaaa aaaacaacaa tttttcgttc
     4681 tcatttcttc ttttatgtta cacttgcgct agcatggttc aacagatgaa agcaaacaat
     4741 aacacggttt tttagaccct tgtacaaaga ttcctacata acaaaatgag gtggagaaaa
     4801 tgaaagattt aaggtataga agtgtctttg atattattgg acctgtgatg attggtccaa
     4861 gtagctctca cactgccgga gctgctagaa tcgggaaaat catacgtagt atatttggcg
     4921 aacagcctga ttctgtagat atttatctat atgaatcatt tgcaaaaacc tatcgggggc
     4981 acggaacaga tattgcatta gttggcggcc tgttagggat ggaacccgat gatgaacgtt
     5041 tagctgattc tttgaaattg gcatatgaag ccggtatgga agtcttgttt gttcctaaga
     5101 gtgaaaaagc cgaacatcca aactctgtaa agatgcttct aaaaaaagga acacgaaagc
     5161 tttcagtcac tggcatttcg atcggcggtg gcaatatcca gatttctgaa ttgaatggat
     5221 tcaaaatttc tttaagcatg ggtactccta ccttgatcgt tgtccaccaa gatgttccag
     5281 gtatgattgc taaagtgact agtatacttt ctgagaccaa tacgaatatc ggcactatga
     5341 cagtgacacg ggaatcaaaa ggtgaaaatg ccattatgat aattgaagta gatgatcctc
     5401 aagtagaaga aacggtaaaa agactcaaac agctgccaaa tatcgacagc gtaaattatt
     5461 tcgattaagg agctaaaaaa atgtttttat cgattgaaga attagtagat caagcaaaag
     5521 cttatccaag tgtagcagaa ttgatgattg cagttgaaat ggaaatgagt ggccgttccc
     5581 gtagtcacat catcgagatc atggaaaaaa acctctcagt aatggaacaa tcaatcaacg
     5641 agggaaaaga aggtgttacc tccgtcacag gaattaccgg tggtgatgca aaaaaattag
     5701 atgattattt aaatgaggca gactttttaa gtggagaaac aatcttaaaa gctgtacgca
     5761 atgctgtagc tgttaatgaa gtcaatgcaa aaatggggtt gatttgtgct acaccgactg
     5821 ccggaagcgc gggtgttgtt cctggtgtgc tgatggctgt ccgagatcgg cttgatttga
     5881 cacatgaaca gcaattggaa ttcttattta ccgctggcgc tttcgggtta gtcatcgcaa
     5941 acaacgcttc tatcagtggt gcagaagggg gatgtcaagc agaagttggt tctgcaagtg
     6001 caatggcagc agcagctttg gtttgtgcaa aaaatgggac tcctcatcaa gcagcacaag
     6061 cagtagccat tacattgaaa aatatgatgg gactgatttg cgatcccgtt gcaggcttag
     6121 ttgaagtccc gtgtgtcaaa cgtaatgctt taggctcttc tcaagctttt atctctgctg
     6181 atatggcatt agcgggtatc gaaagtgtca ttccgccgga tgaagtcgtt gctgcaatgt
     6241 accaagtcgg acgtcaaatg ccgtcgattt tcaaagaaac agcagaaggt ggcttagctg
     6301 ttacgccaac tgctaaacga cttgcggaag agattttaaa taagagggcg tgataaagct
     6361 ccttaatttc gagtattaag agattagtta tcttcataac aaaaaacgaa gagaggacta
     6421 attgcccttc tcttcgtttt ttattaatcc atcaatttcg tattttttac ttcatcgatc
     6481 gtttttgttc ctgcaagttg catcgtgatc gacaattctt tgttcaggtg ttcaaaaaca
     6541 gatgttactc cttctgcccc acctagattc aacccataaa tcactggtcg gccaatcgca
     6601 actaagtcag caccacttgc cagagctttg aagacatgct ctcctcgacg gacaccagag
     6661 tcgaaaatca ctggtacacg tttgttgact acttcggcaa tttttggcaa tacttcaaag
     6721 gacgcaggac ctccatctaa ttgacgtcca ccatgattgg atacccagat accatctgcc
     6781 cctgcagaga tagcgattac tgcatcttct ggcgattgta ttccttttac gataactgga
     6841 agatgggtga tttctttgat tgttttgatg tcatctggtg tcaagccttg ttttgcagct
     6901 gcataaattt ctgcgattcc ttttccttcg ccattaccac ttgcactttg ttcgctatac
     6961 gcagctaagt taggcattgg taatggaaat tggaattgat tgatgacgtc ttcttcacgg
     7021 taaccaccga gcgttgaatc tgccgttaaa atgatagctt tagcccctgc ttctactgct
     7081 ttgttcaaaa tgaactcatt gaatctgtcg tctttactca tatataattg gaaaaactgc
     7141 ggtgcgcctg gcgctgcatc ggctgcgtct ttgattgtcg tatttgcata agtgctgatg
     7201 gaaaagattg atccagcagc agctactcct ttagctgtat cagcttcccc tttttcatgt
     7261 gccaatcctt gggccgctga tggcgcttgg ataatcggtg tatctaattc aattccgaaa
     7321 acagatgtat gcaaatcggc tgagtcgatt ccgcgtaata tccgtggcat gattttcttt
     7381 gtattaaaag aagtcgtatt ttctttcatc gtccactcat cttcagaacc accgcgaata
     7441 tagccaaaag ctcctgcttc catgcgctct tttacacgac cttctagtta ggcaatgttt
     7501 acaatatcga ttggatgttc ttcattactt gtatgataag tcatagtaga tccttctttc
     7561 atcttcttga ttacatataa atcatactaa acttacaaaa agtaagcaaa catttactct
     7621 cagatacttt tatcgctatt ctttccaaat aaatcgactt acaagctgat ctacttcttg
     7681 gttttcatga agcatactat gagaggcatt ttcacctgtc actacttttt cctctatttc
     7741 atttccgcgt tgcttcaaca acgcatagac cgctaatgca ctgtttaacg gtactgttcc
     7801 atcactcaag tctgtttcat ctagttgacc agcaatcaag aaaaatcgag tggttatagg
     7861 aatattaccg atcatttgct ggtaatcttg atagcggtta gaaacgacag caggcccctt
     7921 tgctaactca tctgtcagag actgcgcact atcatcgaca aaatcattaa aaggcgcgcc
     7981 aattgctgtt aatgtctgaa cttgtggcaa tgacgtatcc tgtccatacg ttcctaaata
     8041 gcgtagtgca ctaactcctc ccatggaatg ccccactatg ttgactttat ctatgccgta
     8101 gttttctttc agatacaaaa gacaagtttt gatccattct gcttgattcc actcgttatt
     8161 tttattatct tcaaacaata cttggaccat cggattgtct gcttttccag acaaatctcc
     8221 tgttgcttgt atttgtccat ctgttcctac tgttaaaacc aattctttta ctgcttgctg
     8281 atttttttcc aaacgccgca acatcccttt aaaggaattt tctgtcccgc tgtagccgtg
     8341 gaaaaaaagt gttggaatag tggttttttc tatcacttta ctggattctg ccgttttttc
     8401 tttatcagaa acttcagtag tatttatctt cgtcgtctgg caaccactta acgcaaacaa
     8461 actgataagg actaaagctg ctgcagttgc acgtttgcat aaatttttga ttggttctgt
     8521 catacacctc ttcctttcca tatagaaaaa agaaagactt gaaaggccaa tcatatgatc
     8581 gacatttcaa gtctctttat taatcgattt tatctacttg atcgaaatca agttctgtac
     8641 tggtttcacg tccaaacata tcgatgttga ctttcaattt ttctttttct ccgtcaactt
     8701 ctgttacttg tccttctaat ccagcaaatg cgccttcgat gattttcacg acttcgccaa
     8761 cttctacttc tagatcattt tgtcgcaggc tcataccgat agaacgcaag atatgattga
     8821 cttcttctgg aagtaatggt gctggtttgc ttccggctcc gtgtgaacca acgaatcctg
     8881 tcacaccagg tgtgttgcgg acaacatacc atgaagcgtc tgtcatgatc atttcaacaa
     8941 gtacgtatcc tgggaatgtt ttatgaacga tttctttttc tttcccattt ttgacttcag
     9001 tttctttttc ttcaggaaca acgacacgga aaataaagtc ggccattttc atactttgcg
     9061 cacgagattc gatatttgct tttactttgt tttcatatcc tgaatatgta tgcaagacat
     9121 accagttttt ttcaaaagat tccattgaag gctcctcatt tcattgtaaa ataaaaaaac
     9181 ctcagtacac ccgaagtttt tctttcgctt cattatagca ctaaaagtac gttgtggcta
     9241 gcgagttatt ttaaaatcca gccaaataag ctttggatgg ctgtatccat tacgtaaaac
     9301 atcaatccga aaataataga agtttcgatg actactaacg tatctttacg gagttgtttt
     9361 ctgcttggcc aagttacttg tttgatttca tcttttacgc tttttagaaa tttcatgaga
     9421 caacctccct tgctactgtt tatttcgttt ctttatgtaa tgtatattga ttacaatatt
     9481 tacaaaactt attgatttcc aaacgttcgc cgctttttcc ttcactgact gattttgtgt
     9541 agttgcgaga cccacaaacg gaacaagcga gtgctgcttt tttctttgcc atttacttct
     9601 gcccccttgc tgagaatcag acttatactt cataacagta tcacgtaact ttttacttgt
     9661 caatttgtga aataaactat ctattgtttt ggataaaatt atgatatgat taatttgaaa
     9721 ttaagctaaa atgagacaat tggaggtata gcgatgctat taaaaacact tgtatataag
     9781 aaaaaagatc tgacaaccgt tacagagact agtacattag aagaagcatt aaaaatttta
     9841 gaaacttccg gataccgttg tgtgccgatc ttagattctt caggtaatat tttccgtgga
     9901 aacatttaca aaatgcatat ctatcgtcat aaagcaaatg gcggtgatat gacgcttcct
     9961 gtcactcatc ttttgaaaaa cgctacaaag ttcatttacc tggatacttc tttcttcaaa
    10021 gtattcttta cgatcaaaga gcttccttat attgctgtcc ttgacagtga aaatcatttt
    10081 tatggtattt tgacacatag tacgttgctt aatatgctct ctcaatcttg gagcgtagaa
    10141 caaggaagct atgtactgac aattgcttcc acaggaaagc aaggagattt ggctgctatt
    10201 gcaaaaatca tttcaaaaca cagcagcatt gccagctgta ttactcttga tatcgaaaaa
    10261 gacgaataca ttcgacgtac attgatcacg ctaccggcta acactgaagc gaaagtatgt
    10321 gaaacgatcg tcaaccactt agagaaaaag aattataaag tggtagaaat tgaaaatttg
    10381 caaaaccaag aagcataatc aaaaagcgaa aaaagaaaac agctaatctg ctttcttttt
    10441 tcgcttttta taattagaca cgcttttcaa aatcaagcgc gcgatctaaa taatacatca
    10501 atggtgctga aacagccatg acgataaact cacttaatgc aagcgtgcca taggttgtcc
    10561 aaaatgcttg gccacctgtt ggaactaaca tataagcaat cagtcccatg ctgatggtaa
    10621 agaagatcgt gttcaatact aaacgcatct ttacattttt cactttgttt tgcaataaag
    10681 ctgtcaggcc taaagaaagc aatgtttggc cgccaccaaa aaccacatcc atgataccaa
    10741 aaccaaagaa ggcattatag acgatgaccc cgcctaaaac tccccaaagc aatttacggt
    10801 taaagacaac taaatgattc aaactttctg aaatacgaaa ctggatcggc cctgaggata
    10861 caggcgcaac taaaatagtt aacgctagat aaagagccat gatgatgcca tttacaacga
    10921 ttacggataa ccgttgtctg cttttcgttt tttgcatttt ctctcctctt ttctcctagt
    10981 ttttttaacg cgggatggtt gatgaaccgc ggttatgtga aacaaacaat aattataaca
    11041 gacttttcat ttatttcaat ccgtcttatc tgcttttaaa aaagcaaaag cttaaacaat
    11101 gtaaacgttt gctaaaaatc gtgggatatg gtataaagta agtgtaaggg gtcataagaa
    11161 ttcccagtaa agcagacgaa gtaggatctg caagaacaga attgttgtga aacttaaata
    11221 aataaaaaag gatgtgggaa tttgagcaac ctcttactag agaacggtct agcaagcagc
    11281 tgaagatcta agtggacatg ccgaaccttg gaacttctcc ttacgtaagt aatggatcat
    11341 gcgcttgcta ggcttttttg tgtttttcgg tcttcggacc tttttcattt tcctagactt
    11401 ctgctataat aaccaagaat taattttgta acagattttt ttgaaaaaac aagtttcttt
    11461 cattcaaaaa atagaaaaag cttgctaatt attggttaac cagcaaacag gagtattaaa
    11521 atatcttgtt ttattgaata gcaggaggaa atcatgaaat tccattatca tatattgatc
    11581 aatccatcgg caggcagcgg aaatggctat aaagtagcag aaagaatttt accggtatta
    11641 aaaaataagc atattgacta tacacttcat tatagtgaat acaaaggaca cgatgctgag
    11701 attgctgaaa ctttggcaaa agaaacattg attccttgga tagaagaaga acagaaaaca
    11761 atcgatactt tccccctttt gatcatcgta ggaggtgacg gcaccttaca tcaggtactt
    11821 gatactttct atcagatgga agtagaattc ccagttgctt atattccagc aggttctgga
    11881 aatgattttg ctcgaggggc aggccttcca aaaaatccca aaaaaggact tcagctgatt
    11941 ttagcagctc aatcaccaga aaaagtccac ataatggctt atgaagaaaa aataagtgaa
    12001 aaaaaaggaa tagctgttaa taatttcggt ataggattag atgcagctat cgtccatgcg
    12061 acaaatcatt cttccacaaa aaagcgattg aacaaataca acttaggatc attttcgtat
    12121 ttattctcta ttctccgtgc tttattcact caaaaaggat ttccaattct ggtagatgca
    12181 ggaggaaaac aattaagttt ttcaaacgca tttttatgta cagcaacgaa gcatcctttt
    12241 tttggcggag gaatcgcgat tgttccaact gctgatgcgt ctaaaccagt cattgatttt
    12301 gtcgtggtcg aacgaatcaa tttctttaaa atcttgtggc tcattctttt acttatacag
    12361 aaaaaacaat tgaaatcgaa atatttccat cactacacaa caaacagatt acgtatcgtt
    12421 tcaacaacac cgcaacatgg tcaagaagat ggagaagaaa tggaaaaacg accttttgat
    12481 atcacaataa ccacaactaa acagttgttt tggtgttaat aaaagaggtt ttgtcagaag
    12541 ctacatctgg aacaccgttt attcggaaaa aaggactgtg acacgacttt tgtcacagtc
    12601 ctttttttac tatgcagtta attctttgcc caattgggtt gccatcgata gtttctttct
    12661 ttacccaaaa taaattggat cattgaaaac accgcagcaa aagcaactaa gacacaacca
    12721 ttgaaccaaa aagattctgc aaatgtttga ttgtaactga gtgtttcact gatcaagtta
    12781 ttattttgaa caaaccaata tgttggtgtg aatgccgcta ttttgctaac catttctggc
    12841 aagtattctg ctggtacaaa caccccaccg ataaagcaac tgcctgtgat aaaaatatct
    12901 ttgatcccac cgctggtttc tgagtttttt accaatgaag tgatacaagc cgaaaagcta
    12961 atgatcggca ataaaaacaa gaaagtattt aaaatatgat accataccgt tatatcccac
    13021 gaggtgtgga tcactaacag aaccattaga agtaagaagc cactgaacaa cagcagcgag
    13081 taactaatca aactaaaggt tactttgcga ttcaagtttc gtttggtaat gggcgcactc
    13141 tggtttcgtt tactgatctc tttgcgatta aacgctagat tgatgacgct gaaaccgcta
    13201 aaaatggata gaaacaaccc ataagacaag aaattaaagg ttgtgcccgt caacagcttt
    13261 ttcatccgct gactatatcc cgcacttagc tcaacttctc cagtaaccga gagtgccatt
    13321 tgtgtctgtt ccatagctgc atcgattgtt ttatcttctt cttgaaacag aatcagtgtt
    13381 tgcaaaaagg tgttcacttg ctgtgtaaca actgttttag aaaaggcatc tgggcgactc
    13441 tgtatcttga tcgtcgccat ctttcctact tcgacttgtt cggtaaagtc tgcaggcaga
    13501 tgtaagataa tgtttacacg attaaaataa agagcatcat cgatttcttt ttgtgaggtt
    13561 ccatccaatg taacgactgt ttccttgtca ttcaaatatt tacgaaggcc atcgactaca
    13621 ggcgactctt cttcactaat caaggcgatt ttcgcatctt gcagagaggt attttcaaca
    13681 tcaatatttt gcgcaaaaaa gaatgtggca acaacagtga taattacacc tatcagtaaa
    13741 ttcgatttat ttgctttaag caatgtccaa atcgttttat aaagcgtcat aacgcacccc
    13801 tctttccatc caagcatttc ctaacgcaaa aaggaaaata aatccgatca accatagaag
    13861 attggtataa aaactagcca aatcagtgta gaaataaagt ttatacaaac tttcgctgat
    13921 caagttgacg agatttaatt gaccaacaat cggaatatga agatcaatcc aatacttcac
    13981 ttgttccgac cccatcatac cagcaaaaaa gctcatcgca atcgtcactg aaatcaagat
    14041 gctatccttt tgcaccagat tcatttttgg tatcaaatta ccaaccaatg tacctaacaa
    14101 aatcgcacac aacgacccca gagcggtaac aatcatcaac cattgccaac gttcaccaaa
    14161 gtctacttga tagataaaac gaaagacagc taacataata taaatctctg tcaaaacaat
    14221 cgtaaagctt gctgccaaat tagcgattga aactaacgat tgctttctag gaatcaagga
    14281 caagcgaata ccatttgacg attgatttgc ttgctgatcc tgtacattgc ggacccccca
    14341 catcgtcccg tagagaatcg ccattccaat cagagtgaag aaataaaaac ttttgaaact
    14401 catattacga cctgcatgaa cttcttgaaa agatacttct tgttcaaaaa cagtcatgat
    14461 gggcgcgaga tcaacgttct ccacttctgc cactttactc gaaattatac caatacgctg
    14521 taaaaattga tccatgacgt ttttcatcaa cgtctgactc attcctgctt gacccacatg
    14581 gagcgaaaca gatgaaccat caatgagata aaaaccagcc aaatcatctg catttagctt
    14641 ttcattcgct tctacttccg atagttcctg tacttcaaat aatggtttat cattgacttc
    14701 gacttgttct aagactgtgg taaaattttc cgaaagcgct gtttgatccg ctttgattac
    14761 aattccagta ttaatcgttt caagattgcc accttgatca atctcgccaa aggccgccat
    14821 aaacatcaat cctaaaagaa ttggaaaggc caatgtccaa aacaacatgg atttatttcg
    14881 cagcagtatt tttgtgcgat atttataaaa atgccaaaac attatttccc ctcctctagt
    14941 ctctcaaatc cttgccagta atctctaaaa agacatcatt caaggacgct tctttggttg
    15001 ctatttttcc gtaactgatc ttttcttctt gtaagattgt aagaatcggc aacaatactg
    15061 tatttagttc agtagttgtt acttggagct gacctttttt ataagcgatt ccatcagaag
    15121 ctattgttcg ctgaatcgct gacaacacat tttctgggag taatgggatg tcgatcgtca
    15181 aacgatccgc cgtccgtacc atatctttta gagattcttt ggttccttta gcaatcacct
    15241 gtccttgatc catgatcacg atctggtcgc aaagaatttc tacttcttcc atgtaatgtg
    15301 ttgtatagac aatggttgct ccttgtcgat tcagctcctt gatactttct aaaattttat
    15361 tccgactttg ggggtctaca gctactgtcg gctcatctaa aaaaattaac cgcggcttgt
    15421 gggcaatccc acatgcgatg ttcaaccgac gtttcaagcc accactaagt tctcctggac
    15481 gaaacgaaac atatttttct aatcccacta attgaatcac ctcttcaatc aaggctttcc
    15541 ttttctcttt ttcggaaaca tataaaccgc aaaaataatc gatattttct tgcacactta
    15601 gttcgtcaaa gaccgcaacg tcttgaggaa cgaccccaat cattgccttg acagcataat
    15661 tttcaggagt cattgttgtc ccgaacaatt taatggttcc tttgtcataa tttaacaaag
    15721 aaagcataca gttgatggca gtcgatttcc cactgccgtt tggacctaac agacctaaga
    15781 cttctccttc ttgtacttgc agatcaaaat gattcaatgc tacaaaatct ccatagcgtt
    15841 tgactaattt atcaatttcg ataatcatca tgaaaaactc cttccaaatt tcttgttgat
    15901 ctgactataa taaagtgcaa gaaaagaaac tagtgacgat tggctaaacc acacatgaca
    15961 tttgtcacaa tgaaggagtc tgctatgcat aaatctcggc tactcaacga aactagcttt
    16021 ctactggtta tctcattgct tttgctggta agtcaaggat tcgttgctat gcaagttatc
    16081 tatttattga tcgccgtttt attcactggt gtcagttatt tactgcctac aaagccaaat
    16141 tggattttcc aagttattct atttctttta tgtgcctttt tacctgatct tgtcctgttt
    16201 ctgccagcga cgatgcgagt gatcgcccct gaagaaagcc accttgatta cttgtttttg
    16261 ttaggttttt tgttgatttc gacggttggt aatcatcccc ccatcaaggt cgcctttgtc
    16321 gtaattttgc tgtttttttc cttttactta cgaaaaaaag atcaacgaac gaccgatctt
    16381 gagaaaaagt acattgtctc caaagatact cattgggaaa aacaacaaga gttatcggaa
    16441 caaaataaag cattggccct gtcacaagaa acacaattag ccttgcaaat tgtggaagaa
    16501 cggaaccgaa ttgcccgaga catccatgac aatgtcggtc atctgctgtc aagtgcgatt
    16561 ttacaaattg gtgcgcttga aatgatcaat caagaaccaa aactaaaaga accattagaa
    16621 aatttaagtg ccaccgttca cactgggatg gatcggattc gtcaaagtgt ccatgacctc
    16681 catgagacat cgatctcttt tcaacactcc ttagagtttc tgatcgctga ttttccttct
    16741 tctgttacga ttgaaggaaa cttgtttgaa ggattgaatg aaacgcagca aaactgcttc
    16801 ttaatggtca tcaaagaagc cttaaccaac agtatcaaac atagtcatgc caaaaccgtt
    16861 gttttacatt ttcgaacgtt gccggccttt tatcgcttgc aaatcacgaa tgatggcacc
    16921 agcccacaaa aaaattctgc tcccggtatt ggtttgaaaa cgatgcgtca acgtgttcaa
    16981 gaacaaaagg gacaagtcca tattttccaa gaaaaccagc aatttcagtt aacgatcatt
    17041 ttaccaaggg agggacacca atgataacag tagtcattat tgatgatgat ccatttgtga
    17101 cgaaatcgtt acaaaccatt ttagaaagca cgaaagagat ccgtgtgtta ggcattggtc
    17161 attgtgccaa ggatgcgcta gtcttatacg aaacccatcg tcccgatgtt ttgctgacgg
    17221 acatccgcat gcctaaacaa acgggcatcg atgctgccaa agaaattttg gctaaatttc
    17281 ccaaagccat ccttttgctt ttgacgacat tcaaagatga agaatacatc cgcgaagcat
    17341 tttccatcgg tgtcaaaggg tatttaatca aacagaactt gcaagcgatt attccttctg
    17401 tcaaatccgc ttataatgga caggccgtat ttggtaatga aatcatcgag actttcactc
    17461 aaatgatgaa gaataaaccg acgattgata gtcatgctta tgcttctttt agtgaacgtg
    17521 agcttgcaat catcaaagaa gtagctgccg ggaaaaacaa taaagaaatt gcagatgcct
    17581 tgtacttgag tgacggcact gttcgcaatt acatcagcca attactagaa aagctagatt
    17641 tacgggatcg cacgcaattg gctatctatt tttatcagca tttgcaataa gtcttatcta
    17701 acacaaaaaa acctgaatga tgctccttcg catcattcag gtttttttca catcttctat
    17761 tcaattgtcc cgcaaaccat ttcctcacga ttgaaagaat caaggattcg cagggctgcc
    17821 aggattcgtt ggattgttcg gcgtgttagg cggaaaaata ggcgccttgt ctaacgtttt
    17881 ttctagtttt gattcttcac gatcgtctcg ttctacatca ggactttcat tcaatttgaa
    17941 ttctttttcc atctcatcta actttttaaa gtcattgcca gattcttttg ctaatttcgc
    18001 taatttatct tcggtttctt tagccataaa gtaacgcccc ccttttgatt tcagcttagc
    18061 atatcacact gaaaaaacaa aagaaaacgc taaccaacga tcatttttag ctatttctca
    18121 tctttaaaat caagcatttt ttgccggtat aaaatcgtag ggagaactgt tttccatacc
    18181 aatcatcgga tctactaact ggttatcgat cacttctgct gtaagaacga aatctttaga
    18241 aacagcaggt tctacctctt ctttcttttc ttctccaaca aatgaagaaa acagatctgg
    18301 ttccactgtt tccattactt ggtttttcgt ttcattcgtc ccactgtcat cttctacagg
    18361 tagcttttct tttacaaccc gtggctcttt tgtatagttc ccatcttcaa atggatcatt
    18421 tccgaccaaa gaatcttcat aagctgctag tttggtccgg agagtgatat tcattttatc
    18481 attatccatg atccttgatt tcaacgtagt catccggctt gctgattcat tcgctacaca
    18541 agtgacatag gcttgctctt cccctaacaa aatcaacagt tccttactgc gagtcactgc
    18601 cgtgtagagc aaattacgct gaagcatccg ctgatattga tggaccatgg gcaaaataac
    18661 catcttgaat tcactgcctt gtgatttatg gattgagcaa caataagaaa gagtgatttt
    18721 attccattcg tttcttttat aagtgacctc atttgcatca aactggataa ctaattcatc
    18781 cactttatct tcagaatctt ttgccaagat gatacccgtg atttctccca tatctccatt
    18841 gaatacattc aattcgggag tattgactaa ttggaggact ttgtctccta tccgataaac
    18901 ggtgtcgttc caatgtactt ccttttttcc aggtttccct ggattgaata tctcctgcat
    18961 cattttattt aacgcatcga tccctgcagg tccccgatac attggtgcta atacttggat
    19021 atcttgtgca cggaatccct tatttttcgc tttttccaca attttagaga tcaagggttc
    19081 aatttgataa gcatcacttc ggaaatagga acgatctttt tgattctttg tgaaatcttt
    19141 tggcagacgt ccttctttga tctcatgggc aagaggaata atactagaac catcaccttg
    19201 ccgataaatc tgattgagtt ccattttggg aatttgttcg atttccaaaa gatcatggag
    19261 tacttgacca ggaccgacag agggaagctg atctttgtct cctactaaga taacttgcat
    19321 attcgtcgga atcgctttga ataatgtgtt agccagccat gtatcgacca tagacatctc
    19381 atcaacaatc aaaagtccgc cttctaattc tttcgtactg actgaaggtg ttttttcccg
    19441 tccattcagc cccagaagac gatggatcgt actggcaggc agacctgtcg tttcgttcat
    19501 tcgtttagct gctcggccag ttggtgctgc aagtagaata ggaaaaattt cttgggtata
    19561 ttttgaagga tccaattcca gtccgttcaa ctctgcaaaa agattgacga ttccgttgat
    19621 cacagtagtt ttccccgtac ctggtcctcc tgttaaaata aatagagggg aacggattgc
    19681 ttcagcgata gcttgttctt gcgaaggtcc atactgtatg ccgaatattt tttcgatacg
    19741 ccgaatattc ttctgcactt ctttttcagg ataatggatt tcttttttac gactgagaag
    19801 gcgctggatc gaattaccga tcccccattc agaaaagtac aaactgtttt cgtaaagatt
    19861 agtctcttct tgttgtatct ttccttcttc aacaagttgg ataattacag tagcaacctg
    19921 atcaggctgg atttcaattg ggcgacttgt ttctaaaata cggatcgttt gctgaagcaa
    19981 tgtctccgcc ggtacgtatg tatctcctgt ttgaactgca tgctgaaaaa tctgatgcaa
    20041 gatagctgct cgaattcttc tcggtgaatc tgctgcaata ccgatttgtt cggctagatt
    20101 atctgcacgt ttgaacccga ttccttctat gtcttcgact agctggtacg gattttcttc
    20161 gatgatatcc aaagactcgt tcttataggc ctggtagata ctgaaagcta actgactacc
    20221 gaacccgtaa cgattcagtc ccatgatcac ttgatccatc ccgtggttta gccgaatcgt
    20281 ctcagcaagc atttctcttt ttggcttcgt caaaccggag atttgtttta ataccgaagg
    20341 atcagcaatg attttttcga tagcatcttc tcccagcaat tctacaattt tttctgctgt
    20401 tttcttcccg attcctggga atttttcgct ggaaagatac gctacgatcc catttgcagt
    20461 tgttggcttt tcttgctcat atctttcgag tttaagctgc cttccgtatt taggatgatc
    20521 cactaattct ccataaaaac gataagtctc attttcttga atatcgccga aacttccggt
    20581 gactacaatc tctttttcac ggtaatcagc tgtggtttca ctgatgtgta ccaaaagcac
    20641 tttgtaaaaa ttactaggat tttgaaaaaa aatcgcggca actgtaccaa cgaaataagg
    20701 catttcttct ggctgcatcg gaagccgcct ccttcttcta tagtaaaggt tctggtgctg
    20761 tttcagttcc taaaaaatct gtttggttcc atactttcag ttcatatggc aataatctgc
    20821 aaggctcttc agtctctaaa atcgtcaaac tactattgaa caatccaccc atttcacgca
    20881 actcacttag ctcttttcct gtcatccatt gaatggctgc agttaaagaa gctccatgtc
    20941 ctacgaataa aagtgggcca tctttatgtc gggcaaccgc atctgctaca acagtttcga
    21001 tacgtgtgat tgccgattca atcgtttctc cgtgaaaagg agaaggatca tacagatcta
    21061 aatgattacg caaattcggc agattatccg gatatttctg gtacatttca tcgatcgact
    21121 gaccttctaa agtacccagc cctagttctc ttagcttatc tgtataaaaa atctctaccg
    21181 gacgagcaag ctctttgttg ataccttctg ctgtatcacg tgctcgcttt gctgtactgg
    21241 aataaattgc tttaaaagga acatatttta cctttttccc aaatgctttg atttcttcgt
    21301 aactttgtgg caaaagcgga gaatcgccat tgctcccttg aaaacgtctt tcctgattcc
    21361 attgtgtctt cccatgtcgg gtaaaatata gttccatacg cagcaaactc ctgtctttct
    21421 tttattttac tctataataa actgtctaat ccagcttgtc attttcatag tgatgcgtca
    21481 attctaaatt agggaatcct tgctggcgaa gtgcttcata cactatcatt gcagctgtat
    21541 tcgataaatt caatgagcgg acatgctcat cattcatagg gatgcgtaaa cacttttctt
    21601 cgttttcacg cataaatgtt tctggcaaac cggttgtttc ttttccaaat aaaaagaagt
    21661 gctcttctcc atcagcaaaa ttttcgtcac tatatacttt atgggcaaat tttgagatca
    21721 aatgtagttt acgattacct aaaaatgtta aaaaagcttc taaattactg tggtaagtaa
    21781 tatttacatc attccaataa tccaaccctg cccgttttag atgtttgtca tcagttgaaa
    21841 aacctagtgg ctcgattaaa tgaagcggag tattcgtggc tgcacacgta cgggcaatat
    21901 ttccagtgtt tgcagggatt aatggttcaa ataaaacgat atgattcatc aggatcattc
    21961 ctctcatatt tatgttcgtt tctattttct gacaatttga tttttgcata aaaaaaaacg
    22021 tatcaactcg gtgtcagtca ctgcgagtcg ttacgttagt gaagctcttc tcactaactt
    22081 ttatcagaac aggaacctga taaaaaccaa tgaaaaaaca acttgttgct aatataacac
    22141 cctcaacttt tttttgcaac ctctactcat taaaaactgc tagaaaaacg ttttctctta
    22201 gttcctttta ataattctat gtcaattgta atcttcttca aaggattctt atataagtct
    22261 gctttgattc gctcttctac ctgccattct aaaggtgaca tggccaaaag atctttttga
    22321 ttttcctctg gtatctcaaa ttcataggtt acacgctgac gttcagttaa tttcattgct
    22381 tcagcaaact tggctactac acgttcattg gaataactct gtttttcagg ttgattcgga
    22441 taaaaagcct gacgtagttc ctttaaataa ttttcttgtg gcaccacctt caacacttct
    22501 ccatcttctg agagtacacg ttggaattct tggtaatgag aaggcgaaaa gatatttaaa
    22561 accgcgttga atgtttgatc agcaaacggc aaattggtca aatcagctac acaccaaaat
    22621 gcttcggcag attgttctgt agctgcgtag actccttctt ttgaaatatc aaatccaaca
    22681 gcattttgaa tctttttcaa ttttgaaagt tcattcaaga agctaccttc tccgcagcca
    22741 acatctaata gcgagacttc ttcaggtaaa tgctgcgcaa tagtctcaat aacgggctga
    22801 tacatgccag attggatcat tctacgcctt ggaacgaaca ttccttgatc gtattctgtt
    22861 ttaaccgcat gggataaaaa ataaagtgtt ccttttttgg aaaggtcaaa acggtgatta
    22921 tttttgcata tcaagccatt atcagacttt tttacttctt cttggcaaac cggacagcgg
    22981 aacagctgat gattttctgc caaaaactgg cgtcctcggt caattttttt taacatagtt
    23041 ccccctcatt tctgttttat cttaccgtta gatgctcact ttggcaactg ctaaaaatat
    23101 gatacaatgc ctagaagaaa gtgaaagata aaggagaaga ttagatgatc aaagaatttt
    23161 gcgctgaaaa ctacacatct attcctctag caattgctag aggagccgat cggatcgaat
    23221 tgtgtgacaa cttagcggtc ggtggcacaa ccccgagtac tggtgtgatc gaagaaacgt
    23281 taagttatgc tggggaaaaa gatatccccg taatgacgat tattcgtcct agagggggcg
    23341 acttcgtcta taacgatatc gaattgaaaa tcatgcatac tgatttgatt gaagcaaaga
    23401 aattagggac ggatggcgta gtatttggtt gtcttactcc ttcaggctgg ctagacgaag
    23461 aggccttaga actattgatc gaaacagcag aagggttgca gatcaccttc catatggctt
    23521 ttgacagtat tcctgaagat cggcaattcg aagcaatcga ttggctggta gaacatggtg
    23581 ttgaccgtat cttgactcat ggcggtcctg ctgacaaaac tattgtagaa aatttcccac
    23641 ggcttaaaga attgattgcc tatacagatg gccgcttaat cattttgcct ggtggtggtg
    23701 tcactgcaga gaatgcagca catgtagcta aagaattgaa cgtaagcgaa gtacacggaa
    23761 ccaaaatcgt ggatcttcag ttataatctt ataaacaaaa aaacatttgc aacaggcata
    23821 atgcttattg caaatgtttt tgttttttta cataaaatag cccataaata aagaagcaat
    23881 attgaaataa atcagtacac tcgtcaaatc gctcaaagtc gtgataaagg gaccgcttgc
    23941 tactgctgga tcaaatccta gacgatccat tagcatcggg attaaactac ctgcaagatt
    24001 cgccactgtg attgcaaata gcatagccat ccctatgaca aaccccaaga taaaattatg
    24061 tttccaaaaa cctacgatta gaaaaatcgt gatccccgta acagctcctg tgacaacgcc
    24121 ggtcaagact tcagccaaaa caagtcgaac aaatccatga tctttctcat cagaaatcgc
    24181 aagtcgacga acagccacag ctagcgactg ggttccagca ttaccggcag ttcctgtaat
    24241 cagcgaaatg aagactgcta aaatactcgc ttcacttacc aagtcttcat agtgactgat
    24301 taaagaagcg gtcgacatac ctaaaaacaa cagagtgatc agccaaggca aacgtcgaga
    24361 cgctgccttt actggatttt cattaatttc ttccacattg accccggcaa gtcctgaata
    24421 atcgctagca gcttcatcat caataacgtc gatgatatca tcgactgtga cgattcctaa
    24481 caaatgatca tcataatcgg taacaggaag agctaagaag tcataatctc taaatgtttg
    24541 tgccacatct tcttggtcgt cgcctacatg aactgaaagc actcgttcgc tcatcagatc
    24601 agaaatcatt gcgtcgtcat cattcacgat taggtcgcgc agagaaataa cccctactag
    24661 ctgaccttct tgattaacga catagatgta atagattgtt tcagctacat ctgcttcgtt
    24721 cttcagcacg tacatcgcag agcgaacagt ttgattggct acgatcgaaa caaattcagt
    24781 tgtcatgatc gcgccagccg tttcatcctc gtaatggaga agttccttga tttcactggc
    24841 atcgtctgcc gacatcaaac ttaaatattt agcaatttgc tttttatcca gcgtattaag
    24901 caagtcaaca gcattatctg tatacatctc agaaagcata tcagctgcat agctaggacg
    24961 catttcatct aggtattctt tcatatgttc gttgtcttct tcaatcacat cgaacatatc
    25021 cgctaattct ttaggcgaca gataggaata gatcagctgc ctgttctctt ctgtaagaga
    25081 ttggtagaat tgcccctgct catagatatg catctctaaa aaactgtttc tgaatgcctg
    25141 aatattattt tcccttaaat cttgagtcaa ctgggcaaat ctttcctcta attcttgatt
    25201 ttcatccaaa ccaatctcac ctcgccttat tcttcaaacc gtctcacaac tgcctccatg
    25261 tctgcggcta aaggctgctt gaactccagc aactcttctg taaatggatg gtaaaaacgc
    25321 agatcataac aatgaagggc ttgtcttttt attcccagat ccattcgccc gccatacata
    25381 tcatcaccga gcagggcaca gccaattgcg gaaaaatgaa ctcttatttg atgtgtcctt
    25441 cctgtatgca gctggatatc aactaacgcg atttctgtta gtcgcttctc tatccagtac
    25501 tcagtctgtg ccgaacgtcc ttcatcagag accattcgct tcaatagcga agaataatct
    25561 cgggcgatcg gcaaatcgat atgtccatgt gccgataaat ctgaaagcct tccactgact
    25621 aaggcctgat attttttcac aaacttcttt tcacgaagtt gcatatctaa ttttgcgtgc
    25681 gcaaatccat gctttgcaaa aagcatcaag ccagatgtat cccgatctag tcgcgtcacc
    25741 acatggatta cttggttttc gtaaccttgt tttttataat aagcctttac acgattagcc
    25801 attgtcccat ttggatggta ttgggcagga atagacgaaa cgcctgctgg tttatttact
    25861 accagaacgt gttcatcttc atacacgata tcgatcggtg tttcatccaa aagtacggtt
    25921 tcatgttctc cttcagcagg aatcacaatg gttaccttat catccttagc tagagaaaac
    25981 aagacatttt ctacttgatc attaactttg atttgaccgc cttgaaattt tattttcgct
    26041 aacagaccct ttgagatccc tttttctttt aaaaaatatt ttacttgctg agaatgatct
    26101 ttctcataat cccatgtaaa ctgcatatca atcctcacta ataaaggcat ctttcacacg
    26161 atgccaaaaa tgcatatgtc gataagaagc aaaatggatc cgttcttctg ctattcgata
    26221 ataaattgag cggatatttg cttttgcgac attcaattga tcaaccgtta caagataatc
    26281 ttctgtattt tccaatttta tttctagcca ttcatcatgg gcaatcacga tcggagaacc
    26341 caaagtacga aacactcgat tattcagtga agcaatctct gccaactgaa aggcattgat
    26401 actaggatgg atcacagcac cgccgacact tttattatat gctgtcgaac cagtaggcgt
    26461 agaaatcgtc aatccatctc ctctaaaact ttcaaacagc tcgtttttga tgtatacatc
    26521 ggctaccatc gtacgattgg cccgtttaat cgttgattca ttcagtgcta aaaaatgctg
    26581 gtccggtgtt tcatccaaat aactgatccg aacatccagc aagggataac taacactttg
    26641 ttcccggttc gttcgtaagc tgtctactag ttcttccagc tcgtaatccc gccaatccgt
    26701 gtaaaaccct aaatgacctg tatgcacgcc caagaaacgc acatcattca gacaatgact
    26761 gtagtgatgg aacgccgaca ataatgtgcc gtctccgccg actgagatga ccaattctgg
    26821 ctcatgatca tctcgttgaa tccctgcttg ttctaaaagt gcgttcaaac gttctgtcac
    26881 ttctatagtc tttgcttctt tattatgaac gatcgctact ttcatcccct gcctcctctc
    26941 tttcgctttc tttcttcaaa agcgttcgat agtagacatc atctgatttt cctcttccat
    27001 gggagaataa ccgctgtgct tcttggattt cttcacggat actggacatc tcttcatcca
    27061 actgataggc agcttctgcc gcacgctgaa gtcgttcact catttcttct ggaaaaacgc
    27121 cttgatattt ataattcagc gaatgctcaa tcgttgccca gaaattcatc gcaagagtcc
    27181 gaatctggat ttccgccaaa attttcttct caccgctgat cagctgtaca ggatactcga
    27241 tcaccagatg ataagaacgg tagccacttg cttttttatt tgtgatgtaa tctcgttctt
    27301 ggagaacttt catgtctttt cttttacgga tgatctcaac aacttgatgg atatcttcta
    27361 caaactgaca catgatcctt aacccggcaa tatcagacat ttcttcttct aaacgtccca
    27421 ttggaatatg ccgtaattgt gattttgtta caatactatc gactggcttg actctcccag
    27481 tgacaaattc aatcgggaca tgtcgatttt gttcacggaa ctgtttgcgg atcccccgca
    27541 gtttcacttt caattctgat acagtttgtt catatggtgc taaaaatgct tcccaatctc
    27601 gttccatagc tgatacctct acttcattaa cattcattgt ttattctacc atatatgcaa
    27661 agttttttcg tactcttgat ttatgcattg accaaaaaaa gaaaattagg caaaatagac
    27721 aaaaaggagt cgatcaaatg acacaaatgc ttgaaatcga atataaatct atgctgacaa
    27781 agcaagagta cgagatattc atcgctcact atcaattaac agataaagat ttccgtgtac
    27841 agactaatat ttatttcgat accgttgatg aacagctgaa gaaacaaaat tgtggcctac
    27901 ggatccgttt cgttggtcat caagctgaat atacattaaa aacacctgct gaaaaagggc
    27961 gtctcgaaac aacggatacc tttataacag aagaagcaga agcatttatc ttcgaaaaac
    28021 gtctgcctcg ttcaggcgca gtttttcaaa aactttctga tttgaatatc gatcctgctt
    28081 cattaatcca aaccggaaag cttacaacaa aacgagctga attccctatt gaagaaggac
    28141 ttttagctat tgatgaaagc tggggagaca atctacacga ttttgaactg gaactagagg
    28201 taggggatgc atctgtggga aaaattgctt ttataaattt tttaaaacgt ttttctttac
    28261 cttatcgtcc tgctaagaac aagatccaac gtatgcttga agctaaaaat tagaaacctt
    28321 tcattttcac aaggcacttg agttcatttt tttacttttt tctgttaagt tataggatgt
    28381 gaaagaatct agtcactagt tgtatagaat ctattttggg ggagattcat atgatcgaaa
    28441 tctatttatt tgttaatcct ttaggggaat actgtttcga aatggaacaa cagttattac
    28501 aatttatcga agaagagtac gggacaccat caaaagaaaa gatgcaattt cgctttcttc
    28561 ctttggtcaa tttgcagaca attggggatg tcatgcaaca aaaaggaatt tctcagaaca
    28621 atcttgagga acgtaatcac ttattctcaa tgacttattc tgcagcattg gactgcaaag
    28681 cggcgcagtt tcaaggaaag aaaaaaggcc gccgtttttt gatcaaattg caagaagcag
    28741 tcggctgtaa tgacatacca tattctcaag aacttgctga atccatattt gaagaaatcg
    28801 gcggcgattt gaatatgttt aaagaagatc gtcagtctga cttcgttaaa gaagtattct
    28861 tttctgatca aaaaattgct cgggaaatgg gcatcacaaa acacccttct gcagtcgttt
    28921 acaattatgc ctgcgaccgt gattttggtg ttttagtaga agtaaaaggt acaagtagcc
    28981 tttggagaat cacagagctg tgtaaaacaa cagataactc atatcaagtt tttaatggtg
    29041 aacatttaca tacaaatgat tctcagttcc attcaagaac tagccattta cacttgcttt
    29101 ctaattaaaa aaggctcttt gtcaaatgat gttggtaaag agaaatctta gaataatttg
    29161 gacagaacag a
//
LOCUS       DACEXQ010000015        28960 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.015, whole genome shotgun sequence.
ACCESSION   DACEXQ010000015 DACEXQ010000000
VERSION     DACEXQ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28960
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.015"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            88..3705
                     /gene="rpoB"
                     /locus_tag="IXH04_000491"
     CDS             88..3705
                     /gene="rpoB"
                     /locus_tag="IXH04_000491"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ1851125.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            3784..7437
                     /gene="rpoC"
                     /locus_tag="IXH04_000492"
     CDS             3784..7437
                     /gene="rpoC"
                     /locus_tag="IXH04_000492"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAQ1851126.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(8032..9057)
                     /locus_tag="IXH04_000493"
     CDS             complement(8032..9057)
                     /locus_tag="IXH04_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1851127.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            9332..11017
                     /locus_tag="IXH04_000494"
     CDS             9332..11017
                     /locus_tag="IXH04_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAQ1851128.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            11031..12314
                     /locus_tag="IXH04_000495"
     CDS             11031..12314
                     /locus_tag="IXH04_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ1851129.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            12384..13253
                     /locus_tag="IXH04_000496"
     CDS             12384..13253
                     /locus_tag="IXH04_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1851130.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            13250..14086
                     /locus_tag="IXH04_000497"
     CDS             13250..14086
                     /locus_tag="IXH04_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1851131.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            14112..15179
                     /locus_tag="IXH04_000498"
     CDS             14112..15179
                     /locus_tag="IXH04_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAQ1851132.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            15176..16003
                     /locus_tag="IXH04_000499"
     CDS             15176..16003
                     /locus_tag="IXH04_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ1851133.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            16048..17073
                     /locus_tag="IXH04_000500"
     CDS             16048..17073
                     /locus_tag="IXH04_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1851134.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            17070..19253
                     /locus_tag="IXH04_000501"
     CDS             17070..19253
                     /locus_tag="IXH04_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ1851135.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIAPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            19253..20383
                     /locus_tag="IXH04_000502"
     CDS             19253..20383
                     /locus_tag="IXH04_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ1851136.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            20380..21057
                     /gene="pgmB"
                     /locus_tag="IXH04_000503"
     CDS             20380..21057
                     /gene="pgmB"
                     /locus_tag="IXH04_000503"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ1851137.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            21141..21668
                     /gene="msrA"
                     /locus_tag="IXH04_000504"
     CDS             21141..21668
                     /gene="msrA"
                     /locus_tag="IXH04_000504"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ1851138.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(21869..22555)
                     /locus_tag="IXH04_000505"
     CDS             complement(21869..22555)
                     /locus_tag="IXH04_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAQ1851139.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            22635..24173
                     /locus_tag="IXH04_000506"
     CDS             22635..24173
                     /locus_tag="IXH04_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAQ1851140.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            24293..24760
                     /locus_tag="IXH04_000507"
     CDS             24293..24760
                     /locus_tag="IXH04_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAQ1851141.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            25279..25722
                     /gene="rplM"
                     /locus_tag="IXH04_000508"
     CDS             25279..25722
                     /gene="rplM"
                     /locus_tag="IXH04_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAQ1851142.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            25738..26130
                     /gene="rpsI"
                     /locus_tag="IXH04_000509"
     CDS             25738..26130
                     /gene="rpsI"
                     /locus_tag="IXH04_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAQ1851143.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(26223..27443)
                     /locus_tag="IXH04_000510"
     CDS             complement(26223..27443)
                     /locus_tag="IXH04_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1851144.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGITSEKYQKVFPTRQLAIKA
                     ENAMKRKIETVLREENANSLELKGKIKFKEFYESKWLPRYELGQTTRSKRAPSYVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQCITAPKKIALEERRIREGNQALSSEELIAWIEAVNTDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFEKQTVRLKHALDKYQKKKFTKGRKDTV
                     IQVPEIVMALLSKWKSVQAEQLLRLKINQTPDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPELAHLSPHKLRHTYATIARQGGANMNQISNALTHSDISTTKIYVNTPDVV
                     DKTVFEAFQKGLKN"
     gene            complement(27515..27673)
                     /locus_tag="IXH04_000511"
     CDS             complement(27515..27673)
                     /locus_tag="IXH04_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1851145.1"
                     /translation="MNKKQTCEFLGIDNNTLDKWIRIYNFPVIRIDGIVRFDIEDIKD
                     FMNTFKTI"
     gene            28361..>28960
                     /locus_tag="IXH04_000512"
     CDS             28361..>28960
                     /locus_tag="IXH04_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ1851146.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYF"
BASE COUNT         9340 a         5309 c         6169 g         8142 t
ORIGIN      
        1 gttgttacct aaaaataata tttgaaatat tattgtaaca attgatcggg tagtcttttt
       61 gaatgacatt ttagaggggt gaagagcttg gctggacacg tagtaaaata cggaaaacat
      121 cgcgaacgta gaagtttcgc aagaatcagt gaagtgttgg aattaccaaa tttgatcgaa
      181 attcaaacag actcttacca atggttctta gatgagggac ttagagaaat gtttgaagac
      241 attttgccaa tcgatgactt caatggcaat ctatctttgg aatttgtaga ttatgaattg
      301 aaagaaccaa agtatacagt agcagaagct cgtgcgcatg atgcaaacta ctctgcacca
      361 ttacatgtaa cattgcgttt gacaaaccgc gagactggcg aaatcaaagc acaagaagta
      421 ttcttcggtg atttcccatt aatgacagaa caaggaacat tcatcatcaa cggggcagag
      481 cgagtaatcg tttctcagtt agtccgttca ccaggtgtct atttccatgg aaaagtcgac
      541 aaaaacggaa aagaaggatt cggttctact gttatcccta accgtggagc ttggctagaa
      601 atggaaactg atgcaaaaga catctcttat gtacgtatcg accgtacacg taagattcct
      661 ttaaccgtat tagttcgtgc gttaggattt ggttccgatg atacgatctt tgaaatcttc
      721 ggcgacagcg aaacattacg caatacagtc gaaaaagact tgcataaaaa cgcaagcgac
      781 tcaagaacag aagaaggttt gaaagacgtt tacgaacgtt tgcgtccagg cgaaccaaaa
      841 acagcagaca gctcacgtaa cttgctgaat gctcgtttct tcgatccaaa acgttacgac
      901 ttagcaaacg ttggtcgtta caaagtaaac aaaaaattag atttgaaaac acgtctattg
      961 aatctgacat tagctgaaac attagttgat ccagaaacag gcgagatcat cgttgaaaaa
     1021 ggaacagttc tgactcacca agtcatggaa acactagctc ctttcattga taatggccta
     1081 aattcagtga cttactatcc atcagaagac ggtgtggtca cagatccaat gactgtccaa
     1141 gtcatcaaag tattttcacc aagagaccct gaacgtgaag taaacgtgat cggaaacggc
     1201 tatccagaag cagctgtaaa aacggttcgt ccagccgaca tcattgcttc aatgagctat
     1261 ttcttgaact tgatggaagg tatcggcaat gtcgatgata tcgaccactt aggaaatcgt
     1321 cgtatccgtt cagtcggtga acttttacaa aaccaattcc gtatcgggct agcacgtatg
     1381 gaacgtgtgg ttcgtgaaag aatgtcgatc caagatactg aaacattgac acctcaacaa
     1441 ttgatcaata tccgtccagt tgtggcaagt atcaaagaat tctttggttc ttctcagttg
     1501 tcacagttca tggatcaaac aaaccctctt ggcgaattga cacacaaacg tcgtctttca
     1561 gccttaggac ctggcggatt gactcgtgac cgcgccggtt atgaagttcg tgacgttcac
     1621 tactcccact atggccgtat gtgtccgatc gaaactcctg aaggcccaaa catcgggttg
     1681 atcaatagtt tgtccagtta tgcgaaagta aataaattcg gatttatcga aacaccttat
     1741 cgtcgtgtag accgtgaaac aggtcgtgtt actgaccaaa tcgactactt gacagctgat
     1801 atcgaagacc attacatcgt agcccaagca aacagcccat tgaatgaaga tggaacattt
     1861 gctcaagacg ttgttatggc tcgtgctcaa agtgaaaact tagaagtatc tattgataaa
     1921 gtagactaca tggacgtatc acctaaacaa gtagttgccg ttgcgacagc atgtattcct
     1981 ttcttggaaa acgatgactc caaccgtgcc ttgatgggtg ccaacatgca gcgtcaagca
     2041 gtgccgttga tcaatcctca agcaccatgg gttggtactg gtatggaata caaatcagcg
     2101 catgactcag gagctgcttt gttatgtaaa catgacggtg tcgttgaata cgttgatgct
     2161 tctgaaatcc gcgtacgccg cgacaacggt gcattagata aatacgatgt aacaaaattc
     2221 cgtcgttcaa actccggaac aagctacaac caacgtccaa tcgttcattt gggtgagaaa
     2281 gtcgaaaaag gggatactct ggcagacgga ccatctatgg aacaaggcga aatggctcta
     2341 ggccaaaacg ttttagttgg tttcatgact tgggaagggt acaactatga agatgcgatc
     2401 atcatgagcc gtcgtttggt aaaagatgac gtatacactt ctatccatat tgaagaatat
     2461 gaatcagaag cccgcgatac aaaattagga cctgaagaaa tcacacgcga aatccctaat
     2521 gtcggggaag acgcattaaa agatttagac gaaatgggta tcatccgtat tggtgcagaa
     2581 gtccaagatg gcgatttgct tgtagggaaa gtcacgccaa aaggggtaac agaattatct
     2641 gcagaagaac gtctattgca tgcgatcttt ggcgaaaaag ctcgtgaagt ccgcgataca
     2701 tcattacgtg taccacatgg cggcggcggt atcgttcacg atgtgaaaat ctttactcgt
     2761 gaagctggag atgaattgtc accaggcgta aacatgttag ttcgtgttta tatcgttcaa
     2821 aaacgtaaaa tccatgaagg ggataaaatg gccggtcgtc acggaaataa aggggttgta
     2881 tcccgtatta tgccggaaga agatatgcca ttcctacctg atggaacacc aatcgatatc
     2941 atgctgaatc ctttaggggt gccttcacgt atgaacatcg ggcaagtatt ggaattacac
     3001 ttgggtatgg ctgcacgtca gttaggtatc cacgtagcaa cgccagtatt tgatggtgca
     3061 agtgatgaag acgtatggga aacagttcgt gaagccggta tggccagcga tgcgaaaact
     3121 gttctgtacg acggacgtac aggtgaacca tttgatggac gtgtgtctgt cggtgtgatg
     3181 tacatgatca aacttgctca catggtcgat gacaaattgc atgcacgttc aatcggacct
     3241 tactcattgg ttacacaaca accattggga ggtaaagctc aatttggtgg acaacgtttt
     3301 ggtgaaatgg aagtatgggc actggaagct tacggtgcgg cttatacttt acaagaaatc
     3361 ttgacataca aatcagatga cgtagtcgga cgtgtgaaaa catacgaagc aatcgtcaag
     3421 ggtgaaccaa ttccaaaacc aggtgtaccg gaatcattcc gcgtattggt gaaagaattg
     3481 caatcactag gtctggacat gcgtgtcttg gatatcgaag aaacagagat cgaacttcgc
     3541 gacatggacg atgaagacga cgatttgatc actgttgatg ctttgacaaa atttgccgaa
     3601 cagcaaactg ccaaagaatt agaaaagaaa gctgctgaac aagtggaaga tgaaaaagac
     3661 gatgtcatcc aaaactttga aaccgcagaa gataatttag actaatccgg tctatgagct
     3721 gcaaggcggt cgaagatcag ccgtcttgcg gtttgccatt aaatgaaatg gagggaaccc
     3781 cttttgatcg atgtaaataa attcgaaagt atgcaaatag gtttggcttc tcccgaaaaa
     3841 atcagaagct ggtcttacgg tgaagtaaaa aaacctgaga cgattaacta tcgtacctta
     3901 aaacctgaac gcgaagggtt gttctgtgag cggatttttg gtcctacaaa agactgggaa
     3961 tgtgcctgcg gaaaatacaa acgtatccgt tataaaggaa tcgtttgtga tcgatgcgga
     4021 gtggaagtta ctcgttcgaa agttcgccgt gaacgcatgg gacatattga attagcagct
     4081 cctgtttcac atatctggta ctttaaaggg attccttcac gtatgggtct tgtattagac
     4141 atgagcccac gcgctttgga agaaatcatt tattttgctt catatgtagt catcgaacca
     4201 ggaaatacga atttagagaa aaaacaattg ttgacagaac gcgaatatcg cgaaaaacgt
     4261 gaacaatatg gacaagaatt ccaagctgct atgggtgcag aagcgattaa acaattactt
     4321 gataatgttg atctagatgc agaagcagcc gaattgaaag aagaattgaa atctgcacaa
     4381 ggacaaaaac gtacacgtgc aatccgccgt ttggatattt tagaagcatt ccgcgcttca
     4441 ggaaatcaac caagctggat ggttatggac gttatccctg ttattccacc agatttacgc
     4501 ccaatggttc aattagaagg tggacgtttt gcaacaagtg acttgaatga tctttaccgt
     4561 cgtgtaatca accgtaataa ccgtttgaaa cgtttgttag acttgaatgc acctggaatc
     4621 atcgttcaaa atgaaaaacg tatgctgcaa gaagcagtag atgcattgat cgataacggt
     4681 cgtcgcggac gtccagttac tggaccaggt aaccgcccat tgaaatctct ttctcatatg
     4741 ttgaaaggga aacaaggtcg tttccgtcaa aacttgctag gtaaacgtgt cgactactca
     4801 ggtcgttccg ttatcgttgt tggacctttc ttgaagatgt accaatgtgg acttccaaaa
     4861 gaaatggcca ttgaattatt caaaccattc gtgatgcgtg agcttgttca acgcgaacta
     4921 gcttcaaaca tcaaaaatgc gaaacgtaaa atcgaacgtc aagaagacga agtatgggat
     4981 gttttagaag acgtaatcaa ggaacaccca gtattgctga accgggcacc tacgcttcac
     5041 cgacttggta tccaagcatt cgaaccagtt ttagtacaag gacgcgcaat ccgtttgcat
     5101 ccacttgtat gtgaagctta taatgccgat ttcgatggag accaaatggc tgtccacgtt
     5161 ccattgaatg aagaagcaca agcagaagca cgtatgttga tgttagctgc tcaaaacatc
     5221 ttgaacccta aagatggtaa accagttgtt actccttctc aagatatggt tctaggtaac
     5281 tactacttga caatggaaga agaaggacgc gaaggcgaag gaatggtctt cagagatcaa
     5341 aacgaagccg ttatcgcatg gcgcaacgga tatgttcatt tacattcacg tatcggtgtg
     5401 aaccctaact cattaggtga aaaaccattt actgaatggc aaaaagaacg tacgatgatc
     5461 acaacagttg gtaagatcat cttcaacgag atcatgcctc ctgaattccc ttatctgaat
     5521 gaaccaacag acttcaactt gaccgtccaa acaccggaca aatactttgt tgaagctggt
     5581 actgatattc ctgcacatat caaagagcaa gaattagtat taccgttcaa gaagaaaaac
     5641 ttaggaaaca tcatcgcaga agtcttcaaa cgattcaaag taacagaaac ttctaagatg
     5701 cttgaccgta tgaaagactt agggtacaaa cattcgacac atgccggtat cacagtaggg
     5761 atcgctgaca tcagtgtact gaatgaaaaa caagaaatca ttgaaaatgc ccataaacaa
     5821 gttgaaacta ttacaaaaca attccgtcgt ggtctgatca cagatgacga acgttatgaa
     5881 cgtgtcattg ctgtttggaa tgctgcaaaa gacagcatcc aacaaaaact gatggaaggt
     5941 ttggaagcga aaaatccaat cttcatgatg tctgattctg gagctcgtgg taacatttcc
     6001 aactttacac agcttgccgg aatgcgtggt ttgatggccg ctcctaatgg tcgaatcatg
     6061 gaattgccaa tcatttcgaa cttccgtgaa ggactatccg tcttggaaat gtttatctca
     6121 actcacgggg ctcgtaaagg gatgaccgat acagccttga agactgccga ttcaggttac
     6181 ttgactcgtc gtctggttga cgtggcccaa gatgtaatca tccgtgaaga agattgcggt
     6241 actgaccgag gtcttgaaat ccaagccatc cgcgagggaa atgaaatcat cgaatcatta
     6301 gaagatcgtt tagtgggacg ttacacccgt aaagaagtac gtcatcctga aacaaatgaa
     6361 ttgatcattg caggaaacca attgatttcc gaagacatcg caaaacaaat cgttgatgca
     6421 ggtgtcgaaa cagtgacaat ccgttctgtc ttcacatgta atacaaaaca tggtgtctgc
     6481 aaacattgtt acggacgcaa ccttgcaact ggctctgacg ttgaagtcgg agaagcagtt
     6541 ggtacgatcg ccgctcaatc aatcggtgaa cctggtactc agttgacgat gcgtacattc
     6601 catacaggtg gggttgccgg agacgatatc actcaaggtc ttcctcgtgt ccaagaaatc
     6661 tttgaagcac gtaatccgaa agggcaagca gtgatcacag aagtaacagg ggatgtcatc
     6721 gatatctcag aagatcctgc aacacgtacc aaagaagtaa caatcaaagg aaaaacagat
     6781 acacgtacgt atactgttcc ttatacagca cgtatgaaag tagcagaagg cgatatgatc
     6841 caccgtggtg caccattgac agaaggatca atcgatccaa aacaattgtt gcaagttcgc
     6901 gatgtattat ctgttgaaaa ctacttgctt cgtgaagtac aacgtgttta ccgtatgcaa
     6961 ggggtagaaa tcggcgacaa gcatatcgaa gtaatggttc gtcaaatgct gcgtaaagtt
     7021 cgtgtaatgg atccaggtga tacagatatc ttaccaggta cattattaga catcgcagac
     7081 tttaaagatc aaaactacaa tacgctagtc tcaggaggcg ttcctgcaac ttctcgacct
     7141 gtcttactag gtatcacaaa agcatcatta gaaacaaata gtttcttatc tgctgcatca
     7201 ttccaagaaa caactcgtgt cttgacggat gctgcgatcc gcggtaagaa agatcctctt
     7261 cttggtttga aagaaaatgt tattataggt aagatcatcc cagctggtac tggtatgcaa
     7321 cgttatcgtg acatggaacc aaaagaagtc ggtgtagcaa gcgaaaatgt ctacagtatt
     7381 tcagatattg aagcgcaaat ggctgcagaa gacgcaatga acaatcaaac aaaatagata
     7441 ttttaaaaaa gaggttgtga caaattattg tcacaacctc ttttttggtt tgtctaaaat
     7501 aatttgtcta actagaagaa aagtgggaaa ggatcttaac aacatcggtt cgtatccaat
     7561 taaggtatcg gaacgatcat cttccaagag aagtcaatca acaaaagtaa agatagatca
     7621 ttttttgaat gcaaaataaa ttgatgggaa gatagagcgg ttatttttaa tattttgcta
     7681 gctattattg aataattcct cgaataaaac aaaaccagta gacatgtctg ttattctatt
     7741 tatgttttag agataagtat ccccaaataa catggattga aataagggta aatgtgttta
     7801 aaaaaacgaa ctataaaaac ttatacacaa cgtggttaaa aatagttttt tcttccctgt
     7861 ggaaaaaata gtttaacaca aacattatag ttatccacag ctatctaaaa cagatggaaa
     7921 ataagaaaaa ttcgctaaaa taccgggagg gggtaacgag ggatgagtga ttgaaaaaag
     7981 aacagagtaa ctgacttatc ctcagtgttt ctgttctttt ataattctgt tctacatttc
     8041 ttgttgttgg atcgaatctg tgagagtaat ctcggttgaa agaatgattc ttttttcttc
     8101 agggtattgg tggtggatta gttggaataa aagttctgtg gctttgattc ctaactgatt
     8161 tgtcggaatg tctatccgag tcaactgtgg cgaaaatagg gaggacaata agggatcact
     8221 gaatcctgcg atcgaaagat catcaggtat ctgctttcca gattgttctg ccactttgta
     8281 aatactcaat gattttaatg catcggtaga aaaaatcgca gttggtcttt ctcgtagctt
     8341 cattaaatca ttcattgctg ccatgctatc ttctaagcga aagccgccgt ctataatcca
     8401 ttcattacgg atatctaggt ggtgatcaaa taagcattga cggtaaccat tgagacgatc
     8461 attggataca taaacttctg tcggagcatg gagacagccg atatgacggt gtccattctt
     8521 gatcagtgtt tccaccaaat cgtaactatc ctggaaatta tccgtgtcca ctgagtagat
     8581 gttcgtgtag tcgtttccga cttttccgat taccacaata ggaatattgt attgatccaa
     8641 ttcaaaaaga agtgtttcat ccatcgtaga gcttaaaata ataattcctc ggatcatttt
     8701 ttcttggatc tttttcgcgc atttttctag ctcttcttct ggtgtttttg ccgtttgtag
     8761 aatcaagtcg aagttatttt gttcagaaac ggcagaaatg gattgaataa catctgaaaa
     8821 aaaaggattg ccataggtcg tatgagaagt acgatgagat aatgctagaa tcgcgtcaaa
     8881 accatttcct gacagtcctc ttgcaatttt gctcggttca taattcaatt ctttgattgc
     8941 ttgaagtaca cttgcttttg ctttatcaga gatatttggc tggttattta atactctgga
     9001 gacagttgat tttgagacat tagctttttt cgcaatgtca taaatagtag gtttcaattt
     9061 tatatgcacc tcctttttgg gagcgtttta gaataattcc atagtacaat agacattctt
     9121 ttttgtctat gattttgaaa atttaggaat agcttgcatg atcatgagag accatatttt
     9181 tttaagaagc tattaattat tgatacataa gctttcttat ttgtattttt gtgtatatca
     9241 aaattattta aaaaaatgct attgacagta agcgttttcc ataatatgat caaatcgttg
     9301 ggaacgctcc caaaaaagaa aggggaatcc gatgtataca aaacttttac aagcgattta
     9361 tcgcgactca gatttgtctg agaaaaccgc ctctatgatt gaacaaaaag tcagtgttta
     9421 tcagcagaag aaaggccagg caggcaaact gcattgggac gaagaagata ttttcttgat
     9481 tacttatggg gatcaatttt ttgaagagca gcaaccaact ttgcgtacat ttaagaaatt
     9541 ttatcaaaag tatttggcag atacatttag agttgtacat ttcttgccat ttttcccata
     9601 tacatctgat gatggtttct ccgttgtaaa ttatcaggca atcaacgacc gtttgggcac
     9661 atgggaagat atagaagaaa tgagaagcga aacacggtta atgttcgact ttgtctgcaa
     9721 tcatatgtct gccgaaagta cttggtttca ggaatattta aacttgaatg atacgtacaa
     9781 agattttttt attgatttgc cactggatac agatacaagc agcgtgacta gaccaaggac
     9841 cagcccgtta cttacaacgt tcattgacaa aaacgggaaa gaaaaacacg tatggacgac
     9901 ttttagcgaa gatcaagtag acttgaattt taaaaatcca gaagttttat tgaaaatgat
     9961 cgatattctt ttgttttact tagaaaaagg cgcttcattt attcgcttgg acgctgtggg
    10021 atttttatgg aaagaactag gaacttcttg tatacacctg ccagaaacgc ataaaatcat
    10081 ccaattattc cgtgcaatcg ctgatgaagc agctccagga acggtattga tcactgagac
    10141 aaatgtgcca cacaaagata atatcagtta ttttgggaat ggcgagaatg aagcacagat
    10201 ggtctatcag ttttcgctac cacctttgac ccttcaagcg attcgttcgg gtaatacaag
    10261 taagttgaaa gaatggttaa aaaccatcga tttcgcatcg aaaaaaacaa ctttttttaa
    10321 ttttttagcc tcccatgacg gtatcggatt gaatccggta cgaggtattc tgccggaaaa
    10381 aacgatcctt gaattagtgg aacagctgaa gaaagagggt gcccgagtaa actacaagaa
    10441 aaacccagat ggaacgacca gtccatatga gataaacgct acgtatctag atgcgttaag
    10501 ttctccaaca gatacgacgg aatggagaag caaacgcttt ttagctgctc attcattttt
    10561 ggtaacgtta ccaggcgtgc cggcaattta tattcaaagt gtgctgggaa gtcgaaatga
    10621 ttacaaaggc gtagaaaaaa caggagagaa tcgaaaaatc aatcggcaaa aatttttgtt
    10681 agcagatatt gagaaagagt tgcagacaga tccattaaga acagcagtat atggtgaatt
    10741 gacccagatg ctatctattc gaaaaaaaga aaaagccttc catccgaata gtttgattta
    10801 cttactggac tcagcagatt cttttatcag ttttattcgc gaagcgaagg aagagaaagt
    10861 cttagttatc cataattttt ccaatcaatc agcaggttat cttttaccaa aaggagatta
    10921 cctagacctg ttttctggac aaattatcca ttgcagtcaa gaacgaaagt tgatattcca
    10981 gccgtatgaa tatatgtggc tgaaaataat aaaataagag gtggaaaaaa atgaaaaaaa
    11041 ttattgttac tacctgtctg ctagctactg gatttcttct tactgcttgt ggtgaaagtc
    11101 aaagctctaa cgaatcacaa cagacaacga ttcaattcat gcactcttct gtcgaacagg
    11161 aacgattaag tgtgatcaat gaactggttg ctgattttga aaaagaaaat ccagacatca
    11221 aaatcaagca agtgccggta gaagaatcag cgttcaatac aaaaatcgtt acgttagctc
    11281 gttccggtaa attaccagaa gtaatggaag tcagtcagga ttttgctaaa gtgatggata
    11341 aagatgaact gattgatcag acggcagtcc aaaacgttat cgaggaagct ggagaagata
    11401 attattatga cggagccaaa aaccttgttc gttcagaaga tggaaaaagc tatattgctg
    11461 caccaattag cggctgggtt caaggtattt ggtatgacaa agaaaaatta tcagaagctg
    11521 gtttttcaga accagaaaat tgggatgata ttttaaaaat tgctcaacat ttcacagata
    11581 aagagaataa acaatatgga attgctatgc ctactgctga aggaacaatg agtgagcaag
    11641 cgttctctca atttgctttg tcaaatggag caaatgtttt ggatgatgaa ggcgaggtaa
    11701 cgatcgatac ggaaaagatg agagaagcat tgtcgtttta tcaaaactta tcccaataca
    11761 cgatgccagg ctctaatgat gtgacagaaa tcaaagatgc tttcatgaat ggaacagcac
    11821 caatggcaat ctattcaact tacattttgc cttctgttta tgaagcgggg aactcagaaa
    11881 atatcggttt tgctatccca acacaaaaag atcaagctgt ctatggcaca gtatctggat
    11941 tgacgatttc ttctggtcta gacgagaaac aaaaagaggc tgctgaagca ttcacagctt
    12001 tcttatctca accgaaaaac atggaaaagt gggtcttgat gtctcctggt ggtgctcaac
    12061 cagtgaataa acaagtagtc gaactggatg gttacaaaga aaatgaagtc atcaagtctt
    12121 ttggcgagct gccatctgag attgctagtg cttttgatga agtacaagtc tttggactag
    12181 tcggggaaaa gaatttcacg aaaatgggag atatcacaag ctcaggtgtg atcggaaaag
    12241 cagtgaacca agtaactgtc ggaaacgaag atgtcgatca agctttagct gacgcacaaa
    12301 aaaatacgaa ataacaagaa gttctgccaa tggtgttcga aaaaagtaga tagctattgg
    12361 cagattatca gggggaggtt tcaatgaatc gtccaaggaa acattctgac ttaaaattag
    12421 ccatgggact tttagcacca agcatgcttt tattagctgt tttagtgctg attccaatgc
    12481 tttccaatat tcagatcagt tttttcgata tgccgctgaa tcctaactca gacgccgttt
    12541 ttatcggatt aagcaattat cttgaaattt taagtgatag tgaattttat cgttctttag
    12601 gtttaacagt tgcttacaca ttcttagttg tagcaggcag tacagcttta ggattagccg
    12661 tagctatttt gcttaaccgc aagttcagat ttaggaaaac ggtgcgttca ctgattttat
    12721 taagttatgt tacgccatct atttctttga tttttgcttg gaaatatatg ttcaataata
    12781 gttatggaat cgtcaatgat ctgttagtag ataggttgca gcttttcaac gaagcaccgc
    12841 tgtggttcga ccagccagtt gctagcttct ttatggtcgt tttgtttgct atctggagat
    12901 actatccgta tgcgtttatt tcatttttgg ctattttgca aacagtcgac aaaacgcttt
    12961 atgaagcagc tgaaatggac ggtgcaaatg cttggcagaa gttcaagatc gtgacactgc
    13021 cggcaatcaa accagtacta gcaactgtga ttgttttacg atctatctgg atgttctata
    13081 tgtttacaga tgtctatcta ctgacgaata aagtcaatat cttaggcgtt tatttgtatc
    13141 agacagcctt tgcgttcaat gatcttggaa aagcagcagc tatatcgatt ctattgttca
    13201 tcattattgc agcggtcgtc ttgctctcac gaaagaaggt ggacttgaaa tgaaaaataa
    13261 aaagagcagg aaaatcttat tttacgcgac gattttgttg ctattagctg tcaatttgtt
    13321 tccatttttg atcatgctga tgacatcatt caaggattca caagaagcaa ttgcaacgac
    13381 acaaactatt tttccgaaag aatggacttt tgaacattat caggatattt tcaatgctga
    13441 gatttttcca ttcatcatgt atttcaaaaa tagtttagtg atctccttga tagcctccgt
    13501 aactgcagta gtgattggga tcagcggggc atacgctttg tcaaaactac gcttcaaagc
    13561 tcgctcgaaa atcaatgcta gcttttacac cgtttatatg ttttccggta ttttgatcgt
    13621 tgttccatta tttaaaatca tttccggtct aggtttgtat gataccaata cagcactgat
    13681 tatcacgatg gtcgttcaaa cacttcctac tgcgatattc atgctaaaaa gttattttga
    13741 cacgattccg actgatattg aagaagcagc aatgatcgat ggtcttagcc ggatccagat
    13801 cattattcgg atcattatac cattggctat ttctggaatc atttctgtct tcgtttattg
    13861 ctttatggtt tcgtggaatg attatttatt tgcttcgatt ttcctgtcga cctccgaaaa
    13921 atttacttta ccaatcggtt tgaacacatt attcagtaca ccagactaca tttggggacg
    13981 aatgatggcc gcttcattgg tgaccgcttt accagtcatc attatgtatg cgttatctga
    14041 acgatttatt aaaggtgatt tagtcgaagg cggcgtaaaa ggataaaaag aaaagaaaga
    14101 ggaaagaaaa gatgaaaaaa ttagttgcat tagaaccaag aatagccgga ttcaaagatt
    14161 acgaagaccg tgagatcgaa aagaacgaag tacgtatccg agtaattttc gctactccaa
    14221 aaccaggaac agaaattgtc gactttagag gattaagtcc atttatcgat gaagaatttt
    14281 cagaagaatg gcgcctattc ttgccaagag aagaaggaag ccaacgcggt gtggtatttg
    14341 gcgaatttca attaggaaat atgattgtcg gtacgatcga agaaacgggc agtttggtga
    14401 cagagtatca aaaaggagag ttagtctgca cttatggacc cattcgtgaa acgctgatta
    14461 caaacggtgt tgataattat aaactacgta aacttgatcg gttggatcaa tggaaaaatg
    14521 cagtatgtta cgatcctgct caattcgctt taggtggtat ccgggaagca catgttcgag
    14581 caggtgatgc tgtagcaatc ttcggtttag gcgctatcgg gcaaattgcg attcaattag
    14641 ctaaaaaagc aggtgctact accatcgttg ggatcgatcc catagcgcag cgtagagaat
    14701 tagcagaaaa acacggtgcc gattacacgc tagatcctat gaaagcagat gtcggttttc
    14761 gtctaaagga attgacagat aaacgtggag tagattcagt tattgaaacg agtggcagtg
    14821 ctgccgcatt acaagcttct ttaataggga tcggctatgg cgggatcatt tcttatgttg
    14881 cctttgctaa accttttcca gctgggttta atcttggtcg cgaagcacat ttcaataatg
    14941 caaaaatcgt ctttgctcga gcatctagtg aaccaaaccc agattaccca agatggaacc
    15001 gtaaacgaat cgaggacaca tgttgggaat tattgatgaa cggttacttg gattgtgaag
    15061 aaatcattga tccagttgtg ccatttgcag aaagcgcaga agcttacatg cgttatgtag
    15121 accagcaccc agaagaaagt atcaaaatgg gcgttcaagt ccgaggagga gaacaatgaa
    15181 attcggtacg caagatcaag catttttccc aagagacatt ttagaaaaat ttagatttat
    15241 caaagactgt ggatttgaag aatacgaaat cgacggtaag ttgttaatag aaaatatcga
    15301 cacagtcaaa caggcaatca aagaaacagg gatcccagta gtgacagctt gtaacggcta
    15361 cgatgggtgg atcggtgact ttatcgaaga acggcgtctt aatggtgtac gtcaaattgc
    15421 tgagattttg cgtgcactaa gcgaggttgg aggcaaagga atcgttatcc cagcagcttg
    15481 gggaatgttc acttatcgat taccgccgat ggtttcaccc cgaagcaaag aaggagacta
    15541 taaagcagtc agcgaatcac ttcgttattt agataaagta gccgaagaaa caggtacggt
    15601 tatttatttg gagcctttga accgatacca agaccatatg atcaatttgt tagcggatgc
    15661 gcgtcattac attgactcta atcaattgaa acatgtgcag atcatcgctg atttttatca
    15721 tatgaatatt gaagaagatg atatttcaaa agcattgact acgcaaaaag agtatgtggg
    15781 tcatatccat ttagcagata atcatcgcta ccaaccagga tctggcgcaa ttgattttac
    15841 tcgccatttt caaacgctac ttcaaaatga gtatgaaggt tatatggtat atgaatgtcg
    15901 tttgcgagga gaaaatgaag tgaaaattta tcaggaatca ttgcaatact tgaaaaactg
    15961 tctggaaaca gcgcgtcatt taaatgattt agagaaagaa tagagaaaga ataacaataa
    16021 gtaaataagc aaaggagaga gaaataaatg aagaaagtag caatcatagg cgctggacaa
    16081 gtagcagaaa aagtacatgc tgcttattat caaaaaagat cagatgtctt agaattggcc
    16141 gctgtggttg atccagtcag ccagcgagca gaagactttt gccgacgtac aggattttct
    16201 catgcgtata catcagttac acaaatgctg aatgagattt ctcccgatat cgtgagtgtc
    16261 tgtacaccca atcgctttca cttcgaacat gtattggctg ccctcgaagc taatgcttca
    16321 gtgatgtgcg agaaaccacc agctatgaca gctgatcaag caagaaagat ggcagagaca
    16381 gccgataaaa acgggcagat tttggcttat ggattccagc atcgtttttc tgacgaagca
    16441 acaatgatca agaatcatct tgatgttctc ggggatattt attacgtaga agcgaatgcg
    16501 cttcgtcggt ctggtgttcc aggctgggga acgtttatcg acaaagaact tcaaggaggc
    16561 gggcctttga tcgattatgg cattcacatg cttgatacag cgatgtatat tactggattt
    16621 cctaaagtaa aaagcgttcg ggcgtacagc ttccaaaaaa ttggtacgaa aaaaaattca
    16681 gggaaattcg ggaaatggga tccaagcaaa ttcacagtag aagatgcaat ttttggcaca
    16741 ctggaattag aaaacggcaa gatcatccaa ctgaatactt cgtttgcttt gaatatcaga
    16801 gaagacaaac tgaatgtggc tttttgtggc gatcaggcag gtgctaatct gtatccaggc
    16861 gaactattcg aagatgtaga tggaaagctg gtttccttac aaaagaccac gccagttaat
    16921 gacccacatg aactaagtat ggctcatttt gtgaatcggg cgctcggtga ccagactcag
    16981 atgattgcag atggctggca aggatatgtc atccagaatc tggtggaaaa tttatatcgt
    17041 tcggcagaaa caggagaagt tattacctta tgaaagtaga agaaaaacag tttgatttag
    17101 agtatatcaa taagtatgcg agtcagatga ccattggaaa tggatttttt ggtatcagag
    17161 gaagccagga ggaagactat cggaatcaag taaggggaat gtttgcagct ggtgtatata
    17221 accgaccttt aggagcagaa tcagccgagt tagtgaatct cccagatgtc atcagatggg
    17281 aaataaaaat cgacggtgaa catttttctc ttgagacgat gaaagttctt gcatacgagc
    17341 gggcactaga tttagaaatg ggagagctta aacgctatgc gctcattgaa actgctcaag
    17401 gaaaaaaagt ggaagtcatt acttgtcgtg ttgctgatca aaataatctc catcaggcag
    17461 ccctgaaagt ggtcatcaag ccgttgaccg aagactgcca agtagatgtg aaaacgggta
    17521 tcgatggaca gcaaaccaat tttggtacac agcagttggt tgaaaaagaa ctgcgagtat
    17581 ttggtgacga tttgatcagt gcatcctacc agacaacaga aagtaaaatt gatattggga
    17641 ttgcagctaa attcaataaa gcaggtgtat tccaagcaaa aaatcgccgg atcaccgctt
    17701 ccttttcaga aaagatcaga aaaaatacgt cctttacgct ggagaagatc atcgtgctct
    17761 ctacctctct tcagcaaaca gacccgttta agaaaagtat tgcccaagcc cagaaaagcg
    17821 ttagttatac agaaatttta gagcagtcga tgtcttattg gacagatttt tggcatcagc
    17881 atcggataac gatccaaggc gattcttctt tcgatcagtt agcgatggac ttcgctgctt
    17941 accatttaca gattatgact ccccgcaatg atccaagtgt tagtgtaggg gcgaagggac
    18001 taactggcga aggttataaa gggcatgttt tctgggatac ggaaattttt atcctgcctt
    18061 tctttttata tcagtttcct gaagtagcaa agaatttgct gctgtaccgt tatgatcgat
    18121 tagaaggcgc acggcaaaaa gcaagagatt atcactatca aggtgcactt ttcccatggg
    18181 aaagcgcaca cactggtcag gaagagacac ccaaatttgc tgcattgaat atcaaaaccg
    18241 gaacaagaca agaagttgct tcagctgccg cagaacatca tatcagtgca gaccttgctt
    18301 atgccgttca agagtatgtc caagcaacag aggatcaatc gtttttagaa acaaagggaa
    18361 aaacgctcat tcatgaaaca gcagcttttt ggctgagcag agctgtaaaa gtcgatgatc
    18421 atttagagat acacagagtc attgctccag atgaatacac agaatatatc gacaataatg
    18481 catatacgaa ctatctggcg cattataatg tggccttagc tgttaaatat caagctggcg
    18541 atccgctttt tttgaaagaa tgtcaaaatt ttttagataa gttatatttg ccagtgccta
    18601 acaaagaaca gctgattccg caagatgaca cgtatctcag taagccagtt attgatttga
    18661 ccacttacaa agaacgtcct ggcagccagt ctattttact tgattatgcc cgtagcgaag
    18721 taaatgaact ccaagtcagc aagcaagcag atcttgtcat gctgttatac ttattacctg
    18781 agtatttttc tcaaccaatc gtttctaaga atttgcatta ctatgaaaac aaaacaatcc
    18841 acgactcatc gttgagtaaa gcgattcacg ccatagaggc ttgccgtatc caagaaatgg
    18901 aatgggcgta tcatttattc caagaagcgt gcctgattga tttaggacca gatccgcatt
    18961 catcagatga cggaattcat gctgcttcat taggagcact atggctagca gttatatttg
    19021 gtttttcagg tattacagta acgaatggaa ctctttctat ttctccaaga cttccttcag
    19081 catgggaaaa aatttctttt ccttttattt ggaaaaatca caggttgcta tttgttttga
    19141 caaaaaaaca aatagtaata aaaaaagaga cgaaagaaac attacctatt tttgtcaatg
    19201 gagaccaata ccagctggaa aaagaactga tcttggaacg gaggggaaaa taatgcgtat
    19261 tctgattgcg ccagattctt tcaaagaaag tctgacagcc ttagaggcag cacaagccat
    19321 tcaatcaggt tttcaagaag tttttgttca ggctgaatat gatctgctac ctataggcga
    19381 tggaggagaa ggaacggttt catcattagc agacagttta aggttgaagc ggcaaacagc
    19441 agtcgttact aatgcatttg gcaaaaacaa agaaatccag tacgcttcag acggtcaaac
    19501 tgcgattttt gaaatggcag aaatcgtcgg attgaaagat gtcccactaa accaacaaca
    19561 gccacttagg ttatccacgc gaggagtagg agaactgata cgacaattgt tgcaacaagg
    19621 tgttcgacac atcatcatcg gcgttggcgg aagtagtacc aacgatggcg gcatcggcat
    19681 ggcagaaggt ctaggttatg ctttttacga tcaagataat caaaaagtag aagcactggg
    19741 agaaaatcta ggtaaaattg ctacagttga taaaacgaac gtgcctattg agctgattaa
    19801 agccaaaatc actgtcatta ctgatgttac taatctttta tgcggaaaag aaggcgcttc
    19861 ttttgttttc ggaccacaaa aaggcttgaa agaaaatgaa ttagcagtgg ctgaccaagc
    19921 catgcatgat ttttatatga gatttgcccc tcaagtaatc gaggcatcgg gcagtagtgc
    19981 tggtggtgga atgggtgcag gactgttagc atttgcttca gggaaaatag tctctggcat
    20041 cgactacgta ttggatttac tggagttcga taaacgggta cagcaagcag acctcgtaat
    20101 cgtaggcgaa ggacgtctag atggacaaag tctaaaagga aaagcgccag ttgggattgc
    20161 tagaagagta ccagagggcg ttcctgtgat agctatttgc ggaagtgttg gtaaaggaag
    20221 cgaaaaagca gcagatgttg gcatttcggc cgtttttccg atccttcctg cagctgtgcc
    20281 tttacaagaa gctttacagc aagccactga taatcttcat cgcacagcta aaaacatcgc
    20341 atctttactt caagtcgttc attttcaagg aggtaatcga tgaaaatcaa agcagcacta
    20401 ttcgatttag atggcgttct tgtagacaca gctcgttatc attacgaagc gtggctggtt
    20461 ttagctaacc agctgtccat tccattcact gagaaagaaa acgagcaact gaaaggaata
    20521 tccagaaccg agtcactaga gcgattgctg tcatttggaa aaatggaaca gaaattctca
    20581 gaaaaagaga aatcagcctt tgctgagcaa aaaaacaatc tatatcttca agcgattcaa
    20641 aaaatggatg aaacatccgt tttgcctggt gcaatagctg ttcttgagta cttgaaaaaa
    20701 acaaacatca agatcggatt gggatcagca agcaaaaatg cacgcctcat tttagaaaaa
    20761 acgaatttga caagttattt tgatgtcctt attgacggca cgcaagtatc aaaagcaaaa
    20821 cctgatccag aagtttttct caaaggagca cagcaattga atgtaccgcc aaatgcatgc
    20881 ttggtcattg aagattcaga agctggttgt caagcagctt tggctggtaa catgcatgta
    20941 ttgggaatag gtgaaaacat aaatcttcct tctgctgaat atgtgattcc tgatttaacc
    21001 gtttttgatc aagtacgttc gttttggcat ttatcagaag ccgtatcacg tatataatag
    21061 tttccgtttt ttctgtcaca agcaaaacgt tatcctcctt ttggaaatca aggtatgatg
    21121 agaaaaaagg gggattttat atgacaaaaa aagcagtttt cgcaggcgga tgcttttggt
    21181 gtatggtcaa accatttgaa gagcagccag ggatcatttc tgtgacatcc ggttatactg
    21241 gcgggcatgt accaaatcca acttatgaac aagtaacaac aggcacaaca gggcatacag
    21301 aagcagtaga gatcgaatat gatccagaag ttatttctta tgatcagttg gtggaaatct
    21361 attggcagca aaccgatccg actgacgcat taggacagtt cgctgatcgc ggtgattctt
    21421 atcgtccagt gatctactat aatgatgaag aagaacgaaa tattgcagag cgttcaaaac
    21481 aagcattgca agacagcggg cgattcgata agccaatcgt gacaaaaatc gaaccaagaa
    21541 gcgaatttta tcctgcagaa gattaccatc aggattatta caagaaaaac aagctccatt
    21601 acaatcttta tcgagaaggt tctggacgtg caggtttcct tcgtcgacat tggggcgtca
    21661 agaaataaga agccgaaaaa gctacttatg gaacaccgta tatcagaggt tgtaaaacca
    21721 gcattttgag ctaatactga gggtcagctg attgaacacc atttagtgga gaggttgtga
    21781 acctgtcgcc tagctccaag tcacaaggat ttggtaagag aacactatat atcagaggtt
    21841 gtgacaagtc gtttgtcaca accttttttc aatacctaag atgaaggaca atcagcaacg
    21901 cactgctcaa aaaaggaatg aaaggcagct tttccaactt ttttttatga cagataaagt
    21961 aaacagaaaa aacggatatt ccaaacagac tagcaagaaa caagatctga agcaactctt
    22021 ctagagacaa gccaccactc caagaaagca tcaacagagt atcgccagct ccgagatagg
    22081 attgaccata gcgaaggcac acaccgccta cgataaacgt taacagcaca ctttgccatt
    22141 ggaagacgcc ggcctgcaac caaaaaatcc ataaaattgc ccaagaacag aatagcgtgt
    22201 gagaatggac catgaagtaa aataaatcca tcaacgaaaa tagcaaagcc gaaagcatcc
    22261 atatgaacgt cgaaaaattt cttgcggaag aaaaagtaaa gaaccaggca aaaagaccac
    22321 cacataaggc ttctgccaac aaatagtaga agggcaaccg acacctgcaa tagcggcagc
    22381 gaaatcgttg gacgatgatc gagagaatcg gaatcaattc ataccaagaa agcgaacgac
    22441 agcatttgac acaatgagat ctaggataaa taatagagtg acctaacgga atccgctggg
    22501 cggttaaaca taaaaatgat ccgatacagc aaccaatgat aaaataaagt aacacgccat
    22561 ctcccccttt tcaaaaccaa gatacgcaag cttaaactaa atttttgtac atacataaaa
    22621 tttgaggtga aaaaatgaac gtttcaatcg gattagatat cggaaccaca caaaccaaag
    22681 cagttgcttt cacagatgaa gcccaagaag cagcatccgc ttattttcgc taccctttga
    22741 tccaagaaac acaagggatg gcagaacaag atcaggaatt gatctttcaa ggtgtctgca
    22801 ccgttataaa agaagtaatt aaacagttgc cttcagacac aatcattcaa tttgtctcct
    22861 tctcctctgc gatgcatggc ctgatattgt tggatgaaga gaaaacaccg ttatctcgaa
    22921 tgatcacttg ggcagataac cgagcagcgg acgaagcaga aaaactgaaa tataccgtgc
    22981 ttggcaaaga attttatcgg cagactggga cgccgattca tccaatgaca ccattgaaca
    23041 aaatcaagtg gctgcaaaac agccaacctg aattattcaa aaaaactgct tattttgtcg
    23101 gtatcaaaga ctatatcttt tatagattgt ttggcgaact gatcagcgac tacggaacag
    23161 cttcaggaac tggatatttt gatatccatg aattccaatg ggcagaagaa gtgcttgact
    23221 acttgaacat tcgaatagag caattgccgc aagtctttcc ttccacctat caagtaacag
    23281 gtctaccatc aaaaacagca gcagaactag gtttgccaaa tgatatatca tttgttttgg
    23341 gaggggcaga cgggccgctt tctaatctag ggttaggtgc ttttggtgat acgatcgccg
    23401 ctttgaccgt aggaacgagc ggagcacttc gttttattac tactcagcct cagttccatc
    23461 cagaaatgga gacgttttgt tatgttctgg atcagaaaca ttgggtagtg ggaggagcga
    23521 ctagcaatgg tgctggcatc tttgattggg cagggcatac atttttacag gaagtcgttc
    23581 aatctgctaa agaaaaagga gaaaatccat atgatgcact gctttcggct atcgcttttg
    23641 tgccagcggg tgccaatggt ctgatttttc atccctattt acttggtgag cgagcaccgc
    23701 tttgggacgc agatgcgttt ggcagtttta tcggtttaca gcggcaacat acagaaaaag
    23761 aaatgatgaa ggcgattcta gaagggatct gtctcaacct ttatcggatt ttaaaaggag
    23821 tttgccagga aaatcagctc acagagatcc gtgctacagg cgggtttgct caatctccga
    23881 tttttcgtca gatgatggct gatgtactgg gttacccgct tgttttccct actgtcacag
    23941 aagcatccgc attaggagct gtgattttag gctggagaag catcggaaag atccaaagta
    24001 tcagtgaagc acaggaattg atagaattga aagagcaagt tggagtacaa gaaactgatc
    24061 ataaaaaata tcagaaaatc tatccattat ttgtttcgac acaaaagcat ttagcacaga
    24121 cttatcagca atttgctcaa ctgcgagaag aattagctga ttctgaaaaa tgattggctt
    24181 tacggataaa aaagcgaact gaaaatcgtt ctcaggtaaa tgattttgtt gtacttttca
    24241 ttgaaagtct actattattt taagtgtaag aaaatgttag gaggcaagaa agatgaaatt
    24301 ccaacaaacc aaagaagtat taaatcaatt agttgcagat ctaagccaaa tgtctgttgt
    24361 tgttcaccaa acacattggt acatgagagg accaggtttc cttacattgc acccaatgat
    24421 ggatgagttc atggatgatt tgaacgctca gttagatgaa gtatccgaac gcttgatcac
    24481 attagacggt tcaccttatt ctacattgcg tgaatttgca gataacacaa aaatccctga
    24541 tgaaattggc cgctgggatc gtactattcc agaacgtttg gaaacacttg ttgcaggcta
    24601 tcgttatcta gcagatcttt accaaaaagg aatcgaagtt tctggagaag aaggcgacga
    24661 tccaacacaa gatatcttta tcgaattcaa aacagccaca gaaaaacgta tctggatgat
    24721 ccaagctcat ttgggcaaag cgccagagat cgacgcgtaa aaactgttgt tgaacttgac
    24781 cgaaaaagag gttgtgaaat cagcgttttg acctgagtat tagagaaaca aacgtcaaaa
    24841 tagttcctca tattttgccg ttttttgagc taataccgag ggtcagctga ttgaacaccg
    24901 tttaccaaga ggttgtaagc ctgccgttca gctccgagtc acaagaaaca attttaaaaa
    24961 acagcttctt atgttttatg gatattgtga cttgtgaccg aggagttggc aggagaacac
    25021 cgtttattca aaataagggg ttgtgacaag acttttgtca cagcctcttt ttgtttttca
    25081 ttttgacagg aaacgaccct tttatataaa agaaagaaaa gaagtaaagt ttcatataaa
    25141 tttttcaatc aagcagaaaa acgttgacat tgactagatt agcgggtaat atgttaaatg
    25201 gtattgtttg ccccacaaag agccccggaa actcgaagtg tatgtcaaac atcagaactt
    25261 aaattggagg aaaataacgt gcgtacaaca tatatggcca aaccaggcga agtagaacgt
    25321 aaatggtatg tagtagacgc aactgatgtt ccattgggac gtctttcaac agttgttgca
    25381 tctgtactac gtggtaaaaa taaaccaact ttcacacctc atttggatac tggcgatttc
    25441 gtcatcgtca tcaatgcaga caaagtgaaa ttaacaggta aaaaagcgac tgacaaaatt
    25501 tattaccgtc acagcaacta cccaggagga ttgaaatcaa tcactgctgg cgaattgcgt
    25561 gctaaaaatt ctcgtcgttt gatcgaaact tctgtaaaag gtatgcttcc taaaaacact
    25621 ttaggccgca aacaatttac aaaattgaac gtatacggtg gagcagaaca tccacatgca
    25681 gcacaacaac cagaagtttt agacatcaca aacttaatct aataggaggg aatttcattg
    25741 gcacaagttc aatatatcgg cacaggccgt cgtaaaaacg cagttgctcg cgtacgtcta
    25801 gtaccaggaa ctggcaaaat tactgtaaat aaaaaagatg ttgaagaata cattccacat
    25861 gctgacttgc gtgaagtaat caatcaacct ttcgctgtta ctgaaacaaa aggcgcatat
    25921 gacgtatttg ttaacgtaaa cggcggagga tacgctggac aatcaggagc tatccgtcat
    25981 ggtatcgctc gtgcattgct agaagttgat cctgacttcc gtttagctct taaacgtgct
    26041 ggcttactta ctcgtgacgc acgtatggtc gaacgtaaaa aaccaggtct taaaaaagct
    26101 cgtaaagctt cacagttttc aaaacgttaa tattctttgt atatcaatgt ttcaagacac
    26161 ttttcaatta tttggaaagt gtcttttttt agtttaagaa cacattcgag aacacattca
    26221 tcctagttct ttaatccttt ttgaaatgct tcgaaaacag tcttatcaac tacatctggt
    26281 gtgtttacgt atatctttgt agtagaaata tctgagtgtg ttaaagcatt agatatttga
    26341 ttcatatttg ctccgccttg tctagctata gttgcatagg tatgacgaag cttatgtgga
    26401 gataaatgag ccaattcagg atgtcgtctt ttaattgaat tgattcgata gtttaggtaa
    26461 tcagcgtgaa cagggcaatt tacttctccg gatggtttag tataggtaaa gagatactga
    26521 tctggtgttt gattaatttt taatctcaac aattgctcag cttgcactga tttccatttg
    26581 cttagtaatg ccattacaat ttcgggtact tgaattacgg tatcctttcg accttttgta
    26641 aatttctttt tttgatattt atccaaagca tgtttaaggc gaaccgtttg tttctcaaag
    26701 tcgatatatt tccattgcaa agcatacgtc tcactttttc tatcaccaag atatagggta
    26761 agcatgaaaa gagtatagtc atgaagagta agcaagtgat tattcaagtc agtgtttact
    26821 gcttctatcc aagcaataag ctcttctgac gataatgctt gattaccttc ccgtatcctt
    26881 ctttcttcta aagcaatttt tttaggtgca gtgatacatt gaataatttt ggtagttcgg
    26941 ttaaattcaa tataatttag tatttctgca atatcaaaca tagaacggac atatcctttg
    27001 ataattttta tatttgcgta ttctttcgat ttttttgtta attcatctga tattatctca
    27061 gtatttgaat ttagatagtt cattgcatac tcaccaaaca ttggaaggat gtgcaatcgg
    27121 aatatatctt tagtattact aattgttacg taacttggag ctctttttga tctagtagtc
    27181 tgacctagtt catatctagg aagccatttt gattcataga attccttaaa ttttattttt
    27241 ccttttaatt ctagtgaatt tgcattctcc tctcttaaaa ctgtttcaat tttccttttc
    27301 attgcatttt cggctttaat agccaattgc ctagttggaa atactttttg atacttttct
    27361 gatgtaattc cagtgatttc tctgaatttt tttggatacc tcagattagt tttatatttc
    27421 ccatttgaaa gttttgtagg caaaattcct cactccctta aaaagtaagg cagtatattt
    27481 cgctattctt agtatatgcg gcgagaagaa attttcaaat tgttttaaat gtattcataa
    27541 aatctttgat atcctctatg tcaaagcgaa ctataccgtc aattctgatt actggaaaat
    27601 tatatatacg aatccactta tctaaagtat tattatcaat tcctagaaac tcacaggttt
    27661 gttttttatt caagtagact ctcaatttta taaaatcatc tcctaaaaaa gattagaatt
    27721 tttgaattgc tttgggtaag cacataggaa tttcacctct ccccagttta cggcgagcct
    27781 tacggaagta tcattatttg ctgctattgt catggcattt tgttttccca gaacaaaaag
    27841 aaatctctaa gtatttgtcg ctaatcaggt catggcgtac ttgagtttct agctcgaata
    27901 gcagggatcg ttattcaaaa attgtattta attgtcaatg atcaaaaata ggttttttcc
    27961 tattcactat taataacgat aaacatgtat aaaaagacac actacgaaaa aagttaaatg
    28021 gagtttactg attttcatat ttgtatatgg aacagacata gtcttgaata atttttataa
    28081 atgttttgat gcttatcaac ttattcaacc cgctatccac tttaagtata atttttccat
    28141 acgccaataa agtagtttta ggagcatgtt ctcatttgag aacgctcctt ttttatatat
    28201 tttgaaagct acttactata cgcgtagttt caaatagttt tgtcattcta atcttcagca
    28261 acagaaaaaa atctaaatta taaaatttag ggttgactaa aatataattt ttgtatacca
    28321 tatgatcgtg ataatcatta tcatttagaa ggagaaagtt atgttagaag tgaaaaagct
    28381 taatgtttgt tataacgatt ttttagcatt gaacgagatt tctttaaaaa tacaagaagg
    28441 tgctttaaca ggtattattg gtccaaatgg tgctggaaaa tctacattgc ttaaagcaat
    28501 gttaaatatt atccctcatc agggagaggt ctccattaat aatgaagata tcaataaaaa
    28561 actggtgaag attgcatatg tagagcaaaa agctgatatt gattttactt ttccaataaa
    28621 agtaaaagaa tgtgtatcta tgggaacata tgccggaatg aaagtttttc aaagaattaa
    28681 aaatgcagaa tggcaaagag tttcaaaggc tttggaaaag gtcgatatgg gaaaatattc
    28741 taatcatcaa ataggagaat tatctggagg acaatttcaa cgtgtacttt tagcaagatg
    28801 tcttgctcaa aatgcggatt tcatattttt agatgaacca tttgtaggga tagacttagt
    28861 tagtgaacga ataattatgg atacgttaaa agaattgaaa catcagggaa aaacagtgct
    28921 aattgtgcat catgatttaa gcaaggtaaa gaagtatttt
//
LOCUS       DACEXQ010000016        27432 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.016, whole genome shotgun sequence.
ACCESSION   DACEXQ010000016 DACEXQ010000000
VERSION     DACEXQ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.016"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(287..649)
                     /locus_tag="IXH04_000513"
     CDS             complement(287..649)
                     /locus_tag="IXH04_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851147.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(723..1103)
                     /locus_tag="IXH04_000514"
     CDS             complement(723..1103)
                     /locus_tag="IXH04_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAQ1851148.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1185..2360
                     /locus_tag="IXH04_000515"
     CDS             1185..2360
                     /locus_tag="IXH04_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ1851149.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2437..3021)
                     /locus_tag="IXH04_000516"
     CDS             complement(2437..3021)
                     /locus_tag="IXH04_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1851150.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANLQRGPQDRPVHDVVIETIEISE"
     gene            3222..4220
                     /locus_tag="IXH04_000517"
     CDS             3222..4220
                     /locus_tag="IXH04_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1851151.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4332..4841
                     /locus_tag="IXH04_000518"
     CDS             4332..4841
                     /locus_tag="IXH04_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAQ1851152.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4871..5530)
                     /locus_tag="IXH04_000519"
     CDS             complement(4871..5530)
                     /locus_tag="IXH04_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAQ1851153.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5532..6296)
                     /locus_tag="IXH04_000520"
     CDS             complement(5532..6296)
                     /locus_tag="IXH04_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAQ1851154.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6304..6981)
                     /locus_tag="IXH04_000521"
     CDS             complement(6304..6981)
                     /locus_tag="IXH04_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAQ1851155.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7018..8403)
                     /locus_tag="IXH04_000522"
     CDS             complement(7018..8403)
                     /locus_tag="IXH04_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAQ1851156.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTDIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8613..8697)
                     /gene="ffs"
                     /locus_tag="IXH04_000523"
     ncRNA           complement(8613..8697)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IXH04_000523"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8720..9235)
                     /gene="tadA"
                     /locus_tag="IXH04_000524"
     CDS             complement(8720..9235)
                     /gene="tadA"
                     /locus_tag="IXH04_000524"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAQ1851157.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9342..10199
                     /locus_tag="IXH04_000525"
     CDS             9342..10199
                     /locus_tag="IXH04_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAQ1851158.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10280..10615)
                     /locus_tag="IXH04_000526"
     CDS             complement(10280..10615)
                     /locus_tag="IXH04_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851159.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10682..12052)
                     /locus_tag="IXH04_000527"
     CDS             complement(10682..12052)
                     /locus_tag="IXH04_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAQ1851160.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12130..12666)
                     /locus_tag="IXH04_000528"
     CDS             complement(12130..12666)
                     /locus_tag="IXH04_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1851161.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            12975..14456
                     /locus_tag="IXH04_000529"
     CDS             12975..14456
                     /locus_tag="IXH04_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ1851162.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            complement(14559..15266)
                     /locus_tag="IXH04_000530"
     CDS             complement(14559..15266)
                     /locus_tag="IXH04_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAQ1851163.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(15345..15683)
                     /locus_tag="IXH04_000531"
     CDS             complement(15345..15683)
                     /locus_tag="IXH04_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAQ1851164.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(15794..16756)
                     /locus_tag="IXH04_000532"
     CDS             complement(15794..16756)
                     /locus_tag="IXH04_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAQ1851165.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(16771..18102)
                     /locus_tag="IXH04_000533"
     CDS             complement(16771..18102)
                     /locus_tag="IXH04_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ1851166.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(18110..18790)
                     /locus_tag="IXH04_000534"
     CDS             complement(18110..18790)
                     /locus_tag="IXH04_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAQ1851167.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(19009..19488)
                     /locus_tag="IXH04_000535"
     CDS             complement(19009..19488)
                     /locus_tag="IXH04_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAQ1851168.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIN"
     gene            complement(19615..21294)
                     /locus_tag="IXH04_000536"
     CDS             complement(19615..21294)
                     /locus_tag="IXH04_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ1851169.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            21842..23344
                     /locus_tag="IXH04_000537"
     CDS             21842..23344
                     /locus_tag="IXH04_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1851170.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            23357..24019
                     /locus_tag="IXH04_000538"
     CDS             23357..24019
                     /locus_tag="IXH04_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1851171.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(24120..24323)
                     /locus_tag="IXH04_000539"
     CDS             complement(24120..24323)
                     /locus_tag="IXH04_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ1851172.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(24434..25618)
                     /gene="iadA"
                     /locus_tag="IXH04_000540"
     CDS             complement(24434..25618)
                     /gene="iadA"
                     /locus_tag="IXH04_000540"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAQ1851173.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            complement(25775..>27432)
                     /gene="yfcC"
                     /locus_tag="IXH04_000541"
     CDS             complement(25775..>27432)
                     /gene="yfcC"
                     /locus_tag="IXH04_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAQ1851174.1"
                     /translation="VVIFAWILTFVVPAGKFSTQDIEYKDANGETSTRTVLRQDSFRY
                     AYELDKSYVFDQLEELQDHPAEREKLDVPEKGLEKVIADGEKNLTQEKLDEISLTDDV
                     LYDQYGENIYDTSKKLHKTAKIWGTDDFGGFGFLNFVFEGLVSGDKYGSAVGIAALIL
                     VVGGAFGIIMRTGAIDAGIYAFISKTKGLERLALPLLFFAFSFGGATFGMAEEVIPFS
                     MVMVPFVIALGYDSIVAVTVTYVASQVGNATSWMSPFSVAVAQGIAGIPVLSGATFRL
                     IMWVVVTALAAGYMMIYAEKIRKKPESSLTYKSDDYFRSHIKKTSDENKKFMLGHKLI
                     LLEMLVVLVWIVWGVTQKGYYIPEIASQFFVMGLVAGIIAVLFKLDGMKVNDIASSFQ
                     SGAADLAGTAIVVGMAKGILLVLGGSDASVPSALNTILHGIGTALTGVPAVIGAWAMY
                     IFQSLFNLVVTSNSGQAALTMPIMAPLADLVGVSRQVAVLAYQLGSGFMDAFTPVSAS
                     LIGVLGVARIEWAKWARFQIKMQGFLFVLGTIFIMIAIAIGLQ"
BASE COUNT         8162 a         5741 c         4646 g         8883 t
ORIGIN      
        1 ttttttaaat tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt
       61 tatcatgtat ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt
      121 cgatcaatta ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca
      181 ctaaaaaact atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa
      241 gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca
      301 ctccctctac ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat
      361 atccttcgcc taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa
      421 cattttcctc tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga
      481 tatgaactat ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa
      541 gttccgctaa cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac
      601 catcctccaa atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct
      661 cgatcaactt gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact
      721 ttttatttct tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt
      781 aactgctccg caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt
      841 ctgcgatatg gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt
      901 ttggaatatt catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat
      961 gaatggatat ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc
     1021 tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt
     1081 atttgtccaa ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa
     1141 atagctcaaa aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata
     1201 aacagatttc aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc
     1261 aaatggatga cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg
     1321 tagctaaagc gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg
     1381 ataaactata tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga
     1441 aagaaaatat cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt
     1501 ttacaaaacc aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta
     1561 ttatagaaac aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt
     1621 tcatcatgga cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga
     1681 ttttatgcaa tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt
     1741 taagcgaatt gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt
     1801 taactttatt cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc
     1861 tactagttgc ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa
     1921 tggtatttat caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc
     1981 aacagcacga aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg
     2041 gagaatggtt aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt
     2101 tttttgaaca gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt
     2161 ggttagattt taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga
     2221 aaggaaaagt cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc
     2281 gcttgaatat tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg
     2341 ctttagagac aatcgattaa atcatttatt caaaataaaa ggacgagacc ttactaggtt
     2401 tcgtcctttt tcataattac tccgtaccat accaaactat tctgaaattt caattgtttc
     2461 aatcactaca tcatgtacag gtcggtcttg tggaccacgt tgtaaatttg cgatttcatc
     2521 tactgtgtcc atcccttcta gtacatgtcc aaagactgta tgtcggaaat ctagccaagg
     2581 agttccgcca cctttatacg cttcaatgat ttctgatggg aatccagcac cttctagttg
     2641 tccaagcata ttagctggca catttgtgtt atttacaata aagaattggc ttccgtttgt
     2701 gtttggacct gaatttgcca tagataaagc tccgcgaagg ttgaacagtt cacgactgaa
     2761 ttcatcttca aaactttcgc cataaatact ttcgccaccc attcctgttc cagttggatc
     2821 gccgccttgg atcatgaaat caggaataac gcggtgaaaa atcacgccat cataataacc
     2881 ttttttggaa agttcaatga aattttgaac cgtcttcggt gctagttcag ggaataattg
     2941 aacagtaatg tcaccacggt ttgttttgat aacagctttt ggtccttttt cactttttaa
     3001 atctagttgt gggaatgcca tattgtttcc tcctaatatt tgcatacttc ataataataa
     3061 taacataata cctaacctct cgtaaaaata ctagatttgt tttcttttat ttttcatcac
     3121 tagatacatt ctataactta ccgtttcgat ttcgaattca aacatttttg agaagcattc
     3181 tcaattaaat tctgctatag tgtgaaaagg gaggaatttt tatggaaatc tatgacatta
     3241 caatcatcgg tgcaggtcct gtagggatgt ttgccgcttt ttacgcagga atgcgccaag
     3301 ctaaaacaaa aatcattgac agtctacctc aacttggtgg tcaattagca actttgtatc
     3361 ccgaaaaata tatttacgat attcctgggt atccagcaat aaaagcaagt gaattaatcg
     3421 atcaattaga aaaacaatta actactttta accatacatt ccatttaaaa gaagaagttc
     3481 tctcgcttac tcgtgaagac gaagttatcg agattactac aaataaaggc atccattatt
     3541 ccaaagcagt cattttagct ttaggaaatg gttcattcca gccaagaaaa ttgaatttag
     3601 ataatgctga atcatttgaa aaccacggat tggattattt cgtgaatgat ttgatgaagt
     3661 atgctggaaa aaaagtcgct attgctggag gtggtgattc tgctatcgat tggacgttga
     3721 tgttagagcc tattgcaagc gaagtttact tgattcatcg tcgcccggaa tttcgtgcgc
     3781 acgaacatag tgtatcacgc ttgaaatctt catctgtcaa cttgcttact ccctatctca
     3841 tcgatggatt atcaggcaat aatggggaac tgacagatat ccggttaaaa aaagtgaaaa
     3901 gtgacgagac gattgatttg atgattgatt ctctgatcgt caattatgga ttctcttcta
     3961 atttagaaca tctttcttct tggggacttg atagtaccag aaatgccatt actgtaaaat
     4021 cagatatgtc tacttctatt ccaggcgttt atgcagctgg tgacatctgt acctatgaag
     4081 gaaaagtgaa actgattgct actgggctcg gtgaagcacc tactgcagtg aacaacgctt
     4141 tacactatat taatccaaag gaacgaactc agcctggtca cagcacaagc ttatacgata
     4201 aaaatcttcg tcctagttga ttttttatat ctctttgcat ttcaagttaa acccgaaagc
     4261 agtttcttgc atttttttca tgttccgtta acatgaaata gagaaagaaa aaataaaggg
     4321 ggagcaataa tatggtcgtc aaaactgaga ctttagaaga aaaggcaaga gaactattga
     4381 aggaacgtgg cgtaactgta gaagatatcg cacaacttgt catgttttta caaaaagact
     4441 atatttctga tttgactttg gcggattgta ctgaaagtgt caacagcgtg ctaacaaagc
     4501 gtgaagtaca taacgccatt atcactggaa tccaattaga tatattagca gaaaagaaaa
     4561 aattgatgag tccgttgcaa catattatcg aagatgacga aggcctttac ggtatcgatg
     4621 agatcctagc cttgtcaatc gtgaatgttt acggtacgat cggctttacg aattatggtt
     4681 atatcgacaa ggtgaaacct ggtattttaa aagaactaaa cagccacgat ggcgttcatg
     4741 tccatacctt cttagatgat atcgtcggag cgatcgccgc tgctgctgcc agtcgattag
     4801 cacacaaaga accagaaaaa agaagtcgcc ttactcaata attcaacaaa aaaagaaaga
     4861 aaacgtctat ttagacgagt tctttctttt ttgtttgttt caagctattt tttcctaaga
     4921 aaagaaaaac agcaaaaaat aattctaata atacaaaaaa taagataaag ctatgcatga
     4981 aaaattcttc tggaagtaca ttgatgattg gatcagtaac gggatcgaat aaccagtcat
     5041 cgttgttgaa aaacgtttca tgaaaaagga taaaaaagcg atcaaatcct atcatcatga
     5101 aaaaaccgaa aacaactggc agtaacatgc cgatttgaaa cggacgaatc aaccgccaaa
     5161 gccgatcatt cttttgcaaa tatttgataa acaaaatact cgggatcaaa gtgatgaaaa
     5221 acacagcata atccactaaa aacaaaattt tcacttcata aaagtgatgg gctccgctag
     5281 cagaaactgg gaaatctggc aagctgagga tggttttgaa gggattattg agataattca
     5341 ttaggtgatc aaaatttttc agtaacgtct cttgatctaa agaagtataa tccaaaattt
     5401 gcaaatgtcc gatatcaaat acgtatagtg gtcgaaagtt gatcgtcaca ccaactgcca
     5461 gactgatcag tgtcagaaat aggctgaaca atccagcata ttccatccac tgccatctcg
     5521 aatttttcat attagaagct ccattcatcc agtgattgtt tcacataggt agctgggata
     5581 ggcaacccag gtacatcttc ttcttttgta aaacctgata aaacgagcaa agtatctata
     5641 ttattgcgta tcccagcttg gatatctgtt tcatagttat ctccaaccat gattacttct
     5701 tctttttgca gccctaatac ttcaacggct ttatccataa tgatggcttc tggttttccg
     5761 atgtagatcg gcggcgtttg ggttgctgtc tctacaaaag agatgactga ccctgctcca
     5821 ggaagaaggc cgcgttcagt aggtatattc ttatctgggt tcgttccaat gaaagtagca
     5881 cctttttgaa tagcaagtgt tgctttaact actttttcat aggtcagata attatctaag
     5941 cctaccacta catagtcagg agcctcttct tcccatgtaa atccggcagc taaaatcaag
     6001 tcaatcaatc ctgcttctcc gataacatag acttttttcc cttttccatc tgctttcata
     6061 aaatcgattg ttgccaaagt agctgtgtag actgtctctg gagctacatg aatgtcaaac
     6121 tcatctgcta gacgattagc tactgtttct ggtgacttag tcgtgttatt ggtaacaaac
     6181 aagaaaggta attttctttt ttgcaattct tccacaaatc gtttaccggc cggtatgggt
     6241 tgtgtcccac gataaatcgt tccgtctaaa tcaattaaat atcctttata gtccattcat
     6301 ccactactct tccctttttc tgatcgtaaa tgctgttttt gttttttcta cttgtttagg
     6361 ttgttcttgt ttttttactt tttcagaggt ttttgctgac tctgtttttc gatttttaat
     6421 aaccggtttt ttgcgattag atgcgactgg tccttttttc tcatcgatat acgcacgatt
     6481 atggtttttt ttcttttttc ggcgctgagt tgtcttttct tttttaccgc cgattctctc
     6541 gatgacaaaa tacgcacaac caaaattgca atattcgtaa agatagtctt ccaaggaatc
     6601 gattcgctga tctggatgcg cccgtcgatt atccgcttgg aaaaatcctt ttaggcgtag
     6661 ttgttcataa ccccaatctc ctacgatata atcatatcgg gctagtacat cactgtaacg
     6721 ttctccaagc ttttctgcat taaatccttc acgataatcg gacacaagcc gataatgtct
     6781 ttcacctatc aaaaaattac tgtcatctaa cagtgtcact agctcgcctt tgactttttc
     6841 tgctttaggc tcctcaacga tttcttccaa agcagctgtc agttcttcgg tgactgttgt
     6901 ttcctgttca gctgatgatt ttttcttagc aatttctgtt ttatgctcaa cgttctgctg
     6961 ttcattttgt tttgcagaca tgcgttcacc acctatcttt tttattatac cttgttttta
     7021 tttgattggg tagtgagctt tcaagtaatc tcctaccaca cttcggataa attcagggaa
     7081 caaaaattca ttttttccag caatctcaat cgttggaaaa aatggaacaa acatcaaatg
     7141 atctacggta gtaaaagtat aatttttatt cagatcgatt ggcttgttct tccaaaagac
     7201 ttgatggtta atagaatcat atgtgattcc attatagacc atctgcccaa aaatcttacc
     7261 gcgaaatccc attcccttca taggatagtt acgtaaaaag ttacgatttt tttctatttc
     7321 taaaactaag cggatcagat catttccacc caatgtgaca tttaacaagt gcatcggatg
     7381 cggcaacgca gtgtgaagct gatcctgatt gacccgccct tttggaatat cagttaaaaa
     7441 cagaccactg tttaatattg ctgcttccgt ctttcctcgt tctttcaccg cctcaaaggc
     7501 tacttggata aacgaatgct cattgaagat atctagactc agagaaaatg gcaaatctgc
     7561 tacttctttt tcttttaata attcatgccc acgttctaga taactattta tttcttgttc
     7621 atcttctgta aaagctgtca ttgtttcggt agggatggct ttggctgaat gcttaaggat
     7681 ttttttctct tcatcgatgg ttaaatggac ttcaccgaca tactgcccgt actttccagc
     7741 ggctgctatt tggacatcat tgattacgtg accttctctg aataaatgat gggtgtgcga
     7801 tcctaaaata acatcaattg ctggtatttc ttttgcaatt tggaaatcat cctgaatccc
     7861 gagatgactc atcaaaacta aaacatccac ttgtgaacgt aaatcttcga ctaattctgg
     7921 caaaatatca taaggattcc gtatgtccca gccattcgga ttataagtaa gcggaaatgg
     7981 agcagtaaat gcaattagtc cgattttcgt ctggtgtttc gttgtaatga ttttatatgg
     8041 tttcgtccat gccggtggct ggagcgtatt tttatcgaat aagttatcca ggatgatatc
     8101 aaactcagca tgatcgtata aatgattcaa ctcttctttg gaatttccta cgccttcatt
     8161 gtttcctatc gtcgctgcat cataaccaac ttcattcatc agcaagatat tttcctgtcc
     8221 attcgttgct tctgacaatg gatgccaacg atccacaaaa tcacctagat ctactgcaat
     8281 cgttgtcgtt ttttctttct tttcgttttc tcgtttacgt tgatccaaaa accttctgat
     8341 ccttggccaa ttttctaaat gagaatgcag atcattcgta tgtaaaatca ctattttttc
     8401 catgtaccct ctcgcctctc tttatcttta tcataccata ggcagctttt tcttccattg
     8461 aaacaagctc tatttcagag tttttcctgc cttgaagcaa attgtataag tacaatattc
     8521 gataaatcag tctggctaaa aaatcaggaa cattcatttt tacagtacta taaaaataaa
     8581 aaaaacagac ccgaaagtct gttgtcgttt cattataatc agacacatgg cggaacctgc
     8641 ttagtgctgc ttccttccag acctgacacg cttcacaagc ccaccattgt cctagccttt
     8701 cggcaaagaa tagtatacct tatttttcat tcgttgacca gtcttttttt aatttctttt
     8761 ttcgctggcg gagctttcgg aaaaagtcag tcaaaatcat cccacattcg tcttctaaaa
     8821 tgccaccttc tacatatgct tggtggttaa accgctcgtc ctccaaaaga ttcatcaagg
     8881 taccggctgt tccgcctttt ggatcatacg cgccgaaata aacttctgct acacgagaca
     8941 aaatcatcgc gccgctgcac ataggacaag gctctaaggt aacaaaaagt gcagcatctt
     9001 ccaagcgcca gcttcccact ttttcacagg cttccttgat tgcaagcatt tctgcatgtg
     9061 tagtagcatc ttgcgtggtt tctctaaggt tatatcctct tccaatcact tttccatcca
     9121 atacaaccac tgctccaatc ggaacttcat gaagcatttc tgctttcttt gcttctgcca
     9181 atgcaaggcg catccatttt tctttttcta ctgcatcaag atttgtattt accaattacc
     9241 cttccctgcc tttgtttttt cttgttttac tttagcatat gataaaaagg ctgtaactaa
     9301 cttttttttc tatggtaaaa taaatacaag aggtgaacaa gatgaaaatg gataaattac
     9361 ttgctcttta cccacaagca cagaaaaaaa actctccagc atcagaagcg gatatccttt
     9421 cttttgcaat tgatgatgct tttatctgga tcaaacaaga ttcattatct caacaagaaa
     9481 ccagcctttt gaaagcgtta ttccctgtaa tcaacgatca aaaaaaacac ccatggtatc
     9541 actatctttt taaagatgct tcttgtgttc aagaaaaaag ctttcgtttg atccagcttc
     9601 acgtcgaatc aaaaggagaa ttcttaaaaa aagaatggca agatactatt ttggaattat
     9661 ttcgctattc tgctgatttc tttttttata cagacacaga tgcgctctta gtcgaacaaa
     9721 taaaccgtga ttatcttgat gctactgaaa tcaacggtat ttttctatct ttagatgctg
     9781 attttgatac gacaacgtct gtattcgttg gttctttcca ttcacctggc accgacctcg
     9841 tccctttgtt tgctgaagaa agaaaaattt ttttaacaga gcaaaacaat ctttatactc
     9901 acagccgtac tttttctatg caagatgtgg ctttacatta ctatacaaaa gatactgtaa
     9961 aagatagtaa gttgatcaaa agttacatag aactgatcaa acaaaatgaa atggaagtga
    10021 tcattcaaga attatggaat gaattaggca atgtcagctc tgctgcaaaa acactgtttc
    10081 ttcatcgtaa tactctaaaa tatcgaatcg aaaaatttca agaacagtct ggtttcaatc
    10141 taaaaaaagc caacgattta ctgttttgct atcttttatt gcttcatgaa cataattaaa
    10201 gatagattaa aggggttgtg acaataattt tgtcacaacc ccttatttcg aatgaatagt
    10261 gttcaaaagc tagtttctct tacatcattt tttcaacaaa atgcattagc cggtctttta
    10321 catcttcggt tgaatctttg attgaatcac cataaacaga gattttcttt tttcctgatt
    10381 tgatttttgc taacattccc atcaaagaat ccaagtcact atctgaaaga ccatctacga
    10441 tttctagtaa tttttcattt ccatcaaatt tgtcatagac gaattttttt actttcgtcc
    10501 gagtagtcat atgatggatc ttttccatta cttttcccga tacaacgact gctgtcgcta
    10561 cactggcagc ggctgctacg cttaatccga ttgttacttt tgttgatgtt ttcataagta
    10621 tcagctcctt tttatctatc tccatcatag catagacaaa aaaataaacg aactaatcca
    10681 ctcagtgaaa tctcaatcct ttagggaaaa agttctttac tgttcctttt acttgtttcc
    10741 caaatccgac aggtatctta tctaatacta gcaaaaccca tccttgattc ccatcacgtt
    10801 gaaatgtttc tcctgctgtg taagattgcc attccttttg agtgatcggc aagcaaggta
    10861 tattctctat cttttttgta gcaagcgcta atgcatagct aggttcaaat cgattctttt
    10921 tgaaatctcc taaatgcaaa cctgtacgta cgacttttaa accatcgaga gaaggggcta
    10981 actcaggaac ttcccaaaga tggtcgttga acactaaaag tcttccagta gcttcataat
    11041 gaaaatcatt tgaaaactcc gtccagagtt tttcctgctc ttttgtcatt tgcactttac
    11101 ttttttttcg tgtcttcttt tctttatgca tctgattttg tccatgaaaa gtcagctttg
    11161 caacaaaatg tccttcaccc tgatctttat gaggccatat acgtattgtt ttttccaaac
    11221 cagctacgct tccccattca gaacgtcctg agctaacgga ttgagtcaaa ggaatctcct
    11281 caatcgttac tggataattt tctacaagcc agctaatgat ctcttcattt tcttctggtg
    11341 caaacgtaca agtcgaataa atcagttgtc ctttattttt tagcattttt attgctgaag
    11401 acaaaatctc ttgctgacgt ttttgacagt atagcggaga ttcttctgtc cactctttta
    11461 ttgcatttgg atcttttcgg aacattcctt ctcctgaaca aggagcatca accacgatcc
    11521 gatcaaaaaa gcccgaaaaa tgagggacaa gttctgcggg tgcatgattt gtcactattg
    11581 cgttcgaaac tccccatcgt tctatatttt ctgataaaat cttcgcacgt ttcggaaaga
    11641 tctcattcgt cactaataac cctttcccct tcatttgagc agctaattgg gttgatttac
    11701 caccgggagc cgcacacaaa tccaaaactt tttcaccggg ttttgctgca gcagctgttc
    11761 caacgatcat tgcacttggt tcttggctat attcataccc agcttgatgg aggaaagact
    11821 ttccattgac agtgccaaga aatccctcat tgctgtaggg agcgggttgt aattcttcgc
    11881 tatggtaagt ttggaccatg tccaatcctg ccggttttag tggattccac cgaaatcctt
    11941 ttttcacgct tccttgttca agcgcagaaa aaaaatcgga tgcttcttcg cctaaaagaa
    12001 gacgatattt tttgataaat tgctgtggta acgttgtcgc ttcctccttc attccaatgt
    12061 ttcgttagac attttacaat cttttttacg aaaaataaag gtggctgatc tactttttca
    12121 tgttgctgcc taaggtgact gttcctttaa gatattttcc aagtattcat ccgtcacctc
    12181 gtttttatcc caaacagcgt tcatcatttc aagaaaatct tgagaagaat catctgagcg
    12241 caaacttccc aatgcattaa gccataccaa ttcaagcgac ttttcttctt tgatcttcgt
    12301 gtgacctttg acatatgaga aaaatggccg cttaaatagc gaatccatgt ctaaatcaga
    12361 ttttgcaatg atttcacgaa tactttcatg gatagaaaaa aaatctttca ataagtagac
    12421 aatgcttaaa acatcatcgg aactgcattc agctaagtag ttttcaaaat aaacagtgat
    12481 ttcttctaat atcttctctt ttaacccgct gattgatttg aaatgtgtat aaatcgggta
    12541 tgtagaacaa ccgcagaact ctgctaactc tcttgcagta attgacttca ttccatattc
    12601 ttccacgtat accatacttt tgaacagcaa atattcttta ctataatttg ttactctccc
    12661 catcattctt acctccgtct cttttcatct acccataaat aaataacata aataattcta
    12721 aaaatattat tgataattat caaaacatat ttagttatat ccattaaaaa tcaatttttc
    12781 cgccttaaaa acaagaatat cttgacaaaa taccctagga aataaacaat agaataaaca
    12841 aggtaacaaa aaaattaaat aacgttatat aaaaaagaag gcgacactct gtgagaataa
    12901 cacagactga aatgactttc cactcacagt ttttgtctaa ctatatacaa aaacagaata
    12961 aaaaggaggt atttatgcta attgaagcaa gaaaaaaaga gttacttgtt ctacaaatgg
    13021 tcattcaaaa aaataattgt tctttacaag aattatccaa tgacttacat attccaaaac
    13081 ggacaatcaa agaactgatc cgaaaaataa atgtatcact caagcaattt ttattgattg
    13141 atgaatttat ttattctaat cataaaggag agatccaaac caaagaaacc gatcaaacta
    13201 aaaagttagc tgttttttct aagttaaaat tatgctacct aaaagaatcc aatcgtttta
    13261 actatttgat gttactaatc aattatccac aaactgctgt accaaaaaaa tatttacttg
    13321 aacaacttta tatatcgccc tcctatctcg aaaaactgac aaaacagctc aatcattcct
    13381 taaaaaaatt ccatttacgg atcctatctg taaaaaattg ctgtactctt gaaggaaatg
    13441 agttatctat ccgtctctat ctttactttt ttctttcaga tacatttgga ggtaacgatt
    13501 ggccattttc ctcacttgaa ttaaatgcac taaaaatgaa cagtacagat gagcaaatag
    13561 aaaaaatttc aaaacaagaa aaaaatatct acttgctgtt aactttattc atattacgaa
    13621 acaaacaaca ggcatatttt ccaaaaactt cttctgaaat cactgaattg atggacttat
    13681 tgcagcatac acatgattat tcagaatact ttacttcttt ttcagtgagt cgcccttgtg
    13741 aagagacaga aacagagaga gcctacttta attatttttt ctattgtttt tttccaaatc
    13801 aaatctcttt tcgacaaaaa caggagattg gttttttatt ttccacaagc aaaaacctta
    13861 tttgtcgaca agtcggtcaa ttgatgactt acttaactca acagtttcct ctcaaccatt
    13921 tagaaaataa aataccaatt tatttttatt ttcttgtgtt ggcagttgtt tttttcgaac
    13981 tgttgggtga atccagcagt agtttttcta cactttgctt tccagaagcc acgtccaatc
    14041 attctagttc ttttatacag gaaaagaaat taagagaaaa aatcgataca attttttcaa
    14101 aaaatgggga aagaaaccaa atcagtcaat accttagtca attgatccaa cacttactac
    14161 aaacagaaac gaaagagcag ttactcatcc atttgcagat aaccaaacag tttacaggaa
    14221 attatcatct tcaaagccat ttgcagcagt tatttcgcaa agatgctgtt aaaattaccg
    14281 catattcaga acatgctgat ttgatcatta cagatcagca tgagaaaatt tcagatgaaa
    14341 cggagacttt ctatctggat acaacgcaca atacagtgtt atttggcaag ttaataaaag
    14401 cggttcatga actgtatatg aaaaaaggtt atgagaacaa aacaaagagg atgtgacaaa
    14461 attcttgtca catcctctta ttccaaataa atggtattca atcagctaac tctcggtatt
    14521 agctcagatc aaagcgctga tttcacaact tctttatttt aatctcttcg gttgccgccg
    14581 aacaataaga tcaagcgcaa cagctgcagg aatgtcgaaa gcgctgctgc aacataagtc
    14641 agtgctgctg caaataatac gtgtcttgcc atcggtactt cttcttccgt cagtagtccg
    14701 ccttcactca aaatttggat tgctcgccga gacgcgttaa attcgactgg taatgtcacc
    14761 aattggaaaa gtaatgctaa agaaaaagcg aaaatcccga tattgattaa cgtagagttc
    14821 cagctaagca atacacctac cataatcaag gggaacgaaa tcatcgaacc aaagttagct
    14881 gctggaacaa gtgctgcacg aatcttcaat gggacataac ccgaagcatc ttgtaccgca
    14941 tgtccgcatt catgagctgc tacacctatc gcagcaactg aagtagactg tgcagttgct
    15001 tgagacaaac tcaacatctt attacctgaa ttgtagttat ctgtgagatc tccggcaatt
    15061 tgttgaacac cgacattatt tatctgttga ctttgtaata tatattgtgc tgcttgtgta
    15121 ccagtcacat tgtttttgct tctcacttga tcgtattttc ggaaagtact attgacgtac
    15181 gctgacgcaa ttccggaaat aactaatcca agaatgacca aaagaaatgt cggatctaaa
    15241 aaaccataca ttccataacc atacattttc gtttcactcc ttttcgttta ctttatttta
    15301 tcattagtag taggaaaaga atgtgaaaag accttgaata ttctttaatt ctttttcaca
    15361 aattccgatt tcagtttcat cggtccaaat ccttctacct tgcaatcgat attatgatcg
    15421 ccttctacta aacggatgtt cttgactttt gttccttgtt taatggcacc tgacgcacct
    15481 tttactttca agtctttaat cactgtcacg ctatcgccct ctaacagacg gtttccattt
    15541 gcatctaata ccactaatcc gtcttctatg acagtttctt gttcttcttt gttccattcg
    15601 ttcccgcatt ctgggcaaat caacaagcct cgatcttcat atgtgtactc tgagccacat
    15661 tctgggcaat ttggtaatgt catcttcttt ctcctttaaa atctttagtg agcactattg
    15721 taacggaaaa agaagatctt gaaaagatac atttgtgatt tttactacaa aaggcacgtt
    15781 ttcgaacgaa gtatcattct gtttttctgc tcttatacga ttggcatata tcttggatct
    15841 ctttatagat ttcctctact tctgctaaat cttttgcatt cgcaccactt gctaatggat
    15901 ggcctcctcc atgatggcgt ttagccaatt cattgatgat cggcccctta gaacgtaaac
    15961 gtacgcggta ataccctgaa ggctgctgaa caaaaatccc ccaagccagc acttcgtcaa
    16021 tcactccagg aagtgaaacg atcgaagctg tctctgaatc atcgattcca tatttatcaa
    16081 gaatttcttg cggaaggatt attcttcctg caccattttc atccacctgg atattttgat
    16141 aaagataacc ggaaagttta gcgactttca tcggcatttg ttcaatctct ctgttcaaag
    16201 cacttgcatc aaatggataa tttctcaata tcgaagcaat ctctaatgta cgggacgtcg
    16261 tggagggata caaaaagcgc cctgtatctc cgacgattcc tgcataaagt aaccgtgcag
    16321 ccttaacgtt catctttaaa gattcaggaa acatcaaagc aaattctgca atcatctcac
    16381 tacagctact tgcttttgta tttacccaca caagatctcc ataagggtca tcattgggat
    16441 gatggtcgat tttgatcagt ttttctgcca actgatatct ttgatcactg attctgggtg
    16501 aattagctgt atcagtcacg atgactagcg ctccttggta gaagacatct tcaacttctt
    16561 gcatttctgc taaaaagcga agcccttcaa tcgtttcacc tacttgacgt atttcttttt
    16621 ctgggaaact ttcccgtaat agttcggcta atcctacttg tgatcctaat gcatcaggat
    16681 cagggcgcaa atgcctgtgg ataatgattt tatcgtattt ttttattgta tctactattt
    16741 cttgctgtac tgtcatttca ctcctgcttt ctaagaacgt tccattactt gacaaacaac
    16801 gattgctttg gaaacgatgg tgttttcaat aaacacttcg atatccagtt tagcggaacg
    16861 tctgccgatt tccaatacac gtggacgaat gtctaattcg ctttccaatt ggatcagccg
    16921 caaataatgt aggttcactt gttcaatcaa gatatttcgt ttttggttca tcaccattgt
    16981 tttttgtgcg acatcagaaa tgatttcgct aagtacgcca aaagaaatgg ttccaacgcc
    17041 attgaccatt tgtggtgcta ctacgaattt gaacttaggg ttttccaaac gatttccttc
    17101 acggtcagta tcaatcgtat ggatatttcc agatatctga tccgaaatcg tatctgcaat
    17161 ctgcggttgt cgctggacaa gctgcatcgc tttcattaca tcttgccggg taatcaagcc
    17221 ttccaatgtc aagtcatcgg aaacgaccgg catgacttct agcccatccc agatcatttg
    17281 atggctaaca gaggcaatac tcattgtttt ctttacactg attggttctt tcgtcatcac
    17341 ccgatcgatg atctggtttg gcgatttccc taaaacatct tttgccgtaa caatccccat
    17401 caaacgatga tggcggttga cgactggaaa acgcgaatgc tgcgtttctt ctgacaaacg
    17461 ctgataatca gaaatcgtat ctgtctgctg caaataatga gtcttttcag ctggcatata
    17521 gatatcactg acaagcatga tatctttttt gatcaattgg tcgcttaacg ctcggttgat
    17581 catggttgcg actgtaaatg tatcataaga ggtgcgtaag atcggcattt caagatcatc
    17641 agctagcttt gcaatctcag cagtcgtatt gaatcctcct gtgatcaata ctgcggcgcc
    17701 atcttccaat gctaattttt ggacttccgc ccggttgccg acgatcatta gtgatcctgg
    17761 cgtaatatag cgttccatgg catgtttagt catcgcgccg atgacgaatt tattcaagac
    17821 cttgtctaat cctgccgccc cgcctaaaac gtccccttca atgatcctga cgacctcgcc
    17881 aaatgtcaaa cgttcaatat gtttttttaa tttgcgttcg atacggatgg tccctactcg
    17941 ttgaattgtt gaaactagtc cgacattttc tgcatctttg attgcacggt acgctgttcc
    18001 ttcactgaca ttcaattctt ttgcgatgct tcttacagaa attcgattgc caacaggtaa
    18061 actttcaata tattctaaaa tcaaatcatg ttttgtcgcc attttctgcc tacacttcca
    18121 gcgtttccga gacgctcatt atttttcctt cactatcttc cagtaactct acaaaagatt
    18181 cgggatcttg ttcaatgaca gggaaagtat tgtaatggat tggaactact ttcttggcac
    18241 gaattgtttg cgcagccttt gctgcatctt ctggccccat cgtatagtta tcgccaatcg
    18301 gcaagaaagc gacatcaata tcgaattctt ctgcaagcaa tcctagatca ctgaaataag
    18361 cagtatcccc tgcatgataa atcgtttttc cttctgcttg aataataatt ccgctaggct
    18421 cgcccatata ctgaacgata ccgtctactt catagcttga actatgctgt gcatgtacga
    18481 attttaccac gccgaatgga aacgcatgtt ttccaccgat attcataccg aatgaatcta
    18541 ctcctctgct gttcgcataa tcagcaatct caacaatact gataattgtg gcttgatttt
    18601 cttttgcaat aggaatcata tctcctatgt ggtcactatg accatgagta atcagcaacc
    18661 aatctgtctt tacttcttct gcagttaagt ctgtcaatgg atttcctgta ataaacgggt
    18721 caatcatcac gcgttggcca ttttccattt caattgttaa acaggaatgt ccatgatacg
    18781 taattttcat attttacacc tcgcgttttc tctgtatctt ttttattaaa aacgatttca
    18841 tcacttgcac atcaatgaaa aaatatagct gctaaatata ttttcattgg attgatagta
    18901 cagtatgtac aaagcaattg taacagataa tacaggatgc accaagtacc aagcagatat
    18961 aatctcaagt ttgaggtctc tgctctgctt agcttctctt ctcgtccctt aattaattaa
    19021 atcagcgtaa ataggatgga cgatcttttc taaatcggct acttttatcc gaaggcttct
    19081 cccgatttca ccgcttgaca caattacttc ttctttttct tttaaagaac tgtctgctaa
    19141 aaaacaataa tcaggatgtg cgagttttat tccaataggg gtgtttgctc cgtggggata
    19201 tcctgtatgc attaatacaa aatccattcc aggaaaacca actttacgat ctttcgtcac
    19261 ttgtctcgtt tttttgtaat ctaagtgatc gttcaaagga atcaatgcga tgatcacccc
    19321 agttttgttt cctttcacta cgagagtttt acatatcaaa gctggatcaa tccctctttt
    19381 ttgtagttct tctgtgaaag attcttctac cacatgacta aaatcaagtg gttgatagct
    19441 gatcttttgt tcttctaaat aacgttcgac ttttgtttga ctcatcattg attcactccc
    19501 tttattctat cgtttattat aaagcgaggg atgctgtatt caaaaattat ttgtttttcg
    19561 caaaaaaaga gcaagacgag cactctatag agaggttcat cttactcttt ttgtttattt
    19621 tttatttcga cgttgagtag cttccataat gactggcaag atcactgggc ggcgttttgt
    19681 ttgttcaaat aagtaacggc ctaattgatc tcggatgtct tgctttaatt tgctccactc
    19741 aaaatcattg ctttctaaat gtttctcaac gattgtttca atcatattgc tgctttcttt
    19801 catcagatca cggctagctt ttacgtaaac gaatccccgt gaagtaattt gaggttttga
    19861 aatgattttc ttttctcgcc gactgatagt gacaaccgct acgaaaatcc cgtcttctga
    19921 caagattttt cgatcacgta aaacaatgtt tccgatatca cctatgccta atccatcaat
    19981 cattacattt tctgcacttg tagttcctgc aacactcata cgcttgttct tatattccag
    20041 cacgtctccg cgtccagtaa tatagatatt tttgtatggc atccctactg catgagccaa
    20101 atctgcatga gcagctaatt cacgatactc tccttgaact ggcacaaaat atgttggttt
    20161 catcaaattg atcattaatt gcaagtcatt aggattggca tggccagaaa cgcgcatatt
    20221 ctcagagatc aacttcactg ttccaccagc tcgataaatg atatcttccg ttttcgccac
    20281 gatggtttcc atcgcgatgc ttggcgtagt agtgatataa accaaatctc cttcttcaat
    20341 cttgacatta cggtgtgtac cgtttgccat tttttgcaga gacttgatgg gttcacccat
    20401 tcggccagtt tctaaaatga tcagttcttc tggatcatag tttttcatgt ctttcaacgt
    20461 aacaagcaga tcttcacttg gtaattgaag tttttctaaa cgcatcgctg tgcgaataat
    20521 tcgttcgaaa tcttgaccag tcaagacaac ttttcgttgt gctttttctg ctgcatccaa
    20581 tacttgttgc actcgttgca aattacttgc tacacaagcg acgatgatac gcccttccca
    20641 atatttgatc gtatcaaata cttcatctgc tatttgggct tcagaagcta caggctgagg
    20701 attttctgca tttgccgatt cacttagtaa ggctaagact ccttctttgc cgatttccgc
    20761 caatcgtgca taatctgttt gatacattgg gatagccgtt tggtcaaatt taaaatctcc
    20821 cgtataaacg atactacctt cttttgtttt cagattgatc cccactgaat ctggtatcgt
    20881 atgagtggta cggaaaaagc tcactactgt ttcaccaaag tctatttctg tatgctcatc
    20941 taccacatgg aaatccttaa atcctttggc tgcttcatta cgactgacat tcaattttgc
    21001 taactcgatc gtcaactctg tcccgaatac tggtacagaa atttttgaca acaagtaagg
    21061 aagtgccccg atcgcatctg catgtccatg tgttaaaaag acaccagcta cacgatcaat
    21121 gttttcttct aaatacgtaa aatcagggat tacgacgtcg atccccaaca actcattttc
    21181 aggatatttt agcccacaat ctaaaataaa aatatcctcg cccacttctg cgacatacat
    21241 gttttttcca ttttcacgaa cgccgcctaa tgggataatt ttaatggtac tcacaaactt
    21301 cacctcttct tatgacatcc aagtttaaag atgtcgattt tcttctttat ctaaatatat
    21361 gagccattcc aatgaagcat atgcaaaatc actatttctc aacttatgga gaatcagcag
    21421 gaaaattacc agcacatccg cgttctgaca gttggatggg cattttattt tggatatact
    21481 tcattaacaa aatggatctt ttgcgaaacg ctatagccag aatacttata taagtattca
    21541 gctgttcccc attattatac aacaaataaa ctaaatcgct agtatcagca attgttagaa
    21601 caactatgca cgtaaaaatc cctctgtagc atctaggatt tcttttgcta ttcctctttc
    21661 atttcttctt cgtcggtttg aaacatactt ttttcatttt accacatttt ttgcaataac
    21721 tatagtgact ttttatatat acagaacctc atgttgatgg tgtgcaaaca gcataagatt
    21781 taagctagct ttaagatagg ttgtctatta tcaaatgcat ctactaggag ggatgcaaaa
    21841 tatgaatttt atgaaacgtg catggaaaag tacaaaagta aaatggggac gttcattgtt
    21901 attgtttgcc gtgttcacag ccattcttat ttttgtgcta gctggcctca cgattcgaag
    21961 cgctgcagaa gaagcagcga atcaagcaca aaaagaagtt ggagcaacag tgactttaag
    22021 tgccaatcga gaagcttcat ttaaaaaaca ggaacaaact tcttcaagcg gttctaccac
    22081 tgaagaacgt ccagatccag gcagtttttc tcttacacca gtttccttaa cggacgcgga
    22141 aaaaatagct gaattagata atgtaaagag ttattccttt gaaagttccg cctcagctat
    22201 ggcaaaagat ggaataactg ccatcactag ttctgattca agtgaagaca gttcagaaac
    22261 tactgatgca ggcggtgaac ctgctggagg aatgggcggg aatccgcaaa tgactcaagc
    22321 agactttcaa atcagtggtg tgtcagcaac tgcacaaaat agtaattttt ctgatggaac
    22381 agcaaaaatc acggatggtg aaggaataga tgacagcgac aaaggcacaa acaatgtagt
    22441 cattgattct actctagcag aaacaaatga cctggcagtc ggagatactt tcacaatcac
    22501 gagtactgaa gatgaagata ccacttatga aatgacgatc aaaggaattt atgaatccag
    22561 cgaaacaagc ggcagtatgg gtatgaaatt caacttcatg aatccgtcga atacacttta
    22621 cacttcttac acttttgcaa atgaactaaa tgggacatct gacgataaca cgatagattc
    22681 tgctatttac actctatctg atccagataa aatggatgag tttctcaaag aagctgagaa
    22741 gctgatcgac acagatacat ttagcttaca atcaaatgat tccatgtatc agtcaatgtt
    22801 ggaaccttta aacaacgtgg caagtttttc taaaaatgtt gttttactag tggctgtagc
    22861 cggtattatc atacttactc tgatcattat gattaccatt cgggagcgtc ggcatgagtt
    22921 aggcgtcttg ctatctttag gagaatccag aagtaaaatc attctccaat tgtttaccga
    22981 agtggctatt tgtatgattc ttgcattagg agttgcttcg cttagcggaa acgtcgtcgc
    23041 gaatgccgtg ggacagcagc tacttgacca acagacagaa accacgacac aaaaccagca
    23101 agctcctggc ggtggacaaa tgccaggtaa cagacaagaa caaggcggtc aacgcgatgg
    23161 tcaacaagga accacgaata atccatttgc tgtttctgat caagtcagcg aattggaaat
    23221 caatgtcaaa cctgcacaat tgggactatt agccggagta gcatttggaa tcagtctgtt
    23281 ttctgtcttt ttagcttcta tagggatatt aagattaaac ccaagaaaaa tcctattaaa
    23341 ctaaaggagg aatttgatgt tacggacaga acaattggga ttttggtatc aagatccgca
    23401 agatgcgctt tataaagatg tagaactgaa tttcgaagct ggaaaaatgt acgctatttt
    23461 aggagcaagt ggttctggaa aaacgacttt tctttcgctt atttctggac tagatgcacc
    23521 aaaagaagga aaggtatact atcaagatcg ttcactgaac aaaatcgggt taagaagtta
    23581 tcgtaaaaat gatgtctcga ttattttcca agcttacaac ttgcttcctt atttatcggc
    23641 tctagacaac gtcatagcag caatggaaat cactaagacc aagcaagaga attatcgaca
    23701 agttgcttta gatcgactgg ctcgtgtagg aattgatgaa atattagcaa aaaaacaggt
    23761 gaccaaactt tcgggaggtc agcagcagcg agtcgctatc gttcgtgcta tctgctgtga
    23821 acatcaacta atcgtagcgg atgagcctac tggaaatcta gatgaaacaa cttcacaaga
    23881 tatcgtcaaa cttttccaag aaatcgccca tgaacaaaat cgctgtatta tcatcgtgac
    23941 tcatgagatt gaagtagcaa aaagctgtga tgaagtcttt gaactaaaaa ataaaaaatt
    24001 tcaaaaaatc gaagtttaaa aaacaggata atgatcaaat aaaaaatccg aaccatatct
    24061 gtctcgcaga tatggttcgg attttttatt tgcttgattc aattagtaat aagtggtttt
    24121 tatttttttt cgtcttttcg agaaaacttg tctttgactt cgtcgcctaa atttcgagct
    24181 ttttctttga tgtcagcacc tagtgattgt gctttccctt ctgtttcttt gctcttgtcg
    24241 tctgttgctt tgccgtatgc ttcttttcct tcaccgacta ctttatcttt catatcttta
    24301 gaacgtcctt ttaaatctgc cattgtgctt cctcctctta gtttgtttat tgatctacct
    24361 tcagggtaac atcaacaaca gatacagaca aacagaacgc tcgttttcta aaaaagatag
    24421 ttagagctgt tgattaaaaa cttttttcga aagtccccca tactttgact tctttttctt
    24481 ggaccattat ttctccttta gcgataactg tatcaatatc aagcgtttct ttatccaaga
    24541 tacacagatc tgcatcagca ccaatttcga tttttccttt tttatccaat ttcaatatac
    24601 gtgctggatt actggttata gctcgtaagg cgatttccaa agggatactt tctttttgca
    24661 cagcttcttt gatccctact agcaatgcct tggcacttcc tactcctaac cctaggaaat
    24721 gattgttttc atcaaaataa ggcaagcttc cttgtccatc tgaggtcatc gtgaaattat
    24781 ctaaagaaac atcttcctcg atcaatcgtt tcagtgcttt gctgaagcga acttcaccat
    24841 ctgtcttctc ccaaaaatca ggatcttcgc tagcagtgaa gtcaatcgtg ccaccacgtt
    24901 tggcaaatgc cacgcaagct tcaaataatt ctggtgtgcg atttgcatga gtaggcaaaa
    24961 attgtgtgat cggaatttct gtttcttcta cgattcgtgt caatagctct agctttcttt
    25021 ttcctccgcc taagtggaca ttgatgatcc cagcttttcc ggataacatg cctcctacac
    25081 gagcatctgc tactgcacga gcgaactctt caaaacttgg ctgagaagaa cggtgatcgg
    25141 atactgcgat ttctccgatt ccgataattt tatcaattgt caaaaagtct ttgatgatag
    25201 aatctgttac tgtttttacc ggcaatcggt acgaaccttc gtatacataa gtggagattc
    25261 cttctgcttc cagacctttc gctttgctga tcaacgcagt catatctcta ccttctccgt
    25321 ctgttcccaa acaaccgaca actgtcgtta caccagctgt agtcaattgg ctcaatgtga
    25381 cttctggcgt tcgattttga taaccgcctt ctcctcctcc acctaaaata tggaaatgac
    25441 agtcaatgaa acccggggtt agtacttttc cctcacctga aagttcttct acttccactc
    25501 cttcgtagcc gccttctagc tgttcttcta cagccaaaat cttcccaccg gcaatcagta
    25561 catccatttt tcctaagtaa tctggtgcaa acacttcaat ttgtcgaatg attttcatac
    25621 atttttcctc tttctttaag tataactgat gataggatct taaaaaataa tccaaacgat
    25681 aggatcttta ctcctttgat tttaccgtaa aatgacggtt cttggagtcc ttatctactg
    25741 tttggattac ttttttattt acaggtctcc agtactactg cagacctatt gcaatggcaa
    25801 tcatgatgaa aatagttcct aaaacaaaca ggaatccttg catcttgatt tggaatcttg
    25861 cccattttgc ccattcgatc cgtgcaacac ctagcacacc aatcaagctg gctgatacgg
    25921 gtgtaaaagc gtccataaaa cctgaaccaa gctgataagc aagaactgct acttgtctgg
    25981 atacacctac taaatctgcc agtggagcca tgattggcat agtcaaagct gcttgtcctg
    26041 aatttgatgt aacaaccaag ttgaacaaac tttggaagat gtacatcgcc caagcaccaa
    26101 tgacagctgg aacaccggtc aacgccgtcc cgatcccatg caaaatcgta ttcaaagctg
    26161 aaggaacaga tgcatcagat cctcccaaga caagaagaat ccctttggcc atacctacta
    26221 cgattgctgt accagctaaa tcagctgcac cagattggaa agaagaagcg atgtcattga
    26281 ctttcattcc atctaatttg aaaagaacag caatgattcc agcaactaat cccatcacaa
    26341 agaattgaga agcgatttct ggaatgtaat agcctttttg agtcacgccc caaacgatcc
    26401 agacaaggac aactaacatc tctaataaga tcaatttatg cccaagcatg aattttttat
    26461 tttcatcaga tgtctttttg atatgagaac ggaaataatc atctgatttg taagtcaacg
    26521 aactttctgg ttttttacgg attttttctg catatatcat catataacca gcggctaatg
    26581 cagtaacaac tacccacata atcaatcgga atgtcgcacc agacaacacg gggattccag
    26641 caatcccttg agccaccgct acgctaaacg ggctcatcca agaggtcgca tttccgactt
    26701 gagatgcgac ataagtcacg gttactgcta cgatcgagtc atatcctaac gcaatgacaa
    26761 atggaaccat caccattgag aatggaatta cctcttctgc catcccgaat gtagcgccac
    26821 cgaatgaaaa agcaaagaat aatagcggta aagctaaacg ctccagtccc ttagttttac
    26881 tgataaacgc atagatacct gcgtcgatcg ctccggtacg cataataata ccaaaagcac
    26941 ctccaacaac aaggatcaat gcagcgatcc ctacagcaga tccatactta tctccagata
    27001 ctagtccttc aaaaacaaaa tttaagaaac caaagccgcc aaaatcatct gttccccata
    27061 ttttggctgt tttatgcaat tttttagaag tatcatagat attttcgccg tactgatcgt
    27121 aaagcacatc atctgtcaag gagatttcat ctagtttttc ctgcgtaaga tttttttctc
    27181 cgtcagcaat gactttttcc aatccttttt ccggaacatc cagtttttca cgttctgctg
    27241 gatgatcttg cagttcttct aattgatcaa acacatacga cttatccagc tcgtaagcat
    27301 aacggaagga atcttgacgt aaaacagtac gagtacttgt ttcgccattt gcatctttgt
    27361 attcaatatc ttgcgtgctg aactttcctg ctggaacgac aaaagtcagt atccatgcaa
    27421 agatgactac ac
//
LOCUS       DACEXQ010000017        26540 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.017, whole genome shotgun sequence.
ACCESSION   DACEXQ010000017 DACEXQ010000000
VERSION     DACEXQ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.017"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..452)
                     /locus_tag="IXH04_000542"
     CDS             complement(<1..452)
                     /locus_tag="IXH04_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1851175.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEII"
     gene            complement(490..804)
                     /locus_tag="IXH04_000543"
     CDS             complement(490..804)
                     /locus_tag="IXH04_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851176.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(792..1421)
                     /locus_tag="IXH04_000544"
     CDS             complement(792..1421)
                     /locus_tag="IXH04_000544"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAQ1851177.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(1428..2144)
                     /gene="pyrF"
                     /locus_tag="IXH04_000545"
     CDS             complement(1428..2144)
                     /gene="pyrF"
                     /locus_tag="IXH04_000545"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAQ1851178.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(2141..3073)
                     /locus_tag="IXH04_000546"
     CDS             complement(2141..3073)
                     /locus_tag="IXH04_000546"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAQ1851179.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(3070..3846)
                     /locus_tag="IXH04_000547"
     CDS             complement(3070..3846)
                     /locus_tag="IXH04_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAQ1851180.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(3900..7085)
                     /gene="carB"
                     /locus_tag="IXH04_000548"
     CDS             complement(3900..7085)
                     /gene="carB"
                     /locus_tag="IXH04_000548"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAQ1851181.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(7090..8166)
                     /gene="carA"
                     /locus_tag="IXH04_000549"
     CDS             complement(7090..8166)
                     /gene="carA"
                     /locus_tag="IXH04_000549"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAQ1851182.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(8171..9451)
                     /locus_tag="IXH04_000550"
     CDS             complement(8171..9451)
                     /locus_tag="IXH04_000550"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAQ1851183.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(9470..10396)
                     /locus_tag="IXH04_000551"
     CDS             complement(9470..10396)
                     /locus_tag="IXH04_000551"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAQ1851184.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(10409..11692)
                     /locus_tag="IXH04_000552"
     CDS             complement(10409..11692)
                     /locus_tag="IXH04_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAQ1851185.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(11706..12245)
                     /gene="pyrR"
                     /locus_tag="IXH04_000553"
     CDS             complement(11706..12245)
                     /gene="pyrR"
                     /locus_tag="IXH04_000553"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAQ1851186.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(12480..13391)
                     /locus_tag="IXH04_000554"
     CDS             complement(12480..13391)
                     /locus_tag="IXH04_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ1851187.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(13388..13852)
                     /locus_tag="IXH04_000555"
     CDS             complement(13388..13852)
                     /locus_tag="IXH04_000555"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAQ1851188.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(13857..14555)
                     /locus_tag="IXH04_000556"
     CDS             complement(13857..14555)
                     /locus_tag="IXH04_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ1851189.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(14542..14928)
                     /locus_tag="IXH04_000557"
     CDS             complement(14542..14928)
                     /locus_tag="IXH04_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851190.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(15075..16745)
                     /locus_tag="IXH04_000558"
     CDS             complement(15075..16745)
                     /locus_tag="IXH04_000558"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAQ1851191.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            16928..17161
                     /locus_tag="IXH04_000559"
     CDS             16928..17161
                     /locus_tag="IXH04_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAQ1851192.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(17260..17691)
                     /locus_tag="IXH04_000560"
     CDS             complement(17260..17691)
                     /locus_tag="IXH04_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAQ1851193.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            17770..18183
                     /locus_tag="IXH04_000561"
     CDS             17770..18183
                     /locus_tag="IXH04_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAQ1851194.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(18221..19369)
                     /locus_tag="IXH04_000562"
     CDS             complement(18221..19369)
                     /locus_tag="IXH04_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ1851195.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(19491..20087)
                     /locus_tag="IXH04_000563"
     CDS             complement(19491..20087)
                     /locus_tag="IXH04_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1851196.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(20287..22929)
                     /gene="alaS"
                     /locus_tag="IXH04_000564"
     CDS             complement(20287..22929)
                     /gene="alaS"
                     /locus_tag="IXH04_000564"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAQ1851197.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            23317..23748
                     /locus_tag="IXH04_000565"
     CDS             23317..23748
                     /locus_tag="IXH04_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1851198.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(23793..25142)
                     /locus_tag="IXH04_000566"
     CDS             complement(23793..25142)
                     /locus_tag="IXH04_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ1851199.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(25212..26375)
                     /locus_tag="IXH04_000567"
     CDS             complement(25212..26375)
                     /locus_tag="IXH04_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851200.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
BASE COUNT         7426 a         5833 c         4673 g         8608 t
ORIGIN      
        1 ttgatgattt catcaaaaga ttcataaaga aggtcaacct tatctgaaat ggctaatagt
       61 tctccttcta cggtttcatc ttttccttcg aacaaccgac ggcgataaac ttcttggaac
      121 tcagctggaa tctcttggtt gatgaattcg tccgtcatct tttcttcaac tgtttgcagc
      181 atccctctta actctttgct tgcgtatttc accggcgttt tgatatcacc aataaaacgc
      241 tctgtgtagt catgattcaa tgatttttca tagagcagtt tccaatcgat cggttgtcca
      301 ttcgcttctt caatgtctcc tagaacttgc gcaatagatg ctacacgata cgaatgtgct
      361 gcaactgtat gctctgtata tttaaaaaat ccaggtgccc ttgtgatcgt ctctaaattg
      421 ttcaatccta aaataaattc attcaacccc ataaaagtta cctccctttg agatttcttt
      481 ttccgaatat catatcatta aataactgat attcgcaaca tttccgctct ttaccatttt
      541 tgaaacagct cccttaccaa aaaatagaga aaatctatat ataatagaaa aaagagaaga
      601 gattattctc ttctttctgc taaccagtct tagaaaaatg aattttaaaa aaatcaaaaa
      661 ctgatcacga tagaagtttc ttttcatacc tgatgaaaag atgcttctcg caatcagttt
      721 ttggattttt gctattccaa ttgatgattc tggagacaca aatcggattt ctctatcttc
      781 atcaagcgcg cttaaatctg ccaattttca ggatcttttt tccattcttt tagcagagtt
      841 aattcttgct catcgatata acgatcttct aaagctgctt caatcaatac agaataattg
      901 gtcaacgtca taagagggat ttcggctttt tcgaaatttg cttttccttt tggcaactca
      961 tacgtaaaga tagccgcaac accaaggata tccgcacctt ctctttttgc tgcttcagct
     1021 gcttccaaga cacttccgcc tgtagaaatc aaatcctcga tcacaaccat cttctgacct
     1081 tcaaatatac gtccttcgat ttggttacct ttcccatgat ctttcgcctt acttcgaata
     1141 tagaccattg gcaaatccag tatttccgct acccatgcgg catgagggat tcctgctgtt
     1201 gcagttccag caatcacttg tacttcaggg taagcttctt tgattttgct tgctaatcct
     1261 tttgcgatct cttttcggac ttttggatag ctcatcgtga ttcggttatc acagtaaatt
     1321 gggcttttga ttccacttgc ccatgtaaaa ggattacttg gatttaaaaa tactgcttca
     1381 atttctaata agtcttttgc gatactttgt tcaacattca ttttaggtta gctccattct
     1441 gtttgaatcg tttgatatgc tttataagga ttagttgact gagtgattgg tcgacctata
     1501 acgataaaag ttgagccgat cttttttgcc tgagaaggtg tgactactct tgtctgatct
     1561 cctgcgactg ttccttctgg tcttataccc ggagttaagc aaacaaattc gtctcctgcc
     1621 atttctttga tcgcttctac ttcccaagca gaagaaacca caccatctaa tcctgctttt
     1681 ttggcacagc tagcataatg gatcacactt ttttctaaag aaacttcgat tagttgatcg
     1741 gcatgcattt cttcttcact tgtagaagta agctgagtga tagctagaag tagcggacgt
     1801 tttccacctt ctggagtccc ctcttctaat cctcgcattg ccgcctccat catacgaata
     1861 ccgccagcgg catgaacaca ggtgagatca acaccaagct ttgccaatcc ccgcattgct
     1921 ttttcgaccg tattcggaat atcgtgaagt ttcagatcta aaaatacatt atgaccagcc
     1981 tcttttagat aacgtacgat ttcaggtcct tcttggtaga acaattccat tccgattttc
     2041 acaaacaatt tttcttcttt cgggaaaaat cgaagaaagt cttctacctc ttgcctagtt
     2101 gaaaaatcta atgcaataat tggtcgttgg tctagttgat tcatcgttca cgttcctctc
     2161 tcacttcttt gattaatctt tccagcgatt cgatgcctag ttcgctcatt cgcacaggga
     2221 gctctttaat tagtttagga cagatatacg gatcggtgaa attcgcggtc cctatagcta
     2281 cagcacttgc tccagccata aacatctcaa gaacatcatc cactgtctgc acgccgccca
     2341 tcccaatgat tggaaggctg gatatacttg ctacttggtg aatcaaacga atagcgacag
     2401 gcttgattgc tggtccggag agtccacctg tttgattggc taaaatcggt tttcttgtct
     2461 taaggtcaat ccgcatacca agaagagtat tgatcatcgt aaacccatct gcccctccag
     2521 cttcgattgc ctgagcgatt ggaacgatgt ctgttacatt tggggacaat ttgacataga
     2581 caggaacaga agaaactttc tttactgctt gtgttaattg aaaagcaaca tcagaatctg
     2641 ttccaaaagc aattccgcca tgtttgacat ttgggcaaga aatgttcagc tcgattgctt
     2701 tcacatttgg tgcttcgcca attttgctac aaacttctac atagtcttct tcacaagcac
     2761 cagcaacatt tgcgatgatg ggtaactcat tgtactcctc taattctgga agaagtttat
     2821 gcataacaac gtctagacca ggattttgca aaccaattgc attcaacatg ccagatggtg
     2881 tttccgccac tcttggcgtg ggattgccaa agcgggcatt tggagtcgtt gccttgacca
     2941 tgatcgatcc aagctgattc aaatcatagt attttccgta ttctttgcca aaaccgaaac
     3001 agccgcttgc cggcatgata ggatttttta attctaggcc aggaagtttg accgctaaag
     3061 gattctcatt catataatta cctctcctat tttaaaaatc ggtccttcgt cacaaacttt
     3121 cacacttttt gttcctgttt catcttccgg cgtatgacaa acgcaagcat agcaagcccc
     3181 tatcccacat gccatgcggg attctaatga taactggaca ttttgatgcg aagaaaacag
     3241 ctgttccact gtcttcaata acccgtttga accacatgca aaaacagctg taggttccgt
     3301 cttcgttcct aacaataaat taccgacatt tccgtgcata ccgtatgttc catcgtcagt
     3361 agcgattctt gtttctccta gacgattaaa ttctttttca taataaacga cttcatgtgt
     3421 ggcaaaacct aaaaaatgcg ttggcttaat tcctttttcg actaactgtt ttgataactc
     3481 atatagagga gggacaccga ttcctccacc gattataaat acttcgtctc ctgtatgaag
     3541 attggacaaa tcaaaaccat tgcctaaagg acccatgaca tctaaatggt ctccggcaga
     3601 aagagtagag aagactctag tcccctcacc ttccacccga taaatgatac ggcaagtatt
     3661 tgtttcgtgg tcgatatgat tcaagctaat cggtcggcgt aatagcaagt ctgcacgcgg
     3721 cacgagaata tgcaagaact gtcctggttg ctgcatttct tttactagat cacctttcaa
     3781 tgtcatttca aaaatctttg gtgccagctg tttttgctga acgatttcca tcatttcttg
     3841 tttcatctgt cccctcctgc aacggttttt cgtctttttg tcttaacatg tgctcgccgt
     3901 taaatagggt tagttgaaaa agcacgggat tccatcactc gaacgatcgc atctgctgtg
     3961 tctaaagaag taaacaatgg aataccatgt tcaactgctt ctcttcggat gatgaaaccg
     4021 tctttagaag catttccccg atctttatca atcgtattga caaccacttg tgttttacca
     4081 tctcttatta agtcgactac atttttttct gctgcttcct cagaaatttt cgctactgtt
     4141 tcaacgacca gaccattttt ttcgaaataa gcagctgtat gtttcgttcc tatcagacta
     4201 tagccaattt ctgtaaaacg tttagctaat tctagtgctt cttcttttgt ttcgtccgca
     4261 atcgtaaata atacggctcc atattcaggc aagtgtaatc cagaagcttc gaatgccttg
     4321 tacaaagctt tgtctaattg tctgtcagat cccattactt ctccagtcga tttcatttca
     4381 ggccctaagt aagtatcaac tttctgcaat ttcgtaaaag aaaagacagg tgctttaaca
     4441 tgaacttctt ttgtttctgg ataaagtccg ttttcataac caagtgtctt tagtttctct
     4501 cccaaaatag ctttcgtcgc aatttgtgcc attggaattc ctgtcacctt acttaagaat
     4561 ggaacagtcc gacttgctcg aggattgact tcgatcacat aaacctcatt ttcatggatg
     4621 acaaactgga tattcatcat cccgacacaa ttcaaaccta atgctaagcg gatcgtgtaa
     4681 tcttcgatcg ttttcttgat cgattcagat aatgtttgag gtggatacac agccattgaa
     4741 tcccctgaat ggacaccagc acgctcaata tgctccatga tccctgggat gagtactgtt
     4801 tcaccgtcac aaatcgcatc cacttcgcat tcaccaccaa gcaagtaacg gtccactagt
     4861 actggatgtt ctggagatgc tttcacagca tgttccatgt aatcttctaa atctcgctgg
     4921 ttttcaacga tttccatcgc acgtccgcct aatacatagc ttggacgaac aagaactgga
     4981 taaccaattc tcgaagcgat aaccacggct tcttctttac ttgtagcagt atcccctaaa
     5041 ggttgaggaa tatccaactc tttcagcgct tgctcgaata aatcacgatt ttccgcacga
     5101 tccaagtctt cgatcgttgt ccctaagatt tttacaccag cagcagtcaa aggttcggct
     5161 aagttgattg ctgtctgacc accaaattga acgattactc ctatcggctg ttctagctcg
     5221 atcacattca tcacatcttc gaaggtcaat ggctcaaaat ataatttatc cgaaatagaa
     5281 aagtcagtcg aaactgtttc aggattgctg ttcatgatga ttgcttcgta tcctgcctgc
     5341 tggattgctt tgacagaatg cacagtcgca tagtcaaatt caaccccttg tccgatacgg
     5401 ataggaccag atcccagtac tagaattgag ggcttttctg atcgtgaact ttcattttca
     5461 tattcatagg aactataaaa ataaggtgtt tttgattcga attccgctgc acaagtgtca
     5521 accatcttat atacaggaat caaatcattt tcttttctca tctggcgaac ggcttcttcg
     5581 tttatttccc acagctccgc cacttttttg tctgtaaaac cgttttgttt cgctgttttc
     5641 aatatcttgt catccaacgg ctgctgtttc aactgctgtt cgatttctac gatatgcaac
     5701 agtttatcta aaaagaataa atcgattttt gtcaaatccg ctaattcttc tatggtgtac
     5761 cctcttctaa tagcttcagc tacataaaac aggcgatcat cttgtgctcg aaccactttt
     5821 tggattaggt catcgtccgt gatgtctgcc aactcaggaa gttcgatatg gtgtgtaccg
     5881 atttctaaag aacgcacagc tttcaataaa gactcttcga tattccgacc gatagccatc
     5941 acttcaccag ttgctttcat ctgcgtgcct aaaagccgtt ctccgttttc aaacttatca
     6001 aacggccagc gaggaatttt agctacaaca taatctaatg caggttcaaa ttctgcaaaa
     6061 gtcgttcctg taacaggatt tttcatttcg tctaatgtta atccaagcgc tattttagca
     6121 gctagcttag cgatcggata gccagtcgcc ttacttgcta acgcagatga acgagaaaca
     6181 cgcggattga cttcaatcac ataataatta aaactatgtg gatcaagagc gagttggaca
     6241 ttacagcctc cctcgatctt aagtgctcga ataatcgaaa gagaagcatc acgaagcatt
     6301 tgatattcat ggtcagacaa tgtttgacta ggggcaaata cgatagaatc acctgtatgg
     6361 atacccaccg ggtcaaaatt ttccatattg cagacaacga ttgcattatc agccgaatct
     6421 ctcatcactt catattcaat ttctttaaaa cctgcaatac ttcgttcaat caaacattgg
     6481 gtagcggggg aaagacttaa tccattttct gatacgatac gtaattcttc ttcattttca
     6541 cacatacctc caccagttcc gcctaaagta aaggctggac gaacgatgac aggatagccg
     6601 attttatttg caaaatctac agcttcttct actgtattga cgatttcaga ctcggggatt
     6661 ggctgattca attctttcat caactgtttg aatagatcac gatcttctgc ttgatcgatc
     6721 gcagaaagct ttgttcctag aagttctatg ttcaattctt ctaaaatacc tgatgctgcc
     6781 agctccattg ccatattgag gcctgtctgt ccgcctaaag ttggaaggag agcatctgga
     6841 cgctcttttc gaagaatcct tgatacaaat tcaagtgtga tcggttcgat atatacttga
     6901 tctgccactt ctttatccgt catgatcgtc gcaggattag aatttactaa aaccacttca
     6961 tatccttctt cttttaaggc aaggcaggct tgtgttcctg cgtagtcaaa ttctgcggct
     7021 tgaccaatga tgatcggacc agatccaatc accataattt tcttgatatc tgtacgtttc
     7081 ggcatctgtt tactgctcct ttcctgcgtc catcatttcc ataaattcat caaataaatg
     7141 tagtgcatca tgcggaccag gggcagcatc tggatgaaac tgcacactga aagcgggata
     7201 atctctgtgt ttgactcctt ctactgttcc gtcatttact tctacatgag ttacgatgag
     7261 tttttctgga tcaatcgtgg cttcatcgac tgcatagcca tgattttgag aagtaaaatc
     7321 aattcttccg gtcgctactt ctcgtaccgg gtgatttaat cctcgatgtc cgaatttcat
     7381 tttataggta tctgcaccgt tggctaaagc aaataactgg tgaccgagac aaataccaaa
     7441 aatcggtact tttccttgga tttttttgat catttcgatt gcttctggca catcttttgg
     7501 atcaccaggt ccattcgtca gcatgactcc gtctggtgat agcgctaaaa tcgtctcggc
     7561 atctgtgtta tagggaagta ctgtgaggtt acagtttctt ttactcaatt ctcgtaaaat
     7621 actatgtttc aagccgaagt caatgacaat gactttccga cctgttcctg gacttggata
     7681 aggtttgtta gttgagactt gctgcacttg attggttgct agaacggttg ctttcagctg
     7741 atcaaatgca tgttcgaagg aatctccagc atcaactata ctagctttca tcgttccgac
     7801 ttcacggatt ttgcgagtaa gggcgcgcgt atcgatacct gatattccag gaattccttt
     7861 ttgtttcaaa aatgaatcta gcgtcatttg gtttcgccag tttgaagcta cacgtgcatg
     7921 ttcttttact actactcctt tgcatgttgg agaaatcgat tcataatcat ctctatttac
     7981 cccataatta cccaccatag gataggtaaa tgtaatgatt tgaccgttaa aactttgatc
     8041 agtgatagtt tcttgataac cagtcatact tgttgtaaaa acgatttctc cgaagacact
     8101 agcatctgca ccaaaagcct ttccttcgaa aactgttcca tcttctaata taagcaaacg
     8161 ttccatactt ttattctccc ttctgccaca caagttttcc gttgacaaaa gtcatttgtg
     8221 tttcgccttt tactttccag ccgataaaag gtgtattctc cccttttgat aaaaagtctt
     8281 ctttatcgat cgtgcaagtt tgtgtaatat caaaaactgc tacatcagcg ggggcaccaa
     8341 ccgttagcgt tcctgcattc aaaccaaaaa tctcagccgg tttaactgcc aaccaattga
     8401 tcacttgttc caacgtgaat cgaccagttt ctacaaaatg agtatagatc agttgaaacg
     8461 ctgtttcgct tcctacgata ccaaatggtg atttcatgaa actttggctt ttttcttcta
     8521 atccatgggg ggcatggtct gtagcgatgc aatcgatcgt accgtcaagc aagccttcga
     8581 tcaaagcttc tctgtcctcc cgtcctctta gcggcgggtt cattttccaa aagccgaaat
     8641 cttcgggaat atcttcgtcg atcaaaataa gatgatgggg tgaaacttct gctgttacgt
     8701 ggattcctgc tttttttgcg tctctgatta cacggacact ttctttagta gaaacatggc
     8761 aaacatggta atgaacacca gttgcttcag ctagcaacag atctctagca atttgagaag
     8821 actcggttac gcttaagatc ccgggaagac caagttcttc tgccttcttg ccagcgtgca
     8881 taacgccgcc aaataataaa gattcatctt ctgtatgggc tactagggct ttattgtttt
     8941 ttgcagcttc tttcatagcc agatacatcg tcccagctgt ttggacacct acaccgtcat
     9001 ttgtgaaagc aaaagcacct tcttcaatca gtgcttcttg atcaactaat ttctcacttc
     9061 gcagattttc tgtgattggc gcatactgca ataccttcac aactgcatct tttctaatga
     9121 tgtcatatac ttgtctcagc ttttctgccg tatcaggtac aggatttaaa ttcggcatcg
     9181 cgcatacagt agtgaaaccg ccacgagcag ctgagcgagt cccagcttcg atcgtctcct
     9241 tgtatgtaaa tcctggctct ctcaaatgaa catggacgtc cactaaacca ggtgtgatca
     9301 attgtccctt tgcatcaaac acttcatcaa attcggcttc tgagaatcct gtaccgattg
     9361 ctttgattcg cccatcttct atccaaattt ctgctggtgt cgtcatattt tttcgtgtat
     9421 tgatctgacc gtttttaatg agtgttttca ttttttcatc ctcctataat tacgcttttc
     9481 catttagcac tgcttctaag attgccattc tgacgaatac tccattgctc atttgctgaa
     9541 cgatccgcga ctgtaatcct tccactaatg aatcagctaa ttctacatca cggttaactg
     9601 gtgctgggtg cataatgatt gcatgttttt gcaacttctt cgcacgctca actgttaacc
     9661 catgttcctg atggtagcct tcttttgaga aactttcttt tccatcatgg cgttcgtgtt
     9721 gcacccgaag catcatcatg acatcaactt gatctagtag ttcatccaat ggcatataat
     9781 gtccgtacac ttcgaattca tcgtcgtacc attctctagg acctgagaag aatacttgtg
     9841 cgcctagacg ttttaacatc tgcatattgg atttagctac gcgagagtga gtgatatcac
     9901 ctacgatggc aactttcaga ccttcaaaat gaccaaattc ttcataaatg gtcatcaaat
     9961 caagcaagca ttgggttgga tgctgtccgc ttccgtctcc tccattgata atcgaacact
    10021 ggattgtttt actttgtatc agttcgtcat aatagttctc atcaccatga cggataaccg
    10081 ctacgtctac gcctaatgct gacatcgtca gtactgtatc gtatagtgtt tccccttttt
    10141 ggacagagct tgtgcttgct tcaaattcaa tcacttctag ccctagtttt ttctccgcta
    10201 catcgaagct tttatgtgtg cgagtactat tttcgaaaaa gaggtttgta gcaaagtact
    10261 ggcgcttttg cggagtccaa ttagcacctt gcttgaattc ctgccctctg cggatcaagc
    10321 ccataacttc ttgatcagta agtgcctcaa cggtcaataa atgctttaag ctgatacgtt
    10381 ctgatttgat gatcataatg tttcctcctt agttttcttc gcttcgtgaa gtttctggta
    10441 agataagatt taaaatgatg ccaagtacag ttgctaatgc cattgccgac aatgtaaatg
    10501 tgcctacttc aaagacgagt ccgccaattc ctactactaa gataactgaa gcaatcaaca
    10561 gatttttctt tttatcaaaa tcgattttgt tctcgattaa aatcttcatt ccacttgcgg
    10621 caattactcc aaataagata aaactgattc ctgagatgac tggtccaggg atacttaaaa
    10681 tcaaagcgct taattttcct acgaatccta aaatgactgc aaatactgca gctccaccga
    10741 tcacaaacac gctatggaca cgtgtgatag ccagaacacc aatgttttca ccgtaacttg
    10801 ttacaggagg acctccgaca aaggctgcca cgatttgtgc taatccatca cctgtcaacg
    10861 tcttatgaag acccggttct tcaaagaaat tacgtttagt tagtttattc aataccataa
    10921 gatggccgat atgctctgtc atcgtcacaa aagcgatggg tgccattgtt gtgattgctc
    10981 caagatatag ctttggttca tattgtacaa agggaatctc aaagttagga acagcaaacc
    11041 aggcagcgtc tacgattggc ttagtatcca cgattccaaa cagcaaggct acgatgtatc
    11101 cgctaataat acctagtaaa atcggaatca atccgagaaa acctcttagc cacatgttga
    11161 agaaaattgt taatcctaat gtgatcagcg ctacagcaat atacttgaaa tcatacgtgc
    11221 cctgattata catagcattt gttgctgcat ttgccgcaag tcccaacccg ataaccatga
    11281 tgactggacc gacgacgatt ggaggcaaga ttttatccag ccatgctgaa ccgatctttt
    11341 tgatgatcag cgcaacaatc aaatagacta atccagttgt gactgctcct tgtgcaatcg
    11401 ctggataacc atctgttttc atcaaaagct gcatcgctgc aatgaaagcg aagctgcttc
    11461 ctaagtacgc aggtatcttt cctttagtag taaataagta caccaatgta cctagtcctg
    11521 aactgactag cgcaatacct ggatcgatac ctactaagat ggggactaac acggttgctc
    11581 cgaacatcgt gaacaagtgt tgtaaactta atcctaccca atgtcctggg gttgggcgat
    11641 cgtggatatc taacaccacg tcatcattat gaaatccttt tttctccgac atgtttctct
    11701 ctcctctaat cttcctcttt caaaatcatg atgcgatctt gtccatcaag ctcttgcatc
    11761 tctactaaaa tctcttccgc ccgagatgtt ggaatgttct tcccaacata atctgcacgg
    11821 attggcaatt ctcgatgccc acgatcgacc aggactgcta atgaaatttt tcttggtctg
    11881 ccaaaatcca ttactgcgtc cattgctgct cgaatcgttc gtcctgtata caaaacatca
    11941 tcaatcaaaa taacttcttt tccttctaag gaaactggga tgtcagaaga atgaagttcc
    12001 ggttcttctg gattttcttt tttgtcatct cggtataaag tgatatccag ttctcctaca
    12061 ggaatatcga tatcttctag ctgcttcagg cgttcggcaa ttcttgatgc aatgtagatt
    12121 cctcgtgttt tgatacctac caatacgata tcttggatac tatggtttct ctcgataatc
    12181 tcataagtaa ttcgagtcaa tgctcgcttc atggttacct gatctactac ttcttttgct
    12241 tgcattttca ttccccctac ttttttgcat aaaaaaactc ctgccctaag aggacaggag
    12301 gaatgtttag actgagcgta tactgataca ctgatagcta tactatgtca atgagcaatc
    12361 tacttatggc gcaccatacg tatccttgct cccacccttc ttagcctcac gggactaaac
    12421 ttaaaggaat tctattaagt tttatgcttt cgttgttaat tactataccg ttatctaaat
    12481 taaaatgcaa tgttttttct taatttttct aaagtttttt caaaaacttc tggaagaggt
    12541 gcttcaaaga tcatcttttc gtttgtagtc gggtgcgtaa aacctaacat ttgcgcatgg
    12601 agaaactgac cattgccttt caaagttttc ttcggtccgt atactggatc tccagctaac
    12661 ggataaccga tatacttcat atgcacacgg atctggtgtg ttcttccggt ttctagtttt
    12721 aattcaacca gagtaaatcc ttcgaaacgt tccaatacag taaaatgtgt gacagccgac
    12781 ttcccatctt ctcttatcgc ctgcattttc cggttttcct tcgaacggcc gattggcgca
    12841 ttgatggttc ctttttcgtg aggaatctct ccatggacta aagcaacata tttgcgcaaa
    12901 gaggtcttat cttttaattg ctgagctaat gattcgtgtg cctggtcatt tttggcaacc
    12961 attagcagtc cggaagtatc tttgtcgatt cggtgaacaa tacctggacg aataacgccg
    13021 ttgatcgtag aaagatcctt gatatggtac atcagcgcat tgaccattgt gccatgcgta
    13081 tgacctgctg aaggatgaac gaccattcct tgaggtttgt ttatcaccgc tacatcttca
    13141 tcttcgtaaa cgatcgtcaa cgggatattt tctgggacca catctaatgt ttctggttcg
    13201 gggatcaaga cagtaatctc gtctccttgt tttactttgt aatttgcttt gactggctgt
    13261 ccattgacag aaaccgctcc atttttcagc cacagctgaa tttgcgaacg actgtaatcg
    13321 gtgaataaat tagtcaatac tttatcgatc ctaccagatt catggtgaat cgtcgtttta
    13381 agttctgtca tttttttctt tgatcctttt catctaaaat aatataaatt aacatacaaa
    13441 tcactccaca tacaagtgtc acatctgcta cgttaaaaat cggaaagctc ataaaatctg
    13501 tctggaacat atctactaca tagccaagac gaatacggtc gataaaattg ccaatcgcac
    13561 cagctagaat caaactcaat ccaatcatga accagcgatt acctttttca cgatttttaa
    13621 tcatcaatgt gagaatcaca acgactgcaa tgagtgtcac tatagtaaag aaccacatct
    13681 tcccttctag catactccat gcggctccag tgtttcgaat atgattcagt gacaaaatac
    13741 ctggtataag atttttcgtc tctccaagcg ctagattatc agtgatccac catttgacca
    13801 actgatccac tcctactagc aaagcgctga atatccaata aataattaac aaaaaatcaa
    13861 tccttttcta tttgtttata aatatcacga ggtcgtacga tcccgagcag attaccatcg
    13921 ggattcccat ctttagtgat aagtacggct tccaaagtcc cttgctcacg aaacatcgct
    13981 tcaacctcgt aaatatgtgt tttagctgga acaaaatgat aatttgtgta ctttccgttt
    14041 tggatgatca aatccaacgc tcgtttattt gttaaatcaa ccggaccttt ttggctttct
    14101 tttgctagcc aaaacccgat attacgtaaa gtaatcaatg ctacaaattt tcctttattg
    14161 tataacggaa attgtgaata ccttttttct gctactatct ttaacagttc aagtaacgga
    14221 agatcttgtt caaaacctgt tacacgtttt gcaaatcgag gcagcacggt ttcaggctga
    14281 atcagttctt gttcgatcag ctggattcgc ttgactgtcc aatcatttgg ctctgcaatg
    14341 acaaaatcga ctgcgatccg atcatggacg atcgcattcc ttaactgagc aagctgtaaa
    14401 agatcatctt cgtattttct gatcattaat tgttttcttt gagctaggcg tctgacaagt
    14461 tcggtaaatc ccatatttcg tgcatttccc atctcgtctc tcatccattt ttcgattcga
    14521 ttgaaactga ttaaaaaaag atcagagttt gccataattt tcctccttga tcaaatatgc
    14581 ccggtttttt atatagaaaa agaacgcaat caacacaata actgcaaaaa aactgaaaaa
    14641 taaattttgt aggaaaacgg cccccaaccc agttgctaga aataaaaacg gatagatttg
    14701 aagaatcaag taattcattg tgatcacata agcaggcttt gcttctttca ctgtataaac
    14761 ggggcttttt agccaataaa aggtgacctg catcgtaaag ggcacttcca gatcaagcgt
    14821 tatcttttgt tcatgactga gaacactgcg tttttcattg ttgattctta gctgaatcga
    14881 gctacccata ccgtaaaaac cggtttttct tataaatgtc gttttcatca taccctccta
    14941 ttatgcttta gtatagctca aaatacaata aagaaaaaga atgatcctag tgtaaacaaa
    15001 attaattccc tatcgtctaa aaaaagaagc tgtagtcaaa atacagctcc ttcgatggta
    15061 gattataatc aagcttaaaa caatcccaac acttctccgt catttgtcac gtccatattc
    15121 aatgcagcag gttttttagg caaaccaggc atcgtcataa catctccagt caaggctact
    15181 aaaaatcctg cccctaattt tgggatgatt tcgcggatcg tgatggtgaa atcttccggt
    15241 gcacctagca gtgaaggatc atcagaaaat gaatactgtg tttttgccat acaaatcggc
    15301 agtttgtccc atccattctt cgtaaactct tctatttgtt tttgcgcttt tttgctgaaa
    15361 acaacaccat ttcctccgta aattttcttg acgattgttt ctgtcttttc ttgtatcgtt
    15421 tcttctaggc ggtataatgg cttgtaatta gcttcttttc tatcaatcaa tcgtactaca
    15481 gcttttgcta aatcaacgcc gccttctgct ccatctgccc atacactcgc tctcttggca
    15541 agaatcccct gattttcaca caagtgctcc aacagtgtga gttcattgtc tgtatctgtt
    15601 acgaattcat tgattgcgac gatcacaggt agctgatatt gttccatatt acgtatatgg
    15661 cgctttaaat ttgcaaaacc tactttcaaa gcatcaagat tctcattttt aagttcatct
    15721 tttttcattc cgccatgcat tttcaatgca cggacagtgg cgacaatcac cacagcatcc
    15781 ggcgcttttt tcaagttagg tactttgata tctaaaaatt tctctccgcc aagatctgca
    15841 ccaaatcctg cctctgtgac aacgtaatct gctaatctca atgctgtctt ggtagctaaa
    15901 atactattgc agccatgagc gatattggca aacggtcccc cgtggacaaa agctggtgtt
    15961 ccgtaaattg tttgtacaag attcggcttg attgcatctt tcagcagtaa tgccagagcg
    16021 ccttctgctt tcaagtctcc agcagtcaca ggctgacggt caaatgtata gccgacaacg
    16081 atccttgcaa gacgagcctt taaatcttca aggtctgccg caagacacaa gatagccatg
    16141 atttcgctgg caactgtgat atcaaaccca tcttctcttg gaacaccttg gattggccca
    16201 cccaagccaa caattacttt acgcagttca cgatcattga gatcgactac ccgtttccaa
    16261 atcactcggc gggcatcgat gttcaattca tttccttgat gaatatgatt atctaaaaga
    16321 gcagataatg cattattagc tgttgtgata gcatgcatgt caccagtaaa atgcaagttg
    16381 atatcttcca taggcaatac ctgtgcatat cctccgcctg cagcaccacc tttgattccc
    16441 atcacaggac caagtgaggg ttctcgcaat gcaataacag attttttatt gattttattg
    16501 agcgcgtctc ctaaccctac agtgatcgtt gactttcctt cgccagctgg tgttggattg
    16561 atggaagtaa ctaagatcaa atgcccctca gcgttatcct ttgatcgatt gattccagaa
    16621 aaattgattt tcgccttata tttcccatac agttccaaat cgtcttcctt aagatctaat
    16681 ttactagcca cttcaacaat cggttctagc tctgtttctt gtgaaatttg cagatcattc
    16741 ttcatttgat tacccctttt cacgaacttt attcaacaaa atatcttctt tgttaagata
    16801 tgccaattat accttttttt attcagaaaa aaaacataaa attacaggtt tttaatttat
    16861 ttgacgtact tataaatact ctttataatg aaggctatga tcagacttta gaaaggtcgt
    16921 gagtgctttg accacaggaa ttgtaaaatg gtttgacaac aaaaaaggat acggctttat
    16981 cagttatgat gacacagaag aaatttttgt acattttact gcgattgaag aagagggatt
    17041 caaaactttg gaagaaaatc aagtggtcga atttgagatc atcgaaggga atcgaggaac
    17101 acaagctgcc catgtcaaga aaggcaacgt tcagactacc gatactagta tgcatgaata
    17161 gataaacaaa tagagtgaga cgctaaacgg gatgaaccgt acgtctcact cttttctttt
    17221 cctgtaaaaa ttcgtttttt taggttctga tttttaaaat tagctttttc tttttttcgt
    17281 ataaggataa ggaattgatt ctgatttggg tccgtttttt tcaaaatgat caaaaaggac
    17341 tacttgatct ttttttcgca tcatgaaaac agatggggct ttctcatctt gcaacatcat
    17401 ttgcacccca gtattagttg gcgtcaaaga tcgaatctgt ccgacagata tttctcggtc
    17461 tttccaaaaa gcagcataac gtataatcac agagtttgat tttaaaataa aagaacgccg
    17521 caaacctaga aatagaaaaa caagaaaaat acctaaaaca aaattgcttt tccaataagg
    17581 acctgtgttt tctaaagaaa gaatcagact aaaaaacaga atgatcaagg tcaatgacca
    17641 atagatgatc gtatgtgcca gttcaggctg ccatcgaata ttttttttca ttctctaacc
    17701 tctcaccgat tcagtaatga tataatctta ccataacatt agtttgaaca aaaaggagaa
    17761 tctcttttta tgttaaaagt atacattgat gcctctacaa aaggcaaccc tggtcctagt
    17821 ggaggtggaa tcttgattgt ctatcaaggt aaacaagaac agctatctat cccgctttct
    17881 atagggacaa accatcaagc agagttcgaa gtctttttaa aaactttaga atttctaaag
    17941 aaaaacgact tacagaatga aacgattttc tgtttttcag acagcaaaac actagtttct
    18001 acggtcgaca aaaacgcaac gaagaacgag ctcttccttc cctaccttca aagaatccaa
    18061 gaacttctat ccgaattttc tttattgatc ctgcaatggt tgcccgaaaa caaaaataaa
    18121 ggagcagata atcttgcacg tcaggctctc cataaacaat taaaagaaaa aaagactctt
    18181 taaattaaaa actttaagga gtctttttat agtttctcat ttaatataaa agataattgt
    18241 gatcaaacgt aatttccaat tggtaatctc cgattttttc ttctatttga ctacgtaaga
    18301 ttctttctag ttctgtatca acaaactgga atttctcagg gaccactaca tcaaattgca
    18361 gcgtttttcc ttgatgaatt actctaaaat catgaatatt aagccctgta cctgtttcaa
    18421 gaagaatttg ctttaactgc cgcaagatat tatagtattt ttcgttatca acaggcattg
    18481 gatccaagtg acagacaagt tccacatcca aagacttttt aaagtctttt tctatcagat
    18541 caataatctt atgagccaca ttcaaattca ttgattcatc tacttcgatg tggactgatg
    18601 caaagcgttt attgggtcca tagttatgca ccaataaatc atgatagcca agtatatcat
    18661 cgcaagcatt taaatgctct tccatttctt caatctctgc tggcgttggg cgacttccta
    18721 atagttcgta tacaaaattt cgaagcatcg tcaatccact ataaaggata tataatgcta
    18781 aagcaaaacc tatatatcca tcaatacgcc atccagttac ccattcgatt gctgctgaaa
    18841 gcaatacagc cagtgtagta aagacatcat tataactatc tgttgctgta gctctcaatg
    18901 tttcagaatc aatggattgc gcaataacct gatacatttt gccttgtgca aatttcaaaa
    18961 agatggaaat gattaggaca aaaaagacga tgggtgttaa agagacattt tctggatgga
    19021 tgattttttc agcagaagac tctaaaaatt gaaatcctac ataagtaatg atgatcgaaa
    19081 caaataaccc actgatatat tcgaagcgtt cgtgtccata aggatgttcc tgatctgctg
    19141 gctttgcagc aattttaaat ccgatcaaag tcaatatgga tgaagcagta tctgatagac
    19201 tgttcactgc atcagccata atggagacac ttcctgaaaa aagcccgatc attagcttag
    19261 agacaaacaa gatcagattc gtaaccaatc ctaagatacc cgctagtact cctttttttg
    19321 tccgctgttg cggtatttgt ttcttttttt cttcttgttt cttagccaag ttttttaccc
    19381 cttctagttt gcttgttgct caggtaaaaa gatggagagg acgattgttt tcctctcctc
    19441 tcttcctttt tctatctatc gtgcgtccta ttactattaa aagatggtca ttattctggt
    19501 ttcaacggtg ttgctgcaaa taacgctttt gctagtaatg gtgttatgat tcccgcacct
    19561 attgcttctg gtatcccatt gattgctgca acccctgcta aggttttaaa taaaagagca
    19621 gagtctacac cataggcatc tgccgttgca cctgtataaa ataatcccat gaatcctaac
    19681 actaagactg tattgaccaa tgaacccagt acccctgcaa aggctgagct gatcgttatt
    19741 gatttatttt tttgataaat ccagtggaaa accaagcctg ctactaagcc aacaagtact
    19801 ctgggaacta ccgagataat cggattcgtg aacactaatg tatcaaatgg cgttgttggc
    19861 atggcatagg cacgaacaat tgtagcgatt ccccaaaaca agccaatcac catgccatct
    19921 tttgggccta atactactgc agcaataatt actgtgatat ggataattgt taaactaatg
    19981 aatcctaatg gaataaaccc cagccaagga accatcgctt gtacgatgat gattgctaac
    20041 aagatcgcac gtaagactag tcgaaaggtc ttatttctac tattcatttt ttcacccctt
    20101 tttatttata ctaatcgtaa aaatttctgt cactttcgtc aactaaagac catccgaatc
    20161 cgaccatttg attagatgcg ccatccaatt gaaaaaagta tgagggcaag acttgtttgt
    20221 cttcgccccc ataatcatgt ttcgttcaat gtttgaactt ttgtttttct gtcaaacgta
    20281 aacgccctat tgttgcgcaa cccattcttt cgcagcttta agtgcatctg gaataccagc
    20341 aggatttttc ccgccagctt gtgccatgtc tggacgtcca ccaccgccac cgcctacttt
    20401 cggtgcgatt gctttaatca agtcgccagc ttttagtcca gcttcatttg attctttcgt
    20461 cattgccgct agcagactga cttttccttc ttgttcgttt gctaaaacaa gaatatctga
    20521 aatatttttt tgtttccatt ggtcagctaa ttgacgcagc tgattcatat ctttcacttt
    20581 tacttgtgct gcaatgattg tttttccatt gatcgtttct ggatctttga atacatcacc
    20641 agcttgttga ttcgctaact tgctagctaa ttgttcattt tccttttgta gctctcttac
    20701 ttgctgttgc aattgttccg cttttgaaac aacttctttc aattgaggtg atttaacaat
    20761 tgttgctact tgttttagtt gtttttcttc ttcttcaagt agttcgtacg cttctttact
    20821 tgttacagcc tcgattcgtc gcacgcctgc accaattcct gattcagaaa cgattttgaa
    20881 gattccgatg ttttctgtat ttgctacatg tgtgcctccg cacagttcga ttgaccagcc
    20941 gccaatattt actactcgaa cgtcatgtcc gtatttttca ccaaacaaag ccattgcacc
    21001 catgtttttg gctgtatcga tatctgtttc aattgtttca actgggatag cttcccagat
    21061 tttttcgttg acgattcttt ccatttcagc taattcatct gaagtgattt gaccaaagtg
    21121 ggtaaagtcg aatcgtaaat gtccaggagc gactaaagaa ccagcttgat ttgcatgctc
    21181 gccaagtact tctttcaatg ccttgtgcag tagatgggtt gctgtatggt ttttgatgat
    21241 ccgattgcgc atcgattgat cgatcaatag atgataggtt tgtccttcac tcattggtgc
    21301 ttttacttct acggtgtgca taaattgacc attaggcgct ttttgaacat ctgtaacaac
    21361 tgctacgatt tcgcctactt cattcatgat ccatccttga tccgctactt gcccacccat
    21421 ttctgcatag aaaggtgttt gatcaaagat catttgagct tcaccttctg cgacttcatt
    21481 caaaagagac tcacctttaa cgatgaccaa taattgacta tctgattctt gttctgtgta
    21541 tccaatgaat ttactttcta ctttgatatc tgtcaataaa gcagattgta cccccattga
    21601 tttttcagta ctgcgggcag agcgagcacg ttcgcgttgt gcatccattt ctttttcaaa
    21661 accagcatga tcaactttca gtccttcatc ttctgccacc tcttccgtta attctaccgg
    21721 gaagccatat gtgtcgtata gtttgaagat gtcttttcct gctagagtcg tcccgttttc
    21781 gttttttact tcttggatca cttgatttaa gatttccaaa ccttcattga ttgtttcatt
    21841 gaaacgttct tcttctgtac ggataacttt ttcaataaat tctttttgtt gaagtacttc
    21901 aggataataa cttaccatga tttccccaac aacagggact aaacgatata agaatgcttg
    21961 ttcaatacct aattttttcc catgcatgac agcacgacga agcaaacggc gtaagacata
    22021 ccctctccct tcattcgatg gcaatgcacc gtcgccaata gcaaatgaaa gggcgcgaat
    22081 atgatctgcg attactttgt aagaaatatc tgttactggg ttttcaccat attttacttg
    22141 tttgcttagc ttttctacgg cgtggataat aggcataaac aagtctgtct caaagttcgt
    22201 tgacgcatct tgaacgattg agaccatgcg ttccaatccc atccccgtat caatgttttt
    22261 atgaggaagt ggttcaaatg tgttatcagg cttatggttg aattctgaaa atactaagtt
    22321 ccagatttct aaataacgtt cattttctcc tcctggatag ttttcaggat cgtcttctgc
    22381 taaatcgtta taagcttctc cacggtcata gaaaatttcc gtatccggac cacatgggcc
    22441 tgcaccgata tcccagaagt tgtcttctac atcaataatg tgatctagtg gcaggccaac
    22501 ttcttcatgc cagatgcgtt ttgcttctgt atcttttgga taaactgtta cataaagttt
    22561 ttctggatcg aatgccatcc attttggatc agtcaaaaat tcccatgccc agtggatggc
    22621 ttcatttttg aaatagtcac caatcgaaaa attacccagc atctcaaaca tcgtatggtg
    22681 ccgtgctgtt ttaccgacat tttcgatatc atttgttcga atagattttt gggcatttgt
    22741 gattcttgga ttttctggca caactgaacc atcaaaatat tttttcaatg ttgcaacacc
    22801 agagttgatc cacaataagg ttggatcgtt cacggggatc aaagacgcac taggttcaat
    22861 cgtatgtcct ttagattgga agaaatcaag aaacatttgt cgaacttgtg cactacttaa
    22921 ttgtttcatg tcattacctg ctttctataa attaaataca aaaaaacacc atcacagcca
    22981 aggacgaaaa ctcgcggtac caccttgatt gcaatgatgt gtaatcatta cctcttaaac
    23041 tcgataacgc tgagctgcgt cggtatttct accaaaatag aaaaagggag caatttgatt
    23101 agtgactatt cttttttcag ccaagaaaga attttctgtg agtcttaaaa aacaaatcat
    23161 atccttttcg tacttattta gtatacgcat tctattgatc actgtcaata tctcaatttt
    23221 tattttcatt tccttttggt catactccac gatttttggt acgataagaa taaaggaatc
    23281 aatacatctc tgctattcat gacaaggagg aatatgatga aaaaaatagt tggagcggat
    23341 gaatggatac gcgcagctag ctgctttttg agatatcaag tttttgtatt agaacaaggg
    23401 attcctgcag aactagaatt cgacggaaaa gatatcccgc aacaaaccta ttatctattt
    23461 cttgatcaaa aaagaccagt agctactttg cgttatgaaa agaaaacacc tacttgcttc
    23521 aagccggaac gattttgcgt agcaaaagaa taccgaggaa aagggttagg cggtcagttg
    23581 cttgaagctg ctgaaaaacg ggcggtttca gaaggttgtc agactagtta tgtagtagct
    23641 gaaatgacgg ctgtagattt ttatgaaaaa caagggtatc atacttgcac agaacctttt
    23701 attgaagatg aagtactttg tatcggaatg aaaaaaagtc tagcttaaaa acaagagatt
    23761 gtgacacgcc ttttgtcaca acctcttgtt ttttatttat gtgaattttt cttattttta
    23821 cgagcagtac ggctttgctg tctgcgttcg attttgcgtt cttttttatt tttttctgca
    23881 attgcccaat cgattttttt cttataacca ggcttgatct ttttcttttt cttcttcaca
    23941 agaccaatca gcgtgggatc taatacatct tttgattttt ctcgtttggc acgtctatta
    24001 cgatcgtatg ttttgacgat ttccccgttt ttgatttctt ttggttcaaa tgagacgccc
    24061 aatttttcta tcgcatcaat ggcttgctca tcatttggcg aatataacgt aatggccgtt
    24121 ccttttaagc cattacgtcc agtacgtccg acacgatgga taaagaagtc tagatcttct
    24181 ggaatttctg cattaataac atgagagaca ccttcgatat cgatccctct agcagctaaa
    24241 tcagtcgcta caacatattg atactccaga ttctgtactt gtcgcatcac tcgttttctc
    24301 tctcttgggg tgatgtctcc atgaatcttt gctactttca agccttggtt tttcaagtaa
    24361 tcggcaattt catccacatg ctgttttgta ttggcaaata cgattgctaa ataaggatga
    24421 ccaattgtca gcagttgata aatcatttga ttctcatctt taccttttgt tgaaatcagc
    24481 caattttcaa tgtcttcaga aataactgct tttggtttga tttcttcaat gattggattt
    24541 tccatgtatt ttcgtaagaa tggacgaagt ttttctggaa tagtggcaga aaaaactaaa
    24601 aactgcagct tttctggtaa tcgacttgca atttggtcta cttcatttaa aaaacccata
    24661 tccaatgtca tatccgcttc atcgacaaca aatgcaaatg cggtgtgaat cttcagcgct
    24721 tgttcattca tcatgtctaa tattctacca ggcgtgccta ctacaacctg tggctgttgg
    24781 tgatgcagac gatttaattg acgctgtttg tctgtaccgc cgacaaaatt cgagacacgg
    24841 atctccggtg tagaaaattt agcgatttgt gtagctgctt catagatctg tgcagctagt
    24901 tccctacttg gagctgtaat aacaacttgt acttcctctt tttctgcatt gattttttca
    24961 aacaaaggca acaaaaacgt atgtgttttt cctgtaccag tttgagattg cccaatgata
    25021 ttttttcctt ttgcaattaa aggaatcaaa cgttcctgga cctctgtggg ctgtttgaag
    25081 ccctttgcgg ttaacgcttc attgataaac ggtttgaagc ggaatatatc aaaattattc
    25141 attcgtgcac ctcttctctt taaattatca cgactttggc attataacac agacaaagcg
    25201 tttttgctca gctaatttcg agaatcatca tcctttgttt ggccaatcgt gctaacagta
    25261 tatgtgtgac tagctttttc ataaaacaga atccgttttt ttctcaaaaa tgatagcaag
    25321 atatttaaaa taaaaatacc ttgtagtaac aagacaccga gagaaatgaa tactgcactc
    25381 atctgcaaca ccgtattcgt tgtcgaattc atgatttgct gaagcaactg ccccaaccag
    25441 cttaaaccat aaaaaagaaa gtacagatac ccgtagcgga tcatcaatgc ttttaaagaa
    25501 attcgttcgc ggtcttcttc caccaaacgg atacgcacaa tttttttgcc aagtgtccat
    25561 ccattggtta taaacggtac aatcataaag tataccccta cttctaataa aagccaccag
    25621 atatgagaag taactgcccc tgaatcgaac gcaattttaa agaaaatcgc cgctaccaaa
    25681 ttaataagag ataaaaatgt ccagtcgatc aaataagcga ccaagcgtcg agtcaatgag
    25741 acagtctttc ctttttcata agaaagttca tccattcgtc ttcttgtagg aagcataaat
    25801 gtaaataacg gcgtaacgac aaaaccaatg attccaccca atgtatttgt cactaaatca
    25861 ttcacatcaa ataagcggta tggtcttggg tagataaaat aaagtccact tagctgcgtc
    25921 aattcaaaaa atagagaaag gcagaaacta gcaatcatcg ttttcataaa agaaagccgg
    25981 aaataatatc ttaaataaat gccaaatggt atgagtaata aaacattaaa agctggttcc
    26041 aaaaatacac tctgcttcat tgccggcagg taggtactag gttcagacaa gaccaatacc
    26101 gtgttgtctc tgaaatgttg ccatgaagca aataaatgca gctccatctt tggccctgtc
    26161 atttgggcaa ccgtctcctt tgccggaagt ggtaagatca ccaaaaaata cgcacacaat
    26221 aaataaaaaa cgaaagaata caaaataact gcccttgtta ctagaaatgt tccatacttt
    26281 cgatattcat aaataaaaaa aacagatgag atcgctaagg ctaaaaatgg aaaaacaatc
    26341 agcgcaaaac gaatcggttc gatataactt gccatatgac tcctccttga ttctttgtat
    26401 ctctcttatc ttagcagtcc tttgctgttt caagcaagta acgaggctat tatttctcat
    26461 ttctttcaaa gtaaagtaag ataaacacaa agggggtgtc gagatgctga atatgtactt
    26521 tgtttttggc gtacctattt
//
LOCUS       DACEXQ010000018        25359 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.018, whole genome shotgun sequence.
ACCESSION   DACEXQ010000018 DACEXQ010000000
VERSION     DACEXQ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.018"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..589
                     /locus_tag="IXH04_000568"
     CDS             <1..589
                     /locus_tag="IXH04_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA helicase RecG"
                     /protein_id="HAQ1851201.1"
                     /translation="AVEIYEKLRELFAPKYEVGLLHGKMKNQEKEVIMEAFKDNQMQI
                     LVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRVGRGSDASYCILVANPKNEL
                     GVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLPQFAIADLVTDGNVLEVARE
                     EAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            638..1639
                     /gene="plsX"
                     /locus_tag="IXH04_000569"
     CDS             638..1639
                     /gene="plsX"
                     /locus_tag="IXH04_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAQ1851202.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            1761..2003
                     /gene="acpP"
                     /locus_tag="IXH04_000570"
     CDS             1761..2003
                     /gene="acpP"
                     /locus_tag="IXH04_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ1851203.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            2271..3275
                     /locus_tag="IXH04_000571"
     CDS             2271..3275
                     /locus_tag="IXH04_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1851204.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            3276..4220
                     /locus_tag="IXH04_000572"
     CDS             3276..4220
                     /locus_tag="IXH04_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1851205.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            4220..5182
                     /locus_tag="IXH04_000573"
     CDS             4220..5182
                     /locus_tag="IXH04_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1851206.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            5209..6123
                     /locus_tag="IXH04_000574"
     CDS             5209..6123
                     /locus_tag="IXH04_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1851207.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            6149..7930
                     /locus_tag="IXH04_000575"
     CDS             6149..7930
                     /locus_tag="IXH04_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1851208.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            8278..8964
                     /locus_tag="IXH04_000576"
     CDS             8278..8964
                     /locus_tag="IXH04_000576"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAQ1851209.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            8984..12565
                     /gene="smc"
                     /locus_tag="IXH04_000577"
     CDS             8984..12565
                     /gene="smc"
                     /locus_tag="IXH04_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAQ1851210.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            12574..13383
                     /locus_tag="IXH04_000578"
     CDS             12574..13383
                     /locus_tag="IXH04_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1851211.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            13394..14392
                     /gene="ftsY"
                     /locus_tag="IXH04_000579"
     CDS             13394..14392
                     /gene="ftsY"
                     /locus_tag="IXH04_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAQ1851212.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            14579..16846
                     /gene="gshAB"
                     /locus_tag="IXH04_000580"
                     /pseudo
     CDS             14579..16846
                     /gene="gshAB"
                     /locus_tag="IXH04_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
     gene            complement(16935..17387)
                     /locus_tag="IXH04_000581"
     CDS             complement(16935..17387)
                     /locus_tag="IXH04_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAQ1851213.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            17537..18487
                     /locus_tag="IXH04_000582"
     CDS             17537..18487
                     /locus_tag="IXH04_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ1851214.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            18480..19439
                     /locus_tag="IXH04_000583"
     CDS             18480..19439
                     /locus_tag="IXH04_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ1851215.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            19436..20191
                     /locus_tag="IXH04_000584"
     CDS             19436..20191
                     /locus_tag="IXH04_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1851216.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            20214..21167
                     /locus_tag="IXH04_000585"
     CDS             20214..21167
                     /locus_tag="IXH04_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1851217.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(21811..22107)
                     /locus_tag="IXH04_000586"
     CDS             complement(21811..22107)
                     /locus_tag="IXH04_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAQ1851218.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            22462..22818
                     /locus_tag="IXH04_000587"
     CDS             22462..22818
                     /locus_tag="IXH04_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ1851219.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            22828..23727
                     /locus_tag="IXH04_000588"
     CDS             22828..23727
                     /locus_tag="IXH04_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAQ1851220.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            24244..24975
                     /locus_tag="IXH04_000589"
     CDS             24244..24975
                     /locus_tag="IXH04_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ1851221.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            25120..>25359
                     /locus_tag="IXH04_000590"
     CDS             25120..>25359
                     /locus_tag="IXH04_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HAQ1851222.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGS"
BASE COUNT         8423 a         4435 c         5452 g         7049 t
ORIGIN      
        1 tgcagtggaa atctatgaaa aacttcgtga actatttgca ccaaaatatg aagttggctt
       61 gttacatggt aaaatgaaga atcaggaaaa agaagtaatc atggaagcct tcaaagacaa
      121 ccagatgcag atattagttt cgactactgt aattgaagta ggggtaaacg tgccgaacgc
      181 gaccgtcatg ctgatcatgg atgcggatcg atttggattg gcacagctgc accaacttcg
      241 tggacgtgtt ggacgcggaa gtgatgcttc ctactgtatc ttagttgcta atcctaaaaa
      301 tgaactaggt gtggaacgga tgaaaatcat gacagagacc accaatggtt ttgtactaag
      361 cgaaaaagat ctggaattac gtggtcctgg tgaagtattt ggcttccgtc aatctggtct
      421 gcctcaattt gcgattgctg acttggtgac ggatggaaat gttttggagg tagccagaga
      481 agaggctgca gccctttggc aaataaagga ttggcagatg cttcctgagt atcagccttt
      541 agcgcaaagc ctccaaatag aaaatcagga taatcgttat ttcgattagc agactgtgct
      601 atactttata ctgatgacgg aaaaaggagt ggaaacaatg aaaattgccg tagatgcaat
      661 gggcggagat cacgcacctc aagcaattgt agaaggcgtg gctttagcgc agcaagattt
      721 tccagaagta gaattcttac tttacggaaa agaagcagaa atcaagaaat acttaacaaa
      781 tgaaaaaaac atcacgatta tccatacaga cgaaaaaatc aatagtgatg atgagccagt
      841 aaaagcaatt cgacgaaaaa agactgcatc aatggtcctt gctgcacaag cagtgaaaaa
      901 cggtgaagca gatgctgtat tttcagcagg gaatacaggc gcgctactag cagcagggtt
      961 gttcatcgta ggacgtatca aaaatatcga acgccctggc ttgatgtcta ccttgccagt
     1021 agtagggaaa gaaggtgccg gtttcgatat gctggatctt ggcgctaacg cagaaaacaa
     1081 accagaacat ttgttgcaat acggtatttt aggttctttt tatgcacgta aagtccgagg
     1141 tatcggacgt cctcgtgtcg cactcttaaa caacggaaca gaagcaacaa aaggtagtga
     1201 agtgacgaaa aaagcctatg aactccttca gaatgaaaca agcttgaatt ttattggaaa
     1261 cgtagaagct cgtgagctac tcaatggtgt ggctgatgta gtggtcacag atggatttac
     1321 aggcaacgct gttttgaaat ctattgaagg aacagcaatg aatatgatga atctattaaa
     1381 atctgctatt ctaaatgaag ggatcaaagg aaaaatgggc gctatgcttt taaaagacgg
     1441 cttacgttct ttgaaagcag aaatggatta ttccaaacat ggcggtgccg ttttgtttgg
     1501 cttgaaagca ccagtcatta aaactcatgg agcaacaggc cccgatgctg ttcgctatac
     1561 gatccgccaa atccacacaa tgcttgagac agatgtagtt ggacaattag tcgaacaatt
     1621 tgaaaaaagc gaagaatagt atttggatat ctgaagcgat ccgttccatt gaaaaaaagg
     1681 tggacattcc tgaaaaatgc cagtaaaatt agccatggtg taggtaattg acaagtgtat
     1741 gattcttgga ggtgaaaaag ttgactcgcg aagaagtatt taacaaagta gcgaaaatta
     1801 tttcaaatca ttttgagata gataccgata aagtgacgga tgaattgaac atcaaagatg
     1861 atttaaaagc tgattcaatt agtattatgg aattcgtttt agagcttgaa gatgaattcg
     1921 gtacggaaat ttcagatgaa gacgctgaac aaattgaaac agtcggtggc gcagtagatt
     1981 atatcagcag ccatcttaaa taatttcaaa taaaagcaaa gaaggataaa aagctgcatg
     2041 cagcttttta tccttctttt tttaatcttt ttgacacgta aaagtcattt ttctgataat
     2101 tctggcgata aacagaaatt atgttgcaaa gtaaagcggt tttctatata attattaata
     2161 attcattaca caaagataag aataaccttt gttataaaac gatttattct gtgtaatgtc
     2221 cttttttatt aagaaaaact tccatttagg aagcagagag gggtattcga ttgtccgata
     2281 accaattatt agaagttcag aacctacata caggattccg actcaaagat gactattacg
     2341 atgcagtaga tgacgtttca tttactttag acaaaaacga gattttagca atcgttgggg
     2401 aatctgggtg cgggaaaagt accttggcta ctacgattat gggattactt gatcctaata
     2461 acacaaaaat tacaggtgag attttatata acgatctgaa tttaatcgac ctgaacgaaa
     2521 cgctctataa taaaatccgc ggaaatgata tcggaatgat tttccaagat ccgttgtctg
     2581 cattaaatcc tctcatgaga atcgaagacc agatcaaaga aggactttcc tatcacacaa
     2641 agatgaatgc agaacagcgt caagctcgtg cactagaatt gctagaacaa gtagggattc
     2701 ctaatcctgc gagagttgga cgccaatatc cccatgaact atcaggcggt atgagacaac
     2761 gtgtcatcat cgcaatcgca atcgcctgca aacctccaat cgtcattgca gatgaaccga
     2821 cgacagccct agatgtaacc atccaagcac agattcttga tttactgaaa gaccttcagg
     2881 aagaaacaaa aacagggatc atcttgatca ctcatgactt aggagtcgta gcagaaatgg
     2941 ctgatcgagt ggcagtgatg tatggcggac agttcgttga agtagcaggt gtaaaagaac
     3001 tgtttgatca tcctaagcat ccttatacac gctctttact gaactctata ccacaagaag
     3061 gaaatcatga tgcagaactc catgtgatcg aaggtgtcgt tccttctctg aaaaacatgc
     3121 ctcgtaaagg ttgtcggttt gcaccacgta ttccatggat accagcaagc gatcatgaag
     3181 aagagcctat ccttcatgaa gtcgcaccga accacttggt acgttgctcc tgctataagc
     3241 atttccactt tagagacgaa aaaggagagg tgtagatgac agaattaatc acaatcaaag
     3301 acctgaaagt tcattaccct attcgcagtg gattcttcaa tcgagtaacg gatcatgtat
     3361 atgctgtaga tggtgtggat tttattatcg aaaaaggaaa gacatatggt ttagtgggag
     3421 agtctggctc tggaaaatct actacgggaa aagccgttgt gggattagaa aaggtaacag
     3481 ccggacaaat catgtatgaa ggaaaagatg taacgaagcg aagcaaccgc aaaaaaatgg
     3541 gttataacaa ggatgtgcag atgatcttcc aagattcaat gtccagtttg aatcctaaaa
     3601 agcgagtgtt ggatatcatt gctgaaccca tccgaaattt tgaacggtta agcgatcaag
     3661 aagaaaagaa aaaggtaaaa ggactgctag atatcgttgg gatgccggaa gatgcgctat
     3721 ataaatatcc tcatgaattt tcaggcggac aacgtcaacg tctaggagta gcccgtgctg
     3781 ttgctaccaa tccaaaactg atcgtagccg atgaacctgt atctgctctg gatctatctg
     3841 ttcaagcgca agtattgaat ttcatgaaac gaatccagca agaatacggc ctcagctatt
     3901 tattcatttc acatgattta ggtgtagtaa aacacatgtg cgacaatatc gcaattatgt
     3961 acaaaggtcg atttgtagaa atcggtacaa gagaagacat ttataatgac ccgcgacata
     4021 tctatacgaa acgtctgctt tcagctattc cgcaaatcga tgtagaaaat agggaaagcc
     4081 acaaagaaaa tcgacgaaga gtagaacaag agtatatcca aaaccaaaaa gaatactatg
     4141 atgcgactgg tcgagtctac gatttgcgta cgatcacaac tacacacaaa gtggcgttga
     4201 aagatggagg tgcaagctaa tgtggaaaac gattctgcga cgtgttttat tgatgatccc
     4261 gcaagttatt attttaagtg ttctgatctt cttattagcc caagcgatgc cgggggatcc
     4321 atttaccggt ttgatcaatc caaatcagga tccggcagtg atcgaacaga tgcgacaagc
     4381 agctggcttg aatgacccat ggtatgtcca gtacttccgt tggatcggaa atgctttgca
     4441 aggcgatttt ggtcaaagtt tcttatacaa acgctctgtt gctgacttga tcggcgaacg
     4501 gatcatcaac actttatatc tatcgatctt gacagttgtc cttacttatt tgattgctgt
     4561 gcctctgggg atgcttgctg gccggtatca aaattcaatc ttggataaga ttgtcgtaat
     4621 ctacaacttc tttagttttg ctgttccttt atttatcttc ggcttgatca tgctatttgt
     4681 ttttggttat cgtttaggct ggttcccaac aagtggatca caaacactag gtttgcaagg
     4741 tggatttatc actcaatgga tagaccgttt gaaatatatt ctgttaccat caattaccca
     4801 agcatttttg gcgactgccg tcacaatcca atatctgcga agtgaagtaa tcgattcgaa
     4861 atcgctcgat tttgtcagaa cagcccgttc caaaggggta ccgacgaata aagtattcag
     4921 tcgacatatt ttccgaaatg cagcgttgcc gatggcttct caaatggggt atgaaatcac
     4981 ttcgttgatt gctggttctg tagttatcga aaagattttt ggttatccgg gagtcggaaa
     5041 actatttatt gattccatcg gccaacggga ctataccgtg atcacatcct tagtattgat
     5101 tttaggaatc gcgacgctag tggggacatt actttcagat atcatcatga gtatcgttga
     5161 cccgcgtatt cggattcaat aaaaccaatc agaaaggaga atggatcgat gagtgaaaaa
     5221 aacaatacag cagttcaaga aagtattcct cctatgggga tacgaatgat tgctcgagaa
     5281 tttaaaaaag ataaaattgc tattttttca ttaggtttgc tagtggtttt attattagtg
     5341 atctttatcg gtgcgatgct gaccgatcaa gacaaagtga tgtatgttag tatcttcgac
     5401 aaatacgctg caccaggaac agtttcgaca caaggacaaa agttcattct gggagcagat
     5461 gaaggcggac gtgatgtatt aggacaattg atcataggtg ctagaaactc tgttacgatc
     5521 ggatttgcga tcacgatcct tacttcgatc atcggggttg gattagggat catctctggc
     5581 tactacggag ggatcattga caacatcttg atgcggatcg ttgattttat catgattcta
     5641 ccgatcatgc tgatcattat cgtcttcgta tcgattattt ccagatacaa tgtctggtcc
     5701 tttatattca tcatgagtgc attttattgg gtagctaaag cccgattatt ccgaagcaag
     5761 acattatctg aagcaagaag agattatgtc agtgcctcaa aaacaatggg aaccaatgat
     5821 tttaaaatca tgttccgcga aatcatgccg aatctaagtt cattaatcat tactaatttg
     5881 acgatgaact ttgcggcaaa catcggtatc gagacaactt tgactttctt aggtttcggc
     5941 ttgccggcaa atgtacctag tttaggtaca ttgatcggtt atgcaagtaa cggagaagtc
     6001 ttagtgaatc ggcaatggat ctggttaccc gcatcaatcc ttattttagt tttgatgttg
     6061 agtatcaatt atgttggaca agcattcaag cgttctgcag atgcgcgcca acggttaggc
     6121 tagtatagaa gcgaaggggg aaataggaat gaagaaaacg atttttggat ttgcagcgct
     6181 gatgtctgct gtcgttcttg cagcatgcgg gggcggaggt acagatacct ctggaaacaa
     6241 ctctggaggc aacggcaata acaaagagga attaactttt ccaattgcaa caaaaaataa
     6301 tgatgatgca atcgaagacg gcgaattaga agctgcggtc gcaacggaca gtcagtttaa
     6361 aggattgttc cagtgggagt tttaccaaga ttcatatgat gcagctttta tggctccttc
     6421 tcatgaaccg ctatttgcaa acgatgacga tttcagaatc acagacgacg gagctgcaac
     6481 gcttgatcta gatgaagata cgaaaaaagc gacaatcaaa gtaaaagaca acgtcaaatg
     6541 gtcagatggt gaagatgtaa cggcggacga tgtgattttc ccttacgaaa tcattgggaa
     6601 taaagattac actggtatcc gttatgacga cactttccgt aacatcgtag gtatggaaga
     6661 atatcatgat ggaagtgccg acacaatctc cggaatcaaa aaaatagacg acaaaacagt
     6721 tgaaatcgaa tataaagaag taaacccaag catgcttcaa tcgggtggtg gtatctggtc
     6781 atatgcagca ccaaaacata cgttagaagg tgtagcaatc aaagatatgg aatcaagcga
     6841 tcaagtacgt aaaaatccag tgacttttgg cccttattac atgagtaata tcgttgcagg
     6901 agaatcagta gaatacttgc caaacgagca ctactggaat ggtacgccta aattgaaaaa
     6961 gatcacgatg aaatctgtac cgatggcaag tgcaactgaa gcattgaact ctaaattata
     7021 tgatatgatt ttccaaatgc caacagatac ttacgatacc tataaagaaa ttgctggcta
     7081 tacaaactta ggccgtgaac aaacttctta tacttatcta ggattcaagt taggtaaatg
     7141 gaatgcagat acaaagagtg tggaatacaa tccagattca aaaatggctg ataaatcatt
     7201 acgtcaagca atgggctatg cattagataa taatgcagta ggtgaacgtt tctatgcagg
     7261 tttgagaagc aatgcgactt cattgattcc tccagtattc cagtcatacc atgatgatga
     7321 gatgaaagga ttcacacaag atctagacaa agcgaaaaaa ctattagatg atgctggcta
     7381 caaagatgtg gatgacgatg gtttaagaga agacaaagat ggaaataaac taacaatcaa
     7441 ctttgcttcg atgtccggtg gggaaacagc gcaaccattg gcagattact atatccaaca
     7501 atggaaagaa attggtttgg atgtccaata cacaacaggt cgtctgatcg aattcaattc
     7561 tttctatgat aaattagaaa acgatgatcc tgaaattgat gtttaccaag ctgcttggaa
     7621 tacaggaact gatccaaatc caagtggact atggggacca aatgctgcgt tcaactacac
     7681 tcgttatgaa tcagaagaaa atacaaaatt gatcaacgat atcagttcaa gcaaagcatt
     7741 tgatgaagag taccaaaaag aagcatatca aaaatggcaa caatatgcat ttgaagaagc
     7801 atttgcgatt ccaacgttgt tccgtaatga agtattgcca gtcaacgacc gtgtcaaaga
     7861 ctgggattgg tcttatgatg taccaaaccc atgggcaaat gtttctgtta cagcagattc
     7921 acgttcataa tcactaaaaa tgaccgaaaa agggcgatgt cctttttcgg ttttttgata
     7981 tttttctgtt tatttctcta atattcaggt caaggcgctg atttcatgac ttctccaata
     8041 aacggtgttc aaagtccagt ttctcggtat taagtcaaat ttatgcaaaa atatggaaag
     8101 ccattttcac aaaatttttt cttcatactc gaaactaaac gaatttttca caacttctcg
     8161 tcaatcacta ggattattaa agctttactt accttttgac cgaaccattt caaatttgac
     8221 aaaaaaccag tataataagg aagtatcgaa aaaatatcca taaaaggaat gtggaaaatg
     8281 gacaaccagc tgacaaaaga actaaaagaa aactataaca tcgtttttca cgatctaaat
     8341 ttgcttgaac aagcttttac ccattcatcc tatgtgaatg agcatcgcaa tctgcaatta
     8401 tcggataatg agcgacttga atttttagga gatgccgttt tagaactgat ggtttctcag
     8461 tatttattcc gtttatatcc tcatttgcca gaaggaaaat tgacaaaaac acgagctgcg
     8521 atcgttcgcg aagacagctt gtcgaaattt gccaaagaat gtcactttga tcaatatgtt
     8581 ttgttgggca agggagaaga gaattcagga ggacgtacgc gaccagcatt gctttgcgac
     8641 ctattcgaag cttttttagg cgcgttatat cttgaccagg gattcgacac gacccatgca
     8701 ttcatcgaaa aagtgatttt tcctaaagtc aaagcaggtg ctttttcaca tgagatggat
     8761 cataaaacaa aactacaaga agtcttgcaa aaatcaggtg atgtatcaat cgaataccga
     8821 ttgatcaatg aagaaggtcc agctcatgaa cgagtatttt ggatcgaagt ttatgtagat
     8881 gatcaattga ttggcactgg acaagggaaa tcgaaaaaat tagcagaaca agctgcggcg
     8941 gaaaatgcat tggcagcact gtaaaagtaa gtgagggaaa gctttgtatt tgaaacgaat
     9001 tgaaatagcc ggttttaaat catttgcaga caagacagtc attgattttg aaaatagcgt
     9061 cacagcagtg gttggaccaa atggcagcgg aaaaagcaat attacagagg ctatccgctg
     9121 ggtattagga gaacaatctg ctaaaagtct gcgaggcgga aaaatgccgg atatcatctt
     9181 tgccggttcc gatacccgta agcagctgaa tattgcagaa gtcacagtga ttttagacaa
     9241 tagtgatcat tatctgccat tggagtacaa tgagatcagt gtaacccgcc gttatcgacg
     9301 gactggggaa agcgagtttt tcatcaataa acaaagctgc agattaaaag atatccaaga
     9361 attgttctta gattctggat taggaaaaga atcgttctca atcatttctc aaggaaaagt
     9421 agaagcgatt ttcagtagta aaccggaaga ccgtcgtggg atctttgaag aagcagctgg
     9481 tgtcttgaaa tacaagcagc ggaaaaagaa agcagaacag aagctttttg aaacagaaga
     9541 caacctcagc cgcgtccaag acatcatcca cgaattggaa gaacagttaa ctcctttggc
     9601 cgcccagagt gaggcagcaa aggagttttt acggctaaaa gaaacactga ctcaaaccga
     9661 tgtctctttg atggttgctg aaatcaagac agcaaaaaaa gattgggaca acaaacaagc
     9721 acagcttgcg aaattcaatc tagaattagg caaactatct gagtcgatcc aagaacaaga
     9781 atccattttg gcgaagcagc gtaaagaaaa tgcgcaagcc gatcgtctga tcgaaaaaaa
     9841 tcagcaagta ttattagatc tttcggaaaa actgaagcag acagaaggac aaaaagatgt
     9901 tttgcaggag cggacaaagc atacacaaaa aagctcgcaa gaatatcaaa cttcattagc
     9961 agaagcacaa aaaaaggtta aacattttga aaagcttcaa gaaagcttga tgaaagcagc
    10021 agcagaaaaa gaaacagaga tccaaaaagc tgaagcaaat ctcataaaaa cacagcaaga
    10081 attagaaaaa taccaaaaat caacgaaaga attattggca gaattaagag atcaatatgt
    10141 tgatttgatg caagaacaag cagctgtagg aaacgaactg aagtatctag aacgtcaata
    10201 tatacaagaa acagcaaaaa gcaagcagac gttagccaag caatcggaag tagaagcatc
    10261 agtagaccga ttgatcttac aaaaagaaga attgacacaa aagcaggcgc agctaaaatc
    10321 atctttaact gaaacaaaag agaagttaga aatgatccaa caaaacggga aaaagttcca
    10381 agaagcatta gcaaaagaac agcctaaaat gtatcaatta atgaatcaag tccagcagct
    10441 acgtgctcgt aagaaaagcc ttcaagaaat ccaagaaaac tattttggtt tttaccaagg
    10501 ggttcgattg gtcttgcagc ataaacagca gctttcgggt attgtcggag cagtggctga
    10561 actgatcgat gttcctgcag atttcacgtt agctatcgaa acagctttag gtggggcagc
    10621 ccagcatgtc atcgttgaaa atgaaaaaga tgctcggcaa gccatcacgt atttgaaaca
    10681 gcaaagagga ggaagagcaa cattccttcc actgacgaca atcaaaccaa gacaattacc
    10741 ggctcatata ttgacacaag cagcagctgt agaagggttt atagggattg ccagtgaaca
    10801 agtcagttat cccgatcaaa tccaaactgt ggttcaaaac ctgctaggaa cgatcctttt
    10861 agccaaagac ttaactagtg ccaatgcaat cgcgcaaacg atccgctatc aatatcgagt
    10921 ggtttcctta gaaggtgatg tgatgaatgc cggaggatcc atgacaggtg gagcgaataa
    10981 acgaggaaac caaggcagtt tgtttgtaca aaatcaagaa ttgaaacagt tgactagtga
    11041 atttgaagaa gcagataaac aactccaagc acaagaaaaa aaagttcagg agctgcaaca
    11101 agaaacagct cgtttagccg aagaacaaga agttttgcgt acacgaggcg aacaattacg
    11161 ttttgaagaa caagaagcga caaatcagct acaaaacatc ataaatgaac ttgaacgttt
    11221 tgaaaaagaa aagcaaatct caacttttga gacacgtgaa ctgcagcagt ttatcgaaga
    11281 ttacgaaaag caaacgaacg aattgaaaga caaacaaaca gatcttgaat cgcaacgaca
    11341 aaaaatcgac gaagaaatca aatcgttaag tcaagaaagc gaccagatgg aagccagaag
    11401 agcacaagta caaagtcaaa aagcacaaga acaagcagat cttgctgtct tgaaagagca
    11461 gtttaatcat ctgcagatcc agctccgcgg tgcccgagtc caaaaagcag aggcaaccga
    11521 acgtcaagaa gcgatagaaa agcagttggc gacattgaca gcagactttt ctgatcatga
    11581 agtcacagaa gaaagtttgg aaaaacaaat taacgaatta tctgctcagc gagaaacatt
    11641 aaaagccgag ttggcaaaag ccaaagagca gcgagataga aggcaaaagg agatcgataa
    11701 gcttgaagca gtcttggcag agcgtaatcg tgagcaaaaa gcacgactgt cggaacaatc
    11761 caaattagaa gtccaaaaag accgagctga aatgatttta gataatcatt tgacctatct
    11821 gcagtcagaa tatcagctga catttgagaa agcttcccaa gactatcaag aaacaaccga
    11881 tatcgaagac agtagaacaa aggtttcatc cttaaaagaa cagattgaaa aactgggacc
    11941 agtaaactta aattcaatcg aacaatacga acaagtcagt gaaagacata cattcttagc
    12001 aacacagcga gatgatctgt tagcagcgaa aaaccaattg tttgaaacga tggatgagat
    12061 ggatgatgaa gtgcggacaa gattcaaaga agtatttgaa gctatccgtc aagaattcaa
    12121 aattgtcttt ccaaatatgt ttggcggcgg taaagctgaa ttagtactga cggaaccaac
    12181 tgatttgctg aaaacaggta ttgagattga agtacaacct cctggcaaaa aactgcaaag
    12241 tctaagtttg ctttctggag gagaacgagc acttacagcc atcgcgttgc tcttttccat
    12301 tatccgtgtc tgtcctgttc cgttttgtat cttagatgag gtagaagcag cgttagatga
    12361 agccaacgtg aaacgttttg gtcgttactt gagcgatttc caagatgaca cacaatttat
    12421 cgtagtcaca caccgcaaag gaacgatggt cgcagcagat gtactgtacg gagtgacgat
    12481 gcaggaatcc ggtgtatcaa aaatcgtttc tgtacggatg gaagatatca atgaagaggc
    12541 gaaaatcaac gaaacgaaag tgtaggaacg aaaatgatca aattagtagc aatcgatttg
    12601 gacggtacat tattaaatga acaaaagctg atttcagatg agaataaaca agcattagca
    12661 caagcaaaac agcaaggggt caagatcgtt ctatgtacag gacgcccctt agctgcgatg
    12721 gcgcattatc tgcaagaatt aggactggta gatgaaggtg actacagcat cactttcaac
    12781 ggcggtttgg tacaaaaaaa tgatactggt gaaatcatgg aaaagaaagt gatggaagta
    12841 tcagatatcc aacgcttata caaactggct cagcaattgg atctgccact agatgttttg
    12901 tctgacaatg tggttttaca gctgcctagt gccccaagga agcaatcctt gtacaatgtg
    12961 ttgaataagc tgctgcaatt ccaaccagcg gatataagcg atattacaga tgaatttgtg
    13021 cttaataagg cagtaatcgc ttatgaccaa gaagaattag atcctaaaat caaagaaata
    13081 ccagcagaat tccatgaaat gtatgagatc atcaaaacaa gaagtatgct tttggaattt
    13141 atgccaaaag gtgttacaaa agcttatggt atttctttat tggcaaaaga tcttggtttg
    13201 aaacaagaag aaatcatggc gatcggtgat gaggaaaatg atttaccgat gatccaatat
    13261 gctggactag gcgtagcgat ggagaatgct gtgcctttcg tcaaagaagc agcagatttc
    13321 gtgacagcca gcaatgaaga aaatggcgta gccaaagtga tccaaaaatt tgtattagag
    13381 tagggggagt caaatgggat tttttgataa aatcaagcgt gcttttactg gcgaagagaa
    13441 aacagaagaa gttgaagtac aggagaagta cgacaagggt ttagaaaaga cgcgtaaaac
    13501 atttggtgaa cggatgaatg agttgtttgc caattttcga acagtcgatg aagacttttt
    13561 tgaagaatta gaagaaacac ttatcggtgc agatgtaggt tttgaaacag cgatacgaat
    13621 taccgaagca ttacgtcaag aagtgaaact cagaaatgcg aaaaaatcgg cagaagtcca
    13681 aaatgcgatc atcgagaaac ttgtggatct ctatgaagcg gaaggaatca atgaagtaaa
    13741 tgaactaaat atccaagaag acggactaac agttattttg tttgtcggtg ttaatggtgt
    13801 agggaaaaca acaagtattg gtaaacttgc ccatcagttc aaaaacgaag gtaaaaaagt
    13861 attgatggca gcagcagata ccttccgcgc tggagcaatc gaccagttag ttgtctgggg
    13921 cgaacgcgct ggtgttgaag ttgttcgtgg caatgcagga ggcgatccag cagctgtcgt
    13981 attcgatgct ttggaaagag caaagacaga gcaggcagat gttttactag ttgatactgc
    14041 aggccgactg caaaataaag tgaacttgat gaacgaatta gaaaaaataa aacgagtaat
    14101 ccagcgggaa ttaccagaag caccccaaga aacacttctt gtattagatg caaccactgg
    14161 tcaaaacgcg atggttcaag caaaacaatt caaagaaaca acagatgtga ccggtcttgt
    14221 tttgacaaaa ttagatggta cagcaaaggg cggtatcgta cttgctatcc gtaatgaatt
    14281 gcatttacca gtgaaattgg tcggtcttgg tgaaggaatc gatgacttgg agccgtttga
    14341 tccaaatgat tttgtcgttg gattgttcaa aggattacta aaagaagaat aacccatgtc
    14401 taaaaagcag gagctgtgac aaaagttgca gctccttgtt tgaatgattg tttttaacat
    14461 agctgtgttt ttgatgcctt catttttata cttatttgta agcggttctc aaacaatttc
    14521 gttctttttc ttagactttt tgattataat tagattcata tgaattggag gtcacttaat
    14581 gatgaatttt aagcaattat tattgcatgt caatgcgcgc ccttttatcg accaagctcg
    14641 atttggtata gaacgggaag gacagcgggt tgatcttgca ggaaatctag caaaaaccga
    14701 tcatccagca atctttggcg atcgatccta tcacccctat atccaaacag attttagcga
    14761 aacacaaaca gagatgatca cccctgttac cgattctatt cccgaattat ttcagtatct
    14821 cgctgctgtt tatgatgtga ctgctcgttc tataccgaaa gaagagatga tctggccatt
    14881 aagtatgcca cctgccttac cggaaaaaga cgaagagatc attattgcaa aattaaaaaa
    14941 tttcgaagat gtcttgtatc gacgttattt agcaaaagaa tacgggaagc gaaaacagat
    15001 ggtaagcggt attcatttca attttgaatt tggcgatgag ttgctaagaa cattgttcag
    15061 ccatcaggaa gaatttcaag atttttccga attcaaaaca gaactttatt tgaaaacagc
    15121 gagaaacttt atgcgctatc gctggatgat cacttattta ttcggtgctt caccaatgag
    15181 tgagaagaac tactttttag atgaatcaca tccgcaagaa cctgttcgca gtattcgcaa
    15241 cagtgcgctg ggctatacga atcatccaaa tgtgaaagtt tcctatgctt ctatgaaaca
    15301 gtatttagca gatatcgagc gaatgatcga agaagggaaa ctttcggagg agaaagaatt
    15361 ttatacaccg cttcgtttcc gaggcggaaa gaaagtcgca gatttagcaa caacaggtgt
    15421 tcgttatatt gaattgcgga atatcgattt gaatccttat gcaagattgg gtatcaatcc
    15481 agagcaagtt cggttcttac aattattcct gatgtacatg ttatggacag aagaaaaaga
    15541 agactgtgac caatgggtag cagaaggtac gactcgaaat aacaaagtag cactagaaca
    15601 accatcagat caaacagaat ttcatcaaga aggtagagaa attcttgaag gaatgaagca
    15661 aatgctggtt gaattggatt ggttggattc tctttattta gtagaggaag cattgactca
    15721 aatggatcat cctgaacaaa cgttagcagc gaaactctat caagaagcac agctatcgag
    15781 tcagcaagaa gttgctgtgg cattgggaca tcaatattat aaagaaagcc atgaacgtcc
    15841 ctatcagttg gccggttttc gcgaaatgga actttctact cagatattta tgttcgatgc
    15901 catccaaaaa ggtgtccaag tcaaagtatt agatgagtcg gatcaatttt tgcgtctgca
    15961 attccaagac catgtagaat atgtgaaaaa tgcaaacatg acaagtaagg acagctatat
    16021 cgtcccactt atcatggaaa acaaaacagt aacgaaaaaa gtcttaaaag aagctgggtt
    16081 ccgggtacca ggaggcgcag aattttcatc catggaagaa gcggtaaaag cttatccgag
    16141 atttgccgag caggcatttg tgatcaaacc taaatcaacc aattacgggt taggtatcac
    16201 gattttcaag gaaggagcta gtctggaaga ttatcaagca ggtttagcca ttgcttttcg
    16261 tgaagacagt tcggttttag tagaagaatt tatgccggga acggaatatc gtttctttgt
    16321 gatcgacgga gaagtccaag ccatcatgtt gcgagtccct gctaatgtta ttggtgacag
    16381 tatccgaaca gtgaaagaac ttgttgaaga aaaaaatagt gaccctttgc gtggaacgaa
    16441 tcatcgtgca cctttagaat tgatccaatt aggtgaatta gaacaattga tgttgaaaga
    16501 acaagggttg acgatcgaat cagttcccca agccaatcaa attgtttatc taagggagaa
    16561 ctcaaatatc agtacaggtg gtgattcgat cgatatgact gatgaatttt ctgaagctta
    16621 caaaaaaatc gccgtttctg cggtagaagc actaggagct aagattagtg gtatcgattt
    16681 gattatacca gataaataaa tcgatcctac aactgataaa aaagcatatg ggatcatcga
    16741 agcaaatttc aatccagcca tgcatatgca tgtttatcct tttgcaggaa aaggcagaag
    16801 attgacgatg aatgtattga aattgttata tcctgaagta ttttaaaaga aaaagctttt
    16861 gacagattgt cctatttgat gtcaatctga tcaaaagctt tttttgcatg taaaaattta
    16921 tgtgtttttt cgttttaaaa taaacgatcc cagaagcttt ttttcttttt tggtttttct
    16981 ttttcagctg gcgaagagtt ttcttctggg tatttttttt cgatatcaat cacaggttca
    17041 tttactgctt cttttgctgt taagagcaat ccgaattcct caggatttgc agtgacgtaa
    17101 tcgttgacta cagtgaaacg gctattgttt ttcgttgcta gctggatata ttggttttgg
    17161 acagaaaggg gtagagcccc attcaagagt atggaagcat ctggatgttt tttgagttct
    17221 tttaaaaagt tttctttgtt ttttggttgg atcatttcag caacagtcat actaagatag
    17281 cagcgttccc tgaatgtccc taaatattta tgctgttctt cagggttcac taaaggtgta
    17341 ccataaagtc ccttatccaa atggcttttt acatcatctt gtgccataaa aaataccctc
    17401 cttcttctct ttgtctttct tacgattata gcatattccc aataatacct aaaacatatg
    17461 aaaaagcggt tgcttttgtt caacgattca cttaaaataa gaatgaaaat cattctcaat
    17521 tagaaaggtg gattggatgc gctacggacg actagtagca ttattagtta ttttaagtag
    17581 tatttcattg ttcatcggag tatcgaatgt acaattgtca gaactgctcc agttagatgg
    17641 gcaaagctta caaatcttgc tagtgactcg tttgccacga acgatttgtt tgatccttgt
    17701 gggagcgacc agtagtattt gtgggttgat catgcaacat ttgacccaaa ataaatttgt
    17761 ttcaccaacg actgcgggca cgatggatag tgcgcgttta ggtattttag tggctatgat
    17821 tttcctacct ggtgcatcct tgattgtccg atccttgaca gcattttgct ttgcttttgc
    17881 aggaacattg ctttttttat cccttacgcg attgttcctc cagaaaaatc aattgatgct
    17941 tccgttgatt ggagtcatgt tagggaatat cattggttca gttgctactt tttttgctta
    18001 tcaattccag ttggtacaaa atatgacttc atggcttcaa gggaattttg cgactgtgat
    18061 acgaggaaat tatgagttat tgtatgctgt tgttccattg ttgctcttgg tttggctatt
    18121 tgcttaccga tttacgatca ttggtctggg cgaatcgttt gcagtaaatc tgggaataga
    18181 ttaccagaag atgcagtttt taggcattgg cttagtatca ttggctagtg cagtcatgtt
    18241 gatcatggta ggaagcattc cttttatcgg agtgatcatt ccgaatcttg tctctagaat
    18301 atatggagat caagtgcaca aaacaattgg cgtaactgct ttagcaggta gtttgttctt
    18361 agtattttgc gatcttgtcg ctcgcgtgtt gatttttcct tatgaaatac cagtcagtgt
    18421 gatcgttgga atcatcggtg gggctatctt cctttatctt ctgataaggg gacgacgata
    18481 tgcttaaaaa aaagccatct atttttattg gtatgcttgt tcttgtttta tttgttacag
    18541 gaatgtactt gacgatccat acatatggaa actggcattt tgcatgtgca ttgaggagta
    18601 agaaaatttt tgcatttatc cttgtctcgc tagctgtcag cagtgccaca atcagctttc
    18661 agacaattac gcataaccag tttttgactc cttcagtatt gggacttgag tcgttatatg
    18721 tccttgtcca aacggtcctt ttcttcttta tcggaggagt gacgatgctt cagcagacag
    18781 gtacagtttt tttcttattg aacgttgggc tgatggtcgg gctgagtcta ttgttatttt
    18841 tctttctttt gaaaaaaatg agagcgaatc tttttttatt actgatgtca gggatgattt
    18901 taggtacgct ttttggtagt gtcagtacct ttttacaagt catcatggat ccgaatgaat
    18961 atgatttgct tcaaggcaga ttatttgcaa gctttggcaa tatccagagt cagtatcttt
    19021 tgcctgcttc tttgatcatt atcggctgct ggtacctgtt atttcgatat cgacatgtgc
    19081 tagacgtttt ccatctcgga aatgaacaag cagtaaacct cgggatagac acagatcgtt
    19141 ttgaattttt gattttatgt tgtgtcagtc ttttgacagg gacatcaacg gctcttgtgg
    19201 gtccagttac ttttttaggg tttattgtcg caaatatcag ttatcgtgtc ttttctactt
    19261 atcgtcatgg tcagctattt atcggaagct ttctggtagc tatgctgttt ctgacaggag
    19321 gacagctcgc agtagagcag attttcggct ggaatacaac agtgagtgtc gtcgttgaat
    19381 ttgtcggagg aatttatttt gtcacaaaat tgattcgcga aaggaagagt atcctatgat
    19441 tgatttacaa aaggtcaata aactgtacca tggaaaatca gtggttaaag atgtacagtt
    19501 aacgattcct gaaaagaaat tgactgcttt tatcggtcca aatggtgctg gaaagtcaac
    19561 ggtgttgtcg atgatcagtc gtttgatacc aaaagataca ggtgaaatct atttagacca
    19621 taacgaagta aaggcttgga aatccaatga attagccaaa aaattatcga tcctacgaca
    19681 atcgaatgaa ataaatgtcc aaatcaccgt gaaagaatta gtagaatttg gacgatttcc
    19741 ttatacgaaa ggaaaattga cgacaaaaga cgaggaaatc gttcaggaat cactggaaca
    19801 ccttgggtta gaagaaatag cggatcaatc gattgacact ctttcaggcg gtcaactcca
    19861 acgtgcgtat attgcaatgg tgctagcgca agacacggat tatattttac tggatgaacc
    19921 tttgaacaat ctagatatga attttgcggt tcaaatcatg cagattttaa aaaatttagt
    19981 acaagaaatc gggaaaacaa ttgttattgt tcttcatgat atcaactttg ctgcaagtta
    20041 tgcagacgaa attgttgcaa tgaaagatgg acagttattt acgcaaggaa cgacccaaga
    20101 aatgataaaa aaagatgtat tggatgaatt atacgaaatg gacatacgga tttgtgagat
    20161 tgaaggccgc agattctgtc tttatttcta aaactaacat taggagagaa gagatgaaaa
    20221 aattagtagt aggtatcttg attctagcat catttggttt agcagcatgt ggaaatagca
    20281 acaacaccag tcaggcagac acaaaaaaaa gcagcacaca aactgaaact acattgacga
    20341 taacagatag taatggagac caaatcgagg tgccaaataa tccagaaaaa gtcgtcgtat
    20401 tcgacaatgg ttctttagat acgatggatg ctcttggtgt tggagataag gtagttggag
    20461 cagctacaag cagccttcct gagtatcttt catcctataa aaaagtagaa tcagcaggtg
    20521 gaataaaaga accagactta gaaaaaatta atcagcttca gccagatcta atcatcattt
    20581 ctggccgaca aagagatttt caaaaagatc taagtcaaat tgctccgacc attttccttt
    20641 ctttagatgc gaaaaatcca tgggaatctt tccagcaaaa tgtcactgct ttaggggaga
    20701 tctttggtaa gcaagaagaa gctaaaactc aattagagga attatctagt gcaatcgatc
    20761 agacgaaaaa gaaagcagaa gctactgaca aaaaagcact agtcacactt gtgaatgaag
    20821 ggcaattatc agcctacgga tctggttcta gattcggttt catccatgat ttatttggct
    20881 ttgaacaagc agatgatcaa atcgaagcat ccactcacgg tcaaagtgtt tcttatgaat
    20941 atgtgctgga aaaaaatccg gatatcttat ttgtagtaga cagaacaaaa gctattggtg
    21001 gagacgacag caaagatgat atttctgcca atgaactggt agcacaaacg aatgctggta
    21061 aaaatcagca gatcatctcc cttgaaccag atgtctggta tttaagcggg ggcggtttag
    21121 aatcaatgaa actgatgatc gaagatgtga atcaagcatt taaatagcat aaataacaga
    21181 ggattaaact ctcatactat gtaacaataa tgtttcttac attcttaaat agcaagagcc
    21241 tatcctcaaa gaagttatca aaaaccatcc gtaaaatcgt attgatgatt ttacggatgg
    21301 tttttattta tttgagaaat cttttttcaa cgtgaattca ttatctacat catacgggtt
    21361 gattctagaa tggggagtgg caaaaaatag tgtgatccat tttttcagca tcaagatcgc
    21421 ctcctttgat ttgaatcaaa ggtacaaagc gaaacttaga taaaacttaa atttagatat
    21481 ttttattttg taccgatctc ttctttaaac gatgttccat ctgctgcttc tcggcaataa
    21541 gtccactaga ctagaaaata tgagaagcta ttttctgtcc agcgttctta ttactcaaag
    21601 caaatcgcag ttgtcacaat ctcttctaaa cgatgttcca tctgctgctt ctcggcaata
    21661 agcccactag actagaaaat atgagaagct attttctgtc cagcgttctt attactcaaa
    21721 gcaaatcgca gttgtcacaa tctcttctaa acaatgttca atcagctgac cttcggtatt
    21781 agctcaaagc actgatttca caacctctat ttaggcatgt gtctcagcaa gcggttgata
    21841 gttgatgatc tgcatttttc gataaaaatc ggcgggaagc ttactccacc gttttaataa
    21901 ttgatagaaa taactctctt ctgctttgat tttatactcg cttaatatca atccaaaact
    21961 attcgttttt tttgaagcta attttttata aatatccaat gctttggttt tatcttcatt
    22021 gacaaagagt gtttttactt caatataaga accctcgata ttttgtgttt cttcgataaa
    22081 atagatgttt tttatatatt ccttcatagt gattcctcca aagaagtctt ttatttgata
    22141 agtttactct accgaagagt tgttaataat ccgtgaacaa aaagtaattc tttttccaat
    22201 agttgttaaa ggttttttat tttttatagt attttttgtt atttaagaaa ggaataaggt
    22261 taaacaaata acaggttgct ttttgggtct ttttccgttc aagaaactga acgaagaaaa
    22321 gggaaagttg tctcttttgt gataagagaa atcataataa acgaatgact tactttccaa
    22381 taaattgaga acagtcagaa ccagacaaat tagttgcatc tgatgaataa agtgattatt
    22441 ctatgacatg aggtgataca gatgaaatta gaagtgacaa aagaagcaca agaagtattg
    22501 aagaataaat tagaagcaga cgatcaactt ttattagata tcgaaaacgg tgacggtcca
    22561 tttgcagaaa gccaagtcag ttgccaattg gatacggcat ttcgattgat cattgtcaaa
    22621 aaagatacac aaacagatct aagtctttat tctgtagtgg ctgatacacc agttggacca
    22681 atcaaaatca aagacagtgc aatactttac atggatgatc catccacact tgctttagaa
    22741 ccaacttata atagtttaca attaaaagga ccaagcggct tgagaaaagg caacttgcaa
    22801 gttgttcgaa aagactagag ggataaaatg ggaaaattgg cgatcatcag cgacctacat
    22861 gtggacatca ataaattcgg cgaatcagag ctgatgcagc tttggcaagt tttgcaaaag
    22921 cacaagatca ctcggcttca tctagccgga gatacggcta ataaagtaga ccgttgccta
    22981 gctgttgtcg aatttttcgt caataaagga ttatctacga catttcattt cggcaatcac
    23041 gagttggcag atgtgcccaa tgaacagatg atggaccatt ttccagaccc acatttttta
    23101 aatgaacggt atattgcttt aaatgagcag accgttcttt tagggatgaa tggctggtac
    23161 gactatcagt tttccgaatt gaaagattac gaggcaatcg tacgaatgaa gcgtttgtat
    23221 tggtatgatc ggttcatcca gcgtgggaaa acagatctcg aggtcaatgc agaaatgctt
    23281 gcagctgcaa aaaatttgct agaccagctg gaagcaaaaa aacttgaggt gattttatcc
    23341 actcatttcg ttcctaaacg ggcatttatc atgtatcaat ctgctcctta tgagcgttgg
    23401 aacaaattaa atgcattttt aggatcagaa tcattcggag aattacttga ccgttattca
    23461 aatatcaaac aagtagtttt tggccatacg catcgtcgct ttgaagataa atccatcggt
    23521 cacacgattt acagctgcag accttttggt tattattatg aatggcagct gacccgagat
    23581 tttgtcttga aagaacagtt gctagacagc tatgatccta tgaaattacg tgtcctcttg
    23641 cgtgcccatc acccagcttt tcaggcctat caaacacaac atttgcaaga ggaatttgaa
    23701 caagcgatga caatcgttcc gtattgagac agcattagta atgtgtaaaa gaaaatacct
    23761 tgtaaaacat aaggataagt aagagcagta gatccattta tcgtggatat gctgcttttt
    23821 ttgcggaaaa taatcagcga atgtttattt gatcggatga tttgtaactc gcagagtact
    23881 agatgcaagc gtgtattctt tttttgaacg ataatcacaa aaaaaaatat aatatacatt
    23941 ttttatttaa ttataattaa tattgattga agtctaggca gtcaagtcac attttgatag
    24001 ataggagggg gacttacgaa ttattttaaa ggctatgaca gctgtatttc tagattgtgt
    24061 ttgcatcaag ttgcaataaa aactagaaat caaatcgggc tgataatcat ttataactaa
    24121 tcgatacata ggaccaaata taggtctatg ttcttttatt tcataagtat cgtaaaacag
    24181 aattttgcta tggattaatc caatcgactg atatgtttag taaataaaga aagaggaatg
    24241 tcaatgatga aaaaagaaat cgtattaaca gcagcaatga tgttattcag catggtagca
    24301 agtacgactt ttgtttctgc aactgaagta tacccaaagg aatataacac agaaggaacg
    24361 attacatttg aagcaggaga tgagggtgta actcctcctg ttgatccaga gaatcctgat
    24421 cccaataaac ctgtcgatcc atcagatcct ccaagccctg gaacaggagg ggcattatcg
    24481 attgattatg gatcaaaatt caaatttggg actcaaaaaa tttctactgc ggataaaacg
    24541 tattatgcag ctgctgatgt gatgaacgat gggtctagaa aaccgacata tgtccaagtg
    24601 acagatagga gaagtacctt atctgggtgg aaattaagtg tctctcaacc agaacagttt
    24661 aaaactgcca gtggggatga actagttgga gcacagctga aatttacgaa agggcaggct
    24721 gtttctttgg ttgatccaac atatacgccg caaacggtga attctgaatt aacgttgaca
    24781 ccgggaggaa acaatacgtt agcaattaat gctaaatcag gaacaggtgt ggggacttgg
    24841 gtctatcgat ttggtgcaaa tgaaaatgaa aatcaagatg cggtacagct ttcggtaccc
    24901 ggtaaatctg tcaagttagc gcaacaatat tccactaaat tagtttggac attagaagat
    24961 acacctaaca actagtttac tgaaaagaac tgttaaaaat aaatacgtag tcatattgtc
    25021 ttgcgaggta atcgagcaaa aattgtcaaa ggctaggcta aatttgtttg gatacgaata
    25081 gttcatctag gaatttaacg atcaggagga aaagatatat tgaaaaagag gaggatcgtg
    25141 ctcattttaa gcagtctctt agtgactaat atctttccga tcatagggta ctctttggaa
    25201 ttgaatcagt tggagcatgg aaatagaaga gacgaagaaa gcaaactcac ttcgacattc
    25261 gacagtttaa tagaatccaa ccagacacaa gaaagcagtg aggatttaag tatttcgagt
    25321 agtggagaga ccactgacaa cgtagaagct gagggttcg
//
LOCUS       DACEXQ010000019        25060 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.019, whole genome shotgun sequence.
ACCESSION   DACEXQ010000019 DACEXQ010000000
VERSION     DACEXQ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25060
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.019"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(105..629)
                     /locus_tag="IXH04_000591"
     CDS             complement(105..629)
                     /locus_tag="IXH04_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1851223.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="IXH04_000592"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="IXH04_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAQ1851224.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="IXH04_000593"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="IXH04_000593"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAQ1851225.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="IXH04_000594"
     CDS             complement(2351..3031)
                     /locus_tag="IXH04_000594"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ1851226.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="IXH04_000595"
     CDS             3157..4026
                     /locus_tag="IXH04_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1851227.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="IXH04_000596"
     CDS             4052..4567
                     /locus_tag="IXH04_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851228.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5177)
                     /locus_tag="IXH04_000597"
     CDS             complement(4614..5177)
                     /locus_tag="IXH04_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1851229.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="IXH04_000598"
     CDS             complement(5232..5411)
                     /locus_tag="IXH04_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851230.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="IXH04_000599"
     CDS             complement(5488..5997)
                     /locus_tag="IXH04_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851231.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="IXH04_000600"
     CDS             complement(6093..6473)
                     /locus_tag="IXH04_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ1851232.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6803..7552
                     /locus_tag="IXH04_000601"
     CDS             6803..7552
                     /locus_tag="IXH04_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAQ1851233.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7736..9013)
                     /locus_tag="IXH04_000602"
     CDS             complement(7736..9013)
                     /locus_tag="IXH04_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851234.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="IXH04_000603"
     CDS             complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="IXH04_000603"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAQ1851235.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(9935..10879)
                     /locus_tag="IXH04_000604"
     CDS             complement(9935..10879)
                     /locus_tag="IXH04_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAQ1851236.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(10881..11567)
                     /locus_tag="IXH04_000605"
     CDS             complement(10881..11567)
                     /locus_tag="IXH04_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAQ1851237.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            12041..12361
                     /locus_tag="IXH04_000606"
     CDS             12041..12361
                     /locus_tag="IXH04_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAQ1851238.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            12515..13420
                     /locus_tag="IXH04_000607"
     CDS             12515..13420
                     /locus_tag="IXH04_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1851239.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            13417..14190
                     /locus_tag="IXH04_000608"
     CDS             13417..14190
                     /locus_tag="IXH04_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1851240.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(14371..15795)
                     /locus_tag="IXH04_000609"
     CDS             complement(14371..15795)
                     /locus_tag="IXH04_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1851241.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            16006..16452
                     /gene="nrdI"
                     /locus_tag="IXH04_000610"
     CDS             16006..16452
                     /gene="nrdI"
                     /locus_tag="IXH04_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAQ1851242.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(16517..18499)
                     /locus_tag="IXH04_000611"
     CDS             complement(16517..18499)
                     /locus_tag="IXH04_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1851243.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDFAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(18513..19409)
                     /gene="murQ"
                     /locus_tag="IXH04_000612"
     CDS             complement(18513..19409)
                     /gene="murQ"
                     /locus_tag="IXH04_000612"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAQ1851244.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(19412..20473)
                     /locus_tag="IXH04_000613"
     CDS             complement(19412..20473)
                     /locus_tag="IXH04_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ1851245.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(20684..21211)
                     /locus_tag="IXH04_000614"
     CDS             complement(20684..21211)
                     /locus_tag="IXH04_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ1851246.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(21278..22780)
                     /locus_tag="IXH04_000615"
     CDS             complement(21278..22780)
                     /locus_tag="IXH04_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAQ1851247.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(23038..24159)
                     /locus_tag="IXH04_000616"
     CDS             complement(23038..24159)
                     /locus_tag="IXH04_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ1851248.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLDFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            24285..>25060
                     /locus_tag="IXH04_000617"
     CDS             24285..>25060
                     /locus_tag="IXH04_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ1851249.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLH"
BASE COUNT         7203 a         5309 c         4446 g         8102 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat
     6661 tttcaagaaa acaaacagaa aattatcata acacaaatta tttataattg aaagactagt
     6721 tttttttcaa gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta
     6781 gtgtcttaag gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa
     6841 aggctggccg caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac
     6901 tcttgtcaat agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg
     6961 tgtattgtgt attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt
     7021 gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga
     7081 acagattgct tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca
     7141 taatatggct tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag
     7201 tactttcctt gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg
     7261 gccgtgggta gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt
     7321 gcgttacaac aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg
     7381 gtatatttca tttacgcatg gatctgcaag tttggcgatg ttcgtgaata gctctgttta
     7441 cttgctgaat gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga
     7501 acgtatgcaa aaaatggaag aagaatacta caacgcacaa caacacgcat aaaaaaactg
     7561 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     7621 aatcagaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
     7681 cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct attttttata
     7741 gtaattcctt cagatacttt cttgcctcat cattgaaacg cagtgattct tctaacttct
     7801 gataatattc caacgcttgt ttctgagctt cagtaaaacg ataatgtttt cctttccatt
     7861 tgtcgattgt ctcttgcaca tttccagatt ggatatcttt cactactagt tgccattttt
     7921 cttttgagac tgtacggtct ttcttatcag cagattgtat agcttctcgt tctttacgca
     7981 atacttttaa aaaaccattt ctgactatcc gaggaatgct gtcgaagaac ccttctgtaa
     8041 aaccatagcg acaatattgc ttccaaataa atagatagat ttttggttgg atgaatcgtt
     8101 ggtttttcgc aaaatattcc atctgagcag tgacttctgg tgttcgttga cgttttgtct
     8161 ttctcaacac ggaaataaat gtttcaaaat aagcattagg atgtactggc acttcttcat
     8221 atacgatgga ttgccaatct ttacgtaaaa atgcgcgata gttgatcaag aattcttcta
     8281 catcctttac taattcttct gcaaagtcgt caaatagttc tttttttatc tgtcgctttt
     8341 ttgactgccc attgatgtgt tcacttattt tcttgatttc attttccaat gcttgataat
     8401 ccgctatgta aatcggcatt tctacttggc gaaattctgc gagccgctga gctaatttta
     8461 tatccggtaa atatggctgt ttcatccgac gattttggta caagacaaaa cagtattttt
     8521 gccatagtcc ttcaggaaaa tcgatattct tctgcttaag ccataataat tcgtctaagg
     8581 ccagatcaac ttttgtcatt ccctgatgga tcgaagctgc cactgtcgga gagacatgca
     8641 ggtgatcggc aaagtggttg atgcctagtt ttaggatctt tccggttttt ggcgatttaa
     8701 cgatgtattg cacttttaat tctctaccac actcacaata caatttttgc tggcttcgtt
     8761 ttgttcgaaa aaacggattg atcttgaagt cagaaaacac ccaatctgtt gcagctaaat
     8821 aatgttcttc cataaaacga gagctgatta gatatcggcg gtgcttatcg actagttccc
     8881 gctgtttttt cgttaatttt gcatagatct cccaacgttg actgcttgtg tattgtttag
     8941 aaattttgat gggatagctt ttgctgattt cttttggaaa ccattcttca aagacggatt
     9001 gccattcttc cacggctgtt tctttccttt ctaattacaa aaagctggct actaacataa
     9061 cgttagcaac cagccagtgt ttcttgctaa attagtgagt tgttccggca tttccggaaa
     9121 tatcttcttt attttctact gctgcttcaa ctgcataagt caaagaagaa gcaatgtctt
     9181 ctaaagacat agaaggcgta cccactggac gatcgatcac ttgatcgggt gagaatggca
     9241 cgtggatgaa cccacctcga attgccggga attgtgtttc gatcaaatga agcaaacgat
     9301 acatgatatc attgcagaca aatgttcctg ctgtataaga gatgtacgct ggtagaccat
     9361 gatcttgtac attttttgtc attgctttga ttggcaatga ggtaaagtaa gcggctggac
     9421 cgttttcttc taaagccgta tcgaatggct gatttccttc attatcaggg attcttgctt
     9481 ctgctagatt gattgcgaca cgttcgaacg agactgctga ccgcccacct gcttgtccga
     9541 cacagatcac gacatctggt tggtgctgtt ggatggcttc tttgactact tgactgcttt
     9601 tttcgaatac ggtcggtact tccactttga tgatttcagc accgctgatt tcatctggca
     9661 ttttttttac tgcttcatag gctggattca ctttgtcccc accaaatgga tcaaaacctg
     9721 ttactagtac tttcatctgt tgttcctccg tccgattgat ttaacatatg gttatctttt
     9781 atcataccat tttccttgaa cagcgtaaat aaaggaaaca atagtttaaa taacataaca
     9841 gatcaaactt aatatattct tataaaaaaa tagtgttcca ctacaatgtt agaagaggtt
     9901 gcgacaatta ttctgtcaca acccctctct tctattattt aaatagaatc atataagcaa
     9961 tttggaaaac tagcataaat aacgcaataa acaactggtt tttgatcact ccgtatttgt
    10021 ctttcatctc cagcatcgct acaggcacga tattgaagtt agcagccatc ggtgtcagca
    10081 gggtcccaca aaaaccgcaa gtcaatgcaa ccattcctat taacgctgga ttcgctccat
    10141 gactaaatac gaatggtgca ccgatcccaa ccgtcatcac ggtgatggcc gcaaatgcat
    10201 tccccatgat taccgtaaat atcaccatgc caagagcgta aatgatgatg ccgatgttca
    10261 catttccttc tggaacgatc gtacctactg cattggaaat cactgtccca actccagcgc
    10321 ttgtaaatac cgcacctaga gaagctaaaa gcattggcaa catgcttaac ggccctactg
    10381 tgcccagcat atctgctgca tcatctaaaa atgtacttgg tcgattatct cgagaaaaga
    10441 acatcaagat tagcatggct gcaaaaacac cgacgcccac gccgactaaa gcacctaaat
    10501 tggtaaatag agcaaacaag atcgcaaaga caccgatact caaagctgga ataaaaattt
    10561 tcattcctag tttgtctgac attttttcca tatattcttt tgatggaagc cggctttctc
    10621 ctttgctgac tttcttagca attgctggta tggtcatcgc aaagatcaag ccaccgttca
    10681 cgattgccgg aagccatctt cctcctgcaa taactacccc taagaccgtc cagaaaaagg
    10741 ctgacccata acgatgagga ttggttttat ctgttaaatt tcttactcca gtgtaaatca
    10801 atacgagtcc catcacgata taaaggatct ccagtaattt atcgcctaat aaaatatcag
    10861 gatttgtaaa aaagtccatc ttattgcccc tttcctggat aatatttctt cgctaatttc
    10921 ttttctttaa gatagaagta gacacttgcc acaactaaag ccgtgatagc cactggaact
    10981 tccactaaag caagctgtcc caatgttacg tcatatccta gatctttcag tgttgattgg
    11041 actagcaacg cccctgctcc tccgatgaat agtacttggc caaagaacca gcagacattt
    11101 tccatagctg aagccatacc ttttagttct tcttcatgct taggatggac ttctaaccct
    11161 tttgattcga tggtccccaa tgccatcggt aaaatgattg gacgaacaaa tccagcaact
    11221 ccaccaaaac tcacgttaaa agccgcaaag atcattcgaa agatcccata aatatctaca
    11281 atcataccag cagatacttt cttgaatcgc ttgatcaagg aagccgctga ttctttcaga
    11341 ccatttcttt caagcgttcc tgttgccaac ataatgatga taaaaatcgc catcccgcga
    11401 ttgtttacaa aacttgtacc taaaagttct aacattcctt gaatgccaag accgccagtc
    11461 aaagctgtaa caactaaagc aaccattacg atcaatatcg agtctaattt aaatgcaaac
    11521 ccgagaataa caatcaagac gcctaaaagg ctaagataat tttccatata ttttcctcca
    11581 taaaatgaca atattttttt aattataaag caaaacacat aaaaaatata gttgttttct
    11641 cacgaaatag acactatttt taaatatttt tcgttatttt tttatcacaa aaattatctt
    11701 acttattgta aggtaaagct ttttttaatt tattgcccga taatatatag aaataaggtc
    11761 tagttactgt tttttttaaa tgattaataa ataacaactt ctttctatga aattgcaaag
    11821 cattcatctt cttttccgct aacgatatat ccgtcagtgc ttggtacact ttactaaaaa
    11881 gtatgagatt gccgatatct ggataggtgt gctatactac agaaaaagat atacaatctg
    11941 ccaccgggta ggataaagag gatgatcatt ccattaggtc tttgaaggga tgattttcct
    12001 ctttttgtgt atttttttat agttaaggag gaatgtaagt atggcatgga tggaattaat
    12061 catagcagga attttggaag tattctggtc aacgatgatg aaatggagtg atggtttttc
    12121 taaaatcaat tattcttttt acacagtgat tggcatgatt gctagttttt actttttgtc
    12181 aaaggcaatc aaaagtttgc cgatgagtct ctcttatcct atctggacag ggattggcgc
    12241 agttggttcg gtcataattg gtgtggtctt gtttcatgat aagctgaatc tttccacttg
    12301 gttctttgtg gggatgctac tcatcagtat cattggcatc aagattacta gcggccacta
    12361 gacacttgga ataagtactg atcagaaaaa ataagccttt ttatttaata ttcaattttt
    12421 ttatgaaaaa ataagaaata agtccaatga cctagcgact tctccactca tcaggtgtat
    12481 gcttgtacta tctatagaaa aggagtgaat atttttgaaa agtctttcgg tccaagaatt
    12541 atccaagaac tttggtcaca aacaagccct cagcgatgtc agttttagct tatcccctgg
    12601 caaaatcgtt ggattagtag gtcctaatgg cgctggaaaa acaacgatca tgaaagctat
    12661 tttaggactg atccattact cagatggaac aatctgtatt ggtgaaaatg agatttctcc
    12721 ctctagtcat aaagggttgg aacaagtagg agcattgatc gaatacccgg gtatctatcc
    12781 attcttaaca ggctatgatc atctgaagtt attctctgaa accaacgatg tatctgctat
    12841 cgacactatc gtgaatcagc tcaagatgga aaagtacatt cacaaaaaag cgaaatccta
    12901 ctcgttggga atgaaacaaa aattgggaat tgctcttgcc ttactgaacc atccgtctat
    12961 cgttatttta gatgagccga tgaacggttt ggatccacaa gcaacaagag atgtgcgaaa
    13021 aatcattacc gacttggcta ctcagggcac ctctttcttg atctccagtc acttactcag
    13081 cgagcttgaa aaaatggtcg atgagctgat cctgatcaat caaggaaaga tcatcaaaca
    13141 atgtacaatg gctgaattga accaaacagc gcaagatttc atcgttatct ctacaacaaa
    13201 tgatctagct gctcgaacga tcttgacaga agctggatat actttggaag atcctgataa
    13261 aatcaagctt ttaaaagaaa acgaagaatt attaggaacc gtgatccagc tattgtctgc
    13321 acaaaatatc catgtgacag acgtccagca tctgacgaat gatttggaat cttctctatt
    13381 ggaaattctg gattcagtaa agggggaaga agaaaaatga cattgcttgt aaaacaagaa
    13441 ttatttaaat tgatcaagaa aaagtcaact gctgttttat ctgttctatt agttgttttg
    13501 ctcataggaa cagcacttct agccaaaaaa tacacgacga tcattgatcc agttgaaatg
    13561 actgctcagc tttttagtgc cacatcttgg attgtcttta tcatgattgc agctgcaagc
    13621 acgatcattt cgatggaagc acaatatggt acattgaaaa atctccttta tcgaaagtat
    13681 tctcgaggag aaatattagt aagcaaatgg atcacattag ttatttattc tgtttattta
    13741 taccttttag ctattattgt cactgtgttg atgaaactga ttcttttccc ttcgatttcc
    13801 ttcacggaga aggtttctac tggtcaaaca ctgatccaaa gcttgttcac ttatactttg
    13861 ggtagttata tcggtttatg gctgatttta agtttagtgt tgatgattgc ttgctttatc
    13921 aatagttccg gcgcatccat ctcagcagga atcgtctttt attttgcttc gtcaatcata
    13981 tctggtatcc ttattgcact catccaaaaa tgggagtgga tcaaatggaa tccgatcagt
    14041 atgttgaatc tgcaaaacca aattggaaac gaagagatca tgaagcaatt gactcatctt
    14101 tctactaatc aaatgctatt cggtaattta gcttacatcg ttttgttctt agcattagga
    14161 tatctcgtat ttaagcgaaa aaatgtatga tgactcatca aaaaagaggc tgtgaaagag
    14221 acgttcagcc ttattgccga ttggctgtct cttaaacacc gtttattctg aaaaaggagc
    14281 tgtgacaaca tttgtgtcac agctcctttc ctagtatgtt tttgaataaa cgacaggctc
    14341 acaacctctc tttatacttc ttttatactt ctatacgtga agcatcccag tggcaaataa
    14401 aaccattgca atcactccga ccatcgtcac acctataagt aaaatcttat ctaagtggat
    14461 atgttgtaca ggctcgtttt gctccttacg tgctttgagg taaaaataaa tgcctggcaa
    14521 gaaaagaaca agaacaagca ttacttcatt aaaggctacg actagcatag cccatacttg
    14581 aaaggcactt gctaaaagcc ctactaaaaa ttggacccat tctttccgct gataagaaag
    14641 tttcatttgg tacaatccta agaaaagcca cgtgaagaaa atagctgctg taccaagtgt
    14701 gtaagcaaag ttataagcac tgtcagtgaa taataatgaa agtaaaaaga cattcgtaca
    14761 taacgtcgtc aatacgatag cataagttgg tgccccattt ttgttgagtt ttccccatgc
    14821 tttaggcaaa agtccgtctt cagccatgtt tcccattgtt tcaatcggta agatcgtcca
    14881 tgagagccaa acaccaacta aagaaatgat caaaccgccg ttgatcacga ctgctcccca
    14941 tttaccaacc atcatttcaa acacatagcc catagcaggc tgcgccttga tcgtttgcag
    15001 ctgattcatc gtcattgtcc catatggaag catggaaacc aagacgtaaa taataatcaa
    15061 gccgaatatc cctagaatcg ttgctttccc aacatctgat tgtttttttg ctcgtgaacc
    15121 tacgacagag gctccttcaa taccaacaaa tacccaaagc atcaccatga tgcaactcaa
    15181 tacttgactg gaaaacggga tcggctgtcc attcgcattc aatataatgt cgctagaaaa
    15241 gttatcaatg aattgttgga tcgaaaacgt gctgatagct actacaataa ataaaaataa
    15301 tggcactaat ttacagaatg tgacgatagc attgacgaaa ctagcatttt cgactccccg
    15361 attcactagc catgcatatc cccataaaaa gactgtactc atcacgatcg atgccagatt
    15421 ttgcccacct tcaaatactg ggaagaaata tcctaatgcg ctcatcaaca atgttgcaaa
    15481 agctacgttg cctaaccagc atgtgagcca gtaaccccaa ccgctgataa aaccagcgaa
    15541 acggccaaaa ccttcttctg cgtaactaaa gatcccgcct tttaaatctg gacgtttttt
    15601 attgatatta ttgatcgaca aggagagcat caacattgct gttcctacga gtacccaact
    15661 gaaaatagct gggccgccag ccgatccgga gatgtccgtc ataataccaa atacgccacc
    15721 tccaatcgct gaaccaatta ctaaggcagc aagtggaatt aaaccaattc cttttttctg
    15781 ttgctctgta tccatgtaag catcctccaa aataaaaccg cttttatcaa aaagtataac
    15841 aaaccttttt tatttttact taattaataa aaactttaat agaaatataa ttctaccggc
    15901 ttttttctca ttatacaagc aaagcgccta gtccctttcc aaattacacc accaaaaaac
    15961 ttaaaaaaga tgctatacta aaaggaaaga attggaggat gatccatgaa cattttatat
    16021 atatcgattt caggaaatac gcgtgccttt gccaaacatc tagcagaata tgctgaaaaa
    16081 atgcatgcag aagatcctgt caatcctgaa gttactttaa aagaaatcca tgaaaatagt
    16141 gattttacga aagaaacaga accatttttt actttcgttc ctacttattt agacggcgga
    16201 aatggtttag ataacggtga tacagaaatc ttgaccgaaa cgatgcgtga atatctggaa
    16261 catgaagaca atcataaact ttgtcttggt gtagtcggta gcggaaataa aaacttcaat
    16321 aatcagtatt gtctaacagc caaacaatat gcacaaacat ttggctttcc tttcttggca
    16381 gattatgaat tacggggaac accatctgat gtgacacgaa tttatcagat tttagcagaa
    16441 aacaataaat aataaaaaag aagaagctga aaaatagatt tcagtttctt ctttcgtttc
    16501 tattgtttct atcatattaa gctttgacat ccatcacagg tgcttttgct tttgctttac
    16561 ctgttttttc caagacaaag ttttttacac gatccatatt ggttaccaag acgatcatgg
    16621 atgtttcttt gcctgctgct ttgatcgctt ctaagtcagc ttctgctaca agcgtatcag
    16681 gtgtcacttg ctgcccttct gttactttca catcaaatgg cgtaccgcct aagtcaactg
    16741 tgttgattcc catatgaagc aagatttcta aaccatttgg cgttgtgatc ccaatagcgt
    16801 gtttcgttgg gaagacagag ctgactaccc cagtaactgg agaatagata tgtccgtttt
    16861 ctggttctac aaagtagcct tcacccatca tcttttgtgc aaaaactgga tcacttgctt
    16921 tatctaaaga aacaactgtt ccgtcagcaa ctgatgagag gatagtcaca ctttcagctg
    16981 aagtttgagg tactactttt tcaggcactt catttactgt ggctgtttca gtcgctaatt
    17041 cttctttttc taaacggtct ttaggaatac cgaagaagaa tgtagcaacg aagccaccag
    17101 cataagctgc caataatcca aggatgtagc ctaaccattt accgtcagag atcaatggga
    17161 tcaaagcggc tcctgaaggt ccaatagcga tagccccgac atttccgatc attccgatca
    17221 ctgccccgcc gatacctcca ccgatacaag cagtaataaa tgggcgtcct agaggtaatg
    17281 ttactccgta aatcaatggt tcgccgattc ctaggatccc gactggtaaa gctcctttga
    17341 tcatttctgt taattcttta tcttttttgc agcgaatcca taatgccaat gctgcaccca
    17401 cttgtcctgc accagccatc gcaagaattg gaagcaatag cgtacgacca gtttcatcaa
    17461 tcatttgcac atggattgga gtcaaaattt gatgcagtcc gaacataacc atcggcaaga
    17521 ataatgtgcc tagaacgaag ccggcaaatg ctccaccaac gccaagtacc cagttgatcc
    17581 caccgaccat gccatcagaa atgaaacctg ctaaaggcat gatgaagaaa atttcaatca
    17641 gaccgatggc aatcaatgaa agagttggtg taacgatgat atctaccgca tcaggtacta
    17701 ctttatgcag ttttttctca acgattgaca atagccaaac agcgaagatg acacctagga
    17761 tcccaccttg tccagctgca agagcttcac cagtaaataa gtttttcagt ggtgcttccg
    17821 gattcatccc tgtcagcatc acaacggccc cgataactcc accaagagtt ggtgttgcac
    17881 caaaaacttg tgcagcattg atacctgtat agataactag gtagctaaac atcccgtttt
    17941 tcaagatgtt catcacatcg atatattgtt gccatgttgc tgcatcaagt gttccggctg
    18001 tgactaagtt tgctaaaact gcggcaatcc ctgccacgat cccagtaccg acaaatgcag
    18061 gaatcattgg cacaaagata ttagaaatgt cttttaatac cgatttgatt ttagatggtt
    18121 tttgtttcga tttttgtgcc gctttcattt cttgtgcccg ttcgtttaca cggtcttttc
    18181 cagatgctga tgaatttgct gtagctgtac ttacagaaga aagtttttct cctaatttca
    18241 cgcctgcaaa atcaaccatt tgctgtgcta ctttgttcac tttcccaggt ccgatgatga
    18301 cttgaagctg ttcatcttct actacaccaa gcactcctgg aatcgctttt aattcagaga
    18361 catttacaag atcatcattg atgatcttca tacgcacacg agtcatgcag tggacaatgc
    18421 tttctacatt gcccatgccg cctgcccctt tataaatttg ttttgccagt tcttccaaag
    18481 tcagttcgtt ttttgccatg gtcttttccc cctcataagg tcccttttac aaatccgttt
    18541 gcttcagcta attttgcttg agcttcctct ttatcactat ttgtcaaaat catgacgata
    18601 gccagcttaa cattttgatc tgcttcttca aagtactttt cagctgtttc gtaatcagct
    18661 tctgtcgctt gcatgatgat tcgttttgag cgttcgatta gtttttcatt tgttggtttg
    18721 acatcgacca tcaagttatt gaatacttta cctataccaa tcatactgat agtagaaatc
    18781 atattcaaga taagtttttg tgctgttcct gacttcaatc gagtagagcc tgtcaaaaat
    18841 tctggacctg catcgacttc gattgggaaa tccgcgtgtt tactgatttc ggcttgttta
    18901 ttgcaagaaa tcgttcctgt gacagcaccg atcgattttg cgtaatctaa tccaccgatc
    18961 acatatggtg tacgaccgct agctgcgata ccaataacta tatcattttt tgtcaaattc
    19021 agcttgatta gatcttcttg ccctagctca taagaatctt cggccccttc gacagctaca
    19081 gtcattgctt cttctccacc agcaattaga cctatgacca tttccggttc tacaccgaat
    19141 gtcggtacgc attcagcagc atctaacaca cctagtcttc cacttgttcc ggctcccata
    19201 taaatcagac gtccaccttt tttaaaggac tcaatcgtgt tagcaatcac tggttcgata
    19261 cttgtcagtt ctttttcaac cgcagcggcc actttctgat cttctttgtt cattagttga
    19321 acggcttgag aaatactcat ctcatctaat ccaaaagttt cttggtttct gcgttccgtt
    19381 gttaggttct ctaaattgat tttgctcatt tttatcttcc ttcttttctt ataaattgat
    19441 agttcatacc agctttgatt tggttgatca atggtagctc ttctttgata actttagcta
    19501 ctacattcac tttttcatcc gctggcaaat catatttggt taattggatc tcacccatat
    19561 agcgcaaata cttttcgtta tctaaagtaa tagacccttt catacgcgta gctgtatttc
    19621 ttgctgggat aggcggaatc tctcgaaaac gtgcatctac gcttcgtatg acatctcttg
    19681 catcgtcttg ccgattcgta tgcttcccta atatatattc gtaaaattct tcatctacag
    19741 gttccgtatg gagcaagact gtctgttctt tttggaaaga agcaaattgc tcctgaacgt
    19801 tttctgacag acctgcatct ccgatgtaaa caatatccgt gttacttgct aatagatcta
    19861 gagcgcctgc aagaggatgg atccctcgat gttcttccaa agttggcaat ccttgatata
    19921 gtgggcctct caactctgta tctcctggta caaaaccttg gacaaggaga ccttgaattt
    19981 tgagccattg attttttttc tgataccatt ctttgtccag ccctgtttcg ggacgtggat
    20041 aataattatg ccaagcttca atctgtgaga aatcagcttc tgcttctttc aattcatcga
    20101 tatctttagg cgtgatcgtg ctagcattca aactgatttt caattgatgt gaccagtcgg
    20161 caatctgctg attggaaata tgataatcca ttcttaaacc tgttacacca attgctttca
    20221 aaggcgttag atcttctaat gaaaaacccg ctcgttccaa tgcctctcct gaaatatcga
    20281 ccatcaattc tagtttattc tttttcgctt cgcttcctag ttcgatcaat cgcttttgat
    20341 atgccgaaac atcatcttct ggtatatgca tggaagtgaa aataccgaca aagccattgg
    20401 atgccatttg gcggatatac tgtttcttct cttctgacaa agaatcgttc ataaatattg
    20461 aaaagccaaa catattatcc ctctttttct gtatccgtct tcatttctat gactagagta
    20521 tatatctaaa gaaacaaaat ttcaatactt ttattaaaat aattaaattt catttctaaa
    20581 ggttgagttt atattatcaa aagaattgtc ataacctaaa aaaggcactg ctaagcaaaa
    20641 gcagtgcctt gaaatatttt atttaaataa cacaaaaaca taattactca taagaaacca
    20701 attctgtatt atctaccatg aatgtaaaga acccctcatt aggcatcgga tgaaaataag
    20761 cattgatcac tcttatcaag gcattatggc aaactaacag atagacattt tcttcgtctt
    20821 cgatgcattc tttcaataaa ggaacgatcc gtgcatacac atctaagact gactccccat
    20881 ttgggaatcg tacagcgaac gctaaacgtg ctttttgaaa gttctcatct ttgcttggca
    20941 gtccatcata atccccaaaa tccatttcta ccaatcgttc atcaacggtc agcggcaagc
    21001 tgagacgatc agcaactgct tgtgctgtat ctcttgctcg ttgaagaggg gaatggatga
    21061 ttttcgtgat tggaacaggc aaatctacta ccttttctgc tagtttctgt gcctgcatct
    21121 gtcctttttc agtcagcggc acatctgccc gtccgcaaac taatccttgt gcgttccaag
    21181 ttgtttctcc gtgtctcgtt acataaagca tgtgattctc tccttcgttg gcaaagttcc
    21241 ccttaaccat accttgttgt tcttgagatt acaagggtta gattctctca agagaaataa
    21301 tctcagtaga tactttatcc aggaaggttt gatcatgttc cactagtaac atggcaggtt
    21361 tcacgttcaa gatcagttgt tctaattgtt cttgattgaa gacatccaaa taattcaatg
    21421 gttcatccca tgtatatagt tcagctggct gtgataaaga tttagccaat tccacttttt
    21481 tccgttggcc catactcatc tgctcgatct tgttatgaaa aacatctctt tccatcccaa
    21541 gctttcggag gttgttcaaa aatgcttggt agtctacttg gtttttctct gcaaattccg
    21601 ccaacgttcc tcgattgtct tcataatttt ggcttgcata actaatgctc agatgttttg
    21661 gctggtattt ttctcctata accttgccgt tgaatgcccc cagaagataa tggataatgg
    21721 atgactttcc tgcaccgtta ggacctgaaa tcgccacccg ctgatgaggc tcgattgtaa
    21781 aatgaattgg ctcgaataac agattttcat accctaattg aagatcttct acgcttaaaa
    21841 gtcgcttatg gtgagacgct tggctattca tcgtcaaaga atcgatatat tcgatatctt
    21901 ttaatagttt ttctttctcg ctgatctggg tctccatccg attcacgatt gctttggatc
    21961 gtttcatcgt ccgtgcagca tcagcaccca ctgctccttt attcactcgt ctagattcag
    22021 tatcgatgaa tccgacttgt ttctttgttt tatctccttc tcgggaacga gaccattcgg
    22081 ctttttcagc tgctgttttc tttagcctac tgacttcttt tttcaatttt tcattttgag
    22141 ccatttcaaa ttcatcacga agttttttct gttcttcata gatagagaaa ttcccttgat
    22201 aaagttccag ttgacttttt tcgattgcta aaacatggtc cactacttca tcgataaatc
    22261 cccggtcatg gctgaccacg atgaagcctt gttttttctt tttcaaataa gccgctactt
    22321 gttttctacc agagatatcc aaatgattcg ttggttcatc gattaacggg aaatgagtgt
    22381 catccacaaa taaaagtgcc agtaggactt ttgtcttctc tccccccgat agtgtgctga
    22441 attctctcca taagatttca ggatctgttt gcatcaattg gagctctctt tcgatttccc
    22501 atatctcaaa atccgtaata tcatttaaca cgtaataggt taaacgttct ttatcttttg
    22561 tctgttgcgg gaaataggca aattcttgct gatggattac ttgcccttga taaggtagtt
    22621 tgttttgtag aatattcagt aaagttgtct ttcctcgacc gtttcgtccg ataagtccta
    22681 gtttccattg tgtgtcaaaa tttagatttg cttgttcaaa taataatgtg ccttggctgt
    22741 cgtagccgaa tgtcagattt tttatttcga ttttagacat tttccatcac ctcgtaaaaa
    22801 aagaccagtc ctattttcag aactggctaa agaggatgca agggttggat acacacaaaa
    22861 agacccatac atcgaaaaaa ccaagccaat cccgaaaatc tgcacactgc ttccttgtct
    22921 ccacaaggta cagtctgaat gcagctttca tcaatcgact taatttttca atgcacggat
    22981 ccctcacttt gtcctatatt tgaaattacg atagcataaa gaaaatgatt ttgtcaacta
    23041 cttcgggagg atggtgaaaa atctgctaag tgtctgtcct aagatttttg ttttttctaa
    23101 tgtttgcctg tgttccattg cttcttctaa acctattgga cccaaatgaa tacttaagac
    23161 aattgggaga aaatcagaaa gtccttctag ctcttggtct ctacttccgc aaatagcgat
    23221 tattggtttt cgatattttt ttgcaagcac tgctacaccg tatggaactt ttccgtggat
    23281 cgtttgctga tcgatccgcc cttctcctgt aatgatgaca tctgcttgtt cgatcttttt
    23341 ttccaatcca gtgatttggc tgactaaatc gaaaccagaa accatcttcg ctcccaacaa
    23401 aaaaagggct ccaccaatac cgcctgctgc tccacttcct ggaatatcat ctaaatccac
    23461 ctcccagaaa tcttttattt gctgagcaat ctttcctgct tgtttatcta agaaatctag
    23521 cgtcgtatct attcctcctt tttgtcttcc aaattgccaa gcagctcctt gttgacctgt
    23581 atatggattg gtaacatctg cagcgataat caattttact ttagaaacaa gaacgtttct
    23641 tcgatcaaaa tgaagttttt tagtagataa taaaggattt ccttctgtgt acccttctaa
    23701 agaagcaccc aaggattgga gtaatcctag accaccatcg ctacacccgc ttcctcctaa
    23761 agtgacaata atttgttcta cttcttgaag cagtgcatct ttaataagtt ctcctaaggc
    23821 ataactggaa gcaagtcgga tagtttgatc cgttggttta tcaagtagat ctaaccccaa
    23881 tgcttgtgca gattcgatga ctgcggtgtt ttttccttgc caagttgtca ataaatagct
    23941 gatttttcgc tgacggaata agagatccat cccatcaatg gtacgccatg ttcccctgag
    24001 cgcgtaatag attgcttctg cagtattttc tccgccatct gcaatcggca cattgatttt
    24061 tcttccttta cgatcttccc agccatttag aactgcctga tttagttcaa atgaagaagc
    24121 acttccttta aatgaatcaa ttgccgccaa aatatccatc tgtatccctc actttcttct
    24181 taagtaagac actttttttc atttttttca aacaaaacaa tgctattttc ttccattttt
    24241 ctatatacta aaagggaact cattgaataa aggagagaaa aaacatgaaa atcgcaatag
    24301 ctggggccgg agcaatgggc agccgaatcg gattgatgct acatcaaagc ggaaatgagg
    24361 tcctcttgat cgatcgttgg cctgctcaca ttgaagccat tcgtacaaat gggttgattg
    24421 ctgactttaa cggtaaagaa gtggtcgcaa aactgccaat ctattcacca gaagaaatca
    24481 tcgaaagcaa cgaacacgtg gacttgatcg tagcattaac aaaagccaat caattagatg
    24541 atatgttttg ttcgatccaa tccattatta ctgacaatac ttatgttttg tgtttactaa
    24601 atggattagg tcatgaagat gtactggaaa aatatgtgcc aaagaaaaat atcttatttg
    24661 ggatcacgat gtggacagct ggtctagctg gtcccggaaa agtcacattg ttaggtgatg
    24721 gagaaatcga actagagaac ttggaaccag aaggcgaagc atttacaaaa aaagttgtag
    24781 aagtctttca agaagcaaat ttaaatccga tctatagcca taacgttcgt tattccattt
    24841 ggcgaaaagc ttgtgtaaat ggaacattga atggtttatg cacgattttg gattgtaata
    24901 ttgctgaact aggcgcgcaa aaagcggcag agagcatggt tcgaacaatc gtatctgaat
    24961 ttgcttctat cgcagcaaaa gaaggaatca tactagatca agaagaagtg taccaacata
    25021 ttgcttctac ttatgatcct gacaacatcg ggttgcatta
//
LOCUS       DACEXQ010000020        21989 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.020, whole genome shotgun sequence.
ACCESSION   DACEXQ010000020 DACEXQ010000000
VERSION     DACEXQ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21989
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.020"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            11..358
                     /gene="rplV"
                     /locus_tag="IXH04_000618"
     CDS             11..358
                     /gene="rplV"
                     /locus_tag="IXH04_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAQ1851250.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            372..1028
                     /gene="rpsC"
                     /locus_tag="IXH04_000619"
     CDS             372..1028
                     /gene="rpsC"
                     /locus_tag="IXH04_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAQ1851251.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            1031..1465
                     /gene="rplP"
                     /locus_tag="IXH04_000620"
     CDS             1031..1465
                     /gene="rplP"
                     /locus_tag="IXH04_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAQ1851252.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            1455..1643
                     /gene="rpmC"
                     /locus_tag="IXH04_000621"
     CDS             1455..1643
                     /gene="rpmC"
                     /locus_tag="IXH04_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAQ1851253.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            1668..1934
                     /gene="rpsQ"
                     /locus_tag="IXH04_000622"
     CDS             1668..1934
                     /gene="rpsQ"
                     /locus_tag="IXH04_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAQ1851254.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            1992..2360
                     /gene="rplN"
                     /locus_tag="IXH04_000623"
     CDS             1992..2360
                     /gene="rplN"
                     /locus_tag="IXH04_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAQ1851255.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            2397..2705
                     /gene="rplX"
                     /locus_tag="IXH04_000624"
     CDS             2397..2705
                     /gene="rplX"
                     /locus_tag="IXH04_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAQ1851256.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            2733..3272
                     /gene="rplE"
                     /locus_tag="IXH04_000625"
     CDS             2733..3272
                     /gene="rplE"
                     /locus_tag="IXH04_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAQ1851257.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            3291..3476
                     /locus_tag="IXH04_000626"
     CDS             3291..3476
                     /locus_tag="IXH04_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAQ1851258.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            3512..3910
                     /gene="rpsH"
                     /locus_tag="IXH04_000627"
     CDS             3512..3910
                     /gene="rpsH"
                     /locus_tag="IXH04_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAQ1851259.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            3942..4478
                     /gene="rplF"
                     /locus_tag="IXH04_000628"
     CDS             3942..4478
                     /gene="rplF"
                     /locus_tag="IXH04_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAQ1851260.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            4639..4995
                     /gene="rplR"
                     /locus_tag="IXH04_000629"
     CDS             4639..4995
                     /gene="rplR"
                     /locus_tag="IXH04_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAQ1851261.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            5016..5516
                     /gene="rpsE"
                     /locus_tag="IXH04_000630"
     CDS             5016..5516
                     /gene="rpsE"
                     /locus_tag="IXH04_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAQ1851262.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            5531..5710
                     /gene="rpmD"
                     /locus_tag="IXH04_000631"
     CDS             5531..5710
                     /gene="rpmD"
                     /locus_tag="IXH04_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAQ1851263.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            5754..6194
                     /gene="rplO"
                     /locus_tag="IXH04_000632"
     CDS             5754..6194
                     /gene="rplO"
                     /locus_tag="IXH04_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAQ1851264.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            6194..7489
                     /gene="secY"
                     /locus_tag="IXH04_000633"
     CDS             6194..7489
                     /gene="secY"
                     /locus_tag="IXH04_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAQ1851265.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            7551..8198
                     /locus_tag="IXH04_000634"
     CDS             7551..8198
                     /locus_tag="IXH04_000634"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAQ1851266.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            8389..8607
                     /gene="infA"
                     /locus_tag="IXH04_000635"
     CDS             8389..8607
                     /gene="infA"
                     /locus_tag="IXH04_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAQ1851267.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            8640..8756
                     /gene="rpmJ"
                     /locus_tag="IXH04_000636"
     CDS             8640..8756
                     /gene="rpmJ"
                     /locus_tag="IXH04_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAQ1851268.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            8774..9139
                     /gene="rpsM"
                     /locus_tag="IXH04_000637"
     CDS             8774..9139
                     /gene="rpsM"
                     /locus_tag="IXH04_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAQ1851269.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            9167..9556
                     /gene="rpsK"
                     /locus_tag="IXH04_000638"
     CDS             9167..9556
                     /gene="rpsK"
                     /locus_tag="IXH04_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAQ1851270.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            9636..10574
                     /locus_tag="IXH04_000639"
     CDS             9636..10574
                     /locus_tag="IXH04_000639"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAQ1851271.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            10602..10982
                     /gene="rplQ"
                     /locus_tag="IXH04_000640"
     CDS             10602..10982
                     /gene="rplQ"
                     /locus_tag="IXH04_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAQ1851272.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            11254..12093
                     /locus_tag="IXH04_000641"
     CDS             11254..12093
                     /locus_tag="IXH04_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1851273.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            12069..12938
                     /locus_tag="IXH04_000642"
     CDS             12069..12938
                     /locus_tag="IXH04_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1851274.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            12928..13725
                     /locus_tag="IXH04_000643"
     CDS             12928..13725
                     /locus_tag="IXH04_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAQ1851275.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            13928..14674
                     /gene="truA"
                     /locus_tag="IXH04_000644"
     CDS             13928..14674
                     /gene="truA"
                     /locus_tag="IXH04_000644"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAQ1851276.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(15007..17676)
                     /locus_tag="IXH04_000645"
     CDS             complement(15007..17676)
                     /locus_tag="IXH04_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAQ1851277.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(17914..18102)
                     /locus_tag="IXH04_000646"
     CDS             complement(17914..18102)
                     /locus_tag="IXH04_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAQ1851278.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            18372..18734
                     /locus_tag="IXH04_000647"
     CDS             18372..18734
                     /locus_tag="IXH04_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ1851279.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            18786..20468
                     /locus_tag="IXH04_000648"
     CDS             18786..20468
                     /locus_tag="IXH04_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAQ1851280.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            20585..>21989
                     /locus_tag="IXH04_000649"
     CDS             20585..>21989
                     /locus_tag="IXH04_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAQ1851281.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYV"
BASE COUNT         7255 a         3960 c         4776 g         5998 t
ORIGIN      
        1 ataaatccaa atggcagaac aaatcacatc agctaaagca actgcaaaaa cagttcgcac
       61 ttcacctcga aaagcccgtt tagtaattga ccttatcaga ggtaaaagtg tcgcagatgc
      121 aatttcaatc ttgaaattca caccaaacaa atctgctgga atcattgaaa aagtattgat
      181 gtcagcagtt gcgaatgcag aaaataactt tgacttagat gttgaaaact tggtagtaag
      241 cgaagctttt gttaacgaag gacctacaat gaaacgtttc cgtcctcgtg caaaaggatc
      301 agcttctcca atcaacaaac gtacaagtca tatcacggta gtcgtatcag aaaaataagg
      361 agggataatc agtgggtcaa aaagtacatc caattggaat gcgtgtaggc atcatccgcg
      421 actgggatgc taaatggtat gctgaaaaag agtatgctga gttcttacac gaagatttaa
      481 gaatccgtaa atttatttca actaaacttg ctgatgctgc tgtatcaaca gttgaaatcg
      541 aacgcgcaac aggtcgcgta aacatttcaa ttcacacagc taaaccaggt atggttattg
      601 gtaaaggcgg ttctgaagtc gaaagcctaa gaaaagaatt aaacaaatta actggtaaaa
      661 gaatccatat caatatcgtg gaaatcaaaa aaccagatct agatgcaaaa ttagtaggtg
      721 aaggaatcgc acgtcaatta gaaaaccgtg ttgctttccg tcgcgctcaa aaacaagcca
      781 tccaacgctc aatgcgtgct ggcgctcaag ggatcaaaac tcaagtatct ggtcgtctaa
      841 acggagcaga tatcgctcgt tcagaaggat actcagaagg aactgttcct cttcatactt
      901 tacgtgcgga cattgattac gcatgggaag aagcagatac aacatacgga aaactaggag
      961 ttaaagtgtg gatctatcgt ggagaaattc ttccaactaa aaaaaacact gagaaaggag
     1021 ggaaataatc atgttagtac ctaaacgtgt aaaacaccgt cgtgaattcc gcggaaaaat
     1081 gcgcggggaa gctaaaggcg gaaaagaagt agcattcggt gaatacggtt tgcaagctgt
     1141 tgattcacat tggatcacaa accgccaaat cgaagctgct cgtatcgcaa tgactcgtta
     1201 catgaaacgt ggtgggaaag tatggattaa aattttccct cacaaatctt atactgccaa
     1261 agcaattggg gtacgtatgg gttctggtaa aggggcacct gaaggatggg ttgcaccagt
     1321 aaaacgtggt aaaatcatgt ttgaaatcgc aggcgttcct gaagaagtag ctcgtgaagc
     1381 gttacgtcta gcttctcaca aattaccaat gaaaactaag atcgtaaaac gtgaggaaat
     1441 gggtggtgaa tcgaatgaag gttaaagaaa tcagagaatt aaccactgcc gaaatgcttg
     1501 atcaagaaaa acaattaaaa gaagaattgt ttaatcttag attccaatta gcaacaggtc
     1561 aattagaaaa cactgcacgt attaaagaag tacgtaaatc gattgcacgc atcaaaactg
     1621 tgttgcgtga acaagctaag taattatgga aggaggccat taagcgtatg actgaaggaa
     1681 gaaatcaacg caaagtttat caaggtcgcg tggtttcaga caaaatggat aaaacaatta
     1741 ccgttgctgt cgaaacaaag aaaaaccacc ctatctatgg caaacgcatg aactattcta
     1801 agaaatacaa agtgcatgat gaaaacaaca cagcaaaagt tggcgacatc gtgagaatta
     1861 tggaaactcg tccattatca gctacaaaac gtttccgttt gttggaagta gtcgaagaag
     1921 cagttattat ctaataacac gagattttcc tatatagatt gaatgcaaaa tctgaaagga
     1981 ggatacacaa cgtgatccaa gcagaaagtc gtttaaaagt cgctgataac tcaggcgcac
     2041 gtgaaatttt gacgatcaaa gtccttggtg gatctggtcg taaaactgct aacatcggtg
     2101 atgtgatcgt tgctacggtt aaacaagcaa cgccaggtgg ggttgtcaaa aaaggtgacg
     2161 tcgttaaagc cgttatcgtt cgaacaaaat caggagctcg tcgtacagac ggttcatata
     2221 ttaaatttga tgaaaatgct gcagtgatta ttcgtgatga taaaagccct cgtggaacac
     2281 gtatctttgg gccagttgca cgtgaactac gtgaaaacaa cttcatgaag atcgtttcac
     2341 tagcaccaga agttttataa tcttgagaca cttatcaaag gaggtgcgaa acagttatgt
     2401 ttgttaaaaa aggcgataaa gttaaagtta tcactggaaa agacaaaaac aaagaaggcg
     2461 ttgtactagc agcgttcccg aaaaaagata aagttattgt cgaaggtgta aatgtcgtta
     2521 aaaaacacca aaaaccttct caagctgctc ctcaaggtgg aattgttgag atggaagcgc
     2581 caattcatgt ttctaacgtg atggtggttg actctactgg tgtagctggc cgagttggct
     2641 acaaagaagt agacggcaaa aaagtccgtg tttctaaaaa aaccggtgaa gttttagata
     2701 aataaaagtt taaggaagga gggtcttact gaatgaaccg cctaaaagaa aaatatctta
     2761 aagaagtaac tccatcattg atggaaaaat ttaactatag ctctgttatg caaacaccta
     2821 aagttgaaaa aatcgtcatc aacatgggtg ttggtgatgc ggtatcaaac gctaaaaact
     2881 tagataaagc agttgaagaa ttaactttga tcactggtca aaaaccaatg attacaaaag
     2941 ctaaaaaatc aatcgctgga ttccgtttac gtgaaggaat gccaattggt gcaaaagtta
     3001 cattgcgcgg agaaagaatg tacgaattct tagacaaatt ggtttctgtt tcactaccac
     3061 gtgtacgtga cttccatggt gtaagtaaaa aagcctttga cggtcgtgga aactatactt
     3121 taggtattaa agaacaattg atcttcccag aagtagatta tgatttagta gataaagtac
     3181 gtggtatgga catcgttatt gtaacaacag caaactctga tgaagaatct cgtgagttgt
     3241 tgacacaatt aggcatgcca ttccaaaaat aattaaagga ggcgaactac gtggctaaaa
     3301 aatcaatgat tgctaaaaac aaacgtcctg caaaattttc aacacaagct tatactcgtt
     3361 gcgaacgttg cggacgtcca cattcagttt atcgtaagtt ccatctttgc cgtatttgct
     3421 tccgcgaact tgcctataaa ggtcaaattc ccggcgtgaa gaaagctagc tggtaaaaag
     3481 taaattcgca taagaaggag gtaaaagatc aatggtcatg acagatccaa ttgcagattt
     3541 tctaactcgt atccgtaacg caaacatggt taaacatgag tctttagaag tacctgcatc
     3601 aaaaattaaa cgtgacatcg ctgaaatctt gaaacgtgaa ggtttcgttc gtgatgttga
     3661 atacatcgaa gatgacaaac aaggcgtgat ccgtgttttc ctaaaatttg gtaaaaacgg
     3721 cgaacgtgtt atcacaaact taaaacgtat ctctaaacca ggtttgcgtg cttatgtcaa
     3781 agctgacgaa gtaccaaaag tattgaacgg tttaggtatc gcaatcattt cgacttctga
     3841 aggtgttgtc actgataaag aagctcgtga aaaaaatatc ggcggcgaag ttatcgctta
     3901 cgtatggtaa tcaagtagaa tctacaagga ggtgtcttta agtgagccgt atcggaaata
     3961 aagtcgttgt tatccctgaa ggagtaacag ttactcaaga cggaaacaac gttacagtta
     4021 aaggaccaaa aggtgaattg actcgtactt tttcagcaga tatcaaaatg aatatcgaag
     4081 gaaacgaagt aactttcact cgtccaaacg acagtaaaga aatgaaaaca attcatggaa
     4141 caactcgtgc gaacttcaac aacatggttg taggtgtcag cgaaggtttc caaaaagcgt
     4201 tagaactaat cggggttggg taccgtgcac aattacaagg atcaaaactt gtattgaacg
     4261 ttggttactc acatccagta gaaatcacac caccagctgg agtaactgtt gaagtaccag
     4321 ctaacacaca agtaattgtt aaaggtgcga acaaagaaga agttggcgaa ttagctgcaa
     4381 acattcgtgg aactcgtcct ccagaacctt ataaaggtaa aggtattcgt tatgttggcg
     4441 aatatgtacg ccgtaaagaa ggtaaaactg gtaaataatc gtttagaaaa attgcataga
     4501 agccagcagc aggaaaaagg catttggctg aagtttacct tcaaaaaaat acatttttac
     4561 ttgtgccgca gcttctattt ttcgaaacgt atttactgct tgaaatagta gtacaataaa
     4621 aataaagagg tgacaattgt gattacaaaa ccagataaga acaaaacacg tcaaaagaga
     4681 catcgtcgtg tacgtaacaa tatctctggt actgctgagc gcccacgctt gaacgttttt
     4741 cgttctaaca aaaacatcta cgctcaaatt attgatgacg tagcgggtgt aacgctagca
     4801 agtgcctcta ccttggataa agaaatttca ggtggaacaa aaacagaagc agcacaagct
     4861 gtcggtaaac tagttgctga acgtgcagct gaaaaaggca ttaaagtagt agtctttgac
     4921 cgtggtggat acctttacca tggccgtgtg caagctttag ctgaagctgc tcgcgaaaat
     4981 ggactagaat tttagaagaa ggaggaatac cattcatggt ttatatcgat ccaaaacatt
     5041 tggaattaga agaccgcgtt gttgcaatta accgtgtaac aaaagttgtt aaaggtggac
     5101 gtcgtctacg ttttgctgct ttagttgttg tcggtgacaa aaacggacac gtagggtttg
     5161 gtacaggtaa agctcaagaa gttcctgaag caatccgtaa agcaattgaa gatgcgaaga
     5221 aaaacttagt tgaagtacct atggttggtt caacaatccc acacgaagta atcggtgctt
     5281 ttagtggtgg tagaatccta atgaaacctg ctgtagaagg ttctggggtt gctgctggtg
     5341 gaccagttcg tgccgtatta gaattagctg gggtagcaga tatcacttct aaatctttag
     5401 gctcaaatac accaatcaac gttgttcgcg caacagttga aggtttgaaa caattgaaac
     5461 gtgcagaaga agttgcagaa cttcgcggca aatcagttga agaattaatc ggataaggag
     5521 gacaacaata atggctgaat taaaagttac tttaaaacgc agtattatcg gacgtcctca
     5581 aaaccaacgc gatacagtta aagcgttagg tcttacaaag attaacagtt cagtggttaa
     5641 gccttctaat gaagcaatta aaggcatgat caatactgtt tctcacctag tggacgttga
     5701 agaaatttaa tttagaaaaa tcataactag aacattaagg aggtgccaaa cctatgaaac
     5761 ttcatgaatt aaaacctgct gaaggttcac gccaagtacg taaccgtgtt ggtcgtggta
     5821 cttcatctgg taacggaaaa actgccggac gcggacaaaa aggacaaaaa gctcgttcag
     5881 gcggcggtgt gcgtttagga tttgaagggg gacaaacacc attgttccgt cgtttaccaa
     5941 aacgaggatt cacaaacgtg aaccgtaaag actatgcagt cattaatctt gatgtattga
     6001 accgctttga agacggagct gaagtaactc ctgttgcttt agttgaagct ggaatcgtga
     6061 aaaacgaaaa agctggaatc aaagtattag ctaatgggga actaaacaag aaattaactg
     6121 tgaaagcagc aaaattctct aaagcagctc aagaagcaat tgaagcggct ggtggatcaa
     6181 tcgaggtgat ctaatgttta gactattaaa agatgctttt aaagtaaaag acattagatc
     6241 gaaaatccta tttactgtat ttgtgttgtt tgtatttcgc ctaggtgcac atattactgt
     6301 accaggcgta aatgctgctt cattgcaaaa cctttcttct ttgccatttt tcaacatgtt
     6361 gaacttggta agtggtagtg cgatgcaaaa cttttccatt ttctcaatgg gggtttcacc
     6421 atacattact gcttctatcg tcattcagct tcttcaaatg gacatcgtgc ctcgatttgt
     6481 tgaatggtcg aaacaagggg aagttggtcg taaaaaactg aatcaagcga ctcgatatct
     6541 gacattagta ttggcattta tccaatctgt cggtatcact gctggattca atcaatacgc
     6601 tcaattaggt ttcgtgaata atcctaacgt agcaacttac gtaacgatcg gtttgattct
     6661 aacagccggt acgatgtttg ttacttggct aggagaacag atcacagaaa aaggtatcgg
     6721 aaatggcgta tcaatgatca tttttgcagg tatcatttct agattgcctg aaagtatcag
     6781 agaaatttat gaagattact ttataaatat tgatccttca gacatatgga aatctgctat
     6841 ttttgtggcc attttactag tagcggtctt gttgatcgtg acattcgtaa catttttcca
     6901 acaagctgaa cgcaagatcc cgatccaata tacgaaacgc gtagctggcg cgcctacaag
     6961 cagctatctg ccgttgaaag taaatgcagc tggagttatt ccagttatct ttgctagttc
     7021 gtttattgct acgccaaatg ctgttttaca agcattgagc ggtagattcg gttctgaaaa
     7081 ctggtacaaa gtgatgatgg agatctttaa ctataataca gcacctggtg ctacgattta
     7141 tacgattctg atcgttgcct tcactttctt ctacgccttt gttcaagtaa accctgagaa
     7201 attagcggaa aacttgcaaa agcaaggaag ttatatacca agcgtacgcc ctggtaaggg
     7261 gactgaagag tatgtctcaa gattactgat gcgtttaagt acagttggtg cagtcttcct
     7321 tggtttagtg gcattgcttc caatcattgc tcaaatggtt tgggatcttc cacaatcaat
     7381 cggattaggc ggtacaagct tactgatcgt tatcggtgta gctttagaaa ctgcgaaaca
     7441 gttagaaggc ttaatgctga aacgtaaata tgttggattc attgagtaag aaagcgtgtt
     7501 gaggtttccc ctcaacacac gttctcacac atttattagg agggaaaaca atgaacctca
     7561 ttttaatggg gctgccaggc gcaggtaaag gaacacaagc tgaaaaaatc attgatgctt
     7621 atggtattcc gcacatctca acaggagata tgttccgcgc agcgatgcaa aacgaaacag
     7681 ctcttggcct ggaagcaaag tcttatatgg ataaaggtgc attagttcct gatgaagtaa
     7741 caaatggaat cgtgaaagag cgactagctg aaccagatac agagaaaggc ttcttattag
     7801 atggctttcc acgtacgcta gatcaagcaa aagctctgga cgcaatgcta aaagatttga
     7861 ataaaaaaat tgatgctgtc atcgatatcc atgttggtga agaaatcttg gtcgaacgtc
     7921 tagccggccg atttatctgc agcaattgtg gagcaactta ccataaaatt ttcaacccta
     7981 caaaagttga agacacatgt gatcgttgcg gtgggcatga attctatcaa agagaagatg
     8041 ataaacctga gacggttaaa aatcgtctgg ctatcaacat caaaaatagt gaaccaatct
     8101 tggcttatta taaagaacaa ggtttgctaa acactattga tggtgctcgt gaaatcgatg
     8161 ctgtatttgc agatgtacaa aaaatcattg aaaaatagca cgattgcccg tagccgactt
     8221 gcattatgca gttactcttg tcaagttcaa taaattatga tagaatatgt cagtccgact
     8281 tctagagaat cctctagatg atccattttg agaattttga ggtgggaact gcagtttccg
     8341 ccattttatc gtgtgaaaat gcgaaacaag tacaaggagg tactgtgcgt ggcaaaagaa
     8401 gatatgattg aagtcgaagg tacagtcgtc gaaactttgc cgaatgcaat gtttaaagtt
     8461 gaactagaaa acggacacca agttcttgct actgtttcag gtaaaattcg tatgcattac
     8521 atccgtatct tacctggtga caaagtaacg gttgaattgt ctccatacga tcttaaccgt
     8581 ggccgtatta cttatcgctt taaataattg tactccgtaa aactacagga ggtattatca
     8641 tgaaagtaag accatcagta aaaccaatgt gcgaacattg taaagtaatt cgtcgtaaag
     8701 gacgcgttat ggtgatttgc ccagcaaatc caaaacataa acaacgtcaa ggataattgg
     8761 aggtgctata aatatggctc gtattgcagg agtagatatt cctcgtgata aacgtgtagt
     8821 agtttcactt acttatatct atggtattgg taacactact gctaaaaaga tcttagctga
     8881 tgctggtgta tctgaagatg ttcgcgttcg tgaattaacg aatgaacaaa cagatgctat
     8941 ccgtgcagaa gtagataaat taaaagttga aggggatctt cgtcgtgaag tgaacttgaa
     9001 catcaaacgt ttgatggaaa tcggttctta ccgtggaatc cgtcatcgcc gtggattgcc
     9061 tgttcgtgga caaaacacga aaaacaatgc acgtactcgt aaaggcccag ctcgcgctat
     9121 cgctggcaag aaaaaataat tcttaagtga aggaggttaa aacttcatgg cagcaaaaaa
     9181 agtgaatcgt aaacgccgtg tgaagaagaa tatagaagca ggtattgcac atatccattc
     9241 tacattcaat aacactatca tcatgatcac tgatactcac ggaaatgcat tagcgtggtc
     9301 atcagctggt tcattaggtt tcaaaggaag caaaaaatca actccttttg ccgctcaaat
     9361 ggcagcagaa actgctacaa aagcagcaat ggaacacgga ttaaagactg ttgaagtaac
     9421 agttaaagga cctggttctg gacgtgaagc agcaattcgt tcattacaag ctacaggttt
     9481 agaagtgact gcaattcgtg acgtgactcc agttcctcat aatggtgccc gccctccaaa
     9541 acgccgtcgt gtttaatgag tgccgctcat tttgagtttt atcagtaacg tcattgaaca
     9601 agacactttt cgttttgaaa ggggtaaaga taagaatgat tgagttcgaa aaaccaagaa
     9661 tcgcaaaaat tgatgaagaa aaagattatg gcaagttcat cgttgaacct cttgaaagag
     9721 ggtatgggac tactttaggg aattccctac gtcgtatttt attatcttct ttgccaggtg
     9781 ctgccatcac taatattcaa atcgatggtg tactgcatga attctctacc atcaaaggtg
     9841 ttagagaaga cgtcactcaa atcatcttga acattaaagg cttagcgctg aaaatgtatg
     9901 gccaagaaga aaaaaccctt gaaatcgata ttaccggacc tgctacagta actgctggcg
     9961 atattatcgt tgatagtgat gtagaaattc ttaacaaaga tatgtacatc tgtagtgtct
    10021 ctgaaggagc tacgttccat gctcgcttaa cagtgaaacc tggacgtggt tatgttcaag
    10081 cagatgaaaa taaaaaagaa gatatgccaa ttggcgttct tccagtcgat tcaatctaca
    10141 cacctgttcg tcgtgtgaac tatcaagttg agaacacacg tgttggacac cgtgaagatt
    10201 ttgataaatt gacgatggag atttggacag atggttcaat tgaaccgttg gaagcaatga
    10261 gtttgtctgc gaaaatcatg actgaacatt tggatatttt tgtgaactta actgatgaag
    10321 caaaaaatgc tgaaatcatg gtcgaaaaag aagaaacaca aaaagaaaaa atgttggaaa
    10381 tgacgatcga agagttagac ttgtctgttc gctcttacaa ttgtttgaaa cgtgcaggaa
    10441 tcaatactgt acaagaactt acaaataaat ctgagcctga aatgatcaaa gtccgtaact
    10501 taggacgtaa atcattagaa gaagttaaag caaaattgca cgacttaggt ttaggtttac
    10561 gtaaagaaga ttaaaacctt ttacgaagga gggaaaccaa cgtgagttat cgtaaactag
    10621 gacgcacatc tagccaacgt aaagcgatgt tgcgtgattt aacaactgat ttattaatca
    10681 acgaacgtat tgtgactact gaagctcgtg cgaaagaagt tcgctcaact gcagaaaaaa
    10741 tgatcacttt aggtaaacgc ggagatttgc atgcacgtcg tcaagctgcg gctttcgtac
    10801 gtaatgaagt tgccagcgtg cgtgaagaaa atgaggacat cgtcattgaa tcagctttac
    10861 aaaaactgtt caacgatatt gcacctcgtt acgcagaacg tcaaggcgga tacacacgca
    10921 ttttgaagac agaaccaaga cgcggtgatg gtgcaccaat ggttgtctta gaacttgtct
    10981 aaaatcacac taataacatc actttataga tgattctttt agagtgttat gatgttggaa
    11041 gatatttttc cgagtctagc tcagcgctgc tcctcaagcg aatatcgctt ggggagtaag
    11101 cactcatcat aaagtgtttt tttatacctt caattacctg tcatgacaga tatgtttgaa
    11161 ggcataaaaa aacattttaa gcgagaaata attgaaaatt ttgtcttttt ttgatagtgt
    11221 tagtaggaac tttaggatga agaaggagta atcatggagc caattattga attagaaaag
    11281 ataaattata aataccagcc agatgatctt cgccccgcat tgaaagatgt ctcctttaca
    11341 attgataaag gtgagtggat cgctattatt ggacacaatg gttcaggaaa atcgacactt
    11401 gccaaaacga tcaatggatt gcttctgcca gaatcaggga tcgtgaaagt gggaaatcaa
    11461 atattggatg aagaaaatat ttggacgatc cgacagatgg ttggaatgat ttttcaaaat
    11521 ccagacaatc agtttgttgg ttcgacagta gaagatgacg ttgcttttgg tcttgaaaat
    11581 caagggattc ccagagaaga aatgctagtg cgtgtgaaag atgcacttga gaaagttaga
    11641 atggctgagt ttgcttcacg cgagcctgct cgtttatcag gagggcaaaa acaacgtgtt
    11701 gccattgccg gcgtggttgc cttacgacct gatatcatta ttttagatga agcaaccagt
    11761 atgctagatc cagaaggtcg tgaagaagtg atctctacga tcaagaagat caaagaagaa
    11821 agtcaattga ccgttatatc tatcactcat gatattgatg aagcagctaa tgccaatcgg
    11881 atactagtga tgagacaagg cgaacttgtc cgtgaaggta caccaaaaga aattttttct
    11941 gctggtcctg aactgatcga cttaggtcta gatctaccat ttcctgaaaa actgaagagc
    12001 gcactgaaag aacgtggggt tgatgtgccc agtgaatata tgacagaaga aaggatggtg
    12061 gattggttat ggacatccgt tttgaacaag tagacttcac ttatcagccg aatacaccat
    12121 ttgaacaaag agccttattt gacatcaata tgacgatcaa agaaaacagt tatactgcat
    12181 tagtagggca taccggaagc gggaaatcaa ccttacttca gcatttgaat gcgcttgtta
    12241 agccaacaag cggtacagtc catattggag aacgagatat tcagccggat acggataata
    12301 agaatttaaa gcccatccga aaaaaagttg gcatcgtctt tcagtttcca gaagcacagc
    12361 tattcgaaga aacggtagca aaagacattg ctttcggtcc taaaaacttt ggtgtcagcg
    12421 aagaagaagc actagtccta gcaaaagaaa cattagaaca agttgggctg gatgaaagct
    12481 atttggaacg ttcgccattt gaactttcag gagggcaaat gcgtagagta gcaatcgctg
    12541 gtgtgcttgc catgaggccg gaagtacttg tattggatga accaacggca ggacttgatc
    12601 cccagggcag aaaagaaatg atggagatgt tttggcgtct tcataaggaa cagcagatca
    12661 ctattgtatt agttacgcat ttaatggatg atgtagccaa ttttgcagat tatgtctatg
    12721 tgttggaaaa gggacgaatc gttaatagcg gggagccgca agaggtcttt caaaacattg
    12781 aatggctaaa agaaaaacaa ctaggcgtac ctactgccac cgagtttgca gaagaattaa
    12841 tggctaaagg gatgaacttt gctaccttgc ctttgacagc tgaagaatta gcagacgcta
    12901 tcgtccatta tgctggagga aatgcccatg atgaataaat tgatatttgg tcgttatatg
    12961 cctggagatt ccttgatcca tcgattggat ccccgagcta aattattagc cagtttttat
    13021 tttatcggaa tcattttttt agcaaataat tgggaaacgt accttttgtt ggcatttttc
    13081 acattacttt ctgtcttctt gtcaaaagta gatttgaaat tttttatccg aggcattcgc
    13141 ccattgatct ggctaatcct cttcactgta gctttgcagg tcttgtttac aagtggcgga
    13201 gaaacttatt ggagttgggg tgttttccat atcactgaat ttggtgtgat gaacggcctc
    13261 tttattttct gtcgattcgt gttgatcatt tttatgtcga ctctactcac gcttactaca
    13321 ccgcctttgg aattatcgga tgcgattgag tacttgcttc gtccattgaa agccgtacgg
    13381 ttcccagtcc acgaaatttc attgatgctg tcgattgctc ttcgttttgt gcctacattg
    13441 atggacgaaa cagaaaagat catgaacgct cagcgcgctc gcggtgttga ttttggtgaa
    13501 ggaaacctag ttcagaaaat gaaagcagtc attcctttgc tgattccttt atttgtcagc
    13561 agcttcaacc gtgcggaaga tcttgcgact gctatggaag caagaggcta caaaggtggg
    13621 gaaggaagaa cgaaatatcg tgtattgcat tggcataata gagacacgat ggttatcatt
    13681 gcttttgtat tattaactgt gctactgatc tttttaagag gataaacgtt cagtgggccg
    13741 ctatctattc ttttcttgct gatctgtttg caagaaagga tgcaagcagt gtatgacaag
    13801 aaatattagg gtctgtggca gactaaggtc acaggcctct ttcttttatc tgaaaagaaa
    13861 gaacgaaaaa attttataga aaaatcatta ttccagataa ccttttagga acaaaggaga
    13921 gaaaaacatg gtacgttaca aagcaatcat tgcgtacgac ggaacaaatt ttaatggatt
    13981 ccaaaaacag ccaaatgggc gtactgttca agaagaagta gaaaaaacat tacaaaaaat
    14041 ggcaaatgga aaagaaatca cggtctttgg ttcgggaagg acagatgccg gagtacatgc
    14101 gatcgggcaa gtaatccatt ttgattatcc agaggagcgt ccgttagaac gaatgcgttt
    14161 cgcgctagat acccagtcac cagaggatat tgctgtaaga caagtagaaa ttgtttcaga
    14221 agacttccac gcaagatatt tggtcaaaga aaagacgtat caatttcgag tggatatagg
    14281 gaaaccacgc agtccttttc gacgccatta tgcaagttat tttccttacc cacttgattt
    14341 ggaaaaaatt cagcgggcgc ttcctgattt attagggact cacgatttca cctctttttg
    14401 tgcatcagga agctcgatag aggataaaat acgtacgatc tacgaggcta agatggaggt
    14461 aaatgaaaca aaagacgaat tgttgtttac atttagggga aatggcttct tgtataagat
    14521 gatacggatc ttagtaggaa cgcttttgaa aattggcaat ggccgattgc ctgaagatag
    14581 tatcccagat attattgcga aaaaagatcg taatgcagca ggacctacag cacaccctga
    14641 aggactttat ttgtatgaag tgaaatatga ataagaggtt atgatagaag tggtcagctt
    14701 caagaaataa gaaggtattt acgaaaatta cttctcaaaa ttttgcaaaa tttcggctta
    14761 ttttcgaaga agctacttct ggaacaccgt ttaagaaaga ggtcgtgagc ctgtcattta
    14821 actctgagtc acaaagagca atttcaaaaa acagcttctc atctttgatg aatatcgtga
    14881 cttgtggacg aggcgttgat aagtgaatca tctttttgtt acctattttt tctgtaacca
    14941 aaaaacgatg gaacgttgtt ttgattgccc ttggtgcgga cagtctgaac aggttccatc
    15001 gtttctttat tttctacgtc ttcttctgtg tgtgtttcgt ggttgttctt ctattgctgg
    15061 atcaatcgtg ccataaaagg ggcgattgcg gtcaaggtac cagacataaa gaataaacaa
    15121 tgcaatacat aggacaaaaa ggattgcacc tatcgtaaat ccttcaggca agtaactgaa
    15181 agtgacagta tgtttacctg ctggaacttt tatcatcaag aatgcgtctt tgaaattgct
    15241 gaccttgact ggtttcccat caattttagc tttccatcct ttgtcataag gaatagttgt
    15301 caccaacatg gaatcttcag cggtatttac agtcccagaa gcttttctgc cagtggcttc
    15361 catttcgaca cctttttctt tgatctgatt cattgcggct tgataggcta caacatctag
    15421 accaacgact ttaggctcca tgaaacttat ttctttcgtc ccataaaaac ttgcagtaaa
    15481 tgtcactgtt gtctcttgag gataatagcc aagatcatag tattgaccag aaatgttgat
    15541 ttgcgttttt cgcgatgtac cattgacagt gatcgtcgca ctagaacttt ctaattgcgc
    15601 aaaattggtt gggaacaaac ttaagtaagc ttgtgtattt gcaggcacat ggacttccca
    15661 agtgagctct tttggctggt tatctgctgt ttctgagtaa gtgacgccag tcgttgtctt
    15721 tgtgatcgtc gtattcaact gattcttcaa agtaggtgta tagaatgtaa aatactgttc
    15781 atttagacca gacaatgtat tgatcaagtt tgtctgactg gtcaaattat ctgtcactgg
    15841 ctgctcaaca tcattaacgg acaacggtgc agtaaatgct agtggcaatg cattgacatt
    15901 ctgatacagt gtatagtcgc cgctttgacc agcttggcgg aagccatatt tactgaacgt
    15961 gctttcgttt ttagaaatgt tatatttgat tcctgtaaac ccatccatca gcaaagtatt
    16021 attgttataa cggatattca aatttgtccc tcttgaacgg aaacctaatt tatccaggta
    16081 agaggaagaa ttacgatttc ttatagaaga aaacaaactg ataccactgt aaccatagtt
    16141 gatcgaatcg tttgcagata ctggatccag attttccaag cggtaaaacg agtcggaatc
    16201 tttttttgtt tcatttacaa gagctttcaa gtcaggataa ggatcagtat ataaactgcg
    16261 ggaagcataa ttccaatcat caagaatgcc atggatcata ctgttcgtat tgaccagtgc
    16321 ttcagcagac atgatcaata ataacaatat actcagatga ttgaatgaaa gttttttatg
    16381 ctgataaaat cccacagcaa aagcataaat ccctaagaaa accactgtca atacaaagga
    16441 agtcatcgtc acataggtgt agcttccttc aggtttgatg ccccatgcca ccgcaaagag
    16501 agcagctacg accaaaatga ttcctaacaa cagcccacca tcgtcttgtt ctatcttttc
    16561 ccatcctttt cctgcaagca ggagcactaa gaaagagaac agataggcat aacggaataa
    16621 aaacatattc ggtgcatgca taccatgcca gaacaaattc aatggaacaa gataaaaact
    16681 tgcgataagt atggcgaaaa gactgccgta caaaaattta tttttcaacg gtactttccg
    16741 actggcaaaa taaaacagac agaggactaa tggtagcaga cctgcgtaaa cgaacggaat
    16801 cgatccgtat tttgtcgtgt cataaacacc aatcatattt ttcatgatta gatccagata
    16861 agaagtcgct tctgttttca acgtagtgat ctgactcagt gcctcgccat ttgaccgaag
    16921 atccagcact gctggcaata caatgatcat tgaagcgccg cctgccagta aagaagtgat
    16981 accataagga acaatcgttt ttttatattc tgaccagttt gtcaatagac gggcgataaa
    17041 atataagaaa gaaaatacgc caatcataaa acccatataa aaactggata aaaacaagag
    17101 taaataactg ataaataaaa gtttcggccg tttaagatcc ataacacgat gaatccccaa
    17161 aatcactaat ggaagataaa caaatgcatc cagccacatg atgatctccg aatgtgcagt
    17221 gataaatgag atcaatgcgt aactgacgct aagtgcaaca tgcccccagc gaggaatctt
    17281 gaacgtacgt gcaagaaacc aaaaagccaa tccggcacag ccgaccttaa gcaaagtcag
    17341 aacatacagt gcatctggca tcatctggtt tggaaaaaag aacaccagtg gcgtgaagat
    17401 cccgcctaga tagtaagaaa ttaaagataa ataattcaat cctagagaag cattccatgt
    17461 ataaaaaata ctttgctttc caagaagaac atttcggaag ctggcatgaa aattagaaaa
    17521 ctgtgaaaag gcatcacttg ccaaaatact tctgctgctt ccaggataga ttccgatact
    17581 tagataaaca agtgctaaaa tcaaaaacgg aatcaggaaa ctagctgctg cgtagcgtcc
    17641 gttttctcta aagaatgact ttaaattttt tttcatattt attttccacc aaatttctat
    17701 gaagttgtat tcagtttatc atatccttgt ttttctctgc ttaaaaagag tctagttttc
    17761 acaaaaaaat aaaatttaga ataaataact tcataatttg ttcgtataac aaaaataaac
    17821 gcaaaaaaag ggtaggaatc aaaagtccca cccttaaaaa tttttattaa ataaaaaacc
    17881 gttcactatc tgaacggtcc aagtatcatt caattaaacg cgttcaactt ttcctgattt
    17941 caaagcacga gttgacaccc aaactttttt aggttttccg tcgaccataa cgcgaacttt
    18001 ttgtaagtta ggtttaacag tacgttttgt tgagttcata gcgtgtgaac ggttgtttcc
    18061 actttttgtc ttgcgacctg taaagtaaca aacttttgcc atggtaattt cctcctttgc
    18121 cttgatttgg agcaatagaa taattcagct cataccagac taacatatca tatagaatgg
    18181 gaatttgcaa gtacattttc atagatacaa ttgacaattg agaagaataa ttgtaaatta
    18241 aacaaggtta tacctgaaat tttgcaaggt ttttgagtac tttgggtaac tgccttttat
    18301 ttcaagttgc gctatgataa aatatgatag aagaaaaatg ggagtttttt acccgttaag
    18361 gaggctcatt catggctgta aaaattcaaa cgtcagctgg aaccattgaa attaccaatg
    18421 atgtaattgc aacagttgtt ggtggcgccg cgacagatgt gttcggtgtt gtaggaatgg
    18481 ccagcaaaaa tcaaattaaa gataatatta ctgagatttt gcgtaaagaa aattactcaa
    18541 gaggagtagt tgtccgccaa gaagataatg gtattgctgt agatgtttat acgattatca
    18601 gctatggtac gaaaatctct gaagtttccc gcaacgtcca agaaaaagtg aaatacaatc
    18661 tagaggcatt attaggcgta actgccaatt cagttaatgt ctttgtacaa ggtgttcgtg
    18721 tactgccaga ctaggcagga gactggctgg taactcagca gtcgatctga aggaggatta
    18781 tctaggtgag tgtaacagaa ataagtgcaa gccagttcca ggaaatggtc caggcaggtg
    18841 cgaatcgtct gcaagtaaat gcagaatacg tcaattcatt aaatgtattc cctgtgccag
    18901 atggcgatac gggaacaaat atgaatctat ctatgactag tggagctaag gcagtagtag
    18961 attcaacctc agaaaaagta ggagaattag cagcaatgct ttcaaaagga ttgctgatgg
    19021 gtgcgcgcgg aaattccggc gttattttgt ctcaattgtt tcgtggattt tctaagcaaa
    19081 ttccagatgt tgtctcatta aatgcttctg atcttgctgc agccttcact catggagttg
    19141 aaactgcgta taaagcagta atgaaaccag tagaaggaac gattttgaca gtagctagag
    19201 aagcagcgaa agctggagaa aagaaagcaa aaagtactga tgatgtcgtt gaagtaatga
    19261 cagctgttgt aaaaggagca aagcgtgctt tagctaaaac tccagacttg cttccagtcc
    19321 taaaagaagt cggtgtcgta gacagtggag ggcaaggact gctgtttgtt tatgagggct
    19381 ttttaagtgc attgaatgga gaattccaag cggatgaaac atatgaacct tctccagcag
    19441 aaatggatga tatggtcaat gcagaacatc atcgcagtgt ccaaggtcaa atggcgacag
    19501 aagatatcca ttttggttac tgtacagaaa tcatggtaaa aatcggagaa ggacctacag
    19561 tcgatagtac attcgattac gaagaattcc gtaactattt agatggtata ggagattctt
    19621 tacttgtggt caatgatgac gagatcatca aagtccacgt gcatacagaa aacccaggag
    19681 aagtcttgaa ttatggacaa aaattcggtt cattagtcaa aatcaaagtc gacaacatgc
    19741 gcttgcagca tgagacgatt ttggaacacg atgaagaagt gaacgagttt gcgaaaaaag
    19801 aaataccacg tatcccgtat ggtatcgtag cgatcgctgc tggtaaaggc gtccaagagc
    19861 tgtttgaaag tttaggcgct aattatgtta tcagtggtgg acaaacaatg aatccaagta
    19921 cggaagacat tgttaaagcg attcaagaag taaacgcaga taaagtgatt gtcttgccaa
    19981 acaataagaa catcttcatg gcagctgatc aagcagcaga agtagcagat atcccagtag
    20041 cagttgttcc ttcaagaacg atttcccaag gattgacagc tatgctttca ttcaatgatc
    20101 aagcttcttt agaagaaaac aaagaaacga tgacagaaat gttgtctagc gttgtgagcg
    20161 gtcaagtcac tcacgcgatc agagatacct caattgacgg tgtagagatc aaagaaggag
    20221 actttttggg catgatcgat ggaaaaatcc tgatctctaa cccagatatc acagcaacag
    20281 cgttaagtac actagaacaa atgatcaatg aggacacaga gatcgtaacg atcttggcag
    20341 gtgatgaagg aacagttgaa caagcaaatg aatttgctga gaaactaaca gaaaaacatc
    20401 ctgatttgga aatcgagatc caccaaggag atcagcctgt ctatccttat ctattttctg
    20461 ctgaataatt tctgtgtcag aatatctttc taacacagta ttcattcata aaaggccttc
    20521 ggttagtgat caggtatgat cgctgaccaa gtcctttttt tgttaaagga gggaggaaaa
    20581 atttatgcgt tctttagatg atccaatcac tatgctggca ggagtcggag aaaaaagagc
    20641 agctagtctt gccagcttgg gcatccaaac gattgaagac ttactgactt attatccttt
    20701 tcgctacgaa gatattcagg aaagaaatct aaacgagatc caggatcaag aaaaggttac
    20761 tttgaaagga atcgtagtct cgcctcctgt gatgaatcga tttggttata aaaaaagccg
    20821 attgcaattt cgtatgatgc aagaacatga tgtgttcaac gtttcttttt ttaatcaacc
    20881 ttatctaaaa gaaaaagtca ttttatcgga ggaaatcgcc gtatacggta aatgggatgc
    20941 aaagcgtaaa gccttgaacg ggatgaagat tttaggttct caaacaatgg atgacttttc
    21001 gccgatatat cacgtcaaca aatcaattcg tcaaacgaca ttagttgatc taatcaaaaa
    21061 agggtttcaa gaatacggca cattgattga agaaaatctg ccaaacaacc ttgtggagaa
    21121 atatcgactg cttgaccgag cttctgctgt aagaagtatg cattttccaa aagaccacga
    21181 agaaaaccat caagccaaac gacgtgtcgt ttttgaagag ttctttttat ttcaaatgcg
    21241 tcttcaagga ttgaaaaaag cagaaaaagc agaaacgaat ggtttggaga tcctttatga
    21301 tgtccaaaaa ttgaaacaat ttactcaaaa gctacctttt gaactgacaa atgcacaaaa
    21361 acgtgtaacg aatgaaatct gtcgggatct tcgcagtccg cagcatatgc agcgactttt
    21421 gcagggagat gtaggtagcg gaaaaacagt ggttgccgcg attgcgttat acgctaccgt
    21481 tacagcaggt ttccaaggtg cgttgatggt gcctacagaa atcttggcac aacaacatat
    21541 ggaaagcttg caacagctgt ttgatcccaa tgaagtaacc atcgcattga ttacggggtc
    21601 aacgaaagca aaagaacgac gtgagctcct tgaaaaacta gagcaagggg aaattaacgt
    21661 cgtgatcggc acacatgcat tgatccaaga tggagtcgaa tttcagaatt taggtctagt
    21721 tattacggat gagcagcacc gttttggcgt gaaccagcgg aaagtacttc gtgaaaaagg
    21781 ctggcgacca gatgtcttat ttatgacagc gactccgatt cctagaacat tagctattac
    21841 agcatatggt gagatggatg tatctatcat tgatgaactt ccagctggac gtatcccgat
    21901 cgaaacacgc tgggtaagaa cacctcaatt ggacagtgta ctggattgga cgaaaaaaga
    21961 actggcaaga ggacatcaaa tgtatgtca
//
LOCUS       DACEXQ010000021        21516 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.021, whole genome shotgun sequence.
ACCESSION   DACEXQ010000021 DACEXQ010000000
VERSION     DACEXQ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.021"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..1154)
                     /locus_tag="IXH04_000650"
     CDS             complement(<1..1154)
                     /locus_tag="IXH04_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAQ1851282.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYP"
     gene            1284..2159
                     /locus_tag="IXH04_000651"
     CDS             1284..2159
                     /locus_tag="IXH04_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAQ1851283.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            2239..3213
                     /locus_tag="IXH04_000652"
     CDS             2239..3213
                     /locus_tag="IXH04_000652"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ1851284.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            3402..4538
                     /locus_tag="IXH04_000653"
     CDS             3402..4538
                     /locus_tag="IXH04_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ1851285.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            4675..5019
                     /locus_tag="IXH04_000654"
     CDS             4675..5019
                     /locus_tag="IXH04_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAQ1851286.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            5290..6576
                     /gene="efmA"
                     /locus_tag="IXH04_000655"
     CDS             5290..6576
                     /gene="efmA"
                     /locus_tag="IXH04_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAQ1851287.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(6714..7313)
                     /locus_tag="IXH04_000656"
     CDS             complement(6714..7313)
                     /locus_tag="IXH04_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAQ1851288.1"
                     /translation="MNYFISDMHFFHEHLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            7717..8838
                     /gene="mnmA"
                     /locus_tag="IXH04_000657"
     CDS             7717..8838
                     /gene="mnmA"
                     /locus_tag="IXH04_000657"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAQ1851289.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            9084..10505
                     /gene="arcD"
                     /locus_tag="IXH04_000658"
     CDS             9084..10505
                     /gene="arcD"
                     /locus_tag="IXH04_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAQ1851290.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            10519..11637
                     /locus_tag="IXH04_000659"
     CDS             10519..11637
                     /locus_tag="IXH04_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAQ1851291.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(11697..12989)
                     /locus_tag="IXH04_000660"
     CDS             complement(11697..12989)
                     /locus_tag="IXH04_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ1851292.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            13139..13699
                     /locus_tag="IXH04_000661"
     CDS             13139..13699
                     /locus_tag="IXH04_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1851293.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            13828..14205
                     /locus_tag="IXH04_000662"
     CDS             13828..14205
                     /locus_tag="IXH04_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAQ1851294.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            14138..16210
                     /locus_tag="IXH04_000663"
     CDS             14138..16210
                     /locus_tag="IXH04_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ1851295.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEEQKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKEMRINPDF
                     TIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKMLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(16421..17368)
                     /locus_tag="IXH04_000664"
     CDS             complement(16421..17368)
                     /locus_tag="IXH04_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1851296.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDVDSVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            17613..18077
                     /locus_tag="IXH04_000665"
     CDS             17613..18077
                     /locus_tag="IXH04_000665"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAQ1851297.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQQILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(18139..19182)
                     /locus_tag="IXH04_000666"
     CDS             complement(18139..19182)
                     /locus_tag="IXH04_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAQ1851298.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGIKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(19682..20647)
                     /locus_tag="IXH04_000667"
     CDS             complement(19682..20647)
                     /locus_tag="IXH04_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAQ1851299.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            20881..>21516
                     /locus_tag="IXH04_000668"
     CDS             20881..>21516
                     /locus_tag="IXH04_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1851300.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YPLPQLMKHLIKRFPKVHFEIEIANSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVA"
BASE COUNT         7033 a         3890 c         4490 g         6103 t
ORIGIN      
        1 gggtagccta cttggatacc taagttattt aattttatga cggctttttc ttttgttgca
       61 tccccaagcc agtcattttc ttctagacgt tttttataaa cggcgatcat ttttttcacc
      121 atttgttcta cgtctttttt agcagcttcg ccaaaatatt tttttccgta gtaatcgcca
      181 actacttgat cgaattgtcc agaggcaagg taatacgctg cttttttctg aggcattgct
      241 tcgtctgttc cagacaatac gcgagaatag attccgctga cttggcggaa ttcttctgat
      301 agataaccac taagagaacg tactgtttta atgatcatcc agcttttgaa taacggaaaa
      361 tgagcttctg tcaggatgtc attcaaatga tcgaagtaca ctggatcagt tacgatcact
      421 ttatctggag ttgtcccaat caaacccgtg acaagttttt tcaagtccag tttatctgtg
      481 tatttaataa atgtatcaaa agattgcgga ttatacattt tgctgtaatc tgcgttttct
      541 tctgcacttt ttacatgagg agcaatcaat ttatcaaatt gcactgcttg ttccgtgatt
      601 gcttctgcct gacttttttc tttgccggca agctctaata actgaacagc catatcaaag
      661 aaaacgccta ttagttgtgc accgttggga tggtctgatg cataatacgt cttatctgga
      721 aggaataaag aaggcggata agcaaagaag gcgttcgttt gcgcattctt catatctgca
      781 tctacatcca gattaaatgg caatggtaat gtatccaaca gccattctgg aaattgctta
      841 ttcaattcgt cgaaagactg caatgattca actttttcga ttataggttg aagcggttga
      901 gcatgcaact catttcttcg gtcataatcg tttgccagtt gataatatgc aaggaaatgt
      961 ttcatttttt ctgaagacac tttttctgga tgggcaagca gatcgtctgt ttctcccatc
     1021 aaaagttggt cgatcccgtc aactaaatct tggaaaccgc ctgtagctgg tttatctgca
     1081 gggatttttg ctgttttttg ccattcttca ttgactgctt catagaaatc ttgtttcaat
     1141 cgttctttgt tcattcatcg atcgctcctt ttttgtttcg tgtgcccatt atcttacaaa
     1201 atgaaggaat taacaaatag aacataaaaa attgaaaatt cgaacaatca aactgatttg
     1261 tttttttcaa tccttcactt ataatgaaaa aaaggaggcg atcccaactg aatcaagtag
     1321 tggataaacc tttatatttc aaagtagata aaaaaattaa aaaattagcc agcactcagc
     1381 agctgcagtc aagaaaaagt aaattgcttt ttttagctct ggtttttgaa gatcaaagtt
     1441 atgtgattat cgatcaatca ggacatccgg tagaatattc tccagcaaaa tatacttatc
     1501 aagagggtct ttcttgtaaa aaatggaagc tgatcaatga agagccaatc gagttatcta
     1561 gatggatcaa cagaaaagaa gatattcccg ttttgatcga agaaaaaagg tcaggaaagg
     1621 aactcgcaaa ttgctgggtg ggattgccag aagaacgttt tttgaggtat aagaaatggg
     1681 ctaccccctc aggctatctt tgtggtactt acgcggcagc agttctcctt gcctattatc
     1741 aagactatcg taaggggtgg atgcttccaa atgagattcg caagaaaaat acggctgatt
     1801 cgttggtttt gacgaaggca ttgcgtagcc agatccaacc actaggactt cctacgattc
     1861 cttttcaggt tagtacaggg atttctagtt ttttaaagaa gaatggcaat catgagcgtg
     1921 cacgagcaac tcttctaggt tcatggcagc gagctacaaa acgtatacgt gaaggcaaac
     1981 ctgtgatgat tggtattttg aaagtacttg gcagtacata tggcaatcat tgggtgacag
     2041 cgtacgctta ttttgaaact gaaacaggag aacgttatta caaagtacac gacaactggg
     2101 gcgattatca taaagtcatt cctgccagtt ggagtaacgg aacagtttca cttccttaag
     2161 aatttgctga aattaacaga aaatcttgga aaaatatgtt aaactatcga agaatagaga
     2221 agttttgggg aggactacat gagtaataag tatcaggatg acacgttgaa gatttttagt
     2281 ttgaatggca accgtccatt agctgaaaaa atcgcgaaag tgttcggaac agaattaggt
     2341 aaaagcgtcg tgaagcaatt tagtgatggc gagatttcca tcaatatcga agaaagtatt
     2401 cgaggggatc atgtgtatat cgttcaatca acgaatgcac ctgtcaatga ttactatatg
     2461 gaactactga tcatgatcga tgcaatgaag cgagcaagtg cgaaaacgat caatgtcgta
     2521 ttgccttact atgggtatgc taggcaagac cgcacggcga aacctcatga accgatcact
     2581 gcgaaattga tcgctaactt gatcgaagaa gcaggggcaa ctcgagtact gactttagat
     2641 ctgcacacgg ttcaagtaca aggattcttt gatattcctg tagataattt atttacgatg
     2701 ccgttgtttg ctcattatta ccgtcaatta ggtctgacgg gagatgacat cgtagttgtt
     2761 tcgccaaaaa acagcggtgt tcagcgtgca cgtagtcttt cagaatattt gaacagcaca
     2821 ttagccatcg tagatcatgc agatgatgct tcaactgagg gtggttatgt gatcggtgat
     2881 gttcaaggaa agacatgtat catggtagat gatatcttga acacaggagc aacgcttgca
     2941 cgagcagcaa atgtattaaa agaaaacggc gcaaaagaag tatatgcctg tgcgtcacat
     3001 ggattattgt ccgatcatgc gaaaagtatt ttagacaatg caccaatcaa agatatttgt
     3061 atcactgatt ctgttttcac agaagaaaat cgtcatccag aaaacttaac aatcgttaca
     3121 tgttcagaat taatgggtga agcggtcaaa cgcatccatg aaaatacacc gatgagtccg
     3181 ttattccgtc tggaagaaaa agaattcgaa taaacaaaaa aaagagggaa agaagttgtg
     3241 acagaagtga gcagcttcaa ggagctgcga caagactttt gtcacagctc cttttttcta
     3301 tgcccatgaa acgtcctcta gacaaaaatg agctcttctt tggtttgttc gctattttat
     3361 gctagaatgt gcctagcaat aatttggaag ggatgaatga agtgaaaact atttatctgg
     3421 atcatgcggc aactaccccc atgcatccac aagtaattga agcgatgacg gcaatcatgc
     3481 aagacacgtt tggaaatcct tcaagcatac atggatttgg tcgccacgcg catgaaaaat
     3541 tggaaacagc aagacaaatc attgcagaca gtctgcaagc aaaacctcac gagatcatct
     3601 ttaacagtgg gggaacagaa ggggataata cagctatctt agaaacagcg ttctcgcgtc
     3661 aaaaagaagg cagacatcta atcactacag ctatcgaaca tcctgctgtt cttaatacca
     3721 tgaaatattt agagacaaag gggtttgacg ttacatatct gcctgtagat gaaaaaggga
     3781 atctgtcgat cgaagaggtc aaaaaagcgt tacgagaaga tacgatcttg gtctctgtga
     3841 tgtacgggaa caatgaaatc ggaaatttga tgccaatcaa agaaatcggt gaacttttag
     3901 cggatcatcc agcttatttc catacagatg cggtacaagc gtacggaaac caaacgatcc
     3961 atccagaaga attaggaatc gatttattaa gtatctctgc tcataaaatc aatgggccaa
     4021 aaggcgtcgg tttcttatat aaacgagacg gagtaaatat ccctgcattg cttttaggcg
     4081 gggagcagga agaaaagcgg cgtgcaggta cagaaaattt agcagggatc tggggtatgg
     4141 caaaagcagt tgaactgctg tcacctgaag aacgtgaagc acgtaacaac aaatataaag
     4201 gattccagca attgattctt gatacattag aagcaaacga aatcccttat caagtaaatg
     4261 gtgatttaac gaataaactc cctcatgtgt tgaatcttcg cttaattggc gaagcaaatg
     4321 atttgctact gatgaagctt gatcttaatg gggtcgctat ttctactggt tcagcttgta
     4381 cagcaggaaa tatcgagcct tcacacgtat tagaagcaat gtatggagaa gatacaccga
     4441 tactaaaaga atccatccgt atcagctttg gtttgggaaa taccgaagac gacatccagt
     4501 atttccttga tcaattagtt cgtgctgtca aaaaataact agtgaagtca ttcttatatg
     4561 aacagatgtc tgagagaacg ccttcatttt ttagggaact taactgaaaa acctagtaaa
     4621 aatcaggata aacttttata ataaagagaa actaatttta tcgaggtgaa aatcatggca
     4681 tttgaaacaa cagcaacggt actgggcgca ccaattagct atcgtttgca tcctgatgca
     4741 aaaagatata cattgcgcga taatggattt acagaaacaa acggaggcaa ttatcaattg
     4801 atccgaccat tagatgccac gccgcaaagc aaagaaggat tcaaattaaa aatcactgta
     4861 tcaaaagata ttcaaacatt gaaaatgtcg atcactacag caaatggatt gaaagcagtc
     4921 aatattttca aagatccaaa acagaaaatg agccaagata aattttattt tctgatggat
     4981 gggatgatta gccgtgggtt attcgaaaaa gtcgaatgat ctgtggatat aaaaagacaa
     5041 ctagttttga aacggcctat tgttagatga ctaactttgt aaggacgacg caaattagtt
     5101 gtctttttta tttttaaatt taacacatgt tgttattttt aatattttgc tgatttattg
     5161 tttctaagta aagaaattta aattgaaacg attgttattt agggaaaaag gtattttttg
     5221 aaaaaagaat cccgtataat gattgttgtg caaacgctat acaaaatgaa aaagaaaaga
     5281 gggatgttta tggaaaatga acagtcggtc gttttaacga attggaagag aaattatttg
     5341 ttctttttat cgggacagtt tttgtcagga atcactagta tggtcgtcca atatgcgatc
     5401 atctggtatt tgacaagaga aaccggttcg gcgacaattt tgagttttgc tactttacta
     5461 ggaatgattc ccatggtttt actaagccca tttgtaggac cgctagtgga tcgctgggat
     5521 aaaaaagctc ttttgattgt cacggatatt attgtagcga ttttcgcgct tattttagca
     5581 gtagtcggaa cgatttcaga atccttcccg atttggctag tttttgtgtc tttgtttatg
     5641 cgttcggtcg cacaaacatt ccaaatgcca acgatccagt cgattatgcc gacaatcgtt
     5701 ccatcttctc acataacaag gacgaatgga cagttaggaa tggtccaatc agcgaatttc
     5761 atcattgcgc cagctctagg tgcggctttg ttttcagtcg ttcctgtcaa ttaccttatt
     5821 ttattagatg tattgggggc agtttttggt gtcggcttat tgatttttgt gaagatccca
     5881 aaagtttctc ctgaaatatt ggaagttccg cttactattt ttaaagatgc aaaatttggg
     5941 ctgcagcaat tgatggataa taaagggtta tggtacataa cgatcaatgg tgcatttgtg
     6001 atgctgttgt tcatgccagc aatcagtctg tatccattga tgacgctaga ttactttggt
     6061 ggttccgttg gacaggcggg agcagtggaa gtagtctacg cagtcggaat gctcttaggc
     6121 ggtgcactga tcagttttat aggaacatgg aaagaccgaa tgaaacctat tattatagcc
     6181 tacatcatta tgggcctgac gatcggtgca agcggattgg ttccaaacga tagtcaaggg
     6241 ttcttgtact ttcttatctt gaatgcagga gcaggttgtg caacgccata tttcaataca
     6301 ctgctgatgg caatgatcca gcagagttat gaatcgaatg ttttaggtcg tgtcttaggg
     6361 aacttcaact ctttgatgaa cctggcaggt ccaattggat tattatttgc aggaccatta
     6421 gctgatcgtt taggggtaga aaaaatgttc ttgttttccg gaatcgggat tttattatgc
     6481 ggaatcgttt tatttttaac gtcagctgcc cgaaattatg ataaagaatt acagaaaaaa
     6541 ctggtaaaag aacaccatga gcaaaaagac gaatagtatg gaatggagtt gtgacagaag
     6601 tcttgttaca actccatttc tgatcctaat gttcctgtgg caataagcct actcaaatga
     6661 aaaatatgag taattatctt tatttgttta atacttgata tagggcagtt ttttcacgac
     6721 ctatcgtact tcctctttgc ttcatagatt tctttgattt gttgctcaga taacggagtg
     6781 ccaaatggta gtttttcctt taagagttct ctttccggtg cgtctacgcc tacattgaca
     6841 tcgttctcga taggtacgct gtaatgatgg atatgtccat gaagattgcg gatagtgtcg
     6901 ttaggcccga gcaacatcgg ataatgagtc aagtaatatt gttgatggtc gaatttaacg
     6961 atggcaccta catcataaaa ataaaatttg ggacttccgt cagtaagcct aggatcattt
     7021 ttttctaaat aacggaaaaa agcgcgactg tcatggttcc ctttgatcaa gtggattgtt
     7081 ccttgcagtt gagtgaccag ttccaaaatt tctgcatttg ctttttcata ttgcggatgc
     7141 atagcaaggt cgcctagatg ataaacatgg tcggtttctt ttattactgc attccagcta
     7201 gtgatcaact gttggttcat ttcatccaca tttgcaaaga gccgcggagc aaagtcgttt
     7261 tttcctaata agtgttcatg gaaaaagtgc atatcactga taaagtaatt catgtatctt
     7321 ttgcctccct tttttcttag agtaaacaaa atcacttatc tgagcaactc ataacatcta
     7381 aaaagaaaag gttgtaaatg agacgtgcag ctttaagcga taagaagcaa ttttgaaaaa
     7441 tagtttttca caatttataa aattattcct tattgccgaa aggctggccc tttttatgag
     7501 tatgccaatt ttttatatcg aatgctcaaa agatgagtga ttttttggtt gactatcatg
     7561 tttgtgtact gaataggtcc agtagaacag tatttttacg acttgataat taagggatga
     7621 aattttcaga aatatgacgt ataatgtaaa atactgaaaa gttgcgacct tcaactcgta
     7681 gatgtttcag aatctaattg aaatgatggt gatatcatga cagacaacag caaaacacgc
     7741 gttgtcgtag ggatgagcgg cggtgtcgac tcatctgtaa cagcactttt gttaaaggaa
     7801 caaggatacg acgtgatcgg aatattcatg aaaaattggg atgacacgga cgaaaacggt
     7861 gtatgcacgg ccacggaaga ttataaggat gtagcgaaag tagctgctca aatcgggatt
     7921 ccttattact ctgtgaattt tgaaaaagaa tattgggatc gtgtatttga atatttccta
     7981 gccgaatacc gcgcaggacg tacacctaac ccagacgtga tgtgcaacaa agaaatcaaa
     8041 ttcaaagcat tcttggatta tgcaatggat cttggcgccg attatgtagc tactggacat
     8101 tatgcgcaag tcactcgcga tgaaaacggc gtggtccaca tgcttcgtgg agtagacaat
     8161 aataaagatc aaacttactt ccttagccaa ttatcacaag aacagctttc aaaaacgatg
     8221 ttccctctag gcggcatgga aaaatcagaa gtacgggcaa ttgctgaacg cgcaggcctt
     8281 gcgactgcta agaaaaaaga ttcaacggga atctgcttta ttggtgaaaa gaacttcaaa
     8341 caatttttga gcaactatct gccagcaaaa aaaggaaata tggtcactct tgatggtgaa
     8401 gtcaaaggac aacatgctgg cttgatgtat tatacgattg gtcaacgtca aggactaggg
     8461 atcggtggcg gaggagattc gcaagaaccc tggtttgttg taggaaaaga tctagcgaca
     8521 aacacattat atgtggggca agggttccat catcctgcat tgtatgccac aagcttggat
     8581 gcaagtgaaa tccactttac gacgaatgaa ccaatgccaa aagaattcaa gtgtacagcg
     8641 aaattccgtt atcgtcagca agacgttcct gtgacagttc gcttattgga tgataatcgt
     8701 gcagaagtcg tattcgacga acctgtacgt gcaatcactc ctggacaagc agttgtattt
     8761 tatgatggaa tggaatgtct gggtggtggt ttgatcgacc acgcatatca agaaacaaaa
     8821 gttttacaat acgtttaagc gatattaaat gaatcaaaac agctatggct aacaaaagaa
     8881 acaccttttc ttttggatag catagctgtt tttttgcaat aaaattaatt tttattcatt
     8941 ttatacttga aatacttgtg taaaaagttc ataatatcca ataatatgaa taaagttgct
     9001 ttttgttaag caagagcagt tgcaaaatag aagggaatct tttgcaagtg atttgttcaa
     9061 ttttataagt gagggtgatg gaaatggaaa agaaatcgac gggtattagc aaatggggac
     9121 tggttgctct ggttgtcagt tcgtcaatcg gaagcggtgt gtttgggatc acaagtgatc
     9181 tggctagttc tgcagctcca gggccagcga tcctttcatg gataatcgtt gggattggta
     9241 ttttagcttt ggtattatcg ctgaatcatt taggagaaaa gcgtccagat ctagatggtg
     9301 gtattttcgg ttacgcggaa gcttcatttg gtaaattagg tggcttcatc agtggttggg
     9361 gttactggtt gtctgcgtgg ctgggaaatg ttgcttttgc aacgatgctg atgagcgcca
     9421 tgggagaatt tttcccgata ttcaaaggtg gacaaaatgt tccttctatt ttatttgcaa
     9481 gtatttttat ttggggtctg actttattag taaacaatgg agttgaagga gcgagcgtca
     9541 tcaatacaat cgtgacgatc tgtaaattag tgccattatt cctattttta atatttgtat
     9601 ttatcgcatt taaagtgaat atattcactg ctgatttttg gggtaacgta tctgataatc
     9661 tagtgaatgg aacagggcag caaggaacga tctggttgca gattaaaggt tgtttgatgg
     9721 ttatgatgtg ggtctttgta gggatcgaag gcgcaagtgt tttagccaac cgcgcgaaga
     9781 aacgttcaga agcccagcaa gcatcgatca ttggtctgct ttgtttattg tttatctata
     9841 tccttgcttc gatcttgcca tacggcgtct tatctcaaga agagttagca actatttcac
     9901 aacctgctat ggcaaatatt ttaaaaggaa tcgtgggaaa ttggggagca gcgctgatca
     9961 atatcggctt gattatttcg atcattggtt cttggttgtc ttggacgatg ctcccagcag
    10021 aaacaacgat gttgatggct cgagacggtc tgttgccgaa aggttgggga aaaacgaata
    10081 aaaaaaatgc cccaacttat tcgttagttg ttactgcaat cttgactaat ctattcttat
    10141 tgactttctt agttactgat tatgcctatc aatttgccta ttctttatgt acagcggcaa
    10201 ttttgatttg ttatctactt gtcgggatct atcaagtcca gttttcttta aaagaaggaa
    10261 tcaaaaaaca agtagtgatc ggtgggattg ctgtagtttt tgaattgatg ggcatcattt
    10321 tagcaggatt ttcttatgtc cttttatgta gtattgccta tcttcctggt ttttgcttct
    10381 actggatcgc ttgtcgtgaa accaaacatc aaattacggg aaaagaaaaa gtaatgattg
    10441 ggctgatttg tgtggccgca ctgctttcaa tcgggttgct tgctgtaggc tggatcaaga
    10501 tataaggggg aaagaaacat gaaaatcgca aatttcggtg tagaagaatg gctaaataaa
    10561 tgggaaaaga aagcggtata tgatattgca caaagctcga tcgaagcatt gacattagac
    10621 gaactcgttg gcttagatgg gactagcgta agtaaatttt tcgaaaaacg caaaaacgaa
    10681 ccattggact atggctggat cgaaggatca gatgttttca aacaagaagt cgcttctctt
    10741 tatcaaaccg tcgatccgga aaacatctta caaacgaatg gagcgactgg tgctaatctt
    10801 ttagctctat acgcgttagt ggaagcagga gaccatgttg tttcgatgct tcctacttat
    10861 cagcaattat atgacatccc caaatcttta ggtgccgctg ttgactttgt tcatttgaag
    10921 gaagaagaag attggcagtt tgatttagaa cagctagaag ccttagtcaa acctgaaaca
    10981 aaaatcatct gtttgaatag tgcaaacaat cctacaggca cattgctaga tcggaaaact
    11041 ttagaaaaaa tcgcagaaat tgctcgtaca gtagatgcat atgtattgat agatgaagta
    11101 tatgcgcctt taacggataa aggtgaattc ttgtcgatcg tagatgtcta tgaaaaaggt
    11161 atagcaacca attccctatc caaaacctac tccattccag gtattcgtat tggctggaca
    11221 gctactggac ctgagttggc agaagttttt cgaaagtatc gagactacac gatgatttgc
    11281 ggcggagtcc tgtcagatga tcttgctgta catgcattga agaataaaga aaaaatattg
    11341 gcgcgaaaca aaaagatcat tacagagaac cttgtgattt taaaacaatg ggtagccaat
    11401 gaaccgaaag ttgaacttgt cgctcccaat tacgtctcta cttctttcat taaattagcc
    11461 atcgaagaag atgaccagac attttgtatc aatttattag aagaaacagg agtactatta
    11521 gtaccaggaa gtgcctttga tttaccgaag catgcccgac taggttactg ttgtaaaaaa
    11581 gaaaccttgg aaaagggatt attcctctta tctgagttct tgaaaaaaca ggcctaaaca
    11641 aaaacactgc aaggacagtc tgtgacaact gttcaagcag tgtttttcat ttacgcttat
    11701 tgtctatgag aaggagagtg ccctgaagct atggagactc ctgaagcttt ttcttctgca
    11761 gtttcttgct gatggatctc ttgtgtattg tgtcgtacaa gcaagaagtt gaccaaaaca
    11821 agaagagaag tcgaaagaaa gacgccacga taaccaaatg cactggaaac actggagccg
    11881 atcataggcc ctactacgtt accagttgct tggaaagatt ggttataact gaagatccgt
    11941 ccagcagctt gatgaggcga atattttgtg atcaatgctt ggacagcagg gagcagacaa
    12001 gcatcagaga taccaatcaa aaaacgtaag gcagctaatt gccaaacatt cgtgacgaat
    12061 gccattggga tataaaccag aatcgcaaaa cccaaaccaa tcgcaagtat tctttcactc
    12121 ccaatcttgt cacctaagcg accaaatctt ggagcggcta tcaaagtggc aatcccaggt
    12181 atcgaagcaa tgattccgct gatcaacgta acattcccat gtccatgcag caattggcga
    12241 atgtacagac tgatgattgg actaatggaa ttgttggatg cttggatgat catcgttgtg
    12301 acgaacatac cgatgacgac gtgtggatat ttcaagtctt tgaaaatctg tttggcagaa
    12361 accatgtttt cttttttcac tgggacaaat ttttcatgga cgaaaaacag actcaacaag
    12421 aagacgaaaa ataagatgat ccctgtaatg aaaaaggtag ggcgatagcc aaacgctgaa
    12481 gcagagatcc cgccaagaag cggtccaagt aatgttcctg taacagaacc agttgctaag
    12541 gttccaagaa cttgaccact tttttctttt ggcgtcccag ttgcgacaag ggcagtggca
    12601 ttactgatat aaccagaaaa aatcccttga agcagacgca atgcgactaa ttgatacaca
    12661 ctagtcacgc aacccatcaa agaaatgacg attgccattc ctaatgaagc acgcaacagc
    12721 atcaattttc ttcctttttg gtcggctaat tttccccacc aaggagagat aagcgtagtc
    12781 accaagaaag tagatgaaaa agtcaatccg ctccaaaagt tcaattgtga cgtactataa
    12841 ttacctaacg tatctatgta aagtgacata aaaggcatga ctaaactaaa tccgatacca
    12901 gccataaatg ttccaaacca taagacaaac aagttttgtt cccaagtttc tcttttccta
    12961 aataattttt cctttattct tgcagccaat tagactcctt ctttctgaaa attttcatct
    13021 acagctcttt attataaact acaatatgta gtttatcaat gaaagtctct tttttctctt
    13081 tctttttcgt ggtaaactga aagagtgaag gatttatttt atgagaggaa acatggaaat
    13141 gaaaaaaaga aatctgtctc aagagaaaat catcgactgc tttcgagaac tagcagaaga
    13201 gatggatgtt cagcaaataa cctttcagca tttagccaaa cgcttggata tcaaggctcc
    13261 ctcgctgtat aaccatttca aaaatattcg tgatgtcaaa acagcgctaa cagcgaaact
    13321 tctgcaggaa cttaatgacc agctgcgacg ggctcttgtg ggaaaaagtg gtcaggaagc
    13381 actgcagatc tatgcagaaa cttatcgatc ctttgctttt tcaaatcaag cagtgtatga
    13441 gttactgatc agtgtaccgc ataccaatga agctgtttta ttagatggaa tccatgaaac
    13501 gaatcagatc attttgcaga tatttgaggc ttttcaatta acgaaagaag aaaaagttca
    13561 tcgaagcagg gaacttcgta gtatgatcca cggttatctt tccttacgtt ttttaggata
    13621 ctttacaaaa gaaccaacaa tcgatccaga agacagctat ttttggatga tcaataactt
    13681 tatcgctact ttgccttaaa caatcagatt catttgtaac aattttaaga tgttcattaa
    13741 atgacccttt tataagagaa aattcttgca actttcttga aaagtgacta agatgagtct
    13801 agaaaagctg aaagaaggag cacgagtatg tatcaagtaa ttaccatgtt cggggacaat
    13861 gagccttggt ggttttttga agactgggaa gaagatattg aagaagaaga aacattcgag
    13921 tctttcgaag aagcacgaca agcgtacgaa aaacaatgga acgaaatcca tcaaaattat
    13981 gaatatatca atgcaaaaag taactttctc agcgcctttt ggaatgacgg agatgagcgc
    14041 tggtgtgaag agtgtgacga tgatttacag caatacaagg gacttgcgtt gttgaaaaat
    14101 catcagccca tcacagtaga aagcagaaag gaattttatg aaacaactaa ttctagcgga
    14161 aaaaccaagc gttgcgaaag acctaagcaa ggtgcttggt gctaatcaaa agcacaaaaa
    14221 ttattatgaa ggacccaagg tgatcgttac ttgggccctt ggtcatttat taggattgaa
    14281 aatgccggaa gatctgaata aggaatggca gagctggcaa atggacacgt tgccaatgat
    14341 ccctaaaaaa ctaggaatca aaccacttcc taaaacagga catcaattaa aagcaatcaa
    14401 gcagctggct cagcgaaaag atgtctcaga agcagtcatt gcgacagatg ccggaagaga
    14461 aggggaactt gttgctcgct ggatcttaga gtatgtgcat ttcaataaac cagtgaagcg
    14521 gttatggatc tcttctcaaa cagataaagc aatcaaaact ggattcaagc aactaagacc
    14581 tgcgaaagaa tatgatgcat tgtatgaatc tgctcttgcc cgggcaaaag cggactggct
    14641 ggtaggattg aatgtgacca gagccttaac tgtgaagtac gcggataatt tatcagcagg
    14701 ccgtgtgcag acaccgacac tagcattagt gagagaccaa gaaaagaaaa tcgagcagtt
    14761 ccgtccacaa acgtatttca cgatcacgtt gcaagctgga aaagaacaag ccaaaatgct
    14821 tcaaaaaaat caatttgctt taaaaacaca agaagaagca caaaaacttg ttcaattgat
    14881 cagtacaagc aaaggaactg tcacagaagt ccaagaaaaa ttaaaaacgg aatctgcgcc
    14941 attgccttat gatttaacag agatccaaag agaagccaat caacgctatg gctattctgc
    15001 aaagaaaaca ttaagtcttg tacaaagttt gtatgagacc cataagatcg ttacttatcc
    15061 gcgtacagac agcaaatatc tgacaaatga catgaaaagt acgatgaaag aacgattgca
    15121 agcagtcagc gactttgctc ctgaggtcaa gcagtatttg aaaaacggag ccatcgtcca
    15181 acagacaaaa gtattccaag acagtaaagt gaccgatcac catgcacttt tgccaactga
    15241 aaacagagca cgttatgaaa aattaagtaa tgaagagcaa aaaatctatc aaatgattgt
    15301 ctctcgtttt ttaggactgt ttgctcaacc ccataaagtc agccaaacaa aagttacggt
    15361 tgaatttggt aaagaacgat tcatttttcg gcaaaatcgt gtcgtccaag caggatggaa
    15421 aggcgaaact gaaactgaga cggaaacagt caaatgggaa aaagaaatga gaatcaatcc
    15481 cgatttcacc atcaaaaaag aactatctgc accgcctaaa ccattgtcag aagcaagctt
    15541 gcttggtcaa atggaaaaat acagtctggg aaccccagct acgcgtgcag agattatcga
    15601 gaaactaatc aaatctgaac tgatggaacg tacccctcaa ggactgcaag tatctcctaa
    15661 aggtaagcaa ttattagtat tggtaaatcc ctctttagta acaccagatt tgacagaaag
    15721 ctgggaaaaa gaccttgaag ctattgctgc aggaaagaaa caagcaggaa ccttcttgaa
    15781 tggaatcgaa aaagaaacaa aacgcttggt gaatgagatc aaatcaagca aacaagaata
    15841 tcaagatttc tcgattaccc aaaagaaatg cccagaatgt ggggccaatc tgcgcgagaa
    15901 aaacacacgt gacggcaaaa tctatgtctg caccaattca gattgcaact accgcagaag
    15961 aaaagatcca aaagtctcta atcatcgatg cccgcaatgt caccggaaaa tggaaatcat
    16021 cgaaggtaaa aacggtgctt atttccgttg taaatacgat ggaacgacag aaaaaatgct
    16081 tgataaaaaa gagcagaaga agaaaatgac caaacatgaa gaacgacgct tgctgaaaaa
    16141 atatgcacaa gcagaagaac cagaagaaag cccattagca gcagcactaa aagcagcaat
    16201 gaagaagtaa taagatatga aagaggttgt gatagaagtg gtcagcttca agaaataaga
    16261 aggaatttac gaaaattgct tctcaaattt ttgtgaaatt tcggcttatt tccgaagaag
    16321 ctacttctgg aacaccattt attcggaaaa aaggactgtg acaagacttt tgtcacagtc
    16381 ctttttcatt taaaaaaatg tttacttcat ttgacgtata ttattggtga ggcttcaatt
    16441 catttggctt cactttttct gtttctctta tgatataaac aggacgctgt ttcgtttcta
    16501 agaagatttt cccgatatat ttcccgatga ttccaagaca taacaattgg attccaccaa
    16561 tgaacaggaa gatcgagacc atggaaggcc atcctgatgt aggatcacca aacaacaatg
    16621 cccgcaaaat aatgaacaaa attgcaatac cagaaccgac acaagacaaa gcgccgataa
    16681 atgaagcaat cgttaacggt gcatccgaga agttcacgat accatcaatc gagtagttga
    16741 ataatttcca gaaggaccaa gatgtttcgc cagccacacg ttcgcggttt tcataagaaa
    16801 tatattctgt tttaaatccg acccagctaa ataatccttt tgaaaatcgg ttgtattctg
    16861 ttaattctag gacggcatcc accatttgac gagtcatcaa gcggaaatca cgagcgccat
    16921 cgaccatttc tgtatcgctg attttattga tcaactggta gaattttttc gcaaagaaac
    16981 tgcgtatcgc aggctcgcct tcacgagtcg aacgccgggt gccgacacta tcgacatcag
    17041 aagtagtgat ccggttgatc atctcaggaa gcagttcggg cggatcttgc agatcaacat
    17101 ccatcacggt cactaaatct cctgtagaag cttgcaatcc agcgtacaga gccgcttctt
    17161 tcccaaaatt tctggaaaat gagacaaagc ggacataagg ataagctgag gctagcttct
    17221 tgattgctgc taacgtaccg tcttttgatc cgtcgtcaat aaacaaatat tcgaactcat
    17281 gggcaccttt gaatttttca acctcatcaa aaaaaagagg aacagttttt tcttcattaa
    17341 aacatgggac aacaattgat actttcatcg tttctatcct ttcaatcgta tgtttatata
    17401 tggtttacat tcaaaattta ataaacctta aataacacct tttaaatttg attttatgga
    17461 aaagaacagc tattctttgg taaactaacg tatttagttt accatgtttc ctcggtttat
    17521 caaactttta agaacaattt ttattagttg gcaagtagtt ttggatagag aaaaaggagg
    17581 aagttccagt ctactgacca ggagcaaaca aaatgaaaat acacacacct atcggctttt
    17641 tatggttaga tgcagatgaa tccggattga ctgctgtatc ttttcagcca atttcagaaa
    17701 gcgaatcagc gaatcagcaa attttaacgc gcacacaaga acagtttgaa gaatattttg
    17761 cgggaaaaag aaaagaattt gatgttcctt tatctataaa gagaggtacg ccttttcagc
    17821 aaaatgtctg gaaagctttg tcgaccattc cttatggaga aatccgaacg tataaagaag
    17881 tagctgttgc tgttgatagt ccgaaagctg tgcgagcgat tgggcaggca aatcgaacca
    17941 atccattgcc aatcgttatt ccttgtcatc gagtgatcgg gcaaaatgga aaactgacag
    18001 gatatatggg aaacgcaata gatggggttg aaatcaaacg ccaattgttg ttgttagaag
    18061 gatatctgca aaattaaaaa cagttgtgat tcggacattg ttccatgcga gaatcacaac
    18121 tgttttttca tgttcattct aaactttgat cagccaaata aatagtaaag ccatcactgt
    18181 ttggagaagc ccgacagcta atccataaga taagaagcgt gtaccttctt tcatgaattt
    18241 tgcaaagtct aaacgcagac caattgccgc taaagcagtg atctcaaacc agctgctgat
    18301 aaaatgtgcc gattggtcaa taactacagg taaagaaaac aagctgttga ccaaacaaaa
    18361 gatcacaaac cctaatacat accaaggaag tggtaatttt cgttttttct gcgtatcagt
    18421 tggcgtttca aacagagctg gttgtttctt gtgcatgaat ttttcaaaca gatagacaac
    18481 aacaacgagc atcataatac gtagaatctt aaatagcatg gcgaattcga caacagaatt
    18541 gtcaagtaag cttgccccag cgactacttg accaacagat tggagtgtgc cacccaaaag
    18601 tgcacttttt tcaatgagat ttccaccaaa caactgtaat ccaaggaaag ggagagtcaa
    18661 catcatcaca gtacccagta gattgactag tgtgatgatc tgtccttttt cttcctcttt
    18721 cgcatggatg gaaggggcga tcgaagcaat agcagaagaa ccacaaacag cgtttcctcc
    18781 agccatcatc aaagaagaag tatcgctgaa ttgcagtttt ttaccaatca agtaagcacc
    18841 gatgatagtg actgacattt gcagtaacac aaaaacaatt ccttttatac caatttgagc
    18901 aatcgtttga gctgtgacag ttgcacctag taaaaaaaca gagaattcta atagtttgct
    18961 ttcagcaaat tttgtgccga ttgctagaac aggctgttta aaaaaagtat ttcctaataa
    19021 aatacccaat aaaatagcaa tagtcgctcc acctaactgt ggcagataaa tagcaagaag
    19081 cctgctgaag gcagcgatgg caaaagaaag cagtaaaccg ggtaagattt cgcggaccgc
    19141 tgaatccttt acagatttta ttggatgtga caatgatttc atttgatcgg ttcctttcgt
    19201 caataaatac taaaagcata ctcgaacata gtagcactaa accagtattt tgacaaaaga
    19261 acaagaggtc gtgacaaaag tcttgtcacg acctcttgtt tcgaataaac ggtgttcaaa
    19321 agccagtttc tcggtattaa gccaaattta tgcaaaaatg tggaaagcca ttttcacaaa
    19381 atttttctta atactcgaaa ctaagcggct ttttcacgac ctcttggtaa acggtgttcc
    19441 agaagtagct ttttcggaaa taagccgaaa tttcacaaaa atttgagaag caattttcgt
    19501 aaattccttc ttatttcttg aagctggcca cttcttttac gacctcttgg taaacggtgt
    19561 tcaaaagctt gcatctgcgg taataagccc aaccaattca aaaacatgaa cagctgtttt
    19621 tgtttggccg ttcttattac ttgatgcaga acagcttttt cacaacctat tgaatcgatt
    19681 attaagcgat ttttttagaa acaaattttc catgtttgct atgttcttta attaagaaac
    19741 gtacgtaatg agcaagaacg tagaagacaa ctacacttgc aaacagacat tccgcaactt
    19801 ttaagaaatc caatacgcta ctatagatac ctaaactgtt gaagtaatga acagtagtaa
    19861 atatagctgt tgcagagata acaagcggga atgtaaaggc tgcatagctt ggataaaaac
    19921 gaagcttgaa caaacgtgga agcatagaca gcacaacgaa atacaatact tgagataaag
    19981 cgaacaatgc tacgacaaga taaaaattag gttcagcaaa gtttttaaga taacctgtta
    20041 ggcaaagaga accaggtgct gcaacgatcg taatcagcgg acgtgtcgga tgagccatat
    20101 ttcttacgaa aaagacacgt ttgatgatga tcggcaataa gaacgcatag cagatcaatg
    20161 aaatccaaaa gaaaattttt ccaataagcg ggaagaagtt cccggcagtt acagtgatga
    20221 ccccaaatcc aacataaatg ataaaccagc ttggataaat tgcttcaatt gaaagatcag
    20281 ctttgataac atggtgataa ttgaaataaa agacaaggat cacttggaag ataacagcgc
    20341 acagccaaat gaatttgaca attggtgcaa tactttcata tgaaacaaaa taagtacaaa
    20401 taaccatgag tgacatcgta aaagttggtg aaacggaggc gatgattgga tcatgcaacg
    20461 tttgtttcgt gtgttcgaat aagataaaca atttaacaaa aatgaatatc ataagaatca
    20521 tagagatacc gccagttaga ttgcctaaag caacatggtg atagtcttta aacagatttc
    20581 caagggaagc taacccaagg atcaacccgc agatcgggat cggaataatt ttcaaaaagt
    20641 tcttcatata taatattctc ctttttattt aatagatttt agtaccaaga tgaacgaata
    20701 cattgtaaaa tgaataggaa tgtatgatta cgagcaagca gacagctgat tgtcttcatc
    20761 taagttacta gttggatcag caactgtttt gttgtacatt tctattatag atggtttttt
    20821 gtatatttaa atcaaatagt ttttatagta attatcataa aaagttatgg aagtggtgcc
    20881 atgtttaagt tattaacgac ttttcgagca gtatatgaaa caagaaattt ttccagagca
    20941 gcagaagtac tgtttttatc tcaaccagca gtctcgaatc aaatcaaaca gttggagcgg
    21001 gaattgaata tcgatctttt tcaacgtaac ggaagaaaag agatgcttcc aactaagcaa
    21061 gccgacttat tatatgaacg gacactggtt ttgttagaag aatgggaaga aactcagcaa
    21121 aaattgctca atgaacaaaa tgaagaagaa gtatgtcgaa tcgttgcttc ccacaccttt
    21181 gctgtctatc ctttgcctca gttgatgaaa catctgatca aaaggtttcc taaagtgcat
    21241 tttgagatcg aaatcgcgaa ttctcatgaa tcattagaac aagtagcgaa acatgaatcc
    21301 gattttggtt ttatcgaaaa gccattagag acttccggcg tacaaagata ctctttgatg
    21361 gaagaccagc tagtcatcgc tggggaccca aaaagcaaat tgtggcttgt aagagaagca
    21421 aactctggtg tttttcatta cacgcagcgt tattttgaag agatgaatat ccaagataag
    21481 aagatgatcg tgaaaagtaa cgatgtaatt gttgcg
//
LOCUS       DACEXQ010000022        20501 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.022, whole genome shotgun sequence.
ACCESSION   DACEXQ010000022 DACEXQ010000000
VERSION     DACEXQ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.022"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..272
                     /locus_tag="IXH04_000669"
     CDS             <1..272
                     /locus_tag="IXH04_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851301.1"
                     /translation="SSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEAEEFVYPFRG
                     YVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            349..762
                     /locus_tag="IXH04_000670"
     CDS             349..762
                     /locus_tag="IXH04_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAQ1851302.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            775..1161
                     /locus_tag="IXH04_000671"
     CDS             775..1161
                     /locus_tag="IXH04_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851303.1"
                     /translation="MWKDYVSLKELKKDLVFKRIVEWSESELILEDGTKMEVVCSESD
                     CCAWAEGEFKNVKLDAVITDIKIFDKGNRLYNGDGHTSYAEVVVYHNRNEIAKAECTA
                     NDGNGGYYYSVCALKVKDKLCIVTDA"
     gene            1712..1936
                     /locus_tag="IXH04_000672"
     CDS             1712..1936
                     /locus_tag="IXH04_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851304.1"
                     /translation="MRTYWYVSLNNKYPKPMKGQHRRVVMSVQMKAKYSIVEMIREAT
                     PVEIDYCKLVYCGCGRWKEDHVQKNISKYI"
     gene            1954..2382
                     /locus_tag="IXH04_000673"
     CDS             1954..2382
                     /locus_tag="IXH04_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851305.1"
                     /translation="MEKSDTKANRYKPTAAEKKLLEVLINPDNLGKPVQELCSLAKIG
                     RTKYYDAMSKDGFVDLVNETTMDLIKGKASDVLNATYKYALTEKGHQDRKMILTIAGI
                     YADKQELKHSGGVDIRKQYEKMSDEELEELVKRYEKINDS"
     gene            2360..3778
                     /gene="terL"
                     /locus_tag="IXH04_000674"
     CDS             2360..3778
                     /gene="terL"
                     /locus_tag="IXH04_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HAQ1851306.1"
                     /translation="MKKSTTLNRVEYAVVQRELAIRKARKSFWLFCQLLYPEFYKDDR
                     LYLKDLCHTLQDFYEDKIDKKILVINMPPRHGKTFTARLFVLWMFGQNPKTKIITGSY
                     NQILSSLFAQQTRDGIMVENENVKAEYFKDIFPEVQIKYGDAAKGFWSLDGSEEKNYL
                     ATSPGGTSTGIGANYVIVDDIIKTAEEAYNERVLDSHWEWYNNTLAQRMERPRKQILI
                     MTRWSSNDLAGKMLTRRKNNVHQICYKAVQEDGSMLCNEIMTHDEYLDVVQEMNVDIA
                     EANYQQEPIDQKGRLYQKFLTYDTLPDNIIKIWNYTDTADKGADYFASPVFAETSDHD
                     AYIIDVMYTKEPMETTEIAHANMIIRNRVNKVRIEGNNGGRGFRRNSERITKERGYRG
                     AVFEDFHQSDNKLSRILANSAWVENHVYYPDDWATRWPDFYEAMVSYQREGKNAHDDA
                     PDAVTGIAETIIEADDFEFIAY"
     gene            3790..5325
                     /locus_tag="IXH04_000675"
     CDS             3790..5325
                     /locus_tag="IXH04_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010779952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ1851307.1"
                     /translation="MFYSGITLKKYKRLRTKYSTQIEEGLFDPNAFMEDMKPFFDDRE
                     RKYLAYTSEKNEIDSREKPKTKIIKVNNKIHAGMYSVVVDQAVNHFTGIPIKWDYDVS
                     EQKRTFIQRMRDKLLGNDISAPSTPEEFETLTRQLSTMRFGMLDPDTATFQGATGLAF
                     RLLEPVETNDGWELRASNIEPWKAEKYENGAVYIKEKYDPYQKKFYEEMKVVTPKSIF
                     TYARYTDYPGTVQGAFKTLGNVPNPLGVINLTEFKNNTNRYCDFEVAEEISDAIDRVL
                     SDQQNEIEQFKLAYMLITGADLSEDKAKKMMEQLGIINLKDPNAKAGYVTKDLQKDFN
                     EYHFDLLKKQFYTVCKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAEVMAAYLDFAEKVTIDPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMIDVESRQSYPDGEYGGGIVESNRNEVLDQTSRTGRFSKTQNGKSNAK
                     ETK"
     gene            5405..6283
                     /locus_tag="IXH04_000676"
     CDS             5405..6283
                     /locus_tag="IXH04_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HAQ1851308.1"
                     /translation="MMEFYSTSDQKKYREYVEKNYKSLKMYDAKYKEFIEEYFPSFDY
                     AKVNRLLQMRSDIFRILAESAIDADVNKYFSDRLEDILQRSYVSNANVFAQILNVDLP
                     NYLSKEELERYLNYPWSGKTFSRRLWGNISSLEQKLSNAITTSVASGEGVLVALRTMR
                     EDPEICDMFKREESKYNKAIENLVRTEYAKFAQDGIEKSYIETGVDEYNVLTAKDEKV
                     CSICGGKAKNNPYKLSEAIIGENRAPFHGRCRCTDVPNMPKLGKDIDEEYERLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            6285..6602
                     /locus_tag="IXH04_000677"
     CDS             6285..6602
                     /locus_tag="IXH04_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851309.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYENMGCIVVRTDYKEGK
                     SSN"
     gene            6651..7352
                     /locus_tag="IXH04_000678"
     CDS             6651..7352
                     /locus_tag="IXH04_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HAQ1851310.1"
                     /translation="MVEWWGATIKTQSNAGRENESWGERRMIMKHKSLMPMNLQYFAE
                     GDDQKFSFDDFKSFVESNEEAQKFIQSQSQSVADKQLEAWKQNNLDKIKQDTIKEYEE
                     SKKNKSPEQIQLEKLQAEFEAEKALRVTSDNKAFVAEQIAGLELDGELKESISQFMLN
                     NLVSSDTDFTKNAVEGFTSVLNAINEKHADALKELQMKSAFGGTQQSNNQVQQNNETL
                     TNPEEQLGQILQQFN"
     gene            7367..8257
                     /locus_tag="IXH04_000679"
     CDS             7367..8257
                     /locus_tag="IXH04_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HAQ1851311.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            8280..8495
                     /locus_tag="IXH04_000680"
     CDS             8280..8495
                     /locus_tag="IXH04_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851312.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            8507..8848
                     /locus_tag="IXH04_000681"
     CDS             8507..8848
                     /locus_tag="IXH04_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851313.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            8848..9219
                     /locus_tag="IXH04_000682"
     CDS             8848..9219
                     /locus_tag="IXH04_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851314.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            9212..9607
                     /locus_tag="IXH04_000683"
     CDS             9212..9607
                     /locus_tag="IXH04_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAQ1851315.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGAVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            9609..9986
                     /locus_tag="IXH04_000684"
     CDS             9609..9986
                     /locus_tag="IXH04_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851316.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNKPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            9987..10595
                     /locus_tag="IXH04_000685"
     CDS             9987..10595
                     /locus_tag="IXH04_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ1851317.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            10595..10936
                     /locus_tag="IXH04_000686"
     CDS             10595..10936
                     /locus_tag="IXH04_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851318.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            11178..13967
                     /locus_tag="IXH04_000687"
     CDS             11178..13967
                     /locus_tag="IXH04_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HAQ1851319.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANSFVLIIGAVVGAI
                     AVFGYFMATNEEFRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNEIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNIFVSYFTNIYFAALNI
                     WTGFKLTLINIWNEVVNQAKSIWLNIKYFFINLWIDIKYFAIQKWIELKFSIIQTWID
                     LKYNAITTWNNIKQFFKDTWKNIKDTAYNTWISIKNTMINTWNNIKDSFWNIVTGIVN
                     SAENAWTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTI
                     KGIAGNIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTP
                     SVQPVVNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQ
                     MKRDAGAVGGTLYAT"
     gene            13957..14697
                     /locus_tag="IXH04_000688"
     CDS             13957..14697
                     /locus_tag="IXH04_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ1851320.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYRNVTRKIECYYKAPSIDVVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            14694..17456
                     /locus_tag="IXH04_000689"
     CDS             14694..17456
                     /locus_tag="IXH04_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAQ1851321.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCEIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLAEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRLIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     SLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFTSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSAGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWRIYDILCNNGFTK
                     QSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWDGKVYVQNLF
                     NAAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGFKACTDPRQAAYAFERNYE
                     RPAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYS
                     GYLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIIN
                     WARGGQVGSWFADGTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAISS
                     IVIPPK"
     gene            17469..18377
                     /locus_tag="IXH04_000690"
     CDS             17469..18377
                     /locus_tag="IXH04_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HAQ1851322.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETRIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            18377..18994
                     /locus_tag="IXH04_000691"
     CDS             18377..18994
                     /locus_tag="IXH04_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851323.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAESYTNERIPNFTFEKIGEV"
     gene            18998..19447
                     /locus_tag="IXH04_000692"
     CDS             18998..19447
                     /locus_tag="IXH04_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851324.1"
                     /translation="MTDIVKIKQSGVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLTNITFEKVGTV"
     gene            19447..19971
                     /locus_tag="IXH04_000693"
     CDS             19447..19971
                     /locus_tag="IXH04_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851325.1"
                     /translation="MADIVQLKEDGVAKYLKTHAKGIDGVEGVLVKATGNETVLGTKN
                     FKDGLQFNGLPVQAGMIERAITLADRSDTTNVTDVNGKIIRIGNIVFLTFNFKCGTWP
                     EGSETRWILKIPDGFKRDQGYPAQTALSLVRNASQPADARAFIDQSSIIQAKSGSGSS
                     YISGMWITQDPWPA"
     gene            19999..20406
                     /locus_tag="IXH04_000694"
     CDS             19999..20406
                     /locus_tag="IXH04_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851326.1"
                     /translation="MKTIYKVLYPMGYEEHQVEDNFPTSLPFVEVPPILFEKKEDETD
                     EGFGRRQQSQFFNFTENKWEEAVTQDYSKKLELLENLSIGLQVDNAALKKSNEELTEK
                     ADSMAQLNAKLMLNDLTINKKIEAIEKQIGGAS"
     gene            20406..>20501
                     /locus_tag="IXH04_000695"
     CDS             20406..>20501
                     /locus_tag="IXH04_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAQ1851327.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRQFVPLCITDE"
BASE COUNT         7304 a         2906 c         4368 g         5923 t
ORIGIN      
        1 ctagcagtca cggtatcggc ggtttaatgc tagatatgcc gacaaagaac cctaaaacac
       61 gcaagaaatc aaaattcaaa gtcggtgaca tggtccgctg tgaagcagaa gagttcgttt
      121 atccgttcag aggatacgtt aaaaagatac tgtcaaactc agcaatcatt cgtattgaaa
      181 atacaatgaa gtgtgatcgt tcgactgcaa aacacaagca ttatttagct gtagcgagat
      241 tagttgacat ggaagtaatc aagagcaaat aaaaaaagcc ggatcgctcc gactgattca
      301 ataaatccaa cacatttatt atatcacata aaggagcggt ttgacttgat gcaattgtta
      361 cgagaggtag atttcaaaca gacaagatgt aatgcgagag atgtgctgaa gaactttcgg
      421 cgtttggagc ggatggcagg tcgctctttg atagatatta agtcgccgat tataacggat
      481 atgccgaagg caccgaagca tggcaataag gcagaagacg cgattattca gatgatggat
      541 atagaaacgg agagagatgc gattctagcg gctttgatgg ctcttagtct gattagtcgt
      601 cagatactct actacagctt ctgtgtgcca gatagcttct caaactacag gattagccgt
      661 gaagtgggtt attcagaaag aagcatacaa aggatgaagt cggaagctct aatagagttt
      721 gcagaagcgt ataaacacgg aagaataatt gcttataaat agggggacca tataatgtgg
      781 aaagattatg tatcgttgaa agagttgaaa aaagatcttg ttttcaaaag aattgtagaa
      841 tggtcagaga gtgaattaat tttagaagat ggaaccaaaa tggaagttgt atgtagcgaa
      901 tcagattgct gcgcatgggc cgaaggtgaa ttcaaaaatg taaagcttga tgcagtgatt
      961 acagatataa agatttttga taaaggtaac cgtctctata atggtgacgg acatacctct
     1021 tatgctgaag tcgttgttta tcataatcgt aatgagatcg ctaaagcaga atgcacagcg
     1081 aatgatggga atggcggcta ctattatagt gtttgtgctt taaaggtcaa agacaaactt
     1141 tgtatagtga ctgacgcata aaaaaatggc ggttttttgg cggaatgatg gcggttttta
     1201 gccatttatc agtgatatta tggtagtgtc gaaagattag tgataggtct gagacaaaat
     1261 aataataaaa ggaacatcgt tttattattg tttcacaatt aagcttcgat agacagcaac
     1321 ggaaatatta agaataagga tgtgaatttt aactccttct aaattgttct tattatctat
     1381 catccgttgc tgtctattgt tacatacaga tcgcttaggc ggtcttttta ttttggccga
     1441 aaacctacat tttcgatggc cgattattgg aggaggaata gcatgttcaa actatctgaa
     1501 atcattaaga agtccgacgt tgagaaatta gagatgttaa aaaagaagtt gaagaaatag
     1561 cagtctcttc gtgaggctgt tttattttgc tcacaaaaat agaccactat cgggtaatag
     1621 tggtcaggaa ttaaatgaaa aagatgttag ggttgttagc taagtataac atcataacgc
     1681 ttacaaaaca atgcaagaag gataaattat tatgagaact tactggtatg tgtcattaaa
     1741 caataaatat ccgaagccaa tgaaaggaca gcataggcgt gtagtcatgt ctgttcaaat
     1801 gaaggcgaag tattcgattg tagaaatgat cagagaggca acgccagtag agattgatta
     1861 ttgcaagcta gtctattgtg ggtgcggccg ttggaaagag gatcatgtac aaaaaaatat
     1921 cagcaagtac atatagagag gcggtgatta aggatggaaa aaagcgatac gaaagcgaac
     1981 agatacaaac ctactgctgc agagaaaaaa ctcttggaag tattgataaa tccagataac
     2041 ttaggcaaac cggtgcaaga gttgtgttca ctggcgaaaa taggtaggac caaatattac
     2101 gacgccatga gcaaagatgg ctttgttgat ctagttaacg aaacaacaat ggatctgatc
     2161 aaaggaaaag cttctgatgt ccttaatgcc acttacaagt atgcattgac tgaaaaaggt
     2221 catcaggata ggaaaatgat tctgactatt gctggaatct atgcagacaa acaagaactc
     2281 aaacattctg gcggcgtcga tatccgaaaa cagtatgaga aaatgtccga tgaagaatta
     2341 gaagagttgg tgaaacggta tgaaaaaatc aacgactctt aatcgtgttg agtatgccgt
     2401 tgttcaaaga gagctagcca ttcgaaaagc taggaaaagt ttttggctat tctgccagct
     2461 tctttatcca gagttttaca aagatgatcg gttgtatttg aaagatttat gtcatacgct
     2521 acaagatttt tacgaagata aaatcgacaa aaaaattttg gttattaaca tgccgcctcg
     2581 acatgggaag acttttacag cgcggctatt cgttttatgg atgtttggac aaaatccaaa
     2641 gacgaaaatt attaccggat cttataacca aatcctgtca tctttatttg cgcaacaaac
     2701 acgtgatgga atcatggttg aaaacgaaaa tgttaaggct gaatacttta aagatatttt
     2761 tccagaggtg caaatcaaat atggagatgc tgcaaagggt ttctggtctt tagatggttc
     2821 agaagaaaag aattatttag cgacttctcc tggtggaaca tccactggta ttggcgcgaa
     2881 ttatgtaatt gttgatgaca tcattaaaac cgcagaagaa gcctataacg agcgtgtttt
     2941 agattctcac tgggaatggt ataacaacac gttggcccaa cgaatggaac ggccacgtaa
     3001 acagatattg attatgacac gttggtcaag taatgattta gccggaaaaa tgttgacgag
     3061 acgaaaaaat aatgttcatc agatctgtta taaagcagta caagaagacg gctcgatgtt
     3121 gtgcaacgag attatgaccc acgatgagta cttagatgtg gtacaagaaa tgaatgtaga
     3181 tattgcagaa gccaactatc agcaagaacc aattgaccaa aaaggcaggc tatatcagaa
     3241 gtttcttact tatgatacgt tgcctgataa cattatcaaa atatggaact acaccgacac
     3301 cgccgacaag ggtgccgatt actttgcgtc tcctgttttt gctgagacgt ctgatcatga
     3361 tgcttatatt atcgatgtca tgtatacaaa agagccgatg gaaacaactg aaatagccca
     3421 cgctaatatg attattcgca atcgagttaa taaggtacgg attgaaggaa acaatggcgg
     3481 gcgtggattc agaagaaatt cagaaagaat tacaaaagag cgaggctatc gaggtgcagt
     3541 atttgaagac tttcatcaat cggataataa actgtctaga atactggcga atagtgcttg
     3601 ggtggaaaat catgtttact atccagatga ttgggcgaca cgttggccgg acttttatga
     3661 ggctatggtt agttatcaac gtgaaggtaa gaacgctcat gatgatgctc cggatgcagt
     3721 tacaggaatt gcagaaacga ttatagaagc agatgacttc gagtttattg cttattagga
     3781 ggtgggtctt tgttttattc tggaatcaca ttgaaaaaat acaaacgttt aagaacgaaa
     3841 tattcaacgc aaatcgaaga aggattattc gatcctaacg cttttatgga agatatgaaa
     3901 ccctttttcg atgatcgaga aagaaagtat cttgcttata ctagcgagaa aaacgagatc
     3961 gacagtcgtg agaaaccaaa aacaaaaatc attaaggtca ataacaagat tcatgcggga
     4021 atgtattcag tggtcgttga tcaagcagtt aaccatttca caggaatacc aattaaatgg
     4081 gattatgatg tttcggaaca gaaaaggaca ttcattcaac gaatgagaga caaattactg
     4141 ggtaatgata tcagcgcccc ttctacccca gaagaatttg aaacactcac gagacaatta
     4201 agtactatgc ggtttggaat gttagacccc gatactgcaa catttcaagg agcgacaggg
     4261 cttgctttca gattactgga gcctgttgag acaaatgatg gttgggaact aagagcaagc
     4321 aacattgagc cgtggaaagc tgaaaagtac gaaaacggcg cagtgtacat taaagagaaa
     4381 tacgatccat atcaaaagaa gttttatgaa gagatgaagg ttgtcacgcc gaaatcaata
     4441 tttacttatg ctcgatatac tgattaccct ggcacggtac aaggagcatt taaaacgctg
     4501 ggtaatgttc ctaatccttt aggcgttatc aatcttacgg agtttaaaaa caatacaaac
     4561 cgttactgtg actttgaagt tgcagaggag attagtgatg ctattgatcg tgttttatct
     4621 gatcagcaga acgagattga acaattcaag ttagcttata tgctgatcac tggggcagat
     4681 ttgtccgagg ataaagccaa gaagatgatg gagcaactag ggatcatcaa tctgaaagac
     4741 cctaatgcga aagccggcta cgtgacaaag gatctacaaa aggatttcaa cgagtatcat
     4801 tttgatcttc ttaaaaagca attctacacg gtgtgtaaag caattgattt caatgacgaa
     4861 gtctttaagt cgaatagttc tggggaagcc cgaaagtggc aaatcattag tttagaagct
     4921 aagacaaata ccaaagaaca gtactttcga gaaggattga aagagtgtgc cgaggttatg
     4981 gctgcttatt tggattttgc cgagaaagtt acaattgatc cggaaaagat cattttcacc
     5041 ttctctcgtt cgcttccaac agatttaagc tacctagctg aggcattgcc taagttggca
     5101 ccatacgttt ctaagaggac gattcaaagt cagatcccgt tcgtcacaga tgttgattat
     5161 gaaaacgaaa tgattgacgt cgaaagcaga caatcttacc cagacggcga gtatggaggt
     5221 ggcatcgttg agtcaaatag aaacgaagta ctggaccaaa cgtcgagaac tggaagattt
     5281 agcaagactc aaaatggaaa atcaaacgct aaagaaacta agtagtgtct ttcctgatgc
     5341 gttgaaagaa attcaagcgg agttattatc ccaagctgat ttacacgata ttacctatgc
     5401 tgagatgatg gaattttata gtacaagtga tcagaaaaaa tatcgtgaat acgttgaaaa
     5461 gaactacaaa tcactgaaga tgtatgatgc aaaatacaaa gagttcatcg aagaatactt
     5521 tccttctttc gactacgcca aagtgaatcg gctgcttcaa atgaggtcag atatttttcg
     5581 aattcttgca gagagtgcaa tagatgcaga tgtgaataaa tatttttcgg atcggttaga
     5641 agatatcttg caacgttcgt atgtatcaaa cgcaaacgta tttgcccaga ttttgaacgt
     5701 agatttgccg aattacttat caaaggaaga acttgaacgt tacctgaact atccttggtc
     5761 aggtaagaca ttctcgcgtc gtttatgggg caatatttca tctctagaac aaaagctatc
     5821 taacgccatt acaactagtg tagcaagtgg cgaaggagtt ctcgtggcat tacgaacaat
     5881 gcgagaagac ccagaaatat gcgatatgtt caaaagggag gaatcgaagt acaacaaggc
     5941 gatcgaaaat cttgtgagaa cagaatacgc taaattcgct caagatggta ttgagaaatc
     6001 ttatattgaa actggcgtag acgaatacaa tgtcttaact gcaaaagatg aaaaggtatg
     6061 ttcaatatgc ggtggcaaag caaagaacaa cccatacaag ttgagtgaag cgatcattgg
     6121 tgagaaccgt gcgccttttc atggccgatg cagatgtaca gatgttccta atatgccgaa
     6181 attaggaaaa gatattgacg aagagtatga gcgtttattt ggcgatttat tagatgagtt
     6241 tgcacatgat tcttttggaa ttaatttgaa acggaggaag tagaatgaaa gatttttttg
     6301 aagcagtact aacaattaat gtaaatgctg atattgcaga agcctacaaa acagctattg
     6361 agtctgagaa ccatcctaat ggcttgagag accattggaa tggcaattat gcctacgtgg
     6421 ttattggcga tcaaactgtt aattatcaag ataatactcc agttgataag aataccgtta
     6481 atttaacgat tcaattatta tctcattcat taccaaattt aaaagaaaca gttgattggt
     6541 atgaaaatat gggatgtatt gttgttagaa ctgactacaa agaaggaaag tctagtaatt
     6601 aggctttttt attttgtccg aaatgacgtt aaactagcgc aatactgggc ttggttgaat
     6661 ggtggggcgc aactattaaa actcaaagca atgcggggcg tgaaaacgaa tcgtggggcg
     6721 aaaggagaat gattatgaaa cacaaatcat taatgccaat gaatttgcaa tactttgccg
     6781 aaggtgacga tcaaaagttt tcttttgatg acttcaaatc ttttgtggaa tccaatgaag
     6841 aagctcaaaa atttattcag tcacaatctc aaagtgttgc cgacaaacaa ttggaagctt
     6901 ggaaacaaaa taacttagat aaaattaaac aggataccat caaggaatat gaggaatcta
     6961 agaaaaacaa gtcacctgag caaattcaac tggaaaaact acaagctgaa tttgaagcag
     7021 aaaaagcgtt gcgtgtgaca agtgataata aagcatttgt tgcagaacaa attgctggat
     7081 tagaactaga tggagagtta aaagagtcta tttctcaatt tatgctaaat aatcttgtta
     7141 gttcggatac agatttcact aagaatgctg ttgaaggttt tacaagtgtt ttgaatgcaa
     7201 ttaatgagaa acatgcagat gcattaaaag aactacaaat gaagtctgca tttggtggaa
     7261 ctcaacaatc gaataaccag gttcagcaga acaatgaaac attaacaaat ccagaagaac
     7321 aattaggaca aattcttcaa caatttaact aggagagtga aaaattatga aaaaaacatc
     7381 tttaaataat ttagagtatt tggatatttc accagcgatt aatgctatgc aagtaccaaa
     7441 tacacctttt ttaagctatt tacttggtgc tggaaaaaca gagccagcaa actcgacaga
     7501 aattaaatgg cgtgaatatg atatcaacaa cgatgattct tctgaaaaac ttgagggcgg
     7561 agaatatcca gatgctgaat caggtcgaac ttggtttaac aactatactg aaatttttag
     7621 aaaatcaacc tctgtatctg gtacattaga tgctattaat gtgaatggag tcggaaatga
     7681 attaactaat caagtagcac tacgtggtat ggaaatgaaa attgatttga accgaaaatt
     7741 gattactggt gtaaaagctg atgaaaatgg ttctaaaggt cgtcgaatga atgggatttt
     7801 gaacttgatc aattcagcaa ataaggcaga aacagccact gcgggtgcag taacaagaaa
     7861 agatatcgat actttattta aattgatgta tgaaaaaggt tatatgggag aaaaactatg
     7921 cttgatttct ccagatatgc aggagttaat gactgatgag ttagatggaa aatcaacaaa
     7981 aattgttcag ttcggggaaa gagtaacttt tggattgcaa attggaaata tcgtgtctaa
     8041 ttacggtaca ggtattgctc tactagaacc atcattgcca aaaggaacaa ttgccgcaat
     8101 cgatactaat tatgtgaaac tacgtccatt acgtgaatgg agagcagaag aacttgcaaa
     8161 aacaacggat tctagacgta tcggtcttgt aggtgaatac tctcttgaat acaatgcttc
     8221 aaactctggg gcaattttaa atttaaagtc tgaataaaag ggagttagta ctcccttttt
     8281 tgataggagg aattacagtg gtaaaaaaag atgaaactaa aaaagatgaa gtcgtgaaat
     8341 atagagtagg taaaactaaa aattttgttg ggtttgttca tcctaaaact cgtagattta
     8401 tcacagcaga ttcaaataac gaatttatca tttctataga tgataaagaa gcaattgcaa
     8461 ttttggaaga tgcaattgat gttaatgaaa tttaggaagt gatctgatgg atgaatcgct
     8521 aaaaacggaa atcattgagt ctacaaaaga agattttcca gatttgagtg aagaacgcat
     8581 aactaattta ttagaaataa ttttgctaga aattgaatca tacaacactt gtaaaaatga
     8641 tatttcatgg gaaaagttaa aaagcgtgat tagtgaagtg ttgtataaaa taataaaaaa
     8701 tgaatcagaa aaaacagtat cttcagttag acgtggcgat acaacgatta gttatgcttc
     8761 aacaacaaat gatgttagtg agttacttct aggctatggt gatttgatac gaagagttat
     8821 tggttgtgga ggattggagt ttttttaatg aatgaagcag atattttaga aactacttat
     8881 gaagatagtt gtattattga aagactgacg gacattgaag attctaatac aaatattact
     8941 attcaagatt acaaaaaagt atatgataat cctatttctt gcgctctttc acaaggtcaa
     9001 atcgatggac tagcagtcat agaagatgga gagatggtaa atgtttcaac tgacacatat
     9061 aaattatttg ttcatcctaa gattaaacta aaaaaaggag atcgaataac aataactcaa
     9121 aaggccagtg gcttaatttt ttctctattt gctactaagc ctttttacta tcctagtcat
     9181 tgtgaagtga atttgatagg aagtgagaaa aatgggtaat ctcaaatatg aatcaaatgc
     9241 agaaaagatc attgagaatt ttaaaaatat gactgtaatt gctcaaaaag aaggaatatc
     9301 cttcgttaac gattcaatga ataaagtcgt tagtttagct aaaccattaa ctcctgtaaa
     9361 atcaggtaat ttaaggcgag gatatcgtgt agtaaaagct agaaaactat caagcggtcg
     9421 aattgttgga gcagttataa acaatgaaca ttattttaaa tatgtagaag aaggtcatag
     9481 gactaaaaat ggtggctttg tcaaagggaa attcatgtta actcgtgcaa cgaatcttgc
     9541 aaatatgtct tatattcctc gaagatttaa acaaatggca ataaaaatcg ttaagaaagg
     9601 aaagtaacat gaaagatgaa atcattgctg caatcagtag caaattaaaa gaaatctatc
     9661 ctgatgggac aatatatctt gattcagtta tgcagtcaac taaagatttt tatttcgttt
     9721 tatcagtaat ggaatctgga actgaaaatg taggaattga tattcaaaat gtttctttct
     9781 taattgatat tgcattgatt gataataaac ctaatagaaa tttaattaat gaattagtct
     9841 cacgttgtgg gacttttttt aatacgatta caatcgatga gcaaatatta tttccaaaag
     9901 cctatttacc tgatgaagca gatggtgttc aacatattcg ttttacatta gaatttccac
     9961 aatatattga atggagtgaa agataaatgg gagaaaaaag aagtaaaacc ggaattattt
    10021 ctgtcgaaaa acctacttgg tttcctttgg aagatgagac agggaatttc ccggtatatg
    10081 gtactgcgtt gccaatggga acagcagtaa gtatcaaacc aacagctaat tatgaaacaa
    10141 cacaagatta tggggattca gtggttcaag atcaatttac agcttttggt ggtgcagaag
    10201 ttgaactgga agcaaatgga tatactcata aagttttatc tacaattact ggagggaaaa
    10261 ttgttaaagg tggtgccttg cgttctggag aagatattgc gcaagatggt gcgtttgcat
    10321 atagacgtaa aaaatcgaat gggaaatacc gttatacggt cttttataaa ggacagtttg
    10381 ctctagattc agatgaaacg tcaacgattg aaggaagtaa agtaagtttt actcatccaa
    10441 cttggaaggg ttcatttgta gatgtaccag gacttggata tatgtattca gttgatgaag
    10501 atgatgaagg cgtggataaa gcaatgatcg gaaattggtt tacaaaagta gcaattccaa
    10561 ttgaagaagc agaactttca ggaggtacag aataatgtca aaatatcaaa caacgattaa
    10621 attaacgaaa aaagatgaag aaggtaaata tgagcaagta caatttaaat ctgctgaatt
    10681 tttaccagga acagttgtag aagatgctgc aggagttatg gaagaaatgc aaacagcgac
    10741 tgataaacaa tcagttaaaa aagctttaag tcgtgcttat tcatttattg cagatacttt
    10801 atttgaggga caatttactg gtgaagatta ttgcaaagga attgatgctc gtgagattgc
    10861 ctcattgaca ggaaaattat tgaagtctgt tactgcaggt tttgatgaaa cttatacaga
    10921 gacgaaaaaa aagtaagtga ggcgctcaag tcaccttcat ttaagtattc gattacttac
    10981 cgagaattag atataaagac gcaattactc gaagcaggtt ggacgttacc agaaattgaa
    11041 cgtactgact tggatgagtt aatgcgtctt tttgctttta gagatgcggt aaaagagcat
    11101 gaagatgttg agtactatga taatttcact caattttagg aggtgatact ttgaataacg
    11161 atgacctaat tctgaagatg atactagatg aatctggttt tactgccggt atgaataatg
    11221 ctgtcaaaaa gttgaactct tttgatgaaa caatcgaaag aaaaagtaga aatagtggca
    11281 gttcattagg tagtatttgg aaaatatttg ctggaagttt tctagctagc ggagtaacta
    11341 gaatcgtagg agctggcttt gatctaatca agggttccat aagtggagcg gttgatcgag
    11401 tagatacgat gaataacgct ctacgaaatt tccaaaatat gggatttagt aattcagaaa
    11461 ttatgaagaa tatcggaaag aatgggcttt tatctcaagg tattcaagga cttcctactg
    11521 ctttgaatga tgcgataagt catgttcaac ttcttgcttc ttctacaggt gatttaactc
    11581 gttcaactca aatatttaaa gctttaaatg atggaattct tggtttcggt ggttcaactg
    11641 atcaagttaa tgaagctgtt attcaattat ctcaaagttt ctcaaacgga aaagtagacg
    11701 cacaaacttg gaactcaatg atcaacgctc aacttggacc tactctttct gctattgcta
    11761 aaaagatggg aattacaatg ggagatctga aagaaggttt gtctcaaggt aagatttctg
    11821 ttgaagaatt ccaaaatcaa ttaatagaaa tggataccaa aggtggcgga ggacttaaat
    11881 cattaagtca aatcgctaaa gattctacta agggaattaa aacctctata caaaatgcaa
    11941 aaacagctgt tacacgtggt gttggtgagg taatagaagg attaaataaa gcattagtag
    12001 attcagattt aggtggattt aagggaatta ttgataaagt gggcagttca atggaatcgt
    12061 tcttaaaagt aattgctgca aatattccta cagcagtatc atttttaagt aatttattta
    12121 acgaaattca aaaatttggt tctgctttga aattcatgat gccctttctt gttcctgcag
    12181 ctaccgcttt tggagcgttc atgtttcaac ttaaaggtat accagcaata ataaaaagct
    12241 tcaataattt taagaatgcc ataattggcg ttggaaattc attaaagatt atgggggcaa
    12301 tagctgctgc taattcattt gttttaatta ttggtgcagt cgttggtgca atagctgttt
    12361 tcggttactt tatggcaacc aatgaagagt ttagaaacaa ggttatatct atttggaatg
    12421 atgtgaaaga ttccgtaatt ggtgtattaa aaaatataaa ggactgggga attgatactt
    12481 ggaattctgc taaagaaatg gcatcaaatg cagttgaggg tgtcaaagat gcttggtcag
    12541 gaacaaagga atggttttct aacacctgga aaggtattaa ggatggggcc acaggtttat
    12601 ttgataaaac agtagagact tcaagaaatg cagtcgatag tgtaaaaaat gcatggtcta
    12661 atatgaagaa atggttttct gatacttggc aaagtataaa agattcagcc agagaaaaat
    12721 ggaatgaaat taaaggttct attatgtcgg ttgctggtcc attgattaca ggtataaaga
    12781 atgcattttt acatgttact ttttatctag agaccttatg gaataattta gttgagattg
    12841 gtaaaaacgt ctttgagatt ttaaaaaatg ttatacttgc gccggttttg tttattacct
    12901 ccatgatttc tggtggatgg gaagaaacaa aaaataatat gattgctgtt tggaataata
    12961 ttaaagaaag tgccataaat atttgggaat ctataaagaa tatatttgta agttatttta
    13021 caaatattta ttttgctgca ctcaatattt ggacagggtt caagcttaca ttgataaata
    13081 tttggaatga agtggtaaat caagctaaat cgatttggtt aaacataaag tattttttta
    13141 ttaatctttg gattgatata aaatattttg caattcagaa atggattgaa ttgaagtttt
    13201 ctattattca aacttggatc gatttaaaat ataatgccat tactacttgg aataatatta
    13261 aacagttttt caaagatact tggaaaaata ttaaagatac ggcatacaat acgtggattt
    13321 ctataaaaaa taccatgatt aatacatgga ataacattaa ggactctttc tggaatattg
    13381 ttactggaat tgttaattct gctgaaaatg catggacaaa tctaaaaaat ggtgtttcaa
    13441 atgctattaa tcgggtaaaa gaaatcttta attcgttaag ggaagtcaat ttatttgaaa
    13501 ttggcaagaa catcattgat ggacttataa atggtgtaaa agaaaagtgg aatgctttga
    13561 aagatactat caaaggtatt gcaggtaata tcaaaaattg gattaaagga gctttagata
    13621 ttcattctcc atcaagatgg atgcgtgata tggttggtaa aaatattgtt caaggtatta
    13681 tcgttggtat tgataaagag caaagtaagt tagatcaaac catgacaaat ttagtaaaaa
    13741 caccgtctgt tcaacctgta gtaaatggaa taagtcacct accaattaca caaacaaagc
    13801 aaaatatatc tgctagtgaa acaaaagaaa ttcatttaca tttaaatgtt tatggagatt
    13861 tacctgattc aatggttcgg aagattacca caaaaattaa aacagaattg acaagacaaa
    13921 tgaaacgaga tgctggtgca gtaggaggaa cattatatgc aacttaagag aggacaattt
    13981 tttattaatc aacattattc ttctgaattt aatgtgtata ttcaaaacag gcctgcttct
    14041 gtttcagcta gtcgagtaat tgaattgaga gagcgagaag gaaatgattc tatcattatt
    14101 gataaaggct attacagaaa tgtgactaga aagattgagt gttactataa agcaccttca
    14161 attgatgtgg tgcaggagtg ggaagatcga atcactgaat ggttagatat gtcgtcttat
    14221 agtgatttta ttctttacta tgatgaacaa tatatttatc aagctgtagt aatagaagga
    14281 cctgaattta aaggaacaag aaaaacagga aatattgtcc cttttgaatt tacagtgagc
    14341 attcgaccat ttaaggaaaa ttacaatggt agatttacta ttcgacaaac agaaactttt
    14401 gaaatttata acccagagaa gtattcttca aaaccgctta ttaaattgag tggttctgga
    14461 gatgcttctt tttatattaa taaggacaaa tatgatttga aatcgttgga cagagaatta
    14521 tttatagatt ctaaattaga agaggcttac cgaaaattag atggtaattt agaacatcag
    14581 gatcaagtca ctttattttt agattttcca tttttatatc ctggaaaaaa tgaaattaaa
    14641 tggacgaaaa atattcattc atttgaaata atgcctaggt ggtggagaaa agtatgaaac
    14701 caagaatata tagtcctact gaaacagatt tctcaacgaa tggcttagga attttaaagg
    14761 attgtacaag atgtgaaata tatgaagtag ctaatggaaa atatgaatta gagttggatt
    14821 atcctttagg aactagattt gatgaatatt ttgaaaatga ctatcaaata aaagcaaagc
    14881 caaacgatca agaagagtat catatctttt ttattgatga taaagatata gatacttttt
    14941 taaatacagt aactatttat gctcagagtc gtacaaatcg acttggaaga cgggtagtca
    15001 ctcttgcgga agtagactct aaaactggtc aagaagcgat atcaattatt gaaaccaaaa
    15061 tggataaaaa atctgacata cgactttatt ctgatattac ggccgtttct agcacaatct
    15121 ttgaagcgag aaatgtttta aattgtattg ctggtgaaca aggatcattg cttcaatatt
    15181 ggggtggaga aattaaacgt gaaccattta agctttcttt gttaaagcga agaggtcgca
    15241 ataatgttgg aacgattcgg tatggaaaag atatgtctgg tttaaaggtc aaattagatt
    15301 ggacaggtat aaaaacaaga cttattccat atgctgatcc tcaaagtgag gtaggtacga
    15361 ctaatcgaat ttatggttcg ccagtagata gtgcctatat taataactat cctgatgtgt
    15421 atacagagca tgttcagttt acagaagaac aaggagtaaa agacattaag agcttaaata
    15481 aaatagctaa aaattacttt aaaacgatca atcctggctg tgataagcct aaagtttcta
    15541 ttacggttga atttgataag ttgactgata gtgaagaagc gaaagaattt gcgaagatta
    15601 gaaattatgg tctgtttgat acatttaaaa tatatcataa aaaatataat atttatcttg
    15661 aatcaaaggt tagtggacta caatacgatt ctttatctga aaaaactttg aaattagaag
    15721 caggagacat tcaagttgct ttttatcagc aacaagctgt aactattcag gataaattaa
    15781 aagattatgc aacgaataat tatatgagtg attttaatga ttatgtttct tcaatgatta
    15841 caggtcaagg cagtgcagga ggatatgtaa gtttatggcc aaaagaaaaa ccttccaaca
    15901 tctttattat ggataatcca gatttagaaa aggcaagaga agtactaaga atgaataaaa
    15961 atgggattgc tttttctaag aaaggatggc gggggccttt tacttcagct tggacattag
    16021 atagtatatt taatgccaat tttatacaaa ctggattaat caaagcagat atctttcaaa
    16081 attcttttaa caagactgga gatgtattga aattagttaa tggactactc caaatttgga
    16141 acaacaaaaa gaaaataatg gagttaacta aaaaaggaat ggaattttgg aattctaagg
    16201 gttcaattgg aacgattgga acaactgatt ctgctggtaa tccttttcct ggggcttcta
    16261 ctccaacccc tattgaagat aattctttag ttattcgtac aaatggagac ggcaaatata
    16321 ttttgatttc tcctaaagta ggaaaaggat tagttttatt aggaaatggt aaagcaattt
    16381 attttggaga cttagatgta caaggaaaac ttacagttaa aggaaaagaa ataattcctg
    16441 gacaaggtgg tggtccatcg ggaggtggag aatctactgg tgggtatcct agtgaattaa
    16501 aaacagatgc agaaaagaga gcttggagaa tttacgatat tttgtgtaat aacggattta
    16561 cgaaacaatc tgcatgtgga atattgggga acattcaaca agaaacagga ggaacttttg
    16621 atcctgatac tgttcaaata ggtggaccag catacggatt agttcagtgg gatggttctt
    16681 catatcctct tgttggtcca gcaacatggg atggaaaagt ttatgttcaa aacttattta
    16741 atgctgcagg tattaaagaa ccaataacga gcttagatgc acaagttcga ttgcttattt
    16801 ggacatttac aaatggacaa tggatgggcg tagtacaacc tacgacggtt gatggattta
    16861 aggcctgtac tgatccaaga caagcagcat atgcttttga acgaaactat gaacgtccgg
    16921 cagcgacaca tcctgaacgt caagattatg cagttaactg gtataacaaa tttaaagatt
    16981 taaaaccagg aggagctact ggagaagcag gactaaaaca tttagagtct ttgattggac
    17041 aacgaattgg caatggtcag tgttatggct tgtctgcaga atattcagga tatctgggtg
    17101 gttgtggcat gggtgctgga acaaaatatg gtttaactca tgtgatagga aatacttctg
    17161 cagcatctga tattggtatt gcctatgatt ggtctgctgt tggttggaaa gtgattcaaa
    17221 accctagata tgatcaatta gtagttggtg caattattaa ttgggcaaga ggtggacaag
    17281 tgggttcatg gtttgcagac ggaacttatg gacatactgg cgttatccga ggcttagcta
    17341 atggtcgtat gcaaacttat gaacaaaaca cagagttagg tatgatttgt ggaaagttag
    17401 atcgccagta ttatagtgca agtgcaattt cttccattgt cataccacca aaatagggag
    17461 gtgatttaat ggcaaagtgg aatgtcatat taagtacaac agaaccctat aattacgtgg
    17521 ggatgattca agttcgacaa ggcaataaaa acactgaagt tatggaagct acaattgtcg
    17581 aaaatggtct tccatatgat ttatcagaat gtaaggtata ttttgaatca gttgttggcg
    17641 gaaaatatcc agtccaatta gaaacaagaa ttgtggatgc taaaaaaggg aaaatcaaat
    17701 atatttttga taaatattcc atgcagtgtt tgcatcgaca aacagccaat ttcattatat
    17761 ttaaaggaga agacttgatt ggaacaactc aagacttctc ctattttgtc atcaatgctg
    17821 tttcaaaaac agaaggagaa atgggttctt attggcaatc aattgaagat ttaattgctg
    17881 atatgacaga ttttattaat gaaaataaag gcgattttac ggactggatg aatgaaagga
    17941 aagaagaatt tgatcgctgg agagaagaac aggagaatag tttccaagat tggagagaag
    18001 gacaagaatc agattatttg tcatggtttg aatcaatcaa ggatattttg aagtctatcg
    18061 atccaggtgg agtaatgtta gccgaattaa tggatgcacg tgttgatatt caaggagttc
    18121 gtcatgcgtc tatttcagag cgcttgctgg cagatatgga ttatctatat cagaaattac
    18181 gagcaacact tttcacgatt gaatacggtg agattgaagt gactgatatt ttgcaggatg
    18241 atctcttttc agataatcac gaagtcgaaa aagttggaac tgtagaattt ccaattgaag
    18301 aaggagcttt gattattgca actgttgatg atccaaaaca gaatgttttc actcttgaga
    18361 aagttggggt gatttgatgg ttaaagtaaa gagaatgatg gagactgatg aatctggtgt
    18421 taaacgtcaa tttatgccaa ttacacatgt ttctgcagtt ttaggcttag aaaaaatgat
    18481 atcaggacaa tcaaaagttc tttcggttaa tggtaaatat ggtgcagtca ttttaacgaa
    18541 agctgattta ggattagaga atgcaattac tgaactacct tatgcaagcg agacaagcga
    18601 tggtattctt actgctgaaa tgtttcaaaa gatcgtaaat ggagaaggtg gaacatatat
    18661 tttgccgatt gctactcctg aacaattagg aggtataaaa gttggggaac tcttagaagt
    18721 tactgaagaa ggtgttttat ctgcaactaa gcaaacagac ttcaatttct ctgaagaatt
    18781 aaagggaaaa ttagaatctc taaaaatatt aaaagcaggt gcaaatatat caattgcaga
    18841 agatggaaca attagttcta caggtggtag tgggactggt ggtgttaatc agtcatatgt
    18901 agatcaaaaa ttccaagaag ctgtaaatca agcagaaagt tacacaaacg aaagaattcc
    18961 aaattttact tttgaaaaaa tcggggaggt atagaaaatg acagatatcg taaaaataaa
    19021 acaaagcggt gtacaggttt atccacaaac tcattggaat gctatagaag gcaaaccaac
    19081 aacggtgaag ggagataaag gcgatcctgg ccaagcagca acaattacca taggaaccgt
    19141 ttctagtggt tcaaccgctt ctgttacgaa tgtaggaaca tcatctgcag ctagatttaa
    19201 ttttgtttta ccaaagggag ataaaggtga tcctggaata aatgccacaa ctacagctgt
    19261 agctacaact acagcaaatg ggttgatgtc gtccacagat aaaactaagt tggatggaat
    19321 tgcagctgga gcacagaaaa atccaggtaa tgctacaact acgacagcag gtttaatgtc
    19381 agcaaccgat aaagtgaaac tcgatggatt aaccaatatt acatttgaga aagtggggac
    19441 ggtttaatgg ctgatattgt gcaattaaaa gaagacggag ttgctaagta tttgaaaact
    19501 catgccaaag gaattgatgg tgtggagggt gttttagtaa aagccacagg taatgaaacg
    19561 gtcttaggaa cgaaaaattt taaagacgga ttgcaattca atggattgcc tgtgcaagca
    19621 ggtatgatcg agcgagctat cacactagct gatcgaagtg atacaacaaa cgtaactgat
    19681 gtgaatggaa aaataatcag aatcggaaac atcgtatttc ttacttttaa ttttaaatgt
    19741 ggcacatggc ccgaaggatc agaaactcgt tggattttaa aaatcccgga cggtttcaaa
    19801 cgtgatcaag gctatccagc gcaaaccgcg ctttcactag ttagaaatgc aagtcagcca
    19861 gcggatgcac gtgcattcat tgatcaaagt agcatcatac aagccaaatc aggttcaggt
    19921 agttcatata tttcaggtat gtggatcact caagatcctt ggccggctta acagattact
    19981 aattggagga agagtaaaat gaaaacaatt tataaagtat tgtatccaat gggatatgaa
    20041 gaacatcaag tagaagataa ctttccaaca tctttaccat ttgtcgaagt tccacctatt
    20101 ttgtttgaaa agaaggaaga tgaaacggat gaaggtttcg gaagaagaca acagtcgcaa
    20161 ttctttaact tcactgaaaa taagtgggaa gaagcagtta cacaagatta ttcaaaaaaa
    20221 ttagaactac tagaaaattt atctataggt ttacaggtag ataatgctgc attgaaaaaa
    20281 tcaaatgaag agttaacgga aaaagcagat tcgatggcgc agttgaacgc caaactgatg
    20341 cttaatgatt taacaattaa taaaaaaatt gaagcaatag aaaaacaaat tggaggtgct
    20401 tcataatgtt tagttttagc gatgtgaaaa tgatgtatga ttggggctgt tttactgacg
    20461 atcaagttcg acaattcgtt ccactatgca ttacagacga a
//
LOCUS       DACEXQ010000023        20280 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.023, whole genome shotgun sequence.
ACCESSION   DACEXQ010000023 DACEXQ010000000
VERSION     DACEXQ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.023"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..408
                     /locus_tag="IXH04_000696"
     CDS             <1..408
                     /locus_tag="IXH04_000696"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="catalyzes the formation of
                     N(1)-(5-phospho-D-ribosyl)glycinamide from
                     5-phospho-D-ribosylamine and glycine in purine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAQ1851328.1"
                     /translation="GDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVV
                     AAAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQ
                     KKIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            530..1444
                     /locus_tag="IXH04_000697"
     CDS             530..1444
                     /locus_tag="IXH04_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAQ1851329.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(1577..3142)
                     /locus_tag="IXH04_000698"
     CDS             complement(1577..3142)
                     /locus_tag="IXH04_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ1851330.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(3135..4586)
                     /locus_tag="IXH04_000699"
     CDS             complement(3135..4586)
                     /locus_tag="IXH04_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ1851331.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTIEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(4600..5616)
                     /locus_tag="IXH04_000700"
     CDS             complement(4600..5616)
                     /locus_tag="IXH04_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1851332.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            5836..6924
                     /locus_tag="IXH04_000701"
     CDS             5836..6924
                     /locus_tag="IXH04_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ1851333.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            6978..8504
                     /locus_tag="IXH04_000702"
     CDS             6978..8504
                     /locus_tag="IXH04_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAQ1851334.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            8506..9711
                     /locus_tag="IXH04_000703"
     CDS             8506..9711
                     /locus_tag="IXH04_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1851335.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            9968..10945
                     /gene="guaC"
                     /locus_tag="IXH04_000704"
     CDS             9968..10945
                     /gene="guaC"
                     /locus_tag="IXH04_000704"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAQ1851336.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            11209..12633
                     /locus_tag="IXH04_000705"
     CDS             11209..12633
                     /locus_tag="IXH04_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ1851337.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            13094..14203
                     /gene="pdhA"
                     /locus_tag="IXH04_000706"
     CDS             13094..14203
                     /gene="pdhA"
                     /locus_tag="IXH04_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAQ1851338.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            14206..15183
                     /locus_tag="IXH04_000707"
     CDS             14206..15183
                     /locus_tag="IXH04_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAQ1851339.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARAR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            15208..16851
                     /locus_tag="IXH04_000708"
     CDS             15208..16851
                     /locus_tag="IXH04_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HAQ1851340.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            16859..18265
                     /gene="lpdA"
                     /locus_tag="IXH04_000709"
     CDS             16859..18265
                     /gene="lpdA"
                     /locus_tag="IXH04_000709"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAQ1851341.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            18427..18702
                     /locus_tag="IXH04_000710"
     CDS             18427..18702
                     /locus_tag="IXH04_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAQ1851342.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            18708..19475
                     /locus_tag="IXH04_000711"
     CDS             18708..19475
                     /locus_tag="IXH04_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAQ1851343.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            19643..>20280
                     /locus_tag="IXH04_000712"
     CDS             19643..>20280
                     /locus_tag="IXH04_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ1851344.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQV"
BASE COUNT         6614 a         3493 c         4461 g         5712 t
ORIGIN      
        1 ggtgatcctg aaacgcaagt cgttctttct cggttgaaga gtgactttgc gcaagtaatc
       61 gatgatttat tagagaatcg tacagtagag ttaaagtggc agcaagaagg atacaatgta
      121 ggcgttgtgg ttgcagctgc cggttacccc gaagaatatg agacaggaat ggttctacca
      181 gattttctgg aagaagagct ttctgtttac tatgctggag tttcctcaaa taacggcaaa
      241 ctagttggag caggaggacg tctttattta gtagaagcat acggtgagac gatcaaagaa
      301 gcacagaaaa aaatttacac cgcgttaatg gataaaacga cagatggaac attttatcgt
      361 acagatattg gagaaaaagc tgccaatagc gaactgatcg tgaagtaaat cagctgcttc
      421 catttataaa aaatacaaca aacataatcg gaagcgccga ttttagtgaa gccttttcat
      481 aaagagtgtg gtacactttt gttgtgtata taaataagga ggagttcaga tgaaaaaaat
      541 tcaattaggt acttctgatc agaaagcagc tgcagtgatc ttaggctgta tgcgtatcaa
      601 caatgcggaa gatccagtaa aagtgattga gacagcttat gaaaacggca ttgatttttt
      661 tgaccatgca gatatttatg gaaacggtga atgtgaagaa atctttgcag atgctctagc
      721 aaaaacatca atcaaacgag aagatctttt tattcaaaca aaatgtggaa tcgttccagg
      781 tgtgatgttt gatttttcta aaaaacatat cattgaatct gtagaaaaaa gtttgacccg
      841 tctgaaaatg gattatgtgg atgctttact tcttcatcgt ccagatacat tggtagaacc
      901 agaggaagta gctgaagcgt ttgatgaatt ggaacggtca ggaaaagttc gttattttgg
      961 cgtcagcaat caaaaaccaa tgcaaatcga attgctgaaa aaatatgttc gtcaaccatt
     1021 agtagctaat cagctgcaat ttggcttgaa acatactgga atgatcgatc aagggattca
     1081 tgtcaatatg acagatgaag caagtatcga tcatgacggt agtgtacttg attattcccg
     1141 attgcatgat atgacgatcc aagcatggtc accataccag tatggatttt ttgaaggtgt
     1201 atttatcggg aacgaaaaat tccctgaatt aaaccaaaca ttagaagaac tagcagctaa
     1261 atatcaaacg acaccaactg gcttggcttc tgcttggatt ttacgccatc cagccaatat
     1321 gcaagtgatc gccggtacga tgaacgtagg gcgaatcaag gaaatcgctc aagcttcaga
     1381 aattaatatg tctcgggaag attggtacga attgtaccgt gctgctggaa acattttacc
     1441 ttaaagattg tcataagcag gtcaaaaggg ctataaatta aaaagacaga ataacttgaa
     1501 agctgttttc agcttcgaga tattctgtct ttttttattg caaatggaaa gagtatcatt
     1561 gtacggattt cgtttattag ttatttttgt ttttaaggaa aagctgtgtc cgatattctt
     1621 ttggagaaat cccttgtttt ttcttaaata aataactgaa atagttaggg tcattataac
     1681 caatttcaaa agcaatatct gaaatacgat agtctgattg ctgcaataat ttttttgcta
     1741 attcgattct ttgttctgtc agataatcga tgaatgtttg gttaaacgat tgagaaaaaa
     1801 tcgtactgaa atgagaggga cttaatgcaa cttgttgagc gacccaagtt aaggacatat
     1861 ctggatcggt aaaattttct ttgataaaat ccaatgcatg gttcaaaatt ggctgatatt
     1921 tgttccttga aggctgtgag ggcagagcag ttaagaaaga aatgaacata taaactgtcg
     1981 aacggtaaag cgagtcgtcg gcgctgattg ctaagcgttg actggtggat gtgcaatgaa
     2041 ccatttcttg cgggattttc ttttctttca ggcagacatg ctggagtagc ttagtcaaga
     2101 caaataaacg atataatcgt gttcgagttg gtgtgccttg tggtttcgtc aagtgttcga
     2161 tgtacttaga tacttcgttt ttcgataatg aagacaaatc aagtgagagg tcaaagtaaa
     2221 agacagaatc aactggaagt ttcgcttctt gaatcaaatc ctcatagctg ataatttgtt
     2281 ctttaaggga actggcggtt tcaagtatca tctgcgtgat ctcaaaagat gcaggtaatt
     2341 cgctgattct ttctgcgaca ggaccaatgg cgacagaaaa attatttttt tgtccctttt
     2401 ctaattcatg gaccaacgtt tgggctacct gataactggt ctcgatcaca ttttcttcaa
     2461 gagaatcaac tattaaaaat ttgatgaatc gagaggaaac agcagagaag atcacttgtt
     2521 gattctccct aaataaaaac ttcaaataat cactgagatg agcgtaatct tcaaagtctg
     2581 tactgacttg gtttgctgct aataatacgg tcattttttt ccctgctaaa gagcgattga
     2641 aagattcagc aagtttcaat gtttcactga tcgtaagtga gcctttgaac aaaccgttca
     2701 agaaatgatt ttttttcaat tcgaaaacaa gatcagaaga agtgtgcgtt tctttttgct
     2761 gcaaatctaa agcagttact gctttttcta ttgcttgttt caattcgcta cttttaatcg
     2821 gtttaagcag ataatcatct gcctgtacct ggattgctcc tttcatataa tcgaaatcgt
     2881 cgaatccact aataaatatg agacgagtcc aaggaagtag tttccttgct tcttttgaaa
     2941 aagtcaaccc gtccatgaaa ggcatttgga tatcagtcag aatgatgtcg ggctgtaagt
     3001 cgataattga agacagtgcc atctcaccat ctccagcttc gccgacataa gtgattggat
     3061 aataagaacc aaggacagtt atttgttccc gcaagttatt gcgaatgagg tattcgtctt
     3121 cgatgataaa aactttatac atgtttgttt cctccaattg ttttgggaga tagagggatc
     3181 atcagtgtga cagttgttcc ttctcggtat ttgctgtcaa tcgtgatagg tttttgatca
     3241 tttccgtaat aaagaaggag acgcttgttt acgttgtaaa gtccgtagcc gatactgaaa
     3301 tctgaatcga tccctttggc taactcttta cgtatttctg ccagtttttc tgcagggatg
     3361 ccaatcccgt tgtctataac tttgaattgg atcatctcat caattttgca tgtggtaata
     3421 cgaataaatc ctttgtgacg gacgtacttc gttccatggt atacagcatt ttcaatcaat
     3481 ggctgcaaga tcatttttaa aatagaaaca ggaaaaagtg tttctggtat atcgatctta
     3541 tagtcaagca tatggccgta tcttattttt aagatgacta gataatcatt gatatggcta
     3601 atttcttttt caagaggaat ccagtcttgt cctttgctta aagacgtacg aaaaaagccg
     3661 gaaagtgcat gggtcatagt cagagcttct tcaaagcagt cttgttcgat caaagaaata
     3721 atggcatcca aactgttata aataaaatgg ggagtaatct gagcttgcaa tacacgaact
     3781 tcactttgtg ctaaatggta ttgtttcaaa gcgttctccc gtaaagactg gtttagatct
     3841 tccgccattt tattcatgct agcggtaagt gtttgtaatt ccagtgtttc cggcagggtt
     3901 aaacgaaagc caaggttccc tgaagaaagt tgttctgaca tctcgatcag ctgattgatc
     3961 ggttcttgga cctttctctc taaaaagcga cgattctttt tcgcatagaa aaaaatggta
     4021 ctaagaactc caagttccac tattgttaat agaatgagcg agcggacaag tgtcgtattt
     4081 tttttgcttg ctaagtcgat ctccacacga acaaaatctt gtaagatatc tgacagcagt
     4141 tgagtgacag aatctacctg attgatgatc tcttcaatgg tatccataaa atcttttgcc
     4201 aagatattat tgatcagctc gttttgataa tcttccagtg tatcgatcgt acgtagcgat
     4261 acatctaacg tactaatttc tttaagtgta tttgtattat ttttgatctc tcgaatatca
     4321 ttcctaagtt cttctaggat attttcctca ttgaactcat ctacggttac ttggccagaa
     4381 atcaagtccc acatactttc caaaactttt tgatcaacct tcaaagcaat tgcatctgct
     4441 ttttggatat ttgcgatcgt ctgttgataa atcaaaagct gttgcgaata taacagtatg
     4501 ctcagtagca aagggataac agcaaataga agcatggctt tgtttgcttg ggcaaaaatc
     4561 tgttttatgc ttaaattttt tgtcatgata cttctccagt caataatttc tggttgggat
     4621 gttagctata ttctgctggg aaaagacagt ctcggaaata aaaatatctt taggtaatgt
     4681 ttttcctgtt tgaatatttc caaaataacg agtaacggta ttggctacat accagccgac
     4741 atcaggttta cactcgacta cacaattgac gattcctgag cgtagcttct cgatcatatc
     4801 ttcttgtcca tcaatagtta caatgatcaa atcttctcca ggtttgattg atgtttcttt
     4861 tatcgcagct aaagcaccgt gagtcatttc atcactatgg ctgtataata catcgatttc
     4921 ggataaatca tttgtttcta aatatcgttt catctggtct tttcctttca tccgaataaa
     4981 atcaccgaca attcggtgaa caatagtcat cggaggttta tggtcgatgg ttccatcttt
     5041 ttttagggtt ggtttacgat tgattgattc caaaaatcct tcggagcgta gcttggtcgg
     5101 tgtggagttc tctgaaccag caagttctag gattctgatt tcctgtttct ttgaatttgt
     5161 gaagtgattg ctgacaaata aacccgcccg atttccttct gctttgaaac tggggccaat
     5221 gtgggtgacg aaaagctcgg gatcattcgt ttgaatatgt ctgtcgacaa gtatgacagg
     5281 gatccctgct cgctttgctt cttttaatac agagtcccac ccggattctt gcaaaggagt
     5341 aaaaatgatc atatccactt tataggcaat gaacgtccgt atatcttgga tttgccgttc
     5401 ctgattcatg tagccatttc ggtagagaac ttggtaatct tctttttcaa gcgcagtgat
     5461 aattgattca gtatgctttt tccgccagct actctccgtt cctgattgcg aaaaaccaac
     5521 gactagtggg tcaggtgctt cctttgtatt aggtgtacac cccatcattt ggtaagcgat
     5581 tacaacaagg gctaaaaaaa ataaaacacg tttcacttta aatcactcca tctggatttg
     5641 ttagtactat cttatcagat aaaagggaaa atcaaagtca aaaggaatca atcgaaaaaa
     5701 atccaagaaa cttaaaaaaa tccaagacaa aaaaagtaca cctaaaaaat ttaaagtaaa
     5761 tcccataaaa aaatcaatgt taacgctttc tttattagct atactcggtg tatcaattga
     5821 aatgggggat aaaaaatgaa aaacaaattt agaaagattg ttgggactat cgctattttg
     5881 accgcaagca ttctattaag tgcgtgtggc aattcaggat ctgcagatga tagtacgggg
     5941 tatgtaggaa tcgctatgcc gaccaaatca gcagaaagat ggatcgctga tgggaacaac
     6001 atggtctcgg aattagaaaa actgggttat aaaacagatc ttcaatatgg agaagataaa
     6061 gtagaaaatc aagtagcaca aatagaaaat atgattacaa aaggggtaga tacacttgtg
     6121 attgcttcta tcgatggttc ggctttaact gatgtgttag ctaaagcaaa agaagcagat
     6181 atcaaggtga tcgcctatga tcgtttactg atgaattcag aaaatgtcga ttattacgcg
     6241 acttttgata attttggagt aggtgtatcg caagcagctt atattgaaga gcacttagga
     6301 ttgaaagaag gaaaaggacc attcacgatt gaattatttg gtggctcgcc tgacgacaac
     6361 aacgcactaa tcaattacaa cggagtgatg tctgttttac aaccatatat ggacaacggt
     6421 cagttggtcg tgccatctgg acagacgagt ttcagtcaaa tcgctacgct tcgctgggat
     6481 ggttctacgg cgcaagcaag gatggataat ctgttaagcg caaattatac tgaccaaaca
     6541 ttggatgctg ttttgtcgcc atatgatcca ataagtttag gaatcatttc gtcattaaaa
     6601 ggcgtggggt atgggagtga aagtaaacct ttacctgtga ttactggaca agatgcgaca
     6661 gttgctggcg ttaaatcgat cattgccggg gaacaaacac aaacgatctt taaagacaca
     6721 cgtatcttag caaaaaatac gattgaaatg atcaaagcaa tcagtgatgg agaagaggtt
     6781 ccggtcaatg atactgaaac ttatgataat ggggtgaaaa ctgtccctac gtatttagca
     6841 aatacagtat ctgtcgataa agataattat caagcagagc tgatcgacac cgattattat
     6901 aaagaatccg atctgaaaaa ttaagaggaa ccaaacaagg aactaaagag atctaagtca
     6961 gagagaggat aagaattatg gcggattgta ttttaaaaat ggcagatatc accaaaaaat
     7021 tttctggaat ccgagcgtta gattgcgtga atctgatgat tgaacgtggg gaaatccatg
     7081 cattgtgcgg agaaaacgga gcgggaaaat caacattgat gaatgtcttg agcggcttgt
     7141 atccatatgg ctcctatgac ggggaaatct tttatgatgg aaagctgtgt caatttaaag
     7201 atttgaaaga tagtgaagaa aaaggcatcg tcatcattca tcaagaatta gcactaagtc
     7261 cttatctttc aataaaagag aatatttttc tcggaaatga acaagcagaa agaggcgtga
     7321 ttgattggga actgacagaa aagaaaacat atgaactgct gaataaagtc ggtttgtcta
     7381 tcgacccgaa tcttctagta tcccagattg gcgtagggca acagcaacta gttgaaatag
     7441 ctaaagcatt atcaaaatcc gtccgtctgt tgattttgga tgagcctacc gctgcattga
     7501 atgaagaaga aagcgcgaat ttgctgagat tgatccgaga gttgaaagag caaggtgtaa
     7561 cctccatcat tatttcacat aagctgaatg aaatcgtgga tatcgcagat cgaatcacca
     7621 ttttacggga tggacagacg attgagacac tagaaaatga aatgatcagc gaagagcata
     7681 ttattcgcgg aatggtgggc agagatttag ttgatcgtta tcctgaaaga aaaccggata
     7741 ttggagaagt ttattttaaa gtagaagact ggacggtcta tcaccctctt gatcacgagc
     7801 gaatcataaa cgaccacatc aacttgcatt tgaaaaaagg agagattgta ggaatagctg
     7861 gactgatggg agcgggcaga actgaattcg ctatgagtct atttggacat tcttatggaa
     7921 gcaagatatc cggacgtata ttaaaggaag gggcagagat atcagttaag accgttcctt
     7981 cagccattgc aaacggactc gcttatgtct cggaggaccg aaaatcttta ggactgaatc
     8041 tgctgatgga tattcgggaa aacgtgacgc tgagcagttt ggataaaata agtcaaaaag
     8101 gagtattaga taaagaaaaa gaagtaactg aggcagaaaa tttccggaaa aagatgcgta
     8161 taaaaacaaa ctccatccat caaattgtca gtagtcttag tggtgggaat caacaaaaag
     8221 tcgttttatc caagtggctg atgacacaac ccgatattct ttttttggat gagccaaccc
     8281 gtgggataga tgttggggca aaatatgaga tctacacgat tatcgaagaa atggcagcaa
     8341 atggcaaagc tgtctgtctt atctcttctg aacttcctga aatattaggg atgtgtgatc
     8401 gaatctatac gatgaacgaa ggacgtatta caggagaagt ctcgcgagaa gatgcagatc
     8461 aggaagtctt gatgaaatta atgacgaaag aagaggtggg ttaagttgga acaggcaaaa
     8521 aatcttacgg ataaaaaaga gaaaaaacta aatttgaaaa gtagagcagc tgatcttttt
     8581 ggtcgctaca gcatggtgat cattttagcg ggactactta ttgcgtttca attgatgacg
     8641 gatggtattt tctggcgtcc attgaatata actaaccttg ttctgcaaaa tagccatata
     8701 ttagtactag ctgcagggat gttattagtt gtgctcttag gtcatgtgga tttgtcggta
     8761 gggtcagtga tggcgtttgt tggtgcaatt gcgggcatga tgatggtcaa tcgccaaatg
     8821 aacccgtgga tgacagttat cgcatgtctt gcagtaggcg ggctgatcgg cgcatggcaa
     8881 ggtttttggg tagcttatgc agggataccg gcgtttatcg tgactttagc aggattgctg
     8941 atgtttcgtg gactgacaca agttgttcta ggaggccaat ctctagcacc ttttccagct
     9001 gttttccaaa aaatgtctac gggttattta ccagacttaa gcgaaggaag cagccttcac
     9061 ttcttgacat tgattttagg aatgatcatc acgatcgtgt tagttgttgg tcaatggcga
     9121 acaagaaatc gtcggaaaaa tcatttattt gaagtggaat ctccaacagc tttcttggta
     9181 aaggcggtat ttacagcagt cgttgtacta ggaataactt atatctttgc ctcttatcaa
     9241 gggtatccga ttattttgat cattcttggt ttgatcgttg cagcatatgg gtttttgaca
     9301 aataaaacag tagcaggtcg acagatttat gcgacgggcg gaaatcgaaa agcggctgaa
     9361 ctatcaggga tcaaaacaaa gcgaatcacg ttctgggtat tcgtcaacat gggagtcatg
     9421 gcagctttag ctggattagt tttagctgct cgattgaatg cggcaacacc tcaagcaggt
     9481 acatcgatgg aactggatgc aatggcggca gtatattttg gtggtgcttc gacttccggc
     9541 ggcattggaa caatcatggg tgcgattgtc ggtggcttgg tcatgggtgt attgaacaat
     9601 ggcatgtcga ttttaggcgt cggggtagac tggcagcagg caatcaaagg attgatcttg
     9661 cttttagccg ttgtgctaga cttgtataat aagaaaaaac gaacaacgta ggagaacgcc
     9721 ggttattcag aaaaaagact gtgacaagac atttgtcaca gtcttttttt cgctaaaagc
     9781 gaacattcat tgtttcgttt gtatagacta aaaataatat atgttttttt accattcgta
     9841 taaattctct ttttgctctt gatttttttg cttaattgag ttaaattaca tagggcgcta
     9901 aaaaaagcct tgattaaatt aataatgttc gtgttttgct ggatgtgaac atcaggagga
     9961 taatgtaatg aaagtatttg attacgaaga tattcaatta attcctaata aatgtatcgt
    10021 aaatagccgt tctgaatgtg atacaacggt cacattaggt aaacatacat tcaaaatgcc
    10081 agtagtgcct gccaacatgc agacgattat tgatgaaaca atcgcagaat tcttagcgga
    10141 aaatggttat ttctatatca tgcatcggtt tgatgaagca gctcgaattc cgtttatcaa
    10201 aaaaatgaaa aagcgtggat tgattacctc gatcagtgtt ggcgtcaaga aagaggaata
    10261 ttcctttata gagaagttgg cagaagaatc gctaaatcct gattatatca ctatcgacat
    10321 cgcgcatgga catgctaact cagttattga tatgattcag catattaaaa aatatttgcc
    10381 tgaaactttt gtgattgccg gtaatgttgg tacaccggaa gccgtccgtg aattggaaaa
    10441 tgcgggcgca gatgcaacaa aagtcgggat tggtccagga aaagtatgta ttacaaaaat
    10501 caagacaggc tttgggactg gaggctggca actggcagct cttagatggt gtgcgaaagc
    10561 agctcgcaag ccgatcatcg ctgacggtgg gatcagaaca catggcgata ttgcaaaatc
    10621 tgtccgtttt ggtgcgacaa tggtcatgat tggttcattg tttgccggtc acgaggaatc
    10681 tcctggcgaa acaaaagtcg aaaatggaat cgtgtataaa gaatactttg gttctgcttc
    10741 agaattccaa aaaggcgaaa aacgaaatgt tgaaggaaag aaaatctgga tccaacacaa
    10801 aggatctttg aaagatacac tagtggaaat gcaacaagac cttcaatcat ctatttctta
    10861 tgctggagga agagatctgg aagccattcg aaaagttgac tatgtcatcg tgaaaaattc
    10921 gatttttaat ggagacacaa tctaaaaaat aaataaaata ttaggatgtt gtgaaaaagt
    10981 tgtcctgcat taagtaataa gaacagccaa acaaaaatag ttctttatat gttttgtttg
    11041 gctggaatta ttatcgcaga tacaagcttt tgaacatcat tattcagaat aagggactgt
    11101 gataagattt ttgtcacagt cccttatgtt ttgcaaaaaa agtttttctt ttttctcgta
    11161 aattcggtaa aatcaaaaga aaaaatagac aatagaaaag agaagcttat gtgggaaaaa
    11221 gtagagaaaa aacaaggacc gatttatcgt caaattatgg aacagattat gagagaaatc
    11281 gaaaacggaa ggatcaatcc tggaagtcaa cttgaatcag aacgaaaact agggcaactt
    11341 tttggtgtga atcgttccac cgttgtccat gcattggaag agcttaggga gcttggggta
    11401 ctaacgagca aacgtggcag cggatggttt gttaatcaga ctgaatgggg gaaattcgct
    11461 gttccaagaa tcgattggcg gcaaatgatc tctcctcgtt atgagcagac ggactggtat
    11521 gagcaaaaaa taagagaagc aaagcagatc caaaaaacat cgtttcttga tttatacgct
    11581 agcgagatgc cagatgagct tttgccgaat tttgagtttc cttcttattc tcttgaagaa
    11641 atcttagcgg aagaaaaaga aatggatctt ttaggctata ctccgttacg taacaaaatc
    11701 aaacggtatt tggaaagtaa atttgaattg gcattttccc ccaaccagct tcttgtgact
    11761 ggtggcggtc agcaagcaat ctttttgatt ttacaaacga ttctttcagc tggagagggg
    11821 atagctgtag agtcaccttc ttttttttac cgattacctc ttttcaaagc ttcaggaacg
    11881 cgtttatttg ggataccgat ggatgaagaa gggatcgact tagaaaaact gaaagaatcg
    11941 atccataaaa ataaattgaa agcagtgctt atcaacccga attttcaaaa ccctacagga
    12001 aaagtgatga gtcaaaaacg acgagaaaag ctagtagaat tgtgtcgaca gtatcggctt
    12061 ccaattattg aagacgatgt atttacggat ctttcatttc taaaccaaga agaagtcctt
    12121 cctatacgaa cgattgatcc ggataatgtg ttatacgtcg gttcgttatc tagggtttta
    12181 ggaaaaacaa cgaaaatcgg ttgggtgata ggacctgaga cttttatctc gcaactagct
    12241 caagcgcaag aaatgatgga gttttcgatg aatatcctta cacaaatcac agtggctaat
    12301 gtatttaatg aagcaattga tttgaaaatg caagatgtaa gaaaaaagtt gaaagaaaaa
    12361 agccgactga taagcgcttg gggagattca caatcgtttt tcaagcttca ttcaatttta
    12421 ggtggttact atgcctggat tacttgggat gggaaaaaac tagaccccga actagcagaa
    12481 aaagtagtag atatgggtct tggaattgca ccaagtctac tttttgggca agatataaat
    12541 ggaatcagaa tcaattacag taggctggat gaaaaccaaa taccgttgtt ttattcaaag
    12601 atgaaaattc tagaaaagtg gttaacagaa taatttcaca aagtaaatag attaaagaat
    12661 ataacagctt ggatttaaat ttcaagctgt ttcttttttt tcggaaaaag aatacgatat
    12721 aaagaaacta agaaaaaacc attatttgta cgtccgttca cgatgtgtaa ataaaataaa
    12781 tgtttgcaac aaaactgtat aacaccaacg tttttaaata gaaacagatg gttgtaagtt
    12841 tgattagaaa atagaaaaaa tgcagatgaa atataaaacg ctttccttat tgtgatttta
    12901 ttcactaatg ctgttaatat gcggattggc ataatttcct ttcatcgtga aaatttgtgc
    12961 ttttagcttg tatttctcat aaatccatga taatttatta atgaagacgg ttaaatagtg
    13021 atacagtttt attcactgtt tcaatcgcag aatgaagaga ttttcttata gatgagagag
    13081 gaatgtgtga caaatggcaa acaaaaaagc accgatcgac ttccaagcac tgctagatga
    13141 agtcaatgca gatttcccag tatatcaagc acttgatcaa gatggaaacg tagttaacaa
    13201 agatttagta cctgatttat ccgatgatga actcgttgaa ttaatgactc atatggtatg
    13261 gtctcgtgta ttagaccaac gttcaacagc gctgaaccgc caaggacgcc taggcttttt
    13321 tgcaccaact gctggacaag aggcaagtca gctggctagt gcttacgcat ttgataaaga
    13381 agatgttctt cttccaggtt atcgggatgt acctcaacta gtaaaacatg gtttaccttt
    13441 atctcaagct tttttatggt ctcgaggaca tgctgcaggt aattcttatc cggaagaatt
    13501 gaaagctcta ccaccacaaa ttatcatcgg tgcacaatat gtacaagcag ccggtgttgc
    13561 attaggtttg aagaaacgaa acaagaaaaa tgtcgtcttc acttatactg gtgatggcgg
    13621 ttcatcacaa ggtgattttt acgaagcaat caatttcgct ggcgcttacc atgcaaatgc
    13681 cgtattttat atccaaaaca acgggtatgc gatctctaca cctcgtgaaa aacaaactgc
    13741 agcaaaaaca cttgcacaaa aagctgtcgc agctgggatt cctggtattc aagtcgatgg
    13801 aatggatccg cttgcagtat atactgtatc taaaatggca cgggaatggg cgatcagtgg
    13861 aaatggacca gtcctaatcg aaacactgac ttatcgttat ggaccacata cactatctgg
    13921 tgacgatcca acccgctacc gtacaaaaga cacagatgac gattggcaga aaaaagatcc
    13981 attgatccgc ttccgtaaat atttgacgga aaaaggactt tggtctgaag aaaaagaaga
    14041 acaagtcatc gaagcaacta aagaagaaat caaagcagct attgctgaag ccgacaaagt
    14101 accaaaacaa aaggtttcag atttcttgaa aaacatgttc gaagtatctc cacaatcaat
    14161 caaagaacaa attgcaattt atgaagcgaa ggagtcgaaa taatcatggc acaaaaaaca
    14221 atgatccaag caatcacaga tgccttagca cttgaactag aaaatgacga gaatgtcgtt
    14281 gtttttggtg aagatgttgg gaaaaacgga ggagttttcc gggcaactga aggattgcag
    14341 gaaaagttcg gagaagaccg agtattcgat actcctttag ctgaatctgg aatcgcagga
    14401 ttgagtttcg gtcttgcgtt agaaggattt cgtccagttc ctgaaatcca attctttggt
    14461 tttattttcg aagcaatgga cgaagtagta gctcaaatgg ctcgtgcaag atatcgaatg
    14521 agcggcacta gaaatctacc catcacaatt cgttcgccat ttggtggcgg tgttcataca
    14581 ccagaattgc attcggataa tttagaaggt ttgatcgctc agtctcctgg tattcgagta
    14641 gtgattcctt ccaaccctta tgatgcaaaa ggactattga tttcttctat tagaagtaac
    14701 gatccggttg ttttcctaga acacatgaaa ctatatcgct cattccgaga agaagtgccg
    14761 gatgaagctt acgaagttcc tttggataaa gcagcagtta ctcgtgaggg aacagatgta
    14821 tctattatca catacggagc aatggtgcgt gaagcaatca aagcagcaga caatcttgca
    14881 aaagaaaata tatctgttga aatcattgat cttcgtacag ttgcgccatt agatgtagaa
    14941 acgatcattc aatccgtgga aaaaactggt cgcgtagttg ttgtccaaga agcacaacga
    15001 caagcgggcg ttgccgcgca agtagtttct gagatctcag aacgcgctat cctttcatta
    15061 gaagccccaa tcggacgagt ttctgcacct gatacagtct tcccatttgg acaagcagaa
    15121 aatgtctggt tgccaaatgc aaaagatatc gaagacaaag taaaagaaat cgcggaattt
    15181 taagaaaaga agggacgaaa caaagtcatg gcttatcaat ttaaattgcc ggatatcggt
    15241 gaaggaatcg cagaaggtga aatcgtcaaa tggttcgtca aaccaggaga tacgatcaat
    15301 gaagatgata cattactaga agttcaaaat gacaaatcag tagaagaaat cccgtcacct
    15361 gttacaggaa cggttaaaaa tgtgattgtt ccagaaggta ccgtagcaaa tgttggggat
    15421 gtattagtag aaatcgatgc accaggacac gaagacaatg aaggggacag tggtgtagcc
    15481 gcagaatccc aaactccagc taaaccggct gctgaaccca ctgttgatac agaatcagct
    15541 ggatcgtcaa gcgaaggagt cttccaattt aagttaccgg atatcggtga aggaatcgca
    15601 gaaggtgaaa tcgtcaaatg gttcgtcaaa ccaggagata cgatcaatga agatgataca
    15661 ttactagaag ttcaaaatga caaatcagta gaagaaattc cgtcacctgt cacagggaca
    15721 gttaaaaacg tgattgttcc agaaggcact gtagcaaatg ttggggatgt attagtagaa
    15781 atcgatgctc ctggacacaa tagtgcaccg tcaactagtg cgccatcagc tgaagcacca
    15841 aaagaaaaag tggaaacatc aggttcagca agtgtcgttg aagctgccga tccaaataaa
    15901 cgtgtattgg ctatgccatc tgttcgtcaa tttgcccgtg aaaaagatgt agatatctca
    15961 caagtaactg caactggaaa aggtggcaga gtaacaaaag aggacatcga aaacttcttg
    16021 gcaggtggtc caagcagtgc accagcgaaa tcagaagcgc cagaagcggc agcaccgaaa
    16081 gaagcagctc ctgcagcaga aagcaaacca gctgcaccag caaaaccatt caagtccaac
    16141 ttaggagact tggaagaacg tgtagcaatg acgccaacac gtaaagctat cgctaaagca
    16201 atggtcaaca gtaagcatac agctccacac gtgacattgc atgatgaagt agaagtgtct
    16261 aaactatggg ataatcggaa acgatttaaa gaagttgctg ctgcaaatgg tacgaaactt
    16321 actttcttgc catatgtcgt gaaagcttta acagcgactg ttaagaaata cccagtattg
    16381 aatgcatcta tcgacgatgc aaaccaagaa atcgtttata aacattacta caatatcggt
    16441 attgcaactg atacggatca cggactatat gtaccaaatg tgaaagatgc agaccgcaaa
    16501 ggaatgtttg ctattgcaga cgaaatcaat gagaaagcaa aattagctca tgacggcaaa
    16561 ctatctgcag aagatatgcg caatggaaca attacgatca gcaatatcgg atctgtaggt
    16621 ggcggttggt ttacaccagt catcaactat ccagaagtag ctattttagg tgtaggaacg
    16681 attgcacaac aaccaatcgt aaatgctgaa ggcgaaatcg ttgtcggtcg tgtgatgaaa
    16741 ctgtcattaa gtttcgatca ccgtatcgta gatggagcaa ctgcgcaaca agcaatgaat
    16801 aatatcaaac gtcttttagc tgatccagag ctattaatga tggaaggatg ataaacaaat
    16861 ggtagttgga gattttgctg tagaattaga tacagttgta attggtgcgg gacctggtgg
    16921 ctatgtagct gccatccgcg ctgctgaaat gggacaaaaa gtagcaatca ttgaaagaga
    16981 gtatattggc ggtgtttgtt tgaatgtcgg ctgtattcct tcaaaagcac tgatagctgc
    17041 aggacaccat tatcaagagt cactagattc tgaaatgttt ggagtaacta gtgaaaacgt
    17101 caaactagat tttgcaaaaa cacaagaatg gaaagaaaac aaagttgtcc atacgttgac
    17161 ttctggtgtt ggtttcctat taaaaaaaca taaggttgaa acaatcgaag gagaagcgtt
    17221 cttcgtagat gatcacacac tacgcgtcat tcatccagat tcagctcaaa cttattcgtt
    17281 caataacgca atcatcgcaa caggttcccg tccaatcgag attccaggat ttaaattcgg
    17341 aggacgtgtg ttagattcta ctggcggatt agcattgaaa gaagtaccta agaaatttgt
    17401 gatcatcggt ggcggtgtga ttggtgccga attgggcgga gcgtacgcaa acttaggagc
    17461 agaagtaacg attcttgaag gctcaccaca aattttacca acttacgaaa aagatctagt
    17521 aaaattagta gaagatgatt tcaagaaaaa aggcgtaaca gtcgtaacga atgcaatggc
    17581 gaaagaagca gtcgataacg gtgacagcgt gacagtgaaa tatgcagtgg acgggaaaga
    17641 agaatccgtt actgctgatt atgtgatggt cactgtaggt cgtcgtccga atacagatga
    17701 tatgggctta gaacaagcgg gtgtcgaagt aggagaacga ggattgatca cggtggataa
    17761 acaaggacgt acgaacgtac ctaacatcta tgccatcggt gatatcgttc caggggcggc
    17821 attagcacac aaagccagtt atgaagcaaa aatcgctgct gaagcaatct ctggcaaaaa
    17881 agtagctgta gattataaag caatgccggc agtagccttc actgatcctg aattggcttc
    17941 tgtaggtatg acgatcaaag aagcaaaaga tgctggtatc gaagcaaaag catataaatt
    18001 cccattttca gggaacggtc gggcattatc gcttggtaaa acagaaggat tcatccgtct
    18061 agttacgacg atcgaagata acgtgcttat tggcgcgcaa atcggtggag tcggcgcaag
    18121 tgatatggtc tctgaattag ctttagcaat cgaatcagga atgaatgctg aagatatcgc
    18181 cttgacgatt catcctcatc catctcttgg cgaaatcgtc atggatgctt cggaattagc
    18241 attaggttta ccaatccata tttgatttga ataatacagg cgaaacgcaa ctgaaaaatc
    18301 agttgcgttt tgttttttag aattatcaat aatattatta tgtaaactta aaaatatgac
    18361 aacttcttgg tttattacca cttcctatgc tacactgaag aggaatatga aataggagag
    18421 ttgaagatgc aagaatatca gtatccactt gatttagatt ggacaacaga cgaaatggtc
    18481 attgtaatga agatgtggga agatctggaa cgtgcgaatg aaaaagggat aaacaatgaa
    18541 gcttttttga agacttatca gcagtttaaa actgttgtca aatccattgg tgaggaaaaa
    18601 cgattgggac gggaatttga aaaagcttcc ggttattctt tatatcgtac agtgcagcaa
    18661 gcaaaaaaaa atccaaacaa aactttgaga atgaacgggt gagataaatg gaacagatga
    18721 ttgatcatat caagtcctgg cttgaagaag ctggtgagaa aatcaaatct tctcaacaaa
    18781 cattaacaat ctcgcaaaaa tcaaatcgaa cggatctagt aacaaatgtc gatcgaaaca
    18841 tccagaattt tctaatcgaa aaaatccatt cctttgatcc agaggcgaaa atattggcag
    18901 aagaagaagg gtgtaatcaa ttatcggatc tcgatggaag agtctttatc atcgatccta
    18961 ttgatggcac cttgaatttt gtgattgaag gggaaaattt ttgtattatg ctcgctgttt
    19021 atgaatccgg cgttggaaag cttggtttca tttatgacgt gatgagaaat gaactttttt
    19081 ggggtggaaa aggaataggc gtgtataaaa atgatgagcc attgcctaga ccagcaaata
    19141 aatccttatc ggaaggctta cttggtgtga atagttatct tttcggtcat aataaatatg
    19201 gaatccgaga aattgcggaa agcagcatgg ggacacgtat gtacggatgc gctggtcttg
    19261 aactagtggc aatcttgaaa ggaaaccaca ttgcttatat ttcaaacctt agcccttggg
    19321 attatgcagc gggaaatgtc ttattggaag agtttggtat ggtaacaagc ggtctttttg
    19381 gtactccttt gacatttgaa aaaagggaat actatttagc agggacttct tgtgcatatg
    19441 cagaaatcgc agaccttttg aaaaaagaag gctgaaaaaa aggtagaaat cattcacaaa
    19501 aatgataaaa acgagcaaaa gattaatttt ctcgaaaaaa tgaaaacatt ttactatatt
    19561 tcatgaaaaa agtctgctat aattagcagt cgagtaaaat ttgccctgtc ataaagaacc
    19621 aataaaaaag gagttagtta cattgaatta tagaaacgat atccgtaacg tcgcaattat
    19681 tgcccacgtt gaccacggaa aaacaacatt agttgatgaa ctattaaaac aatcagatac
    19741 attagacgca catacgcaat tgcaagaacg tgcgatggac tcaaatgcgc tagaaaaaga
    19801 acgtggtatc acgattttag ccaaaaacac agctgtagac tataaaggta ttcgcgtaaa
    19861 catcatggat actccaggac acgcggactt cggtggcgaa gtagaacgga tcatgaaaat
    19921 ggttgacggc gttgtgttgg tcgtagatgc gtatgaagga acaatgcctc aaacacgttt
    19981 tgtattgaaa aaagcactgg aacaacatat cacaccaatc gttgtcgtaa acaagattga
    20041 caaaccttct gctcgtcctg agcatgtcgt agatgaagtt cttgaactat tcatcgaatt
    20101 aggagcagat gatgatcagt tagactttcc tgtgatttat gcatctgcac taaatggtac
    20161 gtcaagcttg tcagatgatc cagcagatca agaaccaacc atggcaccta tttttgatac
    20221 aattattgaa aaaatccctg ctcctgtcga taacagcgac gaaccattac aattccaagt
//
LOCUS       DACEXQ010000024        19661 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.024, whole genome shotgun sequence.
ACCESSION   DACEXQ010000024 DACEXQ010000000
VERSION     DACEXQ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19661
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.024"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            5..1510
                     /locus_tag="IXH04_000713"
     CDS             5..1510
                     /locus_tag="IXH04_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ1851345.1"
                     /translation="MLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSANKSG
                     FRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETNEVGI
                     HEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQESPFGI
                     KTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMPTFGD
                     WELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVKAKKH
                     SKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITLLKHA
                     DRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSANYAT
                     EDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSGFDTK
                     ATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            1500..2771
                     /locus_tag="IXH04_000714"
     CDS             1500..2771
                     /locus_tag="IXH04_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1851346.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            2946..3842
                     /locus_tag="IXH04_000715"
     CDS             2946..3842
                     /locus_tag="IXH04_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1851347.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            3856..4719
                     /locus_tag="IXH04_000716"
     CDS             3856..4719
                     /locus_tag="IXH04_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1851348.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            4725..5288
                     /locus_tag="IXH04_000717"
     CDS             4725..5288
                     /locus_tag="IXH04_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAQ1851349.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            5298..6821
                     /locus_tag="IXH04_000718"
     CDS             5298..6821
                     /locus_tag="IXH04_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ1851350.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            6833..7792
                     /locus_tag="IXH04_000719"
     CDS             6833..7792
                     /locus_tag="IXH04_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAQ1851351.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            7878..8957
                     /locus_tag="IXH04_000720"
     CDS             7878..8957
                     /locus_tag="IXH04_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1851352.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(9209..10609)
                     /locus_tag="IXH04_000721"
     CDS             complement(9209..10609)
                     /locus_tag="IXH04_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAQ1851353.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            10827..12488
                     /locus_tag="IXH04_000722"
     CDS             10827..12488
                     /locus_tag="IXH04_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAQ1851354.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            12445..13143
                     /locus_tag="IXH04_000723"
     CDS             12445..13143
                     /locus_tag="IXH04_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ1851355.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            13159..14583
                     /gene="araA"
                     /locus_tag="IXH04_000724"
     CDS             13159..14583
                     /gene="araA"
                     /locus_tag="IXH04_000724"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAQ1851356.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            14890..16049
                     /locus_tag="IXH04_000725"
                     /pseudo
     CDS             14890..16049
                     /locus_tag="IXH04_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
     gene            16167..18026
                     /locus_tag="IXH04_000726"
     CDS             16167..18026
                     /locus_tag="IXH04_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ1851357.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            18136..18570
                     /locus_tag="IXH04_000727"
     CDS             18136..18570
                     /locus_tag="IXH04_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851358.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(19009..>19661)
                     /locus_tag="IXH04_000728"
     CDS             complement(19009..>19661)
                     /locus_tag="IXH04_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAQ1851359.1"
                     /translation="FHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTDRVYTYDVS
                     ENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKETGAFEELQK
                     ISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQNKQIISTEG
                     DFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECVCVYFDR"
BASE COUNT         6458 a         3346 c         4189 g         5668 t
ORIGIN      
        1 cacaatgttg attagtagtg tcaagcgtgt aggcaaagtt gatccgcggt tgtacagttc
       61 atttattgaa catatgggac gagcagtata tgaagggatt tatcagccag atcatccatc
      121 agctaataaa agtggatttc gccaagacgt cattcacttg gtaaaagaat tgaacattcc
      181 catgattaga tatcctggtg gaaattttgt gtcaggattt cgttgggaag acagtattgg
      241 gcccatagca aatagaccta gaaggttgga tttagcttgg cggacaattg aaacgaatga
      301 agttgggatt catgaatttt ataactggtg tcagcatgtg ggggccgaaa tcaatatggc
      361 tgtcaatctg ggtactcgtg gagtagatgc agctcgtaat ttagtcgaat attgtaattt
      421 tcctaaagga acttattgga gtgatttaag gcgttcgaat ggtcaagaga gcccttttgg
      481 aattaagaca tggtgtttag ggaatgaaat ggacggtcct tggcagattg gacataaaac
      541 agcggaagaa tatggacggt tagcagagga aacggctaag gcgatgaaac tcgtagatga
      601 ttcaattgag gtggttttat gcggaagttc gaatcggcaa atgcctacat ttggtgattg
      661 ggagttaaca gttcttgatc aagcgtacga tcaaatagat tatttgtctc tgcatcaata
      721 ttatggcaac caaaaaaatg atctttctca ttatcttgca cgttccttag acatggatcg
      781 gttcatttct gaagtgacag cgatgtgcga tacagtcaag gcgaagaaac atagtaagaa
      841 gcagatcaac ctgtcctttg atgaatggaa tatttggtat cattcagcgg aacaagatga
      901 gcaagcagcg ccttggcaga ttgctcctcc tttactagaa gataactata attttgaaga
      961 tgcattacta ttaggctgtc tattaatcac tttattgaaa catgcagata gagtaaagat
     1021 tgcttgtctt gctcaattgg taaatgttat tgcaccaatc aggacagaaa caaacggaga
     1081 agcttggaga cagacgatct attatccatt tatgcaagct gctgtgtatg gacaaggaga
     1141 ggtgttaagc ccacaaattc gttcagcaaa ctatgcgacg gaagattttg aagaagtgcc
     1201 gtatttagaa agtatagccg taataaatga caaagaactt gtcatatttg cagttaaccg
     1261 tggagaagaa gaaatgaatc taactgttca cttgtctgaa ttatcattag agggagtgat
     1321 tgatttttct gaaatgagtg gatttgatac aaaagcaact aatcttccta aaagtgaaca
     1381 agtaaaacca agtcattcaa caaatgtaaa agctgaagat gggaaaatca attgtttatt
     1441 gaaaccgcta tcttggaatg tggtcagatg tcgtttatca gatgaaggag gaacaaaaaa
     1501 tgaaaggtaa aaaatggtta attggctctt tgttggtagc tgcaacaggt attttaggag
     1561 cttgcggtgg cggaagttca gcagaatctg attctgataa agaagtgact ttgacttttt
     1621 ggaatgggtt tactgcatct gatggtgaaa tattgcaaga aatcgttaat gaatttaata
     1681 aaacaaatga taaaaacatc aaaattgaaa tggatatcat gacttgggcc aatttgaatg
     1741 aaaaacttcc tacagcgatt tcttctaaaa aagcacctga ctttattgca ttaaattatc
     1801 cagattttgc acagtatgta gaaaacggag cggtacaacc attagacgat ttctgggaat
     1861 atgatggagt agataaatct gattttaaag aaacggctat tgaattaggt gaagtagaag
     1921 gtaaacagta ttttgtcccc atgcaggtac aaggcatgta tatgtattgg aataatgatt
     1981 tgtttaaaca agctggatta gatactgaac aaccacctac tacttgggaa gaattgacag
     2041 aaatggcacc aaaattaact gatagtagta aaaatgtatc aggttttgtc ttcaataagg
     2101 atggaactgc accgttatat aactggatgt tagcaaatgg cggtaaatta gtgaacgatg
     2161 attatactaa gtctgaattt gcgtctcctg agaaccttaa aacgttaaaa gcgatccaaa
     2221 aaatgatcca tgtagacaag actggtccag aatcgatttc tggcgccgaa atggacaatt
     2281 taatgaatgc tggtcaacta gccattgaaa tcaatgggcc atggttaaat aacggattga
     2341 aagcaaacga gattgattat aatgtgacta ccttaccaca agggagtgat tcagacaaaa
     2401 aaacagctat tttagatggc gtaggatatg ccattccggc aagtacagat aataataaaa
     2461 aagaagctgt ctatgagttt ttgaaatatt ggaatacaac tgaaattggt aaaaaatgga
     2521 gtatcgaaaa tggttttcct gcctacttgg attcagtagc aaaagatgaa gaagtaaaaa
     2581 atgatcctat tgtatctgaa ttatcgaaac aaatggacta tgcagagcca tatttaccag
     2641 gttttaccca aatctctgcg atcaataacg atatcatcaa tccactgatt gaaaaattat
     2701 tagcaggaga agatcctgag acactcatga acgatgcaga tgatgccatc aatcaattac
     2761 tgcaaaaata gtacagaact ttatacagga tagactgttg gcaagataca gatatctgaa
     2821 tgatcgacct agtatttgca gataggtgtt attgcaaaaa tcacagcgat tgttcggata
     2881 tcttattcag attcgtttaa accaagattg tatattttat caaaatgaaa gatgaggtgg
     2941 actatatgaa cggattcacc aaatattgga atcgtcctga tcgtgctgcg tatttgtttt
     3001 taactccatc ttttttagtt ttattattat ttactgtcat accgctttta ggaacatttg
     3061 ctattagttt tacagatttg aatattttct ttacatcaag aaattttgta agttttgaaa
     3121 atttcacacg tatatttagt gatgaacgag cagttaattc gttgattcat tctttatatt
     3181 tcaccgtact cgaaacgcca acacaaatta tcgttggttt attagcagca gtagcattat
     3241 ctaaaaatac cagattcaat cgtttttgtc gttcgacatt ttacattcca gttatttgtt
     3301 cattgacgtc aattgcgatt attttttcta tgttgcttga tccgaatgta ggggcgattc
     3361 cttacttgtt ttcacaagtc ggcttgccga tccctcaatt ttttcgcgat ccaacccttg
     3421 ctatgccgac ggtagccatc atgactgttt ggaagaattt tggtgttact atgacgattc
     3481 tcttgactgc tattcaggga atctctccct ctttgtatga atctgctcaa atggatggtg
     3541 caacaaatcg acagcagttt tttaacatta cgctacctca gatcgttccc tccttaggtt
     3601 tttgtatttt aacgaatctg attggttcga tgcaagtgtt tgaccaagta tatgttgcaa
     3661 ccgctggtgg cccacaattt aagacagaaa cagctgttca atatatttac agtcgaggat
     3721 ttacagcacc ttatgaattg ggctatgcat cagcactgtc ttcggttttg tttatcattg
     3781 ttgcaactat agctatctcc actaatgtgt acatgtcacg gaaagaaaaa caaatgaaat
     3841 aggagggaaa cctgtatggc accagaaagc tcgattacag ttactgtgaa tagtcgaaaa
     3901 gcagttagtg gtaaaaaaat cattcgaacg attagtatgc tagttatatt gttggttatt
     3961 gcgtttcctt ttttgtggct gattatttct tcttttaaac acgaaaaaga cattatctca
     4021 tttccgccta gaatatttgc agatagctat accctagata actatattaa ggtttggaca
     4081 acgatccctt tattagatta catcaaaaat acagtgattt ttgctggagg gacagtgatc
     4141 acttctgtat tctttgattc tcttgcgggg tatgcatttg ctcgtatgcg tttcaaagga
     4201 aaatcagttt tgttctattt cgttttgctg acaatgatga tcccattcca agtcttcatg
     4261 attcctctat ttattgaagt caatttgctt ggcatgcttg atacttatgc aggtttgatc
     4321 attcctcgta tgacaactgc atttggtatc ttcatgatgc gttccttttt tattacttta
     4381 ccagattctt tggaagaggc agcacgcatt gatggattaa gtgagtttaa tatttttttg
     4441 aaaatcatgt tgccattgag caaacctact cttttatcat tagggatttt cacgctgatg
     4501 aatagttgga atgacttgct ttatccattg atattgacaa gtagttcaaa aatgagaact
     4561 ttaccagcag ggttagcttt atttacaggg cagaacattt ctttttatgg acctgtaatg
     4621 gctggaacgg ttatttcaat gctgccatta ctagtggttt atatttttgc acagaaatat
     4681 tttgttcaag gaacagctat gtctggaatg aaagagtgag gaagatgaaa atagttgaga
     4741 ctaaacttta tggacagttg attgtggttt ctgattacat cttattaggt attctctggg
     4801 taatcgtgtg tttgcctgtt attactatcg tgcctgctac atgtgctgtt ttttatgtaa
     4861 tggcgcaatg gaaaggtgag ggatcaggaa atatcattta ttttttcttt caaggaataa
     4921 agcaacattt tggtaagaac ttgttcctta gtctgttaac gatcattatt tattttcttg
     4981 ttaatcaatt attagaagaa cagacaatcg tactttcaat tagtggatat gtagttttac
     5041 ttatttattc catgtttctt ttatcctgga tatatgtgat ttctgaaaac aaagatatca
     5101 catttttaca aatttttgaa caaagtgcat ggaaagtatt ttttgatttt ttgagaaata
     5161 ttctttgttg cgtattgtta ttaggatttg tactgctcat atttcttttc cctcctttta
     5221 tttttatctt tgctggtggc gtatggaaac ttgtatctat tatcttgact ataggaaatg
     5281 aaaggtaggg aaagctgttg gacgaagaag tgaaagtaaa aatcagagag gattttacgg
     5341 taggagagat cagttctcgg ctgtttggct catttgttga gcatatggga agtgtcgtct
     5401 atacagggat ttatgaaccg ggccatgaaa cagctgatgg tgacgggttt agacaagatg
     5461 tcctctctta tgtaaaggaa ttagaattag gtgtcattcg ctatcctgga ggaaacttta
     5521 catctggtta tgattgggtg gatactgtag gtaggaaaga acaacgacca aaaaaaattg
     5581 atttagcatg gcgaggtata gaaccaaatt cttttggact ccacgaattt tttaaatgga
     5641 tcagtcatgt acaagcgaaa ccgatattca cggtgaattt aggaacaaaa ggaattgatg
     5701 aagcaaaaaa tttagtagaa tattgtaatt ttccaaaagg aaccttctgg agtgatttaa
     5761 gaataaaaaa tgggcaaaaa aagccttttg gcgttaaatt atggtgttta gggaatgagc
     5821 tagacggtcc atggcaagtg ggggctaaga gtgcttatga atatggtagg ttagcaaatg
     5881 aagcctcaaa agtcatgaag ttagtggatg attcaattga aacaatctta gttggaagct
     5941 ctacgccacg tttaccatcg taccctgaat gggatcgtat cgcattagaa gaagcttatg
     6001 agaatgtaga ttatttagct ttgcataact atattgataa gtatgatgaa gaagacttga
     6061 ctaaacctcc taaaaatgag cgtgatgata cagcgacata ccttgcaaaa gcttatcgat
     6121 tcgatcgtca aattgaagaa atcgttgcta cttgtgatta tgtaaaagga tatttgagaa
     6181 gtgagaaaac aatgtatcta gcgtttgatg aatggaatgt acatgcccaa ccggaaaaaa
     6241 gacataggga ttttgaagtc ggtagtccca tcgattggtg ttactttact atggaagata
     6301 cattattatt tggttcatta gggctggcta ttttacgaca cgcagatcgc gtaaagatta
     6361 gttgtcaatc attgttagta aacacaatac ctttaatact gacagatgct aaaggacaag
     6421 catggaggaa tccaacttat tatgtgatgc aacacttatc gaaatatgct aaaggtcaag
     6481 tattgaaaca acaaatcatt tgccctgttt atgattgtga aaagataaaa aatgttcctt
     6541 atgtggacag tgtgatcgta gataacggca atcagctgac tattttcttg atcaatcgca
     6601 caaatgaaca acaaaagata acgattgaac atgatttcaa tttgctagat caaggagagc
     6661 actacacatt aaccagtagc gatctgttgg acaaaaatac acgtgaagaa ccagaaaaaa
     6721 taaaacctga aataaactat ttttcaatag aaaaacatga cgaattaaat gtagggatag
     6781 aaaaatactc ttggaatgta attagaataa agaaaatgtg aggaaatttg ttatgatgaa
     6841 gacactttat aataatccta tcgttcggca acgggcagat ccttgggtat ataaacatac
     6901 agatggttat tattatttta caggatcggt tccaggctac caagtgattg aattaagaag
     6961 ggcaaagtca ctgaatgaat tagaacatgc tgcaagcctg attgtatggc aggctcatga
     7021 agaaggaccg atgagtgaat tgatctgggc acctgaagtc cattatatca atggaaaatg
     7081 gtatatttac tttgctgcaa gcaatcataa aactatacgt gatgaatctc atcatcaccg
     7141 catgttcgta cttgaaaatc agcatataga tccaatgaat acagattgga tagaaaaggg
     7201 acaaataaaa acgaaaaggg aaactttcag tttagacgga acaactttct cttctcaagg
     7261 aaagctatat tatgtgtggg cccaacaaga tcctgaaatt actgggaatt ctaatcttta
     7321 tctatccgaa atggataatc cttggacgtt aaaggggaag cagatcctcc tttctatacc
     7381 tgagtatgaa tgggaaaaac ggggtttttc agtaaatgaa ggtccagcag tccttactcg
     7441 caatgggaaa atattcatta cgtattctgg tagtgcaaca gatgaaaact atgccatggg
     7501 cctgctgtgg gctgatgagc acaagaatct tttattaaaa gaatcatggc ataaattatc
     7561 ggagccagta ttcgtttctt ctgaaaaaaa taagcaattt ggtcctggtc acaactcgtt
     7621 tacggtttca gaagacgggt gctttgatat tttgatctat catgctcgac cagagaagaa
     7681 ccaaaaaggg gatccgttag acaatcctaa tcggcacgcg aacgcgcaat gctttacgtg
     7741 gaatgagcaa ggatttccag aatttggtga acctgttccg tatgccttat gagcacaaga
     7801 aaccttgatt tttgcttgaa taaaccttgc agactcgtta tgatatatgt acgtacaaat
     7861 aaaaaggggc aaggcaaatg gcaaaatatc aagatattgc agaagagata aaacaaaaga
     7921 tccttcgtga ggaatacgaa gtaaatacaa ccattccctc agaattcaag ctgcaagaaa
     7981 tatatgacgt cagcagacat acgattcggc aagcaatcgc tgtgttggta aatgagggct
     8041 atctgcgtaa agaaaaaggg tcgggcacat atgtagatga cagctataag aaagaaaaaa
     8101 caccattgaa cagaacaaaa acgatcggtg tcatcaccac atatctctct gattatattt
     8161 ttccgtcgat tatccgagga atcgaaaatg aattacgaga atctgggtat tcattgcttt
     8221 tagccagtac gaataacgat cacgaattgg agaaagagtg tttgaagaaa atgattgctt
     8281 atggggtaga tggtttgatc gttgagccta cgaaaagtaa tcagtataac ccaaacttag
     8341 cactttatgt tgctttgaga gaacaaggca ttccgatggt gatgatcaat gctgcttatg
     8401 aagagttaaa tgcctcttat atttgtttag atgatgtgaa agtcggtttt atggcagcag
     8461 aatatttgat aaaaaaagga catcagcgtc tgttgttcat cacaaaaatc gacgatcttc
     8521 aaggaaaata caggatgaaa ggttttatca gcgcgtgtga acagtataat gtgcgttttt
     8581 tgacggaaga tatcattact ttcaccacgg aaacacgaga aacaatcgct gaaaaaacag
     8641 tacaacggtt gaaagaagaa caagctttag atggcatcat ttgctataac gatcaaatag
     8701 ctagtttaat gctagataag ttgacagaaa atggctatca tattccagat gacctatcga
     8761 tcgtaggaac agacgattct tctttaagcc atttagggaa taaaaaattg acaacattga
     8821 cgcatccaaa agaaaagttg ggacaagatg ccgcacggtg gatcgtcaca acgattgaaa
     8881 aaggacaggc acagccaaat attctctatg aacctgtact agttgaacgg gaaacagtga
     8941 aagaaaacga aaaataagag acgggttttt accgcctctt attgctccta cggttactcg
     9001 tcgcaatgtt tgtgagagct tttgtcatcg ctcaaggctg aacgtttctt tcaccacctc
     9061 tccactaaac ggtgttctcc taccaactgg tcacaagtca caatattcat caaacatgag
     9121 aagctgtttt ttgaaattgt tccttgtgac tggaaactaa gcggttcctt cttatttctt
     9181 gaagctgacc acttctgtca caacctcttt aagaatgatg gatagaaata cgtttaggat
     9241 aagttttttt agatgcactt tgttcgattt cctctaaaga tttcccacga gtttctggta
     9301 cgatctcttg aataaataaa acccctaaaa agcagatgat cccaaaaatg ccgaaaacga
     9361 tttcttgaga catagaagca gtcatcacag gaaaaagcaa gccaactagg aaagaaccaa
     9421 tccaattaaa agaagaggct aggccagatg ctcgcccgcg gacagctagt gggaatattt
     9481 cgcctacgat tacccatgtc agtggagccc atgtgcaaga ataaaatgcg acataaatac
     9541 ataaaaacaa gagaatcaac aacgaatttg tatgagcatc cagtacagtt cctaaaacag
     9601 ctggtaggat gaaagaaagt cccatcacgc taccgcctat tttcaataaa gttcgccgat
     9661 tgaacttgtc tgcaataacc aaaaagagca aagaaccagc aactaaaatg attccttgga
     9721 tgatcggcca catcaatgca tcgctcgctg catgcccagt cgctttttct acgatcagcg
     9781 gaatatagta aaaaatagca ttagctcctt gaaattgctg gaatactgct acaccaatac
     9841 cggcaatgac taaggaacga tatttttcta aaaacaaagt atgccaagaa gttccggttg
     9901 tcttttcttg tttgacattc agctggattt gttcgaattc ctgttcagct tcttttggtg
     9961 tccgaatata gttcaatact ctttttgcct cttccaagcg gccagattgc ataagaaaac
    10021 gcggtgactc tggtagagct aaaacaccga aaaataaaat cagcgcgggg acagcagcga
    10081 gccctaacat cacgcgccaa gccatcgttt caggcaaccc ttttagcaaa taatctgcaa
    10141 cataggaaag gagcatgcca gaagcaatca tcacttgatt gatccctgac aatcttcccc
    10201 ttagtctagc aggagccatt tcagacatat aagcaggaac tagggcagat gcagcgccaa
    10261 cagctaaacc tagaagaatc cttgctgcaa tcagaaaata agaaccattg tgaggggcag
    10321 atgcagaaag gacagaacca gccataaaaa taagtgcaga aagaagaatc atttttcgtc
    10381 gtccaagctt atcagaaaga agaccagata aagagccgcc aaggatcgca cctaacatga
    10441 ccgaagacgt gatccatcct atcaaagaag ctttacctgc caaaccccaa tcatgctgta
    10501 aaaaaggtaa ggcgccggtc ataacaccaa tatcatatcc aaatagaatg ccgccaaatg
    10561 cgccaaaaaa ataaataaac gatgaggaaa cttttttttc ttttttcata agtattctct
    10621 ccttctagaa tttcgagaag gcggttgatc agattttgta agcaatttca ttttataact
    10681 tatgtacgta caaaacaagt gttttttatt gtattcttgt aaataactgc ttgacatgta
    10741 cgtacaaaag tatacaataa tcattgtaag cgctactaaa aagacggttt gatcaggttt
    10801 gacgagaagg agggagttaa atcattatga aggaagtaaa tgcgcaaagt atgttagatg
    10861 tcatagatgg tcgtacatct ttagggattg agtttggatc aactaggatc aaagctgtat
    10921 tgattgattc atcttttact cctattgccg ctggtagtta tgagtgggaa aacaaactag
    10981 aaaatggtat ctggacctat tcattagaag atatttggaa aggactgcgc gtcagttacc
    11041 gaaaaatggc agcagaagta ttcgaaatct atggcgagaa tcttacgaca atcgggtcga
    11101 ttgggttcag tgcaatgatg cacggttatc tagcatttga tagcaaggaa cagttactag
    11161 taccatttcg tacttggcga aatacaatca ctgaagaagc agaacgaata ttaacggaaa
    11221 aatttcaata caatattccg cagagatgga gtatcgctca tttgtatcaa tctattttga
    11281 ataaagaaga acatgtgaaa gaaattaatt ttttgacaac gcttgcaggt tatgtccatt
    11341 ggcagcttac tggtgagaaa gtactaggcg taggagatgc atcaggcatg tttcctatcg
    11401 atccttcaac gaaaacctac catcaagaaa tgaaaaatat ttttggtcag ttaatcgaaa
    11461 aagcgcacgt agcgattgac ttggatcaca ttttgcctaa agttctagta gcaggagaag
    11521 aagctggcaa gttaagcgaa tcaggggctt atctgatcga ccctacggga aatttgagac
    11581 caggaatccc ggtttgtccg ccagaagggg atgcaggaac cggtatggtc gctacgaaca
    11641 gtgtagccaa agggacaggg aacatttcgg ctggtacttc cgccttcgct atgatcgtat
    11701 tagaaaaaga actagaaaat gtccatccgg aaatcgacat ggtcactaca ccttcaggag
    11761 aactagtagg catggttcat acgaataact gctcttcgga tatcaatgct tgggtcagta
    11821 tttttgaaga atttaccaat tctttgggta tggagataag cagagataaa ttattcacgg
    11881 tattattcaa caaagcgcta gaaggcgata cggattgcgg gggattactt tcttatggtt
    11941 atttttccgg tgaaaacatt acaggagtaa atgaaggaag gccattattt gtacgtacac
    12001 caggtagccg ttttgattta gcgaatttca tgcggatcca tttagccagt gcttttggtg
    12061 caatgagaat cggaatggat atattgaaat ctgagaatgt ccaaattgat cgtttagtgg
    12121 ggcatggggg gatttttaaa acaccagaag ttggacagcg gattctagca tctgctatgg
    12181 aggcaccagt tacagtgatg gacacagccg gagaaggtgg agcgtgggga atcgcgttat
    12241 tagcagccta tatgatggat cgacatggga agtctttaga aaacttcttg gcagaagacg
    12301 tgtttggaca agaccaagga atcaccatca caccttcagc agaagaaata gcaggctatc
    12361 agatatttat gagacgttac cgtgagggga tcgacattga aaaaacagcg attcatcaat
    12421 tgcaagaaaa aggagtgata gaaaatgctg gaacaactga aagaagaagt ataccaagca
    12481 aatcttgact tgcctaaaca cggactcgtc aaatatacat ggggaaatgt gagcgctttt
    12541 gatcctgaaa cacgatattt cgttatcaaa cctagcggag tcagttatga agagatgaca
    12601 gcagacgaca tggtcgttgt ggacttggac aatcatatca ttgaaggtaa actcaatccc
    12661 tcttcggata cgcctactca tgctgtttta tatcgttcat ttcctcaaat cggaggaatc
    12721 gtccatactc actcgacatg ggcgacgatc tgggcacaag caggattgga tgttcctgcg
    12781 atggggacga cgcatgcaga tactttttat ggatcggtac cttgtgctcg atttttgaca
    12841 caacaggaaa tcgacagcgg gtacgaatat gaaacaggaa aagttatcat agaaacattc
    12901 aaagaacgca aaattgatcc cttagcaatt ccaggggtac ttttacacgg acacggtcca
    12961 tttacttggg ggaaagatgc gcaaagtgcg gtaatgaacg cagtcgtact agatgaagta
    13021 tgcaagatga atttatttac cagacagctc aattcatttt cagaagagct gcctcaacgg
    13081 attttagata aacattattt acgaaaacac ggaaaaaatg cgtattacgg acaaaacaaa
    13141 taaaaggagt ggctaattat gttgaatatt ggagaaaaag aattttggtt tgttgtagga
    13201 tcacagcatt tatatgggga agaagcactg cgagaagtaa agaaacatgc acaagaaatg
    13261 gtcgatgaac tgaatgaaaa tggccaattg ccttatccaa tccgccttca agaattagca
    13321 gttactgctg atacgatcac aaaaatcatg aaagaggtca attatcgaga agaagtagca
    13381 ggtgtgatca cttggatgca tactttctca ccagcaaaaa tgtggatccg cgggacaaag
    13441 cttctacaaa aaccattgct tcatttagct acgcaatata acgaaagcat tccttggaaa
    13501 acgatcgata tggatttcat gaatttaaat caatccgctc atggtgaccg cgaatatggg
    13561 ttcatcaatg ctcgattaaa taaacaaaat aaaatcgtcg ttggctactg gaaacgacct
    13621 gagatccaaa aagagattgc tgattggatg gatgttgcag tagcctataa tgaaagtttt
    13681 ggaataaaag tggctcgttt tggtgacaac atgagaaacg tgggtgtcac agaaggagac
    13741 aaagtggaag cacagattca atttggttgg actgttgatt attttggtat cggagatctt
    13801 gtacaagtaa tcgatcgcgt atcagacgaa gaagtcgatc aattatttga agagtacaag
    13861 gaattgtata ccttcgacta tggtgattat gaagaaaaga catgggagga acatgtaaaa
    13921 gtacaagctc agcaggaaat cggtattcgc cgtttcttag aagaaggagg ctataacgct
    13981 tttacgacta attttgaaga cctgtatgga atgaaacaac tacctggact agctgtacaa
    14041 cgtttgatgg cagaaggata cggttttgct ggggaaggcg actggaaaac tgcagcgatt
    14101 gatcggttgc tgaaaatcat ggctcgaggg aaagatacag gattcatgga agattatact
    14161 tacgagctag ctagtggcca agaagcaatc ttggaatccc atatgatgga agtagatccg
    14221 acgcttgctg cgacgaaacc aagaatcgtt gtttctcctt tatcaatggg agatagagaa
    14281 gatccggcgc gtcttgtatt tgatgggaaa gcaggagaag gggtagtcgt atcgatggca
    14341 gatttcggta cgcattataa actgctgatc aatgaagtag aagcctttga accaacgact
    14401 gaagcaccta atttacctgt tgcacgtgtg ttatggaaaa ctaaaccgaa tttccatgaa
    14461 ggtgtccatt catggattca ggcaggtggc ggtcatcata ccgttgtctc gctaaatcta
    14521 acaacagatc aaattgaaac gtgggcaaaa ttagttgagt tggaaacagt ggttattaga
    14581 taaaaagagg ttgtgaaaaa gccacttagt tttaagaaat aagaagagat tgacgaaatt
    14641 tgcttttcaa cttttttgaa atttagtctt attatcaatg tcagctaacg gaacaccatt
    14701 tatcaagata tctcaccctc aatattttgc tctcgaaaat aaggcttatg gcagtaactt
    14761 gtcatagcct tatttttatc actcagccta ctctgctcta actgctcttt ttgcttctag
    14821 gaagtcaaat ggtaaacagt tgtctaattg cagaaatttc gagatacttg ttagtaagga
    14881 gggggtttta tgtatcacgt tcatttgatt gatgctattc cagaacaaga gatagaaaag
    14941 ttacaagggg aatatttcaa aataatagat gatttgccag ccgaaggaat agttattcgt
    15001 agtacgaaag tgaaagatga atggcttaca ccagatttgc tagcaatttc aagagctggt
    15061 gttggggtga acacgatcaa cgttgaaaaa gcttctgaaa acgggacgat cgtcatgaat
    15121 acaccaggtg taaatgctaa tgcagtaaaa gagcttgttt tgtgctgttt gctcttatct
    15181 tcccgtccga taatcgaagc aagtcgtatg gtacaaacac taacaggtcc taatatttta
    15241 gaacaggcag aaaacaaaag aagtgcttat gtgggacgag aattgcaagg caaaaccatc
    15301 ggtcttcttg gtcttggagc gataggcaca aaagtagcgc taagttgcta tagcttaggg
    15361 atggatgtct tgggttattc tatccgcgat gcccagttgg attatgttcg gcaagcagat
    15421 ttagaaacag ttctttctac ctcggattac atcgtagtca tgctgccgtt gacagaagat
    15481 acaaaaggat tgatcgatca ggcgaatata gaaaaaatga aaaaagatgc tgtacttatc
    15541 aatgtaggaa gaagtgaaat tgttgataaa tatgctgtta tgcaagcatt ggaaaaacaa
    15601 catctggcta aatacttgac tgattttcca gaagaagagt tcttagaaaa tgaccgtata
    15661 cttatgttgc ctcatctagg ggatcgactc aggaagcttt ggcagacagc ggacgtttgg
    15721 cagttgaagc cttaaaagat tatcttttgt tcggtacggt tagagaagcg gtcaattatc
    15781 catccgcacg aatgtttttc caagcgccgt ttcgattcac tatattttat caaaagaaaa
    15841 ccaatatatt agcagagata ttttctctgc tgaatgagaa cgagctagcg atcgcagata
    15901 tatcgcgtaa ccataagaat gagcatgtct acatattgat tgacatagac agtactgatt
    15961 ttgaacagct caatcaagtg aaacaacagc tagaaaaaat ttctggagta cgaaaggtcc
    16021 gcttattaaa gaaaccagaa aggccataaa gaaagagaat atttggattt cattaattct
    16081 tctttccgtt ctttactttc gaatttcgta caactaaaat aagagaagca acattctttt
    16141 attgatagaa atgaaggagc aagcatttga aaagatatat aagcagactt tcgaccaaac
    16201 agaaaaagat tttaaaagga acagcagcaa tcatcagtat cttttttctt gttgttttta
    16261 gtaatttatt tttgcagtgg tgtcaaaatg atctatccgt agaccttgcg ttgaaatttg
    16321 ctttttcttg gcatacggaa aaattcttct tagcctgtct cgtcttgctg attattctta
    16381 tttttctgat tgcactagca ggatctgtcc ctttaggttc ccttacgtat gtcgtagcca
    16441 tcggtgtatt aggttttgca aattatatga aaatgagtta tcggcaagaa ccgatttatc
    16501 cagacgattt gaagatgatc actgaaatcg gattgctgaa agatatgacc ggcactatgc
    16561 tgtttacagt gatcttagct gccgcaggaa ccgtgatagg attgttttgc tggtacatgt
    16621 ttcgaagcct gaaaaaagga aggcgattcc aattgatccg attaacgaca ctgcttgtgg
    16681 caattggact gctagggtat atcagtaatt tcaacaatcc cgataatttg ttgcgaaaag
    16741 catataataa aacggcacta tggatacctt acagccagaa aatgaactac tataacactg
    16801 gttttatcgg tgggtttttg tacaatctga aggtagaacc aatggatgaa cctgaaggat
    16861 attcgaaaga aaagatcaaa gaaatcactg aaaaatacca gaagctagcc gatgaaaaaa
    16921 ataaaactgt agaggaagag tcgccgaaca tcgtgttcgt gatgagtgaa agtttctcag
    16981 atcctagtcg attaaacggc gtagaagtat ctggtgagcc attggcagat tattatgaag
    17041 tagctgatca aacatatagt ggcaatatgc tttctcaaaa ctatggcggt ggaacagcaa
    17101 atatcgaatt tgaagcattg actggttttt ctatggcgtt atttaatgcg caactcacca
    17161 ccccttacac gatgcttgta ccaaaaatgg atcagcttcc ttctatcgtt tcagcgttaa
    17221 acgcacaatc ataccagacg acagcgatcc atccatacaa tacttcgatg tataaaagag
    17281 aggatgttta tcagacatta ggatttgacc agttcatcag cgagcgtacg atgacctata
    17341 cggatacgat agagaataat ccatatattt ctgatgagtc agcttacaaa gaagtaatga
    17401 cacttctcaa ggaagaaaaa acacctcagt tcattcattt agtcacgatg cagacacata
    17461 tgccctacga tggaaagtat gataatctag actatcctgc aaaaacttca gatggaagtg
    17521 ggacacttga cttagagaat tatctgcaag atatttccta tagcagcacg gcactgaagc
    17581 agttcacgga agaattgaag aatctttcta gaaggacttt agttgttttt tggggggatc
    17641 atcttccagg gatctattca gatacgatcc aagcaaaaaa tgacaaacaa acattgcatg
    17701 aaacacaatt tcttatgttc gacagtaaag gagaattaga aaagcaaacg actcacgatg
    17761 caatcaccag tccgttttat tttgcagcga atctaatgga acagacgaat cagacaacaa
    17821 atggctttta tcagctgctt ttatcattag aacaggaact tccggcgttt gaacgagaac
    17881 tgtactatca aaacggacag tggtataaag aagcgcaatt caatcgctcg caacaagaga
    17941 tttacgacga atatcaattg atccaatatg acattgtcgc agggaaacaa tatagtttag
    18001 cagatggttt ctttgagcac gaataagaat ggcgagctgt atagaaacca ttaaattttt
    18061 tcaagtactt gtcatttgag gataaaaaca agtagaataa ataataacaa caatggagaa
    18121 agtaggaaaa cgaacatgtt accgaatatt ggctattatg tgcagtccat cgatcaaatc
    18181 gtcaaggaaa cagaagcaat cggagaagga atgcatccga aatatgaagt gatcagaaac
    18241 gcaattgatg aaggaaaaac aagtgaactg gacaaatcga ctcttgaaga gatcatcctg
    18301 ttatttgaag aaggaacaaa aaaatatcgg gcaatgctta tgaaagtcag tactttgcgt
    18361 ccaccagcaa aagcaatggg cattcataaa aattttgaaa aagcttatct ttcatatgta
    18421 gcaggctgtg aagaaatgat ccaaagtatc gatcttgaat caggggtaga tgaagatcta
    18481 ttcaatacat ctgaagcaaa acaagaccaa gcgacagacc aattgacagc tgccatccaa
    18541 aaaatgacta atttgttgtt gaaaaaataa aagaaatctt tgataacaat aaaaactaga
    18601 gttatctaat ataaatgtaa tattaccttt atgatagagt gatcaaaatc ctttatgata
    18661 atgacatacc tataaccaac aacaaccttg tacttactca aaatcgagta aatactcctt
    18721 ttccgtcgag tctaaccatc tcggcggttt tttatttgta gaaaagaaca tcaggataga
    18781 cggaaaagtc ttgtcctgat gttcttttca gatagtatgg aaagctgatt agttttctac
    18841 attctgtaca tattgttcaa agtagttctg taaatcggct ttgagtttct gatattcctc
    18901 ttcgctatac tctttatctt cgattttccc atcaaagaaa ggaatattta gttcttcccg
    18961 tagtctgtct aatacgtctt gctgattcat ttcattctcc ttttcttact aacgatcaaa
    19021 ataaacacag acacattctg gtacatagac atcttttttc agtaattcca gtaatcctgt
    19081 ttcaggatga cggcgatata atgtcagatt atcggtgttc tgattggcac acacgacaaa
    19141 attattgctt ggatctagtg cgaaatcgcg gggaaaatcc ccttctgttg agatgatttg
    19201 cttgttttga atgtgggaac catcttcaaa aactgcgaat acagcaatcg agttatgtcc
    19261 tcggttagaa gcataaagga acttgccatc atttgaaatg cggatagctg cccctccatt
    19321 ttcaccgtcg aattcttctg gcaaggtcga tattttctgt aattcttcaa aagcacctgt
    19381 ttctttatta taagaaagca cgcggactgt gctgtctaat tcaccaaata aataagcgaa
    19441 ttgtccattt ggatgaaaga caagatggcg agggcctgtt cctggctctg cagtgaaggt
    19501 agttacaaga ttcaatttgc cgttttcgga cacatcataa gtatacacac gatctgtacc
    19561 aagatcacat acgactaatc gttggtcagg agtcagatcc gtatagtgga catgcgcatg
    19621 atcttggttt ttatgaggac ctgtcgcttc ttgatggaat a
//
LOCUS       DACEXQ010000025        19402 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.025, whole genome shotgun sequence.
ACCESSION   DACEXQ010000025 DACEXQ010000000
VERSION     DACEXQ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.025"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..359)
                     /locus_tag="IXH04_000729"
     CDS             complement(<1..359)
                     /locus_tag="IXH04_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1851360.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQIL"
     gene            616..4005
                     /locus_tag="IXH04_000730"
     CDS             616..4005
                     /locus_tag="IXH04_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAQ1851361.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            4008..5429
                     /locus_tag="IXH04_000731"
     CDS             4008..5429
                     /locus_tag="IXH04_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ1851362.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            5426..7303
                     /locus_tag="IXH04_000732"
     CDS             5426..7303
                     /locus_tag="IXH04_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ1851363.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            7395..8237
                     /locus_tag="IXH04_000733"
     CDS             7395..8237
                     /locus_tag="IXH04_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1851364.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(8389..9264)
                     /locus_tag="IXH04_000734"
     CDS             complement(8389..9264)
                     /locus_tag="IXH04_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1851365.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            9471..10502
                     /locus_tag="IXH04_000735"
     CDS             9471..10502
                     /locus_tag="IXH04_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAQ1851366.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            10591..10896
                     /locus_tag="IXH04_000736"
     CDS             10591..10896
                     /locus_tag="IXH04_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAQ1851367.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            11075..13090
                     /locus_tag="IXH04_000737"
     CDS             11075..13090
                     /locus_tag="IXH04_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ1851368.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            13092..13376
                     /locus_tag="IXH04_000738"
     CDS             13092..13376
                     /locus_tag="IXH04_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1851369.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            13402..14775
                     /locus_tag="IXH04_000739"
     CDS             13402..14775
                     /locus_tag="IXH04_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ1851370.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(14858..16873)
                     /locus_tag="IXH04_000740"
     CDS             complement(14858..16873)
                     /locus_tag="IXH04_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAQ1851371.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(16895..18109)
                     /locus_tag="IXH04_000741"
     CDS             complement(16895..18109)
                     /locus_tag="IXH04_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1851372.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            18330..19274
                     /locus_tag="IXH04_000742"
     CDS             18330..19274
                     /locus_tag="IXH04_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1851373.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
BASE COUNT         6588 a         3616 c         3662 g         5536 t
ORIGIN      
        1 tctaaaattt gattgcattg tttcaactta cgtgtaaaag aagaccggct gatggaaatt
       61 ttttcacaat aatactcaat cgttttaccc ggattgacaa taatctcatt taataaacga
      121 ctgtttgtag aactttgcga catttccatc agaacattta aataagctgg tacattcaaa
      181 tgttcaagac gataccccaa tttttgtgaa gattgaatcg ttacatccgt tccccagcgg
      241 attttgatct cttcgatata ccttacaaat gtagattctg aactaccaat tcgtttggca
      301 agttcttttt tagtcatcca cttgtcagaa atggacagaa ttcgaatgat tttttccatc
      361 tttctatagt ttcgttccaa caacctgatc atatgttttt ctccctcaac ccttttcttt
      421 caatgaaacg ctcattaatc gaaggaatca acttcataaa tgaaaccaat tggtatgaaa
      481 attggtgtga ttccctttag cgcccttcgt ataataatta tcatattaaa ataaataaaa
      541 ttatgtaacg cctgtttatt ttaataattt tcactattca cgtcaaaaca gaaaaaaaga
      601 aaaaggaggg gaaaaatgac gaccacaggg aagaaactga aagttatttt catgctgata
      661 atattgagtt tatcaaactt tgtgccatta tctgcaatag cagacactac agatgatcca
      721 acagttttag aaacaatttc agctgaagtc atttcggatc agtctggaaa aaaagcactg
      781 aacatcaagc taaatgcgaa taacaccagt gctgaaaaga tagaaaaaga aattggtcta
      841 gtcgaaaatt acttaagtga tgtggaaaga aaagaaggag atggctatgc ttatcaggta
      901 aatagcggga aaattacgtt ggaaatctca tcaaacacta aacaaactat cgatctgagt
      961 tttccaatcg atccagcact ttaccacagc caggcaaaca agctgatcgt cgataataaa
     1021 gaatatgaca ttattgatga gacagaaaat aagaaagata cagatgtgtc agtaccaaag
     1081 ccagacgaaa tagaagaaga atcatcaaaa gaaaacgaaa attctgtcag cccatttaca
     1141 ttgcctacat tatccttgcc agctgtgagt gtgccatcta atcaaacgat tcctacagaa
     1201 tatacaacag atgatcaggg cacttatcct aaagccagtt ggcaacctac aggaaataca
     1261 aatgttcttg atcatcaagg caataaaaac ggaacaaatc aatgggatgg tataaattct
     1321 tggaatggag atcctaatga tcggacccat tcgtatatcg aatatggagg aaccggtaat
     1381 caagcagact atgcgatacg aaagtatgca aaggaaacaa gtacacccgg attgtttgat
     1441 gtttatttga atgctcgtgg aaatgtacaa aaagatatca cgcctcttga tctcgtattg
     1501 gtcgtagact ggtcaggaag tatgaacgac aataatcgga tcggtgaagt aaagattggt
     1561 gtcgatcgtt ttgtcgatac tttagcagat agcggtatca cagacaaaat caatatggga
     1621 tatgtcggct actcaagcga aggatatagc tacagtaacg gtgcagtaca gatgggttca
     1681 tttgattcag tgaaaaatca agtaaaatcc attacacctt cacggacaaa tggtggtact
     1741 tttacacaaa aagcactaag agatgcagga agcatgctat ccgttccaaa tggacataaa
     1801 aaagtgatcg ttttgctgac ggatggtgta ccaacatttt cctataaagt acagcgggta
     1861 cacgcacaat caagcagcaa ttattacgga actcagtttt ctaatacgca agatcggccg
     1921 ggaaatactt ctctaatctc aagaatctat gatgcacctg accaaaacaa tctatccaga
     1981 agaatcgaca gtacgtttat cgcaaccatc ggagaagcga tggcactcaa agaacgagga
     2041 atcgaaatac atggtcttgg catccaactt caaagcgatc cggcagctgg tctctcaaaa
     2101 gcagaagtag agtctcgtat gcgacaaatg gtttcatcag atgaaaaagg cgatctttac
     2161 tatgaatcag ctgatcatgc aacagatatc tctgaatacc tagccaaaaa agctgtacag
     2221 atctcagcaa ctgtaagcaa tggacaaata aatgatccaa tcgcagaacc attcatttat
     2281 cagcctggta cactttcagt caagagtgtg gggacaagtc ctacaacggt cactccatct
     2341 atttccatag aaggaaatac catcaagagc aatcagatct atttaggaaa agaccaagaa
     2401 atccaaatcc attaccaagt gagaatccaa acagaaaatg aggacttcca tccaaatttc
     2461 tggtatcaaa tgaacggcag gacaactttc cagccaaaca ttgataccaa tgaattagct
     2521 gaattcggta taccatctgc taaagctccc ggagtcagtc ttcacatcaa aaagttatgg
     2581 gaagaatttg acaacaatct agctgatcgt ccagatcaag ttacttttga gattcaacgg
     2641 gaacatacga caaatgctgc agcttggaaa aacggatata ttcgaatcat taaaccagct
     2701 aaagatacaa caaatacgtg ggaacgtgca gacattgaca aattatctgc aaatagcgga
     2761 gaaagttatc aagagatatt atcactacct caatacaata atcaaggtca agcattcagt
     2821 taccaaacaa tcaaagaatt acctgtacca ggatacgatt ctcaacaaat agatgcaatg
     2881 acatggaaaa atactaaaca attcacaccg ttaaacttga aaataacgaa aaattcctct
     2941 acaggtgaaa aggatcttat tggcgctgtt ttcaaattaa caggagattc tattgatact
     3001 ttactaacag atcatggcga cggaacctat tctcttccag aaaatgtcaa attgcaaaaa
     3061 gaaatgacct atacgctgac agaaacaaaa gctccagaag ggcatggatt aagcaaaaag
     3121 actacttggg aaatcaagat cgcttctgat ggtacggtaa ccattgatgg aaaaacagtc
     3181 actacttccg atgatacgat ccagttgact attgaaaatc cttttgttga agttcctgta
     3241 gcagtacgta agtatgcgat gcaagggacg gacaaagaga taaatcttaa aggagcagca
     3301 ttttccctac agaaaaaaga agcaaatggt acttatcagc caattgacag ccaaacaacg
     3361 aatgaaaaag gtcttgccag ttttgattca ctcacacctg gtaaatatcg agtcgttgaa
     3421 acagctggtc ctgccggata tgatacttcg ccgggaaatt atgaattcca aatcgataaa
     3481 tatggaaaaa tcatttacac gggaaaaaat accgagatga caaataatgt atggacgctc
     3541 actcatcaaa atcgactaaa agcgtttgat ctaacggtac acaaaaaaga agacaacgga
     3601 cagacattaa aaggagcaaa attcagactg cagggaccag aaatggactt agaatcgcca
     3661 aaagatggac aagaaacaga tacctttcta ttcgaaaatt taaaacctgg aacttatacg
     3721 ctgaccgaaa cttttacacc agaaggatac caaggtctaa aagagccagt tactatagtt
     3781 atacacgaag atgggtcaat tcaagtggat ggacaagatc atgaatctgt tctgtcacca
     3841 ggagccaaaa acaaccagat ttctttagac atcacgaatc aggcaaaagt accattacct
     3901 gaaacgggag gaattggccg tttaggaatc tatctagtag ggatgattgg ttgtgcgttt
     3961 tctatttggt atcttttttt gaaaaaagaa agagggggca gctaaatatg aaaaaacttg
     4021 gttggcttag tatgtgtctc ttcttgttac tatttaaacc agcttttact caggtagcaa
     4081 cagaaacaga aacagaaatg gttcagatta ctttacacaa attgcttttc ccaaacgggc
     4141 aactgccgaa aaatcatcca aatgacggac aagaaaaagc tttattacaa acgtatcgag
     4201 gattaaatgg tgtcacattc caagtttatg atgtcacaga ttctttttac catctacggg
     4261 aaaagggcaa aacggtagaa gaagcacaag cagagatcgc aaaaaacggt gcgtcttccg
     4321 gtatgtttac cgcagaagca acaactacaa ctcttaacaa cgaagatggt atcgcttctt
     4381 tttctctggc cgctaaagat caagaaaaaa gagataaagc gtatcttttc attgaatcca
     4441 aagtaccaga agtcgtcaaa gaaaaggcag agaatatggt agttgttctt cctgtacatg
     4501 gacaaaacaa tcaaaaactt tcaactatcc atttgtatcc taaaaatgaa gaaaacgact
     4561 accctgatcc accttttgag aaggtattag aagagcctag aaatgatttt acgattggtg
     4621 aaaaaatcac ttattccttg catacgacaa ttcctgtaaa tatccttgac tatcaaaagt
     4681 tcgaattgtc agatagtgcg gatgaagcat taacgttttt acctaatagt ttaacgattt
     4741 catcgaatgg agaaaagctg acagaaggct ttgtcataca caagaaacct cacggatttg
     4801 atgttttatt ttcgatccct tcgttggaaa aatatgctgg aaaaaaactg accatttctt
     4861 atcagatgca gctaagcagt acagcacagg cgaacaagga aatcaacaac aacggaacac
     4921 tggattttgg ttttggtgtc agtacaaaga aagtctctgt atatacaggg agtaagcaat
     4981 ttgtcaaaat cgagacaaat aaaccagata aacgattagc tggcgcagta ttccttatta
     5041 aaaacaaagc aggaaattac ctccagcaaa cagccaacgg atacaagtgg acaaagaacg
     5101 aatcagatgc gcttcacctg atttccgata aaaatggcgc tttttcaatt tccgggttga
     5161 aaacaggaag ttatcgatta aaagagatcg aagcaccttc tggttatatt ttaagtgaaa
     5221 cagaaattcc gtttaccatt tcaacttttc tttctgagga taaagaggcg gacagtatat
     5281 tgaaagtagt caataaaaaa gaaaatagcc gtccatttct tccaaaaaca aacgaaacga
     5341 aaaatacact tttaggcgtt gttggtatgg tattcgcaag ctttgcaatc tggttgttta
     5401 tcaaaaaaag aacaggagtg aaaaaatgaa aaatcataaa aaaataaacg ttatgttagg
     5461 agtccttttc cttattttac cattactcac aaacagcttc ggcgcaaaaa aagtgtttgc
     5521 agaggagaca gcagctcaag tcatccttca taaaaagaaa atgactgatt tacccgatcc
     5581 tttaatccaa aacagcggga aagaaatgag cgaattcgat caataccaag gattagccga
     5641 tatttcattt tcagtttata acgtcactca agaattttat gcgcaacgag ataaaggagc
     5701 gtccgtggat gcagcaaaac aagcagtcca gtctttgact cctggtacac cagttgcttc
     5761 aggaacgaca gatgctgatg gaaatgtcac tttatcttta cctaaaaaac aaaatgggaa
     5821 agatgcagtc tacacgatca aagaagaacc aaaagacgga gtgtcagctg ccgcaaacat
     5881 ggttttagct ttccctgtat atgagatgat caaacaagca gatggctctt ataaatacgg
     5941 gacagaagaa ctagatacta tccatctcta ccctaaaaat acagtcggta atgatggaac
     6001 gttgaaagtt acaaaaatcg gtactgccga aaacgaagca ctaaatggag cagaatttat
     6061 tatttctaaa gaagaaggaa caccaagcgt caaaaaatac atccaaagtg tcacagatgg
     6121 attgtacact tggacaactg atcaaaccaa agccaaacat ttcattactg gtcattctta
     6181 tgacatcggc aacaatgact ttgccgaggc atctattgaa aaaggccagt tgatcgttaa
     6241 tcatttagaa gttggaaaat ataatttaga agaagtaaaa gctcctgata atgcggaaat
     6301 gattgaaaag caaacaatca cgccttttga gatcctggca aatagccaaa caccagtaga
     6361 aaagaccatc aaaaatgata cgtctaaagt tgataaaaca acacctcaat tgaatggaaa
     6421 agatgtcgca atcggtgaaa aaattcaata tgagatttct gtcaatatcc cattaggtat
     6481 cgctgataaa gaaggaacgc aaaacaagta cacaacattc aaacttatcg atactcatga
     6541 cgctgcttta acatttgata atgattcttc aggaacgtat gcttatgcct tatatgatgg
     6601 aaataaagaa atcgacccag taaattattc tgtcactgag caaacagacg gattcacggt
     6661 ttcagttgat ccgaattata ttccttcatt aactcctggc ggtacattga aattcgttta
     6721 ctatatgcat ttgaacgaaa aagcagatcc aaccaaagga ttttctaacc aagcaaatgt
     6781 cgataacggg catacaaatg atcaaacacc accgtcagtc gatgtcgtta ctgggggcaa
     6841 acgatttgtt aaagtagatg gtgacgttac atcagaccaa acacttgctg gagcagaatt
     6901 cgtcgttcgt gatcaagata gtgacacagc gaaatattta tcgatcgacc catccacaaa
     6961 agccgtcagc tgggtatcgg cgaaagaatc agcaacggtt tttacaacca caagtaacgg
     7021 tttaatcgat gtgacaggtc taaaatatgg cacgtactat ctggaagaaa cgaaagcgcc
     7081 agaaaaatat gttccattaa caaaccgtgt agcatttact atcgatgaac aatcttatgt
     7141 aacagcagga cagttgattt ctcctgaaaa aataccaaat aaacacaaag gtacacttcc
     7201 ttcaacaggc ggtaagggaa tctatgtgta tatcggtgca ggagtagtcc ttctactgat
     7261 tgctggactg tactttgcta gacgcaagca cagtcagatt tagtcttttt gcaagaattg
     7321 gaaaaaggag cagcggatta cgcaaatgct cctttttctt ttattatcaa acagtcatca
     7381 caaggagaac acaaatgaaa ccaaagaaaa ggaaacttct agatctgctg atgcttttct
     7441 tgcttctctc aggaataggg atactagctt atccatttgt tagcgacgcg ctgaataatt
     7501 atctggatca gcagatcatc tctcattatc agcaacaagc ggtcaaagaa aatgaagaag
     7561 tcatggctaa aatccaagaa aacatgacaa agaaaaacca gcaattagca aaagaaggtg
     7621 gaaatccagg tgcagatccc tttactaaga aaaaagaacc tgcgaaaaag gatagaacct
     7681 attttgagaa acatacgatc ggtattttga cgatccctga gatcaatgtc catttgccaa
     7741 tttttgatga aacgaatact gtgctgctag aaaaaggtgc ctcattattg gaaggaacgt
     7801 cttatcctac ggggggagaa aatacacatg ccgttatttc cagccatcga ggactgcctc
     7861 aagcaaagtt gttcacagac ttaccgaaac tgactttagg tgattctttc tatatcgaaa
     7921 tcaatggacg gacactcgct tatcaagtcg atcagatcaa gacggtagaa ccgactgata
     7981 caaaagatct gagaatcgaa aaagggcagg atttcgtgac actcctaact tgtacacctt
     8041 atatggtcaa cagtcatcgc ttacttgtca gagggcaccg ggttccttat catgcaaaag
     8101 aagttcaaaa agaagtacag aaagtatccg agaacaaacg atttttcctg tatggattgt
     8161 tagctgcatg tatactgatt ttcctccttc ttatatatct ttttcgtcaa tcgttcaaaa
     8221 taagaaacag aagttagcta ttcctcattt ctataatata tctattcgaa tcgttccgta
     8281 aacaaacagg ctgaggtttg aaaaagaaaa cttcagcctg tttttatgat gatatcaaag
     8341 agagcctatc aaacactatc tttcttatta ctaaatgagg atcttttctt aagttttccg
     8401 atattcttta agaaggcctt tttgcatcaa ttcttgtttg tttttcaagt tgcggatatt
     8461 ctcttcaaaa ttttgagaat atatcactga gaataacgta tttaatacgt aatcgaaggc
     8521 aatttgagag gagaaagtcg ctactttagc aaaatcatat tcctcctgcg atataacaag
     8581 acacatatca gcctgtttgg ccatttctga atgctgattt ccggtaatca gcaaggtagg
     8641 ggcctctaca tgcttcaaat aggtcattat tcgttcataa tgatggatgc gaccgctgta
     8701 gctaataaaa atggcgcaat cctcgctagt caaattggct gcattccaag aagaatcact
     8761 gtattcttca gccatgatca aaaacttatt caacttgacc attttattct ggaatttcct
     8821 tgccgtgatt tgagaatcac cttttgcaaa aagaaagatt cgctgtgcct tgttcaatac
     8881 tttaccgatt tgatctaaat cttcatgttc aatctgcaat tgtgcttttt ttattgactg
     8941 gatcgataaa tccgccattt tctttgcaat ttgctgagtg ctatcttcac ttgaaaaagg
     9001 aaaattagga tcaatcgttt gattgaactg agcaatcaat tgatgaactt cccgccccag
     9061 ctcgaaacgg aattctttaa atccgctgaa tcccattttt ttgcataaac gaatgatagc
     9121 tgaatgggaa gtatacgtag cttttgctaa ttcttcaatc gtcatgtaga ctgcagcttc
     9181 gatattccta cggatgtaat cgacaatccg tttctctgtc gtggtaaaca actcttgttt
     9241 tgttagtttt tcttcaataa gcatgatctg acctccaaat tctatcttac tgataaacac
     9301 ttggttccgc tactatcttt ttgattttta tccttacttt gaacattaat ttcaccaaac
     9361 cgaatgataa caagtgtttt tctgaacttt tttaccagaa tagctattat aaatactttc
     9421 tgcgcttttc taggatatac tgaaaacatc aataaaagga ggcaccactc atgcttacaa
     9481 tcgcttatct tggcaatgga aagagtacaa atcgctatca tttgcctttt tcaagaaaac
     9541 ttacgaacaa aatcaatatc aaaaaaattt tttccccatc tgaaccagtg tggaaaacat
     9601 tcgaagacat cgaatatact gatcagctgt ctgatctttg ggatgatcct gagatccaat
     9661 tagttgtcat cactacacct agtcaatttc attatgaata tggaaaaatg gcactcgaac
     9721 atggtaagaa tgtactagta gagaaacctt ttgcagaaac gtcaaaggaa gcagaagagt
     9781 tatttgcatt agcaaaagaa aaaggtctat ttatccaatg ttaccaaaac agacggttcg
     9841 attctgactt cttgacagtc caaaaagtaa tcgaaagcgg aaaattaggg gatattttag
     9901 aagtagaaat gcattatgat tatttcagac cggaagtacc agaaaatacg catgaatttt
     9961 ctactgcgaa cagctattta tatggacatg cttgtcatac agtagatcaa gttctttctt
    10021 atttcggcga accagatgat atcagctatg atgtacgtca acttttaggg aaaggacgaa
    10081 tgaatgatta cttcgatctg gattttttct atggaaatat gaaaatttcg ataaaatcca
    10141 gttatttccg catcaaagaa cgtccaagtt tcgtcgttta cggaaaaaaa gggatgttcg
    10201 tgaaacaaac aaaagatcga caagaagaac atctgaaaat gttttacatg cctgatcatg
    10261 aagactttgg tatcgatttg cccgaacatt atggtgtatt gacctatata gatgaacaag
    10321 ggagttatca tgaagaaaaa gtaatttccg aggttggtga ttacagccgt gtctacgaag
    10381 ggatttacga gacattagtt aatggcgcag aaaaagtcat caaagatgaa gaaacaatct
    10441 tacaaatgaa aattttggaa gaaggaatca aaagcattca agaatcagaa ggaaaaatat
    10501 gacatatttg taagaatcgg gtttgttttt tgacgcataa actgtcttat tgtacgatga
    10561 aaaagtatag aactactagg aggaaccaac atgacaaatt atgacggtga aaaacacaaa
    10621 cgattaagag gttcaatggc ccctaaaaca gaagaagaag tggctttagc aggaactaat
    10681 tctggtgaaa cagaagacaa agaagaatca ttagctggca caaactctgg tgaaacagaa
    10741 aataaagaaa cagctttatc tggttcaaat tctgtagaag aggaaacaaa gcctaatgag
    10801 cccaatcact ttgaaaaaga tatcgaaaaa gcggttgaag atgtcaaaga aaaatttgaa
    10861 gaactgacct caaaaaataa acatgatcat cattgatcgg acgaaatcaa ttcataaaag
    10921 caggagacat ttggtcacct gctttttttc tgtatccatt cgatagaaat cgttcggtac
    10981 agtcgtaaaa aaaatccaac tgtaagttgt accaaacacc cctcgattta aaagcgctac
    11041 catactagaa tataagtagg aggtgaacag cgctgtgaac tacaaaatta gttatctcct
    11101 gaaaacacca agagaaatca gtctagaaaa agcagcagag atcatcgaag taaatacaag
    11161 cgaggcgatc aaacaattaa ctccttttgc cgaatatgtg gatgataccc gtatccgtat
    11221 aaagaaattc cgagcaatcg actgggcaga aaaatttttg gatattactc gatttgaagc
    11281 tgtgtatacc gaacaagagc gaagagatct tctttatttg atggtttatt cgactgcaac
    11341 gtctgtttct gtttttcatt ttcaagattt tcttcaggtt tctaaaggga cagtcctgtc
    11401 agatatcaaa aagataagaa atgaattaga agtggaagat tgtcagctgt cgtatactag
    11461 gaaaactggt tttaggctgc tagggtcaga agcaatatta agaagagaag caaaaaatcg
    11521 cactgctcgt ttgatgcagt cacttgctgg aagatttggg ctcacgtatt taaccgcaac
    11581 cctttccatt gattattacg ccaaagttag agatttgcta gataaaacga tttcactatc
    11641 agatcttcga ttcgcaccaa atcgaatgga tgaattagct tattatttga gtttgacaac
    11701 cattcgaatt ggaaaaacat ccatggaact ggtaaaagat gctgaactgt tggcttctct
    11761 tgcagtcact gacattagtc gacaagtatt gactggcctt ggctattccg aacatcaaaa
    11821 ttcggagatt atatttttct ctctttgctt agcatcgatc gttgaaggag atgtccagga
    11881 gcctgcactt gattttttgc tggaatgttc ttcagaagtc atccatcgca tggaattcct
    11941 gatggccgta gaatttgctt cgtttcgtga attattgatg aatgtctttt atcacttggt
    12001 ccctgcctat tttcgtattt cctattcttt ttatttacct aatgtgatga tcgatcaaat
    12061 taagcatcaa tatgcttcca tctacgaaat gacaagaaaa gcattacttc cgttagaaag
    12121 acgaataggg aagagcatcc ctgaagaaga gattggtttt ttcactatac tatttggcgg
    12181 agagatcaga aaagcggacg cagaagaaag aaatagaaaa ataagagccg tgatcgtctg
    12241 tccaagtggc attagctctt cattgatctt aaaaagtgaa cttcagcaat tattcccgat
    12301 gatctttttt acagagacta attcagctga ccgcttacac gaggttgacg aaacttctta
    12361 cgatattatt ttttctactg tacctctcac acttactgat aagaaaaagt ctctctatgt
    12421 tctgcatcca atcatgactg acttggaaaa aaatcagctg atcaatcaag tacaaagaga
    12481 ttggctgata ccaggatttt caatgccttc tgcccaagaa ttgttgtcgg cgctactgcc
    12541 ttatatcgaa ttaaaagaag gcgtagacga agaaaagctc tatcaagtat taaatcgtaa
    12601 aatgaacaaa cttttagaaa agaaggagga tcaacgtcct atgctatctg aattattaac
    12661 agaagaaatg atccaattat cggatcaacc aaaaaactgg caagaagcaa tcacatttgc
    12721 cgcacagccg ctattatcag aaaataaaat agaagaaagt tatatcaaag ctatgatcga
    12781 gcgcgtggag cagtatggtg cgtttatcca tattggcgat cacatcgcat tacctcatgc
    12841 tcgtccagaa gatggcgtaa acgaagtagg gatgtctttg ctgaaacttc aacaacctgt
    12901 ggatctgctt gataatccag cccatcccat tagtttgttc atctgtttag ctgctatcga
    12961 taatgaggca catcttcggg ctttggcaaa tttaacaaag ctactttcta ataaacaaaa
    13021 tcttcaagat ttattacacg catcaacaaa agaagaaata ttagcgatca taagaaaagg
    13081 agacgaatga tatgaaaatt tttgcagtat gtcaaagtgg attaggaaca agttttatgg
    13141 tagagatgaa catcaaacaa gcacttgcag atcttggagc agatgtggac gagtttgaag
    13201 taagtcatac ggatgttgga agtgtatcta gcgatatggc tgattatttc ttcattgagc
    13261 attctttgaa agatacgcta acttctatac ctgatgaaaa gctcgtacct ttgcaatcga
    13321 tcattgacag cgaagaagta aaagaaaaag taacgaagat cttgtcaaaa gaataaaaat
    13381 gattcaaggg aggaactatc aatggataaa ttgttgaatt tgtttgtgtc aatcgtcagt
    13441 cagccctcca tcctcgtttc tttgattgca ctgcttggtc tcgttcttca gaagaagaaa
    13501 ttttcagaag tgatccaagg aactatcaaa acatttgtcg gattcctagt actgatcggc
    13561 ggtgctggtt taatcagtaa ttcactaacc ccttttgctt cgatgttcca aacagctctg
    13621 catactcaag gagtagttcc tagcaatgaa gcagtagtgg cgattgcttt gcaacaatac
    13681 ggtacaccta ctgctttgat catgctagta gggatgatcg tgaatatttt acttgctaga
    13741 ttcacaagat ataaattcat ttttcttaca ggtcaagcaa tgctttatgt ttcttgtcta
    13801 acagcagtca ttttagtaag cgcggggttt actgcaaatt tgtggatgat cttattaggc
    13861 ggggtcttcg aaggaacact attaactatt acgcctgctc tttgtcaacc gtttatgaag
    13921 aacatcactg gaaacaatca agttgcgtta gggcataccg gaaatatcgg ttatgcttct
    13981 agtggatgga tcggaaagtt ttttggaaac aaagaccgtt ctacagagga cataaagatt
    14041 cctaaaagtt tcagcttttt gcgagattct actgtatcga tcatgctctt gatgtcgatc
    14101 gtatatgtcg cacttgcttt gttagctggt cctggatttg ttgaaagaga actaagcaat
    14161 ggagacaacg ctgtaatctt tgcactgatc caagctggaa cattcacagc tgggtttgtt
    14221 atcgtccttc aaggggttcg aatggtgttg gcagaaatcg ttccagcatt ccaagggatt
    14281 gctaaaaaac tcgtacccga ttctaaacca gcattagatg ttcctatcgt ttttccatat
    14341 gcacctaatg ctgtattaat tggctttttt gttagcttct ttgtaggaac actatccatg
    14401 tttgctatga taggcttgca aactacagtt atcattcctg gggtagtagg acatttcttc
    14461 tgcggagctg cttctggtgt cttcggcaac tcgactggcg gtagaagagg ggcggtatta
    14521 ggttctgctt tcaatagctt attgattagt tggctgccat tatttatcct tccagtatta
    14581 ggagatctta aaatcgcttc ttctacattt gccgatactg attatctagt accaggactt
    14641 gtacttggga aactaggaga atacggtcaa atagccttga cagttggaat catcgctttc
    14701 tttttacttg tcgtattatc tagctttctt tttgccggaa aaagaacaaa cattgtttct
    14761 aaagaacaga attaagcaaa cttcaaaata aaaattgctt ggacaatatc agaagaagat
    14821 ttaaagtctt ctgatatgtc gcaagcagtt ttttgtttta tgcatttatt tttgaatagc
    14881 ttgttaagac aagtggctgt tttgctagaa gaggataact tttaccattc attttcactg
    14941 ttccgcctgt tctatcagaa gtaatcacga tcttatcttt tgttttttca agcgaaatct
    15001 ttccatcttt gataggtagc gtacaattca gtgtttcaaa tgaatctaga ttaggttgta
    15061 tcataaattc ttcataacct ggctttgttg gatacaaacc gataaaatag cgtcctaaaa
    15121 gatagattgg gcttgctccc catgcatggc ataagctttt tccataagga tctccataca
    15181 tactgtactt ttcatttcct gtttgagtag ggtcgtattc ttcccagaaa gttaccgcat
    15241 catgagataa catccctccc caataatcaa gaatcgtttg gtagacgata ttcgtctgtc
    15301 cgagtttgca taaagcatct tgttcataga atttgaaata tggagtagta atctgtgtga
    15361 tagtgtcatt caataagaca ttctttaaga tcgattgctg ttggtctttg ttagcaaaat
    15421 cgaataaaac agcgaaaata ttcgcatgac gcgtcacatt tcttctccca gattcatagc
    15481 aatcgataaa agcgccaagt tcttcatccc aaaagtagtc aaataggttt tgtttcaata
    15541 gttgatattt ttcttgatag agatgagtat gataattttc gttctctcca tatgtttcag
    15601 ctaagatatc tccgcaagtg atcatggaat gataggcttt cagtaataag acttgttctg
    15661 cagcaagcgt tccttcttta tccatttcag accaatcaat aaatatccaa tcttttgctc
    15721 tcccataaat aaaccctaat tcattcgttt gctccatcaa gtatctcatt aatgattgca
    15781 tcttagggta gacgatgcgt aaaaattcat agtctttcgt catcatatag tggttttcga
    15841 taccaatcaa ccacaagatc gaatagtcaa cgatagtatt catatgctgc ttgatttcat
    15901 tttgtcctct taatgctaat atcgttcgtt tattgatttc ctcgtcaaaa aaaagatatt
    15961 gattgatcat gaatgattga tacgcatctc cagaccaaat ccagcgatct cgtttgatac
    16021 cgtcgataaa aaacaaatca ctacataact tgaaggcttc ttctgatact gaccagatct
    16081 gattcagtaa agaagaatcg cttgaaaaag cagcttgttt cgaaagagat aaaaattcat
    16141 gaaaaacttg aagttgaacc attccagtag aaacctctgg tataaacaaa tagcgaaaag
    16201 cgcgtttacg agctttcatt tttcttgtca ttcctatttg cttatagtag caataatcga
    16261 tatccatagc ttctacatcg gactctccat aacatagagt gatagattcg atatggtcat
    16321 ccagagatat tagttctaca cagccattca ctgctcgccc aaaatcgaat aagattcctc
    16381 cgccaatctc cgtttcagtg actggatgga cgcattcttt ttgataatag atttgatttg
    16441 gatcttttgc gatatcgaca taaagcggac tccagccagc tggatattct tcaataaaat
    16501 tacttgctgt ccagccaata tctgatttta cttcgtctcc ctcgataaac atcgctggta
    16561 gccctgatgt atgtcctaca aagatacgaa tttttgcttt accagctgga caagtcagcc
    16621 attttccaca tggatgtttt tgtccattaa tttctacata cccaactcct tggatcgtca
    16681 ctttaaacat ggaaggttga tcaagaaagt aatatctctt aaacttgacg ttccgatggc
    16741 aatcatccat gtaccagtac gctggccagt caaaacctcg ttcttcccgt tggaaatttt
    16801 gtagcagtcc atggcggatc tcaaagtctc caggatacca aagccatcta tctttatgtt
    16861 tttcatttgt cattttacac gacctccatc aaatttattt tgttgtcaat tgttctttta
    16921 tttcttcatc tactcttatc actgtctttc tattcgtcct gtgtactact agaaggctaa
    16981 taagcgcgct aatcaacagc aagatactgc cattgacgaa aacagcaagt gttttactcc
    17041 caagagaagc aaaagagaga aagagcaaac catatggtgc aatgaaagta cctaaattag
    17101 cgccaaccaa taagattgaa gtattgaaac tctccgcgta cttgggcgct tggatactta
    17161 cttggttgaa taagtaagga atgaataacg aaaaagaaat tcctgttagg atcgcaccaa
    17221 acccaatcac atacaggttt atcacagtgg caatcaatac aaagccgatt cccatcagta
    17281 gtaaagaaag tgtaagcata tagcgatgtg cttttgtctg aagccagcca aaacaaactc
    17341 cggcagccat acttgccaat tgcatgattg ttaagatatt cgctgcatta gtttcgtttc
    17401 ctagagaata agtcgtcagg actgtggcta atttaatcga tacagcatta tataccacga
    17461 tgaccagcaa gagaaaaata ccataactaa tcattttagg attcgttttg attacttgtc
    17521 ttgttggggc tgtgccatgc gattgaagtt cagttgtttc aggtgtagga acaaacaaaa
    17581 caaacaagac aagaataggt aagctgatgg cgtagactaa aaaggtatat tgccaaccga
    17641 actgtactag ctgtcctgca acaaatgtca ttaaagctgc accaaatcct tgaaatgcac
    17701 tttgaaaacc aatcatagag gctcgttcat ctccgtgata aaaggcagca atcatgctga
    17761 ctgccaaaga attgatcaat ccaaagccaa tacctagtcc agctcttgaa gctaagacaa
    17821 tactgtaact ttgtgagaag agggggagga taccacaaat cgatgcaatc actaatccga
    17881 tgatcacagt ttgtttttgt ccgattttcc tagcaataaa actactaatc aaaacaaaaa
    17941 tagtgaccat aaaagaggga gcagtcgtta ataattcaac agcagataaa gaacgcgtgg
    18001 cataagtagc tgacatactg ggaattgcag acgtgactgc accagcagaa gtcaaaataa
    18061 gagagactga cagcagagaa aatttcatca tctttttatt cataagcatg ttcctttcat
    18121 ttttgtaagc gcttatcaaa aatatagtat gaagtggtta ttctctcaat agcggaatca
    18181 caggaagttc atgtcccaac catagaaagt ctttatgaaa ccaaaaatag ctttgcatat
    18241 aatagcagta cagcgtgaac taataagcga aagaaaccgc ttacaaaaag gagggagtca
    18301 attggaacta gctgaattag acattttttt tgcgtaacga agaatcaatc gaaactctgc
    18361 agcgtgaaca aggaatgaat atcaatgatt taaacttagc ttaccatctt cagacaccgc
    18421 caaaaatcca ccgaggggat ctatttcaac agggaaatat tcatatcagt aaacaccgtc
    18481 gatatgcgga agttcccgag catacacatg attttgttga aatcaattat atgtatagcg
    18541 gtacatgttt acaaattatt aatggagaaa ggatagagat ggaagaaagt gacgtggtga
    18601 tcattgataa agaagctact caaagaatcg aatacacagg gcaagaggat attttaatca
    18661 acatcttatt aaaagaagaa accttatcta cagagatatt gaatcatttg gcaagcacaa
    18721 ataatctact ttcttctttt ttgataaatg catcaaaaga aaaaggggaa catacacaat
    18781 tcattcattt tcgcggcgga aaaacaagtc gtttgacgca tttgattgaa aatatgctgt
    18841 tgatttattt tgatacatac agtcataaga tccgttcgtt aaatctctat acatcactat
    18901 tactgatcga attgactcat ttattagaac aacaaagctt ttcatcagca gatggatgcg
    18961 cagaaaatga gatgcttaga gtattagatt atatcgataa gcattttact tcttgtacgt
    19021 taaaagaaac agcagctcat tttggctaca atccggtcta tcttagtaat aaattgaaaa
    19081 tgtattttga tgcttccttt caagaactgg ttctaaagaa aaaattcgac tttgcgttgg
    19141 aactgattca agaaacgagt ttttcgttgg aagaaatcgc taatcgtatc ggatataaac
    19201 aaacgacttc tttattcaaa ttattcaaga agtattcaac atgtactccc aatgaatgga
    19261 gaaataaaca ttaagaagaa aatctttcac taaagcatat gaaggaaaat tgttttaggt
    19321 gcgattgctg tcttattata tcgttgcagc gaattttttt ggatttattt tcagttctaa
    19381 acttttggtt gtattatcag gg
//
LOCUS       DACEXQ010000026        18895 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.026, whole genome shotgun sequence.
ACCESSION   DACEXQ010000026 DACEXQ010000000
VERSION     DACEXQ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.026"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(21..251)
                     /locus_tag="IXH04_000743"
     CDS             complement(21..251)
                     /locus_tag="IXH04_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851374.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            complement(687..1253)
                     /locus_tag="IXH04_000744"
     CDS             complement(687..1253)
                     /locus_tag="IXH04_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ1851375.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1253..2209)
                     /locus_tag="IXH04_000745"
     CDS             complement(1253..2209)
                     /locus_tag="IXH04_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAQ1851376.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2209..2865)
                     /locus_tag="IXH04_000746"
     CDS             complement(2209..2865)
                     /locus_tag="IXH04_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAQ1851377.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3235..5253
                     /locus_tag="IXH04_000747"
     CDS             3235..5253
                     /locus_tag="IXH04_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1851378.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            5680..8094
                     /locus_tag="IXH04_000748"
     CDS             5680..8094
                     /locus_tag="IXH04_000748"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAQ1851379.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8442..9995
                     /locus_tag="IXH04_000749"
     CDS             8442..9995
                     /locus_tag="IXH04_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAQ1851380.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10376..12160)
                     /locus_tag="IXH04_000750"
     CDS             complement(10376..12160)
                     /locus_tag="IXH04_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1851381.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGFLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(12175..13896)
                     /locus_tag="IXH04_000751"
     CDS             complement(12175..13896)
                     /locus_tag="IXH04_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1851382.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13996..14757
                     /locus_tag="IXH04_000752"
     CDS             13996..14757
                     /locus_tag="IXH04_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1851383.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            15724..15992
                     /locus_tag="IXH04_000753"
                     /pseudo
     CDS             15724..15992
                     /locus_tag="IXH04_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            16664..>18895
                     /locus_tag="IXH04_000754"
     CDS             16664..>18895
                     /locus_tag="IXH04_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851384.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
BASE COUNT         6206 a         3352 c         3786 g         5551 t
ORIGIN      
        1 ctcattaaaa agcataacca ttactccgat ctgtttttaa aattagtttc tacctctaat
       61 atatatcgag tttttatttt gccttcagag aatactgttt ctttttttgc agttacaggt
      121 tgtttatttt cggaaaaagc taatatagct aagattgaaa catccatctg gaatttatct
      181 tttttactgc tttgatcata aaagtctgca tattcatcac tgatattttt tctaataaat
      241 tcttccatca taaaaatcac ctcaaaacga ttatacgaac ttacgttctt gttgtaaagc
      301 gatatttgag gggcaaaaaa ggggcataat ttgactgata ttaagttagc tattgatata
      361 tgaatgacat acaaaaatat aaaatctttt aaaaatgctt atatatcaat gtttttgcca
      421 ttcttttata tatctgttat atgtcaaaaa aatgtgttca atttcaattt gtatttgaat
      481 aaagaggttg tgaaaaagct gtcctgcatc aagtaataag aacagccaat caaaaacagc
      541 ttctcatgtt tttgatttgg ttggacttat taccgcagat ataagctttt gagcaccgtg
      601 aataaagagg ttatgaaatc aacgctctaa cctgagtatt agagaaaaaa acgcaaaaca
      661 tatcatttat gttttgcgtt ttttatttat ctttttttct caatacaata taaaataggc
      721 ggctggttct tttggttgat aaaccgataa ttcagcacat tatatgtttc ctgtggcagt
      781 ttggaacaaa aagaatcaac catatccagt tctttttctc caccttcatg accatagtag
      841 acgacaagga tcaatcgtcc gcgaggcaca agtcgtttca atatttcttc catagctgtg
      901 cgtgtagtct ctggcatagt gatgattgct ttatcacttt ttgggagata ccctagattg
      961 aaaatccctg ctttgagatt ttgttgttca gctatcactt ctcctaacgt ttcgtggcct
     1021 tgtagaaaca atgagacacg ttcttctaac tgacgctgtt ctagcctttc acgagtagca
     1081 tcgattgctt gttgctggat atcaaaacta tagacatgac cagtttttcc gatgtgttct
     1141 gctagaaaaa ccgtatcgtg gccattgccc atcgttgcat ctacgacgtg atcccctggt
     1201 tgcaagattt cttgcagtaa tgtatggctg aaatgcaaag cagtttgcaa catcagacgc
     1261 tcaccttttt ccgtacatca tattttcctt ggaagctatc tctgcgaaga agttcttcat
     1321 caattgcatt caagacttcc cattttttca agctccacat tggtccaatc aaggaatccc
     1381 acggcgcatc ccctgtcaaa cgatggatga tgatctcttt tggtatcatt tccagctggt
     1441 cgcaaatgac attgacatag tcaggacggc tcattaattg aaggcgacct tcatgatagt
     1501 cccgcaacat tctcgttttg cgcatcagat gcatcaaatg gagcttgatt ccttggatat
     1561 ctgaatctaa aatggtgcgt ctgacatttt ctttcatcat ttctaaactt tcgcctggta
     1621 atccgttgat caaatgcgta caaacacgga tattatgttt gcgtaattta gccacgccat
     1681 ctaaataagt ttgataatca tgtgctcggt tgatcagttt tgaagtttct tcaaaagttg
     1741 tttgcaatcc aagttctacc cataaataca tccgctcgtt cagttcagca aggtaatcaa
     1801 ctacttcatc tggcagacaa tccggtcgtg taccgactga taatcctacg acacctggta
     1861 aattgactac ttgctcaaat cgttctttga ttatttcaac tggcgcatgg gtgtttgtaa
     1921 aattttggaa ataaacgatg tattgttgga catgaggcca tttttgatgc atcatgtcga
     1981 tttctttctg gaattggatt ggaagagggt cttcaggtgc aacgatcatg tctcccgaac
     2041 cagatacact gcaaaaagta caaccgccat gggcaacagt cccatctcga ttcggacagt
     2101 caaatcctcc atcgatcggc accttgaaaa ttttcccgcc aaactcattt cgtaaagcgt
     2161 aattccaaga atgatatctt ttatttcctt ctgaataagg aaaagtcatt aaaagtcctt
     2221 ctttctgtaa gagttatcta tttgttttct tttttctcta tacactggat aagtcaggca
     2281 cagccaagaa agactgagta agtagccgcc gatgatatca gtaggaaaat gaacgcccag
     2341 ataaatgcgg cttatcccga cacttactat aataaacatc atcagcaatt gaagcaatgt
     2401 gcgtaaggtt cttttttgaa caaaagctgg aagcagaaat agtaaagaac cgtaaagaat
     2461 catactgcct gctgcatgtc cactagggaa actgtaactt gtttcaacta ctaagtgcgc
     2521 caatgaagga cgctctcgca tgaaaaatag tttgatcacc gggttgatga ttccagcaat
     2581 tacaataaat ccagaaacga accaaaatgt ttctgccctt tttttcttat accacatcca
     2641 taaagccgca caagcaaata aaatgatgac ggtaagagga ttaccgaatt tagtcataaa
     2701 gagttggtag ctgttcagat caggataaga agctctgaca atgcctgtaa accactgatc
     2761 gaaaccagaa agccacgaca gatggaactt tacgatccag cccaaaatga aaaacaggac
     2821 aagaaagaag ctaccagcca cctgaaatga tgttttatat ttcatacaat tcctcctttg
     2881 aagcaatgac ttattatagc atttttggtc cagcgaaaaa aacggtaaag agataaaacc
     2941 aatgtatgat tcctctttga ataaccaata ttcttataaa aaaaataact gtaatcggaa
     3001 agacattttt tggtaatgca aatgaaatgt tagggaaata aaatcagtgt ttttcgaaat
     3061 ccttttaaac aagaacgtag acgtaggaag aacttgaaaa tgaacagaag ttgttcatta
     3121 cggtaaatta caatatgatt acaaaaaaca acaaagctct taaataactt ttaataaata
     3181 ttaagagaag ttataattat taataataga taaattgaag ttggaggata gaagatggaa
     3241 aacattgcaa gaaaagaacg gagacgttta gaacagacca aacgttttcg aaaagcaaaa
     3301 cgaggggcag caatcgtggg aactgcgatg gtaggctgtt ctgtcgctgc cccattagta
     3361 cagccggttc ctgttcaagc ggatgaaaca cctgttcagt tcggcgcaag aatcaataca
     3421 gcggcattta tcacagaaat tgctacttat gctcaaccaa tcgctcaagc caacgatcta
     3481 tatgcatcag tgatgatcgc acaagcagtt gtagaaagcg gttggggcag tagtgcgtta
     3541 tctcaagcac cttattataa tctttttggg ataaaaggca gctatcaagg acaaaccgtc
     3601 tacatggata cgcttgaatt tttaaataat caatgggtaa ataaaaagga gcctttccga
     3661 caatatcctt cttttgctga atctttcaat gacaatgcct atgtacttag aaatacttca
     3721 tttggcaacg gttattacta tgccggtact tggaagagca acacgaaatc ctatacagat
     3781 gcgacagctt gtttgacagg gcgttatgcg actgatcctg gttacgcagg caaattaaat
     3841 aatatcatcg caacttacgg attgacccaa tatgatacac cagctagcgg aaacgcaggc
     3901 ggtggagcga ctacaggtgg caataatact ggaagtgcag gtagtactgg cggttcaagc
     3961 aattctggta atacaaactc aggttcagca acttcgacca cttatactgt ccaatctggt
     4021 gattctgttt ggggaatctc aaataaattc ggtatcacaa tggatcaatt gatccaatgg
     4081 aacaacatcc aaaacaattt catttatcca ggacaaaaac tgacaatcaa aggtagccag
     4141 gaaaatggct cttcgacgaa taattccggt aacaatacca actcttcagg aaatgctggt
     4201 acatcaaacg gaggacaaac aactggtgcg aaatacactg ttcaatctgg tgattctgtt
     4261 tggaagatct caaatgatca cggaattacg atggctcaat tgattgaatg gaacaatatc
     4321 aagaacaatt tcgtttatcc aggacaacag ctaatcgtat caaatggcgg ttcttctaat
     4381 gctggagcaa atactggctc aaatactggg aatacaagtg gctctagttc gtccagctca
     4441 agttcttcta atacagcttc aggaacgaaa tatacagtta aagccggaga ctctgtttgg
     4501 agcgtctcaa ataaatacgg aattacgatg gctcagctaa tccaatggaa caatatccaa
     4561 aataacttta tctacccagg acaacaactg actgtttcaa acagtggatc aacaaacagc
     4621 acgtcaggta atacaggttc gtcaaccaat acaaataaca gcagttcagt taatactggc
     4681 agcagttcgt ctcaaggcac ttataccgtg aaagcaggtg aatctgtttg gagcgtggca
     4741 aataaaaacg gcatcacgat ggatcagtta atcgaatgga ataatatcaa aaacaacttc
     4801 atttatccag gacagcaatt gatcgttaaa ggtggaactt cggcaaatac aaatacaggt
     4861 tcaactactt ctgcagcgaa accaaataca ccgaatacgt ctgctacgac aagtacatca
     4921 tccactggta atacgatgta taccgtgaaa gcgggcgagt cagtttggag cgtagcgaac
     4981 aaacatcata tcacgatgga tcagttaatc gaatggaata atatcaaaaa taatttcatc
     5041 tatccaggac agcaagtgat tgtgaagaaa ggttcatctc aatcaacaaa ccaacaatca
     5101 gttactggtt cgaaaaccta tactgtgaaa tctggagaat ctgtttggag tgttgctgac
     5161 agtcatggaa ttacaatggc tcaactaatc gaatggaaca atattaagaa caactttatt
     5221 tatccaggtc aaacgttgat tgtagctaaa taaaatagct gttcgaagaa ataacgattg
     5281 aattccacaa aaatatttgt tactataagg ctacttaaat atgacaacac ttgaaaagga
     5341 agtagtaaaa agactgaagt ttcagagagc gcatggctgg tgagaatgtg cacggatgct
     5401 tttgaactcg cctttgagtt gcccttgctg aatacccgag taagcaagcg gcggtgacgg
     5461 ccgttacaac gttcgaggcc ttttcactaa ggtaaaatta ggtggtaccg cgtagattac
     5521 gtcctataaa aagagcaatc tttttatagg atttttttat ttcttttgtg aagtttgaga
     5581 aaacaaagca aaaggaaata aaaataaaca gattctgccg gttttttcgt tatatttata
     5641 atagaaacaa gtgttttcaa tagaacagga ggacagcaaa tgagttacaa tcataaagaa
     5701 attgagaaaa aatggcaaaa atactgggca aaaacgaata ctttcaacac ccatgatgat
     5761 cctgaaaagc caaaatttta cgcactggat atgtttccct atccatccgg tcaagggcta
     5821 catgtagggc atcctgaagg ttatacggca acggatatcc tttcacgtgt gaaaagaagt
     5881 caaggataca acgtgctgca tccaatgggg tgggatgcat tcggtctgcc tgctgaacaa
     5941 tatgcgctag atacaggaaa cgatccagct gagtttaccc aaaaaaatat cgaaacattc
     6001 cgacgccaaa tcaattcttt aggatttagt tatgactgga atcgcgaagt gaatacaact
     6061 gatccggagt attataaatg gacacaatgg atctttacga agctttatga aaaaggtcta
     6121 gcttatgaag cagaagtagc ggtaaactgg gtacctgaat taggaacagt tatttcaaat
     6181 gaagaagtga tcgatgggaa aagtgaacgt ggtggctatg atgtagtcag aaaaccaatg
     6241 cgccaatgga tgctgaaaat cactgcttat gctgatcgct tattggatga tctagaatta
     6301 gttgattggc cagaaagcat taaagaaatg caaagaaatt ggatcggtcg ttcagtagga
     6361 gcaaatattg aatttaaagt ggctggtacg gataaatcct ataccgtatt tacgacacga
     6421 ccagatactc tattcggtgc aacttactcg gttcttgcac ctgaactaga tcttgttaga
     6481 gaaattacga cccctgaaca aaaagctgct gtagaagctt atatcgaaga gacagctaaa
     6541 aaatcagatc taaagagaac ggatcttgca aaagaaaaaa caggagtatt tactggtgct
     6601 tatgcaatca atccagttaa tggcaaggaa atcccaatct ggatcgccga ttatgtatta
     6661 tcttcatacg gcacaggagc catcatggct gttccagcac atgatgaacg tgactttgaa
     6721 ttcgctcaaa ctttcggact agaaattctt ccagttatcg aaggtggaga tgttgaaaaa
     6781 gctgcttaca cagaagatgg tacgcacatc aactctgaat ttctagacgg catgaacaaa
     6841 caagaagcaa tcgataaaat gaatgaatgg ctagaagaaa atggcgtagg gaaaaaagaa
     6901 gtcagctatc gcttacgtga ctggctattc tcccgtcaac gttactgggg tgaaccaatc
     6961 ccaatcatcc attgggagga cggaacagtc acagctgtcc cagaagaaga gctaccatta
     7021 cggttgccta agacaaaaaa catcaagcca agcggtactg gagaatctcc attagcaaat
     7081 atcgaggagt gggtcaatgt cgttgatcct gtaacaggta aaaaaggccg tcgcgaaacg
     7141 aatacaatgc cacaatgggc aggaagctca tggtattacc tgcgttatat cgaccctcat
     7201 aacaaaaaag aactagcaga ttatgaaaag ttgaagcgtt ggctaccagt tgacatctac
     7261 attggtggag cagaacatgc ggtacttcat ttactatatg cacgattctg gcataaattc
     7321 ttatacgata tcggtgttgt tcctacaaaa gaaccattcc aaaaactata caatcaagga
     7381 atgatcttag gcgaaaacaa cgaaaaaatg tcaaaatctc gtggtaatgt agtcaatcct
     7441 gatgatgtgg tcgaaactta cggtgcagat acactgcgca tgtacgaaat gttcatgggg
     7501 ccattagatg cttcgattgc ttggagcgaa aatggcttag aaggaagccg taaattcttg
     7561 gatcgtgtat ggcgtttgat cgttgatgag aacaataaaa tgcgtgaccg tatcactaca
     7621 ttgaatgatg gcaagcttga taaagtgtat catcaaacag tgaaaaaagt gacagaagac
     7681 tatgaaaatc ttcatttcaa tacagcaatc tcccaattga tggtctttat caatgaagcc
     7741 tataaagtgg acgctctgcc ttatgaatat atcgaaggat ttgttcaact actcgcaccg
     7801 attgctcctc acatgggtga agaactatgg gcaatccttg gaaatgatgg tggtatttct
     7861 tacgcaccat ggccaacata cgatgaagca gcacttgttg aagacgaagt agaagttgtt
     7921 ttccaagtaa acggtaaagt tcgtgcgaaa agcaatgttc cccgagactt aggaaaagat
     7981 gagctagaaa aagcagcatt agccaatgaa atagtgcaag aatatattga aggcaaaaca
     8041 gtcagaaaag ttattgcggt accaaacaag ttagtcaata tcgttgctaa ttaattggct
     8101 ctttgtcaat aaggactgat gagttgtgca aaatcaaatc tgggccagaa tgaacttcat
     8161 tctgtctcag attttttgtt atttcaaatt gattaattga ttgatcaaaa aggttttgat
     8221 tttcatgttc tcaaacacgt tcaatgtctt tttcttctga atggtcttgt aagagatctc
     8281 tgtaatcatc aaatttttgt ctattaatct tagtattttt tgatgtataa actacctgaa
     8341 attgagagta cataccaaat tcataaattt tttattagtg ggactagtga ttgtatggat
     8401 tttgacgcat aatgaatgaa gtatgagaaa aggagagaca aatggatcaa gaactattta
     8461 ttatcgaagt attatcagct aaacaaaaaa ttatcgagaa agcgaaaaaa agagcgaaaa
     8521 gaataatgtt caagctttta agcttagtat ttttctctgg tttgcttttt ttcggagctg
     8581 tatcgttcct atatcgccat atgagtgcag tagtccgttc agctgaaggg atgcaaaaat
     8641 taaataattt gtatcacttt tcgtggggag ttggcggaat aacaattgtt gtgcttattg
     8701 tttttcttct acttatttgg ctccgttacc atcaattgtc gcgtcatttc gatcaatggc
     8761 ttagtcaaag agcacaacag tttttacata ataaaccaac ttctcaagat gatgactatc
     8821 tttattttga gaagacttcg acgagtcaag cttttcggat acaaaaaagt gattgccagt
     8881 tattgctgag atcacttgat gatttaccta tctacttagg cgaaagcaaa tcaaaattcg
     8941 gaatgaacac attccatatt tttttactag aaaacgaacc cagatgggaa aaaagccagt
     9001 cttcgcctgg tggatacctg acaactaaga tgagtacatt agtcactggt tttttcctca
     9061 ttcttttagc tggaacaatc tattatggga gatacactca gccaaaaatg tcttctcata
     9121 taaatgctgc ttctcacatg acaatgaact caactaacga tacgcatata caaaagacaa
     9181 atgaagggtt gttagctgat cctaagcaaa aggtaaattt gacaacacaa aagataaatg
     9241 atctcaaact tgatacagag acgcactctc tctatatgac aacagatagt ggtgctaatt
     9301 gggtatttgt gccgattgat tcgcaatggt tgcgttttgg cgaatacacc ctgacaacgg
     9361 ggctgattcc tgaaggttat tggatggaca acacatatga tatttccgcc gatttctcat
     9421 ggtttatcta ttcttctggg gatgatgtac gtctgctgac ttcaagagat aatggtaaaa
     9481 catggcaaac gtctattatc acccaatcag ttcagccgat acgttatcga aaagcggtat
     9541 tttatggcaa cgatggtcta tttatctgta catcagccag tgggatgtct gcagaaactt
     9601 tgtacattta cactactatg gatggcggaa tcacttggat aaaaagtgga agtacaatga
     9661 tcaatcagcc agtacaaaat gtcagcttcg ttacaacttc tttaggtttt gtttcgacac
     9721 gtgaaagttt gttttataca aacaacggag gacgctcatt caaagaatca gttctgtcga
     9781 ttccggaaaa ctatcagaca ggggggatag atctttttca atcaccaaat gaagttactc
     9841 aaataagcgc gaatacgtta gagacaaaat tttatttgtt gaaaaaagga ccaatcgatg
     9901 cagggaaaat gtttgcctgc ctcttccgtt caactgataa cggggaaaca tggcaattcg
     9961 ttcagcaact atcgcaagta gaacaaggag attaaagaag agagattgtg agcctgacgt
    10021 tcagcaccga gtcacaagaa acaaattcaa aaaacagctt ctcatatttg ttgaatagtg
    10081 taactcgtga ttcggagttg acaagagcaa caagaagcga ttttgaagta tagcttctcg
    10141 tattttacaa gatcgtgtct tattgccgat aggttgtctc ttgaacaccg tttattcgta
    10201 aaaagggatt gtgacaagtc ttttgtcact acttaccgaa cgaaactgaa ggagctatcc
    10261 aaaaagtatc attagtatta ttctttgtgt ttctagtggc accactagta catttaaaaa
    10321 agctatcgat catggactaa tcatacatga tcgatagctt tttagataat gtttgttacg
    10381 ctatctgaaa ttgactatta taaagactgg cataaagcgt tggtgattgg agtagttcag
    10441 aatgtgttcc tttttcgacg atatcgccat tcttcattac aaggatcatg tctgcatttt
    10501 caatcgttga caaacggtgt gcaatcacaa aacttgtcct atcttttgtc acagcttcca
    10561 ttgcttgctg aatcaatgct tctgttcgtg tatctacgct agaagttgct tcatctaaaa
    10621 taacgacagg aggattcgct agaatgactc gagcaattgt caataattgt tgctgtcctt
    10681 gagataacgt gccgttttcg ctagaaatga tcgtatcata tccgtctggc agtgttcgaa
    10741 taaagtgatc acattgtgcg atttcagccg cttcgataat ttgttctctt gtggcatcca
    10801 ttttcccgta ggcgatatta tcagctaccg ttccttcaaa cagccaggta ttttgtaaaa
    10861 ccatcccaaa agaacgacgt agttctccac gtgaaagctt ggtaatatca agaccgtcga
    10921 attcgatctt tcctttattc agttcataaa aacgcatcag cagattgact aaagtcgttt
    10981 ttcctgcacc agttggacca acgatcgcta ccgtttgttt tggctttact gaaaaatcga
    11041 cttgattcat caatatttta tctgatgtgt aaccaaactg tacatttttg aaagctattt
    11101 ttcctttcgg ttgttctaaa agtcttgcat ctggaaggtc tggaatttca tctgtttcat
    11161 ctaatatctc aaatatccta tcgattgctg ccattgcgga ttggatcgag ttgatcacat
    11221 aagaagcggt agaaattggt tcagaaatct gattgatgta ctgaagataa gcttgtaaga
    11281 agccgatcgt cacaccacct gatagaacaa gcatagcacc gaataccgca cttatgatga
    11341 aggcgagttg attaatgaaa cgaatggccg gatagatagc acagttcata aattgagcac
    11401 gtttaaaagc gttgtattgc tttttattta cttcgtgtat agcagcttct gcatgagctt
    11461 gctgattgaa tgttttgacc actagattac cagctaagat ttcttccatt cgattattta
    11521 aaaaacccag ttcatactga ttctgttcag aaatctgttt gtttttgttc gcaatcttag
    11581 ttgtaacaat tgaactgcca acgatcagca ataagacgag caaagtaagc ttgctttcga
    11641 tataaaacag catcagacca gcagcagcta ttgttacgac agaagtaaaa aattgattga
    11701 tacctgtcaa taacacttga gaaagttgat tgagaccggt agttgtccga ctaatgatgt
    11761 cacctacttg atgattgtca aaaaacgaca tcggtaatgt tttgaatttt tttgtgattt
    11821 cttttcgcac cctcaatgtg atcttttcgc ttaacgctgc cattgtccgt tcctgaataa
    11881 atgaagtgat gctgcttaag actgaacagc cgatcagcaa taagacaggg aacaacaatg
    11941 cttgtttgat atcactgaaa gaaagctgtc caatacctgt ttttcttatt tctgataaga
    12001 gatcatcgat cccgattccc atgatcaagg gcatggctac gactaatagg ttcccgaata
    12061 aactgcatag aaacaatccg taaaaatatt tatgttcagg tttgatcatt ttccaaaaac
    12121 gtatcaaagc acgcgtgttc aacgtttttt tcttttccat cgtatccctt ctttctatat
    12181 tccttgtgat tttgcaaact cctgataagt ttggcaatta gacagtagtt cttcatgagt
    12241 accttgtcca acgatttttc cttcatctaa aacaatgatg ttatctgctt gttggattgt
    12301 actgaggcgt tgagcaacaa tcacgagagt tttctccgac atgtgtttat gaagggctgc
    12361 tcttaatgct gcatctgttt tatagtctaa agcggagaaa ctatcatcga atacatagat
    12421 ctctgctggt ttgatcaacg ctcgagcaat acatatccgc tgtttctggc cccctgaata
    12481 attagaaccg ccttgagcaa caaagctgtc caatccgtca ggaagggtgt ccactagttc
    12541 ttctaattga gcgatcttga tcgctttaga caactcttct gttgaggcat attcatttcc
    12601 catctgcaag ttgcttaaaa ttgtcccgct gaataagaaa gccttctgag ggacataact
    12661 gatgtgggag cggatctgtt cttgagtcat ttgagtaaga ggaataccac caagggttac
    12721 ttttcctatc gtagggtcac taagtctcaa gagaagtttc gccaaactac tttttccaga
    12781 tccggtcccg ccaacgatcg ctgttgtttt tcctttaggt attgtgaaat caatatcttc
    12841 tagaactgga tcagagattt cagtatattt aaatgtgaca tgttcaaaag cagctgcatt
    12901 tgtctgatca aaggttggtt cagctactgt tttgatcgtc tcttcctcaa tttcgatctc
    12961 tgcttgcagt atttcttcta tccgtcgtaa tgaggcaaac gatttaggga taatgaccaa
    13021 taccatagct gcgatcatca agtaagcaag tgtcagcata gagtattcga ctacagcagt
    13081 cacgacaccg atttgtaatg ctccttctgc tacaagattt ccgccaaacc acaaaataga
    13141 agcataaacg attcccatga gcaaaaaagc aattggagtg acccaagcaa atattcggtt
    13201 cactttgatc atttgattag catagtcagc aaagctctta ttcgttcgct tttcttcata
    13261 tgattgatta tcaaaggcac ggatcatatt aatcccagta aagaattctc tcaatgtgat
    13321 ggtgatccga tccatttttg gctggatact caaagataaa ggtgcccctc gtttcatcag
    13381 tcgataaaca atccatacat aaaaaacaac agagatcaat gtaacccatg ttagtaatag
    13441 agaataacga aacatcatga gaatggtgaa aacgcaaata accggtgctg gtaagatcat
    13501 ttggcaaaat aaaacaatca tttgctggac attatcgaca tcattcgtca ttcttgtcaa
    13561 aagagatcca gaactaaaac gactgatatc cttcattgac agctgttgga atttacggaa
    13621 atatttctcc cgcgtttgga atccaaacct agcggaagcc aatgctgaaa agtaactaga
    13681 ggcaactgca gcgattgttc ctagaaacgc tacaagcagc atttcaactc caattttttt
    13741 tataactgag gcttcgccac ttgctatacc tgtgtcgatc agctttgcta ctaattttgg
    13801 tacacctaat gtaccggcga tttctaaaca gatcatcaca aaggtaagga gagcgagtcc
    13861 tttgtttttc tgtaagaatg tttttaagat agacatatta ttcctccttt tttcctgata
    13921 cttttcgatt ctataccttc aagtaacttg aaggtcaata gaaaaaagaa taaactgaaa
    13981 aagaggtgag aaaaaatgaa aaaaatgtat acgattggcc aagtatcgga actatttgat
    14041 ttgccaaaat caacgatccg ctattgggat gaacaaggat taatccgttc aagtagacaa
    14101 gaagagaatg attatcgatt gtttgatatc gatgatattt ttatgcttta tgatatcgtt
    14161 ttttatagaa aattagatat accgatcaaa cagatgaaga atttatatgg aaaaacattg
    14221 acggagttat atgagacact tgatgaaaca gaaagaagaa tccataaaga actggtggtg
    14281 atgaaacaaa aacaaaaaga gataagggag caaaaaaaac agttgaaatt gatgatcgat
    14341 acaaacgagg aagaatttcc tgtagaagag atcccttttg actgtatgat ctcaacggag
    14401 tttgaagata ttgtggaaat caaaaaattt ctacctaact attcaagctt tgggatgatg
    14461 agtatgccag cttccagttc acagacgatg tatggatttt ttatcgaccc tagtgaagta
    14521 catttattta ctcaagacgt gatttgggag aaaaaagata cggctgttta tcgtcgattc
    14581 ttattgaaat ccgagatgaa ccatgcggaa cgtaataata ttttagaaat cagagaaaga
    14641 atgtatgaaa aaggatggaa aacaggagaa gtgatcggcc agtatctatt gacgaatacg
    14701 gatgaaaaca atatcagaac tgaatattat catgcatgga tagaaatgaa aaaataggaa
    14761 atatgtgttt aagataactc gaaaatgatg gttgttttag ttataaagaa aaattttctg
    14821 gttcgattac gggtttaatg tgggggatta ttagtcgtgg atagatagaa ttttagtttt
    14881 ttagaggaaa tatgtcttta aaaatggaat ggttgcaatt cttagcgata tatacgatgt
    14941 aattgtcctc ttaaaaaagt atggaaaaat aattgtattg ggtgttatgt ataattcatg
    15001 gtgaactgag actaatgaga agaaaaattc atattgttat gagtgagatt gtatcagatg
    15061 agaatgataa attaaatagg tgcataaaag cattacactt aaaatacatt gttgcttaca
    15121 ttattacaat atgttaataa ggcaaaaata aatagatgga ttgaataaac atatttttta
    15181 atcaaatagt ccctactgtg actttcctca gctatcggtg atactaccga tagcattttt
    15241 ttgcttataa aataattata agtatttaga gatttttctt ctaacattct tcaaacaaga
    15301 aaaatcacaa aggttattgt tatttttata tattcttttt aagaatgatt gtttttttat
    15361 agaaatttta aaaaataaaa cacgttattg atggatagtt agtttgttat ttggtaatat
    15421 tatagaagtt gactggtagt gatttatttt tcgtcgtttt ataaaaatgt tattttttgg
    15481 aaaaattcac aaatatgtaa cgaatgtttt tctggaattt gctgtgttaa aaagcagtat
    15541 gactaatata aatactattg tcatttatgc aattttttat ggatagtaat tttagagggg
    15601 aatcaatgaa ggaagaatag aagggaaaaa tagttgcaaa tacgccagtt agcaaaaata
    15661 gcaatacatc ttcaagcttt aaaattcgag agaatcatct tggtagtgga atcaactaca
    15721 acaaaaggat taaaccaatt aatagataga attaccaatg gaaaaataga aaaggtagtt
    15781 attctataca aaagttgttt aattcgcttc ggttatgaac ttagtgaaac cctttgcaat
    15841 atatggtaca gaatatagaa attattgaca ttacagaaaa tgatgaagat ttaatacaga
    15901 ttgtcactat ttgtagatac cttttacaag gaaaccgagc caataaagct aagaagatga
    15961 ttaaggagtt gatggaagac gatacaagtt aagaaagtga aactacgccc aaataaagag
    16021 caaaaagagt aattggacgt ggaaaatgca agaaatgaac catcgattcg gtggacagtt
    16081 tattagtaat gacggtttga gaacatccat gacgagaatg aaggaacatc ctaaatgcgt
    16141 ttagttgaac caagtgccaa ataatatggc caaataagcg atcagttatc gatgaatccc
    16201 aagtattcta aactatatat tcattttgat gctaagcatt gatatatttt tttgggattg
    16261 atgaaggtta accaatacag ggactcactg atgaaattct agagtctgac cttggtatca
    16321 acgatttagt gattgtttct aatggaaaag tataaaagta tataagtaca ttaacaaaat
    16381 aactattagg gttacgctcc agcttgtgca aggcactggc aggaggaatc gtgaaatcat
    16441 aattatggtt aagccacacg gacaactaaa agtcatacaa agctatcgaa tcaatcaaca
    16501 gaagacaatc ggcaatcatt agctttgttt acagcttaca ttgcaacgct tgagttgaac
    16561 cgaaaatacg agggaggtaa cgcagccatt ttgagttggg gaatcttttt agaaaattat
    16621 cgggtacctt gttgtttagt caaaattata agggggagta atagtgagga acaaatttca
    16681 taaactgtta agtatgtttc tagttatgct tattgtgatc aatcaatttg ggaacataac
    16741 ggttctagct gagtctttag cagaacagcc agagcgttcg ctgacgatcg aagttgatca
    16801 agaggaggct gctttgggtg agcagttcca gttgcgagtc agaggaacac cagacctgct
    16861 aaatgcattg gtcattgata agagtgagga tttgattgaa ggggaagtga agcaactcaa
    16921 tgattacgaa cgtctgatta cttattcttc ccagcattca ggtaaatacg aattgaccgc
    16981 aagaagcggt gatattcaat caaatacggt ctcaatagaa atcagtgtac cagaagaaag
    17041 cacaagtgag tcttttaatg taccagaaga aagcacaagt gaatctttta atataccaga
    17101 agaaagctta agtgaatctt ttaatgaagc cagatcatct gagccatcta taacgcagga
    17161 atctcagatt gaaagacaag aggagctctt gaattatgac atcggcatct ctgctgagcg
    17221 ccccactttg ccgagcaggc atacacaaac gttaaaggga tacattcaag gaggcgctgg
    17281 cgatattctt gaagaatatg gggagatggt cgtacgagtt cctagagcat cgattggctt
    17341 aattgagttc attagagtgg aggtcgctga tgattcacct ttaaagccac tggcatttta
    17401 ccaagaaggc acagacgttg tttttccatt tacctttgat tatgaaggat taaatgaagc
    17461 cgctcagtcg tatacattca gtatcacgtt tgaagttgct ttctttcaat caggcggtac
    17521 tgagccaaat aatgactttc aagtgagtct tacttatgct gagcgagcag caaacacgga
    17581 attttcaatt acgcctattc cttcatcaag acccattttt tcgaaattat actatggctc
    17641 tagagaaatc gacagccaag gaaatcttgg gaatctgctg attgacgggc aagacgtgac
    17701 caacagtaat aaatttattt tgcaggtaaa ttatgaagga tcacgaaaag acggtgtagt
    17761 ggttaccgat gtactaccgc aaggaacaac tttggcaccg aatccgacta catggagttc
    17821 cactggaaat agagaaccta tttcaggtgg ggttcggatc gcagaagtaa cgaatccagg
    17881 tgaagcaaac gttagaactc attatgtttc cgaggaattt gtggacaaag tatcttttaa
    17941 taaggaaacg aatacacttg ttgtggactt tggtaatcta gcagagggtc aatcgtattt
    18001 tattgaatat gctcttgaag tgaccgatgt cgacttgttc aatgaaggaa gacaagagga
    18061 tgggttttct cggaatcgag cgagtatgac ctacaataat caggtaatga gccgagacta
    18121 ccgcttgaga atccctgaaa gtaatcttcc ggcaatctct ttaagaaaaa gtgtggacaa
    18181 aaatgttatc aatctcaatg aaaatgaact aacgtatact ctgcatgtct cgatgttttc
    18241 tggaacaaga aatacgcttg ttattgaaga tgttttggaa gaaggcatgg aatttgtgtc
    18301 atttctatca gatattccct ctttcattga tgccgaagtc aataatggaa tagttcgttt
    18361 cagtgcgaat aatacgattg gtggtccgac aagttttacc ctctctttta aggtcaatgt
    18421 agaggcgtat gcggtcggcg atcaaatttt aaactttgca acgcttgtga atagtgacaa
    18481 tcgattgaat acgagtactg taagaacaag gaaaatcgat gggcgtatta aagtaattaa
    18541 aactgatgag aatggccaaa ggctggcagg agcaaaattt gaaattttat caagcgattc
    18601 acaagtgata caagaaggta taacggcaga aaacggagaa tttttaagtg aaccgcttcc
    18661 tgtaggtagc tatcaagtcc gagaaacaga agcaccggaa gggtatcttt tagatgcgac
    18721 ggtacatgat gtttttataa atgaatactc catagaagaa gtgacgttaa ccattgaaaa
    18781 catccttgat acgggtggtg ttgaactaat taaacatgct gaaggcagtg gtgaagtcct
    18841 tcaaggagct gtttttgagt tacagaaccg agaaggagaa acgctacaaa ccggt
//
LOCUS       DACEXQ010000027        18568 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.027, whole genome shotgun sequence.
ACCESSION   DACEXQ010000027 DACEXQ010000000
VERSION     DACEXQ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.027"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..294)
                     /locus_tag="IXH04_000755"
     CDS             complement(<1..294)
                     /locus_tag="IXH04_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAQ1851385.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLL"
     gene            complement(296..550)
                     /gene="purS"
                     /locus_tag="IXH04_000756"
     CDS             complement(296..550)
                     /gene="purS"
                     /locus_tag="IXH04_000756"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAQ1851386.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            complement(550..1278)
                     /locus_tag="IXH04_000757"
     CDS             complement(550..1278)
                     /locus_tag="IXH04_000757"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAQ1851387.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1534..1911
                     /locus_tag="IXH04_000758"
     CDS             1534..1911
                     /locus_tag="IXH04_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851388.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            complement(2091..3386)
                     /gene="purB"
                     /locus_tag="IXH04_000759"
     CDS             complement(2091..3386)
                     /gene="purB"
                     /locus_tag="IXH04_000759"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAQ1851389.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(3399..4532)
                     /gene="purK"
                     /locus_tag="IXH04_000760"
     CDS             complement(3399..4532)
                     /gene="purK"
                     /locus_tag="IXH04_000760"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAQ1851390.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSIEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(4516..5004)
                     /gene="purE"
                     /locus_tag="IXH04_000761"
     CDS             complement(4516..5004)
                     /gene="purE"
                     /locus_tag="IXH04_000761"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAQ1851391.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(5420..6727)
                     /locus_tag="IXH04_000762"
     CDS             complement(5420..6727)
                     /locus_tag="IXH04_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAQ1851392.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(6730..7311)
                     /locus_tag="IXH04_000763"
     CDS             complement(6730..7311)
                     /locus_tag="IXH04_000763"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAQ1851393.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(7427..7524)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(7883..9175)
                     /locus_tag="IXH04_000764"
     CDS             complement(7883..9175)
                     /locus_tag="IXH04_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1851394.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(9172..9795)
                     /locus_tag="IXH04_000765"
     CDS             complement(9172..9795)
                     /locus_tag="IXH04_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851395.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(9798..10634)
                     /locus_tag="IXH04_000766"
     CDS             complement(9798..10634)
                     /locus_tag="IXH04_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1851396.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(10651..11538)
                     /locus_tag="IXH04_000767"
     CDS             complement(10651..11538)
                     /locus_tag="IXH04_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1851397.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(11554..12837)
                     /locus_tag="IXH04_000768"
     CDS             complement(11554..12837)
                     /locus_tag="IXH04_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1851398.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(13520..14203)
                     /locus_tag="IXH04_000769"
     CDS             complement(13520..14203)
                     /locus_tag="IXH04_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAQ1851399.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(14221..14838)
                     /locus_tag="IXH04_000770"
     CDS             complement(14221..14838)
                     /locus_tag="IXH04_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAQ1851400.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(14848..15672)
                     /locus_tag="IXH04_000771"
     CDS             complement(14848..15672)
                     /locus_tag="IXH04_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAQ1851401.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(15665..16225)
                     /locus_tag="IXH04_000772"
     CDS             complement(15665..16225)
                     /locus_tag="IXH04_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851402.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            16399..17355
                     /locus_tag="IXH04_000773"
     CDS             16399..17355
                     /locus_tag="IXH04_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1851403.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(17660..>18568)
                     /locus_tag="IXH04_000774"
     CDS             complement(17660..>18568)
                     /locus_tag="IXH04_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAQ1851404.1"
                     /translation="SMVTAGSLSLLYWPAAGIIALLSVGNTKRSTLRTGMNRIAAFIL
                     ATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVNSVLVTHYLVEENMSWQ
                     LIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFRNILRQMAEFLLSENKE
                     DVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMRRAQANVIKDMLENLER
                     IQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLYRQMPLPTSRPEFEDRA
                     ELFQFLQSFKSFIEIKAEFSQQKIQK"
BASE COUNT         5464 a         4087 c         3169 g         5848 t
ORIGIN      
        1 caaaagtcct gcttctgtca atatctggaa tccattgcat gtaccgaata ctggtttccc
       61 ttcttcagca aaacgaatca cttcttccat gattgtagaa aaccgagcaa tcgcaccgca
      121 tctcaaatag tcaccatagg agaatcctcc cggcagtaaa accccatcaa atccttctaa
      181 agaggactca tcatgccgta caaattcggc atcagcgccc atgatctcgc gaactgccca
      241 taacatatct gcgtcacaat ttgaacctgg aaaaacgatg accgcaaatt tcatgttata
      301 cttcctccat ttttttgatt tcatatcggt atgtttccat atttacattt gcaagcagct
      361 tgtcacaaat cgtttcaatc actgtatcga ttggttcttc tgcatcagca agttgtatct
      421 caaagtattt tcctattcgg atatcttcga ttgtttcata tcccatacga tggactgctt
      481 tttttactgc ctccccttga ggatctaaca ctgaatcttt ataggttaca tatactttta
      541 cgaaatacat catactcgct ccttttcttc agttagttcc gatagacggt tatatacttc
      601 ttgatagact gggatcaaat cacctagatc tctgcggtaa acgtctttgt ccatatgttc
      661 attcgtacgt tcatcccata atcggcaagt atccggtgtt acttcatccg caagcaaaat
      721 ttgtccattc gaccggccaa attctagctt gaaatctact aaaaccaaac cgaccttagc
      781 aaataattcg gtcaaaattt cattaatagc caacgcttgg gctttcagct cactgatttc
      841 ttccggcttt gcaagttgta agaaatagat atgctcttca ttgataaatg gatcatccaa
      901 ctggtcatcc ttatagtaga attcaatgat tggtttgatg aattctttac cttcgggaac
      961 acctagacgt tttgagaaac ttcctgctgc aatatttcgg ataaccactt ccagagggat
     1021 aatttgtact ttttttgcta attgttcggt tttagacaat tgttcaataa aatgattttt
     1081 tattcctctt tgagtcagat aacggaaaat tgcacttgtg atttgattat tcaattctcc
     1141 ttttccactt atctggtctt tcctcgcgcc attcaacgca gttgcttgat ctaaatattc
     1201 tatccaaatc aagttctcat cagaagtaag atatattttt ttggcttttc cttcatacaa
     1261 aagttctttc ttctccatgt acgtctctcc tttttattta cattaaaagc aaatacatcg
     1321 ttttttgttc gttttttcac tttaaatttt agcaatcatc tttttataaa tcaacaaaaa
     1381 tatgtacatt taaggcatta gattctttta atgttcgtgt tttatcagaa acgaaaatct
     1441 tataccatca acaagcgttt acatttatac ttaatgatgg tatattagaa ttaactaaaa
     1501 atttggataa tgggttcgag gaggcacttg ccgatggaag atgcaataaa acaaggtcag
     1561 ctttttttat cagaattaac agctgaagaa cgttcctgtc tatataatga tttgctgtcg
     1621 atcaaagacg tttatttatg caataaagat actagttttt atacattccg aaaaatccgc
     1681 cgagcaacca agcgctgtgc tgaatgccgt aaaaggactt ttctttatca gacagaacac
     1741 aaaggcgaac tttcaatcag tcagattcat gaacagaagc gtttcaaaaa tcattatcgt
     1801 atccagatct tattcaatgg acaagagatg gaatacgctt ttctcgaaaa cttgctgcaa
     1861 catctccata accagcattt tagttataat agccaagcca tttacgctta attcctctat
     1921 tttttgtatg taaagaggca cggtaaagag gtagccgctt ttaaaatcag gttcctatta
     1981 ttgataagaa aatataataa actaaaagaa gcagaaccgc taacggatct gcttcttttt
     2041 tggttatttt tgctattcta ttttaattga attttgcaac tcactcttct ttattccaaa
     2101 cctactcgtt tgaatatcac gtcaacgttt ttcaaatgat gatgataatc aaaggcatcg
     2161 tctatttctt ctttagataa taccgatgtg atggttggat cttcttctaa cagcgggcgg
     2221 aactgcactt ggttgtccca agcgtacgca gtttttggct gaacaagatc ataagcttct
     2281 tctcttgtca ttcccttatc gatcaatttc agcataacgc gctggctata aatcaaacca
     2341 aatgttgcag acatattccg cttcatgttt tcaggaaaga ccgttagatt tttaacgata
     2401 ttgccaaaac gatttaacat gtaattcaat aaaatcgtgg tatcaggcaa gatgattcgt
     2461 tctgctgaag aatgagaaat atctctttca tgccataatg tgacattttc ataggcagtg
     2521 accatgtgtc cgcgcaccac tctggctaga ccagccatat tttctgaacc gatcggattc
     2581 cttttgtgcg gcatagcaga agaacctttt tgacctttcg caaaaaattc ttctacttca
     2641 cgtgtttcgg atttttgcaa tccgcgtatc tctgttgcaa aacgttcaat acttgtagca
     2701 ataagcgcca tcgctgagaa atattcagca tgtagatccc gagggagaac ttgagtagaa
     2761 atttcttgtg gacggatccc caattgttta catacatact cttcaataaa tggcgggata
     2821 tttgcgaacg ttcccactgc tcctgagatt tttcctgctt ctactccttt tgccgcatgt
     2881 tcaaatcgtt cgatgttccg tttcatttct gaataccatg tggcaagttt caatccaaaa
     2941 gtagttggtt cagcatggac accgtgtgtc cgacccatca tcactgtata tttgtgttct
     3001 tttgcttttt cgccaatgat ttccgtaaaa cgtttcaagt cttcccgtaa aatatcattt
     3061 gcttgcttga tgagatagcc ataagcagta tctacgacat ccgtacttgt tagaccataa
     3121 tggacccatt ttctttcttc tcccaatgtt tcagaaaccg ctcgtgtaaa cgcaacgaca
     3181 tcatggcgtg ttaatttttc gatttccaag atccgatcga tatcaaatga tgcatgctca
     3241 cgaatttttt tcacatcttc ttgcgggatt tcaccaagtt ctgcccaagc ttcatctgct
     3301 aaaatctcga cttcgagcca cgcacggtat ttgttctctt ccgtccagat attccccatt
     3361 tcagggcgtg tataacgttc taacatgttt tttctcctct aatcccaaat attcgtttgt
     3421 ttgatttctt ccagcgtttg ctggatgtct tctgtcaaga tagtgatatg tcccatcttg
     3481 cgaccttttt ttgcttgttt ttttccatag aaatggaatt gccagtctgg cttattctca
     3541 atcaagcgat agctttccag cagttgttct cccaaaacat tgaccattac tgcattgctt
     3601 atcaaacgta ctttttctcc taatggccaa ccacaaatac ctcgaatatg ggcatcgaac
     3661 tgactcatag aacaagcttc aatagaataa tgtccagaat tgtgaggacg aggagctaat
     3721 tcattcacat atagcccgcc tgtttttgtt aaaaacattt ctacccctaa aacacctgaa
     3781 aggctcactg cttctgcaat cacacgggca atacgctcag cctcctcgat cacatcctga
     3841 tcaatcgcgg ctggagcaat cgtttcatga agaatattgt tgtggtggat gttttccacg
     3901 accggaaaag tcgtgaaatc tccttgacca tttcctgcca ccatgatcga aatctctttt
     3961 tcaaacggga tccatgcttc taaaacacag gttccttctc ttaaaaggtc catggcagga
     4021 gctagatccg cccgactttt caaaacatac tgccccttgc catcatagcc gcctcgagtg
     4081 gttttcaaaa cacaaggata gccgatcccg tcaatcgcat cttggatatc cgtcggactg
     4141 acgatcgttg cataaggagc aatcacgatg ttattcgttt ccaaaaaaga tttttctaac
     4201 aggcggtctt gcgtaatcgc aagcaagtct gtcccttgag gaataaatga catgggtaaa
     4261 atcgcattta acgcctctat actgacgttt tcgaattcat aggtaatgac atctgttctt
     4321 ctggcaagct ctttcagcgc aaaggtgtca tcatagacac tttcaatgtg ccaatctgca
     4381 acttgtgcgg ctggacaatc gctcacagga tccagcacac ctactttaaa gcccatttct
     4441 tttgcactaa tcgccatcat acgacctaat tgaccgccac cgacgatccc gatcgttgaa
     4501 ccaggcaaca aaggtttaac caagttgatc actactttct ataacagttt tagccaacat
     4561 tgaacgtctt tcagccaatt tagcctccaa ttctaaatca tacatggaaa gcatctgtat
     4621 cgcaagtagt ccagcattaa ttgctcccgc ttttccgatt gctgtagtag ctacaggtac
     4681 tcctccaggc atttggacaa tcgaaagaag cgaatccaaa ccatttaatg ttcgtgattg
     4741 gaccggtaca ccgataaccg gcaaagttgt ttttgatgcg accatacccg gcagatgcgc
     4801 ggctccccct gctcctgcaa taatgatttt gattcctttt tttcgagcgt tttgtgcata
     4861 ttcaaacatc aaatctgggg ttcgatgagc agaaacgacc tttttttcat aagacacacc
     4921 gaattcatcc aatatttgac atgcttcttt cattgtttcc caatctgaag tactccccat
     4981 aatcacagag atttttgctg tcattgtttt tcctcccagt ttttctacat tcatatttta
     5041 acaatcactt tttttgatgt caaagaaatt tcgaatgtta acaaagaaaa acattttatt
     5101 gttcgtgttt attaaagata aaaaaaagga atgtgacaaa agttaggtca cattcctttt
     5161 tatggataaa cggtgttctc ctgccaactc ctcggtcaca aggaacaata ttcaacaaac
     5221 atgagaagct tttttttgaa attgttcctt gtgactcgga gatgaacggc aggctcacga
     5281 cctcttcctt caacggtgtt caatcagctg accctcggta ttagctcaaa caaacggcaa
     5341 aatacgagga actatttttc cgtttgtttc tctaacaatc aggtcaaaac gctgatttca
     5401 caacctttct tttgctctat tacttcttga aggcttcttc caatatttct tcatcttttt
     5461 tcaatccatt aaagatcaaa ttcaatatga tagccgtaat gctactcatt acgatgccat
     5521 ttccagtgaa catttgcaat gtttgcggca tttgactgaa taatgtcggc gtaacattga
     5581 aacccagccc aaatccaaca gaaacagcaa caatcaatag atttttatca ttgctgaaat
     5641 caacttccaa cagcatacgg attccttgga ctgccaccat cccgaacatg actaacattc
     5701 ctccaccaag taccggctca gggatgattt gtgctaacgc accgatttta gggaataatc
     5761 ctaaaacaat caagaagaat gcagagaaat agatgggttt tcttgttttg attcccgaaa
     5821 gttgaaccaa tccgacattt tgagaaaagc cagtataagg gaatgtattg aaaataccgc
     5881 ctaagataac ggcaagtcct tctgcacgat atcccttttt caattcttct tctccaacat
     5941 ttttcccagt aatgtcacca agtgcaaaat atacacctgt tgattctacc atgctgacca
     6001 tggaaatgat gatcattagt aaaatcgacc atacgtcgaa agttggttta ccaaaataaa
     6061 agggttgtgg aacatggaac aacggcgcat ctccaatcgc gctcagatca atcattccca
     6121 tgaaagcagc taagatgctt cctccgacta aaccaatcaa taccgcgatt gaacgaatga
     6181 acccttttgc cagcatttgc acgcctacaa ttaggagaat cgtcacaaat gcaagtagca
     6241 gatttgtttt gtcaccaaaa tcagctgctg ttgcattacc tccacccatt ttctcgattg
     6301 ctacaggaat caaagtcaaa ccaataacgg tgataaccgt tcctgttact ataggaggga
     6361 atagtttttt aattttagaa aagacaccag aaactaacac aacaaagatc ccagaagcga
     6421 taatagatcc gtagatagct cctactcctt cattggttcc gatcaagatc agtggagcta
     6481 ctgcttggat cgcacatcct aaaacgacag gcaagccaat tccgaaaaat cggttaactg
     6541 tcaattgcaa taatgtcgct aatccacaca taaagatatc aattgaaatc aaataagtca
     6601 tctgttcttg attaaagcct agtcctgtac cgatcaaaag aggcaccgct actgctcctg
     6661 catacatcgc tagcagatgc tgcagcccta aaaccgcagc ttttccattt tgtgtttctt
     6721 tttccacgat tatgcgtcct cctcaagaaa ctcaacttgt ccattcgtca aggaagcaat
     6781 acgcgcaaga gaaacgactc tcaatcccat ttcttcaagt aactgacgtc catcttggaa
     6841 agacttttcg atgacgatcc cgattccttc tacttgcgct ccagcttgct ggcataattc
     6901 gatcaatcct tttgccgctt gtccgtttgc caagaaatcg tcgataatca atactttgtc
     6961 ttctgaagat aaaaatttac gagaaataga aatcgtactt gtcacttgtt tcgtaaaaga
     7021 ataaacagaa gctgtcaata attcttcatc catcgtcaaa ctttttgctt ttctagcgaa
     7081 gatcattgga acatctaatt ctcttgcagc aaataaagct ggtgcgatcc cagaagcttc
     7141 aatggtcaca actttcgtga ttccttgttc tttaaatact tctgcaaacc gttttcccat
     7201 ttgttccatc aatacagggt ctacttggtg agtgacgaag ctgtcgactt ttaatacgcc
     7261 ctcgcctaat atacgtccgt ctttttgaat acgttcaact aattctttca ctttatttgc
     7321 tcctttatca tttattaata ccgaaataac cgcttttgtg caaaaaccta ctatgtttct
     7381 gttaaagaaa aacaccctcc actttatcat aagaaaagaa gggtgaaata gccaatcttt
     7441 tacttatagt ccagcattta cggtactggc tagaaactgt catccatatt atgacgatat
     7501 ataagtaggt atgtaaaact aataatagtg taaattcatt ttcttgcaac tgttttgcac
     7561 gtgaaggcag tttgccatat ttgaataaaa aatgttgttt caaatattga aaacagtcta
     7621 acactacttt ctgaaactga caacgtggtt tacttatttt tttgtaaaat ctaatgattt
     7681 ttaatgcatt attccttttt gttcgggttt aaatagttat ttttattttc tcctatctaa
     7741 aaaagcagcc cacataaaaa gaaggcatga caaaaagcat gtcatagccc ctttttccga
     7801 ataaatagtg ttcaaaagac agcctatcgg caataaggca agattttata aactacggga
     7861 agtaatttcc gtaaatctat tcttattctt ttaagctgac aagctcgtca atcactactg
     7921 cttttcctga agcaatagaa tgatttgctg cgattcctgt caaaatcgac aaaataccat
     7981 ctacgtgagt tgctgcccga ttccacggat cttcttcttt ttcttcacca aatagatcat
     8041 ctaataaacg cggatctcct ccgccatgtc cgcccgcttc ttgttcgacg attacctcat
     8101 atggctcacc gaacatcgga tagacatgga tggactgaag aacggctgct ccttcatcct
     8161 ttttatctcc acctgcattg atgtaagact tttcgatcac tttcatttcg atccgtcctt
     8221 tgctgccatt gaaagaaaca ttgaatcctt cccacggcat atacgcgttt agcgaatacg
     8281 tcaaaatcgc accagtttca tatttgaccg ttacgcctag tgtatcttca atagagatat
     8341 catcggaaaa tacactgcgg tcacgaatat atccactgtc tttttctgct tctaaataca
     8401 tttctcgcaa ttcctcattg gctgacaggt ctatagcaaa tggatcagct ttagctagtt
     8461 cgcttccata gcatcgactg taaaatattt ctactccgcg tttctccgca ttatgcatcc
     8521 cataaaagtt taatccaccg aatgcaaata ccttttgggg acggctgttc aaccaaaaat
     8581 tcactagatc aaaatgatgg gtagatttat gaacaagaag cccgccgcta ttgtgcttat
     8641 ttcgatgcca tcttctgaaa taatctgccc cgtgttgcgt atttaacagc cattcgaagt
     8701 ggatagaaaa cacatcacca atcacatcac tttctatcag ttcctttatc ttcgtgtgat
     8761 acggtgcata acgataatta aaagtgactc gcaggcttct tcccgtctgt ttttggaccg
     8821 ttattatttc ttgagctttt tcctcatcga tagtcagagg tttttcgcaa ataacatcat
     8881 atcctttttt cattgcacga atgatataat catgatgcgt tcgatctact gacgtaataa
     8941 tgacttcagc cggccgtacg tcatccagta gttgctcaaa ttgtgatggc atacatgtct
     9001 ttactttttt ctgtccatac ttgttcacaa gtatccgatt tgcataatcc atccttgttt
     9061 gagatacatc acaaaaaccg actagttcac aagtttgatg atacgtagag gcaattgcct
     9121 cataaaaaaa acgcgctctc ccaccaagtc cgacttgtac atattttttc ttcatttaaa
     9181 gtcattctcc ttttctttta agcaaaatac tgtgacaaat ggcagccctg gatacagtaa
     9241 tgcagtgagt aaaaaatcat gctgtataca ccagaacagt ccatacaaaa ggcataaata
     9301 ccacagaaac cccttgacgt tttttgacaa cagccagata ttccttttga tcaaataaac
     9361 aagactatct gctttatctt ctttgcagat atcaattagc gtactaaaaa aactatggga
     9421 tacaaaaagc aacagacaca atagaaaagg aatcagatac caagctccaa tctttcccac
     9481 taataaatac caatcaactg caaaaataat gacaaacagc tgaaacaacg taatgatgaa
     9541 acacatccgt ttgatttttc ctttcctcca acataccttt ttttctgcca agtaatggac
     9601 agatagataa taagaaggat acatcatcaa aaaataaatc agatagacaa cacgagcaga
     9661 cagcactaat cctaaagtga acaaacatcc tagaactaaa gcgaaaagag cattactgat
     9721 aagactggtt aaaaaaatcc gacagacata gtagttgagc ttgtaaagaa acatctcttc
     9781 tattggcttt ttcatattca gcccttcaga ccgcttgtac tgatcccctg gacaagatat
     9841 ttattgaaga ataagaaaat cagaaaaacc ggtagcagcg acaacgtaga catcgcaaac
     9901 atactcgcgt aagtcgtact agatccagcg tctgcaaata atttgatggc aatcgatacc
     9961 gtgtaagatc tcggcttgtt taaataaatc aatggtccca tgaagtcgtc ccatttccaa
    10021 taaaactgga tgatgatcgt cgtgatgatc gaaggtttca gcaaaggaaa aataattttg
    10081 gtaaagacac tatacttgct gcatccgtca ataatcgctg attcatctaa ctctttgggg
    10141 atgctgacca tgaattgcat gatctggtaa atgaaaaatg cttgtccaaa aaaatgtggt
    10201 aaaatcagcg gcacatacgt cccaaccaaa ttcagccgat tgtaaataat aaactgcgga
    10261 atcaaaatca cttgtgcagg aatcatcatc gttactaaca tgacgacgaa ccagaatctt
    10321 tttcctttga aatcgattct cgataatgcg taagcaatac aagtcgagct cagaactgtc
    10381 cctatcgtcg aaactgcaga gatgacaaac gaattcctaa agaaagtaaa aaatgaaatt
    10441 ccagcaaacc ccgcccatcc ttttgcgtaa ttttcccatt gaaaagtctc tggaatcaaa
    10501 ttcagcgaac cactcataat ctcttgatcc gttttaaaag aacctgcgat catccataag
    10561 accggataaa tcatgactag acctaatagc aaaataaaac cgtattgaaa ccatttcgtt
    10621 actgtattat tcatttcttc ctcctctcat tcacacaacg acccattttt ttgatgattt
    10681 gaaaatcaga aaggtgacag ctcccataat caacagcatg atccacgaaa tagcactagc
    10741 ataccccata gaattaaaag aaaaagcttg gttatacaca taaagagcat aaaagttcgt
    10801 ggcatcattc ggaccacctt gggtaatgat aaatgcctgc gtaaaagtca taaacgccgc
    10861 gattgtctgc ataaccaagt tatacaaaat gatcggcgaa agaagaggta acgtgatctt
    10921 gtaaaacgtc tgccatttgt tcgctccatc gatctctgcc gcttcataat acgagcctgg
    10981 gatctctttg atacctgcag caaagatgat cattgccgaa ccgaattgcc aagcagacat
    11041 caaaacaaga gggatcatga ccagtttttg atcgctaaac caggaaagcg caggtgcgcc
    11101 taatcgttcc aggaaaatat tcactaaccc gtttttagaa aacaactccc gccaaactag
    11161 agcgatagaa atactgccac cgatcaaact aggcacataa tacaaagaac gaaagaaagt
    11221 cgttcctttg atattcctcg ttaaaagata agcaatcaat agaccaaaga tcagtttcac
    11281 aggtacagat gcaaatacat agagaaaagt gactcggatg gagttcaaaa aacgggcatc
    11341 actcagcatc tttatataat tatccaagcc gatccatgag actggatttc ccaaacgata
    11401 atcggtgaac gaataataaa gtgacaagat catcggtatc actgttatca atagaaatcc
    11461 taggatgaat ggagaagcaa atacgtaacc gaccacattg tcttgatcca agaaccgttt
    11521 tagtttttct tttttcatgc cttcctcctg ctgctatcct agaatgtttg acgcaaactc
    11581 ataaagactc tctgctgctt cttctgctgt tacttcttca taaatgactt tttcaactaa
    11641 aagattgtat tggtcttgaa tttccggatt caaagcacta tcaaagacat tcacttcgcc
    11701 gttgtccata tcggaaatca tttctaaata atcaaatgtc gcttgcaaag ttgcatcatc
    11761 tgcacgagcg agctgttctc gaatattcga catgattggt acaccacgtt ctccttttaa
    11821 aatttcattg gcttcttcac tgttttgaaa gaaatcaata aacttagcag cttcttcttt
    11881 atgggctgag ctttttgcca tactcagcat ttgcgaagaa cgtaaaggga taccagattc
    11941 tccatctgct tttctgcgag gtggattaat caactgaatc tccatatctt caggagcccc
    12001 tgacaatatt tctgtcaact ggttacttga tagaaacacc attgccgctt ctctatccct
    12061 aaccgcctga aaagacagat ccgtcgtact tgcagctcca ccacctggtt ctggatgggc
    12121 ccctttgtct accagatttt tacgcagctg gaaaaaatct gttagatagt ctggattttc
    12181 aaatcccaag ctgttagata gatcttcccg attataaaaa ttcaatcctt tctcaacttg
    12241 tggtatttga ataatacagc cagctataaa atcttcgaat cttgaaatag catagattcc
    12301 taattcttca gtgatttgat agctgatttg ttcaaattca tcccatgtcc aatttgaagc
    12361 tggttcaggg atatttaatt gttcaaatat atttttattg taagcaatcg caaacgaatt
    12421 caccccgtta gacagtccaa ctaattgccc gtcgtaagtc gtcgttgcta aataggattc
    12481 atctgcatta gaaatatcaa ttatcccttg ctccacatat tcatctaatg gttcaatctg
    12541 attgatgtat tgaggaaatt gattgttcag ttggaaaatg tcccagatat catctgatgc
    12601 tgctagtgta cctaattttg tgtaatatcc ttcataatca taaaattcat aatcgatcgt
    12661 cacatgggga tttttttctt cataaagctc gattactttt actgttgcat cgtgtcggac
    12721 ttgcgaaccc caccaagcca tgttcagcgt gatttttttg tcatcttctt gcccacaagc
    12781 cgtcaagaag aataacataa cacttaaagc taagatgatt ctcattcttt tcttcattct
    12841 ctgcacctct acttccattg ttatttttca atatactgta tttttataca gaacatattt
    12901 attaaacgat ctttttcaag gggaataaaa accaagactt gccttatcgt tctgcctagg
    12961 atgaacaact caatactttc aagaaaaatt agatagtgct gattcttctt tcatcgctaa
    13021 aaacacttct tgcttcaatg atgggaggat aattacgcaa agaaaaaagg atatcagaca
    13081 actgcccaat atccttgaca tagctaaata agcatgcttc ttcgtgtgcg ctcttcccta
    13141 aaaaacagaa aaataactag taattctgta tgaaattagg tacaatatac acgtcgatgc
    13201 acaagcattg actatggcaa cttgattggt cagttcccgc tgacttttca agccccgaca
    13261 atttagctgc tactaaattg tcgggtgcct tttttagttt ttattcgttt ttcattctat
    13321 agaaagcgct tcaataaatc aagacatttt tttgttagaa tgctaataat aacaaaaggt
    13381 tttaactctt cttatgtaca ttacaggtaa aaaaacaaaa aaatttctct tttttttgaa
    13441 ttctgaacaa atagtactgg agtacaaaag acgaggaaga gccactatct gctcttcctc
    13501 gtcttttgat tctcacctgt tattcatgat tctttttctc agtttcccga tatttttcca
    13561 ttttacgact aaaaagctct ccgctacttg gtggcgtata tgtgatggca tttgctcctg
    13621 cttgaatggt ctcagcaata ctttcttccg ttggaccacc tgtcgcaatg ataggaagtt
    13681 caggatattt tttacgaaaa tggcgtactg cttctgcagt ttctttacct gcactgatat
    13741 tgataatcga aataccagct gctaactttt catctatttc tgtatatttc gacgttaccg
    13801 tactgataac tggaatatca actactcgac aaacttcttt tactgtctct aaaggcgtag
    13861 gaccgttcaa aacgacccct aacgaacctt gcgcttctgc gaacatgctc atgtaggaag
    13921 aacgagcacc ttgtgtcaaa ccaccaccta caccggagaa aactggaatg tcagctgcct
    13981 caataatgct tttagtaatc gctggatgag gtgtaaaagg atacacagca attactgcat
    14041 tcgcatttgt attacgtatg atcgcaatat ccgtagtaaa gatgatcgaa cggatcaatt
    14101 ttccaaatat cttgatcccg cttgcttcta aaatgatttc cggtactctt acgatatctt
    14161 ttctcaattc cgtcatgatt tctggcgtaa attgttctgc catcagccac ttctcctttg
    14221 ctatctccgg tcatactgat tcaattgata cgtctcgatg acctggatga ttttttgcgc
    14281 ataaccgggg tctgtcgcat aacctgcatc ttgcaatgct tgtgcggctt cctgatagtt
    14341 ggtggccgta atcacttttt catacagcgc aggattccaa tctactcctt ggacgaacag
    14401 ttgtgtatgg tcatccatgg actgttccca tgaatcatac actttgaagt ctccttggat
    14461 agttatccat tcttcgttaa caaattcctt tgtttccagg cttactttct tctgatcccc
    14521 ataagccttg atcccgaaaa gattattgta tttgctagcc aaggtacttt ttccccaatt
    14581 tgattctaat atagcttgtc ctaaaatgat actaggaagc actccataac catcttgtag
    14641 ttcttgggca tgcggaacaa gccgttcaat aaattgctga tgggattgcg cttcattttc
    14701 ttgctgatta tcagtaaaag gagaagacag attcttcaag gaaaaaacaa aggctaagag
    14761 tatcactgct acaccggcaa ccactgctgg ccaacggata ttcttgcgct tttgcttttt
    14821 ccactttttt ttcgccatga tcaattccta cttttcattt aattcattca gatattcttc
    14881 tagcgtcaat tggtgtttgg tgatatactc agcgttttct ttaccgacat aacgtaagtg
    14941 ccaaggttca tatgtgatct tagttatatc ttcgtgtcct tttggataac gaacgataaa
    15001 tccatactgc gcagcattat ctgcgatcca tttagctcca ggctgatctc cgtaacttgc
    15061 atccaataca gtagaaggat aactgttgta ccagttttga tcgacgacat cgactgccag
    15121 acctgtgtgg tgttcgctgt atcctggctc cgtaatcgtt tctttagtaa tctttgtcgc
    15181 ctcctcttct gatacacctt gacgactcat cacatcttgg acattttgtt cgaaaacttg
    15241 ttgttgggat gatacagacc ggtaagctga gaccatcact aatggaaatc cagctgcttc
    15301 agcagccttg gcaaatgctt catattctgg agcaatgcgt ttgtcgatca agtatccgtt
    15361 acttaatgat gccaattgag tagactcatc aatttctttt tctaatttgt gatctggacc
    15421 aaccaaaact agcttccaat cgtctgtttt aacatctggc aattgtttgg acgtacgatc
    15481 ttttgtttct ttgtccttta ccttgctgct ggttgtttga ctgttctttt ctttactagc
    15541 agttgttttg gtgcttgttc gttcgactgc ttgattgccg gcttgaacat gcgaatcatc
    15601 atcgctaaaa ataacatagc ccattgctgc gatcataatc acaactgctc caaatcctaa
    15661 aaatttattc acttgaaaaa tcctttcgtt gttcttcacg ttccccattc gcctcatgaa
    15721 cgtaagtttc ttccagattt tcattcaccc aatcgagaag ttcgattttt tctccttcga
    15781 tctgtttttg aacatctgct ggcagacgaa aattcaaaat atcatcatag ccccattcat
    15841 catattgaat acttactttc aagtcaaggt atctaccagc ctgctgatta tttttcaaag
    15901 ggatcagctc gacttttgct gctttgctgc taagccattt ttgcgcgatg cgtgttttga
    15961 tttgcttgta ctctgcaacg gcaacgtttc gtttttcggg aaaaataatc gtttgacgca
    16021 aaatcttttg cgttagcatc cattcataat cagtgaaaag attttttact ttctgcatcg
    16081 cttcagatgg cagatttttc tcgccatctt tccatttttg ccattgttct ggactgatca
    16141 gcaagtattc atctaaaaaa tttttttcag tttcaaactg ttctaataat acactcataa
    16201 taagatcaat atatacttga tgcacgttta tcccctcctt ttaactattc tacttaagtt
    16261 ttgatcaaaa agcacctctt aaatcgctgc tttcattttt tttaatgttt caagtcctct
    16321 cctctttatt cttcgtgatt tttgttataa taatagacag acaagaaact caatccaact
    16381 atgaaggagc gttttcagat gaaaaaatat caatggggaa tcgttggtct tggtaccatc
    16441 gcacatgagt ttgccgaaag cttcaatcaa gagacgagcg aactaacagc agttgcttcc
    16501 agaacttcag aaaaagcgga aaacttcgct caccgttaca atattccaaa agcttacgga
    16561 agctatcaag aaatgttaga cgatgctgaa atcgacatcg tgtacattgc tgtaccaaat
    16621 agacagcata tcgaccatat cttggcagca ttggaagcag gcaaacacgt tctatgtgaa
    16681 aaagccatca cgatgaataa aaaagaactg gctgacgcta tgcgcttagc agaagaaaag
    16741 aatttgatct tagcagaagc gatgacaatc ttcaacatgc cactttatca gcagttacgc
    16801 tcaataatgg atacaggaaa actgggggct ttaaaaatga tccaagcacc tttcggcagt
    16861 tacaaagaac cagaccctaa aaaccggttc ttcaatccag aattagctgg aggagctctg
    16921 ttggatatcg gtacgtatgc tgtatctttc gctcgtttct ttttgagctc tcaacctgaa
    16981 gtagttgcct ctacaatggt tccttttgaa acaggcgtgg acgaacaatc cgtcactatc
    17041 ttgcgtaaca aagaaaatga acttgctgct gtcagcttga cttttcaggc aaaaatgcca
    17101 aaagtcggta tcgttgcctt tgagaacggt tatgtgacga ttgctgatta cccgcgtgct
    17161 gatcgtgcgg agatcttatt tacagatggt acaaaggaat tcatcgaaag cgggaataca
    17221 tctgaagcaa tgaattacga aataaagaat atggtacaca tgattgaagg tgacttgccc
    17281 aatcgctctc tcttcttgac aaaagatgtc atcgaaatct tggatcaaat gcaagttcta
    17341 tggaaaaata tgtaatttta aaaggttgtg aaaaagctgt tctgcatcaa gtaataagaa
    17401 cagccaaaca aaaatagctc ctcctatttt tcgtttggct ggacttatta ccgcagagga
    17461 aagcttttga acaccgttta agaaagaggt tgtgagccta ccgttcagct ccgagtcaca
    17521 aggagcaatt tcaaaaaaca gcttctcatg tttgttgaat attgtgactt gtgaccgagg
    17581 agttgacagg agaacaccgt ttaaagaaga ggttgtgaaa tcagcttttt gacctgaata
    17641 ttagctgaat tcacaacctc tatttttgga ttttttgttg ggagaattct gccttgattt
    17701 caataaaact tttaaaagac tgcaaaaact ggaacaattc tgctcgatcc tcgaattcag
    17761 ggcgactagt cggcagaggc atttgccgat acaatacata aacttcttca atacgtgtta
    17821 aaatttgtct gccatcattt gactctgaaa atgtttctgc agtataaatc aataaaccat
    17881 agatatgttt tccataataa gctggttgct ggattctctc tagattttcc agcatgtctt
    17941 taatcacatt ggcctgtgct cttctcatag agaagtaagt ttcgtagtat agcggctgtc
    18001 tgagccagta gttttcttga tgttctctgg catcttcttg gctttcgcga atgaatgtca
    18061 gtaaatgttc gcatttgatc tgcacatctt ctttattttc tgataaaaga aattctgcca
    18121 tttgtctcaa aatatttcga aattcttttt cgatctgtat ttggttttcc ttcagctgct
    18181 tcgtacgatc tggcatgtaa acatttgcaa gcaaagcaaa tactaatcca atcgccatta
    18241 atcccgcttc attgccgatc agctgccaag acatgttttc ctccactaaa taatgagtga
    18301 ccaaaaccga atttactact atcccttcgg ttaatttgaa gcgagaggca agcgggatga
    18361 acaggaataa aaaaagactg aagccaaaaa tagtataccc aacagcaaaa aaactgaaca
    18421 gtgctgttgc cgtagctaat ataaaggctg cgattcgatt catgcccgta cgcaatgtag
    18481 agcgcttggt atttcctaca cttaataatg caatgatccc tgcagctggc caatacaaca
    18541 gcgataagct acccgctgtg accatcga
//
LOCUS       DACEXQ010000028        18545 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.028, whole genome shotgun sequence.
ACCESSION   DACEXQ010000028 DACEXQ010000000
VERSION     DACEXQ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18545
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.028"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            288..656
                     /locus_tag="IXH04_000775"
     CDS             288..656
                     /locus_tag="IXH04_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ1851405.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            836..1777
                     /locus_tag="IXH04_000776"
     CDS             836..1777
                     /locus_tag="IXH04_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAQ1851406.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1846..2577
                     /locus_tag="IXH04_000777"
     CDS             1846..2577
                     /locus_tag="IXH04_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAQ1851407.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2721..4901
                     /locus_tag="IXH04_000778"
     CDS             2721..4901
                     /locus_tag="IXH04_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAQ1851408.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4921..5403
                     /locus_tag="IXH04_000779"
     CDS             4921..5403
                     /locus_tag="IXH04_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAQ1851409.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5419..6180
                     /locus_tag="IXH04_000780"
     CDS             5419..6180
                     /locus_tag="IXH04_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAQ1851410.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6311..7645
                     /locus_tag="IXH04_000781"
     CDS             6311..7645
                     /locus_tag="IXH04_000781"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAQ1851411.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7952..8437
                     /locus_tag="IXH04_000782"
     CDS             7952..8437
                     /locus_tag="IXH04_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAQ1851412.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8430..9113
                     /locus_tag="IXH04_000783"
     CDS             8430..9113
                     /locus_tag="IXH04_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAQ1851413.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            9239..11884
                     /gene="polA"
                     /locus_tag="IXH04_000784"
     CDS             9239..11884
                     /gene="polA"
                     /locus_tag="IXH04_000784"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAQ1851414.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11929..12765
                     /gene="mutM"
                     /locus_tag="IXH04_000785"
     CDS             11929..12765
                     /gene="mutM"
                     /locus_tag="IXH04_000785"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAQ1851415.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12729..13358
                     /locus_tag="IXH04_000786"
     CDS             12729..13358
                     /locus_tag="IXH04_000786"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAQ1851416.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13410..13742
                     /locus_tag="IXH04_000787"
     CDS             13410..13742
                     /locus_tag="IXH04_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ1851417.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            14054..14545
                     /gene="nrdR"
                     /locus_tag="IXH04_000788"
     CDS             14054..14545
                     /gene="nrdR"
                     /locus_tag="IXH04_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAQ1851418.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14568..15941
                     /locus_tag="IXH04_000789"
     CDS             14568..15941
                     /locus_tag="IXH04_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAQ1851419.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15941..16867
                     /gene="dnaI"
                     /locus_tag="IXH04_000790"
     CDS             15941..16867
                     /gene="dnaI"
                     /locus_tag="IXH04_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAQ1851420.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16996..17436
                     /locus_tag="IXH04_000791"
     CDS             16996..17436
                     /locus_tag="IXH04_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAQ1851421.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17580..18281
                     /locus_tag="IXH04_000792"
     CDS             17580..18281
                     /locus_tag="IXH04_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAQ1851422.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            18278..>18545
                     /locus_tag="IXH04_000793"
     CDS             18278..>18545
                     /locus_tag="IXH04_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAQ1851423.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPREKMYAD"
BASE COUNT         6329 a         3250 c         4071 g         4895 t
ORIGIN      
        1 tgaatattgt gtcttgtgac cgaggagttg tcaggagaac accgtttacc agaggttgtg
       61 acttgttttt agtcacgacc tcttttttta tttattccat ataaatacac attatttatt
      121 ccgaaaagtg aaagcgcaaa taacaaaaac aaccaactta tttattatta aagaaaagcg
      181 tatttaagta ggacgattct gttgagagct ttcgtagatt ttcccatttt cctatggtaa
      241 actgacaaag ttgagaatgt tagaacagat cgtttaggag gaaacagatg tttacgaatg
      301 aaataaaaat catgctttat gtggacgatg tagaaaaaaa tgcaacgttt tggagagcag
      361 ttggctttgc ggaaaaagat cgtcaagaaa tggatgggac gcttataatt gaagtaggag
      421 ttgaggacag tcaaacctca tttctgttat atgatcgagc atttgttgag tcccattcac
      481 ctggcacagc tactagtgcc ccatctttga tgttttacag taaagatatc tttggactat
      541 ataaaaaaat gcagacgcaa tcagtcacat taggtgatat ggtacagctt ggagaagaat
      601 atgtctttaa ctttgctgat cccgatggca actactttgc ggttacaggt aaataataga
      661 aataaagact ttgtttttct gtgcgaaaca ggaaagcgaa gtctttttaa ttcaaataat
      721 aatattatgt aaacaaaaaa ctttccatgc gtaattatac acaagcaaat aaatcgtcaa
      781 atgcgaacgc cttctttata ctgtttagga acgaaaagaa ggaaggaaga aatacatgga
      841 ttatcaagta ctgctttatt acaattatac aaaaatcgaa gaaccagagg aatttgccaa
      901 aaatcattta gccttttgca aatctttgga tcttaaaggt cgtattctag tagctgaaga
      961 aggaataaat ggtactgtgt ctggaaccgt tgaaaacacg actgcctata tgaaagcaat
     1021 gaaagctgat cctcgatttg ctgatacgat gttcaagatc gatccaagcg aaggacatgc
     1081 ctttaagaag atgtttgtaa gataccgtcc agagcttgta agtttaaagc tagaagagga
     1141 cattgatcca aaccaagtga caggtgccta tctttcaccg caagaattta aagaagctat
     1201 tttagatgaa cagacagtgg tgattgatgc acggaatgat tatgaatatg acttgggaca
     1261 ttttcgaggg gctgtacgcc cagatatacg cagttttcga gagcttccag actggatccg
     1321 cgagcacaaa gaagaattta tggaaaaacg ggtagtgacc tactgtactg gtggcattcg
     1381 ttgcgagaag ttttctggtt ggcttgtccg tgaaggattc aaagatgtcg gacagctgca
     1441 tggcggtatt gctacttatg gtaaggatcc tgaagtccaa ggagaattat gggacggcaa
     1501 gatgtatgtg tttgatgaac ggatcgctgt cgatatcaac caagtagata aacaaatcgt
     1561 cggtcgcgac tggtttgacg gaacaccatg tgaacgatat atcaattgta gcaatcctga
     1621 gtgcaatcgt cagattcttg cttccattga aaatgaagaa aaacatttag gagcatgtag
     1681 catagagtgt gcgaaacatc cgcgcaatcg ctatgttcaa gaccatggaa tctctccaga
     1741 agaagcagaa ctgcggttta cagctttaat taaatagaaa acaatagttt tgattgtcga
     1801 ccatttccaa tatgcgctat actagcgttg aggatggtga tccacatgaa atcattgatt
     1861 tatgctattg gggatgtcca tggcaaacat gagctttttg aaaaacttat tcactattat
     1921 gatccaaaga tccatcagct tgttttatta ggggatctca atgatcgagg tcctcgtaca
     1981 aaagaatgtt ttttattagg aaagcaatta gtcgaagaaa caggagccat ttacctccgg
     2041 ggaaaccatg aagaatattt tttgcaattt ctgcaatcac cggaggactg gtatgcgcct
     2101 tatatgagaa atggtggaaa agaaacgttt gaaagcctga ttcacccagg ggcatcggag
     2161 gaatactccc caactgaaat ctcgatgatg atccaatcga gatattcaga actgattaaa
     2221 tttttagtcc agcgcccctt gtattatgaa tgggaaaatt atctttttgt ccatgccggt
     2281 gtggatttga caaagaaaga ctggcgtaag acaagtctaa gagactttat ttggataagg
     2341 gaaccatttc atgaagggaa aaacaataca ggtaagacaa ttgtatttgg acatacgatt
     2401 acaccaatgc tccatggaga tatgcagaca acagacctct ggattcagga tcataaaatc
     2461 ggaatggacg gtggggctgt attcggaggg tcattgcatg gggttgtttt tgacaaggaa
     2521 ggtattcttc aagatattga atatcagaac aacgaaggac cttggcatgg aatctgacta
     2581 atggaaagaa tgaaggagct gtgactttgt tgcggctctt tttattttta tagagaatat
     2641 tgtttctttc agtagtttaa cccaggataa aaagtatgat agaataaaga agcaattttt
     2701 agcagaaaag aggcatttta atgacggaaa atttgaacca gacgatcatt cagcttgtac
     2761 aaaaagaact ttcagactat cgtcctaagc agttgactac tgtacttaat ttgttaaatg
     2821 aaggaaacac tgttcctttt atcgcgcgtt accgaaaaga aatgactgga agtcttgatg
     2881 aagtccagat tcgtgaaatc gaagagcggt atgcctatct tgaaaactta gaaaaacgta
     2941 aaactgaagt gattcggcta atcgatgaac aagggaaact gactcccgag ttagaagcag
     3001 aaatcaccca agccgtgaaa atgcaacaag tggaagattt gtatcgtcca tacaaacaaa
     3061 aacgacggac aaaagcaacg attgctaaag aaaaaggact tgaaccactc gcgaagtggc
     3121 tgatgcagct tacagatgga gaggtgcaat ctgaagcaga aaaatatatt gacaaagaaa
     3181 aagaagtctc ttctgccgaa gaagcgttgc acggtgctca tgagattatt gctgaacaag
     3241 tcagcgataa tgcaaaattt cggacttgga ttcggtctta tacgtataac aaaggtatgt
     3301 atgtcagcaa tgtcaaggat gagcaagcag atgaaaaagg cgtatatgaa atgtattatg
     3361 actttgcaga acccgtccat aaaatggttt ctcatcgaat attagctact aatcgtggag
     3421 aaaaagaaga tattctgaaa gtattctttc aagtagatga agcagcaatt ttagcttact
     3481 tggatcgcca aatagtcaaa aatcctgcct ctccttcttc ttcattcgtc agagaagcct
     3541 atcaggatag ttataaacgc tttattcagc cagcaatcga gcgagaactg cgcaatgaat
     3601 tgactgaaaa ggcagatgag caagcaatcg ctattttcgg cgaaaacctc cgtaatcttt
     3661 tgttgcaacc gccactaaaa ggtaaagttg tcctgggatt tgatccggct tatcgtactg
     3721 gatgtaaact agcagtcgta gatgcgacag ggaaagtttt agcaatcgaa gttatttatc
     3781 ctcataaacc tgcagcgcaa gcaaaaagag aagctgctgg accagctttt atccaattaa
     3841 ttaataaata tcaagtagat atggtagcta tcggaaatgg tacagccagt cgggaatctg
     3901 aactttttgt ggctgagcag ttgaaatccg ccgaccataa aacctattac gctatcgtca
     3961 acgaagcagg cgcttctgtt tactccgcta gcgaaattgc acgaaaagaa tttccgcatc
     4021 tacaagttga agaaagaagt gctgtttcta ttgcccgtcg cctgcaagat cctttagctg
     4081 aattagtcaa aatcgatcct aaagctgtcg gagtaggcca gtaccaacat gacgtttctc
     4141 aaaaacgttt ggctgaacaa ttggattttg ttgtggaaac agcggttaac caagtaggtg
     4201 tggatgtgaa tacagcaagc ccgcagctct tgcagcatat atcaggattg aataaaacaa
     4261 ctgcccaaaa cattgtcatt tatcgtgaag aaaacggaga attcaccgca cgaactcaat
     4321 tgaaaaaagt gccacgctta ggaccaaaag catatgaaca agcaatcgga ttcttgcgag
     4381 tccctggtgg gaaaaacatt ttggacaaca caggtatcca tcccgaaagt tattctattg
     4441 caaaagaaat attaacttca gtccaacttt cagaaaaaga gcttggtaca gaagaagcag
     4501 tagaaaaatt gacacgatta tccgtagaga aactggctga aagcctatca gtcggagaag
     4561 aaacgttagc cgatatttta gctgggctga cacaacctgg acgagatatg cgtgatgaga
     4621 tgccagcgcc cttattacgt acagatgtat tgagtatgga agacttgaaa ccagggatgg
     4681 aattgactgg aacggtccgt aatgtcattg acttcggcgc atttgttgat atcggtgtga
     4741 agcaagacgg acttgtccat atatcgaaac tcagcaaaaa attcgtcaag catcctacag
     4801 atgtcgtgtc agtaggagat atcgtaacag tttggataga acaagtcgat acgaaaaaag
     4861 gcagaatcag tttgacgatg ctttcccctt atgaagagta acaatgagga gtaacaaaaa
     4921 ttgacacaag aagaactgca gcagttagta gaagagatat cattaaaaag cttccagctt
     4981 cctttcaagc accaagcaat gttcaatcca cgcttacgta caactggcgg gcgctatcat
     5041 ttaaacgatc atcatttaga ttttaatgcg aagctatttg aagagtcaga cgaagagacg
     5101 attgcaggaa tcatcaaaca tgaactttgc cattatcatc ttcatctagc tggaagaggg
     5161 tatcagcata aagatatgga tttcaagtat cttttgaaaa aaacaggagg gttgcgctat
     5221 gcaccagcct tgacgaagaa acagataaag atggagtatt atcgatgcca agattgttct
     5281 gccacgatct gccgcaagag aagaatcgat gtacgcaaat atcgttgtgg aagatgtcat
     5341 ggagaaatca aatggattga gacaaaaaga gaagacttta atgaagcaca aaaaatcaga
     5401 tgaaaggata gaaggccttt ggaaaaaagt gagcagagga atctaaaaga ctggaatcag
     5461 ctattaaatt ttttagccgc acctgaagag ggaaattttg aggaactgac ggcacctaca
     5521 cttattttag ctggaaactg tcttcctatc ttagctgata aagcagcaca aatgtattta
     5581 aatggccaag tagatcaatt gtttttggtc ggaggggtgg ggcatgcgac tagaatcctt
     5641 tatgagaatt ttgaaaagca aggatttcat tttgaagatg gcatgagtga aagtgaaatc
     5701 tgtcgtcagt atctaaaaga agtctatgat ttgcccgata aggcttttct gatagaatcg
     5761 aagtctacaa attctggaga gaacgccact ttttcactgg agattcttca ttccttagga
     5821 gctgtaccag aaaaaatctt attgatgaat gatccgacac tgcaacgcag gactcgagca
     5881 acttttgaga aagtatggca aaatgaacaa actattttcg cgaatgctgt gccatttgtg
     5941 ccggaaatcc ttcacttttc tgaagagatc atctttacag caaaagaact gaatcatcaa
     6001 tggcctaagg aatatttcca tgcgctagta ttaggagaaa tggaacgtct ccatgacgat
     6061 gagaacggct atggtccgaa aggaaaagat tttattcctc acattgatat atcagaggaa
     6121 gtttggtctt cttacaccag aattaaagag tcaataaaaa cggatttttc tcgtacttga
     6181 ccattttagc aagcgaatcc tcctttccaa ataaatttgg ttgaaatttt caatcgttta
     6241 taagataatg aggagcgtgt tgaatgtaat agctaaaaga atggacgcta gataagagaa
     6301 gaggattcaa atggaaaatc aaaataaatt gtatcatttt gttggtatca aaggttcagg
     6361 aatgagctcc ttggctcttg ttttacatga acaaggtcta aatgtccaag gatcagacat
     6421 tgaaaaatat ttctttacac aaagagattt agaaaaagca aatatcacga ttctgccatt
     6481 taacgcagat aatgtaaaac cagggatgac gatcattgca ggaaatgcat ttcctgattc
     6541 acatgaagaa atccagcgag caaaagaatt aggtttggaa gtgattcgct atcacgactt
     6601 tattggtcat tttatccaaa attatacgag tatcgctgta acaggctctc acggaaaaac
     6661 aagtacgact ggactgctct ctcatgtatt gagtggtgtc cgtccaacaa gttatctaat
     6721 tggagatgga acaggacatg gtgatccgca agcggaattc ttttcatttg aggcctgtga
     6781 atatcgccgt catttccttg cctattcacc agactatgca atcatgacca atatcgattt
     6841 tgatcatccg gattattata caagtatcga cgatgtattt acagctttcc aaacaatggc
     6901 tggacaagtg aaaaaagcta tttttgcgta tggtgacgat gcttatttac gcaaattaaa
     6961 agcaaatgtg ccgatttatt attatggtgt cacagaaaac gatgatatcc aagcacgaaa
     7021 catcgaacgc acaacttctg gttctgcttt tgatgtctat catggggatg aatttgttgg
     7081 gcacttcact gtaccagcat tcggtaaaca caatatctta aatgctttag gtgttattgc
     7141 agtagcttat ttcgaaaaac tggatctgaa agaagttgca gaagaaatgc tgactttccc
     7201 aggagtaaaa cgtcgattca gtgaaaagat cgtagcagac atgaccgtag tcgatgatta
     7261 tgcgcatcac cctgcagaga tcaaagcaac gattgatggc gcacgccaaa aatatccaga
     7321 taaagaaata attgcagttt tccaacccca tacgtttacg cgtacgattg cattgatgga
     7381 tgaatttgca gaagcacttg atttagctga taaagtttac ttatgcgata tttttggttc
     7441 ggctcgggaa gaacaaggaa acgttaagat tgaagacctt ggagcgaaaa tcaaaaaagg
     7501 tggagaagtc atcaaagaaa ataatgtatc tccattgtta gattatcatg atgcagtagt
     7561 aatcttcatg ggtgctggag atgttcaaaa atttgaacaa gcgtatgaaa aacttttaag
     7621 cagcacgacg aaaaatgtac tgtaatacaa ttactgcagt gtccttataa aaatagaaag
     7681 agggggagtg gcaaaaatct tgccaagccc ccttttttgc aacttatgat aaacggtgtt
     7741 caaaagcttg catctgcggt aataagccca atcaaatcaa aaacatgaga agctgttttt
     7801 tcttggctgt tcttattatt tgatgcagga cagctttttc acgacctctg taaactatag
     7861 gaatctgtta aatcaaggga gcagaacttg ccttttacgc tacttttggt acaataatgt
     7921 atgattaaaa ataaactgga gggtttctat cttgaacata ggtaagccga gaaaactagg
     7981 aaaaacaata aatgaaatcg aggaaggcga ctcgttatcc ttaacagaat ctattgaaga
     8041 taaccagctt ttgctgtatt tgggattgac gaatgatgca aatcctttgt atatccaaca
     8101 tgactttgca caaaaaacag aatatcaaca gccaatcgtt ccatcagtga tgctgatggg
     8161 gatcatcaca agtgcaattt caaagcatct tcctggacca ggatcacatg ttgtcaattt
     8221 ttctgtcaat tttattgaac cggttttcca ttatgaaaca ctgacttttc agtttgaggt
     8281 cataaaagta gataagatga aggatgtggt gacgatttct gtagaagcgg agaatacgaa
     8341 cgaagatcgt gtgttggatg ctgtcgttat ggtccaaccg ccacaagttc ctgaattgga
     8401 aatagcaatc gaaagtgaag gtgaattgaa tgaataatcc aagtacatca atgtcaggta
     8461 tcaatcgttt ttatgcaaaa gtttatggat ttttaggtat gggacttgcc cttagtgcag
     8521 tcacttctta cttggctctt tgggtatttc ctttgcaatt agcaactttt atcaataatt
     8581 ttccactagg ctttgtgggt ctgtggatcg ttgaactgat tttagtaatg gttctaggcg
     8641 taaaagcaca aaagaatccg agcttagcaa tcggtggatt tattgcctac tctcttttga
     8701 atggcataac attagcggtt actcttgcat actacgatat tggaacagta acgcaagctt
     8761 tcttgacagc tactttgatg ttcatgggga tgtctgttgt cggagtaaca acaaaaagag
     8821 atcttagcgg ggttggaaga gcaggacagg cagctttaat cggagtgatc attgcactag
     8881 tcttgaacat cttcattttg agaagtagcg tagttgagct gttcatctcc ctactgttag
     8941 tcgtcatctt tgcagggatt acagcttatg ataaccaaag catccgtaat atctatgtcc
     9001 agacaaacgg aacagcacat agtggaattg ctgtattcat ggcattgaag ctgtatttgg
     9061 actttatcaa cttgttcttg gcgtttctga gaatctttgg acggaacaac taaaaaagaa
     9121 aaaaattcat cagttggggc tataccgaat tatttggtat ggtcccgctt tttcttttcg
     9181 cattccgcag gattctttgt tagaatgaat aagaacttta cagtaaggag ttaagaagat
     9241 gacaaaaaat aaattattac tcgttgatgg gaacagtgtg gcttttcgcg cattctttgc
     9301 gcttcacaat tctcttgaac gtttcaaaaa tagaaacggt cttcatacaa atgcaatcta
     9361 tgcattcaat acgatgtttg aaaatgttat gcaaaaagag caaccgactc atgttctggt
     9421 tgcgttcgat gcaggaaaaa cgacttttcg gacagaaatg tatgcggagt acaaaggtgg
     9481 aagatccaaa acacctggcg aattcaaaga acaaatgcca tatatacgtg atttgttgac
     9541 aggactaggg gtacagtatt acgaattgcc caattatgaa gcagatgata ttatcggtac
     9601 tttggcagaa aaagtagaca aagatcaatt tgatgtcgtc gtgctttcag gtgatcggga
     9661 tttgactcaa ttagctagta aagaagtaaa agtggatatc actgtaaaag gtgtcagcga
     9721 tatcgaaagc tatacaccag aacatgtagc ggaaaaatat gatgggttga cgccaaaaca
     9781 gattattgac atgaagggac tagccggaga tgcctctgac aatatccccg gtgtgactaa
     9841 aatcggtgaa aaaacagcta tcaagctatt gaaacaatat ggctcagtcg aaggaattta
     9901 tgaacatatc gatgaaatga agcaaagcaa aatgaaagaa aatctgataa atgacaaaga
     9961 gcaggctttc ttatcgaaaa aattggcgac catcaatact tcagcgcctg tggatgtctc
    10021 tatcgactca ttgaagtatg aagggaaaaa tctcgataaa ctcgttccgt tttataaaga
    10081 aatggatttc aatcagttcc tttctaaatt gaatatcgta gaagaagaag taaaaatgga
    10141 tgatattttg tttgaagtcg ttcatgaatt caaagaggaa atgtttacta cggatatggc
    10201 gttgtacgtg gaaatgatgg gcgacaatta tcatacagag gaaatcgtcg gagtagcgtg
    10261 ggggacagaa aagaaaattt atgtcacgaa cgatctatct gtattcgaaa accgagcgtt
    10321 ccattcttgg atcactgatc cgactcggct gaaaaaagta tatgatgcga aacgaactta
    10381 tgttgcttta aaccgctata ctggaaaaac gaaaggaatc gattttgatg tcttgctagg
    10441 tgcatacctt ctcgatacga atgaaaaaag cacagatatc gaaggaatag ctgctcatta
    10501 cggatacaac gatatccaat cagatgaagc tgtatacggc aaaggggcga aaaaaggttt
    10561 accagaagaa gaggaactct tttttgctca tttagcgcga aaagtagctg caatcaatgc
    10621 gttgacacca aaattgagcc aagaactggc agaaaaaaac caagaagatc ttttccggaa
    10681 aatggaactg ccattgtcta tcattttggc agagatggaa ataagcggga tcaaagtaga
    10741 tgcgactcgc ttacaagaaa tgaagggcga gttctctgct cgcttaagag agatcgaaca
    10801 aaaaatctac gaagaagccg gcgaagaatt caatttgaat tcaccaaagc aattaggagt
    10861 gatcttattt gaaaaaatgg gattacccgt tatcaaaaaa acaaaaactg gatattccac
    10921 agccgtagat gtactggaac agcttcgtga acaagcacca atcgtagagg atatcctcac
    10981 ctatcgtcaa attgcaaaaa ttcaatctac ctatgtcgag ggactgctga aagtgatcca
    11041 atcagatgga aaagtgcata cccgttatgt ccagacattg acccaaacag gacgattaag
    11101 ctctgttgat cctaatttgc agaacatccc gattcgatta gatgaaggga gaaagatacg
    11161 tcaggcattt gttcctagag aaaaagactg gctgatttat tcttctgact attcacaaat
    11221 cgagttacgc gtacttgcgc atatctcaaa tgatgaacat ttgaaagctg ccttcctaga
    11281 aggtcaagat atccatgcca gcacagcgat gcgtgtattt ggaatcgaaa aggcagagga
    11341 tgtcacacca aatatgcgta ggcaggcaaa agcagtgaat tttggtatcg tgtatggcat
    11401 ctctgattac ggactttcac aaaatctagg aatcagccga aaagcagcac agcaatacat
    11461 cgatacgtac tttgaaaaat acccaggtgt caaagaatat atggaaagaa tcgtacgcga
    11521 agcaaaagac caaggctacg tcgaaacgct gtatcaccgc cgtcgatatc tgccagacat
    11581 caattcaaga aattacaata tccgttcttt tgcggaacga acagctatca atacaccaat
    11641 ccaaggaagt gctgctgata ttttgaaaat cgcgatgatc gaattggata aacgattaaa
    11701 agaaacaggt cttcaagcta cgatgctgtt acaagttcat gatgaacttg tcttcgaggt
    11761 accggaaaaa gaacttgaat cattagataa attggtgaag gaagttatgg aacaagctgt
    11821 ttctcttcat gtaccactga tcactgacag cagttgggga aaaacgtggt acgaagcaaa
    11881 ataaacattg tttaagacag agcaatataa tgaaggtgaa aagaaaaaat gccagaatta
    11941 ccagaagtag aaaccgttcg taaaggattg gaacggttag tagtaggaaa aacgattcaa
    12001 aaagttcagg tcttgtggcc caagatcatc gagcaaccag agacacccat ctttgaggct
    12061 tctcttgttg gcgaaaccat ccaatccatt ggacgtagag ggaagttttt aatttttcat
    12121 ctcgatcatt gtgaactgat ctcacacttg cgaatggaag gaaaatatca atttacgaaa
    12181 gaaaatacac caatcgataa acatacgcat gtacttttct tttttgagga tggaagccaa
    12241 cttcgctaca acgatgtacg aaaatttggt cgtatgacaa tcgtcgaaaa aggagcaagt
    12301 gcaacttacc gaggaatcat gaaattggga cctgagcctt tgccagatag cttcttatta
    12361 gcagatttcg caaatggact gaaaaagtcc cacaaagcaa tcaagccatt gctgctagac
    12421 caacgtctag tgactggtct aggtaatatt tatgtagatg aagcattatg ggaagcgaag
    12481 atccatccag aacaaccggc gaacacactg cgccccaaag aggtagaaca attacgtcta
    12541 tcgatcattg atgttttgga tcgtgcaata gaagccggag gtaccacgat tcgcagttat
    12601 ttgaatgcat taggagaatc aggcggattt caagtagcac tgcatgtcta tcagcaaact
    12661 ggcaaaccat gtatacgttg tggaacgccg atcgtgaaaa caaaagtggc acaaaggggg
    12721 acgcactatt gtccgaattg tcaaaggcta aaaatcgcaa agtaggattt gttttaggtt
    12781 tgacgggtgg aatagctacc ggtaaaagta cggcagccaa agtctttcag tctcatggat
    12841 ttcctttgat cgatggcgat gtgattgcca gagaaactgt tgagcctgga acgcttgctc
    12901 tgaggaaaat tgtatcagtg tttggccaag aggtattgca gcctgatggg caactagatc
    12961 gagggaaact tgggatgatt gtttttccat caaaggaaaa acggcaaaaa cttgatcagc
    13021 tattagatcc gtttatccga aaagcgataa aagatcaaat cgcctctcta agttccaatc
    13081 atcctttagt aattgtagat atcccattgc tctacgaagg tcattatgac cactatatgg
    13141 atgcagtagc tgtcgtttat actacgccag aaactcagct tcagcgtttg atgaaacgta
    13201 atcaactgac gctagaacaa gcgcagcaga gaattagtag tcaacttcca atcgaagaaa
    13261 aaaagcagcg tgcggatatt ttgttcgaca acaatggaac aaaagaaaaa cttgtgggac
    13321 aaatcgaaaa ttggcttttt gctaatcgct ttctttgagt atctgtgtca attttgctat
    13381 cctgagtata tagaaagtga gggatagcga tgggatcatt aaaaaatcaa aaggataaag
    13441 tcgtaggtaa cgcaaaagaa aaaatcggcg agatgctcaa taaagaaaat ctaatcgatg
    13501 aaggtcgtac gcaatcagaa acagcagaag aaaaagaaaa gcagtaccaa gcaagaatcg
    13561 atgaaataga agaccagcag gcagaagaag aagcgaagcg gcaagatttg actcaagaac
    13621 gaaaaaatcc gacacctgat cgtagtaaaa caccgctccc agaaagtgac cacattcccg
    13681 atgagcaaaa aatcgaggaa cagcgaatga aacactatac aggaatcgaa gagaagttat
    13741 aaaatagtaa aaaatcgaga tgtgacttga agcagatgag aaggtgttca tctgcttcaa
    13801 gttttttgtg ctagatgact aaatcagctt ttgtgtaagt tatttctcag taatcaaggt
    13861 gatcagtatt ggaaaattga aatttagtaa ggacagggga cacaattacg catgaaagta
    13921 gaaaaaattg ttcttatatc cgtgtttttt gggtaggaca gtcgcgtttg tgatttgttt
    13981 cgtgttataa tacaactagt tgtaaagaaa gacacatcct ttatataaaa tgcgatgcaa
    14041 gaggtgagac aaaatgagat gtcccagatg tcaacataat aattcacgag taatcgacag
    14101 ccgtcaagca gatgatggac gtgcgatccg tcgccgtcgc gaatgtgaga attgttcgtt
    14161 ccgattcact acatttgaac gaattgaagc tgctccgtta ttagtaatca agaaaaacgg
    14221 ggaacgcgaa gagttcaatc gcgataaaat tttaagagga ctcatccgtt cagcagaaaa
    14281 aagacctgta gctatggaac aaatggaaca aatcgtcaac catgtcgaaa accgcgtacg
    14341 cagtctcgga gaaaacgaag tatcgacgaa tctaattggt gaatatgtga tggaagattt
    14401 agttgattta gacgaaatcg cttatattcg ttttgcaagt gtttatcgcc agttcaaaga
    14461 tatgtctgtt ttcttaaaag agttgcaaga tatcgtggat aaagcaaaag aaaataataa
    14521 agagaataat aaagaaaaca attaagaaac aaaaaggagg catttccttg gaaaatgctt
    14581 ggaaagagct tcaaccaaaa aatatttacc aaggaagaaa agcggctccg ctgagagaag
    14641 acagcaagaa cgccttgctg tatctttatc agccactaat cggcggagaa gccttgagta
    14701 tctatttcac cctgttatca gaaatctctc aagaatctgg caaaggtcct gaagggatgc
    14761 atgcagatct gcttagtagt ctaggctgcg gtatcccgca attttatgat gcaagaaaaa
    14821 agctagaagg aatcggctta ctggatgttt attttaaaga agatagtgat ctgggacctc
    14881 gttttattta tgagctgtta gaaccactca ttcctaaagc atttttcaaa gactctgttc
    14941 tggcttttct tcttctggaa aaagttggtg aacgacgatt ccagaagctt acccaattct
    15001 ttgaacctaa aaggcataca gcagagggat atcaaaaaat cacaaaaaaa tttttagatg
    15061 tttatcgctt caatgaagca gtgtacgctg ctaatcaaga aaacatcgaa tcgctgaata
    15121 aatcttttga agaatctgaa tcagtagcat tacttgaagc agattcgaca ttcgattggc
    15181 agtttttgaa tgattggctg gcaaaaaaac atattgcaag tcttgatgat ccaatcatca
    15241 aacaagtcga catgtatcat cagctttacg gtttcgatga attgacactt ggcgaactga
    15301 tcagtcaatc ttatgacttt agtgaacaaa aagtatctga aaaagaactg caaaaagcca
    15361 ttttggcaat gcagagacgt ccggtgaagc aaacaaaaga aacagttgcg gtttctgaaa
    15421 acagtaatga accctcgtca aacgaacatt tgacaaacgc agcgcttcag ctgataaaag
    15481 aagcacagag cattccaccg atgaaatatc tggaagcaat caagtcagaa aaaggcggat
    15541 atgtttctaa acaagaaaac tggttactgc aagatcttgt ttctcaatca ggtctttcca
    15601 gtagcgtgat caatgttttg ctgaattatg tactagtgat caagaataat gctagcttaa
    15661 atgccagtta tgtaaataca gtggcaaatg aatgggcaca aaagaaaatc gccacagcag
    15721 aagaagcgat ccagcacatt cggcaaataa gtaatcaagc aaaacaatca aaacaaaaga
    15781 agcaaacaaa ttatcaaact ggcaagcgaa atgttcgacg ggaaaagctt cctgattggg
    15841 tcaaccagcc aaaagatgaa aaacagatca gcagcgagaa aaaagcagag attgaccgcc
    15901 gttttaaaga atacctggca aaaaaggagg gtgatatcta gtggaagacg taggcaaaga
    15961 attgaagagg atcatcagca aacgcaatta tcaagaaaag tatgctgaaa tgatcgctga
    16021 agtcttaaat gatgcggatg ttcaagcttt tctaagcgca caccaagaag aattgacaca
    16081 agcggatatt gaaaaaagtt acgctaaatt gtatgaattc gtgcaagaaa agcgtaaata
    16141 tcaaaaaaac gatccaacga tgatcgctcc cggctatgag ccgaagctga cgttgaattt
    16201 tcattatgtg gatgtcactt atgtaccaac ggaagcattg ttagcacggc aaagacaaga
    16261 agaaatcaga agccgagtaa aagcaatgga tatgccaaaa gatattcaat cggcttcttt
    16321 tgaaaacttt gaacgtacag atggacgagg atatgcgagc ttagaagccc tagattttgt
    16381 agagaaatat actgctgatc caaaagggta tcataaagga ctgtatctag taggaagttt
    16441 tggtattgga aaaacatatt tattaggagc aacagcgaga gaattagcga ttgctggatt
    16501 caccacgacg ctagtccatt ttccgacatt tgccgttgag atgaaacagg caatcggaaa
    16561 agatcaagtg gcagaaaagt tagatgcagt gaaacgttca cctattttga tgatcgatga
    16621 cattggtgca gatgcgatga gtagttggat acgagacgaa gtattcggtg ttatccttca
    16681 atatcggatg caggaacagt taccaacctt cttctcatcg aattttacaa tggatgaatt
    16741 ggaacagcat ttgtccgtca ctcaacgtgg agatgaagag ccgctaaaag cgaaacggat
    16801 aatggaacgc atacgttatt tgacaaaaga aatcgaaatg actggtcgca accgacgcaa
    16861 tggttgaaca gttgacaagt ccaactgaca tttgacctag tagtaaaagc aggtcagcga
    16921 atcattatat ggacagaaat gaccggagac aatcaccgca acaagtaatg cagaaactta
    16981 aacggaggat tttttatgag ggctattttt tctagtatat ctgtagttgg tctatttttt
    17041 cttttagcag gctgcggtag cgcagatagc aacacaggtt ctgttgattc gattgatgcg
    17101 ggtcaaacag taaaaacaag cagtacgaaa acggagagac aaagcatatt tgtcggaacg
    17161 attgaaagta ttactcaagt agaagcagaa gaagaaacgc ttcagttgaa actcgtcgat
    17221 gtcatggcca aagaagatcc agcgaatatg ggaacgtcat tcagcaatga cggagtgatc
    17281 ttaaatgcta ctgctgaaaa aataagcgtg gacataaatg aattgaaaat cggtgacaaa
    17341 gtccaattta cattagttga gatgccgatc atgacgatgt ctatcccacc gcaagttcct
    17401 ggagattcga ttattgatgt ttcattgaac aaataaccaa taacattttt tcaaaaataa
    17461 gaaaatgttg ttttatagaa cgaaaaggta tattttttaa ggagatatgg gaattttttt
    17521 attcctagtc tccttatttt atttggaaaa tgctataatt atctagaagg agtgaagcta
    17581 tgaaccattt agatttatct cagcgcttag ccgcagtggg tgactttgtc cctgaatccg
    17641 ctcgtttagc agacattgga tcagatcacg cttacttacc cgttgcattg atattaagag
    17701 gaaaaatcga atatgcagtt gctggtgaag tcgttcaagg tccttatgaa tcagcacgta
    17761 agcaagtcaa aaaagatgga ttagaagacc gtatcaccgt acgccttgca aatggtctag
    17821 atgctatcca gccacaagat ggtattacag ctgtcacgat tgcgggtatg ggaggttcac
    17881 tgatccgaga tatcctagaa gctggcaagt tgaatcaacg cttaagcgga caagaacgtt
    17941 tgatccttca gccaaacatc ggagagcgta cattacgatc ttggctgcaa gaaaatcagt
    18001 ataaaatcca atcagaaaaa atacttgaag aaaataaaaa aatatatgaa atcattgtag
    18061 ccgaaaaaga acaaaaacca gttacttaca atgaaatgga gctgatgttc gggccatttc
    18121 tgcttgaaga gaaaagtgag atcttcaaat caaaatggac aagagagctt caacaaagaa
    18181 aagctatccg tgaacagctc aaacaagcag ctaatcaaga ggcacgtcaa aaacaaatcg
    18241 agcaggaaat ccaaatgatt gaggaggttt taaagccatg acaatcagcg gacgcacttt
    18301 catccaaaag tttgaaagct attgtcctca gtggctagct gaagaaggag acccagtcgg
    18361 actgcatatc ggaacattgg ataagccaat ccaacgagta atgatgacat tagacgttcg
    18421 tccagaagtg gtcgaagaag caatcaagaa aaaaatcgat ttgctgattg ctaaacatcc
    18481 tccaatcttt cgtcctgtaa aacggctggt tactgatcag ccacgagaaa aaatgtatgc
    18541 agatt
//
LOCUS       DACEXQ010000029        16650 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.029, whole genome shotgun sequence.
ACCESSION   DACEXQ010000029 DACEXQ010000000
VERSION     DACEXQ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.029"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..642)
                     /locus_tag="IXH04_000794"
     CDS             complement(<1..642)
                     /locus_tag="IXH04_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851424.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYI"
     gene            complement(821..1540)
                     /locus_tag="IXH04_000795"
     CDS             complement(821..1540)
                     /locus_tag="IXH04_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ1851425.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(1671..2822)
                     /locus_tag="IXH04_000796"
     CDS             complement(1671..2822)
                     /locus_tag="IXH04_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAQ1851426.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(3039..3779)
                     /locus_tag="IXH04_000797"
     CDS             complement(3039..3779)
                     /locus_tag="IXH04_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1851427.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYQYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(3783..4106)
                     /gene="rsfS"
                     /locus_tag="IXH04_000798"
     CDS             complement(3783..4106)
                     /gene="rsfS"
                     /locus_tag="IXH04_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAQ1851428.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(4163..4756)
                     /locus_tag="IXH04_000799"
     CDS             complement(4163..4756)
                     /locus_tag="IXH04_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1851429.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(4740..5387)
                     /locus_tag="IXH04_000800"
     CDS             complement(4740..5387)
                     /locus_tag="IXH04_000800"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAQ1851430.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(5400..5711)
                     /gene="yhbY"
                     /locus_tag="IXH04_000801"
     CDS             complement(5400..5711)
                     /gene="yhbY"
                     /locus_tag="IXH04_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAQ1851431.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(5727..6836)
                     /gene="yqeH"
                     /locus_tag="IXH04_000802"
     CDS             complement(5727..6836)
                     /gene="yqeH"
                     /locus_tag="IXH04_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAQ1851432.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(6833..7360)
                     /locus_tag="IXH04_000803"
     CDS             complement(6833..7360)
                     /locus_tag="IXH04_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAQ1851433.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(7540..8325)
                     /locus_tag="IXH04_000804"
     CDS             complement(7540..8325)
                     /locus_tag="IXH04_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAQ1851434.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(8318..9187)
                     /locus_tag="IXH04_000805"
     CDS             complement(8318..9187)
                     /locus_tag="IXH04_000805"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAQ1851435.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(9188..10567)
                     /locus_tag="IXH04_000806"
     CDS             complement(9188..10567)
                     /locus_tag="IXH04_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAQ1851436.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(10575..10994)
                     /gene="fabZ"
                     /locus_tag="IXH04_000807"
     CDS             complement(10575..10994)
                     /gene="fabZ"
                     /locus_tag="IXH04_000807"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ1851437.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(11019..11495)
                     /gene="accB"
                     /locus_tag="IXH04_000808"
     CDS             complement(11019..11495)
                     /gene="accB"
                     /locus_tag="IXH04_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAQ1851438.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVISEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(11498..12733)
                     /gene="fabF"
                     /locus_tag="IXH04_000809"
     CDS             complement(11498..12733)
                     /gene="fabF"
                     /locus_tag="IXH04_000809"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAQ1851439.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(12752..13489)
                     /gene="fabG"
                     /locus_tag="IXH04_000810"
     CDS             complement(12752..13489)
                     /gene="fabG"
                     /locus_tag="IXH04_000810"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAQ1851440.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(13501..14418)
                     /gene="fabD"
                     /locus_tag="IXH04_000811"
     CDS             complement(13501..14418)
                     /gene="fabD"
                     /locus_tag="IXH04_000811"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAQ1851441.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEATPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(14525..14749)
                     /locus_tag="IXH04_000812"
     CDS             complement(14525..14749)
                     /locus_tag="IXH04_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ1851442.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(14802..15764)
                     /locus_tag="IXH04_000813"
     CDS             complement(14802..15764)
                     /locus_tag="IXH04_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAQ1851443.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(15774..16214)
                     /locus_tag="IXH04_000814"
     CDS             complement(15774..16214)
                     /locus_tag="IXH04_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1851444.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            16520..>16650
                     /locus_tag="IXH04_000815"
     CDS             16520..>16650
                     /locus_tag="IXH04_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ1851445.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFH"
BASE COUNT         4423 a         3770 c         2834 g         5623 t
ORIGIN      
        1 tatatataaa tccaaatctg tttcaggata ttttaatttg acaagcgaaa taatcatttt
       61 cagttcttcc atgttttctg gatattctgg gtcaaatacc ttgctatgac ggccaatccc
      121 gccattgtcg atcaatcggt aaattcgatt ccattcaatg aattggtcaa ttgattggaa
      181 attgctgatt ggatagccgc cacaatccaa aatctctgat acacgaaatg ctaggtatgc
      241 actatcgatc actttatttt ttttcgtgtc tatcttctca tttctttcga taagaatttt
      301 ttcaggacga tgtttcaagc tccactctaa tagtagaaag aaagcctcca tccgatactc
      361 ttcttcaaat aaccgaaagg ctgaatcaga acgacgaaaa agcagcggaa tcagataatt
      421 ttgtctataa atcagcattg gtgcgcggtc caccatacac tctaaataac ggttcagata
      481 ccctgtaccg acatagtttc ctaatcgata gccttcaacg ccgaatcttg cgtatttttg
      541 cgctaatttt gaacaatcag ttacgttcac agctaactct tctactttgc ggtaaccgct
      601 taagatacgt tcaaaaggtt ctaaagtgat ttttgcttcc atattcttcc ccgtccatct
      661 tccacatact tcatatcatt tcacatgata cttatgctct catcatacca caggttgaat
      721 tagaaaagaa aataaatcac acaaaaaagc aagacaatct gttagtgaga ttgtcttgct
      781 tttcgaaaaa gactgaatac ttttcgttaa aactggttgc ttagtcttct aatgccacat
      841 tatggaacac atgctgtaca tcttctagat cttccagtgc atcaatcatt ttttcaaatg
      901 tttcttgatc ttctggagat agtgttacct cgttttgagg gatcatttgg atctcagcga
      961 ttgaaaaatc agtgattcct ttttctttta atgcttcttg tatactatgg aaatccgcta
     1021 cttcaccgta aacgatgatt tgtccttctt cttccattac atcgcgaaca tcgatgtctt
     1081 tttccattag gtattcaagg atctcgtcag cgtcttctcc tgcaaaagca aagattgctg
     1141 tattgtcaaa catgtaagaa accgctccgg aaacacccat gttcccgcca tttttcccaa
     1201 atgctgcccg tacgtctgct gctgttcgat tcacattgtt cgtcaatgta tcaacaatca
     1261 ccattgagcc gcttggtcca aatccttcat agcgcaattc agagtatgtt tcatctcctg
     1321 caccttttgc tttttcgatt gctcggtcaa taatatgttt tggtacatta taagttttag
     1381 cgcgatcaat gacgaaacgt agtttttggt ttgcatgcgg atcaggatca cctgattttg
     1441 ctgccgcgta gatttcaatc ccaaattttg cataaatccg gctattatta gcatctttag
     1501 cagttttctt tgctacgata tttgcccatt tacgtcccat attttcactt cgctttctga
     1561 tttgatagtt tcataacgca aaaacgtcct gttccattat acttgtgaat cacgattctg
     1621 tgaagctttt tttctttctt ccttcattct tctcattttt agtcaattat ctatactcgg
     1681 attgggattt gcccgaaatt ctgttccgga atattgcctc ccaattgata gatttgatca
     1741 cttctaacta acagttccgc ttcttgtgcc tctttttttc cgattcggga ataaatagaa
     1801 atctcttttt tttcttttaa gaatcttctt ccagcatcgg taaatccaag tagtcgaaga
     1861 taatttttat tttgttggac aggaatctct tgctttttga tattcagcaa gacataacaa
     1921 gataatcgtt ggatccgtgt ccatgtatat ctttttgttt ttacctttga cacaaaatcc
     1981 tcaaaactat cagcagttgc agctgctgtt ttcatgcgcg attctattcc ttcggtcatc
     2041 tgatagatat ctgtcagttc cagcaaagta gaagataaca accgataacg caaatacggc
     2101 caataagatt cccaagtctg cagcgaacaa gttgaaagat cttcttctgt ctgaggcggc
     2161 acaagtccag ctattctttt tccttcttct aacgcatggc gaatggctgt tgcactggct
     2221 atcctctgat ctgtgatgat tttttcatga tagcctgcac ttttacgtcg gatcggatgt
     2281 aattccatag ggtggacata cttggcattt tcctttgcat aacttaatcc caagatatga
     2341 tttggcgaag aaaaatcaag cgtaatatcg ggatacagac tgcgaaaaac ctcagtcatt
     2401 ttttgcggat aactatacgt ttgatcagac agttcttgaa atgcctcttc tatcctatct
     2461 tgattctctt ggacaaagcg tccgaaactg ctgtaatcaa acgaagtctc ttcttctgtt
     2521 ccaaaacaca aactgtcaca gtccagtgtt tgcaagattt ttactgcacc tttagcaaaa
     2581 tagtcagctg actgaaccgc ccattcgacg ggaagctcca ctactaaatc cacaccattt
     2641 tgtaaagctt cattcgcgcg atgccattta tcgatgatag ccggttcgcc tctttgcaaa
     2701 aaattcccgc tcattacagc aatgacgacc tctgctcctg ttatttttcg tgccatttcc
     2761 acatgatagc gatgtccatt gtgaaaagga ttgtactcta cgataatccc gcagcttttc
     2821 atgcgcatcc tccttcatag ttatcgataa aaaagaaata gagacaagta aaaaaaggca
     2881 aaaatcggaa aaaatacccg aactatataa actattctcg caaacacaat acttctgcga
     2941 cgaaaagctg cactgcaaga gcaatgccta tatcgttcgg atatataatt tatgttgaag
     3001 atttattctt ttcttgttaa ctcgagcagg caaaaccgct agtctttgta tgctacaaag
     3061 aaccagcgct ttgatttctc tgatgctgct tcgtctgtaa aatcagaaaa tgcttcgact
     3121 ttcgaaaagc cgctgttttc cagcattctt aaataacttt ccaatggata ggtacgctct
     3181 tgatgtagtt catcttcacg aataaatcga ccagaccctt cttgttcttc aacgaaaaag
     3241 gtaagaaaat gttcgatgct gtgtttctcg cttcctggat aactatccca caaaaaagca
     3301 aaattatccc cttgatattg atagctatat tctggaaaaa cctcatctac ttgataggtg
     3361 gagtgtacgt caaataaaaa tcggccattt tcttctaacg cttgatagac ttcatcaaac
     3421 acttgctgta cctcttgtct atttttcata tagcacagcg aatcagaaaa acaggtaatt
     3481 gcctgatatt gtccgatttc tgataaatcg agcatattgc cttccacaaa ttggatcgat
     3541 gcgtcttctt catatgctcg ctggctggca atcatcaaca tctcttctga aaggtctaac
     3601 cctgtcactt tatatccatc ttttgcaaag gcaaccgcta atgctccagt accgcatgcc
     3661 agttccaaaa tattttttgt gtcttcttgt aaatgacgtt tcgaaaaagc taaccattgc
     3721 tgataaagtg tttcgtccat cacttcatcg taaatgaaag caaatgtttc ataagccata
     3781 tgttaatcca accattcact aagatcgact agcggagcat ctgaccatag tttttccaga
     3841 ttatagaaat ttcgttctgg cgcatggaaa acatgtacga tcacatctcc aagatcgatc
     3901 aatacccatt ttccgccttc ttttccttca atacgtttta cttcatattt gttttcttct
     3961 tctttatcaa tgatttcttc tgtaattgcg ttgatttgac gttcgctatt tgctgaacag
     4021 atcatgaaat aatctgccaa caaggaaaca tttcgaacgt ctaaagcgat aatgtcttct
     4081 gcccgttttg aatctgctgc ttcaatcgca atttttaatt gttctttact atctatgatg
     4141 atttcctcct aatatgagca gcttattttt ttgctaccca gtaattatat gtttcgattg
     4201 tttttggata aatctgttgt tcttgcgcaa gcaaatgggc caatgtatgt tttgtttcaa
     4261 atgctacagc ttcgtctaga tcagcccatg caatggctcg tgcatcttgt accccaggaa
     4321 aatctcgacc tggttcaata tagtctgcta cataaatgat tttgtcaagg agactcatct
     4381 tagcagcccc agtcgtatgc acccggattg catgaagaat ctctgcatcg gtgattccta
     4441 attcccgctc tacaaacgaa gcgccaacaa gaccatgcca gattgcatta ttataattca
     4501 ataactccgg atcaaaaccg tctcttacga ttaccatttt gaactcctca tctggtcgtt
     4561 cttttgcata atcatgtgtc aatgcagcaa tactagcttt ttcaggagaa gcaccgtatt
     4621 ttttggcaag agccacggct gtttcttcta ctccaagcac atgcttgaag cgttgttcgc
     4681 tcattctcat ctgtattttt tgcatcaggt tttctctaga ataagcagta tattcattac
     4741 tatagtccat attcatacaa cccttcattt tgaatataat caattacttt atctggaaca
     4801 aggtagcgga tagaacagcc ttctttgatc ttttgacgta ttttcgtgga actgatgtcg
     4861 atctcaggta catctaccca aattacagga tatggagtat ccgttgtgta tcctggtcga
     4921 cgtataccca caaagttgac catagaagtc agctcatcga ttttatacca ttttggcaaa
     4981 tattctacca tatccccacc gataataaag taataatctg tatccggatt gttttgcgtc
     5041 aattccttca tagtatcata ggtgtaactt ttcccaccac gagctaattc aatcgtttca
     5101 atctgcaaaa aaggattgtc ttccaccgct aattccaaca tgttgagtcg atgttttgca
     5161 tcaatcgtct gtttctcatc aacatgaggt ggctgataag aaggcatcaa aaatacgcgg
     5221 tcaagaccta aattccggcc agcttgttca gccatcacta aatgtgcaag gtgaacggga
     5281 ttgaatgttc cgcccaagat ccctacttgc tttttcttct ctaagaattg aggcatttct
     5341 tcaggaaata attgtgaacg aacaaatgtc ttagcttgcg tattcatcat ggtcactcct
     5401 tatattgctt ttacttcttt tgatagcctt tgatactttt cttttgtgga aggtttgtat
     5461 aatacaataa cacgtccaat gatttggacg atttcgcaat ctatttcttt ttctaatact
     5521 tgagctgctt cttctgcaat ctcatctgta ttttgaagta acgatacttt gatcagttct
     5581 cttttttcta aagcttcttc aatttgaacg atcattgctg aattgatccc accttttccg
     5641 atttgaaaaa tcggctgaag atgatgcgct ttgctacgta aaaaacgctt ttgttttcct
     5701 cttaaactca taattttcct tcttccttaa atcaatgctt tgcgtttcaa tacatctaca
     5761 ccctttggcg cccagcccgc aattactact ggcgctgtta cagtgatcca gcctagaccg
     5821 gcaaaaacga tgtctgtttt ttctttcaca gaaaactcaa aacgaacaag ctccggaaat
     5881 gcctcgactt catcaggacg aggaggctgt aataatccac ctacatgttt ttgataaaat
     5941 tcgtctgctt tttccagttt cgttcgatga atatttacat catttgacac ataagcaata
     6001 aatgaagcac gattgccgct gataaaatca aatcttgcaa gtccgcccaa gaaaagtgtt
     6061 tgcccttcat tcagctgata aactttaggt ttgatttctt ttaacggtgc aatgatccgc
     6121 aaatcttttt tacctaagta gtgcgccatt tgatggcgat ggataattcc cggtgtatca
     6181 atcaaaaaat gcccgtcatc taaaggaatc tcgattttat ccaaggtcgt tcctggaaac
     6241 tgcgaagttg tgattacatc ttgtactccc gctgtatttt tgatgatttg attgataagc
     6301 gtcgattttc cgacatttgt tactccgacg atatagacat cccgtccatc gcggtattct
     6361 tcgatttttt ctaatagttc cgtcatctca tgagatttct tagcactagt taataataca
     6421 tctactggac gaattcctgc ttcgtaagca cgttccttca tccattgtgt taatttagtt
     6481 tttttcaatg atttaggtaa tatatccact ttgttcccaa ctaaaagtac tggattgtca
     6541 ccgacaaaac ggtgtagtcc tgggattagt gaaccattga agtcaaaaat atccaccaca
     6601 ttgacgatca atgcatcttt ttgccctaat tcattcagta agcgcaaaaa gtcgtcatcc
     6661 gtcaatgaga catcttggat ctcattataa tggcgtaatc tgaaacaacg ctgacaatag
     6721 acttccccgg aagctaaacc tttttccaaa gccgtttgcg gcgtgtatcc taattcaccg
     6781 ggtttgtttg tttgtataac ggcaccacag ccgatacatt gtaattcttc tgtcattcga
     6841 ttccacctcg ccagcccata tcagggtgtt ttgctaatag ataggccata attctttttt
     6901 cccaaaaacg attaaattgt gtcttccaac tatctgttgc gataatgggt tttacaagga
     6961 tgctacgtat ccctgcacga ttggctccac gtatatcagt cataatctga tcgcctacca
     7021 ttactatctc atcatctgct aaatccaatt gtttctttgc tatgttgatc cctcttgcta
     7081 aaggtttcat tgctcgtgca acatacaaaa gatcaaattt ttcaatcgct cgcgcgacac
     7141 gttttgaatt attgtttgaa acaacaacaa cagggattcc tgcatttttc atttctaaaa
     7201 tccaagtaag caactcttct gttccatctg gattattcca tgcaatcaac gtattgtcta
     7261 aatcggtcaa tactgctttg atgcctagat tctttaattg ggcaggtgta atctgataga
     7321 tggcttctac catccatgtt ggtttgtaat ttgaaaacat gctgtctcct catctgcaaa
     7381 aaatgaagga gcacataaag tactcctcaa ccccgtatta tactgcatat cagcaaaaaa
     7441 aactacctct atactcgttc gtctttcaga aaaagcattt ctttacaaaa tgaccaaaaa
     7501 taaagattga tcacagaaaa atttagaaac aaaaaacaac taatattggc ggaacctctt
     7561 gtatcgtttt tccagtaatt tatgctgatc taatcgaaca agttccgtca atctttcaat
     7621 caacgctttt ttcataatgt gtgcgacttt atatcgtggc aactgctcgc cgttgaacgt
     7681 ttcaggaata actcgatcaa cgattcctaa ctcgcgtagt tctggcgcag taattttcat
     7741 caactcagct gcttctgcgg cgcgactacc gtctttccac aaaatcgacg caaatccttc
     7801 tggagaaagc acagcataaa tcgtttcttc cagcatccag acttcatccg ctaaacctag
     7861 agcaagggca ccgccacttc caccttcacc aatgatgatg gcgatgatcg gtacctttaa
     7921 atcagacatt tcgagcagat ttctggcaat agcctctcct tcgccgcgct cttctgctcc
     7981 tacaccgcaa tatgcacctg ctgtattgat caaggtaatc accggtctat tgaacttttc
     8041 cgcttgtttc atcagtcgta aagcttttcg atagccttca ggatggggag aaccaaaatt
     8101 tctgcggata ttttcctgca gatcccgtcc tttttgaata ccgatcactg ttaccggttt
     8161 gccatctaat cgagcgattc caccaacaat cgcttcgtca tctgcaaata aacgatcacc
     8221 atggcattcc tcgaaagaat cgaagatctc ttcaaaaaaa tccaagcttg tccatcgttc
     8281 tttagctctt gctaactgaa cgacatcatg tgctgtctta tccacgatct atccatcctt
     8341 tctgtgtatg caattcaaga agcgtatgga ttttctgttt caattccatg cggggaacaa
     8401 tctgatcgac aaaaccatga gataataaga attcagcctt ttggaaatct tctggaagtt
     8461 cctgcttgat cgtctgttcg atgacgcgtc ttccagcaaa accgatcaag ctttggggtt
     8521 ctgctagaat aatatcgcct tccattgcaa aactggcagt tactcctccg gtcgtcgggt
     8581 ccgttaaaac agttagataa aaaagacctt cattgctatg ccgtttgaca gcagcagaga
     8641 tttttgccat ctgcattaga gaaaagattc cttcttgcat ccgtgcgcca cctgaagctg
     8701 tgaacaagac gactggaaga ctttcctttg ttgctcgttc gaataatctg gtgatctttt
     8761 ctccgacaat cgttcccata cttcccatga taaaattagg atccatcact ccaataatga
     8821 agggaatccc ctgaatggtt gctttaccag tgaggactgc ttcatgaagt cctgttttat
     8881 cttgcatttt tttaattttt tccgtatatt cgggaaatgc tagcggatct ttggttacaa
     8941 gatccgtatc ccattcttca aaactttttt catcaataat caaagctaga cgctgccatg
     9001 caccgattcg aaaagcgtat ccgcaatgag ggcatacttt ttcttcaccg atgtctttcg
     9061 tataaaggat atgtttgcaa tttggacact ttgcccacat gttatcagga acagatggcg
     9121 agttagcagg tttgctctct ctattaggat tgatccgaat atatttcttt ttcttaaaca
     9181 gagccatcta tgcgcctcct atctccgttt ctggtgtcca attcggtaaa aattcttctt
     9241 gtaaaaatgc tgtgctgtaa tcacctgcta tgactgcagg atgactaatc aaatccatct
     9301 gaaattctgc gttcgtgatg attccttctg tgataaattc acttaaggct cgctgcatct
     9361 tcattaatgc ttcaaaacgc gtatttccat ggacgattat ttttgcaatc attgaatcat
     9421 aatatggcgg gatcgtatat cccgggtaaa ctgcactgtc tactcgcagt cccatcccac
     9481 ctgctggaag cagtaagttt tggatcttcc ctggagaagg agcaaaatgg aaagcaggat
     9541 tttctgcatt aatccggcat tcgattgcgt gtccttggaa tactacttct tcttgacaca
     9601 cattcaaagg ctcaccggct gctatctcca cctgtttttt tactaaatcg attcctgtga
     9661 ccatttctgt tactggatgt tccacttgga ttcgagtatt catttccatg aaataaaaat
     9721 cgccttcttc atccatcaga aactcgatcg ttccggcatt ttcataattg actgctttag
     9781 ctgctcgaac agcagcgtct cctagtgctt ctctttttgt ttgtgagatc actacagaag
     9841 gagattcttc cagtactttt tgattgttgc gttgtaaaga acaatctcgc tcgcctaaat
     9901 gaatgacatg accaaatcga tcacctaaga tctgaacttc tatatggcga gcaggataaa
     9961 tgattttttc caaatacata tcatcatttc caaatgccgc tgctgcttct tgctgagctg
    10021 aagaaaaatg ctgtgggagc tcttcttttg attgaacttt ccgaatgcct ttcccgccac
    10081 cacctgcagc tgctttcagc atcactggat aaccaattct gtctgctatt tccaatgctt
    10141 cttctactgt agataataca ccagtacttc ctggaatgac tgggacattt gcttcttgca
    10201 tcaattgacg cgcattgatc ttatttccca ttgcatcgat cgttgcactt ttaggaccaa
    10261 taaatgtgat attgcactct tcacacatgg aggcaaatcg gctattttca gataaaaaac
    10321 caaatcctgg atgaattgct tcggcttttg tcacgattgc agcgctcagt acttcctgaa
    10381 cattcagata tgaatcaacg gcttttgccg ggccaatgca gattgcttca tctgctaact
    10441 gtgtatgaag tgcttcggca tctgcttctg aatatatcgc tacggtttgg atccctagtt
    10501 cgcggcatgc acgaatgata cgtacagcga tttcaccgcg gttagctatc aatactttag
    10561 aaaacatatt tcacctatcc aatcataaag gttaattccg cttcggctac ttttttaccg
    10621 tctactttag cgattccttt gcctattccg gctgaagctt tcactttcaa tatctcaact
    10681 tcaaggtata aggtatctcc cggtgtaact ttcttacgga attttgcttt gtccaatccg
    10741 ccgaaataag ccgtctttcc cttgaattgt tcaagagaaa gtaatgcaac tgctcctgct
    10801 tgagccattg cttcaacaat caatacccct ggcataacag gttcttctgg aaaatgtcct
    10861 tgaaaaaagg gctcattgat cgttacattt ttcttagcaa cgatccgttc tccttcaacc
    10921 atttcttcca ctcgatcaat caaaagcatt ggataacgat gtggaatgat ttcttttatt
    10981 tcttgtatgt tcattgtttt ccctctttcc tgctttgctt atttttgctg aatcctaaag
    11041 agtggctgtc catattcgac aactgactcg ttttctacaa ggatttctgt gatcactccg
    11101 tcggcctcac tagttatctc attcatcagt ttcatcgctt caataatgca gataacttct
    11161 cctttggcaa cttgatcacc tacttttttg aaagctggtt tgtcgggcgc tggttgcaga
    11221 taaacgattc ccacgattgg cgaaacaacc tcgattccat caaatactgc cgattctttt
    11281 tctttcatta tttctggagc agttgttgct tgttcagata tgacattgac tggctccgaa
    11341 actggcaacg tttcgtttgt ttgaaccata ttcatgcctc gacttgtttt atttttattc
    11401 atatacaatt caaattgacc ttcacgcaaa tcgaattccg ttagcgagga ttgatcaaat
    11461 tgactgacta attctctgat ttcattgata tccaaaatta ttcctcccag cgtttcatgc
    11521 acagaaccgc attatgtccg ccaaacccta atgaattact cattgcataa gtaaaacttg
    11581 caggtttgct ttcgtttttg acaactgtca agtcaatctc aggatcttgt ttttctacat
    11641 tgatcgtagg tgggataaat tgatgttcta atgccagcaa agttgccaag gcttcgatcc
    11701 cgccggctgc tcccaataaa tgtccagtca tcgatttcgt gctgctgacg taagtatctc
    11761 tgaaattttc acctaatgcg atttggattg cttttgcttc tgcctggtca ttggctggtg
    11821 tacttgttcc gtgagcattg atatatccga cttgttccgg ctgtacacca gcttcttcca
    11881 tcgcaagctt cattgcttta gatgcaccag aaccatctgg agtaggagct gtcatatggt
    11941 aagcgtcaca gtttgcaccg tatccaacga tttctccata gattttggca ccgcgagctt
    12001 gtgcatgttc taaagattca agaactagaa cgcctgctcc ttcacccatc acaaaaccat
    12061 tccgattttc gtcaaacgga atcgaaccac gattaggatc agttgattct gagagtgctg
    12121 ttaaagctgc aaatccagca atccccatct cacaaatcgt tgcttctgtt cctccagcaa
    12181 gaatgacatc agaatagcca tgtttgatgt tacggaatgc ttccccaatt gaattcgttc
    12241 cgctagcaca agctgttacg atagctgtac aaataccttt tgcacctaca cgcaaagcaa
    12301 tatttccagc tgccatgttg gcaatcgcca ttggtacgaa cattggagaa actcgttttg
    12361 cacctttttc atgtagacga ataacttggt tttcgattgt tggaagacca ccgatcccag
    12421 atccgatgat gacgccaaac cggtcttgat tcattttttc tttatctaaa ccgctcatct
    12481 ccattgcttc taaagcagcg tagattccat agatcgaaaa catatccatt cgtttgctat
    12541 cttttttaat aaaatattta tcgtaaggga aatctttgac ttcacccgct aaggtaatcc
    12601 ctgtttctga agcatcaaat ttagtgatcg gtgcgatgcc attcgtcccc tcttttaagc
    12661 tattcaaaaa gctgtctgcg tcatttccaa taggtgaagt gactccatag ccagtaatta
    12721 ccactcgatt catttgtttg ctctcctttt cttatccgtg catgaccaat ccgccatcga
    12781 cattgatgac ttgtccggtg atatatggat tttgtgctaa gaaaactgct gtttgtgcaa
    12841 tatcttctac ttgtccgaaa cgctgcatcg gaatttgctt ttccgcttgt tccttgactt
    12901 tgtctgctaa aacttccgtc atatcagttg tgatgaatcc tggcgcaatg gcattacatg
    12961 taatgcctct agttgcagct tctcgtgcca cagattttgt taatcctact acgccagctt
    13021 tactagctgc ataatttgct tgaccaatat ttccaatcaa cccagataca ctagagagat
    13081 taatgatggc cccttctcgc tgcttcatca tttttttcaa tacgtgctgc gtcatattga
    13141 aggttccaat caaattgata tccagacatt ttttgaaatc ttctgcatcc attcgcatca
    13201 caagtttgtc atttgtgatt ccggcattgt ttactaacac atggatcgat ccaagctttt
    13261 cttctgcttc tttgatcatt tgtcccgctt tctcataatc agagatgtca ccggaaactc
    13321 ctacgcattt tacaccgaat gcttcgattt cttctatctt ttcttttgga atctctccgc
    13381 gaccattcaa aatgatattt gctccagctt ttgcaaaagc taaggccatc gctttgccga
    13441 ttcctcttgt actacctgtg ataaatacat tttttccttt tacatccata acgttcctcc
    13501 ttatagtatt gctattgttt catcaaatgt ctgcttattc tcaacacgca gaactggtat
    13561 tgttttatcg atttttttca taaatccgct taatactttt ccggggccga cttcgatgac
    13621 ttgttcgatg ccgattgttt ttaacttatg gatactctca tagaaacgaa caggttgcat
    13681 gacttgctgt tcaagtagtc ccgcgattgt ttctttcttc attatttccg ttgttgtgtt
    13741 gcttatgata ggaaaagaag gttcagagaa ttgaatctga cttaggtcct tcgccagttt
    13801 ttttgctgcc ggttctaaaa tagctgtatg aaaaggcccg ctgacattta gagggatcat
    13861 tcttttgaac cctttttctt ttaatagtaa aacagcttcg tctactgctt tttcttcgcc
    13921 accaatcacg atctgctgag gtgtattata atttgcagga gatacgatgc cgtacttgct
    13981 agcttcatga cagctttcct cgatcgtttc aatcggcgca ttcatcactg caaccatttt
    14041 cccgcttcca gctggggtag cttctgtcat ataagcgcct ctttttgcta ctaacgcaac
    14101 tgcttcagtg aaggatagcg cattactagc tactaatgcc gaatattctc ctaaactcag
    14161 tcctaaagct gcatctggga tgattccatg ctccttcaat aagcgataat acgcgatgct
    14221 gactgttaga atagctggct gtgtatattg cgtctgatcc aaacgttcat tttctgtaaa
    14281 gcaaagttca gccatatcgt agccgagaat ctcacttgct tcatcaaatg tctgctttac
    14341 aacggcttct tcatataaat cttttcccat accttgatat tgtgcacctt ggccactaaa
    14401 taagaatgct gttttcatgt ttttctccaa tatatccaga atatatgttt gattcttcct
    14461 attcaaaaag gccgagacat tcctatttca ggaaatgtcc cagcccccaa cctaatttta
    14521 atgattatgc agtttgtttt tctacgtaag tcactaaatc ttgaaccgtt ttgattccgt
    14581 cttctgtttc gattttcacg tcaaattcat cttcaatttc attgatgatt tggaataaat
    14641 ccaagctgtc agcttctaag tcttcttgga tgtcagtagt caatttgatt tcttcagttt
    14701 cttttcctaa ttcttctgcg atgatttctt gaattttttc gaataccata ataatatatt
    14761 cctccgatat tgtttgcttt ttatttttat aatttaacag tctacaactc catgagaata
    14821 cttccccatg ttaaacctcc gccatagcct gtaaatatta ctttctgctg ttttcctaag
    14881 gtgatctttc cactagaaac tgcttcatcc aataagatag ggatgcttgc agcagatgta
    14941 ttcccatatt tatccatatt ggtcaggaac ttttcttgcg gcatctttac ttttcgggca
    15001 attttctcaa taattctgag gtttgcctga tgcaaaagca gataatcaac tggtttgtct
    15061 ttcatgactt cttggatatt gtttgcaacg tctcgtacgg caaaatcaaa aatatctctg
    15121 ccagtcattt gcaaataata cgaaccttct gaatcttctg aatagaacgg atttttattt
    15181 tgctggtagc cagcggtcaa tgagctactt ctggtaccgt cagctgctaa tttttctttc
    15241 agcaattttt tctgtggatt cgcttctaag agaatcccac ctgctccatc gccaaacaac
    15301 acagcagtcg tacgatcaga ccaatcgatt agcttagata gtgtttcacc accaatcacc
    15361 agtcccttag ttgagccgca gcggatcatt ttttcagcca tgcttaacgc ataaacaaat
    15421 cctgagcacg cagcactgat atcaaaagcg atagcatgag ttgctcccaa tttcccttgt
    15481 acttgtgctg ctacagatgg tgtcgtgtaa tcgggcgaca ttgttgctac taaaataaaa
    15541 tccaaatctt tagggtttag atttttctgt tttatcaatt tttccgccac taaaatgcat
    15601 aaatcagaag tctcctgatt tgttacacat cttcgttcac gaatacccgt ccttgacgaa
    15661 atccattcat cagaggtatc catcatcttg gaaagatcat tattagtaat aatcttttca
    15721 ggaaggacgc tggctgttgc agtaatcatt ccaaattgtt ccattaaata tcctcactta
    15781 tagtcttgca agaagtcgtg caagtttttc agtgcatcca gcaaggcatg ctgttcctcc
    15841 gtagccattc cgtctaagat gttcttgacc atttcacgat gaaaatgctg atggacccga
    15901 aatagtaact ttcctttttt cgttagtccc aactttacaa cgcggcggtc atcttcactg
    15961 cggatacgtt cgacatagcc tttttttact aacttgttaa tagcagtggt caaggtccca
    16021 acagtaattg atagttcttt ggctacttct gaagttgttt ttttcttgta catcccgatt
    16081 gcctcgatcg tgtgcatctc tgtgattgac agatctttga attgagattt tttcagctca
    16141 gactcttcga ttgtcaaaat atcattgaag acactaacaa gataatcatt tactgttttt
    16201 aaatctggtt ccatgttcca ccgtccacta attagtttga ttatcaaatc ttttgatgtt
    16261 caaactatat cttaactttt ttcaaaatgc aaccttaaaa gatcattttt ttactatatg
    16321 agcttttttt aagattttac tcatttaaaa caactgataa atcagcattt ataatttctt
    16381 ttattagaaa aatcatctat ctcacatatt ttgattatca aagtattttc tgtgaacaga
    16441 actatagaat tccctaaaac ttgtccatta tttcaaaatt tgttatagtc tttttattca
    16501 aagcgtaaaa ggagatccta tgttaaaaga aaaaatgaaa actctcccca tgctgccggg
    16561 tgtctatctt atgaaagacc gagatggtaa ggtcatctat gttggtaaag caaaacggct
    16621 caaaaataga gtaagcagct attttcatca
//
LOCUS       DACEXQ010000030        16489 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.030, whole genome shotgun sequence.
ACCESSION   DACEXQ010000030 DACEXQ010000000
VERSION     DACEXQ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.030"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            144..3836
                     /gene="nifJ"
                     /locus_tag="IXH04_000816"
                     /pseudo
     CDS             144..3836
                     /gene="nifJ"
                     /locus_tag="IXH04_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
     gene            4025..4912
                     /locus_tag="IXH04_000817"
     CDS             4025..4912
                     /locus_tag="IXH04_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAQ1851446.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            4909..6078
                     /locus_tag="IXH04_000818"
     CDS             4909..6078
                     /locus_tag="IXH04_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851447.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            6102..7457
                     /locus_tag="IXH04_000819"
     CDS             6102..7457
                     /locus_tag="IXH04_000819"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAQ1851448.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(7529..9334)
                     /gene="glmS"
                     /locus_tag="IXH04_000820"
     CDS             complement(7529..9334)
                     /gene="glmS"
                     /locus_tag="IXH04_000820"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAQ1851449.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            9743..10033
                     /locus_tag="IXH04_000821"
     CDS             9743..10033
                     /locus_tag="IXH04_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851450.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            10046..11071
                     /gene="sppA"
                     /locus_tag="IXH04_000822"
     CDS             10046..11071
                     /gene="sppA"
                     /locus_tag="IXH04_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAQ1851451.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            11074..11733
                     /locus_tag="IXH04_000823"
     CDS             11074..11733
                     /locus_tag="IXH04_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAQ1851452.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            11890..12822
                     /locus_tag="IXH04_000824"
     CDS             11890..12822
                     /locus_tag="IXH04_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851453.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            12976..14265
                     /locus_tag="IXH04_000825"
     CDS             12976..14265
                     /locus_tag="IXH04_000825"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAQ1851454.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            14512..15762
                     /gene="clpX"
                     /locus_tag="IXH04_000826"
     CDS             14512..15762
                     /gene="clpX"
                     /locus_tag="IXH04_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAQ1851455.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            15845..16432
                     /locus_tag="IXH04_000827"
     CDS             15845..16432
                     /locus_tag="IXH04_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAQ1851456.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
BASE COUNT         5444 a         2943 c         3638 g         4464 t
ORIGIN      
        1 atttagactg tttgtctaaa aattagatga ccaattcaaa tagatacatg taaaatattt
       61 ttataaataa aagaaaaaaa tggcatgttt tacgttataa tggaacagat aataagagag
      121 aatatgaaca aggatgatga atcatgcgaa aaatgaaaac aatggatggc aatacggcag
      181 ccgcatatat ttcttatgca tttaccgaga ttgctgcaat atatccaata acacccagtt
      241 cgaccatggc tgaattagta gatcaatggt cagcagcagg aaaagaaaac gtattcggtc
      301 aaccagtaaa agtagtagaa atgcaatccg aagcaggagc agctggtgtg gtccatggat
      361 cattgaaaac tggtgcactg acaactacat ataccgcttc acaagggctg cttttgatga
      421 taccgaatat gtacaagatt gctggggaac tactgccaag tgttttccac gttgctagtc
      481 gggcagtgac gacaaatgcg ttgaatattt tcggagatca cggagacgtg atggctgcaa
      541 gacaaacagg atttgctatg ttagctgaaa gcagtgtaca agaagtgatg gatttagcac
      601 ctgttgccca tcttgcttct attgaaggag aagtcccttt tttgaatttc tttgatggat
      661 tccgtacgag tcacgagatt caaaaaatcg aagttttgga atatgaagaa ttagctcctc
      721 tgatcaatca ggagaaatta gcttcatttc gtaaacgagg gatgaatccc aatcatccga
      781 ctgttagtgg tacgaaccag aatccagaca tccattttca acaacgggaa acgatcaacc
      841 cctattatga taaattacct gaaatcgttc aaaaatatat gaaagagatc aaccgattac
      901 gaggaacttc ttatgacttg gtgacttatt acggagcaga cgatgcggaa gaagtcattg
      961 tttctatggg ataagttgca cagacgattg aacaaacgat tgattacttg aatgcaaacg
     1021 gacggaaaac aggttttttg aatatccatc tttatcgtcc ttttccagtg gaaacattcg
     1081 tgcaaaagtt gcctcataca gtaaaaacaa ttgctgttct tgatcgaagt aaagaaccag
     1141 gggcaggtgg agaacctctc ttattagacg ttcaaagtgc gttatatgat tctgaacttc
     1201 gcccggctgt tattggtggt cgctacggat tggggtctaa agatgtgact cccgaccaaa
     1261 tctcagcagt ttttgatgaa ttgaaaaaag acccaagcat acgcaaaaaa cggtttacca
     1321 tagggatcgt ggatgatgtg acttatcaat ctttggaacc aaaggaaagt ctggatttga
     1381 cagaaccgca aacattccag gccaagtttt ggggatttgg atctgacgga acagttggag
     1441 caaataaatc agctatcaag attattggag atcacaccga taaatatgcg caaggctatt
     1501 tttattatga ttcgaaaaaa tctggcggtc tgactgtctc tcatcttcgt tttggagata
     1561 cgccgatacg ttcagcgtat cttgtcgagc atgcggacct tgttgcctgt cacacaccag
     1621 cttacctgca tagctatgac ttggtcaaag gattgaagcc aggtggcatt tttttattga
     1681 atactctttg gagcgacgaa caattagaaa cgcatctgcc cttaaaatta aaacgttact
     1741 tggctgaaaa taacattaga ttctatacga tcaatgccat gagattggcc caagaagtcg
     1801 gtttgggacg ccggatcaat acggcaatgg aaacggcttt ttttaaatta gctgacatca
     1861 ttccttttga tgaggtgctt ccgctgctaa aagaagaagc cctgaaaagc tatggacata
     1921 aatcaatggc tatcgtagaa aaaaacatac aagcaatcga tcggacgacc gaactgcttc
     1981 atcaagtcgc gatcccagac cattggagaa cgttggagat gcctgaaaaa atcaataaaa
     2041 ggaacactgg ttatatacaa gaaatacttg aaccagtgaa tgcacaggaa ggaaatcaac
     2101 tctctgttgg tacacttgta gcaaatggaa tgacaactgg cgaaatgcct ttaggaacgg
     2161 cttctatgga aaagcggggc gtcgctttag aagtacctga gtggattagt gatcgctgta
     2221 cgatgtgtaa tgaatgtgcc tttgtttgtc ctcatgctgc catccgtcca tttttagcag
     2281 atgaagagga aatggaagaa gcgccagaag gatttatcgt gagagaaatg cgaggcgcag
     2341 acggcgtaaa ataccgtatc caagtttcag tggaagactg tacaggttgt ggtctgtgtg
     2401 tagaggcttg tcccgcaaaa gaaaaagctt tagtgatgaa gccttatgaa gaacagaaag
     2461 aacaagctgt gaattgggct tttgcaatga cattaagaca aaaagcaaat ccagtcaaag
     2521 gaaagaatac tgtcttgtct acacaattca atcaaccgct tcttgaattc tcgggcgctt
     2581 gctcaggctg tggcgaaaca ccttatgtaa aattattgac acaaatgttc ggtgatcgaa
     2641 tgatgatcgc gaatgcgaca ggctgttctt ctatttgggg cggtgcttct cctgcttctc
     2701 cttatacgac caatgaatgt ggtcaagggc ctgcatggtc aaattcttta ttggaagata
     2761 atgctgaatt tggttatggt atgcttctag cggcacagac tcgtcgcgag gctttggcaa
     2821 gttcaatcaa agaagtattg ccaaaagtat cagaagaact acgagaattg atggaagact
     2881 ggttagtaca catgtttgag agtgaaggaa cgcagcaaag agcagcaaaa ctgcaagctg
     2941 cgatcgaaag cgaacgatca attttccctg aactggagga tatatacaag agaaaggatc
     3001 tattcgtaaa aaacagccaa tggatgattg gtggagacgg atgggcttac gacattggtt
     3061 ttggcgggat cgatcatgta cttgccagcg gagcagacgt gaatatcctt gttttggata
     3121 acgaagtcta ttccaatact ggcggtcaaa cctcaaaagc aacaccagct tcggcaatcg
     3181 ctaaattttc ggctagtgga aagtatgctt caaagaaaga tctaggaatg atggcgatga
     3241 catatggaaa tgtttatgtg gcgcaaattg cttcaggtgc caatcaaatg caaacgatca
     3301 aggcatttga agaagcagaa aagtatcctg gtccttctct gattattgcc tatacgcctt
     3361 gtatcacaca tggcttgctt ggcggaatga aagaatcgct taaagaagca aaagaagctg
     3421 tttcttctgg ttattggtca ttatatcgat ataaccctac aatggtcgaa aaaaacaaga
     3481 acccaatgac tttggactac aaaaaacctg atttctctgc tatgcctgat ttcatgagga
     3541 aacaagttcg cttttcctcg ctagataacg caaatccaaa tcttgcaaaa gctttatttg
     3601 aaaagactgt ccacgacgct aaaacacgtt tttacaacta tgctgcactg acgggagaat
     3661 tagaaaaaat caagaaacga cttgaaccag aggaatctat cgatgtaccc gttaagacta
     3721 gaaaagaaaa aacgacggac cttgaagcag aagaaagaag agctgctaga agagctgcca
     3781 gagcagaacg acgtaaaaaa atggaacaag ctacaaaaga aaaaaaggaa aaataacaga
     3841 aaaaatataa tgaataattc tctgaagttt aaaaatattt attgcagaga aaaataaaac
     3901 gcatacttgc aattttcttg aaaattatgc gtttttctca agaaaaatac gagcatatca
     3961 gcctctaaaa ttaaaacata tgatatacta gagaggaaat ctcaaaagag aagagaggct
     4021 ttttatgtca tttcaaatcg gggaactttt taatttggac tattggcagc aaatgttttc
     4081 cggtgacttg ttctctctga attttttggt gaacatactt gatattctgg ttgtctggta
     4141 tcttgtatac aaactgatcc aactgctgcg aggcacaaaa gcgattcagc ttctgaaagg
     4201 tgtagctatt tttattgtta tccgaatcgt agcggaaatc attggtctgc acaccttgtc
     4261 ttggctgatg aaccaagtca ttacgtacgg ggtgatagct gctatcgtga tttttcagcc
     4321 ggaaatccgg agaggactgg aacatcttgg acgcagttcg cttttcaaac aatcaaaaag
     4381 cgaacagcag gaagatgaga agatgatctt gtcttttgac aaagccatcc aatatatgtc
     4441 caagcgaaaa atcggtgcgt tgatcactat cgaacgtcat actggattag atgagtatat
     4501 tgagacaggg attgctttag atgcagatat cactggggag ctgctgatca atatctttat
     4561 tcctaataca ccgctgcatg acggtgcagt tattgtaaaa gaaggaaaga tcgcagtagc
     4621 aagcgcctat cttcctctat cagaaagtat gttgatccct aaagaattcg ggactcgaca
     4681 ccgggcagct gtcggtataa gcgaagtcag tgacgcgatt accatcgttg tctctgaaga
     4741 aaccggggac gtcagcatca cgttagataa tgaactgatg gctggcttat cccagcaaga
     4801 atatttggca atcttgcgta gagaactaat tgctgaagag aaaacaaaaa acaagaaaaa
     4861 cgtgattcaa tctttcctag acggtatgac taagggaggg gatcgcaaat gatctcaaaa
     4921 gagaaacgga caagcattct ttacagtttt attgcattgc tgttcagtat tgcgctgtac
     4981 ttcaatgcaa atggccagtc cgttccaaat acgctttctg gaaatgaagc gtataatcaa
     5041 acagttacgg gggtgccgat caagatttct tatgattcaa agcgctacta tatccatgga
     5101 tatgaaaatg cagtgactgt caaactttcc agtgcaaacc gagttcagtt gaatgcagag
     5161 gcaaacgaag atacacggat gttccgcgtc acagcagata tcactaagct gggcgaagga
     5221 acacatgaag ttccgctaaa aattcaaaat ttgagcagtg ctgtcacagc aaagatcgaa
     5281 ccatcgacta ttacagtaac ggttgagaaa aaggtaataa aagatttcga tgtggaaacg
     5341 ttgattcctt caacgttcac accatctgga tatgaacttg atgatacttc cgttagtcct
     5401 aaaaaagtct ctatcactac aggtgacaaa acattggcag aaatcaaacg agtagtagct
     5461 gatgtggacg cttcgatggt cacagatgat gggattaata gtgaggtccc aatacaggcg
     5521 ctgaatgcag caggggaagt attaagtatc gtctctgatc cagtgcaagt aacagtgaga
     5581 gctgattcga tcaagccaag caagtcggta cgactttatg ggatacagca aggaacacca
     5641 gctgccgggg tagaaagcta tgatttcagt ttttcaaaac tggaagcaga agtaagcggc
     5701 tctagcgatt tgcttgcatc gatcggtgac agtattgctg taccgatcaa tgtctcaggt
     5761 atcaatcatc gaacaactcg taaaattgaa ataccagtag aagaggggtt aagtatcaag
     5821 cccgaatcag ttagtgtgga gattacaccc gtgttgcaaa cgtcaagcag tagctctgct
     5881 tcgacgagca gtcggacaca agaaaataca agtacgagta gaagtatttc atctgctgat
     5941 tcaggaacgg aaccatcgac aacaaccatt tcttccagtt cggaatctgg gacaagcagc
     6001 tcgaccacaa gtacagaaga aacatcagaa acaagcgaat caacacaaat gagcagaaca
     6061 aattcctcag aaaattaagt taaaggagta attttacaac tatgggtaaa tattttggaa
     6121 cagacggcgt acgcggtgaa gccaacaagg aacttacgcc agaacttgca tttaagctgg
     6181 gacggtgcgg aggttatgtc ctaagccagc atgaagaaga aggaaaacgc ccacgcgtgt
     6241 tagtagggcg ggatacaaga atctcaggtc aaatgttaga agaagcgctg atttcaggac
     6301 ttttatcggt gggtatcgaa gtgttccagc taggtgtgat ctctacacca ggtgttgctt
     6361 atctgacacg tttgcaaaaa gcaagcgcag gggtcatgat ttctgcttct cataatccag
     6421 cacaagataa tggaatcaaa tttttcggtg cagatggttt taaactagta gatgaccaag
     6481 agctagaaat tgaagcatta ttagatgctg aagtggatga attgcctcgt ccatcagctg
     6541 aaggattggg aactgtagaa gaatttccag aaggcttatt gaaatattct caattccttg
     6601 tacaaacgat caacggtgac ctttccggtt tgaccatttg tgtcgatgca gcaaacggag
     6661 caacttcaac aagtgttaat cgtttgtttg ccgatcttga aacagatttt tatacaatgg
     6721 gcacaaatcc agacgggtta aatatcaatg acggcgtagg ctctacccat ccagaacgtt
     6781 tagctgagat ggtcgttgaa aaaggagcag atgctggact agctttcgat ggcgatggcg
     6841 atcgtattat tgcggtggat gaacttggaa acattgtcga cggtgataaa atcatgttta
     6901 tctgtgctaa atacttggca gaaaaaaatc gattgaaaaa agatacgatc gttacgactg
     6961 tcatgagtaa cttaggattc cacaaagcgg tggaaggaat cggtttgaaa gatgtcgtta
     7021 cacaagttgg tgatcgttac gttgtagaag aaatgcgcaa aaacgattat aactttggcg
     7081 gcgagcagtc tgggcacatg attttcttag attttaacac aactggagat gggatgcttt
     7141 caggaattca gttactgaat atcatgaaac agacaggtaa gaaattatca gagcttgcag
     7201 cagaggtaac aatctatccg caaaaattag tgaatatccg cgtaagtgat aaaaacggtg
     7261 cgatggatgt tccggcaatc aaaaaagtga ttgaagaggc agaagctgaa atgggatcag
     7321 aaggaagaat cctagttcgt ccttccggta cagaaccatt acttcgagtg atggcagaag
     7381 cgcctactga tgagaaagtt aattactatg tcgataaaat cgcggcagta gtcaaagaag
     7441 aaatcggact ggattaagag atattttcat aaaaaataaa agaacaagga ccagtgtcag
     7501 atgtctgaca ctggtccttg ttcttttatt attctactgt cactgatttc gctaaattac
     7561 gtggtttgtc aacgtcgaat ccccgtaata gcgttgcgta atatgcaagt aattgagtcg
     7621 gtacgactgt ggttagagta gttagataag ggtggacagt tggtagcacc agctggtcac
     7681 tttccttttc taatccctct gagacaataa ctagtgtttt agcgccacgg ctttcgactt
     7741 ctttcaggtt tcctcgagta tgcgcaccag tcacttcttc actgatgatt ccgatcactg
     7801 gtgttccttc ttcgatcaaa gcaatcgttc catgtttcaa ttcgccagcg gcaaaacctt
     7861 ctgcctggat ataactgatt tctttcaatt tcaaagcagc ttcaagagaa acgttgtagt
     7921 cagcaccacg tccaatgtaa aaggcatttc ttgtatcgct gagatatact tctgccaact
     7981 cttctaaata atctttttgg gcgatgaccg attccatcgc attagcaact aaactcaatt
     8041 catgagtgat atcgaataca agagaagttt caaattcttt tttatcaccg atagctttgg
     8101 ctaataaagt tagtacagct atttgtgcag tataagcttt cgttgatgct actgcgattt
     8161 cgggtccagc atgcaatagc agtgtaaagg atgcttcccg cgaaagagta gaaccagcaa
     8221 catttgtaat cgtaagtgaa gggtagccga gacggttgat ttttaccagc acttggcggc
     8281 tgtctgctgt ttcgccactt tgactcaaga aaataaagaa tggttttttt gaaagaagag
     8341 gtgtgttata gccaaattca ctggctaaat gaacctcaac aggaatctgt gtcaattttt
     8401 ccaaagtctg tttacctgct aaccccgcat ggtaactagt tccgcaagca acgatataga
     8461 ttcgatcgct atcagacaat gtatcgacca gcttttgatc aagagagaca cgattgtctt
     8521 ctgttacgta tttttgagcg atacgacgca tgataatagg ctgttcgtcg atttctttca
     8581 gcatataaaa aggataggta cctttctcga tatctgaagc atctacttgt gcttcgaatg
     8641 aagaacgttc tacgatgtca cctgcaaaat tttgaataat aactttttct gatgtgacgg
     8701 taaccatctc ttcatcttca atctcaacga attccttcgt atgagcaagc atagccattg
     8761 catcgctggc aatcacattg aatccatcgc cgagtccgat taatagtgga cttttatttt
     8821 tagcgacgta aataacatca ggtgctgtcc gatcaatcaa agcgaaagcg taagaacctt
     8881 tgatgacccg caaagctttt aaaaacgcct cttgcgttgt agtatccttc gcgaaagttt
     8941 caattagatg agcgataatc tcagtatcgg tctcaccaat aaagtggtca tttgctaaat
     9001 aagcaatttt aagttcttca aagttttcga ttacgccgtt gtgaacgagc acgaagcgtc
     9061 cactttggga agtatgtgga tgggcgtttt cttcagaagg ttgcccgtga gttgcccatc
     9121 gtgtatgacc aatacctata tttcctgtag tatcaatatt tattttgttt ttcaaatttt
     9181 gaattctgcc ttgtgctttg accagataat cagttgtgcc atctgagaca aagataccag
     9241 ctgaatcata accacggtat tctaattttt ctagaccgtc aatcaatcct ggtgttacat
     9301 tttttaatcc aatcattcca acaattccgc acataaaaaa catcctactt tctataatga
     9361 attcgtatga atgcttccag ctgcaggaaa atccttttgt tttatctttg cagacatttt
     9421 ttgtggattt tctgtagagt gcagtcccta gggccatccg ccgaatcttt cgataaaccc
     9481 tctcctcgtc atctgtttac acagacctgg cgctatccta aaaggtataa ctatcctcct
     9541 ttttgttttt tatctggaaa catttaaaat cataaccttt tgcgaatgtt cttgtcaaca
     9601 aaaaaagaat aaaaaataaa ttggtatata tcaaattctt tgtcaaatgt cctttttatg
     9661 tcaaaaaata atgggttttt atctatcatc tttttcagga gttcggtaca atagaaagta
     9721 atcggataga ggaggagtaa aaatggattc aaaaattgag atcatgacac tgggaatgtt
     9781 aaaaaaacaa ttgtccaaat tcgaagcatc agctggtgta tcagatgata cgaaaatatt
     9841 tttagatact ggctgggata gcatccaaga aatcgcacct gatgccttgg aagttgctca
     9901 agctcgtgag tttacggtag aagatgaatt aacaaaagag tcgttttccg ggtatgcgcg
     9961 ggaagaaaaa gcagagcggt tcgacacttc tgagcagtca gaaactgtga tcgtcataaa
    10021 aaacttgtat tagaaaggag aaagaatgaa taaaagacgt tgggcagcag ttctgattgc
    10081 tgcaggatta tttgtggttt cgttagtttc agctagtttg actaagcctc aggaaaacga
    10141 tcaaattgag ggattgaaca gttggttata tggggatgaa gaactagctc caaatgtgtt
    10201 ggaagaggga gacagtacgc aaaaaatcgt aaaattatca gtagatggaa cgattgctgc
    10261 gggagggtct tcaggtttat ttgcttctga aggttacaat cataaaaatt ttatggaaca
    10321 actgaaggcg attgaagaag atccaacagt caaaggtatc tttttagaag taaattctcc
    10381 tggtggcggt gtctacgaaa gtgcggaaat cgctaagaaa ttggatacga ttcgtaaaga
    10441 acatgatatc ccaatgtatg tcagcatgaa aaacatggca gctagcggtg gctactatat
    10501 ttctgctcaa gcagataaaa tctttgcaac agaagaaaca gtgaccggct caattggtgt
    10561 gatcatgtca ggattgaatt attcaggact tctagaaaaa ctgggtgtgg aagatacaac
    10621 agttaaaagc ggagcgttga aagatatggg ttcaggaacg cgaccagaga caaaagaaga
    10681 agaagcggtg cttcaagcat atatcgacag cgcatatagt cgttttgtag caatcgtcag
    10741 cgagggacgg aataaatctg aagaagaagt caaaaaaatt gctgatggaa ggatttatga
    10801 tggtgtccaa gcaaaagaag taggtttagt cgatgaattg ggatttcctg aagatgcttt
    10861 aaaagctata cgaaaggaac aacagcttga agatgcggaa ctagtagaat attcttccac
    10921 gccatctggg tttgctagta catggttagg aagtaaacta gcagaattcc agggattgaa
    10981 gccttctgag actagtcaga tcttatccgt tcttgagaat ctagggacag cggaatcacc
    11041 aaaagcaatg tattattacg gaggtgagta agcatgtcag agctttcaaa tgaaaataag
    11101 gaacaagaaa aagataagca acagcagcct gctgtaggac ctgactccaa cttttcccaa
    11161 aaggtactga gctcttttga aagagagcct ttgacgcccg aagaaatcaa aaagaaacaa
    11221 cgtaaatgga aaaagtatac ggacacaaga aatgaaccgc caaaagcaaa tgattttcct
    11281 aagtatttct atgcaggttt ttttatccga atgtttgcct tcttggtcga tcttttgtgt
    11341 atcggagcta ttacgcgttt aacacttggt attgccaaaa atctaggttg gcttaatttt
    11401 tcagattctt atctgagtct ctacggattc acagccttac ttatttatct agcgtatttt
    11461 attttattga cgaaattcaa tcatggccaa acgattggga aaatgatctt tggtattcga
    11521 gtcatcagct ttgatgaaga agaacttagc tggtcaaccg ttttagttag agaaggtgct
    11581 tgccggttca ttctaaaatt tcctttgctg atgttagggt atcttccagc agtctttaat
    11641 cagaaaaagc aacatctggg cgattttttc actaatacaa gtgttgttac gatcaatctg
    11701 atcaaagcgt tcaaccagga agtcaacagc tgatgagcaa taaaaagcag taacagttga
    11761 aagcaccttc taaaagtcag atctaaaatc tagcttttag agggtgcttc agttattttt
    11821 aattgtcatg ctttttttgt tttgctacta ttgaacatga gctgaatatg agcaagagga
    11881 ggacactgcg tgaagccgat tgattttcca gaaaaatatg agaatttaat gcgagtggca
    11941 caacaagcac tagcaaacca gcaatatcag caggcaaaag aattatttca acgagcttac
    12001 gagttgaaag aaagctttga ggctaatagc ctgcttgttt tttgtcttta cgagttggac
    12061 gagaaaaaag aagcattgaa gcaagcactc ttgcatgaaa aacagtatct tcaaaatgag
    12121 gagtttgctg aattttattt tgacttgtta atcagtacac aagatttttt atatgctaga
    12181 aaattgatag caagtacaga tttttatgaa gctttcgagc agcgggtcat tgaaaaaatc
    12241 caatttgcag aagaattatc cggacaaatg gagaggcaga agatcaaggc actccacaat
    12301 aaatcggagg aacttccttc gttggaacct gcacgtcagc ttgcgtctat tgatgagatc
    12361 gaacagcttc cttatcatga gtttatccaa acagcgagta aacttattgt atcgccagag
    12421 gtccatattt tagcaagggc aaaattactg gagacactta ggcaattgaa cgaatgccat
    12481 cctgttttct atttgacgat tgaagaaaag ttagtcaaag tcataccaaa agatctgcca
    12541 aagccgcagc agcaggcaag ttaccagcaa ttatgtgtat tttcagatcg ttacgggaat
    12601 gaagatgcgc tgcttagttc cgtgttaaaa gaagaattca cgttgcaatc tgcaattgtt
    12661 tatcctgtct atgatacata catcgaagac ccgaagcgct ggtttcaact gactgtagag
    12721 gcgtgtacag gaaaaacgat gtatgaaggt acagaagatg aaaaacaaga ttttttaaaa
    12781 aaaagagaaa aaattttgca gcaaatgatc ttcttccatt agattttatc agtgaaaaag
    12841 ataaaaatgt tatgaaaaac aaggattcta tccattcaat atgtttacaa tttgaaaggg
    12901 ttagtgtact atttaaaggt atgcatgaaa cgtttttgat gtataaaatg aatttctaaa
    12961 ctcggaggga attaaatgac tgcaacattt gaaaaaaaag gcacgaatga tggtgtttta
    13021 aaatttacga ttgcgcaaga cgaaatccaa aaaggattaa caactgcgtt taataaagtg
    13081 aaaaaaagct tgaacgtacc tggattccgt aaagggaaag tttctcgtca agtattcaac
    13141 cgtatgtacg gtgaagaagc tttatatgaa gatgcattga atgctgtatt accagaagct
    13201 tatgaagctg ctgtcaaaga agctggcatc gatcctgttt ctcaaccaaa aatcgatgtt
    13261 gaaagcatgg aaaaaggtca agactgggta atcactgctg aagtaacagt gaaaccagaa
    13321 gtaaaattag gacaatataa agatctagtt gttgaaaaac aagaccgtga agtaacagat
    13381 gctgacgtgg atgcacgtat caaacgtgaa caagaagcac aagctgaatt agttatcaaa
    13441 gaagatgctg cagctgaaaa tggcgataca gtagtaatcg acttcgaagg ctttgtagat
    13501 ggcgaagcgt ttgatggcgg aaaaggcgaa aattactcac ttgaattagg ttcaggctca
    13561 ttcattccag gttttgaaga tcaattagtt ggtcataaat caggtgaaag tctagatgta
    13621 aatgtaactt tcccagaaga ttatcaagca gaagatcttg ctggaaaaga agcagtcttc
    13681 aaagtgacaa tccatgaagt aaaagcaaaa gaagtgcctg aattagatga cgaattcgca
    13741 aaagatgttg atgacgaagt atcaacatta gacgaattga aagaaaaata tcgcaaagaa
    13801 ttagtagaaa caaaagaaaa agcagcggac gatgcaaaag atgaagcagc gatccgtatg
    13861 gctgttgaaa acgcagaaat cgttgaattg ccacacgtga tggttcatga tgaagtacat
    13921 cgttcaatgg acgaattctt gaacaacatg caacgtcaag gtatctctcc tgaaatgtac
    13981 tatcaattaa ctggttctac agaagaagac ttgcataaac aattcgaagg tgaagcagaa
    14041 atgcgcacaa gaacaaatct tgtgatcgaa gcaattgcag ctgctgaaaa tcttcaagca
    14101 acagaagaag aaatcaatgc agaaatccaa gaattatcag aaacatacaa catgccaatc
    14161 gaacaaatca aacgcgtgtt gacagaagat atgctggaac atgatgtgac aatgaaaaaa
    14221 gcaatcgaat tgatcacagg tacagcgaca gaagcgaaag aatagaaatc aagtaataaa
    14281 tcatttctga agaagctact tctagaaaat tgtttattcg aaataaggga ctgtgacacg
    14341 attttgtcac agttccttat tttttgttaa gagtaaaaaa tgcaaaggta taggtcagtt
    14401 gatcctttgt taaacacttt ttctttttac taaactatgt tatgatagta aaagactgta
    14461 aagtctttga tagtttcttg catacttttt gcaagttaag gggtgaagac catgtatgac
    14521 aatccaagta gcaatgagac cgtacgctgt tctttttgcg ggaaaaccca agaagaagtg
    14581 aaaaagatcg tagctggtcc aggggtctac atctgtaatg aatgtatcga cctatgtaaa
    14641 gaaatcattg atgaagaatt ttatgaagaa gctgtacgcg aattcactga agtccctaaa
    14701 ccactagaaa ttcttgaagt attgaataac tacgtgattg gacaagatcg cgcaaaacgc
    14761 tcgttagctg ttgccgtata caatcactat aaacgtgtca atcagcaagc acaagaaaac
    14821 acagatgatg ttgaactgca aaaaagcaat atctgcttga tcggacctac agggtctggt
    14881 aaaacatttt tagcacaaac attagcaaaa acattgaatg taccttttgc aatcgcagat
    14941 gcgacaagtt tgacagaagc tggttatgtg ggggaagatg tagaaaacat tttgcttaaa
    15001 ttgcttcaat ctgctgatta caatgtggaa cttgcagaaa aagggatcat ctatattgat
    15061 gagatcgata aaattgcgcg taaaagcgaa aatgtatcta ttactcgtga cgtttccggc
    15121 gaaggtgtcc aacaagcctt gctgaaaatt ctagaaggaa ctgttgcaag tgtgccacct
    15181 cagggcggac gtaaacatcc acatcaagaa tttattcaga ttgatacgac gaacatattg
    15241 ttcatcgtag gtggtgcatt tgatggcatc gaaacgattg tgaagaatcg tatgggcgaa
    15301 aagacgattg gatttggtac acaaaaccaa aaactttctg aagacgaaag tgtcatgcag
    15361 catatcattc ctgaagattt actaaaattc ggattgatcc ctgagtttat cggacgatta
    15421 cctgttatgg cagctttaga gaaattgaca acagatgatc tggtacggat cttgactgag
    15481 ccgaaaaatg cgctagtcaa acaatatcaa aaacttcttt ctctagataa taccgaactt
    15541 gaattcgaac ctgaagcgtt gagagccatt gcgaaaaaag caatcgaacg caataccggt
    15601 gctcgaggac ttcgttccat cattgaagaa atcatgatgg atgtgatgtt tgatatccct
    15661 tcaaatgaaa acatcgaaaa agtgattatc acaaaagaag cagctgaatt gtctggtgag
    15721 ccaacagtaa tctataaaga ttcagagaaa aaagcgggtt gatgaaaaga ggctgccgta
    15781 aaagtcattt tggcttttgc gacagccgct tgctttattc atcaccttgt taaggagaga
    15841 gaccatgaaa gtccatcaag ctgaaattat cataagtgca gtggcaccaa aacaatatcc
    15901 agatacacaa ttgccagaaa tagctctggc aggacgatca aatgtaggga aatcttcttt
    15961 tatcaacaca ttgattgatc gaaagaattt agcccgtact tccggtaagc caggaaagac
    16021 ccagacattg aatttttatt tgatcgagaa cgcattacat tttgtcgatg ttccgggata
    16081 tgggtatgca aaagtatcca aaactgagcg agcaaaatgg ggaaagatga tcgagacata
    16141 tttaacgact agagaccagc tacgtgcggt cgtttcttta gtagatttgc gtcataagcc
    16201 ttctcaagaa gatatccaga tgtatcaatt tttgaagtat tatgatatcc ctgtaattgt
    16261 tgttgccact aaggcagata agatccctcg tggtaaatgg aacaagcatg agagcgaaat
    16321 caaaaaagcg ttggattttg atcgcaatga cacgtttatt ctcttttctt ccgttacaaa
    16381 acagggaaaa gaagaagcgt gggcagcaat cgaacaagca attgaatcat aaaaagaata
    16441 aaaaagactt accagtcaaa ctctttcata aaagtttggc tggtaagtc
//
LOCUS       DACEXQ010000031        16459 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.031, whole genome shotgun sequence.
ACCESSION   DACEXQ010000031 DACEXQ010000000
VERSION     DACEXQ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.031"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..192)
                     /locus_tag="IXH04_000828"
     CDS             complement(<1..192)
                     /locus_tag="IXH04_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAQ1851457.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGK"
     gene            complement(586..2313)
                     /locus_tag="IXH04_000829"
     CDS             complement(586..2313)
                     /locus_tag="IXH04_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1851458.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2306..3499)
                     /locus_tag="IXH04_000830"
     CDS             complement(2306..3499)
                     /locus_tag="IXH04_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1851459.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3686..4330)
                     /locus_tag="IXH04_000831"
     CDS             complement(3686..4330)
                     /locus_tag="IXH04_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1851460.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4424..4558)
                     /locus_tag="IXH04_000832"
     CDS             complement(4424..4558)
                     /locus_tag="IXH04_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAQ1851461.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4560..6692)
                     /gene="feoB"
                     /locus_tag="IXH04_000833"
     CDS             complement(4560..6692)
                     /gene="feoB"
                     /locus_tag="IXH04_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAQ1851462.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6689..7162)
                     /locus_tag="IXH04_000834"
     CDS             complement(6689..7162)
                     /locus_tag="IXH04_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAQ1851463.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7563..7787
                     /gene="nrdH"
                     /locus_tag="IXH04_000835"
     CDS             7563..7787
                     /gene="nrdH"
                     /locus_tag="IXH04_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAQ1851464.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            7946..10105
                     /gene="nrdE"
                     /locus_tag="IXH04_000836"
     CDS             7946..10105
                     /gene="nrdE"
                     /locus_tag="IXH04_000836"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAQ1851465.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10155..11120
                     /gene="nrdF"
                     /locus_tag="IXH04_000837"
     CDS             10155..11120
                     /gene="nrdF"
                     /locus_tag="IXH04_000837"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAQ1851466.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11209..12348)
                     /locus_tag="IXH04_000838"
     CDS             complement(11209..12348)
                     /locus_tag="IXH04_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1851467.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12657..13370)
                     /locus_tag="IXH04_000839"
     CDS             complement(12657..13370)
                     /locus_tag="IXH04_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAQ1851468.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13624..14325)
                     /gene="nagB"
                     /locus_tag="IXH04_000840"
     CDS             complement(13624..14325)
                     /gene="nagB"
                     /locus_tag="IXH04_000840"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAQ1851469.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14559..16091
                     /locus_tag="IXH04_000841"
     CDS             14559..16091
                     /locus_tag="IXH04_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1851470.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDNLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16282..>16459)
                     /locus_tag="IXH04_000842"
     CDS             complement(16282..>16459)
                     /locus_tag="IXH04_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1851471.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5105 a         3404 c         2859 g         5091 t
ORIGIN      
        1 cttccctgga attgctccac ctaaaaaacc acctatagac attcctgcaa tacaagtagt
       61 tgccttggcc caatcgaccg tacatttatt tttagtgcaa tacactccat ttccataata
      121 ttttccacta tgagtggtac ccccatagat aagttgtgtc tctttaatag acaaaatttt
      181 taaatgtttc ataatctaat cacccctaaa ttaatgtttt tagactatta ttctaacatt
      241 cattaaattt caaaaaacaa ttccttcttg aaacgtagca aaataattcc tgaataagaa
      301 tttaatcact tttaagtata taaactatgt atattcttta atattttttg agaatgcact
      361 tcgtacataa taaactttaa tcacattagt ctttcatacc tacgatcgat ctctttgact
      421 cgcacccata aattcataat agtttgtcgg cgtctttgaa cgtttcgaag atgagctacg
      481 cattctcttt ttttatagac aaaaaagata gagacgaaca tggagcttcc aaatttggat
      541 atttgatcca attttggaag ctccatttat ttcgtgtctg tctttttatt ttttccagct
      601 ttcgactaat tcttggttct ctttgatcca gtcttttgct gcttgctgtg gatcttttcc
      661 attgttgatt tccaacataa ctttttccat atctttttct gaccaatgga aattgtctaa
      721 tactttgtaa gcttctggct ggtcttcttt caatcctttt cgaaccatcg tatggatctg
      781 ctcttcgctt cccattccgt tttcaggatc ttctaagtat ttcagatcat atttggcaaa
      841 catccaatga ggtgtccatc ctgtaactac aattggttcg tgttttttga tcgcttgacc
      901 tagtgcaacg gtcattgctc ctgaagaaga tgtctctact ttccagtctt tcaagttgtc
      961 atatttttgg atcgtgtttt cagcagccgt aactacacca gctcctggct cgattcctat
     1021 gattttctta cctgcctgat cggttaactc atcgatcgaa tttacatcca tgtaggctgg
     1081 cactgctaaa ccaacttttg ctcctgtcaa attggcacct aggtcttcga cttgatcttt
     1141 atattgtgca tattgtgaac catgagtttt aggtagccaa gctgaaacca tggcatctga
     1201 ctcgccattt gaaacagatt tccacatgat tgaattatct aatggcgtca tcgttacatc
     1261 atagcccatt tgtttcaata cttcaccgac tacatttgtt gaagcaactt ctgtatccca
     1321 ttcaacataa gataagttga tacgttttgt ttctttggca gatgaaaaac tccctatacc
     1381 aatcgcacct aagatcacta ctactacagc accaatgatc agtccttgac gttttttact
     1441 tttctgtgtt gttgctcctg cagctttttt attgtttggc tggttcaaat gctgtgtaaa
     1501 acggtcaatg atgattgcta agataactaa tgcaaccccg ttgacaaatc cattcccgac
     1561 ttgtgctctt tgcaatgctg ataatacgcc gcgtcctaag ccgggtgcac cgatcataga
     1621 ggcaataacg accattgaaa gtgacagcat tgttgtttga ttgatccctg ccatgatcgt
     1681 acttttagct aaaggcaatt ctagtttgaa tagtttttga cgacttgtgc tcccgaatga
     1741 atcagaagct tctactaatt ctttaggaac ttgtcgaatc cctaaattcg taaaacgaac
     1801 ggttggtggc agagcaaaga tgactgaggc aaatacccct ggaaccatcc cgataccgaa
     1861 aaatgcaact gctggaatca aataaacgaa tcctggcatt gtctgcataa agtcaaggat
     1921 cggtttgata atactttggg ctttactgct ttttgccatc aagatcccta atggtacccc
     1981 aatgatgatc gagatcacac tagataaaag aacaagtgtg acagtattca tcaaatcatt
     2041 ccatagacct tggttataaa taaaccatag accgattaat gtgaacaacg taagtcctgg
     2101 acgtttctta gaaataaaga aagctgctac cgtcaataac aagataaaca aaggagctgg
     2161 aatcactaac agtaaatttg taatccccga catcactgcc tgtccaatcg tttgtaaaaa
     2221 tgaaaatagg ccagaaaaag ttgtcgtaaa ccattctgtg atattttcta cccagtttgc
     2281 cactggtaat tgatagttca atagattatt catgttcact cacctcgctt gtttctgcta
     2341 atgcttcaat cacactacct cggatgacga caccttttaa ttttccatcc tctacaactg
     2401 caagtggagt cggtgagtca taaataatat tgaaaatatc acttaccacc atgtctttcc
     2461 caatcaccgt cacattttca tctaggaagt cgatcaatgg acgtttttgc ttcctagctt
     2521 ctaacgcttg atctgcagtg atgatccctt tcaggtgtct ctttctgtca acagccatca
     2581 gcatgctgac ttcttcatta cgcatccttg tcaatgcaac gtttggtcca tcagattcga
     2641 tattcgtcgt caatgcaggt accatgatgt tttgagccgt aagcactttg gaacgatcga
     2701 cttcttccac aaattcacgt acataatcat ttgctggatt ggtcaatatt tcttcacctg
     2761 ttccgatctg catgatttcg ccgtctttca tcaaggcgat tctgtcccca atccgtaaag
     2821 cttcattcaa atcatgggta atgaaaataa tcgttttttg aacattggct tgcaggtcca
     2881 gtaattcgtc ttgcatttct cgccggatca gcggatcaag agccgagaac gcttcgtcca
     2941 tcagcaagat ctcaggatca tttgctaaag ctctggctag gccaacccgt tgttgcatcc
     3001 caccagataa ctgactagga tattggtctt taaatgaaag caaactagaa ttttctaatg
     3061 ctttttctgc tttttcttgt cgttcttctt taggtacacc acggacttct aacccgtatt
     3121 ctgtattttc caagatcgtt cgatgtggaa acagaccgaa gttttggaaa accatattga
     3181 ttttatgacg acggacttct cgcaattctt ctttgcttaa cttcgataca tcttctccat
     3241 caatatagat actgcccgaa gttggctcaa tcagacgatt caatagacgg atcaatgtgg
     3301 atttcccact cccggaaagc cccatgatca cgaagatttc tccttctttt acgtcaaaat
     3361 tcacgtcata cacaccaacc gttgcaccgg ttttctttaa aatctccgtt ttcggctggt
     3421 gatcattcat catatctaaa gcggcttgtg tctttttacc aaatatcttt gttaaatgtt
     3481 ttacttctac ttttaccaaa caaattctcc ttttttatgt aaattcattt ttctttctga
     3541 aaaaagtgac cacactatgt taacatcatg tagtcacttt gtcaaaaatg aaagaaattt
     3601 gcttgtccaa actgaaaaaa acttggctaa atctttgtaa ataaaaggtt tagccaagtt
     3661 aatatttttt taatatttca cacaatcatt ctgcaagctg atgctccggg taaaaaaagt
     3721 ttgtcatacg ctgtagtgta tattcatttc caataaaatg aacgatgtcc cctgccgtaa
     3781 atttagcata ggggccaggt gaaacaagca gttcttcatt atgagaaatt gcgataactg
     3841 tggcgccagt cgcctgccag atattcaact cgctaattga acggtccaga tgcattgctt
     3901 tttctgataa agataattca aacggaagta aaggattcat cttatgtatt cgcttcgttt
     3961 gattgaccag ctgatccaat aattgagaaa aatgattcag ctcatcttgt tgcttctcca
     4021 cacttgccat gatttctgct tttgtatctt ggatggattg tacatcttga tattgcaata
     4081 ggaaatcttt tgctttttcc ttcgaagcaa caaatgcccc acttccatgc cgtacttcca
     4141 tgattccaag atctaccagc acattgatag ccttgcgggt agtctcagga gaagcattgt
     4201 acgtactggc aagtgttgag cgggcatgga tcttctcacc cactttatat cggttttcaa
     4261 cgatacgttc ggctaaatca acagcaatct gctggtagcg cgggcggacg atttttttct
     4321 ttccagacat tttctttcct ctttcttttt ttactttccc tcatcttact atgctctagc
     4381 aaaataagca aaaaagggga gaatagcctc ttgcaacgac tgatcatcgc ttgatctgtt
     4441 ccttttttac tgggcaagct gtttgacagt catcacaatt cttaccggat tttaaacgag
     4501 tatagacaat tgcgcctgca cttccaaaga ttaacacact taataagaat gtcgccatcc
     4561 tattcctctc cttctaatga caatatagta taaaactggt tcttggattt tggctgacgg
     4621 aacaaaacat aaacgagtcc tatcatcagt aaaatagcaa gatacgtccc taacgctacc
     4681 tgtccaccct ccaataagtg tcccaactga taaatcacaa aactaactaa ataagctaaa
     4741 ccgcattgat agcctatggc gatccaagtc catttcttgg cattcatttc ccgatggata
     4801 gccccaattg ccgcaaaaca aggcgcacaa agtaaattga aggctaacaa tgaataagct
     4861 gctgcgggag tatatgtgtg ttgtagcact tgccagattt ctctgccatt ttcggatact
     4921 tccgcatgac caaacaaaat accaaatgtg ccaataatat tttctttagc gaccaatcct
     4981 gtaatcgctg cgactgttcc ttgccagttt ccccaaccaa gcggctgaaa aacaactgcc
     5041 aggattcgcc cgaaaaaggc taagatactt tggtcttcag aaacaggttg agcataaaaa
     5101 ttataagaag agctgaacca gatcacgata ctagaaacaa agataatcgt tcctgctttt
     5161 ttaacaaaag acttgctgcg gtcataggtt tgtcttagga ccgctgacca ttgaggaaaa
     5221 tgatatgcgg gcaattccat gataaaagga gctgtatctc cagcaaacaa acgtgttttt
     5281 ttcaagctga ttccagagaa aataattgca gtcataccaa gaaaataggc agaaggagct
     5341 acccaagatt gatgaggaaa aaaggcccct gctactaagg caataatcgg caactttgca
     5401 gaacaaggca tgaaggttgt gaccatgatc gtcaaacgac gatcttgctc gttttcaatc
     5461 gtgcgactgg ccatgacgcc tggcactcca catcctgtag caatcagcat aggaatgaag
     5521 gattttcctg aaagaccgaa tttcctgaaa agtcgatcca tgacaaaagc gatcctcgcc
     5581 atatatccgc agtcttccag aaagctcaag cataaaaaca atataatcaa ttgcggtaga
     5641 aatcctaaca ctgctcccac gcctgcgatg atgccgttca agatcagctc ctgcatccat
     5701 cccgctactt gccattcagc taacaatctg ccaacatgtt ctggtaacca tgtaccaaac
     5761 aatgtgtcat tgatccaatc tgtccccatt gttcctactg tttgaatagc cagataatag
     5821 atcagccaca tcacaaatgc gaagatcggc aaggctaacc aacgattcgt tgcaatctga
     5881 tcgattttgt cactcatcga taattggaaa gaatcatttg acacaacaca gagtgtacat
     5941 agttgggtaa taaattcata acgttcattg accagaatcg tctcactatc gtcgtccaat
     6001 agtttctctg ttaactggat cagttgctcg atctcttttt gctgagaaac tgctatctcg
     6061 taatcctctc ttaccttctg atctcgttca aaatatttca ttgcatacca gcgtttcttg
     6121 ccgctttgtt ctcctagtac atcttcaatt tcatgtaaag ctgcttctaa acgtggatca
     6181 tatgtacagg gaacaggaat agaaggccgc ttttttgcag actcctgaat cttttcgatt
     6241 agtttttcga ttccttgctt tttcaatacg ctgattggta aaactggtac accaagacca
     6301 taggaaagct tttctagatt gatttttttt ccttcttttt tgatgacatc catcatattc
     6361 agaccaatga tcataggaat gtctaaatcc aataactgcg tagttaaata aagatttcgt
     6421 tctaaattgg tagcatcaat aatgttgatg atcagttcag gctgttcctg aaccaaataa
     6481 tctcttgcaa tcacttcttc tggtgtataa ggagacaaag aatagatgcc gggtaggtcc
     6541 tgaatatgaa tgttcggatt ttttttcaac gatccttctt ttttttctac tgttacgccc
     6601 ggccaattcc ctacatattg attcgaacca gtcaagagat tgaacaagct agttttcccg
     6661 ctgtttggat ttccgactaa cgcgatattc attctttttc ctccctttgt atcaagatta
     6721 gttcggcctc tgactttcgt aacgtcagtt cataccctcg cactgtcagt tcgattggat
     6781 ctcccagagg ggctagtttt cggataacta tttttacatt tcgcgtcaat cccatatcca
     6841 ttaaccgtcg cttcactgcg ccttgtccgt ggattgctac gacgatccca gtctctcctg
     6901 cttttaattg atcaagagaa gtccagcttt cagaggaagc agtcttttta ttgacaaaga
     6961 tttgacctag tatttcccga cttactgcca ctcgatgact ttttaccagt aaaatggcat
     7021 tgttgcctga taaattgacg acgacgattt tacttccggc aacgatcccg atattttgca
     7081 aatggcgttt tgttttttct tctgtttcga ttcgttcgac ttgataaacg aaattctgct
     7141 ttgcctgcgc taaggtctgc atattgattc ctctcttgtg aaataaatca tgtattaaaa
     7201 tcattcatta tatgttctaa ttttcttacg tgttacgcaa ataaatgaga atcattctca
     7261 catagaatac tctcggtaat tgagaatgtc aattatactt gtgatattat gagcgaatct
     7321 ttagtattga tagataaaaa tgagaaaaaa atatttcttc atagttttcc acaatttttt
     7381 ctctattaaa tttgctattt tcccacacga tacttgtttt cagctctttt ttcaaaaaca
     7441 caaaacttag tattccacag tttccacaac acaatatgta gtgtttgttg tttgactttt
     7501 ttactaagag ttttatacta tctattatag ataataagaa tgtgttagag gaggcactca
     7561 ccatgaacgt aaaaatcttt tctaaaaata attgtatgca atgtaagatg gtcaaacggt
     7621 ttttaacaga aaatcaaatt gcttttgaag aagtcaatat cgatgaacag cctgaagcag
     7681 tagactggtt gaaagcacaa ggattccaaa gtgttcctgt cattacttct gatgcagaaa
     7741 cagttatcgg attccgtcct gatcagttgc gtaaacttgc cagctaacat gactaaaaga
     7801 atcaaaacgt gatacatgga tttgaagggc ttgagacagc accagttttt gtcgcaaact
     7861 tccaaatcct tttttaaagc ttaaaaacaa tgcaatgatt tgcaatcaag catcatcaaa
     7921 tacgaaatga aggttgggcg tacgtatgag tcttaaaaat ttacaagatg tcagttattt
     7981 caagctgaat aatgaaatca atcggccagt caatggtcaa atccctttga ataaggataa
     8041 agaagcttta gttgcctttt ttgaggaaaa tgtcaaacca aatacgatga cttttccatc
     8101 tattatggaa aaaatcaatt acttgatcga tcaagaatat atagaagcag aattcattca
     8161 attgtattca ccatcattta ttgaaaagct atatcaattt ttggacgagc aacatttttc
     8221 tttcaaatct tttatggctg cttacaaatt ttattctcaa tatgcgttga aaaccaacga
     8281 cggttccgct tatctggaaa cttatgaaga tcgagtagcc ttcaatgcgc tttactttgc
     8341 gaacggggat gaagaattag cacttgctct tgcagacgaa atgatccatc aacgatatca
     8401 gccagctaca ccttctttct tgaatgctgg acgtaaacgt cggggcgaat tggtgtcttg
     8461 tttcttgact caagtcactg acgatatgaa ctctatcggg cgctcaatca actctgcttt
     8521 gcaattgtcc cgtattggtg gcggcgtagg gattacttta tcaaatcttc gtgaagccgg
     8581 cgctcctatc aaaggatatg agggtgctgc aagtggtgtc gttccagtca tgaaattatt
     8641 cgaagatagt ttcagctatt ctaatcaatt aggtcagcgt caaggagctg gcgtggttta
     8701 tttgaacgtt ttccatccag acattattgc tttcctttca gcaaaaaaag aaaatgcaga
     8761 tgaaaagatt cgcgtaaaaa ctctttctct tggcgtgatc gtacctgata aattttatga
     8821 attgacacgt aaaaacgaag atatgtattt atttagtcca tatagtgtcg aaaaagaata
     8881 tggtgtccca ttctcttatg taaatattac agctgagtat gatcatctag ttgcaaatcc
     8941 aaacattcgc aaacaaaaaa tcaaagcgcg tgatcttgaa aatgaaatct caaaattaca
     9001 acaagaatct ggctatcctt acgtaatcaa tattgataca gcgaaccgcg agaatccaat
     9061 cgatggcaag atcatcatga gtaatttatg ttcagagatt ttacaagttc aaacgccttc
     9121 tgtgattaac ggcaaacagg aatatgaagt attaggaaca gatatctcat gtaacttagg
     9181 ctcgacaaat atcgtgaatt tgatgcaaag ccctaacttt ggtcaatctg tgcgtacgat
     9241 gactcgtgca ttgacattta ttacagatgc atctgatatc gatgtcgtac caccgatcca
     9301 aaacggaaat aagctgaatc atacgattgg attaggtgcc atgggcttac acacttatct
     9361 tgcaaaagaa caaatggaat atggctcaga ggattcatta gactttacaa atatttactt
     9421 catgctgttg aattattgga cacttgttga aagcaacaat atcgctcgtg aaagaaaaca
     9481 agttttccat aattttgaaa aatcagctta tgctgacggc acttatttcg aaaaatacgt
     9541 aactggagaa ttccagccaa aatcagaaaa agtcaaagca ttgtttgaag gcatttttat
     9601 cccaactgct gaagattgga atgctttgaa acaagcagta atgaaagatg gactgtatca
     9661 tcaaaatcgc ttggcagtag caccaaatgg ctcaatttca tatatcaacg atacgagtgc
     9721 aagtattcat cctatcactc gcatgatcga agaacgccaa gagaagaaaa tcggtaaaat
     9781 ttattaccca gcgccttatc tatccaatga gacgattccg tattacactt ctgcttatga
     9841 tatggacatg cgtaaagtaa tcgatgttta tgcaacggca caacaacacg tcgatcaagg
     9901 catgagtttg actttattca tgcgttctga aatcccagaa ggcatttatg aatggaagac
     9961 aacaacgaag caaacgacac gtgatttgaa tattttaaga cattacgctt tccacaaagg
    10021 aatcaaatcc atttattacg tacgtacctt tactgatgat gcagaagaaa tcggaagcaa
    10081 tcaatgtgaa agctgcgtga tctaatatca tagatagatt cgcttattca tattttaatt
    10141 aaaggagcat tctcatgtca gaaacatatt atgaagcaat aaactggaat gaaattgaag
    10201 atatcattga taaatccact tgggagaagc tgacagagca attttggcta gatacacgta
    10261 tcccactttc aaatgaccta gatgattggc gtacattgtc tcaattggaa aaagatacag
    10321 taggacatgt atttggcggt ttgacactgc tagatactgt ccaatctgaa agtggcatgg
    10381 atcagttgcg taaagatgtc cgcactcccc atgaagaagc cgttttgaac aatattcaat
    10441 tcatggaatc cgttcatgcc aaaagttatt cttctatttt tagtacactg aatacgaaaa
    10501 aagaaatcga agaaattttt gactggacaa atacgaatcc ttatctgcaa aaaaaagctg
    10561 agcgtatcaa tgaaatctac aaacacggta caccgctaga aaagaaaatt gccagcgtct
    10621 ttttagaaac attcttgttc tattcaggtt tttatactcc tctttattat ttaggaaaca
    10681 acaaattggc taacgtagca gagatcatca aactaatcat ccgagatgaa tctgtacatg
    10741 gaacctacat cggttacaaa ttccaattag gattcaacga gttacctgaa gcagaacaag
    10801 aaacgttaaa agactggatg tacaatcttc tttatgagct ttacgaaaac gaagaacgtt
    10861 atacagaaga attatacgat ccaatcggct ggacagaaga agtcaagaca ttcctacgct
    10921 ataatgccaa caaagcattg atgaacctag ggatggatcc actctttcca gatactgcca
    10981 atgatgtcaa cccaatcgta atgaacggga tttcaacagg cacaagcaac catgacttct
    11041 tctcacaagt cggaaacggc tatttgttag gtcatgtaga agcgatgaaa gacgacgatt
    11101 atttgattgg attggaataa ggatttattt taattcaaaa atttttcgaa aaataaaaag
    11161 agtttagcgt acagatattt taaatgtaca ctgaactctt ttttatgctt attcacttgt
    11221 ttcttccagc acttttggac tgtctaactt ttccgaaaca tgttccattc ttctcaataa
    11281 atagattttc cggatattta atattatcgt cttaatcact gcatagacag gtatgcaaat
    11341 cagcatgcct aatattcctg cgacacttcc tgctccaatc aataccaaga tgatcgtcaa
    11401 aggatggata ttcattgatt tcccaaataa caacggcttg atcaaatttc catctaattg
    11461 ttggatcact aaaatcgaca acgccatata caaagcttga aatggagaga taaacaaacc
    11521 gacaatgatt gctggtaccg ctccaataaa agggccgaca tacgggataa tattcgtgat
    11581 tccgcaaatg accgcaagca acaagctata aggctgatcg aatattctca ttgccaaaaa
    11641 gctcattaca ccaataatca aagcatctaa gatcgtactg ctgatatagg cagataatgt
    11701 ttcgtttaat tcttttaccg tttggcgtaa ctctttacga atcgctttcg gaaagaagtg
    11761 agacaacgca tcaataaatt tgtgcccatc cttgaacata aatagcaaaa taaatggaac
    11821 ggtaaataac aaaataaaaa attgcatcat aaatgaaatg atttttgaga cacttgcaga
    11881 taaactgact aatatgatat tgaacaaatt actaagagat aaatttgctg atgctaattg
    11941 ttcttccaaa ttgaaatttt taaactcttc ccgctgcgcc aattcattta accatttgct
    12001 tgtttcatct gcatacattg gcaagctctg ggtcaattga attgtctgtt ccattaactg
    12061 aggcactaca ttcatgacag ttaaaacaac taaaatgatt acgaccaaaa acgtcaataa
    12121 aaaaccgaac actctcggga tccgatgctt ttcgagaaaa acaacgattg gatcgaacat
    12181 ataatagata aaaccagcaa caagcaatgg taaaaaaatt gccgaaagca ttttcaatat
    12241 tggactaaaa atattcggca tttgtaaaat aaagtacacg ccgatgatcg tgagtactag
    12301 ctccactgtc cagaaaaata attttgattg tttaaaacgt gattgcatac atctcctcca
    12361 aggtcctttg atacttctat tctatcattt aacaaagagt tacgcgctga gattttcatt
    12421 tgcttaaaaa aaacaaaaaa aataaacata ttttaatgag tagacgagta cttaaacaaa
    12481 aacacggttt ggagtagtga caaaaattat actgcagtcc ctttttccaa ataaacggtg
    12541 ttcaaaagct tgcatcttcg taataagccc agccaaacga aaaatataag gaactatttt
    12601 tatttggctg ggcttattac ttgatgtagg acatctattt tacaacctca gatcatttat
    12661 tgcgcaatac tgactaaatg caactttgtg atatttttgt gtacggacaa ctcctcgatc
    12721 acatcggcat aagtgagccc ttttggaaga tcaatcgtat agatattttt gtacactcgg
    12781 tagtcatcaa cgaattttac gtcaaaattc acatcttcga ttacgatatt tttctcttca
    12841 aaataatcgg taagaaaatc tttggtttct tttctatgga taaattggat ttctaacttt
    12901 ttagtcgtag gtacatggat cacgcgtttc actactgtga gtgcaatagc aatcccgata
    12961 aaactgatga tcgcaatgga atagtaaccc atgccgatgg cgatccctaa tccagcaatt
    13021 gcccataaag aagcggcagt cgtaagtccc gtcaccgatt gtttcgtgac gatgatcgtt
    13081 cccgcgccta agaaaccgac accactcacc acttgagcaa tcagccttgc ttcatctgaa
    13141 cgaatcgttc cagcaagctg tggatactct aaagcatctc ttaatgcacc actagcgatc
    13201 tcaacttgga tcaatgcaat gatacatgct cccatacaaa ctaaaatatg cgtacgcata
    13261 cctgctggac gatttttata ttgtcgttca aaaccgattg ctccgccaaa tagcatagcg
    13321 atccctaaac gtaaaataat ctctggaatt gtcaatacca tatcattcat ctaaacaccc
    13381 tccagtccaa tatatcatgt ctgaaaggag cagacaaaaa aagtgcaatt tttacctctt
    13441 acgaaaaaag gactgtgaca aaatttttgt catagtcctt tttatgaata aacggtgttc
    13501 aatcagctga ccctcggtat tactcaacaa acggcaaaat atggggagct atttttcctt
    13561 tgtcttctct aatactcagg tcaaaacgct gatttcacaa cctctttagc ctactattga
    13621 aaattacaac ttacttgctg cttcttcatc gatgatcaca acaacattgt cgtgattttg
    13681 caatacactt gctggcaaat gattcgttac aggtccttca atcatccctt tgattgcatc
    13741 agctttgctt tcgccaaatg ccatcaagac gatttcttta cttttcataa ttgaaccgat
    13801 tcccattgaa acagcttttg ttggtacatc ttctgcttta tcgaagaatc gtttgtttgc
    13861 attgattgtt gattctgtta agtcaactac tgaagtcgtt ccgtcaaatg aagcaccagg
    13921 ttcattgaaa ccgatatgtc cattttgccc gattcctaag atttggatat cgattggatg
    13981 tgcatcgatg atttgatcat actttttgca tgctgcttcc agatcttctg cttttccatc
    14041 tggtacaaac gtttctttga atggtttttt atcaaataat tggacatcca tgaaatgacg
    14101 gtagctttgt tcatctgaac cgcctagtcc aacgtactca tctaagttta cagaagtcat
    14161 ttcactgaaa tccaaatcgc ttgaagtcat ttctttatat aaagtaactg gtgtgcttcc
    14221 tgttgccaat cctaatactt ttgcaccttt gttcatacct tctttgatga tttcaaatgc
    14281 tttttttccg ccttcttcag cgttttttac acgaatgatt tccatagtga ttgctccttt
    14341 atattttggt atagcccaat tatagcattc tttttttctt tttacaataa ttagtataga
    14401 ccaaattgca tttttttcat tttcttgaca ataaatagga atccgtgtta ggtttcataa
    14461 gatggttcaa tcaaacaatc catgttgcat gaaaaccaag caagtgattc actgtggacg
    14521 ctttatgtct gcagtttttt tatgaaaaag gagttattat gagtatactg acattaaaca
    14581 acattaccca gcaatttggc gacaaaatcc tctatgaagg tgtcgatctg caattaaatg
    14641 ctggcgaaca tcttgggctg attggacaaa acggagccgg taaaagtaca ttgattaaaa
    14701 tcatcactgg tgagatctta ccagatgacg gacagatcca ttggcaaaaa aatattcaca
    14761 aaggttacct cgatcaatat gtggaagtcg atgaaacatt aacaatcgaa gaatttctaa
    14821 aaactgcgta cgcaaaagaa tacgaaaagg aagaaaaaat tgcttttctt taccaaacat
    14881 atagcgagac cttcgatgat tccttgctgg aaaaagctgg gaaactccaa acggaattag
    14941 accaaggagt gttttatcaa atcgacaact tagttgctga gatgagcagc gggttgggga
    15001 tcgatgtctt aggtttagat tcactgttga aaaatctaag tggtggacaa cgttcaaagg
    15061 tgattttagc aaaactactt ttagaaaacc cagatgttct gatcttagat gaaccaacca
    15121 atcacttaga tgatcagcat atccaatggc tgacgcagtt tcttcaggat tttgacggtg
    15181 cgtatatcgt gatttcccat gaccaagaat ttcttgatca gatcaccacc catatcgcag
    15241 acatcgagtt tggaaagatt actaagtata ctggccatct aaaacaagca ttgaaacaaa
    15301 aggaacaaaa tcgcgaaagc tatttgcgcc aatatcatgc tcaacaaaaa caaatcgaaa
    15361 agaccgaagc ttatattcga aaatacaaag ccggttcccg atcaacgatg gcaaaaagcc
    15421 gacaaaaaca attagataaa atcgaacggc tgacgcctcc agcttcagca agtaaagctg
    15481 tctttgattt tccattctcg cctattgtca cgacattagc agtggaaaca actgatttag
    15541 tcatcggata tgatcgaccg ctacttgcac ccatcaattt gaccatccgc tttggtgaaa
    15601 atattgccat tcgaggcttc aacgggattg gaaaatctac tttattaaaa acactgatcg
    15661 gtgagattga aaaaataagt ggcgatttcc acttcccaga taacacaaaa atcaattact
    15721 tttcacaaga tctgtactgg gaaaatccat tagaaacacc actacaatat ttaagcagtc
    15781 aatttccaaa agcgactatc aaagaattac gtcgccaact agctaaagca gggttagtta
    15841 atcaattagc aagtgaacct ctcactacat taagtggtgg ggaacaaact aaggtgaaac
    15901 ttgcacagtt gacaatgaac caaggtaatt tactgattct cgatgaaccg acgaaccata
    15961 tcgatcaaga aacgaaagaa agcttacaag acgggataca gaaatttcca ggaacagtaa
    16021 tcatcgtctc tcatgaacaa gaattttatc aagatcttgt gcaacgagtc attgagattg
    16081 aaccaaaata aacgtttgat tgaatagttg aaaaaaagga gctgtgacaa aagttttgtc
    16141 acaactcctt cttctgaata aatggtggcc aattagctga ccttcggtat tagctcaaaa
    16201 aacggcaaaa tatgaggagc tatttttatt tggctattct tattacttga tgcagaacag
    16261 cttttcacaa cctcttttcg tctatttttt attttcgaga tttctctttc ctttaaaaac
    16321 aactgtttga aagaagaacg cacatccttg aaaaacaagc ccgctaaccg ttacacctaa
    16381 ccagccatac tgctgccaca ttgtcgtccc tagcaatgaa ccagccgcac ctccaataaa
    16441 gtacataaac ataaataca
//
LOCUS       DACEXQ010000032        16433 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.032, whole genome shotgun sequence.
ACCESSION   DACEXQ010000032 DACEXQ010000000
VERSION     DACEXQ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.032"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(46..321)
                     /locus_tag="IXH04_000843"
     CDS             complement(46..321)
                     /locus_tag="IXH04_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAQ1851472.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(564..1874)
                     /locus_tag="IXH04_000844"
     CDS             complement(564..1874)
                     /locus_tag="IXH04_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAQ1851473.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(2056..3285)
                     /gene="rpsA"
                     /locus_tag="IXH04_000845"
     CDS             complement(2056..3285)
                     /gene="rpsA"
                     /locus_tag="IXH04_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAQ1851474.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(3420..4100)
                     /locus_tag="IXH04_000846"
     CDS             complement(3420..4100)
                     /locus_tag="IXH04_000846"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAQ1851475.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(4149..4784)
                     /locus_tag="IXH04_000847"
     CDS             complement(4149..4784)
                     /locus_tag="IXH04_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1851476.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(4850..6253)
                     /locus_tag="IXH04_000848"
     CDS             complement(4850..6253)
                     /locus_tag="IXH04_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAQ1851477.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(6240..7271)
                     /locus_tag="IXH04_000849"
     CDS             complement(6240..7271)
                     /locus_tag="IXH04_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851478.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            7302..7523
                     /locus_tag="IXH04_000850"
     CDS             7302..7523
                     /locus_tag="IXH04_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAQ1851479.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(7688..8425)
                     /locus_tag="IXH04_000851"
     CDS             complement(7688..8425)
                     /locus_tag="IXH04_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAQ1851480.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(8578..9918)
                     /locus_tag="IXH04_000852"
     CDS             complement(8578..9918)
                     /locus_tag="IXH04_000852"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAQ1851481.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(10155..11678)
                     /locus_tag="IXH04_000853"
     CDS             complement(10155..11678)
                     /locus_tag="IXH04_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAQ1851482.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(12056..12667)
                     /locus_tag="IXH04_000854"
     CDS             complement(12056..12667)
                     /locus_tag="IXH04_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1851483.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(12790..12915)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(12997..13713)
                     /locus_tag="IXH04_000855"
     CDS             complement(12997..13713)
                     /locus_tag="IXH04_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ1851484.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(13719..14288)
                     /gene="scpB"
                     /locus_tag="IXH04_000856"
     CDS             complement(13719..14288)
                     /gene="scpB"
                     /locus_tag="IXH04_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAQ1851485.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(14272..15069)
                     /locus_tag="IXH04_000857"
     CDS             complement(14272..15069)
                     /locus_tag="IXH04_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAQ1851486.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(15039..15410)
                     /locus_tag="IXH04_000858"
     CDS             complement(15039..15410)
                     /locus_tag="IXH04_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ1851487.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(15429..16316)
                     /gene="xerD"
                     /locus_tag="IXH04_000859"
     CDS             complement(15429..16316)
                     /gene="xerD"
                     /locus_tag="IXH04_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAQ1851488.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         4612 a         3564 c         2785 g         5472 t
ORIGIN      
        1 aaaagcactt gtagccctag agccacaagc gctttttcaa ctgtcttatt taacagcgtc
       61 ttttaatgct ttacctggtt tgaatgcagg tactttgcta gctggaattt cgatttcttc
      121 accagtttgt gggttacgac ctttgcgagc tgcgcgttcg cgaacttcaa agttaccaaa
      181 accgattaat tgaacttttt cgccgttagc taaagcatct tgaatagttg aaaatacagc
      241 atctactgct gcagttgcat cttttttagt taaatcagta gctgaagcaa ctttttcgat
      301 taattctgct ttgtttgcca tggatagatt cacctcctcc acaagagata tgatagaaat
      361 atcatttagt taaaactttc tgagtggtcc agaagtttaa aaagaaatat tgattaactt
      421 caatattctg ttatcacgat atcatatgaa ccgcttaata gcaaggtttt taccaatttt
      481 cacatgcttt ttttagtcat agaaacgtaa caaacgaaac tgtctttttc ttttgaaaaa
      541 aaaacgtcga tatggtaaaa tgcctatttt ctcctacgag ggataattcg gatggacgtc
      601 ccttcaaaag taaatgcttt tctgatttga ttttctaaga aacgttcata agaaaaatgc
      661 atcaattcct cttcattgac aaaaacgaca aatgtaggtg gtttgacagc gacttgtgta
      721 gcataaaaaa tcttcaggcg ttttccttta tctgttggtg ttggattgat tgctacagca
      781 tccataatga tatcattcaa cacagccgaa gagatacgaa gattctggtt catacttact
      841 tgttcgatca tttcaggcag acgttccaac cgttgtttag tcactgcaga aacaaaaacg
      901 attggagcgt aatcaagata ccggaattca tcacgaatct cttgttcgaa atcacgcatt
      961 gtattggttt ctttctttac aagatcccat ttattcacga cgataataat tccacgacca
     1021 gcttcatggg cgtaacccgc tactcgcttg tcttgttccc gaatcccttc ttcagcattc
     1081 agtaccatca aaacgatatc agaacggtca atggcacgca ttgcacgcat cacgctgtat
     1141 ttttcagtat tttcgtatac ttttccgcgt ttacgcatac cagctgtatc tatcattaag
     1201 aatttctgac cactttcaga aacgaagtgg gtgtcgattg cgtctcgggt cgttccttca
     1261 atatccgaaa caattacacg ttcttctcct aaaatcgcat tgatcaaaga ggatttacca
     1321 acatttgggc gaccaatcaa actaaactta atcgtgtcgt cgtcctcttc ttcagtctct
     1381 tcagagaaat acttcactgc ttcatctaaa atatcaccaa tccctagtcc atggcttcct
     1441 gaaacaggat aaggatcgcc tagccctaag gaataaaatt catagatatc gtttctcatt
     1501 tctggattat ctactttatt gactgctaaa atcactggtt tattacttct gtataaaatt
     1561 ttcgcaacca tctcatctgc gtctgtcacg ccttcgcgtc cgctgactac acaaatgatt
     1621 acatctgctt cttcaatcgc gatttctgct tgatgcttga tctgatccat aaaaggttca
     1681 tcgccaagat caattccgcc tgtgtcgatc acgctgaatt ctcgacctaa ccattcacct
     1741 tttgcataaa tacgatcacg agttactcca ggagtatctt cgacaatcga gatacgctca
     1801 ccagcgatgc ggttaaaaat cgtagactta ccgacgttcg ggcggccaac gattgcaatt
     1861 gttggatttg ccatattatt tcctcctcac aagtttccaa tctcacttag tttaacgaat
     1921 gcattgccaa ctttcaagca tagattgtca attaaacgaa gtatttttat tccttattga
     1981 aatactaatc gcttttcaaa agaaaaactg agacaaaagc agctgctttc atctcagtca
     2041 cagatacaaa atcaattatt tttcagaatc atcggttgct ttttcttcta cttcttcttt
     2101 caacgcatca cctaaaatgt ctcccatagt aaatccagta ttttcttctg gtaattcata
     2161 agactcaaca ttttttggtt cttcttctga agctggtttt tcttctaaag ctttgatact
     2221 caatgcaata cgatgttctt ctggatggat ctctaatact ttcactttca cttcgtcacc
     2281 ttcatgaagc acttcatgtg gtgtcgcaat atgtttatgt gaaatttgag agatatggac
     2341 tagcccttct actccaggga acacttcaac aaaggcaccg aagcttgtca aacgtttaac
     2401 tgttccgtct aatactgtgc caactggagc ttttgtatcg atttctgacc aaggtccagg
     2461 ctgtgtgtct ttgattgaca aggaaacacg ttcttgttca ggattgatag acaacacttt
     2521 gacatttaca tcatcattga cagccaatac atcacttggt tttgctacat gactatgtga
     2581 aatctctgag acatggacta gaccgtcaat tccgcctagg tcgacaaatg cgccaaaatc
     2641 agtaagacgt gcaacacgtc cactgagaac atctccctca tggatagttg aaagaatttc
     2701 ttttttcttt tcttctcttt cgttttccac taccgcttta tgcgataaga tcaaacgatt
     2761 ttctgatggt tcgatttcga tgattttaaa gctcaatgtt ttgcctttat agtctgaaaa
     2821 gtctgcaaca aaatgatctt ccaccattga tgcagggaca aaaccacgta caccgacgtc
     2881 aacgactaaa ccgcctttga caacatttgt aacaggtgct tcgatgacct tgccctcttg
     2941 gaaatctctt tcgatatctt cccaaacttt tttagcatct aaacggcgtt ttgaaagtag
     3001 gtagctgccg ttttctttat ctttgccgat cgtactgata acgactaaat ctaatacatc
     3061 accgactttt actacttcgc tgatatcttc taccggcaaa gttgataatt cttttgccgg
     3121 aacgactcct tcaactccag ccccttcgat tccgacaatc acttgtttgt cttcaatgac
     3181 taaaacttcg cccttaacaa catcgccaac ttttacttct tgtacgcttt tcattgcgtc
     3241 ttccattgac tggttgttct cttgattttg atcaaattct gtcataacac ttgtcctcct
     3301 aaccaacatt ctgccgtaag tacggtactg tttttagttt aattgattat tcaaaaaaaa
     3361 taaagcgatt tcgttcgttt tccttgaaaa aacaattgtt tttcattttt tttcttaatt
     3421 taaaaaagaa gataaccttt tttcaaaaca acagctttta ttgctgcaac gacttctgga
     3481 atcgatttgc cagtcgtatc gatacgaaca gcatcctctg cttgcttcag cggagagacg
     3541 tcccttgttg aatcataatg gtctctttcc tgtatctctt tcgtcagtgt ttcaagatca
     3601 gtcggaattc ctttttcttg gttctctttg aaacgtcttt ctgcacgctc acttacgctt
     3661 gctactaaaa agattttcac ttctgcattc ggtaatacag ctgtcccgat gtctcttccg
     3721 tccatgacaa cgccaccgcg ttcccctatt tttcgttgga cagcaactaa ttcttctctg
     3781 acttttccat gtgcagagac ttcagaaaca gcttttgtga catctggttg acggatttct
     3841 aaagtcacat cttttgatgc tatgaataca tgctgtccat cttcttgttg cgaaaaagag
     3901 ataggatact ttttgatcag cgaaactagc ccttcttcat ctgaaaaaga tacattgttc
     3961 gtaatagcta aataagttac tgcgcgatac atggcaccgg tatctgtata aatataattc
     4021 aattcttttg ctaatatctt ggcaacggta cttttcccag atgaagcagg cccgtcgatt
     4081 gcaatactga tttgtgtcat gatataactc ctttttctca gaaaaaaagc cgcaaagcgc
     4141 ggctattatt atttaattct taattcttga ccagggtaaa gcataaagtt gtttggatct
     4201 aagccattca attcaaacaa ttgatcagtg gtgatccctg cacgctcagc cacttgtttt
     4261 ggaccttcgc ctgcttgaac agtcgttgtt gaaccggctg cagctgatga agaaggtgtt
     4321 gtttctgctg cattgctgct tgaatcccct gcttgataag ttgcctcact tgaggaagtc
     4381 ggtgttgtct ctgctgcaat actgctttct gacgttacac ctgtatcttg agacacagta
     4441 gattcttccg cgattgtact agattctact gttgaagtct cagttgactc ctcttttgtt
     4501 gtacttgtag aactttctac tactgatgtc gaagtttcag aagaagttgc tgctgttgca
     4561 ttgttgcttg atccatttct gatccagaaa taagcacctg ctggaatagc gatacataag
     4621 accagtaaaa tcaaaaatag cgaaagaaat aatgtatttc ctttcttttg ttgtctctga
     4681 gaacttcttg atgaaacttc atcatctgta tcgtagattg gttgttccca tggttcttgt
     4741 gtttcgttat tttctgtaga ttgatgtcta tcttttctgc tcactgtttt acctcctaaa
     4801 ctctatcgtg tgtattatac cataggaaaa agtgtcttgt cctaaaaaat caatgttcca
     4861 tgtttttttt aaataaattt agtaaaatcg tttcccaatg aagttgttca gtcttagaaa
     4921 aaagcttcct ctttggcaga agttctgctt ttgctccatc aatatcacaa caattctgcg
     4981 gcttttcgga gagtttttcg ccaaaataag ctaagatgaa ttttctgcgg cagttttctt
     5041 cattaatata gcgaagcatc tgctgcaggc ggaattcttt ttctttttcc tgacgtttga
     5101 gccgttccac ccaattttct ggtttttcag actgctggat cagctccatc catttttttt
     5161 gcagctcatc aaagggagct tgctttgtgg catattccaa ataaagctca aaactttgtc
     5221 gctgttctct agaaagttga ttaaagaaat aatggatacg ttcatcccca ctttgataaa
     5281 aaagaattgc tgcactttct tcctgatctc tgcctgctcg accgatttct tgtacatagt
     5341 tttctagact gtctggcaaa tcataatgga ccacataacg gatatcagat ttatcgatcc
     5401 ccataccaaa tgcattcgta gcaatgagaa actgcagctg attttttaca aactgctgtt
     5461 gaagttgtct tctttgagct gcatcaagac caccgtgata gtatccaact gtaaaacgtt
     5521 ccctgaataa atgatacaat cgttcgactt ctttttttgt tgcacaataa atgattgccg
     5581 ctccttgtgc tctttccatg aagtgttcaa gttcttgttc tttttgctgc gttttccgaa
     5641 caaaaagcga gatattttgc cgattgactg attgtctgat ctcttttggt ggtcgatcaa
     5701 atagtacatg acgtatatct cttgctacta aatctgttgc tgtcgccgtt agtgccagtg
     5761 tgaccgggtt tcctaaaagc tttcggattt ttcctaactg ttgatattct ggacggaaat
     5821 caactcccca ttgagacacg caatgagcct catctactac atataacgca atattttgtt
     5881 ttttcagatg ctctaaaaga gaaggattag tcaacatttc gggactcaaa aacaaaaatt
     5941 tatattgcga tagatgacgg aggacgtatc ttctttcaga ttgcgaaagt aaactattga
     6001 aagcagctac tcgtttctcc cctcgttttt gtaattgcgt cacttgatct tccattagag
     6061 aaagcagcgg tgacacgatg atgaccagac cttccattaa ataccccgtc aattgatagc
     6121 aaaggctttt tcccgttcct gtaggcaaaa tagcgagagt atcttctcca tcaagcaacg
     6181 cctgaataat ttcttcttgg ccgggacgaa atgaatcaaa tccaaaatat ttttttaatt
     6241 catttattaa cattctgtat cgcttccttt ctcaatagat agatttgata caaacgaaat
     6301 tctccataat ctaattgtcc atctacattc cattcgctaa agcgccactc tcgcacggaa
     6361 tccttatttg gttccaaacg ttcgattgtt tctgcagaga gaatccactc gtatggaaaa
     6421 tcatcaaaaa acaaggccca ctcaataaga tgatctgtta ctgttcctct ttttaaatga
     6481 cggattgcca tgatctggtc aattgtctga ccattcataa acatctgctt ggtgatcatc
     6541 atgctttgat tgaaattttg ctgtaataat gaatcgatca aagcgtgcca aagcgaatct
     6601 ggccgttttt cgatttgtgc taacaaaagg tggatacatc tgctttgttg cagataaacc
     6661 tctgactcat cattgttttt tgccagctga taaggaagca gccctgttcg ctcatttcca
     6721 gataattggt tagctaaaaa atctgctgct tcttctggta tctccgaaaa cagctgcacc
     6781 aaactctcat acgcagtatc aatcaaaatt tcacgtggca attggctttc actaagccat
     6841 ttttttagtt ggaaagtata aaaaggactt gtctctgctg gaagatagtc ttgaataccc
     6901 gttgccagat tagaaattac ttgaacagca aatttaatga gccgccaact agtttcccct
     6961 gttctgccat agcggtcaaa acgcaaacca gtatgttcta tttgatgttc ggacaacata
     7021 tcagcccctg caggcgttag ctttgcttca ttttcattta tttcgatcca accttgctgc
     7081 gcaatttttt gcaaaatttg ataaaactta tcctgcttca attctggaaa actgttatgg
     7141 ataaacaaaa gttcgtgggt aaatccataa ataagtactg aagtcgttcg ttttcccaca
     7201 aggagttggt aaagagaact aatccgcagc ttgtgtcctg atgaaaatag cgataaaata
     7261 aaaacaatca tattattctc cttaagttag gatggttaga aatgtcacta ctatgtaaac
     7321 ttgtacctga acgctgtatt gcatgcggtc tttgtcaaat cgaagcgcca gatattttcg
     7381 attatgatga agaaggaatc gttttatttg cccaagaacc agaggctcat caacagttcg
     7441 tgatgccgga agaagaaaca tatgtaaaaa aagcgtatca aaaatgtcca gttcgagcga
     7501 ttttattaga aaataaaata taaatataaa cgaaacttta aaaacgtaca ctaagttctc
     7561 taaacttgaa aataagccgg gatctgtttc tcttgttccg tgaaatcccg acttattttg
     7621 aaagattcat ttgctatcta tagttaattg actagttttt tcacggaccc tataggaccc
     7681 atttatatta tttcaacttt ttcagccagt tttccaatgt attgacgatc acagtttgct
     7741 ggtctgcgtt agaaatttct gcctctccat ctcctttttg ctcaccatag ctgccaaaac
     7801 ctccatgatt tccgccattg attgatacat actctgttct ttccggtaaa tactgcttag
     7861 cttcttgata agcttcctga ttcaatactt tatcttttgt cgccgtgatc gataaaacag
     7921 gaacagctga ttctgtcaat gttccttttt tgtcgggata acttgccatg aagaataccc
     7981 cttttaaatg tttgtctgaa tgattcttat tgacataacg acttgccatt acgccaccta
     8041 atgaatggcc accgatcaca taatcggaat ttggaaaatc ttctttgacc ttatccgctt
     8101 tgtttccgcc catcactgct agatcaagtg gcattttgac aatagcgact gaaaagccgt
     8161 tttttgctaa atcttgcgcc caaatactat aactttctgg ctctacgaga gcaccaggat
     8221 aaaaaataag taatggagac tttccatgat tctttttctc tccttcaaaa aataagtaat
     8281 cttctttgtt ttttccttct tttaccaaca cttgcgcttc tgacgatggt tcgtacgtat
     8341 tttgctgtat atagtaataa ccggctccac cgagtaatcc taaaatgatg actaagccaa
     8401 ttagtattcg tctaaatatt ttcactttct cccacctttt ctatctgagt gacccctttt
     8461 tcttaatcaa gtatgattat accttaaaaa aataagagag aaatgcaaac tttccagcat
     8521 caaataaaaa aagacggaat atccatttga atatccgccc tttttgtatg tttattttta
     8581 tttagcacat aaacgataca cagcttctgc ataaatttcc attgctttat acatactact
     8641 taattcccat cgttcattcg cctggtgcat aaagtcagga gtatcaggga acatcgcacc
     8701 gaatgccaca cactggttca tcgtacgagc aaaagtagcg ccgcctgata caaacggttc
     8761 agtcatgtct ccagtaatat cgcgatatgt ccccaataga tttttcacta actcactgtc
     8821 tagtggcacg tataatgaat caaggaagtc aaattcttca taagtcaacc catatttttc
     8881 tgctgtttta gttaatttag ctactaaatc atctttcttg aatgtaacag ggattctcat
     8941 atctacaccg atttttgttt gttctggtgt gatttccaaa cttgcaaagt tcatcgttag
     9001 ctcgccggat tgttcatctt ctgttttgcc cacgacagct tcaccagttg cattttcttg
     9061 aactaatttt cctaagaaat tgattgggcc aaaatcaaat acttctgaaa gagcaattgc
     9121 cagacgagaa atagcattca ctccttgtgc tgcatctttc gcgtggatac ttttaccaag
     9181 aacgacgatg ccttcacctt gttcttcgaa atcgaattcg tgttttttca atgcttcttt
     9241 tacttcagaa agcttttctc cagagtaagg agctgcatca ggaacaacat tcaacgctcc
     9301 tccagctttt acagaaaact cgttcgttcc tggtcctgtc aagtaagctt gcaataagcc
     9361 tttttctgca taaattaaag gaaattcagc atctggcgca aatccttgcg taattccttc
     9421 ttctttttcg ttatattttt ctaaacaacg ccataaattt tcttcatctg taccaaagat
     9481 aaaacgaata cgtgtcttga attccacgcc agcatcaatc aaagctttga cggcatacat
     9541 tgccgcaata gaaggacctt tgtcatcttg ggatccgcga ccaactaacc agccgtcttt
     9601 tatagttgga tcgaatggtt ttgtttccca attattttcg tcccctgctg gaacaacgtc
     9661 catatgacac aagataccga ataattcatc tccagagcca atctctgaat aaccgtaata
     9721 gccttctgga tctttaaatg ttctaaatcc tagcttttca ctgatttcca atactttatc
     9781 caaagcttca atcacttttt taccgaaggg atgtccttgt cctgaatctg cttcatctag
     9841 tacggaagga attcggatca attcgctcaa tgattctaat gcttgttcat gatgttgttc
     9901 tgtcacaaat tttttcatga tattatcctc ctaatatgtt aaaaagggtc atgtcaaatt
     9961 cctccgacac aacctcttct tctcaaataa tgacgtttta agcctgctcc atcagcaagc
    10021 agaatgttct taacaaatga tgaaagaaaa aacactcact gaatggagct attgacttgt
    10081 ccacgtgctt atcgatagcc gtttatcggt ttcttcattc ttatgataga ccttgtacct
    10141 gtatttgtct aggtttagaa cattgctgct acacctagga tcacacagat ggctacaaat
    10201 aatatcgcaa ttaatttacc ggtaaatttc caccacactg aaacatcgaa acgagcaatt
    10261 gctacagcac ccattacgat agcagatgtt ggtgtaatta agtttactaa accagatgca
    10321 gattgataag cagtgattac taaatcctta ccgacgccgg caaattcacc cataggtccc
    10381 atgattccca ttgttgcagc agctaagccg gaagttgaag gaatcaagaa tgacataggg
    10441 atatagaaaa taaatgtaat aatgatgaat acacttgaag ataatgaact taatccttgt
    10501 tcgccccaat gtaagatggt gtctgtgatt aatccattgt tcataaccac ttggatacca
    10561 cgagcaacag ctactacgat tgctacaccg atcaaatcag aagcaccagc taaaaattca
    10621 gagacaaatt cagactcttt catgccataa gtgattgcta ctacgatgga cattactaag
    10681 aataacatag taatttctgg gaagtaccag tcacctaatg gaagcatgct tttacctaga
    10741 accgtaccta gtaccggaag gtttgttaac cagtttgtca atgattcaaa aatcgtgaaa
    10801 ttcgcattca aggttgtcca aggaatcaaa ctgatgatca tgataccaaa agtaataaag
    10861 aacaatacat taacatgttt ttgttttttc gtgattactt cttgacgacc gattgcttca
    10921 atatcgaaat gcttcatatc ttctttacgt tggttgtata ctaatgattt tgttggatct
    10981 ttttcgatct ttgatgcata acgatacaca tatacaatag aaatagctaa caaaataacg
    11041 aacatcaaga atcgaagacc aataccttca cccatcgaaa tcccaacagc ttgtgaagct
    11101 acgcctgttg caaaaggatt taccgttgaa gctagacagc ctacttgaga ccctactaat
    11161 acgattgcta cagctactac tgtatcaaag cctacagcca tcatgactgg gatcagtaaa
    11221 gcatagaatg gaatcgtttc ttctgccatt ccgtaagtag agccgcctaa tgcaaataac
    11281 agcatcaata ctggaataag cattttttct tttcctttga atttcttcac aactgaagca
    11341 atcccggcat ctagtgcccc tgttttgttg ataataccta aaaatccacc aacaaccaaa
    11401 atgaagaaag agattgggat tgcagcaggt gtttctttag ttagctcccc cgctggtaca
    11461 cctagcatcc cattgattgg cgccatgaaa atatcccaca gcccttgagg attttgttgt
    11521 actcgttcat atgatccagc gacgatgttg cctgcatcgt ctacactata gtgcccagct
    11581 ggtataaacc aagtaagtac agcgattact gcaataataa taaataagac cgtatacgct
    11641 gaaggcattt gaaatttttt cttttttgtt tgttccatct tcttcttcct ttcttttccg
    11701 ttagagacaa caattttggc tctaatgtta ttttatcaaa aagagtatca gaacaaaaat
    11761 aataacacgc attttgtaaa acttttgttc aaaaagttcg tttttcccta ttccctcctc
    11821 ttttttcagg tttttaaagc tttcttcttt ttcattgtag cgtttccttt tttttataaa
    11881 caaacatttt cgtcttttaa aaggagaaga atttcacatt tgcaaagaat tgattgattt
    11941 ttatgcataa atcaaaaaaa tactcaaaac aaacaaataa aacgtttaca ttattgcatg
    12001 tcttgtattt ttttccaaaa aaaaagacca agaaatttct tgatcttttt gatatttatt
    12061 tgactgctgt atgacgttgt tcgaactgat gttgttttcg acttaaccat ggaagaagtt
    12121 gcgcatgcag cactaaaaag acagcactca cgatgaatcc tttgatcaag ttgaaaggaa
    12181 cgatcccaat cagtacatag tttgctaaag acatgcccag catctgtgtt gcgttcagcc
    12241 ctaaaaacat taagtaaaga ggcgtaataa cgaaataatt tgcgatactc ataaaaatag
    12301 ttagagcgat ggttcctgta atgacaccta aaaatttgtt tcttccttta tgctgtttct
    12361 ttacactaaa gaaataataa atcgggaatg taaagatcgt tgttgataag aaactagcag
    12421 tgtctcctac caaattatcc ggagaaaaac ctgtagtcag cagatgtaat actgaacgca
    12481 agaacgctgt tgcaactccg gccgctggac cgaataagaa catgctgatc aataccgaga
    12541 tatcactaaa atcgatcttc aaaaaactaa aagccggcat gatcggaaaa gcaaaatact
    12601 gtaaaactaa tgcgatcgca gcaaacatcg ctactgcaac cattttctgt acacgggtgt
    12661 tcttcatttg aaatcctcct gttaggactc ctcttcagcg aactttttga cgtttctctc
    12721 caaatgaaaa aactctattt tccagggaaa atagagttag agttatttga tcaagcgtcg
    12781 tcaaataagc tctatcttct ttatccagac tatactgtcg gtatcggaat ttcaccgatt
    12841 cagctatttt tttgcaaaat aggtcgtgga ctataaccac cggtcgggaa tttcaccctg
    12901 cccctgaaga cgaaccttat ataatttttg tttacatatt cattatacat atctattcaa
    12961 aaaacgcaac atctaacact tataaaaagt aagtgttcat tttgagtggt tcagcaaact
    13021 gctgacttct tttttgttca gttcacggta atccccagga cgtaggcctt gtaaagtcag
    13081 atctccgtat ttttcacgtt ttagtttttg cacaggaaga ccaactgctt gaagcatatt
    13141 tttcacttga tgattacgtc cttcgtggat cgtcaattcc acgatactgt ggtttgtctt
    13201 aggatccact gataaaattt ggaatttagc tggagatgtt tttcgtcctt cgatctttat
    13261 tccttttgtc aaaggtgcaa gcatcgattt tgtagcgatc ccttttacct ttgcaacgta
    13321 aactttatcc acttcatgtt ttggatgtgt caggcgctga gcaaaatctc catcatttgt
    13381 cagtagcaag atccccgaag tatcgtaatc cagtcgacct acaggataga tacgttcttt
    13441 taccatcggc aaaagatccg taacaacttt tcttcctttg tcatctgcaa cagcagaaat
    13501 aactccttta ggtttgtaca ataaatagta accgtattct tcttgataaa ttgggacttc
    13561 atccacttcc acagtgtcat gtttcccaac ttttatccct aattccctta caacttgtcc
    13621 gtttactttt acacgtcctg caaggatgaa ttcttctgct tttcgacgag aagtgatgcc
    13681 agcgtgggca attactttct gcaatctctc cattacaatt attctccttt attcattcca
    13741 tcaaagaata gatctggtga cacgtcttct tcaagtgaaa tttccatttc ttgtgtatca
    13801 ggcagttctt ctaatgattt caaaccaaaa taatctaaga agtagccagt tgttccatat
    13861 aatatcggcc gacctggtcc ttctactcta cctttttctt caatcagttt atgggcagct
    13921 agacgctgta ccgctcctga aacctgaact cctcgaatct gctcgatttc agctcttgtg
    13981 attggctgtt tataagcgat gattgctaag gtttcgacgg cggcttgaga taatgtgttt
    14041 gatgtccctt gcgcatatcg cttaagtaag ggagccagct cttttttagt cgttaataca
    14101 taatggcttt ccgtttcaaa aatatgcaaa gctgagtgcg agttttgagt gtaagattct
    14161 tttaattctt tcactaaact tgtgacttct gcttcgctga gatttaacag tccgcttaat
    14221 tcttcattac tgatcccttc ctcgccaaca ataaaaagaa gggcttcaag ttcacttaat
    14281 ttcgtcatgt tcggtatctc ctactgctgg ataaagcata attgattcat agttttcttt
    14341 ttgttgcgca atgatctgcc cttttttcat caattctagc aaagccataa aagtagtgac
    14401 aacttcactt ttagaaggtg actcaaaaaa agaatcgaaa gtacagccat gcgcatttct
    14461 ttttatacgc tcttcgataa ataccattcg ttcttcgatc gttgtgtcat cgggcgtgat
    14521 cgttttttcc actttttgag aaaatttctt acgttctaag attttctgca tggcacggat
    14581 caaatcgatt tttttgactt gtcctcgctt caacggcttt tcagcatccg ctaacccatc
    14641 gatgttcata ggttcttttg tgtaataaag acttcgttcc tctgcttttt ctgtcagtct
    14701 ttcagctgca tatttatatt tacggtattc taaaagctga gtgaccaatt cttctcgagg
    14761 atcttcttcc cataattctt catcgtctgt gccgaattct tggacaggaa gcaatgtctt
    14821 gcttttgatt gccatcaatg tagcagccat cactaagtat tctcctgcaa cagctagctc
    14881 gagtgttttc attgtatgga tataattcat atattgctcc gtgatcgtag cgatcggtat
    14941 atcatatata tcgatttcca tttgctgaat cagatgaagc agcagatcga gaggaccctc
    15001 gaatacatct aatttaatat tgatctccgt caactcattc actctttctt ctttttcttt
    15061 gctagccatt ttccaatgta aggctctgtg atttcagttg cacggatgga gacatccggg
    15121 tacttttcct gtaattcatc taacatttga aagcccatat tctcaccgcg ataagacgga
    15181 ttcaaagaaa tgtcatggat gatcagctga tcgttcgttg cccagtaaat accaataatc
    15241 ccttgaatgt tggttgaacc ttctggcgta aagaaaaaaa gttcataatc gtcttcagat
    15301 tcgtacgtat caatctcctt caacaaagaa gttttacctg tcagtttctt atggaaactc
    15361 aatagtccca tagcaatttt acggttttct ttccggtaat gtgttaacat cttctcacct
    15421 tcctatgact aagcccttgg aaaatgctgc ttataaacat ccgtcattct tttcttcgtg
    15481 atatgtgtat aaatctgagt cgtagagata tccgcgtgac ctaacaattc ctgtactgtc
    15541 cgcaaatcag ctccattttc tagcagatgt gtggcaaagc tatgccgcag cgtatgtggt
    15601 gttacttctt tgtaaatccc agcctctcgt actaactgct tcagattttt ccagattcct
    15661 tgccgagaaa gacctttccc atgattattg acaaatacat gcatttcaga tgcgtccttt
    15721 acaagtaatg gacgtgcttc gtctaagtag cgttccagcc attggatcgc ataatcacct
    15781 aaaggaatga tccgctcttt atctcctttt ccaactgtct gtaccagtcc aagagaaaga
    15841 tgaagatctc ccaatttcaa tccaatcaat tcactgacgc gcatcccagt agcatacatg
    15901 acttccaaga ttgcacgatc tcttatccct aacgtttttg tcgtatcagg tgcttcaatc
    15961 agcttttcta cttctgtcag agtcaatgta cttggtaatt tctgtgcttt cttcggcgta
    16021 tcgatatgtt gcatcggatc atgatcagtg atacgctctt gccgtaaaaa ctgatggaat
    16081 cttctgagac ttgagatcat ccgagtaaca gttgctgtcg cttttttttc ttcatgcaac
    16141 ttctccagaa aacggatgac taaaaagcga tcgacatcct gccatgaagc aattttctgc
    16201 tcctgtagaa aatgcaagta ctgttccaaa tcacgttcat agctttgtcg tgtattggcg
    16261 gacaatcccc gttcaatatt caagtagtgg agataatcgt tgatttgctc tttcataata
    16321 atccccctca tgcctctcta tcatatcaga taccgcttca ttctgatagt ctttttccat
    16381 aatctgagca aagaaaaagc cgtctagaca ctcagacgga ctggtttctt ttt
//
LOCUS       DACEXQ010000033        15917 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.033, whole genome shotgun sequence.
ACCESSION   DACEXQ010000033 DACEXQ010000000
VERSION     DACEXQ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.033"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            128..1963
                     /gene="tdc"
                     /locus_tag="IXH04_000860"
     CDS             128..1963
                     /gene="tdc"
                     /locus_tag="IXH04_000860"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAQ1851489.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEQVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     EQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKEIAKAMEKKLNEIIQ"
     gene            2268..3746
                     /locus_tag="IXH04_000861"
     CDS             2268..3746
                     /locus_tag="IXH04_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1851490.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLKNPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3712..5043
                     /locus_tag="IXH04_000862"
     CDS             3712..5043
                     /locus_tag="IXH04_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1851491.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRVMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKANFGQLNELVPFGFAGMIASFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKNAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQSGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFVGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQLIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5157..6467
                     /locus_tag="IXH04_000863"
     CDS             5157..6467
                     /locus_tag="IXH04_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAQ1851492.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIEARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADVRLIEVHDLLVE
                     ESILTGESEASEKNHSVLAAEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLILFLVVFTTFRYGMDWHILLSAMIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEETRVLFIMKNCQELKTKEEQKTENLSGNPTELALIRHTETADLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGGVIYVKGAPEVLLAKSTLSSSE"
     gene            6606..7778
                     /locus_tag="IXH04_000864"
     CDS             6606..7778
                     /locus_tag="IXH04_000864"
                     /inference="COORDINATES: protein motif:HMM:NF013991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAQ1851493.1"
                     /translation="MIDPPKESAVKAVKECQQAGISVKMITGDHKDTAKAIAEQIGLK
                     HTAKVLEGIDLDLMSEEELIRQVPKVDVFARTTPEHKLRIVKALQKNGEIVGMTGDGV
                     NDAPALKRADIGIAMGIKGSEVSKQAADMVLGDDNFHTIAKAVKEGRRILDKSAKTLT
                     PFFEEMAQIAWHYAIDQELRERIAEIGRKAEKAMLIATNGINTHKGAIWAMGLLISVI
                     AQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQKYGVNGAYEEAILGY
                     PHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSDQKTLVEMQKLAHAAS
                     QSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELWTADTYICPMLLND"
     gene            7924..8652
                     /locus_tag="IXH04_000865"
     CDS             7924..8652
                     /locus_tag="IXH04_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAQ1851494.1"
                     /translation="MGLCLFALPTQGFAVAGGHGGGTSSGRSSGRGSFGGRSYGGSYF
                     GSRSYGRDSYGYYGPSTGPSSYEQTGFLALAVLSVGGVSAFSIVKNKRQKKRLKSEIL
                     SRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFAEHQKVLQKNKAD
                     GVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLISGSKTQRQYFSQ
                     IWYFDFSEKEKRWQADFIQPIMLD"
     gene            9141..11234
                     /locus_tag="IXH04_000866"
     CDS             9141..11234
                     /locus_tag="IXH04_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ1851495.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLEEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            11259..11561
                     /locus_tag="IXH04_000867"
     CDS             11259..11561
                     /locus_tag="IXH04_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1851496.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            11588..12877
                     /locus_tag="IXH04_000868"
     CDS             11588..12877
                     /locus_tag="IXH04_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1851497.1"
                     /translation="MSVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     ILLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            12896..13303
                     /locus_tag="IXH04_000869"
     CDS             12896..13303
                     /locus_tag="IXH04_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ1851498.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDRHKLAELYEQVGLKFSELHENDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            13326..14195
                     /locus_tag="IXH04_000870"
     CDS             13326..14195
                     /locus_tag="IXH04_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAQ1851499.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANKAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            14197..14817
                     /locus_tag="IXH04_000871"
     CDS             14197..14817
                     /locus_tag="IXH04_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1851500.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            15267..15872
                     /locus_tag="IXH04_000872"
     CDS             15267..15872
                     /locus_tag="IXH04_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ1851501.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
BASE COUNT         5183 a         2695 c         3444 g         4595 t
ORIGIN      
        1 taaaaatcta cgtggatttg atgttataaa aaaaaatttc tcatttttgg cgctatagtg
       61 aagaggtgtt acagataaac acatgtatct gcgagacatt gaccaacaaa atatggaggg
      121 aagaaaaatg aaagatatgg atatcaaggc cgtctttata ggcgacaaag ctgaaaatgg
      181 accagtttat aaaatgttgt tgaacaagat ggtagatgaa catcttggat ggagagaaaa
      241 ttatattcct tcagatatgc ctgcaattag cgaaggagat aagctcactc cagattatct
      301 agccacccgc gaccatatga tagaggtctt ggatgaagtc agtcagcgat tacgcgcagg
      361 atcgatccca tggcattcag caggccgtta ctggggacaa atgaatgctg aaacattgat
      421 gcctgcttta ttagcttaca actatgcgat gctgtggaac ccaaataatg ttgcattgga
      481 atcttctatg gcaacttcgc aaatggaagc agaagtagga caggattttg cttcattatt
      541 caatatgaca gacggatggg gacacattgc agcagatggt tctattgcta atcttgaagg
      601 attatggtac gcccgctgca tcaaatcgat tcctttagct gtcaaggaag tgctgcctga
      661 acaagtgaaa aaaatgtctg agtgggaact tttgaattta tctgttgaag aaattttgga
      721 aatgaccgaa agctttacgg acgaagaaat ggatgaggtc aaagcagctt cttcccgtag
      781 cggaaaaaat atccaaagat tagggaaatg gctggtgcct caaacaaaac actattcttg
      841 gatgaaggcg ttagatattt gcggagtagg cttagatcag atggttgcta tcccagttca
      901 agaggattat cgaatggata ttaatgcttt ggaaaaaaca attcgtgaat tagctgaaca
      961 aaaaatccca attctaggtg tggttgctgt cgtaggaacg acagaagaag gtcaagtaga
     1021 cagtgtagat aaaatcgtcc aattaagaga aaggttaaaa gatgaaggga tctatttcta
     1081 tttacatgtt gatgccgcat atggcggcta tgcgcgttca ctgtttctaa acgaagcagg
     1141 agaatttgtg ccgtatgctt ccttagctga attctttgaa gaacatcatg ttttccacca
     1201 ctgcgtaacg attgacaaag aggtatatga agggttcaga gctatttcag aagcggattc
     1261 tgtcacgata gatcctcata agatgggcta tgtcccttat gctgctggag gaatcgtaat
     1321 caaacataaa aatatgagaa atatcatttc ttatttcgcg ccatatgtgt ttgaaaaatc
     1381 agtaaaggct ccagatatgt taggagctta tattttggaa ggatcaaaag ctggagcaac
     1441 cgccgccgca gtctggacag cacatcgtgt attgccattg aatgtgaccg gatatggtca
     1501 attgattggg gcttcaatcg aggcagctca aagattcaga gaatttctcg atcatctgac
     1561 ttttactgtt aaaggaaaaa caatcgaagt ctatccattg aatcatcctg attttaatat
     1621 ggtcaattgg gtattcaaag aacaaggctg tacagatttg aacgctatca atgaattaaa
     1681 tgaaaaaatg ttcgatcggt cttcttatat ggatggcgat gtttatggtg aacggtttat
     1741 tacttctcat actacattta cacaagaaga ttacggtgat tctccgattc gttttgttga
     1801 aagaatgggt ctaacgaaag aagaatggaa aaaagaacag aaaatcactc tgctacgtgc
     1861 ggctatcatg actccttatt tgaatgatga tcgaattttc aatttttata caaaagaaat
     1921 cgccaaagca atggaaaaga aactaaacga aatcatccaa tagaactgat ttttgtgtga
     1981 aagaataaca agagacatat ggagaacaga caattatttg cttcatcaaa acaattgtat
     2041 ttccccctgt ttttcatata ttcctcgcca agatattctt ttttataaaa cgttctataa
     2101 gttggatcgt tcgtttcatg tatcaaaccc agttcactaa gatcgttcaa tggatgtttc
     2161 ggtgatactg ggttttgtac tgaatcgatg aaaccttgtc atttctgttc ttgaacaaaa
     2221 ataaaagaaa acaaaaacat atgtcgctat tgaaagaagg aataaagatg aaagatactg
     2281 gttcatcaaa gcaaatcacg accatgacct ttatcggaat gacctgcgcc cttgtagcaa
     2341 gtgtacgaaa tattccagat gtagcagcaa cgagctggac gatgattttt tacatgctgg
     2401 ttgcagtatt attgtacgct tttccaatct cattgatctc tggtgaattt gccggaatgt
     2461 ttccccaaaa aggtggtcca gaactttggg tatcgaatgc tctaggaaaa aaatgggggt
     2521 tcgttgtctc atggctgtta tgggtccaaa tgtttcctgg gatggtgatg gtcgcttcag
     2581 cacttgctcc gttgtttggt aatattatcg ataatgtgcc gcttggtttg aacagcaaat
     2641 ttacattagt tgtcatttta gtcgtttatt ggatcatcac ttttttgaat ttgaaatttg
     2701 atatggcaaa aatcggcgga aaagtcggtg tttggttagg attgtacgtg cctttgacga
     2761 tgatgctctt attagggttt gctgcgtgga tcaaagttgg gatcgtacca accggaacat
     2821 taggcagttt ttcatgggac aaattaatac cagatactac gactgcatcc tcatttgttt
     2881 attttgcgcc gatcatgttt atttttactg gtatcgaaat gtcctctgtg tatatcactc
     2941 ggttgaaaaa tccagttaaa acgtatattc gcggagtatt tgccgcgtta atttttatat
     3001 ttttcgtcaa tattttgaat gctttagttg tagcaaatgt cgtaccaaaa ggacaaatgg
     3061 aattgaataa tatcgcgcaa tctatttcga tttattgcca aattcttggt ttgccacatc
     3121 ttattgtgaa tctgtttagt ttacttgtct tcattggtgt agcagtccaa ttatctgctt
     3181 gggcctcagg accagcaaag acagtgacag aaagtgctag aaaaggtgcg tatccaccaa
     3241 aattcaattt ttggaagacg aatcaatttg acgtatccaa atccgttatt ttgactcagt
     3301 ctgtcattat ctcaatcttt gcactttttt atttgctgat tcctggcgtc aatcaagcat
     3361 ttttgatgct cgtaaattca actactgtta tctactgtat cgtctatgtc atcatgggga
     3421 tcgcagtttt gagattgcgt tatacacatg caaagttgaa tcgaccattt agaattggaa
     3481 aaaaagagaa aaagagcaat cttggagttt ggactgtcgt tattgtttta tttgcggcaa
     3541 tcggattttc tgtcggactg acgatgaaag ctgggacatg gatcaatcta attgcagtca
     3601 cagccatcag tgtcattttg tttgtagtac cactctgtat tgaaaaaata aagaaacctt
     3661 cttgggaaca ggaagtcgcc atgctgcttg aaaaagaaaa aggtgaactt gatggaaaaa
     3721 ccggcaatga aacggactct aactaatcga aatatccagt taatcgcatt aggtggcgca
     3781 attggaacag gattattttt aggttcagca aaggcaattg catcagcagg gccagctgtt
     3841 attctcgttt atttgatatg cggtttggct atttatggtt tgatgcgggt catgggtgat
     3901 ttatttttag caaatagtac atttaactct atcagtgatt tcgttgacta ttatttaggc
     3961 aaaaagtggg cattttttgt caattggact tattggcttt gctggcttgg agctggactt
     4021 gcggaattaa cagcaatagg attgtatatc catttctggt ttgtacatgt accagctttg
     4081 ataagcggag gtagcgcact aatgatctta atggcagtca accttgttgc agtcaagtgg
     4141 tttggtgaat tggaatcttt tttctcaatc atcaagatct tggggatttt cctttttatc
     4201 ttattgggta taggattgag tgtctcagat tttcataccc agtctattgg aatcaaagca
     4261 aattttggac agctcaatga attagtcccg tttggctttg ctggaatgat tgcttcattt
     4321 cctatggttt tattcagttt tgctggaatt gaaatgatcg gattaacagt tggtgagacg
     4381 agtgacccac aaaagaattt gaaaaatgca atcaactgtc tgccttggcg tatcctcttt
     4441 ttctatatag gaacgatttt gtcgatttta tttgttgttc catggcattg tttagattta
     4501 aaagaaagtc ctttagtgac tatgctgcaa gtgatccatt gccaaagcgg agcagcaatt
     4561 gtcaatctaa tgattttggt tgcttctttg tcctcagcaa atagtgcagt ctacagtact
     4621 agcaggattt tatatcaaac agcaaaagag aaaagtttgc cccattggtt ttgtgcgctt
     4681 tctaagaaag gcgtaccttt acacggtatt ttaattacag ggatgttatt ttttgtcggt
     4741 ctttgtctac atttttttgt agctgatagt cgattgtttt ttcagctttt ggcaggcatg
     4801 acaacgagtt gttttctatt tgtgtggtct tcgatcttgt gtgcacatgt tcaattaatt
     4861 aaagaaaaga aacggaacac tacacaaaga tataaagatc ttgctttgtt ggtgtttttt
     4921 acttcaattg ctttcagctt gttattcgaa agcacgacac gctttattcc ttttttattg
     4981 gttatctggt ttgtattctt aaattttgta tataaaagaa taagcaatag aaatatcagt
     5041 taggcccaga tacattctgc aaaacgaatt atttcagacc cccctgaaat aatcgatacg
     5101 cctgaaaaaa cttccctgcg tataataaca gtatcaatta aacaggaggg gataggatga
     5161 actggtacag attgacggta gaagatgtat tgaaaaacta tcaaacatca acagaaggct
     5221 tgtcgcaaga agaacggcag aaacgcctga ctgatcaagg atataatgag atagaagcaa
     5281 gacagcagac aaaacgatgg aaaaaaatag caaaacactt tactgatctg ctgatgatcg
     5341 tgttgatcat tgcctctttt ttgaaatttg cttcgagcga atacatcgaa ggaagtatta
     5401 ttttattcgt agtgatcgtc aacggactcg ttagttattg gcaagaaaga aaagcagaag
     5461 aatcgttgaa tggtttgaag cagctgatgg gacaagaagc gatagtacta tcgaacggga
     5521 tgcaagaaaa aatccctgcg aaagaactag taaaaggaga tgtcattgtc ttacgtgccg
     5581 gagacgtcgt tccagcagat gttcggttga tcgaagtaca tgatctacta gtagaagaat
     5641 cgattttgac gggagaatca gaagcatctg aaaaaaatca ttccgttttg gcagcagaag
     5701 aatcaatcgg tgaccaaaaa aatatggctt tttcaggaac gcttgtacaa tctggttctg
     5761 cagtcggtgt cgttgtagaa acaggaaaca cgacagaaat cggtaaaatc aatcaagcac
     5821 tgcaatcggt cgaacaacaa acgacgcctc tgatcaagaa gatcaaccga ttgaacaaac
     5881 aaatttttca aggcattctt tgtttgatct tgttcttagt tgtttttaca acattccgct
     5941 atggcatgga ttggcatatc cttctttcag ctatgatcgc tttggtggtg tcaatggtac
     6001 ccgaaggatt gccagctgtg ttgaccatga tcctatctgt cggagtacat gaaatggcaa
     6061 aagaaaaggc gatcatcaaa ggactgcctt ctgtagaaac attaggttct atgacagtga
     6121 tctgttcaga taagacaggg acattgacaa aaaatgaaat gacagttgtg gatgtagcag
     6181 caaaggaaga aactcgcgta ctgtttatta tgaaaaattg tcaggaatta aagacgaaag
     6241 aagaacagaa aacagaaaat ttaagtggca atccgacaga acttgcactg attcgccaca
     6301 ccgaaacagc cgatctgcct ttgaaaaaaa tagaagcaaa aattccgttc agctctgaat
     6361 acaaatatat ggcaacgatg catgcggagg aaaaaggagg ggtgatttac gtcaaaggtg
     6421 cgccagaagt attattggct aaatcaactc tttcttcttc agaataagaa gcatggggtc
     6481 aaacagccac agcgtttgcg aaaaaaggtc agcgtgtttt aggatttgct tacaaaaaag
     6541 tagattcgaa acaggaactc acgcatgagt ctttaaccca attaactttt gcagggattg
     6601 ctgggttgat tgatccgcca aaagaaagtg cagtcaaagc tgtaaaggaa tgccaacaag
     6661 caggaatctc agtgaaaatg attacggggg atcacaaaga tacagcaaaa gcgatcgcgg
     6721 aacaaattgg cttaaaacat actgctaaag ttttagaagg gatcgatttg gatcttatgt
     6781 ctgaagaaga gttgattcga caagtaccaa aagtcgacgt ctttgctcga acgacaccag
     6841 aacacaaact gcgaatcgtg aaagctttgc agaaaaatgg agaaatcgtc ggaatgaccg
     6901 gagacggcgt aaatgatgca cctgctttga aaagagccga tataggaatc gctatgggaa
     6961 tcaaaggtag tgaagtaagc aaacaggcag ctgatatggt tttaggagat gataatttcc
     7021 atacgatcgc aaaagccgta aaagaaggtc ggcgcatttt ggataagtct gctaagacgc
     7081 taactccttt ttttgaagaa atggctcaaa tagcatggca ttatgctatt gatcaggagt
     7141 taagagaaag aattgctgag attgggcgaa aagcggaaaa ggcaatgctg attgctacaa
     7201 atggcatcaa tacccataaa ggagcgattt gggcaatggg gctattgatc agtgtcattg
     7261 cacaaaaaat cagtatggaa cagccttttt ctttatcgaa aatacttgat gatgtcagca
     7321 agcttgctgc ttttcctgat acaaaatatc gtcataaaaa aaccactcat ggtcataggg
     7381 tgaagcaaaa atatggcgta aatggtgcat atgaagaagc gatattaggg tatccgcata
     7441 tcaggcttgc aatccaagca gctaaggagc tagataatga ttcatatgta cagaaacaaa
     7501 tgcatatgtt gttagttttg atgagttcgc tagatgacac atgcgtattg catcgcagtg
     7561 atcagaaaac gttagtggaa atgcaaaaac ttgctcatgc tgctagtcaa agtgctttac
     7621 caaatcgtcc atttacagca ctgttgcact tttgtcagga aaaccaaatc tctcctggag
     7681 gaagcgcaga tctcttgtct gcaagcttgt tcatgctgaa gatagaggaa ttatggactg
     7741 ccgacacata catctgtccg atgttactca atgactaaat aagacaaaat tgctaaaaaa
     7801 acagtttatg catctggtta ttgactcttc cttttagcaa ggctatgata aaaatagttt
     7861 tttgtaataa aagataacga ggaagacaat gaaaaaatca attatttagc aatcattttg
     7921 ttagtgggcc tatgtttgtt tgctctgccg acccaaggat tcgctgtcgc gggtggtcat
     7981 ggtggaggta catctagcgg tcgaagttcg ggcagaggtt catttggcgg tcgttcatat
     8041 gggggcagtt attttggcag tcgttcttac ggcagagatt cttatgggta ttatggtcca
     8101 agtacaggtc cttctagtta tgagcaaacg gggtttcttg cattagctgt tttgtcagta
     8161 ggaggggttt ctgctttttc tatcgtcaag aataaaagac aaaagaaaag gctgaaatcg
     8221 gagatattgt cccgcatgtc aggaacggct aaagaaaaga aagcacagtt tttggaaatc
     8281 caaaagatat tcatgaagat ccaatccgct tgggaagaag aaactcctga aaaagccaga
     8341 agctgttata cagacaagct gtttgccgaa catcagaaag ttctgcaaaa aaacaaagca
     8401 gacggtgtaa gaaatcatac taaaaaaata actattcagg aagtaggaaa ttatcgacag
     8461 atccacgaaa atagttttgc catccgaatt gatttcagat gctgtgatta ttcagtcgat
     8521 cgaaaagatg ggcgattgat ctcaggaagt aagacacaac gacagtattt ttctcagata
     8581 tggtattttg atttttctga gaaagaaaaa agatggcaag ccgattttat tcaaccgatt
     8641 atgctggatt gacaggaaat atttatgaac ggagttgtga caaaagcctt gtcgcagctc
     8701 ctttttctga ataaacggtg ttccagagat agcctatcgg caataaggca cgattttgta
     8761 aaatatggga agcgattttt caaaatcgct tcttatcgct caaggctgaa catctctgtc
     8821 acagcttctc cactaaatgg tgtttaaaag cttgcatctg cgataataag aacaaccaaa
     8881 caaaaaatag gaagcactat tttagtttag ttgttcttat tacttgatgc aggacagctt
     8941 tttcacaacc tcttttttgt atagatgggc aaaatttttg taataatagg cgtatgtctt
     9001 tttcgttgag gagatgaaaa agcatatggc ttattttttg tttgctttgc ttactttctt
     9061 tccacttcaa gtggcagaaa tatctacttt ttattatgta agcggaacca tatacttgaa
     9121 atcagaagga gatcatttct atgaaaacaa gaacaattga aatacttcac agaatcatga
     9181 cagcccaata tccagtaaaa gcaaaacatc tagcaagtga gttcaatgtc agtcagcgga
     9241 cgattcgtca ggaagttctt gaagcgaata tctggttgag cagtaaaaag cttccggaga
     9301 tccgtacaat tcgcaacaaa ggttttttgt tgaagctgga aaaggaaaag caagctcgat
     9361 tagaaactga aataaaagat gtgaaagacg gctttttgaa tcggcgcgaa cgtatttttg
     9421 atctagtttt agccgttgct tacagccgaa tcccgatcca tctaaacaaa aaagaagaag
     9481 aatttcaaat ctctaaaagt acattagatg aagatatgag acggttacga gcaacactgg
     9541 tcagttatgg gatagagatc gtcagttatg gaaagcaagg actggtttat aaaggttcag
     9601 aacgatctat ccgtacgatg atctatgatt ttatcaatca gaatctggga atgaccgact
     9661 tctttatgaa tcaagatgca caattaacac cggctcaaaa aatctttctc cattattttc
     9721 ctttggagga aatcaaaaaa atacaggaga tctattccaa taagttgttg aaacaagaag
     9781 atgatattta taaaaaacaa atcctgcttt ttacattgat ctggatccag cgaatcagga
     9841 aacatgaatt tatctctgcc attaattgga aaaatacaga aataaaagac agtagctttt
     9901 ctgcatttat tgaccacatg atcgaggaat ttcaattgga caatgtcccg caagtagaaa
     9961 gaaattatct tcgtttcact attgagacgt tcaatgctag agatatctcc aattcaatcg
    10021 agtgggtaca agctcaatta ctaacgatcc aattgatcca atttgtagag aacgagacac
    10081 acgtaccttt ccatctaaag gaagaagcct tattcgaaaa tctctataag cacatggctg
    10141 cactgattgt aagaatcaag aatcatatcc aagtcatgaa tccattgaaa gaaaacatcc
    10201 gaacaaatta tttcccaatc taccgcgcag tcactcattt tgttcctacg atcgaagaag
    10261 taattggtgg caagatactg gaggacgaga tagcttttct tgtcatccac ttttcgacga
    10321 tcgccagtgt aatcaagcga gacttgaatt atgtttataa atcagtcgtt gtctgtaacc
    10381 atgggatggc tactggaaat ctgttagcag agaatctgaa agaaaagttt cctcagatcg
    10441 agattactgc agttttaagt tcaaaagaaa tcgaattagt agataaattg gatgtcaatc
    10501 tggttttcag cacctttcaa ttgaattatc gaaataagcc gcttttagtt gtcgatccga
    10561 ttttaactga gacgaatatc ttggttatag aagacttttt gaaaagaaat gaagaactac
    10621 agcggttaga gcctaacagt catgaatcga cggagttatt cactcaaata atagatgtca
    10681 tgaaagaaag tggtggccga gtagatcgaa tcatctatgg caagttagaa aaattattcg
    10741 aaacaaacaa tttagaaatc aacaaaaggg agatccagcc tatgttaaaa gatattttga
    10801 cggacagcaa tatcatgatc aaagaaaaag ccactacttg ggaggaatcg attgaactag
    10861 ctgctagacc attggttaaa gaaaacattg tcgaagagcg ctatgtcgaa gcgatgattc
    10921 gtgcagtgaa tgattacgga ccgtatatcg tgattggcaa acacatggca ctagcccatg
    10981 cccgaccaga agatggggtc aataaattag gagtcagcgt agctacgatt gagcaaccaa
    11041 tcgatttcgg taatcctgaa atggatccag taaagattat tttttgtttg gcagcagtag
    11101 actcctactc ccatctgaac atcatgaaag aactaattga attgatcaac aacgaagaga
    11161 agttggatcg attgattgaa tgcacaaata tcgaagattt caaacaactg cttttttcag
    11221 tagttgagaa ataataaatg atacggggga gaacaaagat gaacaaattg aacattttat
    11281 tcgtatgcgg cgcaggttta ggaagtagtt ttgcagcaca gatgtctgct gaggatgttt
    11341 tgaacaaatt aggagtagaa gccaaactgg accattgtga tatttcttcc gcagtttcca
    11401 tgaaaccaga tgtgatcatc acagcagaaa attttcgttc gcagttcgaa aagttcgcag
    11461 ttgatcctaa aacggcgatg atctatttga aaaatattgt ttcaaaaaat gaaattgaag
    11521 aaaaaatcac cccagttctg caagataagg gagttatcta aatacaaaaa gaaaagaggg
    11581 taaaaacatg agcgttatca attttatcat cgaaaatatt ttgactcagg catcgatcac
    11641 cattgcattg attgctatgt tgggtctact actgcagaag aaatcagcag gacaagtcat
    11701 ctctggcacg ttaaagacac tcctaggatt tcaagtgttg agtgccggtt cgagcatcat
    11761 cgttggaagt ttaacttatt tcgggaaaat ctttactgaa ggatttcata tgcaagggat
    11821 catcccttct attgaatcta tcaatggtca agcaatgaat gatttgggtt taggtagaga
    11881 tattgcactg acgttcttag ccatttttgt attcaatatt attttggctc gtttcacaaa
    11941 atggaagtac atttttttaa ctggtcaagc aattttatgg atggcaacga tgacgacagt
    12001 ctttggctat tttgccggtc tgcgagggat cgtattgatc ttggtcggtg gttttatcgg
    12061 agcttgtttc gccattgcta tgccggcagt agcgcaaccg atcattcgta aaatcactgg
    12121 atcaaatgat attgctttag gtcatttttg tacgatcggg tacttatttg aagccggtgt
    12181 tgctaagctt tttggtgaga aaggcgaaaa taaaaagtcg atcgaagata tcaagttgcc
    12241 gacacatttc gaatttctgc aagatactta tttgtctgtc atggtcgtga tggtcccgct
    12301 atatatcatc actgttttat ttgcaggcga accattcgct tctgaattat caggagatca
    12361 aaactatatc atgtttgcct ttttacaagc catccaattc gtagtgggtg tgtatatatt
    12421 gctagctggt gtacgtctac tgttaggtga aatcgtgccg gcatttcgag ggatagctat
    12481 gaaattagtt cctgatgcga tccctgcctt agattgtcca gttttctttc cttatagccc
    12541 aaatgccgtc attttaggtt tcattacgac aacgattggt acgattattg ccatgtttac
    12601 tttgcccatg ttcggcttag cgatgatttt acctggtatg ctaaccaatt tctttgccgg
    12661 agggacagca gggatatttg gcaacgcagt tggcggacgc cgaggagcga tcattggcgg
    12721 tattgctcat ggtttcttta ttacattatt gcccgctttg ctagtaacga tctttaattc
    12781 gatgggattc atcaatgcta cagcaaccga tgtagataca gtagccgcag cactcttata
    12841 tgcctggatc ttaagcccag ttttgaaagc gttttaattg aaagtaggga aaatgatgtt
    12901 tggatttttt aagaaaaaag aaaaagaacc gaaaaaagtt ctggcaaaag aagaaaaagc
    12961 cctatcgcca gaagcgaccg aaaagattca atcagagatt acacaattga aacaagagat
    13021 ttccactacc caagataggc ataaattagc ggaactttat gaacaagttg gacttaaatt
    13081 ttctgaattg catgagaacg atcaagccat ccaatattta gaaaagagct tggaaaataa
    13141 acagacgatc ggagatgggt ataaaaaact gatgagcctc tataaccaga agcgtgcaga
    13201 tgctgctcgt gctggtgatg atcaagggat cgattactac atgggcaaaa tggatgagat
    13261 gcgacaaatt gcaaaacaag taacgatcaa aggaaacaaa taaaaagata aggtggcaat
    13321 aaaagatgta tacaacattg aaagaagtaa caaaaacagc agaagagttg aattttacag
    13381 ttggagcttt taatacgcat aacctagaaa tgcttcctgc catgctaggc gcagcaaaag
    13441 aaatgggttc gccaatcatt attcaaacaa gtgtcgatac cgctaaatat atcggcatgc
    13501 atgtgttagt caatgtaatg acagctattg cagaagaaga aatggttgat gcagtcttac
    13561 acttagacca cgcccgagac tttgatgata tcaaagaagc aatcgatagc ggatatactt
    13621 ctgtcatgta tgacgggtcc catctacctt tcaaagaaaa tatcttgaag acaaaagcag
    13681 tagtagaata tgcccatgcg cacggtgttt ccgtagaagg agaacttgga acaatcggcg
    13741 gtacagaaga aggaatccat gtcgctgaaa atgacaaggt atatacaaga ccggaagacg
    13801 cagtgaaatt tgtagaagct acaggcgtgg atgcattagc tattgctatc ggtacgaatc
    13861 atggacaatt caaatcaaaa acagaagtaa acattccctt gctgaaagaa atcgatgcag
    13921 tagttgatgt ccccctagtt atccatggag gaacaggtgt gaaagaagaa gattatcctg
    13981 aattgatcaa taacgggatc cgcaaattca atgttggaac ggagctttta gtcaactgga
    14041 cgaaagtagc taaagaaacc tttagtgaaa cagaaatcaa caagtctctt cgccataatg
    14101 tgattccagc aaataaagca gtaaaagaaa ttgtcaagca taagattgga ctgtttatga
    14161 acttggaaag tccaattaac ttaggaaata aatgagatga aaaagtattt cattcttctg
    14221 gctggtagtc ctggtacagg aaaaacatat cttatgaata aactacggga acgatttcca
    14281 gatatgtatg ccatcacgct cgatgaaatc aaggaatact atgcagaatc catcgggttt
    14341 aacaatttgg aagaacgggc ggaacaagaa aggacgaaag tttatccttt ctattacaaa
    14401 gcacttgatt tgtatatgga agcaggaaag aaagtgattg tgtccgagta tccttttagt
    14461 gataagcaaa aagcacgact ccaagaatta gcagatagat acggatatca ggtgatcacg
    14521 attcgcctaa cagcagactt tgaagtgtta tggcaacgtc ggtacaaacg tgatcgtgaa
    14581 tcagatcgtc atttgagcta tatcatggat cattatcact atggtgacgt attggaagat
    14641 cgaacgaaag ggaccaatca tattacgaaa gaagcattca aaaagatcat cgccgaccga
    14701 aaatatgaca aattccagtt aggaacacta tatgaatttg atgtaacaga ctatactcaa
    14761 gtggattaca ctccactatt ggataaatta gaaaaagaaa tcaatgacaa taagtaacaa
    14821 agaaatggct taatatcgaa aaattggctt ttgaacaccg tttaccgaga ggtcgtgagc
    14881 ctgtcgttca gctccgagtc acaaggagca atttcaaaga aacagcttct catgtttgat
    14941 gaatattgtg acttgtgacc gaggagttgg taggagaaca ccgtttagtg gggaggttgt
    15001 gaaaaagctg tcctgcctca attaataaga acagccagac aagaatagct cgtcatattt
    15061 ttcgtttgat ggggcttatt accgcagatg caagcttttg aacgccattt attcacagcc
    15121 ccagagaagt cgaatgtctt ttgttctcga gttttttaac attcttttat ttattgcaga
    15181 tttcattata tttatttgcc gtaattaaac cagtcttcta tacttgatta catagtcata
    15241 catttgctaa aaaagaaggg aagagaatga acaagaagtt ggctcaatac aaaagactac
    15301 tcgcactttc tcaagctggt ctatattatg gaaaagatgt atttgataaa gagcgttttg
    15361 aggagatccg gacgatttca ttggaactga tcagtgaaat aggaaaagaa actttcgaag
    15421 aaataaaagc gctcacaacg attggagaag gacatccaac accaaaagta gatgttcgtg
    15481 cctatatcaa aaaagatggc aaggtcctac taatcgaaga taaacggacg aaagaatggt
    15541 cacttcctgg tggatttgct gaaataggat tgtctcctga agaaaatgtg cgtaaagaag
    15601 tctatgaaga aacaggatta acagtagaaa ctactcagtt gcgagcagtt tttgatacga
    15661 ataaacaaaa agatattcca cagctttttc agtattataa acttgttttt gcttgtgcga
    15721 tccacggtga agaggccaag tttattgaga ataatgaaac ttccaacatg ggatttttta
    15781 gtatagaaga gttgccaaag ctatcagaaa agcgaacaac gaagaaacag ttacttattt
    15841 tagaaaacaa aaaccagatc tattgtgatt gagctttttc gtaaaagaag aaaagaatat
    15901 actttactag ttattaa
//
LOCUS       DACEXQ010000034        15853 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.034, whole genome shotgun sequence.
ACCESSION   DACEXQ010000034 DACEXQ010000000
VERSION     DACEXQ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15853
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.034"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            293..1432
                     /locus_tag="IXH04_000873"
     CDS             293..1432
                     /locus_tag="IXH04_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAQ1851502.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1499..2074)
                     /locus_tag="IXH04_000874"
     CDS             complement(1499..2074)
                     /locus_tag="IXH04_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1851503.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2079..3053)
                     /locus_tag="IXH04_000875"
     CDS             complement(2079..3053)
                     /locus_tag="IXH04_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAQ1851504.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3077..3121)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3336..4304
                     /locus_tag="IXH04_000876"
     CDS             3336..4304
                     /locus_tag="IXH04_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1851505.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     gene            4417..4695
                     /locus_tag="IXH04_000877"
     CDS             4417..4695
                     /locus_tag="IXH04_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ1851506.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            4813..5049
                     /gene="secG"
                     /locus_tag="IXH04_000878"
     CDS             4813..5049
                     /gene="secG"
                     /locus_tag="IXH04_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAQ1851507.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            5161..5907
                     /locus_tag="IXH04_000879"
     CDS             5161..5907
                     /locus_tag="IXH04_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ1851508.1"
                     /translation="MRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSLAEQ
                     QLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5910..8267
                     /gene="rnr"
                     /locus_tag="IXH04_000880"
     CDS             5910..8267
                     /gene="rnr"
                     /locus_tag="IXH04_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAQ1851509.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWATELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8289..8753
                     /gene="smpB"
                     /locus_tag="IXH04_000881"
     CDS             8289..8753
                     /gene="smpB"
                     /locus_tag="IXH04_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAQ1851510.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            9055..9774
                     /gene="atpB"
                     /locus_tag="IXH04_000882"
     CDS             9055..9774
                     /gene="atpB"
                     /locus_tag="IXH04_000882"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAQ1851511.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            9827..10042
                     /gene="atpE"
                     /locus_tag="IXH04_000883"
     CDS             9827..10042
                     /gene="atpE"
                     /locus_tag="IXH04_000883"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAQ1851512.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            10133..10657
                     /gene="atpF"
                     /locus_tag="IXH04_000884"
     CDS             10133..10657
                     /gene="atpF"
                     /locus_tag="IXH04_000884"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAQ1851513.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            10644..11186
                     /locus_tag="IXH04_000885"
     CDS             10644..11186
                     /locus_tag="IXH04_000885"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAQ1851514.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            11209..12765
                     /locus_tag="IXH04_000886"
     CDS             11209..12765
                     /locus_tag="IXH04_000886"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAQ1851515.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            12779..13681
                     /locus_tag="IXH04_000887"
     CDS             12779..13681
                     /locus_tag="IXH04_000887"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAQ1851516.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            13704..15110
                     /gene="atpD"
                     /locus_tag="IXH04_000888"
     CDS             13704..15110
                     /gene="atpD"
                     /locus_tag="IXH04_000888"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAQ1851517.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            15124..15546
                     /locus_tag="IXH04_000889"
     CDS             15124..15546
                     /locus_tag="IXH04_000889"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAQ1851518.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         5264 a         2840 c         3419 g         4330 t
ORIGIN      
        1 ttttttttgc aactagcttc tgtatatgat gtttacaaag aatggtacaa gaaaagatca
       61 tccttgaaaa aggaaagcta acaataacta gcatcatata agagctttta aggatagaac
      121 agtagaatag gaatctatga gcaagtcctc tcgtataaat aaaataggag ggaagtagca
      181 gcatattttt cataaagtgg ctctctttct tcatttttac tttggttgct cctatttgaa
      241 aattcgagca cactcaaata caataaaaag aaatggcaag ggagaggatt agatgaaaaa
      301 agaagaaaaa atcgcgattt tacaagagat catccgaatt aaatcagtga atggaaacga
      361 aggtgaagta gcagcctatt tgaataagct tttggcaaag catgacataa caggagagat
      421 tgtttcttac agagatggta gagataattt gattgctcgt taccaaaagg gccaatcagg
      481 aaaagtgtta ggcctatctg ggcatatgga tgtcgtggcg gctggtgatg aatcgtcttg
      541 gacctatgca ccctttgcag cagaaatcca tgggaaccgt ttgtacggac gtggtgctac
      601 agatatgaag agtggcttgg cagcaatggt gattgccatg atcgaactga aagagtcagg
      661 aaaaccattt aacggaacag tcaaattatt agccactgtc ggagaagaag tgggtgaact
      721 aggtggggaa cagctcacga aagcaggcta tgtagatgac ttagatgcct tgatcatcgg
      781 agagccaact aattacagtt tgatgtatac gcacatggga tcaatcaact atacagtcac
      841 ctcgcacgga aaagaagcgc atagctctat gccagaccaa ggttataatg caatcaatca
      901 cctaaatgaa ttcattacga aagcaaatgc tgaaatgaat cacctcgctg aaacaatcga
      961 gaatcctgtc ctaggaaaaa caatccataa tgttacattg atcagtggcg ggaatcaagt
     1021 caacagcatc ccaagccatg cgcagctcca agggaacatt cgatcgattc cagaataccc
     1081 aaatgataaa atcattgcat tgctgcaatc aatcgtcaat gaattgaatc aagagacaga
     1141 ctatcatttg gaattgatga tcgattacaa caaaatacct gtcaaagctg atccagattc
     1201 tcctttgatt cattgcattc agcaacaatt cagccagcca ttacctttag ttggagcggc
     1261 agcaacaacg gatgcagccg aatttacgaa agctaaccat tccttcgact ttgtcgtctt
     1321 tgggcctggt gtcgttactt tgcctcatca agtagatgag tatgttgaaa tcgataatta
     1381 tttggatatg attgaaaaat atcaaggaat tattttatcc tacttagcgt aagatttttt
     1441 taataaatga aaaaacagag ccagattagc attattttgc taatccagct ctgtttattc
     1501 agcctatttt tctttttgaa tatatcaagc gttgtggcaa catagtgatg actaatgaaa
     1561 caacgacgat agacaacaaa cgatcaaggt agtctgtacc tgcctgtacc aagatcacac
     1621 ttgttgtctg acttaaacct aacccatgta atagctgaac gatcatactg gatcctgagg
     1681 aggtgatccc gccaaataac tgaacggtaa tgatcgaaga aacgatcgtt ccaggtaccg
     1741 acaaaatcaa agcgagtaat aacagtttca agctgttttt atctttgccg agatatcttg
     1801 gcaaaatcaa tccagcaagc aagcctgtga gaatttggat tggcgaataa tacagagaaa
     1861 aaatatctgt agtcactcca cttaaaatac cgctggacaa tcctgtcagc attccaggta
     1921 caggcccaag caatgcacca gcaaaaatcg ttccgataga atcgagatag attggcaaac
     1981 gaagccataa agcaatcgtt cctccaatat aattgatccc aatacacaaa gccatcactg
     2041 taagtacgcg agttgccatt ttgtttttat tcattttgtt agcccatctt taacctttcc
     2101 aaatctttca taatcgtttc ttcttgtgca tttaaaagaa cggttaaaaa ctttcggaaa
     2161 aaaagacgag tatctacttt tgtcataatt ttactgttag caggtttctg ccaaaattca
     2221 taacgatcaa ccaatgtttg tccacgactt attccttgag tttccacagc agtgaagctt
     2281 ttgaaacctt cacagatggc cccctcgata aaataagcaa cagctaacgg atcattgatg
     2341 acacatccta aaattctttc ttgtttccag tggaagtcaa aataaaaacg agtgatcgct
     2401 tttagatact ctccctcttc aggattcatt tgacagcagt attcgaggat agtcggtgtc
     2461 aagacgattt ctcttgtcac atctaaccca accatttcta tcgtttgttt gagattctcg
     2521 aatacataag aagctgcttc aggatcacac cagtaattgt attcagcaac tggcgaacag
     2581 ttaccatggc ttttgtaagt tccacccatc gagacaaaac gatccatatg tttcccaata
     2641 tttgggttga ctttcaaagc aaaagcgata ttggtcaaag gacccaaagc aatgactgat
     2701 gtatttgtct tttctttgaa ataatcagcc aaaaaatcaa cggcatggag aggttctgcc
     2761 tgttttctca agatcatcgg gaaatttgtt tcccctaaac catccatacc atgagtgtct
     2821 tgtgcactga tgaaaggttg cttcaaaggt ttttccattc cttgataaac aggaatgtct
     2881 aaacgatcac agcgttccag gcatttcagc acattctgcg tacctatatc tgcagggaca
     2941 ttcccacaaa ctacggtaat agcaactact tcgatatcag gggatttgac agctaatgat
     3001 aaagctaaag tgtcgtcgat ccctggatcg caatcaataa ttacttttcg cattatttcc
     3061 aatctccttt ctttagagat acttagtttt ttattctggg aagtttccga accagtccaa
     3121 gacgagcgtc ttgattataa tgcacgaaat tagtatactg gcttttattg cgaatgtaaa
     3181 tgctctctta caagtaaatc tattgaggag aactttaagt aaatgataca atgaaaaaga
     3241 aatggaattt acgtgtgccg aaaataaaga atcctatgga tcggtacatt ggataatagt
     3301 tagattatag tttggcagaa agcggcgggt gtacaatggt tattttttat ttcattctta
     3361 ttctgataat tatagggatc agttaccatt ttctttctta tttttatgca aagaaattag
     3421 taagagcggg agtacagaga ggatatgact ggtatgaaga cactggtcat cgtttgatgg
     3481 accctgctgc agtcacgtca gtagaaaaaa aacgacaaaa attagaagaa gagtgtgaaa
     3541 gcttttggga gcagggaaca cccaaaacaa tcaaaagtta tgacgggttg aaactagctg
     3601 gtcagatgtt catccatcca aataagcaga ataaatgggt catttgcgta catgattacc
     3661 gaagcacagg gaaaagagac atgtctcata taggtaaaag atatgcagag aaaggattca
     3721 acgttcttgt acctgattta cgtgcgcacg gagaaagtga aggagaaatc atcggaatgg
     3781 gatggctaga tcggctagat ttaattgcct ggatccaact catcttagat gaacagccga
     3841 atgcgtccat tattttacac ggcggatcaa tgggagcttc tacgatcatg atggcaagtg
     3901 gtgaaaaact tccatcagcg gtcaaaggat ttattttaga cagcggatat gtttctgttt
     3961 atgcagaatt ccgatatatg ttaagtaaaa tcactgtctt tcctaaaaaa atggtcatga
     4021 gatatgcgaa tcattatgcc cagaaatacg ccggttactc cttaaagcaa gcatcagcta
     4081 cgagacagct tggcagcaac catctgcctt tattggtcat tcatggtgaa agagatcatt
     4141 ttgtccctac agaagcagcg tacacgattc aaaatgctac ggcaggggat aaagcactgc
     4201 tgcttgttcc agaagcagaa cacttggaag catcgatgaa agatccgaaa acctattgga
     4261 cagtgatttt ttcatttatt gaacaaagaa taacgatgaa ttaattttct cttctttttt
     4321 gtaagtaatt cgaggataaa taattgttta tagatcagga gactgctaaa ataaggtagg
     4381 aataaaaaaa tatcagttat ttgaggagtg cggcatatgg aaatcaaaga agaaaaaaat
     4441 cgtcttgctt tattcaatga tgaacaacaa gagatcggag aaatgacatg gtcagatgcg
     4501 ggcccagaca tcatgatcat tgaccataca tttgtagatc cagcatatcg tggtcaaaag
     4561 ttagctgaaa agctagtgta tactggagta gaattagcaa gacgagacgg gaaaaagatc
     4621 attcctttat gcccttatgc aaaaaaagag tttgaaaaga aaccagaata tcaagacgtc
     4681 ttacgtcagt cataagaata acagctgaaa gttttgaact ttcagctgtt attccattta
     4741 tatagagttg tcacggactt ttatctatga taaagttaaa gagacgatat cttttgggaa
     4801 ggaggctgcg atatgtataa tttaattttg ggaatcgtga tcgttttatc gattgttatg
     4861 gtcattgcga ttatgatgca gccaagcaag caaaatagtg cagcgagtgc atttaccggc
     4921 ggtgcggatc aactattcgg caaacaaaaa gctcgcggct ttgaagctgt catgcagcga
     4981 tcaactgcag ttatgggagc tatctggatg atcttattat ttgtcctagc atatttatct
     5041 tcgaagtagt tgaataagcc tctcgcaaaa cggcgggcgg ctttttttat tgcaaagaga
     5101 acgcttaaga agaagttatg aaagaaacta tggtaaaatg aggcgaaaga ggtgagacga
     5161 gtgagacaat taccaaaacc cctttatgca aaacatggaa agcgagcggt tttgctgctt
     5221 cacgcttact caggaagtcc aaacgatgtt cgaatgctcg cacgattttt ggaaaaagca
     5281 gattatacag tttatgcgcc gctttttaaa ggacacggaa caatggagcc atatgatatt
     5341 ttgcaagaaa aagcagaaag ctggtgggca gacactaaaa aagccatcca ttttttacag
     5401 tctgagcagt tttctgatat tgccgtatta ggattatcaa tgggcggcat ctttgctgta
     5461 cgagcactag aagaagaatc agtgattggc ggcggttttt tctgttcgcc tttgtctcct
     5521 gtaaaaacaa acgtgcctga aaactttgaa aaatacgtac gtcaagtgct gaagacagca
     5581 ggtaaatcag agaaagaaat caatgaaaaa gcagttgctt accgctcgtt agctgaacaa
     5641 caattaatgg atatacaaga tcaggcagca atcgtagaaa gtaggttgtc agatatccaa
     5701 cagccgatat ttttagctca agctgggaaa gatgaaatga tcgaccctaa tggcgtattt
     5761 gaaactgcac gcaagttgag ccgtcagcga gtgacacttc gatggtatcc tgaaagtggt
     5821 catgtgatta cagttggtac agcaagacgc gaattagaaa aagacgtctt ggaattttta
     5881 gaaaaattac cttggaatga ggaataaata tgacaaaaga aacgataaaa gagaaaattc
     5941 tctttttcat ggaaagcagt cgcaaacgca gtttttctat ggaagaaatc gctgaaggtt
     6001 tagggttcca aaaaagcgat gattttaaat tactagtaca aacgattgct caaatggaaa
     6061 gagaacagag tatagttttc aataaaaaag gaaaagtgaa acttccttta aaaccaatct
     6121 tgatcgaagg aacatttcga gcaaatgaac gaggatttgg atttgtaacg attgatcccg
     6181 aagaagatga catctatatc ccaaaagaag cgactggttt tgcaatggat ggagacaccg
     6241 tagcaatcga tatcatgaag cgagcaaata cagatatgga tcgcggagca gaggggaaag
     6301 tagtcgagat tcgagaccgg gcgactactc agttagcagg ggaattcgtt gcttattcag
     6361 aagaagaaat acaagaaacg gatctttacg gcgttgttat cccgaaagat agaaagctca
     6421 accaatttaa agtatatgca gcagcagagg gagttcgtcc agttgacgga agtattgtgc
     6481 tgatcgaact gacccattac cctgaaaaag ggtatgcgac tagtttagaa gggataatca
     6541 agcaagtgat tggtcataag aatgaccctg gcatggatat cctctcaatt gtcgtggcta
     6601 atggtatccc tacgaaattt cctgatgatg tattgtctga agcagatgaa gttccagatc
     6661 aaatcagcga aaaggatata actggacgta gagatttaag aaatcagctc attgttacga
     6721 ttgatggaga agatgcaaaa gatctagatg atgcagttac ggttaaaaaa ttagataacg
     6781 gtaattattt tcttggagta cacattgcag atgtttctta ctatgtgaca caaggcagtc
     6841 aattggacag agaagcatat gaaagaggga caagtgtata tttgactgat cgagtgattc
     6901 cgatgatccc gcaacgttta tcgaatggta tctgttcact gaatccccat gttccaaggc
     6961 tgaccatgag ttgcgaaatg gagattgatc caaatggaca agtagtctct catgatattt
     7021 tcccaagtgt gatccaaaca acagaacgga tgacctacac agctgtcaat caaatcttag
     7081 aagatcaaga cgaagaaacg atgcaacgct atgctcagct cgtacctatg ttccaagaaa
     7141 tgggtgaatt gcatcgaatc cttgaaacga tgcgaatcaa acgtggcgcg atttcttttg
     7201 aggatcgcga agcaaaaatc cttgttgatg ctgaaggaca cccaaatgat atccagctaa
     7261 gaagcagagg aattggtgaa cgcttgatcg aatctttcat gcttgcggcc aatgaaacgg
     7321 tagctgaaca ttataataaa ttaaatctgc cgtttattta ccgaatccat gaacagccaa
     7381 aagaagaaaa aatgcagcgc ttctttgatt ttgcttcagc tttgggcatt ctggtccgag
     7441 gaacgaaagg aacgatcaca ccaaaagatt tacagcatgt gatcgaagga gtagaagaca
     7501 aaccagaagc tgcagttatc aatactatgt tgctaagaag catgcaacaa gcacgctatt
     7561 cagaagacaa ttttgggcat tatggcttag cggcagatta ctacacacac tttacatctc
     7621 ctattcgacg ctatcccgat ctaatcgtcc atcgtctgat ccgttcttac agtgaagata
     7681 aaagtgaaag caatcaaagt tcgtgggcta cggaacttcc tgaaattgca gatcatagtt
     7741 ctaagatgga acgtcgtgca gtagaaacag aacgtgaagt agattccatg aagaaagcag
     7801 aattcatgat ggacaaagtg ggggaagaat tcgatgggat catcagctct gttgtgaaat
     7861 tcggcctgtt tatcgaactg ccgaatacag tagagggatt gatccatatc aatgaattga
     7921 aacaggacta cttccatttt gttgaaaatc atttagctct tgtaggtgaa agaactggtc
     7981 tgacttttaa aatcggacag aaagtgcgag taaaagtagt caaagctgat ccagatgaac
     8041 gagcagtaga ttttgaactt gtttccgcag aagaggttac agcgttggaa cggccaaaaa
     8101 cacgaaatgg aaaatcagga aaacgcgaag gaaaacaagc aggaaaacgc ccagaaagaa
     8161 atggcaagaa gaacaaacga tatagcgata gtaagcaaac gttcggtaag aaaaaaggga
     8221 agaagccatt ttacaaaggt gttaaaaaga agaaaaagaa aaaataaaat gagttggagg
     8281 aaacagctat gccaaaaggc gagggaaaat taattgcaca aaacaagaaa gctcgccatg
     8341 attattcgat catcgacacg atggaagcag ggatggtctt gcaaggaacc gagatcaagt
     8401 cgatacgaaa cagccggatc aatctaaaag atggatttat tcgcgtccgc aacggagaag
     8461 ctttcttgca taatgttcat atcagtcctt atgaacaagg aaatattttt aatcatgatc
     8521 cgttgcgcac gagaaagtta ttattacaca aaaaacaaat catccggctt gaaaatgaat
     8581 tgaaaaatac tggaatcact gttgttcctt taaaagtcta tattcgtaac ggctatgcca
     8641 aggtattgat tggtctggcg aaagggaaaa aatcttatga taaacgggaa gatttgaaac
     8701 gaaaagatat cgatcgacaa attgatcgaa cattaaaaaa tttctctaga taatttcatt
     8761 atcctttttc gcatattcgg tgcttttgct tttacttaat gataaaattt gttagcattt
     8821 aatctgataa aacatgatta tcataggttt tttcttagat caggagcacc gtgtataaag
     8881 aaaaattcgc ttcgtttttg actgaaataa aaaagtgtct aattttcatg aaaatgtttt
     8941 gaattttctg tttcatggtg gtatgatacc gtgtggttag aaaccgattg gaaaaaacca
     9001 gatacgcaca tgaattgatt ttatgtgaat ttcggggaaa agaggtgaaa tggattggat
     9061 gaacgctcgc taacgttcca cattggacca atttggtttg atggtacagt ttgtataatg
     9121 gtgttactga cctgtttgat tgttttcttc ttagcttact tttttacaag aaatcttaag
     9181 ttgaaaccaa caggcaaaca aaatgcgata gaatgggtca ttgattttag tcgaggaatc
     9241 gtaacagata atctgcctag aaaagaattg agcaatttcc atttgttggc atttacattg
     9301 tttttatttg tttttgttgc aaacaacatc ggtttagtaa caaaaatcgt tttacctaac
     9361 gaaacaacac tttggaaaag cccgacagca gacccgtttg ttactttaac tttggcattt
     9421 ataatgatct tacttactca cttatttggg gtgaaaaagt taggcttcaa aggatatttc
     9481 gtaaattcgt tcttgaaacc atatagtttt atgtttccga tgaaactgat tgaagaattt
     9541 acaaacttat taacacttgc tttacgtctt tatgggaata tttatgcggg ggaagttttg
     9601 ctaaccttga ttgcaaaaat ggtaataagc ttaggctggt tctcattgcc gttagcaatt
     9661 ccattagaga tggtctggat cgcattctcc ttgtttatcg gaagcatcca agcatttgtc
     9721 ttcgttactt tatctatggt ttatatgagc cataagattg aagtggaaga ataatattcg
     9781 ttttaaaaac aaactactat ttatctaatt taggaggaaa acaattatga attatatcgc
     9841 agcagcaatc gcaatcatgg gagcagcaat cggagccggt tatggtaacg gtcaagttat
     9901 ctctaaaaca atcgaatcaa tggctcgcca acctgaaatg tctggtcaat tccgtacaac
     9961 aatgtttatc ggggtagctc tagtcgaagc tgttcctatc ttaggggtag ttgtcgcctt
    10021 gattctagtt tttggtgttt aattgaatca gtattaaggc agaaacaatt tggtttgtta
    10081 ctgctttttt acatgaaaac tagaagtact tcaaagaaag gaaggctcgc ctatgctgaa
    10141 tcaattggca attgcagaag ttggcaatgc gatgttagga aatgtcatcg ttgtcagcgg
    10201 ctcatttttg atactactgg ctctcttgaa gcattttgct tggggaccaa tcagcgatat
    10261 tttgaaaaaa cgtgaagaca agatcgccaa tgatttggat tctgcagaac aatctcgcat
    10321 caactcagcg aaaatggaac aagaacgcga acaacaattg ttagcctctc gttctgatgc
    10381 agctgatatc atcaaaaatg cgaaagaaag tggagaatta agccgccaaa atattttgaa
    10441 ggatgctcaa gaagaagcag ctcgtctaaa aagtaaagcc caagctgata tcactgtaga
    10501 acgtgattca gcgctgaact ctgtaaaaga cgatgttgca gaactatctc ttcaaatcgc
    10561 ggaaaaaatc ttgaacaaag aattatctcc agaaatgcac gaatcattaa tcaatcaata
    10621 tatcgaaggt ctaggttctt caaatgaaac tagataaata tacagtagga agacgttacg
    10681 ggaaagcttt atttgaactg gcgatcgatt caaataaagc agaagaaatt tatcaagatc
    10741 tcgtaagctt acgtcaaatc tatgaacaag tacctggatt aggtaatatg ttaagtgatg
    10801 ttcgtctaga acctcatgaa aaacgagaaa tcatggacaa gcttgttagt ggctatgatg
    10861 gaatcgttaa aaacttttta gaagttgttt atgcgtataa ccgaatggat gatcttttgt
    10921 tcatgatcga agaatatgaa cgtcgatatg atgaacatca aggtttgctg ttgggaagtg
    10981 tcacaacagc agttccactt tcagacgaac aacgaataca attagaaaag aatgttgcaa
    11041 aaacaatgaa ctatcaatca gtcgaattaa aacaaatcgt tgattcttct attattggtg
    11101 gcgcgattgt tgaagctaac catcgagtaa tcgacggaag cattcgcacg caattagaaa
    11161 atatgcgtaa tcaattaaat agatagtaca aagaggtagg tgaatcgaat ggccatcaaa
    11221 gcagaagaaa ttagtgcctt gattaaagaa caaattgaaa attatcaaaa cgtgctttca
    11281 gtcgaagaaa tcggtaccgt aacttatgta ggtgacggga tcgctcgtgc acatggttta
    11341 gaaaacgcga tgagtggtga gttgcttgaa ttttcaaatg gctcatacgg aatggcgcaa
    11401 aacttagaat caaatgatgt aggtatcatt atccttggcg atttcgagtc cattcgtgaa
    11461 ggagataaag taaaacgaac aggaaagatc atggaagttc cagttggaga ggccttgatt
    11521 ggacgggtag ttaatccgct aggtcagcca attgacggac taggtgaaat cgttactgac
    11581 aaagctcgtc cagtagaagc aatggcacca ggcgttatgc aacgtaaatc tgttaacgaa
    11641 ccaatgcaaa caggtctaaa agcaatcgac gctcttgtac caatcggacg cggccaacgt
    11701 gaattagtta tcggtgaccg taaaacaggg aaaacttcca ttgcgatcga tacgatcatc
    11761 aaccaaaaag gtcaagatat gatctgtatc tatgtagcaa tcggacaaaa agattctaca
    11821 gttcgtacac aagttgaaac attgaaaaaa tatggcgcaa tggattacac aatcgttgtg
    11881 aatgccggtg cgtctcaacc agcgccgttg ctttatatcg caccatatgc tggtactgca
    11941 atgggtgaag agttcatgta caacgggaaa catgtcttga tcatttttga tgatctttca
    12001 aaacaagctg ttgcttatcg tgaactttcc ttgctgcttc gccgtccacc aggtcgtgaa
    12061 gcttatccag gggatgtttt ctacttacac tcacgcttat tggaacgtgc agcaaaatta
    12121 agtgatgatt taggtggcgg ttcaatgact gccttaccat ttgttgaaac acaagctgga
    12181 gatatctctg cttatatccc aacaaacgtt atttcgatca ctgatggaca aatcttctta
    12241 gaaagcgact tgttctacgc aggtactcgt ccagcggttg atgccggact atctgtatct
    12301 cgtgtagggg gttctgcgca aatcaaagca atgaaaaaag ttgctgggac tttacgtcta
    12361 gacttagcaa gttaccgtga attagaagca tttacacaat tcggttctga cttggatgca
    12421 gcaacacaag caaaactaaa tcgtggacgc cgtacagtag aaatcttgaa acaaaaattg
    12481 catgcaccgc tagctgttga aaaacaagta gtgattttgt atgctttgac acatggattc
    12541 ttagatagta tccctgtaga cagtatttta gacttcgaac atgaattatt cgaatatctg
    12601 gataccaatc attcagatat ctttgaaaca atccgtacaa caaaagatct tccagaagaa
    12661 gaagcgttga acagtgcgat tcaggaatat aaagacatgt tcttagctac aaaaggaaat
    12721 aattcctcta cagaagataa attgaaatcc attcaaaatg catagtgagg tgagatgaat
    12781 gggtgcttca ttaaatgaaa tcaagacacg tatcgcttca acgaaaaaaa cgagccaaat
    12841 cacgcgggca atgcagatgg tgtctgcttc taagttgaca aaatctgaag cttcttcaca
    12901 gaagtttcag gtttatgcaa caaaagtaag agaaatcgtt acgcatttga ctgctactca
    12961 gttaaatgat attgcctcag aaaatcctag aggggatatc aattacaaca gcatgttgct
    13021 cagccgtcct gttaagaaaa cgggttatat cgtcatcact gcagatggcg gtttagtcgg
    13081 tggatataac agctcgatat tgaaacaaac gatgtctatt ttagaagaag accaccaatc
    13141 acccgatgat tacgtgatga tcgcaattgg cggaactggt gcagacttct ttaaagcacg
    13201 cggtatcaat ctagcatatg agctgcgcaa tctatctgat cagccaagct ttgatgaagt
    13261 ccgcaaaatc gtaaatatgg caacaactat gtaccaaaac gaagtattcg atgaattata
    13321 cgtatgttat aaccatcata tcaattcgct aactagtcag tttcgtgtag aaaaaatgct
    13381 gccaatctca gacttagatc cagaagaagc aactactttc gaacaagaat atatttttga
    13441 accttcaaaa gaagaaatct tgtctcagtt attgcctcag tatgctgaaa gtttgatcta
    13501 cggtgcgatc gttgacgcca aaacagcaga acatgctgct ggtatgacag caatgaaaac
    13561 agcaactgat aatgctgcta atatcattga tgagctgacg gtttcttaca accgtgcaag
    13621 acaaggggct attacccaag aaattaccga aattgttgcc ggagcttcgg cactagaata
    13681 gtattgagat tggaggaaaa aagatgagtt caggcaagat tgttcaagta atcggtcccg
    13741 ttgtcgacgt ggaattttct ttagaccaat ccttaccaga tatcaacaac gcattagttg
    13801 tttataaaaa tgacgaaaat aaatcaaaag ttgttcttga agcagcgtta gaattagggg
    13861 acggagtgat ccggaccatt gcgatggaat caacagatgg tttacaaaga ggaatggaag
    13921 tcattgatac aggcaaagca atctctgttc ctgtaggtaa agaaacatta ggtcgcgtat
    13981 tcaacgtact aggagatacg atcgatttag aaacaccttt cccagaagat gcggaaagaa
    14041 gcgaaattca taaaaaagca ccagcctttg acgaattaag tacaagtaca gaaattttgg
    14101 aaacagggat caaagttatc gatttgcttg ccccatattt aaaaggtggg aaagtcggac
    14161 tattcggtgg tgccggtgtt ggtaaaaccg tactgatcca agaactgatc cataatatcg
    14221 cccaagaaca tggtggtatt tctgtattta ccggtgtagg tgaacgtact cgtgaaggta
    14281 atgacttgta ttatgaaatg aaagattcag gagttatcga aaaaacagcc atggtgttcg
    14341 gacaaatgaa cgaaccacca ggtgcacgta tgcgtgttgc tttgactggt ttgacgattg
    14401 cggaatattt ccgtgatgta gaaggtcaag atgtactgtt gtttatcgac aacattttcc
    14461 gtttcactca agctggatct gaagtatcag ccttgttagg acggatgcct tctgcggttg
    14521 gttatcaacc aacattggca acagaaatgg gtcaattgca agaacgtatc acatctacga
    14581 aaaaaggttc gatcacatcg atccaagcaa tctatgtgcc agccgatgac tacactgacc
    14641 cggcgccggc aacagcattc gcccatttgg atgcgacaac caacttggaa cgtaaattaa
    14701 cagaacaagg gatttatcca gcggtagatc cattggcttc ttcttcaagt gctttagcac
    14761 ctgaaatcgt gggagaagaa cattataaag tagcaacaga agtccaacac gtattgcaac
    14821 gatatcgtga attacaagat atcattgcta tcttaggtat ggatgaatta tccgatcaag
    14881 aaaaagtgtt agtttctcgt gcccgcagag tccaattctt cttgtcacaa aactttaacg
    14941 tggctgaaca atttactggt cttccaggtt catatgtccc tgtagaagaa acagtgaaag
    15001 gattccgcga aatcttggaa ggcaaatacg atgatcttcc tgaagaagct ttccgaagtg
    15061 ttggccgaat cgaagacgtc gtagaaaaag cgaaaacact aggctactag aaaggggtgc
    15121 cccatggatc aatatttaac agtaaatgtg gtaactcctg ccggtttggt ctatgatcac
    15181 catgcagcta tcgtcgttgc acgtacgaca gctggtgagc ttgggatttt gcctaaacat
    15241 gcaccaatca tcgttccatt gacgattgat gaagttcgtg taaaacgaac agattcagat
    15301 acccacgtcg attggatcgc tgttaatggc ggaatcatgg aagttcgtga taatgttgtt
    15361 tcaatcgttg ctgatagtgc ggaacgtgaa cgtgatatcg atgtatctcg tgcggaacgt
    15421 gcaaaacaac gcgcagaacg ccaaatcgaa gaagcaaaag caaaacaaga tacgaacgaa
    15481 ttgaaacggg caactgttgc gttgcaccga gcaatcaatc gtatcaaagt atctaagcac
    15541 tcataaaatg agatgcggat gtttatccgt gtttaagaac agtaaaaaga ggttatgaca
    15601 gaagtggtca gcttcaagac ataagaagga atttacgaaa attgcttctc aaatttttgt
    15661 gaaatctcag cttatttttc gaagaagcta cttctggaac accgtatatc ggaggttgtg
    15721 aaatcagcgt tttgtcctga gtattagaga aacaaatgga aaatagttcc tcatattttg
    15781 ccgttttttg agctaatacc gagggtcaga tgattgaaca ccgtttattt ggaataaggg
    15841 actgcgacaa gac
//
LOCUS       DACEXQ010000035        15709 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.035, whole genome shotgun sequence.
ACCESSION   DACEXQ010000035 DACEXQ010000000
VERSION     DACEXQ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15709
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.035"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..599
                     /locus_tag="IXH04_000890"
     CDS             <1..599
                     /locus_tag="IXH04_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAQ1851519.1"
                     /translation="SPIHLTKGEETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQL
                     SYVEFKKERYYLTDIHFHLPSEHIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQE
                     GWLYNQENGESWNLEKHEHWVNPDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVG
                     KLNQEFLLPFDGQYARPNNRPIQPLNGREIYYFEEFEQ"
     gene            596..1498
                     /locus_tag="IXH04_000891"
     CDS             596..1498
                     /locus_tag="IXH04_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1851520.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(1656..3362)
                     /locus_tag="IXH04_000892"
     CDS             complement(1656..3362)
                     /locus_tag="IXH04_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAQ1851521.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            3552..4175
                     /locus_tag="IXH04_000893"
     CDS             3552..4175
                     /locus_tag="IXH04_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851522.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            4625..5674
                     /locus_tag="IXH04_000894"
     CDS             4625..5674
                     /locus_tag="IXH04_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1851523.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            5671..6552
                     /locus_tag="IXH04_000895"
     CDS             5671..6552
                     /locus_tag="IXH04_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1851524.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            6555..7325
                     /locus_tag="IXH04_000896"
     CDS             6555..7325
                     /locus_tag="IXH04_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1851525.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            7604..8458
                     /gene="aroE"
                     /locus_tag="IXH04_000897"
     CDS             7604..8458
                     /gene="aroE"
                     /locus_tag="IXH04_000897"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAQ1851526.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            8636..9799
                     /locus_tag="IXH04_000898"
     CDS             8636..9799
                     /locus_tag="IXH04_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAQ1851527.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            9916..10326
                     /locus_tag="IXH04_000899"
     CDS             9916..10326
                     /locus_tag="IXH04_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ1851528.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            10500..12728
                     /gene="topB"
                     /locus_tag="IXH04_000900"
     CDS             10500..12728
                     /gene="topB"
                     /locus_tag="IXH04_000900"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ1851529.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            12866..13183
                     /locus_tag="IXH04_000901"
     CDS             12866..13183
                     /locus_tag="IXH04_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851530.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            13343..13579
                     /locus_tag="IXH04_000902"
     CDS             13343..13579
                     /locus_tag="IXH04_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1851531.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            13576..13974
                     /locus_tag="IXH04_000903"
     CDS             13576..13974
                     /locus_tag="IXH04_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAQ1851532.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(14641..15585)
                     /locus_tag="IXH04_000904"
     CDS             complement(14641..15585)
                     /locus_tag="IXH04_000904"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAQ1851533.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
BASE COUNT         5224 a         2702 c         3206 g         4577 t
ORIGIN      
        1 agtcgcctat tcatttgacc aagggggaag aaaccaaaat agaaggagac acgctttcct
       61 ttcattatca aaagcaacgg tttactgaca aggaattcaa aaacacgatt catttagtac
      121 cttttgacca actcagttat gtagagttca agaaagaacg ctattatttg acagatattc
      181 atttccactt gccttctgaa catatcatca acggaaaaca ggaagatatt gaatttcatt
      241 ttgttcatat gaacagcaaa aaagagaatc tagttgtggg tgtcatgtac cagctaatgg
      301 tacaggaagg ttggctgtat aatcaagaaa atggggaaag ttggaactta gaaaaacatg
      361 agcattgggt aaatccggat gtgttctttc cggagcaaaa aagccatttc cattatattg
      421 gctcattgac aactccgcct accagcgggc caatccaatg gtttgtaatg gatcatgtag
      481 gaaaactgaa ccaagagttt cttctcccct tcgatggtca gtatgctcga ccgaacaacc
      541 gaccaatcca gcccttgaat ggaagagaaa tttattattt cgaggagttt gagcaatgaa
      601 tattcagcag atgaaatacg tggtcgctgt agcaaacaat ggtagtttca gagaagcggc
      661 aaagaaaatg tttgtctccc agccaagtct ttcagctggt atcaaagaat tagaaaccga
      721 gttagggatc acgctgttta ctcgaacgaa tcgaggggca tatttaacag aagaagggca
      781 agacttctta aatcgtgccg agaaaatatt ggtccagctt gatacattag aaactcacta
      841 tctaacgagt gaaaagagag acaatttttc gatcgctgcc cagcattatg atttcttagg
      901 tcctttgacc gcacagctac tcgaggaatt caaagacgag atcaaacaat ttcggatcat
      961 ggagacgaca acagcgagag tgatcgaaga agtcaaagag cagcacagtg agatcggcat
     1021 tatttatatg aatgagcata atcaagctgg tatcagaaga tatttacaac aaggagagct
     1081 aagtggccgc agcattggaa aattcaaaac gcatattttt ttaaggcaag gacatccatt
     1141 agctgaacac gcagagatca ccaaagaaga attgttatct tatcctcaag ttcgttttac
     1201 acaggatgga aataatttcc cgtattttta tgaagacctt atcgagatcc ctgatcaaga
     1261 aacagtgatt tatacgagtg atcgaggtac tttgatgaat atcgttcttg aaactgatgc
     1321 ttacgcttca ggatctggta ttgtgattgg agggattcgc gagcacttgc ggttaatccc
     1381 tttggcagaa ggcgaactaa atgaatttta tatcatttat tcggcgaaac gtccgctcag
     1441 tgaaatcgct caaagattta tcaaagagct tactcggtta ctggaccagc agagctaaga
     1501 aaagcagatg agaacgctgt ttattgaaca ccgttgatca aaaaagaggg gttgtgacag
     1561 aattttgtca caactcctct ttttgtagca aacaatttat aactaattgc tgttgtggga
     1621 aagtacgcaa atatgattct agaaacactc attgattacc tgttttgttt catagctgta
     1681 attttttctt cttctggtgt cacgataatc gtcttttggt tttcataaat aacataacca
     1741 ggcttggcgc catttggttt tcggatatgc tttacctgta caagatcaac cggcacttgg
     1801 gcagagtatc ggtattttga aaaataggca gctaactcgg cagcttctgt aattgtttca
     1861 tctgagggct tgtcactttt aatgatgacg tgcgaaccgg gtatattttt tgcatgaagc
     1921 caatagtcgg tttttttagc tgtcttcatt gtcagctggt cattttgaag attgttttta
     1981 ccaacaagaa tgagcgttcc gtcagaagaa aagtattgat caggctgaga tggtttctta
     2041 cgtttttgtt tttttaagtt cttctttttc aggtatcctt ctgaagtcaa ttcttcttta
     2101 attgcttcga tatccattgg gccggcaatt tccagttgag ataagacaga ttcaagatat
     2161 tggatttcat ctttggcttc ttggatttgt tcgccaatca gcttaacagc gttcttcagc
     2221 ttttggtatc gatggaaata tttctgtgca ttttgatttg gtgtcaatgc cggatctagt
     2281 gctattttga ttggccgatc ttcttcataa taattaggta aaacaacttc ttttgcgccg
     2341 cgtggtactt gtgtcagaaa agtagtaagt agttcgccat ttctacggta attctcagcg
     2401 ttttcggatt ctttgagtgt ttgttcacgt tttttcaatt tatttttatt tcgttttagt
     2461 tcattttcga tttttcgaat cagttctccg ccttgttgtt ttgcccgatc tttctctgct
     2521 ttttcgtgat aatagctatc taataattga ctgagtgttg gataagctgt tacagcagat
     2581 gcctgttcgg acaatacctg ataagcaatc ggtgtgaaaa aatctttatt ttccgtttcg
     2641 taaaaagtag gaataggctg atggctgatg gctgagaaaa attcgtccca tacaaccatc
     2701 ttttcattag gacgttccag taatcttttg gtcagctctt gagcgatatc gaaaccgatt
     2761 ccctgaaact gacgttggat tgcttttgga tctagttcag tttgggaaag ccgctgaaag
     2821 attttttctt tctcggagct aaatgggttc aagacctctt gttttggcgg agcaacatat
     2881 tctacaccag gaagcaaaga acgataagtg ttttgagagc ttccaatgtg tttgatcgca
     2941 tccagtattt ttccagtttc tcgattaact aagacgatcg tactatgacg tcccattaat
     3001 tcaacgatca atatgatatt ttgtagatca cccaactcgt ttcgtttagc aaagtggaaa
     3061 tggatcactc gatcattttc gatttgttcg atgtcttcta aaatagcgcc atccaaatat
     3121 tttcgaagca tcatcacgaa gttaggcgga ttatcaggat tctgataatc gatttgggta
     3181 atctgtacac gagcataact tgggtgcgca gataataata atcgatggtt tttgccgcgt
     3241 gaacgaatca ccagcacgat ttcattttca taaggctgat ggattttcga gatccggcca
     3301 gatagtaacg tttcacgcaa ttcgttgacc atcgcgtggg taaatacacc atcaaaggac
     3361 atggttcatt cctcatttct tttcataagt atattcatta taacgaaaaa aagaagaaaa
     3421 accaatttgg ccgaatagta aagcaaaagt ctagaaaaat cttggactaa ttacataaaa
     3481 aacagtgttt ctatcgttga aatccaaatt tttaggtata ataaaaaata ggagaaaaag
     3541 tagaaaagaa ggtgtttgtt atttgggaag tagttaaatc gagagtacaa gaaatgaatg
     3601 aacatttctc agtgtcattc gcagaaaatc caagtaagta ttttcgacgt cttgtttttc
     3661 tgctcatctt atcattgctg ttaacagttg caatgttttt attgccggaa aaaatccgag
     3721 aagagcaagt aaatagtctc gtttattcgt ccataccaac cagcaaattg aaaccttttg
     3781 attacagcaa agctgatcag aaaatcaaac aaagccgggc gatatcggtg atgttcagca
     3841 aaccgaacgg gaaaacctat caggacatgc ttgatgtttt taatgatcct gagcttatgg
     3901 aagaattgaa tcgaccgatc ttttattatc cgatcgttta tgatgtacat gaactggaac
     3961 aaaaatataa catacgaacg gatgaagtga catttatttt ctttgatgga ggaaaagaag
     4021 caaatcggat aactgcgggt aagggaggaa taactgattt tgataaggag ttgatccctg
     4081 aactgaatcg acttccgtta gccaatatca aacaattaga agaagaattg gatagaacgg
     4141 gagtaacttc ccaaaattcc gagattgcta attgagacat tggcgcgatg aaacggtggg
     4201 tctatatagc gctattttta attatcagaa caagtgatgt ttgatcttca aaataacaaa
     4261 taaaaaatta aaaaatatag ttgacgaaat tcaaaaattc gattagactt aaatcaattc
     4321 ttaaggaaag ggtgacctgt aatgaactta tcaaaacgta tttcagcaga aaacaaaaaa
     4381 aattggcgca gctggcgtag ataagttgta ttaatggtga gactttccca tagatacaac
     4441 tttttagagt gcatttctaa agcttgtatc tatgctagac atgaacattg actgccgcat
     4501 agattagaat ctatgcggtt ttttgtgtaa agacaatgac cgctttggcc attgtctttt
     4561 tttgtagact ggatacttgc ctgattataa taaataaaaa gattgacttt gcaggaggaa
     4621 aaaaatgaaa aacaaacgat taataacagt tgtagtaatt attttagttt atttagcagg
     4681 tgcatttatt tatggaaaag cctcaggcga aaaagaaagt acttcgacga accagacaaa
     4741 aaaaacaaca gttgttgggg tattgcaata cgtgagccat ccggcattag atgagattta
     4801 tcgaggaatc aaagatggtc tgaaagaaga aggattaaaa gaaggagaaa atcttgagat
     4861 caaatttcaa aatggacaag ccgatcagag caagctagcc acaatgagtc aacagctagt
     4921 acaatctgat ccagatgcct tgattggaat cgcaacacct gccgctcaat cattagcaaa
     4981 cacgacttca tcgatcccag ttgtgttagg tgctgtaacg gatcctgtgg gggctggatt
     5041 agtcgcttct ttagagaaac ctggtggtaa tgtaacaggt gtttctgatc agccgcctgt
     5101 tacttcacaa atcaagcttg cttctgattt gttgccaaat gcaaaaaaag tcggaatctt
     5161 gtatgcttct tcagaggata attcaaaata ccaagtagca caagcagaag aagctgcaaa
     5221 aaaagaaggg ttagaatcag taaaatatgc ggtgccttcc actaatgaga ttgcacaaac
     5281 ggttcaagtg atgagccgac aagtcgattt tatctatgtt ccattagata ataccattgc
     5341 caatgcgatg caaacagtgg taaaagaagc aaacaaagca aatatccccg tcattccatc
     5401 tgtggataca atggtggaac aaggtggact agcaacaatc gggatcaatc aatatcaatt
     5461 agggcttcaa agcggtaaga tggcggcaga acttgcgagt ggaaaagaaa aaccggaaac
     5521 gacgcctgtt tatacatttg atcaagggga tacagtgatc aatcaatctc aagctgatcg
     5581 cttaggaatc attgttcccc aatcgatgaa ggaaaaagcc aaaatcatta cagatgactc
     5641 gcagcaagaa acatctaagg aggatgacaa atgatcgtat cagctatttc acaaggcatg
     5701 ctttgggcaa tattaggatt agggattttc atgacttatc ggatcttaga ctttcctgat
     5761 atgacgacag aagggtcctt cccattaggt gctgctgtct gtgtgacagc tattactcat
     5821 ggggtagcgc cgattgtagc tacattatta ggcgtaggtg ctggcatgat agcaggactc
     5881 gttacggggt tactttatac aaaaggaaag ataccggtta ttttagcagg gattttagtg
     5941 atgtcaggat tgaactcagt tattttgttt gtgatgcgaa caccaaatct ttctttattg
     6001 aatcatccga ttctacaaga cagcttccat cgctttgggt tacctgatta ctttgacaca
     6061 gtccttcttg gcttcatctc tttgggcatc ttgattgctg tactgctatt cttcttcaat
     6121 acagatttgg gtcaaggcta tattgcgaca ggagacaacg aaacgatggc tcggtcgtta
     6181 gggataaaaa ccgatcgtat gaaaatttta ggtttaactt tgtcgaacgg tgtcattgca
     6241 ttatccggtg cgctgattgc ccagaacgac ggatatgcag atgtaaataa agggattggc
     6301 gtaatcgtca tcggtcttgc ttccatcatc atcggtgaag tgatcttcca agaattgacg
     6361 ctagcagaaa gattgattgc tattgttgtc ggcagtatca tttatcagct attgatttta
     6421 gtagtaatca aactagggtt tgacacgact tatctgaagt tgttctccgc tatcatctta
     6481 gctatttgtc tgatgatccc gcaattgaag aaagcactga acttaaaaac gggttttgag
     6541 aaggaggcat agtaatgact gatatattga caatcaaaaa tggcagaaaa gtcgtaaaca
     6601 atggaatgaa tgaagagaaa gtactattgg atgaaattaa ccttacaatc agagaagggg
     6661 acttcatcac tgtccttggc gggaatggtg ctggaaaaag cacactgttc aatacgatcg
     6721 ctggtacgct tcaactcagt caaggatcga tctcttttaa agatcagctg atcacaaaag
     6781 attcagaaga aaaaagagca cgattccttt ctcgagtttt ccaagacccc aagatgggaa
     6841 cggctcctcg gatgacagta gcagaaaatt tgctacttgc acaaaaaaga ggtcaaaaac
     6901 ggaaattccg tttacgtaac ttgaaaaatc aaaaagaaga ttttttcaga ctatgtcaag
     6961 aagtcggtaa tggcctagag caacacttgg atactccagc gggtgaatta tctggtggtc
     7021 aacgccaagc attaagcttg ctgatggcga cgatcacagc tcctgagtta ctattattag
     7081 atgaacatac tgcagcttta gatcctaaaa cagctaaagc attaatgcga ttaacaaatc
     7141 agcggatcaa tgaacaaaaa ttgacttgtc tcatgatcac gcatcgaatg gaagacgcct
     7201 tgaaatacgg aaatcgtctg atcgtactcc aaaaagggaa aatcgttcaa gatatttctg
     7261 aaaaagaaaa gcaacatttg acaatgggcg accttttgca gttctttgaa gaagtagtgg
     7321 attgatttcg acagaaaaat tcctttcagt agaaggactt gaaaactcat tgacatcttc
     7381 ctttgattca tcctatactt aaaacaataa acaattaaac atatttgggt gttttgtagg
     7441 atcacgtagt caatgatttt aacaattaaa tagaatattc tttgctctga caagtaaacg
     7501 atgagaaaag aatttgctac tttgccacct agacgtttcg tactttttcg tatcaaacgc
     7561 taagtggctt tttttattga agtaagaaca aaggaggaat aagatgattt ctggacaaac
     7621 caaattagct gggctctttg ctgatccggc ttctcacagt ttatccccat tgatgcacaa
     7681 tactgctttt gaagcaaaca acatcgatgc ggtgtattta gctttccaag taaatccaat
     7741 gaatttgaag caagcagtcg aaagtattcg aacttttgat atgttaggtg taaatctttc
     7801 gatgccgaat aaaacagcgg ttattcctta tctggatgag gtaagccagg aagcgcaatt
     7861 gatcggagca gtgaatacga tcgtgcacga aaagggtcgt ttgattggct ataatactga
     7921 tggtgcgggc ttcatgcaat ccgtgcaaga agctgggatt tcgatcaaaa agaaaaaaat
     7981 gactctactg ggagctggag gtgcagcaaa ggcagtcact gttcaagcag cgttagacgg
     8041 cgctgaggaa atcgtagttt ataaacgaaa caaccaaaca ttcgctcaag taaaagaaac
     8101 attcgaacaa ctcagctatc gaacttcttg taagataaaa gttcgtgact ttgccgatac
     8161 gcatcaatta agaaaagact tgaaagaaag ctgtcttcta gtcaatgcaa cagatatcgg
     8221 gatgggaaag aaaatcaatc agtcaccgct cttagatata agcatgatgc atgagaatct
     8281 agcggttttt gatttgattt atgcacctag agaaacacgt ttattaaaag aagcaaaaaa
     8341 agtaggagca aaaggataca atggacttgg catgttgatc taccaaggag caatcgcgtt
     8401 tgaattatgg acaaaggaaa agatgccgat ccatgtgatt gaaaagctgt ttgattaaaa
     8461 aaggataata gataaagatc ttttattctc gcataaacgg tctcaagaaa agtaatcctt
     8521 tatgtatcga agcatttatt tcgttatttt ttattaaatt atcaaaaaat gataggcttt
     8581 tgtcgattgg ttatcgtatc ctttatttat cagtaagaaa cgaggaggaa ccacaatgag
     8641 tcggatggat cgctataagg acatccataa gaaggcgaaa cctctgaagc gggatacggg
     8701 gagtattttt gctagaagag aacgaaaacg aacgcgtatg gaaaatgagg aagatgctac
     8761 ccgtatttat gaaagagagc agcattcatt tgaacatttg aaccaatacg agcaacagat
     8821 atacagagat gcaccaagag cagaagaaca acaaaaaaat aaaaaatttt tcaatcggca
     8881 aaagaagaac aaaccgttaa aacctcgaag aaagcgatcg tggaaaaaaa tcattctatc
     8941 gattttgctt gttttgatcc tttattcagg tatctcattt tttattggta aatatgtagc
     9001 tgaacatgac acaagcttag aggcagcgac ttcagaaacc tttcatgggc aaaaagcgac
     9061 gaatggtgcg cataatattt tgatccttgg aagtgataca cgcggagaag atgccgggcg
     9121 agccgatacg atcatggtct tacagttgga tggtccttcc cataagccga aactgatttc
     9181 ttttatgcgt gacagcttcg ttacgatccc aggggtagga caaaataaga tcaattcagc
     9241 ttatgcatac ggaggagccg atctagtgcg tcaaacttta gtagaaaatt ttggaatcga
     9301 ttgtcaatac tatgcgaaag tcgatttcaa atcgtttgaa aaagtgatcg atgccttatt
     9361 catgaacgga gtgaagatcg atgcagaaaa agatctgaat ctggatggtg tagatattaa
     9421 aaaagggggc cagaagatgg atggacatgt tcttttgcag tacgcacgtt tccgaatgga
     9481 tgaacaagga gactttggcc gtgtcagacg ccaacagcaa gtaatgaacg ctattttcag
     9541 ccaattgaag aatccattga atctgatccg tgcaccttat gcagctggta aagctttagg
     9601 atatacatcc acagatgtca ctgcttggtt cttgacgaaa aacctctttt cgattgcacg
     9661 aggagctggt ggcattgatc gaataagtgt gccagtagac ggttcgtgga attatgggca
     9721 aagtgattat gctggcagta ttcttgaaat tgataatcaa atgaatcaat ctgctattac
     9781 agattttttg aataaatgat cataaaaaag atcaagaaga agctgcgcaa ggcttttgtc
     9841 atgaatcaat caaacgattc actcgtaaaa aacgctttct tatggtagat ttaaccaaga
     9901 gaggagctgt ttacattgga tatctatctg aacttcaaaa atgagtcgcg tgaagccatc
     9961 gctttttatg aaaaagtttt tcagactgaa gcatcaggta tcaccacata cggtgaaatg
    10021 cctggaatag aacaaatgaa cttgccagaa gaagcaaaaa atctaatcac gaatgcacgg
    10081 ctgaacattt atgatacatt agttatgttt gcagatacgc cagaaggaat tggtcctgaa
    10141 ttagttgtgg gaaataatgt ttctttagtc atccagacaa gaaatgaaga agaaacgaag
    10201 cgcctatttg aaagcttagc tgagggtggt aagattgaga tgccactgga tgctgttccg
    10261 tgggctcagt tgtatgggat ggtaactgat cgatttggca tcaactggca gattaaccaa
    10321 gagtgacagc taaagatgaa tattttctgg aaacatcaag cagcaaacgg atcatcgttt
    10381 gtttgcttga tgtttttgtg tctatttcga ctatacggta tctttttttt attgccagat
    10441 gcgaaaatat gttccataat agagaagtcc gcaaaaaatt aagtaacgga ggtcagaaga
    10501 tgaagacggt gatactagct gaaaaacctt cacaagcaaa agcctatgcc gaaaccttcc
    10561 aacagcaaaa gcggcatgaa gggtattatg aaatcagcga tccattgttt gctggtgaag
    10621 taaccatcac ttatggtttt ggacatttgg tagatatggt tgctccagga gcttatgaag
    10681 agcgttgggc aaaatggtct ttagaaaatt tgcctatttt cccagaaacc ttccgttacg
    10741 aagttccaat ggacaaacgt gcacaatttt cgatcgtaaa acgagaattg cagtcagccg
    10801 ataccatcat tattgcaaca gatggtgatc gagaaggtga agccatcgcg tggtcgatta
    10861 ttcgacaagc aaacgcattt tctaaggaaa agaactattt acgtttatgg atcaattctc
    10921 ttgaaaaaga agcaatctat gaaggtttta agaatctcca acctggaaag aactattttc
    10981 caaaatataa agaagcgcaa gcaaggcaga acgctgattg gctgattgga atgaatggaa
    11041 gtccattata tagcttgcta cttcaacgaa aagggattga tggcagtttt tctcttggtc
    11101 gtgtacaaac accgacactc tatatgattt atcagctgca agaagaaatc aaacacttca
    11161 aaaaagaaac gtactttgaa ggagaaggtt taattacgac aaacaaaggg cagttttctg
    11221 gaaaaatcgt accaaaaaaa gcatttaaaa cccaagaaga gttaacgaag gaaatagaaa
    11281 aacttggagc gcatttaggt aagcaaaacg gacggattct tgaagttacg aaaaaggaaa
    11341 agaggttgca tagtccacgt ttattttcgc tatctagttt gcagacaaag atgaatcagc
    11401 taatgaaagc aagtgccaaa gctacacttg atgctgctca aggattgtac gaaagcaagt
    11461 ttctaagcta tccaagaacg gattcctttt acattacaga aaatgagcat cagtatctgg
    11521 ttaaacatct gcaaaattat aaaaaatttt taggaataga agaagtacag acaactgaga
    11581 tcaaagcaaa gaaacgatat gtcgatgcga aaaaagttca agaacatcat gcaattattc
    11641 cgacaaagac gattccgact cctgcacgct ttgcgaagct ttccaaatta cagcaagcaa
    11701 tctatttgca agttttgcgc actactgtgg ctatgtttgc tgcagattat atctacgaag
    11761 agacgaccgt aatgacgggc gtacagcaat taaagctaaa aagcatcgga aagatcccat
    11821 tacagcaagg ttggcaggaa atcttacgca tcaagtcaaa aaagaaagaa gctcagactc
    11881 ttcctcctat cgtagaaaat gaagaagcag aagtagaact gaaaagtatg gagaaagaaa
    11941 cacaacctcc aaaaccctat accgaaggaa cgttgatcac agcaatgaaa actgcgggaa
    12001 aaacgctgga taatgatgaa gcgcaagaaa ttctcaaaga agtagaagga atagggacag
    12061 aagctacacg agccaatatc attgaaaatc ttaagcagaa gcattacatc gaagtcaata
    12121 aaaacgaaat aactgttacg ccaaaaggaa tcactctttg taaggcagta gccaatgaac
    12181 cattactgac gagcgcagaa atgactgcgc gctgggaagg gtacttgaga aaaattggta
    12241 aacaagaggg gacaccatct gtttttttag agaatatcaa aaaattcatc ctacatttat
    12301 tggcagaagt tcctaatcaa atcgacaagg tagacttttc agatgagatt agcgggacac
    12361 gtcagataaa agcgatggag aagaaaaacg atcaattagg aagatgtcca aaatgtaaaa
    12421 agggaatagt catgctttat cctaaaatcg ctacatgctt gaatccggaa tgcgacttca
    12481 aattgtggcc aacagtagct aaaaagaagc tgactaaaac gaatctcaga gatttgcttt
    12541 caaaaggaaa gacaagtaaa gttgtcaaag gttttactgg taaaaaaggg aaattcgatg
    12601 cagttttagt attaaaagaa gatatgacaa tcggtttttc ttttccatgg atcgaaaaca
    12661 gtggaacaaa agcagttgat ggaaacaaag aaacatcaag aaaaaataat gaaaacgaag
    12721 tagagtaaat gaaaacaatg aaatagcgga atatttattc cgaaaagtga gaatataaat
    12781 accaataaaa ctaaaaaata tcagtgaatc aagcaaaaac gttggggtag gctgatttta
    12841 tatagatagc aaggagtttt tttatttgaa aaaagtgcat tcatttttaa attatcttat
    12901 gggagcaatc acgattgtgg ctgtgtacca agcatttcta aagtatcctg attacgggac
    12961 gaagttgttg tcattgacgc acaatcaaac agcatattca ccaacgttga ttttatcgac
    13021 tttgactgca ggagctaaaa gcgctactta tttttttcat aaaacgacaa gcatgctaaa
    13081 gcgaaagaaa cagcgaaaac aattgttggt ggtggtagcg gctatgatca ttatttattt
    13141 tctaccagtt attacacaag tatttcatca atattttggt taagaatacg agagtttgtg
    13201 acaaaagtca tgtcacaaac ttttttcttt gtcatttttt cgtaagaaaa agcaaaataa
    13261 aagtatatac attgattaaa agtatatact tggatataat gtacttacta tagaatgaga
    13321 atggtataga gggtggcgaa atatgaaaaa aatgaaaaag tataaaatta gaaaatctgg
    13381 caatagcgat gtaacaacta tcccaccaga agtaaaagaa ttcatgggtg tgcagacagg
    13441 tgacgcaatc agttatgttt ttcaatctga tggttctgta cgaatgatca aagcacaaga
    13501 agaacctgat attgattcat tagttgattc gatcatgaat caatatgaag acgcgttaaa
    13561 agatttggtt gatttatgag gtatctttcg actaaagaaa ttatcaaaat caacgcgaaa
    13621 gttattttac gtcactcgcc aggcgaaatg atcggtgtaa aagatgcaaa tgcattagat
    13681 atggccgtca aacagccttc acaagcagta ttcaatcaag aactctatcc tgaaatctat
    13741 gaaaaagcgg cgattttagc aattaattta gctaaaaaac atccttttca taatggcaat
    13801 aaacgaactg ctcttgttgc catgttgttg tttcttcaat tgaataattg tccagttgct
    13861 ttttctcgtc aagaagctgt tgattttatc ataatgatta ccactagttc attggatttc
    13921 gattcactaa agtccaagat aaccagttat ttacgacaaa ccgcaataaa atgaacgaat
    13981 taaaaacttt atttctcaat tagatcagca tgtcattaag gaatatgctg atttttttat
    14041 gtttaatcat acaaaatagt tttttcataa aaatatggaa aatatggatt aattttgaaa
    14101 taattgattt aaaaggaata caaaagagtg aatagtagga aatagtggtt tagaactagt
    14161 gggctgattt tttatatatt ttagtttaca taatatatat tatacaaagt agatttaaaa
    14221 aatgataaac ctctctcgaa cggtttttta tcaaaaatcg tcatttttcc atcaaaatct
    14281 gcttatgcaa aattataaaa aaattttgca gtttttctta ctttttttgt cgtttaattg
    14341 aatgataaaa accagtttct ttattcagtt aatcaaatat gaacgaatta gatatgaatc
    14401 attgtatttg ccgttgcgga aggatttttt aattaaccac agataaacat tgataacgat
    14461 tcgcttattg taatcaacag attaaatttc atatgttcat atgtacggat acattttcgg
    14521 accgattaat tttaaccatt ctgtttattt gcttacctat tagatatcaa taaaaaactg
    14581 cttaacagct atttcagctg ttaagcagtt ttaggatttt tagttcatat tcattgtttc
    14641 ttataataca gtgttcaata cttctcttaa agcacttgtt gatttgctga acattgcttt
    14701 ttcttcttcg ttgattgata attcaacaac ttcacgaaca ccattttcgt caacgattga
    14761 tggcacacct gtaaagatat ctttttcgtc gtattcacca gttaaataag cagatacagg
    14821 caatacagct tgttcgttat tcaagatagc ttttacgata cgtgcagtac tcataccaat
    14881 accatagtaa gttgcttttt tacgatcaat gatctcataa gcagcattac ggactttatc
    14941 agcaatcaca tctaattcat cttgggcaat acgatggtct ttttctacaa tttcaaacac
    15001 tggtttacca ccgacagttg tatgtgacca tgcagcaact tctgaatcac catgttcacc
    15061 taagatatag ccgtggacgc tgcgtgggtc aacttttaat ttcaatgcga tttctttacg
    15121 gaaacgagtt gtatccaatg ttgttccagt accgataaca cgtgatgttg gcagaccaga
    15181 ttcgttccaa gcgatatatg tcaaaatatc tactgggttt gaagcaacaa cgataatacc
    15241 gtcgaatcct gatcccatga tttcttttac gatttggcgc atgattgacg catttgtttt
    15301 aactaaatct aaacgagatt gaccaggttt ttggttgata ccagctgtga taacaacgat
    15361 atcagcatct ttacattctt catagccacc tgaccataca gccacatttt catcgcccca
    15421 agccatacca tctaaaaggt ccaatgcttc cccttctgct ttttcttgat tcacatcgat
    15481 caatactaat tcattagaga ttccttggtt gatcattgca taagcgatac ttgttccaac
    15541 aaaacctgta ccgacgatta ctactttacg acttgttttt ttcatgaata aacagctcct
    15601 tttcataact atttgttact ttcatgaaaa gtatagtctt gtttattcat tttatcaaca
    15661 tgttaattat aatagttgtt attataattt ttgatggaaa cgaattcct
//
LOCUS       DACEXQ010000036        15586 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.036, whole genome shotgun sequence.
ACCESSION   DACEXQ010000036 DACEXQ010000000
VERSION     DACEXQ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.036"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..1255
                     /locus_tag="IXH04_000905"
     CDS             <1..1255
                     /locus_tag="IXH04_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ1851534.1"
                     /translation="NSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTSISRM
                     ENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLVNMSD
                     AQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNTGMHP
                     LQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDYKSSG
                     RSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTETDDVE
                     KNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKDKFFT
                     EEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDVMLKE
                     NNYHRLEKIDKEEALRRILKRSEEE"
     gene            1252..4974
                     /gene="addA"
                     /locus_tag="IXH04_000906"
     CDS             1252..4974
                     /gene="addA"
                     /locus_tag="IXH04_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAQ1851535.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            5043..5384
                     /locus_tag="IXH04_000907"
     CDS             5043..5384
                     /locus_tag="IXH04_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1851536.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            5693..6736
                     /gene="pheS"
                     /locus_tag="IXH04_000908"
     CDS             5693..6736
                     /gene="pheS"
                     /locus_tag="IXH04_000908"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAQ1851537.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            6741..9161
                     /locus_tag="IXH04_000909"
     CDS             6741..9161
                     /locus_tag="IXH04_000909"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAQ1851538.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKEINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(9331..10341)
                     /gene="trpS"
                     /locus_tag="IXH04_000910"
     CDS             complement(9331..10341)
                     /gene="trpS"
                     /locus_tag="IXH04_000910"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAQ1851539.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            10595..11332
                     /locus_tag="IXH04_000911"
     CDS             10595..11332
                     /locus_tag="IXH04_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ1851540.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            11346..12161
                     /locus_tag="IXH04_000912"
     CDS             11346..12161
                     /locus_tag="IXH04_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ1851541.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            12174..12821
                     /locus_tag="IXH04_000913"
     CDS             12174..12821
                     /locus_tag="IXH04_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ1851542.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            12837..13493
                     /locus_tag="IXH04_000914"
     CDS             12837..13493
                     /locus_tag="IXH04_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ1851543.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            13655..14476
                     /gene="racE"
                     /locus_tag="IXH04_000915"
     CDS             13655..14476
                     /gene="racE"
                     /locus_tag="IXH04_000915"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAQ1851544.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            14479..>15586
                     /gene="rph"
                     /locus_tag="IXH04_000916"
     CDS             14479..>15586
                     /gene="rph"
                     /locus_tag="IXH04_000916"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAQ1851545.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQF"
BASE COUNT         5226 a         2682 c         3347 g         4331 t
ORIGIN      
        1 gaattcgtca tggcgaaatt ttgctttaca gatttttgaa agtcaggaac atttaaatgt
       61 tcctgtttcg atgccagaaa aaatggcgga agagctttat ggaaaagaga tttatacttc
      121 aatttcacgt atggagaatt tttataattg tgaatacaaa tacttcatgc aatttggttt
      181 gaaattgaaa gaacgaactg tttatggtct aactccagca gcaactggtg atttttatca
      241 tgaatcattg gatcgtttct tcaagctact gttttcaaac caattatcct tagtgaatat
      301 gtccgatgcg cagcgtagag actttactga aaaagtgctg caggaagtat tcggagaact
      361 tcggtttgag atattggata gttctgcaag aatgaactat atccgctacc agttaggaca
      421 gacgattcaa aaagtgacat gggctttgca aaaacagagc aaaaatacag gcatgcatcc
      481 tcttcagaca gagattctat ttggtcagat tgctggagca aaaggtatca caggtctgga
      541 attaccgttg agtaatggtg gaaaactcca tgttcgaggg aaaatcgatc gaatcgatgt
      601 agcttctgaa caagaagata cttggctaag cgtcgttgat tacaaatcta gcggacgttc
      661 attcgatgtc acagaagctt attatggcat ggctatgcaa ttattgacgt atctggacgt
      721 agcattgatg gatgcggtac gtttgactgg aaaggcggct gttaaaccag caggatcata
      781 ttatttacat gttcataatc ctgtattgac ggagacagat gatgtagaga agaatacatt
      841 gaaaaaattt agttatgatg gcgtttttgt cgatgatccc gctcttttag aagtactaga
      901 tcattcactg gaagcaaaag aacaatcact gatctatcca gtgaaaaaaa atgcgaaaga
      961 tgaataccaa aaaggtagtt tcagtaaaga caaatttttc acggaagaag aactgcaact
     1021 attcatggcg cataatcggg aaaatatgcg gttagctgga gatgcgatta cttccggaga
     1081 aatcaaacta aatccagctt ataaagataa acagcggatt gcctgcgaat tttgtccatt
     1141 cagaagcatc tgtacgtttg atgtgatgct gaaagaaaat aattaccacc gtctggagaa
     1201 aatagataag gaagaagcac tgcgtagaat attgaaaagg agtgaagaag agtgaatcaa
     1261 attcctttaa aaccgcaaaa tgaacgattc acagatgatc agtggcaagc gatttttgat
     1321 caaggagata atctattagt ctctgcgtct gccggatcag gtaaaactac agtgcttgtt
     1381 cgaagagtga ttgaaaaact aaaaatgggt tttgatatcg atgaattatt gattgtgaca
     1441 tttactgaag ctgccgcaag agaaatgaaa gaaagaattc aagaggcatt acaagaatcc
     1501 gtcaatagcg aaagtgaccc tgtacgtaga cagcatttta ctaagcagtt agttttattg
     1561 cccacagcga atatctcaac cttacatgct ttttgcctga cagttattcg acgttattat
     1621 tacttaattg atattgatcc agtctttcgc atgttaacag atgaaacaga aacgatcttg
     1681 atgaaggagg atgtgtggga cgagctgaga gaagcacttt atgcagaaaa tgatgaacga
     1741 tttttccaat taacaatgaa tttttctaat gatcgttcag atgacggctt gacgaatctg
     1801 gtgttttctc tttatgagtt tgcccgtgcc aaccctgacc cccaaaaatg gctagaacag
     1861 ttaagcgata attatcgttt gcctgagggg ctagcgaaat ctcgattata tcaaaaacaa
     1921 atccgtcctt tagttttggc agatatatac caatgtgtac agttatatga acagatgact
     1981 cagttagcac aaggtgaagg attagaaaaa atgcatgagc aagtagctgg agaacaacag
     2041 cagatcaaga atatctatga agcgttctcg caagatcggt tagaagaagc ttatgctggt
     2101 ttggaacaac taactttttc aacttttaag agcagtcgga aagcagaatt gaaagaaatt
     2161 tccaatgaag tcaagggcat gagggataaa gcaaaaaaac tgattcagca gataagtaaa
     2221 agttattttc ctgtttcccc aagccaaatg gaagaattaa cggacaaagc actaccgtta
     2281 gtcgaagaaa tgacaaaagt cacgcaaagc ttcatggacg gtttcagtat gcgtaaacgg
     2341 gaaaaaggtg tgctggattt caatgatctg gaacatttag cgttgcagat tctcacagaa
     2401 aaaacgaaag atgtatggct gccaagtgaa gcttcaaaac attatcgaaa aaaattcaaa
     2461 gaagtaatgg tagatgaata tcaagacgtg aaccaattac aagaagccat tttatactgg
     2521 ctaagagaac ccgatgatac aaaaggaaat atgttcatgg ttggggatgt caaacaatcc
     2581 atctattctt tccgtttagc agatcctagt ttatttatcg ggaaatacga gaatttctca
     2641 aagaaagaag gcggacgtcg aatcgttttg gctgagaact tccgctctag aaaagaagtc
     2701 ctttctttta cgaatcttat ttttgaacaa ctaatggatc cagcagtggg tcaaatcaat
     2761 tatgacgaag cagccaaact gatccaagga ttttcagact ttccagagaa tgaacagttt
     2821 gagccggaaa tcatgatcta tgaaaaagaa caggaagaat cagagattga gattccgacg
     2881 gatgatatac tggaagacaa aacagaaggc gaacttttta tgacaggact aaaaattcgt
     2941 caattaattg attcctcctt tatgatttat gataaaaaat caaaaaaaag tcgtccgata
     3001 gagtacaaag acattgttct cttgacacct acaaagaaga acaacttaac gattttagag
     3061 attttcaaaa cattggatat tccattagag atgaatgatg ctcaaaacta ttttcaagcg
     3121 acagaaattc gcacgatgat ctcgctgcta caattaatcg ataatcccta tcaagatatt
     3181 ccgcttgcgg ctgtattgcg ctcaccgatc gtgggtctga ttgaaccaga attagccagc
     3241 attcgattag ctaaccgtgc acatacttac tatgatgctg tgcttgctta tcaagcatca
     3301 aacgaagatg aattagcagc taagctggaa cattttggca agcaactaga gcattggcga
     3361 gaacttgctc gccgttcttc gatcactgac ttgttatggg atatctatta tgagacggga
     3421 tatctagagt atgtggtcgg tctgccagca ggggcacaga gacaagccaa tctttatgca
     3481 ttggttgatc gtgccaaagc ttacgagcaa agttctttca gaggattata ccagttcgtg
     3541 cgtttcattg aaaaaatgca agaaaaagat aaagacttag cagaaccagt catttcaata
     3601 gaagataatg ctgttcgcgt tatgacgatc catgcaagta aaggcttgga attcccagtt
     3661 gtatttttgt tagatatgac aaaggagttc aacttacaag acttgcgcaa tcggtatgct
     3721 tttgaagaaa aacttggagc aggaattcgc tatatggatc ctgaaacacg agtgttatat
     3781 gatacgttac cgtttcaagc catcaaattg gcaaagcaaa acaaattgtt gtcggaagaa
     3841 atgcgtaaat tgtatgtagg attgactcgg gcagaacaaa agcttttcat tgtcggttcc
     3901 tataaaaata aagagcaaat gatccaaacg tggtctgaag cagctgatca tgaggagtta
     3961 gtttttgatc ctgcgctacg tttgaagggc agaagcagct tgatgaattg gattggttac
     4021 ggattgatcc gtcatcctga aatgcagaaa tacttagagg aagaaatttc gacttctctt
     4081 cttcaacatt caaatgcaca gttttctatt tcatggatga atcagcaatc gatcattgaa
     4141 caaagacagt tgcttgctga aaaagaactg gttaacttag atcagcagat gaaagaagat
     4201 gaaactttat tagcagattc gttacaaaaa cgtctggcat atgaatatcc ttatcaagct
     4261 tcttcacaaa ccacaagtta tcaatctgta tctgaaatca aacgattatt cgaagatcca
     4321 gatgatacac aagaatcacg tttgactctg gaaagcagtc agaacaaagc agcatcccgt
     4381 caatttcgtt atacgcaaga acaactagca gaaccaaaat ttttgcaaaa agatcggcaa
     4441 gtgtcagcag ctgctgttgg aactgctacg catgctttgt tacaattact gccattagag
     4501 atgccaacta cagagagtat ccatcaaaaa ttacaggaat tggtaaggaa acggttagta
     4561 gacgaaaaag tcgcgaaaaa agtagatgtt tcttctatta tatggttctt tcaaacagaa
     4621 cttggtcaac agctgattgc caacaaagaa aacgtaaaac gagaacaacc tttttccatg
     4681 ctgcttcctg ctgacgaagt atttcaagac tatccaaatc aagaagatga actattaatc
     4741 cacggaatcg tcgatggata cctagaagaa aaagatcatc tcaatatcta tgattttaaa
     4801 acagatttta ttttacatcc ggatgatcca gcagaaatag atgcaatcgt ccaaaaatat
     4861 caaggacagc taagacttta ccaacaagca atgtcagaag ctttgaataa gccagtagaa
     4921 aatgttttcc taattttgct tcgagtgaaa caaattataa acattaacaa ataattataa
     4981 ttgagcaaac ttgacttatg ttttgtaagg attatgctat actctgggaa gaggtgagta
     5041 tgatggagct aacacagaca gaattcgttt tatgccctaa atttgaaaaa acattctcta
     5101 ttttaggaaa aaaatggaac ggattgatta tcgatgtact actcgaaaac ggtccgcaac
     5161 gctttgttga gcttgcggca aaaatcccga tggtcagtga tcgagtatta gtcgaacgct
     5221 tgaaggaatt agaaaaagaa ggaatagtca ctcgaaactt tgcctgtagc gaaaagagcc
     5281 gttcagaata tctcttgact gaaaaaggag cagacttgca aaaagcgatg cgtcaaatac
     5341 aagtttggag cgaaaaatgg atcacagagg aagaatgcgg ttgattttgt aaaaaaaatc
     5401 ttgtaaacta tagtccatac gaataagtaa agaacgatga cgaaaaagag tagtttggct
     5461 aaaccagttc agggagatct gtcgcagact gaaagcagat cctgtgtttg aaaaatgaag
     5521 ttcactttcc gagttcgtct gataaaatca gaccggcaat tgtcgttaac gaagtaagag
     5581 tgcggtgtaa gtcatttatc cgcaaattag gatggtaacg cgaagacctc gttccttaac
     5641 aggaatgagg tctttttgtt ttgtatttgg aatttaaagg aggaacaagc acatgtctct
     5701 acaagcacaa ttagagagct tgaaagaagc aacgctcgtc aaaatcacag cggcacaaga
     5761 tttaaatgaa ttaaatcaaa tacgtgttga aacgttagga aaaaaaggac cgatcacaga
     5821 agtattacgt gggatgaaag atctaagtcc agaagagcga ccaaaagtcg gaagtttcgc
     5881 aaatgagatc cgagatcttt tgacagaaga gatcgagcga aaaaaacaag tattagaaga
     5941 agaagcgtta aatgcagctt tggcaaaaga aacaatagac gtgacactac ctggtagaaa
     6001 agtaaaacat ggtacacgtc atatcgtgac acaaatcatg gaagaaattg aagatatttt
     6061 tgtgggtatg ggctatcaga tcgtagaagg atatgaagta gaatctgatc attacaattt
     6121 cgaacggatg aatctgccaa aagatcatcc agcaagggac atgcaagata ctttctatat
     6181 ttcagacgag atattgattc ggacacatac ttcaccagtc caagcacgga caatggaaaa
     6241 acatgatttc tctaagggtg ctctacgaat gatctcgcca gggaaagtct tccgcagaga
     6301 tacagatgat gcgacccaca gtcatcagtt ccatcaaatt gaagggctag ttgttgataa
     6361 aaacatcaca atgggcgacc ttaaaggaac attagaagtc gtaatgaaaa aaatgtttgg
     6421 ggaagatcgt gaaattcgtt tgcgtccaag ttatttccca tttacggaac catcagtaga
     6481 agtagatgtc agctgtttca aatgtggtgg tgccggttgt aacgtatgta aatacactgg
     6541 atggatcgaa attttaggag ctggcatggt acacccgaat gtgttgaaga tgtcaggaat
     6601 cgatccagaa gaatattcag gctttgcttt cggccttgga ccagaccgcg tagctatgtt
     6661 gcggtatgga gtgaatgata tccgtaattt ctatcaaaat gacttacgtt tcttaagtca
     6721 gttcaagggg gagtaagaaa atgttagttt catataaatg gctcaatcgt tatgtagatt
     6781 tatcgaatgt aacaccaaaa gaattagcgg ataagatgtc tgtgacagga atcgaagtag
     6841 agggagtaac ggttccagaa gaaggcttga aaaaaatcgt tgtcggtgaa gtcaaggaat
     6901 gcgtaccaca tccagattct gatcaccttt ctatttgtca agtcgatatt ggcgaagacg
     6961 aactgtctca aatcgtttgt ggagcgccta atgtgaaggt tggaatcaaa gtgattgttg
     7021 cacttcctaa ctcacgtatc gctggtaatg taaaaatcaa aaaaggaaaa atgcgtggcc
     7081 aagtatcaaa tggcatgatt tgctcattac aagaaattgg ctattcagat agtgttatac
     7141 cgaaagaata cgctgaaggt atctattata tgccacaaga agctgtaaat ggcgatccag
     7201 tttttcctta tttggatatg gatgattcga ttattgaatt atctatcaca ccgaatcgtg
     7261 cggatgcatt gtcaatgcgt ggcgtagctt atgaagttgg ggcaatttat cgtcagactc
     7321 ctacattcga tgacgaaaag ctggtagaat cctcacgtcc tgctagcgat aaaattcaag
     7381 tggaagttga agataaagaa gcagttcctg cttatcaaat cagaatcatt gaaggagtaa
     7441 gaatccagcc aagtcctcaa tggctgcaaa atcttttgat gaacgaaggc attcgtccaa
     7501 tcaacaatgt agtagatgtg acgaactata ttttgctttt attcggacaa ccactacatg
     7561 catttgacta cgacaaattg aaatcaaaag agatctttgt ccgccgtgca aaagaaaatg
     7621 aaacattagt aacactagat ggagaatctc gggaattgac aactgacaat ctcgtcatta
     7681 ctaacggcaa agaaccagtt gctttagctg gtgtcatggg agggttagac tcagagatca
     7741 cgaatgaaac aacaacagtt gcactagaat ctgctttatt tgatccaatt tcgattcgtc
     7801 gtacagcaaa acaatttaat ttgcgtagtg aatcctctgc acgttttgaa aaagaaatca
     7861 accatgcgac tgttggagaa gccagtgagg ttgctgcagc aatgatcgtt tctcttgcgg
     7921 gtggggaagt tctacaaggc gcggtcaaag gctcagagat caaagcagaa gacgtatcag
     7981 tgaagatttc acttgaacgc atcaatcaat atttaggaac atctcttgtc gttaaagaag
     8041 taaatgagat ttttgaagca cttggattta gctatgaaga aaaggagtat gtttattccg
     8101 tagtagtacc accaagacgc tgggatatcc aaatcgaagc agatattatc gaagaagttg
     8161 ctcgtattta tggatacgac cagttgccat ctacacttcc aagtggagaa acggttgctg
     8221 gaagcttgtc aaaagaacaa gaaactactc gtaaaattcg tacaatcttt gaaggaagcg
     8281 gattgagcga agcaatcagt tatgcattga caacagaaga aaaatcaaga cagtttgtta
     8341 taaatgaaag taatattaca cgtttggact ggccaatgag cgaagaacgt tctgtattac
     8401 gaatgaattt gatcagcgga ctattagatg atatcagtta taacactgcc cgaaaaaata
     8461 cagagattgc tttatatgaa attggtcggg tgttcttcca agaaaatgat ccacgtacac
     8521 atctgccgca agaagtaaaa catgcagcaa tcgctatttc tggtatttgg gaagaaaaag
     8581 attggcagac gaaagctcaa aatgtagact ttttcaccat caagggtctt ttagaaaact
     8641 tatttgaaca actaggcatc actgaagatg tccattatca aacagtgaca gaaatgaagg
     8701 aaatgcatcc tggaagaact gctgcgattt accttggcga aaaatttatt ggttttgtcg
     8761 gtcaagtgca tccaacgatt acaaaaagtt atgatattcc agaaacatat gttgctgagt
     8821 ttgatttatc tgctatagtg gaagctgctc aaaaagggat cgttttcgaa gcagtgacaa
     8881 aattcccagc agtcagccgg gacatcgctt tgcttgtaaa agaaactgtg acaaatcagg
     8941 aattaacaga agttatccgt tctgctgccg gaagattttt aacagatatc caattatttg
     9001 atgtctatca aggagaaaac attgaaaaag gacataaatc catggcgtat agcctgacat
     9061 tcgtcaaccc agaagcaaca ttgacagatg aagaaatcaa taaagcaatg gaaaaagtcg
     9121 aaaaagcgtt ggtagaaaac ctagaggcaa gtattcgcta agaaggaaaa tatagtaagt
     9181 aacgatcaag tagcttttga agaccataca tttgaaaaaa agatgtgaca caacttcgtc
     9241 acatctcttt ttttgatttt aaaaaaggaa tagcacagag acgagataaa aaaacaggcc
     9301 tttacagcct gcttttttag agtaaatgaa ttaatggaag taattgattc ccattgctga
     9361 ttttacttca gaaagtgttt gtgcagctac tttagcagct tcttcactac ctttacgtaa
     9421 gatttccata acagctgaag gatctttttc caattcttct cggcgcgcac ggatcggtgc
     9481 aaattcagct tccaatacat caatgaggta tcgtttgatc tttacatcac caagtccgcc
     9541 gcggcggtaa tgagctttca tttcttcaat tgcttcttta tctgttccga atacatccaa
     9601 gtaggtgaag accatattcc cttctacttg tccaggatct tgtacatgga tgtggtttgg
     9661 atcagtatac atactcatga cttttttctg taagacatcg gcaggatcgg agatataaat
     9721 accgtttcct aacgatttac tcattttgcc atttccatcg attccaggta atctacccat
     9781 tccttttggt gggaatacgc cttttggttc caccaagact tcaccatatg tatgattgaa
     9841 gctttgtacg atttcctgtg tctgctctag cattggtttt tgatcctcac caacaggtac
     9901 aagattcgct ttgaatgcag tgatatctgc tgcttgagaa acaggataga taaagaatcc
     9961 tgtaggcacg ctttcaccga attttttttg ttcgatttct gttttgacag tcggattgcg
    10021 ccgaacgcgt cctacgctga ctaagttcaa ataatacatg gttaattcag ctaattccgg
    10081 aatttgtgat tggataaata aagtagattt tgcaggatct aatcccacgg ctaaatagtc
    10141 taaagcaact tgcaagacat ttgatgaaac cttctcagga tttttagcat tatctgtcag
    10201 tgcttgcatg tcagcaatca tgacaaacag ttggttgtct tcatctgcct gcatagccac
    10261 tcgggttttt agagaaccga cgtaatgccc taaatgcagc ttcccagttg gtcgatctcc
    10321 cgttaaaata atatttttca aaaaagacac ctctttacga tttgtaagtc tgagcactta
    10381 atatcttacc gtttttcctg aaaagaaaca acagttccta aggaaaaaac aaggaaagag
    10441 gataaaataa ctaatagatt agaaatggaa gaaatattta tgggagaata aacgttctaa
    10501 gaaactttac aaattactaa actatcagat ataatgtatt ttattataca tctttaaaag
    10561 aaatcttact aaataataat gggagtgtaa ttctatggca atgattaaat ttgatcacgt
    10621 ggataaatat tatggcaagt tccacgcatt aaaaaatatc aatctcgaat tcgagaaagg
    10681 agaagttgtc gttgtcattg gtccttctgg ttcaggaaag agcacgatgc ttcgctgtat
    10741 caatggatta gagacgatct cttcaggtaa gctgctgatt aatgatactg acttgcatga
    10801 taaaaagaca aaactgacag aagtccgaaa aaacatcggc atggttttcc aacatttcaa
    10861 tttgtaccca aataaaactg ttctggaaaa catcaaatta gcaccaatca aagtgttgaa
    10921 gcaagaccag gcaactgcag tcaaaaacgc ggagaaattt ttgaaaactg tcaatatgtt
    10981 agataagaaa gattcttatc cttccatgtt atccggggga caacagcaac gggtggcaat
    11041 cgccagaggt ttagcgatga atcctgagat gctgctgttt gatgaaccta cttctgcttt
    11101 ggatccggaa atgattggtg atgtattaga cgttatgaaa aaattggctc atgacggtat
    11161 gtcaatgatt gtcgtgacac atgaaatggg ctttgccaaa gaagtggcgg atcgtgttat
    11221 cttcatggct gatggagaag tattggaaga cagcagaaat gtgagagact ttttcgaaaa
    11281 tccaaatgaa gtccgagcac agcaatttat cagtaaagtt atcaatcatt gataggagga
    11341 aaaagatgcg caggttaaaa aaaatacgcc tgattttcct tttaggcatt gcttttcttg
    11401 cactagccgg atgtaaagga aatgggttgg caaatgaaga tattttagag cgaagcaaat
    11461 ctaccaatga aatcatctgg ggtgtcaagt atgatactcg attatttgga atgatggata
    11521 ttgagagcag aactgtacaa ggatttgatg tagatattgc aaaagcaatc actaaaaaga
    11581 tccttggtga caatggaaaa acagaatttg ttgaagtcac ttctaaaact cggattccat
    11641 tattgaaaaa cggaaatatc gatgcaatta ttgcaacgat gacgatcaca gatgaacgaa
    11701 aaaaacaggt tgacttttca gatgtctatt tcgatgctgg ccaagctttg ttggtaaaga
    11761 aaggaagcca aatcaaaagt gtagatgatt tgaatgcttc tacaacggtt ctagcagtca
    11821 aaggttctac atcggctgcg aatatccgac agcatgcacc agatgcaaaa attttagaat
    11881 tagaaaacta tgcagaagca tttactgctt tacaatctgg tcagggagac gcgatgacta
    11941 cggataatgc gattttgtta gggattgcag atgaaaatcc agagtatgag ttagttggtg
    12001 gaacgtttac taatgagcct tacgggattg cgatcaacaa agggcaagaa aacttcttga
    12061 aagctgttaa ccaagcgcta gaggaaatgc atgcagatgg cacatacgat aaaatctatc
    12121 aaaaatggtt tccaaatgaa actgaaggaa aagtagagta agggggagag gcaatgagcg
    12181 aattgataca aacttatgga ccaatctttg tcgacgggtt caagatcaca ttgttttcga
    12241 gtgtcttagc acttgtgttt agtttgatta tcggaacgtt aatggcaatc ttccaattat
    12301 caaagaataa agtagtgagc gccttagcaa aggcatatgt agaatttttt agaaacattc
    12361 cattattaat catcgtgatg tttttttatg ttgtcgtacc gatttattgg tttaatctag
    12421 atggattcca agcaggtacg attggattaa cgatctatac atctgctttt attgctgaaa
    12481 cagtacgttc agggattcaa accgtaccaa aaggtcaaac agaagcagga ctatcttctg
    12541 gttttaccta ttcagaaatc atgcgttata ttgttttacc gcaagcattc aaaatcgtta
    12601 tcccgccatt aggcaaccaa tttatcaacc tcgtaaaaaa ctcatctatt ttagcaatcg
    12661 tagctggttt agatttgatg tatcaaggag acttgatcgc tagtgaaaca ttcaatacct
    12721 tcgatacgta tatcatcgtt gggttatttt acttagttat cacgctaccg ttatcttact
    12781 taatggttta tcttgagaaa aaatgggcgg ttcgttcata gaaggagggg aaatacatgg
    12841 attttagcgg cgctttttca tggatgaata tccgtttttt attggaagga ttaaaggtta
    12901 ctgttgaagt ttcattgttt tcgatcattt ttagtttctt gatcggtggt ctgacaggtg
    12961 tgattcgttt tgcgaatatc cctttctttt ccaaaatcct tggtgtagtg attgatatta
    13021 tacggaatct tccattatta ttgatcattt tcttcactta ctttgcctta cctcaaatcg
    13081 gcattcagat gaacatcttc tggtccgctg tcgcagcatt aacgattttt gagtcagcta
    13141 tgttgtcgga aattttccga gctggactca atgctgttcc aaaaggacag atggaagcag
    13201 gattatcaac gggactgact tatgtagaat cgatgcgaac gatcgtgtta ccgcaagcat
    13261 ttaaatcaat gatcccggca attgtgagcc aattgatttc actgatcaaa gatacttcat
    13321 tagcagtaat catttcttta cctgaacttt ctcatcaggc acgtattatt tatggacaga
    13381 acaccaatta tgtgcttccg atgttcatca tcatgacact tatgtacttc gtggtatgtt
    13441 acgcgctttc tctgctttcc ggttacttgg aaaagagacg gtacagttat taatagatga
    13501 ttcttttagc ggttgtggca aactaaagtc acaaccgctt ttcctctttc cattcgaaaa
    13561 aaagagattt cgattgtttt aagcaagtaa gagcacttga aaattcacgc taaaaagtac
    13621 acaataggga ggatagaatt tattgaaaaa aaggatgata cgattgacag ataatcgccc
    13681 tatcggattt attgattcag gtgtcggcgg cttgactgta gtaaaagaag ccctgaaaca
    13741 attaccgaat gaaaatattt tatttgtagg agacacagca cgctgcccat atggccctag
    13801 acccgcggaa caggtaatcc agtatacttg ggaaatgacg gattatctgg tggagcaagg
    13861 aatcaagatg ctggtgatcg cctgcaatac cgcaactgcg gtggctttag aagaaatcaa
    13921 agctgctctt tctattccag tcatcggtgt gatccttccc ggtactagag cggcagtaaa
    13981 aaaaacacaa aataaacaag ttggcattat cggtacgatt ggtacggtaa aaagtcaagc
    14041 ttatgaaaaa gcactgaaag agaaagtacc agaattgact gtgacaagtc ttgcttgtcc
    14101 caaatttgtt tcagttgtcg aaagtaatga ataccattca tcggtggcga aaaaaattgt
    14161 ggcagaaaca ttagctcctt taaccactaa aaaaatcgat acattgattt tgggatgcac
    14221 ccattatcca ttattacgcc ccatcattca aaatgtaatg ggagaaaatg ttcaactgat
    14281 cgattctgga gcagaaacag taggtgaagt atctatgctg ttagattatt tcaatttgag
    14341 caattcaccg caaaatggtc ggacattatg ccagttttat acaactggct ctgccaaact
    14401 tttcgaggaa atagctgaag actggcttgg aatcggacac ttaaatgtag aacatatcga
    14461 attgggagga aaataaggat gcgtcatgac gggagaaaag cccaagagct tcgcaaactg
    14521 gagcttcaaa caaatgtttt caaatatcca gaaggatcgg tagtgatccg ttttggtgat
    14581 acaacagtta tttgttctgc aacaatagaa aattctgttc cccctttttt gagagaaaca
    14641 ggaacaggat gggtgacagc agaatatagt atgctgcctc gagcaacgag tacgagaaat
    14701 cgtagagaaa gcagcaaagg gaaactttct ggtagaacaa tggaaatcca gcgtcttatt
    14761 ggtcgttcct tgcgtgcagt agtagacttg gaaaaattag gagaacgtag tatcatcgta
    14821 gactgcgatg tgatccaagc ggatggcggt actagaacag caagtatcac tggggcattt
    14881 gtggcattga aattagccat tgaaaaactt ttaagagaaa aagaattgtc agaagatcca
    14941 ataaaagaac atcttgctgc agttagcgta ggaatcttac cagatgggac ttgcgtcaca
    15001 gatttggatt accaagaaga ttccgcagca cttgtagaca tgaatcttgt aatgacagaa
    15061 tctggaaaat tcgtggagat tcaaggaact ggagaagaag caacgtttga tggtgaacag
    15121 ttgaatgaaa tgctcttttt tggaaaaaac gcgattgaag atttgatcaa agagcaaaaa
    15181 catgctttgc taacagagtt tgctcaaaat gatgagcgga tagaagaaac aaaaacaatc
    15241 atcattgcga caagaaatcc aggaaaagca gaagagtttc gaaatatgtt caaagaagcg
    15301 ggatatcatg taaaaacatt acttgactat cctgaattgc cagatgtgga agaaactggc
    15361 agcacatttg aagaaaatgc ccgattaaaa gcagaaacga ttgcacaatt gcttgatcag
    15421 ccagtcttag cagatgattc aggtttgaaa gtggatgctt taggcggtat gccaggaatc
    15481 tattctgcac gttttgcagg ggaacaaaaa agtgatgcgg ggaataacgc caaattatta
    15541 tatgaattga cagatgtgcc ggatgaaaaa agaacagcac aattcc
//
LOCUS       DACEXQ010000037        15553 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.037, whole genome shotgun sequence.
ACCESSION   DACEXQ010000037 DACEXQ010000000
VERSION     DACEXQ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15553
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.037"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..1244
                     /locus_tag="IXH04_000917"
     CDS             <1..1244
                     /locus_tag="IXH04_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1851546.1"
                     /translation="KDLRERPAALLQPKAPKPGKRIFLEYITPLWSRLSFNQKISYRN
                     LFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSLSSVSAKQFGPIIDYQAIVTLDSSTED
                     SRQKAADMLQKQEKVNEILAIHTQTVELRKKGHASQSLTVIVPSKTADLDRFIHLTDL
                     EENPRSLPKTGIAITQKVAELFDLSTGDHISLYDEDQKEWKVEVKEIVKNYLGSFIYM
                     SPEYYASVTDQSMMDNAFLLRTDKMTTKEEEMLSEKLLATDAVTNTSFMSKQIETQEE
                     SMANLDSVVLIFVVLSGLLAFIVLYNLTNINISERVRELSTIKVLGFFDKEVTMYIVR
                     ENIIFTLLGIIGGFGVGYILTDFILQQASMENVIFPLVITWWAYVLSAGLTIVFTVIV
                     MIVTHFKLKHIDMIDALKSNE"
     gene            1695..3323
                     /gene="treC"
                     /locus_tag="IXH04_000918"
     CDS             1695..3323
                     /gene="treC"
                     /locus_tag="IXH04_000918"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAQ1851547.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(3383..4564)
                     /locus_tag="IXH04_000919"
     CDS             complement(3383..4564)
                     /locus_tag="IXH04_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1851548.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            4816..6339
                     /locus_tag="IXH04_000920"
     CDS             4816..6339
                     /locus_tag="IXH04_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAQ1851549.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(6408..7136)
                     /locus_tag="IXH04_000921"
     CDS             complement(6408..7136)
                     /locus_tag="IXH04_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1851550.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(7149..8429)
                     /locus_tag="IXH04_000922"
     CDS             complement(7149..8429)
                     /locus_tag="IXH04_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ1851551.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(8570..9172)
                     /locus_tag="IXH04_000923"
     CDS             complement(8570..9172)
                     /locus_tag="IXH04_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAQ1851552.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            9417..9623
                     /locus_tag="IXH04_000924"
     CDS             9417..9623
                     /locus_tag="IXH04_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851553.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            9935..10654
                     /locus_tag="IXH04_000925"
     CDS             9935..10654
                     /locus_tag="IXH04_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ1851554.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(10735..11418)
                     /locus_tag="IXH04_000926"
     CDS             complement(10735..11418)
                     /locus_tag="IXH04_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAQ1851555.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            11636..13822
                     /locus_tag="IXH04_000927"
     CDS             11636..13822
                     /locus_tag="IXH04_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ1851556.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            14168..14578
                     /locus_tag="IXH04_000928"
     CDS             14168..14578
                     /locus_tag="IXH04_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ1851557.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            14592..14948
                     /locus_tag="IXH04_000929"
     CDS             14592..14948
                     /locus_tag="IXH04_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAQ1851558.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT         5588 a         2618 c         2986 g         4361 t
ORIGIN      
        1 tcaaagattt aagagaacgg cctgctgcat tgcttcaacc gaaagcaccg aagccaggga
       61 aacgcatttt tcttgaatac atcactccgc tgtggtcacg ccttagcttt aatcaaaaaa
      121 tcagttaccg aaatctcttc cgatataagt caagaatgct tatggcgatt atcggaattg
      181 ctggatgtgc cgggctgatg gttgcaggcg tgggattgaa agattctcta agttctgttt
      241 cagccaaaca atttggaccg atcattgact atcaagcaat cgttacattg gattcatcca
      301 cggaagatag tcgtcaaaaa gcagcggata tgctgcagaa gcaagaaaaa gtaaatgaga
      361 tcctagctat tcatactcaa accgtagaat tgagaaaaaa aggacatgct tcgcaaagtc
      421 tgacagtgat cgtaccaagt aaaacagctg atttggatcg ttttatccat ttaactgatc
      481 ttgaagagaa cccacgctcc ttgcctaaaa caggaatcgc tattacgcaa aaagtagcag
      541 aactatttga cttatctaca ggagatcata tttcgcttta tgatgaagat caaaaagaat
      601 ggaaagtcga agtaaaagaa atcgtgaaaa actatctagg tagctttatt tatatgtccc
      661 ctgaatatta tgcttcagtt actgatcaat cgatgatgga taatgcattt ttgctgcgta
      721 cagataagat gacaacaaaa gaagaagaaa tgctttccga gaaactgctt gccacagacg
      781 ctgtgacaaa tacgtctttc atgtccaaac aaatcgaaac gcaagaagaa tcaatggcga
      841 acttagactc ggttgtctta atctttgtcg tattatcagg actgctggct tttatcgtct
      901 tgtataatct aactaatatc aatatttccg agcgtgtacg cgaattatct acaataaaag
      961 tccttggatt ttttgataaa gaagtcacga tgtacattgt tcgagaaaat atcatcttta
     1021 ctcttttagg aatcatcgga ggatttggtg taggatatat attgactgac ttcatcttgc
     1081 aacaagcgtc tatggagaat gtcatctttc cattagtgat cacatggtgg gcttatgtct
     1141 tatcagcagg attaactatc gtatttacgg ttattgtgat gattgttacc catttcaaat
     1201 taaagcatat cgatatgatt gatgcgttga aatcgaatga ataaaaacag aggttgtgaa
     1261 atcagcggtt tgacctgagt attagagaaa caaacggcaa aatagttttt catattttgc
     1321 cgttttttga gctaataccg aaggacagct gattgaacac catgaatcag aggttgtaaa
     1381 aaagccgtcc tgcatcgagc aaaaagaata ctcaaataaa attagctcct catgttttac
     1441 atttgagaag actgattgcc gtagataaaa gcttttgaac accatttata gaagaaacaa
     1501 gaatattttg ctattttaaa caagaggata gacaaataca tgtctgatct cttgtttttt
     1561 attgggcaac agacctctta gaatgtataa agataaattt tacaaacgct ttcggttcag
     1621 ttataataat aaatgataac ttgtatatat aagttgtggt aggtaataaa tcagttgaat
     1681 ggaggtaaat atccatgaca tttaaagaca aagtcattta ccaaatctat cccaaatctt
     1741 acaaagacag taatggtgat ggaattggag atttaaaagg aatcaaagaa aaactcccgt
     1801 atctgaatga tctaggcatc gacatgattt ggctaaaccc tatttatcca agccctcaaa
     1861 aagacaacgg ttatgatatc tctgattaca cagccatcga tcctatcttt ggtacgatgg
     1921 aggaattcga acagctaata gccgaagcaa agaaataccg gatagaaatc atgctcgata
     1981 tggtgctgaa tcacgtttcg acagaacatg aatggttcaa aaaagcacta gcaggggacc
     2041 agcattatca ggacttcttc attttaagag aagagccaac ggattgggta tcaaaatttg
     2101 gtggaagtgc ttggtcgcca tttggagata cggggaaata ttatttgcac ttatatgatc
     2161 ggacacaagc tgacttgaac tggcggaacc aagcagtaag agaggaacta ttcaaagttg
     2221 ttcgtttttg gatggaaaaa ggggtaaaag gattccgttt cgatgttatc aatgttatcg
     2281 gtaaagacga acaattgaaa aataataaag aaaatgatgg caaacctgaa tatacggaca
     2341 aaccaatcac ccatacgtat ctaaaagaat tgaaccaagc aacgtttggg acggattcca
     2401 ctatcatcac agtcggtgaa atgagctcta cgacgatcga aaattgtatc ttgtatacag
     2461 aacctagtag agaagaatta tcaatggtct ttaatttcca tcatttgaaa gtcgattatg
     2521 aaaatgggaa taaatggtct actcctccat ttgattttgc agaattaaag cgactatttc
     2581 atacatgggg agaaaagatg agtgagggta acggctggaa tgctctattt tttaataatc
     2641 atgatcagcc acgtgcgctc aatcgatttg tagctatcga tcagtatcga aaacaaggag
     2701 cagagatgct agcaaccatg atccatttga accgtgggac gccctatatt tatatgggag
     2761 aagaaatcgg catgatcgac ccagactatt catcgatttc cgactatatt gacgtagaaa
     2821 gtttgaatgc ttataagtta ctccttgaac aaggattcac agaaaaagaa gcgtttcgtc
     2881 ggatcaaagc aaaatctcgc gataactctc gaacacccat gcaatggtca gacacagaac
     2941 aagcaggatt tactacaggg aaaccttggt taaaagttgg aggcaagcta gaagagatta
     3001 atgtagaaaa agaactatct tctgaagatt ctattttgtc ctattacaag aaattgattc
     3061 ggctgagaaa aacatatcca atcgttgcac aaggagatta tcatgcttat gaagcaaatc
     3121 atccggaagt ttatggcttt cttcgccaat ttgaaggaca gcagctgctt gtattaacaa
     3181 atttctatga taaagataca aggatcgcca tcccagaagg attcacagaa gccaatattc
     3241 ttatcgataa ttattccgat actgttcttc atcaaagtct ggagctaaaa ccataccagg
     3301 caattgcttt gctgaaaaat taaaacaaaa acacaaaacg tcgttcctac gagctattct
     3361 cgtaagatcg acgttttgtg ttttatttaa aataatcttc ttcagaaaat gaagagtttt
     3421 catactctcc tttatcgcca taaaatttat tgtagcgttg tttgaattta cggaaaataa
     3481 aagtaacgag taatttgact aacgtataga taggaatacc aaaaataacg cccacaattc
     3541 ccattagatc acccatgaca agtaaaacga ttaagatcgt aatcggatgg atctgtaaac
     3601 gatcacccat tactcgagga atcaccaagt caccgtggag aagttgtacg gcaaaccaga
     3661 caatgacaaa tttgacagcc ataaaagtag agtcttggaa tgcaatgatc aaacctggaa
     3721 agaaggcgat aaacggccca atatatggaa tgatacataa caacccagca gaaatagcta
     3781 aaacactagc aaattctaat ccgatcagta agaaacaaac ccagtagact gctccaagga
     3841 taaatgaagc aatgatttgc ccctttagaa atgaacccaa ttgttgatta gcaatctttg
     3901 tcaaattttt aaaatctgta cgaaatgctg gtgggacaat cgctaaaact gactgataaa
     3961 atttttgagg atctctcaaa aggaaaaaag cgacaattgg accagtaaat aaagtaataa
     4021 aaatagctgt aacagttgaa aaaatgttgc tgatccgttg tgcgccactt tcccaataat
     4081 ttccaataaa tccagctata tcagacgtca cgtcgtcaat agaagcgaaa aattgattaa
     4141 atgaatcggc aaaaggtgtt ttctccaaaa attgtcggag actctcttga aaatcttgaa
     4201 acaaatctgg caaactactg atcaatgtct ttgcctgttc ttcaagaaaa ggaataagcc
     4261 atatgcttcc aagccccaat aaaaataaaa taatcacata aacgataatg actgacaagt
     4321 tcctcgaaag gtgcttgctc aaacgtttga tgactggttc caaaagataa tacaagatca
     4381 gtgcaaacaa taatgggggc aagatagtag cgataatgat tccgacaggc ttgaatataa
     4441 acgagatctg atggaaaaaa taaatgatca gcgcaagtaa gataactatt cctaaaacat
     4501 agtaagaggt ttttcctcct aaaaagcgga tcaaacgatt cgttgtatct gtttcttttt
     4561 tcactctttt tcctccttta tctataaatt cattatagac aaaagcgggt ataaaaggaa
     4621 aatgatagag gttgattgca aaaatatcgt caatcattca tataaacttt ctcatcaatt
     4681 aaaataagaa aacgcttgac agttctttta accatatgat aaattaaatc ctgttgctaa
     4741 aacgtaacta ttacggtttg cgaaaagcga atcggaacat tcaaatgaaa gaagatgaga
     4801 agaggagtgt cataaatgaa acgattgata ggtatattgg ccatgctggt tttagcaggt
     4861 atgttaactg cttgcggggc aagtgggaaa gccgaggaca gtaaagaaaa attgagcgtg
     4921 atgacgacct tttatcccat gtatgatttt acaaaagcaa tcgtcgggga tgaaggagaa
     4981 gtagagttat tgattccagc agggactgat tcccacgatt atgaaccttc tgctaaagac
     5041 atggcaaaga tccaagatac agatatcttt gtttataatg atgaaaacat ggaaacatgg
     5101 gtccccgcaa tccaaaaaac attacaagaa ggcaatgtcc atacgataaa agcgacagaa
     5161 ggtatgcttt tacttccggg aagtgaagaa ggacatgatc atgaccatga acacggggaa
     5221 gaaggccata cacatgagct agacccacat gtatggctag ctccaagttt agcaatcaag
     5281 caggtagcaa acatacgtga tcagctgatt gaagcttacc cagaaaaaca ggaagtatgg
     5341 acaaaaaatg cagcagccta taccgagaaa cttcaagcac tccatcagct ttatcaagaa
     5401 acctttaagc aagcaaaaca aagatctttt gtcacgcaac atgctgcatt caactatcta
     5461 gcgttagaat atggattgaa tcaagtatcg attgctggtc tttcttctag tgaagaaccg
     5521 tctgcggcga gaattgctga actgaaacac tttgttaaag aacatggaat caactatatt
     5581 tattttgaag aaaacgccaa agatagtatt gccagaacgt tagcaaacga agcaggtgta
     5641 tctttagaag tattgaatcc tttggaagga ttgacgaatg aacaaataga aaatggagaa
     5701 aactatcttt ctatcatgga agcaaacctc gaagcgttga aaaaaacgac agagacagaa
     5761 aacccgctag aagatacttt gacccccaaa aaagaaaaga gcgtctacaa cggttatttt
     5821 gaagacgagg atgtacaaga tcgctctctt tcagattggg caggaacttg gcaaacagta
     5881 gatacgtatg tagaagatgg gacttttgat cctgtttttg aatataaaga aaaattgaat
     5941 caggataaga cagcacaaga atataaagaa tattatacga agggctatca aacagatatt
     6001 gcttcaatca agatcagtga gaaagagatg gcatttacgt ttaagaatgg aacaacgaaa
     6061 cgttcaaatt atcgttatgt agggaaagaa atcttgacct acgaggcagg aaaccgtggc
     6121 gtaaggtatc tattcgaagc agaagataag gaaagtggcg cttttagata tattcaattc
     6181 agtgatcaca cgattgcacc ggctgactcc gaacattttc atttatatgc aggaaatgaa
     6241 agtcaagaag tattgctgaa agaaatggag aattggccaa cattttatcc agatacaatg
     6301 gatggacgtg aaattgcaca ggaaatgctt ttgcactgat aggcaaaaaa gaaccgaagg
     6361 gtttggatcg gctggtcatt tagtcggtcc aaactattcg gttcttttta tgaaatcaat
     6421 ttctttaatc cacggataga ggctgggatg gtctcgccgt ttgccaacgt gacttctctg
     6481 gtttttttat taatcgaccg cacattttgt gtattgataa cataggattt atgacaacgg
     6541 ataaaattcc gatgcatttt ctcgatttct ttgatcttct catagaattg taattgggca
     6601 tgagtcaaat ggacttccag tttatgtggc gctcctagcg aagtagaaaa atattggatg
     6661 tctttgatcg gtaaaaattt gatctcatca ttaaactcta ttttgaagat atctttttgg
     6721 atttgggaca tttgaaaatc ttcgaaaatt ttgctcagga tatgctcgac attttctttt
     6781 aaatcataaa gatcttcttt gacgatataa tcagtcggtt cgatgttgct ttttaagatt
     6841 ttcatcgaca tttccgtatg ggaagtcaca tagattattt ttcctaaagg atctaattca
     6901 cggatcaatt taccgatcgc tacaccatcc atttcactgt cttctagttc gatatctaaa
     6961 aagtaaatac caaaagaaga agttctgatc ttggctgcat tgagaagttc ttccggatca
     7021 gaggtagaaa tatggacata actgtctaag ttttcaatca tgattctatt tttgatcacc
     7081 gttgataaaa cttctaattg ttttggatcg tcttcacaaa tataaatggg aatcatatca
     7141 ttactccttt attttatttg tatataaatc gtttgagaaa aacgctggtt ttcaattgat
     7201 gtttcaagga aaagataagg attggtcttt gtcaattcat ccaagataaa caagccaagg
     7261 ccttcgtttt ttggatttgt ttttgttgag aacccttttt gtttcaactg gtttaacggt
     7321 aatgtatttg ttgttgtatt gatcacacga atcatcacgg aatgattctt cttgagtatg
     7381 gtcaattcga tttttttctt ctttgcagca ctagctcctt caatcgcatt gtccaagata
     7441 ataccaagca tacgtacaag gtttaccgta tgtttcgtat ccagttcgat tggctcagga
     7501 aaatgaagct ggacgatcaa ccctttttcc tgtgccatca tgagttttaa tgacagtaaa
     7561 ctacggatct caggaacttt catattatcc aacatactta gattggccgg aaaaaggttc
     7621 atcatctggc gtgtaggtaa tatcgtttga tcataataag accgtaatcc ttctaagtcg
     7681 ccttctgaaa tatagctgtg aagagaaaaa agcagatttt ggtaatcatg tttgaattct
     7741 ctcaagtctt gatagttttt ttctagatct ttgatataat cctgttgcat cctgacttgt
     7801 tgctctaata actgatcagc taatttctta ttagagattt tccaataata aaccaagatg
     7861 atcaggccaa cacacagata tagaatgaaa aaactcgtat tcaaagctaa gacatcaggt
     7921 gtttcaccag aaaaacgggt ataaaaaata atgatatagt atgtaaataa tatcaatgtc
     7981 ccgatcgtac cgatcatacg gcggtccaca gtgtgaaacc aagtctctaa cgattttttt
     8041 aaacaaaaag ctaataaagc agataaaaaa gcggataaga caatgtgaaa atactgcaat
     8101 gaatctcctg gttcaataac cgaagatttc aaaacaaaaa aatcgccgat gcttgttaag
     8161 tgatctccta aaagcgacgt caataaccca aaaggaagcg cgtaaaccaa ttgttgacgc
     8221 gaagtcgaaa agaagaaaaa agtaagcatc agcattccta tagcaattag agcagtaaaa
     8281 atccctaaaa tcgaatagac ccatccaatc aaaatacaag aaatcagaat gactcccgca
     8341 aacagatatt tgtatcggat gtgatggtca atcaataaga tacaaattgt gtaaatcaaa
     8401 tattgcaacg ctatggacca taaaaacata aagtacagct cccattgatt tttaccaaat
     8461 tatagcacaa ttggcgtatt atttttttaa tttttcaggg attttgaatt cagaactcat
     8521 accaaagcag cagccagata caaaaaagtc agataagtta gttaattgtt taatgattgt
     8581 ttttttcatg aaagattcct ccaaaattca tcaagtagtt tgtaatagga agcaacgtag
     8641 ctatttctaa aacaatccca agtagcaaat aggtagaaaa gtaagaagct gagaaaaata
     8701 acgtaatgag agaaaaaatt agaaacatgc tgatcgcctt ctttaataat gggggacgtt
     8761 tctcgggttc aatgggattg attgctgtac ctcttgatcc atagcgaagc agtaacaaaa
     8821 agcagctgat taacaaaaag accatcattg ggaaggagat agtcaattgc agatgtttca
     8881 tcaccaaggg aacgccccat aaataaagta gtgtccacag cagacaaacg aattcagagt
     8941 cggaatgcaa tccataagca tatctgcgga tgcagatata agaaagaaag gtgattcctg
     9001 tttccgccca tgagcctgtc accagagaaa agactagaac gaccgctagt tttccaagat
     9061 tattcaataa aacttctaag acaaatttta cttggacata caccatatct tcttccgatt
     9121 tgtctccgct gaataaggct tctaataatt tgttcacaac tttatctttc atctcatacg
     9181 tacctccacc acaaatttta tgctaaaaaa aataaaaaac gtcttttgtt tcataaaaag
     9241 tggttttgac atcaaaaccg tcatgatctc ctaaaaaaat cttcgttttc caagaacctt
     9301 accaaaaaaa gtgacagttt tgatacgaaa agtacttttt atgacaagtt tgtgcttttt
     9361 cgagtgattt atgttaaaaa attactgacc ttataaaagg tgatggaggg attcttatgg
     9421 aaaatgaaca attgtttgaa gtcggcgaaa catatgcatt taaatctgat ttatttgaat
     9481 caccaataaa aggaaaaata gaaaaaatct atgaaaatag tgtgatgata cgtgtcatga
     9541 aatgcgcagt agaagacaat gataccgctc ataatctgca ctggcagtta gttgtacgca
     9601 aatcaagtac aatagaagaa taaaaacaga taaaatgttg agagcataaa gaggttgtga
     9661 gtctcccgtt tagctctgag ttacaaggaa taatgttaaa aaacagcttt tcatgtttta
     9721 tgaatattgc cttgtgaccg aggtgttggc aggagaatac tgtttatttg aaaaaagggc
     9781 tgcgacaaga cttttgtcac agcctttatt ttcatgaata ggagttactt ttggctactt
     9841 ttagagaagt agcttttaag gagcagataa agtcaaggct tttatttcta taaatgaagt
     9901 agtaaaatga agaacagatt ttattacagg aaggatgccg ataattgtga tcattgttgc
     9961 aggaacgatc ggtgcaggga aaagcacact aactgaaatg cttgcccaag atttagaaac
    10021 aaaacctttt tatgagaacg tggaagataa tgaagtattg ccgctatttt attctaatcc
    10081 agaaaagtat acttttttat tacagatttt ctttttgaac aaacgctttt tggcaatcaa
    10141 agatgctttc agccatgatg acaacgtact agaccgctcc atctacgaag atagtatgct
    10201 gtttcatttg aatgcagatt tagggcgtgt ttctgaagta gaagtaaagc agtatgaagg
    10261 tcttttagaa acgatgctga aggagttaga agaaatttcg ccacaaaaga aaccagatct
    10321 actggtttat attcgtgtat cctttgaaac tatgttagcg agaatcaaaa aaagaggccg
    10381 ggaatatgaa cagctagaac aagatcctga actctattcc tactataaag aactcaatcg
    10441 acgatacgaa gagtggtatg aacagtttga tatatgtccc aaaatagtga tcgatgggga
    10501 caaatatgat ttcgtagccg atccggcatg tggagaacaa atcgtacaag aaatcaagat
    10561 gcgggcaaag aaaatgacag aagaggcaga tgcccagtcg atccgctaca ctacaaataa
    10621 cccgaaagca aagggaatct ttacaactaa ataatataaa aactaagcaa attgctcact
    10681 cgcagatatt tctctgtgag cagcatcttg cttagttttt ttgattcatt gagatcaaat
    10741 agtgaaccga ttcgtatgat aaaattgcca taaatgaaat gtctgagtga gggccatgat
    10801 caaaaataaa atggaaacga tcaaaatcga atgggcgcca aacagatgat gacagaaaac
    10861 aaggacaata gctcctgcga taaaggcaag aacgacactt agatagtgga aaaatcgatc
    10921 aaaatctgct ttttctttag aaatacaata atcatataag ttcgttgcta ccccttttaa
    10981 atttccagtt gtaaataaat tagtgtaggc acgcccgttg attttatcaa aagctaccca
    11041 ctggatcgat gcagtaaaag caatcaaaat cgttactaat gcgggatgct catggacggg
    11101 tgtcaaacta acaaagaaaa aaatcactgc ttcataatat agaacaaaca agcgccagaa
    11161 atgtttttct ttttttctaa aataatcaat tagatacttt gttagaagaa tccctaagaa
    11221 aaagaaaagg gtagacaata cttttttacc aacacttagc cattctccgt caaatgcttg
    11281 gatgatcgat agaatgatat tgccagtttg agcagaggca aaagcaccat attggatata
    11341 agtataactg tccatggctc ctccgataaa tgtcagcaaa agtcctattg tacgcccctc
    11401 gtgaaaggaa gtctgcattt ttttcatcac cttttatgaa tatttttgtc ttccttatct
    11461 tagcataaac gattgaaaaa aaggaaaaat tttcagaaaa ctaaaatcat atcgcttccc
    11521 attttcattt ttagaaaggt ctgttagtat aaagaaaaaa tagacaacga aactttaaat
    11581 aaccaacttg aaataaacaa attcaaagaa gaaagatggg aaggaaatca atcagttgaa
    11641 tgaaaaagaa attctccgat tacaaaaaga attgaaacgc tgggagaaca agtataaaaa
    11701 gcttcaacaa gtctttcggc tacaagaaga aatcattgct acctatgaag cggcttatgg
    11761 cgaagcaccc ggacttattg aggaagaagt acaagtaaaa gaaagtgaag gaaacagctc
    11821 tttctttaaa atgatggaga aacgttccta taatcgcttc aatcaagaac aaaaagcagc
    11881 tatcacatat gatatgaaga aacatcttcg aatcatagca ggagctggga gcggcaaaac
    11941 gcaaacaatc tgtgcaaaag ctgtttatct gatgacgcaa aaacaagtag acgaagaacg
    12001 catactaatg atcactttta caaggaacgc tgcaaatgaa ctaaaaaaac gagtagataa
    12061 tttcagccag agaaaaaccg ctgtccatat cggaacattc catagtatct tttttaggat
    12121 atacaatgaa atttgtcgaa aatttcctga agtagcaatg caaggaatac aaggagactt
    12181 ttcaaaggat actgcacaaa aagtgaatgc tgttttgcaa cagctgatac gtaaatataa
    12241 tctttatata tttgatcaat atggagaaaa aacgattgct tctcggttag actattggca
    12301 gaatatgaac ctttcagtag aagaaatggt tcagttagtt aaagaaaaat atgattccat
    12361 tgataagaat gcacgacagc cgatatcaga acgactgtat ggattgctca cggaactcca
    12421 agaacaaaaa agaagccagc aattgctaga gttcaatgat gtactccaaa atctgaaaag
    12481 cgctttggag aatgaggaga tccagcatta tatcggccaa aaatatgatt atctattcat
    12541 tgatgaattt caagatacta atccacttca atgggaaatc gtgaaacgga tgacaaagga
    12601 aaacaggatc aagttgattg ttgttggaga tgatgatcag agtatctatg gattcagagg
    12661 atcagagcca gcatacatca agaactttga aaaagaatat cctaccaaaa ctcttttctt
    12721 attaactaat tatcgttctc gggcagctat tgtccaagga gcaaatagat tgatcagcta
    12781 taataaaaat gatcggatac caaaggcgat gattccagca caaaaagaag caggtgttat
    12841 ggaagcctat ttgttttctg atactaaagc agaaagtcag tggctaatca aacaaattca
    12901 gacatttatt caaaaaaatg ggaaatataa ggaatcaatc atattatacc gatcagcttc
    12961 acaaacgcag cagcttgtcc aatctctgct gaaaacgaat attccttttg tactggaagc
    13021 agatagtcca tatgaaggta tctttggtat caagtctttc caacatttct atcaaaaaat
    13081 tgttcattgg caaacagcac tgaatctgca aaagaaaaat caggcctacc agcaattgct
    13141 ccggcagctg atggcagact gctacctgaa aaaaagtgaa tgcgatcaat acttttctcc
    13201 taaatatcaa aatatagccg ttgcagatta tattttgtcg ataagcccca atttaaaatc
    13261 gaaatctgca gaaatccgag agtttgagga agcgttgcgt aaaacagacc agcatcaaga
    13321 aatctattcg cttgttcaaa gctatcttca tctgccacgt atagctaagg aaatcgacca
    13381 gagtgatcaa gaatggatac gagaagaagt aaagcagcat gctactttca gaagactcca
    13441 agccttatcg caagaaaccc aagcgacagc taaagaatta aaaacccgct tgattgctta
    13501 cagacaagga aaacttgatg cactatgtat ccagagcatt cacaaaagta aaggtcttag
    13561 ttaccgaaat gtctttttga ttggttgtaa cgaaggaagt ttgccttata acggagcgac
    13621 agaaaagaaa gtaatcgact ttaatgaaat aaaagcagaa ccagtaacga cgatagaaga
    13681 agaacgaaga ctattttatg ttgcgatgac tagagcaaga aatcgtctgt atctatgtgt
    13741 acctcaaatg aaaaattcaa gaaaattgaa agtatcgaga tttatcaaag aaaccgggag
    13801 tcgtgtgaaa aaaggaaagt gatagaagag agggtgtgac agaagtggtc agtttcaaga
    13861 aagaagaagg aatgtacgaa aattgcttct caaatttttg tgaaatttca gcttattttc
    13921 gaagaagcta tttctggaac acagtttatt cggaaaaggg aggtgtgaca agaattttgt
    13981 cacacctccc ttatttattt tgaaaatgag aatactatta tcaaaataga gaatatatag
    14041 aaaaattctc attattcaaa cgtaaataat cagttatttt gatttttttt atattttatt
    14101 agctaataat tggtatttta aaagaaatta ctgtagaatt ggattataat taaaaaggag
    14161 tgagaaaatg ataaaagtat atacttcgcc tagctgtact tcctgtagaa aagcaagagc
    14221 atggttgcaa gcgaatcaac ttgaatttga agagaagaat atttttgccg aaccgttaac
    14281 agaaagtgag atcaaaaaga tcttggcagc gactgaagga ggagttcaag atattatttc
    14341 cactcggtcg aaaatttacg aaaagctggg catagatttt aacgaattaa ctttaaagca
    14401 aatgatcgtc ttattcaaag aatatccttc attattgaga cgacctattt tgattgatga
    14461 aaaaagaata ctaatcggat ttaatgaaga tgagatccgg tcatttgtcc ctcgtgaaat
    14521 tcgatgtgtt gccagaaatc tgatctattt acaaaacaat aacatcttaa attcataggg
    14581 agggagcctt cgtggaagtg aaagtctatg gatcagcgca ctgtgtaaca agtctgaagg
    14641 cgaaacaatg gttagaacac catcaattgc cttatcaatt tgttgactta gaagaacgcg
    14701 atctaagtac agaagaagca gaagaattat gcagttttga agaagtgcaa gcagaacagc
    14761 tatttgcgac ttggtctgag gcttttcaca acattgcgat tgacctttcc tgtgtaggaa
    14821 aaaaacaact gattttttta tgcacacaac ataccaatct cctcagacgt cctttgatta
    14881 ttatcgatga agttctattt attggataca atgagcagtt gatggaacag cttatcttaa
    14941 ataaatagac tttaactgtt ttaatcaagt cactgagcat ttcatacagt tgtaacagtc
    15001 gaacacttag catcaagcgt gttatgtgtt ctttttttga ctttattttt ctactataga
    15061 gacatgctca atttatgtgg tcaaaattca aatctaggtg gggatataga gttaaaaata
    15121 tattgacatg tgtaaattta catgatatat tcatttctgt tgttttaagt agcaaagcaa
    15181 taaaatagta ttttaaaaaa catataaaat agttgttgac atgttagaaa tgacatgata
    15241 agataactga gttgttaaaa aagaaccact tgtgaagctt gattctatcg aaaaaaactt
    15301 caaaaaagtt cttgacaaat aacaaatgat aagttatgat aaatgagttg ctgtaagatt
    15361 tcgaaaaacg aaatcaaaaa aacttttaaa aaagagttga caattacttg tctctctgat
    15421 atgatataaa agttgctacg gcagccaaaa acgaatcggg gaacactcct gatgaagtag
    15481 acctttgaaa actgaacaaa gtaagacaaa ccaaatgtgt agggcgtctt gattcaattc
    15541 aagacaacaa aca
//
LOCUS       DACEXQ010000038        15009 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.038, whole genome shotgun sequence.
ACCESSION   DACEXQ010000038 DACEXQ010000000
VERSION     DACEXQ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15009
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.038"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(273..1154)
                     /gene="galU"
                     /locus_tag="IXH04_000930"
     CDS             complement(273..1154)
                     /gene="galU"
                     /locus_tag="IXH04_000930"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAQ1851559.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(1197..2216)
                     /locus_tag="IXH04_000931"
     CDS             complement(1197..2216)
                     /locus_tag="IXH04_000931"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAQ1851560.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(2849..3853)
                     /locus_tag="IXH04_000932"
     CDS             complement(2849..3853)
                     /locus_tag="IXH04_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1851561.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(3866..4915)
                     /locus_tag="IXH04_000933"
     CDS             complement(3866..4915)
                     /locus_tag="IXH04_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1851562.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(4932..5753)
                     /locus_tag="IXH04_000934"
     CDS             complement(4932..5753)
                     /locus_tag="IXH04_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1851563.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(5740..6519)
                     /locus_tag="IXH04_000935"
     CDS             complement(5740..6519)
                     /locus_tag="IXH04_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1851564.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(6536..7006)
                     /locus_tag="IXH04_000936"
     CDS             complement(6536..7006)
                     /locus_tag="IXH04_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1851565.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(7018..7437)
                     /locus_tag="IXH04_000937"
     CDS             complement(7018..7437)
                     /locus_tag="IXH04_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAQ1851566.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(7518..10286)
                     /locus_tag="IXH04_000938"
     CDS             complement(7518..10286)
                     /locus_tag="IXH04_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1851567.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            10486..10710
                     /locus_tag="IXH04_000939"
     CDS             10486..10710
                     /locus_tag="IXH04_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851568.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            11489..13204
                     /locus_tag="IXH04_000940"
     CDS             11489..13204
                     /locus_tag="IXH04_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1851569.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALT
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            13207..14970
                     /locus_tag="IXH04_000941"
     CDS             13207..14970
                     /locus_tag="IXH04_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1851570.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
BASE COUNT         4362 a         3097 c         2585 g         4965 t
ORIGIN      
        1 aaaaaattcg cctttttatt ctactatgaa tcttctggct tggctttcat ttctcttatg
       61 taatcatttt cttttataca acttaaaaaa aaattaaacc attagcataa tattttctga
      121 atctattcac gaaagtcttt ttctatcttt cgttttccac gactcaactg atctagagtc
      181 aaaaaactaa aacgacagtt gcgcaaatga cgcacaagct gccgctttaa taacaaatag
      241 cctttcattc tcagcaggcc acttatttcc agttaacttt ctgttcttag ctttttactc
      301 aactggataa tatatgcact taatgaagtc ccaatttccg aatgttgaag tccatattca
      361 atatttgtct tcatatatcc taacttatcg cctacgtcgt atctttttcc tttaaactct
      421 ctcgcataga ccgcctctgt ctggttaaga gtctgaagtg catccgttag ctgtatctcg
      481 tttcctgctc ctggttcttg agtttctaat atctcaaata ttttaggagt cagtagataa
      541 cgtccgataa tggctaaatt acttggtgct tttcccattt caggtttttc tacaaaccct
      601 tttactctgc aacgaccctt ttcgctatct gttttaggat caataattcc gtatttagat
      661 gtttcatttt ctggtacacg catgacggca acagcggaag cttgtgtttc ttcataatcc
      721 atcatcaatt gttgagtcaa tgggatatca tctttcatca aatcatcccc cagcatgacg
      781 acaaacggtt catttcctat aaatgctttt gcttgaagca cggcatcccc cagtcctctt
      841 ggatgagatt gacggatgaa gtgaagattg acttgagtcg tttcttcaac taaagcgagt
      901 aattctgttt tttccttttc ccgcaaattg ttttccaatt cgatatttgc atcaaagtga
      961 tcttcaatgg gtcttttttc ttttcccgtt acgatcaaga tgtcttcgat tcccgaatcc
     1021 aatgcttctt ccacaataaa ctggatcgtt ggtttatcca cgattggaag catttctttt
     1081 gccatggctt ttgttgccgg caaaaaacgt gttcctagcc cagcggcagg aatgactgct
     1141 tttttgacct tcacttgctg ttcctcctaa acgacgatta actattgatc atctatttaa
     1201 cgaagagaaa attcattctc catttttccg tcacgtaaca tgatttcttt tgctgctttt
     1261 ttgatatctt ctccattata caaaacatta tagattgttt gagtaattgg catctcaaca
     1321 cctagctgtt gagctagctc tactgctgct tttgtcgttg ccacaccctc aacgaccatt
     1381 cccatgtttt ctagaacttc ttccagctta tgtccctgtc ctaacaagtt accggcacgc
     1441 caattgcgag aatgaacact tgtacaagta acgatcaagt cccccacacc actcaaaccg
     1501 ataaatgtca gaggattggc acccatcgcg acaccaagac ggctgatttc tgccaatcct
     1561 cgagtcatga ttgctgcttt tgcatcatct ccgaatccta atccatggat agcgccggca
     1621 ccaattgcaa tgatattttt cagtgctgca ccagtttcta cgccaatcac atcattattt
     1681 gtataaatcc tgaaataatc attcatgaac agtttttgta cataggctgc tgctttttga
     1741 tcaatacttg cagctgtaat tgttgtgata tcatgtactg ccacttcctc agcatggcta
     1801 ggaccagata atacaacaat tgcctgtctt ttttcttctg gaatttcttc agccaaaact
     1861 tctgagattc gtttatgcgt atcttgttct agtcctttac ttgcatgtat aatgattggc
     1921 ttagtattca ttttaccaat aagttcctgt gcgactgaac gaatcgcttt tgttggaaca
     1981 acaaataaaa tagcatctgc gttatccact gcttctgcca attctttata tccgataatc
     2041 gattctggga tcgtcaaatc aggtaaaaaa tgctgatttg tatgatactt attgatttca
     2101 tcaatctgtt caggtacatt tccccaaata cggacatcat gcccgttttc tgccaatgtt
     2161 tgtgctaaag cagtgcccca agatccagga ccaagaactg cgattttttg tttcatggat
     2221 attcctccaa ataaataatt gataatttcc attatagtat acatttctta aagttgggac
     2281 taatttccat tgtttttaag tgtttgttta cttcttttat cgtttttact tgaaataatc
     2341 aatgatatgt tacaatagtc tacattttat ttttacactc gttatgcttt gcttgttcct
     2401 aagaggaagc agttttgaca atgttactac cagcattact attaggaggt taggaaactt
     2461 ctcctagtga gtattgattt aaaatacata agagacagat tgaacatttc taaatctgtc
     2521 aggtcctgca acggacagat tgtctctgtt gacggttgac ataggaactt ttgttttagt
     2581 caacattctg gatacaaaac gctatgtaaa ctggcgcaac tcgttgtact tggactttta
     2641 ctacggatcc atgcaacaac taaatagtac tattgcataa caaaaaatgg ttggcaccca
     2701 catgtcccat taaacagatt ataagttgaa ctgtagataa tgcaaaaaat gtgttttata
     2761 acgtaattga tgccaatatt ttaaaaaaat aaagggagca gttatcgctg ctctctttat
     2821 tttttatatt ctttttatcc agtctatcct aataatcaaa ctgacgatag tagcggcgta
     2881 tctctaatgg atggcagttc aactgctcca catgagcatc gatgcgttgt gtcactacat
     2941 gcatcaataa gaaagacaaa acacgtccgc gatatttttc actgatacct tctactggat
     3001 agtcttttgt gtctatcaat gtataattgc tgcaaatttt tggcaaaagt ttagccacac
     3061 gttcagataa cactctctgt tcatcttcgc ctaaaaataa tgtgactggg gttgtttctt
     3121 ctacgatctc taacgtcccg tgaagaaact cggcagcatg gatagatttc gatggaagcc
     3181 agctttgttc ttcccagtaa cacatggcat atgaataaac agctccccat tgattccctg
     3241 ctccaataaa ataatgcata ctatcacgtc gatgtttttc tgcaaatgat aagccaaatg
     3301 catctgcttt tttctctgct tccactaacc ctgttggtaa ataacgttcg agctgttcat
     3361 agtattctga ataagcttcg aattctccat tcaaatacat caatcgatct gctgccataa
     3421 aaaatttgat ttgttctgtt ccaggagctg gataagtgac cacaaaatcg catagctgag
     3481 acaacttact gttcgcttta tcgataaatc caatcagtgt cgctcctact ttcttgattt
     3541 gttctactgc tttcactact tcttgagttg taccggtaac agaggacaaa ataacgatag
     3601 aatcttttgt taatcttttg tttccagtag tgtaatattc tgcagcatgc tgtacaaaga
     3661 caggcaagtt gcttttccca ttcatataag tgacagcttg catcgaggaa gcatacgttc
     3721 caccgattcc tagataatag acagcgtcaa atttttccgc ataaagttta tcgatgattt
     3781 cttctacttg cggacgtaaa gctagtgcgc cttttattgc tttgatttgt tcttcttcat
     3841 tgaatttcag catgatttcc acctcttata atttactttt caagactgta tcaaagtctt
     3901 caaaaatctt tttgttcaag tcaatacctt tagctactgc ggttttataa gcgaatagtt
     3961 gaaacggaac gacaagcgct aatacagaca gatattcatc acattctatt gctaatccaa
     4021 gtgttttctc gtttcttgct ttttttgtgg taatggtgag agtctctcca acgtaccgac
     4081 tcatatatct ttgcaacttt tgcgaacgtt ccttatttac actttcccct tcaataaaga
     4141 atagcaaatg cgaagcatct gcctccaggt atgggccgtg catataagct tctaactcaa
     4201 atccttgtga cggaacgcgg attgtttcag tcaatttcgt ctctgcttct ttagctgttc
     4261 cccagtttgg accatatcca atggtgacga aacgctgtgc taaacgaaat aaactttgat
     4321 atccatcaaa aaattcttcc gttttttgaa taatcgctgg taaatggttg attattttgc
     4381 gcaactgttg catgtagtct tgttctattt ttttgctgat tttattttta cttattccaa
     4441 ttcctatccc taagagtagt aactgtaaaa cagtcgcaga atagcctttt gtaacgaagc
     4501 ctactgattc taccccggca cctaaatcaa taatttgtgc agcttctaaa gcaagtggac
     4561 tttgcacgtc atttgtgata gcaacaacag gtaacccttg tttttgtatc atttttactg
     4621 cttcaacagt agaagcactt ttcccgcttt gtgacacagc aatcacagta tcgattgaag
     4681 gagaaagttt tccataattg aaaaaataaa aaggttcttg aatatcgaca gtaagatcac
     4741 cataagattc caatgctggc ttcgctgcta aacaagcgtt ataagatgaa cccgttgcta
     4801 agatcagtaa attcactgtt tttttcatat gttctcttgt cgaaaaatca ttctttttca
     4861 agatcgtact tagtacttcc ttttcttcat agatataatc ttgcatcgta aacattttct
     4921 ctgctccttt tttaaaaaat ccctatccac gaaccaaaaa ttccaaagaa agctagtccg
     4981 atcaagatcg ttaatggttt cacttctttc ttcaatagat aaaacactat gccaaatgct
     5041 ccaagacata agatatttgg aacgatatca tcaaaaatac tttgaacagt cacagcattt
     5101 tctcctgaac cgatttttaa aggaatattg atggtgatca tcgtggcagt cattgctcca
     5161 acgaccatca gtccaataat agacgcgcct aatgtcaaac tttccatcat accgtttgcc
     5221 tggatctttt tcaaaaaacc cgttcctaag cgatatcccc aacctgttgc tagatagcga
     5281 aacagaatgt gcggaatatt gaaaatcaaa atgaacaaga tagggcctaa aatatttcct
     5341 tgcatggcta aagaggtacc cacacctgtg gcaatcaatc gcaaagttcc ccaaaagaaa
     5401 gaatctccta accctgcaag aggtcccatc aaagatgttt tgatactatc gatcgcagtc
     5461 acatcaaatt caggatcatc cttattttct tcctccattg cagccgtgat tcctaaaatc
     5521 agtgtaacga tatgtggggt cgtgttaaaa aactctagat gccgtttcaa agctgctgcc
     5581 atttcttctt tcgactgata taactttttc aaaatcggaa tcattgcata aacataagcc
     5641 atattcatct gacgttcata attccaagaa aactccattt gaaaagaacg ccaaaatacg
     5701 tgatttagct cttttttcgt tattttgctg tttttgatct cagaattctt catcttcttc
     5761 tacctcccct gcagttgttt gaagtggtag ttgacggaca ttcataatat tgactaccac
     5821 aactgctaaa atcgcaccaa aaatagctac tccggtcaca ggaatctcta agtaagaagc
     5881 tatagcaaaa cctaagaaaa aatatggtgc aacttgtttg ttgatcagca aacgagcaag
     5941 catcgcaaaa ccaagagctg gaatcaaccc tgtagctact gacaacccct tttgaacgaa
     6001 gtcaggaatc atatttaata gatttccgat cgtattgctt cccaccatat aagaaaccat
     6061 taccacaaca ccaagcatca aggaaagacc gataccggca agcaagtgca tatgctcgac
     6121 tcctttataa tttccttctt ctgcataatt gtctgcttta tggttcataa ttggaatcaa
     6181 aagccctaag taaatatttt tcaatatcaa tgtcaacgta gcaatcggca aacctagtaa
     6241 aagcgcggtt tctgtaccgg ctccagctgt aatagcaaaa gccgtcccta aaatcccacc
     6301 ggtcacaaca tcaggtggaa tcgaagcacc tactgagaac gatccgataa atgctaactc
     6361 taaagttgcg cccataatga tcccagtttt tacatcacct aacacgattc ctgtaaacag
     6421 ccccgtcaca atcggacgag aaagcaacga tgtccctagt gcatattctg attgtgcgac
     6481 aaatgcaaca attcctaata agactgcttg tacaatcatt gaggttcttc ctttcttaca
     6541 aaacttctct tacatccact tttttatctg ctggtacttg acggatttct acttctattt
     6601 gtttgtcaat taatcgtttc aagcgttcct cctcttctgg taaaatattt accgcttttc
     6661 cgatgctgcg agtaccaggt tttgccttga tccctcctaa gttgatggtt ttgatttctg
     6721 gatacgcttc tgccagctta tatgcatcct cgatcgattc cactacgatg aataacttat
     6781 atttatctgt caccccgctt ttcaacgcgg caatcgagtc atcaattgat ttgatcacta
     6841 gtttgactcc ttgtggcttg gctaatttga tcgttgtctt tcgtatctca ttcgtaggta
     6901 catcatcatt agcaatcaaa atacagtctg cgcctattgt ttgtgtccat gaaaaagcta
     6961 cttgtccgtg taataaccga tggtcgactc ttgtcaatac aatcattttt cttcctccta
     7021 aaattcgtct tcttctattt tctgattgag ccgctcgtta caaaactgga tcattttttt
     7081 tgaatcagtt agcacctgct ggatcaaaac agttgtctct acttcagcgt ccaaccgaga
     7141 tactagctca atcaatagtg gcaaattcaa tccggcaacg agatagacat gatcccgatt
     7201 gatatattgt aagaattcgt tgttgacact tccgccaaaa agatccgtca gaacaatgat
     7261 ttctttatct ttgtacctca taaaaagctg ccttactgtt tccgctaaat caaaattctc
     7321 atcagtatag caatcgaata cagtaataga atgatttttc ccatagatca gctctaatga
     7381 atctaatatc cctgaagcaa attttccatg ggaagctaag ataatttctc tatccacttt
     7441 ttcatctcct cttttcactt acacatacta aaaaagcaag ttgcatgcca aaattagtgg
     7501 caacaacttg cttatgttca aaattcttct tctatcgaga gaaactctgt atcttcactc
     7561 aaaatatcat agatataggc aatctcagaa ttggggatca tgacactata atgctcttca
     7621 atgacactaa acgcagattt aatagcagat aacccttctt tttgacactg cttccatttc
     7681 tctaatcctt gataggtttc gattggcatt tgtcggatca atcgttcaat gagacagctc
     7741 acatggacat acaacgctaa ttttttggga ttgctgatgc ttcgattcaa ctgcacctct
     7801 aattctctca taaaatgatc gatttctccc atcaccttct ctgtatctag aatagtcacg
     7861 gaatccagta ctttttccaa tgaaaaatta cgaatgatgc gctgattgaa tatttccttc
     7921 tctgaagcat tcatagtagt gtccaaccat tcagctagta cagacatctt ttcccccgag
     7981 atgaggtctt ctaatgaaag atagtcgata ttggtgatat caggatctgt ggtgccaatc
     8041 attcctacga cttcgtatat ggaaaataat gggtcactgt ttttcttatc ttttaattgc
     8101 tgatattcat atgggatgat tttcaactga caagccgtag gtaagctttt ttctagcagt
     8161 tcacttaagt ggaccgccgt accgattcca gtgaagcaag tagtaatgag tgctttgggt
     8221 ttgttttcct ctggatagag cagtcgccat tctggctgga tctgtcgaac agtctcttct
     8281 gctatctctt ctaatgatac atttttttgc aggcattccc ctactgtgat tgctaaaggt
     8341 gtcgtcacat tattcaaaat caaaagcggt acggataact gttttttaaa aaactgatgg
     8401 atttctttca gagatcccat gtctactaat atgatcaatc catttgaaac atcgctgttt
     8461 tcgcaataat caaggatttc tgaagcaatc tgctgtggtg tgacatctag tggcatatca
     8521 aatgattcaa atagatcctc ttgtaataaa cgatttacta catttgccat actgctagca
     8581 gttgcataac cgtgagcagc aatcatcgct tttggcagtc gtttttcttt tgaccatttc
     8641 gcttttttta gatagatcgt cagtaaaatc cgatccatac ttgtgtcttc aatatctaat
     8701 ttgatcttac ttaattccag aattcgttgg acatagtgat aactagatgg ataagactga
     8761 gaaacttgtt gttctaattc tttgattagt tggataatat catgttcttc cggtaaccat
     8821 ttgatagtgc ttcgctgata gagatagtaa ctcaaggcat agacactgtt tccatcgaat
     8881 ttgatttgat aggcagtttc catttgtcga aaggtctctc ggatagattg tgtccaataa
     8941 accagcagct cgtgtttttc ttgtctactg gtctcaaaca gcaaaaaatc aaataattga
     9001 atcacttctt gctttaattt tccttcgcaa gaatatagca gacagttatt tttcttgaaa
     9061 tcaattagga tttgctcaaa acttttaacg attcgctgct gttcgggctg ttgcttctcc
     9121 attaattgct ctaaggtctg gtcttgcgta atatagattc cttgctgtgc tgtattttta
     9181 gtaataggct gcgcaagtag ttctttttgc aaatgatgga tcgtgacatg gatttcaggc
     9241 tgttcacgct gttcagcata tgcttttgct gctgaaactt tgacgatatt tttcagctct
     9301 cctatgtttc cggataattt ctgacttgtt aaaagtgaca aagctggtcc actgactgac
     9361 aaagggctcc ccatttttct ttgttcttcg agaaaaaaag agtaaatcag ttctagccgt
     9421 tcattacgac tacgctgatt aagtgcaggt agttcgattc ggataggtat tcttcgaatg
     9481 aaagttgtta agaaatgact agtaatctct tcagtcgtag caaaaaagag acgtgttttg
     9541 atccggcgtg acaaattagg ttctcccata cgataaatca caccttgatc aagataggtg
     9601 aacagttttt cctgaccttc tggtgttaag cgatgtacct cgtctaaaaa caagatccca
     9661 ccatctgctg cttcaaaagc ccctttttta ttttcttctg caccagtgta cgctccctta
     9721 acatgtccaa acaaattgct agtcaaaagt tcaggattat ctgcatattg tgcacaatta
     9781 aaagtgatga atggtgcaga atcttctagc agatcatgaa gcagacaata ctgatagacc
     9841 aaattaacta aataactttt acctgtgcca ctttcgcctg taatcaaaag tggtaatccg
     9901 ccatctggat agtaaagagc tgttttgatt tgttcgatac tttctttcaa actggcgtcg
     9961 tatccgatca taaaagagaa taagtctttt ggttgcgcga aaaaaggctg ttcctctttg
    10021 agatcatcga tagaaaaata aaaattcgtg cgtaacttaa aaaattgttt ttcaaaagct
    10081 tctttgtgga aataaatcac tggtcgagaa ttgattttga ctagtagttg ctcatcattt
    10141 aactggttca aataatgact gactgtgttt ctttttaccc caaaatgatc agctaacctt
    10201 tgtgctgtaa agacctcgtt catatgttct aaatcgaaaa aggctgtctg gttctctaag
    10261 taacataaaa tttccttctt caacaactta ttcacccctc ctccatcata gaaaacggat
    10321 tcattttgtc aataaaatct ttaataaaga attgaaaaat atatcttatt atagacagca
    10381 gcctacttat cccttataat ttttattcta aaaaacgtac agttccttcc agtttttata
    10441 ctggaatttc aacatatgaa agaataagga ggctatgact attcaatgaa atcggataaa
    10501 tataaaaaca ggaaacatac ttcctggaaa acttttcgag aaatcatcgg atcttatctc
    10561 gctgtagttg tatttttgcc tttgctttat atggctatga cagaacaaac tcgttttatt
    10621 ggcgtagata cgcaatatat agccgaaaca ggatttttat tcatctttat cttgattttt
    10681 gcgttctata aatccttcca tacaaaataa gattcacaga tttcaacttt cctgtctgtt
    10741 tttagctatt acaaatcctt atatgtggta taacaacaat ttattggcat aatgaggaaa
    10801 aacgagttat gatgctttca gagagtaaag aggtactgtt gtcaccatcc tttttattga
    10861 ttacgtacac cactcttagt tttttcccat tgattacgaa aacaaggtag aaacattgat
    10921 gttcctacct tgttttcttt ttttattttc agatcaaaat ctcaagtagc ttttgacttg
    10981 atgtcgggag gttgtcttcg ttccatcaaa cctgatagaa tctgcgccaa accatagttt
    11041 tctccactat tatttattcc tctaagatag tacccacaat aaacgaataa atatttttct
    11101 cattttcata ctccttcttt gaaaatcaat tagatttctc tcaacccact tttttacaaa
    11161 aattacgtag caaaaaagat tgcaagaaca aattaaaaaa gaagtaaata gagtttcatc
    11221 tataaacatt tacttgatat attttttatg cagaattcat ttatagaaaa attaagtaaa
    11281 atagacgttc ctatgaatga agagactggc atgttttctc attttctttt ttttaaaaaa
    11341 aatagtctat tcaatttcta aagcttcttc ataataaaac tttcatttat gaaaaggctt
    11401 gttctctttt cattctaaat ttcctaagct acaatgtggt agtacttgtt ttttcaattg
    11461 tggacaaata aataatagga ggaatcatat gattcagtta ttacgttatg caaaagacta
    11521 tcgtaagcaa atcattttgg gcccgttctt caaatttctt gaagctgtct tcgaacttgt
    11581 cctgccttta atgatggctt ccttgatcga taatggacta aaaatgaatg accgaggaaa
    11641 aatcattgaa atgggcttat ggatggtcgc tatgtctgtg attggtctga tttgtgctat
    11701 catttgccaa tactatgctt cagtagcttc acaaggattt ggtacagagt tacgtaatca
    11761 gctaatcaaa aaaatcaata ctttctctca caacgagttg aatcattttg gaacagatac
    11821 cttgatcacc cgaatgacaa acgacatcaa tcagcttcag ctagctttag caatggtcat
    11881 tcgtttattg atcagagcac cgtttttaag tatcgggtca gtggtcatgg cttttgtgat
    11941 cgactgggaa gtaggtctgt ttttcttagc attattgcct attttctcaa tcattttatt
    12001 ttttatcatc aaaaaaaccg ttcctctcta ccaaaaagtg caagaaaaac tagatcagct
    12061 caatgaacac gttagtcaga atcttagcgg tgttcgtgtc atccgcgcat ttgcgcagac
    12121 aaaaaaagaa acagagaagt tcgataaaag cacaaatgat ctagcgaaca attatttacg
    12181 tgtttccaat atctctgccc tattatcacc tgctacaacc ttgatcatga atgtcgggat
    12241 catctgtttg ctgacagttg gcgggatcaa agtaaatatt ggtagcttgc aacaaggaca
    12301 agttcttgct ttgaccaatt atatgaatca aatgcttttg gcactgatcg ttgtttcaaa
    12361 cctcgtcgtg atcttcacac gtgccgaagc ttcaggaaat cgggtgaaag aggtgctaga
    12421 cacagaaaat tcgattcttg aagctgaggt cagtactact cccgattttt cttctgaagc
    12481 aattatcgct tttgaccatg ttgacttccg ttatactcct gattctggtc tttctctcca
    12541 aaatattaca tttcagctga agcgaaatac agtccttgga atcattggtc caaccggaag
    12601 cgggaaaacg actttgacac aattgattcc gcgtttctat gatgtcagtt ccggtacagt
    12661 tataatggac ggaatggatg ttcgttcttg gcagctggat catctccgtc agcaaattgc
    12721 tcttgttccc caaacttctg tactatttac cgggactatt cgtgaaaacc ttcaatgggg
    12781 taagaaagac gccacagaaa aagaatgttg ggaagctctg aaaatcgctc aggcagaaga
    12841 atttgtccgt cagcttccaa atggtttaga tacaaaagtc atggaaaatg ggaaaaattt
    12901 ctctggcgga caaaaacagc gactgacgat tgcaagagct ttgattgcca agccagcatt
    12961 actgatttta gatgactctt taagtgcgtt ggattatcaa acagacttgg atttgcgtaa
    13021 ggcgctacag acttatcttg gcagtacagt gattattatc tcccaacgtg tccgctctat
    13081 tcaagaagca catcatatct tagtgatgga tcaaggtaag attgctgctc agggtaccca
    13141 tgaagaatta cttgctcaat cagcagaata tcaggaaatc gtcgcatcac aggaggaaga
    13201 atagttatga aagtaaaaaa attatccttg cagacgctcc gccgattcat tccttacctt
    13261 actgcctatg caaaagacat ttggctagct gtgatactag gaatcatcaa tgggattgca
    13321 acagttgtga tgacgttaca aattggtcaa agtatcgatc aaatgattgg agaaggacag
    13381 gtccgcttca atcgactgtt ccatttactt attctattcg gtggtatcgt tttaatcaac
    13441 gtagtcagtc aatggctcat ccaagtgtta agcaatcggt tagcttactt atccgttgca
    13501 aaattaagaa aagatgcatt tgctcatctg aatcgattgc cacttcgtta ttatgatcaa
    13561 aactcccacg gaaatatcgt cagtcgattt acaaatgata tggacaatat ttccattgca
    13621 atttctgcgg ccttcaatca attattttct ggagtggcaa tcatcattct ttcgtttatt
    13681 tttatgatgc gtctaagtcc acttctgaca ttggtcgttc tttgttctac accaattatt
    13741 ttccttgtga gttggatcgt tgctcgtgct tcacaaacaa atttcgatag acagcaaagg
    13801 attattgggg atatctcggg ctttgtgacc gaacgaatcg gtaaccagaa aatcgtgaaa
    13861 gcttttcagc aagaagaagc tagtcaacag caatttgaac tgctaaatgc tgatctatat
    13921 gaaaaaggac aacgggcaca attttcttct tccttgacaa acccgtcttc tcgttttgta
    13981 gatcaccttg cttatttatc gattggttta gttggcggcc tattggcatt gaagagtggt
    14041 tcgtcaatca ctgttggtgt gatttccagc tttacgatct actcaagtca gttttctaaa
    14101 ccatttatcg aactttctgg aatcacgaca caactgcaaa ccgctttttc tggtctagaa
    14161 cgagcatttg atttgatcga tcagccagaa gaaaagcctg atttgccaga tgcctatgcc
    14221 ttggaaccca aaaatatcaa aggaaaagtc tcttttgatc atgtttcttt ttcttatgac
    14281 cccaagcagc gtttgatcga agatttcaat tttactgctt atccaggtca aaccattgct
    14341 atagtaggaa aaacaggggc cggaaaatcc acacttgtta atttgctgat gcgtttttat
    14401 gacacaacaa aaggaacaat tacagtcgat gaccatgaca tccgctctat ccaacgtgat
    14461 acattacgta aaagttttgg aatggtgtta caagatactt ggctatttga cagtacattg
    14521 cgagaaaatc ttcgttttgg gaatccggaa gcaagtgacg cagaaatcaa tgatgcccta
    14581 aaagcctctt atatgtatga atttgtagaa cgtttgccgg aaaaattaga tactaagatt
    14641 ggcggtcaag ggttaaagat atcagatgga caacgccaac tgttgacgat tgctcgaacg
    14701 atgatcagta atccccctat gttgatctta gatgaagcta ccagctcagt cgatacattg
    14761 acagagaaaa aaatccaaca agccttctta gcaatgatgg aaggaaaaac cagttttgtg
    14821 attgcccatc gcctgtccac aatcaaaaat gcggaccaga tattagtgat ggatcaggga
    14881 gctattgtcg aaatgggcac ccatgatgag ctgcttcaaa aagatggtta ttaccgacgg
    14941 ctctatcaag cacaattcac agcacaataa aaacaagcag tcttctggga tgggaagact
    15001 gcttgtttt
//
LOCUS       DACEXQ010000039        14846 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.039, whole genome shotgun sequence.
ACCESSION   DACEXQ010000039 DACEXQ010000000
VERSION     DACEXQ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14846
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.039"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(77..149)
                     /locus_tag="IXH04_000942"
     tRNA            complement(77..149)
                     /locus_tag="IXH04_000942"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Lys,seq:ctt)
     gene            530..1099
                     /locus_tag="IXH04_000943"
     CDS             530..1099
                     /locus_tag="IXH04_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAQ1851571.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1086..1946
                     /locus_tag="IXH04_000944"
     CDS             1086..1946
                     /locus_tag="IXH04_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1851572.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2136..2840
                     /locus_tag="IXH04_000945"
     CDS             2136..2840
                     /locus_tag="IXH04_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1851573.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2845..4680
                     /gene="walK"
                     /locus_tag="IXH04_000946"
     CDS             2845..4680
                     /gene="walK"
                     /locus_tag="IXH04_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAQ1851574.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4677..5993
                     /locus_tag="IXH04_000947"
     CDS             4677..5993
                     /locus_tag="IXH04_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851575.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5994..6863
                     /locus_tag="IXH04_000948"
     CDS             5994..6863
                     /locus_tag="IXH04_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851576.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6918..7727
                     /locus_tag="IXH04_000949"
     CDS             6918..7727
                     /locus_tag="IXH04_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ1851577.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7830..8459)
                     /locus_tag="IXH04_000950"
     CDS             complement(7830..8459)
                     /locus_tag="IXH04_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HAQ1851578.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8629..9921
                     /locus_tag="IXH04_000951"
     CDS             8629..9921
                     /locus_tag="IXH04_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1851579.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9997..10659)
                     /locus_tag="IXH04_000952"
     CDS             complement(9997..10659)
                     /locus_tag="IXH04_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ1851580.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10884..11168
                     /locus_tag="IXH04_000953"
     CDS             10884..11168
                     /locus_tag="IXH04_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAQ1851581.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11219..12844
                     /gene="groL"
                     /locus_tag="IXH04_000954"
     CDS             11219..12844
                     /gene="groL"
                     /locus_tag="IXH04_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAQ1851582.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(12933..14081)
                     /locus_tag="IXH04_000955"
     CDS             complement(12933..14081)
                     /locus_tag="IXH04_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1851583.1"
                     /translation="MLKVWVEQTKDGQFKFIERYVDPYTEKTRKKSTTLTSNSPQAWK
                     KAQKILDKKIKEALENYNKSDITFGELYKEWYEYYKQHVKRTSYLKVPMMMKHVSKHI
                     SDDTIVRNIDETLINKMIEDMYTFGDLSLNYTKQTKTTLSVMLNYAIDRKYIQRNPAL
                     AVKIHPKKVEEEKRKLSMDKKYLEKEEIDQILKQLYSNPRRKLHGIIAEFLYLTGLRY
                     GELLALQMKDYEDGKISINGTLDYTSVKMDNAIKTTPKNTYSQREVQLPNRAKELIES
                     VIADNILAGRPTDPDQYIFISTSGTPLTLHSFNAILHKVEEELELEKSLSSHIFRHSH
                     VSLLSELGVPLKAIMERVGHSDANTTLSIYNHVTKRAKQQVIDKLNSL"
     gene            complement(14183..14767)
                     /locus_tag="IXH04_000956"
     CDS             complement(14183..14767)
                     /locus_tag="IXH04_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HAQ1851584.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
BASE COUNT         4810 a         2570 c         3109 g         4357 t
ORIGIN      
        1 taaaaaaaga acagcgtaaa tgttttcgaa atgctttcgc accaacattt acctgttccg
       61 tgggtttggc acccaatggg ccgtgagggg ctcgaacccg cgacccgctg attaagagtc
      121 agctgctcta ccaactgagc taacggcccg tacaaaaagt acttaataat agtagcacta
      181 agaattttaa aatgcaagac tacatttaaa gaaaatcaga aaaatcttta cttccttata
      241 ggaaatcatt catagattat tcacatttgc tcgctattat ataagcatac caacaaacaa
      301 agttcttgca agagaacaaa ccctaacact tttttcattt tttactcctt tcccgccgtc
      361 tccccggcgg gtctcctgtt cttaacgata aaagacaaga aggcttagcc ttcttgtttt
      421 ttgttttcat ggaatcccgc taatcaaatc acctagttaa agcaactaga ttgaatgttt
      481 atcataagtc tgatatcatg tttatgaaat gaaaaaagga gcggttatga tggaaaaaat
      541 gcgtcagcaa ttaatggagt gggggaagga actagaaaca ttccgtctgc ctcactggga
      601 agaactccct gacttagaac tttatatgga ccaagtcaga acgttagttg atcgttatct
      661 ttcacctgtg atacaaggag aaaagcatcc attgcttacc tcttctatgg taaacaatta
      721 cgttaaacta ggtttgatac cagcaccagt taaaaaaaga tataacaaag aacacgtagc
      781 atttttactt gcaatcacta cgttgaaaca agtattgact attcctgaaa tcaaagaagg
      841 gatattgttc caaggaaaaa cagtaggaat tcgtgaagcc tataatctat tttgcgatga
      901 acaagaagca gctgtgtgga tggtcagcca attagcacaa ggtaaatctc atccgcaaaa
      961 attcgaccag gcaacccctg tagaatatat tgctgtaaga gcggcaactt tatcgtttgc
     1021 aatgaaactt cttgcggaaa aaacgattgt tctagaaacc gaatatttaa aagaggagaa
     1081 aacaaatgaa aaacaataaa atcgcaattt tagtagattc aggtacggat gtgccagaag
     1141 aattgataaa aaaatacgag atgtatatga tcccattgaa aattatttat aaagatcagg
     1201 tatatacaga taaaattgac attacctctg aagagatcta tcaacgtttg ccagaggaaa
     1261 tccctggtac atctttgccg gatggtgagt cgatcacaga aatctttgag caaatcaaaa
     1321 aagacggtta tgaaaaggta cttgcggtaa caatttccag tggattgagc gggacatata
     1381 atgtggttcg tttaattgca caacagcaag aagaattgga agcatatgta ctagatacga
     1441 aaaatattgg tattggtgca ggtttttctg cgatccaagc agcaaaatgg ctggaagaag
     1501 gagtagaatg ggatcaactg atcagtaatc tgaatgagtt agtcgaaaga acaaaagttt
     1561 tctttaatgt tgcgacattg gaatatcttc aaaaaggtgg acggatcggt ttagttgctt
     1621 ctattgtagg aaccgcttta aagcttaacc caatcatctc atgtaatgaa gaagggattt
     1681 attacacagt aggaaaagct aggggacgta aaaaaagttt ggataaaacg gttgcttacg
     1741 tcaaagaaaa aatcggtaat gcaaaggtct tcaacttagc tgttgcccat ggggatgcga
     1801 aagaagaagc aattgaaatg gaagcgagac ttaaaagaga gttcccacaa gcaaaccaaa
     1861 tctactttgg ccagatttct ccggcattag tagtccacac tggtccggga ttattgggtg
     1921 tcggtgtaca agttttagaa ggataaatca aaaaaaggaa acactgaggt gaaaatcagt
     1981 gttttccttt tttattgaag ttagaacaaa aaataataaa gtattttgca ctttcatttt
     2041 aattttctct tttgttttat aggcaaagat gaaacaaaag cagaaatacg gtataatgag
     2101 aaggtatgac aataatatat aagaggtggc tctttatgaa aaaagtttta gttgtcgatg
     2161 atgagaagcc gatttcggat atcgtcaaat tcaatttagc caaagaaggc tatgatgttt
     2221 atactgcata tgatggggaa gaagccttgg aaaaagttgc tgaggtagaa cctgatttga
     2281 ttttacttga tttgatgcta cctaaaatgg acggcttaga agttgctcgt gaggtacgca
     2341 aaacttatga tatgccgatc attatggtaa cagccaaaga ttctgaaatt gataaagtcc
     2401 taggcttaga gttaggcgcc gatgattacg tgaccaagcc attttccaac cgagaattag
     2461 ttgcacgtgt caaagcaaat cttcgtcgcg gtgccacggc tgcgaaggaa ccagaagagg
     2521 cagcacctgc ggaattgacg attggtgatt tgacgatcca tccagaagct tacatggtga
     2581 cgaaacgagg agaaacaatc gagttaacac atcgtgaatt cgaactactc ttttacttgg
     2641 cgaagcacct tggacaagtg atgacaagag aacatttatt acaaacggtt tggggttatg
     2701 attattttgg agatgtacga acagtggatg taactgtacg tcgtttgcgt gaaaagatcg
     2761 aagataatcc aagccatcca aattatttag tgacacgtcg tggtgtcggt tactatttgc
     2821 gtaacccaga acaggagtaa ttggatgaaa agaaaagtcc gcttttttcg atcagtcaat
     2881 tttaagatcg ctattacctt tattctgatt ttattgatct cgatcgaaat tattggtgcc
     2941 tactttatac gtggtttaga acgttccacg atcaatacat ttattaaaga tatgaatcaa
     3001 acggtggaat cattagcaac aacaatcagc ccggaattga atcgaaaaga taatgcagat
     3061 gatgaagagg taaatgcgaa tatcaaaaga tttattgaaa acagtgcgac tagcgatatc
     3121 atcgaaatac gggtcgttga cgaaaaaggg atcatccgag gaacaaccga tgtcaatgaa
     3181 caaagcgctg tgggaaagaa aaatgattat gttgatatca atgatttcac gactaaacgt
     3241 tatgttgctt tagataacga cgataaacgg gtgaatatca atgttcaacc gatcctgtcc
     3301 ccgaccggcg atgccgttat cggggctctt tacgtcaaaa gtaatatcga acaaaaatat
     3361 tcggaaatca ataacacagc agtcattttc tttactgcct cactaattgc ggcatttatt
     3421 tcaatgatcg tatctgtttt agttgctcgt tcgattacac agccgatcgg tgaaatgaga
     3481 gaacaagcca ttcgaatcgc tagaggagac tacagtcgga aagtcaaagt gcatggacaa
     3541 gatgaacttg gacagctggc tgataccttc aatcagttag ctgaacgtat tgaagaagct
     3601 caagatacaa tggaagctga acgcaatcgt ttggatagcg tgctatccca tatgacagac
     3661 ggcgtcattg cgacagatcg caggggtaaa gtcattacga tcaatgatat ggctgtttcc
     3721 ttgctagata tcaaaaacga ggaagcaatc ggacaatcga ttttgacact tttagatatc
     3781 gaagaagaat atacgttgcg gaaattatta gaagatccaa acgaaatggt acttgaccgt
     3841 tcaaccagta cattggaaag tgatcagatg atcttgcgag tagacttttc tatgatccgt
     3901 cgcgaatcag gttttatcag cggattagta gctgtcttgc atgatgtgac agaacaagaa
     3961 aaaacagaac gggaacgtcg tgaattcgtc tctaatgttt cccatgaact tcgtacaccg
     4021 ttgacaagta tgcgtagtta tatcgaagca ttgagcgaag gtgcgtggaa agacgaggaa
     4081 gtagcaccaa attttttgaa ggtaacgcta gatgaaacag accgaatgat tcgaatgatc
     4141 aatgacctct taaatctatc tcgcatggat acaggtaata cccagcttca attagaatat
     4201 gtcaatttca acgagttagt gaattttgtt cttgaccggt ttgatatgat ggtgggaaat
     4261 caggaaaaaa attataagat ccgtcgtgaa tttacgcaaa gagatctttg ggtcgaagta
     4321 gatacagata aaatcatcca agtagtcgat aatatcatga ataacgcgat caagtattca
     4381 ccagatgggg gaacgattac ttgtcgctta ttagaaacgc ataataacgt ggttttaagt
     4441 atcaccgatc aaggattggg gattcctaaa aaagatctga accgagtgtt cgagcggttc
     4501 tatcgagtag ataaagcacg tgcaagagca caaggtggga caggacttgg attagctatt
     4561 tctagagaag tgattaaggc gcatcgaggc gctatttggg cagagagcaa ggaaggaaaa
     4621 gggtcgactt tttatatctc gctgccatat gaaccttatg aggaggaatg gtgggaatga
     4681 gaatctctga aaaagtcgtt cgtataggac tcattttgat ggttgcgtta agcatatact
     4741 tctcttatgc aatctggctg agcccagcag gaaaaagttc aatcaacgta gatgagacaa
     4801 acactcaaat gatcgagtct caaagcaatc gaaaaacttc cgaagtcttt cttccgcttc
     4861 atgttacatg gcaccattcg ggaatgatcc aagaaaccaa cagtgaaaat ctcgtgtacc
     4921 gattgcaatt gatcatcgat aaagcaagat ttggaaagct ttctgaagta gtttcaggag
     4981 acaaatcaca gtttgaacag gtgaaaaatc tgaactctgg ttttgagttg gcctataatg
     5041 cgcctttttt gctaagagaa tacaaggaag catttgattt agatttagat gtgtcgaact
     5101 tggcaaaaga aagcgaaaat atgtatttta ctagaataca attcgatcaa ggaaataaaa
     5161 aaatccgatt tttgaattat tcgaaccatc tcgtatatga agcaaatatt tcgttgaatt
     5221 ggacagatat caataaagaa ttaacagcaa aagacacaaa atggacagaa atgacaacag
     5281 aaccatcgac gatcgacacg caatatgcta cgaaacattc gatcaaactg aaaaagtata
     5341 gttatatcct gtcacagcaa ccctatacac tttttagaaa tgcttttttc cagaaaccag
     5401 atagcgtgaa aaataatgat gaaagcaatg aattgatttt ttacggtggg aatgaaacaa
     5461 tgtctatcca ttcggaaacg caagtagttg attttcgtgg agagctgcca gagaaaaaag
     5521 aaaatgctag tgtattttct gaaagtttcc cctatattag caaattaggc agttctttag
     5581 ggaacctcag atattttgac cgcagtgaca atacgatcga ttatcggata ttcgtggaag
     5641 gcttcccggt ttttgggaca gacagcagag gacagattgg tgtggaagtt ggcggtgaaa
     5701 cgaatggttc tgtgcaagtg aatatccaaa ccagtttgaa tacgattcaa gtgccgatac
     5761 cttcagacga agaagtagaa cttgcaagta cgaatgaaat catccagcag ttaaaagcta
     5821 agggagcaga tagcaacaag attggttcag tgatcgttgg ctatacatgg caaaatgtca
     5881 aagaaacgaa tcgtgtcgtt gatctcttac ctgaatggta tgtgaaatat aaagataact
     5941 ggtattcagc taacgacctt ctggaaaaat tacctggatt ggaggcgaat taattggatt
     6001 tcaaaaaaat cgaatggata tttttcgtcg cttttttagg gttgaatgtg tttttattca
     6061 gcagttatca tgacgcggta taccaagaaa acaatgtcat tcgctctaat cagactgaat
     6121 caatccaaca acgtctggct agcgacgata tcacttacac ggggaagatt tcgtctgaga
     6181 acaagcaagg ctattatctt agcgcggagc agaccaatct tagcacttca ttagcgaatt
     6241 atcgcaaggc tggtaaaagc ggcttgctgg caagtggtgt gtttttagac ggtgatgttt
     6301 tgacgaaatc tttatccagt tcagatgctg aaaagaacgt gatcgatcca gataaaatta
     6361 gtagttcctt ggaagatttt ttatctaaca aagacaatat tttattcggt aatgattatg
     6421 tgtacacgaa aaacttttct catttagaag aagaaccact agaaatccaa gcaagccaag
     6481 agtatgaagg catcccattc aatgatgata cagcaaaaat cgttctttct ttagaaaaaa
     6541 acgataatga ttatagcatc acgaaataca tgcagacgca tttatctgat atcgagcagc
     6601 tgcgcgaaaa gacggaactg tatacggaag aggaagcaat caatacattg tacatcaata
     6661 acaaaatacc aagaggatca aaaattttat ggcgtcagct agcttattcc tgtattctga
     6721 aagtccggga aaaaaatgtg tatgttcctg tttggcatgt agcgatcgaa acaagcgata
     6781 aagtggtcca aattgagagt gtcaacgctt tcagcaatac gatcgtgaca aacaatactg
     6841 taccaaaagt ggaagatcaa taaaaaagcg agtataatga aaaaagttta caaagtaaga
     6901 agggaagtag gagtcaaatg gctgaagaaa aaccagcttt taaaatcagt atcctagcaa
     6961 gtggaagttc gggtaattca ctttatatcg aaagtgaaaa aaaacggctt ttggtcgatg
     7021 caggacttag cggtaaaaaa atcacttcct tgatggctca aattgatcgg acaccggacc
     7081 aattagatgg aatacttgtg acgcatgaac atcgagatca tatacatggc gtgggtgtct
     7141 tggctcgtaa gtatcatttg gatgtttttg ccaatcagca gacttgggaa gcgatggaac
     7201 cactgattgg aaatgtacca gtggaacaaa aacatctatt tgagatgggg aaagtcagaa
     7261 cattcggcga tatcgatatt gagagtttcg gcgtctccca tgacgcagcg gctccgcaat
     7321 tttatcgttt ccataaagaa aatcggtcat tcgtcatgtt gacggacacc ggatattgca
     7381 gtgattatat gcgaggtgtc attgaaaatg ccgatggtta tttgatggaa agtaatcatg
     7441 atcttgagat gttacgcatg ggaccttatc cgtggaattt aaaacagcgt attttaggag
     7501 atagaggaca tctttccaac gaagacggtg cacttgtcat gactgatgtg atcggtaatc
     7561 ggaccaaacg aatctatctt ggccatctta gcaaggaaaa caatatgaaa gaacttgctc
     7621 atttaacaat ggaaaatgtt ttgaaagaaa aagattttgg cgttggacat gattttgaaa
     7681 tctatgatac agatccagat gaagcaactg agctattttc gatttaaaca gttcaatcaa
     7741 taaaaatata aaaagcagaa gataaaatca atcagggttg gttttatctt ctatttttat
     7801 ttttttagca tattaaaaaa ataaccgtat tatcgactga ctacactgat gtgctgcttt
     7861 tcatgtttct catccagtat ctcatctact aatgcaatcg cgtaatctgc catgctgatc
     7921 gtactgtcgc cagcagcgtt tctttgtaaa taatcatcac ttaacaaata agtacctgta
     7981 cgtttgccat gtggatcaaa taaagaagct ggactgatat aagtccaatg tagattttta
     8041 gattgtttca attttaacaa tccttcgtac atattgaaag ctgttggata gaagggtgca
     8101 tttttaggta cttggtcaat catacgagtc gtcttttctg tatctaagaa taacgaagag
     8161 gcacccccaa cgacaattaa gcgtgtatcg gttccttcaa gcatctcaat caaatgggac
     8221 aaagaagttt gatgcatttc ttcttttcct ttgggagcgt tgaaagcatc aacgacaaca
     8281 tcaaaagatg caagatcagt agttgtcaac tcaaataaat ccttttgtat aaacggaaca
     8341 tctgatggca atttttctgg atgacgcacg attgccgtca cgtctaaatc tctggacaat
     8401 gcttcgtcta aaataaatga acctgcatgg ccagtagctc caatgagtgc aattttcata
     8461 aaaaacctcc ttttttattc ctgtccattg taagcagttg ctgaatattc gtcaaagaat
     8521 ccgatttttg aaaagctcag tgcgcattcc tttcatggtt cgtgatcatt gggtatagtt
     8581 aaattaagaa aactgattga taaaaataga agttgaagga gataagaaat gaaagagtgg
     8641 ttagatcgtg cctttttacc aatgataaca tctgttgtga atcttagacc cttaactgct
     8701 ttgcgtaatg gaatgactta cacgatccca ataattattg ttggctcgct gtttttctta
     8761 tttgctcatt ttcctgtgga ttacatcgct cagtggatag acaaaacagg attttctatg
     8821 tattttacac aagtatacca ggctacattt gatgtgatag ccttttggat cgtttttgcc
     8881 attgcatacg cttatgcaaa agaagaagac atcgatcgct taccagtcgg cataacagcc
     8941 ttgtctgctt atctactgct tattcagcca ctaaatactc agatagatac tgtctggaca
     9001 ggtgctgaag ggatactagg agcagttaca actgggctca tcatcggatg gggctatgca
     9061 cgtttagtaa gacttgcgaa agggtggaag caaagagaga agatacctga aaatgtttgg
     9121 cagtctttta cttcattgat tccaatcatt gttatagtca cttttacgct tatcggttca
     9181 gcggtttttc aacagttgtt cagaatgact gttgttcaag gagtatggac atatgtccag
     9241 cttcccattc aatcgttcat agacacgttg tttggtgtgc ttttattagg tttttttgtt
     9301 ccgtttttat ggttatttgg tcttcatgga tctgcgatgg tcaatgggtt ggttagtccc
     9361 gtcttgcaag cgaattcatt agcgaatgca gaaatcttag cctcaggaca agagttgacg
     9421 gtggcaaatg gcggacatat cgttactcag caatttctcg atcagttcat gacggttacg
     9481 ggtgctggat tgacgttagg ggctgtattt tttatgacgg tttttgcgaa atcgaagaaa
     9541 tacagagaat taggaaatct tgctctttta ccagcctttt tcaacatcaa tgaatcaatt
     9601 attttttcca caccgatcgt gatgaacccg atgatggctg ttccttttat tttcgcaccg
     9661 gtcgtttcag gattgattac ctattccgct ttatattttg gattcgttcc tttatttaca
     9721 gcagtccaag tcccttggac aacaccgccg atcctctcgg gtttcttagt aggcggagtg
     9781 cctgcagcga ttttacaagg tatcgtgttg tcaattagtt ttttcattta tttcccattt
     9841 ttgaaaaaaa tagattctgc aaactgcaaa aaggaaagac agacaaaaaa tgatggagca
     9901 gcagaaaaga tgaacgacta aaaaacaaaa atactcctgt cgctttagat tgggaaaatc
     9961 accaatctag gatgacagga gtatttatag tcatgtttat actagccctt tttggacagc
    10021 taattgaaca agaacaacta atgtgttcat tcctacgtgt gtgatcatcg atgtccagat
    10081 ccgaccagtt gctttgtact ctaggctgaa gaagaaacct aggaaaaagt agattagcag
    10141 atgaccatca ttatgagcaa acgcaaacag caaagagctg atcactgctc cgacccaaaa
    10201 atttgaatat cgactgatga tccccagtat actgcggcgg aatacgaact cttccatgat
    10261 gggtccagca acagttgtcg ccaaaataaa agcgggtgct tccatgatca tctggatgat
    10321 attttgtgta ttctcggaag gggttgcttt gccaaacaat acagattcta acatgacagc
    10381 gatgctttgc agaatcaatg cgacaaagat tccaaccaat ccataaataa caatctttgg
    10441 agcagaagct ttttttaccg ctgattcgat cgctaagggt tcacgttgct taaaataaaa
    10501 aatagctagg acaacggcgc ctagtatata catgactgca gttgctgtga tcaattcaga
    10561 cgtagatttt acaagtccta tcgaagctag gatcaagggt gagataaaaa ttagaccata
    10621 gcaaaaaatg ctgaggaagc tatatttttt taaagacatg taattcctcc ttttaaggga
    10681 taacaagcat gcaaggcaag cttatatcga ttgatttcaa ttatatcata atttcaataa
    10741 gcagctgtca gaataaaatt tcttttcttt gatttaaaag cttgcaaaaa agagggagaa
    10801 tgagtattat taaaaatgta ggttagcact cgaaggttaa gagtgctaac agattaaaaa
    10861 aagagttatc ggagggattt atcgtgttaa aaccattagg tgatcgcgtc attatcgaag
    10921 tcgcaaaaga agaagaaaaa actgtcggag gaatcgtttt agcttctgct gctaaagaga
    10981 aaccccaaac tgggacagtt gttgcagtcg gtgaaggccg tttgttagaa aacggtgaaa
    11041 aagttcctgc cgctgtaaaa gcaggcgacc aagttatgtt cgaaaaatac gccggtacag
    11101 aagtaaaata cgaaggaaaa gaatatctga tcgtcgcagg aaaagatatc atggcgattg
    11161 ttgagtaaat ttggaagata aaaaaataat caaaaataat caatgaggtg aatcaatcat
    11221 ggcaaaagaa ttgaaatttg cagaagacgc acgcgcagct atgttacgcg gggtagataa
    11281 attagcagat acagtgaaag taacattagg acctaaaggc cgaaatgtcg tactagaaaa
    11341 atcttacggc tctccattga tcacaaatga tggtgtgacg atcgcaaaag aaatcgaatt
    11401 agaagatcat tttgaaaaca tgggtgcaaa acttgtatca gaagtagcat caaaaacaaa
    11461 tgatattgca ggagacggta caacgacagc aactgttctg acacaagcaa tcgttcgcga
    11521 aggtttaaaa aacgtaactg caggtgctaa tcctctaggc attcgtcgcg ggatcgaatt
    11581 ggcaacaaaa gcagcagtcg aagaattgca taatatctca actgttgttg attcaaaaga
    11641 agccattgca caagtagcag ctgtttcttc gggttctgat aaagttggac atttgattgc
    11701 tgatgcaatg gaaaaagttg gaaacgacgg tgtcatcaca atcgaagaat caaaagggat
    11761 cgaaacagaa ctagatgttg tagaaggtat gcagtttgat cgtggttact tatcacaata
    11821 tatggtaaca gataacgaca aaatggaagc tgttctagaa aacccatata tcttgatcac
    11881 agacaaaaaa atctctaaca tccaagatat tctgccattg ttagaacaaa tcttgcaaca
    11941 atcacgtcca ttgttgatta ttgctgacga tgtagatggt gaagcattgc caactcttgt
    12001 attgaacaaa atccgtggaa cattcaacgt agtagctgta aaagctcctg gtttcggcga
    12061 tcgtcgtaaa gcaatgcttg aagatatcgc tattttgaca ggtggtacag taattactga
    12121 cgatctaggt ttagaattga aagatgtaac aatcgaaaac ttaggaaatg cttctaaagt
    12181 tgttgtagat aaagacaata caacgatcgt agaaggttct ggagaaaaag aagcaatcga
    12241 agctcgtgtg caattgatca aaaaccaaat tgctgaaaca acttctgatt ttgaccgcga
    12301 aaaattacaa gaacgcttag ctaaattagc aggtggtgta gctgttgtta aagttggtgc
    12361 agctactgaa acagaattga aagaattaaa attacgcatt gaagatgcat tgaatgctac
    12421 acgtgccgca gtagaagaag gaatggtctc aggtggtggt acagctctag tgaacgttat
    12481 cagcaaagtt tctgctgtag aagcagaagg agacgtggca acaggtatca agatcgttgt
    12541 tcgtgcttta gaagaaccaa ttcgtcaaat cgctgaaaac gctggttatg aaggatcagt
    12601 gattgttgat aaattgaaga acgtggaact aggcactggt ttcaatgctg ctactggaga
    12661 atgggtaaac atggttgaag caggtatcgt agacccaact aaagtaacac gttctgcctt
    12721 gcaaaatgcc gcttccgttt ctgccttgtt attaacgaca gaagcagtcg ttgcagataa
    12781 accagaacca gcagcgccag cagctcctgc aatggatcca tcaatgatgg gcggtatgat
    12841 gtagtaatta agaaagaacc ctattttaac agggttcttt cttctttttt tgttttattt
    12901 taagcaaaag gggcaaagaa ggggcaaaaa tatcaaaggc tatttagttt atcaattact
    12961 tgttgtttgg ctctttttgt tacatggtta taaatagaca gagttgtgtt tgcatcagaa
    13021 tgccctacac gctccataat ggctttaaga ggtacgccta attcagataa tagtgaaacg
    13081 tggctatgtc tgaatatatg tgaggataga cttttttcta attccaactc ttcctctact
    13141 ttatggagta tagcgttaaa tgagtgcagc gtaagcggag tgccacttgt agatataaat
    13201 atatattgat cgggatctgt gggtctacct gcaagaatat tgtcagctat cacactttca
    13261 atcaattctt ttgcacgatt gggtaattgc acttcacgtt gcgaataagt attttttgga
    13321 gttgttttta tagcattatc cattttcaca gatgtgtaat ctaaggtccc attaatggaa
    13381 attttcccat cctcatagtc cttcatttgc aaagctagca attccccata tctcaaacca
    13441 gtcaaatata gaaattcagc tattatgccg tgtagttttc tgcgaggatt ggaatataac
    13501 tgtttcagta tttgatcaat ttcttctttc tccagatatt ttttatccat agaaagcttt
    13561 cttttttctt cttccacttt tttaggatgg attttaactg ctagagcagg gtttctttga
    13621 atgtattttc tatcaattgc atagtttagc ataacggata gagttgtttt tgtttgcttt
    13681 gtgtagttca gtgagagatc accaaaagtg tacatatctt caatcatctt attaatgagt
    13741 gtctcatcaa tgtttctaac gattgtatca tcgcttatgt gcttagaaac atgtttcatc
    13801 atcattggaa ccttcaaata gctagtacgt ttaacatgct gcttataata ttcataccat
    13861 tctttataca gctcaccaaa agtgatatct gatttattgt aattttcgag tgcttcttta
    13921 atttttttat ctagaatttt ctgagctttt ttccacgctt gtggtgaatt acttgtaagt
    13981 gttgtagatt tttttcgtgt tttttctgta taaggatcta catatctttc aataaacttg
    14041 aattggccat ctttggtttg ttcaacccac acttttaaca tctcctatca tttgctataa
    14101 taggcataac aaatagacct atataggttt gttttctaaa agcacgctct tactctggac
    14161 ggtggggcgt gttttttatt tattatacag ttgctttaaa aatagctgct ttttcaaatg
    14221 atactaaagg agagaagtgg atttctattt ctcccttttg gtttaacgtg aaatgagcag
    14281 ttacatccat ttgttttcct ggagcaacag atcccattgt attttcgttg gcatatgtct
    14341 ctgatttttt gtcatctggt ccatatactt ccacatctgt acctacaggg atatctgaat
    14401 cgccatcatt ttttacggta taagtaattt ttactacttg tgcaggttgg ttttcttcaa
    14461 attgatttct ttcatcagtt agttctacac tatttagtgt atattcagca tcaccaactt
    14521 ttacagtgtc accaatctta tagaaggtat cgcttttttc ttctttagaa gatgaagcag
    14581 aggttgaaga tttagttact ttttcgccac cattatcact tgctttatta ctatctgatc
    14641 caccatttaa agcagaacca ataattataa ttaaaattac tgctaatacc caaaaccata
    14701 cttttttgta aaaaggttta ctaactttat acatttttcc gtcttgaccc ataacttttt
    14761 ttgccattta aatattcctc acttcttgtt ataatatatt tgcgatctca gaaatgaggt
    14821 atgagtccgt gtgccagcac gggctt
//
LOCUS       DACEXQ010000040        14805 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.040, whole genome shotgun sequence.
ACCESSION   DACEXQ010000040 DACEXQ010000000
VERSION     DACEXQ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.040"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(172..1590)
                     /gene="nhaC"
                     /locus_tag="IXH04_000957"
     CDS             complement(172..1590)
                     /gene="nhaC"
                     /locus_tag="IXH04_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAQ1851585.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(1993..3216)
                     /locus_tag="IXH04_000958"
     CDS             complement(1993..3216)
                     /locus_tag="IXH04_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAQ1851586.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(3247..4086)
                     /locus_tag="IXH04_000959"
     CDS             complement(3247..4086)
                     /locus_tag="IXH04_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAQ1851587.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(4103..4783)
                     /locus_tag="IXH04_000960"
     CDS             complement(4103..4783)
                     /locus_tag="IXH04_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1851588.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(4785..5858)
                     /locus_tag="IXH04_000961"
     CDS             complement(4785..5858)
                     /locus_tag="IXH04_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1851589.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGGVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     gene            complement(6505..7176)
                     /locus_tag="IXH04_000962"
     CDS             complement(6505..7176)
                     /locus_tag="IXH04_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1851590.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(7263..9941)
                     /gene="mgtA"
                     /locus_tag="IXH04_000963"
     CDS             complement(7263..9941)
                     /gene="mgtA"
                     /locus_tag="IXH04_000963"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAQ1851591.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            10600..11037
                     /gene="ndk"
                     /locus_tag="IXH04_000964"
     CDS             10600..11037
                     /gene="ndk"
                     /locus_tag="IXH04_000964"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAQ1851592.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(11059..11925)
                     /locus_tag="IXH04_000965"
     CDS             complement(11059..11925)
                     /locus_tag="IXH04_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1851593.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(12085..14178)
                     /locus_tag="IXH04_000966"
     CDS             complement(12085..14178)
                     /locus_tag="IXH04_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ1851594.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
BASE COUNT         4298 a         3162 c         2530 g         4815 t
ORIGIN      
        1 cgagaggttg tgaaaaagtc gcttagtttc gcgtattaag aaaaattttg tgaaatggct
       61 ttccacattt ttgtataaat ttggcttaat accgagaaac tggcttttga ataccgttta
      121 ttcgtaaaag ggattgtgac aaaagtcttg tcacaatccc tttttatgtt tttattgttc
      181 taattgcgct gtcttgctta ctgcttcttg cggtttcaca aatttttttg agattcccca
      241 acctgtgaag gcactgacca tcgaaaacac aggagaaagc aaactgaaaa atacgaatgg
      301 caagtaagtg agtgtaggaa taccaaatgt acttgctacg aaacttccgg caattcccca
      361 aggcactaag tagttgatta ctgttccacc atcttctagc gttctaccta atacagttgg
      421 atcaaatcct gctttttcat aagtttcttt aaatgcatta ccaggcagaa taacagatag
      481 gaattgttca ccaataaaaa tatttactcc gatacttgtc atcaatgttg taaaaatcaa
      541 atttggtgtg ttcgtcatct ttcttgatac tcgttccatc acggttgaga tcaagccaaa
      601 ttcgatcaac aatccaccta atgacaaagt tagaataatc aatgacacag taggcatcat
      661 actttcgatt cctccgcggc taagcagtga atcgatttgt tggtttccag tatttgaaac
      721 aaaccctgat tcgatgaccc cagccaactt cgttaaggaa gtttgtgggt tttgaataaa
      781 aatcattcct actgctacag cgatattcaa caaaatcgtt aaaattgccg gcaccttttt
      841 ccatgcacac agtaacatta aaaataaagg aatcagtgac cagattgaaa tagtaaagtt
      901 tgcttctaga acatcagtca cttcacgaat agatgaaaga cttgcggctt tacttgtgtg
      961 tcctaaaatc atgaataaaa agaaagaaac aacaaatgcc gggacagttg accacatcat
     1021 atgcttgatg tgtttgaaca aatcggcatc aacaatagct gctgctagat ttgttgattc
     1081 ggataatgga gacatcttat ctccaaaaac ggcacctgaa acaatagctc caacaaccag
     1141 cgctggattt aagcctagtg ttgtcccaat accgaaaaat gcaatcccaa tcgttgacat
     1201 aacagtaaag gcactcccta cagcgctacc tacgattgca cacacaacaa atacagaagg
     1261 gacaaaccac tggacactga tcaatttgaa cccaatgacc attagagttg gaatgatccc
     1321 tgcttgtatc catacagcaa tcaatgaacc gactaaaata aaaataaaga ttgggatgat
     1381 tcctggtttg atgccattga tgattccttc gtgcagccaa tctgtcgggt atctttttat
     1441 cactgcatac aacatgacca ttccgatcgc tgctaagatc gtgatattag gtgagacctt
     1501 taatccgaca acgccaatcg caatgcctaa aacaataacc attaatacaa gtgttgcttc
     1561 taaaaaactt ggtgttcctt tttttcccat gataatctct ccttttttag ttgttcttat
     1621 gttctaaaaa agttaagata tcggattact ccgccgcaat tcatccgcga ttcctccttt
     1681 ataaaaataa aaagacctgt tttaaactaa ggacgaatga ctccgcggta ccaccttaat
     1741 taaaaacaga tctgtttttc actttcaaac ttttattcaa atgtatggac tttccatgta
     1801 attcgtcaat taggcttgat cggttcacag cgaccaccga ctttctgaac aaaacaccta
     1861 accgctactg cgtgaaaagt tgacttgtac gaagtttacc ttccataaga tcatttgtca
     1921 atgaaaaaga aaatattttt tcatttttct gaaaattatt tttataactt tcacacacaa
     1981 gtttattttt tactagaaag tagcgccctc ttgttttttt cttgctacta tttcttgatg
     2041 ttcttgcttg attctttcta atagctttgg ctctttcaac agatcaagag ctgtattggc
     2101 gagtagttct gctgcaatcg caatagaagc aaggcctttg tcacttttag cagcagcttt
     2161 gaattcttct gtgtgtccgg caatatatgt ctcagagatt gctaccgtag gctggattgt
     2221 tggtatgact tggctgacat ttcctacatc agaagaaccg agacttgttc tttgttccgt
     2281 ttctatttct tcatctggaa cacctgcttc ttttgcatgc tggaagaata agtcatcaaa
     2341 agaaggagta acgatgacat catcaactgc attttggaac aatccaaact caaaagtcgt
     2401 gcccgtactt atcgcagcac ctcgtacgat attttccact ttttcatata ctttatctaa
     2461 tgtttgccga tttcctgcac gtaaatagaa acgtccagca gcgtaatctg gaatgacgtt
     2521 ggcagctact cctccgtctg taatgatccc atggatattg acatcttttg gcaattgcaa
     2581 gcgtagagcg ttgattccat taaacacttg gatcaatgct tccaaagcat ttactccttt
     2641 ttcaggtgct gctgctgcat gggaagcgac accgtaaaat ttgatgtcga ctggatcatt
     2701 cgctaaagat tctgtcgttt tcccataacg aaaagctgga tggacgcata acgctacatc
     2761 gacatcctca aaaaaacctt ctcgaacaaa gctgccttta gctgatccgt tctcgccccc
     2821 ttcttcccct ggggtgccat atacacggat ttctccacca gtttcatcaa tgaattgctt
     2881 cactatgctg gcggctaata cggagtaagt accaaacaaa ttatgcccgc atgcatgacc
     2941 aatacctggt aaagcatcga attctgccaa aaaggcaatt gtgggaccgg gtttattgga
     3001 aatataacgt gcatcaaagg ctgttcggtg tccagctact tctttttcta cccaaaacct
     3061 ttctttttct aattgtttaa tcaacgctgc tgaggaataa aactcatagt tacttacttc
     3121 aggatgttcg tggatatcta gagcaagtgc ttgatattct ggtaaacgat attggattag
     3181 atccttcaat tggtttgttg agattgttgt tgtcataaaa aattcctcct atgctagtca
     3241 tttttcttat tttctcccga atgtttccca tgcaggcacg cttgatcctt ttgatgtttt
     3301 ctcgattact ttgactgtgt cttctgtttg ataagcgtct actatttttt gatacgtttt
     3361 attgttttca tcttcttttc ttgcaacgat gatattcaca taaggacgtg catggtcatc
     3421 cactggttct aagaagatgg catcagaagc aggattaaat ccagcatcta cagctacacc
     3481 gctattgatc actgaaatgt ctacatctga caaagcacgt gctgtttggg aagcatctaa
     3541 ttcagaaatg tctagatttt ttctattctc ggtaatatca gaaacagtgg gcgcttgtcc
     3601 ttttgcctta tccactttga tcaatccagc agtttgcaat aacagaagag ctctgccgcc
     3661 atttgtaaca tcattaggga ttgctacttt tgctccgtct ttgatatcag ctacatcctt
     3721 caaagtgtta gaataaattc ccagcggtgc attgacagta tttccgatcg atactaaatc
     3781 agttccgtgt tctttattgt aattgtctaa aaagaattga tgttggaaag aattcaaatc
     3841 aatcgatcca tctgctaaag ccacatttgg ttgattgtaa tctgtaaatt tcacgtattc
     3901 caaattaatg ttctcctttt tcaaacggtc tttcacgttg tcccatacat ctgtatcttc
     3961 tccaataatc cctagtttta cgatagtttc ttttttgtca gttgtatctg cttttccttg
     4021 gctacaccct gccaaccaaa taattgctac aaatgtaatg actacaaaac gaataatttt
     4081 tttcattttt tctcctccac tttcaatgtg taattttttt aatcagttga ttactgataa
     4141 attgacttaa aaaaactaag attagaatca ataatgtagc cacaatcgtt acatctgttt
     4201 ggaatcgatt ataccctctc gatatagcaa gattccctaa tccgcctgca cctactgctc
     4261 cagccatggt agtcagtccg atcacgttga tgatagtcaa cgccgagacc cgaatgatac
     4321 caggcaaacc ttctaacaaa tacacacgta aaatgatacc gattggactg gttcccatcg
     4381 cttctgccgc ttcgatcacc ccaggatcaa cttctagcag tgcgttctcg atttgacgag
     4441 caaaaaacgg aacaactcca aaaatcaatg gcactaaagc tgctttcgca ccaatcgtcg
     4501 ttccaactaa gaaacgagta gtcaatgcta aaagagaaag caagataatg aaagggatgg
     4561 aacgaatgat attgatcagc ttatcaagta tttcaaatag cagacggtta gctagtaatc
     4621 cattagggcg tgtcactact aacgtcacac ctaagatgat tccaatcatc ccgccaatga
     4681 atgctgtcca aaaagacata tatagtgttt gcttcgtcgc ttcgacaaat tcatccggaa
     4741 tcaaataaac attgggcaaa tatgttttaa agaaatcagt cattttatct tcctccttcg
     4801 atcactttga tacgactatc taaatcgttt ttttgtaaaa tatttatttt tacaccagat
     4861 ttgatcaaat aatccaatgc ttttttccgc tgtttggttt ctccagctaa agtaacgatc
     4921 aaactcccta acggcgtttc ttgtaataac tctacattgc catataaaat attcgctgtt
     4981 acttgatatt tgctgtacag atgagcgatt agaggttcgt tcgtctgatt tccaatataa
     5041 cttaattcca ccagccattt attggcgatc tgatcggcaa aagcagaatg ggcaatgatc
     5101 gtttctaatg cttggtcaag atgagtagct gttcgaataa aatctttggt caattcttct
     5161 tcaggattgc tgaagatctg gatgctgcta cctttttcaa cgattcttcc atcttccata
     5221 accgctacct tgttgcagat ttctttgaca acttgcatct catgagtgat caggacgatt
     5281 gttaaaccta actggcgatt caattttttt aacaatgcaa ggatctgaag ggtcgttttc
     5341 ggatcaagtg cactggttgc ttcatcacat aataatacct taggttcatt cgccaaagct
     5401 cgtgcgattg ctactcgttg tttttgtccc cccgacagtt gtttaggata agcagatgct
     5461 ttttcttcca aaccgacaag ttccagtagt tcattcactt tttgacgacg ttcctgtttc
     5521 gattttccag aatactttaa agaaaaatca acgttgtcaa aaatagttcg gctttccatt
     5581 agattgaaat gctgaaagat cataccgatg gcctttcttt cttttcgcaa ttctttggga
     5641 gagagctgac tcagattcgt ctgattgaca atgacttctc ctgctgttgg tttctgcagc
     5701 aaattcatca cacgaactaa cgtacttttc cctgcacctg agtaaccgac gattccataa
     5761 acaccgcctt tttcaatcgt taaatctaca gaatctactg cctgtatctc tttttctttt
     5821 tgtttgaaac atacagaaac atctcgcaat tcaatcatta aaattcctcc ttatctaaaa
     5881 aaagcgtcct caagaaaatg aaaatgtcat tttcttgagg acgctttttt gcgtgttacc
     5941 acctcatgtt caagctgtgt tcacacacaa ctcctcttca agtacgatca tactctagca
     6001 agatatcggt cgcagccgtc atcgccttac atctatgctc gggatgaatt gctcgatggc
     6061 cattttccaa tagtcgttcc ttgcttcttc tcagctaccg aagctctctg tcaaaggttc
     6121 ttctatcgta cttaccattt caatgcaaat aatattcaaa aaaaaagtcc ttttactaac
     6181 ataaactatg tcagtaaaag gacgacgtat cgcggtacca ccttttttcg gaagtttcct
     6241 tccctcaagc ccaacatctc aaaaagagag attggcgttc gttttaacgg tcgaacagtt
     6301 ccgaatttgc ctacttatcg acaaattatt ctccaaggcc attttcagct tttcttactt
     6361 gactcttctc accagccaga gcctctctgc aaagtttcca aacttacttt ccttttcaac
     6421 gaaatcgata ttttgttgtt gggattaatc tacatgagga aaactgaaaa gtcaagtacg
     6481 aaattcaaat tattttttct atatttattt ttctgttttt ttatttaaca tttcaataac
     6541 agttcgaaga tcttttcctt ccgcaagaag tttcaatttt tccttttctt cgacggtata
     6601 atttcggaca aaactggaat caggtatcaa aacaacgcct aatcctgctc gtgaagcagc
     6661 tgttgctcct actaaagaat cttccactgc aaaggctgaa ctagcttcta atcctgttcg
     6721 ttccaaagct gtcagataag gaatcggtga aggcttgttc tccgacactt cgtctccgaa
     6781 tgtaaacgtg tccaagtaat cagctaattt aaagtgatcc aagatatctg ttgctctttt
     6841 cgtaacagtg gtcgtagcaa tgcctagttt catatgacgt tcttttaagt ctgccaatat
     6901 ttcttttgca tatggtttta agtggatggc tccgcttgtc agctgttgat aaaaatattc
     6961 ttcacgcttc gctcggactt cctgtacttt ttcagggcta cctgctgctt tcactaaaat
     7021 atcaaaactt tgtttccagt tttttccgcc ccaagcgtca acgatttctt ttgggatatg
     7081 gatatcaaat tgctgaaatc cccaaagcca gccatcacga tacgtttttt ctgtgtcaac
     7141 taacaaacca tctaaatcaa atattgcacc tttcatctat acattaatcc tttccaaaac
     7201 aatacacata gtttagcacg actggtccaa aaagaacaga agcaaagaac cagccttctc
     7261 gtttataaga gttcttggaa tcgacggata tagattttct tcacagctgt cacaagcgcc
     7321 agataagcga tgactgtagc aaataaccat aaccagtaat tgccaggaag tggcatcaag
     7381 cctaattcag caccaaaact tgtaaaagga aggacagaac cgacaataat tcctaatgta
     7441 gtgataacaa acatggcaaa tgttgcgttg ctttggataa atggtacttt cggtgtacgc
     7501 agtgcatgga gcactaaggt ttgtgtccat agtgattcga caaaccatcc tgcatggaat
     7561 aatgcgatga aaatcactcg ctgtgaacta tccaatgtat ggaaacttcc gcccactaca
     7621 gcaggacaaa taacaaaata catcaacaag taagtagtaa tatcaaatat cgaacttgtt
     7681 ggaccaaagt aaaccatgaa ttccccaata ctagatgcat tccatttttt tggctcgtcc
     7741 aaatattctt tatccatccg atcccatggc aaggaaatgc aggaaacatc atagatcaga
     7801 ttcaaaaaca aaagttgtaa aggcagcatt ggtaaaaatg gcaggaacgt acttgcaatc
     7861 agcacggaaa acatattgcc aaaattagaa cttgctgtag ccttgatata tttcatgata
     7921 ttcccaaaag tttctcgtcc agaaagaagg cctctttcca aaatcgtcaa atctttttct
     7981 aaaaggatca cgtctgcaga ttctttcgca atatccacag ctgtatctac agaaataccg
     8041 atatcagcca ctttcattgc aggagcgtca ttgataccat ctcctaaaaa tccgacagta
     8101 tgaccatttt tacgtaaagc tgtcgtcaaa cgtgcttttt gctgaggatt caattttacg
     8161 aatacttcat ggtcttcagc tacttgtgct aattcatgat cactcatttc cgagattttc
     8221 tcaccggtaa ccaattcatt gatctctaga cctacttgac ggcacactga acgtgtaact
     8281 aattcgttgt ccccagtcag aacttttaca gctacaccat gttctttcag agctttcaac
     8341 gcttgtttcg ttgtttcttt tggtggatca aggaaagcaa gataaccaat caaaaccatg
     8401 tctgactcat ctttgactga aaattctcca acgacgcttg gattggtctt ttgtgcgaca
     8461 ccaatcactc gtaagccatc ttcattaagt gactgtactt ttgcaagtac ggcttctttt
     8521 cgttcttttg ttaatggtgt aacagtaccg ccaagatcaa taaatttaga aatgttcagc
     8581 atttcttcaa tcgcgccttt tgtgatcatt tgtgttttac cagctggatc agcaaccaca
     8641 acactcatac gacgacgttc aaaatcaaaa ggaatttcat ccactttttg ataattgatc
     8701 ttatctgtat ctaaagtttc tgttgcttca tcaatgatag ccacatctaa caaatttttc
     8761 agccctgttt gatagtagct atttagatac gcatggcgca aaacacgatc atcttcattt
     8821 ccttcacaat ccatatgata ttcaagaatg attttatctt gtgtcaatgt ccccgtttta
     8881 tccgtacata acacatcgat tgcaccaaag ttctggatgg cattcaaatt tttgatcact
     8941 gttccttttt tggccatcac acttgctcct ttgaccaaat tcgtcgtaac gatcattgga
     9001 agcatttcag gtgttagacc aactgctaca gatagtccaa aaagaaaagc ttctagccaa
     9061 tccccttttg taagcccatt gatgacgata actgctggag ccatcaatga cataaatttg
     9121 atcagtagca tggatgtttt atggatgcct aattcaaagc ttgattgaat tggtttttca
     9181 gatagcgttt ctgctacatg accaaatagt gtttcatttc caacagaaac aatgattcct
     9241 tccgcacttc cactgatgac attacttccc ataaagacaa gattttcgta acttgtttcg
     9301 ctttgctgat cgtcttcaca tactttcgca tgcttttcta ctggatagct ttcccctgtc
     9361 aatgctgctt gggaaatgaa taaatccttt gcggacaata aacgcatatc cgctggtatc
     9421 atatcccctg ctgaaagccg aacaagatct ccacagacga tttcttccat aggaatctct
     9481 gtgtactttc cattacgttt tacagctgcc gttactttca caagtgattt cagtttttca
     9541 gccgcttgat tggaacgtac ggactgaatc aacgtcattg tcccgctgac gataaccata
     9601 gtgaagataa tggctacacc gaaaagatct ttatctccag gagctgctaa tacatattcc
     9661 gtgatgaatg aaataattcc taatgcgatc aacactaatg taaatggcgt gatgtatgct
     9721 ttcaatattt ctacaatgaa tggtgttttt ttgccatatg aaatcgcatt ttcaccgtaa
     9781 ttttctcttg aacgttcgat ttgttcttct gtcaatccat ttgctgatgc atggaatagt
     9841 tcaaataatt cttgtttcgt ttttccagca aaagctgcat agattttttg tgtatctttc
     9901 tttttttcca tctcaatctc tcctttgtgt ccaaaagaca gctgttctcg ctgcttttgg
     9961 tctttagcta ttcatcggat cgatagagaa aaaactgaaa ggggctgaga acagatagat
    10021 aatagccctg gcctttcctc tagatccttg tttttcgaat gactgtcatc catttgttct
    10081 catctccttt actaagtaat tttaattcat gacttccaat catttttcga atagttattt
    10141 ttccaattag accacctcct ttttattttt caccgatggg gatttgcggc aacaaaaaaa
    10201 cctctcgcca aatactcagc gaaaggtttg aaaaagcata ttaaaaaaag cttctcagat
    10261 ctcatcgttg agttttgaca ctgtacaacg taaaaagcag atacctgctt ttagctacct
    10321 tatgaaaagc cttatctcga caattcctgt tctacccatt ggcgtctctc gacgtttttg
    10381 ggcagtagcc tatgtttgta taggagcctc acctaacaaa tcttattcgg ttttccttcg
    10441 ttaatataac aactaaaaaa gaggatgtca aatcaatttt ccatgttttt catacttttt
    10501 ttaaaataca cacgttatat tcctgtctca cgcggtttta tttgtctcat tttccacctc
    10561 ccgctataat agaaatatag taggaaacga ggtgctgtta tggaaagaac acttgttatt
    10621 atcaagccag acggcgtgag acgccattta gtcggaagca tcatccaacg ttttgaagca
    10681 aagggactgg ccattgctga aatgaagttt gaaactatga ctcctgaatt agcaaaagaa
    10741 cattatcagc acttgactga gcgcagtttt tttgatgaac tgatcgatta catgacttcc
    10801 ggtcctgtcg tttatcttgt gctggtcggc gaggaagtga tcgatatcgt ccgtaagatg
    10861 gttggagcaa caaaagctgc cgatgctgtt ccaggtacga tccgaggaga ctacgcgctg
    10921 ccggggactg aaaacatcat tcacgcttct gattcccgtg atgctgcggt aaaagagatc
    10981 gcccgctttt tcccgccatt cgatataaac aaaccgtctg aaaaagcggc gaagtgatac
    11041 aaattctaaa ctgctggtct agttgctggg ttcagcaatg gccagcagtt ttttctctaa
    11101 ggacctccgt tgattctttc ctaacatata ttcgagcact gtgaagaatt cttggtagtc
    11161 tttcgtcttt tcttccccta aagcagcaga aatcaatctt tgataataat ctgccatcaa
    11221 tgtttcatag atcactgtac ttctttcttt atgtgctaag ctccgtttca tatcttcatt
    11281 cacactacgt gcctttttca accgaccatt ttctatataa aacgaagtca ggttcattaa
    11341 aaaagtaaag agatactgct catgattgtt caactgtgct aatcgtcgca ttcttttttt
    11401 gattttcaaa taattgttct caatgaaccg atcactgaat atccaaggaa aatcagctag
    11461 aatagatagt tcaaattgac catagtctgg tatctgcatc aagtattctt caatgattcg
    11521 gcattcataa cgtaaaaatt caaattctgt ctcagatact tctgtttttt cgaccatgtg
    11581 aagattcaaa accaaataat acagatcttc tgttttctta aaatgcctac ggttattgct
    11641 gatctcttta taaggatcaa agctgtcttc ttgtacccga tctagcattc gtcttttttt
    11701 ctttaacttt attggttcat ccagctggtt tcgcatccat tcatattctg caagtgtcat
    11761 gttcattcgc tctaaaaatg atctcaatgt tacaaatgac atttttttca ctgaatttgt
    11821 ctcaatcccg actagagaag tcttaaaaca gattccttcc gctaattggg actgtgtcaa
    11881 cttcttgttg actcgaagtt tcttgatcaa ttcaccatca tacataatag ttctcctttt
    11941 taatttaaat ttttccacat tcaataaata gatacgttaa aaaggaaaaa tacacgaaaa
    12001 aaataaaaaa atgttttttt cataaaaaat accaaccttc aagcattaga atgttgtcta
    12061 actactgaag gttggtttga ttgtttattg attatttttt tgctgttcaa cgtctgttct
    12121 tgttactgga tataagttgg ttgttgtttt attaccattt tttgaaaaga cacttgtcga
    12181 atgttcgcct ttttgctttt cgattgcttc attacgttcc gcttcatttt tatagtcata
    12241 ttttttcgga tcaatcgtct caaatccgtt tggcacataa aaccgcaaga gattttcctg
    12301 attcagctta tctgaaagac ttaacgcagt cttgactttt tcctgatctt tggagatttc
    12361 ctcatcgatt cctgctgctt tcgtatcgat gatttctcct gtctgattat tgtaagcttt
    12421 tcctcctagg acggtgtatt tgggtgtcac aaagttccca tttcgaaaag caactacttg
    12481 atcatgctga gaagataata agtctgtacc aaaatgaaga taatctttat catcgattcc
    12541 caatagatgt agtaatgtcg gcaaaacatc gatttctcca ccgtattcat gattgactgt
    12601 ccctttagta taaccaggca tatggatcat caatggcact cgttgcatct gtgcgttgtc
    12661 aaattcatcc catgtatctg gatctttacc aagagcgctt gctaaatctt tattatctgt
    12721 attcgagatc ccaaaatgat ccccgtaaat cacaaagatt gaattttggt aaataccaga
    12781 ttttttcaaa tacgtaaaaa attgttccaa tgattgatcc agataatgtg ccgttcgaac
    12841 ataattatct actgtgctat tgccagtatt caaagatgga aagtcgaaat tcttgtcatc
    12901 cgtataataa ggcgtgtggt tggttacgga aagaaatttc gtgtaaaacg gctgttgtag
    12961 atgctcaaga tattgggcag attctctgaa taaatcttta tctaatatcc cataacctaa
    13021 cgtttcgtcg gattcatcaa aataagatcg atcaaagaaa ttgtcataac ctaagttttt
    13081 atatacatga tctctattcc aaaaactagc tacattccca tggaaaacag cgcttgtata
    13141 gccttgcttt tgtccaagga tcgctggtgc ggcttgaaag acattgtctg acccaagttc
    13201 tgtgaataat gagccttgag gtaaaccaaa tgtgccagtt tccaacatat tttcagcatc
    13261 acttgtcttt ccctgtccta cttcatgaaa gaaattgtca aagctgatcg ttgcttgatt
    13321 ctggaaaata ctatttaaaa acggtgtgac ttcttgtccg tctaccttca tattaatcaa
    13381 aaactgctgg aaactttcta aatggagaac gatcacattt ttccctttag cgatcccaaa
    13441 tgtttcggga tttggctctg cataatgttt ctttgtataa tctaaaactt catcaatccc
    13501 attactgctt gcatgcgcgc gaacactgct agtcattcct gttttgattc cgtcataaat
    13561 agtatacgca tcgattccta gatatttgac aatatatgaa cggtcaaatg ttcgctgcag
    13621 caattgtgga cggttggctt cgctcagcgc taaatttcct gaaaaaataa gccctgaaag
    13681 acaggtaaca gcgatagcca tcggtttgcc gacagggttg cttttgatag gtattttctt
    13741 gaccttcaac caaaccagta gcccaatgaa cacagcgata tccagccagt agatgacatc
    13801 atggggcttc atcaaagaaa aggagcttcc tgacaatcct tgcgacactt tcgaaaagcc
    13861 taatacagat ttgactgtaa tgaaatctgt gaattcccga taaaaaataa tattcaagta
    13921 caacaaaagc gtatttaaaa tatcaatgat caaaagaata gctaacgctg gctttggctt
    13981 tttaatatac aaagaaaggc caaaaaacaa taaggtagtc gcaattgggt tgatccacaa
    14041 gataaagtac tggatcgtac cttccacacc taatgaaaaa tctgcatagt aggcaatgat
    14101 cgtcttcagc cagaaaaaga aaacagctgt caaaacgatt gctaaacgat tttggcacaa
    14161 ttttttaagt tgtgtcacgt atatcacttc tctctattca cattttttct tattttgctc
    14221 ctgaaaaatg aaaatttcct aaaaaagttg ttatcttaag atttttttat tctttctaaa
    14281 gattattccg tccacttttc acagcataaa accgctgcga tgtaagcgat taaactaatg
    14341 aagagataaa agtcttgtgg tgaccgcttc actccacggt attcaaaagc cagtttctcg
    14401 gtattcgtac aaaaaaagag gctgtgacaa aattcttgtc gcagctcctt atttcttgat
    14461 gcaggacagc tttttcacaa cttcttcctt aaacggtgtt ctcctgccaa ctcctcggcc
    14521 acaagtcaca atatttataa aacatgagaa gctgtttttt gaaattgttc cttgtggctc
    14581 agagttaaac ggcaggctca agacctcttg atacacggtg ttcaatcagc tgaccctcgg
    14641 tattagatta tgtcaggacg ctaattttac aacctcttgg taaacggtgt tcaaaagctt
    14701 gcatctgcgg taataagccc aaccaaacga aaaatataag gagctatttt ttgaaattgc
    14761 tccttgtggc tcagagttaa acggcaggct caagacctct tgata
//
LOCUS       DACEXQ010000041        14763 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.041, whole genome shotgun sequence.
ACCESSION   DACEXQ010000041 DACEXQ010000000
VERSION     DACEXQ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14763
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.041"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(66..431)
                     /gene="ssrA"
                     /locus_tag="IXH04_000967"
     tmRNA           complement(66..431)
                     /gene="ssrA"
                     /locus_tag="IXH04_000967"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(602..1375)
                     /locus_tag="IXH04_000968"
     CDS             complement(602..1375)
                     /locus_tag="IXH04_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1851595.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1519..1869
                     /locus_tag="IXH04_000969"
     CDS             1519..1869
                     /locus_tag="IXH04_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAQ1851596.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            1850..2566
                     /locus_tag="IXH04_000970"
     CDS             1850..2566
                     /locus_tag="IXH04_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1851597.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2566..4014
                     /locus_tag="IXH04_000971"
     CDS             2566..4014
                     /locus_tag="IXH04_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1851598.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4084..4593)
                     /locus_tag="IXH04_000972"
     CDS             complement(4084..4593)
                     /locus_tag="IXH04_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851599.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4583..5308)
                     /locus_tag="IXH04_000973"
     CDS             complement(4583..5308)
                     /locus_tag="IXH04_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851600.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(5531..7651)
                     /locus_tag="IXH04_000974"
     CDS             complement(5531..7651)
                     /locus_tag="IXH04_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ1851601.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            7881..9059
                     /locus_tag="IXH04_000975"
     CDS             7881..9059
                     /locus_tag="IXH04_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAQ1851602.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            9075..9833
                     /gene="aroD"
                     /locus_tag="IXH04_000976"
     CDS             9075..9833
                     /gene="aroD"
                     /locus_tag="IXH04_000976"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAQ1851603.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            9856..10341
                     /gene="ybaK"
                     /locus_tag="IXH04_000977"
     CDS             9856..10341
                     /gene="ybaK"
                     /locus_tag="IXH04_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAQ1851604.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(10412..11665)
                     /locus_tag="IXH04_000978"
     CDS             complement(10412..11665)
                     /locus_tag="IXH04_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1851605.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            11782..13131
                     /gene="gdhA"
                     /locus_tag="IXH04_000979"
     CDS             11782..13131
                     /gene="gdhA"
                     /locus_tag="IXH04_000979"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAQ1851606.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            13243..14589
                     /locus_tag="IXH04_000980"
     CDS             13243..14589
                     /locus_tag="IXH04_000980"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAQ1851607.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
BASE COUNT         4858 a         2629 c         3023 g         4253 t
ORIGIN      
        1 taaaaaatgc tcaaaccctt ataaaataag gatttaagca tctagcgaaa tctgctgaaa
       61 tctcacaatg gagacggcgg gagtcgaacc cgcgtccaaa cacatcgcca cttaaaaatc
      121 tacgttcata gtcaactcat ttaaggattc actttacagc ttgccgagtg acaggcattc
      181 tgtatcgcta gtctgatgtt ctcttttcaa cttacagacg gaaagttgaa acgtatccca
      241 ctaaagttag gacccttact cgagcacatg ggcgatgccg agaggatcta cgctaactgt
      301 ttttaggcag ctaaagcgta agagttgttt tcgtttttag cagttatatt taactgtaac
      361 gtttttacgt agacgtaacc tacgaaacgc aattcaagct cgactgtgcc tgtcgaatcc
      421 gtaacgtccc cgttagctaa caagggttac gtgtattttt catacaaaat tagtatagca
      481 tatattggga taaattcaat aaaaaagcga atcatatccc attatttata taataactgc
      541 ccctttctta agaaaaaata agcgattttc ttaagaaaag aggtagcttc aaaaaaagat
      601 cttaaatcag atcaaaatac ttcaatgcgt ggacaattcc gccttctgta tttttctttg
      661 tgataaaggt agaaagttct tttaatccgt cataggcgtt gcccatagcg attttgttgt
      721 cgcaagcttc caacaaggca aaatcattcg gaccatcgcc aaatccaaaa gtaggaactt
      781 tttccagctg aagttctttt tgcagacgtt tgacacctgc cccttttgag attcctttct
      841 tcactgtatc gatcgagtaa ggaccattcc gataaaaagt tagctcagga aaatgttcgt
      901 gatagtattc gtcacctgat tgagataaga tcagcaacat atttactgtg ttttctttaa
      961 agcctaaagg atcaatttcc ggaacatctg aatgaatgaa gtcgtaagca ttgatcattg
     1021 tttggctatg accggtacac cagattttct gatcgttata gtaagaaagt tcatgaccgt
     1081 tcaactgtac atgatcatac attttttgac attcttctga agaaaatgtt tgagaataga
     1141 tttcttcgcc gtctactcga atgaaagaac cattcatggt gaccgcagaa gtaatgcccg
     1201 catcttccat gatagagtgg atttctgctt ctgtacgtcc tgtagcgatg acggggagaa
     1261 cattgttttc ttttaatgcg ttcatagctt ttgccacttc gggtgtgatt tttgatttcg
     1321 catctagtaa tgttccatca agatcaaaga aagtgattgc acgataattt gacacaaata
     1381 aaactccttt tttattggaa taagtttatc ttaacaaaga atgaacaaaa gacaaacctt
     1441 tcgtgaagaa ttcctgaaca tattggttca gtcaacaatt tagagtatac tataagaaga
     1501 gaatttagga gggaaagaat gatttttttc agtattggta tcttattctt aataattggc
     1561 agtctttttc ggattctgcc ctcaaaagga aatctgcctt tttatgggta tcattcgcct
     1621 cttgccgcaa aaacagacgc tcattggcgg ctggctcaaa agacaagcgg aaactggttt
     1681 tttcttatgg gcttactgat ggcattgatc ggctattatt taaagacatc tgggcatacg
     1741 aattattttc tgattgaaat gcttctgcta gtatttccaa ttatgcctat ttttattatg
     1801 acagaaaaga aattacagaa atatgacctg gaaactggag ggaacgataa tgaatatttt
     1861 aatgattgaa gataatcaat ctgtttctga aatgatgcag atgtttttct taaatgaagg
     1921 ttgggaagca actttcaagt atgatggaaa agaagggctc gatgcctttt tagaaaaccc
     1981 acaacattgg gatatgatta cactagacct gaatttgccc acactggacg gtatgctagt
     2041 aggaagagag atccgcaaag tttcaagtac tgtgccgatt atcatgctga cagctagaga
     2101 ttctgaaagc gatcaggtga tcggcttaga gatgggggca gatgattatg tcacaaaacc
     2161 tttcagtcct ctgacgctta ttgctcgaat gaaagctttg catcgccgta gtgagttggc
     2221 agaagcaaga gaagaagcag tagtttcaga tgatcatttt gatatcgaga ctagtcattt
     2281 caagatgaat acgaaaacac gagaagcgta tctggacaac cggccaattg aaggactgac
     2341 gcctaaagag ttcgatctat tgtttacttt agcaaaaaaa ccacgtcaag tattctctcg
     2401 ggagcagttg cttgaattag tctgggatta tcagtacttt ggtgatgaac ggacagtgga
     2461 cgcccatatc aagaaattac gacaaaaaat cgaaaaggtc ggaccacaag tcatccaaac
     2521 tgtttgggga gtcggctaca aattcgatga ttctggtgtt gcctaatgcg ttatttgtat
     2581 cagcaactct tagctttctg cggaatggtc gcgttgatct tattgatcgt tggagtttct
     2641 tttacacaac tgacaaaaca aacgattgaa gaaaacaatt accagcaact gtttggatat
     2701 gctgaatcag ttgaaaagac aacgcaaacc tatgccgata cgctcccgca gtttagtcaa
     2761 gatcaggcgt ttcaaaatgc attgctgctg acagagcaag tactgacaga acaaaatgtg
     2821 aactttatat ttatcgataa aaacgaacgt gtcatttatc cgactaatac agctttactg
     2881 aattttacct taacagctga tcaatggaaa aatctgagga atggacgtca agaaaaattc
     2941 acatcgaata aaaatatttt aggacaagca caggcgacct cttacgcact tgtgccattt
     3001 aatttgaatc atgaattcta tggagcactt gtagttagtc aacctgcgcg aaatatcgat
     3061 aacagcgttc gtccaattac actgaacctg ttcaaaggat ttattttttc cagtatcgtt
     3121 gcagttattg ccagctatgc ttttgcagca ttccaagtta agcgtatcaa tcggttaagg
     3181 aatgcgacga aagaagtgac gaatggtaat tttgatgtac agctccccgt acatgacaaa
     3241 gatgaatttg atgagttagc agatgatttc aataaaatga cgaattcatt gaaagaatct
     3301 caagcagaaa ttgaagaaca agaaaatcgt cgaagacaat ttatggcaga tgcttctcac
     3361 gagatgagaa cacctcttac gacaatcaac ggacttctgg aagggttaga atacaatgca
     3421 atccctgaaa atcagcgaga aaacgcgatt aaattgatga aaaatgaaac agagcgtctg
     3481 atccgtttgg tgaatgaaaa cttggattat gaaaaaatcc gtacgaatca aatcagtatg
     3541 gtcattaaaa aattcaatgg aacagaaaca ttgaaaaata ttgttgcaca gttagaagct
     3601 aaagcagaag cagcaggaga tacattgaca ttgaaagccg ttgatgatat tgatgtttac
     3661 gctgattatg accgatttgt ccaaatcatg gtcaatatta ttcaaaacgc gatacagttt
     3721 actgaaaacg gccaaatcac gattacttta gaaaaaggct atcttgaaac gatcatcacg
     3781 attgaagata cgggaattgg aatgtctgaa caacaaatga aaagtatctg ggatcgttat
     3841 tataaagtag atccttcaag aaaaaataca aaatatggtg aatcaggatt aggtttacca
     3901 atcgtgcagc aacttgtacg cttgcataaa ggaaaattag aagtagaaag cgaactagga
     3961 aaaggaacaa aattcgctgt tcgtttgcct gacgtagaaa tcaaaaaaga gtaaaaaaca
     4021 agcggtataa aagatcatga atcgatcttc tataccgctt gtttttacta taaaatgatt
     4081 gatttagccc gcgggctcta aacgatcaat ataaggactt tgagcaatag tagcatcatg
     4141 agtaaagagt aagatacctt gattttcttc atgagctaaa taagaaagca agtggagtag
     4201 ctcttggatc tccaatacac taagttcatc aaatgtatct tcaataacaa tattgtttcg
     4261 cttggaaagc aaggcatgaa gtaattgcag ctttactttt tcaaaagacg tcaactcagc
     4321 tgccgcacaa ttaagcaagg aaggagagat atcaagtttt tcttgatagg ccaaacgatc
     4381 ttgtttgatt tctttttccg gtacatccaa caggacatgg tctttaagat tcagataagg
     4441 aaacatcggc caattttttt ggatgatcgc aaaattatcc agttttgcaa caagaacctc
     4501 ttttttcaag tgctccaata ttttatgctg tttttcgaga ggacacaaaa taacggtcaa
     4561 gtgttcagca tgaatcaaat attcaggttt cattacgtcc aggcctccta ctttgcaaat
     4621 gatggataaa aaaagtgaaa gggaatagac taagaatgag acagcatcca ctgagcccaa
     4681 taagagtttg ggcaaatccc ttaagtatat ctgtgaaaaa aagatcattt tggaaatcga
     4741 tggtaaaaaa agtctgttga tccatcggaa ttgtaagtcc gcttaccgaa gtcaagatat
     4801 cacggatatc tggcagttca gtagtcttta agacaagaaa attgatcctt tgaagaagcg
     4861 cttcataaac tgactggaag cttacgatcc acaataaaac caagatacaa caaagaaaga
     4921 gaggaaagaa cagctgccag aaaataaaaa ggaataattt ggttttagaa agaccagtca
     4981 ggtgccaatt gatcaattcc tgcttgtttt gcctgactga atagtaaaag aaaaagataa
     5041 aaaggaaggc aaatagaaaa aataaactga atatgccttt tatataaaat cggctagcta
     5101 attctaaagc cggaagaagc tctgtagatg cagaagcgcc aataaatgaa agccgatctt
     5161 ggacttgatt ggctaaagcc gattgcaaat ggtaaagagt caggcatgct atcaataaaa
     5221 aagcagaaaa gcttgaaaat aaacaataga gtattgtctg ctttttgtaa taaattaatc
     5281 gagcagtagc atagcggatt attttcatac ttttcacctc tataaaaagt atagaaaatg
     5341 aatatgaata aagtatgaaa ttcctttagt gaaatcacga attaaaacca gaaaatattt
     5401 tgtaaaaagc aagaggttgt gaagaagctg ccctattcta ttaccgtaga tgcaagcttt
     5461 tgaacactgt ttattcgaaa agaggagcag cgacaataat tttgtcgctg ctccttttaa
     5521 atttatttta tcattcggaa gaacttgtgg tcgttccgtg aagttcttgg tacgtttgcg
     5581 ttttataaag atcaacagtt gattgtccgt tgtgttcact aaagacacta gttgacttgt
     5641 caccaagaga tttttcagct tcttttaatt tctctaagcc attcttgtag tcataatcac
     5701 tggtatcaat tgggtctaat ccactattcg tatagaaccg aagaagatca ccattattga
     5761 tttggtcaga aacagctaac tgtttatcaa ctttttcttt cagtgaatca atttcattct
     5821 gtacttcctc agtaggttca gtaatagctt caccagtttc gttactatag atcgtaccac
     5881 cgtagtaagt atattttggt gtaacaaaat caccatcacg gaaagaaaca agctgttcat
     5941 gatctttcga gaatagatct tgtcctaact gaatataatt ttttgtatct acacctagta
     6001 agtgaagtaa tgtaggcaag gcatcaactt gtccgccgta agtgtgattg attccgccct
     6061 gtgtttgtcc agggatatgg atcatataag ggacacgctg catttgtgca ttgtcgtagt
     6121 ttgtccaagt agaacttgtt tttccaacta attcagctag atttttattt cttgaattcg
     6181 agacaccgta atggtcaccg taaaggacga tgactgaatt atcatacaag ccgctttctt
     6241 tcaaataatt gaagaattct tgtacggcgg tatctaaata attggctgtc gcaaagtaac
     6301 cgttgatcgt ttcatcattt gtattggcta acgggaatcc tgcttcgtca ttggtgaatt
     6361 ttgaataagg ataatggtta gatacagcaa tgaattttga ataaaatggt tgttgcagac
     6421 gttccaagta ttgtacggat tgttggaaaa atggtttatc atgcaacccg tattggaacg
     6481 aattgtcatc attaacatca taataagaag aatcaaagaa ataatcatat cctaaattct
     6541 tgtatgtctc gtttcgattc cagaatgtac cagcgttccc atggaatacc gcactaatgt
     6601 aaccttgtgt ttgacttaaa atatcaggtg cagcttggaa agtatttttt ccgcctaatt
     6661 gtgtgaacaa ggagccttga ttcaagccaa acaatgaatt ttccatcagc gtttccgcat
     6721 cgcttgtttt tcctgctttt acttgatgga aaaagttgtc aaaactgaac gtgctgttgc
     6781 tgtggtaaag actattgatg aaaggtgtaa cttcatgctg gagaccattc tcatctgtca
     6841 atttataatc gatcaggaat tgctggaagc tttctaaatg gatataaatc acattttttc
     6901 ctctagcgag cccgaacaaa tcgtcatttg gtgctgcata atgttctttt acataatttt
     6961 ctacttcttt catatcattg ggacttgctt ctgcccgcac ttggtttgtc tggtaagttt
     7021 gaacaccgtc ataaaccatg aaagcattca agccaagata tttgaccaaa taatcacgag
     7081 agaatgttcg agtcagcaat tctggacgat cagcttccgc cataaaaaga tttcctgaga
     7141 aaatcataac tgctaatgta gagatcgcaa aagccattct tgctcggatc gggcgagggt
     7201 ccaacttgat tttttttgca atcagcagaa cagtcaagat aataaaatcg atccaataga
     7261 tcacatcata tgggcgaaac agtcgaatgg cactttctcc cagaccactc gctactttcc
     7321 cagcacctaa aatcgtgttg atcgtaatga agtccgtgaa ttctcgataa taagtgacat
     7381 tcgaaaaaac aagcaatgtc aaaagaaaat aaatgaccat catcgtgata taagcaactc
     7441 tagggcgtct gatataaaga gcaattccaa gtaataacaa tgtagttgca atagggttga
     7501 taaacaagat aaagtattga atgccacttt cgattccgag atggaaatca actaaatagg
     7561 caaaaatcgt tttcaaccaa agtaaaacga ctagtaaaga aaaaaatccg agcctcttat
     7621 tcaaaaaggc aggtgttttt atacatttca agataatctt cctttctgca cttcttaatc
     7681 acagaagtgt cctgaaacca tgttacacag ctcacttatt ctcatttatt ttactagtat
     7741 gacaatatag tgtcaattga aaccagaagt ttatgctgtt tcattaaggt tttctttaag
     7801 cttcatgaaa aaatgagcag taagacaagt cctatgtttt ttgttatact tgggaagaga
     7861 aagctaaagc aggtgaagga atgaaagtaa aagtaaaaaa aagagcaagc aatcgattga
     7921 aaaaaggata tccactgatc caaaaagaag accttgctca tccccaaaaa gaaaccacgc
     7981 aatgggtgga attagttgat tcgcaaaacc aatttatcgc aaaaggatat ctaggaaagc
     8041 agaataaggg aatcggttgg gtattgagcc agcagaacat cccatttgat caatcttttt
     8101 ttgaagaatt ttttgtgaag tcaaaagaaa aaagaacttt ctttttttca gatgattcta
     8161 ctaccgcttt tcgtatattc aatggagaag gagacgggat cggcggtctg attgttgatt
     8221 attatgcagg ttatgctgtt ttttcttggt ataacgaaac gttatatgca catcgtgaat
     8281 tattgattca agcttttcgt gaaatctacc cggaaatcat aggaggctat gagaaaattc
     8341 gatttgaatc agtaaatctg cctgagagcc agttcattta cggaaaagag gcaccggaac
     8401 ccttgatcgt aaaagaaaac ggagtaaaat ttgccgttta tttaaatgaa ggattaatga
     8461 ctgggatctt tttagaccaa aaagaagtac ggggatcatt agcagaaggt ttcgcttctg
     8521 ggaaaacagt tttgaacatg tttagttata ccggcgcctt ttctgttgct gccgcaatgg
     8581 gaggggcaat cgaaacaaca agtgtcgatc tagcaaaacg cagtttaaaa aaaacacagg
     8641 aacagtttga agtgaatggt ttggatgtcg atcagcaaaa aatcattgtt atggacgtat
     8701 tcgagtattt caaatacgcc aaaagaaaac aatttttata tgacatgatt gtattggatc
     8761 caccaagttt tgccagaaat aagaaaaaag tctttcgcgt agcaaaagat tatggggaac
     8821 tggtcaagga tagtctggat attttagcag aagacgggat acttattgcg tctacaaatg
     8881 ctgcgaatgt ctcgatagaa caattccaaa caatgattga agaggaattc catgccaaaa
     8941 atgtttcttt tgaacaactt gctttatatc gtctgccaaa agatttccag acgacggata
     9001 cctttttaga aggcaattat ctgaaagtgt ttgtttatca aattaaaaat aaccattaga
     9061 aggagcgatg cagaatggtt cgggtaaagg gattgaatat aggaagcggt agaccaaaga
     9121 tttgtgcgcc tcttatcggg gccgtccgag aggaagtatt acgtgaagca gttcttgcac
     9181 gtgaagcagg ggccgattta gtcgaatggc gagtcgatca ttatcgtgaa gttttttctt
     9241 ttgatgaagt agcagaaaca ttggttctta tccatgagat gcttgatgga ctgccgttat
     9301 tatttacgtt tcgtacctta gctgaaggcg gagaacaaga catatcgatc cgtcagtatc
     9361 gcgaccttta tgaacatgtg atacacacag gattagtaga tatggtcgat atcgagttgt
     9421 tcaaagtaga aagtctagga aaaagactga tagaggaaat caagtcttta aacattcctt
     9481 taattatcag tagtcatgat tttaaagaaa caccagcaga ccctgtcttg ctttaccgat
     9541 tgaatgttat ggaacatttt ggagccgata tcggaaaact ggcagtaact cccaataatg
     9601 aacgagatgt tttgcggttg atggagttga ctagacgggc aaatgctttt gtctccatgc
     9661 ctattattac gatgtccatg ggaaatcttg gtaaaatttc tcgtctggtt ggcggaacga
     9721 cgggttccgt tatgacattt ggttccttaa gcagcgattc tacttctgct ccaggacaat
     9781 taccggtcaa agcattgaag caaattattc aattattaaa tgaaccgttg tagaacaagt
     9841 ttgaggtgat attttttggc aaagaaaaaa atagtaaaaa cgaatgcaat ccgtatggtc
     9901 gaacaaaaaa agattcccta tacagagcac gaatatgagt gggacgaaag ccacctaagt
     9961 gcgtcatcgg tcgctgaaca gcttccagaa agccaatctc gtatatttaa aacactggtt
    10021 gctgtgggca atgtgacggg gcctcttgtt gctgtgatac cgggagaagc cgaattgaat
    10081 ctaaaaaaat tagcaaaagt ttcagggaat aaaaaagtag aaatgctcca tttaaaagat
    10141 ttggaagcaa cgaccggata catacgtggt ggctgctcgc ctataggtat gaaaaaacta
    10201 tttcctactt atttagatca aattgcagaa agctatgaac aaatcattgt ctcggctggc
    10261 agaagagggc tacagatgga gttagcacct caagatatct gtgcattaac gagcggacaa
    10321 tttgcagata taaaacaata aatcatacgt ataaaagaaa gaaggaggcc gggatgttgt
    10381 ttgtccagtc tccttctttc ttttttatac attatgctct ttgcccggca ttcattcctg
    10441 ctacatgccc tgtagcgaaa gccgctgtga tattaaagcc gccagtataa ccattgacat
    10501 ccagtaattc tcccgcaaaa tacagaccat caatgatttt actctccaat gttttaggat
    10561 aaatttcctt tagagaaacc cctccgccag ttacaaatga tttctcaata ggaaaagttt
    10621 tgttgattga tagcttaaac tctttgcata gttgaacaaa atcttgaatt tgtttttgag
    10681 aggcttgttg tcctgtcaga tgatccattt caagccgctc aagaaagaaa actaacaatc
    10741 gctcaggcaa tataccatgc caagcattga ccaagttctt tttggttgct ttactttttt
    10801 ctgtcaatat atgaataagc tcttgtttgg tttgtgtagg gaaacaatcc agagaaagtg
    10861 tcactggttc tcccgttttc tctaattctt tattgacaaa acttgaacaa cgcagtgcag
    10921 ccggaccaga aataccgaaa tgggtgaaaa gtaaatccat cgtatgttca gtcaatacac
    10981 gacctttttt atttaaaacg cgtaaagtga tgtcttgtaa agaaagccct tgaagtgttt
    11041 tctcctgaat atacggttca tccgatatca agggggactc tgtcgcataa agaggcgtca
    11101 ccgtgtgacc cactcgcttc acgatcttgt acccatctcc agtagaacca gtagatggat
    11161 aagtccgtcc accagttgtt aagataatgg caggagcatg gaactcttcg aattccgtgc
    11221 gtataccgta aattttatgt tctttgtgga tcaatttctc tactcgagta gagaatagaa
    11281 gcgtcacatc tagttctttc agacgattga gcaatgcttc gataatcgtt tttgatttat
    11341 ttgttacagg aaacatccgt ccatgatcct cttcttttaa atgtacgcct tgtgattcaa
    11401 agaagttcat gatatcgaaa ttattccatt gcgcaaacgt actatataaa aactttccat
    11461 ttccaggaat atgtgcaatc aaatcatcaa caggacgatt gttcgtaaca ttgcaccgac
    11521 caccacctgt catcagtagt ttttttcctg cttttttgtt tttttcgatc aagagtacgc
    11581 gtgcgccttg ttcagctgca ctgatcgctg ccatcatccc gcttgttcca gcgccgacta
    11641 cgatcacatc atacagtttt tccattccct cacctcaagg aaagtgtagc atatctgaag
    11701 aaagaaaaca gaaaaaaatg ttttgagcca aaagagaaaa tgagctaaaa tgagggaaga
    11761 taagcaaagg agcgtggaac gatgacaaac gcaacagaat atgttcaagc aattcaagaa
    11821 aagctacatc aaaaggatca agggcaagaa gaatttttac aagcaatcga tgaattcatg
    11881 cccacggtta tgtctttttt ggacactcat cccgaatata tcgaaaagaa tatcttagaa
    11941 cttttaacgg aaccagaaag agttattcaa tttcgagtgc cttggcaaga tgatgaagga
    12001 aactggcgag taaatagagg gtaccgtatc caatacaact cagctatcgg cccttataaa
    12061 ggtggattgc gcttccatcc tagtgtcaat ctaagtatct tgaaattttt ggcttttgaa
    12121 cagattttca agaatagttt aaccggtctg ccaatcggtg gaggaaaagg cggaagtgac
    12181 ttcgatccta aaggaaaatc tgataatgag atcatgcgtt tttgtcaaag tttcatgctg
    12241 gaattgtcaa aacatatcgg cccatcgatt gatgttcctg cgggagatat cggtgtagga
    12301 gctagagaaa tcggctattt gttcggcgaa tataaacgat tgaaaaaata tgatactggt
    12361 gtgttgacag gtaaaccttt ggatttttgg ggaagtaaga tacgtacgga agctactggt
    12421 tatggattag tgtattatgt caaacatcta ttgaacgaag aaaaagattc atttaatcaa
    12481 aaaacagttt ttgtttccgg aagcgggaat gtagccattt atgcaatcga aaaagtgcaa
    12541 gagctaggcg ggaaagttgt tacatgttca gattctagcg ggtacatcta tgatccagaa
    12601 ggaatagatg tgaaactatt gaaagaagtc aaagaaatca atagacaacg tttgacggaa
    12661 tacgcaaacc aacgtccttc tgctatttat catgctggag aatctgtttg gacattgaaa
    12721 gaaaaagcgg atatcgcttt gccatgtgct actcaaaatg aaatagacga aagcttggcc
    12781 gaaatcttag tagaaaatgg gatcaaaatc gtagcagaag gcgctaatat gccatgtaca
    12841 gtaaaagctg tttctgttct tcatgaagca ggcgtgtggt attgtccagg aaaagctgct
    12901 aatgctggag gagtagcagt atctgcttta gaaatgagtc agaacgtcca gcgattgact
    12961 tgggaaagag agcaagtgga tgagcagctg gatcaaatca tggaaacgat ttaccatacg
    13021 tgtcgccaaa cagcagccga gtacggcgac gaaaaaaatc tgcttcttgg agcaaatgtt
    13081 gctggatttg aaaaagttgc caaagcaatg gcagcagaag gtttagtttg aaaaatgaaa
    13141 ctttcttgga aatattgtca gcattttgtt tatttgcttg aaaaataata aaaaaaaacg
    13201 taaagtatta cctgtagagt aaacctataa ggaggagaac aaatggcaca cattcatttt
    13261 gactattcaa aagtagcacc gtttgtcaac gaacaagagt tagggtacat gcaaagccaa
    13321 gtaacagcag cccacaacga attacgggaa ggtactggcg caggaagcga cttccgcggc
    13381 tggatcgatt taccaacgaa ctatgacaaa gaagaatttg cacgtatcaa agaagcagct
    13441 aaaaaaatcc aatctgattc tgaagtatta gttgttatcg gtatcggtgg ttcttattta
    13501 ggcgctcgtg cagcgatcga cttcttgaat cacacattct tcaatttatt agatagcaac
    13561 acaagaaaag caccacaaat cttctttgcc ggaaattcaa tcagctcaac atatattgct
    13621 gacttgatcg aagtaatcgg tgatcgcgac ttttcagtaa acgttatttc aaaatctgga
    13681 acaactactg aaccagcgat tgcgttccgt gtattcaaag agcttcttgt taagaaatat
    13741 ggacaagaag aagcaaacaa acgtatctac gctacaacag ataaagcaaa aggtgcagtt
    13801 aaagtagaag cagatgcaga aggttgggaa actttcgtga ttcctgatga catcggcgga
    13861 cgtttctctg tattaactgc tgttggtttg ttaccaatcg ctgttagtgg tgcagatatc
    13921 gatgcattga tgcaaggtgc cgctgatgca agcaaagcat actcaagcga caaactagaa
    13981 gaaaacgaag cttaccaata cgcagcaatg cgcaacattc tttaccgtaa aggcaaagta
    14041 actgagttgt tgatcaacta tgaaccagga atgcaatact tctcagaatg gtggaaacaa
    14101 ttgtatggtg aatctgaagg aaaagaccaa aaaggcatct atccttcaag cgcaaacttc
    14161 tcaactgatt tgcactcttt aggacaattc atccaagaag gacgtcgcaa tatttttgaa
    14221 acagttgtga aggttgaaaa acctcgtaaa tcaatcacaa ttcctgaaca agcagaagat
    14281 ttagacggtc taggctactt gcaaggaaaa gaagtggact tcgtaaatac aaaagcattt
    14341 gaaggaacat tgcttgctca tacagacggt gacgtgccta acttgctagt caaaattcca
    14401 gaaatggatg cttatacatt gggttataca atgtacttct ttgaaattgc tgtaggtatt
    14461 tcaggttact tgaatggaat caacccattc gaccaaccag gtgtagaagc ttacaagaaa
    14521 aacatgttcg cacttctagg caaaccaggc tttgaagatc ttgctaaaga attgaacgaa
    14581 cgcctgtaag ataagggttt ttcgctagat ttaaataatc agtgatagaa acatgttcgc
    14641 tttaaaatga aaactggtac ccatttaggt actcaaacat actgcatcgt ttattcagaa
    14701 taaggggctg tgacaagacg tttgtcaggc tctttgtcaa atgatgttgg taaagagaaa
    14761 tct
//
LOCUS       DACEXQ010000042        14541 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.042, whole genome shotgun sequence.
ACCESSION   DACEXQ010000042 DACEXQ010000000
VERSION     DACEXQ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14541
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.042"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(76..420)
                     /locus_tag="IXH04_000981"
     CDS             complement(76..420)
                     /locus_tag="IXH04_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAQ1851608.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(574..1494)
                     /locus_tag="IXH04_000982"
     CDS             complement(574..1494)
                     /locus_tag="IXH04_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1851609.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            1681..2172
                     /locus_tag="IXH04_000983"
     CDS             1681..2172
                     /locus_tag="IXH04_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAQ1851610.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(2196..4571)
                     /locus_tag="IXH04_000984"
     CDS             complement(2196..4571)
                     /locus_tag="IXH04_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAQ1851611.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            4897..5523
                     /locus_tag="IXH04_000985"
     CDS             4897..5523
                     /locus_tag="IXH04_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ1851612.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            5546..6250
                     /locus_tag="IXH04_000986"
     CDS             5546..6250
                     /locus_tag="IXH04_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ1851613.1"
                     /translation="MTETTLYIVRHGKPMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            6442..7275
                     /locus_tag="IXH04_000987"
     CDS             6442..7275
                     /locus_tag="IXH04_000987"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAQ1851614.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            7472..9400
                     /locus_tag="IXH04_000988"
     CDS             7472..9400
                     /locus_tag="IXH04_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAQ1851615.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            9538..9993
                     /locus_tag="IXH04_000989"
     CDS             9538..9993
                     /locus_tag="IXH04_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAQ1851616.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            10463..10732
                     /locus_tag="IXH04_000990"
     CDS             10463..10732
                     /locus_tag="IXH04_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851617.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            11132..11386
                     /locus_tag="IXH04_000991"
     CDS             11132..11386
                     /locus_tag="IXH04_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAQ1851618.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(11475..12521)
                     /gene="holA"
                     /locus_tag="IXH04_000992"
     CDS             complement(11475..12521)
                     /gene="holA"
                     /locus_tag="IXH04_000992"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAQ1851619.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(12559..14532)
                     /locus_tag="IXH04_000993"
     CDS             complement(12559..14532)
                     /locus_tag="IXH04_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAQ1851620.1"
                     /translation="MEGRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFI
                     SPAKKRNLYNFDQEAYFRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSE
                     FPQKTSVYINSLLWGYKDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRI
                     GWPLHVSIIPLSLLTICYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVF
                     WLLLLFDPRFLFHAGGQLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFY
                     EWPILGSLFTLLIAPVFKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSS
                     GSSLIIGRLPLVLSGMYIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTI
                     AFIDVGQGDSIFLQSPFRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQG
                     ISVIDKLILTHDDTDHVGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEI
                     IEIKHGDRISGYYDLYVLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLL
                     LIYPNLKADVVKLGHHGSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEE
                     NQIKTWRTDQQGMITYRWHPVFHPHGLVETMID"
BASE COUNT         4478 a         2790 c         2590 g         4683 t
ORIGIN      
        1 ctcttttctt ctagaaagga taaaaggctt ttttgttttt cctatttgct tctcatagct
       61 agtcagccaa gatttctatc gtatccccaa cgcaatccgt gcataacggc tcatcttatc
      121 tgtcgtccat gctggatacc agactagttt gacttctgct ttcgttactt ctggcacttc
      181 ttttaatgcg tcatggatag attctgttaa aatatctgct aatggacagc ccatagttgt
      241 tagagtcatc ttaatgactg tttctccatt ttcaggattg aattctatat cataaatcaa
      301 gcctaaattg acgatgtcga ttcctaattc aggatcaatc accatttcca aggcagcaag
      361 aatacgttct ttgattagtt ctatttcttg tccagaccgt tcttggtttg cttcgctcat
      421 cgtacgacac tccttaacac gatttttatt tatcatactc tttttttagc tgcttgtaaa
      481 tgagaattta gaagtaagat tctcgtttaa atttataaaa atgttcttat tagatgtaat
      541 agattaaatt tattctaaaa taaacctgat tcctcatttt ttttgtgaac gattttttat
      601 ttctggcaat acgccagttg ttcgatacat tttttgttcg ttttcatttg caaaccggcc
      661 aaatactgaa tgagttggta aaatattgat aaatgcatcg tcgtcgattt catgaacgat
      721 ctgatcaaga tcatacattt cgtagcgggt gatgaccatc atgatcatgt tcccttgttt
      781 ttttgaatag ccaccgacag acggcagtaa tgtcattcca cgtaccatcc gttttgctag
      841 ttcttctgtt acctcttctg gcttgacggt aacgatcatt gctgttactt tttgatgact
      901 cgtgtggatg gcatcaatta cttgagtcat tgcataaatg gaaataatcg tatacaaggc
      961 actttcccag ttaaagaaga agcctgcgat gactacaatg ataccattta gaatcagcat
     1021 gtagtttccg actgtttttc cagttgtttg tgaaagtaca agagagatga tatccatccc
     1081 accagttgta aagcccattt ttaaagataa tcctacgcca agacctaata aaacaccgcc
     1141 tactagggca ttcatcaaaa tattatcagt gatctgtccg actggaagca gcatcgtcat
     1201 caccgataca catacaacat tcacaaaact aaggatcgtt gctcgttttc ctactttgag
     1261 aaaccctaat acaaacacag ggatattcaa aagaaaaata aatgttcctg tgtctatctt
     1321 gatatgaaac cattcatata aaagcgagga aatgatttga gcgatcccat tcatccctgc
     1381 agaaaagaca ttggctggaa tcaaaaaata atttaaagcg accgcagcgg tcaatccggt
     1441 tatcaaaata accactattt tctttaatct ttcattttta tgatacgcag tcataatttt
     1501 actcacaagc acctctcccc tactttggta ttccaaatgt aagaataaca gtttatctat
     1561 taaaataaaa tacttcgaac tcaaatgaga agaattttag caaacaggta agaatctgcc
     1621 ctaatcctat gataagatag atgtatggta aatatttata tgtatatcag gagggttcct
     1681 atggcaaaag atgctagctt cgacattgta tcagaagtca atacagaaga agtaaaaaat
     1741 gcgattcagc aaacaacaaa agaattgtcc aatcgctttg acttcaagga ttctacagta
     1801 gaaatcaaat tagagaacag caatctagtt gtattgagcg atgatgattt caaaatcgaa
     1861 cagatcaaag atgtattatt tggtaagctg atcaaaagaa atgtgccaat caaaaatatc
     1921 catttttccg aatcagaaca cgctctaggt ggcaaagctc gacaaactgc cgatttggtc
     1981 aacggaatcg atcgtgaaaa cgcgaagaaa atcactacag ctatcaaaaa tgaaaaactg
     2041 aaagtaaaag cacaaatcca agaagatcag attagagtga caggtaagaa ccgtgatgat
     2101 ctacaagctg tgattgcttt attacgtaaa cttgacttac caatcgagtt gcaattcact
     2161 aactatcgtt aatcaaaaaa cgaggctgac gtctgtcatt cctcgttttt ttgatttact
     2221 gcttcttttg ataaccaaaa acgctgtatt ccttcttcca attccccaac acttcgtact
     2281 tctcttgctg tttgccaatg ttgtgggaat aaacgtcgat aagaaaaact agaaaatcgt
     2341 tgatctgcca atacaacgat tccttgatcc gccatgccac gaatcactcg accaccagct
     2401 tgcaagactt tgttcattcc aggaagctga taagcgtaag caaatccaag gtgttccttc
     2461 tcatcataat atgatttgat cagttcctgc tcatgattca tctgcggcaa acctacacct
     2521 acgatcatac tgccgatcaa tcggtcccct ctcaaatcga ttccttctga aaaaatcccg
     2581 ccaagtacgc aaaatccaat caatgtttca gcgggatttg tttgaaattt tgttaaaaat
     2641 cgttcgcgtt cttcctcatt catgtccgtt tcttggatta gcacattgct gtcgggatat
     2701 tgctgtctga atgcttccac cgcaagatct aaatattgat aagaaggaaa gaaaacgaaa
     2761 taattcccga cctttccatc agcaaaagca tgaatcgtct ctactaattt tgccagactg
     2821 tgttcccttt ttcgataagt cgtctctagg taatttgcga tgaaaacttg ctggtttcct
     2881 tctgggaaag gactcggcag tttgtaagcc agactttttt gtccccccag tgtttcctga
     2941 taataagaga gtggtgaaaa actagcagaa aaaagaatac tgctttttcc tttatccaac
     3001 gactgctcaa gaaacaagct tggatcaata caaaactctt ttactatcag atcgcgatac
     3061 gttttttcga ctgttgtttc gtagtgatca tcataaaatt cactgacctt caaaaaatga
     3121 agcaaatcaa aataataagg aagcaattgt tcttgtgatg ggtgctcagg aaattctgcc
     3181 agccattctt taattttttc gcttaattga taacctgcct ttactaaaga ttcagccggt
     3241 gctttttgat gatgataatc ccaatgatct tcttttgcta tttctcggat cgaatcaaat
     3301 tcttttaata gtttgttgaa acggcgatga agttttttga attcctttgg tatagcttcc
     3361 ttacttcgtt tcgttttttc ataagatagt tctgccgaat acatctctcg ggaacgattc
     3421 accaaattat gtgcttcgtc aatcaaaaac agatagtcct cgtttttttc atcttcaaaa
     3481 aaacgtctta aataaacagt tggatcaaat aaataattgt aatccccaat gattacgtca
     3541 caccagatac tgacatctaa tgatagttcg aaaggacaaa gtgtatgctt tcttgcataa
     3601 gtttcgatga cttcacgagt gatctgattc tcatgattca aaagatccca taaaccttca
     3661 ttaatccgat tgtaataacc attggcatat gggcactgat ctggattaca ggtcgtctca
     3721 tctaaaaagc agatcttgtc tttcgccgtc aaagtgacac tttttacttc agctcctgta
     3781 tcttttaatg ctgtcatagc atcttcagct acttgtctag ttattgtttt agccgttaga
     3841 taaaagatct tttctccctc ttcttcccct acagctttca aagcggggaa taacgtagag
     3901 attgtttttc ctgttccggt aggtgcttct acaaacaatt tctgtttcgt acggatcgtt
     3961 ttataagcag ctaccgctag ttcacgctga cctttcctat actcatcatg ggggaatttc
     4021 aaagccatca aagaggcatt gcgtacagtg cgccagtttt cttgaaaaac aagccattct
     4081 ttataatctt cagtcagctt tttaaaaaaa gtttctaact gctctcttgt ctgatgttca
     4141 acctttctcg tgatcacttc ttctgccgtc tggaagtaag tcaattgcag atttatttct
     4201 tgtaaatcat gttcttgact atagatatag gcatacaccc ttgcttgatg aaagaaaagt
     4261 tccacttgtt ctggttctaa atcttcaaat cgtggttcgg aggttttgat ttcatctata
     4321 taataaatac cgtctctttt gaaaataccg tctgcacggc cttcaacgat caggtaatat
     4381 tcatctaatt gaactgttgt ttttaaaaag acttcttttt catagttttc tcccgcatct
     4441 ttttggagct tgcggtggat tctcgctcct tctaatgcag tatggttact ttttttacga
     4501 ttgtcgatac ttccacgtcg caaaataaat tctactaagt gccgcacaga tatttttgac
     4561 tgtttgatca tttccaccac ctctttctct ctaagattac ctgaaaaaac ctagtttagc
     4621 aaaaagagct ctctttccaa acataaaaaa acaccctgct gattaggcag ggatatagga
     4681 tatagaattt ggatttttgg atgaatctaa tgtaacaagg acatattaaa atgctatgaa
     4741 aacttacttt ccttctcttt agaatatcca aatacttttc atatagtctg ataattcaga
     4801 taagtaaaga gaaataagta cctgtttttg tttcctagca atgaatgcgt tatactaatg
     4861 gatgaaagaa gttttggcta tcaaggagga atgcacatgc atatcgaaag attaaaaaca
     4921 ggaatgattg aagaaaactg ttatttggtc tacaacgaag aagcactttt gatcattgac
     4981 cctggagaag atgcggaacg aatcaaacaa gaaatcaaaa aaacaaacca acagccggta
     5041 gcgattttgc ttacccatac tcactacgat catatcggtg cagtggaacc tttacgcgcg
     5101 tattatcaaa tcccagttta tgtcagccca ctagagcaaa aatggctagg tgatccgatt
     5161 ttgaatcttt ctggactcgg tagacatgat gatattgctg atgttattgt ctcgcctgca
     5221 gaatatgaat ttgaaatgaa aacttatact ttaggaaaca tgtcttttcg cgtcgtccct
     5281 accccagggc attctatagg aagcgtcagc tttatttttg atgattttgt cgtttctggc
     5341 gacgccctgt ttagaggaag tatcggtaga accgatctgt atacagggaa tttagaacaa
     5401 ttacttcata gtattcggac ccagctgttt gtattacctg atgaatttgc agtctatcca
     5461 ggacatggcg atgcaacgac gattgaacag gaaaaaagga caaatccttt tttcaatcgt
     5521 taaaacgact taggaggaga ataaaatgac agaaactaca ctttatatcg tacgacacgg
     5581 aaaaccgatg ttcaatacaa ttgaacgagt acaaggctgg tgtgatacac ccctcacaaa
     5641 gcaaggacaa gaaggcattc attatttagg tcgcgggtta aaaaatgtcg acttttcatt
     5701 tgcttattct agtgacagtg gtcgtgcaat cgaaactgcc cgtattattc taggagaaca
     5761 ttctaaggga aaagaaattc cttattttat tgaaaaaagg attagagaat ggtgtttcgg
     5821 gtctcttgaa ggtggatatg acatggaaat gtggggtgtg atccctcgtg tattgaattt
     5881 taaaacatat gatgaaatgt ttaccacaga agtttctttt gaacaaattg ctaatgctgt
     5941 atacaactta gataccgctg ggtgggcgga aacttatgaa acattgcgag accgtgtatg
     6001 gacaggattt gaagatatcg cccatcactg cgaaaaaaat ggtggtggaa atgttttagt
     6061 cgtatctcat ggtttgacga tcgccttttt gcttaatctg atcgatccta aacagcctgt
     6121 tcgtgctggg cttttaaatg gcagcgtaac aaaggtcact tatgcaaacg gtcgattttc
     6181 tatccacggc atcaatgata cgtcttatat cgataacggg aaaacacata gtcgcgactc
     6241 gtttctataa tggggatatt ttaattttta ccagagaatc ataaaaggca aagtctgaac
     6301 gagggctttg tcttcttttt tctcttcgtg tacttatctg ctttatttct caagtttttc
     6361 ttaatattaa aaaacagatt gggtttctca aaaaataacg gtatactata agatgtatct
     6421 taattgggaa ggaaggaatt tatgtttttt gcaaatatta tcaaagcagt gattctcggt
     6481 attattgaag gaataacgga atggctgccc atcagtagta ctggtcattt gatcctagca
     6541 gatgaattca tcaaactgga tgccagttca caatttatgt cgatgttcaa tgtcgttatc
     6601 caattagggg cgatcttggc tgtcgtcgtt ctttattttc acaagttaaa cccattttct
     6661 ccaagtaaat caacggtaga aaaaaaagat acgtggacac tttggtttaa agtgatcgtt
     6721 gcttgcatac cagccatcat cattggtttg cctttggatg attggttaga agcaaatttc
     6781 cacaagttct taccagttgc cattgttttg atcgtttacg gtattgcctt tattattgtc
     6841 gaaaaacgca acaaaaacaa aacacctcgt tggacttctc tgaatgactt tacgtaccaa
     6901 gcagctcttc ttgttgggtt attccaagtc ttggcacttg ttcccggaac atcccgttct
     6961 ggcgcaacga tcctaggatc tatcttgatt ggtgcttcgc gttttgtcgc tacagagttt
     7021 tcatttttct tagggattcc tgtcatgttt ggcgcaagcg gcttgaagat cgtcaaatat
     7081 ttagctgaag ggaattcatt caaaatggaa gaaactgttg ttcttcttgt aggaacattc
     7141 gtatcttttg tggtttccgt cttagctatc aaattcttac taagctattt gaagaaaaac
     7201 gactttaccg cttttggctg gtatcgtatt atccttggta tcattttgat tggttattgg
     7261 tttattgcaa tgtagaaaaa accagaacac ttctcttatt tgagaaatgt tctggttttt
     7321 tattttcttt aataataatc attataaata ataaaaacaa catcaagctt aatcaccaaa
     7381 tgataaccat tatcgttctc atttagctgt tattattatc aattacattt ttattatgct
     7441 atccttgttt tgacaaggag gtctaataac gatgactaat cagaaaaaag cttttgcagc
     7501 aacaatcggc tgccttttat ttatgatctt cggttttatc ttcgaccgat tctctcttac
     7561 catcacccca cttttttata tcagtgccat tatcatcggt ggatataaac aaacatcgga
     7621 aggcttgaaa gaattgtgga atgatcggac actgaatgtg gatttattga tggctttagc
     7681 tgctattggt gcctgtttga tcggtaatta ctttgaagga gcaatgttga cattcatctt
     7741 ttgtttaagc ggtgcattag aggaatacac aactaataaa agtcaaaaag aaattactgc
     7801 tttgatgaat cttcaaccac aaaaagccca attattaaaa gaaaatggac aaatgcaaga
     7861 agtagatgtt tctcaactaa aggtggataa cttggtattt gtcgctaaag gagcagctgt
     7921 tccgatagat ggctttttag aaagcacaca ggcaacgatc gatgagtctg cattaagcgg
     7981 tgaatctgtg cctgtagaaa aatatttagg cgatgagatg tatgcaggaa cgctgaatca
     8041 aggaaatccc atcacgattc gagtaacaaa aacgagttcc gaaactgtct ttgctaagat
     8101 tatccaatta gtagaagaag cgcagaatac gccaactcaa acagcttcat ttatcgaacg
     8161 aatcgaaaat aattatgtaa aattgatttt gcttgctgtt cctctaatga tccttctccc
     8221 ccactttttc ctaggttgga gttgggatga aagcttttat cgaggaatgg tattattagt
     8281 tgtggcttct ccttgtgcgc ttgtcgcatc tgctacacca gcttctctag ctgctctttc
     8341 aaattctgca aaaaatggga ttttaatcaa aggcggtatc catttagaaa agctttctga
     8401 attaaaagcg attgcctttg ataaaacagg aacgctcact aaaggaaaac cagttgtaac
     8461 tgacagcttt ttctttgatg aaaaggattt ggcacaacaa cttcttgttg ctatggaaca
     8521 aaaaacgacg catccacttg ctcatgcgat tttgcaatat ttcgattgca caatacctga
     8581 agaaatccaa tacttaccaa tagaagaaat tactggtttt ggattacaaa ctacctattt
     8641 aggtgcgcaa tggaaagttg gcaagcacgc atatgatcct gagacgatga taatcagcaa
     8701 agagattgcg gaaatgatcg aacgtttgga aaatcaaggc aaaaccgtga tttatctttc
     8761 taaagatcag cagttaattg ctgttttagg tctgctagat attccaaaag caaataccca
     8821 acaagtaatt tcctacttta aatcccaaaa catccataca agtatgataa ccggcgacca
     8881 ttctggcaca gcaaaggcca ttgctgaaca agtagggatt gaacattact atgcaagctg
     8941 tactccggaa gaaaaaacac aactagtcaa aaaagagaat gaactttacc aagtaaatgc
     9001 gatggttggt gatggagtca atgatgcgcc tgctttagca gccgcttctt taggtgtcgc
     9061 tatggggcaa gggacagatg ccgctatgga tatcgcagac atcgtcttaa tgaaaagcga
     9121 cttaaataaa tttgtctaca gtcatcggct atctttaaaa atgcggcgga tcatcaaaca
     9181 aaatattatt ttttcgattc ttgttattct cttgttgatt gcttctaact tcctgcaatt
     9241 tttaaatcta cctcttggag ttgtaggaca cgaaggaagc acaatattgg ttattctgaa
     9301 tggcttgcgt ttactaagac ctttacctga tctttctgat tcttcaaaaa aatgtaagtc
     9361 ttgtccacta tacaaagcgc ggctgtcttc tgtcaaataa gcaaagtgtt tttttatgat
     9421 tttgtttatt ttatgaaaaa aacagcttat gaataggtct tgaaggatga attcctactt
     9481 ttgtaacttt ctaaaaacat gttatgattg tttcgaagaa agtgaggaat atatttcatg
     9541 gaaagttggc tttttttagg aattatttta cttgtagcaa ttttagctaa aaatcaaagt
     9601 ctgatgatag cggcagcttt tgttttgatt atgaagctca taccagcctc ccgttccttt
     9661 ttcccttggc ttcaaggcaa aggaatcaat attggggtga cgatcatcac agcggctatt
     9721 ctaatcccta tcgcaacagg agaaatcggt ctacgtgatc tattagaagc ctttaaatcc
     9781 cctattggct gggtagctgt tttttgcggt gggctagtgg ctgtcttatc ttccaagggt
     9841 gtaggattga ttgcttctga tccacaaatc actgttgcac ttgttttcgg cacgatttta
     9901 ggtgttgtct tcttaagagg aatcgccgcc ggaccggtca ttgcttctgg tatcacttac
     9961 tgtatcttac aagtatttgc tttaggaaaa tagaacaaat tttgtctatt ttaggagatt
    10021 cttaacaccc ccaatttact gatccgtgat atactgacaa cgaggtgata caatgttcga
    10081 aaaattaaag tatttcatag gaacaatcgg ctggtttctt ctagcggtac ttttgattct
    10141 gggcttagct tatctattat ttcctgattt gatacaaaat gtgtttgata ctgctttttg
    10201 attgacctat taaaacgata taggggatgc tcctgcgatg caggggcttt ttttaatcaa
    10261 tgttttgtaa taatttaggc cgtgccaatg agacttccta tagaattcga tgctcttttt
    10321 tctttttggc tcttctgctc ttttttgtta tactagatta cagtggacaa aagaaagaat
    10381 gatcgcatcc acttcaacga atatcaaaat ggaagaatta gtgcctgttt tcaggttact
    10441 ttgaaaagaa aggaagacgc ttgtggaaac ttttgaaggt cttttaattg atacgtacgt
    10501 tttacaagaa cagccaatga tgctgactgg gatcaacttg cagcttgagg accgtgtgat
    10561 caatggcctg cttaaagatc gctatttggc aaaatatatc ttacgtttgc ccctaaataa
    10621 atatgtgctg gaagtaagtg gcaaatggaa ccgtcggaac cagttggtca taaaaaaaat
    10681 gttgataaaa aatatcgatg aatacattaa aataatcggt acaccagaat agcctttact
    10741 gatcaaagga tactggttat aaaataactg cagtttctta atagaaaaac aaagaacaac
    10801 agggaatcgt atcccttttc aaaaaaaact ttgcaaacgg ttctctttgt tatataataa
    10861 cttagtacca aacattccca ttcaaacttt gtttaaatag ataaataaaa atctagtttg
    10921 gtttgacgct attgcctccc acaatagcgt ctttttgatt tttttcagct tgtactatcc
    10981 gtttttactg aaaaaaaatc ctaataaaag actgttaagg aaaaaaactt gtcatgttca
    11041 tattgcaaag cacgctgcct ttaggtataa taatgaatgt tgaattatgc ggtaacacgc
    11101 gtaagaactt aagttatcgg aggtgaaact aatgccaaac attgaatctg caatcaaacg
    11161 tgttcgtaca agcgaaaacg ctaatgtgaa aaattcatct caaacaagcg ctatgcgtac
    11221 tgcaatcaaa aaatttgaag atgctgtagc ttcaggtgct gacaacgtag atgcgttata
    11281 taaagaagct gttaaagcta ttgatatggc tgaatcaaaa ggtctaatcc acaaaaacaa
    11341 agctaaccgc gacaaatctc gtttaagcaa aaaaatcgct aaataaaaga atcggcttcg
    11401 gccgattttt ttatttgtca aaaaaaggac aaaaacaaac gaccttcccg catgctttgc
    11461 cctcgtcttt cttcttatcg atttcttaac aactgccctg tgcgcaaaac gaatagttga
    11521 aaacttaatt ctttttcgat ttgtcccgtt ttgatttgat aatccatctc aactaattga
    11581 tcaaacaact gtaccagcaa atgttcatcg aattttttta cttcctgcat ggcaagtttt
    11641 acacgatatg gatggatttt caatgtttca gcaatatttg cctgctgata accaatcttt
    11701 acaagaaatt ttgtctgtaa aaataaacgt aattgagaaa gcaaaatcgc attaattttg
    11761 attgtttctt ccccttgcat aactaaatct tcgtataaac gcaaagcttg ttccgtcttc
    11821 ccttttaaaa tatattgggt catgtcaaaa atattatgtt ctaacgtttt aggaactaat
    11881 tcttctactt ctcttaccgt gatttgctgt tgattttgtc cataaaggac caatttctgt
    11941 aactctctca tcattttgga taaatctaag tcggtcaacc tcaagaataa atcaattgca
    12001 cttcgttcca ttttgattga ggaattctct aaggtattca tcagatattg acgtacttct
    12061 ttttcattca actgttggac attgatgata attgctgttt ttttcaattg tttcgttact
    12121 tttttccgtt catccaactt ttcatatggc gcaaagaata ctaatacagt cgattctaat
    12181 ggttctttca aataagccgt caataaatca gtattttgtt cgattccatt gtttactttt
    12241 tcacctgtta aaaaataagg attctctacg aatactaacc gttgatctcc gaaaaacggc
    12301 aacgtctctg cttcagctac tacctcatct aaacttgcat tttccatgtc aaaacttaaa
    12361 aaatttaagt catcttcgcc ctctaattgg atttttttga caatttctgt ccgaacttga
    12421 tcttggagaa acttttctgt tcctaaaatc aagtaacaag atgcaagttt ttctttacgt
    12481 attttttgta actctgcttg tatactcaaa ttactcacca ctttcaaaaa ttatcctacc
    12541 atgagcagat gagaattact agtctatcat tgtttccact agcccatgag gatgaaaaac
    12601 tggatgccag cgataggtga tcattccttg ttgatccgtt cgccatgttt ttatctgatt
    12661 ttcttctagt gtctccaata cttcgctgtg cgggtgacca taacgattat ttacgccgca
    12721 ggatataatc ccatgttttg cttcaatatg ggaaagaaaa tctgtgttac ttgacgtacg
    12781 gctaccgtga tgacctaatt tgactacatc ggctttcaga ttaggataaa tcaaaagcaa
    12841 ttgtttttcc attgcttgat tcaaatctcc taaaaataaa aagcgcctat tgttatattc
    12901 catccataga ccaatcgaat cttcatttct tccctctccc ttttcaaaag gagtcaatac
    12961 ataaagatca taataaccgc tgatcctgtc cccatgtttg atttcaatga tctcagtacc
    13021 atttttttga gcttcagata aatgcctttg taatggctca tttcgtccag cgccccaacc
    13081 gatataaatt tttttgatag taaaatgctg agataacgtg ggtaattctc ccacatgatc
    13141 cgtatcgtcg tgtgtcaaga tgagtttatc gattacactt atcccctgac cttttagata
    13201 tggaacgata tttctttcag caggaggacg tatcatacct tgcgcccact tttctctttc
    13261 aaagtgtaac tgacctccag tatcgatcaa aatcgtttct tttcgaaaag gactttgcaa
    13321 aaagatactg tctccttgac cgacatcgat aaaagcgatc gttgaagaaa agcgtaaaaa
    13381 tggcaaagtc aaaaaaataa aagaaaaaag accaacataa aacaagtgtt tcacaggttg
    13441 ttctttcaac ctatccatac aaatcagtaa tccgaaaata tacatcccag atagcacaag
    13501 gggcaaacgt cctataatga gagacgaccc tgaagaaaaa tcgattattc gctcaaataa
    13561 gattaatccc tcttctatca acaaaagtaa agtttccggc agccagcgat tgaagagtgt
    13621 gagaaataac acaccaggaa gaaaaagaaa cttgaatacc ggtgcaatca gtaatgtaaa
    13681 aagactacct aaaataggcc actcataaaa atgccataca atcaacggca tcgtgatcat
    13741 agtaaacagg acgtgagagg ccgttttttt caaccaatct ccatcaaaag aaatcaggat
    13801 aaacaaaaaa ctcatgaaaa aactaagctg tccacccgca tgaaaaagaa atcgaggatc
    13861 aaatagtaaa agaagccaaa agacaactgc aaataagtcc atggtggaat aatgcttatt
    13921 cagcaatttg actagcaatc gcaataaaaa cagcaaacta gcacgcaaaa cagaaataga
    13981 acttcctgca atagaaacat aacagatagt tagtagactc aacggaataa tactgacgtg
    14041 tagtggccat ccgatacgtc gaaataaata atacagccac cctaaataaa attggatgtg
    14101 tagacccgat agactgaaca gatgcagcaa accactctcc ctaaaaattt cttctggttc
    14161 tctgaatcct gcatctttat agccccataa taaagaatta atatatacac ttgttttttg
    14221 cggaaattca gattggacaa aggagataaa agatcctctt tttctctgaa gccagtttcg
    14281 ccagtgattt tgcatggtga tctgttcaat cttttgaatt tggaaccgtc cagaaattct
    14341 atggttcctg aagtaagctt cttggtcaaa attataaaga ttccgctttt ttgctggaga
    14401 gatgaactcg ccttcagcag tcaattctat tccctttcct cggaagttaa gccatgtttt
    14461 caattctgct tctgtgttta cttgataaga aagctggagc ttttgtttat tctctgataa
    14521 ccttccttcc attgtcaaaa a
//
LOCUS       DACEXQ010000043        14309 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.043, whole genome shotgun sequence.
ACCESSION   DACEXQ010000043 DACEXQ010000000
VERSION     DACEXQ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.043"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            349..1515
                     /locus_tag="IXH04_000994"
     CDS             349..1515
                     /locus_tag="IXH04_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1851621.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
     gene            1932..5159
                     /gene="fss3"
                     /locus_tag="IXH04_000995"
     CDS             1932..5159
                     /gene="fss3"
                     /locus_tag="IXH04_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAQ1851622.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVALVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVNCTVERHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            5272..5586
                     /locus_tag="IXH04_000996"
     CDS             5272..5586
                     /locus_tag="IXH04_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ1851623.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            5599..5973
                     /locus_tag="IXH04_000997"
     CDS             5599..5973
                     /locus_tag="IXH04_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ1851624.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            5983..6318
                     /locus_tag="IXH04_000998"
     CDS             5983..6318
                     /locus_tag="IXH04_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAQ1851625.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            6401..7417
                     /locus_tag="IXH04_000999"
     CDS             6401..7417
                     /locus_tag="IXH04_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ1851626.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFPDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMGANRFP
                     TSIE"
     gene            8011..9885
                     /gene="ltrA"
                     /locus_tag="IXH04_001000"
     CDS             8011..9885
                     /gene="ltrA"
                     /locus_tag="IXH04_001000"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ1851627.1"
                     /translation="MNVETDEVQGSGKNVGKSVMEVPRLNTKIRYWEYYDLQETFDQL
                     FTQSRNGKKFYQLYELIISENNILLAYRTIKANKGSSTPGTDSFTIDNYKEMNQAEFI
                     HLILSHLENYKPKSIKRVMIPKPNGEKRPLGIPCMIDRMIQQMFKQILEPICEAKFYE
                     HSYGFRPLRSAKHALGRIMYLINISKMHYAVDIDIKGFFDNVNHRLLIKQLWNIGICD
                     KQVLAILSKSLKSPIQGEGIPSKGTIQGGIISPLLSNVVLNDLDHWVSKQWHTFETKY
                     PYTKGYNKFRALRDTNLKQGYIVRYADDFKIMTNDYPTALKWFHAVKLYLKDRLKLDI
                     SNEKSKIVNLRKCKSEFLGFAICVKQKGKKWVCNSRISNKKKDQIKEEIKQRIKDIQK
                     SPTAQNALLFNALVLGVHNYFRYATHINLDLNRMNYVLSFTTYNRLSNCSTRCYPNKA
                     SPTYKKFYSTTMKTYKVAGVYLYPLCDVSTKTIYGFNTEDTPFTPFGRQRLEHKSLQS
                     LVFQELRKLMESKIPNRTVEYLDSRISRYSMKSGKCEITKQFLPAKAVHCHHYLPKSL
                     GGDDKFDNLRIIHKDIHLLIHTTNKMIIDHYVHELKLVPEQIAKINLYRKMCNLQNIQ
                     "
     gene            9957..10331
                     /locus_tag="IXH04_001001"
                     /pseudo
     CDS             9957..10331
                     /locus_tag="IXH04_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346898.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            10426..11101
                     /locus_tag="IXH04_001002"
                     /pseudo
     CDS             10426..11101
                     /locus_tag="IXH04_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            11334..12476
                     /gene="dcm"
                     /locus_tag="IXH04_001003"
     CDS             11334..12476
                     /gene="dcm"
                     /locus_tag="IXH04_001003"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAQ1851628.1"
                     /translation="MRFLDLFAGIGGFRLGMEQASHHCIGFCEIDKFARRSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AALLRPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYIPQSRERI
                     FLIGHSRDACTEQVFPIIGPSSTSDQNIRNLVNINPSNRGMGGQVYGSDGVAPTLTGD
                     EGIKIALPARDGISVAGLLPGKFEQGNRVYEVTGTAPTLSTKQGGTKIMIRKNETEYQ
                     EVKPGDSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAIVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVQKCDL"
     gene            12466..12756
                     /locus_tag="IXH04_001004"
     CDS             12466..12756
                     /locus_tag="IXH04_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851629.1"
                     /translation="MIYDELVGEIYWVIEKIQSDPELEEQLRRLNFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            13014..14198
                     /locus_tag="IXH04_001005"
     CDS             13014..14198
                     /locus_tag="IXH04_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ1851630.1"
                     /translation="MVQQNLDYRLLKDRRNQYGVSQNKLATACGLSRPYLNQIENGGV
                     TTSTKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDVQKIVQEILHLKFDYMLHED
                     YAFYSYQEQYIMGDIVVMLSHEEEKGVLLELKGRGCRQFETFLLAQKRSWYDFFDDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDKSETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIEGTTFSIINRYLRFTDKVEGKRRTNWPLNERWGRFIGRNRKEIQLTSDPKPYT
                     IERTLSWLGRQVAPTWKMAKELDRLNQTTYILDMVQNAQLSDRHKKILEQQSMAIENL
                     II"
     gene            14195..14293
                     /locus_tag="IXH04_001006"
     CDS             14195..14293
                     /locus_tag="IXH04_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAQ1851631.1"
                     /translation="MKRGSSMLGITVMCLIHTSAAADRVMERKVIK"
BASE COUNT         4892 a         2404 c         3025 g         3988 t
ORIGIN      
        1 gactcagatt attttactga attatctaat ctatactttt ccatgacttc tttacttgaa
       61 gtcaaacttc aagaagatga ctccgacttt ctttttttaa aaagtattct agaggactcc
      121 tttgaataca cgattctatt tgccaaacaa aagaagttta ttaataaagc tttaaactta
      181 aatatcgatg aaagaaaaga gttagaatct gagttatttg gcaacttaac ccaagtacag
      241 gttaacaatc taaaaagaga gataacagct cgattaatat caaaacttca ataaaactaa
      301 ttattcaatc agccaactcg ccaagttatc agattggaga gattagttat gtcaatacaa
      361 aagtacacaa caaaaagcgg tgcacgatat agtgtccgct tgtatgttcg taaagatgaa
      421 aatggaaagc aagaatacta tgtaaaaaaa gggtttaaaa ctgaaaaaga agccaagtta
      481 cacgaagcaa gaaaaaaggt cgagattgaa gataccggct atcataagcc ttctaatgac
      541 ttgtttataa cagtgtatga agaatggttg ccttcttata ggaatcgtgt acaagaaacg
      601 acatatcaac gtacaaaaga ccttttccgc ttgcatatac taccaaaatt cgggaataag
      661 tccatttcta agattactcc tgttcactgt cagaatgcag tcaatgaatg ggctttaaaa
      721 ttcaagaata tcaaacagct taagtcttac agctctcaaa tatttgagta tgccattttt
      781 gcagagctat tacaccgaaa tcctatggca aatacaaatc taccaaagcg ggaaaagaaa
      841 gaaaatgaaa attacttttc attagaagaa ctaaaagctt tttgggatat tctcaataaa
      901 gaagagcctt taaaacatat tttaatcttc cagcttttga ttactactgg tatacgtaaa
      961 ggagagctag ctgctcttca ttggtctgac atttattttg aagaacagct aatgtatgtt
     1021 agaaagagct atgcaactat tcgaaatgaa gagaagaatg caagaagaaa aactgtcagg
     1081 attcaaaaaa atacaaaaaa tatatcttcc gaacgaatat taccaattga tgcacaaact
     1141 attgaaatgt taaaaatctg gaaaaaagaa caagctcgag agcttttgca gtttggcata
     1201 aatacaaata caaaaaatca acttatcttt acctatgtaa atgcagacgg tgacattaat
     1261 caacctttgc atgcggacta ttcaaataat atattgaaac gtttagagaa gaaatataaa
     1321 tttaagcacg ttactattca tggattccgg cacactcacg ctactcttct actagaaggt
     1381 ggagcaagta tcaaagaaac acaagatcga ttgggtcata aaaatgctga aactaccctt
     1441 aacacttatt ctcatgttac tgaaaaagct caacgaaatg cgttagaaaa attcattgct
     1501 tacactggtt tataatattg tcattacctt tctcattacc agaacgaaaa aaagacacct
     1561 tctcgcaatg agaaagtgtc tgaaatcgtt ggtataacaa cgattaacgt tttgaaaact
     1621 gtgaagcttt acgtgctttc ttaagacctg gttttttctt cgtatcattg aataagtttg
     1681 aaaaaccttg atttataaag gttttcccgt aaatatattt tattatattt gattaaattt
     1741 gaaatgtttg tgttctaaaa tgtgttctaa tttaaaggca gtatatttcg tattttagat
     1801 taaattttta ggctcattca ttctcaaatc cattctattc agtcaataaa aattttaaaa
     1861 agactttata aacaaagatg ttcaagcgag catcttattt ttttgtgctt aatttgaaag
     1921 gagaacattt catgtataaa aaacaaaaaa agaaattatg tgtattaact attttgagtt
     1981 tggctgttca agcaattgtt ggagcaattg ctcctacgat tacttttgct gatgaaatta
     2041 cccatccaca aacggtaacc gtagaactag atttagcaca tcaatatgct gttgaaggaa
     2101 cttttagtga tggtcgtcca atgtcagaag tcactgttcc gcattatgca gtgtacaacg
     2161 gctttaaaca agatattttc tgtattgaac caggagtccc gatttacaat gagttcactc
     2221 ctggctatga gaaaaacccg ttaccagata tgtccgataa agccaaatta gtttccgttt
     2281 tatggaaaaa agtaggtaca gatgcagata ctcatattgt ggcacagaag atgatctggc
     2341 aagaagttaa tgggtatacc cttcactcaa taaaacgatc agatggtagt gccgtaaata
     2401 tcgcagccat tgaggcaaaa ataaatcaag ccattgacga ttatcagaag aaaccaacct
     2461 tccataatag tacggctaaa acagtattag ggcaatcgac tactgtgacg gatacgaata
     2521 atctgaattt gtcagagttc gatgaggttg tggaaaatgc cgcaaacatt gattatcgtg
     2581 tgaatggtaa tcagttgata atcaccccaa atgccaattc aaaagaaagt ggtgtgctga
     2641 cgcttaagaa atctgctggt accggaacac ctgtcgccta taaaaaggct ggccaacaga
     2701 ctttaatggc cggggcgatt gataaaccta atacctacac aatcaaaatt gatgtggaaa
     2761 cggagggttc tttaaagatc aaaaaagtcg ataaagaatc gggtgatgtt gtaccaggaa
     2821 cggttttcca tttagacttt ggaaaaactt tacctgcaaa agacgtgact actgacaaag
     2881 aaggcattgc tacattggat gagattcctc atggtacaaa agtaaccatt accgaaaaat
     2941 cagtaccggc gccttatacg attgatacca cccccatgac taccaccatt aaagcaggcg
     3001 aaaccattta cgtgacttca aaaaacgcac gggaaaaagg gcaaatcatt ctggataaaa
     3061 gcggagttga aacagggagt gatctttgga atgacaatta ttccttagct ggtaatacgt
     3121 ttgccattcg caaagacagt ccaaccggtg aaatcgttca agaaatgacc acggatgaaa
     3181 acggtcatgc ggaaacacca aaagagattg ctaatgcgtt ggaattggga acctattatg
     3241 tgaccgaaac taaagctagt catgggttcg tgaatacgtt caaacctgta aaagtcgaat
     3301 tgaagtatgc caatcaaacc gtagcccttg ttaccagtaa cgtaaaaggg caaaatcaag
     3361 aagtcactgg cgaaaccact ttgacgaaag aagacaaaga tactggggat aaagcacaag
     3421 gtaaggcagt ctttgagggt gctgaataca ccttgtttac tgctaaggat ggaaaagctg
     3481 ttaaatggag tgaggctttt aaaccagagt tagcgaaggg aacgaaaggt tctgatgaaa
     3541 cggtcacttt gactttagac gaaaagaacc aagttgccgt taaacattta gctatcaacg
     3601 agtattattg gcaagaaaca aaagcgcccg aaggatatag ccttgatgaa acaaagtatc
     3661 ctgtatccat caaaaaggtg gatgatagcg aaaaaaatgc cgtgattacc cgaaatgtta
     3721 cggcaaaaga acaagtgatt cgctttggct ttgatttctt taaatttgct ggatcagctg
     3781 cgggcactac cgaaactggc ttcaatgatc tgacctttaa agtgtcgcca ttggaaggga
     3841 ccaatgaaat cacaggtgcc gaagatgaag cgaccacagc ttataacgag caattagggt
     3901 ttgatggtta tggcaagttt gaaaatcttc cttatgggga ttatttactt gaagaagtag
     3961 aagccccaga aggttttcaa aagattacgc cattagaaat ccgttctact tttaaggaaa
     4021 acaaagaaga ctttgtgaag agtgagtatg tctttaccat taccgaacaa gaccaaaaac
     4081 aaccaattaa aacggtgacc gttccttacg agaagctgac gaacaaagca ttttctgtta
     4141 gtttgaaccg tttaatgctc tatgatttgc ctgaggaaga agatagttta acttctcttg
     4201 cgacttggaa agacggaaac aaagaattaa cttctctgga ttctaccgag ctagttgata
     4261 aattgagtta caatctacat gaaatcaaag aagactggta tgtggtagcg caagccattg
     4321 atgtggatgc gacaaaagct gcccaagaaa aagatgaaaa agccaaacca gtggtgattg
     4381 cagaaacaag cgcaacgttg gcgaacaaag agaaaacagg aacttggaaa attcagcata
     4441 aattaaccgc cgaacaagtt ttaaataaaa ctattgtctt attcaactat gtgtatgaaa
     4501 ataaggaagc ttttgaagca ggcgataagc cagtagcgaa ggatgttagc ttgaataatc
     4561 aagcccaaac tgttaattgt acggtggaac gtcatgtttc tatccaaaca aaggcccacc
     4621 tagaaaacgg ttctcagacc tttactcatg gtgatgtggt ggatatgttt gatgatgtgt
     4681 cgattactca tgatgtactg gatggctcaa aagaagcttt cgaaacaatt ctgtatgctt
     4741 tattaccgga tggtacaaac aaagaaattt ggaaatctgg caagattgat tatgaagtaa
     4801 atgataaaga attcaccaaa actgtgcttg cgaaaaaagt agataccgga aaatatccag
     4861 aaggaaccaa gtttactttt gcggaaatca attacgataa agatgggaat atcaatggaa
     4921 aacacaatga agatttgaaa gaaaaatctc aaaccttaac accaaaagaa gttccaacca
     4981 tactaagtac gccgaaacaa ccggaaacac caactgttcc aagtggctct caagaatcta
     5041 gtcccacagt gaagaccttc cctcaaaccg gggagaaaaa ttccaatgtt ttactgttca
     5101 ttggttttac tttgattttt gcgacggcgg gctattattt ctggaatcgc cggaactaag
     5161 gtgatgagat gatacaagta aatcctaaga accaacgtgc gcgaccgcct cctaaaaaag
     5221 tagtcgcgct atttttgtat ccaaaactaa caatgaaaag aggaaaagaa gatggaattg
     5281 aaatttgtcg tgccagatat ggctgaaacc tttggaaagt taagttatgc cggtgaagga
     5341 gaaattataa cagaaggata tggacggaat actacggtga ttggtcgcag ttatcatttg
     5401 tattccagta agcaacgggc cgatgatatt gaggtggtag tagctgcgga agccggtgaa
     5461 aaggattttg atcaagacca accgctaaaa gccgtgaatc cccatttggt tgccaaaggc
     5521 tatgagattg agaatcgtgg gtttactgat tacgtgttgt atgttgatga tttagtgaaa
     5581 gcataggagg aaaagaagat gagattaaca gaaggtattg tcgtagattc aggtttaaca
     5641 tttgggaagt tgcgtttttc cgcattacgc cgagaagtac ggaagcaaaa tgaggatgga
     5701 acaattggta atgaggtaaa agaacgaacc tataatttaa aatcctctgc acaagggcga
     5761 atgattcaag ttagtatccc agctaatgta cccttacgcg agtttgctta tgatgcagag
     5821 gtagagttgg tcaatccaat cgtggatacc gttgccaatt atgtttttcg agaaggaact
     5881 accgttaatt ggttcattaa ggcggatgat ttagtcttga agcggcagcc gaatcaagga
     5941 aatcctacga accaaaatga aagaaagaaa taggtgaaga ttatggaaat taatgttgaa
     6001 catattttag attgtctcga tcaatatgga aaaggcgagc tgactgagga acaactcgtt
     6061 aaagcattaa cctctgatga aaaaatgttt ctgattatac atcaaggttt acttggagaa
     6121 aatagcgaca gtgaagaaga ctctgatgta ctagactggt taggagaaga agagtctttc
     6181 tttatagttg tcgaagtaaa tgaaaactta tgtcggcagg cagaatccat tttggaagaa
     6241 attggtgtgg agatgccgga tgctattgaa ggctttttaa atcaattggt tgaaacgaaa
     6301 caactcccct cctcgtagca gtaaatgact agcataactt tttgaaattg cctaatgaaa
     6361 tcctattggg ctttttcgat tggaggtgga aaccatcaaa atgtacaaag gacatcgcat
     6421 acgcgcagga gatcagcact tggtttatca ctttgttctt ggctggctac tcgctctgtt
     6481 catcggttgg atgagcgtct tttattttca agagtttaga caatttgata tttctaagct
     6541 atcactttct actattgaaa tcgtttggtc tataaaagat ttagtatgtc tgcttggaag
     6601 tctcgctttt tcaggagcta tgatattgct ttatattcac ttttttcctg atcattggcg
     6661 aagcttgtgg catcgacaaa agctagctcg gatgattcta gagaaccatt ggtatgaagt
     6721 gaaacaaact caaagtgaag gctttttcaa agatttgaat agtagccgaa ccagagagac
     6781 tatcagttac ttccccaaaa tctattatcg aatgaaagat ggattactgt ctattcgcgt
     6841 tcaaatttca cttgggaaat accaagatca gcttttaaaa ttggaaaaga agttagaaag
     6901 cggactgtat tgtgagttag tagaaaagga actcaaagac tcctatgtgg agtatacttt
     6961 actgtatgat atgatcgcca atcgaattgg gatagacgaa gtagtggcag aaaatgggac
     7021 tctgcgattg atgaaaaatc aagtatgggc ttatgattct ttaccccata tgttaatcgc
     7081 tggtggcaca ggtggaggga agacctattt cctcctcacc attatcgaag cactattgaa
     7141 gtcagatgca gaattgttta tcctcgatcc caaaaatgca gatttagctg atttaggtac
     7201 ggtgatgcct catgtttatt cacaaaagga agaaatttct gcttgtgtgg aagatttcta
     7261 tgaacgtatg atggctcgta gcaaggcaat gaaagaaatg tctaactaca aaacaggaga
     7321 gaattatgcc tatcttggac ttccacccaa ctttttaatc tttgatgaat acgtggctta
     7381 tatgggtgcg aatcgttttc ctacaagcat cgaatagatg tgaaatggaa gcctattgag
     7441 attatcaata gtgacaacta atgttttgag agatggaatg aaagccttca agttgagctg
     7501 aaacatcccc gctaatactc ctacatgctc taatcaatga agtagattgg ttttggtatg
     7561 aagctaggtg aagtcgtctg aaatctgcct aagtgcttga gcataaggct gactataggg
     7621 cgggcggctg aaaaagataa ctatggtaag aatgtctgaa aatgactgac gaatctgcga
     7681 atgtacgggt ctaaaaggct gatcttattg aaagatagga acatacggac gtatggtgag
     7741 ataccgacaa gtaaggttcc atgatatgaa aatcgggata gctaacgaag aagctataat
     7801 ttcacaggct catagtagat acctaaggtt atacatgata gctaaaaaca ttggaacgat
     7861 gtaagcagaa aactatcaat taaatgacta cttctagata ggtgcatata agtaataacg
     7921 aaattgcttt gtttctgtga aagtaagggc acagtaccga tgaagcgtct aacaaatgtg
     7981 gagggatagc cctaagtcta tttaataaca atgaatgttg aaacagatga agttcaagga
     8041 tctggtaaga acgtgggaaa atccgttatg gaggtgccac gcttgaacac aaaaatacgt
     8101 tactgggaat attatgatct acaagaaaca ttcgatcagc tatttactca aagtagaaac
     8161 gggaaaaagt tttatcaatt atatgagtta atcatttctg aaaataatat tcttttagca
     8221 taccgtacta tcaaggcaaa caaggggtct tcgacaccag gaacggattc cttcacaatt
     8281 gataactata aggaaatgaa ccaagcagag tttatccatc ttattctcag tcatttagaa
     8341 aattacaaac caaaatccat aaagcgagta atgataccta aacccaatgg tgagaaacgc
     8401 ccattaggaa ttccatgcat gattgatcga atgatccaac agatgttcaa gcagatactg
     8461 gagccaatat gtgaagctaa gttttatgaa catagctacg gtttcagacc attgagaagt
     8521 gcaaagcatg ctctaggtcg cattatgtat ctcataaaca tcagtaaaat gcactatgca
     8581 gtagacattg atatcaaagg tttctttgat aatgtaaacc accgtttact gataaaacaa
     8641 ttatggaata taggaatttg tgacaaacaa gtcttagcta ttctttcaaa atctctgaaa
     8701 tcacccattc aaggagaagg tatcccgagc aaaggaacga tccaaggagg tatcatttct
     8761 ccactattat ctaatgttgt tctaaacgac ttagaccatt gggtctcaaa gcagtggcat
     8821 acctttgaaa caaagtatcc ctatactaaa ggttacaata aatttcgtgc tctaagagat
     8881 acaaatctaa agcaaggcta tattgtcagg tacgcagatg actttaaaat catgactaat
     8941 gattatccga cagcattaaa atggttccac gctgtcaagc tttacctaaa agacagactt
     9001 aaattagata tttccaatga gaagtctaaa atcgtcaatt tgcgtaaatg taagtcggaa
     9061 tttttaggat tcgctatttg cgtgaaacaa aaagggaaaa aatgggtttg taattcacgt
     9121 atttctaaca aaaaaaagga tcagattaaa gaagagatta aacaaagaat caaagatatt
     9181 caaaagagtc ctacagcaca aaatgctctc ctgtttaatg ctctcgtgct cggtgttcat
     9241 aattatttca ggtatgccac tcatattaat ttagatttga ataggatgaa ctacgttctt
     9301 tcattcacta cttacaaccg attatcaaat tgtagtacaa gatgttaccc caacaaagct
     9361 tccccaactt acaaaaagtt ttactcaaca acgatgaaaa cgtataaagt agctggagtc
     9421 tatttgtacc ctctttgcga cgtatctaca aaaacaattt acggatttaa tacggaggat
     9481 acaccgttta ctccgtttgg tcgtcaaaga ttggagcaca aaagcctaca gtctcttgtg
     9541 ttccaagaat taagaaaatt gatggaatcg aaaataccga atagaacggt tgagtattta
     9601 gatagtagaa tatctcgtta ctcaatgaaa tcaggcaagt gtgagattac aaaacaattt
     9661 ttgccagcaa aagccgttca ctgtcatcac tatttgccaa aaagtcttgg tggagacgac
     9721 aaattcgata acttgagaat tattcataag gatatccatc ttctgatcca tacaacaaat
     9781 aaaatgataa tcgatcacta cgtgcatgaa ctgaaactgg tgccagagca aatagcaaaa
     9841 atcaaccttt atcgaaagat gtgtaatctg caaaacattc aatagaaaat aaatagatgg
     9901 aacgccgtat gaggtgaaag tctcacgtac ggtgtggagc gggggaaaag atggagatta
     9961 tatcaaattc ttacctatcg ctatagatgt taacgacgaa agaaagtgcg gtgattttga
    10021 ataagctcaa acaaatcgtg atgttgggtc gtcaatctgg tttctttctg attttggctt
    10081 gccaaaggcc ggatgcgaaa tatttaggag acggaatccg cgatcaattc aactttcgcg
    10141 tggctttggg tcgtatgagt gaactcggat attccatgat gtttggggag gtcgaaaaaa
    10201 acttctttat gaagcacatc aaaggtcgag gttatgtgga tacaggtggc agtgtgatta
    10261 gtgagtttta tacgccactt gtaccaaaag ggtatgattt cttagattca attagtaaat
    10321 tagtaaatta gaaaagatga atgattgagt gatcttcaat actgacttta tacttcaagt
    10381 aacgtataat acttttatat agtaggaaga aaggggataa ttaatatgga aagtaatgat
    10441 tcttatttag agtatgcaaa agcaaataag gataagttta tttcttatat tatcaaaggg
    10501 aaatcccccg aagttggacg agacgcagtt tttatggcag ggagtcctgg agctggaaaa
    10561 tcagaggttg ccattggatt agctgagaat tatgataacc atgtgattat tgatgcggat
    10621 tactttcgca cccagtttcc tgattataat ggaaaaaaac tctagtctct ttcaaaaagc
    10681 atcttcttgg ttggtagagc aggcatttag atatgtctta gatcatggct attcttttat
    10741 tctggatgca acttttgcaa tattaagtgc tgaaaaaaat atctcgcggg cgttgaaaaa
    10801 acattttcga gtgacgattt tctacgttta tcaagaccct aaaatcgctt ggaaatttac
    10861 acaagaacgt gaaattgcag agggacgtca tgtgccgaaa aaggtattta ttaacgcttt
    10921 tttcaaatca agagagaaca ttgcaaaagt aaagagtaaa catccagatg tattgcttca
    10981 tatcataata aaagattata cgaataatat ttccgaagtt cattatgctg cggacaatct
    11041 agaattattt ttaccactag ggtatagtag gaaagatttg gaggttcaat tacatgactg
    11101 ataaagaaca agtaacgaaa attgtaaaga aatacaataa gagcattgcg gatttatcgg
    11161 aaaatgctac tgcaaaagag ttcaaaacgg tgattaaata tgtagccgat caggccaatg
    11221 aaaaacaacg gaaattagtt ggattaaata aaaaatagta ggacaaatta ataatgtatg
    11281 agccaagagc aaaatgttct tggctttttt ctttgcaatg aatgaggtaa tgtatgagat
    11341 ttttagattt attcgccggt attggtggct ttcgtttagg tatggaacag gcgagccatc
    11401 attgtatcgg tttttgtgaa attgataaat ttgctagacg tagttataaa gcaattcatg
    11461 atacaagtaa ggaggtggaa atgcatgaca tcacaagtgt atcagatgag tttattcaat
    11521 ccctcggacc agttgacatc ctttgcggcg gatttccgtg tcaagctttt tcaattgcgg
    11581 gaaagcgaca aggattttcc gatactcgag gtactttatt ctttgaaatc gctcggttcg
    11641 ccgctcttct ccgacctaag tttttattcc ttgagaacgt ccggggattg ctcaatcacg
    11701 aagggggggc tacgttcgag acgatcctcc gaacgctgga tggattgggg tatgacgtgg
    11761 agtggcaggt gcttaactca aaggcctaca ttccccaaag ccgggagcgt atcttcctta
    11821 tcggacattc tcgagacgca tgtaccgaac aagtatttcc tatcattgga ccgtcttcaa
    11881 catctgatca aaacatcaga aatttggtaa atatcaatcc ttccaatcga ggaatgggtg
    11941 gacaagtcta tggttcagat ggcgtagcgc caaccttaac aggtgatgaa ggaatcaaaa
    12001 ttgcactgcc agcaagggat ggaatctctg tagcaggact attaccggga aagtttgaac
    12061 aaggtaatcg agtttatgaa gttactggaa ccgcccccac cttgtccacg aaacaaggtg
    12121 gtacaaaaat catgattcga aaaaatgaaa cagaatatca agaggtgaag ccaggagaca
    12181 gtgtgaacct ggcttttcct aattccacaa gtcgccgagg tcgtctggga aaacagagtg
    12241 ttcatacttt actgacagga gaccaacaag cgattgtcac ggatcagtat caaattcgga
    12301 agctaacacc tagagagtgt tggcgacttc agggctttcc cgattgggct tttgatcgag
    12361 cttctcaagt caattcagat agtcagttgt ataagcaagc cggaaactct gtaacagttc
    12421 cggtcatttt tgatattgcc agaagattaa aggaggtaca aaagtgtgat ttatgatgag
    12481 ttggttggag aaatttattg ggtgattgaa aagattcagt cagacccaga attagaagaa
    12541 caattgcgac ggttaaactt tgatattcgc aagaatggtg tcaaagtcgc tggcgatcca
    12601 tatttaatga atgaggagac ggatgctcga attgaaataa accaagtaat tgcagaattt
    12661 gagcggattg cggaccaagc aaaagaaccg gatattcgtc aatacttgtt agaaatgaaa
    12721 gcagagttgg aagtaaatgg tattacagat gagtaaccta aaccaacaca actgactgtt
    12781 aggcgctgac cgcggaggat tcgcgaagca gaaatcctcc gtggtgctgt gcctgttcat
    12841 ggacggcgtt agccggccat gatgtctcac cccccgtatc taacaggggg gtacaaaaac
    12901 acaaaaaaca agcaagcagc gattaagaat ggttgtcatg acgaggatag ttagctgttt
    12961 gaacaggtgt taactcattt tgaaaagtta acaccttatt tggaggtgaa aacttggtac
    13021 aacagaactt agattatcgt ttactaaaag atcggcgcaa tcaatatggt gtttctcaaa
    13081 ataaattggc tactgcttgt ggattgagtc gtccctattt gaatcaaatc gaaaatggtg
    13141 gcgtaactac atctacaaaa actatgagaa aaatttttga ccaactggaa agtttcaatc
    13201 ccgatttgcc tttgacgtta ctgtttgatt atgtaaggat tcgttttccg acaacggatg
    13261 ttcaaaaaat tgttcaagag attttgcatt tgaaatttga ctatatgctt catgaagact
    13321 atgcctttta ttcgtaccaa gaacaataca tcatgggtga tattgtggtg atgctttctc
    13381 atgaggaaga gaaaggcgtt cttttagaat taaaaggtcg tggttgtcgg cagtttgaaa
    13441 cttttttgct cgctcaaaag cgtagctggt acgacttttt cgatgattgc ttaaaagctg
    13501 gaggcgtcat gaaacgcttg gatttggcaa tcaatgatcg agttggctta ttggatattc
    13561 ctgagttgac gaaaaaatgt cagaaggaag aatgtatctc cttattccgg acttttaaga
    13621 gttaccgctc gggtgaactt ttgaaagctg atgaaaagga tggaatggga aatactttgt
    13681 atatcggaag ccttaagagt gaggtttatt tttgtttata cgaaaaagac tatgaacaat
    13741 atatcaaatt gggaattccg ttagacaaga gcgaaacgaa gaatcgcttt gaaattcgat
    13801 tgaaaaatga ccgtgcctac catgcaatac aagacttgtt aaaaggtcga agtattgagg
    13861 gtaccacatt ttccattatc aaccgttact tacggttcac ggataaagtt gaagggaaac
    13921 ggcgaacaaa ctggccgcta aatgaacgat ggggtcgctt tatcgggcga aatcgcaagg
    13981 aaattcaact gacttctgac cccaaacctt atactatcga gcgaacactg agctggctag
    14041 gtcgacaggt agcacctacg tggaagatgg ctaaggagtt agatcggtta aatcagacga
    14101 cttatattct tgatatggta cagaatgctc agctatcgga tcggcataaa aagattttgg
    14161 aacaacaaag tatggcaata gaaaacttga ttatatgaaa agaggaagca gtatgcttgg
    14221 tattaccgtg atgtgcttaa ttcatacaag tgcggctgca gatcgagtaa tggaaaggaa
    14281 agtgataaaa tgaatttcgg acaaaattt
//
LOCUS       DACEXQ010000044        14085 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.044, whole genome shotgun sequence.
ACCESSION   DACEXQ010000044 DACEXQ010000000
VERSION     DACEXQ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.044"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..637
                     /locus_tag="IXH04_001007"
     CDS             <1..637
                     /locus_tag="IXH04_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ1851632.1"
                     /translation="IPNGIHHPPIEDGRLLPNSVKLASRIAIMSEGRVIQFDTPENIL
                     RHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMREKRVDT
                     LLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQRILKRGL
                     KYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            641..1282
                     /locus_tag="IXH04_001008"
     CDS             641..1282
                     /locus_tag="IXH04_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1851633.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            1279..2196
                     /locus_tag="IXH04_001009"
     CDS             1279..2196
                     /locus_tag="IXH04_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1851634.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            2193..2864
                     /locus_tag="IXH04_001010"
     CDS             2193..2864
                     /locus_tag="IXH04_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1851635.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(3405..3893)
                     /locus_tag="IXH04_001011"
     CDS             complement(3405..3893)
                     /locus_tag="IXH04_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAQ1851636.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(4094..4360)
                     /locus_tag="IXH04_001012"
     CDS             complement(4094..4360)
                     /locus_tag="IXH04_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ1851637.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(4360..4623)
                     /locus_tag="IXH04_001013"
     CDS             complement(4360..4623)
                     /locus_tag="IXH04_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ1851638.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(4616..4888)
                     /locus_tag="IXH04_001014"
     CDS             complement(4616..4888)
                     /locus_tag="IXH04_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851639.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            5099..5284
                     /locus_tag="IXH04_001015"
     CDS             5099..5284
                     /locus_tag="IXH04_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1851640.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(5426..7249)
                     /locus_tag="IXH04_001016"
     CDS             complement(5426..7249)
                     /locus_tag="IXH04_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAQ1851641.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(7847..10552)
                     /locus_tag="IXH04_001017"
     CDS             complement(7847..10552)
                     /locus_tag="IXH04_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAQ1851642.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(10858..11805)
                     /locus_tag="IXH04_001018"
     CDS             complement(10858..11805)
                     /locus_tag="IXH04_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAQ1851643.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            11995..12264
                     /gene="rpsN"
                     /locus_tag="IXH04_001019"
     CDS             11995..12264
                     /gene="rpsN"
                     /locus_tag="IXH04_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAQ1851644.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(12440..12802)
                     /locus_tag="IXH04_001020"
     CDS             complement(12440..12802)
                     /locus_tag="IXH04_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ1851645.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(12813..13934)
                     /locus_tag="IXH04_001021"
     CDS             complement(12813..13934)
                     /locus_tag="IXH04_001021"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAQ1851646.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(13950..>14085)
                     /locus_tag="IXH04_001022"
     CDS             complement(13950..>14085)
                     /locus_tag="IXH04_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAQ1851647.1"
                     /translation="FQDVEILKNENGQPVVTRSPHEGNVWVSITHTNETAFAQIILED
                     "
BASE COUNT         3977 a         2993 c         2653 g         4462 t
ORIGIN      
        1 cataccgaac ggcattcatc acccacctat agaggatggg cgacttctgc ctaattctgt
       61 taaactagca agccgtatcg ccatcatgag tgaaggccgt gtgatccaat ttgatacacc
      121 agaaaatatt ttacgtcatc ctgcaaacga tttcgtagaa gagttgattg gagaagatcg
      181 cctactacaa gccaagccaa atgcgacaag agtcggcgaa gttatgctaa atacagcaat
      241 cacgattaca ccggaaaaat cattacaaga agcaatcaaa ctgatgaggg aaaaacgtgt
      301 agatacgttg ttagttaccg ataattcgaa tgtattaaaa ggatttatcg atgtagaaac
      361 gttgaataaa aaacgaggaa aagtatccag tgtcggcgac attttgaata aacatgtttt
      421 ttacgtaaaa gaatccgcct atcttcgtga taccttgcaa cgcattttaa aacgcgggct
      481 gaaatacgtg ccggtcgttg atgaacagaa caaagtcgta ggtatcctga ctcgtgcatc
      541 attagtggat atcgtctacg atgtgatttg gggagaagat cccgcaacta ctgatgttgc
      601 agagtcagct actcctgaag aatctaagga ggtctgacct atgcagcagt tttttcatca
      661 gcacggttca gaaattctct caaaaggttt tgaacatctt tatatttcag gacttgcttt
      721 gatcattggt atcatcattg ctgtaccgct tggtgtctta ctgacacgtt ttccaaaagc
      781 tgccacgatt gtgattggct taaccagtgc cttgcagaca atcccttctc ttgctttatt
      841 ggcattgatg atcccgctat ttggcgtagg taaattacct gcgattatcg cactctttat
      901 ttattcatta ttgccgattt tacgtaatac gtacattggg atgaaaaatg tcgattggaa
      961 ttatcgggat gtggcaaaag gcatggggat gactgagttc caatccattt tttccgttga
     1021 attgcccatt gctatgccaa cgatcatggc tggtattcgt ttagctgccg tttacgtgat
     1081 tgcatgggct acacttgcct cttatatcgg cgctggtggt ttaggcgatt tgatttttag
     1141 tggactgaat aattaccaac cagatctcat tttggcaggt acgatccctg taacaatttt
     1201 agcactattg gctgattggc ttctaggtct cttggaacac cggctgacac cgatcgcact
     1261 tagagaggag aatgaagaat gaaacgtata ctaaaaatga ccatggttct tttctctctt
     1321 cttatacttg cgagttgttc cttccctggt cttgccagca atacagatga agacaccatt
     1381 tcgatcacag gtggaatcac ttcagaagca cagatcttag caagtctagt tgccggaatg
     1441 gttgaacatt atacagataa aaataccgca atcatcaaca atctggctac tacaactatc
     1501 aaccatcaag cgatgatgaa cggagatgct tcaatttcag cggctcgcta cacaggaaca
     1561 gatttaacga ctacattgaa ccttcctcct gaaaaagatc cgaaaaaagc gtttgctacc
     1621 gtgaaagatg aatttgagaa aagatacggt caaacgtggt tcccgtccta tggttttgaa
     1681 aatacgtatg tctttcttgt tcgaaaagac accgcacaaa aataccatct atctaaagtc
     1741 agcgatctga aaaatgtagc ggatgaatta gtagctggtg tagatacttc ttggatcaat
     1801 cgtaaaggag acggttatga cggcttccaa gaaacttatg gcttctcctt caattcgata
     1861 ttacctatgc agattggact tgtttatgac gcagtcgaag caggaaaaat ggatattgtt
     1921 cttggctact ctacggatgg acggatcgcc agttatgatc tagtcatgtt ggaagatgat
     1981 cgtcaattct tcccaccata tgatgctgct ccagttgtag acaacaagat attagctgat
     2041 acgcctcatt taaaagaagc cttaaataag ttaggtcata ccatcagtac ggaaaaaatg
     2101 caggaattga actacgaagc agataataat ctagtggaac catcagtagt agcacaacgt
     2161 ttcttacaag aaaaccacta ttttgaaggg aagtgaagca attggataaa atgaatgtct
     2221 ttcaacaatt tttctattat ttccaggaaa atggctccta tatctttgca caatttatcc
     2281 gtcacttcct gatttctatc tatggtgtcc tctttgcagc agttgtcggg attccggttg
     2341 ggatcatgat ttctaggaga cgtaaattag caaactgggt gattcgtttg gcgaatatca
     2401 tccagacgat cccttctcta gccatgatct ccattttaat cattggatta ggcctgggag
     2461 tcaatgtcgt gattgttact gtatttttgt attccctctt accgatcatc aagaatacgt
     2521 ataccgggat gatccaagta gacaaaaaca tccttgatgt cggcaaaggc atggggatga
     2581 ctgctagaca acgactattc atggtagagt taccactctc tgtttctgtt atcatggcag
     2641 gcataagaaa cgcgttagtt gttgccatcg ggataaccgc gatcggtgca ttcgtcggtg
     2701 ctggtggtct tggagatatc attatccgag gaaccaatgc aacagagggc acatcgatta
     2761 ttttagcagg tgcattgcca actgcattaa tggcgatcat cacggactgg cttttaggta
     2821 ttttggaaag acggctagat ccagccagcc gtacatcacg ctgatgttat tgtaaaaaac
     2881 aaaagcttct gcgaccattt gttggtcgca aaggcttttt tagttctgtc atttttaagg
     2941 caataatcca ctctgattct tccctcctta tgggcaaaga gttgcttttc ttagctattt
     3001 atccattccg tatactccaa cttcatactg ccaacgggtt catatctgca tccaccgact
     3061 aagttttttt cttatccgct aaggcatttt gccaatccat tttcccatca ctcgtcatgg
     3121 aattcatacc acatgatctc taaatactcg tctgcttgtt ctttgaattg ggcatcaatt
     3181 cttacatcct ttggcgtggc tttcatttaa gccttttcca tcagtcctcg attgttcgat
     3241 gccttcagct gcatcatcaa aactggtgac tgccgtatcg gtaacttcga tcatctttta
     3301 tacaatcggg aacttgccat cctcatcctt ggctaaaaac atatctagcc attccagctt
     3361 ctcgccatgg gaggtttgcg gttcctaaaa ccggtacacg attcttatgg actgcatccg
     3421 tcgcaactag acttggcgat acgatgatcc ttcaccagaa gagcaccccc gtaaaccaat
     3481 tgatcgatat attgccaata agaaaagaca ttggcgtaaa agatatcgat ccctcgtgat
     3541 gcattgccgt tgatactgct attcatggcc gaaagcgcca cgactttaac ttcttccgtt
     3601 tgagtgacat tcatgttcgg caatgtcgct ttggataccc gctctgctaa aggaacgaca
     3661 ctggtattgt atttcgtatc tgtatctttt ccaaaagacc atgccaacga atcctctggg
     3721 aaccaataag acgactccgg ttgattgtcc attgcttttt tcaacatact tttggctgtt
     3781 tctcgataaa cgacatttcc aattgtcttc gcctcacttt ggcgataatc gatggtcgtt
     3841 gccactgcca acactaacaa cgatgctcca attttactga ttttccttgt cacacttctt
     3901 cctccttgtt ttacataaac ccgtcaacat tccattccta aactcaaaca atgacccttc
     3961 aaaggtggag atataggaac aaattaaaga aatcatgcaa taaatccaag aaggttggtt
     4021 ttagaacaaa aaaagagtct cctttttggg gagactcttt gttaactatc taaaaaggtt
     4081 ggctcttctc acttcactca taatgtgtcc aggcagaata aatttctaca actttatttt
     4141 cctcatcaac tttgtaaacc actcgatgct gtctattcag cctgcgtgag tatctgcctt
     4201 catgtgtagg tctcagcttt tcaaaagact gcgtgggcat atatggatct tccttcaatg
     4261 tctgaatcac ttcttcaaat tgtttcttta aattcgtttg cttgattttt cttaaatcca
     4321 ctttcgcaga gtttttgatt gcgactgtat agttactcat caaagagctt cccaatcaat
     4381 atcatcgata ttcgtaaagc tactgtcgtc tttttcgcgg tctcgtactt tatctaaagt
     4441 acctgtttgc tcaagaaaaa gggtttcttg aatcgctctc cagtcttcca gcccaatcaa
     4501 tacagcattg tttccactac gatcactaat aatctcgatt tcagtatgat tttcatttac
     4561 ctctttcaat aactgataaa aatttttacg agcagtgcta ggattcatcg ttgtattagc
     4621 cattgtctct cctccttttt ctgatataat tatagcgtac ttgattaagt acgtcaaaaa
     4681 aatacaataa agcacttaaa ttcagcaaac ttttcctctt ttttacatag agaaaaagac
     4741 ccctttttag ggaggatctt tgtccacgct cacttcttac tcctttacga ctagcggtag
     4801 ccaaacgtaa agtaattggt aacaacgtat catctttagc aaaggtcgaa tccaaagcct
     4861 gtgagtctat gcaacaacac caatacacga aggttcctcg ttgcagtgtg acttgattga
     4921 tatcttgaat aacctgagga tacgcacgaa agatttgtaa cggttattca aaactgattt
     4981 tggccgtcaa gtcttctact gcctcaaaaa tgattgcctc tttcgtctcc tgtcattcat
     5041 gttcactatc gatttgatac ttgcttagac cgttgggttt gtgaatcacc aattaaaaat
     5101 ggcactgagt tatggtaaca aaaaatggtc acttgacgag gctgagattc ctacttatga
     5161 agacacatgg cacttaagag tcgaggtaaa ggaagaagct gaaaatatag gaggctgtac
     5221 attatttggc aaaggattcg gaaattatga tcaagacatc aaaatcaaaa tgttttacag
     5281 ctaaatagga agaataataa catttcatac gctgttccca tctttaatgg cttatagagg
     5341 gcacctttag ctgagaataa gacaaaacag accctctagc ttcaattaag agctagaggg
     5401 tctgtttttg attttaaaac ttggtttatt tcttcaaatg gtagctatag gtgctgacaa
     5461 cgatattttc acgccaagac aatcgcaatc ttaaccgcaa acttgtttgg ttatggagaa
     5521 tattctgcca gcgtctgttt ggtacaaatt gaggaatcaa cacagtagtt gtatagtttt
     5581 gtttcgctgc atttttactt acgatatcta cataacggat aattggattt tcaatcgagc
     5641 gataagaaga gtggacgatt gaaaaacgga catctgggaa atgcttttta aattctgctt
     5701 cgatttcttt ttctttttcg acatcttcgt ctagcgaaac atgcatcgca acgacataat
     5761 caccgatcga acgtgcgtaa ttcaacgcac cgatatttac ccgtgtcaca ttcccgacta
     5821 atacaagaac ggtattcccg tcataatcat gtagttttgc atcattttct aaacgaagct
     5881 gttctgctac ttttttataa tgatcatgga tcttataaaa aatgaacata acgattggca
     5941 taatgatgaa gaaaggccaa atgtccccta accgatagac aaacaaaatc gcaatgattg
     6001 catatgagat gaacgcaccc gtaatattgg caatcgattt gcttaaccat ctttttcctt
     6061 cttttttcca tttgacgacc atccctgttt gagataaagc aaatggaatg aacacgccga
     6121 tagagtataa cggaatcaag cgttctgttg agccatggaa aataaacagc aatacgatcg
     6181 aacctagcgc tagtgtgata atcccattgg aatagcctag tcgatcccca cgatcttgat
     6241 acatgtgagg catgaattta tcttttgcca gattgtatgc aagaactgga aaagcagaaa
     6301 aaccggtgtt agcagcaact gctaaaatca gcgctgtagc aaattgaagg acgtaataca
     6361 aaataccatg tccaaagaca gctttaccaa tttgagacaa aacagtgact tctttttcag
     6421 gaacgatccc ataccaatag ttgataaatg tgatccccac gaagaagaaa cctaagatca
     6481 atgccatcat cgtcaatgta gcagcggcat tttttgctct tggcttctta aagaatggta
     6541 cagcattact aatcgcctcc accccagtta aagaggaaga accagaagaa aatgctcgta
     6601 aaatcaaagc gatagaaaca cccggcacag ccgctcctgg aagtgcagta gcttgcaatg
     6661 gcacagcacc tgtcacgata ttatataaac caacaacgat cagtaacgag atcacaagga
     6721 taaatgtgta caccggaaat aataggaacg atgcagattc cctcagccca cgaagattca
     6781 gcatcatcag tagcaaaacg attgtcacag aaatagcgac ttgatgaccg tagagtgctg
     6841 gcaccgctga tgtgatcgct tcagcacctg ctgagacaga tactgctact gtcaacatgt
     6901 agtcgatcaa taaagagcca ccggaaatca agccagcatt ctttccaaga ttctcactgc
     6961 tgacgacata tgctcccccg ccatgaggat aagcatggat gatttgacga tatgatagcg
     7021 ttaatgaaat caataaaata ataacaaaag cggcaatggg tagcgaatac cagatggctg
     7081 cagcagacaa agcgactaaa acgacaacga tttgttccgt accatatgca atagatgata
     7141 acgcatcaga cgacaataat gccaaagcag caaatcgggt taatttgtgc tcatcatttt
     7201 cagcagattt taaaggctta cctactaaca acctttttaa gtaatccaat ttctaaactc
     7261 ctttacacac aattttactt caagttttat agtcgaaaat ttatagtaaa caagatgtta
     7321 tagtacgctc actgacctta aaagtcaata aactttaaga aaataattgg aaaaaaacag
     7381 aaaaatatca agcgcttttt caaacgcttt aatacagcaa aaatctaaca tttttttatg
     7441 ggctttttct ttgaattcta taataaaagg atagaacttg acttagaaaa tcaagttcta
     7501 tcctttttga tatcttaact tttggaagag agatgtaagg ctgctttcta cccatcattc
     7561 ttactactcg aagcatagcg cagttgtcac aacctcttga taaacggtgt ttaaaagctt
     7621 gcatctgcgg taataagctc aaccaaatta aaaacataag aagctgtttt tatttggtcg
     7681 ttcttattac ttgatgcaga gcagcttttt tacaacctct tggtacacgg tgttcaatca
     7741 gctgaccctc ggtattagct caaaaaaacg gcaaaatatg aggaactatt tttccatttt
     7801 tttctctaat actcaggtca aaacactgat ttcacaacct ctttttttaa tgaaaaatat
     7861 ggctctcatg cgccgcttct ttgaatttcg tcattttttc ttcgatgaac ggatagattg
     7921 ccgttcctac gattccaaag acaacaaaca aaccgatcat gatccagatt gctttcgtag
     7981 catttggcca ataaattcct cctgcagatt cccgtaacaa gtttaccgca tgagtaaatg
     8041 gcaagaacgg attgatcatt tggaagaatt tccctgagac ttggatcgga tagttcccgc
     8101 ctccgccaga gatcgacagt actaagatga taatagcgat ccctttaccg acattatcga
     8161 ataatgcagc caagacatag acgatcatca tgaacgctaa ggcgatcaag acagcaaatg
     8221 acacgctgta gaccggattt tggacatcta ctcctagtaa gaaaatattc cctaatgtaa
     8281 cgatcaagct ttgcgcaatc gccattgtca aaaatgtcag catacgagca acgaattgtt
     8341 ctcgtttcgt aaaattgttt cggtctttct ttcctaaata gtattctgtc gtagcaacac
     8401 ttgaaagaag aacagcgccg acccacagac atagtgcagt ataaaacggg gtgctagctg
     8461 aaccgttatt tgcgattgga taaagcgcat tggtttgtag ctcaacaggt tgtgtaaaga
     8521 agtcactttc ttttgttgcg tctagtttca ataatttgat gacttcgcct agatcgactt
     8581 cttgttcgcc ttttcggatc gcgtctgctg ctttttggat accagctttg atatttggcc
     8641 agtcattgtt gatcaagtcc gcagcagcgt tcacagctgt ttcgacttct ggcatttttt
     8701 catttactgt tgtcaatgta ccggtgattt cattttttag tgctggccaa tcattttgga
     8761 taaaatcagc ggccaatcct aatttttgct gaatgatcgg taattcattg ttgtataggt
     8821 ctgccccttc attgatgcca ttgacgatgg tatccatatt tccattcagc attgtattgg
     8881 catcatggat ttcttgtcca attgcaggca tttctttttg atatttttct aaaattgcca
     8941 ctgcattcgt tacagttgct ttcgttgagc tgagaagcga atcaaagtcg atctggtccg
     9001 ctttgctcag tgcgccttgt gcagtcccaa tcgtatcgat gacttggtct agtagagaag
     9061 tgacagtgga actgatttga cctgtattga taccagcaat gatgccattg atattattaa
     9121 caacggcatc aatttgatct agcaattcat caatagcctt cgtgtcttca ttttgaacag
     9181 ctgtgttcaa agcagatagg cggttttgca gattgcttag caagggtttt aattgattta
     9241 gctgatcaat aattggctgt aatttcgtat ttccagcact tgtttgcaat ttagtcagga
     9301 actcgaccag tttatcgatt gcattttgtt gttgagatat tccgtcaata aagccgttca
     9361 atacattgct gatgtttttc ttttcatcat cagtcagctt accatcttca atagctgttc
     9421 ttacagtatc tattaacgaa cctgtcgttg ttgccacttg tccaattgac ttcaatgtaa
     9481 catctacact gtttgtgatg cttggtatcg catctttcag ctgagaggca cctgattttg
     9541 tttggtttgc tagatcactt gcctgatttt ctaaattttt cacatctggc aagatatctt
     9601 gtacttgttg gatgatcgtc aagccttgtt ttgcttcatc gattccttgg ttcattgtct
     9661 cttggatcga agcaaaatcg ccgtcgatct ctgctagttg cttaccggca ttttggatct
     9721 ctgggatttt ttcttgcaaa tcaaggatga tttttgcttg atctttcaac tcaggcattt
     9781 tgtcattcaa tgcaacgact ttttcaccca tctcatctac tttagggatg taatccacga
     9841 attcatttgc tttattcagt ttttccttga tttcaggtag ttgactttgt aattcgagga
     9901 cttgttttgt gtagccgtcg atcgtgtcca aattttcatc tgttgataaa atcatatctt
     9961 taactttatt gatacttact aggttcgtat cgatgtcgta acctatctca ttgaatactt
    10021 tcaataaagt cgaactggct gttttgataa attcatttgt gatttgcgat tggatcgaac
    10081 ttgctccttt atcggtgatt ttcggtgcaa tcgcattgat tttttcattt accgtatatt
    10141 cgatttttgg tttcttgata tcaccactag taaaactcag cagatcttct gagaaatcct
    10201 ttggcaagta aatacctgcg taatactttc cggaacggac tccgtcttct aattgttctt
    10261 ttgaatcaac gaattgccat cctaattgtt tatttttatg aagcgattcg atgacttgct
    10321 ccccaatggc cacttcttta ccttgaaact ctgctggctt gtctgcactg taaacagcaa
    10381 ttggcagctc tccagtattt ccatatggat cccataatgc tttgatgtta aaccacgcat
    10441 atagcgaagg gatgatcatg atcgcgatga tcagaagtgt tgcaatcggg tttttgaaaa
    10501 tccgtttcca gtcaagcaaa tataatcgaa gcgtattttt gattgactgc atagttctcc
    10561 cccttcttct tttttcagct gaattacttt accatgtatt tcatactttt ttcaatcggc
    10621 ttttttcaaa aaagtcgaat ttttggacat atcacggcaa ttgtctcatt gtaatctctt
    10681 acatattgag aaataattaa ggattacagc ctttacttta tcatgaaata aaagggaaag
    10741 ataattttat gccaaagttt ttatctatta tacgtatacg caaaaaagct aaaacatttt
    10801 ttgtctttgc ccctacaaaa atgtttcagc ttttttcgtt ttttattcct gtatttctta
    10861 tttttcgaat gacaggatga ttttcccagc aggaggatgt ccttcaacta gctgatgtgc
    10921 ttggatgaca tgcttaagtg aagcagacag attcattgaa ataaaaacat gaaaggctcc
    10981 tttttggtag gctttgatta tgccagcaaa ggcttctgca tctagatatt cctttcttgg
    11041 aacaaacgat tcataaaagc cttccttttt ttgccgcaaa tctagatctg ggaggtcatt
    11101 taatgcaaca tacctgccgc ctggtttcat gatctggatg ccggtttctc ctttgatact
    11161 tcctttcgtc gcatcgatta caaggtctgc ctgatcagca aattgcagac ctggatttgt
    11221 tttatcataa gcagcaaatg ctgaagctcc taattttttt accttttcct catttttact
    11281 ggatgcactg gttaagattc ggattccctt ttcatggaga agctggatca gacttgaccc
    11341 gaccgcacct gatgctccta gtatcattac agtatctgtc ggctggattt ccgtcaaatg
    11401 gttgattaga ttataagccg taattcctgg cgtgaccatt ccggctgctt cttcccaact
    11461 cattttatct ggaatttttg ctacttttgc tgatggcaac acgacttctt ctccgtaagt
    11521 cccgccgact gcatggacag ctactcgatc acctacatga acagttgtga catctgaacc
    11581 aatctctgtc acaataccag cgccatcatt acctggtaca taagggaatt tcaatgttcg
    11641 tatctctttc atttctccta aacgcaaggc aacatcataa ggattcacac tgaatgcttt
    11701 tatttctaca cgaacatgtc cagaaccgac ttctctggga tgagcatcga tttcttcaaa
    11761 aacatcttgt gcttctccgt aaccgttaat tgcaaatctt cgcatggtca ccatctccct
    11821 ttttctttaa gtttacgttt atgtctttat tttgacaaaa aaaatgtctt ttcgttttgg
    11881 cgcatgattc aaaacaaaat agttgtcttt tttcgcgtaa cgtgttaaat tatttatcta
    11941 aaagcgtaaa gattacgatt tacgctaata aaaaataatt ggaggaattt gttgatggct
    12001 aaaaaatcaa agattgccaa agcaaaaaaa cagcaagaaa cgattgaacg ctatgctgct
    12061 ttacgccagg aattaaaagc gcaaaaagat tacgaaggct tgcgtaaact tcctttagat
    12121 gctcgtccag aacgattgaa aaatcgtgat ttaatcgatg gacgccctcg cggatacatg
    12181 cgtaaattcg ggatgtcccg tataaaattc cgtgaattag cacataaagg tttgattcca
    12241 ggagtaaaaa aagccagctg gtaatccaag tctggctgta atgtaaagga ggagggcgca
    12301 tggaccactc cttggatcat caaaaaagat acgttcgctt cttctagcag aaatgctggt
    12361 actagaagca gacgtatctt tttttacgca cagctggtcc ttttttatat gtaaaggctg
    12421 catggttact tcgctatatt tatttagtaa caggagtcag tgcttcgata cccacactaa
    12481 ttttcaatgc tcggtcaact tctgccataa tgggtcgatc caaatggcag accttttctt
    12541 tcaatcttga tttatcgatc gttctgatct gctccaacag gataacggag tctctttcta
    12601 ttcctgttcc agtagctttg atcccaatgt gagtcggaag cttaggttta gccatttttg
    12661 ccgttatggc cgcaacaatg atcgtcgggc taaaatggtt acctagatta ttttgaataa
    12721 ccaaaactgg gcgtgttccg ccttgctccg atcccacgac tggagaaaga tcagcaaaat
    12781 aaatatctcc tctttttacc ataggatcct ccttattctt gatattctct tggtatccgc
    12841 tgtcctaatc cgcaagccac ttcatagtgg atcgtaccta attgatctgc tatatcctgc
    12901 atagtgattt ctttgtctgc atttttgccg ataagcgtca cttttgttcc gacaggaaat
    12961 tcttgaggca atcggatcat cagttgatcc atacatacac gcccaatcgt ttcacagtag
    13021 ttcccttcta ccaatagaga aaaaccctgc atctttcgtg gccagccatc tgcataaccg
    13081 atcggtatcg ttccgatcca ctctgcttca ggagtgatat atgtttctcc atagccgatt
    13141 ccttcacctg ctgggagttt tttgacttga atcaattcag aaaccagctc aagtgctggc
    13201 tgcaatggat aaacttccgg tagtgcgtgc ccagagggat taagtccgta cattgctact
    13261 ccataacgaa tcatattgcc gattgtttca tcatgccata aagccgttgc actattgctg
    13321 acatgtacat atcgaggaag ttcgggaagt tttttcagca cttctataaa gcgttctttt
    13381 tgcagattcc aatatgtatc atctgcctga tctgctgtag aaaaatgggt aaataaacct
    13441 tcccattcta atacacgact ttctttgatg aatgcagctg cctcttttac ttcttcaggt
    13501 gtacaaaatc cgatacgacc catcccagtg tctgctttta gatgaatcga aagaggcgtc
    13561 tctagcttac ttttctctaa aacagcagct gctgcttgca accattcccg tgtacctgca
    13621 gttactgata aatcatgagc aacagctaca gtcagtgcgt cgtaaggaac catattcaag
    13681 ataaggatgg gctgagtaaa tccagcttct ctcaactcga ctccttcatc taaatttgaa
    13741 acacaaaagc ccgaagctcc acctgcaact gctgcctcag ctgtttcaat agctccatga
    13801 ccgtatccgt tcgcttttac tactgcaaac aattcttttc cttgcggaag tcttgcaact
    13861 tcatttgcta cattttcagt tatagctttt ttatgtatca ctgctttcgt tgggcggtgc
    13921 caagcgacaa ccatcgtttt tccttctttc tagtcttcca aaataatttg tgcaaaagct
    13981 gtttcatttg tatgagtaat actgacccat acattgcctt catgaggtga acgcgtcaca
    14041 acaggctgac cattttcatt ttttaaaatc tcgacatctt gaaag
//
LOCUS       DACEXQ010000045        13993 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.045, whole genome shotgun sequence.
ACCESSION   DACEXQ010000045 DACEXQ010000000
VERSION     DACEXQ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.045"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            97..2424
                     /locus_tag="IXH04_001023"
     CDS             97..2424
                     /locus_tag="IXH04_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1851648.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSATETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2550..2879
                     /locus_tag="IXH04_001024"
     CDS             2550..2879
                     /locus_tag="IXH04_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAQ1851649.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     AEIKTSD"
     gene            2919..3923
                     /locus_tag="IXH04_001025"
     CDS             2919..3923
                     /locus_tag="IXH04_001025"
                     /inference="COORDINATES: protein motif:HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ1851650.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKTKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            3920..5349
                     /locus_tag="IXH04_001026"
                     /pseudo
     CDS             3920..5349
                     /locus_tag="IXH04_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
     gene            5555..6727
                     /locus_tag="IXH04_001027"
     CDS             5555..6727
                     /locus_tag="IXH04_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1851651.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(6889..7344)
                     /locus_tag="IXH04_001028"
     CDS             complement(6889..7344)
                     /locus_tag="IXH04_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851652.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQAAAEQASQQAQAAAP
                     EQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSKCYGN"
     gene            7933..8355
                     /locus_tag="IXH04_001029"
     CDS             7933..8355
                     /locus_tag="IXH04_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1851653.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            complement(8521..9714)
                     /locus_tag="IXH04_001030"
     CDS             complement(8521..9714)
                     /locus_tag="IXH04_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1851654.1"
                     /translation="MTTYQRKLLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            9938..10315
                     /locus_tag="IXH04_001031"
     CDS             9938..10315
                     /locus_tag="IXH04_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1851655.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            10303..10641
                     /locus_tag="IXH04_001032"
     CDS             10303..10641
                     /locus_tag="IXH04_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1851656.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            10775..11218
                     /locus_tag="IXH04_001033"
     CDS             10775..11218
                     /locus_tag="IXH04_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAQ1851657.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            11480..12295
                     /locus_tag="IXH04_001034"
     CDS             11480..12295
                     /locus_tag="IXH04_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1851658.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            12305..12967
                     /locus_tag="IXH04_001035"
     CDS             12305..12967
                     /locus_tag="IXH04_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ1851659.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            13172..>13993
                     /locus_tag="IXH04_001036"
     CDS             13172..>13993
                     /locus_tag="IXH04_001036"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="catalyzes the formation of 2-acetolactate from
                     pyruvate in stationary phase; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAQ1851660.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVI"
BASE COUNT         4738 a         2510 c         2742 g         4003 t
ORIGIN      
        1 aaagccccaa actttctata catcaataaa ataataaaaa ttaaatttag aataacgtgt
       61 aaaggattat ttgatgattt tttattgaga ggagggatga aaatgattaa gaaaactcaa
      121 aataaaatcc aacgtatatg tcaaaaacat tttgtttcta gactcaaagc tgtatctaaa
      181 agaaaatact tacatcccat tatgatttcg atcatcttga tttttacttt cttagggagc
      241 ttatcaagta tttatgtaaa cgtttataca gaaacgtcaa attttgctaa ccaaacgaca
      301 gaggagacaa atagtccacc ctattctgaa atacagtcca tagaaagcac caatcagtct
      361 agcaacaacg agacaacctc aaccacatcc tcgactcaac tgattgatcc gacagaaaaa
      421 gaaatcacag cagcaaaagc agcaggggag aaagcttatt cagaaacagg tagaagccag
      481 acatttagta ctgttgcggc cactgcgaat ttcaacggac ataccatacc agatggatat
      541 acagtgatat atgccaaaga tggagtcatt gttgcggtta acggatcgat gagtgcggat
      601 aaaactgcgt tacaaggcgt aaccaaagca atcgctttta ttgataaccg tccagatatc
      661 cataatctgt ttattggctc cacagtaaat ggtactacta tcacattccc aaaaagtatc
      721 tatatggggg tggctaccgc tggaaatacg atcgattcag ttacacccaa tgcggataaa
      781 agttattctt ttacaccctc aacatgggga gggcttcctg ccggaggagg tatgagtact
      841 ttttcaatcg gtatacagtt agcaaatgat attccattca cggggtactc gatcaaaaat
      901 gggcagatcg ttgatggtaa gggaaatgtt tcaaattttc caacctatca agcagcaacg
      961 gttcaggtca atgcgaatcg gacaggaact ttttcttata cagtcaatgg tggagcagca
     1021 aaaatcggaa cgtacgggac tacattatct ggaatccatg tgggtgacaa agtggttatc
     1081 actccaacag cagtaacggg ctattctttt gcacaaacaa acggttcttt cacagcccta
     1141 gaaagtaacg tcgatacggt gacctataca ggcaaacaaa caccaatcac tattaagtat
     1201 caagactatt ttggacagat gattgctcct tctaaaataa tgaatcttac ttacggaagt
     1261 gctgcacaag acctgacgac taatacgcct actatcaacg gatatacctt tacagctgtt
     1321 tcagcgacag aaacaaagaa ccaatcagca atgagtgttt tagctagttt agatacaaat
     1381 ggaaatgtaa tcgtaaagga cgcaaatgga aaacagattt ctgaagtcat cctatattat
     1441 aagacaaatg caacagtatc aatcaatgca aatggcagta agtactatga tggtctttct
     1501 gttgtaccga tcgttaaata tgcctttaat gataaaacag aatcaacatc ccttttaaac
     1561 aatgatctca gtattgatca aatcacatgg aatccaactg atttcaaagc gatgaatgaa
     1621 aagggagttg aagtaactgc gcctactgaa attggaacct atacgtcttg gcaattgaca
     1681 aaaagcggac tagctaaact agctgccaga acaaactatt tatttactat cgtgcagaca
     1741 tctgatgtct atacgattaa gcaaatcagc ggtgcggtag agctcagtgg cagtaagaca
     1801 tatgatggaa aagcaggtgt cccgtcaatc catgtcaagc tagcagaggg tgtcactagt
     1861 tcgcttactc cagtacaagt ggcacttagc agtatggatt acacagcaga tgcacaatca
     1921 gcaaaaaata tggttaatgc tggcagctat acaattaaac taacgaatag cggaatagac
     1981 aaagtcaaag cagcaaatcc caagttgtca tttactgata tagccaacac atcaggaact
     2041 tataccatct ataaagctaa cgcagtgatt actgtcgatg acgctgcctt taattatgat
     2101 gcccaaagtc actcgatttc ggttggaaat ctccacgtta ccggtgttgt cttaggcgaa
     2161 tcattggatt ataccctaac agttaacagc cggacagata ttggatcaca aatagtaacg
     2221 attagtttag gcgaaggaag tgtaaacaat aattatacga ttatcgcaac agataccgct
     2281 aagttgacga tcaatccgac tctcttgctt ccttcgacag gtgggatagg tctactgccc
     2341 tttgtctttt taggactgat ctttattttt tctggcttct tttattttat ccatcgtaag
     2401 aaaaaagcag gtgaacagcg atgaaaaaaa caaaaaatgt acgtgtggat atatttgctg
     2461 ttctcttttt tatcctactg gtctttttta taggttctcc gactgtgtca gctagagaat
     2521 cacctattac atttactttg cataatcgaa tgttcgaaag cgataagatg ccaagtatca
     2581 gtcaaaacga cggaataaat atcccggata actttcaagg agtaaaaggg ttgaacggcg
     2641 ttactttcgc tatatatgat gtctcggatg aattttacaa gctgcggtca gaaggaagta
     2701 gtgtggaaga cgcacaaaga aaactagcac aaaaaagtga tagtgaaaaa atactggcag
     2761 aaaatgtgac aaaaacagta gacgaggaag agggcattgc ttcttttagt gcatcggaca
     2821 aagatgagca aggtcgtgac gctgtttacc gatttgcgga aataaagact tcagattaag
     2881 taaaagaaaa atcagcacca tttgtcgttg tgcttcctgt gacagattca tctaatcata
     2941 aattgacgac catccacttg tatccaaaga gcgaacaaaa aatacctgct gcactcacat
     3001 taacaaaaac agtagagaat aagcagacag attttgcaga tggggataaa gttccttatc
     3061 tcatcaccgt aacaattcct gagaacatca acgagatagg aacctatacg atcaaagata
     3121 cagctgatcc tcagttatgg ctagaaccag aaacgatcga ttttttgatt ggtggagaca
     3181 agattcatac atttcacaca caaaaaacaa aacatggttt tgtcttatca gattctggaa
     3241 aagatcttag ttcgtttgct gggaaaaaat taacgatccg ttaccaaatg acattgaaag
     3301 aaaacagtga aaaaacgaca tttgataacg atgtacgttt gacgactgac aatcaaacag
     3361 tagaaacaca tcttgccatc caaacaggag gcaaacaatt tgtcaaagtg gatcggcaaa
     3421 cgaaacaaaa actagcagat gctcaatttc tagtcaaaaa aaatgaccaa tacttagcgc
     3481 aagaaaacgg gataaatcat tgggtagcca aagatgattc aagagcaacg atttttactt
     3541 ccagtaaaga tggaacattt tctgtccatg gattgtcttt tggaagttac gaactagtgg
     3601 agattaaacc gcctaaagga tatatattga gtcaatcaac cattctcttt gaagttgaaa
     3661 aaggcagtta tgatccgctt catcctatac cattggaaat catcaacgaa ccaaaagcaa
     3721 atccgacgaa taatggaaaa acgccagttc agatgcagac aaatggtcca tcaaatgaaa
     3781 aaaatggagg aagctttcca aaaacaagcg aagagatgct tggaggtttc tcaattttag
     3841 ggttgttgct agtcctttca acagggacag cttggtttta taaaaaacaa caaaaaggaa
     3901 acaataggag ggaaataaaa tgaaaaacat caaaacaaaa atatggacgt ttttaggaac
     3961 tgctatcatg ctgcttcctt tcgttctcgg cctaggaact gcagaggttt ctaggccgta
     4021 tcacctacac cagaaaatgt tacggttaat ttgcataaat tgaaattcac ttcagcacca
     4081 gaaaatcaaa taaacaacgg aacggaattg acatttccga atagcgaacc gttgaacggc
     4141 gttgaattca acgtatatga catcactgct acgtactatc caagtaaaga cacagcagtt
     4201 ccagcagacg ctacaccctt cgcaagtgta acgacttctg gtgaaggatt agctaacctg
     4261 actcttccag gtaaatctga tgggaaagat gctgtatacg tctttgtaga aacaccaaag
     4321 cccggtgtcg aaacgtcgcc aaatatcgtg ttaagcttac ccttttataa cccgaattca
     4381 tcagaccctg acaaaccttt agacacgatt cacctatatg ctaaaaatgt catcaaagaa
     4441 agcaatatca aaatccagaa agctgggaac ttttcagggg tagataagtt agcaggggcg
     4501 caattccaga tcaaaaattc agagaatcaa ttcgtgaccg gttttgatga aaacactggt
     4561 gtcgctctct atgatcctgc tgagtcagcg ttgacattca caactgatac tgaaggatcc
     4621 atcagtgtca atggattaat agacggcaat tatactttag ttgaaacaaa agcaccagat
     4681 ggttatcaat taaccaatcc acaaaaagaa actactttta tagtaaaaaa cggaaaattg
     4741 caacaaacaa ccggtatcat ccatggcgca gatgaagcca atcttgggaa tccaaacggt
     4801 gtagtcaatc gggaaaatac aactacggat aacacgatta ctgtagaaaa tgtccaaaac
     4861 tacggtgatt tccacttcat caaacaagat gtgaacacgc aagaaaaatt agccgatgca
     4921 gaattttcta ttctaagagc aaaagatcca actgttaaat taatgaaaaa aatagcaaat
     4981 cttgcctcag gagaataccc atatgcttgg tcagatgaag tcacaggttc tgactgggaa
     5041 gaagtgaaat tgatttctga ttcagaaggg aaattttcaa tcgaagggct gaaacaaggg
     5101 gcatactgcc ttcaagaaac aaaagcacca gaaggctacg cattacctaa gaatgaagct
     5161 gcgtacactg aatttacagt agatgatgat ccaacaactt ctgatgaaac aacgatcgat
     5221 aaccaaccta aaggaatctt gccttcaact ggtggtatgg ggatcatcgt attcatcttg
     5281 gtaggtactg ctttagttgg aggagcagtg atctacttca aaaaacgcca tcaagaaaca
     5341 gaagcttaaa ctattggaaa caaaaatcat acgataaata aacgatagtg attcaaatgg
     5401 ggagaaaggt tgtgacaagt ggtcggtctc ttggtattcg ctcaacaaac ggcaaaatag
     5461 gaggaactat tttgccgttt gttgagcgaa tactcaggtc aaggcgctga tttcacaacc
     5521 tcttcatata catattagaa aataggtgga catgatgaat aaaagcagaa taagaatact
     5581 ggacatcttc atggcaatca ttttagtcgt tgggatcggg atcttttctt atccttttgt
     5641 agaagattca ctgaatgatt ttcttgccca gcaaatgatc atccattatc aaaagcaagc
     5701 atcgaataag aattcagagg aaataaaaaa acagcaggaa aaaatgacaa agaaaaacca
     5761 acagttggca gagcaaaacg tatcaccagg gataggcagt ttcaaccaag cagttgatgc
     5821 aaaagcatta aaagatttac ctagtaatgc cttttttatg gaacatatgc ttggagtcat
     5881 cgaaatccca aaaatcaatg tcagcttacc tatattcgat cagacaacgg aaatattttt
     5941 gcaaaaagga acgtcattat tggaagaaag ttcttatccg actggcggga aaaacaccca
     6001 tgccgttctt tctgggcatc gtggactccc agaagcaaaa ttatttacag atttgcctaa
     6061 gttgaaaaaa ggagaccagt ttttcatcca gatcaatgga aagacattgg cgtatcaagt
     6121 agaaaaaatc caagtggtct tacctgatga agtagattct ttgggtatcc aaaaaggcag
     6181 agatctcgtg actttgctta cctgtactcc ttatatggtc aatacacatc gactgcttgt
     6241 gacggggcat cgtattcctt atcaagcaaa agaggcaaaa aaagcgatcc aaggaattga
     6301 tcaatggaaa aaatggaaat tttttatttg gtttatcggt atactacttg gatcaatcgg
     6361 cttagtttgg ctactcatcg cttatttaga ttctttggcc atcgctaagc gaaactatcc
     6421 tttatctttt tatgtaaaga acacaaacgg acgaccaatc gaaggaatgg tcttttctgt
     6481 aaaaacgctc aacggcaaac actatattac tagagaaaaa gttccatttg tgaaagcttc
     6541 tgatgaatac ggactagtga gattttctga tttaaaaggc aggaactatc gcttgcaaca
     6601 tgaggagctc ctgcttaaaa tccatgtaaa acataaacat tcgaaacagt tttcaatgaa
     6661 attgaaaaaa ggaagatata aactgcgaaa agaaaaagag gtgtactatt tgattgagaa
     6721 agaatgaatg aagatgtcca aaaaagcaga gggaacagac taaatgaaaa aagagagaag
     6781 ctgacgaaat ggacgtgtaa actgctattt ttggtttact atctggaacg ccagcttcta
     6841 tttttttatg tttcagaaga attttattat tatagtataa cttttgaatt agtttccata
     6901 acatttggag caaggggtta aaccacgagc ttgagcttcg cttaaagtgg caggggtata
     6961 cgtaccattt ccacatttat gtgtatggta ttttgaaccg gtcggtgtga catagacagt
     7021 ttgttcatta ttttgctcag gagctgcggc ttgtgcctgc tgagaggcct gttcagctgc
     7081 tgcttgctgt cgcgcctgtt ctgcttgttg ctgtgcgatt gcttcttgtt gcgcttgttc
     7141 tgcttgttgt cgtgcaattt cttcttgctt tttccgttct tcttctgctt cttttgcttt
     7201 gattgtggaa tcaattgcag caagacggct tgtcaactct tgtttgtttt caggtagcgc
     7261 ttggatagct gctgaagcag ttgcatagtt ttcttttgta ggatttgctt cagcttgtga
     7321 caaagcagat tccgcttctg tcactttttt ttctagtgca gctttttccg ctttttgttt
     7381 ctcttcttct tttttctttt caatttgttc ttgttctttc gatgaatctg ccgtactttc
     7441 ggagatcgtg gtactgctgc tggaagcctg agctgaactt gtgctttgag tagtagacgt
     7501 agtagcacca cacccactca acaaacttcc tgcgaccaaa aacaatgcaa ctaatttttt
     7561 cactaaattt cctccttaag taaaaaacaa ccgttttcaa taaatgtcct tttcaattgt
     7621 ttacttactt gttataataa ttgtgttatg aatagtgatt gaggaaactt aatctaatcc
     7681 cgttttgata cacgggattt tttgctatag ttctgttatt aagtatgcaa taataaaata
     7741 tgtacgttat tttattatta gattattgac atttatataa taaacgatta aatgattcat
     7801 atcaatagaa tacttaggaa aaaaagagaa ataataacag gtttatgcac tattgacggt
     7861 gtgacaatat gatatttgta tccgtttttt tcttgtatgt agtatagtaa agtagataca
     7921 ggaggagaaa atatgcagac ttttttaaca aaagtttctc aaaagaaaat taaaatgatt
     7981 catttactgc ttgaaacaga acgttggtgt tcaatagaag aactacaaaa cgaattgaag
     8041 gtatcaagta agtctatctt gaattatcta acagatttag aagaattgtt ccaaaaatat
     8101 cctgataagg tcattttgaa gaatgagaat aatcggcgct tttctatgga aaagcaggag
     8161 aacttcccaa tctacattat ttatcttcat ttctacagaa aatcatataa ttatcatttg
     8221 attgaattca tgtatcaaca tcctgagaaa aatttggaag attatgcaga cgctcaatat
     8281 acgagtgtat ccacagtttt tcgttatgct aagttattag taaagtactt tgaaatgacg
     8341 gacgtcaaag aatagtagcc gccagataga aggaaattag cagttgaaaa agctgccctg
     8401 catcaagtaa tacgaccagc caaacggaaa cagtgtctca tgtttttgat ttggctggtc
     8461 ttattaccgc agatgcaagc ttttgccaca cctctttctt gtcatttaca gactcaacta
     8521 tcaatgctgc aagctttccc ttaatgctgt aacaccagta agagttgtca agaccagcag
     8581 actgactagg taaataccgc tcaaaaacaa gattacggca gttaaactat aatgatccat
     8641 caagaaaccg atgacgatag aagaaaaacc accaacagct cgtccaaggt tcataatgaa
     8701 attattcgcc gtcgcgcgga tttctgttgg atacaaacta ctaatcactg cgccatatcc
     8761 accatacatg ccattgataa agtagccgac tatcacagcg gctgctacca aactccactg
     8821 gctatgggct aacacaagta gatagacaga taaggatgaa caaattaaaa acaaagaaaa
     8881 ggctagttta ggccccaatt tatccatgat tatgccaaaa gtcatcatcc ccaaagacat
     8941 gcctaaaata gtactgacca tccacaatga agacccagaa accgaaagcc ctaatcgatt
     9001 ttgcataatt gaaggcagcc aattcattaa cccaaaatag ccggcaattt ggacagttac
     9061 catgatacta aggccacatg tttgccaagc aagatgtttt gattcaaaca gtctgctaag
     9121 tttggttttc ttttcggcag attgtacaga tttgaattcc tttggttcag tcaagccgcg
     9181 acgaatgaac aagaccatca acaccggcag tacacctacg acatacaaca tcttccaacc
     9241 aaacgttgga atgatcacag cagctaaaat cgcagccaaa gccgcaccga cctgcgcacc
     9301 aataccagca accgaagaag ctcgtccaat ttgttttttt gaaaaacttt cggaaatcaa
     9361 ggacatacac gccccgtatt ctcctcccgc tcctacacct gcgataaagc gaaagagata
     9421 aaccaagtag atgtttgaag caaaatacat caataaagaa gcaatcgaaa acagaaggac
     9481 cgtttgagaa aatactttca cacgtccata tttatcagct atgatgccaa aaaaaatacc
     9541 tccaatgagc atccccaaat tagtgatcgt tgaaatgaaa cccgcttgcg taccactaat
     9601 atgaaaagaa gtaatcattg aagataatga aaaagctaaa aacataatat ccatattctc
     9661 taaagcaatt cccgccgatg tcgataataa taactttcgc tgatacgttg tcatttttat
     9721 accccttttc ctaaaatttc cctaaattgt taaaagtata tccaaaaaag aataaaaaaa
     9781 caagactttt ttatttgaaa taatacacaa acaaccgata tagataatcc agcttagttt
     9841 taatagaaat gagtattgac ttggagtaca cttaaagttg tagaataaat aaaatacata
     9901 agtaaagtgg caagaaatta agagaaagag tgatcagatg aatatcaaac aagtcagtga
     9961 agaaaagggc atttcggccg atacattgcg ctattatgaa cgaatcgggt tgattccacc
    10021 agttaatcgg acaaaaggcg gaatccgtga ttatacagaa gaagacctaa aatgggtcga
    10081 ctttacgatc tgtatgcgaa atgccggatt atcgatcgag tctttgattg aatatattcg
    10141 cctatctagc gaaggagaag caactgtatt tgagcggcgt aagcttttga ttgaagaaag
    10201 tgaacttttg acgaaaaaaa ttgctgaaat gcaagaatgt ttggaacgtt tacaaggaaa
    10261 aatcaagaaa tatgatcgtg tgttatctag aaataaaatg gaatgtatca agtaaaaaaa
    10321 tcaaggagca tcgctatgga gttacgctta ctaaaatatt tttggacaat tgcagaagaa
    10381 ggaacaatct ctcaagccgc agaagttcta catcttacac aaccaacctt aagccgacaa
    10441 ctgaaggagt tagaagagga actaggaaca gaactgtttg tacgcgaaaa ccgcaaaatg
    10501 atcttgacag aagcaggtta ttttctaaaa aagccgagca gaagaaattt tagatttaac
    10561 gaatcaaacc actcaagaat tcgagaacag aaaaaaacaa ttattcagcg gccgatttac
    10621 gatcggctgt gtggaagctg ataattcgaa tacattggca ctattactag aaaagttgat
    10681 ccgtgattat cccagcgttc aatttcatat ccacagcgga acaagtgatg atatcacgga
    10741 gtgactagaa aaaagcctgt tagatttagc gattttgcta gatccagtag atacagagaa
    10801 atacgaaacg attttgttac caagaaagga aaaatgggga ttgctcattt tagaaaattc
    10861 tgttttagca aaaaaagacg tgataacaaa agaagacttt gcgaatgtac ctcttttgat
    10921 ctcaaagcgt ccagaaatac agcgaatgtt tgctgactgg ttaggcataa aaaaggagca
    10981 gttgctgatc acaggaacgt acaatttgat tttcaatgta tttgcgctaa ttgagaatca
    11041 ggtaggtgct gctttagtca tagaaggtgc tgtaagtcat ctttcaaagc gagtgacatt
    11101 cgttccgctt gacccaatga ttgaaacgaa ctgcatgcta gtctggaaaa agaatcggat
    11161 tttatcacct gtagtcaatg aattgattca ccgtttcaaa gaagcttttg aacagtaata
    11221 agtatctcta atcgaagaaa tttcaataca aaagcgagac gtatgacagt cttgcttttg
    11281 tattttttca tttttaaatc aaaaaaaata aagagattta cataacttgt taaataagcg
    11341 cttacaaaca taagaagaac ggaaatgaat cagctataat aaccgttgtt ctggattgta
    11401 atcattacaa agaaatgaag attataactt tggaggtgtt cgaaatgaaa gaaaaaaagc
    11461 aaaaattcgt tcaaaaaaca tgccctttat tcaatggttg ggtatgattc tgctgattgt
    11521 attagcttta ttgacgatca ttcctttttt atggatggtt tctgcttcat tcaaagcaaa
    11581 taatgaagtg ttcacgattc cgattcaatg gatccctaaa acttggcatt tagagaatta
    11641 tctggttatt tggtctcgta ttcctttagt gacatttttt aagaatacag cttttttaag
    11701 cgttgtgatc acgttgattc agttgtttac ttctagtttt gctgcttacg ggttttctaa
    11761 actaaatttt aaagggcgcg atattttatt tttagcatat atcggcacga ttgctgtccc
    11821 ttggcagtct tatatgatcc cgcagttcat tatgatgaga aaagtgggtt taacatatac
    11881 gttgtggtcg ttggtgctat tacaagcgtt caatgctttt ggtgtgttct tgttgaagca
    11941 atactacagt agtatcccag attctttatg tgagagtgcg cggatcgatg gcttatcaga
    12001 gtggggaatc tatcgcaaga tcattttacc tcttacaaaa ccggcgattg ccagtttgac
    12061 gatcatcaca ttcgttaata catggaacga ttatatgggg ccattcattt atttgtcttc
    12121 tacggaaaat aaaacaatcc agttagggtt gaaaatgttt attgggctat ttgatgcaga
    12181 gtacgcatta atcatggctg cctcagttgt ttctgttctt cctgttgctt ttatattttt
    12241 agcgatgcaa aaatattttg tagaagggat cgcttcatca ggaatgaagg aataggaggt
    12301 gattatgaaa gagcagcgtt ttgacaaaaa tgtttatatg gaattctttt attggttgta
    12361 tcaatcattg gcaagtagcc tttgtttttg gtgtgtcaat atcttgtata tagcagctgc
    12421 gttccttttg tcgatagatg gaaggaatgt cttttccttt attttttctt tactttttat
    12481 tggtccaggt tgcatcgtga tgataggtat gacagaagaa ttagcaaaag aaaaaagttt
    12541 ttcaccagta cgtttgttca taaagacgtt tcgccaattt tggctaaagg gattttttta
    12601 ttggctattt gcttggatag ttagtgtgat aatgattttt gattgtttct tttttatacg
    12661 tttttcttat ggaaaatggt tgattccgtt gtttgtttta cttgcttgtt tatcagtttc
    12721 ctttagcatc aactactggt attttcaggt gagaaatcca gcgtcgaaac caaatcaagt
    12781 tctacggata gcgttctact atacgttgaa aaaatggtat gtgagtttac tagatttttt
    12841 actattgaca agtttgtttc tcttcttttt cgtaaagccc caatggtgta ttctactagg
    12901 tccttctatc gtatttggtt tgatttactt caacaatcgt aaattgatga gaactatgga
    12961 tctgtagact ttataaattg aaactttcaa ttaagaatga cagtttttat gtaaaaactg
    13021 tcattctttt tcattttttg aaacatatgg ctaaacaaat gaaataaaac gtgctatact
    13081 tttgaatgaa acaggtttag aaactgtatc aattatttgt gattatttct aactaataat
    13141 taaaaataaa gcataaaaag gacgatgttc aatggacgaa aaaatgaaac aaggttcgaa
    13201 atctgtagta gaaagtttga ttaatcatca tgttgattat gtattcggta taccaggtgc
    13261 aaaaatcgat ggtgtattta atgaattaga ggatcaaggt cctgaattaa tcgtgactcg
    13321 tcacgaacaa aatgctgcat ttatggcaca agcaatcgga cgtattacag gagagccagg
    13381 agtagttatc gcaacaagtg gtcctggtgc tagtaactta gctacaggtt tggtaacagc
    13441 gacagcagaa ggtgacccag ttttagcaat tgccggacaa gtaaaacgaa gcgatctatt
    13501 gaagttgacc catcaaagca tggataatgc agcactgttc cagccaatca caaaatacag
    13561 tgcagaaatc caagatccag aaacaatttc agaagtgatt gccaatgctt atcgtatggc
    13621 taagagttca aaaaaaggcg cgagctttat cagtatcccg caagatgtag tggatgcacc
    13681 tgtccaaggg aatgtcatca agccattgag cgatcctaaa ttaggttctg cttctgcaga
    13741 tgatatccgt tacctagctg aacgcattag agaagcaaaa ctaccggtac tattagtagg
    13801 tatgcgagga tcaagtgaaa aagaaacgtt agccatcagg caattagttg gaaagacagc
    13861 attgcctgtt gtagagacat tccaagcagc tggagtgatc tcacgtgaat tagaagcgca
    13921 tttctttggt cgtgtgggtt tatttagaaa ccaaccagga gatatgcttt tgaaacgaag
    13981 tgatctagtt att
//
LOCUS       DACEXQ010000046        13951 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.046, whole genome shotgun sequence.
ACCESSION   DACEXQ010000046 DACEXQ010000000
VERSION     DACEXQ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13951
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.046"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            116..718
                     /locus_tag="IXH04_001037"
     CDS             116..718
                     /locus_tag="IXH04_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ1851661.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(806..2305)
                     /locus_tag="IXH04_001038"
     CDS             complement(806..2305)
                     /locus_tag="IXH04_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAQ1851662.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(2321..2845)
                     /locus_tag="IXH04_001039"
     CDS             complement(2321..2845)
                     /locus_tag="IXH04_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851663.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            3143..3892
                     /locus_tag="IXH04_001040"
     CDS             3143..3892
                     /locus_tag="IXH04_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAQ1851664.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            3883..4668
                     /locus_tag="IXH04_001041"
     CDS             3883..4668
                     /locus_tag="IXH04_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ1851665.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            4637..5353
                     /locus_tag="IXH04_001042"
     CDS             4637..5353
                     /locus_tag="IXH04_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ1851666.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(5455..6474)
                     /locus_tag="IXH04_001043"
     CDS             complement(5455..6474)
                     /locus_tag="IXH04_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAQ1851667.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(6493..7551)
                     /locus_tag="IXH04_001044"
     CDS             complement(6493..7551)
                     /locus_tag="IXH04_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAQ1851668.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            complement(7772..8920)
                     /gene="nagA"
                     /locus_tag="IXH04_001045"
     CDS             complement(7772..8920)
                     /gene="nagA"
                     /locus_tag="IXH04_001045"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAQ1851669.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(8996..9832)
                     /locus_tag="IXH04_001046"
     CDS             complement(8996..9832)
                     /locus_tag="IXH04_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1851670.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(10016..11233)
                     /locus_tag="IXH04_001047"
     CDS             complement(10016..11233)
                     /locus_tag="IXH04_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ1851671.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            11616..11699
                     /locus_tag="IXH04_001048"
     tRNA            11616..11699
                     /locus_tag="IXH04_001048"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11650..11652,aa:Leu,seq:gag)
     gene            complement(11939..12562)
                     /locus_tag="IXH04_001049"
     CDS             complement(11939..12562)
                     /locus_tag="IXH04_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851672.1"
                     /translation="MKFTNIKKYRAFFLDMNILFLLLFFLIFLLTYRKCQNYYVRILT
                     ALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIA
                     TLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYI
                     LYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
     gene            12652..13947
                     /locus_tag="IXH04_001050"
     CDS             12652..13947
                     /locus_tag="IXH04_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ1851673.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
BASE COUNT         4245 a         2836 c         2409 g         4461 t
ORIGIN      
        1 tcctatacgc atttacttcc ccctttttta ttttaattat acttgaataa tgaaaaacta
       61 gctatcagac aaaagtttag gtatgatagt aaaaagacca aaaaggagaa tctctatgca
      121 atttggccgt tttcgactag gtatgcgtac gatcaaaagt gccttagctg tttttttatg
      181 tatcttgttt tttcatttga ctcaacgagg aatcccgatg attgctgcat tatctgctgt
      241 tttttctttg cgtcaggact tgactacaac cgtttctttt ggaaagtcta ggatcatcgg
      301 caatacatta ggtggtacat tagctatcat ttattttttt gtcaaaagct attttcacaa
      361 tgattttttg gtagaattga tcttgcttcc tattctagtt attattgtga tcgtcgtgtc
      421 agatggtatt aacaataatt ccggtatcat ttccgcaata gctacgttgt tgctgattgc
      481 gttgagcatc cctcaaggcg aatccgcttt gtatgctatc caacgtgtac tagatacttt
      541 tatagggaca ttcattgcaa tcggtatcaa tttctttctt cgtcctcctg aaacagaaaa
      601 gaaggaagaa ctatcagaag atttggtcga actccaaaag aaagaacgat tgctaagaac
      661 aaatttacaa gagatagaag ctaagatcca aaaacaaaag aaaaatagcg aggactaatt
      721 ttcttttgca ataaaataaa aaaatggaga gaagaacttt aaaaagtctt cctccatttt
      781 tgattgaacg gtgaagtgta ctactttatt cttccttcat cgagttttgt tctgcctctt
      841 ttggcgcttc atacatattt acttggctag tagtcccacg tgtcgacaaa ttctctacga
      901 tttccttcaa gtcaagtcct gttgtcgctt ttagtgtttc ttgagtagaa gaaagcaaat
      961 ttgtggcata atttgtcact cggttcgctc cactggtttc tcctgaactg cttcctgtat
     1021 cgacaacaga gattttttcg atatttccta gcggctgtgc tgcttcgcgc atcaattgcg
     1081 gtagcatatc gataaccata ctcaagacag ctgcttcgcc atattccttg aaggcattgg
     1141 cgatcttttg ttttgcttcg gcttctgctt ttccttttgc tagtgctgct tcggcttctg
     1201 cttgtccagc taaacgtgtc ttattggctt ccgcttctgc cagtgcttcc acacggaatc
     1261 gttctgcttc ggcttcagtc acttctttga ctttttgagc ttgtgcttct tgttctttag
     1321 cgtaccgatc ggcatctgct tttttcttca cttccgaatc atattgcttt tcacgacgtg
     1381 tgatttcttt ttcttccagt tcgatttgtt tttgacgttc tacgatttta atctgcattt
     1441 cctgttcgat tacttgttgt tgtgctcggg cactttctaa attgtaggct tggtctgctt
     1501 ttgctttagc aacatcttgt tcttgtttgt aggttgctaa tttcagttct ttttctttta
     1561 atgattctgc gatctctgtt tgtcgctgta attcagctgc ttgtgattct ttttctgctt
     1621 gagctttttt gatacgcgtt tcttttagag cttccgcttc tgctatatct gcatctcttt
     1681 tgacttgggc gattcttggt ttccctaaag agtcaagata accgtttttg tctctgacct
     1741 ctttgatagt gaaagaaacg atgatcaacc ccatttttgc aaggtctaca gaagcgactt
     1801 cttgtacgct ttgactgaac ttatcgcgat tttgatagat ttcttctaca gtcatagaac
     1861 caagaatcga gcgcaagtgt ccttctaata cttctcttgc ttcattttcc agttcttctc
     1921 ttgtcttgcc taaaaactgt tctgctgctg tagcaatctc ttctacagat gaaccgattt
     1981 tgatgatcga agtaccatca cacatcaccg gtacgccttg ttccgtatac acttcagggg
     2041 tggagacgtc caatttactc gataatagac tgatccgatt cgaacgttga aaaactggca
     2101 agacgaatgc gccacctcca cgcacaattt tgattttgtt attgctttca tcaatgtgca
     2161 catttttgct tcctaaataa cttccactga tgatcaacgc ttcatccggt tttgctgtct
     2221 ggtacttcgc tacaaagacg atcagcaaca tcagtaaaat caaagctgcg attgcgatcg
     2281 cgataaaaac aactggtatt tccatttaac acacgctcct ttaagctttt atcatgcttt
     2341 cataaggcat gacataacag attctttcct tgatctcaat gatcaatact ttcgttcccc
     2401 gctcagctgt ctgttcttgg gggctgtaaa aacaagctgg acgactaatg gaaccggtaa
     2461 cacttttcaa ctgaatttct cccatacctc gtacagggat cggtgtgatc acaacagcta
     2521 cttgtccttc cagagttttt tcagaagacg acatcgttga ttcggcatgt ttcatcggca
     2581 tcaatatata gaaattaagc aaaaatacca acactatcga tatgcctcca ctagcaataa
     2641 ataaaaataa agatgattga tctgttaaac gctcacctag aaaaccaaat aagcttgtga
     2701 aagttaacca agggataata agcatcggat caagcgggcc atcaaaatcg aatacatctc
     2761 caaaaataat caataggact gccaatcctg cacaaatcag caatgtatac caataaatcg
     2821 tctcaagtgc gattccccct atcatataca cgcctccttc catttctacg tctatcatac
     2881 caatgtttga aaattctgta tagcttcttt taccgactca gtggtgtttt ttataggaag
     2941 gttgtgttta tcgttgacaa tttgttaaat ttttgttttc atgttaaaat tttcacaata
     3001 aattctttcg aggtagccta tgtttccttt ttttaagaaa aaaccattaa agctccagaa
     3061 cacgatcatc cttgtcgttt gtttctccac attcgtttct ttgattgttt ctgccatatt
     3121 gatcaggaat tttattcttt cgatgaaccg atctagtgtg tcagataaaa tctctagcat
     3181 tgctaaaatc gttgccaatg ataaactcat ccaaaaaaag ctgcagactg gtgacccgac
     3241 acatgcgatt caaggaataa caaatgaaat catgattgca tcgaatttag attttatcgt
     3301 tgtcatggac cctgaaggca ttcgatattc tcacgcagac ccaagttaca tcggtcttcc
     3361 tttctcaagt catgaagatg tgaaaaaagt gattggagga caagaatttt tctctgaaaa
     3421 agaaggaatg ttagggttcg gcttccgcta tttcgtccct atttattatg atcaagagat
     3481 tattggtgca gtttgtgttg gcttaacaaa caacacgatt gacaatcaag tcaaagatgg
     3541 acaaaaaatg attgtttttt cattgattat tggacttagc gtaggcatca taggcgccgt
     3601 ttttcttgcc caaaaaatga aaaaagtact gcttggactt gaaccacaag aaatagcaga
     3661 acagttaagt gaaaaaacga ttatcgaaaa tgaagtagat gaaggaatct tagccatctc
     3721 ttctgataaa actatcctct tgatcaatac acctgcaaaa aacattttct accaaacaga
     3781 gcagctattg gatttcgaag taggaaaaaa atcagtgata atctatatga cgttttgttt
     3841 aaagagacga ttgagcatca aacgaccgtt cggactcaat ccatgtattt aaatgggata
     3901 gaaactattt ctagcgttac acctcttttt agcaatgacc gttttttagg agcagtcgca
     3961 actttacgaa atcgatcaga agtaatgaaa ttaatggagg agcttggggg gacgaaacaa
     4021 tatataaact ccatgagagc tcaaacccat gaattcatga ataaacttca tgtcatttct
     4081 ggtttgattg atttgaagaa gtatgaagaa gtcacccatt ttatccagca gctaaatcaa
     4141 aattataaaa aagagattgg ccaagtctca agtctgatca aagtacctgc aattgccggt
     4201 tttttattag gcaaaataaa cgaagccaaa gaacaatctg ttgaaatgct gttagaaagc
     4261 gattctttta tccctaaaat ggagatggat gactccatcc attcactttt gcaagtgtta
     4321 gggaacttac ttgataatgc aaaagaagcc gtgctacaaa aacatgaaag aaacggacag
     4381 gtccggattc atttaaacta tgacacagaa gctgaagtat tcatcgtgac cgtctgcgat
     4441 gacggtgtcg ggatatcaga agaaatctca gaaaaaatat ttacgctagg tttttcgacg
     4501 aagggaatca atcgtgggtt tggattgaat ctcatccatt caatcgtcaa acgccacggt
     4561 ggatttttag aattttccaa acaaacaaat ggcggtacaa tcgtctatct agaattacca
     4621 aaaaaggaag atcaaaatga tgacaaatgt cctgataatc gaagatgatc caatggttgc
     4681 agaattgaat cgtacttttg tggaaagcat ccctaatatg aacgtaatcg aaagtttcag
     4741 aaatgtagaa gaaggaaaaa aatatttcag acaaaaaaac gcagatctgc tgctgcttga
     4801 tgtatacatg cctggcgaaa gtggtatcca ttttttatct tggcttagac agcaaggaaa
     4861 tgatatccca gtgattttga tcactgctgc agatgatatc gaaaccataa agaatgtgat
     4921 ccgctacggt gccttagatt atttgatcaa accgttcact ttgcaacgtt ttcagctggc
     4981 gatcgaaaag tatcttgctt tctctcagac aatccaaacg actgaaaaag ccgatcagaa
     5041 attgatcgat caagtattca accacaccac tcccattcac gaaataccat cattcatcga
     5101 tttgcctaaa ggtctgtcaa aactgacttt aaaaaaaata agtgcattta ttctaaaaga
     5161 agatactatt ttttcaacaa aagaactggc tcaaaaaagc tcactgtcac gtatatccgt
     5221 caagaaatat ttgaattttt tagtagaaca agatatattg aacgaaagaa tcaaatattt
     5281 ggaaatcggc cggccgctaa cctgttatga attgacaaag aatgggcgga aaaagctcac
     5341 agattactgt tgagtcatcg aattatccca tacaaaatag tccaagtata cagaagtaac
     5401 aactgtttac ttggactatt tttaaacatt ttattgacga atatccatgt tttcttatct
     5461 taattgacgg gctttaggaa gagatacctc atttttcgtt tttccagttt ctattatttc
     5521 acgaaagttc tcgaaacttg tcgaaatcat atttgataat gcctcatctg tatttgaacc
     5581 tacatgcggg gttacaagaa ctctagggta catttctact aattgctgga tagcagggtc
     5641 tggaatcgtc tcccatggct tgaaggatcg gaaaaaaacc tctgtttcat ttgcaaaaac
     5701 atcggttgca aaaccagcta aatgattgct tttcaaagct cttaaaatag cttcattatc
     5761 ttgaagctct ccacgggctg tattaatcaa gatgctacct tttttcattt gagctaaaaa
     5821 tccttcattg atcatttgat cgttttcacc tgggatatga ggtacatgaa tactgacaat
     5881 atcgcttttg gctaataact cttcaagcga gtggaacgtc aatacttttt tagcagcttc
     5941 tgtctgatga atatcgtagc cgatcacttt ggctcctagt cccttaaaca aaacagcttc
     6001 cgtcaaccct atcctgcctg ttcctatgat ccctactgtg caatttctga tttcacgact
     6061 aaacatttgc tcatccactc gaaaatcttt gaaagatgtt cggatcgtcg tatacgcagt
     6121 atttctcaaa agcatcatgg caaaagtcaa agataactct gctatcgcat ttggcgaata
     6181 cgatggtaca cgggctactg tcataccaaa atcagctgcc gcttgtagat cgatatgatt
     6241 gactcccaca gtccgtgtaa aaacataacg aatcccatat tcctgcattt ttgcaagatt
     6301 ggtctcatct gctatacaat ttcccctcaa caatacagca tcatggtcct ttgctgacac
     6361 gatattgtca tgtgtcaaca gttcttcata aagactgaga tcataatggt aaatattcaa
     6421 tttttcaaaa aaatctatct cgttcggacg aacaccgtaa catgcgattc tcatactttc
     6481 ttcctccatt tcttatgaaa ataaacctat gccatgaatc atttgtaata caacgatcca
     6541 tacaggtgtc agtaccacag caaaaatagt tgacagtaag gatgcattgg aggctaaaag
     6601 tgcctcccta tcaaaactga tagcataggc tgctgccact gttgcagctg gtgtagccat
     6661 catgataacg attgttgcca gtgcttcaaa attgactggt agaatattcg ttgccgtcaa
     6721 aactgctaga atcacgatat tcaatgcagg tacaaatacc actttgacta gactgtaata
     6781 ccatgaagtt ttttcagatg cagctttttt gaagcttact tcacctaatg ttgcgccgat
     6841 tgctagccac gccaatggtg aacttaaacc agctaaataa tccatcggct gaaatagcca
     6901 cggtgcagtc tggtcaattc gcaaaaacgc tacttggctt atctctcctt ctgcacctgt
     6961 aactgacact tgtggcagat acccttggaa cagccaaatg aaaaggccgg caaaagtagc
     7021 gataatgatt ggattgacga acatcgcttt gatattttta gcttccatct tcaagccact
     7081 catttttatg tatccataag aatacaaaaa gattcggtag ccaatattga aaattgagga
     7141 atacatcacg ccgacaggac cataaatcgc actgacgata ggcgttccga aaaaggtcgt
     7201 agaaccaaag atcgtcagga cacgcaacgt tgtttgctta tcccctttat agcttaagaa
     7261 aaaaggtttt gaaactagaa taagaataat ataaataact aatccccaaa tcaagacatt
     7321 gattccttgc atcagtaatt ctgtattgat atcttgcatg aaagctcgaa atgccagtgc
     7381 tggcaaagcc actgttaaga cgactttcga tagaattttt ccaacagatt cagaaaaaat
     7441 cccctttttt ctacaaacaa accctaataa tataataaaa actgttgaag tgattgcact
     7501 gacaatcgac atatctgtca aagtcgatcg aaaaatgtca aataagttca tctttctcct
     7561 cctaattatg ttcatttatt cacaataaaa aaagctttta ttttgctaat acgtattaac
     7621 atttttaatt ttatctttac attgttactt attgaacaat aggaaattaa agaaactttt
     7681 gaaacttatg taaagtgatt tttactagaa cacaaaaaaa accttaaaac caaacttaag
     7741 aagtttagtt ttaaggttaa cttaaattat attatgcgtg gaaacgttcc acgccatcta
     7801 gataagttgc ttccaatgtc atatcagggt tcaatacgat aaagtctgca tcgcgtcctt
     7861 gagcgatttt tccacatgta tcatcgattt tacagctgat tgctggaatc agactagcca
     7921 tcatgactgc ttgttctggt gttgctaatc cccagtctac aacattttta attgcttctt
     7981 tcaatttcaa tatacttcca gctaagttgc cagattctag acgtgctgtt ccttctttta
     8041 ctactactgg aaattcgcct aaattgtaat ttccatcagg catcccaccg gccatcatac
     8101 agtctgtaat taatgcgaca tgatcatgtc ctgctttttc cattaggatt cccgcagcat
     8161 ttggatgtac atgatggcca tcacaaatca attcagaaaa gacatgttct aaagataata
     8221 acgcacccac catacctggt tcgcgatgat tcaatccacg cataccattg aaagcatgga
     8281 caaatacact tgcgcctgct tcaacagctt tttgcgcttc ttctagtgta gcattactgt
     8341 gtcctagaga aacaactact ccttcttcag tcacttgttt gacaaactct tctacacctt
     8401 gacgttccgg tgctaaagca atttttttga ttaaaccacc tgaagcttct tgccattcat
     8461 ggaaagtatc caaatcagga tccccgaaat aacttgggtt ttgtgcccct ttgtgttctt
     8521 ctgtgaagaa aggaccttca aaatagattc ctttgatttt agctcctggt gcttcctgat
     8581 acattttgcc gattgtctca gctacatctt ttaaacgttc tttgcttgac gtcaatgttg
     8641 ttggtaaaaa tgaagtcact ccacaagata acagaccttc agacatcact ttgatgcctt
     8701 ctgcatcatt atccattaca tcatgattca tatagccgtg aatatgtgta tctaccaaac
     8761 caggagcaat ccatttgccg ctttggtcaa tgatgttgac ttctccctca ggtagttctt
     8821 tggtgtattc accaaaaagt ccatcggtaa tttcaagata gcctgcatgt ttgacacctg
     8881 acgctaaaaa gaatttgtca gcatagataa aagttctcat tctattctct ccctttgttt
     8941 ttctacctct aaagtactct tatttagaga agaagtcaag aatggtctac accatttaca
     9001 aattttttga aatttgtttc acagcttgtg ctaagccttc atagaatgct gctctttctg
     9061 gttcttcttt acgcttgtat attaagcttt taagcacaaa taaatcattg aggtaatagt
     9121 agctattctt ctcttgagcc caatagattc cttgattcac ctgttctaac gcttcgtcga
     9181 tttcattttg atggaataag aaatttccat aattataaaa aatttgcgaa aattcacttt
     9241 ttgatcgttc atcagctgat tgatgcagca gttggatgct tcgactcaaa tagtagcgtg
     9301 cttcagccgt ttttcctgcc acaccatata tttttccgat acaactaatc agttgtattt
     9361 ctgtgtcaga aacatggatt ctttcttttt ggctagagta gcttaatgct tctttcaaat
     9421 aataaaggga ctgagagata ttttgacgca acgtgaattc gcaaacaccg agataataat
     9481 aatagcgttg aaagtcagtt gcttgataca gattttcaga aatatcagaa gattttagta
     9541 cttgttctaa cttccgataa tcacgttttt ggaaacaact atccaacagt tcaaataatt
     9601 tatcgtttgt ccgagtagtt tttcgagaga gattcatcac atgatcaatc gttaccccta
     9661 gccgttgaca aatctgcatc atcaccaata cattcggcag ttcctcatta ttttcgatcc
     9721 gacttaatac actttgtgaa caaatatcat gcgaaagcat tttttgcgtc attttttgtt
     9781 cttttcgaat ctctttaatg atcgttccaa aggactcgat taacgtttcc ataagtatcc
     9841 tccccaaaat atgcgtattc gcattctatt cgtattttac tttaggaagt cgcaaacttc
     9901 aagaaaagac ttgcaactaa ataatatact ttagttattt aagaaaactc cactagaaag
     9961 agagcctcag tgtgtttgtc tgtggccctc ctactgaaaa atttggtttt taaaatcatt
    10021 tttggcgata ggttgctagg aattctctca ttttcttagc tgtttgtttc aaatcatcca
    10081 ttttaggaag ataaacgatt cgaaaatgat ctggctggtt ccaattaaat ccaccaccat
    10141 gtacaagtag aatatgatgt tcatgtaaaa agtccaaaac gaatttttca tcatccaaaa
    10201 tattgaatcg ctttgtatca atttttggga atatataaaa agcagctttt ggttttacgg
    10261 ctgaaagacc aggaatatca ttgattgcat tatagatata ttctctttgt tcatagatcc
    10321 ttccgcctgg caggagcagc tcatcgacac tttgataacc gcctagcgcg gtttggataa
    10381 tttgctgaga caatacattg gaacacaaac gcatggagga caacatattc agcccttcta
    10441 tatagccttt gacgtgtgac ttatctccac ttaggaccat ccagcctact cgaaatcctg
    10501 ctacacgatg agatttggat agaccgttca gcgtaacaac gaacaaatca ggcgctaatg
    10561 tagcaatcgg aatatgttct agaccatcca tcactaagcg atcatagatt tcgtcggaat
    10621 aaataatcag gttattttgt cgagctactt caacgatttc ttctaataat tcttttggat
    10681 aaagtgcccc agtaggatta ttaggattaa tgataacaat agcttttgtc cgactcgtta
    10741 tttttgattt gatatcatca atatcaggat accattctgc ctcttcatcg caaatataat
    10801 gaacaggtgt gcctccagct aatgcaatag aagccgtcca caacggatag tccggcattg
    10861 gaaccagtac ttcatctcca ttgtccagca acccctgcat acacatcgtg atcaattcgc
    10921 tcactccgtt gccggtgtag atatcattga tcgtcacatt cggaaaatgt ttcagttggc
    10981 aatattgctc aattgctttt ctagcagaaa agatcccctt ggaatctgag tagccttctg
    11041 agtcacgggc attcatgatc aaatcacgaa tgacttcgtt tggtgcttca aatccaaaag
    11101 gtgcgggatt tcccgtgttc aattttaaaa tactaatgcc ctcttcttgc atccgctccg
    11161 cttcttctag aacgggaccg cgaacatcgt aactgacacc ttctaatttg ttggattttt
    11221 caaattctct catcacttag ccaatccctc atttcaaaaa gttagaactt tccaaatttt
    11281 ttgttattta ctatttcaat atcatactcc atcctagaaa attttgccac ttatttagga
    11341 aaaacgtaag tattaaaaaa acacaaacaa gcgttgagaa cttaattgtc atgatagtca
    11401 ttccatagat tcgctgccca atccattgca attccacaaa tgattcctag cataaaacca
    11461 agcaaaaaac gtccgaatgc aagtccaatc aatacaccta agatcattcc cagtcctgaa
    11521 aattctccgt catatttctc catcttaata agtcctccca tttttgagct tgacaactat
    11581 gtaaaacctc gctatcatta gtgtgttatt attacgcaga tgtgttggaa ttggcagaca
    11641 agcatgattg agggtcatgt gagcgttagc tcgtgtgggt tcgagtccca tcatctgcac
    11701 tacaactcgt gaagttaatt ttgcttcacg agtttttttg tttgaacgtt tctttatgcc
    11761 attatataca tatcactgta tcctttacaa aaagaacgta caatcatgta aacagcctga
    11821 ggtgagtata tgaaggtcgg aaacaagcta ctcgtagaaa cttgcaagtg ttaaaacaat
    11881 taatcgaaaa acgagtaaaa tccctcacct aatactaaag cgagggattt tttgtatatt
    11941 atctgtttat caccgttatt ataaaggtac aaataccaag cacggttaat ccaaattgca
    12001 taaattcatt tttttgtaca ctcatgcttt tcattagctc tttaactgcc attagtacta
    12061 agatggcgat aacaacataa agtatatagt acgtccgcat tccggaagcc catactgtgg
    12121 gagaaaatcc cataattaac cttgaacaaa atcccattat catcaaaata actaaaaaaa
    12181 tagaagtttt cttattgtca aaacttaaat aaattccaac aattaaacat agtaataatg
    12241 ccaagactac taaagtagct atccaagtac ctggataaaa aagtgacagt ttcgttccta
    12301 actctgtaaa gagattattc aggttactac tactccaaat cattgccctt ttatttccat
    12361 tgacgtaagt aaagctttga cccattgtat taccaaaata aataatcagg tttaagaaaa
    12421 acggtaatgc agtcaatata cgcacataat agttttgaca tttcctatat gttagaagga
    12481 aaattaagaa aaatagcaat aaaaataaga tattcatgtc taaaaaaaag gctctatact
    12541 ttttgatgtt ggtgaacttc accaacatct tttttgtata aaaaaatata gaaaagacac
    12601 cctgaaatcc tttagaatta atttggctac caattcatag gaggattcaa gatgtctcat
    12661 actcagctta tcaaagatac cctaaatatt ctagacctaa atatctattt cgaggaaaat
    12721 tgtttaacta aagaaaaata caaagggcaa atttgcttga tttacaaagg aactcttctg
    12781 tacaagccag aagagtgttc ccattgtctt tgtgtcgtcc cctctaggat cattcgttgg
    12841 gggacgacaa ccgttcggtt gttactgaat gatgtgtctg agtatcgcac ttatctggag
    12901 ctgaagaaac aacgatttaa atgtaagtct tgccaaagaa cattcgtagc ggatacctct
    12961 gttgctgaaa aacactgttt cattagtcaa aaagttcgtt ggtctgtcat tgctcgctta
    13021 aaagagaata cttccatgac ggaaatcgct cgtcagaaaa atatttccac ttcttcggtc
    13081 tatcgtgtca tgaagcgatt ctaccgccca ttgaatcctt tcaaacagac tttgccaaag
    13141 gtcttatgct ttgatgaatt caaatcggtt cgaagtgttg ccagtgcaat gagttttatc
    13201 atgatggacg gacaaagcca tgcattattg gatattgtcg aaaatcgacg cttgccttat
    13261 ttagaacgct atttttctcg gttttcatta gcgactcgtg aagccgttca attgattgtt
    13321 attgatatgt atgcgcctta tgtttcgttg gtgaaaaagc ttttcccgaa tgctcagttg
    13381 atcatcgatc gtttccacat cgtccaacat attggcagaa ctttcttgaa tcatcgagtg
    13441 aaagaaacca cggtacgact taaaggaaat tcccactctc aacgagggtt agggaaaaaa
    13501 ttgaagcgct attggaaact cctacaaaaa gaagaagcaa agttaaacta tgaaaaacgg
    13561 gtttggcgtg ccagttttaa agattatttg actgaaagtg agattgccga tcgactgtta
    13621 gctgcttgtc ccaacttacg ccaaggctat cagttatacc aagacattct ttatgcggta
    13681 aagaaaagag atcagtcact atttgaggat tgtttaacta gagacattag cggtctgcca
    13741 gaaacctata caaccacttt acgaacgttt aaaaagtttc tgccacagat ccaaaatgcg
    13801 cttcactaca gctattcaaa cggaccattg gaatgcttaa acaaccacat caaagtatta
    13861 aaacgcaatg cgtacggctt tcgcaacttt tacaatttca aactacgaat tatgattcgt
    13921 catggcaaaa catttttaac caaataaaca g
//
LOCUS       DACEXQ010000047        13900 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.047, whole genome shotgun sequence.
ACCESSION   DACEXQ010000047 DACEXQ010000000
VERSION     DACEXQ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.047"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            178..1128
                     /locus_tag="IXH04_001051"
     CDS             178..1128
                     /locus_tag="IXH04_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002821555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ1851674.1"
                     /translation="MLKTYSLTKDTLLVSSESNIKNSSVVYVYGDEREYIESSEHKYD
                     FKIGNILTFDDRVAYDTMIDKNDEKSLLVSFLFPEVHEGGHLDTLTTSVVFLVFKNKL
                     IIFTKQKLKFIPELLSNMVLRDVNHFVEEVLLKIMQKDFYMMLNDLRGVKEKIDDLDS
                     EISTSGSLKPTFGDLLTLQKYMIALSTTYKANHKALDFIKKNFTTIDDIDFSTKKIID
                     DLYDTSDTMDRIILSYSQYLDKLENMINNMISYQLNMIMKTLTEISIVLTIPAIIFGF
                     WGINVHVPFEKSSYGVLAVLIISAILSLICWLLMRRKTYL"
     gene            1646..2101
                     /locus_tag="IXH04_001052"
     CDS             1646..2101
                     /locus_tag="IXH04_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ1851675.1"
                     /translation="MVQQIVLPIKDSSVLKMVQDTLLDSFRAGRRNYTVFQVGKATLL
                     RVSDVMTLKKSDVYNPDGSVKNATFIHDKKTGKANTLYLKPVQQDLLQYHDWLAQENI
                     NSEWLFPSTAHYDLHITEKQFHKVMAHVGDLLGINLEDKEKKLRVRKPF"
     gene            2412..2768
                     /locus_tag="IXH04_001053"
                     /pseudo
     CDS             2412..2768
                     /locus_tag="IXH04_001053"
                     /inference="COORDINATES: protein motif:HMM:NF013878.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2781..3191
                     /locus_tag="IXH04_001054"
                     /pseudo
     CDS             2781..3191
                     /locus_tag="IXH04_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812298.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
     gene            3306..3470
                     /locus_tag="IXH04_001055"
     CDS             3306..3470
                     /locus_tag="IXH04_001055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851676.1"
                     /translation="MSYPRTDTPFITENEFAYLKANFGKYSGFLGLDLEMLLSLIMIR
                     SNNSTQILTN"
     gene            complement(3621..4600)
                     /locus_tag="IXH04_001056"
                     /pseudo
     CDS             complement(3621..4600)
                     /locus_tag="IXH04_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332307.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(4605..5372)
                     /locus_tag="IXH04_001057"
     CDS             complement(4605..5372)
                     /locus_tag="IXH04_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ1851677.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
     gene            5862..6287
                     /gene="lacA"
                     /locus_tag="IXH04_001058"
     CDS             5862..6287
                     /gene="lacA"
                     /locus_tag="IXH04_001058"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HAQ1851678.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            6304..6819
                     /gene="lacB"
                     /locus_tag="IXH04_001059"
     CDS             6304..6819
                     /gene="lacB"
                     /locus_tag="IXH04_001059"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HAQ1851679.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            6830..7762
                     /gene="lacC"
                     /locus_tag="IXH04_001060"
     CDS             6830..7762
                     /gene="lacC"
                     /locus_tag="IXH04_001060"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HAQ1851680.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTISHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            7765..8745
                     /gene="lacD"
                     /locus_tag="IXH04_001061"
     CDS             7765..8745
                     /gene="lacD"
                     /locus_tag="IXH04_001061"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAQ1851681.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            8773..9090
                     /locus_tag="IXH04_001062"
     CDS             8773..9090
                     /locus_tag="IXH04_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ1851682.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            9090..10796
                     /locus_tag="IXH04_001063"
     CDS             9090..10796
                     /locus_tag="IXH04_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1851683.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTLLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            10938..12344
                     /gene="lacG"
                     /locus_tag="IXH04_001064"
     CDS             10938..12344
                     /gene="lacG"
                     /locus_tag="IXH04_001064"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAQ1851684.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRVKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            12367..12639
                     /locus_tag="IXH04_001065"
     CDS             12367..12639
                     /locus_tag="IXH04_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HAQ1851685.1"
                     /translation="MLSDTLEKVQHLFRPGFEKVYIVSFEDCPMIPELEATPLLKCGK
                     WYVSTGKEWICHSDLELSAFEWEFLQSLDVEIRETIHFEVNYLPFQ"
     gene            12660..13559
                     /locus_tag="IXH04_001066"
     CDS             12660..13559
                     /locus_tag="IXH04_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAQ1851686.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDKDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSPLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
BASE COUNT         4513 a         2323 c         2695 g         4369 t
ORIGIN      
        1 attacaaatt agataataca ttagaagctg gagacaagat tgtctagata atgtgtgaga
       61 taaaattttt ggcaggttat catacataag acaaaatgtt tttaaaactg ataacgtatt
      121 tattccacaa caattacgca tttatttctg agaaaaatat aaagcaggag gattacaatg
      181 ctgaaaactt attccttaac aaaagataca ttattagttt cgtcagaatc aaatatcaaa
      241 aattcttctg ttgtttatgt ttatggtgac gaacgagaat atatcgaatc ttctgaacat
      301 aaatatgact ttaaaatagg caatatacta acgtttgatg atcgagttgc atatgacacg
      361 atgatagata agaacgatga aaaaagtttg ctcgtaagtt ttttgtttcc cgaggtacat
      421 gaaggtgggc atctagatac cctgacgaca tctgttgttt tcttggtatt taaaaataag
      481 ttgataattt ttacaaaaca aaaattaaaa ttcattcccg aactcttatc caatatggtt
      541 ttacgtgatg tcaatcattt cgtggaggaa gtacttttaa agatcatgca aaaagatttc
      601 tatatgatgc ttaacgacct tcgtggagtc aaggaaaaaa ttgatgattt agactcagaa
      661 atatcaacgt ccggttcact caaaccaacg tttggtgatt tattgactct tcaaaaatac
      721 atgatagctt tgtcaacaac ttataaagct aaccataaag cgcttgattt cattaaaaaa
      781 aatttcacaa ctattgacga tattgatttt agtactaaaa aaataattga tgatttatac
      841 gatacaagtg atacaatgga tagaatcatt ttaagttaca gccaatattt ggacaaattg
      901 gaaaatatga taaacaatat gatttcgtat cagctaaaca tgattatgaa aaccctaacc
      961 gagatatcaa ttgttttgac tattccagca attatttttg gcttttgggg aatcaatgtc
     1021 catgtcccgt ttgaaaagtc ttcttatggt gttttagccg tattgattat ctctgctata
     1081 ctaagtctaa tttgctggct tttgatgcga cgcaaaacat atttgtaaag tatttgtgat
     1141 accctaatat gatttaaagc agaatttctt tgttgacttt ttaaaaggct gattcctttc
     1201 aataatttaa tagcttcagc ggctgctcag aagtcaataa attggactat ttttattcag
     1261 ttacttaggc aacatttgag atacgattgc gattataaaa attgatatac caaccaatgg
     1321 ctgcttggaa gactgacata ttagcctcga tatcatcgaa taactaaata cagaaaaata
     1381 caataaatat tatcaaacta gggactatac tagaaaacat cagtacattc cttaactcag
     1441 ggtctataat tagaaaatcg cccctttgaa aactgattaa aacagccccc attatctagt
     1501 gactagataa tgggggctgt ttttttgtta agtgatataa taaaccataa aagaagttta
     1561 gaaatacggt cctggtcatc aataaataaa caatttaatt aattatttat ttaattattt
     1621 aatttcagaa aggtatacat gaatcatggt gcaacaaatt gtcctaccca tcaaagactc
     1681 cagcgtctta aagatggtac aagatacact tctcgatagt ttccgtgctg gtcgccgaaa
     1741 ttacaccgtt tttcaagttg gtaaggccac gctactacgt gtgagtgatg tcatgacatt
     1801 aaaaaaatca gatgtctata atccagacgg ctctgtcaaa aatgcaacct ttattcatga
     1861 taaaaagacc ggtaaagcaa acacgttata tttaaagcct gttcagcaag acttgttgca
     1921 atatcatgat tggctggccc aagaaaatat caattctgag tggttatttc cctcgacagc
     1981 ccattatgat cttcatatca ctgagaagca gtttcataag gtgatggcgc atgttggtga
     2041 tctcttagga atcaacttgg aagacaaaga gaaaaaattg cgagtcagaa aaccgttttg
     2101 agatatgaac agcatttgcc ttcacgcctt agcgatttta aataatagcc ttgaaagtga
     2161 ttatggaaaa gccctctacg ctcaatgcaa aattgacgta gaaactattt ttgatcgatt
     2221 gaaaggtgta tttggtatgt gaagaaccca tgtcagaggc aagaacgttg cacctactga
     2281 cattggaatg atgcttatga gcatgaatct aaccaaatta tcccttgagg ctagaagaaa
     2341 agcggaagct tttcaacaca aagcagccaa aaataaaaaa cgcgacgaag caatcacatt
     2401 tatgattatt catcgcgttt tttatattta gagctagtta tttgctagac ccactattta
     2461 cagatgaaac gtttatcacc tttggttttg ggcatttagt ggaattagca gaaccaggtc
     2521 attatgacga aaagtggcaa aattggaaac ttgaatcttt gccgattttt cctgatcgat
     2581 acgattttga agttgcaaaa gataagggaa agcagtttaa aattgttgca gaacttctca
     2641 aaaaggcaaa tacaattatt gttgcaacag atagcgacag agaaggtgaa aatatcgcct
     2701 ggtcgattat ccataatgca aatgcctttt caaaagataa aacatttaaa agactatgga
     2761 tcaatagctt agaaaaagat gtgatccgaa gcggttttca aaatttgcaa ccaggaatga
     2821 attactatcc cttttatcaa gaagcgcaaa cacgccaaat tgccgattgg ttgatcggca
     2881 tgaacgcaag ccctttgtat acgttaaatt tacaacagaa gggcgtacaa ggtacatttt
     2941 cactaggacg tgttcaaacg cccaccttat accttatttt tcagcgccag gaagccatag
     3001 agaattttaa aaaagaacct tttttcgagg tggaagctaa gtaaaacaag ggtcatttaa
     3061 gggcgttcta agccccacac agcgttttaa aacccaagag gagcttttta gcttttgttt
     3121 cttctaaaca agctaaaata ggcaatcaag aggggataat tgctgatgtt caaaccaaag
     3181 agaagaaaac gaatagtccg agttcgtttt ctttaagtag tttgcaatca aaagtcaatc
     3241 agctttataa agcgacagcg agccaaactt taaaagctat gcaaggactg tatgaagcaa
     3301 aattattgag ttatccaaga acagatacac catttattac agagaacgaa tttgcttatt
     3361 taaaagcgaa ttttggcaaa tatagcggtt ttttaggact tgatcttgaa atgttgttat
     3421 cattgataat gatacgttca aacaacagca cccaaatttt gacgaattag tgaaactcta
     3481 tgaaaaagac gtagtaaaac atgcaacatc ctattctaat cagctggtaa agcttaatta
     3541 ggttatataa gcttgaaaat aaagggtttg tccaatatgg acaagccttt ttcttatgtt
     3601 ttgccacaga tttgccacac ttattgtgta agagtatcaa ggataggtat aatttgatac
     3661 tttgtttttt tagtgatgtg caaataaatt tctgttacac gactacctct agaatgccct
     3721 acacggtctt gaattgcttc taggggtatt ccttactctg ctaagaaact aatatgcgta
     3781 tgacggaata tatgggtcgt aatatgttta ttaaaactaa gtttattatc aatatcaatt
     3841 cctgctcgtt ttggattacc ttttcgattt aattttccat tttcaggtga gttgaggaaa
     3901 ttgttaagga cactataatg aagaggtgtt ccattatagt cgggtttatt acgacttttg
     3961 gaagtaaaaa tccatccatt atctttataa tcaggattga atctcatatg ttggtgattt
     4021 aactcaattt cctctttaag aatttctata gcccttttag ataattggac atttcggtaa
     4081 ctttgtaagt tttttacatt accttcatag ctattttctg cagctcctcc gtgagtggac
     4141 tgactaatga tgatattttg cttttcaaaa tattgatagg caccaatatt ttggagtttc
     4201 gactctgtaa gtcaacaggg ccattagtta gaatacctat tgggatgcgg tttttatgta
     4261 gagccagcag tagttgatgt atctcaggaa tcatcgtaat ctgatcgagt tcatattcat
     4321 agtcagcttg gaagtctaga gcttctgtcc tagaaatact atcgatgcca aattcttcca
     4381 tagtcttctg acagcgaaaa attctaagct gctcaatact gatgagtttt ttcgtgtatt
     4441 taggaaaagc aacatcagac caatatctga aacgtttgta aatgttatct atttgttgga
     4501 tatcaatcgt tggaaaacat ttctcaacgc tacgtttaaa ggggagttga tggtcataaa
     4561 gagtgtcatc aaggtcaaaa acaatatttt tataagtcat aattctagtc cgcttttaaa
     4621 atagtagtgt actggctaaa ttcctctaag tgttgagggc taatctggtt atctgtaatg
     4681 atggtatcga gttgatctag attatagaag ttaaagaaat cgtatcgatc gaatttagta
     4741 ctgtctacta ataagaattt ttctacagca ttgtttaggg caagttgttg aatcacacct
     4801 tccttgtcac tatatgtagc aatagaatta tgggtaacag catttgcact aacaaaagct
     4861 ttggcaaatc taattgcttt taaatttgtc gaagccattg aacctacaaa agctccagtt
     4921 atttctctat attcaccgcc aagaagcaat aaatcaatgg tttcgctatc atttagaatc
     4981 aagaacacag ggagactatt tgtaatgaca cgaattttat aacctttacg acctttcaat
     5041 tctaatgcca gttgttcaag tgtagttcca ggtccaataa aaatagtatc gccatctttg
     5101 attaaagagc aggctttttt agcaatgtca attttttctt ttgtatttag acttttcttc
     5161 tcgatatgtg tcttttcaag tggcttttta gaggaaagca attgtgcacc accatgagta
     5221 cgtgttaaaa gtccatcagc ttctagagca tcaaggtctc tacgggctgt catatcggaa
     5281 atatctagtt catctattat ttcttttatg gttatggtcc catcaatctt taacatatct
     5341 aaaatttttt ctaatcgtcg ttttttgttc atatgaagac ccttctttca taaagtaatt
     5401 tttttccaaa gataattctc ttttaattgt atcataaaag acaatatttt caaggtaaaa
     5461 caaacaattt caaacaaaaa caaatgttag atgatgaaat aagaacagag gattgacgta
     5521 tattagctta ggtcagattt tgtataagac gaaaataaag taggacctct taatcagtaa
     5581 gttatagaaa gtaaaagact tttgtaatac ttgaatagat atttcacgtc cattttgtga
     5641 tggattaaat gaacaaaaat gaacaataat ttaacggtgt tatctatttt ttaaaaaaac
     5701 aaataaaaaa aacaaaaaat aaacaaaaaa agtgttgctt tttgtttgaa tgcttgatat
     5761 tatataaaca aagaaatgat gaaaacgttt tttcgaacat tttgcaaaat gttttctact
     5821 tctacgtagc atttctttct aaaatttagg aggtggtcaa aatggctatt gttgttggtg
     5881 cagatctcaa aggtacgaga ttaaaagatg ttgtgaaaaa ttttcttgta gaagaaggtt
     5941 ttgaggtaat tgatgtgact aaggacggac aagattttgt tgatgttacc ttggcagttg
     6001 cctctgaagt aaataaagat gagcaaaact taggtattgt aattgatgct tatggagctg
     6061 gtccattcat ggtagcaact aaaattaaag gtatggttgc agcagaagtt tcagatgaac
     6121 gctcagctta tatgacacgt ggacataaca attcacgaat gattacggtt ggcgctgaaa
     6181 tcgtcggtga tgagcttgct aaaaacatcg ctaaaggttt cgtcaatggt aaatatgatg
     6241 gcggacgtca tcaaatccga gtagatatgc ttaacaaaat gtgttaagaa aaggagttaa
     6301 ggaatgagaa tcgcaattgg atgtgaccac attgtcacag atgaaaaaat ggcagtatca
     6361 gaattcttga aatcaaaagg acatgaggtc cttgatttcg ggacatatga ccacgcacgt
     6421 actcactacc ctatctatgg taaaaaagtc ggtgaagcag tagtaagtgg tcaagcagac
     6481 ttgggagtat gtatctgtgg tacaggtgtc ggcattaaca atgcagtcaa taaagttcca
     6541 ggtgttcgct cagcgttggt tcgtgatatg acatcagcta tatatgcaaa agaagagtta
     6601 aatgcgaatg ttatcggttt cggtggaaag attactggtg gtcttcttat gaatgacatc
     6661 attgaagctt tcattgaagc tgagtacaaa ccaacagaag aaaataaaaa attgattgca
     6721 aaaatcgaac acgttgaaac acataatgca catcaagcag atgaagattt ctttacagaa
     6781 ttccttgaaa gatgggaccg tggtgactac cacgattaag gtataacaaa tgattctgac
     6841 agtcacacta aatccttcag tagacatctc ttatcctcta gaaacattga aaattgatac
     6901 agtgaatagg gtgaaagatg ttagtaagac agctggtgga aaaggcttaa atgttactag
     6961 agtcttgtat gaatctggtg ataaagttac tgctacaggt ttcttaggtg gtaaaattgg
     7021 tgaatttatt gaaagcgaat tagaacaatc tcctgttagt ccagcttttt ataaaatctc
     7081 aggaaacact agaaactgta ttgctatctt acacgagggt aaccaaacag aaattttgga
     7141 acaaggacca acaatttctc atgaagaagc agaaggcttt cttgaccact atagcaatct
     7201 tattaaacaa tcagaagttg tgacaatttc aggaagttta ccttcaggac ttcccaacga
     7261 ttattatgaa aaacttatcc aacttgcttc ggatgaagga gtagcagtcg ttttggattg
     7321 ttcaggtgca cctcttgaaa cagttctaaa atcgtcggct aaacctactg ccatcaaacc
     7381 taataatgag gaactttcac agttgcttgg aaaagaagta acaaaagata ttgaagaact
     7441 caaagacgtt cttaaagagt cactttttag tggtatcgaa tggatagtgg tctcattagg
     7501 tcgaaacggt gcctttgcaa aacatggtga tgttttctat aaggtagata ttcctgatat
     7561 cccagttgtt aatccagtcg gatcagggga ctcaacggta gctggtattg catcagcttt
     7621 aaatagtaaa aaaagtgacg ctgacttatt aaaacatgcg atgacattgg gtatgttaaa
     7681 tgctcaagaa acaatgacag ggcatgtgaa tatgactaat tacgaaacac taaactcaca
     7741 aattggagta aaagaggtat aaaaatggta cttacagaac agaaacgcaa atctttggaa
     7801 aaaatttcag ataaaaacgg tgttatctca gctttggcat ttgaccaacg tggtgctttg
     7861 aaacgtttga tggcacagta tcaagataca gaaccaactg tagctcaaat ggaagaacta
     7921 aaggttttgg ttgctgatga acttacaaaa tacgcttctt caatgttgct tgaccctgaa
     7981 tatggacttc cagcaacaaa agcattggat aaagaagcag gtcttcttct tgcctatgaa
     8041 aaaacaggtt atgatacatc aagtacaaaa cgtttgcctg actgcttgga tgtttggtct
     8101 gcaaaacgta tcaaagagca aggcgctgat gccgttaagt tcttgcttta ctatgatgta
     8161 gacagctcag atgaacttaa ccaacaaaaa caagcttata ttgaacgtgt tggttctgaa
     8221 tgtgtcgctg aagatattcc tttcttcttg gaaatccttg cttatgatga agaaatctca
     8281 gatgcaggtt ctgttgaata tgcaaaagtg aaacctcgca aagtcattga agcgatgaaa
     8341 gttttctctg atccacgctt taacattgat gttcttaaag tagaagtacc agttaacgtt
     8401 aaatatgtcg aaggttttgc tgatggtgaa gtagtttaca gtaaagctga agctgctgac
     8461 ttctttaaag cacaagaaga agcgactaac cttccataca tctacctaag tgcaggtgta
     8521 tctgctaaat tgttccaaga aacattgcaa tttgctcacg actcaggtgc caagtttaat
     8581 ggtgtgcttt gtggacgtgc tacttgggca ggatctgtag aaccttacat caaagaaggt
     8641 gaaaaagcag cacgtgaatg gttgcgtact actggattcg aaaatattga tgaacttaac
     8701 aaagttcttg ttaaaacagc tagtccatgg actgataaag tatagtaaaa acataaaacg
     8761 gaggatattg ttatgaacag agaagagatg actctcttag ggtttgaaat tgttgcttat
     8821 gctggagatg ctcgctctaa acttttagaa gcgcttaaag cggctgaaaa tggtgatttc
     8881 gctaaggcag atagtcttgt agtagaagca ggaagctgta ttgcagaggc tcacaattct
     8941 cagacagcta tgttggctcg agaagcttct ggggaggaac ttccatacag cgttaccatg
     9001 atgcatggtc aggaccactt aatgactact gtcttattaa aagatgtgat tcatcacctc
     9061 atcgaacttt ataaaagagg agcaaaataa tgcataaact cattgaactt attgagaaag
     9121 ggaaaccgtt ctttgagaaa atctctcgaa acatctatct tcgtgctatt cgtgatggat
     9181 ttatcgctgg tatgccagtc atccttttct catctatctt tatccttatt gcctatgtcc
     9241 caaatgcttg gggattccac tggtcaaaag acattgagac cttcttgatg actccttata
     9301 gctattcgat gggtatccta gcattctttg ttggtggtac taccgctaaa gctttgacgg
     9361 actcaatgaa ccgtgaccta cctgcgacca atcagattaa cttcttatct acgatgctag
     9421 cgtctatggt agggttcctt ttgatggcag ctgagcctgc aaaagaagga ggcttcttga
     9481 cagccttcac gggaacaaaa ggtcttttga cagcatttat tgcagccttt gtgacagtta
     9541 atgtttacaa ggtctgtgtg aaaaataatg ttactattcg tatgcctgaa gaggttccac
     9601 caaatatctc tcaagtattt aaagacttga ttccattcac tgtagcggtt gttctccttt
     9661 atggatttga actcattgtt aagggaactc ttggtgtaac cgttgcagaa tcaatcggta
     9721 cccttcttgc tcctctcttc tcagctgcag atggttacct tggaattaca cttatctttg
     9781 gtgcttatgc cttcttctgg tttgttggta tccacggtcc ttcaattgtc gaaccagcaa
     9841 tcgctgctat cacttatgcc aatatcgatg ccaacttgca tcttatccaa gctggacaac
     9901 atgcagacaa agttatcact tctggtactc aaatgtttat tgttaccatg ggtggaacag
     9961 gagctacatt gattgttcca ttcttgttca tgtggatttg taaatcagaa cgtaaccgtg
    10021 ccatcggacg tgcctcagtt gttccaacat tctttggggt taatgagcca atcttatttg
    10081 gtgcaccaat cgtattgaat ccgattttct ttgtaccgtt cattttcgct ccgattgtca
    10141 acgtttggat ctttaaattc tttgttgaca cattgaacat gaactcattc tctgccaacc
    10201 ttccatgggt tactcctggt ccgttgggaa ttgtactcgg tactaacttc caagttctat
    10261 catttatctt agccggactc ttggtagttg ttgatactat catttattac ccatttgtta
    10321 aggtatacga tgaacaaatt cttgaagaag aacgttctgg taaaacaaac gatgctctta
    10381 aagaaaaagt agctgcaaac ttcaatactg ctaaagcaga tgccgttctt ggaaaagcag
    10441 gcgttgctaa agaagatgta gcagcaaaca ataatatcac aaaagaaact aatgtccttg
    10501 ttctttgtgc aggtggaggt acaagtggct tgcttgccaa tgccttgaac aaagcagcag
    10561 cagaatacaa tgttccagtc aaagcggcag cgggtagtta cggtgctcac cgtgaaatgt
    10621 tgccagagtt tgacttggtt atcttggctc cacaggtcgc ttcaaacttc gatgatatga
    10681 aggctgaaac ggataaattg ggcattaaac ttgcaaaaac tgaaggagct caatatatca
    10741 aattgacacg tgatggacaa ggtgctcttg catttgttca acaacagttt gattaataaa
    10801 tttgtaaaag ttatttgaga cgagattatt ctttcaattc cttataatat tgttaaccat
    10861 atcattgcat ttcatttcct tggtttccgg aactcagaat atcgtctcaa atgctttttt
    10921 tgaaaggatt tatagttatg actaaaacac ttcctaaaga ttttattttt ggtggtgcta
    10981 cagctgctta tcaagcagaa ggtgcgactc acacggatgg taaaggccca gttgcatggg
    11041 ataagtatct tgaagacaat tattggtaca cagcagagcc tgcaagtgat ttttaccata
    11101 aatatcctgt tgatttagaa ttagctgaaa aatatggcgt taatggtatt cgtatttcaa
    11161 ttgcctggtc tcgtattttc ccaactggct atggcgaagt aaatgaaaaa ggtgttgaat
    11221 tctaccataa tctttttgcc gagtgccaca aacgtcatgt tgagccattc gtgactttgc
    11281 accactttga cacaccagaa gttcttcact cagatggaga tttcttaaac cgcgaaaata
    11341 tagaacactt tgtagattat gccgctttct gttttgaaga gttcccagaa gtaaactact
    11401 ggacaacttt caatgaaatt ggcccaattg gagatggtca atacttggtt ggtaaattcc
    11461 ctccaggcat tcaatatgat cttgccaaag tcttccaatc acaccacaat atgatggttt
    11521 ctcatgcacg tgctgtgaaa ttgtataaag ataaaggtta taaaggcgaa attggtgtag
    11581 tacatgcgtt gccaaccaaa tacccttatg atccagaaaa tccagcagat gttcgtgccg
    11641 ctgagttaga agatatcatc cataacaaat ttatcttgga tgcaacctac cttggacact
    11701 attcagataa aacgatggaa ggggtcaacc acatcctagc tgaaaatggt ggagaacttg
    11761 atcttcgtga tgaagacttc caagcacttg aagcagctaa agatttgaac gatttccttg
    11821 gtatcaacta ctacatgagt gattggatgc aagcctttga tggtgagact gaaatcattc
    11881 acaatggtaa gggtgaaaaa ggaagttcta agtaccagat taaaggtgtt ggtcgccgaa
    11941 tcgcaccaga ttatgtgcct agaacggatt gggactggat tatttatcct gaaggcttgt
    12001 atgaccaaat catgcgagtg aaaaatgatt atccaaacta caagaagatt tacatcactg
    12061 aaaacggtct tggatacaaa gatgagtttg tagataatac cgtttatgat gatggtcgta
    12121 ttgattacgt gaagaaacac ttggaagttt tatcagacgc aattgcggat ggtgcaaatg
    12181 ttaaaggtta cttcatctgg tcacttatgg atgtcttctc atggtcaaac ggatacgaaa
    12241 aacgttatgg actattctat gtagactttg acacgcaaga acgctatcct aagaaatcag
    12301 ctcattggta taagaaagta gcagaaactc aagtgataga gtaatattac ttgaaaatgt
    12361 aaaaaattgt tatccgatac tttagaaaaa gtacaacact tatttagacc aggctttgaa
    12421 aaggtatata ttgttagttt cgaagattgt cctatgattc ctgagcttga agctaccccg
    12481 ttgctaaaat gtggtaaatg gtatgtttca actggaaaag agtggatttg ccattctgat
    12541 ttagagttat cagcatttga atgggaattt ttacaatcac tcgatgtaga aatacgtgag
    12601 actattcact tcgaggtaaa ttatttgcct ttccaataat aaaaagaaag gaattagttg
    12661 tgactatcga attaaaaaac gagtatctta ctgttcagtt taaaacactt ggtggtcaac
    12721 tgacttcgat taaagacaag gatgggatag agtatctctg gcaagctgat ccaaattatt
    12781 ggaatggaca ggcgcctatt ctatttccta tctgtggtag tttacgaaat gattgggcta
    12841 tttatagacc tcaagaaaga ccctttttta caggtcttat tcgtagacat ggttttgttc
    12901 gaaaagaaga atttattctg gaagaagtca atgataatag cgtgaccttt agtattaaac
    12961 caaatgctga gatgcttgat aattacctat atcagtttga actgagagtt gtttatacct
    13021 tgaatggtaa atcaattaga accgaatttc aggtaacgaa cttggaaatg gaaaaaacaa
    13081 tgccttactt tattggagcc catccagcat tcaatagtcc tttagtagaa ggtgaaaact
    13141 atgaggatta ttatattgaa tttagtgagg ttgagtcttg ctcgatacca aagagttttc
    13201 ctgagacagg attattagat ttgcaagatc ggacaccttt tttggaaaat caaaaaattc
    13261 tcgatttgga ctactcactt ttttcccatg atgccattac acttgaccag ctcaagtctc
    13321 gtagcgtaac gcttcggtca aggaagtctg gtaaaggtct tcgagtagat tttgatgatt
    13381 ttccgaactt gattctgtgg tctacaacta ataaaagtcc attcattgct ttggaacctt
    13441 ggagtggtct ctccacttca cttgaagaag gtaatttctt agaagataaa agacaggtaa
    13501 cgaaaatttc acctcaagaa acttctagaa aaatctatga tattactatc cttaattaaa
    13561 atttgttatt atttgagtct tcaataagat ttgaatactc ttaccttgtg atgatagatt
    13621 ggtcttctcc gtgccagtct tacatcgcaa ggtttttaag ttttttgtgg gtctatgtca
    13681 actgtagtgg gtgacgaaaa gctaatatct aaagacagtc tggtagtaaa aaataagatt
    13741 atttactact tcttgattta aaataatagt ggagccatta atttgtggca aatttaattt
    13801 aatattttgc ctcaaattag ccacactttt tatagaacta agaggaaaaa tgaaattttc
    13861 ttcctattaa tatagttgtt tgaaatctta aatatagcgt
//
LOCUS       DACEXQ010000048        13817 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.048, whole genome shotgun sequence.
ACCESSION   DACEXQ010000048 DACEXQ010000000
VERSION     DACEXQ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.048"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            476..1339
                     /locus_tag="IXH04_001067"
     CDS             476..1339
                     /locus_tag="IXH04_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1851687.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="IXH04_001068"
     CDS             complement(1468..3090)
                     /locus_tag="IXH04_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1851688.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="IXH04_001069"
     CDS             3339..3860
                     /locus_tag="IXH04_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851689.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="IXH04_001070"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="IXH04_001070"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAQ1851690.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="IXH04_001071"
     CDS             5941..6759
                     /locus_tag="IXH04_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAQ1851691.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="IXH04_001072"
     CDS             complement(6862..7059)
                     /locus_tag="IXH04_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851692.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="IXH04_001073"
     CDS             7228..7806
                     /locus_tag="IXH04_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAQ1851693.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            complement(8851..9354)
                     /locus_tag="IXH04_001074"
     CDS             complement(8851..9354)
                     /locus_tag="IXH04_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ1851694.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9713..10738
                     /locus_tag="IXH04_001075"
     CDS             9713..10738
                     /locus_tag="IXH04_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ1851695.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            11085..11963
                     /locus_tag="IXH04_001076"
     CDS             11085..11963
                     /locus_tag="IXH04_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851696.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            12439..12555
                     /locus_tag="IXH04_001077"
     CDS             12439..12555
                     /locus_tag="IXH04_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAQ1851697.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13483..>13817
                     /locus_tag="IXH04_001078"
     CDS             13483..>13817
                     /locus_tag="IXH04_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851698.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILW"
BASE COUNT         4361 a         2333 c         2635 g         4488 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatagtta gtttttccac agtggataac ttcgtaagag ataaaaataa aaaattagtc
     8221 aaagaagggc gtatagattt cttttgatag gagatataca acagaaatga ataaatatta
     8281 acgtgttttc aaacagtata aaacggtgtg gtttttggaa taaaaaaata acatatctca
     8341 ttttacgctt ttgttatttt atacttaaaa aataacgaca cagaaaatac cgtgtctcag
     8401 atttctctac tacttctaaa aactactgtt tttttccttt gaaatgcctt caaaaagtag
     8461 gatcaggcag aatatactgg cagaatcaag gagaagggag cctaaataag gagaaaaacc
     8521 atagaaaata taaagtaatt acttataaaa caagcttttt atagaaaaat aatatacctg
     8581 agtagggtat aaattaagac ggaattcctt tctagattga gatattgact aggaagaatg
     8641 ttaagatttt aagcctttaa gctaaaaaaa aacccttttt gttaaataag gggaatatag
     8701 cctgtcagtt tcaaactgca agtcgtcctg ctgcaataat atagtagatt agagatgaaa
     8761 atagattttt ttaaaccaat gactgaataa aaagaccgtc atagatacac attggaatgt
     8821 gtctattacg gtcttttttt acgaacgatt ttattgaaga cggacagttc gggtcaaaaa
     8881 taaaaaagct acgatcagaa ataagaataa aatgtatccc aaaataatgg ctagacggtc
     8941 taacttattt gtcgatagat gtttgtttag aagttgatag gcccataaac ctatcagtcc
     9001 tcctgttgta ttagttatca aatcagtgat gtccgctacg ccaataccga ataggaattg
     9061 aatcaattct gccaagaagc ttacaaggaa aataatccct aattttttag aaaaagaaag
     9121 tctcttgaaa ttgacattca aaagcacccc aaaaggaaaa aagacaatca agttataagt
     9181 ggtctcacgt aataccgtcg tattttcaaa agaaaaagta gaaaaaggga cgagattgac
     9241 attgctgtaa gaataagtga gaaattgtgg aagattaaat gatagcttga ataaaatcaa
     9301 ccataataga aagaataaat aaatccctaa taaaaatttt gatacatacc gcatagacat
     9361 ctcctccatt taaaaagtat accatcgaaa accgctgaaa aatcataaaa aagtatgaag
     9421 tttaaggacc aacgtagtta tcgtacgaaa attagaagct tttttttgtt gggaatccgg
     9481 tttctttaac aaagctaaga tagcgagttg cttggaaaag ttgctatctt ttttctgtca
     9541 aaacgactcg gaatgttcgt tttttgttct aatttctcca agttgtttac tttttgatac
     9601 taagtttatg aaaaatcaag aaatagttgt tatactaggt aatgctgaca aacgggattg
     9661 gcatttcatc ttggttcagc tttttttcca attactagga aggagggata gattgttcaa
     9721 gacttatttt cgtttcaata aaatacaaga taatcctttt cgtatcatga atctgatgct
     9781 acttatcttt attgtattat ggttaggtta cataaatcat gattttttga tttcaagttt
     9841 ttcatcatta gggatcttta cttttttcta ttatcaaaac ataccgatga aatatttgct
     9901 gaaacgtatg tttctgatcg gttgcggact gttggttgct ttcgttttgg ggatcttatc
     9961 gacttatgtt ttatggcttg aaccatttgc cgtagctttg gttgcttttt ccagcagatt
    10021 cgtgttgcga ttattccata tttctaaacc aggtggcttg ttttttgcga tgctttctgc
    10081 catgggaacg agcatgcagc tgccaatcgc tcaattaccg atcgtctcac tttatttttt
    10141 tatgggtgtc gtttttgccc tgattgcggc agtgattaca aaattactgg attcacggcc
    10201 tgagcagaca attgaaaaag caactttaaa agaaagattt cacgaagagc ctttagtgat
    10261 cattgattct gttttctaca gcgctgcctt atttctttcc gtttatgtca gtcacggctt
    10321 gaatctgcat aatccttatt ggttgacctt gtcatgtgca tcgattttgc tagcagaaaa
    10381 cttggatgcg atgaagcatc gacaagtcca atatctgatt ggatcgatgg gtgggttatg
    10441 tgtgtcggca ttcttgtcat ttgtgccgtt tacgcagttg cagaccattt ttctgatcac
    10501 ttttctatat ggaatcgcac aatttctagt tgctagaaat tatgcagttg ctaatatttt
    10561 tcttaatccg atggcgttga tgctatctac acttattcgt ggagcatact tgatttcttt
    10621 gatcgaatat cgttttttag ggatcgtcat aggaagtttc atcggtttag gtgttgcgtg
    10681 ggtaatgacg gttggtttgc aacattattt aacggttgtt cgtcaaaaat tagaatagaa
    10741 ggacaaatat atgtgccaaa agaggttatg acaagatttt tgtcacaacc tctaattcaa
    10801 ggtgttcaaa agccagattc tcagcattaa gtcaaattca cacaaaaatg tggaaaggaa
    10861 ctttcaaaaa aattttatat ttattctatc aaaaaatttt atatttattc tatcaaaaaa
    10921 ttttgtttat gatggtaact gaataaactt tcctgagatg gtcaatgctt tccttgaaag
    10981 tcttacagat attctttctt catagaaagt ttaagtttta tagttagtgt acaataaaag
    11041 tatgtttata ccatatggtg tccggataat cagtcattta aacaatgagg ttcaaagata
    11101 tgaaaagggg aaatttgatg aagaaaataa gcaatgaaat ccaaattctg gcagaaactt
    11161 taactcttcc caaagtcgtt atagagcaaa tgattgtaca agcaaaaaac atccttgatg
    11221 aggtgtttga gcaatatagt catgaactaa tggatcctaa taaagcaaag gcgactgcaa
    11281 agaaaataga caaagtctat gaagaggaac cttttgcagc attagctatt tatctatatg
    11341 cagcttctca aagttggaaa aaaatttatc aaccattgaa gattccaaga actatctatt
    11401 tggcaacaat gaatgctttt actagattta ttcatgaaca tttccaagta acaggaagct
    11461 atcgctttga ccgtggtttt tggatttggc attacactag tggcttgatc tttcggattg
    11521 gtgaattgga gtacgaaatg acatattttc cgaaagggta cacggatcta agattagaag
    11581 gaaagcagtg cctttctctt catattcctt ccgatgcaga tttatctcag aataagataa
    11641 gagatagtta cgaaagtgcc attcgttttt ttgaacttta ttttcctgat taccattacc
    11701 aagtgatgta cacagatact tggttactat cacccaatct tactaaatgg ttgaaaaaag
    11761 agtcaaaaat ctgtcttttt gcagccgatt atcgattatt atcagttgat gaacaggacg
    11821 atagtggagt cccttggatc tttggacgag tagatgccca aatacatgat tatccagaaa
    11881 gtacgagttt acagcgacaa gcaaaagagc aattgcttgc aggcgagcac attggctctg
    11941 gcttaggtat tcttacttca taagtttagt acttcgaaat agtttaaaag agcgtgagac
    12001 aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg aagcagaagc
    12061 aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt cgtgaatttc
    12121 ttcttatttc ttggagttaa acgcttctgt cccagcctct tttttagtgt tctatcgaac
    12181 acacagtacg ttcaataggt aaactagcta taacaataga tattaaagtt aacggacaag
    12241 agggcctttt ttaggtagct aaaacgtaat aatgaaaatt tatttccaga gataggagtc
    12301 aatcagctag tctgatcgac tgttttaaca agttgacttt cattttaact tttgatacca
    12361 tttaaaacac aaatcgtaat tgtttcgatt tgtgtttttt agcatgacta caatcaaaga
    12421 agaaagtggg tcgtccatat ggaaaaaaca gatatctcaa gtgcataccg ccgcttgaaa
    12481 agcccaaata tcaaaacacg aaaacgtgcg ctgaagatca tcaaagatgt gaaacggaat
    12541 agtgggaaaa ggtaagaaga aggaaactta attaaaaaaa tagccgaaaa acgtttttcg
    12601 gctattttca ataatcggtt tttgtgtaag gtttccgaaa gaactgctgt tgattttcca
    12661 tttttaacat atgataaatt ggtagaatct ttcttttcaa attgatacca ttccctaatt
    12721 ccagttatat agttaactac agaaccacaa agcacgtacg tttataaaag tattatctgt
    12781 agttattttt caattccctt tcaacctctt attttgacca gtagtattga tcactttacc
    12841 ttttaacacc tttgttcgtt tttgaaacat ttttagtcaa gaatggaaac gtaactacat
    12901 aatcaaaggt tttgcgaaat atttcatgaa atatttcatg tagaagcgag caatacggtt
    12961 gcaaataacc attatcgttt aataagcagt tacgaaagct cccaatatct ctaacgttca
    13021 gctcactcca tttcttttct tcaaaaaaat cagtgattcg tatctctttc tatttgtcga
    13081 aaaagttaac tttaaaaagt aacgccgaaa aaatatgcaa aaaaataaaa aagcagttac
    13141 tattgctaga ataacatcaa ggaataataa gttttggttt tcagataata gtcgataagt
    13201 tgtgtattgg aattttagat acagtaataa agtatttttc atgaaatttt gtgagaatca
    13261 ctattacaag tatctttaat aaaattaatg gttaaggagg aacttaaagt agaatgtaaa
    13321 tgaatagttg tagtcagtcg attgtgatac tagactcaaa gttgcaggaa taagtttttc
    13381 aatttgtgat tggaagataa aaaatgaaaa agaagggctt ttttttatac tttgttgatt
    13441 gtagtgttta ttttccctct gtttagccaa gtaacatttt ctatgactca accagtttca
    13501 agtgaggaac atgataacct ttttcgtgct acagaagagt ttaagaaata tcaggaaaat
    13561 attgttccgg agactccatt agctagtgag ccaattactg atattgatgg aaaccaaata
    13621 ggatggtata tacagtacga agttatggtg gtaaataatg actcaatagt aaaaaataca
    13681 gtaggcttag agctaacacc gattgaaaag gtgagttcat tgttaacgta tatttatact
    13741 gatgaaagtg acgagactga gatcatttta acggattact caaaaattgc tactgataaa
    13801 aagtatatat tgtggat
//
LOCUS       DACEXQ010000049        13766 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.049, whole genome shotgun sequence.
ACCESSION   DACEXQ010000049 DACEXQ010000000
VERSION     DACEXQ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13766
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.049"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..453
                     /locus_tag="IXH04_001079"
     CDS             <1..453
                     /locus_tag="IXH04_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003243232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAQ1851699.1"
                     /translation="GVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKP
                     LFIAENGLGAIDKVEEDGTVQDDYRIDYLKQHIEQMKEAIEDGVDLFGYTSWGCIDIV
                     SASTSQMSKRYGYIYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            1037..1471
                     /locus_tag="IXH04_001080"
     CDS             1037..1471
                     /locus_tag="IXH04_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1851700.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            1572..2663
                     /locus_tag="IXH04_001081"
     CDS             1572..2663
                     /locus_tag="IXH04_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAQ1851701.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            2693..3370
                     /locus_tag="IXH04_001082"
     CDS             2693..3370
                     /locus_tag="IXH04_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAQ1851702.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            3702..4997
                     /locus_tag="IXH04_001083"
     CDS             3702..4997
                     /locus_tag="IXH04_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ1851703.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVKEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            4987..6447
                     /locus_tag="IXH04_001084"
     CDS             4987..6447
                     /locus_tag="IXH04_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1851704.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            6583..8079
                     /gene="glpK"
                     /locus_tag="IXH04_001085"
     CDS             6583..8079
                     /gene="glpK"
                     /locus_tag="IXH04_001085"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAQ1851705.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            8093..9916
                     /gene="glpO"
                     /locus_tag="IXH04_001086"
     CDS             8093..9916
                     /gene="glpO"
                     /locus_tag="IXH04_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAQ1851706.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            9917..10633
                     /locus_tag="IXH04_001087"
     CDS             9917..10633
                     /locus_tag="IXH04_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ1851707.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            10939..12867
                     /locus_tag="IXH04_001088"
     CDS             10939..12867
                     /locus_tag="IXH04_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAQ1851708.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            12993..13547
                     /locus_tag="IXH04_001089"
     CDS             12993..13547
                     /locus_tag="IXH04_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAQ1851709.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
BASE COUNT         4614 a         2302 c         3101 g         3749 t
ORIGIN      
        1 ggggtccaag gaaacttaat aactggagga attaaaaatc cgtatcttcc atcaagtgaa
       61 tggggctggc agattgatcc taagggtttg agaattgctt tgaatcaatt atatgatagg
      121 tatcaaaaac cactgttcat agctgaaaac ggactcggag ctatagacaa agtagaagaa
      181 gatggaaccg ttcaagacga ttaccgcatt gactatttga agcagcatat tgagcagatg
      241 aaggaagcta tcgaagatgg agtagattta tttggttata cgtcgtgggg ctgcattgat
      301 atcgtcagcg cttctacttc acaaatgtca aaacgttatg gatatatcta tgttgatcaa
      361 gatgacgaag ggaatggcac aaagaaacgg ttaaagaaaa aatcatttga ttggtacaga
      421 caagtcatcc aatcaaatgg cgaaatccta taataaaagg aaataaggga ctgtgacaaa
      481 agtcttgtca cagccccttc tttacgaata aacagtattc tcctgccaac tcctcgatca
      541 caagtcacca tagtcataaa acatgagaag ctgttttttg aaattgctcc ttgtgactcg
      601 gagttcaacg gcaggctcac gacctctcaa taaacggtgt acaatcagct gaccatcggc
      661 attagctcaa aaaacgacaa aatatgagga actatttttg tttggctgtt cttattactt
      721 gatgcaggac ggctttttca caacctctga tataaacggt gttcaaaagc cagtttttcg
      781 gtattaagcc aaatttatgc aaaaatgtgg aaagccattt tcacaaaatt tttcttcata
      841 ctcaaaacta aacgactttt tcacaacctc tgattcacgg tgttccagaa ggagttttgt
      901 cgaaaacaag ccgaaattct ttgaagatgg ctacttcttt ttttcaaatc tagacagagg
      961 aggcggacat tattccgtgt tttttgacaa aaaataaaaa tcatactatg atatgtacgc
     1021 caatggaggg gatgaaatgg aaaagccaat tgaaaaagcg ttagatgaat gtcttttctt
     1081 tagcgtgaaa aagctagatc gcatgttgaa taaattagct gatgaagcgt ttagaaggac
     1141 aggtctggca ccaacttatg gttttatttt attgatatta aaagaaaaag atggaatacc
     1201 acaaaaagat attgcacaaa tgctctattc cgccccatca acgattgctc gttttgttga
     1261 aaaattagaa tataaaggat acgtcaaaac agttagcgaa ggtcgcttgt cattagttta
     1321 tcttacagaa caaggacgcc aatttgctaa ggaagtcgat ggtagctggg aagaacttca
     1381 tcagtcatac aaagcagttt tgggaaatga agcaagtaaa gaattagccc agacaatgaa
     1441 tgatgcaaca gataagttac aaaaaaaata aaatttttta agcaaattag ttgcatgtac
     1501 aactaatcga gtggtttaat gatagatgat tctcgatagg ttaatcagag cgaattatgg
     1561 gaggaatgac aatgtcacaa tttgatcaat caattacttt accttcagga gttcaattga
     1621 agaaccgatt gatgctgtcg cctatgacga cacaactcag ctttttcaat ggagtgatca
     1681 ccgaagatga gatcacttat tataaacaac gttctaaagg tcttggtgct gttattactg
     1741 gtgctgcaaa tgtccaagat atcggaaaag gctggccagg tgaactcagc atcgctcatg
     1801 atgaaatgat tcctcgatta agtgagctag cgaaagcaat ccaaggagaa ggcgcaaaag
     1861 cgattattca gattttccac ggtggacgaa tgacaagtcg agcaacttta tctggagaac
     1921 agcctgtatc tgcaagtgca gttgcagcaa aacgtcctga tgcggaaaca ccacgagcaa
     1981 tgagtgaaga agaaatcgtt gaaacaatcc aagcatttgg caaagccaca cgtagagcga
     2041 ttgaatcagg atttgatgga atcgagttgc atggagcaaa tacgtatttg atccaacaat
     2101 ttttctctcc gcattcgaat agacgagagg atcaatgggg cggtagcttg gaaaatcgtt
     2161 atcgttttat tgaagaatta ttaacaacgg ttttctcagc ggttgaacaa tatgctaaaa
     2221 aaccattcat cgtagggtat cgtttctcac cagaagaata tgaaacacca ggtatccgtt
     2281 ttgaagacac gatctggctt cttgaacgat tacgtgagtc aaaactagat tatctgcatg
     2341 tttcattaaa tatgtatgat cgaatagctc gttctgataa atacaacgat aaatcgattc
     2401 ttgaagtggt ccataacacg attcaaggaa agatcccttt agttggtgtc ggtgggatcc
     2461 gcaatcgcga agatgtagag caggtattga aatatgcaga acttgcagca attggacagc
     2521 aaatgatcgt cgatcctgac tgggcagaga aattattgga aaaccgtgag gatgaatttg
     2581 taacgaaacc ttttgaagaa gcatataaag agctttattt gccaagccca ttatataatt
     2641 tcttgaaagc acgttatcaa taacgcaatt tttatagaaa gaggatggga aaatgaaagt
     2701 attgatcgta ttaacaaatg tagaaaagta cgaaaagatc gagcgtccta caggtttgtg
     2761 gttaagtgaa ctgactcatt tttatgatgt attagtaaaa aatgggatcg atgcagattt
     2821 tgtcagtcct aaaggcggat atgtaccttt agaaccgcaa agtgttttga atatggatga
     2881 aatcgattgg gcctattatg aagatgaaga tttcagaaat cgggccttgg gaaatacctt
     2941 acgtcctgat gatgtggatg caaatgatta tgcagcggtt tattatgctg gtggccacgg
     3001 aacaatgtgg gattttccag aagcaataga tcttgggaaa gtcgcttcac aaatctatcg
     3061 aaatgaaggg ctggtctcag ctgtttgtca cggtgttgta ggattgttgc cagttacaga
     3121 tgagtctgga aaatctattt tagctggacg tacagtgact gggttcagta atgaagaaga
     3181 ggaagcaaat ggtacaacga atgaagtacc attcttagca gaagatgctt tgaaagaaaa
     3241 aggagcaaac tatcagtctg gagcagcttt ttcagaagtg gttcaaattg atgggcggct
     3301 tcttacaggg caaaatcctc aatctgctca tcaattaggc gaagcagttg ttgaagcatt
     3361 aaagaattaa aaataaatga tgtgtctatt ctaactatta cattacacac gctaaaactc
     3421 gtcttttcgt tcttttctgt ttcctatttg gacagaggta cgtaaaaggc gagttttttt
     3481 cttttataag agtacatttt ttgaaacatc tcttcagaaa tgatactctt caattaggtc
     3541 cactagtaca ttattgagaa gcagacctag aacacggtat gatggagagg gttgattctg
     3601 ctgcatcgct atgtatcata aggttgaaag cgagttatga atactgtgtt ttctgattga
     3661 gaagttagca gtagaacacc ttataagaaa gaggaatgca gatgaacatt gtaattgtcg
     3721 gcgggtcgtt tgcagggatt catgcggcta tttatcttcg ccaattgatg ccaacaagtg
     3781 aaatatattt gattgaaaag caatcgaagt taggatggat tcccagcggg ttcaatttga
     3841 tattaaaagg aaaggttacc agtgaaaaac aactttcttg gatcacgaaa gaagaactga
     3901 ctgaccgcta tcgaatccat gtttacacag aaaagacagt aataggattg aaagaaaaaa
     3961 aagtcgtttt agcaggagga aaaaaattgg attttgatcg tttgattcta gcgactggtt
     4021 caaatcaaaa gtttcgaaat atttctgcag aatcaacgct tattcaccca gttaaaaaga
     4081 tacaaaacgt aacggctctt cagcaaaaga tcatgaatgc aaagaaaatt gcaattattg
     4141 gtgcaggaca agcaggaatc gaaatagctg aagggttggc atcaagaaaa aaacaaatcc
     4201 atctttacga aagccgaaag tcaattcttt ttcgttatct agatcctgaa atgacagaac
     4261 cgttagtaaa ggaaatgaaa aatcaagggc tttcactttt tttagaagaa caagttgtgt
     4321 ctttgaccga aaatgaaacc gctattgtag agacggaaaa aagaaaggag aactatgatc
     4381 ttgttttgct ggctaatcat tctcgccccg acaatcagat gtgggaagaa caattaacat
     4441 tgaatgatga tggaacgatc tgggtagatg actacttgca aacgtctcaa aaagacgttt
     4501 atgcgattgg cgatgcgatc caagtaactt ttcgtccaac aaatgaaaaa atgtatgttt
     4561 ctctagtaaa taacgctgtc cgtacagcac gaaatgtcag caagacaatt agtggctcac
     4621 agacaaagga tcagggaacc tatcgtccag tcggcaatca gtggtttggc tatttcttag
     4681 gaagcgtagg tttgacagaa gaagagagca ttttttatcc ccaaaaaatt gaaacaggat
     4741 acgtagacta caagcttaca gcaataaatc aaagcaaagt taaactcaaa tttctatttg
     4801 attctgaaaa aaagattctt ggagcacagc tccaaagcaa aaaagagatg ttccaccttc
     4861 ttgaccaact gacaattgca gtaaaagaag gatggacgtt tgaacagtta gaagaacatg
     4921 agctattttt ccagccagaa taccgtgtgc cagatatggt gtggtcaaag gtggcgaata
     4981 ctgcctatga agattgaagc attattagaa agaaaagagc agttacagat cctgattttg
     5041 cgaaaccttg ttctgcaagg gggaacagcc tctatcaatg gtttgagaga gcatgtccaa
     5101 ctgtcaaaag cctcgtttga tcaatatttg gaagatattg aattgattgg ccgaatgatg
     5161 gaaaaaaaag tagaagtaca gcgaaatgag tatcaggcgc tgcttgtgtt ggacgaagat
     5221 gtaagtttgg aaaaaattct attgttttta ttgcaggaaa gcttgaagtt taagatgctc
     5281 gtctatttat tagaacatca gcaagtatcc atcgttcgat tagcgacagc gtttaatatc
     5341 agtgagtctt ctgtttttcg aaagatcaaa gaattgaacc agctcttaga ggaatttgga
     5401 ttacaaatca aaaacggtca attatacgga gaagagctac agatacgata tttttattat
     5461 gaactattcc aatatatccc ggaagatcag cgaccgcttt ttttgcaaaa tacaccagaa
     5521 aaaagaccgt tcattctagg attggaccgt gtattagaaa caacgtttac ggcatcagca
     5581 gaagcacaga ttgcttgctg gctcgggatt acgaaaaaaa gactattgaa tgaaaaaagt
     5641 acgtacgcaa ctttgaaaga aaagaaatta ctctatcagt cggaccggct ttatcaagca
     5701 atcgatccaa tcatcgtgat gcatcttagt cgaacagcag cagaactgaa tgtatacgaa
     5761 acaatgatgt tttacagctt ttttgtttct ttttcgatag tagacgaaga aatattttat
     5821 cagtatgatt tgacacggag caaaaagtta ccaaccgctg ttttagatac ttacattcgg
     5881 gaaacgatgt tatggcatta tcgtcctcgt cgtttgaaaa tcaaagaaga aaaggcagta
     5941 ggctaccaaa tttcccagat caacaatgaa tggtttttct ttgttggaaa gatcgaagtc
     6001 tatgagcgtg atcggctttt ggaacagcag cagaaaatgc tcgggcattc cttaacacag
     6061 ttactggcga aattacagga aacagcggtg cagcaattgc cgaggaaaag agctgaagat
     6121 agtgagctta gttacctgat gatccagtat gccaatgttc ttttgatgat cgatttttat
     6181 attgcaaaac cagtagtgat tggaatcgat ttagaaagtc tgccgattta tcgtattgct
     6241 tttcaacaat atctgatcag agaactgagg ggaatcggcg gtattgagat tggaagttat
     6301 gaagaaggta aagaatatga tttggtgatc acattttgcc agagaaacaa aaaacagtgt
     6361 gaatattatc tgtcagaatt tgcgtcacct tacgacatta ttcgattgaa aagaagaata
     6421 gagacgctga aaaaagaaaa gaattgatta aaaaatagct tcataataaa aaactgtcaa
     6481 aaaaagaaaa aaagaataaa attttgacag tttctttttt taaaacagta gtatgataag
     6541 agtagttaaa gaaagcgttt gcatataatg gagggaatca caatgacaga atcaacgtat
     6601 atcatggcga ttgaccaagg tacaactagc tcacgagcaa ttatctttga caagaaagga
     6661 agacacatcg gcagttctca aaaagagttc acgcaatatt tcccgcaaga aagttgggtg
     6721 gaacatgatg caaatgaaat ctggaattct gtacagtcag ttattgccgg agcgttcatc
     6781 gaatcaggga tcaaaccaaa tcagattgct gggattggca taacgaacca gcgtgaaaca
     6841 acagtaatat gggaaaaaga tacaggacgt ccaatctatc atgcagttgt ttggcaatcc
     6901 agacagtcag ctggaattgc agataactta aaaaatgaag gctatcaaga atttttccac
     6961 gaaaaaacag ggttagtgat cgatgcctat ttctcagcaa cgaagatacg ctggattctt
     7021 gatcatgtag aaggcgcgca agaacgagct gaaaaaggcg aattgatgtt tggaacgatc
     7081 gattcttggt tagtatggaa actgaccgat ggacaagcac atgttactga ttactcgaat
     7141 gccagtcgga cgatgttgtt caatatccat cagctagatt gggatcagga gattttagat
     7201 ttactaaata ttcctcgagt gatgttacca aaagtcactt ctaactcaga agtttatggt
     7261 tatacgcaag gttatcattt ttatggaagc gaagcaccaa tttcagggat ggcaggagac
     7321 cagcaagccg cattattcgg acaaatggca ttcgaaccag gcatggtcaa gaatacttat
     7381 ggtacaggtt catttattgt catgaatacc ggtgaaaaac cgcaattatc caaaaataat
     7441 ttattgacga caattggtta cggcataaat ggaaaggttt actatgcttt agaaggaagt
     7501 atttttgtgg ctggttcttc gattcaatgg ctgagagatg gacttcaaat gttgcaaaaa
     7561 gctagcgatt cagaagacgc agcgaaacgc tctacaagcg aagatgaggt ttatgttgtt
     7621 cctgcattcg ttgggctagg tgctccttat tgggatcagg cagcacgagg ggctatgttt
     7681 ggcttgacac gtggtacgac aaaagaagat atcattaaag ctactttaca gtcgattgct
     7741 tatcaagtga gagatatcat cgatacgatg caagatgaca ctggaatcaa gattcctgta
     7801 ttgaaagtag atggcggtgc agcaaacaat gaatatctga tgcaattcca aacagatatc
     7861 ttgaatgtac cgatccaacg agcagaaaac ttggaaacaa cggcgctagg ggcagcattt
     7921 ttagcaggat tagctgtcgg ttattggaaa gatacagacg agatccgcga attttatgaa
     7981 gctggcaagc tatttgaagt tcaaatggag gaagagcgcc gagaaaaact ttacaacggt
     8041 tggaaaaaag cagtaaaagc tacccaagca tttgaataag gaggaaatcc atatgttttc
     8101 aaacaagaca agacaagata gcattcaaaa aatgcagcaa gaagaattgg atctgttgat
     8161 catcggtggc ggaatcactg gtgccggtgt ggcagtccag tcagcagcat caggaatcaa
     8221 aacaggattg atcgaaatgc aggattttgc agaagggact tcctctcgct cgaccaaact
     8281 tgttcatggc ggtattcgtt atctgaagac atttgatgtg gaagtagtag ctgacacagt
     8341 tggtgaacgt gcagtcgtac aaagtattgc cccacacatc ccaaaaccag atccaatgct
     8401 tttgccaatc tatgagggcg aaggagcaac aaccttcaat atgttctcgg tcaaagtagc
     8461 aatggacctt tatgacaaac tggcaaatgt gacaggaacc aaatatgaga attatacgct
     8521 gacaccagaa gaagtattgg aaagagaacc gtttttgaaa aaagaagggc taaaaggcgc
     8581 gggtgtctat ctagatttcc gcaacaatga tgcccgttta gtgattgata atatcaaaaa
     8641 ggctgccgaa gatggggctt atctagtaag taaaatgaaa gcggttggct ttttatatga
     8701 tggcgatcaa atcgttggcg tcaaagctcg tgacctgctg acagatgaag tgatcgaaat
     8761 caaagcaaaa ttagtgatca atacgagtgg tccttgggta gataaagtac gaaacttgaa
     8821 ttttacgcgt ccagtctctc ctaaaatgcg tccaactaaa gggatccatt tagttgtaga
     8881 tgcgaaaaaa ctgcctgtac cgcaacccac atacttcgat acaggaaagc aagatgggcg
     8941 gatggttttt gctattccaa gagaaaacaa gacttacttt ggtacgacag atacggatta
     9001 ccaaggagat tttacggatc cgaaagtcac acaagaagat gtagattatc tattggatgt
     9061 gatcaatcat cgctatccag aagcaaatat cacattgaat gatatcgaag caagctgggc
     9121 aggacttcgt ccactgttga ttggtaattc tggttctgat tacaatggtg gagataatgg
     9181 atcgatttca gacaagagct tcaataaagt tgtcgataca gtaagtgaat ataaggaaaa
     9241 caaagtttct cgtgctgaag tagaagacgt gttgaaccat ttggaaaaca gccgtgatga
     9301 aaaagcacct tctacgattt ccagaggtag ttctttagaa agagaaccag atggtttgct
     9361 gactttatct ggtgggaaaa tcaccgatta ccgtaagatg gcagaaggag ctttgcgatt
     9421 gatacgccaa ttattaaaag aagaatacgg aatagagacg aaagaaatcg actctaaaaa
     9481 atatcagatt tcaggtggaa acttcgatcc aactaaatta gaagaaacag tgacagaatt
     9541 agcaaaagaa ggagtagcag ccggtttaga ggaagaagat gctacttata tcgctgattt
     9601 ttacgggact aatgcccgac gtatctttga attagcaaaa gaaatggcac cttatcctgg
     9661 tttgagtctc gctgagtcag ctcggttacg ttatggttta gaagaagaaa tggttttggc
     9721 cccaggcgat tatctcattc gtcgtacaaa tcatctgttg tttgaacgag atcagcttga
     9781 tgagatcaag caacctgtga tcgatgcaat tgctggatat tttgaatgga cagaagagga
     9841 gaaggcgcaa cagactaaac gtttagaagc attgattgca gaatcagatc tgcgggaact
     9901 aaagggggag aaataaatga acagtgatat gactcaaatc atgggggagt ttattgggac
     9961 actgatccta gttttgcttg gagatggtgt gtgtgcggct gtgaatttaa acaaaagtaa
    10021 agcacaggct tctggctgga ttgtcatcgc atttggttgg ggactcgctg taacaatggc
    10081 tgtctatatt tctggattca tgggcccagc tcatttgaat cctgcagtct ctctagctat
    10141 ggcgatgaca ggggcaatta gttggaatct tgtcgttcct ttcatcattg cgcaagtttt
    10201 aggagctttt gcaggggcca ttcttgtttg gctttcttat ttgccacatt ggaatgcaac
    10261 gaaagatgaa agcgcgattt taggaacgtt tgcaacaggt cctgctatac ggaattatcc
    10321 agcaaatgtg atcacagaat taattggtac atttgtatta gttcttgggt tgctagcttt
    10381 tggacaaaac gaatttgcac ctggaacgaa cgtattcgct gtaggtggat tgatcttagc
    10441 catcggtctt tcattaggag ggccaacagg atatgcaatc aaccctgctc gggactttgg
    10501 tcctcgatta gcacatgcgg tattgccaat agctaataaa ggaacttcag attgggcata
    10561 ttcatgggta ccaatcgcag gtccaatgat cggagcaatt atcgcggtag gtgtctattc
    10621 attaatggtg taaatctata aaacatacta aagtttgaca accaaaatgt gtcttgctat
    10681 agttaagtgg aaaataaata agacctagaa gaatggggta tgttttgtga gtgtagctgg
    10741 tatctaatca gtaagtcgaa tagccaataa acaaagttta tttggtgtgc ttatgaaacg
    10801 gtacctgtca ggaaaacaat atctttgtat tctaataagg ataggacatg acttttttaa
    10861 gctgtgttct atccttttat ttatctctag gcccttattt attttgggac aggtatctct
    10921 tcaagaaaag gagagaatat ggacaacgaa atgattagtc aattacaatt tgatcggatc
    10981 aaaaaggaaa tccaagcaag agcaattgga aactatagca aaaaacgaat cagcgatatg
    11041 acggtctcga caaacctaca aactgtcttg gatcgtcaag aagaaacaag agaagcacgc
    11101 ttgatcttag aaagcagtca gcatgttcca tttatggggt tgccgaggat cgatgcactg
    11161 acagaacaag tcaaaaaagg gttgattctg cagccaaccg atttgatcga atatgcggat
    11221 tttcttagaa gcagtcggat gatcacaaag ttttttgata agaatcaata ccagacaccc
    11281 ttgttgtttg cttatagcaa acatcttcct gatttgataa atgtagaaga gttgattgac
    11341 cagaaaataa aaaataataa agtcagtgac gacgcgtcac gtaatttgcg taaagtacgc
    11401 aaacaattac aactaatcga aaaagaaatc cagtcaaaat tactgaaatt cttgcgtcat
    11461 ccgaaaaaca aagaaatgat ccaagaagca atgattgttc aaaaaggcga atactatacg
    11521 attcctatca aagccagcta taaaaataaa atagatggaa cgattatcga tgaatcaaat
    11581 aaaagaacga cggtttttat tgaaccaact gtgatttcta aattgaatga acactatcag
    11641 ttgttaaaag ctgaagaaat cagtgaagaa tatcaagtcc tggcggcatt gactggtgct
    11701 atcgcagaaa acgaagaagt gatcgattta ttgatcgaaa cgatcacggt gttagatatc
    11761 atctttgctc gggcaaaatt tagccgagaa atcaatggaa gcacacctag aatcaataaa
    11821 tcggaacata tcattatcaa gcaaggcaga catccatttt tacctgagca tgcggtacca
    11881 ttggatgttg aaatcggtaa agactatcga ggcttgatta tcaccggagc aaatgccgga
    11941 ggcaaaacag tcgtgttgaa gacagtcggt ttattgactt tgatggcgat gttcggtatg
    12001 caagttccgg caaaagaagg gacagagctt gctgtctttg atgagatttt cgtcgatgtc
    12061 ggtgaccacc aaaatttaga aaatgcgttg agtacatttt ctggtcatat gcaaaacatc
    12121 gctgctattt tgaaaaagat aaaaaggaat acgttggtac ttttagatga gattggcagt
    12181 ggaacagagc caaatgaagg ggctgcacta gcgatcgcaa tcatggaatc tatgtatgag
    12241 caaggggcat tgatcattgc cacgacccat tatggagaaa tcaagaaatt cgccggagat
    12301 catgaagatt ttgtgccggc tgctatggct ttcgatcggg aagcacttcg tcctaaatat
    12361 cagttgcggg ttggagaaac aggagaaagc caagctttgt ggattgctca taaaatggat
    12421 atgtcgatga aattgatcca tcaagcagag cgttacttgg cggaaaaagc ctatcggaca
    12481 cagaaaaaag aatttcttcc taagacagca gttacaaata ggaaggagaa taaaaaagaa
    12541 cgccagctct ttgcaaaagg tgatcggata tttgtcaatg aataccaaaa agaagcgtta
    12601 gtttatgaag atataggtga agatacgatt gatgtatatt tagacaaaaa aataatccac
    12661 gtgcctcgtc agcgtgttcg tctggtgcgt tcagcagaag atctgtaccc gacaggttac
    12721 gatttagaca gtctgtttat cgattataaa acacgtaaac ggcagcgtga cctagagcga
    12781 ggatcaaaaa aagctcataa agtactagta aaagaaatgc gcaaacgaca ggaagaaaga
    12841 agagtaaacg atgaaaacag caagtgatta tctcaaatta gctcataata tcagtggctg
    12901 taaagaaaaa taaaaacagt gaaatcattg gttttattcc ccgtgtaagc taaaataaaa
    12961 agaaaagagt gctgaggagg tagggcgata aaatgaaatt aagcacagac tggcgtaaag
    13021 aaatacaaac gattccgaat cttttatcga tattccgtat ttttttgctg ccgatttatc
    13081 tttattttgt gctgagacag tcattttata tagcaggagc ggtcattgtt gtctcaggtt
    13141 tgtctgatta tctagacggg gtcattgctc ggagatataa tcaggtaaca gacttaggga
    13201 aggtgcttga tccgtttgca gataaattga cacaactttt tctgattttg tctatggcat
    13261 ggtatcgacc gtggctttgg ctgttgtttg gtctattctt gataaaagaa ggctttatgt
    13321 ttgtagctgg attaatcggg ctatctaaaa atatcaaatt aagtggagcg aagtggtatg
    13381 gcaaagtagc gactgctgtc atctatgttg gcatgatcct tttgctgctt ttcccagaat
    13441 taccaacgct gtgggtgaga gtcatttttg ccgtcatcac ttacggtctt ttacaatctt
    13501 ttgtcttgta tgctgtcgag taccgaaaga tgtttcaaag aaaataaaaa aggggtgtga
    13561 cctatgtcgc attccttttt ttgataaata gactcccagt agctacttct cgataataag
    13621 ccaaccaaac caaaaacagg agaagttgtt tttgaaatta tttcttgtga ttcggagtta
    13681 aatggtaggc tcacaacctc ttggtaaacg gtgttcaatc agctgacctt cggtattagc
    13741 tcaaaaaacg gcaaaatatg aggagc
//
LOCUS       DACEXQ010000050        13713 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.050, whole genome shotgun sequence.
ACCESSION   DACEXQ010000050 DACEXQ010000000
VERSION     DACEXQ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.050"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            140..382
                     /gene="yaaA"
                     /locus_tag="IXH04_001090"
     CDS             140..382
                     /gene="yaaA"
                     /locus_tag="IXH04_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAQ1851710.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            369..1493
                     /gene="recF"
                     /locus_tag="IXH04_001091"
     CDS             369..1493
                     /gene="recF"
                     /locus_tag="IXH04_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAQ1851711.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1490..3436
                     /gene="gyrB"
                     /locus_tag="IXH04_001092"
     CDS             1490..3436
                     /gene="gyrB"
                     /locus_tag="IXH04_001092"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAQ1851712.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3456..5927
                     /gene="gyrA"
                     /locus_tag="IXH04_001093"
     CDS             3456..5927
                     /gene="gyrA"
                     /locus_tag="IXH04_001093"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAQ1851713.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6163..6462
                     /locus_tag="IXH04_001094"
     CDS             6163..6462
                     /locus_tag="IXH04_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAQ1851714.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6512..7024
                     /gene="ssb"
                     /locus_tag="IXH04_001095"
     CDS             6512..7024
                     /gene="ssb"
                     /locus_tag="IXH04_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ1851715.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            7050..7286
                     /gene="rpsR"
                     /locus_tag="IXH04_001096"
     CDS             7050..7286
                     /gene="rpsR"
                     /locus_tag="IXH04_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAQ1851716.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7429..9402
                     /locus_tag="IXH04_001097"
     CDS             7429..9402
                     /locus_tag="IXH04_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAQ1851717.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9408..9860
                     /gene="rplI"
                     /locus_tag="IXH04_001098"
     CDS             9408..9860
                     /gene="rplI"
                     /locus_tag="IXH04_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAQ1851718.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            10134..11501
                     /gene="dnaB"
                     /locus_tag="IXH04_001099"
     CDS             10134..11501
                     /gene="dnaB"
                     /locus_tag="IXH04_001099"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAQ1851719.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            12281..13573
                     /locus_tag="IXH04_001100"
     CDS             12281..13573
                     /locus_tag="IXH04_001100"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAQ1851720.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT         4568 a         2474 c         3009 g         3662 t
ORIGIN      
        1 ctttctgttt atttttgttt aaatcacctt ttttagatta aatcgtctta tatggaaaaa
       61 tatatggata aatttgtttt attgcgaaaa aaaaggtata atagagtata cttttaataa
      121 tgaaaatgag ggatgggcat tgaaacaaca agtcattcta aatacggaat tcatgacgtt
      181 gggccaaatg ttgaaagaag tcacagttat cggtagtggc ggacaagcaa aatggtattt
      241 ggcagaaaac actgtgttag tcgatggcga accagaaaat cgtcgtggac gcaaacttta
      301 tcccggcatg atggttgaag ttccagaagt aggaactttt tttatggtaa aaaaaggaaa
      361 cgacgataat gaggctgaat aagctgtatt taaaaaatta tcgaaattac gaagagctga
      421 acatcgaatt ttccaagaac ctcgtcatct ttttaggaga aaatgcgcaa ggaaaaacga
      481 atcttttgga aagtatctac gtattagcta tgacgcgaag tcatcggaca agtagtgagc
      541 aggaattgat tggctggact gacgatcagg cgatgattca aggagagatc acaaaaggtt
      601 catccactct tccattagaa atcttattat ctaaaaaagg ccgaaaaacc aagatcaacc
      661 atatcgaaca gaaaaagtta agtacttacg tgggacaaat gaatgtcatt ttatttgcac
      721 cagaagatct ttctttagta aaaggcagcc cgcagaccag gcggaagttt ttagatatgg
      781 aattaggcca gatcaatcca atctatcttt atgatcttgt ccaataccaa gcaattttga
      841 aacaaagaaa ccagtacttg aagcaattga atgaaaaaaa acaaactgat ttgctttatt
      901 tggatatttt gtccgaacaa ctagcagcgt ttggcagcaa agtattgaaa gcaagagcac
      961 agttcgtcaa gcgattggag ttttgggcta attctttgca tcagcaaatc acccatcaaa
     1021 aagagcagct ggaaatcgag tatctcaccg cagtcgattc tttagaaaca catacgcaag
     1081 agcaaatcca agaacaattt ctcgcacttt tgaaccaaaa taaaaagaaa gacctttttc
     1141 ggggaacaac gacagttggt cctcatcgtg acgatctttc cttttttatt aatcagaaaa
     1201 atgtgcagac ttatggatcg caaggacagc aaagaacaac tgcactaagt gtcaaattag
     1261 cagaaatcga tttgattaaa gaagagacag gggaatatcc aatccttttg ttagatgatg
     1321 tgatgagcga gctggatgat aatcgtcaac tccatctgct agaaacaatc gaaggaaaag
     1381 ttcaaacgtt cttaacaaca acgacacttg accatgtaaa aaataaaatg actgttgaac
     1441 ctgagatttt ttatgttcaa caagggaaac taaaaggaga agaaactgaa tgacagaaga
     1501 aagaagttta gtagaacgcg ctaaagagta tgatgccagt cagattcaag tacttgaagg
     1561 acttgaagcc gttcgtaaac gacctggcat gtatatcggt tcgactagtt ctgagggtct
     1621 gcatcacctt gtttgggaaa ttgtcgataa ttcgatcgat gaagtattag caggttttgc
     1681 aacgaaaatc catgtgatta ttgaaaaaga caacagtatc accgttattg acgacggtcg
     1741 ggggatacct gtagatatcc aggcaaaaac tggacgtcct gcagttgaaa cggtctttac
     1801 tgttttacat gctggaggaa aattcggcgg tggtgggtac aaagtttctg gtggacttca
     1861 cggagtagga tcttctgttg ttaacgcgct ttctacacag cttgatgtca aagtatacaa
     1921 agaaggaaat gtgtactatc aagaatatcg tcgtggagct gtagtggacg acttaaagat
     1981 tatcgaacaa acggatcgcc atggaacaac ggttcacttt acgccagacc ctgaaatttt
     2041 tacagagaca actgagtttg atttttcaaa attggctacc cggatccgtg aacttgcttt
     2101 cttgaaccgc gggatgcgta tatcgattga agacaaacga gaagaagaac cagtcatcaa
     2161 tgaataccat tatgacggcg ggattaaaag ttatgtcgaa tacttgaatg caaacaaaac
     2221 cgtgattttt ccagaacctg tatatttgga aggtgaacag caagatatcg cagttgaagt
     2281 ctctatgcaa tatacagacg gctaccattc caatatcatg agttttgcca acaatatcca
     2341 tacgtatgaa ggcggaacac atgaatcagg attcaagact gctttaactc gtgtgataaa
     2401 tgattatgcc cgaaaacaaa agctgatgaa agaaaatgac gacaacttga ccggtgaaga
     2461 cgtgcgagaa ggattgacag ctgttatttc tatcaagcat cctgaccctc aattcgaagg
     2521 acaaacaaaa acaaaattgg gcaattccga agtacggaca gtcactgatc ggttatttag
     2581 cgaacatttc atgaaattct tgctggaaaa cccaagtgtg ggccgtcaaa ttgttgaaaa
     2641 aggattacta gcttccagag ctcgtctagc agcgaaacgt gcgcgtgaag taacgagacg
     2701 taaaggtgct ttagagatta gtaatctgcc aggaaaatta gcggactgct ctagcaacga
     2761 tcctgaaaaa tgcgaattgt tcatcgtcga aggagattcg gccggcggat cagctaagca
     2821 aggtcgtaat cgagagtttc aagccatctt gcctattcgt gggaaaatct tgaatgttga
     2881 aaaagcaagc atggacaaga tcttggcaaa tgaagagatc cggtctttat ttactgctat
     2941 gggtacaggg tttggagaag attttgatgt atcgaaagct cgctaccaca aattagttat
     3001 catgactgat gccgatgtcg acggcgcgca tatccgtaca ttgctgctga ctctttttta
     3061 tcgttacatg cgtcctattg ttgaagctgg atacgtctat attgctcagc cacctttata
     3121 cggggtaaaa caagggaaga atattacgta tgtacaacca ggaaaaaatg cagaagaaga
     3181 gttgaaacaa gtcgttgctt cacttcctgc ctcaccaaaa ccaagcgttc agcgttataa
     3241 aggacttgga gaaatggatg atcatcaact atgggaaaca actatggacc caagcaatcg
     3301 gatgatggcc cgcgtcagtg tggacgatgc tattgcagca gatcagatat ttgaaatgtt
     3361 gatgggggat cgagtagaac cccgtcgtgc attcatcgag gaaaatgcac actatgtgaa
     3421 gaacttagat atttaatcat gaaggaggaa aaagaatgag tgaagaaatc agagaaaaca
     3481 tccatgatgt caatctaacc agcgaaatga aagactcctt catcgattat gcgatgagtg
     3541 ttattgtcgc acgtgcactt cctgatgtca gagatggtct gaaacccgtt catcgccgta
     3601 ttttatacgg catgaacgaa ttaggtgtaa caccggataa agcccataaa aaatctgcca
     3661 gaatcgtcgg agatgtcatg ggtaaatacc atcctcatgg agactatgcg atctatgagt
     3721 caatggtacg gatggcacag ccattcagct accgttatat gctagtggat ggtcatggaa
     3781 actttggttc tgttgacggt gacggtgccg ccgctatgcg ttataccgaa gcacggatga
     3841 gcaaaatcgc tacagaaatg ctgcgtgata tcaataagaa cactgttgat ttccaaagca
     3901 actatgatga tacagaaaaa gaaccagttg tactacctgc tcgcttcccg aatcttttag
     3961 tgaacggaac aacggggatc gccgtaggta tggccacgaa tatcccacct cataacttgt
     4021 ctgaggtagt agcggctatc gatttgctga tggataatcc tgatgtgacg accaatgaat
     4081 tgatggaagt attacctgga ccagattttc caacaggtgg tttggttatg ggaaaatcag
     4141 gaatccgaag agcgtatgaa acaggtaagg gttctattac cgtacgtgca aaagttgaga
     4201 ttactgagat gccaaatggt aaagaacgga tcatcgtgac agaattgcct tatatggtca
     4261 ataaagcaaa attgatcgaa cggatttctg aactgcacag agacaagcgt atcgaaggga
     4321 tcacagattt gcgggacgaa tcttcccgtg aaggtatgcg gatcgtcatt gacatccgtc
     4381 gtgacgccag cgcatctgtt atcttgaata acttatataa attgacatcc ttgcaaaatt
     4441 catttggatt caatatgttg gcgatcgaaa aaggtgtgcc gaaagttttg agcttgaaac
     4501 aaattcttga aaactatatc gaacatcagc gcgaagtcat cacaagacgg acagtctttg
     4561 aaaaagaaaa agcagaagca agggcacaca ttttagaagg tttgcgtatt gctttggatc
     4621 atatcgatga aatcattgcg attatccgcg ggtctaaatc agatgacgaa gcaaaagcga
     4681 cgatgatcga acggttcgat ttatccgatc gccagtctca agctatttta gatatgcgtt
     4741 tacgccgatt gaccggtttg gaacgggaaa aaatcgaaaa tgaataccaa gaactcttaa
     4801 aactgattga agatttaact gacattctgg caagaccaga acgtgttaca gaaatcatca
     4861 agaatgaatt agcagacttg aatcaacgat ttggtgataa acgacgcaca gaattactag
     4921 tcggtgaggt cttaagtctg gaagatgaag atttaatcga agaagaagaa atcgttatta
     4981 cattgacaaa caatggttac atcaaacgtt tggcaaataa tgaattcaga gctcaacgaa
     5041 ggggtggacg tggtgtccaa ggaatgggta tccacaatga cgacttcgtc aaaaatctgg
     5101 tttcttgttc tacccatgat acattgttat tctttacgaa taacggaaaa gtgtatcggg
     5161 caaaaggata cgaaattcca gaatacgggc gcacagccaa aggaatacca attatcaatt
     5221 tactaggtat cgattcgagt gaaagcatcc aggcaatcat tgcggtgacg ggagaagcag
     5281 aagaaggcca ctatctcttc tttacgacgc gtcaaggtac agtaaaacgt acttctgtca
     5341 ctgcttttgc taacatccga agtaatggtc tgatcgcaat tggtttgaaa gaagacgatg
     5401 agctcgtcaa tgtgctactg acagatggtc aatctaatat cattattgga acacataacg
     5461 gctactctgt cactttcaaa gaagaagcag tccgcgatat gggacgtaca gcttccgggg
     5521 ttagaggaat acgcctgcgt gaggaagatt atgtcgttgg ggcagcctta atgaaggaag
     5581 atcaagaagt attagtgatc acagaaaaag gttacggaaa acggacaaaa gtatcggaat
     5641 atccagtgaa aggccgcgga ggtaaaggga tcaaaacggc gaacatcact gaaaaaaatg
     5701 gtccactagc tggattgacc actgtttctg gagatgaaga catcatgctg attacagata
     5761 aaggtgtcat tatccgtttc aacgtatcga ctgtttctca aactggacgt tcaacattag
     5821 gcgtacgttt gatgaagatg gaagctgata cgaaagttgt cacgatggca gcagtcgaac
     5881 cagaagctgc agaacaaaca gttgaagaac aaggatcaga agaatagcca tcaagactaa
     5941 ttgtgtaagc tagcaagaat gaccgattaa aaaatctggt tattcctgct ggcttttttg
     6001 attagttgaa tttctcttaa aatattcgaa actgttcatt ctgtattgct tttgaccaaa
     6061 atagagtgta taatcagagc ttgtgagtag tctaattaat tagataactc tccttgctct
     6121 taggaaccta agagccgaaa gtccacaggg aggtgaatat caatggaaaa cacgaaatat
     6181 gaaatcatgt acattattcg tccaaacatt gatgaagaag caaaaactgc tttaatcgaa
     6241 cgtttcgatg caatcttgaa agataacgga gcagaagtta tcgaatcaaa agattgggaa
     6301 aaacgccgtt tcgcttatga aatgaacggt taccgtgaag gtatctatca catcgttaaa
     6361 gtatcttctc catcatcagc aaatgctgtc aatgaatttg accgtcttgc taaaatcaac
     6421 gacgatatca ttcgtcacat gattgttaaa gaagaagctt aattgttgtt tcatgtgaaa
     6481 caatagaaaa cgatgagagg agatgactat tttgattaat aatgttgtat tagttggacg
     6541 tctgacgaaa gatccagatt tgcgttacac agcaagtggt acggcagtcg caacatttac
     6601 tttggctgtg aatcgtaatt ttacaaacca aagcggcaat cgcgaggctg attttatcaa
     6661 ctgtgtgatt tggcgcaaat cagcagaaac tttagcaaac tatgctcgta aaggaacact
     6721 tttaggtgtt actggacgta tccaaactcg ttcttatgat aatcagcaag gacaacgtgt
     6781 atacgtgaca gaagtagtag ctgacaactt ccaattgctg gaaagtcgtt ctgcatctga
     6841 aaatcgccaa caaagcggcg gattccaaag ttctggtcaa tcagctggcg gatttggcgg
     6901 aaacaataac tctaatcaaa catctcaatc atccaacggt atgcctgatt tcgaccgtga
     6961 tacttctgac ccattcggct caagttcaac gatcgacatt tcagacgatg atttaccatt
     7021 ctaaacgaat ttgataggag ggaaatagaa tggcacaaca aagaagaggc ggacgtaaac
     7081 gtcgtaaagt agactacatt gctgctaacc atattgaata tatcgattat aaagatatcg
     7141 aattactaaa aagatttatt tcagaacgcg gtaaaatctt gccacgtcgt gtaactggta
     7201 caggcgctaa aaaccaacgt aaaattactg ttgcaatcaa acgcgctcgt atcatgggct
     7261 tgctaccttt cgttagcgaa gactaataaa tcctaggaat tgaatgactg atccttttga
     7321 tcagctattc aattcctttt tttctgtaaa aataacctct attcgtttag tcgctcgaat
     7381 gacgaaagtt atggtaaaat caaattagtc atatatggga ggaaaattat ggaaaataaa
     7441 aacaatcatt tatccccgaa acctctcttc cttatcctgt ttatattaat cgaagtattg
     7501 gtagtcttaa ttttaccatt tcgttttgtt agcgcttcgg tcattctatt gatgaattta
     7561 tggggtgttc gctattggtt gaaaatgaaa cgttggctta ttttaaataa acgtgaaaaa
     7621 atccagcagg cttcgactgt agctgaaacc aatctagctt atgtctcaga agtcatgcct
     7681 gtaggagtga ttgcttatca gccatcaagc caaaaaatcg aatggctcaa tccattagca
     7741 ttgaaaatca aagatcaaag tggattaaca gaggaagaat tgctagatac tttttttact
     7801 gcaaaagaaa aaaagaaaaa ccaagtctat ctagcagata aaacatttca gtttgatatg
     7861 gatacagtaa agggtgttgt ttattttaat gatattaccc aaaaccagtt gttaaaactc
     7921 catcagacga acagtcagcc ggtgattggt attttatcta ttgataacta tgcagatgcc
     7981 attgataaat tggatgacaa agagatttct tatttgaact ctttcgtcac tacactagtt
     8041 tctgactgga tgaatgaata ccatgtcttt ttcaaaagaa tcaacgcaga acggtttttc
     8101 ttcgtagcac agatgatcga tctgaaaaag atgatggata aaaaatttga tttgctggat
     8161 cgctttcgag cagaagctga aaaacagagc tcgcctatca cccttagtat ggggattgct
     8221 tatggctcaa gaaccctcga tgaaatcggt ggagaagctc agaataattt agatgttgct
     8281 ttagtccgtg gaggcgacca agttgtcgtg aaagaggtaa aagaaggagc aaaacctgtt
     8341 tattatgggg ggaaatctgc taagaatgtc aaacgcacgc gtgttcgttc tcgagcgatg
     8401 agtaccgctt tgcgtcggat ttttgatgaa acaggcgatg tgtttatcat ggggcacaag
     8461 tttccggata tggatgcttt aggctctgct tttggtgtca gttgtctggc acgcttcaac
     8521 cagaaaaaag cttacatcgt tatcaacgag aacgagatca ttccagatgt agaacggtgt
     8581 cttgaagaaa tccataagca tcctgaatta gaaatgcaac taatctctcc agaacgtgcc
     8641 atagagctga aaaaagaaga ggacctcctc gtgatggtgg attaccataa accttctcta
     8701 tcgatctctc agggcctata tgagcaattt gataagattg tgatcattga tcaccatcgt
     8761 cgtggggaag agtttccaag taaacctctg ctgacttata tcgaatcttc agcttcttca
     8821 gcttcagagc tagtttctga gctgatccaa taccaaagca gcaaaacaaa tcgcttagat
     8881 cgttttgagg ccacattatt gcttgcaggg atggttgtag atacaaatag tttcaatgtc
     8941 cgtacaactg cacgaacttt tgacgttgcc agctatttaa ggtcttgtgg agcagacgca
     9001 tccttagtac aatatttatt gagctcggat ttagcgacat acttggagat cagcaatttg
     9061 attgctggaa gcgactatgt gacttctgat attgttgtgg cggcagggac agacgaaaaa
     9121 gagtataata gtgttatagc agcaaaaaca gcagatacat tgctttcgat gatcaatatc
     9181 aatgcagctt tcgtcattac aaagcggaca gacggcttga ttgggatcag tgccagaagt
     9241 accggcacga tcaacgtcca aatcatcatg gaatccttag gtggcggagg acacttcaca
     9301 aacgcagcta ctcaactgga aaatacgaca gttgaggaaa caaaacagaa gctatttgct
     9361 gcaatcaaag aaaatatgaa tcaaatatat gataaggagt aatccaaatg aaagtaattt
     9421 ttttacaaga tgtaaaagga aaaggtaaaa aaggggacat caaagaagtt ccagcaggtt
     9481 atgcacaaaa ttatctaatc aaaaataact tggcaaaaga agcaacgaaa agcagtatca
     9541 gtcagttagc aggacaaaag aaagcacaag aaaaacacga agctgaaatc ttggaagaag
     9601 ctaaaaagtt aaaagaattc ttggaaaaag aagatactgt agtcgaattg aaggcgaaag
     9661 caggggaaga cagccgtttg tttggttcga tcccttcaaa acaaattgct gaagggttaa
     9721 acaaacaata caaaatcaaa ttagataaac gaaaaatcga attagaaaat ccgatccgtt
     9781 cactaggcta cacaaacgta cctgttaagc ttcatcatga ggtgacggct acgatcaaag
     9841 ttcatgtcgt acaagaataa caaatcttga tagcgatgtt cagctggtaa gtataaccag
     9901 aaggaaaaaa gacaaaccga aatttatctg agaaaacagc tgcagcaatg aaagcaaaag
     9961 ctgcaaatcg atttcagatt gatttcggtt tcttttttgg gaacagcaaa atagcataaa
    10021 gaaaacgagt ataataaaag agaactattt ttttattaca ctttcggtac gaatgatgac
    10081 ttttctctca ttttcttgta aaatgaagca attgaagaca aggagcgata cgtatgaatg
    10141 aagtgtggca ggatcgaatc ccaccgcaaa atattgaagc tgaacaagcg gttttaggct
    10201 ctgtttttct tgacgcagaa acgatcattg atgccatgga gtacatagag ccaaaagact
    10261 tttatcgtcg tggccatcaa attatttttg agacaatgat cgagttaaat gatcgtaatg
    10321 aagcgattga cgtggttact gtcaaagacc ggctggaaca agccaatttg ttagaagatg
    10381 ccggtggttt aagttatctc tcagacttgg cattagcagt tccgacagca gcaaatatcg
    10441 tctactatgc gaaaatcgtt gaagaaaaat cattgctgcg tacattgatc cagacagcaa
    10501 caggaatcgt tacgaaagga ttcgagcaag gagaagaagt tcaaacaatt ttagatgatg
    10561 ccgaacgcag tatcttggag gtttcggaaa aaagaaatcg aagcggcttt ttatctattg
    10621 ctgaagtact gaactccacg attgaaaata tcgatcgact agctcaaaac aatgaagaaa
    10681 tcacaggtct accaactggc tatcaaggtc tggataaaat gacagcaggt ttccagcccg
    10741 aagaactaat cattcttgct gcacgtccag cggtaggtaa aacagcgttt gcattgaata
    10801 ttgctcaaaa tgtaggaaca aaaacagatc gttctgttgc gatatttagt ttggaaatgg
    10861 gggcagaatc tcttgtaaat cggatgcttt gtgcagaagg ttcgattgag gccagccatt
    10921 tacgtacagg tcagctttcg gaagaagaat ggtcgaatct gatcgttgcg atggggagtc
    10981 tttctcgagc aaatattttt atcgatgata caccagggat caaaatctct gaaatccgtg
    11041 cgaaatgtcg gaaactagcg caagaaaaag gcaatctagg cttgatattg atcgattatt
    11101 tgcagttgat tgaaggtaac ggtaaagaga accgtcaaca agaagtatca gatatctctc
    11161 gccaactgaa aaaactggcg aaagagctga aagtacctgt cattgctctt tcacagttat
    11221 ctcgtggagt agaacagcga caagacaaac gaccagtgct aagtgatatt cgtgaatccg
    11281 gatcaattga acaagatgcg gacatcgtag ccttcttgta tcgagatgat tattatgaaa
    11341 gagaaggcgg agatgacgat gatggaaacg agccaaaaaa tgacaacgtc atcgaagtca
    11401 tcatcgagaa gaaccgaagc ggtgcacgag gaacagttga actactgttt atcaaagaat
    11461 acaataagtt ttcgtcgctt tcgccaagag aagaatttta aagaagaggt tgtgagcctt
    11521 gagcaatccc gagagctctc gtaagaagat gcaaggagta accgcaggaa cgactagagt
    11581 acttaattta aacagatctc caaagggatt gtgacaaaag tcttgtcaca gtcccttttt
    11641 ccgaataaac ggtgttcaat cagctgaccc ttggtattag ctcagcaaac gtcaaaatat
    11701 gaggaactat tttgacgttt gtttctctaa tactcaggtc aaaacgctga tcttacgacc
    11761 tcttttcctt aaacggtgtt ctcctgccaa ctcctcggtc acaagtcaca atatcatcaa
    11821 acatgagaag cagttttttg aaattgctcc ttgtgactca gagttaaacg gcaggctcac
    11881 aacctctttc ttaaacggtg ttcaaaagct tgcatctgcg gtaatacgcc caatcaaacg
    11941 aaaaatatga gaagctattt ttgtttggct gttcttatta cttgatgcag gacagctttt
    12001 tcacaacctc tgattcacgg tgttccaaaa gtagcttctt cgaaaaataa gtcgaaattt
    12061 cacaaaaatt tgagaagcaa ttttcgtaca ttccttctta tttcttgaag ctgaccactt
    12121 ctgttacaac ctctttttta ataaccattc taaaaagata aagttcgttt tttaagcgtt
    12181 tttataaaat aaaaataaaa atcattcgat tttatttgaa atatgtatct attattgata
    12241 caatggttca ggattaaata atatagaacg aggtgttcac atgtcatctg tagtagtagt
    12301 aggtacgcaa tggggcgatg aagggaaagg aaaaatcaca gactttctca gtgaaaatgc
    12361 tgaagtgatt gcacggtacc aaggtggcga caatgcaggg cacacaatta agtttaacgg
    12421 tgtgacttat aaactgcatt tgattccttc tgggattttt tataaagaca agatcagtgt
    12481 gatcggaaac ggcgttgtag tcaatcctaa atcattggtt aaagaattag cttatttaca
    12541 tgaaaaaggt gtagaaacaa gtaatttgcg tatttctgac cgcgcgcacg taatcttgcc
    12601 ttatcatatc aaattggacc aattacaaga agatgctaag ggcgaaaata agattggtac
    12661 aacgattaaa ggaatcggac ctgcctatat ggataaagca gctcgtgtag gtatccgtat
    12721 tgcagactta ttagataaag aaatttttga agaacgtctt cgtttgaatc tagatgacaa
    12781 aaaccgtcaa tttgtaaaaa tgtttgattc agaaccactt gattttgacg atatttttga
    12841 agaatattat gaatacggac aacaaatcaa acaatacgtc acagatactt ccgttatctt
    12901 gaatgatgct ttggatgcag gcaaacgcgt gttgtttgaa ggcgcacaag gtgtaatgct
    12961 ggatatcgac cagggaactt atccttttgt tacttcttct aacccagtag ccggtggggt
    13021 aacaatcgga agcggtgtag ggccttcaaa aatcaataaa gttgtcggcg tctgcaaagc
    13081 ctatacgtca cgtgtcggtg atggaccatt tccaactgaa ttatttgatg aaacaggaga
    13141 acaaatccgc gaagtaggac gagaatacgg tacgacaact ggtcgtccgc gtcgtgtagg
    13201 ctggtttgat tctgtagtta tgcgccattc taaacgagtt tcaggtatta ccaacctatc
    13261 tttaaactcg atcgatgtat taagtggatt ggaaacagta aaaatttgta cggcttatga
    13321 attagatggc gaactaatct accactatcc agcaagcttg aaagagttga accgctgcaa
    13381 accaatctat gaagaattgc caggctggtc agaagacatt acaggatgca aaacacttgc
    13441 agaactgcca gaaaacgcac gtaactatgt acgtcgtatc tcagagcttg ttggtgtacg
    13501 tatctcaaca ttttctgtcg gacctgatag aacacaaaca aacatcttag aaagcgtctg
    13561 ggcgcaaatc taaggataaa aacgacgact gaatcaggac gttcttacca gagcgagtcg
    13621 aagtgtttat ctatacgata ataataactt gaacactgtt tattcagaat aagggactgt
    13681 gacaaaaacc gtgtcacagt ccctttttcc gaa
//
LOCUS       DACEXQ010000051        13577 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.051, whole genome shotgun sequence.
ACCESSION   DACEXQ010000051 DACEXQ010000000
VERSION     DACEXQ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13577
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.051"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(362..1081)
                     /locus_tag="IXH04_001101"
     CDS             complement(362..1081)
                     /locus_tag="IXH04_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ1851721.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(1130..2776)
                     /locus_tag="IXH04_001102"
     CDS             complement(1130..2776)
                     /locus_tag="IXH04_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ1851722.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            2955..3545
                     /locus_tag="IXH04_001103"
     CDS             2955..3545
                     /locus_tag="IXH04_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1851723.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            3637..5142
                     /locus_tag="IXH04_001104"
     CDS             3637..5142
                     /locus_tag="IXH04_001104"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAQ1851724.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(5226..6578)
                     /gene="celB"
                     /locus_tag="IXH04_001105"
     CDS             complement(5226..6578)
                     /gene="celB"
                     /locus_tag="IXH04_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ1851725.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(6578..7096)
                     /locus_tag="IXH04_001106"
     CDS             complement(6578..7096)
                     /locus_tag="IXH04_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851726.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(7112..7438)
                     /locus_tag="IXH04_001107"
     CDS             complement(7112..7438)
                     /locus_tag="IXH04_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAQ1851727.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(7451..9430)
                     /locus_tag="IXH04_001108"
     CDS             complement(7451..9430)
                     /locus_tag="IXH04_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ1851728.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(9451..9765)
                     /locus_tag="IXH04_001109"
     CDS             complement(9451..9765)
                     /locus_tag="IXH04_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAQ1851729.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            9933..10226
                     /locus_tag="IXH04_001110"
     CDS             9933..10226
                     /locus_tag="IXH04_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAQ1851730.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(10304..11416)
                     /locus_tag="IXH04_001111"
     CDS             complement(10304..11416)
                     /locus_tag="IXH04_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ1851731.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            11803..11982
                     /locus_tag="IXH04_001112"
     CDS             11803..11982
                     /locus_tag="IXH04_001112"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851732.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            12176..12346
                     /locus_tag="IXH04_001113"
     CDS             12176..12346
                     /locus_tag="IXH04_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1851733.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(12770..13510)
                     /locus_tag="IXH04_001114"
     CDS             complement(12770..13510)
                     /locus_tag="IXH04_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1851734.1"
                     /translation="MTKANRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGL
                     TTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTP
                     GGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVET
                     QAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGE
                     TIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT         4217 a         2776 c         2325 g         4259 t
ORIGIN      
        1 tttggcctga gtattagaga aaaaaacggg aaaatagttc ctcatatttt gccgtttttt
       61 gagctaatac cgagggtcag ctgattgaac accgtttatc agaggttgtg aattcagcgt
      121 tttggcctga gtattaggga gatcgtgtcc ttactatcta actctgagtc acaaggaaca
      181 atggggactg taacaaaagt cttgtcacag tcccttattc cgaacaaacg gtgttccaga
      241 agtagcttct acggaaataa gccgaaattt cacaaaaaag ccatccgaaa gtcagacgga
      301 caagtctgct tttcagatgg cgctacaact actctgctta ttcttttagc tatccattca
      361 attatttttc tctcacttgt tctagctctt ctttggtcat gggtcgatat tgccctttat
      421 tcaaatcttt aggcagccaa aattttccca tacggattct ttttaaatat aagacttctt
      481 ttcctactgc tttgaccatt cgcttaactt ggtgaaactt tccttcatgc aaaatcagac
      541 ggataattga agtttctgtc tcttgatcta cttgatcaat ttggagtttt gccggcaatg
      601 tctgctcatc gccatcaata atcagtcctt ctgcaaataa acggatatct tcttctgtca
      661 ttataccgct aacctcagct agatattctt tctccacatg ttttttaggt gatagtaact
      721 gatgggcaaa caccccgtca ttcgttaaaa tcaagaggcc ctctgtgtct ttatctaatc
      781 ggccgactgg aaaaagatct tcccgataat cctggtcttc caataaatcg attacggttt
      841 gatcatgatt atcctgagta gcagaaatga ctcctgcagg tttatgcaaa atgtagtaaa
      901 aatctttttg atacgtaatg atttctccta aataagtaac cgcatcttcg aattcattta
      961 cttgcgcttt atcgcttttc accacttgtc cattgacttc gatcgttcct tttttgatta
     1021 atcctttgac ttctttgcgg ctgccaagtc ccatatctgc taaaaactta tctaaacgca
     1081 tgcgtttccc ccatttcaaa agagcatcaa tctagctctt ctgattcact tattttattt
     1141 ttaatttatg tcgtaaacgc gctattcctg gtcctagtag tctttctgcc aagcggattt
     1201 tcaaagctaa ataaccatac actaaagccc ctatacctgc tactacaaga atcaacaata
     1261 gagactggaa tttgctatct tcgtttaaaa aagcaccaaa aatcatcttt gtgatccacg
     1321 aagccaaaag cataatcaag gtaagaataa agatcagcaa tgtcctgcgc cagacaaaac
     1381 ttggtttaaa gcgggctact tcatggattc tctttaagat caaggtacaa gacacagcaa
     1441 atccgatcat tgtcgccaat aaagggccgt atacttcaaa taaacgaatg gctgggtatt
     1501 gaagaaggag tttcactaga aacccaataa ccaaataatt aattgccgcc ttgttttcaa
     1561 acattccttg cagcatgttg gaaactagca tatatagtcc taggaagagt cctacaaaac
     1621 ttgcttggat cagtacatta cttcctagag aatccggtgt ataaaataat gtatttagcg
     1681 gataggcaag taacataaca ccaaatgtcg ccggaaacat aaaaaatgaa aacaactgta
     1741 aatttccgct tgtcaatttt gccagaccta ctcgatcttt caacgtaaca gcttctgtaa
     1801 tcaacggtaa cccagtagac gcgatcgatg ttgccaatgc aataactacc atcgtcaact
     1861 tatctggatt ggcactaaaa atcgaaaaaa gatccagtaa ctgtgcattg gaatactcgg
     1921 tggatgcaga catgatcttc ataaaagtga actgatcgac taatttgaag atcgttaccc
     1981 ctgaacccac aataataaac ggaatcgctt ctttaacggt atctaacaac aattccttcg
     2041 ttgcaatatt tattttgttt tcgctgtagt gaataaacgc cgatgtatac gcttgatgtt
     2101 tcttcaaaaa atacaaaagg acaagaatac ttgctagcat accgataaat gccgcgaaag
     2161 ttgattgcgt gaccgccgtc acatagtcac catccatgac tttcatgatg atgaatgtag
     2221 ccaatagcat ataaaaaata cgtgccacct gttctacaat ctgcgacagc gcataaggca
     2281 tcatttcttg attgccttgc aaataccctc gtatcacact catacaagga aaaaccaata
     2341 cagcgatact caaagaacgc atcgttggaa tcagttcttc tccgccacca gaagcatgag
     2401 ctagccaagg agaggcgatg tacatgaaca aagcaaataa caggcctaac ccgcccatca
     2461 tctgtaatgc acggataaat aatcgtcgtg aagtgccata ttcattcaat gaattatagc
     2521 gtgcagtttg ttttgcaatc gcagcaggta ttcctgctgt agagatcagt agaaacaatg
     2581 catatatatt gtatcccata ttgaataatc cattggcagc cttagcattt tcgcccatcc
     2641 aagcatacca gggaatgata tagacagcgc ctagcaatct tgaaatgata ttactggcgg
     2701 tcatccaagc agagcctcgt gccatttttt cttgcggtgt taattgatct aatgctggtt
     2761 ttctttgatt atccatcggt cagattccaa ctttctacaa aacattacta tcattttaat
     2821 tggtaaaaca gaaactggca atcgatttta tataactttt ataagaacaa ttgttttttt
     2881 gaattcggtt tcgttttttt ggtaaagtaa agaaatatac agccttttat ggctaacatt
     2941 tataggagat atcaatgaca caagaaataa aaaaactacc cgttatattt ttaggtcttt
     3001 tctttatcac agttggattg aattggttct tgctccctca cgatatagct tccgccggag
     3061 tgggcgccat cggacattta gttgaaacgg ccttttctat ccaacgctca gttactgttt
     3121 ggagcatcaa tcttatgatg ctgcttctag ccgctgtcct attaggaaaa tcagtatttt
     3181 ttaagactgt agcgggaagt ctgctttttc ctgttgtttt agagattgtt ccaaaagtgg
     3241 aaatgatctc ctcacatttc ttttcactga tttcaggaag tcttttgttc agtcttgggg
     3301 tttacacatt gtacacagtc ggggcctcaa atggcggtat aacgataccg ccgattattt
     3361 tccacaaatt ttttcggctt ccaatgccgc aaggactttt acttaccaat agtcttatcg
     3421 tctttttgaa ttattatgtt tttggattgt tggagacact attcgttctc ctatctatta
     3481 cagtcagttc attgtttatg aaaatactca tccgcttgtc ccctgtgtca agaaaatttg
     3541 aataaagctg gattttgtca tagcggaaac aaacgatata ttaaaaagag tgtgctataa
     3601 taaaccaaga aattgaataa agcgagggat caatcaatga gcttctcttt agaagacatc
     3661 cgtcatttac tattaaaaga aaatctatta aaagaatttg tttcaacaag aggctggcat
     3721 cttgatgtac cagaaaaagc tgaatttctt cagttaagtt atgattcaag aaaagctgat
     3781 gcttctactt tgtttttctg taaaggaatg aatttcaaag aagaatattt ggattcagca
     3841 atcgaacagg gtattcaata ttatgtatca gaacaaccat acgaaaattc agcagtagga
     3901 atcatcgtaa cggatatccg taaagcaatg gcgcttcttg ctatggcttt ttacgactat
     3961 ccgcaaaata aattgaaagt gatcggattc actggaacaa aaggtaaaac aactgctgcg
     4021 tactttacaa aagcaatctt ggatcacaca acaaataaaa aaacagcatt gctttctact
     4081 atgaatacaa cgttagatgg caaaacatat ttcaaatccc atttgacaac gcctgagtca
     4141 ttagatttat atcgtatgat gtcagaagca gttgaaaatg gtatgactca tctagtgatg
     4201 gaagtttctt cacaagctta caaaacacaa cgcgtatacg gcttgacttt agatgtcgga
     4261 atcttcttga atatctcgcc tgaccatatc agtccgatcg aacatccgac atttgatgac
     4321 tatttctatt gcaagcgaca attgatcttg aattcaaaaa cagttgtttt aaatcatgaa
     4381 agtgactatt ttgatttact tagggaaaca gccgatctat tccagattcc aacgatcact
     4441 tatggtcgtt cggaagaaag caattatcaa gtgatccgtt cagataaagg tactcacggt
     4501 tttactttat cttctcatga agatcgctta gctatctgcg atactgctta tgatattttg
     4561 ctggctggag gattcaacca agaaaatgca gccagtgcga tcattgccgc agcactaaca
     4621 ggtgcctcca gtcaagatgc acaagacgga ttaaaagaag ctcgtgtacc aggccgaatg
     4681 gatcaattga tccaatccaa tggagcgcat gtctatgtgg actatgcaca taattatcta
     4741 agcttgaaaa cattgctgaa atttgccaaa aatgaacatc cagatggccg tgtaattgtt
     4801 gtattaggca gcccaggaaa caaagcaatc tcccgtcgac atgattttgg aaaagtgcta
     4861 tcagaaacag ctgacatcgc ctttttgacg gcagatgacc cagcatttga agatccgaaa
     4921 aaaatcgcgg aagaaatcaa tgaagcaatt actaatccag atttgatcgt tcattatgaa
     4981 atggatcgtc ctgaagcgat tcgacgcgct ttagcagaaa gcgctccgca agattcagtc
     5041 gttattgcgg gaaaaggcgt ggatccctat caaaaaatca atggtgtaga tgaaccttat
     5101 gaaggggatt atgcaatcgt gaagcggttg attgaagaat aatggtattt atatactagt
     5161 ggtttcccat ttctaagaaa aaaagggact gtgatttttt gtcacagttc cttttttctt
     5221 agaaatcatt ttaacttttc actagctaat tctgctgcct tttctctttc ttcatttaat
     5281 aatttattat catagaattt gataaatggg aaccagataa tgaatgcgat gattgcacaa
     5341 atgactgcta acacagcagc tttccaatca ccaccactac caacaaatgc tccgatcccg
     5401 actggtgaag gccacggcat ctgtgcaagc atcggtttga cgatttccag ccgaatggca
     5461 aaataagcga gtgtagcaga agccatcgga gctaagaaaa acggaaccgc taaataggga
     5521 ttgtagacga ttggcatccc gaaaatgatt ggttcattga tattgaataa tcctggtaca
     5581 agtgaggctt ttcctaacac tttcagctga tcagatttcg cgaggaatgc aataaagatg
     5641 atcaatccta aagtcgcacc tgatccacca acggttacat aagaattgtt gaattctcct
     5701 gccaacggat aatttgcgcc ttgtgcgtta gctgccatat tagctaatac gatcggggtc
     5761 aagaaagaag tgatgatatt cgctccatgg atccctacga tccacaatgc atggatcaag
     5821 aaataaatca ccatgacacc aagccatgta ttcgtcaact gcgtaacaaa cccaaatggg
     5881 atagcaatca ttttgaagat atccgttcct agtgcaacta ataatccatt gatgatcatc
     5941 accacaaacg caatgacaaa tgttgggatc agagcagtga aagaacgaga aactcctggc
     6001 ggaacaacat ctggcatttt tatgacccaa tttctcttga cacacataca atacaattcg
     6061 accgcaataa tcgacataat aatagctgta aagattcctg aagttcctaa acgttcgaca
     6121 aaagagccca tcctaatacc atttactaca gtttctgtct cattgataga agcaatcaag
     6181 ctgattttac catcagtcat gatcaattca gggatacaca taaagaatgc aaaaacagat
     6241 agtaatgccc ccgtgagcgg attaacattt aatttttgtt catctcgttc aattgacgtt
     6301 aattcataag caaacacaat ggcaaagtaa atagataaaa tccccatcgt cattgtattg
     6361 acgatcatgt acaagtcact aactttgaaa aaagttgcat taaaaaatcc ttctaaaaat
     6421 gggaacggta atggcaaaat atttaaaacc aaaaacatcg agcctacaat cgtgaaagga
     6481 atagaagcca tccccgctgc catgacagcc cgaacgattt taagttgcgc aattttcccc
     6541 ataggtccca tcaggtattt ctgcaaaaaa tcaaacatta gttattctcc tttcctaaat
     6601 ataacgcttt ttcatattgt ttaatacgga tatactgttg gtcttggttg agtgaaattt
     6661 tctttaaacg atactgtata aatgcactgg caaaaatacc gcttacaaca agaatgaaaa
     6721 caaataactg tcctccattg ttatttgcca taaaaggata caagaatgta aatcctgagg
     6781 taaacaacac tggaatcact aataaattaa agacactctg gaacaaaaaa taccactttg
     6841 tccatggaac gttcgtttgg tgttttcgat aaagctttac ttgttctcct actgcaggca
     6901 aaatagctaa aatcagaatc atcggtaaac tacttcctaa acttttagct gatagaagca
     6961 gaatcatcca ttgcatgttc atgaaaaaga aaaaggcagt caggtaacgt acgattagat
     7021 aacgattgaa atacatgttt ttcaaactaa gttgcttttt ttctaattcg attttccttt
     7081 ttactgtatc ctgcacaatg atccttcctt tctatttatt tttttgatac aaataattca
     7141 tttcctgtgc aacttccaat aatgtcatcg tcgtcatcag atgatcttgt gcatggacca
     7201 tgataatttc catttctatt tttgtcccgc tggcatattc tttcagaagt tgagtttgtg
     7261 actgatgcgc atgaagaatc tcttcatttg cttgtcgcaa cttctcttct acttcttcaa
     7321 acatctcttt acgcatcgag cgaaatgctt catgtatcag tgtccgtgca tttccgctat
     7381 ggaaaatgat atcaaaagaa gtcacttgta gtgtttcact gtttattttt tcagccatct
     7441 gttttcctcc ttattcgatc agtttgataa atttactgct gaaattctca aaaataggtt
     7501 cagctaagat ttcctgctgt acttttagct ggtcaactaa cctaacgatt gctttagtca
     7561 cgaccgtaat tccttcattt tcgacataag aaggcgaaac taaaaaaaca aaatgaattt
     7621 cttgatattg atcccactgc atcccagaag gaatcaatgc aacggctacc tttgtttcga
     7681 ttccaactgg aacagcggga tgtgggactg ccactgtatc actgaatatg atttgtccca
     7741 tattttcacg ttgctggatc tgctttcgca tttctcttat atatccttct tcttcattta
     7801 tgctcagctt ttgcagcaaa tcatgaagaa tttcttcctt tttcggttcg tacgtataag
     7861 tcttgaaaaa tttttcagac aactgttcgg tgtaaatctg ctttttttgc tgtagagaaa
     7921 atgctggtat cggtctatct gaggaatgat agggtataga ttctccaact acctttcgta
     7981 ttttacgaac atcatcttca ttcaaaaaaa tactgacatg taaaactgga attttgaaaa
     8041 acattgtcga aagatcaata gaagaaataa tcaggtcgat tccttttaaa gttgtttcat
     8101 tgatctcata atagcctttt acacttacca ctgtaatatt tttaccgaat tcactgacta
     8161 cgcgattctt taatagttgg gcactgccgt aacccgttgc acagataatc aatgcatgta
     8221 gtttatgcgc agctctttct ttttccaatg ccgccatgag atgcaaagcc aaataggccc
     8281 attcatcttc attgatggta tagtttttca aagccggcat gttgctgaaa tgccttttta
     8341 ctgcgtcaaa gactttggga ttttctttct ttatctcctc agtcaatggg ttctcaatgc
     8401 gaatacctcg gctcaatcga atcatcatcg gtgacaaatg atcgagtagt ccattgaaaa
     8461 gctggagatc ctctactaat gaaaaatgaa attcctgaga caatcttttc agaactttcg
     8521 ctaattcctt tgatagttcc tgatcatttt gattttcttt gtgattcgac ttagccatga
     8581 gatgtaatgt caaataagca atttcttctt cgggaaacga tacacctgcg attacctcaa
     8641 tacgttgaac gatccgctgc gccacttgat actctactcg cttagaaact tcttgttcga
     8701 tgcctaattc cttgatttca aacccttctc ggagccgttt gatccccaaa gccaagtgta
     8761 aagtcagatt ctgtatgata aaatccgaaa cttttaactt agcttctctt gtttcatcga
     8821 gaatgataat gactagctct tcaaaactga tgtctgggaa aaaacggctt tgccctaaat
     8881 attctttcaa tgaatttcca taatggtttc caaaaaaagt atccatgata aaatgccgtc
     8941 gatttctttc ttccccttcg atccatgtcc cttgaccatg cttagaaacg atttttagtg
     9001 aatatggaat caatcgctcc ttgatttctt gcatgtcctt tgtcaaactg gaacgactga
     9061 tataaagctc ttctgccaaa tcatccataa aaagaatggc atcttccaaa agcaattggt
     9121 tcaatatata cttttgtcga tcttcggact cataaaatcc ttgcggttct ctagcatatc
     9181 caggtactat ttcttgccga ctcaaaaaca agtcaaatgt cagtctttgt aaaatatgca
     9241 gttggtagcc ataacctggt ttagcaatga tcttacctcc gtttttttct actaactctt
     9301 tgatccgctg caagtaatta cggattgtcc gatcagataa taacaattca gaagctaaac
     9361 gttgactggt gacatactcc ttcttgtgat caatcaaata tctcaacagc aatttttcct
     9421 tctgtttcat tatcatccac tcctattcca tcatatgttc tcttcgatca cctctgctaa
     9481 cttctggatg cctgtaggga ttgggacata cgcttggaaa gggatactaa caacaggctt
     9541 tccaacagat tctcctaatt ttttgaattt atccaaaaac atcgtcgttt gcgggctgat
     9601 cagaaataaa tcaaaatcac tattcttaat catattatcg ccttctgtag ctgaaactgc
     9661 atctacgagt acctcttctc ttttttgctt aaagaaatcc gttgtctttg aggccatcat
     9721 cgaggatgac attcctgccg cacaaataat caatgctttt ttcataaatc aatcactcct
     9781 tttattttta taaaccaagc atatcttgta agcgttttct attcaaaata tatatttccg
     9841 tagcattgcg gaaaaaatgt gtatcatatc ttgaataata atttctggca ccgtgatata
     9901 ctagtgatag aattttttca ggaggggatt tagtgagtat cacagtgaat attttgtata
     9961 caagtacaaa tggacaagcc agaaaatttg cagaagaaat ggtcgaaaga aacattgtca
    10021 aagcgattcg tgcagaagaa ggaaacgaga aatatgacta ttactttcca ctagaagatc
    10081 cagaatctct tttactgatc gaccgttgga aagatgaaga agcaatcgaa aagcatcaca
    10141 aatccgaaat gatggctcaa ataaacgaac tccgtaaaaa gtataaatta aaaatgaaag
    10201 tcgaacgttt cactgattta ccgtaaaaag atacacatgg aatgatagca atttatcatc
    10261 catgtgtatc ttcacttttt ccttattttt tattcgatct catctaatga ttcaaaccgt
    10321 gtttttctct tatttttcgg ataagtattt cagtcacccc aatatacatg attcctaaac
    10381 ataccccaag gatattctca aaaatgcgga tcgttactgc tcctggaacc ccgaataaac
    10441 tggcagtgat tgccaaagca ccaaaagaat taaaaactgt tttatatcta taggtagaac
    10501 aaacacctaa agctagacca cctaatatac cgacccagtt aaaaggaata aattggctga
    10561 taccaataaa taaaacacat ccaatcaatg agccaatgat tcggtctact gctcgttctt
    10621 tgaaaccaat cgacatcaat ccatagctag atacaataga agaaaatgca aaccctgccc
    10681 acatcaatcg ctgaaaaggg acatactctc ctgcaacgat caaaagacta atgccaaatg
    10741 catagatgag catccaaatt ttttcctgcg aatacatgcc tttcaaaaaa atctctttaa
    10801 acaagctttt gtctttgttc ttttctcgat gttttctgta aaggagaact gaaaaccaac
    10861 aaaaaaacaa aaataataag ctacttgttt gtgtaaagaa attcttgttc aacgaatctt
    10921 taggtaacga ataaacaata aagagatatg aaaagccgta caaagaagca ttccccattt
    10981 tgggatcact agcggttgcc attaatatgc tgcttagtaa aataaaatga atcaacatgc
    11041 ccataactga ccatttgatc agctggacat acggtgctac taatagtgag aacatgacga
    11101 ttgctaaacc tatgatcgaa tgggaaatct tataaccaaa gtcaacaaaa cgaatactca
    11161 tcaacataca gaataaaacc acagcgtatg gtttatgctt cgattcaaat aatgttgtaa
    11221 ttataatgat aaaacaaatg gcaaacaata cgatgaataa tgatcgtaaa aacaatgaac
    11281 aaatatacat gcacttttct tttttagaag ctgcttctcg tattttttgt tttaaaataa
    11341 acggatctag ttgtagagac tgataaaaat ccacctgttg tcgtcctttc tttttacttt
    11401 tattttttac agtcatctat gacaggttgt aaatactatg gaacatcata tggcgttgtc
    11461 attttcttgt caactatttt tcctattcat gtttttataa aaaaacatat cgttttcttg
    11521 tatgattaaa tcaccacaaa ataaacaact gagagaacga tatgctttat gaatggttct
    11581 atcaaaaaaa ttctgagatc aatagacaaa aatttgatga ttacagaggt ctcttacgaa
    11641 accttacaga agaaaaaaac attgatcgtt gctgcagttc tcttccctgc tccatgtgtc
    11701 tgtagaaatt gtggttctgc tgtggtagat ggaaatagga aattaatggt ggtgaaaaac
    11761 aagaagaagg aaaccattgt ccactttgaa taatataatc atatgccaat ggttatacgc
    11821 ctaaaaaagc agcgctatac ctgtaaaaac tgtcgaagcc attggaacgc tcaaagttat
    11881 tttatccgtc caaggcactc gatttcaaat catgtcagac ataaaatcac ttctttactg
    11941 actgaaaaag tgtcgttatt ttttatttcg aaaagttgtt aggtatcttt gacaaccgtt
    12001 attcgcacat tgaaagagtt gagaagctat ttaccaaagt cttccaaaag aattctgtct
    12061 tgagtgttga gggttgtgaa tttcgttctc acgtttccgt tgaggataaa ctgagtttta
    12121 tttccgcgga tggcgagaca gaaaaattaa tcgagaaaat ttacctacac aaaaaattcc
    12181 tcgattaaca aactatttcc ttagctgcac taatcctaaa gaaatagaat tcttagttac
    12241 agacatgaac gccacctact ttcagctcac caaacatgtt ctaccaaatg cgaaattggt
    12301 gattgatcga ttacttggtt tttccacgcc tttcaaggag gcgtaacctt tattcacgag
    12361 ttggttgaag ctttccgaga caaaagaccc gacttgtttt tctccttatt gacaaagagc
    12421 cattaaaact agagaaaaaa ttacaactga tcgctgatta tttgtagaaa gaaaatcaaa
    12481 gtattaccca ataaacttga gtcattaggc aaaaaacact gcgaaactca agaatgattt
    12541 tcgcagtgtt tttgtatatt ttttattaac tagtattact acaaaggttt atttttcact
    12601 tctcaacgca gcacaaaatt gtctttactt atttactggt tgggttcaac cttctctact
    12661 ccgatactaa tatttgtttg tatacccctt ctaaaatcgg tagtttccat tcaattgatg
    12721 ggtactagtt gattttaaaa actatgctta ttcaattttt tattttagct catatatttc
    12781 gtttctttct aaattttata ctgatgaaaa caagtagcac acccaatatt gacaatataa
    12841 actggctaat gatggtttct ccagtttttg gaagatgctt atttccactg cttgttggat
    12901 tgtttcctct tatatctgag ctatcagcaa cttttctatt ctcttttgtt aaaaagatcg
    12961 ccacttcttg ttctttttcg atcttgaaag ttactggtgt cgcatccaat tcataccccg
    13021 ttggcgcttg agtttctacc agctgatatg ctcctggtgc taagccatct atcgccagtt
    13081 ttccatctgc tcctgtggtt agacctgact gcagaacggt tccattggca tcttgtaact
    13141 caaaaacagc tccttgcagg acttctccgc tttggtcatc gatctttgtc aacacgacac
    13201 cgcctggtgt aagtcgattt tctttcgtca attctaccac cgctgtctga cttctttcga
    13261 tctcaaactc gattggtgtc gcatccaatt cataccccgt tggcgcttgg gtttctacca
    13321 gctgatacac tcctggtgct aagccgtcaa tcgccagttt tccatctgct cctgtggtta
    13381 gacctgactg cagaacggtt ccattggcat cttgtaactc aaaaacagct ccttgcagga
    13441 cttccccgct ttggtcatcg atctttgtca acacgacacc gcctggtgta agtcggtttg
    13501 ctttcgtcaa ttctaccacc gctgtctgac ttctttcgat ctcaaactcg atcggtgtcg
    13561 catctaattc atacccc
//
LOCUS       DACEXQ010000052        13470 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.052, whole genome shotgun sequence.
ACCESSION   DACEXQ010000052 DACEXQ010000000
VERSION     DACEXQ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.052"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(334..2460)
                     /gene="pnp"
                     /locus_tag="IXH04_001115"
     CDS             complement(334..2460)
                     /gene="pnp"
                     /locus_tag="IXH04_001115"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAQ1851735.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(2648..2917)
                     /gene="rpsO"
                     /locus_tag="IXH04_001116"
     CDS             complement(2648..2917)
                     /gene="rpsO"
                     /locus_tag="IXH04_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAQ1851736.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            3168..3731
                     /locus_tag="IXH04_001117"
     CDS             3168..3731
                     /locus_tag="IXH04_001117"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ1851737.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(3984..4505)
                     /locus_tag="IXH04_001118"
     CDS             complement(3984..4505)
                     /locus_tag="IXH04_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAQ1851738.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(4508..5350)
                     /locus_tag="IXH04_001119"
     CDS             complement(4508..5350)
                     /locus_tag="IXH04_001119"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAQ1851739.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(5343..6068)
                     /locus_tag="IXH04_001120"
     CDS             complement(5343..6068)
                     /locus_tag="IXH04_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAQ1851740.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            6244..6540
                     /locus_tag="IXH04_001121"
     CDS             6244..6540
                     /locus_tag="IXH04_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851741.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            7108..8700
                     /locus_tag="IXH04_001122"
     CDS             7108..8700
                     /locus_tag="IXH04_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1851742.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            8713..9453
                     /locus_tag="IXH04_001123"
     CDS             8713..9453
                     /locus_tag="IXH04_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ1851743.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            9621..10970
                     /locus_tag="IXH04_001124"
     CDS             9621..10970
                     /locus_tag="IXH04_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1851744.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(11098..11688)
                     /locus_tag="IXH04_001125"
     CDS             complement(11098..11688)
                     /locus_tag="IXH04_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAQ1851745.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            11835..13187
                     /gene="brnQ"
                     /locus_tag="IXH04_001126"
     CDS             11835..13187
                     /gene="brnQ"
                     /locus_tag="IXH04_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ1851746.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         3789 a         2655 c         2655 g         4371 t
ORIGIN      
        1 tttgacctga gtattagaga aacaaacgga aaaatagttc ctcatatttt gccgtttttt
       61 gagctaatat cgagggacag ctgattgaac atcgtttatt ggagaggttg taaaaaagct
      121 gttctgcatc aagtaataag aacagccaag taaaaacagc tcctaatgtt tttaacttgg
      181 ctggacttat taccgcagat gcaagctttt gaacaccgtt tattcgaaaa aaaaggactg
      241 tgacacggct tttgtcacag tccccttttt tcgaataatt attgtccccc tgtcaccttc
      301 acgatcacaa gtacattatt cctatgaatt aaattatttt tcttcatttt tcgttgaacc
      361 atcttcgttc aacattgctt ttcttgacag atttacgcgt ccttgtttat cgatttcagt
      421 tactttcaca aggatctcat cacctagttt aacaacatct tcgactttat tgacacgatc
      481 gtttgctaat tgggaaatat ggacaagtcc atcttttcct ttgatcaagt tgacgaatgc
      541 accgaatttt tcgatacgga caactttccc tagataaact tcaccaactt tgacttcttt
      601 ggtcaaatct tcgatgatct tgatagcttt tttgatcatt tctgcatcag ctgaagcaat
      661 acttactttt ccatcttgat cgatatcgat tttcacacca gtttcatcga tgattccgtt
      721 gatcgtatcg ccgcctttac cgataacatc tttgatttta gcaggttcga tctggatcat
      781 ttcgattttt ggtgcatatt ggcttaattc ttcacgtgga gcagcaatcg ttgaagtcaa
      841 ttcttctaag atttccatac gtgcttgttt tgcttgagtt aatgcttcag ttaggatttg
      901 ttctgtgatt ccttgaatct tgatatccat ctgtaaagca gtgataccat cttttgtacc
      961 ggcaacttta aagtccatat cgccaagatg atcttccagc ccttggatat cagttaggat
     1021 cgtatagttt tcaccatcag aaacaagtcc catcgcaata ccggcgactg gcgctttgat
     1081 tggtacacct gcatccatca atgctaatgt gccagcacag atacttgctt gggaagaaga
     1141 accatttgat tccaatactt ctgctactaa acggatcgta taaggaaatt cttcttctga
     1201 agggattact tgtgctaacg cacgttcacc taatgcgcca tgtccgattt cccgacgccc
     1261 tggagagcct gcacgtcctg tagaaccaac agaaaattga gggaaattgt agtggtggat
     1321 gaatcgtttg ctgacttcca cccctaaacc atcaatgatt tggtgctctc ctagaggtgc
     1381 gagtgtacaa gctgataaag cttgtgtttg tcctcgtgtg aacaaacctg atccatgtac
     1441 acgtggcaat aagctgacct ctgatgacaa tggacggatt tcatccaatt tacgcccgtc
     1501 aggacggatc ttatcgatgg tgatcaattc acgtacgaca tctttttcaa gatcttctgt
     1561 gatttgttta acttctttca acaagtgatc ttcatcttca tgacctgcaa atttctcagc
     1621 ataagcttcg cggatatcga tcttcacctg ctcgatatta tcttcgcgag caagtttttc
     1681 ttcagtcatt actgctgctt tcattgtagc gtaagaagca tcaaagattt cttttttcaa
     1741 tacttcatca acttgcagta aagtcacttc cattttttct ttccctactg cttgaacgat
     1801 ttcttcttgg aaagcaacta actctttgat agcgtcaaaa ccaaacagca atgcaccaag
     1861 catgtcttct tctgaaactt ctttcgcgcc gctttcaacc atattgatgg cttgtttcgt
     1921 tccggcaaca gtcaattcga tatctgtttg ttctgcttgt tctactgttg gattcaagac
     1981 gtactcgcca tttacacgac caacttctac gcctgcgatt gggccgtcaa atgggatatc
     2041 tgagattgat agtgctaaag aagaacctag cattgctgcc atagctggcg agcagtctgt
     2101 ttctacactc atcacgatgt tcgttacttg gacttcatta cggaagcctt ctgcaaacat
     2161 cgggcgaatt ggacggtcga tcaaacgggc agtcaatgtt gcttccgtac ttggacggcc
     2221 ttctcgtttg atgaaacctc cagggatttt ccctgctgca tacatttttt cttcataatt
     2281 gatcgttaat gggaaaaagt ctgtatcttt tgcttcttta gaggccacag ctgcacttaa
     2341 gactactgta tctccataac ggactaaaac ggctccgttt gcttgttttg ctagctgacc
     2401 gacttcgatt tgtaagggac ggccgcccca tgtcgttttg aatacttgtt tttctgacat
     2461 aacttctcct tttccttctc gtgaaagcaa gacgctacta aacaaatgaa aactgccgaa
     2521 agccgtcttt tgagcagctt tcatttggtt agtaggagtc catctagctt gatcacggaa
     2581 ttttacttaa ttgatatacg agtaaaaaaa gtgagattca tgtgaatccc actctttttg
     2641 actaaaatta acgacgcaag cctaaacgtt tgatcaattc gcggtaacgt tgaacgtcag
     2701 ttttacgtaa gtaagctaac aagttacggc ggtgaccaac ttttttcatt aggccgcggt
     2761 atgaatgatg gtcttttttg tgcacgcgtg catgttcatt caagtggttg atttcttcag
     2821 ttaatacagc gatttgtact tctggagaac cagtatcccc ttcatggcga gcgtattcgt
     2881 tgatgatttc attcttgcgt tcttttgaaa ttgccatttc tttcacctct ttcgattatt
     2941 cccctgcgac tgagtaaaac gttggtgatt cgcttaacca agtggcaggt catgtgccta
     3001 gagcacacag ctatacttta cctgaaaccg ggttgaaaat caagtagtta atcattaaga
     3061 aaaggtagtt ttcaaacaat cttactacta cttttccatt tttgtggtaa actataatgc
     3121 gaaaatagac ttgaaacaca gtcttttaag aaaggggtac atattccatg attacaatgg
     3181 atgatatcat tcgtgaagga aatccgacct tacgcgaagt tgcaaaagaa gtttctcttc
     3241 cattaagcga agaagatatc tctttaggaa aagagatgct tgagttctta aaaaacagcc
     3301 aagatccaat caaagctgaa gaactacact tacgaggcgg tgttggctta gctgctcctc
     3361 aattagatat ttctaaaaga atcattgctg ttcacgtacc aagtcctgac ccagaggcag
     3421 atggaccttc tctaagtact gttatgtata atcctaaaat tttgagccat tctgttcaag
     3481 atgcttgttt aggggaaggt gaaggctgtt tatctgttga tcgagaagta ccaggatatg
     3541 ttgttcgcca tgccaaaatc actgtgtctt attatgatat gaatggtgaa aaacacaaga
     3601 ttcgtttgaa gaattatgaa tcgatcgtcg ttcaacatga aatcgaccat atcaacggtg
     3661 tgatgttcta tgaccacatc aacgatcaaa atccatttgc actaaaagaa ggcgtattgg
     3721 tgatcgagta gagaattggc aggttccttc gatactatgc tacaagaaag ctttataaaa
     3781 aaggggagtg ctcaaaagct gcacttccct tttttatgtt tctccactaa tcgaaattta
     3841 gatagacatc tagaaatcgc aattccaaaa aattctgtgt attggatacg catcttttct
     3901 tattttttct tatttcttcc atatgggtgt ttatctcaga gatgatagaa tagtttctag
     3961 gataaaaagt agcagctacg atgttatcct tcgtagagga ttcctgcttc agctagtttt
     4021 tgtttgatgt ttttcagcat atgcttttct gggtagcgga tcgtatgcag atggatgccg
     4081 cctgttaatt cggataacaa agtagcatta cttttttgat aacgtctcat aaaggaatcg
     4141 atatcttgtg gcgttttgat gtgcaaagtt cctgctaact caccgtaaag gggatgttcc
     4201 acaattacat caatgatttc accaccattt tcaacgatta gtctcagttc ttcttctgtt
     4261 tgatcttttc catgctggac agcgatttta ctgatgtgcc cagtattggt ttcttctgtt
     4321 tcgaggatat aacctcttgc tgttgccacg atacttttac ctgcagctcg tagtaaagca
     4381 atatcgccta caatgatctg acggctgact ccaaaagttt tcgcaaactt gctagcactc
     4441 actggttttt ctgtatttgc caaggtttgc atgatttgtt ttcgccgtgt ctctccttcg
     4501 atcacactta ttcctccttc attttgtttt ggtattcgcg cgagtgtttc atcaaatcaa
     4561 catacgtgtc tgttattgtt tccttatatc ccttattttt cagcagacta acaactttat
     4621 ccaatacttg ctgctctctt tttgcatcat aaactgcttg gttttcttct tttttcactt
     4681 gtcctatttt agttaccgta tccattcttt tttctaataa agcaaccaac tctcgatcga
     4741 tcatatcgat cttatgacgt aatgtttcaa gagactcttc tgttagttct tcctctactg
     4801 tcttgatgat acgagcagga tttcctgcaa tcactacatt gtcgggaaaa gatttggtca
     4861 caaccgaacc tgcgccgact actacgttgt cccctagagt gacacccggc gtgacgataa
     4921 ctcctccgcc taaccaaaca ttattaccga ttttaatggg ttttgcgtac tccagaccgc
     4981 tgttacgttt cactggatgt agtggatgcg tcgccgtgta aagctggaca ttcggagcga
     5041 acatgcagtt atcaccgatt tcaatcgtgc tgacatctaa aaatgtacaa ttgaaatttg
     5101 cataaaaatt ctccccaaca aatatattat atccgtaatc aaaattgata actggctcca
     5161 tatagatttt tttccctgta cggccaaaag tctctttcaa caattgggat cgcagttccg
     5221 ctgattctgc ttggttgatg atttggcttt gacttcttgc aaacatacgg tcggcaacca
     5281 gctcaggatc attggcaaaa taaagctcgc ctgcaatcat tttttctttt tcggttttga
     5341 attcagtcat aatctgctcc taattcttgt atttttttga aaacaaccgc tccgtcttct
     5401 tgaataaacg tataaggctc cgctaaataa acaggcgttt gattgattac taagattttg
     5461 gctgacggct ggcgaaactg taccagatca caaaacggat gaacttgaaa actcgttcca
     5521 acaatgacga tcagctctgc ttggctgacg gcataagccg cctgctccat gactccatct
     5581 tgaaaccctt caccatataa cacgatgtct gggcgtattt gtcctccgca tgcttgatgt
     5641 ttgtcgctgt gaagatattc tttccagtct accgtttgtt gacattttcg acaatgacat
     5701 tgatacagac tcccatgaaa attgacgagt tgcgggcttt tagcctttgc gtgcaaaccg
     5761 tcgatgtttt gggaaacaac ccaaacattt ttttctttcg ctagatgtgc tatttccaaa
     5821 tgaatgatat tgggttttgc ttcaggatga taaatctttt tgacaaattg atagaattta
     5881 tccggttcat tcaccattgc ttgatgactc agcaagtatt ccggctgctc catcccgtgg
     5941 tagattcctg acaaagatcg atagtctggc actccagaag gtgtggatac ccctgcaccg
     6001 gtcaaaaatg taatttgctg ataagctaat atctcttttg ctgcttcgtc tactgttaat
     6061 tgtttcatgt tttgtcacct cactttccat tatagcccat actcgaacaa atttattgtt
     6121 gaaaagtcag aaaaatggga ataaaaaagt gttatgggca tttgagcaat ttatcacaat
     6181 gttcactgac aaaagaatga aaacgtgtta caatactgct gtataaaagt gaggtgaatc
     6241 gatatgttac cattctttat ttattggggc gttattctgg cttgtatcgc ctggttagcg
     6301 ttaagcgttt atttctctgt tttttatctt gtacgcaaag aaaatggaaa tctttgggct
     6361 tttgctttat tcaacgtgat tgccgcaatt gttttagcca ttacattagc cgtatatcga
     6421 acatggggct ggggaatcac ccaatactca agtttgattt atctaatatt agccatttat
     6481 ggcgtcgttg ttcttttgca agcgatccta ggacgtgagc caaagaaagc ggcagcttaa
     6541 atgatcgaga tggaactgga cacaggaaat tttctgttct agttcttttt ttattgtatt
     6601 acatacattt ttgtgtataa ttatactatc gtttgcaatg gatagatgat tcacattttg
     6661 attgtttttt cggtaaaagt aaagatttct atatttatca gtattttttc aaaaaccagc
     6721 agttatagat tgacaaagcc tctcgagtat cgtaaacttt ttttgtataa ataactgaat
     6781 acgagatgcc tcaatcaatg aggtagaggt cgcgagaatc attaaccctg tggagtgaag
     6841 caaacatgga gaagcaggaa tagggaggat cgccgaaatg tgcacttttt tgcttggagt
     6901 gtatgttggg acgcaagtta agagcttgcg gactgtctta gcagtgatgc taagttgcgc
     6961 tatgttttgt gagaggttta tcaccttatg gaattttagc ggccttttta tatctataaa
     7021 tcggtcgctt tttatttttt cagataaagc tttaccgcat ttcatctaga ccgctcgtgt
     7081 atcatataat cagaaaggga gaaaaaaatg aaaaaaaata tggtttcagt tgccgtacta
     7141 tttgcttttg tcttctcact attcagtgtg ggatctctgg caaatgcaga tgaaaaaaca
     7201 ccgagcgatg agtttcgcgt aggtatggag gctgggtatg cacccttcaa ctggtctcaa
     7261 caaacagatg agaataatgc cttgccaatt caaggccaaa atagttacgc cggcggttat
     7321 gatgttcaaa tcgctaaaaa agtagcagat ggtttaggga aaaaattagt catcgtccaa
     7381 acgaaatggg atggtctggc tcctgccctt caatcaggca agatcgacgc gatcatcgcc
     7441 ggaatgagcc ctactgctga acgacgaaaa gaaattgatt ttaccgatcc ttattacgaa
     7501 tcacaattag tagtagtagt tcaaaaagat agcaaatatg caaaagcaaa aagtttagca
     7561 gatctttctg gagcaaagat cactgctcag ttaaatacat tccactatac tgtgatcgat
     7621 cagataccag atgtgcagaa acaacaagca atggataatt tctcagcaat gagaacagct
     7681 ttagcttctg gaatgatcga tggttatgtc agcgaacgtc cggaaggtgt aacggcaact
     7741 agtgtgaaca aagacttgga aatgcttgaa ttcagcggtg cagatggctt ccagacaaac
     7801 ccggaagatg ttcagatttc tgttggtatg cgtaaaggcg acccggatat ccaaaaagta
     7861 aaccagattc tttctggcat ctctaacgat gaacgaacca aaattatgga ccaagcagtc
     7921 aaagatcagc ctgctgcaac tgatagtgaa gaaaaacctg gcatcatttc tgatttcact
     7981 acgatttgga aacaatacgg caatatgttc ttacgaggtg ctggacttac cttgttcatc
     8041 gcattgatcg ggacagttgt cggtactaca cttggattac tgattggtgt cttccgttcg
     8101 gtacctgaat caggaaatgc actgactcgc ttcttccaaa aaatcggaaa tgcacttctt
     8161 tctatctatg tcgaagtatt ccgcggaaca ccgatgatgg tacaagcaat ggtcatcttc
     8221 tacggtctgg cactcgcttt tggcatttca ttgaaccgaa cgattgccgc tttgtttatt
     8281 gtctctctca atactggagc ttacatgaca gagatcgttc gtggggggat ttttgcagta
     8341 gatgaagggc agttcgaagc tgcgcaagcc attggtatga cccatggcca aacgatgaga
     8401 aaagttgtta ttccacaagt tttacggaat atcttgccag caacaggaaa tgagtttgtt
     8461 atcaatatca aagatacagc agttttaagt gtaatcggcg tagctgattt gttcttccaa
     8521 ggaaactctg cttccggtgc gaatttccaa ttcttccaaa catttacgat cgtcgggatc
     8581 atctacttag tcatgacctt tacgatcacc agaatcctgc gggcggtaga aaagaaaatc
     8641 gatggtcctt cagcttatac caagcttgaa gaaatcgaca attcaaattt gcaggaataa
     8701 ggaggaagaa tcatgaatga aaaaattatt gaaatcaaac atctaagaaa aagcttcggt
     8761 gaaaatgaag tattgaaaga tatcagcgta acagtcaata aaggtgaagt tgtgacgatc
     8821 atcggttctt ccggttctgg taaatctact ttcttgcgct gtatcaattt actggaaaaa
     8881 ccaacaggcg gcgagatcat ttataatgga gaaaatgtat tagctcctaa atataattta
     8941 cctaaatatc gaacgaatct aggtatggtt ttccaatcct tcaacctgtt caacaatatg
     9001 aatgtcttgg aaaactgtat gtctggtcaa acaactgtcc tgaaacgaga taaagaaact
     9061 gccaaaaaag tagcaatcga aaatctggaa aaagttggta tggatcgtta tatcgaagcc
     9121 aaaccagcac aattatctgg tggacagaaa cagcgtgtcg caattgctcg ggcattatca
     9181 atgaatccag atgtattgct attcgacgaa cctacttcgg cgttagaccc tgaaatggtt
     9241 ggcgaagtgc ttaatacgat caaagactta gcacatactg gcttgacaat gcttatcgtg
     9301 acgcatgaga tggaatttgc aaaagatgta tctgaccgtg tcatttttat ggataaaggt
     9361 gtgattgcag aagaaggtac accagaagat atctttgtcc atccgaaaga agaacgtaca
     9421 aaagaattcc tttcacgtat tttaaatgct taggatcaat agctgattgg gacaaagagc
     9481 tgcggtgttt accgcagttc ttttttatga attactcata aaaggtaaat ttctgaaaat
     9541 tttggaatct tagttgtgtt tttcagaaaa ctctcttaat atggaagtaa ttaaactttt
     9601 ttagttagga tgatttaaaa atgaatgaag agcaagaact gcagcgtgga ctgaaaaata
     9661 gacatgtcca actgatttct attggcggag cgattggaac ggggttgttt ttaggttccg
     9721 gaaagtctat ccagctagct ggcccttcga tattgctcgc ctatcttatt actggatgca
     9781 tctgtttttt cattatgcgt gcgttaggcg aacttctgtt atcaaacacc aataaccatt
     9841 cttttctgga ttttgttgca gaatatcttg gcaaaaaagc ggctttcatc actggctgga
     9901 cgtattggtt ctgctgggta tcgattgcaa tggccgattt aacagccatc ggaatgtatg
     9961 tacgctactg ggcgcctggt gttcctcagt ggcttcctga actgattgca ttaggtcttc
    10021 ttctttgctt gaatatggtg gctgtctcat tgtttggtga gttagagttt tggtttgcat
    10081 tgatcaaagt tgtagcgatc attgccttta tcctcgtagg agcttatatg atcctcaccc
    10141 actatacgac agatattggc acagccagta tcacgaatct atggtctcat ggcggattct
    10201 ttccaactgg tgcaaaagga tttatcttag ctttccaaat ggtaaccttt gcttttgctg
    10261 ggatagaatt agttggatta gtcgctggag aaacagaaaa tcctgaaaaa gtgttgccgg
    10321 aagctatcaa taatattcct atcaggatca tcttatttta cttaggatct ctttttgtca
    10381 ttatggcaat ctatccatgg aacagcttga atccagataa cagtccattt gtagaagtat
    10441 tttcggaaat tgggatcacg attgctgctt ccttgatcaa tttagtcgta ttaagtgcag
    10501 cagcttctgc ctgcaacagt gcaatatata gtacaggacg tatgcttcgt tctttagcgc
    10561 aagaaggcag tgcccctaaa aaattcaaaa agttaacaac acatcatgtg ccagggaatg
    10621 ctttgacatt ttctactatc gtcattttca tttctgttat cttgaattac gtgatgccta
    10681 gtgaagtatt cacactagta agcagtattg ccactacttg tttcttgttt atctggagta
    10741 tgcttgtcta cacgcacatg aaatatcgta aaagtatcct cggcaaaaaa gctcattcat
    10801 ttaaaatgcc gttgtaccca tttagtaatt atctggtatt tctttacatg gcctttgtgt
    10861 gtgtcgtctt gttcctagga aaagatacga gaatcgcttt attactaaca cctgtgtggt
    10921 tcttgcttct attactaatc tatcatatga aatatgaacg taagaagtaa gaaagtagtt
    10981 gtgacagaag tggtcagctt cgagaaataa gaaggaattt acgaaaattg cttcttaaat
    11041 ttttgtgaaa tttcggctta tttcttggag ataaaagctt ctgtcccagc ctcttttcta
    11101 tttccccgga aacaaccgct gataataggc atctgctttt ttataggaaa aaatccaaat
    11161 accagataca aaaaaacgtc cgaataaccc gatcaacggt acaaaaaaga caccggcaat
    11221 caagcataag ttaatcatca tggagataag acttcgacgg tcctttgaca aaactctcac
    11281 gacatgggaa tctggtttat ttgctttcat gatttgcctc atcaagatac caaaagaaaa
    11341 actccacatc gtgtatacta agacataagc catctcaggc caaaaagctt cagggttacg
    11401 cccgacccaa ctagtaacag aaggacataa agtcagccaa aagagcaaaa agatattcga
    11461 ccagaccact cggtaattaa ttcgttctgc catctgaaat atacgatgat ggttcaccca
    11521 gatcaccgca acaaatataa agctgatggt ataagctaat aatgtatttc ggatgaccca
    11581 taaagaatga aagctagatg tattagggat cttgatctct aatacaataa ctctcaaaac
    11641 aatggctacc actccatcag taaatgcttc taatctcgac tttgtcacga tgattcctcc
    11701 tcagatgatt cagcgctttt attttatcac ttacgttatt aaataaccat cgttttatat
    11761 agggaatttt ctgaaaattg ttgcatattt ttcgaaattt tgttactttt aaaaaaaccg
    11821 taaaggagtt atgtatgaca aaaaaattat catttaaaga ctatcttttt ataggctcca
    11881 tgcttttcgg tctatttttt ggtgctggaa atcttatctt tcccgttcat ttgggtcaag
    11941 aagcaggcgc tgctaccttt tgggcgaatc taggtttctt ggttaccggt atcggtcttc
    12001 ccttcttagg ggtgattgcc attggtgtat cgaaaagctc aggtgtatat gaattagcaa
    12061 aacggataaa tcaaacatat gcgatggttt ttaccatctt gctttactta gttatcggac
    12121 cgttttttgc tttacctcgc cttgcaacca catctttcga gattggttta gcaccattta
    12181 tcgatcctag cagacagacg ctatatctag caattttcag tattgtattt tttgtgctgg
    12241 cttggtggtt ttcaagaaaa ccgacaaaga tattagatta tgttggaaag ttcctgaatc
    12301 ctgcattttt ggttttatta ggtgctttgc ttctccttgc ttttttgaat ccactaggtt
    12361 ctgtaaatca agcgcctatt cagccaaatt atcaagagca tgcttttttc acagggttta
    12421 ctcaaggata taacacatta gatgcgcttg ctgctttagc atttgggatc atcattgtca
    12481 caacaatcca aaacatgggt gtaacaaaac ctgctgaaat cgctaaagac acgatcaaat
    12541 ctggtgcgat cagcattata ttgatgggaa tcatttatac tttgctcgct tatttaggtg
    12601 ctatgagctt aggtagcttt gctctaagtg aaaatggcgg gatcacgctg gctcagattg
    12661 ctgatcatta tttaggtact tatggcagta tcttattagc atttatcgtt atattagctt
    12721 gcttgaagac cggtattggt ttgattacag cttttgcaga aacattcaca gatttgtttc
    12781 cgcaaagaaa ttacgcattc tttgttgcac ttgctagtat ccttccttgt ttagcggcaa
    12841 acgtaggctt gacgaatatc attcaatttt cgttgcctgt tttgatgttc atctatccat
    12901 tagcaatgac actgatttta ctagtgttag ttggtccttt gtttaagcag cgtacggctg
    12961 tttatcgaat gactacttat tttacattga ttgcttcgat ttttgatggg ttgaacgctt
    13021 gcccagaaac tatcaaacaa acacctattg ttcaaaatct tttatatgtt gcggaaagct
    13081 acttgccgtt tttcaaattg gggatggggt ggatcgtacc agcagttatc ggttttgtta
    13141 tcggactgat ttggagtttc gctaagaaag aagaagtagc agattgataa aagaggttgt
    13201 gaaagagacg ttcagccttg agcaataaaa agtaattttg aaaaatggct tctcacattt
    13261 ttgcataaat ttgacttaat accgagaaac tggcttttga acaccgttta ttgagcgtct
    13321 ataaactgac ctgagtatta gagaaacaaa acggcaaagt agctccatat actttgccgt
    13381 tttgttttga actaataccg aagagaagct gatcaaacaa catttctttt ataatataga
    13441 ttttagacat atatatgata taaaaatagt
//
LOCUS       DACEXQ010000053        13334 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.053, whole genome shotgun sequence.
ACCESSION   DACEXQ010000053 DACEXQ010000000
VERSION     DACEXQ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.053"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(178..735)
                     /gene="frr"
                     /locus_tag="IXH04_001127"
     CDS             complement(178..735)
                     /gene="frr"
                     /locus_tag="IXH04_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAQ1851747.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(738..1460)
                     /locus_tag="IXH04_001128"
     CDS             complement(738..1460)
                     /locus_tag="IXH04_001128"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAQ1851748.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1596..2477)
                     /locus_tag="IXH04_001129"
     CDS             complement(1596..2477)
                     /locus_tag="IXH04_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAQ1851749.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2576..3358)
                     /gene="rpsB"
                     /locus_tag="IXH04_001130"
     CDS             complement(2576..3358)
                     /gene="rpsB"
                     /locus_tag="IXH04_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAQ1851750.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3717..4196
                     /locus_tag="IXH04_001131"
     CDS             3717..4196
                     /locus_tag="IXH04_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAQ1851751.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4413..5504
                     /gene="phnW"
                     /locus_tag="IXH04_001132"
     CDS             4413..5504
                     /gene="phnW"
                     /locus_tag="IXH04_001132"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAQ1851752.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5497..5625
                     /locus_tag="IXH04_001133"
     CDS             5497..5625
                     /locus_tag="IXH04_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAQ1851753.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGTQLTDKEIR"
     gene            5629..6174
                     /locus_tag="IXH04_001134"
     CDS             5629..6174
                     /locus_tag="IXH04_001134"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAQ1851754.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6627..8318)
                     /locus_tag="IXH04_001135"
     CDS             complement(6627..8318)
                     /locus_tag="IXH04_001135"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAQ1851755.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYAFNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8738..9685)
                     /gene="arcC"
                     /locus_tag="IXH04_001136"
     CDS             complement(8738..9685)
                     /gene="arcC"
                     /locus_tag="IXH04_001136"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAQ1851756.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9800..10819)
                     /gene="argF"
                     /locus_tag="IXH04_001137"
     CDS             complement(9800..10819)
                     /gene="argF"
                     /locus_tag="IXH04_001137"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAQ1851757.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10910..12139)
                     /gene="arcA"
                     /locus_tag="IXH04_001138"
     CDS             complement(10910..12139)
                     /gene="arcA"
                     /locus_tag="IXH04_001138"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAQ1851758.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            12600..13301
                     /locus_tag="IXH04_001139"
     CDS             12600..13301
                     /locus_tag="IXH04_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ1851759.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT         3752 a         2710 c         2366 g         4506 t
ORIGIN      
        1 ttaaaagatt cccactatct taagtattat agcaatatct aactgttttt cctatgaaat
       61 cattgttttt ttatttaaaa actgaataat tttttctttt tttaattttt aaataaaaga
      121 aatataaata aaaacagagg aagcggaatg atccgcccct ctgtttttat gattttatta
      181 aacttccaat aattcttttt ctttatcagc agcgatctta tctacttctt tgatgctgtt
      241 atctgtcaat gtttgtacgt ctttttctaa tccgcgcaaa tcatcttcgg taatatcacc
      301 atttttttgt tgttttttat attcatcgat cgcatcacga cggatgttac gaatcgcgat
      361 ttttgcattt tctgcttctt ttttcacatc tttagctaat tcttttctgc gttcttctgt
      421 cagttgcggg ataactaagc gaatgacatt tccatcattg gttggactga ttccgatatc
      481 acttgccata atagcttttt caacatcttg aagaatgctc ttgtcaaaag gtgtgatcat
      541 taaaacacgt gcttctggaa tttggattga tgccatttgg ttcaatggtg ttggcgcacc
      601 gtagtagttc acagtaatac ggtccaaaag gctagcgttc gcacggcctg cgcggatttg
      661 cccaagttca cgttgaagat tttgtgctgc ttttttcatt ttttcttttg cttctgtcat
      721 aattgcatct gccattttta tttccccctt acagttgttc cgatattttc gcctaagatt
      781 gcacggcgaa tatttccgtg ttcatttaaa ttgaagacaa gcagtggaat atcattatcc
      841 atgcttagtg aacttgcagt tgaatccatg acttgtaatc ctttggcaat aacttccaga
      901 tgagtcagtt cttcaaattt tactgcattt gcatctactt tcggatcagc agaataaaca
      961 ccgtctacat tgtttttagc catcaggata acatctgcgc cgatttctgc cgcgcgtaat
     1021 gctgctgttg tgtctgtcga gaagtacggg ttgccggtac cgcctgcaaa gataacgatc
     1081 cgtccttttt ctaaatgacg ttctgcttta cggcgaatat atggctctgc gatttgacgc
     1141 atttcaatcg aagtttgcac gcgtgtcggt acacccacgt tttccaacgt atcctgtaaa
     1201 gctaaggcat tcataactgt agctaacata cccatgtaat cagcttgagc acgttccatg
     1261 cccatttgtg ctccgatttg tccgcgccag atatttccgc cgcccacaac gattgccatt
     1321 tcaacgccca gctcgtgaac ttctttgatt tcttggatga tttctttgat tactggtggt
     1381 ttgattccaa atcctgcgtc cccagctaaa gcttctccac tcaattttaa gactacacgc
     1441 tgatatttcg gtgtgaccat gttcattcct ccaatatttt ctattgccat tttaccatac
     1501 ttagatgatt tcgacactct aagactggtt tttcttgttt tgcttaaaaa aagggagcgc
     1561 acaaaccgtg cgttcccgtc tgttattcaa cagaattatt ttttcacttg gctcataact
     1621 tcttctacaa agttatcttc gcgtttttcg ataccttcgc caacttcgaa acgtacgaat
     1681 gttttaacag ttgcgccttt tgatgcaacg tatttttcaa cagtcatatc aggatcttta
     1741 acgaatggtt ggtccaccaa agcgatttct gctttgaatt tcttcaagcg tccttctacc
     1801 attttttcaa cgatattcgc tggtttgcct tcgttcaatg cttgttctgt aagaacagtt
     1861 ttttcatgtt ctaattcagc ttctggaatt tgtgattcgt ttacataacg aggattgata
     1921 gctgctacgt gcatagctac gtctcttgca actgtttcgt cagttgttcc atctagaaca
     1981 gtcaatacgg caatacgtcc gcccatatgc aagtatccac caaatgcagc attgtcgtct
     2041 ttttctacaa cttcaaagcg acggaagctg attttttctc cgataacttg agttgcttcg
     2101 atcaagtcag attcaattgt acctttatct gttttgattt tcattgcagc ttccatgtct
     2161 gctggtttgt tttcagcaac aagttctgca atttctttta ctaaatcttg gaacatttcg
     2221 tttttagaaa caaagtctgt ttctgagttt acttcaacga ttgctgcagt gttacctttc
     2281 acagcaactg aagccaatcc ttcagctgca atgcggtcat ttttcttcgc agcctttgcc
     2341 attccttttt cacgtaaaag atcaactgct ttttccatgt cgccttctac ttcaactaat
     2401 gcttttttcg catccatcat tcctacgcca gtcatttcgc gtagttcttt taccatttta
     2461 gctgtaacgt ctgccatgtt taactcctcc tgtgtgtttt tctttttaaa aaaagctgct
     2521 tcaaaggaga gtgaggcttg tcgcccagcc tttgaaacag ctccatcatt caaaattatt
     2581 ctgctgaaga attgtcgcct tcaacaacat ctacgatttc ttcgattgat gttgctgtgt
     2641 tttcttctgt gaaatcttct tcaactactt gatcttcacc ttggtttcct tcgatgaaag
     2701 catcagccat ttttgaagta atcaatttaa cggcacgaat tgcatcgtcg tttgaaggga
     2761 ttactacgtc gatttcatct ggatcgcagt ttgtatcaac catagctacg attgggatat
     2821 ttaatttgtg tgcttcttga acagcaatac gttctttacg tgggtcaact atgtacatta
     2881 catctgggat tcttggcata tcagcgatac cgcctaagaa tttttcaaga cgttcacgtt
     2941 ctttgttcaa accagcaact tcttttttag gtagaacttc aaaagttcca tcttcttcca
     3001 ttgcattgat ttgtttcaaa cggctgatac gtttttggat agtatcccag tttgtcaatg
     3061 ttccgcctaa ccaacgatgg ttaacgaagt attgtcctgc acgagttgct tcttctttga
     3121 ttgcttcttg tgcttgtttt tttgtaccta cgaataatgc tacgccgcct tcttcagcaa
     3181 cgctcttcat gtaatcataa gctgcatcta ctaatttaac tgttttttgc aagtcaatga
     3241 tgtagattcc gtttctttct gtgaagatat atttcttcat ttttggattc cagcgacgag
     3301 tttggtgacc aaagtgtacg ccggcttcta gtaattgttt catagaaatt actgccatgt
     3361 ttgtttcctc cgatttggtt ttatttttcc tcttcttatc ttctaccttg ccggcaaact
     3421 ttttcaagca cctaccgaca atccaataag aatgtggatt tgctgatgca taaagcaccc
     3481 tcagctatta tacagacaaa agatccgtat cgcaagagca aatatacttt tctgctcaac
     3541 attcaatagt ttacctgaaa gataaaaaga attcaagaaa aacagtagat ttctctttgt
     3601 ttctttatta tcctttttcc ataaaataat tttaggtata aaagacgaat attattgggc
     3661 aatccttctt ttttttgtta tgatacatgt gaaatctttc gtaaaggaga aagaaaatgc
     3721 gcaaagctca aagacatttg ctgattaaac aagtcattga agaattcccg atccgtacgc
     3781 aagaagaact attaaacaaa cttgggcatt atggcgttac cgccacgcag gcaacgattt
     3841 ctcgagatat ccgtgatcta aaaatcgtga aggcacctga tgaaaatggc gtttcccgct
     3901 tcgtattgtt tcgcgaaaaa ggaactcctg agactaggac tgaggaagag gatcggctgg
     3961 ttcaaatgat tgaagacatt gtattgaaag tcgaacgagt ccactttttg acgatcgtca
     4021 ataccttgcc agacaatgct catttatttg catctgtttt agatgagatc aaacctccct
     4081 ctattgtcag tacaattgct ggattcgata cgatcatggt catctcggaa aatgaggagg
     4141 acgcaaaacg agtagaagaa tttcttcacc atccaactga agtatcactt ttttaatcag
     4201 agcacgtatt cctaatatgg aatgcgtgtt tttttatttt caatattttc cttattgaat
     4261 aaacctttac actctttaca aaaatacatc attttttatt caaatttttc aacaccttga
     4321 ttgaatcaca ataatcatca tttatattgg atgttaacaa gagcagcttc ttgctctttg
     4381 tgtatttcaa aaaagaaatg aaaggaaatc atatgtataa attattaact tctggaccat
     4441 tgacgacttc tgaaacagtg aaaaatgcta tgctgatcga tcattgtact tgggatcaag
     4501 aatacaaaga aattacacaa tggataagaa aaaaattatt ggatcttgct caagtatcgg
     4561 aaggaatata cacaacggtc ttaatgcaag gtagcagctc cttcggggta gaatcggtat
     4621 taagttctgt gatcaggcca gaagatactg tactgatttg ttcaaacggc gcatatagac
     4681 aacgaatgat caacatgtgc gaatgtctga aactgaacta taccgtttat gaggtatcgg
     4741 aacatcaaat cctccaagct catgtgatta cacagctgat tgaagagaat ccgtctatct
     4801 ccgcactagc aatggtccat agcgagacta cttccggtat tttgaataat ttggaagaga
     4861 ttgctgctgt cacacaagaa agagatcttt tgtttatagt ggacgcgatg tcaagctttg
     4921 gtgggatacc gatttgtgta gccgaactag ggattgattt tttgatttcc agctcaaaca
     4981 aatgtattca aggtgttcca ggcttttctt ttgtacttgc taataaagcg gaactggaaa
     5041 aacgaaaaaa tgttagccga agtttgtcac ttgatctata tgaccagtac cgcgtgatgg
     5101 aaaacgagaa tggcaaatgg cgtttcactt cccctaccca tacggttttg gcttttcaac
     5161 aagcgctgaa ggaactagag gcagaaggag gaatcgaaac tcgatattca cgttatctcg
     5221 ccaataatca tgtgctacga caaaaaatga cagagatggg gttccagcct ttcattcaag
     5281 aatatcaggg gccattcatc acctcttttc tttaccctaa ggactggcga tttgatttta
     5341 atcattttta tcagtattta aagcaaaatg gatttgtgat ctatcccggg aaaatcagcg
     5401 atcatgaatg ttttcgtatc ggaaatattg gcgagatcta tccggaagat attttggaac
     5461 tgacagcaat tatcaaagac tataaggaga acgttaatga ttaaaactgt aatttttgat
     5521 tgggcaggaa caactgtgga ctttggctgt atggctcctg tccacgcttt tagaaatgct
     5581 tttctagaaa aaggaaccca actgactgac aaagaaatcc gctaaccgat gggaaaatta
     5641 aaatgggatc atatccaaca gcttctttcc ctcccttctg tcaaaaaaca atggatagct
     5701 atttatggac aactacctca ggaaaaagag gtggaggaat tgtatcaaca atttgaacat
     5761 tatctgttta aagatttggt caagcattct accttgaaac ccgacacatt ggaaacgatc
     5821 gaacggttga gaaacgctgg gatcaatatt ggttccacga atggatttaa tgctcctatg
     5881 atgacaatcg tagcagaaac cgcaaaaaaa gaaaggtatt caccagactt catggcaact
     5941 tttgaagacg tcgaagggta tggtcgaccg tttccttata tgatccataa aaacatgcag
     6001 tacttcaaaa acaagtcggt tgatgaagta atcaaagtcg gagatacggt ctcagatatc
     6061 caggaaggga aaaatgccgg agtgttgaca gtaggtgtaa ttgaaggtag ttcgctaatg
     6121 gggatttctt atgatgagta ccaaaaatta gagatgggga aaaagatcgt ttaaaacagt
     6181 ctgttgctct tagattccaa caagccggcg ctgatatttg tgtaaacaat ttaaaagagt
     6241 taacagaata cattttgtac gctgacaaga atccagaatg acttttatac aaaataagag
     6301 gttgtgaaaa agctgtcctg tatcaagtaa taaaaacagc caagcaaaaa tagctcctca
     6361 tatttttcgt ttggttaggc ttattaccgc agatgcaagc ttttgaacac cgtttattcg
     6421 aaaaaaaaag ggagctgtga cacgactttt gtcacagtcc ccttatttgc aaacaaacag
     6481 tgtttaagag acaacctctc cactaaacgg tgttcattca gctgactctc ggtatttgct
     6541 caaaaaacga taaaatttga aaagctattt tctcttttgt ttctctaata ctcggatcag
     6601 aacgctgatt tcacattctc tttttcttac atcttatctg gtgcatgaag tcctagcaaa
     6661 cgaaggtctt ctttcaataa tacagtcact gcataaacta gagctaagcg tgcttctttt
     6721 tgttcatcat cagctaaaat cttcgtatga gcatagtatt tgttgaatgc ttgtgctaat
     6781 ttgattgcat gtttcgcaat cactgacggt tcgtattttt ccccagcaga caagaccgtt
     6841 tcagggtatt tttgaactaa tttaaccact tcccagctgg tatcgtcatt aaatgcatag
     6901 tctgctgttt ctgaagggac aaatcctgct ttttccaaca ggctgactgc acgagcatgt
     6961 gtatattgca cataaggtcc agtttctcct tcaaatcgaa caacttcttc caggttaaag
     7021 tcaaatgtat tcagtcgatc gtttttcaaa tcgtggaaaa tgactgcccc aaccccaact
     7081 tgtttagcta cagcatcttt gttttccaaa tcagggtttt tctcgctgat ttgttcttta
     7141 gcagattgga ttgcttcatt taatacttct tctaataaga cgatctttcc tttacgagtt
     7201 gataattttt taccaccttg cgtgatcaaa ccaaatggaa tatggtgcat gtcgtcagac
     7261 cagtcaaagc ccatttcctt caagacagct ttcaactgtt taaaatgata gctttgttca
     7321 ttccctacta catatagaga ttgtttgaag tcgtaagtac gtttacggta caatgcagca
     7381 gctaagtcac gtgtaatgta tagtgtcgca ccgtctgact tcttaatcaa cgctggattc
     7441 aggtcataag cagataggtc aacgatctct gcacctttat cctcgttcag caaatgtttt
     7501 tcttctaaaa gtttgacgat ttcatccatc ttgtcattgt agaaagcttc accgttcaac
     7561 gaatcgaaac gtacttccag cagatcatag attttattga attctttcat cgattcatca
     7621 cggaaccatt gccataattg aatagcctct ttatcgcctt cttccagacg tttgaaccaa
     7681 gcacgagctt cttcatttaa ttctggttct gtttcagcta cttcatggaa ttggacgtat
     7741 aaacgaagca attcattgat tggttctgct ttaacagctt cttctgtacc ccattttttg
     7801 taagcgacga tcagttttcc aaactgtgtt ccccagtcac ctaaatggtt gatgcggatt
     7861 ggctgataac cgattttttc cataatgaaa ccgatcgaat taccgataac tgtcgaacgc
     7921 aaatgtccca ttgagattgg tttggcaata ttaggagaag acatgtcgat tggtactgtt
     7981 ccttgattcc cgatgttgct atccccataa tgttcttttt ctttcactac tgtttgcaaa
     8041 acttttttgc tgataagttc tttattcatg aaaaagttca agtaaggtcc gacaacttcg
     8101 attttttcaa agttggcaga atcgattttt tctgctaaat ctgcagcgat ttgctgtggc
     8161 gcttttcggt aaacttttgc taatgaaaag gctggaaatg caacatctcc gtgttcagct
     8221 gattttggat tttctagtaa ttgctcgact tgttccagtg ttaaatcatc tttgacagcg
     8281 tcatagacag cttttgcgac aagatcttta ttattcatct ttattcctcc taaagtgttc
     8341 ccttgattgt taaacaaaaa aaatctctag cgacggcata cttagtatgc catcactaga
     8401 gacgagattt cccgcggtac cactccaatt gtcctaaaaa aggaccagct ccatctgata
     8461 tcggtagacg accgtctgtt taaaacagaa ttctccaagt gcgcttcgtt gtatcttctt
     8521 cattcggctt ccaccacttc cgaattcgct agccaagaat gatacaagta ctctcttgtt
     8581 caacaaatct tagtcttatt aaactttctt gaattatatc agaaaaaaaa acgaaaggct
     8641 agtcgtttca taaaaaaaaa atccagatgg cctaaaggag aagaaatacc acctggatag
     8701 ctataaatca tttcgtaaat acgaaagact taacaaatta atcagctacg ataatcgttc
     8761 cttcttcaga agctaaaaga ttctcgatat tttctaaaga agtgatgatt gccttagcgt
     8821 ttggttttgc ttcaacaaat gcaatagctg cttctacttt cggaagcatg cttcctggag
     8881 cgaattgctt ttcatcaatg tattgtttca tttctgaaac agtaacagtt tctaatttct
     8941 tttgatctgg tttttggtaa ttgacataca cattgtctac tcctgtcaat acgatcagca
     9001 aatccgcatc gatgatttcg gctaattttt ctgaagcaaa atctttatcg attacggctt
     9061 ctcgtccttc taatccagta gctgtttcta caacaggaat cccaccgcca ccaacagaga
     9121 ctgtgataac tccttgatcg accagcgttt cgatgacacg tgcttcttta atcgagatgg
     9181 gtttagggga agcgactact tttctccatc cacgtccagc atcttctttg aatgtcacag
     9241 tggagtcagc attcatttgt tctttggctt cttcttccgt gtagaatgga ccgactggtt
     9301 tacttggatt tttgaatgaa ggatcatttt catccacaat aacttgagtg actaaagaaa
     9361 ccacatcttt atcgatccct ttttccttca gtacttcgcc catggcgttt tgtaaccagt
     9421 aaccgataga tccttctgtc attgcaacac atgtatctaa aggcattgcc ggtgttttct
     9481 cagaatcagc agcttgctgc tgaatcaata ggttaccgac ttgcggacca tttccgtggg
     9541 agatgatcaa ttcatctcct tgttcgataa atttcactaa atactttgct gtttccatta
     9601 acgcttcttg ttgggctttt gcacttgcat ccgtagacaa gatggcattg ccccctaaag
     9661 cgacaactac ttttcgtttg gccatttcat tcgtcctttc ttcagttcta ccgaattttc
     9721 tagataacaa aacgagccag cgttttgtga ttttccctca atgctgactc gttttttcgt
     9781 gagatgaatg tggttatgct taaacgcgag ggataaacaa gttacctaat gttgctgcca
     9841 tgatcgcttt gattgagtgc atacgatttt ctgcttggtc aaattgacga gcatatttgc
     9901 tgcggaagac ttcatctgtt acttccattt cagtgatgcc aaagcgttct ttcatttgtt
     9961 caccgtaaac agtgtttgta tcatggaaag ctggcaagca gtgcaagaag atcaaacgat
    10021 ctgtgttgtg tgttttttct accatttcca tattgatttg gtaaggtttc aacaatttga
    10081 tacgttcttc gaatttatct tcttcaccca ttgataccca tacatcagaa taaaggacgt
    10141 ctgctccgtc tacaccttta tcaacatcgt cggtaatcat caattgtgca ccggattttt
    10201 cagcaaagcc ttctgccatt ttgacgattt cttcttcagg ttgtaattct tttggtgcaa
    10261 cgatacgcat attcgctcct aaaattgcac ctgtgactaa taaagagtta gccatattgt
    10321 ttcgaccatc tccgcagtaa gcaacagtga taccttctac tgttccaaag ttttcttgga
    10381 tagttaaaaa gtcagcgatc atttgtgttg gatgccattc atcagtcaag ccattccaaa
    10441 ctggaacacc agagaattct gctagttcct ctaccatttt ttgactgaat ccacggaatt
    10501 cgattccgtc aaacatacgt cctagtactt tagctgtatc ttctgtggat tcttttttac
    10561 ctaattggat atcatttgca cctagatatt caggatgtgc acctagatcg atcgctgcag
    10621 tagtaaatgc agaacgagta cgtgtagatg ttttttcaaa taaaagcgca atattttttc
    10681 cttctagata gtgatgtggg ataccacgtt ttttcaagtc tttcaagtgt tctgaaaaat
    10741 cgatcaaata ttgtaattct tcttttgtaa agtctttttc tgctaataag cttcttcctt
    10801 ggaatactga ttctttcata ttaaacattc tcctttagat tgtttttatt taagattaaa
    10861 gaggaaatcc cgacggcagc tgacagtaag actgccgtca ggaaacaaat tacttcaagt
    10921 cttcacggac aagtggttgg ctcatgcagc gtggaccacc acgacctcgt gacaattcac
    10981 ttgaattgat ttctaacact ttgatgccat agctacgaag caattcgttc gatacataat
    11041 ttcggttata agtgactaca acacctggag cgattgccaa tgtgtttgat ccatcattcc
    11101 attgttctcg aggtgcaacg atctcatctc cattaccagt tgggatcagt acaagatcat
    11161 ctaatccaag agcagctttc atcgtttcgt gtaagttatc tgaatgagta atcttgatat
    11221 catcaccatc tggttcgatt gtaaagacat ccatcttacc gtctttactt tgaattgctg
    11281 gatggatagt gaatttatcg tgatctacca ttgtaaatac ggtatccaaa tgcatcattg
    11341 cacggttgtt cggaattttg attgccaaca ctttttcaaa tcctgaattt tttgcgaata
    11401 ggtggcgtgc taatttttct aaagctttcg cattagttcg ttgagaaatc ccaacagcta
    11461 ctactttatc gcttaagatc aattcgtctc cgccttcgat atgatctgga ttttcacgat
    11521 ccaaccatac ttcgattcct ttatttgcaa atcttggatg atgtttcaaa atatattctg
    11581 taaacatcga ttcacgacga cgtgcttcaa aagtcatctt attgactgtc atcccatttc
    11641 ccattgatgc ggaagggtcg cgtgtaaagt acaagtttgg cattgggtcc atgtaaaatg
    11701 gatagtcatc gtctgctgac agatcatata gactgctaga acgtacttcg atatcttttg
    11761 tacgaacccc agccatgatt ttatctacca tttcttgcgt ttccattgat agaaggaact
    11821 catgaagacc ctctcttaca gcattcgatg caatatgaga ttcatccaac attttattca
    11881 agaattgttc tttgacattg cctgcgtcaa tcgcttctgc cgctaatttt tccaagtaaa
    11941 gagtctcgac gccttcattt tgcaatgtct tcgcaaagtt gtcatgttct tcttgagcaa
    12001 tcggaagata tggaatatca tcgaacaaca gacgatccat aatatctggt gtcaaatttt
    12061 ccacttcttg tccaggccgt ttcagtaaaa ctgtctttaa tttccctatt tcagagaaaa
    12121 cgtgaatagg tttatccatg ttgtgttgcc tccttctttt tgattacatg tataggatat
    12181 cctccttttg taatcacttt cataattttg tctcattcaa aatgagacat tcctcacatt
    12241 aatattcgat aattaaataa ttaaacgttt ttctattttt aagcgtaatt tattcatttt
    12301 ttattctgta tacactttca aaaaaatatt tattcattga aaaaattttt ttgaataaat
    12361 taaaaaaata aattatatta tcgttgttag cgtttactat atgtattatt gttgtttttt
    12421 atacacaaat cattcgcttc aaaacaaatt attcaatacc attgtatatt ttttacataa
    12481 taacaaatta tatttgtaaa gtattaaaac ttgtttctaa tgttggtatg ttaaaacttt
    12541 acaaattctt taaaaaaagt gacaataaat atagtgaaca ataaggagag gactttttaa
    12601 tgcgtgttaa taaaatcgat tttcactttt caaaactcag atatcaacct gattttgaac
    12661 atttctcgga tgaagagttc caacagatca aggaacatac tgttcttcgt tcttataaaa
    12721 aaggacagat ccttttcgac gatggtgatc ccagacaacg gttgtatatc ttgaccaaag
    12781 gactcgttcg tttagaacgt tatgatgaaa gtgcaactta ttactactat gactatgtga
    12841 atacaaataa cctttttcct atgtcaggaa tgtttggaga agagacctac acttatacag
    12901 ctcaagcgat gactgatatt gaaacattct accttcctgt ttcctactat gaaaaattag
    12961 cacgaaaaaa tcccgaacag ttgatctata tgatacaaaa aatttctaaa gtagttgaaa
    13021 tacatgaatt gcgtatccaa atcggattga cctctagcgc ctttgaccgg gtaaaacaaa
    13081 atttattcat tttgaaagaa gaattgggcg taccaacaaa agatggtcat atcaccattc
    13141 cttatccgat caccttaaaa gaattagcag tgaatagcgg cacgacacgt gaaactgctg
    13201 gacaagtaat caaacaacta cgtgattccg ggctgttaga ttatgacaaa aagcgtttta
    13261 ctttctgtaa gacagctact actccagcta tgagagaata acgcgaacaa aaataagcag
    13321 tgaccctgat aaat
//
LOCUS       DACEXQ010000054        13128 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.054, whole genome shotgun sequence.
ACCESSION   DACEXQ010000054 DACEXQ010000000
VERSION     DACEXQ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.054"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            1067..1741
                     /locus_tag="IXH04_001140"
     CDS             1067..1741
                     /locus_tag="IXH04_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ1851760.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTVGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIVYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            1738..2079
                     /locus_tag="IXH04_001141"
     CDS             1738..2079
                     /locus_tag="IXH04_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851761.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLILEAK
                     KRRIKSFFAQF"
     gene            2109..2468
                     /locus_tag="IXH04_001142"
     CDS             2109..2468
                     /locus_tag="IXH04_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAQ1851762.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            2992..5073
                     /gene="cadA"
                     /locus_tag="IXH04_001143"
     CDS             2992..5073
                     /gene="cadA"
                     /locus_tag="IXH04_001143"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ1851763.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            5384..6850
                     /locus_tag="IXH04_001144"
     CDS             5384..6850
                     /locus_tag="IXH04_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1851764.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            7465..8004
                     /locus_tag="IXH04_001145"
     CDS             7465..8004
                     /locus_tag="IXH04_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HAQ1851765.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
     gene            8078..8518
                     /locus_tag="IXH04_001146"
     CDS             8078..8518
                     /locus_tag="IXH04_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAQ1851766.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            8531..8740
                     /gene="copZ"
                     /locus_tag="IXH04_001147"
     CDS             8531..8740
                     /gene="copZ"
                     /locus_tag="IXH04_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAQ1851767.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            8740..10926
                     /locus_tag="IXH04_001148"
     CDS             8740..10926
                     /locus_tag="IXH04_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAQ1851768.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            10946..13117
                     /gene="copB"
                     /locus_tag="IXH04_001149"
     CDS             10946..13117
                     /gene="copB"
                     /locus_tag="IXH04_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAQ1851769.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
BASE COUNT         4398 a         2210 c         2875 g         3645 t
ORIGIN      
        1 gaatttatcc ataagaggtg atgaaaaagc tgttctgcat caagtaatta gaacagccaa
       61 gcaaaaacag ctgttcatgt ttttgaattg gcgggactta ttaccgcaga tgtaagcttt
      121 tgaataccgt ttagtggaga ggttgtgaaa aagctgttta gtttcgagta ttaagaaaaa
      181 ttttgtggaa atggctttcc acatttttgc ataaatttgg cttaataccg agaaactggc
      241 ttttgaacac cgtttaagga agaggttgtg cgcctgccgt tcagctccga gtcacaagga
      301 gtaatttcaa aaaacagttt ttcatgtttg atgactattg tgacttgtga ccgaggagtt
      361 ggccggagag caccgtttat tctaaaaaag aactgcgaac tctcgcagtt ctttttttaa
      421 aatacgggat aaaggaagtt tccagcagac aagagaagaa agcaaaagaa attgctattt
      481 gattcacaag gttctataac gaaatcgcaa tcaatcaaac atttacaaag aaggtaattc
      541 aaatggaaat ctgatttgat gatgcttctg ctgttgaaat aaagcagcat ctagcacctg
      601 aaaacaaact aatagtaaca ttcgaagatg gagtaggtcc gtattttcag catgcaataa
      661 tccatatgta agttcaattc acccttgaaa tcatcggacc agataaagtt atgaccaatc
      721 tatcccaagc aatctagaag agatattagt aaagggacat ttactagaag tcttagatga
      781 gcctataaat atatgttctg atggcgtcat cagtggtctg attgacggaa gcttaggtgt
      841 gatcgatctc tctggaccaa acaacgaagg aatcaagggg aatccggcac gttaagaaaa
      901 aaatagcata aaaaactaga cacttgatga gcgttcattc aattgtttga agggactagg
      961 ctggtgtcat tttttataca tatattttct ctttcgtctt gatagggctt atgggataat
     1021 ataaggaaga aaatcatcgt tttattcaaa aaggagtttt tgacaaatga aaaaacgtat
     1081 attatttatc ggtgatagca ttacagacgc aggaagagac cgagcagaag caactgatct
     1141 aggtaaaggt tatccacttt tgattgccgg aaaactaatc tcccaatatc caacgatgga
     1201 atttgaatgc atgaaccgtg ggataggagg agacaaaatt gcagatctgc aagtacgctg
     1261 gcaagaagat tgtttagctt tacaacctga ctatgtatcg attttgatcg gaatcaatga
     1321 tacctggcat aatactagcg atcaggctat attcggaact gagaaatctg ctcagcaatt
     1381 tgaatccgtt tatcgaaaat tactaaatga acttcaaaca gtgggaatct gcaacatcat
     1441 cttgatggag ccgtttgtac ttcctataac cgacgaccgt catggctggc gtaaagactt
     1501 agaccctaaa atccaaattg ttcgtaaact tgctaaggaa tatggcgcat tgctggttcc
     1561 tttagatggt atattcaatg ctttagggat tgtttatggt ttttcgaaat atacaagaga
     1621 agatggggtc cacccaacga ttggcggaca tgagatcatt gcacaaagct ggatagatgc
     1681 aattgccgca gtcgattttt ttagtggaga taaaaatgag cgaaagaaaa gtgagaaatg
     1741 atggaaatac ggtgggcagt aaaagaagat ctacctcaaa tcaatcagct gattactgat
     1801 catccagaat cattcgtaaa agcaaaaacg gcagctgacc taaatgataa gaataaacta
     1861 ttgctcgttg ctgtaacaaa agaaaaacgg attatcggat ttgcgcaagc ggacgtgcaa
     1921 cagtcagaag aacgattgga tcagcgacca tcaggaaaac taatccaaca cacattagta
     1981 acaaaagaaa atacggaaga aatccaaaaa aaatttgcag aatatttgat actggaagct
     2041 aaaaaaagaa gaataaaatc attttttgcc cagttttaat caaaaacagt aatagaagga
     2101 gaaatacaat gagttgttta ggaaatgtca tctggtttat attcggcgga ctaatcggtg
     2161 gtcttagctg gttagcagcg gggatacttt ggtgtatcac gatcataggt atcccaatcg
     2221 gtatgcaatg ttttaagatc gcctcccttt cttttgcgcc ttttggcaaa gaagtaattt
     2281 atcaccagtc tggcatgtca ttgattgtta atatcatctg gctgatcgtt tcaggcctac
     2341 caattgctct cgcacatgtc gtcagcggca tccttctatg tatcacgatc atagggatac
     2401 cttttgccaa gcaatctttc aaacttgctc gattggcgtt gatgccattc ggtgcagaaa
     2461 ttgtataaaa gagaataata aaggttagat aaagcataga gtcaggtggg atacatgtat
     2521 ttcacctgac tctatgcgtt ctcctgtata agttcttcca tgtgactttt acgtctttta
     2581 tttatatggg gttactgttt atggaaagtt gaaataattt tcattaaaga actctttcta
     2641 tatttttgaa agcgtagtgg agcttcgaat atctgggaag gacattttgc agggattgaa
     2701 aaaagatatt gtcctatcga aaaattaatg aatttagaca aattgccagc aacaggcttc
     2761 aaacttagtt gctatcctgt caagatcaag aaggcaagtg cgggttggat aagagcagtg
     2821 gcaatgatcg aaaagaaaaa gaaagaataa gccaaaacat ctgtatggaa gaaattttcc
     2881 gatgcagatg tttttcattt tttctttcgg ataccttgac atatgaataa ccgttcattt
     2941 ataattagtt atgaagagat attcatatgt tgaggcgagg aggtcattaa aatgaaaagt
     3001 tacagattag aaggtttaga ttgtgcgaac tgtgcaatga aaatcgaaaa aggagttcag
     3061 aagatcaatg gagtcaagga agcaacggta aacttcactt ctggaaaact aacgattgac
     3121 gcagaggaag atcatttggc tacgattgaa caagaaataa aaaaagtagt gaaggaatta
     3181 gaaccagatg tcaaagtaac agaaatagat aaggaaaaag tatctgaaca tggaaatgaa
     3241 aaagagagaa atacgctgtt ccggatttta ttttcccttg caggtatcgc tttattgcta
     3301 ttatttgatt ttaatgaacc aattcgcttg atagggtatt tattgattta tctgctgata
     3361 ggatatgatg tagtgaagaa ggcagtgatg aatatcgtca aaggaaaaat ctttgatgag
     3421 aattttctta tgtctgttgc tacgattggc gctatgttga tcggtgaata tccagaagcc
     3481 gtagcagtga tgctatttta tcaaatcggc gagtatttcc aaggattggc agtcagtcat
     3541 tcaagaaaat cgattcgcga attgatggcg atccgtccag aaacagctca cgtgcagacg
     3601 acggaaggat tgatgaccgt gaaccctgaa gacgtgcaga tcggtcagtt tgtacttgtg
     3661 aagcctggtg aacgtgtacc gttagatgga acgatcatcg aaggagaatc attagtcgat
     3721 acgtcagcac ttacaggaga atcagtccct aaatctgtat atgttggtga aacagtatta
     3781 agtggattta tcaataaaaa taaaccattt ttagttcaag tagaaaaatc ttatgaaaat
     3841 tctactatca gcaaattatt agaattagta gaaaatgcca gcagtaaaaa agcaccagct
     3901 gaaaatttca ttactaaatt cgcgcgctat tacacaccag tcgttgtagg attggccgtt
     3961 ttattagctg ttttacctcc acttgttgtt tctggagcgg cgttttctga atggatctat
     4021 cgagcattga catttttagt gatctcatgt ccatgtgcat tagtgatctc cgtacccctt
     4081 agtttctttg gcggtatcgg aggagcaagt aaaatcggtg tattagtaaa aggttcaaat
     4141 tacttggagt tgcttgcaca aactgagaca gtggtattcg ataaaaccgg cacattaacg
     4201 aaaggtgact tttcgatcca gacgatcgat acaaaaatcg atccaaagat attcatgcaa
     4261 tatgttgcga gtgcagaaca gttttctaca catccgattg ctcaatctgt tttagaaggc
     4321 tacgcaggtc cgcttttacc aacagctaat atccaagaat ttgcaggtga agggatctta
     4381 gctgaagttg atggcaaaca agtattagca ggtaatcaca aattgatgga aagatttgaa
     4441 atctcttttc cttcaagtca agaaatcggt acattgcttt atctagcgat cgatcaaagt
     4501 tacagtggtt atcttgttat tgcagatacc ttgaaagaag atgccgtaga cgcattggtt
     4561 caattaaaaa aggctggtgt gaagaacaca gtcatgttga cgggcgattc gaaaaaaatt
     4621 gctgatcata tcgggaagca agtaggcgtg gacaagatat atagcgaatt gcttccagaa
     4681 gataaagtgc agcggttaga ggaaatctta caaagaaaca ataaaaagac cgcatttgtc
     4741 ggagatggaa tcaacgatgc accggtgcta gcaagagcag atgtaggaat cgcaatgggt
     4801 ggattgggaa gcgatgctgc aattgaagca gcagatgtag tcatcatgaa tgatcaacca
     4861 tcgaagattg cagaagcaat ccatttagca aagaaaacat tgaaaatcgt caaacagaac
     4921 attgtctttg ctattgggat aaaaattttg gtacttgcct taggtgcttt tggttttgca
     4981 tcaatgggag atgcagtatt tgcagatgtt ggggtgacag tattagctgt tttgaatgcc
     5041 atgcgaagct tgcatgtaaa aaataaacgt tgatcaatga agaggacgaa tgaacgtcct
     5101 ctttttttta tatccttttc ttaatgaaaa atgtaaagaa aatagatagg attataaaat
     5161 aactaatgtt tttaattttg cagaaaattc aaaatagctt gaaattcaat aaggtaaatg
     5221 aaataaaaga aaataactta tttttttgat ggaaaaaata tgaaaaaacg atgtgtatta
     5281 aataaaagaa tacacacata aaataacact gtcttgtaag tttgttaaaa attgaatatg
     5341 attacatctg gataacaaat ttttatcata aaggagagaa cgaatgaaca acaaacaatt
     5401 acgctggttt actgttgcgt taattgcatt taacatggtt tggggcatgg ggaatgtagt
     5461 taataactat gctcagcaag gagtatcagt tgtcacctct tggctattga ttttggccat
     5521 ttattttatt ccatacgcat tgatcgttgg acaattaggg tcagccttca aggatagtaa
     5581 aggaggcgtc agttcatggg tcgaaaatac gacatctaat aaacgactgg catactatgc
     5641 cgcttggact tactgggtcg tccatattcc ttatttggca caaaagcctc aagcaatcct
     5701 gattgctggc ggttgggcag tccaaggaaa tggaaatatc gtaaatacga tgtcagtaca
     5761 aatggtagca gttatttcac ttattatatt tcttgccttt ttatatttat caacaaaagg
     5821 actttctact ttgaaagtga ttggcggact ggccggaaca gcgatgttcg tcatgtcgat
     5881 cttatttatt cttttagctg taacggcacc gttcatcaat ccaacgatgg aatttgccac
     5941 accacatatg aatcaagtga aaacatatat tccaacattt gatttcagtt atttcacaac
     6001 cgtttcgatg ctagtatttg cagttggagg agcggaaaaa atatcccctt atgtcaactc
     6061 tacaagaaat cctgcaaagg aatttcctaa agggatgatc ttcttagcag caatggtagg
     6121 gatttgtgct gtattaggct cagtcgcaat gggtatgctg tttgctagcg ataatattcc
     6181 taaagacttg atggcaaatg gggcatacag tgcttttcaa attttaggta attactatgg
     6241 tatcggtaac tcattgatga tcatttacgc attgacaaac atgattggac aaatctccgc
     6301 attagccttt tcaattgatg caccactaaa aatcttgtta gcagatgcgg atccagaatt
     6361 tgtaccaaac tggctacgta aacgtaatcg taaaggtaca ttggtgaatg gttatcttct
     6421 gacaggtata ctagtaggaa tcattatttt gttgccgatc tttggtatca aagatatgaa
     6481 tgaattagta aaatggttga cgaacttgaa ctcagttgtc atgccaatgc gttacctatg
     6541 ggtgttcttg gcatatatga tgttgaataa agcttataag aaattcaatt ctgaatataa
     6601 atttgttaaa aatccaaaaa tcggttttat ctttggtgct tggtgctttg ccttcactgc
     6661 gtttgcatgt atcctaggaa tggtgccaaa agtagattat actgctgatc ctaaagcatg
     6721 gtggttccaa ttattcacga atatcatcac accaattgtc ttgatcttat taggtatgat
     6781 cttgccagca attgctcgtc gagacaaaaa caaagaaatc actgaaccag aaacagtggt
     6841 tcagccttaa aatagaaata cttgttacaa gaggttgtga cagaagtggt cagcttcaag
     6901 aaataagaag gaatttacga aaattgcttc tcaaattttt gtgaagtttc ggcttgtttt
     6961 tcgcagaagc tacttctgga acaccgtgaa tcagaggttg tgaaaaagct gtcctgcatc
     7021 aagtaataag aacagccaaa caaaaatagc ccctcatatt tttcgttttg ctgggcttat
     7081 taccgcagat gcaagctttt gaacaccgtt taagaaagag gtagtgagcc tgccgtttaa
     7141 ctccgagtca caaggagcaa tttcaaaaaa caacttctca tgtttgttga ctattgtgcc
     7201 ttgtgaccga ggtgttggca ggagaacacc gtttaaggaa gaggttgtaa aatcagcgat
     7261 ttgacctgag tattagagga acaaacgaaa aaatagctcc tcatattttg ccgttttttg
     7321 agttaatgct gaaggtcagc tgattgaaca ccatttattc gaaacaaggg actttgacac
     7381 gacttttgtc acagtccctt gtttttttgc ggcgttataa gtggtatgat cgaaccaaat
     7441 tccaaagaga agtgagggag aagaatgaag atcatcgtga ttggacaaag tgggtcggga
     7501 aaatcgaccc tcgcaaggaa gatcaaagaa atcaccgact ttccgctact gccgctagac
     7561 cttttatggc atacaacaga ctacagtatt caggcaaaaa agtggttttt gcaagaacag
     7621 caatcgttta tggtatctaa tccgtcatgg attgttgaag gaaattatac ttctacatta
     7681 gaggaaagga ttaaagaagc agataagatc atttggctta aagtgccgcg ttatctcgcc
     7741 atctaccgag tagtttggcg ttctctaaaa agaaagataa acaaaaaatc tcggccagat
     7801 atgcctgaat cattttcaga gaaactaaac agggaatact tagaattttt atcttttatc
     7861 tggcattttg aagaaaacaa tgaaccgaaa atacagcagc tgatccaagt agctaatgca
     7921 agaaataaac tgatcatcct taaaacaaga cgagagaaac aagaattttt aactaaattg
     7981 aaaatagaga gcaaaaataa ttgacagttt ttgtttacaa gcgtaatctg tattcataag
     8041 ctaatgatta catttgtaaa cgaaaggtgt gaaataaatg gaaaaagtca aaacgccagt
     8101 aaagatcagt gattcagagt gggagatcat gcgtgttatt tggacacttg gacagacgac
     8161 agcccaagaa atcacgcaga tcttagctga ttcgatggat tggaaaccag caacgatcaa
     8221 aactttgctg ggacgtttgg taaaaaaaga agtgatctgg acagaacaag aaggaaaaaa
     8281 attcatttat catccagctg tctcggagat ggaaacagtt cgcagtgcga cggaaaatct
     8341 tttttcccat atctgtgcaa aaaggattgg agagacgatt gcggatctag tagaagaagc
     8401 cactttgact caagaagata tcaatgagat tttgaaacaa ctggaacaaa agcaaccagt
     8461 agatacgatc gaatgtaact gtatcccagg tcaatgtgaa tgtaaacaac atcaatagga
     8521 ggaagaaaaa atgaaacaac aattttcaat cgaaggtatg agctgcaatc attgtgcagc
     8581 aagagtagaa gaaacagtaa gcgcacttga cggcgtgcaa aaagtgaaag taaatttgaa
     8641 aaaagcaaac ggtacggtca agtttgatga aacaaaagtt caatccgaaa agatttgtca
     8701 agcaatcaat ggattgggtt ataaagcaga ggtgatttga tggcaacagc agaaaccaaa
     8761 atggatacgt tcgtcattac aggtatgact tgtgcaaatt gttctgctag gatcgaaaaa
     8821 gagctgaacg gacaacctgg tgtggtcaat gccacagtca acttggccac tgaaaaagca
     8881 agtgtcaaat atgaagggac gaccactgaa aaattaatcc aaagcgtaga aaacatcggt
     8941 tatggtgcaa ttttgtacga tgaagcccac aaacaaaaaa ttgcagaaga aaagcaagcg
     9001 tatttgaaaa aaatgctatt cgatcttata ttgagtacgg ttttgacttt gccgttgatg
     9061 ttgtcaatga ttgctatgat gttaggaagc catgcggcga ttgttcactt ttttcatttt
     9121 ccaatcgtcc agttagtgct gtctgctcct gtccaatttt atgtcggtgc tcgtttttat
     9181 aaaggtgcgt accatgcaat caagacgaaa gcaccaaata tggatgtatt ggtagcaatt
     9241 ggaacatcgg ctgcctttgc attgagcatt tacaacggat ttttccgtgg acacccccaa
     9301 gatctttatt ttgaaagcag cagtatgatc atcacgctga ttttgctcgg caaatatttg
     9361 gaacatacag caaaaacgaa aacaggtaat gcaatcaaac aattgatgtc tcttcaaaca
     9421 aaaaccgccc aagtgataag aaacgggaaa gaagaaacgt tagcgattga agaagtagtc
     9481 gttggtgatc aactagtcat ccgtcctggt gagcagattc cagcagatgg tcgaattatt
     9541 tcaggaagca gtgcgataga tgaaagtatg cttactggtg aaaatcttcc tgtagaaaag
     9601 aatccggatg acacgctttt tggcgggaca atcaatacga atggtctgct acacatggaa
     9661 gtcacacaag tcggcaagca gacagtatta gcgcaaatca ttcaaatggt ggaagatgct
     9721 caagggagca aagccccaat ccaaaaaatc gcagatcgga tctcagggat ttttgttccg
     9781 atcgtattag tcatcgcgtt tatcacgctc atagctacag gtttgatcac tggtgattgg
     9841 caactcgcat tgatccatag tgtctctgta ttagtcattg cgtgtccatg tgcattaggt
     9901 ttagcgaccc caacagctat tatggtagga acgggtgttg gtgcacgaaa tggaatttta
     9961 attaaaggcg gagaagcatt agaagcagct gctcacttag acagcattgt tttggataaa
    10021 actggaacga ttacagaagg aaagcccaaa gttacagatc tagtcggttc aaaagaagtc
    10081 ctatctattt tttatacttt agaacaagct tctgaacatc cactaggtaa agcaatcgtg
    10141 gaatacggaa aactgcaaga agcagcaaca tacgatatga ttgattttac agcccaccca
    10201 ggtgctggga tcagcggtac gattaatgga gtacgctatt ttgcgggtac acgtaaacga
    10261 ttaattgaat tgaacctatc atttgatgaa taccaagaac acgcgttaga attagaacag
    10321 cagggaaaaa cagtcatgtt tttagctgat gaaaagcaag tgatcgggtg gattgccgtg
    10381 gctgatcaaa tcaaactaga agccaaacaa gcaatcaagc agctccagaa taaaggatta
    10441 gatgttttta tgctgacagg agacaacaaa ctggcagccg aaacgattgg taaacaagtt
    10501 ggtattgatc caaagcatat ctttgctgaa gtacttccag aggataaagc agcctacgta
    10561 gaaaaacttc agaaagatgg caaaaaagtc ggtatggcag gcgatggaat caatgatgca
    10621 ccggccttag cattagctga tgtcggtatg gcaatgggaa gtggaacgga cattgcgatg
    10681 gaaacagccg atgtgacgct gatgaatagc agtttggcat ccattgcaca aacaatcgag
    10741 ttgtctaggt tgacattgag aaaaatcaaa caaaatcttt tctgggcttt tgtctataat
    10801 acgattggta ttccttttgc cgcactaggc tttttgaatc caatcattgc aggaggagcg
    10861 atggcgttta gttccgtcag tgtattgttg aactccctga gcttaaatcg acacatgaaa
    10921 aagtaagaga agggttggag taaaaatgaa tgggaacgat atggagaaaa aacatgagca
    10981 taaaaaagaa gtagtaaaca aagaaagtac agaatctcat gaaaaacaga tggagatgaa
    11041 acatgaccat agtcagatgg atcattccat gcacatggga cataaccatg gagatatagc
    11101 aaacagcaaa caaatggacc attcgatgca catggatcac gaacacggcg gcatggatca
    11161 ttccatgcac atggggaatt tcaaacaaaa attttggcta tcgcttatcc tagctatccc
    11221 aatcattctt ttttcaccaa tgatgggaat ggaatttcca tttcaagtaa cgtttcctgg
    11281 ttctgattgg ctcgtattga tcttagcaac tatactattt atctatggcg gtcagccatt
    11341 tttaagtgga gctaagatgg aattgaaaca aaaaagtcct gctatgatga cactgatcgc
    11401 gatggggatt acagttgcat atatttatag tgtctattcg tttatcgcaa atctgctcaa
    11461 tccacataca cacgtcatgg atttcttctg ggaattggct actttgatcg ttattatgct
    11521 actaggtcac tggattgaaa tgaatgctgt gtcgaatgcc agcaatgctt tacaaaaact
    11581 agcagaatta ctgccagaat cagtgaaacg cttgacgaaa gatggaaaag aagagacagt
    11641 ttcattgaaa gaagtaaacg aaggtgatcg tttgatcgtc cggtctggtg ataaaatgcc
    11701 aacagatggt gtaatcttga aaggtgaaac aattgttgac gaatcagctg tgactggcga
    11761 atcaagagga atcaaaaaac aagccaatga caaagtgatt ggcggttcta tcaatgggga
    11821 tggaacgatc gaaatcgaag taactggaac tggtgaaaat ggttatctag cgaaggttat
    11881 ggaaatggtg cgaaaagcac aaggagaaaa atccaagcta gagtcattat ctgacaaagt
    11941 ggcaaaatgg ctgttttatg tcgcattaat tgtgggtatc ctggcattca tcgcctggtt
    12001 gttcttgaca gatcttccta atgcattaga acgtatggta acagtcttta tcatcgcctg
    12061 tcctcatgct ctaggtttag ctatcccatt agttgttgct cgctccacat caatagcagc
    12121 taaaaacgga ttacttttga aaaaccgcaa tgcactggag caagccaatg atctagatgt
    12181 gatcatgctg gacaaaacag gaacactaac agaaggaaaa tttactgtaa cgggtgtcga
    12241 agtacttgat gatgcgttca ataaaaatga aatccttcaa tatctaggag ccttggaagc
    12301 gaatgccaat catccattgg ctgttggcat tatgaattat ttgaaagaac atgaaatcaa
    12361 gccttatcaa gctgaaaatc taaaaaatct ttcaggagtc gggctagagg caacagtcaa
    12421 aaaacaacaa gtgaaaatag ttaatgaaaa agaagttgag cgtctcggat taaacgtaga
    12481 acaagcatta ttgaaacctt accaagaaca aggaaatacc atcagcttcc tgattttaga
    12541 aaatcatcta gctgcaatcg ttgctttagg tgacgtagta aaaacagaag caaaagaatt
    12601 tatccgaaca ttgaaagaaa gaaaaatcac tcctgtcatg cttactggtg acaataaaaa
    12661 cgctgcccaa gcagtagcag attatttagg gatcgaagaa tattatggcg gtttattacc
    12721 agatgataaa gaagctgttg tccagaaata ccttgaccaa gggaaaaaag taatcatggt
    12781 gggtgatggg atcaatgatg cgccaagttt agcccgtgct tcaatcggga tggcaatcgg
    12841 tgcagggaca gatatcgcaa tcgattctgc ggatgtcgta ttgacaaaca gcgatccaaa
    12901 agatattttg cattttcttg atctagccaa gcaaacgcga aagaaaatga tccagaacct
    12961 ctggtggggg gcaggttaca atattcttgc aattccgcta gcagccggaa ttcttgcgcc
    13021 aattggtatc atattaagtc cagcagttgg tgctgtcttg atgtcgttaa gtacggtagt
    13081 tgtagctcta aatgctttga cattgaaaat caaataataa tgaaaaaa
//
LOCUS       DACEXQ010000055        13097 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.055, whole genome shotgun sequence.
ACCESSION   DACEXQ010000055 DACEXQ010000000
VERSION     DACEXQ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.055"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..178
                     /locus_tag="IXH04_001150"
     CDS             <1..178
                     /locus_tag="IXH04_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1851770.1"
                     /translation="SKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYN
                     ESTETSHSEENLSW"
     gene            369..1556
                     /locus_tag="IXH04_001151"
     CDS             369..1556
                     /locus_tag="IXH04_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ1851771.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYFYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVVGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            1562..1918
                     /locus_tag="IXH04_001152"
     CDS             1562..1918
                     /locus_tag="IXH04_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAQ1851772.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            1920..2258
                     /locus_tag="IXH04_001153"
     CDS             1920..2258
                     /locus_tag="IXH04_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAQ1851773.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            2653..3687
                     /locus_tag="IXH04_001154"
     CDS             2653..3687
                     /locus_tag="IXH04_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1851774.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            3680..4366
                     /locus_tag="IXH04_001155"
     CDS             3680..4366
                     /locus_tag="IXH04_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1851775.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            4390..5238
                     /locus_tag="IXH04_001156"
     CDS             4390..5238
                     /locus_tag="IXH04_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1851776.1"
                     /translation="MKKKIFGLAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDNIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            5435..6208
                     /gene="sufC"
                     /locus_tag="IXH04_001157"
     CDS             5435..6208
                     /gene="sufC"
                     /locus_tag="IXH04_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAQ1851777.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFNGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            6221..7507
                     /gene="sufD"
                     /locus_tag="IXH04_001158"
     CDS             6221..7507
                     /gene="sufD"
                     /locus_tag="IXH04_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAQ1851778.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSTVDQLGKNITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            7504..8739
                     /locus_tag="IXH04_001159"
     CDS             7504..8739
                     /locus_tag="IXH04_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ1851779.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAH
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     SFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVINPVQELAELVHENGGVIVVDGAQSAPHRAIDVQALDCDF
                     FAFSGHKMCGPTGIGALYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQELGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            8726..9196
                     /locus_tag="IXH04_001160"
     CDS             8726..9196
                     /locus_tag="IXH04_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAQ1851780.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            9201..10592
                     /gene="sufB"
                     /locus_tag="IXH04_001161"
     CDS             9201..10592
                     /gene="sufB"
                     /locus_tag="IXH04_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAQ1851781.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(10687..11829)
                     /locus_tag="IXH04_001162"
     CDS             complement(10687..11829)
                     /locus_tag="IXH04_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1851782.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(11868..12602)
                     /locus_tag="IXH04_001163"
     CDS             complement(11868..12602)
                     /locus_tag="IXH04_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1851783.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            12834..>13097
                     /locus_tag="IXH04_001164"
     CDS             12834..>13097
                     /locus_tag="IXH04_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1851784.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMER"
BASE COUNT         4170 a         2444 c         2797 g         3686 t
ORIGIN      
        1 gtctaagcgc tctgttccag aaaatgtgcc aatgactccg ttgaatttcc atcgttattt
       61 ttatttcatc caacgaacca atctggtttc tgatcagctg ctagagattg ccgaagagat
      121 ccttcgatac aatgaaagca cagaaacttc tcacagcgag gagaatctca gttggtagat
      181 aattgcatgt atcaaaaaaa gttctatgat acactaaaga taagcagtag gcttttgtac
      241 tgcatttgct caagcaactg cacaaacgga aagttaattt ttcccaaatg tctatggaaa
      301 gactttccat tatctaactg gaccgctata ataatcagca gtatggtttt taagaaaagg
      361 gatgaaacat ggaaaaaaaa ttaaaagctt cgaacgataa tcgtattgat tatggtgtga
      421 ttttaccagt ctttctgtta tgtttgattg gtctgctctc attatatgta gcacttagcc
      481 atgatccgaa caatccagca gtcttgaaag gcgtagggat gcaagttgtc tggtatttag
      541 ttggagcggc agcaatcgtt gtcatcatgc atttgaattc caaatggatc tggaaactga
      601 ctccttactt ttatggtttg gggttagtgg taatgggact cttgctcaaa ttctatgatc
      661 agaatttagc tgcttcaaca gggtctaaaa actggtttcg attcggtgcg tttactttcc
      721 agccagcaga gttgatgaag attgcgtaca tcttgatgat ggcactgatc gtaactaaac
      781 ataatacgca agtaaaagaa cggacgatga aatcggactt ttggctgatt gggaagctct
      841 tgatcgtgac aataccggtt ttagcattga tcatggccca agatgacttt ggaacgatgc
      901 ttgtctttct tgcgatcttt ggcgggattt tcttgatgtc tggaatctct tggcgcatca
      961 tcgcacctgt agtaatcttg gcagtagtcg ttggagcagg aacgatcttc ttggttacga
     1021 ctgaaggcgg aagagacttg ctttacaaag ttggcttcaa atcttatcag tttgcacgta
     1081 ttgattcatg gctggaccct ttccatgata cgtcaggaat gagttatcag ccggcccaag
     1141 gacttttggc aattggtacc ggtggcttgt ttggtaaagg attcaacgta agcaatattt
     1201 atgtgcctgt tcgtgagtcc gatatgattt ttaccgttgt cggtgaaaac ttcggcttta
     1261 tcggtggtgc atttgtcatt ttcttgtatt ttattttaat ctatcgtatg attcgggtgt
     1321 gtttcgatac gaataatgaa ttttacgcct atatcgcaag cggtttgatc atgatgctgc
     1381 tgttccatgt ctttgagaat attggagcaa atatcggatt acttccttta acagggatcc
     1441 cattgccttt cattagtcag ggaggatctt cgattttagg gaacatgatc ggtatcgggt
     1501 tgatattatc gatgagatac caaaatgaag cacctgcaag atcttcaagg aggtaagaaa
     1561 attgtacacc ttttactggt acccgaaatg ttcaacatgc aaaaaagcca aagcgtggtt
     1621 agaccagcat ggcgttagtt atcaaactgt tgatatgatc aaagaagtac cgaaggcctc
     1681 aacgattgag acttggctag aaacgagtca agtaccaatc cgtcgcctgt tcaacacaag
     1741 cgggatgaaa tatcgggaac ttggactgaa agatcaagta ggcgagttta cggcttcaaa
     1801 agcgagccaa gtcttatcga cagatggtat gctgatcaag cggccactga tcttacgaga
     1861 ggaccagctt gttgcaattg gattcaatga aaaaatttat gaaggagaac tgatataaaa
     1921 tggcaggcaa gcatttgaag aaaaaggaca acctttggat cttatttaat ggaaaagaat
     1981 actgtgttgg tttgacaaat gaagcgcaag aagaacttgg agacatcacg tttgccaatc
     2041 tgccaaaagc aggacaatcg ctaaaaaaag atgaacctct gatcgaagtt gaagcggaaa
     2101 aagcggtcaa tgaatttgcc agcccgctaa cgggtacagt ttcttctgtt aatgataaaa
     2161 tcggtgaaga tatcaatatc ttgaatgatt cagatgaaat gaatgcgtgg atcttaagtt
     2221 tcaaagatgt tgatccaaaa gaatttgacg aattataaaa tcagaaaatc acttgaaaaa
     2281 agttgacact gattcttcca gttgctataa tcataagcaa tcctatatga aacgagaaag
     2341 ctttgaaaag aagagtacat gtcacggacg tttgaagaga gccctgtttg ctgaaaatgg
     2401 gtaacgaaaa tgagatggaa gatggtctta gagcagagta agcgagcgta agcaaactta
     2461 ctacgggagt gcccgttaca gcgcgacagt atacggaaag aagctaagtc caagtactgg
     2521 aagaggctgt tattgcgaag taacggtaaa tcaaggtggt aacacggaag ttcaagcttt
     2581 cgtccttttg cttaaggcaa gagggcgaag gcttttttgt ttgaaaaatc ataagagaaa
     2641 gatgtgaatg gcatgtcatt aatccaacta acgaatgtca aaaagacatt caatgggaaa
     2701 aacggaaaca tccaagccgt caatgacgtt tcactaaacg ttgaaaaagg ggatatttat
     2761 ggaatcgtcg gctattctgg cgcaggaaaa agtacattag tccgtctcct gaatggactt
     2821 gagcttccca cgagcggcga agtgatcgta aacgatcaag atattacaaa attaaaaaac
     2881 aaagaattac gtacattccg aaagaaaatc ggtatgatct tccagcattt caacctgtta
     2941 tggtcaagaa ctgtacttga aaatatccag ctgccgctgg aattagctgg cgtaccaaag
     3001 agcaaacgta aagaacgggc agaagaactg ttgcgtttag tcgggttaga aggacgcgga
     3061 caagcatatc cttcacaact ttccggcggg cagaaacaac gcgtcggtat cgcacgagca
     3121 ttggcaaatg atcctgagat cctgctttgt gatgaggcta ctagcgcatt ggacccgcaa
     3181 acaacagaag aagtattaga cttattgtta gcgatcaata agaaattgaa tttgaccatc
     3241 gtcttgatca cacatgaaat gaatgtgatc cgcaaaatct gcaacaaagt cgctgtgatg
     3301 gagttgggga aagtcgtaga agaaggagac gtattgactg tcttcagaca accgaaaaaa
     3361 gaagtaacca aacgctttgt acaacaagac atcgaaccag aagctgattc gacagaacta
     3421 ttagctgaac tcatcaaaga aaatccaagc ggccgtttgg caactttgac ctttagcgaa
     3481 agaaatgcca acgagccagt catttcccaa gcgattcgac gctataacat cgatgtcaac
     3541 gttgtgtatg gaaagatcaa acaagtgaaa gaaggttcct ttggttcatt gactgtcttg
     3601 atgactggtg aaaccaaaga attagatcaa gcagaagtgt tcatcaaaga acaaggagta
     3661 ggaattgagg tgatccatcg tggataaaag cttttcagaa acatatctag attttaaaca
     3721 aatcaacccg gaaacgatga atcaggctat ccttgataca ctatatatga cattaatctc
     3781 aatggtctta gtgacgatca tcggattagt tttgggattg atcctttact ctattggacg
     3841 caaaaacaaa ccgtatgctc gaatcattta tggaatcgta tcgatcatca gtaatatttt
     3901 ccggtcaacg ccatttatga tcctgatggt tttgatcatt ccatttacta aagcaatcgt
     3961 aggcactatg ctaggtgcgc aagcagccat tccagctctt gttttatccg cagcaccatt
     4021 ttatgcccgt ctagtagaga ttgcttttcg tgaagtcagc cctggtgtac tggaagcagc
     4081 agatgcaatg ggagcaagtt actgggaaat catctggaag gtcttgatac ctgaaagtat
     4141 tcccgcattg atttctggat taacagttac aacgatctcg atgatcggat ttactgcgat
     4201 ggccggcgcg atcggcgcag gtggacttgg aggtcttgct tggcaggaag gctaccagcg
     4261 tggtaatctg acagtgacat tcgttgcgac tttaattatt ttactaatcg tatttatcgt
     4321 tcaaggaatc ggtgatttct taactaagaa aacagacaaa agataatagg atataggggg
     4381 atgtaggata tgaaaaagaa aatttttgga ttagcagcag cgcttttgct gactgtagga
     4441 ttagcagcat gcggtaacaa cagcgacagc aaagattcag caaataaagc agacgacacg
     4501 acattgaaag tcggcgcttc accgacaccg cacgcagaga ttttggaaca tgtgaaacca
     4561 ttattgaaag atgaaggaat cgatcttgaa atcgtcaaat tcgatgacta tgtattaccg
     4621 aaccaatctt tggaagaagg cgacattgat gccaattact tccagcatat tccttatttg
     4681 aacaaagaaa tcaaagaaaa aggatatgac tttgtcaatg ccggtgctgt ccacatcgaa
     4741 ccaatgggct tgtactctaa aaaagtcaaa gatgtttccg aattaaaaga tggtgcaaca
     4801 atcattactt caaattctga atcagactgg ggacgcatca tcacaattct acaagatgct
     4861 ggcttagtca aagtcaaaga tggagtagat ctggaaacag caacattcga taatattgaa
     4921 gaaaacccta agaacttgaa gttcaaccat acgatcgacc catcactttt agcttctacg
     4981 taccaaaatg atgaaggtga cctagttgca atcaacgcaa acttcgctta cggcgcaggg
     5041 ttgaatccat tagaagatgc cgtattgctt gaaaaggaca actctcctta cgtgaatatt
     5101 attgcgactc gtaaaggtga tgaagacagt gacaaaatca aaaaactgat tgaagtgctt
     5161 caccgcaaag aagtccaaga atggatcaca gacaaatggg gcggctctgt taaaccagta
     5221 aaggctgatg caaaataagg tatggaacaa aaaagatgag aaataagtct gtaatggctt
     5281 atttctcatc ttttttatat ctattctaat ttagaaatca aaattgttta ataataaata
     5341 aaagatattt gattttagta aggaaggtca ttgcgcttca ttctcaatta gtctaaaatg
     5401 agtgagaacg aaagagaatt tgggggtata atgcatgtct gtcttagaaa taaaaaatct
     5461 acatgtgtcg atcgaggaaa aagaaatcct taaaggcgta aacctaacaa tgaaaaccgg
     5521 agaaatccat gccatcatgg gacctaacgg cacaggaaaa tctaccttat ctgccgctat
     5581 catgggcaat ccaaactatg aagtaacaga aggcgaaatt ttgtttaacg ggaaaaatgt
     5641 tttagacttg gaagtagatg aacgtgcgcg acttggctta tttttagcta tgcaatatcc
     5701 aagtgagata ccaggtatca cgaatgcgga attcatgcgt gcagcgatca atgcgaaacg
     5761 tgatgaaaac gacaaaattt ctgttatggc atttttgaaa aagttagatg aaaaaatggc
     5821 tttactaaat atgccagaag aaatggctga acgttatctg aacgaaggtt tttctggcgg
     5881 agaaaagaaa cggaatgaga tcctgcaatt gttgatgtta gaaccaacat ttgcaatttt
     5941 agacgaaatc gattcaggtc tggatatcga tgctttgaaa gtcgttgcta aaggagtaaa
     6001 tgaaatgcgc ggagacaatt tcggtgcatt gatcatcacc cactatcaac gattattgaa
     6061 ctatatcaca cctgatgtcg ttcacatcat gatggaaggc cgagtagtca tgacagggaa
     6121 tgctgaccta gctaaacgtc tggaagcaga aggctacgct ggtatcagta aagaattagg
     6181 aatcgactat aaagaagaag aagcgtaagg gggagcaaga atgaaagaaa aaaactggct
     6241 cgattatctt gacgcggtaa atgatttttc tttaagtaaa ggagaacctg attggatgag
     6301 aaccttccgt caagacgcac tagcgaaagc agatgaactg ccattgccgc atatcgatcg
     6361 ggtgaaattc catcgctggt cgttatttga tgtgaaagaa acacaaacca tcagtgaaac
     6421 aggcacgatt ccggcatttg atgcaatgaa agacaatcct gtgcttgtcc aacaaggttc
     6481 atggacgatt tttgaacaac tgcctgtgga attagcagaa aaaggagtca tttttaccga
     6541 cttgttcact gcaatgatcg aatatccaga actagtccaa gaatattata tgaaaaaagc
     6601 agtaaacatg aacgaagacc aattgactgc actccatgta gcttttatga acagcggtat
     6661 tttcctgtat gtgcctaaaa atgtcgtgat cgatgaacca ctcgagtcct tattcattca
     6721 agatggcgca tccgatgagc acttcttcaa gcatgtgttg atcgtggcag atgaacatag
     6781 tgaattttct tatctggaac gtttccagac aaccaaagaa caagtagcaa aatcatcagg
     6841 taatatcatc gtggaagtca tcgctaaagc tggttcaaaa atcaaatatt caaccgtaga
     6901 tcaattagga aaaaatatta cttcttatat gaatcgccgt gggcacattt tacgagatgc
     6961 ttctgtcgac tgggcaattg gtgtcatgaa tgacggtcat gtgatcgctg actttgactc
     7021 agatttagca ggagaaggtg cacacgcaga agtcaaaatc gtggcaatca gctcgggcag
     7081 acaaatccaa ggaatcgata cgcgagtaac caataaagcc cctcatacga tcgggcatat
     7141 tttgcaacac ggagtgatcc gagaaaaagg aacattgacc tttaatggta tcggccacat
     7201 cttgaaaggg gcaaaaggtg cagatgccca acaagaaagt cgtgtcttga tgctttctga
     7261 taaagcacgt ggtgatgcga atcctattct tttgatcgat gaaaacgaag taactgctgg
     7321 acatgctgcc agtgtaggac gagttgatcc agaagaaatg tactatctga tgagtcgagg
     7381 attgcataaa gaagaagcag agcgcttagt gattcgagga ttcctcggtt ctgtcttaac
     7441 agcaatccct gtagaacaag ttcggaaaga attagtcgaa gtaatcgaag ggaagttgaa
     7501 tggatgatcg atgctaaaaa gctacgcaat gattttccta tcttatttca agacgtcaat
     7561 gatgaacctt tagtttatct ggataatgca gcgacgaccc aaaagccaaa acaagtactc
     7621 caggcattag cgcactatta cgaacacgat aacgccaatg ttcatcgcgg tgtccatacg
     7681 ttagcagaac gcgcgaccca tgattatgaa caagcaagag aaaaagtacg ggcgttcatc
     7741 catgcaaaag aaacagcaga agtgctgttt actcgaggta cgacgacaag cttgaattgg
     7801 gtagccagaa gcttcggcga ccgctatatc aaagcaggag acgagatagt gctctcttac
     7861 atggaacatc attctaatgt tatcccgtgg cagcagttgg caaaacgaac aggtgctgtc
     7921 ttgaaatata tcgaactaac agaagaaggc ttcctggata tggaggatgc agcgaaaaag
     7981 attacggaca aaaccaaaat cgtctccatt gctcatgtat ccaatgtctt aggtgtcatc
     8041 aatcctgttc aagaattagc tgaacttgtc catgaaaacg gcggggtcat cgtagtagat
     8101 ggtgctcaat ctgccccgca cagggccatt gatgtccaag cactagactg tgatttcttt
     8161 gctttcagcg gacacaaaat gtgcggacca acaggtattg gtgctttata tggtaaacga
     8221 aaatggttag aagaaatgga acctgttgaa tttggtggcg aaatgatcga ttttgtcgaa
     8281 ttatacgaca gtacatggaa agaactacct tggaaattcg aagcagggac accaaatatc
     8341 gccggcgcta tcgcattagg ccatgcaatc gattatttgc aagaactcgg catggacaat
     8401 atccatcgat atgaagaaga actagccgct tatgtgttgc caaaattaca agcaattgat
     8461 ggattgactg tttatggtcc acaagacccg aattttcgga caggagtcgt tgcattcaat
     8521 ttggatgggt tacatcccca tgatgtcgcg acagctttag acatggaagg ggtagccgtc
     8581 agagcaggtc atcattgtgc gcaaccgcta ttgaagtacc ttgaagtatc agcgactgct
     8641 cgcgcgagct tttatttcta caacacaacg caagacgccg accgtttagt tgaagcaatc
     8701 atggcaacaa aggagttttt ccagcatggg actatctaaa ttagacaatt tataccgtca
     8761 agtgatcttg gatcattctt cccatccgca tcatcgtgga agcttgactg actcgacaag
     8821 taagatcgaa ttgaacaacc ccacatgtgg agatgtgatc gaattgcaag tcgatgtaaa
     8881 agacggaatc atcaaaaata tcgcctttga cggcagtggc tgctccatca gtacggcaag
     8941 tgcctcgatg atgacagacg ccgtcatcgg aaaatcaata gaagaagcag aacatttaac
     9001 tgtagacttt ctacagcttg tccaaggaaa agaagtcaaa gatcccgacc aattaggcga
     9061 tgctgctatg cttggtggtg tagctaaatt tccagctcgg atcaaatgtg cgacacttgc
     9121 ctggaaagca ctaggaaaag caatggaaaa cgatggtacc gcagctttcc atcattacca
     9181 caaggaagag gagtgagaac atgggcgtac cagaattaga agaatataaa ttcgggtttc
     9241 acgatgatgt cgaaccggtt ttcagtacag gagaaggctt gacagaagaa gtgatccgtg
     9301 agatgtcaag aatcaaaggc gaaccagaat ggatgcttga attccgtctg aaatctctag
     9361 aaacatttaa caaaatgcca atgcaaacat ggggccccga cctttcagac atcgatttcg
     9421 atgccatcaa atattaccaa aaaccaagtg acaaacccgc tcgtgactgg gaagacgtac
     9481 cagaaaaaat caaagataca tttgaaaaga ttggtatccc agaagcagaa cgagcttatc
     9541 tagccggtgc ttccgcacaa tatgaatcag aagtcgtgta ccataatatg aaagatgaat
     9601 tcgaaaagtt aggaatcgtc tttactgaca ctgactctgc tttgaaagaa taccctgaat
     9661 tattcaaaga gtacttttca aaacttgtac cgccaactga caacaaatta gcagcgctga
     9721 attcagccgt atggtcaggt ggcaccttta tttacgtgcc aaaaggcgtc aaagtcgatg
     9781 ttccattaca aacttacttc cggatcaatg cggaaaacac gggacaattc gaacgtacgc
     9841 tgatcatcgt tgacgaagga gcaagcatcc actatgtcga aggttgtacg gcacctactt
     9901 attcaagtaa tagtctgcat gccgccatcg tagaaatctt tacaagaaaa gatgcgtatt
     9961 gccgttacac aacgatccaa aactggtcag acaatgtgta caacttagta accaaacgag
    10021 caaaagcata cgaaggcgca accgttgaat ggattgacgg aaacctaggt gcaaaaacaa
    10081 cgatgaaata cccaagtgtt tacttagatg gaaaaggcgc acgaggcacc atgctctcca
    10141 tcgcctttgc aggagcaaac caaatccaag atacgggtgc aaaaatgatc cacaatgcac
    10201 caaatacatc tagctctatc gtctctaaat caatctcaaa agacggcgga gaagtaaact
    10261 atcgaggaca agtaaccttc gccaaaaaca gtgcgggttc gatctctcat atcgaatgtg
    10321 acacaatcat catggacgaa tggtcgaaat ccgatacgat cccatttaat gagatccaca
    10381 acagccaagt ctctttagaa cacgaagcca aagtctcaaa aatctcagaa gaacaactct
    10441 actatctgat gagccgaggc ctgaccgaat ctgaagcaac tgaaatgatc gtcatgggct
    10501 ttgttgaacc attcaccaaa gaacttccaa tggaatatgc agtcgaactc aaccgattga
    10561 tcagttatga gatggaaggc agtgttgggt aaacctgtta taggagctag aacccttgat
    10621 atataagggt tctagctttt ttgttttgcc tgtatactag aaatgaccag attttgacca
    10681 gctcttttac tgatgagcca tcatatcagc aaagtcttga cctgttttct taggtgcatt
    10741 atttgtaata tgtgcataga tgtctaatgt cactttacta ttttcatgcc ctaatcgttg
    10801 ttgaactacc ttgacagatt ctcctgcttc taataataaa ctagcatgtg tatgtctaaa
    10861 cccatgaggt gtaatacgag gcaagttata ttttttcaaa atatatctta accaatcatt
    10921 aacagtgttt ggaatgtata gagtgttttt aactgttgta aaaacatgtt gctctttaga
    10981 gctagtattg tatccaagtt ttaaatactc ttctttttgc tgtaatttcc agttgttgag
    11041 gatagaaaga gtctcagtat ctaaagagat aactcgtcta gaagctcttg ttttcggaga
    11101 ttgaattata actttaccaa attcatctac agcaagagtt ttattgacat gtagttcttt
    11161 attaaacgtg tctatgtctt tccactgtaa agcgagtact tcactctttc tcatacctgt
    11221 gtatgccaat aatctgaaga aggcgtattg tttcatgtta ccaaaatctt taaaagcatc
    11281 cagtaaatta ttaagctcct cttttgagta aaatttacga tttttctcat actctttttt
    11341 tcgtggaaga agtgtttttt ccataggatt attgtcaata tattgcattg caaccccgta
    11401 gcgtaaaatt tgtgcagtct gttttctcat ataatcgtaa ctttcatatt tactgtgcca
    11461 ttcatctacc actttttgac aatatgggac agagatattc gatagtttaa gcttaccaaa
    11521 tgctggaagt atttgattat ttgcgtatct tactgagatt gcgattgttg aagctttaac
    11581 actggtacgc tgatgagtta tccattcatt atacaggtct tcaaatgtca tatcaggtgc
    11641 tttcttttta acaccgtata atatttgatg tctgtactcg tcataatctt tttgagcttc
    11701 ttgtttgcta ctaaaacctc ttctcgtagt atttattttt tcaccagttc gtggatcggt
    11761 ggcaatgtgt cctttataca tccacgccac ttgaccgttt ttctttttgt actttgatat
    11821 ttttgccatt aaatcatctc ctcttagttt acaaggagta cttaccacta gatggtatca
    11881 cctcccttca tttttttctt agtgtatttt ttcccaattg atgaaatcgt ttgttctagc
    11941 ttgtttatat tatcagtaat ttctctcaca gcttcttcgg gataagacat gtctttcaat
    12001 tgaataggta gccattcatc taccattctt tctagtagtt cttctatact gctagtgagg
    12061 aaaagaatgt tattgtcaaa ttcttctaca atagcattga tgaataattc ttctattttt
    12121 aagatattga aaagactaat tttttcattt ttaactttat tacaagtctt gttagcaata
    12181 aattcttgca tctctggttc taatttggaa tatctatcca gccatgtatc taaattagag
    12241 tgtactggtg ggaactgttg acgaagatgc tggaacaggt ttttatctgg aaatgttata
    12301 ccatctagta caatgctatc tctataaaga ataattccca aaatatattc ttctacattg
    12361 ccacaaacta actcctctat actcatattt cccaaatcag caattcggga taatcgctct
    12421 ggtattggta aataggttcc cttctcccat tcgtagatag tttttttgtc aattaattga
    12481 ccgttgttaa tcataagacc aaatgctggt tgtgttaatt ttttactctt tcttatagct
    12541 tttatttttg ttccgagtat cttttttcgt tctaaataat ttattggtgg acgttttata
    12601 atattgacca cacctcctaa aattagtata cctcaaaaaa ggggtgttgt aaataaattt
    12661 tacaaagctg ttgacacccc ttttttatcg aggtattatg tgtatgtcga tgaactggta
    12721 atcatttttt gtttgattaa tcggttaata acaaaatact tttattttga gcaaaaaata
    12781 cgaagataat tcttagaaaa tttgttcaag caatcacgaa aggaggggga aatatgtctc
    12841 tacaaatcat tgatagcatt gtttctatac aagttgaaca acaagtgaaa gagataatta
    12901 cagtgctaaa gcctatgcta gaagacatta tttctggagt cactctagag tatatggact
    12961 atcagcaggc aagtgaatat ctaggtgtat cagtaggaac gattagaaaa tatgtttctc
    13021 aatacgggtt acctgttata aaaattgata cggtcattag atttaaaaaa tctgatattg
    13081 atagtttcat ggaacgg
//
LOCUS       DACEXQ010000056        12960 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.056, whole genome shotgun sequence.
ACCESSION   DACEXQ010000056 DACEXQ010000000
VERSION     DACEXQ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12960
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.056"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            581..1270
                     /locus_tag="IXH04_001165"
     CDS             581..1270
                     /locus_tag="IXH04_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1851785.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1257..2465
                     /locus_tag="IXH04_001166"
     CDS             1257..2465
                     /locus_tag="IXH04_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1851786.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2759..4030
                     /gene="serS"
                     /locus_tag="IXH04_001167"
     CDS             2759..4030
                     /gene="serS"
                     /locus_tag="IXH04_001167"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAQ1851787.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4579..6063)
                     /gene="guaB"
                     /locus_tag="IXH04_001168"
     CDS             complement(4579..6063)
                     /gene="guaB"
                     /locus_tag="IXH04_001168"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAQ1851788.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6568..7263)
                     /locus_tag="IXH04_001169"
     CDS             complement(6568..7263)
                     /locus_tag="IXH04_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAQ1851789.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7353..8453)
                     /gene="ychF"
                     /locus_tag="IXH04_001170"
     CDS             complement(7353..8453)
                     /gene="ychF"
                     /locus_tag="IXH04_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAQ1851790.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8462..8653)
                     /locus_tag="IXH04_001171"
     CDS             complement(8462..8653)
                     /locus_tag="IXH04_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAQ1851791.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8674..9564)
                     /locus_tag="IXH04_001172"
     CDS             complement(8674..9564)
                     /locus_tag="IXH04_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAQ1851792.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9551..10318)
                     /locus_tag="IXH04_001173"
     CDS             complement(9551..10318)
                     /locus_tag="IXH04_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ1851793.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10330..11043)
                     /gene="rsmG"
                     /locus_tag="IXH04_001174"
     CDS             complement(10330..11043)
                     /gene="rsmG"
                     /locus_tag="IXH04_001174"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAQ1851794.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11275..>12960)
                     /gene="mnmG"
                     /locus_tag="IXH04_001175"
     CDS             complement(11275..>12960)
                     /gene="mnmG"
                     /locus_tag="IXH04_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAQ1851795.1"
                     /translation="GKNIDKTYIQMRMLNTGKGPAVRALRAQADKHAYSTEMKHTIEK
                     EPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTALRGEIIIGELKYSS
                     GPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTEEQPGDKEPNHFSFS
                     TPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVGARYCPSIEDKIVRF
                     ADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGLEKVEMMRTGYAIEY
                     DVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGINAALKVQGKEPLVI
                     KRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLRLTEMGHRVGLVKED
                     QYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKDGVLASDFLRRPEIT
                     YAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKRMEAKKIPENIDYAA
                     INGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQGKIAKVSEPA"
BASE COUNT         3638 a         2622 c         2412 g         4288 t
ORIGIN      
        1 ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac aagtaattgt
       61 caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc aactcattta
      121 tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc gatagaatca
      181 agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat ttccaacatg
      241 tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac aaataataat
      301 atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca tttctagctt
      361 acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc gagagacgta
      421 aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc aactagatgt
      481 ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg cttttttttg
      541 ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt tgattgctga
      601 cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg ggtatgaagc
      661 agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc ctgatattga
      721 attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag tgaaagagct
      781 acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg atatggataa
      841 gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta atccgttaga
      901 agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg ccccagatca
      961 gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg aagttaaaac
     1021 aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct acttgcttgc
     1081 tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt ggaaacaaga
     1141 aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag ataagattga
     1201 agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct ataaaattga
     1261 cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact tcaaaagaaa
     1321 tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta ttaaatgttt
     1381 ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg aatgcgattt
     1441 gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg aacggccgga
     1501 attttattat tccctttttc tttctattag atattggtgt tttgtattgg cgcttgattc
     1561 gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac atcgcaaacg
     1621 gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga gtggtaacaa
     1681 gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt aaaatcgaaa
     1741 aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca ctcacgtcca
     1801 tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa gatcttttaa
     1861 agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg gacgacttat
     1921 ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc tttgacatga
     1981 tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa aaaaatatcc
     2041 aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc gaaaaacttg
     2101 tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga gcaacgaaga
     2161 tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa aataacggag
     2221 caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt gtagaagaat
     2281 ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa agcattgtgg
     2341 ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct ttcatcatgc
     2401 accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt attgacgaaa
     2461 gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca cagaacccag
     2521 tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag gataatttgg
     2581 aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa cagccgttat
     2641 gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc agactgattc
     2701 gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag gaaatcatat
     2761 gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat tgatgactcg
     2821 gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc gtcgtaagct
     2881 actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg agatcgccca
     2941 attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag aagtcggtgg
     3001 caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga aacaaattgc
     3061 aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg aagaggacaa
     3121 tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa aaccacactg
     3181 ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag tagccggtag
     3241 ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct acaacttcat
     3301 gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt atatcgtcaa
     3361 cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg ttttccaatt
     3421 ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta actattataa
     3481 tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa gccctgcttt
     3541 tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc ttcaccaatt
     3601 caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag aattggaaaa
     3661 aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc gtgtgatggc
     3721 cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag aagtatggat
     3781 acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag atttccaagc
     3841 acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata ctcatactct
     3901 gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa actaccaaaa
     3961 tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga atctgaaagt
     4021 tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa aaattttgtg
     4081 aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact ggcttttgaa
     4141 caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga gaaaaaaatg
     4201 gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc agctgattga
     4261 acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca aggacaaatt
     4321 caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg tggcaaggga
     4381 acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa ataagaagaa
     4441 attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga aggagttgct
     4501 tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt tacttttgtc
     4561 ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt ttgtgatttg
     4621 tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa attgcgcttc
     4681 atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac gcaaaccgcc
     4741 gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac cttcgattcc
     4801 ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt cttttgaacc
     4861 tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac gtccttggaa
     4921 gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta gcatcactgc
     4981 atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc ctccgtctgc
     5041 aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag ctgtgagctg
     5101 aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc cgataccaac
     5161 ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg tcgcaacgtt
     5221 tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt ttcggatcac
     5281 acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg cttctaataa
     5341 agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta caagcaatcg
     5401 accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt ctttgatcgt
     5461 gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga ttttatgttt
     5521 ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca ctaagttgtc
     5581 ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca tgtcgcggtt
     5641 tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca caccactgat
     5701 acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg ttaagaagaa
     5761 cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat ccgcttgctg
     5821 gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca tagcaatggc
     5881 catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat tcaacttaat
     5941 atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac tttctgctgg
     6001 tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt cccagttgga
     6061 catttttagt accactcctc taatttagtt tatctattaa aataattgat tttttcattt
     6121 aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat ataaagcgtt
     6181 tacatctgtg tatattaaaa gagattgtaa aaaagctgtc ctgcatcaag taataagaac
     6241 agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac cgcagatgca
     6301 aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt cgagtagtaa
     6361 gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta ataccgagaa
     6421 actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt tgtcacagtc
     6481 ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc ctagatatct
     6541 agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac ttccaataat
     6601 cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga taatgataac
     6661 tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg cagaaaacag
     6721 tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca ttcctggttt
     6781 cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca tcatataaaa
     6841 gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag ctggcgttgt
     6901 tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa gcaacagcaa
     6961 cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg ctggcttatt
     7021 gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg ttcttccgcc
     7081 tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt tttcttcttc
     7141 agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt gatttctttt
     7201 ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt gttctggttc
     7261 cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca aaagacattc
     7321 taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa taacattacg
     7381 tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc ttcttttgct
     7441 gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc acggataaat
     7501 ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat tccttttttg
     7561 aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa gcctaacaaa
     7621 tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa agcttctaaa
     7681 aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg agcactgacc
     7741 acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac ataatgattc
     7801 gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat tggttttgtc
     7861 gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc gatcgttcgg
     7921 gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag ttctgccact
     7981 gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt gttgatagat
     8041 tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag tggatcaaca
     8101 cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg acaaatagcg
     8161 tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt acttgcgcct
     8221 tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt tttcggatgt
     8281 acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc tacgttgggg
     8341 tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat cgcattaaac
     8401 aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc cataaattta
     8461 ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca aaatctcgac
     8521 gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct gcgcccattc
     8581 gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca acgatatcgt
     8641 gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt gaatattcaa
     8701 gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga tcttaccttt
     8761 cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac gatcttcgct
     8821 ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct tttcttttcc
     8881 atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta aattttcagt
     8941 tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta acagcgtacg
     9001 ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat ccggtaaaga
     9061 aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac tagctacttc
     9121 tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt cttctaacgg
     9181 attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg cttcttcgtc
     9241 aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg ctcgaaaacg
     9301 tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga tgatgggttg
     9361 aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt cttcaaatgt
     9421 tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga caacggtatc
     9481 atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat cgattcctcg
     9541 gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct tggtagactt
     9601 cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta ggtgcttctg
     9661 ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag tatttacgta
     9721 cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc agtaagactc
     9781 cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta ttcagtaatt
     9841 ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc gaatcacttg
     9901 ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc aaaatataat
     9961 catactgact gcttacttct gctaaagatc cttttaatct tgattctctt gccatcattg
    10021 acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata ctcaaatttt
    10081 cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga acatcataga
    10141 tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt ccttgtgcat
    10201 ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct agattcaccg
    10261 tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt tgtgccatct
    10321 cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg ggtatttctt
    10381 tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga cattcggcaa
    10441 ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt gttttgcttc
    10501 tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt cttgtttagc
    10561 caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag cagtgacaag
    10621 gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt catgatataa
    10681 ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc gtttattcag
    10741 agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta tgctaggaaa
    10801 ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta cttcaaatgc
    10861 taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg taatagctgt
    10921 aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga attgattcat
    10981 ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt cttctggtat
    11041 cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc cctactttaa
    11101 cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg aaaagtaaga
    11161 aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga agagagaact
    11221 agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta cgtattacgc
    11281 tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga tacttaggtc
    11341 tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt ggattttctg
    11401 caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga tgttttctgg
    11461 aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt ttttgatgta
    11521 gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca attcttcatt
    11581 ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta aaaagtcgct
    11641 tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt cttggacttc
    11701 ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa ctgcttcttt
    11761 tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt gacccatttc
    11821 tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag cacgtgaagt
    11881 cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca tgacaccgat
    11941 gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca aagctgcatt
    12001 gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac catttgtttg
    12061 tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga gctggtgtgg
    12121 ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt tttctaatcc
    12181 agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag ataaaccttg
    12241 aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat gacgtggttt
    12301 gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc ctaccccttc
    12361 aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt ggtgagtcgt
    12421 ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac tatctggtgt
    12481 gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa cagaatagtc
    12541 gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga tagcaaagcc
    12601 aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg gacctgatga
    12661 atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg taataacaac
    12721 tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa caccatcttc
    12781 gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt tttcgatcgt
    12841 atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg ctcggacagc
    12901 tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga tattcttacc
//
LOCUS       DACEXQ010000057        12929 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.057, whole genome shotgun sequence.
ACCESSION   DACEXQ010000057 DACEXQ010000000
VERSION     DACEXQ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.057"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..182)
                     /gene="recO"
                     /locus_tag="IXH04_001176"
     CDS             complement(<1..182)
                     /gene="recO"
                     /locus_tag="IXH04_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAQ1851796.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKEIHRQ
                     NQPLAPALLPFTEAVY"
     gene            complement(325..1224)
                     /locus_tag="IXH04_001177"
     CDS             complement(325..1224)
                     /locus_tag="IXH04_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAQ1851797.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(1239..1640)
                     /locus_tag="IXH04_001178"
     CDS             complement(1239..1640)
                     /locus_tag="IXH04_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAQ1851798.1"
                     /translation="MPMDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFTVIVGLILFVPKIYHLLFN"
     gene            complement(1618..2094)
                     /gene="ybeY"
                     /locus_tag="IXH04_001179"
     CDS             complement(1618..2094)
                     /gene="ybeY"
                     /locus_tag="IXH04_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAQ1851799.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(2123..4315)
                     /locus_tag="IXH04_001180"
     CDS             complement(2123..4315)
                     /locus_tag="IXH04_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAQ1851800.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKAIDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNGKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(4339..5310)
                     /locus_tag="IXH04_001181"
     CDS             complement(4339..5310)
                     /locus_tag="IXH04_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAQ1851801.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            6015..7394
                     /gene="brnQ"
                     /locus_tag="IXH04_001182"
     CDS             6015..7394
                     /gene="brnQ"
                     /locus_tag="IXH04_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ1851802.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            complement(7550..7996)
                     /locus_tag="IXH04_001183"
     CDS             complement(7550..7996)
                     /locus_tag="IXH04_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAQ1851803.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(8023..8199)
                     /locus_tag="IXH04_001184"
     CDS             complement(8023..8199)
                     /locus_tag="IXH04_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAQ1851804.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(8361..8789)
                     /locus_tag="IXH04_001185"
     CDS             complement(8361..8789)
                     /locus_tag="IXH04_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ1851805.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            8881..9741
                     /locus_tag="IXH04_001186"
     CDS             8881..9741
                     /locus_tag="IXH04_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAQ1851806.1"
                     /translation="MSSDSTEIITFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLMQALQDALKEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            complement(9835..10128)
                     /locus_tag="IXH04_001187"
     CDS             complement(9835..10128)
                     /locus_tag="IXH04_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ1851807.1"
                     /translation="MPDTQKTKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(10201..11928)
                     /locus_tag="IXH04_001188"
     CDS             complement(10201..11928)
                     /locus_tag="IXH04_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAQ1851808.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDDEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAMRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIKKMTEE"
     gene            complement(12141..>12929)
                     /locus_tag="IXH04_001189"
     CDS             complement(12141..>12929)
                     /locus_tag="IXH04_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ1851809.1"
                     /translation="NYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKE
                     VICEDKTYEGKTVIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGG
                     GDSAVEEAIYLTQFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNE
                     MVVTGVKARNVKTGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTN
                     IPGVFAIGDVREKDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
BASE COUNT         3535 a         2760 c         2326 g         4308 t
ORIGIN      
        1 atataaactg cttcagtaaa agggagcaaa gccggagcta agggctgatt ttgccgatgg
       61 atctctttga tataaaacat cattttgcca gaagattctg taaagatttt gactaattta
      121 tctttttctt tgtaattacg gctagataaa atcaagcctt ttgattctgc aagctggctc
      181 atattctccc tcctaaaaaa taaatctgga aaagcagatt ctgccgatcc agatcattta
      241 tgttgcgatc ttccgcagct tttctcaaca cgcggatttt tattcagttt atttctatac
      301 ctaatttaca agaagctcag aatatcagta ttcgtctttt cggtagccga agtcttgcag
      361 atagactttt ttgtctcgcc agtctttttg aactttgacc caaagttcta gaaagacttt
      421 gtctcctaaa agattttcga tatcttttct tgctttcgtt cctacttgct tcagcatttt
      481 tccgcctttg ccgataatga tccctttttg gctatctctt tcgacaataa tcgtagcctg
      541 gatatggatt ttatcattct cgtttcgctt catcgaatca acaacgactg cgacagaatg
      601 aggcacttcg tctcgtgtta aaaacaagac tttctcacgg atcaattcag aaacgatgaa
      661 gtattcagga tgatcagtca cttgatcatc tggaaaatat tgtgggccct ctggcatttc
      721 actgactaat acatccatca aacgttcgac gttgtttcct tctgtagcag agattggaat
      781 cacttgtgca aaaggcatct gcgttgaata atcttcgatg attcccaaca actcgtctgg
      841 atggattttg tcgattttat tgataatcag ataaacagga gaatgcacgt tttttagacg
      901 ttcaataata aaatcgtctc cccgaccgcg tttttgatca gcactaatca taaataagac
      961 cgcgtccact tctttcaacg cactgtaagc tgcttctacc ataaaatctc ctaaacgatg
     1021 ctttggttta tggattcctg gtgtatcaat aaaaatcaac tgagcttctg gtgtagtgta
     1081 tactccttgg attttatttc tagttgtctg tgccttgtca ctcatgatgg caattttttg
     1141 acccacgatt cgattcaata atgtggattt ccctacattt gggcggccga cgattgcgac
     1201 aaaccctgat ttatgttctg gcatatatgt tctccttttt aattaaacaa caaatgatat
     1261 attttaggga caaataaaat caacccaacg atgacggtaa aacaagcggt cactaaaact
     1321 gctccagctg ccatatcttt gatttttttc ccaatcggat gaaaatgaaa atccgtaaac
     1381 atgtccacga cattttcaaa aacagtattg atgatctcta ctatccatac gagaaaaaca
     1441 gctaaaagca gccataacca ttctatccga tcaagttgga aaatcacgcc cataaacaaa
     1501 gcgaggattc cgaacgcaac atgtttcctc atgttgcgtt cttcctcaaa aacagttttg
     1561 actccttgga cggcaaattc cacggacatc aggaaatgtt tgtttttctc tacgttttta
     1621 tcttttaagt ccataggcat ctaagatctc tttctgtaaa ccaaacattt ctttctcatc
     1681 ctctggtgtc atatggtcat acccgttgat atgcaagaat ccatgaaccg ctaaaaagcc
     1741 aagttcccga tcatacgaat ggccgtaagc ttctgcttgc tctgcagcac gttcagtaga
     1801 gatcatgatg tctcccagta cacgaggcaa tggattctct ccatcttcaa aaatcactgg
     1861 gatttcgtct tcgccctctt cttccaaggc aaaactgatg acgtctgtcg gtgcatcttt
     1921 tccacggtaa tcacgattga ttacttggat tgctgcattg tccatgaaag taacagacat
     1981 ttctgtttct tctggcaact ctaaatatga tgctgcaaat tgcagcaagt cgtcgatttc
     2041 tttcattttc tcttctgaga cttggtttgt ttcatccatg aaggtaatat ccattataca
     2101 tcgcttcttt ctattcttct tgctattctt cttgttcttc ttttatcttc tcaacttccg
     2161 atttcatttt aggatatttg atccgagaat ggaaggtact gcttaaaccg ctgatcaatg
     2221 atttttctac tttacggatt tcttttaacg tcaatccact gtcgtccaat tgtccatcgg
     2281 aaatacgctc ttcaataaga ttatggacaa aactttcgat tttttcgctc gttggatggt
     2341 ccattgcccg tactgccgct tcacaactgt cagctatatt gacgattcct gcttcacggc
     2401 tttgaggttt tggtccagga taacggaatt gctcttccgt aacttccggg ttgcgttcct
     2461 ttgccttaac gtagaaataa cgcatcagcg tggttccatg atgctgtcta caaatatcga
     2521 tcaccatttg cggcatatta taatcttcca atattttggc accatctgtc acatgtccaa
     2581 agataatatg tttactgtct tctggcaata agaaattgtg cggattttcc gctcctgaag
     2641 gcaagttctc aacgaaaaag ctggcgtgtt tgatcttgcc gatatcatga taatagcaag
     2701 ctactcgagt tagtaaagaa cgtccaccga tttctgctac agcattagca cttagattgg
     2761 ctaccatcat gctatggtga tacgtacctg gtgcttcttc cagcaactgt ttcaataaag
     2821 gatgattggg attgctgagt tcatttaata cgatcacact atcatcattg accatcaatt
     2881 caatataggg gtgcagacct atcgtcagca agaatgaaaa cagtgcacct gtgaatccgc
     2941 agactagcgt taaccaagtc gttgcatccc caaagctcat tccttggtag ataatcaaaa
     3001 tgacatccat catcacagga aaaataatga cccacatagc agcggataac ccttgttctg
     3061 agatccgttt gcgtctgacg atcgttgcca tgagaccaga aaatacatag gacatcaaaa
     3121 taactgtcag aaagttcgtg ccaatcgaat cataaaaaat gaaaagtgcg gatactgctt
     3181 gaaaaatacc agccatgatc cctgctcttc gattcaagaa aaagcttaaa acaagtggag
     3241 caaaagccgc ggggtaaaac agcggaatat aagcagcttg ttctgtttgg aagagttgga
     3301 aaaatttcat tagtacgacg cttactgaca ttgccgtcac gtagaaaagt acgtattttg
     3361 ttctttctcc tgcattttgg aattgaagtg aattataaat cagtacaccg atttgaagca
     3421 aaacagctaa aaccatggca atgagtggga aaatagaagt cgtttgattc gtcaatccta
     3481 gtaattccaa ttttttcatc gctgaagcat caatttgatt tccttcgcga acaataattt
     3541 ctccttggaa gatcatcact ggttgtacag cttctcgtgc agattgtttt aattcttctg
     3601 tctttttctc attcaataca tcattaacta cgatgccttg atccactaag tagcgaatca
     3661 tttgttgctg ctctgttgtg acattcaaat attgaatctg atcttctgct tcttgtttaa
     3721 aagcttccaa ttcgctttca cgaacttgtt tactcatctg ttcgtcaatg agcttcaaac
     3781 tttcatccct aactgtgtga atttccgttt cagtcatttc aaatatcgtc gaataaaaat
     3841 tgttcggcag tttttggtaa aacgcaacgt tttctgcatt cacgttttca aaatttttct
     3901 tcagtgtagc aatccgctct tctgtagtag gtttcgggat attttcatct tccttcgcct
     3961 tatctacctt gccattataa gatttgttga tggtatcatt cgtcttatcg atcaattcga
     4021 ataattgctt gatccgattg ttctggtcat ctgccagatc tttttgataa gtatattctg
     4081 gagtcactgc ttctgctgcc agttttcttc tttgttcggt ttcttccgtg ttctcaatcg
     4141 ttttattcgc acggatactc tcttctgcta cttggccttc tctgaaatca atggcctttt
     4201 gtttgacact actaaatgta acgccgcaga caatgataaa aaacaagaac agaaggaaag
     4261 gcaatgctgc ctttccgagt ttttcttgta gtttctgcag tgttttattc aacatgtgct
     4321 tatcctccta agcaaagctt attcttgatg aagatccgcc ttttcataag ctttgatgat
     4381 ttcggcaact acagggtgcc gaacaacgtc acttgcttca aaattaacaa aatcgatttt
     4441 tttgatttgc tttaatgttc gttctgcatg aacaagacca cttgtcgtcc cacgaggcaa
     4501 gtctatctga cttgtatccc cattgacaat catttttgat tgataaccga gacgggtcaa
     4561 gaacattttc atctgagcga ctgtcgtgtt ttgtgcttcg tccaagataa cgaatgcatc
     4621 atccaatgtc cgcccccgca tgtaagctaa aggtgcgatt tcgatcacgc ctcgttccat
     4681 caatcggttc gtatgatcca atccaaaaat ttggtataaa gcatcataaa caggccgcag
     4741 ataaggatcc actttttctt tcagatcccc gggtagaaag cctaggtttt cccctgcctc
     4801 cacagcgggc cgcgtcaaaa tgattttttg cacttcgcct ttttttaggg cagcaattgc
     4861 taatactacg gcaagaaacg ttttccctgt tccggctggc cctacaccaa atacgatgtc
     4921 atgtttggct accgattcaa cgtatttctt ttgaccgctg ttttttaccc gtataggttt
     4981 tccattacgg tctttgacga tctcttcctc atacatctca acaaaataat ctaatgtacc
     5041 tttttgcccc attttcaatg ccgtcacaac atctggcgta cttacgtgga ttcctcgttt
     5101 gatcaattcc tgtaatgtcc gaatgactga ggcagccaag gaaatctggg attgctcacc
     5161 aatgatctga atgatttctc cacgtgtatg gatggttgtt tccgttgttt cctcgatcag
     5221 ttttatatgc ttgtcgtgtg cacctaaaag catgctgaca tcatcagcag ctgtcaatct
     5281 aatctctaaa gaactagact cttccgtcaa aaatagacat cccctttttt tcgattcatt
     5341 ctttcctatt ttaccatact cagaagaaac tatatagcaa ataagaaaag gttgtaaaag
     5401 agtcgcttaa ccatacgtac caagaaacgt taaaaaatag ccgtttccat tcgctaaagg
     5461 tctcgttttg ataaaaagaa gctgagaatg taagttatat gaaagtggag aacaaaatat
     5521 ttcgtttcat tcaacaagta ttctctaggt atttttcctg tttcaaaaaa agaaaacaaa
     5581 atagttaaaa tattcttgca tttcaaaaaa aacaggtgta ttattacgtc aacaaataaa
     5641 catacagcaa agcgtcgagg aaaacagtag gctattcaac gatttattcc cagtgagtca
     5701 tgtaagtgag aaatgacaat aaatcgaata gacgaacatc atttccgagt caatgtcgaa
     5761 aacgtagatc attgtggctg cctccattat caggctagcg gataacttta tcaaaaagcg
     5821 tccgtgaatg aagaagcttt tttaagcgaa aaaaggtggt accgttctag aatctagagc
     5881 ccttttccca agtggaaaag ggcttttttt tattttaagg tggtggtttt catggaatct
     5941 gatattagtg gcattactaa ttgctgcaac gtttatatcg tcaatcaaaa tgataattat
     6001 tggaggaaaa atttatggaa gaaacaaaaa cttattcatg gaaacaacta ttgatggtca
     6061 gttcacttat cttcgggatg ttcttcgggt caggtaatct aatctttcct gtccacttag
     6121 gtcaaatggc tggcgcaaac tgggtcagtg ctggcgtagg atttgctatc tcaggtgctc
     6181 ttttcccatt actagctatc ttagctgtag tagtgacaaa aagtgatggt ctgtatgact
     6241 tagctaaacc tgtcggaaaa tggtatgctg ccttgttctt aatccttgtt catttgacga
     6301 tcggaccatt tttcggtaca ccacggacag ctgctactgc ttttgaaatg gctgctaaac
     6361 catttttgcc tgaaaaatat aattcattag gtatgttttt attctctgct cttttctttt
     6421 tacttgctta tttccttact gtccatccaa cacggttggt caaatacgtc ggaaagtatc
     6481 taaactcctt gtttcttgca ttattagcaa tcatttttgt cgtttcttta ttccatccaa
     6541 tgggaaatct aaatcaatct gctgcagata cttatcaaac aaacgcttta acaaatggtg
     6601 tcctagaagg ttataacacg gttgatgcag tagctttgct tgccttaagt gtcacttttg
     6661 tccatgcagt ccgtgacctt ggtttcaaag ataaaaaagt cacagaatta acggcaaaag
     6721 ctggtagcct gagcgttgcg ctagaagtca tgatctattt tggacttgtt cttctcggtg
     6781 cgatgagttt gaataagatg tctttatcag aaaatggcgg aactgcactt tctcagatca
     6841 ctaattttta tttaggtaac ttcggcgcta gcttcttagg agtgatggtc acattaggtg
     6901 tcttcactac cgctatggga ctcgttgttt cttttgcgca agatttccat aaactgttcc
     6961 ctaaagtcag ttatatgaca tggctgcggt taacgacttt cgtctctttt gtcgttgcaa
     7021 atgctggttt agacaatatc atccaatggt ctttaccagt attgatgctt ttgtatccat
     7081 tgtcgcttgc gttgatcttg ctttccttaa cagcaaaatt tttccaaaag acaccatttg
     7141 tctatcaagt aacgatgttg tttgctgctg ttcctgccgt attggatatg ctggcaaatt
     7201 cgcctgcttt agtgagtcaa caaagagttg tagcaagcat gctcgaattt tatcatcatc
     7261 acgttccttt tgctgcatta ggacttggct ggatggtccc aacgttactt ggttacgctg
     7321 gcagcctact gttttattac gcctatcgcc tatcagggta taaacaagaa gcaaatgaac
     7381 taccagaaga ataaccaaat caaacacaat ttaacataag aaatattaaa caggaaaaaa
     7441 ccagctagat gaaagaatcc tattctttcg tctagctatt ttttagttct cttttttacg
     7501 cgcacaaaaa aactgcggac aatgattgtc cgcagtttaa atgataaaat taagattgta
     7561 atagttcttt cacaatggca tttacttggt ttccatctgc ttttcctttg actttaggca
     7621 tgatcgcacc catgactttg ccaaattctt tcattgatga agcaccagtt tgtgtgattg
     7681 cttcttgaac aagttgtcga atttcttcgt cggaaagttg ttccggcatg tatttttcga
     7741 ctatcacaat ttcacttttt actttttcag ctaaatcgtc acgtcctgct tcttcaaact
     7801 catgcaaaga atcacgtcgc tgtttcattt cgcgagacaa caccgttaat tcttcttctt
     7861 ctgtcaagtc gtgaccttct ttaatctgct cgttttgtat agatgacttg atcattcgga
     7921 tgacttgtaa agattcctta tctttcgctt tcatcgctgt tttcatgtca tcgtttaatc
     7981 tacttagtag tgacacacta ccaactccaa ttcatcacag aactagaatt ttttgcgttt
     8041 tctagctgct tcagattttt tcttgcgtct tacacttggt ttttcgtaga attcacgttt
     8101 acgagattct tgtaaagtac ccgcttttga cacggaacgt ttgaagcgac gaagagcatc
     8161 gtcaagtgat tcgtttttgc gaacgactgt ttttgacatg ttaattccct ccctccgagc
     8221 ttaaaaagta accgtaattg ttattgaaat atatctcaaa aaacaagact gtcctgtaaa
     8281 tattatatct aatgctgttt tgaccgtcaa ggaaatcttg ctttttttta aaattatcaa
     8341 atgttatttt atttaaatcc ttattgacaa ttttcacatc ttccgaaaat ctcgaatcta
     8401 tgcccttcga tcgtacatcc accgagctgt tcttcgaaat aattcattgg acacatatgg
     8461 atttctttcg ttgttccgca caccgtacag ataaagtgat ggtgatggcc gatttcccct
     8521 tgacagcaat gaaaacgaaa cttcatctct ccatttaaat cggtttcttc cagtaaatcg
     8581 atttctgcaa aatcatggag atttcgataa atcgtatcgt aacttactcc tttgaattcc
     8641 tgattcatat agtcaaaaac ttctttagcg gatacgtagc gattgcgctt cactaaataa
     8701 gttaacaatg cttctctttt tttagtatat ttaaaaccgt tatttttcaa tgtttctagt
     8761 gattgttcaa tcaatgtctg ttttttcatg acttattcct ccaaatcaaa atgattacga
     8821 agtacacatt ctagagtatg gtataataaa cataaaaaaa atgcaagtga ggttttatcc
     8881 atgtcttccg attctacaga aatcattact ttttttgtca tctctgactc tgctggagaa
     8941 actgcgacaa aattagcgca agccactatg gcccaatatc ctaccgttga gttcaattta
     9001 ttccgcagaa catttgtgac agatgagaaa acattgatgc aggcactgca agatgcacta
     9061 aaagaaaaag cccttgtcct acacacattg atcaatcaag aattgatcga gatgacgcgt
     9121 tctttttgtg aagaacatca attatttagt tttgatgtga tgactccgcc gatatcagaa
     9181 atcgagcgct taactggtat caagcccatt cggcaacctg gcgcccttca tttattgaat
     9241 gagaactatt tcaaacggat caaagcaatg gaatttgctg taaaatacga tgacgggaaa
     9301 gatcctcgtg ggtttctaga agcagatgtc gttttgctag gcgtatccag aacttccaaa
     9361 acgcctttga gcttgttttt agctaacaaa aatttaaagg ttgccaatct acctttgatc
     9421 ccacaagcac atattcccaa acagctttgg gagatcgatc ctaaaaaaat cgtgggactg
     9481 acgaataacc cagatatttt aaacaatatc cgtaaagaac ggatgcgctc ttacggatta
     9541 aatcctgata ctgcttattc cgatatcgaa aaaatacgag ctgaactcga ttttgcgaat
     9601 gatctttatg aaaaattggg ctgcgtagta atcaatgtcg cttctttatc tatcgaagaa
     9661 acagcttcac ttattttgaa tgctttagac ctagaagatc atagttatta tggaactgaa
     9721 accaccgaag aaacagacta acagtttttc ttgtcttttt cttaaaaaaa ctggactgtg
     9781 tcagcgaaga agattcgttg atacagtcca gtttgtctct atttgcacct atcgttattg
     9841 atgttccaca tgtttgagcg tctggttcaa aatatccagt acgtgagaat catcaagact
     9901 atacaaaatc gcttttccct cacgatggga cttgacgaca tggttttctc ttaataaacg
     9961 cagttgatgc gatacagcag attgttccat ttctactgct tgggtgattg ctgtcacatt
    10021 ttgttccccg tctttcagat acagcagaat tttgagacga gtggggtcac tcaacatttt
    10081 gaatagttgg ctgactttct ctacttttgt tttttgcgtg tctggcaatc cattcacctc
    10141 caacttgaaa aaagactgac ttctaaagtt taccactttt tccatcagtc tcaagttttt
    10201 ttattcttct gtcatttttt tgataatcgc ttcataagcg gctacttttt cagctgcgtc
    10261 ttctgaagag tttcctttta caccgatata aaatttgatt ttcggctcag ttcctgaagg
    10321 acggatagcg atccagccat tttcttccag cgtatacttc aatacgtcag atggcggtgt
    10381 cgttagtttt tctacgtttc cttcacggtc tgttctcgtc aactctttga agtcttctgt
    10441 ttggatgact tttgctccgg caaattctgt cggtgcttct tcacggaatt tttccatcaa
    10501 agcagagatt tttgcagagc cttcttgtcc gctcattgtc acagaaatcg ttttttctgc
    10561 gtagtagcca tatgtttcaa aaatcgtttg caagccgtca tacaatgttt tgccttgttt
    10621 tttatagtaa gctgctactt ctgctaataa gagcaatgct tgaatcgcgt ctttgtcgcg
    10681 aacgaaaggt ttaactaaat aaccgtaact ttcttcgaat ccgaacataa aagtatggga
    10741 atgatcttct tcaaattgtt gaattttttc agcaatgaat ttgaatcctg tcaatacatt
    10801 gaacatggta gccccatagt gttccgcaat tgctgtaggc aattcgctag aaacgattga
    10861 tttcaatacc gctgcatttt caggtaaagt gcctcgcgat tggtgtgctt ccaaaatata
    10921 ttgagcaaac agggctccga tttggtttcc tgtcaatact tcataggagc catttggcaa
    10981 acgaacggct gcgcctaaac ggtctgcatc aggatccgtt gcaatcagta agtcagcgcc
    11041 ttctttctcg ccaagtcgca tcgcatactc gaaagcagag tgttcttctg gattcggtga
    11101 tttgactgta gtgaaatccg ggtctgcgac agcttgttct ggaactagta cgaattgttc
    11161 gaatccagct tgtttcaacg cacgttcgcc caacattttt cctgttccgt gaagtggtgt
    11221 gtacacaagc ttcaaatctt tacccattgt ttccaccaat tcatgatcaa tcgttactgc
    11281 tttgatttct tctagataag cggcatccac ttcttctccg atgatcgtga tcaatccgct
    11341 atgttcagct tcgtcgtcag ataatacttc aacttttaac ggattgctga cttcacggac
    11401 atatttcgtc aatgcatctg cgtctgcagg aggcatttgt cccccatctt ctccgtagac
    11461 tttatatccg ttgtaagctg ctggattgtg ggatgcagtt accatgattc ccgcaaacgt
    11521 cttcaaatga cggactgcaa aagaaagctc tggtgttgga cgtaaacttt cgaatacata
    11581 agaagggata ttgtgatgtg ctaatgtttt ggctgcttcc atggcaaatt ctggtgattg
    11641 atggcgtgag tcataagcga tagcgacacc tcggcgtttt gtttcttcac cttgtgtatc
    11701 catgaaacgt gcaagtcctt ctgttgcttg tcttaccgta tagatgttca ttcgattgat
    11761 cccaggtcct aagattcccc gcatgcctgc agtaccgaat tccaacggtg cataaaaagc
    11821 atcttcaagt ttttcaggat cttcacccag atcatttaat tgttttttta agttttcttc
    11881 taaattttct tcatgtaccc attgttcata cacttgttcc caagacatga aaataacacc
    11941 tctttctgaa atatggttct taataagtat agcattcact tattagatat gaaagtatga
    12001 ggaccaaaaa acgcttaaat caagaaaaga ttcttttatc aagcacggtt acaagtattc
    12061 gcataaatcg gattcagtag atttcacgca aaaaaaagag taaaagaaca attgaaaccg
    12121 ttctttcact ctttttaaac ttatgcagtt gtttcttcta aagattccaa gtatttatac
    12181 acttgttgtc cagcaatccc accatcacca acagcagtag tgatttgtcg caaatctttt
    12241 tcacgaacat ctccgatggc aaaaacgcct ggtatattcg tcttcatttc gttgtctgtt
    12301 ggaatccagc cggcttcgtt cgtcaaccca gcttctctaa atggtgcagt taatggatcc
    12361 aatcctacat agatgaatac tccgtctgct gccaaatcaa actcattgcc agttttgaca
    12421 ttacgtgctt tcacacctgt cactaccatt tcatttccaa cgatttcgtc aacgactgta
    12481 tcccacataa aggaaatttt ttcattcgca aatgcacggt cttggatgat cttttgtgca
    12541 cgtaattcat ctctacggtg gacgatgact acttcagaag caaattgtgt caaatagatg
    12601 gcttcttcta ctgctgagtc tcctcctccg atcactacta gacgtttgtt acggaaaaat
    12661 gcaccatcac aaactgcaca gtaagaaacg ccacgtccag caaattcttc ctcgccaggt
    12721 acgcctaatt tacggtggat acagcctgtt gcgataatga ctgtttttcc ttcgtaagtt
    12781 ttatcttcac aaataacttc tttgtggctg ccgtgatcct tgatatcttg cacgattcca
    12841 taagcatttt ctgtcccaaa tcttgaaaca ccttcataca tcttataagc tagatcagga
    12901 cccatgattg aatcgaaacc aggatagtt
//
LOCUS       DACEXQ010000058        12860 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.058, whole genome shotgun sequence.
ACCESSION   DACEXQ010000058 DACEXQ010000000
VERSION     DACEXQ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12860
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.058"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..259)
                     /locus_tag="IXH04_001190"
     CDS             complement(<1..259)
                     /locus_tag="IXH04_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAQ1851810.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQIT"
     gene            complement(252..791)
                     /locus_tag="IXH04_001191"
     CDS             complement(252..791)
                     /locus_tag="IXH04_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAQ1851811.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            complement(927..1145)
                     /locus_tag="IXH04_001192"
     CDS             complement(927..1145)
                     /locus_tag="IXH04_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAQ1851812.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            complement(1142..1387)
                     /locus_tag="IXH04_001193"
     CDS             complement(1142..1387)
                     /locus_tag="IXH04_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ1851813.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            complement(1503..2231)
                     /locus_tag="IXH04_001194"
     CDS             complement(1503..2231)
                     /locus_tag="IXH04_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAQ1851814.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            complement(2390..3433)
                     /locus_tag="IXH04_001195"
     CDS             complement(2390..3433)
                     /locus_tag="IXH04_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ1851815.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            complement(3423..3914)
                     /gene="coaD"
                     /locus_tag="IXH04_001196"
     CDS             complement(3423..3914)
                     /gene="coaD"
                     /locus_tag="IXH04_001196"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAQ1851816.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            complement(3911..4471)
                     /gene="rsmD"
                     /locus_tag="IXH04_001197"
     CDS             complement(3911..4471)
                     /gene="rsmD"
                     /locus_tag="IXH04_001197"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAQ1851817.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            complement(4590..4928)
                     /locus_tag="IXH04_001198"
     CDS             complement(4590..4928)
                     /locus_tag="IXH04_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAQ1851818.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            complement(4912..5349)
                     /locus_tag="IXH04_001199"
     CDS             complement(4912..5349)
                     /locus_tag="IXH04_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ1851819.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            complement(5339..6463)
                     /locus_tag="IXH04_001200"
     CDS             complement(5339..6463)
                     /locus_tag="IXH04_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851820.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            complement(6484..9912)
                     /locus_tag="IXH04_001201"
     CDS             complement(6484..9912)
                     /locus_tag="IXH04_001201"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAQ1851821.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            complement(9938..11101)
                     /locus_tag="IXH04_001202"
     CDS             complement(9938..11101)
                     /locus_tag="IXH04_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ1851822.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            complement(11104..11406)
                     /locus_tag="IXH04_001203"
     CDS             complement(11104..11406)
                     /locus_tag="IXH04_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAQ1851823.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            complement(11520..>12860)
                     /locus_tag="IXH04_001204"
     CDS             complement(11520..>12860)
                     /locus_tag="IXH04_001204"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HAQ1851824.1"
                     /translation="DFRPSYLQMANQLDQLPNRPVIVALTATATVQVAADIKRLLKIP
                     ENNHIQTGFERENLRFQVIKDQKKEQYLIEYLKINKNQSGIIYAATRKEVDRLYHLLK
                     KFDFSVGRYHGGLNENERTEMQEAFLYDRLQLIVATNAFGMGINKSNVRFVIHYQIPG
                     SLEAYYQEAGRAGRDGLSSEAILLFSPQDIQVQKFFIQQSQREEGQKQKEYEKLKAMT
                     EYVYIESCLQQYILNYFGETSSPCNRCGNCLDDREIVEVTTEAQMVLSCLKRMGENYG
                     KQMLMKVLAGSKEQKLQALGFGHLSTYGLMKNQSQKETMQLIEYLISNGYLLTINGEY
                     PVLKVTERGIQVLKGQESVYRKEPKKVQQLSDEETDTLFEVLRELRTDLASEAGVPPY
                     VVFSDSTLKEMSRIRPSSRLEMLQIKGVGQSKLDKYGEAFLSRIKNADSNVLDK"
BASE COUNT         3522 a         2796 c         2187 g         4355 t
ORIGIN      
        1 cagtaatttg cgcagatgtt gttttttcct ctaaaaatga atactcttgt agcctataag
       61 aaatggatac cgttatgcta cacaaaatac acaagtacgc gataaaacgt ccttctttta
      121 gcgaaaacca taaaaaaatt ccggcaagaa gcaaacgata accccaattg tcagtgtaat
      181 acaacaaaca gataatcatc gacgtaattg ccggaaaaat caaccgattt cgaacatgcc
      241 tttctaaaac gctagacatg ttttcctgtt aattcatttt gaagttttga aaaatgctgt
      301 gagtctaaag caacttggtg tacagataca cctaattgtt cgatcaacgc ttgggcataa
      361 ggatcatttc gatagtcatg gagataattg atccctttca caccagcttg caacaaagcc
      421 tttgtacagg ccaaacatgg aaaatgtgtg acatagattt ctgaaccttc cgtcgggata
      481 ccaagttttg cacactgtag gagtgcattc atctctgcat ggatcgttcg caagcagtgt
      541 ccatccacga cgtaacaccc ttcatctatg cagtggacat ctccactgac tgctccatta
      601 tagccgccag caatgatcct tttttctcga acgagtgtcg ctcctacttc taaacgtgta
      661 cacgtactac gtaaagataa cagtactgcc tgagccataa agtactggtc ccaaggaatg
      721 cgttcattat gtgacttatc agtaactttt tctttattcg tatcgcatgc ttttgaagat
      781 ttttctaaca tgttttatat tcctccgttt tttcaagttg attaaaaagt actcattgct
      841 taaacaaaat attctttaaa tataacgaat catcctgtct gacaaaagat cctacccttc
      901 actgccgaaa tgaagtccgt catccattag cccattataa aaattcttcg aatccatttg
      961 ttcaagaaat tccgtataaa aatcgatact tcacaatcat ggcgttttgc tacttctgtg
     1021 agaacagaga tatattcttt gatcaacttg ttattccgat atttttgttt tgtctcatcg
     1081 actgctggtg gcgtgataaa gaaagcatga tcagaactat agtctttttt cagctgaact
     1141 atcatccact gcagattttt tggtacgttt ctttagaaag ttgtttatgc tctgctagat
     1201 cattcgtccc aatgagaata aatactttat ctgctgtata gctaagtaca tctttggcaa
     1261 agcgttctcg tagagcttga ctgttattgc cgctgacagc cgtattcata atatgaatcc
     1321 cagatatttt attttggaga tagacattca gcattggttt ttttaatgtt tctttgcgaa
     1381 caatcaagct atttcctatc caaaaatact acctcctcgt cctgccaagc tcttagaatt
     1441 ggattattct tagtatccat tagcgtccat ttattgctcg tgccagtcga ttttgaatca
     1501 ttctatttaa ttgtaacgaa gttttttagt ttttcaactg tcttttcacc gataccagaa
     1561 atctcctgca ggtcttccac tttctggaag gatccgtttt cttctctaaa tcgaatgatt
     1621 tcttgcgctt tgactgatcc gatcccagga atctgctgaa gttctgatgg agtggctgta
     1681 ttgatattta ttttgggagg ctcggaatct gctgtaggct gttttccttc cgaggatgcc
     1741 aactgatcaa gatgttctgg cagctcttca cctacagcag ggatatatag gacttgctga
     1801 tcttctatct tcgcagaaaa gttgattcga tcgctatccg cttcttctaa taatccacct
     1861 gcacgcttta gtacatcata gacccgttct tctgaagaaa aggaatagat accaggctcc
     1921 ttcacacttc ctttgatatc ggtgtacatg acctttggac gagcatcctc ttctacatcg
     1981 tttccttcag ctgatggatc ttctccactg gaactaaacg aagaaagtat ttcttttgca
     2041 gcagtctctt ctgtttctct ataaaatagc aaatttgata ataaaaaacc acttgcgata
     2101 caaaagataa gaactatcat agacatccaa atgatcaatt tgggtgaagc tagtattttc
     2161 ttgctgattt tttctatcat tgttcttttt tcaaagaatg accagtaaaa atcattcatt
     2221 actcctttca tttaaaattg aagaaacagt gagctctggc aaaatcgtcc cttcaacatc
     2281 taattacgtc aattttttct tttgttttta tttttatcgt gaagattttt gacgaaaaag
     2341 aggggttgtg acagaactcg tgtcacaacc cctctttaat tatttagttt catttcattt
     2401 tctctagata gtcaagcgct tcttgtactg tttttactgg tacgattttc attgaggaat
     2461 tcaatttttt agcggcagct tttgcttctt cgtagttggt ttttatttca ggatattctt
     2521 tcttaacatc gtctgaaatc gtatcatctg gtgcaaagaa aatctctacg ccagctttat
     2581 ccgcacttgc cactttttta tcgattcctc cgattcggcc aacttctcct tcactgttga
     2641 tcgttccagt tcctgcaatt tgcttaccat gtcgcaagtt tttaccagtg acttgttcat
     2701 agatttctaa agtgaacatc aatcccgctg atggcccacc gatagagcca gaattaatac
     2761 gaacatctgt atctgattcg atctcggtat ggtcagtaag cgtgatacct attcctgcct
     2821 ttttatctgt tggtaactcg atcagatcac cagaagctgt tttttcttca ccattatgca
     2881 aataactgat ttcgattgtt tgtccaactt tttggctttt tacataatcc ataaatgctt
     2941 ctgtactttg aaagcttttc ccgtctactt ttgttactgt atctccaact gaaagcttgc
     3001 caaagaaatt cgatgttttc tctacactca tgacatatac gcctttgaat tccattttat
     3061 aaggcttgtc tgcaagtttt aacgcttgtt cgattgctgc attttgggaa gaatccatat
     3121 aatactgttg catttgatta tattcttcat ttgtcgcccc accagtcaat tcttcttcac
     3181 tgatcaattc ttcaaaaggg gtcattttag cttttaaagc taagaaacct gtagcttgcc
     3241 tgataccaac agaggtaaga gagaaagacc cagaaccttt atcttcctta tcattgaccg
     3301 taattagttc tttcagatcg atcgtggctc ccggtgattc tacataatac ggcaagggaa
     3361 ctactacaaa tccaatgatc aacattccaa atatgataat cagtactgtt ttcaaccatt
     3421 gtttagcttt catttgattc acgctttcca gctaaagctt cgttgatcac tggcggtaag
     3481 tagctgctca catctccgcc aaagtgcagt acttctttta aaatactgga actcacgtga
     3541 ctatattctg gttttgctaa taagaagaca gtttccaatt catcatatag atgatgattc
     3601 atctgagcaa tctctcgttc atattcaaag tctttgatac ttcgtatgcc tctgatcaac
     3661 gcatctgcac caatctcttt tgctgtttct accgtcaatt gattttcttg atgcatgact
     3721 tttacattag gaagatgaga aacagctttt gtgatcaatg tcattctttc ttctggggga
     3781 aacaaacttt tctttgaagt attgataaaa acaccaatta ctacctcatc gaataattta
     3841 gccgcacgtt ccaccgtatc taagtgacct tttgtaaaag gatcaaaact tcctgggaat
     3901 aatgcacgtt tcattgattt ccctcttttc tatagatagt tacggccgtt atgccataaa
     3961 tctgttttct tatttgatgg aatccagcca cagtttctgg caattcgact gttttatccg
     4021 tttcgcatac gataactgcg gttttagaaa aaagtcccag ttcttccatt ttatggattt
     4081 gatcgattat tttttgtttt gcatatggcg gatcaagaaa caagtagtcg aattgttgtt
     4141 tctccgctgc tagccgttcg attgctttgt ccgcatcaag ttttattgtg gtaaatttct
     4201 ctggttcttt cgtaatttcg atgttttctt tgatgatttt tagcgcttgg taatttttgt
     4261 ctacgcatac cgcatgactg cagcctctag atactgcttc gatagctaaa ccaccgcttc
     4321 ctgcaaacag atccaatact gtctctccat caaaatatgg tccaatcata ttgaatatgg
     4381 attcttttac tttatctgta gttggtctgg tgttcgcccc tgctaaactt tttaaacggc
     4441 gcccgccata atttcctgat attatccgca tctactcact ccttagaaat ggtactggta
     4501 ctagtatacc aaaaaaatac ttggacaatg atcaaaaatc gatcgttatc caagttattt
     4561 tttatttaaa aactgctcat aagagatttt taaagatgtt cagctaatcg gatttctgta
     4621 tttagctctt caatttcgaa accttcgtct tttaatactg ttttgtttcc tattttttcg
     4681 gcaaaattca tttcgatatc cggtcggtaa gaacgttcta cgtttcgaac aaaatgtaat
     4741 ttacggattc gttcttctgt ttcttccaat gtctcttcat ttaagtaaag cacgacatat
     4801 ttcattcttc ttgagacata atgcaacaag ccgaactttt tcagattctt taattgtttt
     4861 aagctgtaga cccatacgat cagcccgcgt cgttttgtta tttcaaattc actattgtct
     4921 aattgcatga caattacctc cgcatccaaa gtgttttttc gtttcaaaaa acgggctacc
     4981 tgcatctact ttgatttcat tagaaacttc ttcggcaagt acttggacaa tttgatccaa
     5041 cagactttgc agctgtgttt cactatatcg gaaagctgca acatgctcat ccatatcaag
     5101 ctgtcttttt gcttttcgaa cagctcgttt tttttcacga taatcaggtg catactcacc
     5161 atatgccgct acattttcaa atgcttcttt cttagacaag aacgcttctt tcaactggca
     5221 aacttttgca gattcttcca ttgactgttt atgttcaaga taagaagcca tcgttttact
     5281 cttttttatc atttcagcga cttggaggca ttgatcctcc agttgaaaca gctcttcgtt
     5341 aacgatcact gctatcacta ctttctccgt tttcttcttg caataagact aacatatttt
     5401 ctttagaaaa agcaatgcct acagaatccg ttcccgggtc catgaaacgg tcatggttgt
     5461 aaggatcgct gtaccaatat aaaaacgaaa aagtagcgtc aatcgccgga tgaataaata
     5521 gcccactagt attttctaat ttttgtttac tgattaactt ctccaattca tcattcgtca
     5581 gtgagaattt cactgtcgtt tgagcactgc ctagcgattt ttgccaatca gccatacgtt
     5641 cctctgacaa tatttcctca ggccttgtca aaaaatcatg tagtagcagc tgactgtggt
     5701 tttgaaaggc aagtgtttga ttgtatatgg gaagttcgtc ttctcttcta agcatattta
     5761 ataaagtcgt actttttcgt tctttaacgc gattgattga ttcccaatca gccgatttat
     5821 ctgcttgata atgtggaagg ccttcaccaa acacttgata tggcaatagt ttcaaaagac
     5881 tatctttatc taaatacaca actgaaaata ggccgtttgt tgtttgatca aaaaataaaa
     5941 tagcaaaagt gtcgttatca aaagcaatta gcggacgata attcatatcc tcttccatta
     6001 gttcgattcc aacttctgta tcatggtact caaaagtgaa attagtatag atagtagtga
     6061 ttttcgtaag atcttgcatc gtcatcccta aataaaacgg cgcgatatca gcaggccctg
     6121 tttgcagtac tttaacagtt gtgactctgc tgtcttggat gttcgcttca aatgtgagat
     6181 gactagcttt attttcataa tatcttgttt caaaaccaaa tccgctagtt aatcgatcat
     6241 aggcttggcc aaaatccttt tccagctgat cgatcggttt tccgatataa gtagaaaaac
     6301 cagttgcttt aagctcctgg tatttccacg ttgtcatttg gtttgatatt tgttgatcag
     6361 agttttcctt cacttcaagt tttggcggaa acaagactgg ttccatgtag aatgccacaa
     6421 taactaatag cagaatcagc caaaacaaac ccagtctttt catgtttccc cacctttcgc
     6481 ttattatttc tcagtcactt caatcaaaag gtcaccagac gaaatagtgt ctcctgaaga
     6541 cacatataca tgtgcgactt ctccgtcaaa acgtgcttcg atggccgttt ccattttcat
     6601 cgcttcagta atcatcaacg tatcaccttt tttaacgtta tcgccttttt tcactaatac
     6661 atccaaaaca gaaccagaca tcgttgctcc aatttgttct cggtttgtcg gttcagcttt
     6721 caatcgggtt tggaccgtac tgatgatcga attatctttg atagtgattt cacgtctttg
     6781 tccattcaaa ttgaagaaca gtacgcgatt cccttcgata tcgggttctc cgatttcatc
     6841 taaacgaatg ataagcgttt ttccgcgttc gatttgtaca ttgattgttt cacccaaacg
     6901 tataccttgg aaaaatgttg gtgtgtccag taatgtaaca tcgccaaatt gattatatgc
     6961 tttttgatag tccaaaaaga cttgcggata catcaaatag cttaatactt cgtcttgttt
     7021 tggttcatag ccaatttttt ctgccaattc tttttttact tcgttgaagt cgactgattc
     7081 agccaaaaga cctggacgtt cattcaatgc aggtctgcct ttaagaataa tcttttgcaa
     7141 ttttttagga aatccgccaa cgggttgccc tagttctcct tggaagaaag tcacaacaga
     7201 ttcagggaaa cttagtgttt cgcctttttc ataaatatct tcttctgtaa gatcattttg
     7261 gaccataaat agtgccatat ctccaactac tttagatgat ggagttactt taacgatatc
     7321 gccaaacatc aagttgacag tatgatacat ttgtttgatt tcatcccagc gatgacctaa
     7381 tccgacagct tttgcctgtt gctgtagatt cgaatactgt cctcctggca tttcatgcat
     7441 atatacttct gtttcaggag cattcaagcc gttttcaaac ggtttgtaat acatgcgcac
     7501 atcttcccaa taatggttta atttctgtgc attttcaatc gtgatttctg gcagccgtgg
     7561 accattgacc agtgcataat acaagctgct catgctaggc tgactagtat tcccactcat
     7621 tgcactcatt gccacgtcaa cgatgtctac tcctgctttt gaagcagcag aataagtgat
     7681 gattccattc ccgctggtat catgagtatg cagatggatt ggcagatctg tcgtttcttt
     7741 caattcacta atcaaacgat aagctgcctg cggtttaagc agcccagcca tatctttgat
     7801 tgcaatgata tgcgcaccca gttgttctag ttcctttgcc atgtctttat aatactgaac
     7861 attgtatttt gcccgacttg gatcgttgat atcgccagta tagcaaatag ctgcctctgc
     7921 gattttacca gtatcccgta cagcttggat acttttttcc atttgtggag tccagttcaa
     7981 actatcaaag attcgaaata catcgattcc ttgcgctgct gcttctttga caaattccac
     8041 caatacattg tcaggataat tagaataccc aacggcattt gaacctctga aaagcatttg
     8101 cagcaacgta ttaggcatta aagaacgaat ttttctcaac cgttgccaag gatcttcatt
     8161 caaaaagcgg taagccacat caaatgttgc tcccccccac atctcacttg aaaagagttc
     8221 aggaagacct tctccagtca aacgtgcaat ctgcttgaaa tcctttgtcc gaacacgagt
     8281 tgctagcaga ctttgatggg cgtctctaaa tgtcgtatcc gtcaacaaca aattttcctg
     8341 tcctttgatc cattcgacca aagcatcggc acccttcgca tctaatacat tcttagccgt
     8401 tacgtaatca gttcttgtga ttagatcttt aggcatacgt ggttcttcat aaaatggttt
     8461 ttcgccactt tcgatacctg gaaaaccatt cacagtaatt tcaccgatat atttcatcgt
     8521 tttgtttccg cggtctcgta gtcgaggaaa ttcaaataat gttgctgtac tatcaatgaa
     8581 ggttgttttt gcatttcctg attggaattc aggatgtgtg atgacattga gcataaatgg
     8641 aatgtttgtt ttcactccac ggattctaaa ttctttcaag caacgttcca ttttctgaat
     8701 ggctgtttca aaggtagcac catgcgtaca tacttttact agcaatgaat caaaatacgg
     8761 tgtcactaca tagcccgcgt aagcatttcc gacatccaga cgtacgccaa aaccaccagg
     8821 tgaacgatac gtatcgattt tccctgtgtc tggtaaaaag ttattcaacg ggtcttctgt
     8881 agtgattcgg cattgaatgg ctgaaccatt taatttaatt ccttcttgct gaggcaatcc
     8941 gatttcttta tgcagatcca aaccttgagc aatcaacagt tgcgttgtta cgatatcaat
     9001 atctgtaatc atttctgtga tcgtatgttc cacttggacg cgagggttga cttcgataaa
     9061 gtaaaaatca tctccttcaa caagaaattc tactgtacct gcgttgacat acccgacata
     9121 tttcatcaat tggacagctg cctggcagat tttttgacgt tgctgttcat tcatagagac
     9181 acaaggcgct acttctacta ctttttggtg acggcgctga acagaacaat ctctttcaaa
     9241 aagatggatg acgtttccat gcgtatcccc gagaatctgt acttcgatat gcttaggatt
     9301 agcgatgtat ttttcaacat acacttcatc actaccaaag gctgcttttg cttcgctttt
     9361 cgcacgttcg tagccctctc ttgcactctt ttcatcgtga gctacacgca tgccgcgtcc
     9421 gccgccgcct agagctgcct tgatcataat cggataaccg tattgtttcg caaactccaa
     9481 ggcatcgtct atagaagcga ttggtccatc tgtgcctgga atcgaagcaa tccccgcttc
     9541 gatagctgca gcttttgctt tgattttatc tccaaaaata tctaaatgat gcagtttggg
     9601 tccaacaaaa atgatgcctt cttcttcgca ccgttgcgca aaattcaagt tttccgacaa
     9661 aagaccgtaa cccggatgaa tagcgtcagc tccgcattct ttcgcgatag aaatgatccc
     9721 ttcaatatct agatacgcat caatcggttt ttttccttgt cctactaaat atgcttcatc
     9781 cgctttgaaa cggtgtacgg aatattcatc ttcttctgca tagattccaa cagttttaat
     9841 tcctaattct gtacaagcac gaaatacacg tacagcgatt tcacccctgt tagcaactaa
     9901 aacttttttc atcgaaccac tcctacatct ttttttatta taaacctaag ctcttgcgtt
     9961 tttcatccgc actgatgttg aggacaaagc cgacacaaat cgaaagcatc agcaaactgg
    10021 agcctccttg acttaaaaat gggaacgtga tccctgtcaa aggaataacg ccagtaattc
    10081 ctccaagatt gacaaatact tggatcaaaa acattgcgcc gatcccaatg cacaaaagcg
    10141 aattgaacgg atcttttgaa cggataccta caaggacaat gcgcacaatc ataaagaata
    10201 gcaacgctaa tatcaaaatc cccataatca ctcccagttc ttccactacg attgcataaa
    10261 taaaatcggt ttgtgcttct tgtaaaaatc cttttttctg gatactgtta cctaatcccc
    10321 tgccaaatag tcctccgttg aacatggcat agtagccgtt gactagttga tgccctttat
    10381 ttaattcgtc tgagaaagga ttttggtaaa tagcaaatcg attatagata tactgtaaac
    10441 gtccgggtaa gatctttccg tttgtgatat tgatcagcca gatcgtaaaa gtacttagac
    10501 aaaagcctcc tactcctaca atcaaagtat acacataatt gactccgctg gctaaaagca
    10561 gaaccaaaat aatcaaaaag ataactgccg cattaccaaa atcgggaaga atagcgacta
    10621 atgcagtaag tccgataacc attgcaagtg gacgtttcac agtcccaaga aaatcttttt
    10681 gtacactgtt ttgtctttta gataaagtca gagacaggta ccagatagaa atgattttta
    10741 ggtattcggc aggttggatc gagaatcctg caatttgcag ccaacctttc gccccattga
    10801 tctccttacc aaaaaacaca acaatcagca gcaaaatcgt caaaactgcg atggctgcca
    10861 tgatcaaacg ctggtttttc aaaacatctg ttttcatttt ataaagtagt gcgatagcaa
    10921 tcaagctaag cacccaaaaa atactctggt taatgactga tgcggacgga ttttgtccgt
    10981 tttctaataa tagatacgac gtagaactat ataccatgat caatcccaat acacataaaa
    11041 ttaaataggg aatcaagatg ctatagtcta atagatgcct ttttttcact ttcttaggca
    11101 agtctattcc tccttgccaa cttctttttt atcataaatt tcttggtaaa cctcactata
    11161 taactgattc aattctcgtt ctagctcact taacatcagc tggccctcgt tctttccgat
    11221 tactcctata cggacagcaa aaccgacttg ccgggaaaag ccgtacatct gtgtatctac
    11281 cacttcttcg aaggctttgc attgtgagat acagagactg ttgttttgat ttcggattaa
    11341 tcgtttgatc cgttctgctt catcatttaa tacttggatc gcaagttttt tcgacaaaga
    11401 atccatctgt gcttacctcc cattctaatt ccttaattat aacatatttg attttcataa
    11461 ggagcaaaaa aaacagattt aaccagttgt ttttattcat ttttacgtat tcgttctttc
    11521 tacttatcca gaacgttaga gtccgcattt tttatccgag ataaaaaggc ttcgccatat
    11581 ttatcaagct tgctttggcc aactcctttt atctgaagca tctccaatct gcttgatgga
    11641 cgtattcggc tcatttcttt caatgtggaa tctgaaaata cgacatacgg gggaacgcct
    11701 gcttctgatg ctagatccgt tcgcagttcc cgcagcactt caaatagtgt atcggtttct
    11761 tcatccgata gctgctggac tttcttgggt tcctttcgat aaacagattc ttgtcctttc
    11821 aatacttgta tccctctttc tgttactttc aaaacaggat actcaccatt gatggtcaga
    11881 agatagccat tcgatatcaa atattcaatt aactgcatcg tttctttttg cgattggttt
    11941 ttcatcagtc cataggtaga taaatgtcca aaacctaaag cttgcagctt ttgttctttc
    12001 gatcctgcta agactttcat cagcatttgt ttaccatagt tctcacccat acgttttaag
    12061 caagaaagaa ccatctgtgc ttctgtcgtt acttctacta tttctctgtc atctaaacag
    12121 ttaccacaac ggttgcacgg actgcttgtc tcgccaaaat agtttaggat gtattgctga
    12181 agacaggact caatatacac gtattctgtc atcgctttta atttttcata ttctttttgc
    12241 ttttgccctt cttctctttg ggactgctgg ataaaaaact tttggacttg gatatcttga
    12301 ggagagaaaa gcaggatggc ttcactggaa agaccatctc ttcccgcccg accagcttcc
    12361 tgatagtatg cttctagaga gccaggaatt tgataatgga taacaaaacg gacattgctt
    12421 ttatttatac ccataccaaa tgcatttgtc gctacaatca gctgcaagcg atcgtataaa
    12481 aaggcttctt gcatttctgt tcgctcattc tcatttagac caccatgata tctacccacg
    12541 gaaaaatcga attttttaag caaatgatag agacggtcta cttcttttct cgttgctgca
    12601 taaatgatac cagattgatt tttgttgatc tttaaatatt cgattagata ttgttctttt
    12661 ttttgatctt taattacttg aaatcgtaga ttttctcgtt caaaaccagt ctgaatatgg
    12721 ttgttttctg gaatttttaa aagtcttttt atgtcagcag ctacttgcac agtcgctgta
    12781 gctgtcaaag caacaattac aggacgattt ggcagttgat ctagctggtt cgccatctgt
    12841 aaataacttg ggcggaaatc
//
LOCUS       DACEXQ010000059        12809 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.059, whole genome shotgun sequence.
ACCESSION   DACEXQ010000059 DACEXQ010000000
VERSION     DACEXQ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.059"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..790)
                     /locus_tag="IXH04_001205"
     CDS             complement(<1..790)
                     /locus_tag="IXH04_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1851825.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTR"
     gene            complement(846..3326)
                     /locus_tag="IXH04_001206"
     CDS             complement(846..3326)
                     /locus_tag="IXH04_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851826.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(3310..3645)
                     /locus_tag="IXH04_001207"
     CDS             complement(3310..3645)
                     /locus_tag="IXH04_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851827.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(3659..4693)
                     /locus_tag="IXH04_001208"
     CDS             complement(3659..4693)
                     /locus_tag="IXH04_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAQ1851828.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(4783..5463)
                     /locus_tag="IXH04_001209"
     CDS             complement(4783..5463)
                     /locus_tag="IXH04_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ1851829.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(5747..6850)
                     /locus_tag="IXH04_001210"
     CDS             complement(5747..6850)
                     /locus_tag="IXH04_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ1851830.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(7479..7949)
                     /locus_tag="IXH04_001211"
     CDS             complement(7479..7949)
                     /locus_tag="IXH04_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAQ1851831.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            8279..9919
                     /locus_tag="IXH04_001212"
     CDS             8279..9919
                     /locus_tag="IXH04_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAQ1851832.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            10073..11395
                     /locus_tag="IXH04_001213"
     CDS             10073..11395
                     /locus_tag="IXH04_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAQ1851833.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(11463..11894)
                     /locus_tag="IXH04_001214"
     CDS             complement(11463..11894)
                     /locus_tag="IXH04_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1851834.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEA
                     KKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(11959..12795)
                     /locus_tag="IXH04_001215"
     CDS             complement(11959..12795)
                     /locus_tag="IXH04_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAQ1851835.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
BASE COUNT         3743 a         2618 c         2325 g         4123 t
ORIGIN      
        1 aacgggtcaa aaatgtatgg aggtccaata aaaattgtgt cgtttgaaac aatccaacag
       61 gaagattgct ataatctggt ttggattttt tcgtacagcg cattcgcttc ttcataatgg
      121 aaagccatat ttcaaatttc aatcgtcctt cttctgtaat agtaaaacta aactcttttt
      181 gcagcagatc taaaaaagtg tttcctgata aatcactgta ttcttttttg ttttcaagat
      241 aaggacgtcc aaaccaaaat aattggaaat aaaaataaca aatttgactt tctggaccgg
      301 tcagccgccc gctttttatt ttcagatgaa actcatttaa agaatcattt aattccttca
      361 ctctgcgaga caaagtagcc gaactgatat taagttccat gacaagtttc tgccgatcaa
      421 atgagccatt ttccaacaga taaagaagta tctgatattt tgtagaatag cgcaagtagc
      481 aatgataata aacatcgact gctgctatat ttccatgtct gattaataaa atattccctt
      541 cttggataac aatttccaat tgtttgatat tttccaagtt agcttgtaaa taaaacagat
      601 cttctttcaa ggtagcccga tcaactgata agcgggtaag tacgttatta acggaaatct
      661 ctcctttttc ctctaataaa agttggaaaa gtttgatttg ccgttgttca tttttttcaa
      721 gcaaactgaa aagttccaag tccactcctc caagcatttc tgattttaac agcagaagtt
      781 tccttctcat ccttgaaaaa gaggatctgt gacagttatc acagatccat acaaattcta
      841 atcacttagc tggttggccc agctgtcagt tcccatgtga ccatgccttg atacgttgcg
      901 ccaggttgag cattcgtact gctatttacc tctaagagta aaccatcggt atcgggaaag
      961 tcaacgagaa cctctccttt agctgttcca tcactctcat aaatcaacgt gttttgacct
     1021 tgctgaatca attgttttga tccagaatga ttgaagtata attttgttcc gcttaattct
     1081 tgaccttgat cattcttaaa taaagaagtc aatgtccctg tcagttgcca aggagacgtg
     1141 cttggaagcc gagtatcccg cacatcaaaa gaaaaatcgc cttttgcttc ataataactt
     1201 gtcttcaacg gtttggggag atcgttacca aatgtaaaat catctggtac attaactaat
     1261 gttaattctt gtttggggac aagaattgtt ttagcagatg tttggacaac tgggttagga
     1321 agcttagcac catctgctaa tgtaatattg ctggaaagta cagctgcctt tgtcagtatg
     1381 ctgacggatt gtacagaagt atctacagta tttaattgat agttgtaagt aagatgaatg
     1441 gtatctccta ttttgctcaa tagatctgct gggacagaaa ctccttgttg aatgccggta
     1501 tctgcgatag ttgtaccatt taattttaag gaaccattga tataagtagt attttcagga
     1561 agtgagatcg tgattttttt gtcggttatc gcatccccag attttgttaa cgtgaatgtc
     1621 acattttctt gaatcgtatc tcctgataga gcagacaggg gatctcctgt ctcaggttgt
     1681 ctgctaaggt tttttagctg actatctgca ctcacagatg gaaaattagc cactgttatt
     1741 tgaggaagag tggtgattcc agcttgttca gactgccatt tgtacgcaaa agacggatac
     1801 atgttagtaa gtacatcgtc caccaagcga taatcaaaat aatattgccc tgaagccaat
     1861 gcaggtagtg tagaagtagc tgtatttaca cccccgttat ttgattggat cgtttggaaa
     1921 agttgatctg tccatcctgt cgctaatttt tgggttcctt tcgaataaag cctgtaatgg
     1981 atcgaaccct tttttagttc agttgctgac caattggccg tgacttggta cgatgcagaa
     2041 gaaaggagct ttccccactg actatcggct gttaagtttg ttggtaaaac cgttccgtct
     2101 agcttgtact gctccaatac ctttaaagca acaggagaaa gcaatccgcc ttgcgcatct
     2161 ttcccactgt acacgatatt ggctgtctga ttcaatccac ttgtgcttgc tggagtatga
     2221 gtcagtattg ctccttgtgt aacgtttttt cctgtaaaat agttgactac gcgatcaaag
     2281 cgactaaatg gcaactgatc aaaacgtcct tcagtcagcc ccatcgtcaa tgtccgacta
     2341 ttggtagagc ctggaaggtt ctggttattt ccatccacgt attctgtggt atcagcaaat
     2401 gtattatatt ttcctgttga atcacttgtt gagatgtaac tattccacgt atttatctct
     2461 gcacttgcag aaaatgcata tgctcctgga ttcaagtttc cgccagttaa tttaggataa
     2521 aatctacctg cagctggagc taaaatcgaa ttgaacatac gatcttggat aatattattc
     2581 gaatcataaa aatcaacaga aaaaacggcc tttttagaca tagaaggttg taatttaccc
     2641 ttggtcaaaa ttttgtttgg actcgtatca tatttatctc cgttttctgt catcttcgcg
     2701 acattagctg taacatcaaa ttgaagcggg atatcgtcca cgttgaattc gcctcgcgta
     2761 ataatagcaa taatatctgc caatagagta tttgtatccc acgaccccat cccaaaacgc
     2821 aaggtatggc ttcctgctga taataaacta ccgctagtaa ccttgatggg ggcaccggca
     2881 atatacaagt aggcgttgtt ccagtcaatt gcttttaata catcactgct tgatacatgt
     2941 tccggcaaat tgatgatcgc cacaaatgca aatccttgtg cagtgatatt caccaaatca
     3001 agtacatttg ttagtttcac atggctttcc caatgaaaac ggaccgtgcc acctttccaa
     3061 ggatcatttg aaccatcgtt caaataagta gcagtcaatt taggcaattg gttagcccca
     3121 gttagttgga caagcttccc gccattgact gcactacgtc caccatttgg aggtacgata
     3181 ttttcatacg tgtatggatc ataagaaatg gcaggaggcg ttgctccaac agcttctgcc
     3241 gaaggtgaaa agataaatat acttaaaaca aaaagcgaag taaaaaagaa ggagcgaaaa
     3301 aatgaaaatt tattttgtag caacatgttc atcctccttt tcctttcttc tccataacaa
     3361 caaccacgca agaagccata attctatacc aatgatcaaa taaagaaaac ggatcttttc
     3421 ttcattcgtc tccgggaata cgccatgttg attagattct ttataaatcg gtagctgtgt
     3481 ctgctgtttt gatgcgccat tgactgaacc acttggatgc ggaactggac ttagatcata
     3541 aaatgtaatc gtacctgaac ttttggtatc tgcttggact ggctcttgcc agctgaagaa
     3601 cagcaaactt gcaataatcc ctcccaataa gatcgatgtt ttcaacgaaa tcacctcatc
     3661 atgttgtttc tccttttttc tttttcttgt ggacccacaa aaacagcaaa atcaaccata
     3721 acactagaac tgcagcaacc aatcctgcat aaagccatgg gaaagattgt tcttgtactg
     3781 caaaaacgga attttcattt actttttgtg ctttttcttt tttgatcgta aagttttgtg
     3841 taaatcgcca ttcattactg tcgcctgatt ttgctatgat ttctgcgcgg taatcgccgg
     3901 cttttgcttt atcaccagct aaatcaagat tcaagtgaaa attcgaattt ggtgcaacta
     3961 aaaattcatt agacgtattt tcaatataaa gctgattttc ataaaagatc ttgctcgtaa
     4021 atatcaaatt gctgataatc ctaggctgag gattttggaa acgaagctga acagttggct
     4081 ttgcgttgac ctcgtccaaa tctcctgata aaagtttcag atccggttct atcgtctctt
     4141 gactttcttg taaaacgact gcgagattat aagcaatacg atttttgatt tgtgctcctt
     4201 ctgtagcatt ttctgtttca gcttcttctt taggtgaaac atgaataccc gctaatacac
     4261 gtcccttaaa gctttttgct gggattttca cttctatagg tatagttgtt tctgcatgtg
     4321 ctgatacatt gatttccttt ttcgcaatga gcaaaacgtc acggacatca aacggcaatg
     4381 tctcatctaa cgttgggttt ttttgactat aatcgatcgt tccaccatca ctcgttaccg
     4441 ccggattgac agagacctca aatgttcgtt cttcatctga agaattgctg ataattactt
     4501 ctagtgtctg attttgttcc ggtgccacta ataaatcaaa ataaccgatt gctttatcta
     4561 tttggttctc tggaaatacc ggtttgattc caaagtctcc tgctgaatcc tttgcttggc
     4621 tgattgacgg aaaaatgaaa aatagactta aaatagaaaa aaacgaaaca aacagtcgtt
     4681 tactttgatt cattgtccac cacatccttt caacaacaac tgcggtaaaa aatccttcac
     4741 ctatagtatt ttcaccgcag tttgttgaat ttatttcata ctctatgcta ctgggccagc
     4801 cacgaggttc caagtcaaat cagccgtgaa tgttccagct gctgctttcg gcactttcag
     4861 tgtgatattt gctggatcat aaacagaaag ccatgttccc agaccttgat cttttgctgc
     4921 tgtaaatatt ggtgtcgctg cacttgcttt actcaaccca gttacagttt gactagttgg
     4981 tccagtggat tcgttatctg acgtactttt aacagtggat gttccaaaat ctaattcagc
     5041 gccttgcaaa acttgtgaac cgtttacaaa atctgaaacc gaaactgtca cgttccaccc
     5101 ttgtgcttgg ccatctgcac ctacacctcg acgatctgat acttggagat aaggtgtatc
     5161 gttttttgaa gcgtctactt gatacgtttt tgttccgctt tcaatctcgt gtgtaccaaa
     5221 tggcaattca ggaactacat ccaatgttaa agaacctgtc tggccagttg ctggattttc
     5281 cggatctgct ggctctactg gttttgaagg atcagtaggg ttaagaggct caacggtatt
     5341 tgttggttca gtaaaggtaa tatctccttt tgaagtcgct tgaccctcag caaaagctgg
     5401 tgttgccaag ccgcctagaa gaagtgttga aagtaataat gtcccaatca tgttcttttt
     5461 cataataaat cactcctttt tcttagtgac ctaagtatag tagagacagt atgcaaaaaa
     5521 tcctcatgaa ttcatccgct tacttgaaat tatgaccctt tttgtataaa agagaaaaaa
     5581 acaataaaat cgtttacatg aatattcttc cttttttatt aagtatgaaa aaatatttca
     5641 attttatttt ttcgtttcaa attattctat tagtgacatc cttgaactat aaaaatagga
     5701 gtacggcatc atctgccgca ctcctatttt tttcgtcatt tatttcttaa ataaaacgat
     5761 tctcacgcaa ttttattttt ttgatatctt ctactgtttg tgttccagct aattgcatga
     5821 ccatttccaa ttctgtttta aagaaatcaa acacttgatg gactcctgta cttcctccta
     5881 aagacaatcc ataaataacg ggtctgccaa tagcaacaag atcggcacct gatgcgattg
     5941 ctttgaaaac atgttgtcca cgtcgtacac cgctgtcaaa cacgattggt acacgtttat
     6001 ctactgcttc tgccacatat tgcaacgaat caaacgcagc aggcccccca tctaattgac
     6061 ggccaccatg gttagatacc caaattccac cagcaccaga ttctaatgat cgatagacat
     6121 cttcttctga ttgaacacct ttcacgtaga ctggcaaatc agaatgagca gcgataaatt
     6181 ctacatcttt tggactaagt ttttgtttgg aagatttata tacagcatcc atcgtttggc
     6241 ctactcctga ttgataagcc tgaacgattg gcattggcag cgggaaggta aacccgtttc
     6301 tgcggtctgt ttcacggttt ccgccaactg ttgcatccgc tgtcaatacg attgcttttg
     6361 ctccattacg cttggccact tcaagaatat ccaaattgat tccattatct ttgctcatat
     6421 aaaattgaaa ccattgcggt gctttctccc cgcctgcttc tcttatctct tctaaagtac
     6481 aagaagcgta agaacttgct gtatagatcg tcccaaaatc tgctacacct tttgcagaag
     6541 ctttctccgc cttcacatga gctaatccat gagcagcaac tggtgccatg atgattggtg
     6601 cagtcagcat ttcttcgtca aaatgtgtag ttgtatcggg taattctaca tctcttaata
     6661 catgaggaat aatcaaacga tgattgaatg cccgttcatt ttcttgataa gtaaataagt
     6721 cgccagcacc actgctgatg taaccataac caccagctgg aatcacttgt gaagccgcga
     6781 tttctaaatc ttccatattg ataaaatcta ctattccttc atttgtacct gcttgatatg
     6841 ttttttccat ctcaatgcct tctctctata aaatttgaat gatgaacaat atgatagcgt
     6901 ttacactttt tatattactc ttcgacattg atacaggcaa ataatctgtt tacatttcag
     6961 ccattccaga tgtcactact ctggaagaaa tgattgtaat aaaaatagat aaaaaaccga
     7021 tgggactttg acaaaagtag tgtcaaagtc cctttttttg aataaacggt gttcaaaagc
     7081 cattttctcg gtattaagtc aaatttatgc aaaaatgtgg aaagccattt tcacaaaatt
     7141 tttcttacta ctcgaaacta agcgactttt tcacaacctc tcgataaaac ggtgttcaat
     7201 cagctgaccc tcggtattag ctcaaaaaat ggcaaaatat gaggaactat tttgccattt
     7261 gtctctctaa tactcaggtc agaacgttga cttcacagtc tcttccttaa acggtgttca
     7321 aaaacttgca tctgcggtaa taagcccaac caaacgaaaa ataggaggag ctatttttgt
     7381 ttggctgttc ttattacttg atgcagggca ggtttttcac gacctctgat tcacggtgtt
     7441 tctttttcat caacattttt acgaaatact gtggaagact attgaaataa gtgtggattg
     7501 tttctttgtg tttcaatcgt ttcttgccat tcttcattta attttggatt accgttttct
     7561 tccttgaact cataaggtcc taattcaaaa atcccagaca gtatttgatt cacgatttca
     7621 tatagttcag tgtgatcaga cacatgattc ttgaagatca tctcattatc accacgtttg
     7681 aacctcacat tcgggctctc atgatgaacc tctatgcatt tttctccttt ttttaactga
     7741 cggacttctg aaaacatctc ataaatatct tctccagtaa aaccttcagc aagcgccaga
     7801 ctgtccacaa gaccgcctac tgtcagttca gagattattg cttgattgga atctaatttt
     7861 aatgtatagc ttgtcgcttc tactacgcca catgcattgc aaccgccttc tgatagttcg
     7921 ctttttctaa tcaattgaac ttctttcatt ttctcttcct cctcttaatt ttctacttat
     7981 gtatttttat cacctgacat aggtgactcc tatcaacgct atcatgtgct tttttcctca
     8041 gaaatcctat ttatccattc catctatccc ttattcttct acttttcttt ctctagcata
     8101 taagagatac gcatagtatt caagcaagga ttgtcgatat aaaaagaaca aatactgcta
     8161 ttttgatttc ttttggctat attacatata aataaaattt atcatacctg ctctttttga
     8221 ctatatcatc atctgacttc tatacttcgt ttgtattaaa acttaggagg aatcattcat
     8281 gcttacaggg ttaaacttat taaataatcc ttttctaaat aaaggaacag cttttacaaa
     8341 agaagaacgg gcaaaatatg gaatcactgg tatgcttcca agtactgtcc aaacactgga
     8401 acaacaatct gttcaagctt atggtcaata cttaagcaaa caaacagatt tggaaaaacg
     8461 tatcttctta atgaatctat tcaatacgaa tcggacattg ttttataaat tgatgggcga
     8521 acatttagtt gaattcatgc ctgtcgttta tgatcctgtc gttgcggatt caatcgaaca
     8581 atacaacgaa attttcttga aaccgcaaga tgctgcattt ctttcaatcg acgaaccaga
     8641 aaacatcaaa gcaagtttga agaacgcggc agatggacga gacattcgtt tgattgttgt
     8701 gactgatgca gaaggaatct taggtatggg cgactggggt gtgaacggtg ttgacatcgc
     8761 tatcggcaaa ttgatggtct atactgctgc tgctggtatt aatccagcac aagttttgcc
     8821 tgtatcgatc gacgctggta ctaataatga agaattattg aataatccct tatacttagg
     8881 caatcgtcat gctcgtgtag aaggcgaaac ttactatgaa ttcatcgatc aattcgttca
     8941 atcagctacc gaattattcc cagaattgtt attgcattgg gaagattttg gacgaggaaa
     9001 tgctgcagcg atcttagaaa aatacgaaga tcaaatcact actttcaacg acgatatcca
     9061 aggtacagga attgttgtcc tagcaggtgt attaggcgga ttgaatatat ccggtgaaca
     9121 attgaaagat caaataattt tgacatttgg tgctggtact gctggtgtgg gtatcgcaaa
     9181 catcctttta gatgagatga tccgtcaagg tgtacctgaa gaagaagcac gccaacattt
     9241 ctatcaagta gataaacaag gtttattgtt tgaagataca gaaggattga ctcctggtca
     9301 aatcccattc gctcgcaaac gctcagattt cactaatgct gatgaattga caaacttaga
     9361 agcagttgta aaagcgatcc atccgacaat catgattggt acttctaccc aacctggagc
     9421 attcactgaa gaaatcgtga aagaaatggc tgctcataca ccacgtccga tcatcatgcc
     9481 tttgtctaat ccaacaaaat tggcagaagc aaaagcaaaa gatttaatag aatggacgga
     9541 cggaaaagca ttagttggaa caggtatccc tgctgctgat gttgaatata atggtgtaac
     9601 ttaccaaatc ggtcaagcaa ataatgcttt gatgtaccca ggtttaggac ttggtttgat
     9661 tgcttcaaca gctacacgag taaatgctga aatcatttct caagctagtc gagcattagg
     9721 cggaatcgtt gatgtaacaa aaccaggagc agctatcttg cctccagtag caaaaatcac
     9781 tgaattttct caaacgattg ctgaaactgt cgcaaaatca gttgtcgcac aaaacttgaa
     9841 tcgtgaagaa atcacggata tcaaagaagc agttgaatct gctaaatggg ttcctgaata
     9901 taaatcttta gaagattaag atcgttatag taaaagaaca tacagaaact ttgtatgacg
     9961 tctaaaaaga aaaaagcgac tgcggcaaaa cagcggccag ccaactcctg ctcttttgtc
    10021 gcttttcttt ggcttttttc atatgataca gaataagaaa ggatctgtaa aaatggcaca
    10081 aaaaacaaca actgcaactg cagttgagga aaagaaaaaa gaaggttttt ggtccacaaa
    10141 aatcaccggc gtaagtttgc cactttattt gatcatggtc cttgtcctga tcattgcaat
    10201 ggctttaggg aaattaccga cgaatatgat tggaccaatc gcagttttgg ttattttcgg
    10261 taatttattt cattttatcg ggacaaaaat ccctatcgtc aaaagttatt tgggtggtgg
    10321 ttccgttttt gcgatcttcg cttctgctgc gattgctaca tttgggatct tacctgaata
    10381 tgtagtgaaa tccacagaaa actttgtcag caatatgggc tttttggact tttatattgc
    10441 tgccttgatt actggtagca tccttggtat gaaccgtaat ctattgatga aagcttctgt
    10501 tcgttttatt cccgttgcac ttatttcaat ggtcgtctcc ttttttgccg taggactcgt
    10561 gggcatgctt atcggaaatg gttttgcaaa ttccgtttta tacatctcat tacctgcgat
    10621 ggctggtggt gttggagccg gagccgttcc tttatcaacg atttatgcaa atgttttagg
    10681 tacggatgct tcttctatca tttcccgcct gattcctgcg acagcaatga caaatgtctt
    10741 agctatcatc ggtgcagcat tagttgctcg tgctggacaa tcaatgccaa aattcaacgg
    10801 caatggtaaa ttaatggaaa aaggcgatct tggcgatgga aaacctcgaa ctgtgaaacc
    10861 ttccattcca cagttaggtg ttggtttgat ggtatcattg actttcttca tcttaggaca
    10921 aattggtaac tattttgttc ctaaagtcca tgcttacgca tttatgatta ttatcgttgt
    10981 catctgtaaa atcgccaacc tattaccaga gtattatgaa gatgcagcta tcatgttcaa
    11041 taacttgatt gtcaaaaact tgacagcagc tgttcttgcc ggtatcggta ttgcgttgct
    11101 gaatctgaat gtattagctt ctgctttgac atggcaattt gttgtcttat gtctgacaag
    11161 tgttatcgcg atttcagttg tttcaggatt tgtcggtcgt ttgtttggtt tatatccaat
    11221 tgaatcaact attactgctg gcttatgcaa caactcgatg ggcggaacag gaaatgtagc
    11281 cgtcttatct gcagctaatc ggatggaact tattgctttt gctcaaatgg gcaatcgact
    11341 tggtggtgcc atcgttttga tcatctctgg tttcttgatg caattacttt cttagtcaaa
    11401 aaaacaaaaa tacctgatca tcaagtatcg gcttggtgat cgggtatttt tttgataaag
    11461 atttacagga ttttcaaaaa tcttttttgc attttatcgg aatgataacc gatgtgttca
    11521 taaaattggt gtgcttctac tctgtattct gcagaattaa gccttattgt cgttattcct
    11581 cgttttttcg cttcttctac gccagtcatc aaagcctttc cgatgcctct tttttcagca
    11641 tatttagaca cagcaagtgc cataatattg aacattggat cgctaaaaat cgattcatag
    11701 gactccgctt gtacatagcc agctgctttt ttagaagtag gatcttcata aaccaagaaa
    11761 aaatttttct ctggctcctt cagcagtttc ttcatttgtc tcaccgtaac ggttaatggt
    11821 atatcatacc ctaattgatc cgcattgatt tcacgtagtg cttctgcatc ttctaaacgc
    11881 atttcccgta acatccaaat caccagccaa tttctgattt tttaaaatga atttttttat
    11941 catagtatac tctttttttc atttagaaca atatgtttca gtgattttga cttgcgttag
    12001 tctagattct cctaatgttt gtaagatgac aaaggcattg taacaattga ccaaaagaac
    12061 cacgaggaca attacttgaa aaataaaggt cacttttttt ggagacaaaa ccctgctaaa
    12121 tcttgctccc cacaaacctc caataacagc agcggggata acataaaaca acatagataa
    12181 atcaaagcgc ataaagctac ttgttaaagc aatcgttacc aattttgcca gttgagaaaa
    12241 gaaaatcgta gatagagaat atagtgttgc ttcttttatt ggcaatgcaa acatcagcat
    12301 caacaaagaa acattgactg gtccaccacc aatacctaaa aaactagcta aaaagcccag
    12361 aaccccccca catataaggt accaagtcca tgacgtcaaa tgaaattttg gctgatgatg
    12421 cttggtgtag aagaacgcaa aaaccaaggt caataccgtc aaaaaaattt ggatcagctg
    12481 cacatgcttc tcgttttcaa ataactgtaa aaaaacttcg aatacaatgt tgccgactac
    12541 tcctccaaca acagctccac tagataccca accgacaatc tgccaattca gtgatttccc
    12601 gcttgatact tgtcgcacag tcgaaacaat ggacatcgtg aataccgcaa ctgttgagta
    12661 aaaagaaatc cctgcaacag aatgggcgcc gataagatca agaatcggtt tgatcaacac
    12721 gcctccaccc atcccggaaa tagcgccaac tgtgttcgcc agaacgatca ccacaaaata
    12781 aatcagacca atcatcttgt atttccttt
//
LOCUS       DACEXQ010000060        12646 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.060, whole genome shotgun sequence.
ACCESSION   DACEXQ010000060 DACEXQ010000000
VERSION     DACEXQ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.060"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..599
                     /locus_tag="IXH04_001216"
     CDS             <1..599
                     /locus_tag="IXH04_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1851836.1"
                     /translation="ALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHV
                     GNEELSLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFV
                     QENYEKEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLT
                     LTEICEKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            596..2332
                     /locus_tag="IXH04_001217"
     CDS             596..2332
                     /locus_tag="IXH04_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ1851837.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(2395..3231)
                     /gene="pheA"
                     /locus_tag="IXH04_001218"
     CDS             complement(2395..3231)
                     /gene="pheA"
                     /locus_tag="IXH04_001218"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAQ1851838.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(3228..3737)
                     /locus_tag="IXH04_001219"
     CDS             complement(3228..3737)
                     /locus_tag="IXH04_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAQ1851839.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(3730..5025)
                     /gene="aroA"
                     /locus_tag="IXH04_001220"
     CDS             complement(3730..5025)
                     /gene="aroA"
                     /locus_tag="IXH04_001220"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAQ1851840.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(5038..6129)
                     /locus_tag="IXH04_001221"
     CDS             complement(5038..6129)
                     /locus_tag="IXH04_001221"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAQ1851841.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(6146..7312)
                     /gene="aroC"
                     /locus_tag="IXH04_001222"
     CDS             complement(6146..7312)
                     /gene="aroC"
                     /locus_tag="IXH04_001222"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAQ1851842.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(7314..8378)
                     /gene="aroB"
                     /locus_tag="IXH04_001223"
     CDS             complement(7314..8378)
                     /gene="aroB"
                     /locus_tag="IXH04_001223"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAQ1851843.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(8411..9433)
                     /gene="aroF"
                     /locus_tag="IXH04_001224"
     CDS             complement(8411..9433)
                     /gene="aroF"
                     /locus_tag="IXH04_001224"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAQ1851844.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(10092..11066)
                     /locus_tag="IXH04_001225"
     CDS             complement(10092..11066)
                     /locus_tag="IXH04_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAQ1851845.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(11047..>12646)
                     /locus_tag="IXH04_001226"
     CDS             complement(11047..>12646)
                     /locus_tag="IXH04_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HAQ1851846.1"
                     /translation="MYPWEGHVFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLE
                     EGLKGKQISIHFAGVEQAMFIWLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYK
                     RSTAAFLEDQDFFRFFGIFRDVFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQ
                     VAKNSQLNVAILDPDGKEIYQQQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETI
                     DEEKRVTEYIDYSFGFRKIEIKDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEK
                     DLAIIKNAKINGVRTSHYPNQITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYN
                     VPGAVPQWQAAVLDRAKTNFETLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDR
                     LVHYEGNFPNPAFEDSISDVKSCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGG
                     MDSYMTLLDRYEMYQGGYIWDFKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNG
                     IVFADGQEKPAMQEVRYYYEKYSK"
BASE COUNT         3438 a         2818 c         2313 g         4077 t
ORIGIN      
        1 acgctttagc tgtcaaagaa cttgagaatt tcttcacgac gatattggaa cagcctattg
       61 atccagctta tttgaaacat gaggcaatca atcgtttcta tgcattactc aacgctttgg
      121 ctgacgagca tgttgggaat gaagaacttt ctttgctcaa gatcgccctt ccttctttat
      181 tagccgatgc tgagtatttc gaagattttt ctattcttgt caaagaaaca cttgagaaac
      241 tcacccactt ttctgcaaat gcaaaaaaag acaatttcga tatcttgact ttgatctacg
      301 agttcgtcca ggaaaattat gagaaagaaa tcaatttaca acttttatcc gaaaaatatc
      361 atttttcata tagctatctc tcagccatct ttactgaaaa atttggtatc aacttttcaa
      421 aatatctaaa aaaagtccgt atcagtaaag ccaagctctt tttgacggaa acacggctta
      481 ctttgacgga aatctgtgag aaagtcggct ataccgaact gggttatttc tccagagttt
      541 ttaaagaaga gacaggaatg acaccttctc aataccgtaa aggaactttt gtaaaatgag
      601 aaaaaaaatc aaagagcatg aactctttgc caaacttctg ttcattttgt tcatcggtct
      661 ttttctgcaa gccatcgtca tttctttgtt tatttatcat cgatcaaggg atgcctatat
      721 ccagctgttc aatcaatcga atgatgtggt actaaaaaag attcaatctg aatttgaaag
      781 tttgaatgat acaatcgaga atacattagc cgcttttgat tctaatccag ctgtcaaaag
      841 ctatttttcc aatgatccag cccaacatat ggaacagctt cagcaactaa gaacaatcca
      901 aaaaatgaat gactctcttt caaaaatcca tccaatgatc gattacgatg tattgatctt
      961 tggagaaaat ggaagaactt ttgtcggaaa cgacatgctc actgccgtat ctgccgattc
     1021 tttttttcaa tctgcgattg ctcagagagt gaatgaacgt gctgctgata cacaaatgct
     1081 ttttgcacac cacggattga ctcttcgcga taagaaagca cccagcgtct tctttgtcaa
     1141 aaaactgaaa aatacattga atcacgttta cggatatgct gttttatcga tctcatccaa
     1201 gcaactagcg aatctttttc aatctgtagt aaatcctgag atatcgaaga tctccttcat
     1261 gaacgaagaa cagacgatca tcgcatcaaa tgaagaaaat acaattggga gccgatcaat
     1321 gattttcaaa aaactaacag taggtgaaac aacgatttca aatggacagc agttgacccg
     1381 tcttcctctg taccgccagg attgtacatt actcagtcaa gtcgatcttc attctttatc
     1441 taaccaaatg ggcgtgattt ttccgattat tttgtataat atcgtgacac ttgcttttgc
     1501 tggctcattg gtatttatgt atttggatcg acacacaaaa tcgatctacc gcttgattga
     1561 ttcgctaaaa cggctagagg aagatggctt aaaagctact gttcctgtac aaggaagcca
     1621 tgaagtccaa ttgctaggga atacgatcaa tcaattgcta agtaatgctc agcatcatta
     1681 tgaacagacg atccaagacg aaaagaagaa acgttcgctt gaaatccaaa ctatgcaagc
     1741 acagatacag ccccatttta tttataatac gctgactagc ttgaaatttt tgatttggca
     1801 gaaaaaaaca gatgaagcag ttcaaggaat cgacagtttc attactttac taagaagtac
     1861 gattgggaaa aaagaagaag tgattccagt caaggaagaa atcaaaagcg tacaaagtta
     1921 catcgatctt ttgagtatgc gatatggcga aagaatctcg gctaaaataa tgattcctga
     1981 tgaactgcag tcattatcta tgccgaatat gatccttcaa ccaattatcg aaaacgccta
     2041 tcttcatgct tttcacaata aaacgcaagg attcatcacg gtgtacggca aaacaaaaaa
     2101 tcagcttctt ttattcgaag tgattgataa cggcgatggc ttcgatactg aaaataccaa
     2161 acaaaaaaat gatcattttt caggaatcgg catcagccat gtagatgagc ggatacgcct
     2221 aatgtatggc gatgcatatg gcttaacgat ccaatcagtg attgggacag gaacgactgt
     2281 aacgatttgt ctaccgcttc ttcctaaaaa tcaatcagaa tatcaaaaat aaagaatagc
     2341 caagcgctga tttttttatt ttaagttaaa taaatcaatc agtattcttg actttcatct
     2401 acgcgtctct tctactgtca gcactggata acagcccaat atttgacttt ctcctccgag
     2461 catcttgatt tcttggagcg cattctctac taatgtcatt ggacggtcca acaacaaatc
     2521 gatcacgaaa aaatattctc ccaagctagt tttcaatggt cgggattcaa tcttacttaa
     2581 gttgatttcc cgccagccaa aagctgccag cattttatga agcatccctg gccgattagc
     2641 aggtagtgtc aagatgacac tcatttttac tggagctgac tgctggctgg tcatctttcg
     2701 gtcaccgacg atccaaaaac gtgtctggtt caactcatta tcttggatat tctcagctaa
     2761 gatctccaat ccaacatgct gtgctgtttc taatgaagca atcgctgctg cgtcttcttt
     2821 cggatgttct gctacataca ttgctgcagc agttgttgat tctgttgcta ctaaaggaac
     2881 attaggataa tgcgtttcta aaaactgttg tgattgtgct agtgcttgtg gatgtgaaag
     2941 gatttttgta atctttgttg ctggattgcc taaaagctgc tgcttgatag gaagaacgat
     3001 ttcactttta acttcgactt ctggatgttt ggaaagaaga tcgattgtgt gatgaacagc
     3061 tccttccaat gaattctcta caggaacgac agccagatcg acttgtttgc gaaacaaagc
     3121 gcgcaaacaa acagggatgg aagcatacgc agtcaattgt tctgtaggaa atagctgaca
     3181 agccgcctga taagtgaatg aactttctgg ccctagatag ctgacgttca tatgcttttg
     3241 atcctttcta taatggtatc gatgatcttt tctggtgttt gattggtcgt gttgattcga
     3301 tgtgtcgcac tttcttcata ccaagaaagt cgttgtgcca ataatgcttt tatttcttct
     3361 cttgtttttc caagagctaa aggtcgaata gacgtttcat cttgttggat acgatcaact
     3421 agacagtccg cttctgcctc taagtaaaga acaatcggct gtgtttttaa aaaacgccga
     3481 ttttctgcac tttggacaat ccctccacca gtcgcgataa ttttgtcttc atttgacagc
     3541 tttcttaaaa tatctgactc cacttttcga aagctttttt caccgtaata gtgaaaatat
     3601 tcggcaatcg acaattggat atgttcctca atctttgtat ctaaatctac atactctttt
     3661 tgtaaacatt gagctaaccc tttaccgatt gttgtttttc cagcgcccat gaaaccaatc
     3721 agcaaaatgc tagccatgag cttctcctcc tgacacaagt tctgctaaat cgtcaaaaaa
     3781 tgccggataa gagattgaaa cagcttcagc tccttctagt tcgcaaggtc catcagctat
     3841 caaagcagct acttgcagca tcataccgat tcgatgatcg cctctgctgg acacttttgc
     3901 gccatgcaga ggtgtcggcc catggatgat cagaccgtct gctgtcgctt ttatctttgc
     3961 tcccattttc tgtaattctt ctgccaccgc atcaattctg ttggtttctt ttacttttaa
     4021 ttcttctgcg tctttgatga tcgtgattcc ttctgcttga gtagccacta aagctagaat
     4081 gggcaattca tcaatcagac gcggaatgat ttctccacca atcgttgctt tcttcaaatg
     4141 actcgtcttg acacttaaat cagccgattg gttctgttca ttgtgattcg tctgggtaat
     4201 cgctgcgccc atctcctcca acacatctaa gataccagtt cttgttggat tgaccccgac
     4261 attttttaac aacagttggc tttccggaac aagaagtcct gctgctagaa agaaagcagc
     4321 tgatgaaata tcacctggaa cagttatctg ttgaccagtt aatttttgcg gtccggtcac
     4381 tatgatcgtt ttatcttcca ctgtgatcct tccgccaaat tgacgaatca tttcttccgt
     4441 atggttacgg gaacgttctt tttcaacgat tacggatgtg ccttctgctt gtaatgccgc
     4501 aaacagcaaa gcagatttga cttgcgcact ggctacaggc atattgtatc ggataggtga
     4561 taaagagcgg gttcctgtca cagtgattgg tagatactgg tcattttctt ctccttgcaa
     4621 gtcagcgccc atctcgcgta aaggttccat cactcgcccc attggacgtt tagacaacga
     4681 agcatccccg aacagagtag tcgtaaaagg ctgtccagcc aaaataccca tcaataaacg
     4741 tatggtcgta ccggagtttc ccatgtcaat tgattcggtt ggttggacaa atgagtggct
     4801 accgcgacca tgaatcttga tcacctcttc ctcttcttcg atctcgacac ctaattgttg
     4861 gaaggcatgt aaagtactca gacaatcttc tgcgcgcaaa aaattttgta tagtcgtcgt
     4921 tccttcagca attgctccaa acatgatgct gcgatgggaa attgatttat ctgctggtat
     4981 cctaacagtc cctcttaatc catgtatttg ttgtaataat tgcatttatc cagcctccta
     5041 tttgatccag caatgataac tggtattaga cataatgatt ctttttgctt gtaacaaatc
     5101 ctgttggttt ttaaaactga tttgcagtat tcctaaaata tcttccctgg tctcttggat
     5161 cttcaaatta atcaaagaaa ctcccgcttt tccgatcagt ccagttactt ctgcaataac
     5221 gccaggttca tctggcacat cgataaataa atcaaaaaag gcaggaatcg cgccttgctt
     5281 atggacaggc aactggtctc gactttcttt tgcccgattg aaaaaagaaa agatttccga
     5341 ttggttctca gtcatgatcc attctgctat ttgtgtcatt tcttgttgcc aatctgccaa
     5401 tcgttccaat aatatctgct tattgctcaa caaaatatct gtccacatga caggatctga
     5461 ggaagcgatc ctagtgatat ctcgaaagcc tcctgctgcc agttgtttgg ctcgcggata
     5521 ctcttgattg aattgttctg cctgattgac taagccagct gccagaatat gtggaaaatg
     5581 acttagcatt cctgttatac ggtcatgttc tcttgctgta agagttacat attttgcttt
     5641 ggttccgcaa aatagttctt gcagtttttc aacatctttt ttcgtctttt ccaaaggtgt
     5701 aaagatgtaa taggcatttt caaatagttt agcatctgct gcctgtatac ctgatttatg
     5761 agaaccagcc attggatgtc caccgacaaa ccgaagattt ttctgctcag cggatgtcac
     5821 gacttgctgc ttcgtactgc tgacatccgt aatgatgacg tgatcagaca agttgattct
     5881 ttcaaggaca tcaagataag ctaaggttgt acggacggga acggctaata agataacgtc
     5941 tgcttgttct gcacctgttt caaaatcact tgcaacgtaa tcaacgatct gctgttctac
     6001 tgcttgctca cgggtttttg actgactatc ccaaccaact actatcagtt ctggattggt
     6061 ctgacgaata caaagtgcca tagaagcacc aatcagtcca agccccacga taaaaacatg
     6121 ttgtgccatc ttatttgccc cctttttagt aatgtttcaa gtagtcacgg tactccttca
     6181 ctgcagcttt caactcaaca agcgaatcac ttgggaattt tgacaatagc tctgtcgcta
     6241 ccacagtggc tacaacattt tccgcaacga cgctagctgc aggaactgct gtactatccg
     6301 aacgttctac acttgctttg taaggttctt ttgtatcgat atcaacactt tgaagtggct
     6361 tgtacaatgt aggaatgggt ttcatgaccc cgcgaatgac aagtggttcg ccattggtca
     6421 ttccgccttc gaagccgcct agattattgg tacgtcggaa atagcctctt tcttcttccc
     6481 ataggatctc atccatcact tctgagcctg gtaatgaacc agaagtgaat ccgaccccaa
     6541 attctacgcc tttaaaggca ttgatactaa ccacagcttg tgcgattttg gcatctagct
     6601 tcgtgtccca ttgtacgtag cttcctaatc cagctggaac gcctccaacg agcacttcta
     6661 caattcctcc gatcgtatct ccatttttct ttgtctgatc gatcaaatct tttattcttt
     6721 gctcaactgc tggatcaaca actcgtactt ctgacgcttc agaaatttct ttgatttctt
     6781 gaatcgagat atttccaggt atttctgctt ggattccacc aagacaaatc acgtgtcctg
     6841 ctacgatgat gtcaagttct tccagaattt tcttggcaat tgcgccgatt gctacacgca
     6901 tggtcgtctc tcttgcagag gaccgttcaa gcacattccg aagatctcga tgatggtatt
     6961 tcataccgcc taccaaatcc gcgtgtcctg gacgcggacg tgcaactcgc cgcagtgatt
     7021 ggtctttttc aggcacttct gctacagaca tgacagttgt ccaatttttc caatctttat
     7081 tttctaccac taaagtgaca ggtgaaccaa ttgttttgcc gtgacggatg ccagacgtga
     7141 tccgaacacg atctttttcg atcttcatcc ttcctcctct gccgtaacca gcttgtctgc
     7201 gagcgagttc gtgattgatt tcttcttctg acagaggtaa tcctgctggt agtccttcaa
     7261 taatggctgt taattctggt ccatgggatt ctcccgcagt catataacgc atcttacagt
     7321 cctcctaagt aatctttgaa tgtctctgtg ggaacaggaa cgatttttgc ttgcccaatc
     7381 ttttctagca agatgatatt caattggctt cctcttgctt tcttatcatg ggttatcgcg
     7441 tggaataatt catctggatg acttagatcg tacgtaatcg gcagatggaa tttttgaagt
     7501 atcctttcta aagcagctgt cgatccagtt ggagacaagc cgattttttc tgcatgagaa
     7561 gtgatggcta ccattccaat agctactcct tctccatgac tgactacccc atatcctgct
     7621 gtattttcaa ttgcatggcc aattgtatga ccaaaattta aaataagtcg atttccttgg
     7681 tcaaagacat cttcttccac tacttttcgt ttgatttcca acgtagcagt aatgattgga
     7741 acagcatgtt ccagaagatc tttttcgtct ttcagctcgt tcagctgttt ccaaagcgtt
     7801 gcatctgcta tagctgctga tttgatgatt tcagcgattc cttctcgtaa acgacgtacc
     7861 ggcaaagttt ttagaacctc aggatcaatc aatacaccag ctggctggct aaatgttccg
     7921 acaaggtttt tcgctttgtt tgtattgact gctgtcttcc cgccaatact agaatctact
     7981 tgtgcaagta gcgtagtcgg aatttgaacg aaatggatcc cacgcatgaa cgtcgaggcc
     8041 acgaatgctg ctagatcacc caccattcct ccaccaagtg caagtatccc atcgctgcgg
     8101 gtaaaatcat tatctgctaa aaaatcgtat aactcagctg cttgttctaa agatttactg
     8161 gtctcacccg ccggtacgat tgaaaggact ggttcaaaat tagcttgtct caattggaac
     8221 atgatttttt ctgcatatag tggagcaacg tttgtatcag tgatcacagc tactttttga
     8281 ggtctccaaa tcgtttgaag ccatgatcca gcctgttcta gtgcgccttt ttcaatatga
     8341 atatcgtatt tttggttatc aacatgtaca gtaagcattc gtttcacttc tttcttttaa
     8401 aaagctggaa ttaatcgagt gtctgaatct tttccattgc ttctttcatg atcgcaactt
     8461 ctttcatcat gcgtgagtaa gtcttttcat tcaacgattg gggaccgtct gaccaagcat
     8521 ttgcaggatc aggatggatc tctacgatca tgccatctgc tccgcttgcc acacccgcac
     8581 gagccattgg tggtacaagt tcccaaattc ctgtaccatg acttggatca acaatcacag
     8641 ggaaatgagt taattttttc atcaatggga cagcacttaa atcaaacgta ttacgtgtcg
     8701 ctgtttcgta agtccgaatc ccacgttcga taaaaatgac aggagatttg tcttcgactg
     8761 caatgtattc tgcagcatta agccattcat tgatcgtacc ggaaattcca cgtttcaatc
     8821 caacaggttt acctgtttta ccgacagcag atagcagttt aaaattttgc atattacgtg
     8881 cgccaatctg taggatatcg ctgtactcag ccaccatatc gatatgccct tcatccatca
     8941 cttcagtaat cactttcatt ccaaattcat cagccgcttg acggatgtat ttcaatcctt
     9001 cttcttctaa accttggaaa gcatatggag aagtacgtgg tttgaaggca ccacctcgca
     9061 gaatttttgc gcccccggct ttggccattc gcgcacattc tctgatttgg tctaatcctt
     9121 cgatagaaca aggaccagcc atcgtcacga aactgccgtc accaattttt actccgtcta
     9181 catccacgac tgtattgcca ggatggaatt ctcgagaagt caatttgtat gtgtttaaaa
     9241 tacgcacagc attttctact ccttcatagc gtgtaaatgc tccttcttgt aaacttcgcg
     9301 tatcaccttt tagtccaatc actactcgtt ctgttccttg atcgattgag actttgaatc
     9361 cttctttttc gatatgatcg acgacttttt ttatttgttc ttccgttgct tcttttttca
     9421 taattactat cattgttttt cccgctttct tttgtttact tatctatctt ggctgatttt
     9481 ctctactttt tcaaggtggt tttacattaa ttcctactaa atgaccgtgt cctgttgttc
     9541 tcattttcgc tccttctttc ctacttatga cccagataat caacaaaaaa agatgtccct
     9601 gtaggaaatt gcctctacag tgaacatctt caacgagcct taaaaagcgc tgcaaaaata
     9661 taaaatagcc actatagatg aatttctcta cgtggctgta agtcgtgttt aaaaatcttt
     9721 agccatcata gatacaaacc tggtttgttt gtacccacga agtcatgaat acaaacctat
     9781 ctaaaatagc taaagtaata cgtattcagt tttgtaagtt ttctagattt cataaaaaat
     9841 cgctccaaac tgatgtaaga attgtaccta gtatagacat aaaaaaatcg actgtcaatc
     9901 atttttatga aacttttttt aattttctga tagttaactt tgtttttcag aaaaataaca
     9961 attggtttta ttgaagaaaa tagtcatgaa caaaagtatc tgagaacttt tttactctct
    10021 tgttcatgac atatagaata aagcagcttt gtcttgtaaa ctccgttttt caaataataa
    10081 tctaaagatt gctactgaat aataaagccg atttcatggt cctcttcgct gctgattcgg
    10141 taaggtgact caacatccgc tccccagcta ttgatacccc cgactcctct tacttttgca
    10201 aaaatcgtaa gaaccgttcg tctttctggc ggtagttctt ctatatgtgt tgcctgttcc
    10261 agttcttctg ctttgaatgg taaagcggaa aaagcaaatg gcgtatcctg ctgttcaaat
    10321 cgcaacgaaa aatcttcttg atctttatcc gtattatcca acgtttgatt tcttgttagt
    10381 tctacccatt tcgtttccat atgcatgccg cattcttggg gaacaaggta tggggaaacg
    10441 gggagtcctg ccactttata aataccttcg acaccaccag ccatccgatc cggatatgtt
    10501 tcccctgata ggccttcata ctcaaaaaat tttgctttcg ttggcataat gaaccttaaa
    10561 cccaatgcgg gtaattccgg taatcctttt tgtccataat aatgcatatt gacaagcatt
    10621 ttaccctctc cagcgatttg gtaggaaatt gtcacttggg tttttggtac tgtgttgatt
    10681 tccagtgtaa aaatgatttc aaccgtttca gcgtattcat tacttgaata ttgattattt
    10741 actggagcaa tcggcaaatc gatcttttta ttgtccacca gtacttctat ttctttgact
    10801 tttggataca atccggctcc taaccacata ccggatttaa atccaaatcc gctacctctg
    10861 tcattatcgg tcgttgctcg ccaaaaaact ggaagtgttt cccggtatag ccattctttt
    10921 ccgtttttat tgagcgattc cagtccgcct cttgtataag aaaaaatata atgcgtattc
    10981 cccgatttga cacctaatgt acagtcgccg aagacgattg caactttttc cgtatccata
    11041 tgacttttat ttactgtatt tttcataata atatcttacc tcctgcatgg cgggtttttc
    11101 ttgtccatct gcaaaaacga tcccattccc tgagaattca tagtcacttg gtttatcgtc
    11161 aaagtcaccg ccatagcgca tcacttctct gcctgtgatt ggatcagtca cttggatcgc
    11221 ctgatcttta aaatcccata tatagcctcc ttggtacatt tcataacgat caagcaacgt
    11281 catgtaagaa tccattccac ctaacgaatt ccccatgtca tgcatatatt cacataagat
    11341 gaacggtttt tccgctttat tttcaaggta gtcaatgatc tcttctggtg gcgcatacat
    11401 gcagcttttt acatctgaaa tcgaatcttc gaatgctgga tttggaaaat tcccttcata
    11461 atggacaagt cgatcggggt ctactttttt gaagtaatcg ttcatttgtc gaatgttttc
    11521 ttccgcataa gattcatttc caagagacca aaaaagaata cttggatggt tttttaatgt
    11581 ttcgaaattc gtttttgctc gatcaagaac agccgcctgc cactgaggta ctgcacctgg
    11641 tacgttgtaa gaaggctcaa atgcgcccat tttttgccat gagccatggg attcgaggtt
    11701 cgtttccgcc atcacatgga ttcctgcttg gtcgcataaa taataccaag tgatctggtt
    11761 ggggtaatga gatgtccgta cgccgttgat ctttgcattt tttatgatag ccaaatcttt
    11821 ttccatatct tctatagtga tggcgcgtcc ttttttcgga tgccactcat gtcgatttac
    11881 cccgttgata atcatccgtt ttccattgaa gtagatgatt ttatctttga tttcgatttt
    11941 acgaaaaccg aaggaatagt caatatactc cgttactctt ttttcttcgt ctatcgtctc
    12001 caattcaagc ttatataaat tgggctgaaa atgcccccac agtgccacat tttcaaatcc
    12061 atcgatgcga atcgtcattt gctcttttaa cggatattgc tgttgataga tttccttccc
    12121 atcaggatct agtatcgcta cattcagctg actgtttttg gctacttgct caaaagacaa
    12181 atctacttca agtttgccat tttgacattt gtcatctaag cgaggtttga tccaaagatc
    12241 ttctaaatgg ttttgtcctc gagcaactaa gaagacatct ctgaagatcc caaaaaatcg
    12301 gaaaaagtcc tgatcttcca agaatgcagc tgtacttctt ttgtaaacct ccacagctag
    12361 aatattttct tcttccaaat aaggtgttaa atcaaattca gacggagtga acgaatcttc
    12421 ggcatacccg ataaaatgac cattcaacca aataaacatt gcttgttcta caccagcaaa
    12481 atgaatcgag atttgttttc cctttagacc ttcttctaat cgaaaagtcg tcaaatacga
    12541 accaaccgga ttgtctttag cagctgaaaa gatcccttcc gcctctttct cagggcttgt
    12601 cgtgaaaggg ggacggcgaa atacatgtcc ctcccacgga tacatc
//
LOCUS       DACEXQ010000061        12506 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.061, whole genome shotgun sequence.
ACCESSION   DACEXQ010000061 DACEXQ010000000
VERSION     DACEXQ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.061"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..410
                     /gene="uppS"
                     /locus_tag="IXH04_001227"
     CDS             <1..410
                     /gene="uppS"
                     /locus_tag="IXH04_001227"
                     /EC_number="2.5.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="di-trans,poly-cis-decaprenylcistransferase"
                     /protein_id="HAQ1851847.1"
                     /translation="AIEQTKNNTGMVLNFALNYGSRAEIVTAVQEIAEEVAKGEIHAE
                     EIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFLLWQIAYSELYFTKALWPDF
                     DGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            407..1207
                     /locus_tag="IXH04_001228"
     CDS             407..1207
                     /locus_tag="IXH04_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAQ1851848.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1368..2636
                     /gene="rseP"
                     /locus_tag="IXH04_001229"
     CDS             1368..2636
                     /gene="rseP"
                     /locus_tag="IXH04_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAQ1851849.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            2704..4413
                     /locus_tag="IXH04_001230"
     CDS             2704..4413
                     /locus_tag="IXH04_001230"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAQ1851850.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            4625..8977
                     /locus_tag="IXH04_001231"
     CDS             4625..8977
                     /locus_tag="IXH04_001231"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAQ1851851.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9121..9594
                     /gene="rimP"
                     /locus_tag="IXH04_001232"
     CDS             9121..9594
                     /gene="rimP"
                     /locus_tag="IXH04_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAQ1851852.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            9618..10793
                     /gene="nusA"
                     /locus_tag="IXH04_001233"
     CDS             9618..10793
                     /gene="nusA"
                     /locus_tag="IXH04_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAQ1851853.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNSKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            10815..11108
                     /locus_tag="IXH04_001234"
     CDS             10815..11108
                     /locus_tag="IXH04_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAQ1851854.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11105..11416
                     /locus_tag="IXH04_001235"
     CDS             11105..11416
                     /locus_tag="IXH04_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAQ1851855.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11429..>12506
                     /locus_tag="IXH04_001236"
     CDS             11429..>12506
                     /locus_tag="IXH04_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ1851856.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQT"
BASE COUNT         4261 a         2193 c         2775 g         3277 t
ORIGIN      
        1 gtgcaattga gcaaacaaaa aacaatactg gaatggtatt gaattttgct ttgaattatg
       61 gaagtagagc agagatcgtg actgctgtcc aagaaattgc cgaagaagta gcaaaaggag
      121 aaatacacgc agaagaaatc gatgatgaat tgattgctaa gcatttaatg acaggtttct
      181 tgcctaaaga gttacaagat cctgagctga tgatccgtac aagtggagaa gagcgaatta
      241 gtaattttct gttatggcag atcgcatata gtgagctgta ttttacaaaa gcgctttggc
      301 ctgattttga tggtgcgcat cttgaagaag caatcgcctc ttatcaaaat cgagaccgtc
      361 gattcggcgg tgtaaaaaaa acagaagatg agaaagggga tcagtcatga aacaacgtgt
      421 gatcacagca gctgtggcgc tagtcttgtt tattccgatt atatggatcg gaggcatagc
      481 ggttgagctt acagcagcaa tcttagcggt agtaggagtt tacgagctgt tccggatgaa
      541 aggattgaca ctcttaagtt ttgaaggagt tttatccgcc gttggtgcag tgttacttgt
      601 tttgccaaaa gaaagatggt ttttctttct tccagagaat gtaagtacat ttatgttatt
      661 ttatcttata gttatgattc ttttaggaac atcagttatt tctaaaaata cgtatacgat
      721 tgatgaagca ggttttcctg tgcttgtcag cttgtatgtg ggaataggtt tccagaattt
      781 tgtcaatgca cgaacagaaa gcttggctgt tttgatattt ggtctattta ttgtttgggc
      841 aactgatatc ggagcatata tggttggtcg acgttatggt cagcataagt taagtcctga
      901 tatttcgccc aacaaaacga ttgaaggtgc gctcggtggt atcggtagtg ctgttgtagt
      961 cgcatttctt tatttattgc tatacccagc aaaagaacta ttcaatcatg gtatgtttgt
     1021 catgcttctg atgacgatta ttttttcagc agtcggacaa tttggtgatt tggtcgaatc
     1081 tgcaatcaaa cgtcattatg atgtgaaaga ctcgggaaat atcttaccag gccatggtgg
     1141 aatcttagac cgtttcgaca gtttgctgtt tgtatttcct atcatgcatt tatttggcat
     1201 tatttagtaa gtatacctgc gtttctttgt gaatttcata aaagacgcag gtttttattt
     1261 ttcattatat tgatactatt ggtgtctttg gtatactatg gtaaaattat agtgttttat
     1321 cgttgtttta taaaaaacac aaaaaacaag taaaggagaa aaaagctatg aaaacgattc
     1381 tgacatttat catcgttttt ggtatattag tgattgttca tgagtttggt catttcttct
     1441 ttgcgaaacg atcaggaatc ctcgtccgcg aatttgctat cggtatgggt ccgaaaattt
     1501 atgggcatca agcaaaagac gggacaacat atacgcttcg cctattacct attggcggtt
     1561 atgttcgaat ggcaggcaat ggggatgatg aaacagaaat ggcaccagga atgcctcttt
     1621 ctcttttact aaattctgac ggcatcgtag agaagataaa tttaagtaag aaaatccaat
     1681 tgacaaatgc tatcccgatg gaattgagcc gttatgattt agaagatgag ctgactatca
     1741 ctggttatgt gaatggagac gaaacagaag tagtaaccta ccctgttgat cacgatgcga
     1801 cgattattga aaacgacgga acagaaatca gaattgcgcc aaaagacgta cagttccaat
     1861 cggctaaatt gtggcagcgt atgctgacca actttgctgg acccatgaat aactttattt
     1921 tggcaatcgt gttattcatc attctggcat ttatgcaagg tggtgttcaa gtgacgaaca
     1981 ctaatcgcgt aggagagatc atgcctaatg gagctgcggc agaagcaggc ttaaaagaaa
     2041 atgatgaggt agtcagtgtg gacggaaaag aaatccattc atggaatgat ctgacaacag
     2101 tcattacgaa aaatccaggt aaaacattag actttaagat cgaacgagaa ggtcaggtgc
     2161 aatctgtcga cgtgacaccg aaatctgtag agtcaaatgg cgaaaaagtt ggccaacttg
     2221 gtataaaagc cccaatgaat acaggtttta tggataagat cattggaggg acgcgtcaag
     2281 ctttcagcgg ttcattggaa attttcaaag ccttaggatc actatttaca ggtttcagtt
     2341 tagataaatt aggcggacct gtcatgatgt accaattgtc ttcagaagca gcaaatcaag
     2401 gtgtaacaac tgttattagt ttgatggcgc ttctttcaat gaatctcgga atcgtcaatc
     2461 tgcttccgat tcctgcctta gatggcggga aattagtctt gaatattttt gaagggattc
     2521 gcgggaaacc attaagtcaa gaaaaagaag gaatattgac attagctggc tttggatttt
     2581 taatgttgtt gatggtgcta gttacatgga acgatattca acgattcttt ttctagaaaa
     2641 agttgacaaa tagggtataa taacaaacga taactatttt gaggaaataa aggagtatat
     2701 agaatgagac aatcaaaaat gttgattcca acattacgcg aagttccaaa tgatgcagaa
     2761 gtattaagcc atcaaatctt gctaagagct ggatatatcc gccaagtatc agcgggtatt
     2821 tattcatatc tgccattagc aaatcgtgta ttggaaaaac tgaaacgcat tatgcgggaa
     2881 gaatttgaaa aaatcggagc ggtagaaatg ctgatgcctg ccatcttgcc agcagaactt
     2941 tgggaagaat ccgggcgtta cgaaacatat ggaccaaact tgtatcgttt caaagaccgc
     3001 aatgatcgaa aaatgatcct tggaccaact cacgaagaaa cttttactga tttgatgcgt
     3061 aatgaaatca attcatataa gaaattacct ttaaatcttt atcaaatcca acctaaatat
     3121 cgtgacgaaa aacgacctcg cttcggtttg cttcgcggaa gagagtttat catgaaagat
     3181 gcatattctt tccatgcaac ggaagaaagt ttggacgaga catataaaga ttacgaaaaa
     3241 gcctataccg aagtattcaa gcgttgtggc ttagaatttc gctcgatcat cggagacggc
     3301 ggcgcaatgg gcgggaaaga ctcaaaggaa ttcatggcga tctcagaaat cggggaggat
     3361 acgatctgtt attcgactga aagtgattat gctgcaaact tggagatggc tacaagctac
     3421 tatgtatcaa aaaaatcgca tgaaacacag cttgaattgg agaaagtagc tacacctaac
     3481 gtaaaatcaa ttgacgaagt agcagatttc ttttctgttg aacctcaaaa aatcatcaag
     3541 tctgtattgt tcattgcgga tgaacaacct gtccttgttt tggtacgtgg agaccatgaa
     3601 gtcaatgacg taaaactgaa aaacttttta tctgctgact ttttagaaga agcaacagaa
     3661 gaagaagcac aaaaatatct aggtgctgag tttggttctg tcggaccagt cggtgtttca
     3721 gatgacgtaa aagtatatgc agaccgttat gtccaagatt tagcgaatgc cattactgga
     3781 gcgaacgaaa caggattcca ctataaaaat gtgaatccag aacgcgattt cactgtgctt
     3841 tcttatgaag atcttcgttt tgtacaagaa ggagatcctt caccagacaa caacggtgtt
     3901 cttgccttta ctcgaggaat cgaaatcgga catatattca aaatcggcac tcgttacagt
     3961 gaatctatgg gagcaactgt actagacgaa aatggacgag aaaaatttgt gattatgggc
     4021 tgttatggta tcggtgtaag tcgtttatta tcagcaatcg tagaacaaca ttcagatgaa
     4081 caaggaatca attggccaaa aggaattgcg ccatttgatc tgcatatcgt ccaaatgaat
     4141 ctaaaagacg aataccaaac aagcctgacg aatgaactag aagaagcgat gacaaaagct
     4201 ggctatgaag tactggttga tgacagaaat gaacgcgcag gagtgaaatt cgcggattct
     4261 gatttgattg gctgtccaat cagaatcact gtcggtaaaa aagcagtgga tggaatcgta
     4321 gaagtgaaaa tcaagaaaac tggcgaaatg gttgaagtac gtaaagaaga attggcgaat
     4381 actttaccaa tcctcttgaa ccaaggcgaa taataagcgt taaaacacag aaaataaaaa
     4441 tagctgaatc agctgttttt gacaatctga tgattcgctt ttaaaattgg aactgcgatt
     4501 tgaatcgcag ttccaatttt ttaatgtaaa actcagaaaa aacgagtata ctaaaaggta
     4561 ctgaaaaaga agaggataag atgcgatcaa ttgcattgtt tctcatcaga aagggcgtat
     4621 atctttgtca gaaaaacgag atttatttga aaaactgttg gaacaaatcc aactagagga
     4681 agcagaaaaa aatcatccac tgcttactgc tggtgaaatg gatagtgtag tggtccatcg
     4741 taaatcgcgt ttatgggaat ttacgctaca tttttccaaa attttgccta ttatgctata
     4801 tcggtcattg actcagcatc tgactatcgc ttttaaagat attgcacaag tcaaactgaa
     4861 tatcttagca gatgaccaga cattcgatga acagctcttg caagattact gggcccaagc
     4921 tttggaaaat cagcaatgcg atacaccact agcacagcaa gtattgaaaa cgcaggtacc
     4981 tgtgataaaa gatcgtaaag tcatcttgcc aatcgacagt acaggagcta tttcttattt
     5041 gaagcaacaa tacttgccat tagtagaaga gttgttcgtc agttacggat tcccaaaatt
     5101 tcgtatcgaa ccagaagtgg atgagcagca agcggaacgt gtattgaaac tttttgaaga
     5161 acgcaaacaa gaacaggcag aagcctttat gaagcaagca gctgaaagtc tagttgtcca
     5221 cgaacaaaag aaaaaggaaa gaaaagagca atcgccagct cttgaaggac ccatccagct
     5281 aggacgaaat attccagccg atgaaccaac gacaccaatg atcagtatcg ttgaagaaga
     5341 acgaagagtc acacttgagg ggtacgtatt cgacaaagaa gtcagagaac tacgttcaaa
     5401 acgcaagatc ttgactttga aaatcacaga ttatacttca tcatttatcg tcaaaaaatt
     5461 ctcgaacggc gaaaaagatg aacaagtatt tgatgcgatc agtgtcggta gctggttaaa
     5521 ggtacgagga agtattcaag aagatacgtt tgtccgtgat ttggtcatga atgcgcaaga
     5581 tatcatagaa gtcaaacata cacctcgcaa agattatgct cctgaagggg aaaaacgagt
     5641 agagcttcat gttcatagca atatgagtac gatggatgca acaaacagca tctctgatct
     5701 tgtggcacaa gcaggaaaat ggggacaccg agctatcgcc attacagacc atggtggtgc
     5761 gcaagccttt ccggaagcac atagtgctgg aaaaaaagca ggtgtgaaaa tcttatacgg
     5821 cgtggaagcg aatgtcgtag atgatggtgt cccaattgct tataatgatg cacatgaagc
     5881 attgagtgaa gccacatacg tcgtatttga cgtggaaacg acaggattgt ctgcagtata
     5941 tgacacgatc atcgagctag ccgcagtcaa aatgtataaa ggaaatgtga tcgaaagctt
     6001 tgatgaattt atcgatccag gacatccatt gtcacgaaca acggtagatc tgactggtat
     6061 cacggatgaa atggttcgag ggtcaaagtc ggaagaagaa gtattgcgga tgttccttga
     6121 attttcaaaa gatacaattt tggtagccca caacgcggct tttgatatgg ggttcttgaa
     6181 tactagctac gcaaggtatg gtattcctga agcggccaat cctgtcattg atacgttgga
     6241 attagctaga tatctgtatc ctcaattcaa acgtttcgga ttaggtgtgt tatctaaaaa
     6301 gtttggtgtt agcttggaac agcatcaccg agcaatctat gatgctgaag caactggtca
     6361 cttagcatgg atctttgtga aagaggcaat ggacaatcat aacatgcttt atcatgatca
     6421 gctaaatgag catatcggag aaggagattc ttacaaaagg gctcgacctt tccatgtaac
     6481 gattttagca aaaaatcagg caggactgaa agatttgttc aaactgattt caatgtcgaa
     6541 tgtcgaatac ttcgaacgcg tcccacgaat accacgctcc caattgaaaa agatgcgtga
     6601 aaatctattg atcggctcag cttgtgataa gggagaaata ttcgaagcca tgatgcaaaa
     6661 aggggtagaa gaggcaagaa accgagcaaa attctatgat tatattgaag ttatgccaaa
     6721 agcggtctat gcgcctttga tcgagcaaga attggtaaaa aatgaacatg atttggaaga
     6781 aattatccaa aatttggtag aaatcggaaa aagtttggat aaaatcgttg ttgcaacagg
     6841 taatgtacat tatctgaacg aagaagatgc catttaccga aaaattttga tcaattctat
     6901 gggcggagcg aatccgttga atcgtcatag tttgccggat gtccatttcc gaacaaccga
     6961 tgagatgcta actgcatttc actttttagg ggaagaaaca gcgaaagaga tcgtagtaga
     7021 aaatacgaat aaaatcgcag atatctgcga agaagtgatt ccagtcaaag atgaacttta
     7081 tacaccgaag attccaggtt cagaagatga aatatcagaa ttgagttaca caaaagcaaa
     7141 acaaatgtac ggagatccgt tacctgagat cattcaaaaa cgattagaaa aagagttgaa
     7201 ttctattaat ggtaatggct tctcggttat ttatctgatc gcccaaaaac tagtgcataa
     7261 aagtaatgaa gatggctatc tagtaggttc acgtggctct gttggctcta gctttgttgc
     7321 gacgatgaca ggaatcactg aagttaatcc attagcccct cattattatt gtccagagtg
     7381 ccaatattcc gagttttttg aagatggtac gtatggttct ggatttgaca tgccagaaaa
     7441 acaatgtcca aaatgtggtg cacgcttaaa caaagacggt catgatattc catttgagac
     7501 atttttagga ttccatggag acaaagtgcc cgatattgat ttgaacttct caggtgatta
     7561 tcaagcagaa gcccataact atacgaaagt attgtttggc gaagactatg tttaccgtgc
     7621 cggaacgatt ggtacagtcg ccgacaaaac agcatatggc tatgtaaaag gatacgaaag
     7681 ggacaataat ctgcagtttc gaagtgcaga agtcgacaga ttagcaaaag gcgctactgg
     7741 agtcaaacgg acaaccggcc agcatccagg ggggatcatc gttattcccg attatatgga
     7801 tgtctatgat tttacgccga tccaatatcc agcagacgat caaaattcag aatggaaaac
     7861 gactcatttt gatttccact cgattcacga taatgtattg aaattagata ttctagggca
     7921 tgatgaccca actgttattc ggatgcttca agatttatca gggatcgacc cacaaacgat
     7981 ccctacggat gacccggaag tgatgcggat atttgccggt cctgaagtac ttggagtaag
     8041 tcaggaacaa atttattcca aaacagggac attaggtatt ccagaattcg gcactcgttt
     8101 tgttcgaggg atgttagaag aaacgcatcc tacaactttt gcagaactac tgcaaatttc
     8161 cggactatct catggtacag acgtatggtt aggaaatgcg gaagaattga ttcgacgagg
     8221 agatgcgaca ctggcggaag taatcggctg tcgtgatgat attatggttt atttgatcca
     8281 tgctggatta gatagcggga tggcatttaa aatcatggaa actgttcgta aaggacaatg
     8341 gaacaagatc cctgatgaat tgcgagaaac ctatctatcg gcgatgaaag aaaataatgt
     8401 gcccgactgg tatatcgatt cttgttcaaa aatcaaatat atgtttccga aagcccatgc
     8461 tgcagcttac gtattgatgg ctcttcgtgt cgcttatttc aaggtttatt tccctatttt
     8521 gtattactgt gcatactttt ctgtacgtgc agacgacttt gatctagtct ccatgtgcaa
     8581 aggaaaagat gctgtgaaac aagcaatgaa agaaatcacg gacaagggat tagatgcttc
     8641 cgtaaaagaa aagaatcaac tgacagtgct ggagttagcc aatgaaatgc tggaacgagg
     8701 attcaaattt ggaatgattg atttgtataa atcggatgcc gttaactttg ttattgaagg
     8761 tgatacgttg atcgcaccat ttagagctgt cccaagtttg ggaaccaacg tggcgaaaca
     8821 aatcgttgaa gcaagaaaag acggtccatt tttatccaaa gaagatcttg ccacccgcgg
     8881 taaagtttca aaaacattga tcgaatatat gaacgacaat ggcgtattga aagatctacc
     8941 agatgaaaat caattatcgt tattcgatat gctataagca tctgttttcc tgtctgcttg
     9001 taattttaag cagagtatgg tatagttctt ataggtaatg atactaactg tgagtgagcg
     9061 gatttttcgc tcactctttt taatggaatt acgcacttaa tttttaagga ggcgaaactt
     9121 ttgagtagcg tcgttgaaac agtcacagaa atggtgacac cgattttaga tgaacaaaat
     9181 tttgaacttg tcgaagtcga atttgtaaaa gaaggaaaaa gctggttctt acgagtattt
     9241 attgacaaag aaggcggtat cgatatcgaa gaatgcgcct ttgtcagtga gaaactgagt
     9301 gaaaaacttg atgcagcaga tccagatccg attcctcaag cttatttcct ggaggtatcc
     9361 tccccgggag cagagcggcc attgaaaaaa gaaagcgact atgaaaaggc acttggtgat
     9421 tatgtccatg tctccttata ccaaccagta gatggcgaaa aacaatatga aggatttctt
     9481 caatcatttg atgctgaaca gcttacattg aaaatacgca taaaaacacg agaaaaagaa
     9541 ttgacttttg accgaaaaaa tattgcaaaa gctcgcttag ctatccaatt ttaatcacgg
     9601 agaggaatca gaaaaaaatg agtaaagaaa tgttaaatgc gttagacgct ttagaagctg
     9661 aaaaagggat ctcaaaagaa atcgtgatcg atgcattaga agcagcattg gtttctgcat
     9721 acaaacgtca ctatggccaa gcacaaaacg tcgaagtgga atttgatcag aaaaaaggaa
     9781 agatccatgt ctatgcggta aaagaagtaa cggaagaagt gatggattca cagttggaag
     9841 tctcattgaa agatgcactt ttaatcaatc cagcatatga gatcggtgat acgatccgtt
     9901 ttgaagtaac accaaaagat tttggacgga tcgctgcgca aacggctaaa caagttattt
     9961 tacagcgtgt acgtgaagca gaaagaacga ttatttataa cgaattcagt gcttatgaaa
    10021 aagacatcat gcaaggaatc gtcgaacgcc aggataaacg ttatatttat gtcaatcttg
    10081 gtaaaattga ggcagttctt tctaaacaag atcaaatgcc aaatgaattt tatcaaccac
    10141 atgatcgaat caaagtttat gtttcccgag tagaaaatac gtctaaaggt ccgcaagtat
    10201 ttgttagccg cagccatcca gacctattgc gtcgcttatt tgaacaagaa gtaccagaag
    10261 tctatgatgg aatcgttgaa attgtcagca tcgcccgtga agctggcgat cgttcaaaag
    10321 tggcagttcg ctcaaatgat ccaaatatcg atgcagtcgg tacatgtgtc gggccaaagg
    10381 gacaacgagt tcaagcaatc gtcaatgaat taaaaggcga aaacatggat atcgtcgaat
    10441 ggaatgaaga tccagctgta ttcatcgcaa atgcattgaa tcctgcacaa gtcatggatg
    10501 tgatcttcga tgagacaaat tcaaaggcct gcacagttgt cgttccagac taccaattat
    10561 cgctagcaat cggtaaacgc ggacaaaatg cacgtttagc tgctaaattg actaaccata
    10621 aaatcgatat caaatcagaa tctgacatga cggaatttta tgaaaatcaa gaacaacaaa
    10681 aagaaacaga agaactgcat gatgaagcaa tcgttcagtc agatttaaca gacgatgaat
    10741 atgaaacaat tgcattcaac aatgaaacgg tcgaagaaaa accagaagct taactttagt
    10801 aaggaggcaa agagatgaaa caaaggaaaa ttcctttacg caaatccgtg gtttcaggtg
    10861 aaatgaaacc taaaaaagag atggtccgaa tcacccgttc aaaagaaggg gaagtatcga
    10921 tcgatccaac tggaaaaatg cctggacgag gagcgtatgt ctcacttgag ccggaagaag
    10981 tacaacaagc ttgggataaa aaaattctcg atcgagtact cgaaacaaca ttaacagatg
    11041 agttttatca agaacttctt gactatgtca ctcaccaaaa agcacgtaaa gagttatttg
    11101 gaaaatgaat acagttaatc ggcaaaaagc catgaatctc atcggtctag cgatgcgcgc
    11161 gggaaaacta atcacgggtg aagaactgac cattgctgaa atccgcaaac aaaaagcaaa
    11221 attggtgttt gtggcaaccg atgcaagtga gaatacaaga aaaaagataa aagataagag
    11281 ttcatattat gaagttcctt gctttgagct attttctgaa gcagaaatca cgcagatgat
    11341 cggaaaacct cgcaaggtat tcggaataat ggatgccgga tttgcgaaga agaccaagga
    11401 gctaattgaa ggttaggaag gtgattgcat gggcaacaaa agaatctatg aattagcaaa
    11461 agaactcaaa aaatctagta aagacgtcgt ggaaaaagca caacaacttg gcatcgatgt
    11521 gaaaaaccat atgggtgcga ttactagcag tgatgagaag aaattgcaac aagcgttcaa
    11581 aaacccaagc acgaaacaac aagctgcaca acaagcaagc agaaaaccag ccaatcaaca
    11641 gccgaacgat caacgtaaaa aagaaacaac gaacaatcaa caaaaaaata aaaataaccg
    11701 cagttatcaa gatcgcggtc aagggagcgg tcaagtgaat caaggaaaaa aaccaacaac
    11761 aaaccaaaat aatacgggaa ataccagcag ccaaaatcgt caaggaacga gccaaggcgg
    11821 caatcaaaac cgtactaaca ataacaacaa accaaacaat aacaatcgcg gcaaatttaa
    11881 taataataac cgtaatcgtt ataataaaaa aggtaaaaaa ggcaaacagc aacaatcaac
    11941 taaaccagct gtaccaccac gcaaattccg tgaattgcca gatgtattag agtatactga
    12001 aggtatgaac gtagcagata ttgcgaaaaa aattcatcgt gagccagcag aaatcattaa
    12061 aaaattattt atgcttggtg tcatggtcaa tcaaaaccaa gcactggata aagatacgat
    12121 tgaactgtta gcgacggact atggcatgga accacaagaa aaagtccaag ttgacattgc
    12181 tgatatcgat aaattcttcg aggcagaaga agtcgatcca gataaattag tctcacgtcc
    12241 gccagtagta acgattatgg gacacgtaga ccacgggaaa acaaccttat tggatacatt
    12301 gcgccattca cgtgtaacaa gcggcgaagc cggcggaatc acacagcata tcggtgccta
    12361 ccagatcgat attgatggta aaccaatcac tttcttggat acaccaggac atgcggcctt
    12421 tacaagtatg cgtgcacgtg gagcaagtat cacagatatc acgatcttag ttgttgctgc
    12481 agatgatggc gtcatgccac aaacag
//
LOCUS       DACEXQ010000062        12342 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.062, whole genome shotgun sequence.
ACCESSION   DACEXQ010000062 DACEXQ010000000
VERSION     DACEXQ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12342
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.062"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(1..246)
                     /locus_tag="IXH04_001237"
     CDS             complement(1..246)
                     /locus_tag="IXH04_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851857.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(361..939)
                     /locus_tag="IXH04_001238"
     CDS             complement(361..939)
                     /locus_tag="IXH04_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ1851858.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(1016..1546)
                     /locus_tag="IXH04_001239"
     CDS             complement(1016..1546)
                     /locus_tag="IXH04_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAQ1851859.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(1550..2410)
                     /gene="rlmB"
                     /locus_tag="IXH04_001240"
     CDS             complement(1550..2410)
                     /gene="rlmB"
                     /locus_tag="IXH04_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAQ1851860.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(2403..2798)
                     /locus_tag="IXH04_001241"
     CDS             complement(2403..2798)
                     /locus_tag="IXH04_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAQ1851861.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(2795..4207)
                     /locus_tag="IXH04_001242"
     CDS             complement(2795..4207)
                     /locus_tag="IXH04_001242"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAQ1851862.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(4204..4740)
                     /gene="cysE"
                     /locus_tag="IXH04_001243"
     CDS             complement(4204..4740)
                     /gene="cysE"
                     /locus_tag="IXH04_001243"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAQ1851863.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(5062..6522)
                     /locus_tag="IXH04_001244"
     CDS             complement(5062..6522)
                     /locus_tag="IXH04_001244"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAQ1851864.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(6692..7849)
                     /locus_tag="IXH04_001245"
     CDS             complement(6692..7849)
                     /locus_tag="IXH04_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAQ1851865.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(7883..9253)
                     /gene="radA"
                     /locus_tag="IXH04_001246"
     CDS             complement(7883..9253)
                     /gene="radA"
                     /locus_tag="IXH04_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAQ1851866.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(9316..9843)
                     /locus_tag="IXH04_001247"
     CDS             complement(9316..9843)
                     /locus_tag="IXH04_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAQ1851867.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            10073..10879
                     /gene="proB"
                     /locus_tag="IXH04_001248"
     CDS             10073..10879
                     /gene="proB"
                     /locus_tag="IXH04_001248"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAQ1851868.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            10876..12123
                     /locus_tag="IXH04_001249"
     CDS             10876..12123
                     /locus_tag="IXH04_001249"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAQ1851869.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
BASE COUNT         3304 a         2683 c         2304 g         4051 t
ORIGIN      
        1 ttaaactgct tcagttaaaa attcgatctc aactgtccga gtaagtacat ctgaatagct
       61 gtaagatact cgttcaaaag aattttcttc aggatccaaa tcaacaacga atactgaagg
      121 ataggtttct gttaaaacgc cgcggcgttc tgtttgacgc tttcttcctg tctgagcaac
      181 caacatgatt gggctgccga tacgaccttc taaatctttc ttgattgaag ctaaagttgt
      241 tggcatacag ttcacctcta ctatcaacta taccacatat ttttaaaatt ttcaagttta
      301 ccataaaaaa aaaagaaatg caataaaaat aaataagaag ctcattaaga tttttctaaa
      361 ttattcctca ttataaaatt tcaagtgttt ttttattttg agttttactc tcccgtaagc
      421 gctcattact tgcttgtgat ccattgctaa acattcagcc atgtctacaa aatcaacgcc
      481 ttgcatataa taatgaaaaa ttttagcttc tatttttgaa aactggattt cgcaattttc
      541 gattgcttct tttaataaaa gatgctgaat cgctaaaggt tcttgcaatg tgatctctgc
      601 taaaaagtct ggtccttctg tctctatttt ttggtcgatt gagacttcat ctttttgatc
      661 acgacgcttc agcgcgttct gcttcctcaa cagagaatag atttgattgc ttataatgct
      721 tttataataa cttccaaagc taacaccttt gcttttttcg taagttctca atgcctgata
      781 taaagcgatc cttgcttctt gttcccaatc ctcagaaaca taatttctta aataataggt
      841 gtttttcaaa cgtagtaaaa gtggaaaata tttttgatac agggcagcaa atgcttcttc
      901 gtcaccatta cgtgcagcgc gcaataactc ctcagacata aaataattcc tccataaatt
      961 cgtttatgga ggaatcttat catcaatgat tcatcgaact gattagaacg atatgttatt
     1021 tattttttct cattaaatcg tccaattttt gggacaattt ttttaactgc tcttcattcc
     1081 atggagaatt acgtcggaaa tcaataaaat gcatatcttt attttcttgg gctattgttt
     1141 tttccgtttt ttttatcgtc ttatataatt cataagcaga agttcgtaat gctccttggg
     1201 aaaagatcat ccactgttct gctaaatcgc ttgtcgccac tgtcacttgt gtcaaacggt
     1261 cattcagctg accagcgatt ctctcgatat aactgtcagc cgtttcgtct tctttcgtaa
     1321 aaacaacatc caactggtat ttcttataat tttgttggat gcccggaact agttgggcat
     1381 cgaagacaac gatgacttcc attccttcat atttcgcata atttgataaa cgatgtaaaa
     1441 gagcttctct tgcatccgcc attttgtcct gctttttaag ctggacaagt tccggccacg
     1501 aaccaatcat gttgtagccg tctacaatca acagctgttt tttcatggtc tactccccct
     1561 gacgttggga aaaagcctga tacataagca aaccagcagc aacactggca tttaagctct
     1621 ggacgtgccc ggtcatcggg atcgtcagca attcatccat ttcttttttc aatcctggac
     1681 tcattccttt gccttcatta ccaatgacta aagcaattga tcctttggca ttccattttt
     1741 ggaagtttgt gccggtcata tctgtgccaa atacccaaaa tcctgtttct ttgagctgtt
     1801 gcacagcttg taccaaattc gtcacgcgtg caacagggac atgttcgact gccccagtgg
     1861 aagtctttgt tacgataggt gtgattccaa ctgctcggtg ttttggtatg atcacaccat
     1921 cgacgccact tgcgtctgct gtccgtaaaa tcgacccaaa attgtgtgga tcttcgatgc
     1981 tatccaaaat caaaaagaag gggtcttctt ttttctcttt tgttcgttca atcagttggt
     2041 caagggataa atattcataa ggtgtaatcg caagaacgac tccttgatgc acgccgtgat
     2101 cgctcaaagt ttctaatttt tgtttaggaa cccatttgac tggcacagct tgttcacgtg
     2161 cgatttcttt cagttcttct actttttctc ctcgtgaatc ttcttgaaga aagagtttat
     2221 tccctcgccc ttgttgtagc gcttctactg cggcatggtg cccaaagacg aaattatctt
     2281 ctaatgcttc ttcattttga tcagaaactg actgatttcg tccagtttgc tttttatttg
     2341 aatatctatt ttgttttttg tcttcaaatc cacgtttcga ttgatgtttg tttgttcttt
     2401 ctttacccat ttttttcacc aactttctga atacaccagc ggatcaattc ttccatccgc
     2461 tcggtttgtt ttgtcaaatg cagatagccc atgagcgctt cgaaccctgt tgcgacccga
     2521 tacgtcgtaa catcagcatt ttttgcgctc gtatggcttt tagcatttct tcctctgcga
     2581 taataaagtt cttcttcttc tgtcaaaaga tgttcttcca gcatttgctg aatcaaaaag
     2641 gcttgtgcat tcgcagaaac ataattcgtt gccgcacgat gcagcttatt gggttttgtt
     2701 tctccttgat caatcaggtg gtcacgaaca tagatttcgt aaatcgcatc tccaacataa
     2761 gccaaggcta aaccgttcaa ttgtgtgtaa tctctcatgc ttctcttctc catcgggttc
     2821 cttgcggtgt atcttctaat aagatccctt tttctttcaa caaatcacga atttcgtcgc
     2881 ttcggacaaa atcacgattg cttcttgctt gatttcgttc ttcgatcaat tgttcaatct
     2941 cttcatctaa taactcttct gctgtaaata aaataccgaa gatcgaaagc cattgcgaga
     3001 ataaagcttc tccttgtgcc aagacagcgg cagaaacttt cggctggtct agataagtgt
     3061 tcagccactt agcaagctca tagaccactg tgatcccatt tgctgcatta aaatcatcgt
     3121 ccatttcttc gatgaatcgc tgttctaaat tttctaacaa agcgatttta tccgcatcat
     3181 ctgccaaatt ctctgaggca gtttcttggc ggaatttcaa attgtcaaac gtattcttca
     3241 gtcgttgtaa attggtggct gcttctttca tcgtcgtttc actgtagcgg atcggacgac
     3301 ggtattgtgt ggtcgccatg aagaaacgca agacttgtgg atcaacgttt ttgatcattt
     3361 catgtacagt gatgaaattc cctagagatt tactcatttt ttcatcgtct tcgccaatcg
     3421 ttacatagcc attgtgcatc cagtaattag cgaatttttt ccctgttttc gcttcacttt
     3481 gtgcgatttc attttcatga tgaggaaatt ctaaatcttg cccgccacca tggatatcga
     3541 tcgtttctcc taaatgtttt gttgccataa cggaacattc aatatgccaa ccgggacgtc
     3601 ctttgcccca tggtgaatcc caagaaatct cgccaggttt tgcttgtttc cataaagcga
     3661 aatctaatgg gtcttctttt tgctgctgtt cttcccctgt acgctgactt gctccgactt
     3721 ccagttcatc gatggattgg tcacttaatt tcccataatt ttcaaattta cgggtacggt
     3781 aatacacatc acctgaagat tcataagcat agcctttttc aaccaacact tgaataaatg
     3841 ctaaaatatc tggcatatga tccatgactc ttggatgcag tgtagcaggc tcgacattca
     3901 acgctttcgt atcttcttca aatgctcgga tgaaacgatc agcaacttct ggtgctgtaa
     3961 tacctaattc gtttgctgta cggatgattt tatcgtctac atccgtgaaa ttcgatacga
     4021 aatcgacttt atagcctcga tattcaaaat agcgtcgaat tgtatcgaaa gcaatggtgc
     4081 tacgagcatt tccgatatgg atataattat acacagtcgg tccgcagaca tacataccga
     4141 catgtccctt ttgtataggg atgaattctt ctttttttct ggtcaatgta ttgtagattt
     4201 ttatcattct gatctcctca ctttttctcc tttgattcga acgacttttg ctggtactcc
     4261 taccgctgta gcgtcgtctg gaacatcttt caacacaacg gcacctgcac cgattttggc
     4321 gttttttccg atcgttaccg gtcctaatat ctgcgcattc gcagagagca tggctccttg
     4381 tttaacagtt gggtgtcttt tacccgtttc ttttcctgtg ccgcctaatg ttacgccatg
     4441 gaataaaatc acatcgtcct cgatttctgc ggtttcgcca atgacgattc ccattccatg
     4501 gtcgatgaac acgccagttc caatcgttgc tcctggatgg atctcaacac cagttaggaa
     4561 acgccagaat tgagcattga tcttcgccaa taaaaagagc cggtgctgat ataaaaaatg
     4621 agagaaacga tgccaaaaca aggcatgaaa accagggtat gtcaaaacga tctcagctgt
     4681 cgaacgtgca gccggatcgt ttctctttgc tgcatcaatc gctcgggtaa accaactcat
     4741 gactttccac tcctttcgta agaattttat gtaggaaatc atcttcttat aaagaaaaga
     4801 agcgcctttt aaataagttt ccttatcaaa agacgctttc cgcgtggttc cacttttctt
     4861 cataatcaat gattatctct gacagctaac gcctgttata cgttcttggc tactttgttt
     4921 cctataaaac agtttttcct gttttctgtg tttcgccaag accactccca gaggcatttc
     4981 ggcaaccatt cattgatacc ttgcaccacc cggtatctct cttgtaatga tcagctgctt
     5041 acttttttct gttcatcgtg tttagttcag tacttgattt aaatgtgcta atgatttttc
     5101 acgaccaagc aattcgatcg tttcaggcaa ttctggacca tgcatttgtc ctgatacagc
     5161 tacacggatt ggcatgaaaa gattttttcc ttttacgcca gtttcttttt gaacagcttt
     5221 gatggcagct ttgacagttg gagcatctac tacttccatt tgttctagct gtgctttgaa
     5281 tgcgtggaga acagtaggaa ctgtttcacc agcaagtact tcttttgctg cttcattcaa
     5341 tacaggatgt tcattgaaaa atagcgtaga cagatcaacg atttctgcag catagctcat
     5401 ttgaggttga tacaggctaa cgatcttctt cacccattcg attgtctttt catctgggtt
     5461 ttctgctaca cgtccatcag cgattagata aggcagacac atgtcagtaa atgcatctag
     5521 atccattgct ttgatgtaat ggttgtttac ccattgcagt tttttcgcat cgaaagctgc
     5581 gggtgattta cttaggcgtt ttggatcgaa cattttgatc aattcttggt gttcgaagat
     5641 ttcgtcttcg cccacaggtg accagcccaa taatgcaata aagttgaaca ttgcatctgg
     5701 tagatagcct aattcgcgat attgttcgat aaattgcaag attgattcgt cacgtttgct
     5761 tagtttttta cctgtttcac tgttgatgat caatgtcata tgtccgaagg ttggtggtgt
     5821 ccagccaaat gcttcataga tcatcagttg ttttggtgta ttggcaatat ggtcatctcc
     5881 acgtaaaacg tgagtgattt tcatcaaatg atcatcgacc gcaacagcaa aattataagt
     5941 tggcatacca tcgcgttttt gtatgacaaa gtcgccaccg atattatcgg attcgaacga
     6001 aatagcgcct ttcaccatat ctgtaaatgc gtattctgta ttgcgaggca cacggaaacg
     6061 tacaacagat tctagacctt gtgcttcttt ttctgcttgt tcttcaggtg tcaagttcgc
     6121 acatgttcct gcgtaatgag gcatttctcc tcttgcgcgt tgtgcttccc gttctgcttc
     6181 taattcttct tctgtgcaat agcatttgta tgctcgattg ctagcaagca gctgatcaat
     6241 cagcggttga tagatttctt tacgttcaga ttgacggtaa gggccgtatt ctccaggatt
     6301 ttcaggagat tcatcccaat tcattcccaa ccaagcaagg ttttccaact ggcttttttc
     6361 gccatcttcg atattacgtt tttggtcagt atcctcaata cgaataataa agtctccatc
     6421 attgtggcgg gcaaacaaat aattgaataa tgcagttctt gcatttccaa tatgcaggtg
     6481 cccagttgga cttggtgcat agcgtacgcg tactttcgtc attttcttcc tcttctttca
     6541 aactagactt tgactaacag caaaaattcc gtcagttacg gcttctcaaa tatcatttat
     6601 ttaaagccac tcagcacaaa ttttaccttt tctaaggtaa atagtaaagc caactggttg
     6661 aatttcctta ccagttggct gaatttcttt tttattttga ttgtttagtc tttttctctg
     6721 tctctttttt attgtcgatt cctcgtcctg catgtgcagg tttagcaaag atcatgcgtc
     6781 cagcagctgt ttgaagggca cttgttacaa cgacttcgat acgttcattc atgtaatgct
     6841 gtccatcttc tacgacaacc atcgtgccgt catcaaggta ggctactcct tgttggcgtt
     6901 ctgtcccggc ttttactact agaacattca tcgtttcacc agggatgacg actggtttta
     6961 cggcatttgc caacgcattg atattaagaa caggtacatt ttggaattca gagactttat
     7021 tcaagttata gtcattggtt acaatgacac catctaaaag cttagccaat ttgatcaatt
     7081 tgctgtctac ttcactgata tcttcaaagt ctccatcgta catttcgaca gatataccct
     7141 cttctttttg caaggcattt aggatatcta atccgcgacg accacgcaca cgtttcaaac
     7201 tatcccctga gtccgcgatg tattgtagtt cgtaaagaac aaaattaggg atcatcaagg
     7261 ttccttctaa gaatccagtc tttgtaatat cataaatccg accgtcaatg atcacgcttg
     7321 tatccaagat tttatattta tggaaatttt ctcctgattt tcgttctaac agttgactct
     7381 caacttgact agctgtttct tgctcgattt ttttgcttcg tgaaccaaaa actttcttcc
     7441 attcttcgat tcgtgtcgtt cccattctca atcccagata gcctaagatg atcatcagta
     7501 aaatcggcaa tacactattg acgaatggaa tttccatatt gtaaagaggg gtcgagatca
     7561 caacacctaa agtcaatccg atgatcgcac cgatcgcacc gaagagaaga taagtcaagc
     7621 tgacctcact tagtttttgt tctacttgtt tgataatagc tgagatatgt ttggccaaca
     7681 gcaaagataa taaaaagaaa ataagtgcgc ctaacaggct gttggtaaat gtattattca
     7741 gccatccgtt tgactgttga ccgattgcct gccaagctga gggtaaaaat gaaataccca
     7801 agcttgcgcc gacaatgatc atcagcaaag taatgacacg tttttgcatc gtttttcctc
     7861 cgtttcgaca atcatttatt agttagcgga acacacgctt caacgtttca gccaaagttg
     7921 ctactccaac tatctcgatt ccttttggcg cttcccagtt acctaagttg tttttaggta
     7981 ggtatacttt tgtgaagccg agtttttgga cttctttgac tcgttgttca atatgactga
     8041 cacgtctgat ttctccagtc agtccaattt caccgatgaa acattccgtt ggctgtgttc
     8101 ctttttcttt atagcttgaa gcaatactga cagcgatagc taaatcaatc gctggttcat
     8161 ttaattttac cccacctgca gcttttaagt aagcatcttg attctgaaga agcaacccag
     8221 ctcttttttc cagcactgcc atgatcagag atacccgatt gaaatcaaga cctgttgttg
     8281 ttcgtttggc atttccgaac atagtaggtg tgactaatgc ttggatctct accaaaattg
     8341 gacgtgtccc ttccattgcc acaacgatag cagagccagt tgcaccgtct aggcgttctt
     8401 ctaagaaaat ctgtgaagga ttagcgactt cttccaatcc atgctcatgc atttcaaaaa
     8461 tcccaatctc attcgttgag ccaaatcggt tttttactgc tcgcaaaatc cgaaaactat
     8521 gatgttgttc accttcaaaa tataacaccg tatccaccat atgttctaac attcttgggc
     8581 cggcgatcga gccttctttg gtaacatgac cgacgataaa aatggctatc ccatttgttt
     8641 tagcgatttt gagtagttct gcagtggttt ctcttacttg gctgacactt cctgccacac
     8701 ttgtgatgtc tggctgcgtc atcgtttgga tggaatcaat gataacataa tcaggggaga
     8761 tatgttcgat ggcacgactg atttcgttca tatctgtttc agcgtataga taaaattctg
     8821 tgttgatact tccgaggcgt tccgcgcgta attttatctg ctctgcgctt tcttcgccgg
     8881 atacatataa gacttttccg cctatggctg ctaattgctg agaaacttgt aacagcaaag
     8941 tggatttccc gatacctgga tcgccaccaa tcaagaccat agaccctggc accacacctc
     9001 cgcctaacac acggttcagt tcttctaatt ttgttttgat ccgaggttct tttttaggga
     9061 cgacgtctgc gatttttgtt ggttttgctt tttctcctgt caaacttgtc cgagttctgc
     9121 ggtccgttgt atcttgttcg atttcttcga ccatcgtatt ccattttccg caatttgggc
     9181 agcgtcccaa gaacttaggc gacacatacc cgcaattttg gcaaacaaat tgtacttttg
     9241 atttttttgc cattgtaccg cctcctttta tcctaatcat gagacattaa atcaagctta
     9301 agtatcttta acgttttatt tactggaaga accaaatccg ccttgacgtt gagcagtcgt
     9361 ttgatcttga tctgctttta aaaacggcaa aaagatccct tggccgatac gctcgccttt
     9421 tttgatcact acatccttga aaccaaagtt ggtgaattgg aacatgatat ggccttcatt
     9481 tgcctcatta ttgtaataat cactgtcaat cacacctact ccgtttgtca acactaaaaa
     9541 ccgcttcaac ggattgcttg aacggttagc aagctgcaaa aattcatctt ctcccatgta
     9601 agctttgatt ccagtaggca cgagggttgg tttcaattgt ttttcatttt ctgatacatc
     9661 ttttttcaat ccatctgcga ctgtttccaa cccctttttc cagcctgctt tccagatact
     9721 tggaatcacg acctcttctg ctgcttcaaa atcatagcca gcagcatgat gtgtagcgcg
     9781 ctgcggtaga ttgatatttt gatctttata tttagaaata acttcaaatc ctctttttct
     9841 caccggtttt cctccctaaa taaactactc ctattctaac atatccgagg ctgtaaaaga
     9901 ctttctttac aaattcttca agaaattctg ctatcttaaa gtaaaccatt aattcgttct
     9961 ttttccaaac gactgcaagt tgtacaaaaa aactattttt tacatgactt tactcgtcaa
    10021 aacaagtaga atagaacgaa attaatgagt ggatatttga ttgaggtgtc acatgcgtaa
    10081 agaaattaaa aatgcaaaac gaattattgt caaagtagga acaagtacat tgatttatcc
    10141 caatggaaat atcaatcttg cggcaatcga tcagttagct tttgtccttt ctgatcttag
    10201 aaatcgggga aaggaagtga tcttagtctc atcaggagct gtaggcgttg gtatgcataa
    10261 attacatttg tcagaacgtc cgactttgat tcctgaacag caagctgcgg cggctgttgg
    10321 tcaagccgaa ctgatgaata tctataatca aagattcagt gtatataatc aacagacggc
    10381 acagatcctc gtgacaagag acgtggtaga ttatcctgaa agtcgtaaaa atgtgaggaa
    10441 tacattggaa cagcttttga ttatggacac gatccctatc gtgaacgaaa acgatactgt
    10501 agcagtggat gaattagatc atctgacaaa atttggcgat aatgatcagc tgtcagccat
    10561 catggcaaaa cttgtcgcgg cagacctttt gatcgtcctt tctgatatcg acgggtttta
    10621 ttcagataat ccgactacaa atcccgaggc tcagatgtat cgctatatcg ataaaatcga
    10681 tgaaaagatt ctggcacaag caggtggtgc tggtagcaca tttggaacag gcggtatgca
    10741 gagtaaatta aaagccgctt ctaggatatt tgaagcaaac agcgcaatga ttttagcaaa
    10801 tggaaaaaat ccggcaatta tttttgatat tcttgcagga aaagaagtag gtactttatt
    10861 taaggaggac ttgaaatgac tgacttgatg caattaggac aacaggcaaa agatagcgcg
    10921 caacaactag cacttatgcc tacaaaccgc aaaaatgagc tacttgttca aatggcacaa
    10981 accatcaaaa agaatcaaca agcgatcatt gaagcaaatc ataaagactt agaaaaagca
    11041 gtcgaaaaca atatcagtga aacgatgcaa gaccgcttgc agctgacacc tgagcgaatc
    11101 aatgccatgg ctacagagat cgaaaaaatt gcttcgttag acgatcctat tggaaaagta
    11161 gatgagatgt ggacgaacac cgacggattg agaatcggca aaaagcgcgt gccactaggc
    11221 gtgaccggca tcatttacga atctcgtcca aatgtcacga cagatgcagc tagtctagca
    11281 ttcaaatcag ggaatgccgt tatccttcgc ggtggcaaag aagcattctt ctcgaatcaa
    11341 ttattagtcc aattattgca gcaagtactg gtcagtgctg gcgaatctcc ttatgccatc
    11401 cagtttgtcg atgatacttc tcatgaaact gcaggacagt tgatgcgatt gacagaatac
    11461 ttagatgtgc tgattcctcg aggcggagca aaattaatcc aacgagtcaa agaacaggca
    11521 acggtaccta tcattgaaac aggtacagga aataatcacg tatatatcga taaagaagca
    11581 cagttatcta tggcagtgaa catcatcgtc aatgcaaaag cttcccgtcc atctgtctgt
    11641 aacgcggcag aaacattgct gatccattct gagatcgcac cttttttctt gccagctatt
    11701 gaaaaagaac tggtagaaca cggggtctct ttacgtgcgg atgctcgtgc gttagaatat
    11761 ctagagacgg ctgctttagc agaagacaca gattgggata ctgaatatct agattatatc
    11821 cttgctgtca aagtggtcga ttctttggat gaagcaattg cacatatcaa tcggcataat
    11881 actaaacatt cagaaacgat cgtgacagat agttatgcgg ctagtcaacg tttcttaaat
    11941 gaagtggacg cggcggctgt ctatgtcaat gcctccactc gttttacaga cggatcggta
    12001 tttggttttg gtgcagagat tggtatctct actcaaaagt tgcatgcccg tggtccaatg
    12061 gggttaaatg aattaacttc cacaaaatat atcatttatg gcgatggaca aatccgcgaa
    12121 taatgtttcg tgtgaaacat tattcccgcc ttttgccttt tttctgtaaa ggttctatcc
    12181 aactagaaag gtgtcaatat gtttgaaatt ttaatgattt tttttattta ccttatttct
    12241 ctgaatatcg ctgcttttct gggagtcggt attttatccc ttttctttca attcaaaaaa
    12301 cgaagcatag ggagtcaacg ggaaaaatgg tcgcagtatt tt
//
LOCUS       DACEXQ010000063        12254 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.063, whole genome shotgun sequence.
ACCESSION   DACEXQ010000063 DACEXQ010000000
VERSION     DACEXQ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.063"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..678
                     /gene="acm"
                     /locus_tag="IXH04_001250"
     CDS             <1..678
                     /gene="acm"
                     /locus_tag="IXH04_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAQ1851870.1"
                     /translation="TFKVQEVDQNGNDYVPSGYQKIENGLVVTNENKEVISVSGQKTW
                     EDNENQDGKRPTTITVNLLADGQPIQHKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTE
                     DNVPEYSTTIDGYNIKNSYTPEKTNISIPGSHITPWKNFPEKTEREKIRNLFSQKLQI
                     FAHTGESSEQRSNYISKRAVPKQITNKSKSILPKTSSKKSSFAVIIGLLIVTICAGIF
                     LQKYKKV"
     gene            complement(950..1435)
                     /locus_tag="IXH04_001251"
     CDS             complement(950..1435)
                     /locus_tag="IXH04_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAQ1851871.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            1915..2556
                     /locus_tag="IXH04_001252"
     CDS             1915..2556
                     /locus_tag="IXH04_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ1851872.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            2575..3210
                     /locus_tag="IXH04_001253"
     CDS             2575..3210
                     /locus_tag="IXH04_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ1851873.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            3228..4043
                     /locus_tag="IXH04_001254"
     CDS             3228..4043
                     /locus_tag="IXH04_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1851874.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            4362..5798
                     /locus_tag="IXH04_001255"
     CDS             4362..5798
                     /locus_tag="IXH04_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1851875.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5987..8434
                     /locus_tag="IXH04_001256"
     CDS             5987..8434
                     /locus_tag="IXH04_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAQ1851876.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            8664..9731
                     /gene="rlmN"
                     /locus_tag="IXH04_001257"
     CDS             8664..9731
                     /gene="rlmN"
                     /locus_tag="IXH04_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAQ1851877.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(9924..11027)
                     /locus_tag="IXH04_001258"
     CDS             complement(9924..11027)
                     /locus_tag="IXH04_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1851878.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            11580..12128
                     /gene="aac(6')"
                     /locus_tag="IXH04_001259"
     CDS             11580..12128
                     /gene="aac(6')"
                     /locus_tag="IXH04_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAQ1851879.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         4136 a         2066 c         2614 g         3438 t
ORIGIN      
        1 acgtttaaag tgcaagaagt agatcaaaac ggaaatgatt atgtcccttc aggttatcaa
       61 aaaattgaaa acgggctagt cgttacaaat gagaataaag aagtcatttc agtctcagga
      121 caaaaaacat gggaagacaa tgagaaccaa gacggcaaac gaccgacgac gatcacagta
      181 aatctattag ccgacggtca accgatccaa cacaaagaag taagtgaaaa agatgattgg
      241 cgctatcgtt tcacaaatct gccaaaatac agagacgggc aagaaatcat ctatactgta
      301 acggaagaca acgtgcccga atacagtacg acgattgacg ggtataacat caaaaacagt
      361 tatacaccgg aaaaaacaaa tatatctata ccaggtagcc atataacacc gtggaaaaat
      421 tttccagaga agacagagag agagaaaatt agaaatctat tctctcaaaa attgcaaata
      481 tttgctcata caggagaatc cagcgaacaa agaagcaatt atattagtaa gagagctgta
      541 cctaaacaga taacaaataa atcaaaaagc attcttccta aaacaagtag taaaaaatct
      601 agttttgctg taatcattgg tttattgata gtaactatct gtgctgggat attcttacaa
      661 aaatataaaa aagtttagtt agaatataat attttttgtt aaatatgact gaaatcaaaa
      721 gaataaaatt ccgatttata atcgaaaaaa gaagcatttt tatatatgct tcttttttcg
      781 tagaatcaat aaatatggat ttggctaact atggaagaat actcgtatta gttttcagcg
      841 ttcatactag ttgttacatt gtttgattgg ccaaaagaat ttctgatata ttttgtgcta
      901 aataaagaaa aagacgcttg aattgggaaa taagaaaact actctatcat tacaaatttt
      961 cataaactgg cagatgttct aagtaaacaa gtatcttttc aaagtcggaa aaatccataa
     1021 tcaacgtagt ggtattgata tttggatgaa agcctacggt atcttgacga tcaatagcag
     1081 catcgatcac gacttggatt tttttctctt tgtcgtgcat caatgctaat ggagtcactg
     1141 tacctgctgg aacgcctagg aggtcaggaa gctgttcttc agaaacaaaa gataagcgct
     1201 tttcttttaa ttgttcagct agttgtttta aatcggcgtg cttttcatca ggcaagatga
     1261 caagatagat ttgttttcct tttcttgatt tcaataatag attttttact tgcggaccag
     1321 gtagggaaaa agggacattt ttgactgacg taatcgcagg atgatcgact ttttgaaaag
     1381 agatacccag tttttttagt agttcataag cttcttgttc ggttgcacga ctcatcgcgc
     1441 atcaccctcc tttttttatt agtgtatcat aaaaaagagt tgacaaatga aaaataatca
     1501 tgtaaattac acgtaaagtt aaaacaaatg acatcgaaaa gaagagtagc cgttaggtgt
     1561 ttttagagag tctctggttg gtggaaagag gcagcagtta accgtgaaac acatcttgga
     1621 gtcgattttc tgaagcgtat agtaggaaaa tccggggagc ccgttacagc tgcggtcgtt
     1681 gctagatgac cagataaggc tggcttttcc gtgaggagtc ggtaaaaaaa ggtggaacca
     1741 cgtgattaac gtcctttgat cttcggatca aaggactttt tgttttcatc tgaacggcaa
     1801 cgactgtgtg aggttttttc tgcaaggaaa aaataaacag aggtggaacc acgtataata
     1861 cgtcctcttg gcatttgcca agaggatttt tttattttaa aggaggaaaa gaagatggag
     1921 tatttgattg aagttatgcc gcaattatta gtaggagcac tcacaacatt gaaattattt
     1981 ttatttacct tgattggctc attaccatta gggattgttt tagcattagg attgtctttg
     2041 cattttaagc cgctacgcta cttgcttcaa acatatgtat gggttatgcg gggaacaccg
     2101 ctgttgttgc aactgatttt tattttttat gggttgccga cgattgggat cgtttttgat
     2161 cgtttcgaag cagcactcat tgcctttatc ttaaactatg cagcttattt tgcagagatc
     2221 tttcgtggag ggatacaatc gattcctcaa ggtcaatatg aagcagcgca agttttgcaa
     2281 ttaagtccct ttcaaacggt gacgaaaatc gtcatcccac aagtgacaaa gattgtttta
     2341 ccttctgtag ggaatgaagt tatcaacttg gtcaaagatt cttccttgat ctatatatta
     2401 ggtttaggtg acgtaatgcg tgctgggaaa atcgcgatgg aacgcgacgc gactttactg
     2461 cctttgctag gcgtggcaat catctatctt acattgactg gtattctaac aatcataatg
     2521 aaacaagttg aaggaaaatt aaattattac cgctaactga taggaggaag aaatatgatt
     2581 gaattgaaga aagtgaacaa agcatttggt gaaaaaaaaa taatcaaaga tttgaatcta
     2641 atcattccag atgggaaaac attagctatc gtaggtcctt ccggtggcgg taaaaccacg
     2701 cttttacgta tcttggctgg cttagaaagt tctgacagcg gagaatttta tttagacggt
     2761 gtaccgttca acccattcga gcttaaaatg aaagaacaag ttgtaggagt cgtattccaa
     2821 gacttccagc tatttccaca tctgaatatt tttgaaaaca ttaccttagc accaaaaatg
     2881 gtattgaaac agacaaaaga agaatacacc aaaaaagcag aagaattagc ggcttcatta
     2941 gggattagtg atttattgaa tatctatcct tatcagcttt ctggcggaca aaaacagcgg
     3001 gtagcgattg ccagagcatt ggctatgaat ccacgggtat tggcttatga tgaaccaaca
     3061 agtgctttag atcctgaatt acgccagcaa gtcgagcgtt tgatcctatc attgaaagaa
     3121 gatggggtaa cccaaattat tgtcacccat gatttagtat ttgcagaaaa tgtcggtgat
     3181 caattactaa aagtttcccc aattcattaa gaaaggagaa gatagagatg aaaaaaacca
     3241 gttttctatt cgctttgatc gcaggtcttc ttctttttgc aggctgttcg aacaaaaaaa
     3301 cttcagcaga taattgggcg actatcaacg aagagaaaaa aatcgttata ggtttagacg
     3361 atacatttgt tcctatggga tttagagata aagacgggaa gctcacagga tttgatattg
     3421 atttagccaa agcagtcttt gaacaatatg ggattacggt agactttcag cccattgact
     3481 ggtcaatgaa ggaatttgaa ctgaacaatg gaacgatcga tttgatatgg aacggctatt
     3541 ccaaaacgcc ggcaagagaa aagaaagtgc aatttacaaa accttatatg gaaaacgatc
     3601 aagtgctgat cacacctaaa gaatcaggaa tcacaaactt tggacaaatg aataataaga
     3661 gattaggtgc gcaaaatggg tcatctggtt atgatgtatt tacgaaacaa ccagaagtat
     3721 taaaagagat agtcaaaaac caagatgctg ttttgtatga tagttttaat gaagcattga
     3781 tcgatctgaa aagcagacgg gtcgatggtc tgctgatgga taaagtatac gcagactatt
     3841 atttgaaaca aagaaacgaa ctagaagact tcaacataac aaaaggttct tatgagagtg
     3901 aggattttgc agttggtgca cgcaagtcag atagtacatt agtgaaaaaa atcaatgaag
     3961 ccctagatca actagagaaa acaggtgaat tccaaaaaat atccgataaa tggtttggcg
     4021 aagatgttac gccgagaaat taacaaatag gggactgtga caaaagacgt gtcacagtcc
     4081 ctttttccga ataaacagtg ttctcctgcc aactccttgt gactcggagc tgaatggcag
     4141 gctcacaacc tcttggtaaa cggtgttcaa aagccagttt ttcggtatta agccaaattt
     4201 atgcaaaaat gtagaaagcc attttcacaa aatttttctt actactcgaa actaagcgac
     4261 tttttcacaa cctcctttac ataattttat tttgttgtgg aaggggtttc gtgttattct
     4321 aaaagaggaa cgattgaagg ttataggaaa ggaagagatg aatggaacac aaagaactta
     4381 cctcatttcc aaaagatttt ctgtggggct ctgcttctgc agcgtatcaa gtagaaggag
     4441 cttggcaaga agatgggaaa ggagaatcag tctgggatcg atttgtacgg atacctggaa
     4501 agacatttaa aggaacgaac ggtgatttgg cagtagacca ctatcatcgt tataaagaag
     4561 atattgcttt aatgaaagaa caaggtctga aagcttaccg cttctctgta gcttggacac
     4621 ggatttttcc taatggacga ggagaagtca atcaagctgg gctcgctttt tatgagagat
     4681 tgatcgacga attgattgaa aacgatattg aacctatgtt gactttatat cattgggatc
     4741 ttccacaagc attgcaagat gaatataatg gttgggaatc acgtcagatc attgaagatt
     4801 tcactcatta tgcagaaaca ttatttgagg cattccgcgg gaaagtccat tactggatta
     4861 gtttgaatga acaaaatatc ttcacttcat taggttattt gttagcagca catccgccag
     4921 gtgtgacaga cccgaaacga atgtatgaag ttaaccatat tgctaattta gcaaatgcat
     4981 ctgtcatcaa taaatttcac gagttagaaa tgcctggaaa aatcggacca agctttgcgt
     5041 atacgccaaa ttatccaatt gacagtaatc ctgaaaatgt tctggctgct gaaaatgcag
     5101 aagacctgat ggccaattat tggatggatg tctatatgtg gggaaaatat ccgattgctg
     5161 ccatgaggtt cttagaagaa aaaggatggg caccaacgat tgaagctggc gatgcagaat
     5221 tgctagaatc tgctaaacca gattttcttg ggatcaatta ctatcaaacg gcgacgaatg
     5281 cattcaatcc attaaacgga gttggtgctg gaaaaatgaa tacgacagga aaaaaaggtt
     5341 catcagaaga aactggagtt cctggaatgt ataaaaaagt tgagaatcca tttgtcgaac
     5401 gaacaaattg ggactgggag attgacccag aaggattaag gatcggactg cgtcgtatca
     5461 caagccgtta ccgcatgccg gtactgatta cggaaaatgg cttaggagag tatgacaagt
     5521 tgacagaaga taaacaaatc catgacgact atcgaatcaa ttacttacaa agccacgtca
     5581 aagccatcaa agaagcaatt tcggatggag cagaagtcct aggctattgc acctggtctt
     5641 atacagattt gcttagctgg ctgaacggtt atcaaaagcg atatggattt gtatatgttg
     5701 atcaagacga aacacaagaa ggatctttgg aacgatataa gaaagacagt tattactggt
     5761 accaaaaact gattgaagaa aatggagaga acctttagtt cacatactta aaacaagaag
     5821 gtacggctgg atatggccgt gccttttctt gataaaatca tttagaaatt aagaaaaact
     5881 gtaaaataag ctgaaaaatg aaagaaagcg tcagttcttt caaacaaaac tttagttccc
     5941 ttactttttt ttgtataatg aaacttgatg aaatgaggga taataaatgg cacaaaagaa
     6001 acggcctact aaaaaaagga aaaagacgaa aaaaacgaaa aagcaaaaac agcagcagga
     6061 acaaatgctg acgattggtg ttgggtttgc cttgatttta tttgctgtct tcggcttttt
     6121 aaaattaggt tttttaggaa tattgattgc aaacggcttt cggatcattg caggaaatac
     6181 ttaccaaatc ctctgtcttt tattagccgt attaggattt tggattgtca ttaagaatac
     6241 agagttttca attggtaaaa accgacgctg gtttggcggc atactctttt atttcggtat
     6301 cttattattg ttacatgccc atttatttgg caaactccat actggggaac caaatatcat
     6361 ggggacgaca tgggatttgt tagccagtga tataaaacaa agtcaagtag acaataacgt
     6421 tggtggtggg atgattggag caatccttta tcattttact tacttcttga ttgcacagcc
     6481 tggaagttat ttagcagcag tgctattact ggcaggagga gcattcctgt tcagtaatct
     6541 ggaaggctat cagctgttaa atggtatcca atcaattgga gaacgagtac aggaattatt
     6601 ggaaggtgat cctgccaagc aagctcgaaa gcaagcggca aaggaagaga gaatgaaaca
     6661 gcgcgcagaa gcgaaagaag caaggcgctt agctgcaaaa gaagctgcag aaaaagaagc
     6721 tgcagaatat gagaagaaaa aagcggtgag ccagcaaagg gaaaatactg ctgatgaatg
     6781 gcaggaacca gaaaatacag agccggagca gctaagtttt gttccaatcg acagttttca
     6841 agaaaatatc catcctgcga atctagagaa accagtacca gatacaccaa aacagaccaa
     6901 tacagcagaa ggtttttcag atgagttgcc agaggatgac ggtacctcat tagaatttga
     6961 gattgaagca gaacaggaaa atcaagacta tgaattaccg acagttgatt tgcttgattc
     7021 tataccaact gtggatcaaa gtgatgagta taaaaaaatt gaaaaaaata tcggcgtatt
     7081 agaacaaact ttccaaagtt tcggtgtcga tgccaaggtc gtcaaagcca gcttaggacc
     7141 atccgtcaca aaattcgaag tccagccggc tgtaggggta aaagtaagta aaattgtgaa
     7201 cttaacagat gatatcgcgc ttgctttagc ggcgaaagat gtccggatgg aagcaccgat
     7261 tccaggtaaa tcactgattg gtattgaagt accaaatgga aagatcagta tggtttcctt
     7321 tagagaaatc atcgaagcac aaccaaacca cccagataaa ttactagaag tacctttagg
     7381 acgtgatgtt tctggacggg tacaaacggc tgatctttcg aagatgcctc acttactcgt
     7441 ggctggttcg actggtagtg ggaagtcagt agcaattaat gggattatca caagtatatt
     7501 aatgcgtgct aagccccatg aagtaaaact gatgatgatc gatccgaaaa tggttgaatt
     7561 gaatatgtac aacggcatac ctcatctgtt gacgccggtc gtgacgaacc ctagaaaagc
     7621 agcgcaagcc ttacaaaaag tagtacaaga aatggaagaa cgatatgaaa aattcgcggc
     7681 aaccggagta agaaatattt ctggttacaa tgaatttgtt caacagaaaa acttggaaaa
     7741 cggaacgaaa catccaacct taccattcat cgtagtgatc gtagatgaat tagctgattt
     7801 gatgatggta gccagcaatg aagttgaaga tgcgattatt cgtttagcgc aaatggctcg
     7861 ggcagcgggg attcatatga tcctagctac tcaacgtcca agtgtggatg ttatcacagg
     7921 aatcatcaaa gcaaatgtac cttccagaat ggcatttgcg gtatcaagcg gtacagattc
     7981 tcggacgatc attgattcta atggggcaga aaagctctta gggcgaggag atatgctatt
     8041 cttgccaatg ggtgagaata aaccgatccg tgtgcaagga gcattcattt ctgaccacga
     8101 agtagaacgg gtcgttcaat ttgttacaga tcagcaagaa gctcattatg aagagaaaat
     8161 gatgccgaca gatgaagttg aaacgacagg tgctccagaa cagccacaag atgagctgtt
     8221 tgaagaagca aaagcacttg tcgttgaaat gcaaacggca agtatttccc ttctccaaag
     8281 aagatttcgg atcggttaca accgagcagc acgtttagta gatgaactgg aagctcatgg
     8341 agttgttggc ccgtcagaag gaagcaagcc tcggaaagta ttcattgagc aggaagaaac
     8401 atcagaaacg ttgccggacg ataatgacat gtaaaacttg tgtgatgaag ggttgaaaaa
     8461 cgaataacca ataaaaagac aaggcatgat gataccaaaa gaaaaaatgg tttttttcca
     8521 tcttttctaa tacggtatga tgtcttgtct ttttagtttt tgtgagatta aagttctatt
     8581 cgatttattt gtgaagtatt gcattaagga aatgaatcat gtatagtatg tagcgaaact
     8641 ttgagaaaaa gagggaaaat actttggaaa aacagtccat ttatggcttg acgaatgaag
     8701 aattaatcaa ttggtttata gaaaacggcg agaaaaaatt ccgagctgcc caagtttggg
     8761 aatggttgta tcaaaaacgt gtaagtaatt tcacagaaat gacgaatcta tcgaaacaat
     8821 tgatcgaaaa attatcggag cattttatca tcaatcctct aaaacagatg gtcgtccaag
     8881 aagcttcgga tggtacagta aaatatttgt ttgaactccc tgataaaaac atgattgaga
     8941 ctgtactgat gagacaagaa tatggcttgt cagtttgtgt gacgacacaa gtaggatgta
     9001 atatcggctg tacgttttgt gcaagcgggc tgctcaaaaa gaaccgtgat ctgactgctg
     9061 gagaaattgt tgcacagatc atgatggtcc aacattattt tgatgagcgc aatcttggcg
     9121 aacgcgtttc acatgtcgtt gtaatgggga tcggtgagcc atttgataat tacgacaatg
     9181 taatggactt tttgcatatc atcaatgatg ccaaaggatt ggcaattggt gctcggcaca
     9241 ttacagtttc cactagtgga ttagcacaca aaatcaagga attcgctaat aacggcttgc
     9301 aagtcaatct ggctatttcc ttgcacgcgc cgaataatga ggtgcgaaca tcgatcatgc
     9361 ggatcaaccg aagtttccca attgagaaat tgatggaagc tgtggatgag tatttagaga
     9421 aaacaaaccg tcgaatcacg tttgaatata tcatgttgaa tcaagtaaat gatagaccag
     9481 agcatgcgca acaattagca gatctattaa aagataagaa aaaattagca tatgtcaact
     9541 tgattccata taatccagtc agtgaacatg atcaatattc tcgcagtcca aaagctgatg
     9601 tattgagatt ttatgatgta ttgaagaaaa acggcgtaaa ctgtgtgata cgtaaagaac
     9661 atggtacaga tattgatgca gcttgcggac aattgcgaag caagcaaatg aaaaaaacag
     9721 ttgccaaata atcgtatcct actcgttttt tcaattacca taaaaggagc agttttctta
     9781 attaaggaac tgttcaactt aaataagaga aaagattcta tagcgaaagc tataaagctt
     9841 tttttgacaa gaaaaaccct catcaagcaa agaaatgtcc tcaatttgat gacagcctgc
     9901 ttgatggggg ttttagcaat tatttaactg attttcttat atccttgctg gctttcttgt
     9961 gtagcatgat aagtttctac gtcaagaatg gcctgtttaa atttttctcg ggtgatttca
    10021 taaggcacat gtttaatatc atttgttgcc atggctttat ctaagattgc ttctaaatcg
    10081 gtcgtgacat tgagattcat gtcggttaac gatactggaa gttggttctt tttcataaac
    10141 acatagattt tatcaaacaa atcgaactgc tgatccatca tcaataaggt cagtacacca
    10201 taactgacag atgctccgtg aaggtatccc tccatttttt ctagaacagt cgtaccgtaa
    10261 tagaatgagt gtgccatagc agaattatag ttattgttaa ctaaaacaga aaccaatccg
    10321 gtagtaacaa tgatatccga aacgacttgt tcaattgcat atgagggacg atcgtcggca
    10381 ctatctttca atgcttgcgt accataagtc aagagacgat caatgcacat ttttccgatt
    10441 tggattccta atctatccgt gtagcctaga tcatcgccac gagcagaaaa agtagattcg
    10501 acttctttgg ataaagcatc gccaatacct gcccataagt atttatcagg tgcttcggca
    10561 ataattgctg tatcaatgaa ggtgtgtaca ggtggttttg aaggaaagaa gagtccgtcc
    10621 attacgcctt cttcgttata gatgacacat accgcagtga cgggcgaaca gttcgaacca
    10681 atagtgggaa aggtgaagac tggtttagct agtttttcac caatgacttt tacagtatcg
    10741 caagcacggc caccaccgac tgcaaacagg acgtctgctt tttggacact ttcatgctgg
    10801 atcaactgtt caatatttcc ataagttgaa ttacccccgt accagagact ggcgacaacc
    10861 tctatttcct gtgtcaaagc tacttttatt ttgttttctg cctttgaaag tgcagtcttc
    10921 cctccaatca gcactacttt tgtcccatat tctttgacga ttccatttgc tttttcatac
    10981 gcatctgttc caatggtata atttggtaag aaaacacttt gactcatatt catttccttc
    11041 tttcttcgtt gaaacgggtt aaaattttaa agagtgatcg atctgactgc ggctgtagat
    11101 ggagtagagg tgaacctaag acaggataag tgatgaataa ggtggcatga acaccttgaa
    11161 atcatcaata agtatcctaa aaacgaacaa gtacaaaaac attgaaaacg acaataatga
    11221 atttttgttc ctcttagtat ttctaccgat tccctttgct tttttgtaat tatctaaaaa
    11281 ttctaaaaat taagattatt atatgaaaac aggttttatt tgtcaattca taattggttt
    11341 tttttaaagc aaaagataat ctatatcatg atgaacaaac agagtttttt cctccaattg
    11401 atggctttcc tgatctttcc tattttgaat aaagtgtttc tgtataattt ttttatttta
    11461 taagtaagat aaataatgaa agaatagaga tactgattgg tagctaaaga tttttgagcg
    11521 agaacatttc gttttttcaa aaggactgtt agaataagca agaacaaggg ggaagacgta
    11581 tgataatcag tgaatttgac cgtaataatc cagtattgaa agatcagctt tctgatttac
    11641 tgagactgac ttggccggaa gaatatggag acagctcggc agaagaagta gaagaaatga
    11701 tgaatccaga acgaatcgcg gtagcagcgg tagaccaaga tgagttagta ggatttattg
    11761 gtgcaatccc tcaatacggt atcacaggtt gggaattgca tccattagtt gtagaaagct
    11821 cccgacgaaa gaaccaaata ggtactcgat tagtcaatta cttagaaaaa gaagtagctt
    11881 ccagaggagg aatcacgatt tatttaggta cggatgattt agaccatgga acaacgttaa
    11941 gtcaaacgga cctgtatgag catacatttg ataaagtggc ttccatccag aaccttcgtg
    12001 aacatccgta tgaattctat gaaaaattag gttataaaat cgtaggtgtc ttaccaaatg
    12061 caaatggctg ggacaaaccg gatatttgga tggcaaaaac gattattcct cgaccaaatt
    12121 ctcaataata aaaagaggtt gtgacagaag taatcagctt caagaaataa gaaggaattt
    12181 acgaaaatta cttcttgaat ttttgtgaaa ttccaactta tttccgaaga agctactttt
    12241 ggaacaccgt gtac
//
LOCUS       DACEXQ010000064        12181 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.064, whole genome shotgun sequence.
ACCESSION   DACEXQ010000064 DACEXQ010000000
VERSION     DACEXQ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12181
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.064"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..645)
                     /locus_tag="IXH04_001260"
     CDS             complement(<1..645)
                     /locus_tag="IXH04_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid utilization regulator"
                     /protein_id="HAQ1851880.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLA"
     gene            621..794
                     /locus_tag="IXH04_001261"
     CDS             621..794
                     /locus_tag="IXH04_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ1851881.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(839..1576)
                     /locus_tag="IXH04_001262"
     CDS             complement(839..1576)
                     /locus_tag="IXH04_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ1851882.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(1578..3080)
                     /locus_tag="IXH04_001263"
     CDS             complement(1578..3080)
                     /locus_tag="IXH04_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1851883.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            3339..5333
                     /gene="uvrB"
                     /locus_tag="IXH04_001264"
     CDS             3339..5333
                     /gene="uvrB"
                     /locus_tag="IXH04_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAQ1851884.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            5343..8159
                     /gene="uvrA"
                     /locus_tag="IXH04_001265"
     CDS             5343..8159
                     /gene="uvrA"
                     /locus_tag="IXH04_001265"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAQ1851885.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            8422..9687
                     /locus_tag="IXH04_001266"
     CDS             8422..9687
                     /locus_tag="IXH04_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAQ1851886.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            9691..10422
                     /locus_tag="IXH04_001267"
     CDS             9691..10422
                     /locus_tag="IXH04_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAQ1851887.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            10557..11441
                     /gene="rapZ"
                     /locus_tag="IXH04_001268"
     CDS             10557..11441
                     /gene="rapZ"
                     /locus_tag="IXH04_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAQ1851888.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            11438..>12181
                     /locus_tag="IXH04_001269"
     CDS             11438..>12181
                     /locus_tag="IXH04_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAQ1851889.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRV"
BASE COUNT         3910 a         2154 c         2628 g         3489 t
ORIGIN      
        1 agccagcttt agaatatgat aatcgtcata gtttctaggg gtataaccag tgagagaatg
       61 gaacttgttc aatcgatatt gcaaggtgtt tttatgaata aataattctt ctgagcaatt
      121 tttgatgctt ccgttatttt tactgaagca tgccaaagtc ttcttgaaca aatgtacttc
      181 ttcttctgtc aggcttttca aaatacgttt tacatataat gttcgttgat ctgacgggat
      241 tgctggtaaa acgatcccga ggtctaaatc ttcaaaaaag cggatctcgt tcacagattg
      301 atggaaagct atccattcgc aaacttgttt ggcatgaagg aaagattgcg gtgtctcttg
      361 ctctgtataa caaagcgacc caattccaaa gtttagctgt aactgataca ttttattaaa
      421 ctttgtctgg attttatcaa gcaatgatag caatgaggct ctggaaatcc gattgactag
      481 gataatgatt tgttgtctat gtacagtgag caaagcctgt ccgtaataag agaagaccct
      541 ttcgagcaac tccaataaag gattgatccg ttcttgacac aattgctgat tgtcagaaag
      601 ttgtcccaca actacataat atggagaaga aaaatcaagc tcaattaagt gagggtggta
      661 tcgactatgt acttgatatc atcaatgaac cgatggtact ttcttatgcg atggaacagg
      721 tagagcgatt gcttgtttgt gcaggaaaac aagcgatgcg gctgatacga ttaggtgaga
      781 aaataagaag ataaaaatac acggtccctt catggcgacc gtgcattttt ggtttttatt
      841 aaatatttaa tactttattc aagaaatctt ttgtccgttc attttgcgga ttttcaaaga
      901 tttgttgagg cgttccatct tctaaaaagt ttccgtcgtc aatgaacatg acgcgattgg
      961 ccacttcttt agcgaacccc atttcgtgcg taacgataac catcgtcata ccttgttctg
     1021 ctaacttttt catgacgttc aagacatcac cgaccatttc aggatcgagt gcagaagtag
     1081 gctcatcaaa aagcatgatg tcagggttca tcgctagagc acgagcaata gccacacgct
     1141 gtttttgtcc accagaaagc atttcaggat acatatcttt tttatcttct agaccaactg
     1201 tctgcagtaa tttgattgct tttttctctg cttcttctct agattctttc ttcaggtcca
     1261 tcggtgctaa aatgatgttt tccaaaatag aaagatgagg gaataggtta aagtgttgga
     1321 ataccattcc gatatgttgt ctcaccaagt tgatatctgt gcttttatcc atcaagtggg
     1381 cgccgtcaat aataatttca ccgtcagaag gttcttctaa acgattcaag caacgcaaga
     1441 atgtactttt tcctgaacct gaagggccaa tgatacagac gacatctcct tcgttgatgg
     1501 atacattgat gtcgtttaat actgtgttat cgccgtattt tttgactaaa tgctcaacta
     1561 agattttttc tgccatctta cttcaccttc ttttctaaaa cttttgctaa ttttgtcaat
     1621 gctgtgatca aaatcaagta catgatcgca atgatcaagt aaacatacgt actttgtgtt
     1681 gtacgtgcaa caatgatctt accagtttgc agcaattcga ctacaccgat tgcggaaatg
     1741 atcgttgtgt cttttaatga gataacgaac tgattgacga atgatgggat catgatgcgt
     1801 atcgcttgcg gcaaaatgat tttttgcatt gtccgattat aagaaagacc taaacttctg
     1861 gaagcttcca tttgtccgac aggaacggcg ttgatcccgc ctcgaacaat ctctgcgata
     1921 taagcacttg cgttaagagt caaggtgata acaccagcaa ggaagtcagg aatcgtaaat
     1981 ccaacgatac caggcaaccc aaagaaaata aagaacgcaa gaaccatcat tggaattcct
     2041 cggatgatat cgacataaat actagcaatt gtgcgaagag cgcgtattgg tgctacactg
     2101 aataatccaa aaatcacacc gattactata gccaaagcaa aggagatcaa agttaggaga
     2161 attgttctcc aaagtccttt caacaatgct tgatagttgt tttggataag tccaagaaat
     2221 gttgattcat cagcagtgga agactcactg gaagtttctg aaccatccgc tacatattct
     2281 ttaataatct tatcgtattc gcctgttctt tttaattctt tcaatccttc attgaacata
     2341 ttaagaagct caggattttg tcctttttta acggcaaatg catattttcc gccagattct
     2401 tttttgatcg gtgtttccaa ttcttgtcct tgtttgatcg cataaccgat taccggataa
     2461 tcatccatca ttgcatcgat tgcgccaact tttaacccgt cgtacaattg atcagcagca
     2521 tcaaaatatt tgatagaata gccatattca gattcatggt cgcttaaaaa ttcagcactt
     2581 tcggtaccaa cttttgctcc gactgttttt cctttcaaat cttcataaga cttgatcgaa
     2641 gtatctcctt ttttgacagc taattggatc ccgctatcaa agtaagaatc agaaaaatca
     2701 tagcttttct ttcgttcatc tgttacagtc attccggcaa tcattccgtc tgcttgtcct
     2761 gattccaaag cttgcaaagc agcagagaaa ccacggtagt ccattcggat gttaaatcct
     2821 tgcagtttgg cgatgttttc catcaatttt acatcgatgc cttcgtactc gccctttgcg
     2881 ttttgaaatt caaatggagc aaaagcagag tcactggcaa ttacgtatac gtcttttttg
     2941 ggctctattt tttgcattgt ctcgcttgtg cttgactctg ccgttgtagt gttgtcagct
     3001 tgtgcaacat gaccaaacgc aagtgtcaca agtcctgtca ttaagaaaac aaatgagtga
     3061 aataaatgct ttcttttcat aatcaacctc ctaaatttcc aatattttat tattataggc
     3121 gaaaagtgct gaatcagaca agactgtaag ctcgaaaaaa tgaaaaaaat gcgagtttct
     3181 aacattttat gttataaaac ataacatgta taataagata aaaaagtggc gaaagtatgt
     3241 tcggtgtggt ataatttttt agtaaggaat gcctttgctt tccttttctt tttattggtc
     3301 gtaaaatgaa aaggaatgaa tgaaaaggag gacgaagaat gattgagaga gatacctccc
     3361 gacactttga gcttgtctcg aaataccaac cggctggtga tcagcctgaa gcgattaacc
     3421 aattagtgga tggcgtagtc ggcgggaaaa aagcacaaat cttattaggt gcgactggaa
     3481 cagggaaaac atatacgatt tccaatctta ttgagaaagt gaataaacca acactgatta
     3541 ttgcccataa caaaacattg gctggtcagc tgtacgggga attcaaggaa tttttcccta
     3601 acaatgctgt tgaatatttt gtttcttatt atgattatta tcagcctgaa gcttatgtgc
     3661 cttcaagcga tacgtatatc gaaaaagatt caagtgtaaa tgatgagatc gacaaattac
     3721 gacattcggc tactagttca ttattagaaa gaaatgatgt gatcgtcatt gcatccgttt
     3781 catgtatttt tggattaggc tcgccttttg agtaccaaaa acaagtagtt tcgattcgac
     3841 agggagcaga actagatcgc aatcagttga ttcgtgactt agtcagtatc caattcgaaa
     3901 gaaatgatat agattttcag cgcggtcgtt ttcgagtgcg cggtgatgtc gtggagattt
     3961 ttccggcttc aagagatgaa cgtgccttac gtgtggagtt ttttggagat gaggtcgaac
     4021 gtatccgaga agtcaatgca ctgacaggtg aagttttagg agaaacggaa cacgtagcaa
     4081 tcttcccggc gactcacttt gtgacgaatg atgaacatat ggagcatgcc gtagcgaata
     4141 tcaaagcgga attagaacag cgtttaactg tcttgcgaaa tgaaaacaaa cttctggaag
     4201 ctcaacgttt agaacagcga accaattacg atattgaaat gatgttagaa atgggctaca
     4261 cgtcgggaat tgaaaattat tctcggcata tggacggacg taaagaaggt gaaccgcctt
     4321 atacgctatt ggatttcttt cctgaagatt ttttgatcgt ggcggacgaa tcgcacgtaa
     4381 ccatgcctca aatcagagga atgtataatg gtgaccgagc gagaaaacag atgctagtag
     4441 attacggttt ccgtttgcca tctgctttag acaatcggcc acttcggtta gaagagtttg
     4501 aaaaacatgt caatcagatc atttatgtct ctgcgacgcc aggaccatat gaacatgaac
     4561 aaacagatac cgttatccaa caaatcatcc gtccgacagg gctgcttgac ccagtgatcg
     4621 aagttcgtcc aatcatggga caaatcgatg atctagtagg cgaaataaat gaacgagtcg
     4681 aaaaagacca gcgggtgttt gtaacgacat tgacgaaaaa aatggcagaa gacctcacag
     4741 attatttcaa agaattaggg atcaaagtca aatatttgca tagtgatatc aaaacgctgg
     4801 aacgaacaga aatcattcga gatcttcgat taggtgaatt tgatgtgtta gttggaatca
     4861 atttgcttcg tgaaggatta gacgtacctg aagtttccct tgttgctatc ttggatgcgg
     4921 ataaagaagg ctttttacgt agtgaacgtt ccttagtcca aacgattgga cgagctgctc
     4981 ggaatgaaga aggtaaagta atcatgtacg cagacaaagt aacagattct atgcgtctag
     5041 cgatggatga aacgtcaaga cgtcgtacga ttcagcagaa gtacaatgaa gaacatggga
     5101 tcgttccaaa aacgatcatt aaagaaatcc gagatttgat ttctattacg aaagaatcgg
     5161 aagatgatac aaaagaagcg gtacaagtct cgtacgaaga aatgacgaag gaagaaaaag
     5221 acacgttatt gatgaaatta gaaaaagaaa tgaaagatgc ggcaaaagct ttggattttg
     5281 aaacagccgc gaatgtgcga gatatgattt tagaattaaa agcttcaaag taggaggaca
     5341 acatggctaa cgataaaatt acgattcatg gtgcacgtgc ccataacttg aaaaatatag
     5401 atgtaacgat tccacgagat aaattcgtgg tcgttacagg tttgtccggt tctggaaaaa
     5461 gttccctagc atttgatacg ctgtacgctg aagggcagcg gagatacgta gagagccttt
     5521 ctgcttatgc tcgtcagttt ttaggacaaa tggataagcc ggacgtagat agcatcgacg
     5581 gattgagtcc ggcgatttct attgatcaga aaacaacaag taaaaatcct cggtcgacag
     5641 taggtactgt gacggaaatc aatgactatt tacgcttgtt gtttgcacgt gtaggacatc
     5701 cgatttgtcc caacgatcat atcgagatta ccagtcaatc agtagatcaa atggcagatc
     5761 aagtaatggc tttgcctgag cgcacaaaga tccaagtgtt ggcaccgatc gtggtaaaga
     5821 aaaaaggaca acataaaaaa atattcgaac gaatccaaaa agagggatac gtccgcgttc
     5881 gagtcgatgg tgagatctat gatttaagtg aagtgcctga gctggagaaa aacaaaaaac
     5941 atgatatcgc gatcgtcatc gaccggatca ttgtgaaaga agggattcgt tctcgcctat
     6001 ttgattcttt agaggcggcc ctgcgattag cagacggcta tgctctggtt gacgtgatcg
     6061 gtgaagaaga aatgttgttt agcgaacact atgcctgtcc ttattgcgga tttactgttg
     6121 gcgaactaga accaagactg ttctcattca atgcgccttt tggagcttgt ccagactgtg
     6181 atggtttagg tgtcaaatta gaagtcgatc aagatttagt gattccagat agaacgaaga
     6241 cacttagtca aggtgctatc gttccatgga atccaatcag ctctcagtat tatcctcaaa
     6301 tgttagcgca ggcttgtgaa agtttcggta tcgatttaga tacaccattc gaagaattac
     6361 cggaagagca tcaggagatc atcttgaatg ggacagatga tcttttccat ttccattatg
     6421 aaaatgattt tggtggcgtt cgcgatgtag aaacgacttt cgaaggggta ttgaagaata
     6481 tcgagcgtcg ttatcatgat acgaacagtg atttcacgcg tgagcaaatg cgtctttata
     6541 tgacagaatt aacttgtcag acatgtaaag gttatcgatt gaatccccaa gctctatctg
     6601 ttcaaatcaa tggaacgaat atcgggcaaa caaatgagtt gtctattcaa aaagcagtag
     6661 atttcttctc aaacttaacg ttatctgaac aagagaaagt aattgccaaa ccgattttaa
     6721 aagaagtaaa tgacagactt tcattcttgg aaaatgtggg actagactat ctaactttga
     6781 gtcgcgcttc cggaacatta tctgggggag aagcgcagcg aatcagattg gctacacaaa
     6841 ttggatcgaa tctttctgga gttttgtata ttttagatga accatcgatc ggattgcatc
     6901 aacgcgacaa cgatcgactg attggctcat taaagaaaat gcgtgatctt ggcaacaccc
     6961 tcgttgtagt cgaacatgat gaagatacga tgtgggcagc agattatttg attgacgtcg
     7021 gacctggggc cggagaacaa ggcggacaaa tcgtggctgc aggcactcct gaagaagtag
     7081 ctaatgatga aaattctttg acagggaaat atctttcagg gaaaaaatca atccctgtac
     7141 caaaagaacg ccgtaaaggt aatgggaaag caatcaagat cactggtgct tcagaaaaca
     7201 atctgaaaaa tattagtgtt gaatttccat taggagaatt tgtagcagtc acaggtgtgt
     7261 ctggttctgg aaagagtaca ttggtcaata gtatcttaaa gaaatcgtta gcgcaaaaat
     7321 taaataagaa ttctgctaag ccaggtaaat tcaagacaat ttccggctac gaaagtatcg
     7381 aaaagatcat cgatatcgat caaagcccaa tcggccggac gccgagaagt aatccagcga
     7441 cttatacaag tgtatttgat gatatccgtg ggttatttgc tcaaacgaac gaggcaaaaa
     7501 tgcggggtta taagaaaggg cgttttagtt tcaacgtaaa aggcggtcgt tgtgaagctt
     7561 gtcgcgggga tggaattatt aagatcgaaa tgcacttttt gcctgatgtc tatgttcctt
     7621 gtgaagtatg tcatggtaaa cgatataatt ctgaaacatt agaagtgcat tacaaaggaa
     7681 aaagcattgc tgatattttg gaaatgacag tagaggatgc tgtagaattc ttcaagcaca
     7741 ttccaaagat tcatcgcaaa ctgcaaacga ttgttgatgt tggcttaggt tatgtgacga
     7801 tggggcaacc agcaacgaca ttgtccggtg gtgaggcaca acggatgaaa cttgccagtg
     7861 aattgcacaa aatctctaat ggaaagaatt tctatatact agatgaacca acgacaggac
     7921 ttcatagcga tgacatcgcc cgcttgttgc atgtattaca aagattagta gatgctggta
     7981 acacagtttt agtgattgaa cacaatctag atgtaatcaa aacagcagat tatatcattg
     8041 atttaggacc agaaggtgga gaaggtggag gaacgatcct tgcgactgga acaccagaag
     8101 aaatcattaa cgtaaaagaa agttatacag gtcactattt gaaaaaaata atggtataaa
     8161 gaggatagat agctgagata gttaaaaaaa cataaaaaaa tgaatttttg cttgttttat
     8221 taatatcagt gttctagaat cattacatgg tagttctttc tacactgatt ggtatagata
     8281 aaaacttcct ataagttcaa tccgttcaaa aaagaattga gcaatcggag gactttcctt
     8341 tgattgcttt tttctttatg gtatgatatt ccttgtgtag ttggaaaaga aaaacaagag
     8401 attatgagta agtgagagat aatgatgaac agtattgaaa atgaagaatt tatccctata
     8461 ttattaggaa gcgacatgaa cgtttatggt atggcacgtt cttttaatga agcatacggc
     8521 aaaatctgcc aagcatatgc ttcggatcag ttagccccta cccgctacag taaaattgtc
     8581 aatgtagaag ttattccagg atttgataaa gaccctgtat ttatcgagac gatgcttcgt
     8641 ttggcaaaag aaagatattc tgataagagt aaaaaatatc ttttgattgc ttgtggtgat
     8701 ggatatgcag aattgatctc ccaacataaa caagagctgt cagaatattt tatctgtcca
     8761 tatattgatt attctttatt tgaaaggctg atcaataaag taagctttta tgaagtttgt
     8821 gaagaatatg atcttccgta tcctaaaaca ttgatcgtca gagaagaaat gttagtaaat
     8881 ggacatttgg aacaggaatt gccttttgaa ttcccagttg ctttgaaacc tgcaaacagt
     8941 gtggagtatc tttctgtcca atttgaagga agaaaaaaag cttttatttt agagactaga
     9001 gaagagttcg atttaatttt aggccggata tacgaagccg gttataaaag tgaaatgatt
     9061 gtacaagatt ttatcccggg tgatgatagt aatatgcgtg tgctaaatgc gtatgtagat
     9121 gaagatcatc aagttcggat gatgtgcctt ggtcatccac tattagaaga tcctacacca
     9181 gcatcgattg gtaattacgt tgtgatcatg ccagattata acgaaaaaat ctatcaaacc
     9241 attaaggcat ttttggaaaa aatcgaatat acaggctttg ccaatttcga tatgaagtat
     9301 gatcctagag atggagaata taaattattt gaaatcaatt tgagacaagg tcgtagcagt
     9361 ttctttgtga cattgaacgg cttgaatctg gctcgttttg tcacagaaga tcgagtgttc
     9421 aacaaaccct ttgttgaaac gacctatgga acgaatcaat cagataaagc aagattatgg
     9481 atgggtgtgc ccaaaaaaat ctttttagaa tatgctcggg aaaatgaaga caaaaaacta
     9541 gctgagcaaa tgatcaaaga aaatcgttac ggtactactg tattttatga aaaagaccga
     9601 tcaatcaagc gctggttgtt gatgaaatat atgttccata actatattcc aagattcaaa
     9661 aagtattttc atgtgaaaga aggctagttc atggagaatt ttttcagtat tttaggcgga
     9721 atgggcacga tggcgacaga aagttttgtt cgcttgatca accaccgaac gaaagctaca
     9781 aaagatcaag aatatttgaa ttatgtctta ttcaatcatg caacagttcc tgatcgtaca
     9841 gcttatattt tagatcgatc agaagaaaat ccaatgccgt ttctgctgga tgatattgag
     9901 aaacaaaatt tattgcgacc aaacttcatt gtgttgactt gtaacaccgc acactacttt
     9961 tttgaagagt tacaagcggc aacggatatt cctatcttgc atatgccaag agaagcagca
    10021 aatgaacttg tccgtcagca tacgacaggg agagtagcga ttctaggaac agaaggaagt
    10081 atgaaagctg gaatctatga acgcgaagtc aaaaatcttg gtttcgaaac agtgattcct
    10141 gatactgcgc ttcaagaaaa aatcaattac ctgatctatc atgagatcaa ggaatcggat
    10201 tatttgaatc aagagctcta ttacgaaatc ttagaggaag cggtagagcg cctaaactgt
    10261 gaaaaagtca tcctaggttg cacagaatta tctttgatgc atgaatttgc tgaagataat
    10321 cattacccag tgatcgatgc tcaatctatt ttagcggatc gaacgatcga acgtgcatta
    10381 gctgaaagaa gcgaggcatt ggatacagca tcggaaaagt aaaaaaattg gatttggggc
    10441 aaatatccta aaagatattt ggtccaaatc tttttgtaaa agaaaaagaa aactggtaat
    10501 gatacgcccg ggatgatata attttattat gcacaatgtg agaggggttc gtaaatatga
    10561 cagataattt gcaattagtc atcataacag gtatgagtgg tgcagggaaa acagtagcta
    10621 tccaaagttt tgaagatatg ggctattttt gtatcgataa tatgccgcct cgattgattc
    10681 caaaattctg ggaattgatc aaagaatcag gtaaagtcac aaaaatggct ttaggagtgg
    10741 atttgcgttc tcgttccttc tttgaagaaa tccaaaacat gctgatcgaa atcgaaaata
    10801 cgaacttcat cgacacttct atcttatttt tagatgcttc agacgaagaa ctagtggctc
    10861 gctacaaaga aacaagacga gcacatccaa tggcgatgga cggtttgatc actgaaggga
    10921 tacgtaaaga acgggcgata ctagacgact tgaaagccca agcttccgtg atcattgata
    10981 cgacaacatt gacaccacga caattacgtg aaaaaatcaa tgagtcattc aaatcaaagg
    11041 gagaccaagg atttcggatc gaatttgtat catttggttt taaatacggg ttgccgatcg
    11101 attcggatat tgtgatggat gtgcgttttt tgcccaaccc tcattatatc ccggaactaa
    11161 gaccattgac cggaaaagat cagccagtct atgattatgt gatgtccttt gatgaaacgc
    11221 aatcattcta ccataaattt ttggatcttc tggaaactgt attgcctgga tacattaaag
    11281 aaggaaaaag cagcttgaca gttgcaatcg gctgtacagg cggtcaacac cgttctgtgg
    11341 cattgactga acgtttggca aatgctttag ggaaaaacta taaagtcaat gtgacacatc
    11401 gggacaagga taaaaggaaa gagacggtga accggtcgtg aaaatgaaaa cctaccggat
    11461 aagaaaacca aagatcgtag tggtcggcgg tggcactggt ctgccagtta tcctgaaaag
    11521 tttacgaaat caaggagcag atatcacagc agtagtgact gttgcagatg atggtggaag
    11581 cagcggtgca atcagagaat cgatcgctat ggctcctcct ggtgacttga gaaatgtctt
    11641 agtggcgtta tcggatatgc cacaattcta tgaagatatt ttccaatacc gtttcaaaaa
    11701 ggaagatcag tttttagcca atcatacgat cggtaatctg atcattgccg cagtttccga
    11761 gatgcgggga agcacttatg aggcgattca attattagca aaaatgatgc atgtagatgg
    11821 acatgtttat ccttcttcag aaactccttt gaccttgcat gctgttttta aagacggaac
    11881 gactgcagta ggagaatcta aaatcgcgat caaccgtaaa accattgacc gtgtgtatgt
    11941 ctgcaacaca aatgataaaa gtgaagccaa agcggctcga aaagtcgttt cagcaattat
    12001 ggacgccgac atggtcgttt tgggacccgg aagtttattc accagtatcc ttccaaattt
    12061 agtgatacca gaaatcggcg aagcaatgct caagacaact gctgaaaccg tctatatctg
    12121 taatatcatg acccaaaaag gggaaacgga acatttcaca gatgccgatc atatacgtgt
    12181 t
//
LOCUS       DACEXQ010000065        12123 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.065, whole genome shotgun sequence.
ACCESSION   DACEXQ010000065 DACEXQ010000000
VERSION     DACEXQ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12123
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.065"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            6..305
                     /locus_tag="IXH04_001270"
     CDS             6..305
                     /locus_tag="IXH04_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1851890.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="IXH04_001271"
     CDS             378..1739
                     /locus_tag="IXH04_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAQ1851891.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="IXH04_001272"
     CDS             1761..2021
                     /locus_tag="IXH04_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851892.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="IXH04_001273"
     CDS             2035..2787
                     /locus_tag="IXH04_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAQ1851893.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="IXH04_001274"
     CDS             complement(2881..3486)
                     /locus_tag="IXH04_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1851894.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="IXH04_001275"
     CDS             complement(3539..4513)
                     /locus_tag="IXH04_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAQ1851895.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5670)
                     /locus_tag="IXH04_001276"
     CDS             complement(4891..5670)
                     /locus_tag="IXH04_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1851896.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IXH04_001277"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IXH04_001277"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ1851897.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="IXH04_001278"
     CDS             7161..8558
                     /locus_tag="IXH04_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1851898.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="IXH04_001279"
     CDS             8639..9079
                     /locus_tag="IXH04_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ1851899.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="IXH04_001280"
     CDS             9227..9553
                     /locus_tag="IXH04_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1851900.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="IXH04_001281"
     CDS             9554..10303
                     /locus_tag="IXH04_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851901.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="IXH04_001282"
     CDS             complement(10383..10838)
                     /locus_tag="IXH04_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ1851902.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="IXH04_001283"
     CDS             complement(11265..11540)
                     /locus_tag="IXH04_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851903.1"
                     /translation="MKLEDKKVILKKIKRLPEQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..>12123
                     /locus_tag="IXH04_001284"
     CDS             11834..>12123
                     /locus_tag="IXH04_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAQ1851904.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNY"
BASE COUNT         3857 a         2082 c         2462 g         3722 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttgctct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 cca
//
LOCUS       DACEXQ010000066        12015 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.066, whole genome shotgun sequence.
ACCESSION   DACEXQ010000066 DACEXQ010000000
VERSION     DACEXQ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12015
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.066"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(238..1488)
                     /locus_tag="IXH04_001285"
     CDS             complement(238..1488)
                     /locus_tag="IXH04_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HAQ1851905.1"
                     /translation="MKKKYNIAVAGTGYVGLSIATLLAQHHQVTAVDIIPEKIELINK
                     RKSPIQDEYIEKYLTEKELDLTATLDARSAYKSADFVVIATPTNYDSRTQHFDTSAVE
                     SVIEVVMDVNPDTIMVIKSTIPVGYTESVRETTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMTDERLLNAAHVFATLLQKSAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPGNLIEAIVESNRTRKDFIADRVLQMAGYYNYAEYNQYDVNRENPVLIGIYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPALKDGETFFGSKVINDLEQFKAMCDSII
                     ANRYDTCLDDIKEKVYTRDIFQRD"
     gene            complement(1866..3317)
                     /locus_tag="IXH04_001286"
     CDS             complement(1866..3317)
                     /locus_tag="IXH04_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ1851906.1"
                     /translation="MYKKSSRGWVKHIDFIILDLLCIQLAFYLSYVLRQVDWNPYVIP
                     MYQNMAVFIELADLLVIFMFEAYRGVLKRGYYIEFASSVKQAIMLVLLGSLYLISVQG
                     GNDYSRVVLFLMGGLYVVITYITRLLWKHYLKHKMKNEGNKSLIIVTSSSIAEVVIRN
                     VKEHNYKMYKINGVIIIDKSMIGEDVEGVTVVSDSKNAADYLCQGWVDEVFIILNKST
                     SCMKEFIERCSEMGLTVHLNLAKVSDSALGKQSIGRIGEYTVLTTSINVMTTQQAFIK
                     RTIDILAGIVGCIATGIIFIFIAPLIYINSPGPIFFAQERIGQNGKPFKMYKFRSMYM
                     DAEERKVELMKENKMSNNLMFKMEFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FFNVLTGKMSLIGFRPCLKSEYEEYDYHHRARIAIKPGITGMWQVSGRSDITDFEEVA
                     KLDTEYIKNWSMGLDFKILFKTVKTVLQRGGSM"
     gene            complement(3638..4402)
                     /locus_tag="IXH04_001287"
     CDS             complement(3638..4402)
                     /locus_tag="IXH04_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAQ1851907.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNVHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(4430..5128)
                     /locus_tag="IXH04_001288"
     CDS             complement(4430..5128)
                     /locus_tag="IXH04_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAQ1851908.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILSEARQLFDV
                     VIFDMPPVVAVTDAQIMSLKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(5140..5919)
                     /locus_tag="IXH04_001289"
     CDS             complement(5140..5919)
                     /locus_tag="IXH04_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAQ1851909.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(5935..6879)
                     /locus_tag="IXH04_001290"
     CDS             complement(5935..6879)
                     /locus_tag="IXH04_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1851910.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(6925..7704)
                     /locus_tag="IXH04_001291"
     CDS             complement(6925..7704)
                     /locus_tag="IXH04_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851911.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQYHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKIRAAYATKAQTVAQQMGYSNISY"
     gene            complement(8084..10159)
                     /locus_tag="IXH04_001292"
     CDS             complement(8084..10159)
                     /locus_tag="IXH04_001292"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAQ1851912.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLAGVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLEDVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(10161..11078)
                     /gene="glyQ"
                     /locus_tag="IXH04_001293"
     CDS             complement(10161..11078)
                     /gene="glyQ"
                     /locus_tag="IXH04_001293"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAQ1851913.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPETVKEAE"
     gene            complement(11464..>12015)
                     /gene="recO"
                     /locus_tag="IXH04_001294"
     CDS             complement(11464..>12015)
                     /gene="recO"
                     /locus_tag="IXH04_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAQ1851914.1"
                     /translation="LSGYGTYLLNLVDAAIEDHQYDPHLFQFTQQALQRMDQGDDAEI
                     ITNIFEVQILQRFGIAPIWTHCVVCGETKGKFDYSSKYGGVICERHWSMDDHRYHADP
                     RAVYFVRMFSAISYDKISGIHLKEETKQAIRELIDQLYDEYVGIHLKSKKFINQMKSW
                     AHVLKTSEKDMSEKEKETPEKEE"
BASE COUNT         3317 a         2539 c         2048 g         4111 t
ORIGIN      
        1 ataataattg atttgaataa attaataagt atagtacaca aaaaaaactt ttcttttcta
       61 tttacttgtc tcatgtagta cgttttttcg ccttttgact cagctgaata ttctgtctgt
      121 tctgttataa caaaactctc atacttctcc atcagtggtt ttaacatcat taactgttca
      181 taatgccccc cagaggaagc cgcaaaacat atctttttgt tcatattaac ctctctatca
      241 gtcacgctga aaaatatctc tagtgtaaac tttttcttta atatcatcca agcaggtatc
      301 atatcgattg gcaataatag aatcacacat cgccttaaac tgctctaaat cgttgataac
      361 cttacttccg aaaaaagtct ctccatcttt cagtgcaggc tcataaatca ccacagtcgc
      421 acctttagct tttacgcgct tcataactcc ctggattgaa ctctgacgaa aattatcact
      481 gttgctcttc atggtcagcc ggtatatacc gattaaaact ggattttccc tgttgacatc
      541 atactgatta tattcagcgt agttgtaata gcccgccatc tgaagaacac gatctgcgat
      601 gaaatcctta cgggtacgat tagattcaac gatagcctca atcaaatttc caggaacatc
      661 tgcatagttt gccaacaact gcttggtatc ttttggcaag caataacctc catatccaaa
      721 ggatggattg ttgtagtgag ttccaatacg aggatcgaga caaacaccat tgattatctg
      781 ctgagtattc agccccttca tttcagcata agtgtctaat tcattgaaat agctgactcg
      841 cagtgctaaa taggtatttg caaacagctt tactgcttcg gcttcagtga atcccataaa
      901 aagagtatcc acgttctcct taattgcact tttctgcaga agtgttgcaa aaacatgagc
      961 agcatttaac aagcgctcat cagtcatatc agtaccaaca ataatacgag atggatacag
     1021 attatcatat aaagccttgc tctcgcgcag aaactctgga ctaaaaataa tgttcttact
     1081 ccccgttgtc tcacggactg attcagtgta gccaacagga attgtagact tgatgaccat
     1141 gatggtatca ggattcacgt ccatcacaac ctcaataact gactcaacag cagaagtatc
     1201 gaaatgctga gtacgactgt catagttagt cggtgtagca ataacaacga aatctgcgga
     1261 tttataagcg ctccttgcat ccaaagtagc agttaagtca agttcttttt ctgtaagata
     1321 cttttcaata tattcatcct gaattggaga cttccgttta ttaatcagtt caatcttttc
     1381 agggataatg tctaccgcgg ttacctggtg atgctgcgcc aatagtgtgg cgatactcaa
     1441 accaacataa ccggtgccgg ctactgcgat gttgtacttt ttcttcacaa tatctcctct
     1501 ttcctctctt aagtatggag aatgttactc ctacggctat tcatcatgtc aaaataacac
     1561 agcttagaat gttgataaaa taattttcca gaatgtgata aaatctcttt catttataaa
     1621 ccacacatct aattttatct attctccaca agacatgtag agtattcttt aataacaaga
     1681 tacgacctta ctaaatactt caaaaaatta tcatatttaa ttcataaata taaatcttca
     1741 ttttaaatgt atgcccaaaa atttatctgt aatgcgtcat caaaatctag attccaaatc
     1801 tttatcaatt tttatccact cattctacca ctttagtaat attttctatt tataacactt
     1861 tcgtcctaca ttgatccccc cctctgaagt acagttttta ctgtcttaaa taaaatttta
     1921 aaatctaacc ccatactcca attttttata tattccgtgt caagttttgc tacttcttca
     1981 aaatcagtaa tatcacttct accacttacc tgccacattc cagtaattcc tggcttgata
     2041 gcaattcttg cacgatgatg ataatcatat tcttcatact ctgatttaag gcatggtcga
     2101 aatcctataa ggctcatctt tcctgttaga acgttgaaaa attgagggaa ttcatctaaa
     2161 gacgtggatc taataaattg accgattcct gtcttttttg ttccatctgg aagtatttta
     2221 tttccgataa ttcttggatc aaactccatc ttaaacataa gattattgct catcttattt
     2281 tctttcatta attctacttt tctttcttct gcatccatat acatacttct aaacttatac
     2341 atcttgaacg gtttaccatt ctgacctata cgttcttgtg caaagaaaat tgggcctggt
     2401 gaattaatat aaataagtgg agcaataaag ataaaaataa ttcccgttgc aatacatcca
     2461 acaatacctg caagaatatc aattgttcgt tttataaatg cctgttgagt tgtcataaca
     2521 ttgatacttg ttgtaagtac tgtatattct cctatacgac caatagattg ttttcccaaa
     2581 gcactatctg atacttttgc aagattcaaa tgtacagtaa ggcccatttc agagcatcgt
     2641 tcaataaatt ctttcataca cgaagtactt ttattcaaaa taataaagac ttcatctacc
     2701 catccctgac ataagtaatc cgctgcattt ttggaatcag aaacaactgt cactccctca
     2761 acatcttcac caatcatgct tttatctatg ataatgacac catttatttt atacatttta
     2821 tagttatgct ctttcacatt acgaataact acttcagcaa tactactaga tgtaactata
     2881 ataagtgatt tgttcccctc atttttcatt ttatgtttca agtaatgttt ccataagagg
     2941 cgagttatat atgtaattac aacgtataaa cctcccatca agaaaagaac gactctagaa
     3001 taatcatttc caccctgaac actaatgaga tacaaacttc ctaacagcac caacataatc
     3061 gcttgcttta cagaagaagc aaattctata tagtaccctc tttttaatac acctctatat
     3121 gcttcaaaca tgaaaataac taaaagatct gccagttcaa tgaagactgc catgttttga
     3181 tacattggaa tcacataagg attccaatct acctggcgta agacatacga tagataaaaa
     3241 gcaagctgga tacacaataa atctaaaata ataaaatcaa tatgtttaac ccatcccctt
     3301 gaacttttct tgtacattcc tttttactcc cctcttttta ataaaataaa ttgtattact
     3361 tgttttctct cctaaatatc tttctgaagg attattgact cgacaaattg gcttgtggat
     3421 attcttatat atagaacatc cgtaagccaa cttaaaactc cttaacaaaa tttttttact
     3481 catattgtag atcaaaacaa gtatccaaga aatcatattt tatttctttt ttctgcttac
     3541 tcaacaacag ctaaaagaaa aatcatcata aacaaattca actaagccaa cgatcccctt
     3601 gtgaaatcaa gttttttctt gatttacgaa taaagagcta aaataaacca aacttcttct
     3661 tttttacctc ttcatagact ggataactga tttcatcccc gttgatcaga tcctttgcga
     3721 cttgtttcat ctgtactact ttttccttac caaagtcctt tgcaatctgc tcatagcact
     3781 cttttaagta aaaggtgcgc ttagagaccc catgggcatc agaagcagcc atctgcacga
     3841 gattatgttt gaccatcgtt ttggctgttt tctggatgtc tttgccaaat accccaacat
     3901 aacttggcgc agtgagttgg gccaaacatc ccatatctag gaacggaagc aaatgatttg
     3961 ggtcttcgcg aaaatgcacg ttgcgttctg gatggacgat tactggtgtc tttcctagtt
     4021 ctaacaactc aaaaaaaagc cgttctgcat ataacggcac ttccagtgtc ggaaattcga
     4081 tcagaagata tgtatcatct aaatctgtaa ataatatttc atctcggtgg atgtcttcga
     4141 tgagcgttcc ggtaatacgt acttcttgtc cttccaataa cgtcaaaggc aactgtcttt
     4201 tctctaattc ggcttgtaac gaagcgacta gagaaatgac ttgtgatttt tcattgctgt
     4261 attttccatt attgtggtgt ggtgtgcata aaatatgcgt gatgccttgc tgaatcgctt
     4321 tttcagccat tgcaatactt gcttctaagt tttctgcccc gtcatcgatt cctggtagga
     4381 tatggcaatg tagatcgatc atttctcttg tctcctctct tcttattcct taatttccgt
     4441 agtaataacc agcatctttg ctatgttctg ctccgttgta cacaacacct aagactcgtg
     4501 cttgtaccat ctccagcagt tcttttgctt tagtcaagga ttctttacgt gacatatttt
     4561 cacgtacgac caataaagtg ccatccgttt ttaaagacat gatctgtgca tctgttacgg
     4621 ctactactgg tggcatatcg aatatcacca cgtcaaataa ctgtcttgct tcggatagga
     4681 tctgatccat tcttggcgag cttaacaatt ctgatgggtt gggtggctta ggtccactag
     4741 gcaaaatcga caagttctcg actggcgttc gttggatcac atctgctaca cttcctgatg
     4801 aactaagtgc tgtacttaat ccactggcat tgttcagctg gaatgtctta tagacgacgg
     4861 gtttacgtaa atctgcatcg actagtagaa ctcgttgacc tgatttagca aaaacgactg
     4921 caatgtttgc tgcagtggtg gatttccctt ctccaggacc tgatgaagtc acgacaatcg
     4981 ttttgatttg ctgaccagct gcggaggcaa actggatatt cgtccggatc gtacgatatt
     5041 gttcggcgat tggtgaagat gggtttgtta atgtgatcaa actgactgca tgagtttgtg
     5101 ctgctttttg tttctgtgtt cgtgccattt ttcttcctcc tagactcttg agcggcttct
     5161 gcgtgatggg gctgttttat cgtttgtagc ttttggtcgt gcaggagctg ttcctttttt
     5221 acggatacca gcgttcaatt ctttcgctga catttctgga actgttccta agattgggaa
     5281 tcctaatgta tctgtcacat atttttcatc tttcactgtc cgatcgagta attccaataa
     5341 gaatgccaga cctacaccta ccatcattcc taacattaag ccagtcatta aattcaattt
     5401 attatttggc gaaacaggtg tcgtattggc aactgcacct gaaatgatcg agattttatc
     5461 aacattcatc acatcttttg cgttctcttg gaaaacttgt gaagttgtgt tagcgatctg
     5521 ttgagcagcc actgcattcg tacttattgc ctgaatcgag aacatttgtg aattttctgc
     5581 ctgattaact gagatggcat ctttgatttg ttcagcagtc attttcacat tgtcttctgt
     5641 ttctaaacga tctttgactt cattgagcac taaatcactg acgatcatat ctttatacgt
     5701 attgatcatt tgcaggttcg tattgacatc attgacattt gcagtgtctg attgtggcag
     5761 tgtcacgatc aattgtgctt gtgaactgta ttttggtgtc aagatgaaaa atgttacgat
     5821 tcctgagatg gccaagccag caaacattga gatgatgatt attaccaagt gtttgcgtaa
     5881 tgtttcaaaa atttctttca agctgattgt ctcttccatt ttttctccac tttcctattt
     5941 attctctaga ttcatttgat cttttaattc ttttttcaca cgatcaagtt cttgatcact
     6001 gactttttga taggagatat tatcttgcat gaagccttct ccttgcagct gatcttgctt
     6061 aatcgtacca aatgcctccc gataatctaa agcaattttt ttcatttcat caaagttcat
     6121 gtctgttttc atattcgact ctagagcatt caaaacggat tgatagttgc tgacaccgtt
     6181 tagactcaat acttttttcg ctacgccttc tacgatcttt ctttggcgcc cttgtctacc
     6241 gtaatcacca tttgggtctt cataacgcat acgagaataa gataatgcct gatccccgtt
     6301 caatgtaatt ttaccgattg gaaagtcata gccatcttgt gagaaagtga ggtcattatt
     6361 gacttcgata ccaccaactg catcaacgag ttcctttagt cctttcatat tgatagagac
     6421 atagtgatcg attgggatgt ctacatattt ttgtacggta tccatcgcca tggcagctcc
     6481 gccgaatgcg taagcgtggt tgatcttgtc ttttgtgtca cgacctacga tgtcgacata
     6541 cgtatctcga ggaatgctga cgatcgttgt ctgtttgtct tcaggactga ctgtggcaac
     6601 catcatcgta tctgatcttc cttgatcgac ccgacctaaa tctccagtat caatccccat
     6661 caatagaaca gagaaagatt cttgtttgtc cagatcaact ggtttcgttc gttctccttg
     6721 ggttcgttcc actgattcgt aagttttctc aatagaatgt gagagatcca gatataattt
     6781 tgctcctact cctacaattg ctaatactaa tacgataaga actgctaaaa taatcaaaaa
     6841 tatttttttc cctcttgatt ttcttcttcg tcttgccata gtttcctccg tttgctaacc
     6901 ttgcttgaat caacgtttta actgtcaata gcttatattg ctatagccca tttgctgagc
     6961 aacagtttgt gcttttgttg cgtatgcggc tcttattttt tcattccgga tctgctctat
     7021 ttgactgagc gccgtcaaat attgatcgta agtagcggta gctgttactt gaccgtcttt
     7081 cagcatcgta tcgaatagct gatctagttg cgtgacttgt gcaatttggg tatcagcgaa
     7141 tcctagatat tgcataacga ctgttttcca agcactatcc ttaaaaaagc tcatattttc
     7201 acgtacatgg gcgacatctg cacttgccaa cttttctttg atgatgacat catccacagc
     7261 ttgctgccag tttgatacgt ttttagtaaa taatttattg acagccgtct ggatcttcag
     7321 ttttgattct gcatcctcta actgcgttaa gactgctttc ttttcttctt ggatcgcagc
     7381 agcttcttta ggtaattctg attccttgat gtcataatca tctgccgaaa gtttgataga
     7441 agatagttta tgtgacaaat ttgaaactgt ttcttctgtt atttcaggat ttaggaaaat
     7501 tttttctttt gtactgtaca gcttatctac ttcttttgat aaagaggtca cggtgtcttt
     7561 ttgattggtg atggcagatt ctgcgtaatt gatccgttga tgatattgcc gctgattatt
     7621 gagatggtat ccaaacaagc aaatcacaat agcgcctgtg cctaccgcca cagtagccaa
     7681 taacttgacg aacggctttt ccatagattc actcctaaat attgatgata gataagtaaa
     7741 gatgtgattc attcacatca gaatgagcgc tttttatttt aaaagcataa ttaaactaat
     7801 atattcatag aaatatttat aaacaggttt tccctatata atcataccac atacagtttt
     7861 tttgactagg gaaattccta atttaaaagt aaataaaaca gtaacaagat tataattaat
     7921 cgttttcatt tttatttcct cacttttcat gagcgttttg aaaattcgaa caaagaataa
     7981 aataacaaaa attattaaaa tagaattaat aacagctatt tataacaaaa aaaacagaga
     8041 cacagcacgt ttcgtgcatg ctctctgttt ttcaaacaaa agactatttt gtgatgattt
     8101 gatccaagct tgctaatgac aaagccatgg cagctatttg acttagttgt gttagtcggt
     8161 tcttacgtac tgctggttca tcagccatga ccatcgtttg atcaaaataa ttttcgatca
     8221 gtggacgaag agcgacaagc tggtcgtagt tttcgtggat cgtttggtca gcaaaacttg
     8281 ctttcattgc ttctactgct ttgtgtaatt ctttctctgc gtcgttttcg aacagctcag
     8341 ggtctacttc tgattgtttt tcaagctgcg cttttttcgc taagttcacg acacgtgtca
     8401 aagcttccat tgaaggtttg aagtcttcat ctgcaagatg ttgtttgaac aattgtgctg
     8461 ctgcaaatac ttttgtcaga tctttttgtt ccgcattcaa aacagcatcg atgacatcat
     8521 gacgaatatt cttcgttgtc aataattggc gcagacggcc tttgataaag tcgatcactt
     8581 gttgttgccc ttctgataaa ccaatgccga aacgatccgt atctttactg ataacatcgc
     8641 taatcgtttc ttgcagaaca gacaaaggaa aggcccattt tttcgattcg atgatccgaa
     8701 cgacaccgta tgcttggcga cgtaaggcat aaggatcgtt tgacccgctt ggaatcatcc
     8761 caactgcaaa gaaagaaaag atgctatcta atttatctgc aatcgctaaa acagaaccta
     8821 ctgtagactg aggtagctct ccttcactgg atataggcat gtaatgctca cgaatcgctg
     8881 tcgctacagc aggtttctcg cctttcagaa gagcatattt ttcgcccatg atcccttgta
     8941 gttcaggaaa ttcccctacc atgttggtta ctagatcaaa tttatagatt tcagcagctc
     9001 gttgcaaatc catcaattct tcctcagata gtcctacttg ttcgccaatg atacgggcaa
     9061 tgacttgtac ccgttgcatt ttctcataga tactaccgat tttttcatgg aaagtaacag
     9121 tttttagttt ttcaacgtat tggtcgattg aagctttctt gtcttcatta taaaagaatt
     9181 ccgcatcttc taaacgagcc gtcaatactt tttgattgcc tcgaaccaca tcttccaatt
     9241 ttacccgatc accattacga gcagaaataa aatgaggaag cagcatacct tgatcattcc
     9301 gtacctcgaa ataacgttga tgttctttca tagaagtgac tagtacttct tcaggaacag
     9361 aaagatattt ttcttcgaag tcgccagaaa agacagttgg atattcgact aaattgttga
     9421 cttcttctag taaatcttcg tctagatcaa taacccagtg atttttttct gctaattcgt
     9481 ttgcttgtgt aacgatcatt tcttgtcttt tttgcggaga cgcaatgacg aattgttcat
     9541 tcaatttttc ttcatattct tttgcatgcg caaatgtcac atcttcgcct aagaaacgat
     9601 gacctcgaga ggctctgcct gttttgacat ccaagatttc gaatgggatt acttcgtcat
     9661 ctaacaatgc cacgatccag tgaatagggc ggatatattc aaaatcgtat tctgcccaat
     9721 gcatcgttac agggaaagtc aaacttttga tcacccctgc taagcctgtc aatacttctt
     9781 ttgcttcctt accatgagtg tatttcgtta catagacata ttccacacct tttaattctc
     9841 taaaagtgat agcgtctgtc gtcagacctt gtccgcggac aaatccttcc gctgctttcg
     9901 tccagtttcc atctgcgtcc aaagcaattt ttttggctgg tcctttgact tcttcttcac
     9961 tgtccgcttg tcgttctgga atatttgtta ctttgattgc taaacggcgc ggtgtggaaa
    10021 atgtttcgat cgtatcataa gtcaattggt tttcatctaa aaattttgct gttttttctt
    10081 ctaattgttt catgcttggt gtgacaacat gggcaggcat ttcttccagc ccgatttcaa
    10141 gtaataagtc ttttgccatc ttattcagcc tccttcacgg tttctggatt tgctgtgttt
    10201 ttattcaaca aagggaatcc taatttttcg cgttctgcca caaagatttt ggctacagca
    10261 cgagccatgt tgcggatgcg agaaagataa ccggcacgct ctgttacgga tactgcaccg
    10321 cgggcgtcta ataagttgaa tgtatggcta catttcaaga tataatcata cgccggatga
    10381 acaagatttt catcgataca gcgtttagct tctttttcaa aacgatcaaa gttgcttagt
    10441 agcaagtctt ggtcgcttac ttcaaaagaa tatttggaat gttcatattc tggttgtttg
    10501 aagatctcgc catatttcac gccatcagcc cattctaaat catagacact ttcaacttct
    10561 tggatataag aagcaagacg ttctaatcca taagtgatct cagaagttac tggtttacaa
    10621 ggcagtccac caacttgctg gaagtacgtg aattgtgtga tttccattcc gtcaagccat
    10681 acttcccaac ccacaccggc acagcccatg gatgggttct cccagttgtc ttcaacaaaa
    10741 cggatgtcgt gttctaatgg atcgattcct aaaaggcgca agctttctaa ataaagctct
    10801 tgaatattct caggtgaagg tttcatcacc acttggaatt gatgatgctg atacaaacgg
    10861 tttgggtttt caccataacg tccatcagct ggtcggcgag atggctctac gtaagcagca
    10921 ttccatggtt ctggtccgat tgcccgtaaa aaggtatatg ggctcatcgt acctgctcct
    10981 ttttctgtgt cataagcttg catcagcatg cagccgtttg atgaccaaaa tttttgtaat
    11041 gttaaaatca tatcttggac ggtcaatttt ttactcatct gttttgcccc tttcaatttt
    11101 aaagcgttgc tgtcaaataa gaatacagga aagaaaacga caaaaaatcc ctatgtctga
    11161 ttacagacat agggacgaat aattcgcggt tccaccctac ttccgaataa agattcggca
    11221 gcttcattta tcgtactcgc aagtgcctct tcaaaaatcg tttgggtccg gctcccactc
    11281 tcccgaactc gctaaaccac gtgtttttta ctgatcttgg tcaatgtacg tattcatttt
    11341 gcagttttct tgtttctata atagatccta tcactgaacg atctatttaa tctatggtta
    11401 ataatagacg gaactccctg tgtttgtcaa atggttttga ttttttgttg tatgttttat
    11461 ttactattct tctttttcag gtgtctcttt ttctttttca gacatgtctt tctctgacgt
    11521 ctttaatacg tgtgcccaag acttcatttg attgatgaac tttttgcttt tcaaatggat
    11581 accgacatat tcatcataca gctggtcgat gagttcacgg attgcttgtt tggtttcttc
    11641 tttcagatgg attccactaa ttttatcata agaaatagct gaaaacatcc gaacgaaata
    11701 aacagctcga ggatctgcat gataacggtg atcgtccatc gaccaatgtc tttcgcatat
    11761 cacaccacca tatttggaag aatagtcaaa ttttcctttt gtttccccgc atacgacgca
    11821 gtgtgtccag attggtgcaa ttccgaagcg ttgcagtatt tggacttcaa aaatattcgt
    11881 gatgatttct gcatcgtctc cttggtccat ccgttgcagt gcttgttgcg taaactggaa
    11941 caaatgcggg tcgtactgat gatcttcgat tgccgcatct actagattca ataaatatgt
    12001 gccatagccg ctcaa
//
LOCUS       DACEXQ010000067        11902 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.067, whole genome shotgun sequence.
ACCESSION   DACEXQ010000067 DACEXQ010000000
VERSION     DACEXQ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.067"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            257..532
                     /locus_tag="IXH04_001295"
     CDS             257..532
                     /locus_tag="IXH04_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851915.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            545..2623
                     /locus_tag="IXH04_001296"
     CDS             545..2623
                     /locus_tag="IXH04_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ1851916.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            2783..4669
                     /locus_tag="IXH04_001297"
     CDS             2783..4669
                     /locus_tag="IXH04_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1851917.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4681..5628
                     /locus_tag="IXH04_001298"
     CDS             4681..5628
                     /locus_tag="IXH04_001298"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ1851918.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5648..6175
                     /locus_tag="IXH04_001299"
     CDS             5648..6175
                     /locus_tag="IXH04_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1851919.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6238..6891)
                     /locus_tag="IXH04_001300"
     CDS             complement(6238..6891)
                     /locus_tag="IXH04_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ1851920.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7023..7865
                     /locus_tag="IXH04_001301"
     CDS             7023..7865
                     /locus_tag="IXH04_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1851921.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8023..8919
                     /locus_tag="IXH04_001302"
     CDS             8023..8919
                     /locus_tag="IXH04_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ1851922.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8922..9635
                     /locus_tag="IXH04_001303"
     CDS             8922..9635
                     /locus_tag="IXH04_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAQ1851923.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9655..10173
                     /gene="msrA"
                     /locus_tag="IXH04_001304"
     CDS             9655..10173
                     /gene="msrA"
                     /locus_tag="IXH04_001304"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ1851924.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10170..10397
                     /locus_tag="IXH04_001305"
     CDS             10170..10397
                     /locus_tag="IXH04_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAQ1851925.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10549..11100
                     /gene="lepB"
                     /locus_tag="IXH04_001306"
     CDS             10549..11100
                     /gene="lepB"
                     /locus_tag="IXH04_001306"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ1851926.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11183..11725)
                     /locus_tag="IXH04_001307"
     CDS             complement(11183..11725)
                     /locus_tag="IXH04_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ1851927.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
BASE COUNT         4161 a         2015 c         2427 g         3299 t
ORIGIN      
        1 tttttgtcgc agccccattt ttttgttgtg ataaaagaac aagaaaggca ggaatgggaa
       61 agtaaacatc cttttgtcag aaacttttat ctttcttaag ttttacttga tatctgatac
      121 tatttggaat agaatgaggc gttgaaaaat ttatggaaat ttctaactag caataaacga
      181 tgaaacaatg aaatattttt catccaaaaa gaactttgct ataatgatgg aagtttgtgt
      241 ataggagatc tggaaaatga gagagagttt tgaacaacaa aaaaaactgt tgcatgatcg
      301 ctatggcgcc ctttcgatgg atgaccgccg tcaaatactt tgtaagttgc gtaaaagaaa
      361 tatattgatg tatcgtcagt tggaacgtct gaaacatgat ctgcttcgtt tggagtcaaa
      421 acgtgtacag tgtgaattag aggggaacca aacacaggtt gaagtagtcg aaaccaaaat
      481 tttaaagaaa aaagaacaat ttttaaaaat gcttacacaa aataaaaaat aaaggggatg
      541 gacgatggaa tttgctgagt tagttattgt atttgcagta acgatcacct tatcgaacat
      601 agctagccgc attttgccaa tgattccagc tccactgatc caaattttcc ttggagtggt
      661 attaggtctg acagagtggg gacaatcaat cgattttgaa cctgaattgt tcttagttat
      721 gattattgca ccattgttat ttagagaagg agaaaaagca gatatttctt cgattctcaa
      781 aaatttcggg acaattttgt tcttggcatt tggaggagtc attttgaccc ttgtcggggt
      841 aggtgcgacg ttatcattcc tgttgccaag tgtcccactt gctgcctgct ttgcttttgg
      901 agcagcgtta ggtccgacag atgcggtagc agtcagttct ctttctggac gagtgaatat
      961 ccctaaaaag gcgatgcata ttttggaagg agaaggtctt ttgaatgatg catctggtgt
     1021 tactgccttc cagtttgctc ttggtgcgct gatcaccgga tctttttcag cagtaaacgc
     1081 ggggatgagt ctagtagttt ctagtattgg gggagctttg atcggctttt tgcttgtctg
     1141 gttcaaacaa aaaataattc atttgatcga aaaagcatca gcacaagatg tcacagggta
     1201 tctgctgatc gaacttttgc ttccttttct agcctatgta ttggcagagg catttgatgt
     1261 atctggaatc attgctgcag tggcagcagg tattctccaa gcttctggtt ttaggaaaat
     1321 ctctgtgttt gatgcagaat tgtcgagctt gtcccacagt acatggacaa ctattgcttt
     1381 tacactgaat gcattagtat ttatattttt aggaatcgaa ttgacgcaag tgttctcgcc
     1441 agtatgggga gacggtttat atccaaacgg tcttttatta gcgattatcg tattaatctc
     1501 tgtcatgctt tttgtgatca gattcatttc tatcagtctt ttttatgtat tcaaagatgg
     1561 cagtaaaaag ttcaaaaaac agttgaacga gatcttgatc ttaacatttg gcggtgtaaa
     1621 gggaacagtc agtttggcta cgatttttat tttgccattt tcaatcaata atatgatgtt
     1681 ttaccagcgg tctttgcttt tgtttctgac ggcgggtgtc atcttagtga ctttggtcat
     1741 agggatcatt gtcttgccta tgctgacgga aacagaggaa gcgaaaagca cggatttgaa
     1801 tgctttgatg attttagaag aggtcgttga agttttgcga aaagaaataa aggaaattga
     1861 tcgtaataca aaagaatttt tagccactga agcagtgata gaaaattatc aagaacggat
     1921 acgtgatctg tacttggaag atttaaccga tgatgaaaag caagaagtac aagaaataca
     1981 agcactgatt ttgtcgatcg aacgggatgg attggatgaa agttatcgtt cgggcaagct
     2041 ttcttctaat ggttatcgtt tttactcccg ttttctttct cgattcgaac attctatcac
     2101 aagccagatt ttgtctttta tcggcttttg gttcattgtt gtccgtaggt taatgcgaat
     2161 cgttctccat ccgaaaatgt tttggcaaag aagatatgct gatcgtcaga catttattag
     2221 tgatgaagat atccaagaaa tcagagagac atatcaaaaa aatactcagt tgattattga
     2281 aagtttagat aacttgactg atatttatga tgaaacgatg gttcaatttt ttatccagca
     2341 acgcaaactt gaaggaataa aaatgaactc ggggaatttg atttcttcgt ggatgatcca
     2401 gcaagatagt ctgtttacga aaaaaatgct aagaggttat tatcttgaaa gaaaaatcat
     2461 tgatgaatac gaagttgctg aaaaaatcac gacgttttct gcgaacagct atcgcaggaa
     2521 tatcaatttg ttagaatctt atacgatgaa caaaccatct gataactttt ctttccgatt
     2581 tgcgtttcga gcaaagtcaa aaaaaacgag tccaataaaa taaaaaggta aaaattttca
     2641 agttttataa aaaacaaaac atatcagaac aggatcgaat cggtcctgtt ctttttttga
     2701 gaacacaaaa agaacaaata ccaaaaaaat tttcctgaag ttcactattt ttatgataca
     2761 atatccaaga taaggaggga acatgttgaa agaattaaaa gtaactgacc tcaaaaaaac
     2821 ttatggtgaa aaagatttgt ttgaccagat ttcttttttg gtccacgaaa aagaccgtat
     2881 cgggttgatt gggacgaacg ggacgggtaa aacaagtctc ttaaatatta ttgccggtat
     2941 cgatagcagc gacggtgatc ggcaaacagt gttttatcca actgattacc gtattggtta
     3001 tctttctcaa gcagctgaat tttccgatga attaactgtt ctccaagcag tctttcaagg
     3061 aaacagcccc ttgatccaaa ctgttcgagc atatgaggaa gcattgatcg aactagggga
     3121 aaacggagaa gaccctgatg tgcaaaaacg ttatgctaaa gcagaagaac aaatgaataa
     3181 agaagatgct tggactacag ataccaacgc aaagattatt ttgcaaaaat taggaatcag
     3241 tgaattagat aaaaaaatca gtactttatc aggtggtcag aaaaaacgag ttagtctggc
     3301 gcaagtgttg attgatgaac ccgatttact attactagac gaaccaacaa accatttaga
     3361 ttatgaagcg atcgagtggt tggaaaacta tttgaaacaa taccgtggcg cactattgat
     3421 ggttacccac gatcgttatt ttctagatag agtagcaaac cgaatcttcg aactttcatt
     3481 tggaaaatta tacgagtata aaggaaatta tgaagcttac gtcttagaaa aagcagaacg
     3541 tgatcgtgta gaagtagaac aagaagaaaa gcgcaagcgg ctttataaac aggaattagc
     3601 ttggatgagg gctggcgtac aggcacgtgg caccaagcag caagcacgga tcaaccgttt
     3661 cgaagatcta aaggagaact tgtaccaagt aaaccaagaa gatgatttag agctgaacct
     3721 agcgacacaa cgtttaggga aaaaagtact ggaaatcaaa gatggcagtt accgtataaa
     3781 tgaccaaaca ctgctagaac atttagattt attgattcaa tctcgggaaa gactaggtat
     3841 tactggtaaa aatggtgcgg gaaaatcgac attgcttaat attctagctg gcagaattcc
     3901 cctggatagc ggtacaatgt ctatcggtga aacggttcat cttgcgtatt atacacaaga
     3961 aaatgaagaa atggcaccag ataaacggat gattgcttac ttacaagaag ctgctgaaga
     4021 agcaaaaaca gcagatggca gtcaaatcgg agtagcagaa ttacttgaac gatttttatt
     4081 tccaagattt atgcatggga cactgatagg caaactttca ggcggagaaa aacgccggtt
     4141 atttttgttg aaactgctga ttcagcaacc taatgtttta ttattggatg aaccgaccaa
     4201 tgatttggac atagcaacat tgacgatttt ggaagattat tttagaagtt ttccaggtgc
     4261 tgtgatcact gtttcccatg accgttattt cttggacaag gtagcagata agttattagt
     4321 attccaagga aacggaaaac aagaacttta ttacggaaac atgtccagtt atttactgaa
     4381 gcaaaaagag acccagcagc ctgctgaaaa agcgaaacca aaaatacaga gcaaggaacc
     4441 tgctgaaaag aagaaattat cttacatgga acaaaaagaa tgggaaacga ttgaagatga
     4501 gattgcagaa cttgaagaaa aaatctcatt gctccaagaa gagatgaatc atcaaggaga
     4561 taatttcact cgattacaag aattgcaaaa cgacgtatct gaaacagaag cacaacttga
     4621 agaaaaaatg gcacggtggg aatacttgag tgaatgggta gaagattagg aggaaaaagg
     4681 atggaacaag cgtatcttga cttaggaaga aaattgttgg aagaagggca tcgtaaagaa
     4741 gacagaacag gcacaggcac attaagctta ttcggatacc aaatgcggtt tgatctgcaa
     4801 aaagggtttc ctttgttaac aacgaaacga gtaccttttg gattgatcaa aagcgaattg
     4861 ctttggtttt taaaaggaga tacgaatatc cgctatctat tacaaaataa aaaccatatt
     4921 tgggatgaat gggcatttga acgctacatc aaaagtccag actataatgg accagatatg
     4981 actgattttg gccatagatg tttgacagat cctgaattca atgaagaata taaaaagcag
     5041 agcaagcttt tttgcgagaa gatcctaacc gatgattcct ttgctgaaac atacggcgat
     5101 cttggacaca tctacggcta tcaatggcgt cactgggaaa caaaagacgg tggctttatc
     5161 gatcaaatca aacaagtgat cgaagcaatc aaaaagactc ctgattctag aagattgatc
     5221 gtttctgcat ggaatccgga agatgttcct agcatggctt tgcctccttg tcatacgatg
     5281 tttcaatttt atgttcaaga gggacggcta agctgccaat tgtatcaacg cagtggggat
     5341 gtgtttctag gcgttccgtt caacattgca agttatgcgt tgctgactca cctaattgca
     5401 catgaaacag gtttagcggt tggcgaattt gttcacacat taggagatgc tcatctttat
     5461 atcaatcata tcgaacaaat gaaagaacaa ttgagccgag aaatacgcgc atttccgact
     5521 ttagttttga atcaggaaaa gcagtcagta tttgattttg agatggaaga tatccaatta
     5581 gaaggatatg acccacatcc ggcgatcaaa gctcctatcg ctgtttagaa tcagaaagga
     5641 tgagaaaatg ttagctgcaa tttgggcaca ggatgaaaac ggattgattg gaaaagaaga
     5701 ccgtcttcct tggcgtttgc caaatgatct gaaattcttc aaacaaatga cagaagcaaa
     5761 tacactagtg atgggacgaa aaacattcga agggatgggt agccgtccgt tgccgaatcg
     5821 tcagacgatt gttttgacga gagattcttc ctataaagca gaaggcgtgc atgtcatgca
     5881 tagtgtggaa gacgtgcttg ctctggaaaa agaaacagac ggtatctttt tcatcgctgg
     5941 agggtcagct gtttatcaag aatttctgcc attttgtacg atattatacc gtacagtcat
     6001 ccatcatgta ttcgatggag atgcttattt tccacctgtt gactggtctg attggtcact
     6061 gatcaatact agtcaaggag aaatggatga aaaaaatcgt tatccgcatc aatttgaaac
     6121 gtatcagcgt aatgagaatc ctgtaaaaag cgggacaggc gaattgacga tataaaaaag
     6181 ctatggatag actagctgtt atttagagct gtcccatcca tagctttatt ttttttatta
     6241 agtaaagaat aaaacagaga agtacataaa acctgcacct aacataacaa acaaatgcca
     6301 aacaacatgc atatagcgga tagaattcaa gctataaaag aatgcgccta tcgtgtaaga
     6361 aagcccgcct gctaccatca aaataacacc agtcattcca agagagtgat aaagaggaac
     6421 agcagccacg acacagagcc accccataaa aatatagatg atcgtagata cttttgatac
     6481 tctctcccgt ttatgcaacg tcaacgcttt atatataatg ccaagaatcg caaacaacca
     6541 gattaataca aataaagtcc aacctagcca tcctcggata ctcagcaaac aaaaaggagt
     6601 ataacttcct gcaatcaaaa gaaaaatcga actatgatcg aatacttgga acaccttttt
     6661 tgcttttgta aaaatcaatg aatgaaacaa agtagacgtt aaaaataaaa gaatcatcat
     6721 cgatccatag atagcataag agacaatatg gagggaggaa tgcaagtggg caccttttac
     6781 taaaagaacc actagtcccg cgatactcaa tccagtccca attccatggg tgactgcatt
     6841 aaacacttca tttaatattt catacgttcg attaaatttc gttttttcca tgaaaacggc
     6901 tcctttacta ggtgtaatta atgaaaaatc attatttgtc tgttatacta aaaatagaaa
     6961 ttgtcttaca tgtagtttaa catgaataag gcatcttgtt aaagaaagag agagagaaaa
     7021 taatgacaaa tgtaaaaatc gtaacagatt cttcatgtac gatggaaaaa aatgtacgcg
     7081 atgctttagg cattcgtgtc atgccgctat ccatcatgat cgacggaaca gtatatcccg
     7141 atgatgacca actagacggc gaaagattta tggaaatgat ggcgaaagca aaaaatctgc
     7201 ccaaaacaag ccaaccacca attggagaat tcatctcgat gtatgatgag ctaggagaag
     7261 acggcagtga agtgatttcg atccatatga caaaaggctt gagtggaaca gtagaagcag
     7321 caagacaagc cagtaatcta acaaaaacga aagtaacggt tatcgacagt gataccactg
     7381 accaaggatt atcttttcag gtaattcgag cagctgaatt agcaaaagaa ggtaaaacag
     7441 tagagaaaat ccttgaagaa gtcaaaaaag tacaagacaa tacaaaatta tacatcggaa
     7501 tatccacact agataacttg gtaaaaggtg gaagaatcag ccgaacaact ggtttgctgt
     7561 ctagtgtttt gaacatacgc gtggtgatga atttcgccca ttctgaatta atccctgtaa
     7621 ctaaggggcg aggaaagaaa acgttcgata aatggtttaa taatttgaaa aatgaactga
     7681 aagaattgcc aaatgtgcga caaatcggta tttcacatgc ggatgcaaaa gaattggcgc
     7741 aagaattcaa agagggtttg caagaattat tcccggacat gcatatccct gtactgcata
     7801 cgaatccggt tatcgctacg catactggta aagacgcgtt tgcaatcatg tattatgtag
     7861 attagtcgaa aagaggataa gccctcagcc tgaaaggctt gcagcttatt ctctttttct
     7921 ttataaaatt aggaaaataa ttgttttctt ttttcataaa gactaccttt ttgataaaat
     7981 ttttgataac attagaacca atcgaataca agaggtgaaa aaatgaaatt aacgaaacga
     8041 catttgatcc tcgttttgtt cccattatta attgcgatca tcatctatgc aggtttagct
     8101 gcttctatac cgaaagctgg cccactaatg aaaccagtaa ctactgtgag agaagatcca
     8161 aataaaaaag aaaaacttca ctacatagct atcggagatt cgctgactga aggagtagga
     8221 gatcaaacca agcaaggagg attcgttccc cttgtcgcaa atgatctaaa agaccgttat
     8281 aatttgaccg cggttgaaac agaaaactat ggtgtaaatg gtgaacgaag tgaccagatc
     8341 ttaaagcgag taaaaacaaa agaagaaatt cgaaacaata tcaaaacagc tgattttatc
     8401 acactgactg ttggcggtaa tgatttgatg aaagttatcc aaaataattt attcgggtta
     8461 acggtcaatt cctttaaaaa gccactgaaa aactatcagg aaaatgtcac agaactattg
     8521 gaagaatttc gatccctcaa taaaaccgca cctatttaca ttttaggcat ctacaatcca
     8581 ttttatttga actttcctga gatcactgac atgcagacaa ttgttaacaa ttggaacaaa
     8641 ggaacagaaa cggtgacaaa aaaaatgccg aactgctact ttatcccaat caatgatttg
     8701 ctttatcaag gattgaatca ggaagtaggt gtcacagaag aaggaacaga tcaaacaact
     8761 agcacgacag aaaatctaaa tggcatcaaa aataatgtgc tctatgaaga agatcatttc
     8821 catccaaata atttaggtta tcaattgatg gcaaatgcag tgagagatca gctgatcgag
     8881 acacaaaacc aatggttaga gaaggaggat gatcaataaa catgaaaaca agatctgacg
     8941 tatcttcaaa caaaacaaag aaaacaaatt actggaaatt cgccttcctt atccttcttg
     9001 gggtagtact tggttcgacc gtatttttag gcagccgtat ttttgctaat cgggaaccag
     9061 aactcccaga aataccagct cttacggaaa gacaaggaga tcctgtctta acgatcaatt
     9121 caaataaaga aaaagtcaat caaatcatca gctttttcct tagtgagtat caaaaagata
     9181 gtgatattga atataaattt tatttggaaa atgaagcact actcaatggt actttcgaag
     9241 tactaggttt tcctatcgat ttctatttat attttgatcc gtatgtgatg gaaaacggaa
     9301 atgtccaact aaaagctaga agtctttcta tcggaacatt gagtttgcca atcaaagatg
     9361 tgatgaatat gatcaagaga aactataaat taccagattg gatcgaagtc aatacagatg
     9421 atctgactat catgcttagg ttggatcaat tccgaatgca aaacgggatg tatattaaag
     9481 cagataaaat cgacttagta aatgatgaca ttcggtttag tctatatttg ccaaaagata
     9541 cctctgatgc ttcaaacaac tcaaatacca acaaaactag caccagcagt actgaaagca
     9601 agacaaaaga aaataaaaaa tcatcggatg agtagtatta gagggggaat cataatggaa
     9661 cgagcaatat ttgcaggagg ctgcttctgg tgtatgatcc agcctttcga cacgcttcct
     9721 ggtatccata cagtgatgtc aggatatact ggagggcatg taccagatcc gacttatgaa
     9781 caagtaaaaa gccaaacgac tggacataca gaagcagtgg agattatcta tgatccaaca
     9841 ttgatttcat atgaacaatt agtagaactt tactggcagc aaactgatcc aactgacgct
     9901 ttcggacaat tcgaggatcg cggtgacaat tatcgcccgg tgatctttta tttgacagaa
     9961 gaacagcgga tcattgctga aaaaagtaag aaacagttag ctgaaagtgg acgtttcgaa
    10021 cagccaatcg ttacaaaaat cgaaccagcc caaccatttt atctagcaga agaagaccat
    10081 caagagtatt ataaaaagca tcctgaaaat tttaaaagaa accatgcacg ccgggctgcc
    10141 tttatcgaag aaaattggag gaacgaaggg tgaatcgaag cttttttcat tatttaatga
    10201 cactgcgagg cggtggcaca catgatgctt tatctgtttt cgctacagaa gccggtaaag
    10261 acatccaatt tcctaagcac agctctagct acgaagaaat ttctaattat ctggaactga
    10321 atgtcgacta tttgccgtcc atggatatct ttgacgaagc atgggaaaaa tatttagaaa
    10381 ataatcatga catataagag atagttttgt actttatgat caaaaaagtt ttttgtccaa
    10441 tagcctaatt gaaagaatta tttttatttc agctatctag tcgcattttt ttgatatttt
    10501 tcttataata aagaaagaat gaaaaaaaga gggatggatt gatagagatt gacaaaaaaa
    10561 caacgctata ttaatcgctt ctggcttgta ttcaaatact tacttgtttc tgtattcctt
    10621 gcgttcatgt tgcggggatt tttattcatc ccggtaccag tggaaggcaa ttcaatggaa
    10681 aacgtgttga agcaaggtga tatggttgtg atggaaaaat tttcggagat cagacgattt
    10741 gatattgttg tttttcaatt agcagatggc acgatttata tcaaacgagt aatcggtctt
    10801 ccaggagaaa atgtcagcta ccaaaatgat caattgaaga tcaatgggaa agtagttaag
    10861 gaaccttatc taactaaaaa cctgaaatca gatcatgcaa atgcatccta tacgacggac
    10921 tttacactac aagagctaac gggacaatcc aagcttccgg aagataatta ttttgtactt
    10981 ggagataatc gaagagtttc aaaagatagt cgttcgtttg gcacgatcaa taaaactgat
    11041 attctaggta aagcaagatt tgtctattat ccacttgatg aaataaaatg gatccagtga
    11101 gaaacaataa agcgtgcagt ctgttgagac tgcacgcttt attgtttaat ccaaaatcag
    11161 tttactacct tagtacgtaa aatcaaacag ctcgtttaaa cacttttcta gcctctaaag
    11221 ttttgataaa tgaataaaca accattattt gaatagggat ttggatcata ttcctgatga
    11281 atctacctag aaaaattact ttgaaatctg caccaaacaa aacagacatc agccaagaat
    11341 tgataaaaag gctgactggg atcataatca ataattgggc agcgatgacg gaaggcaggt
    11401 ttatcttttt atgatataaa aataagccat taataaatcc accaaggaaa gccgtgataa
    11461 gaaaagcagc ttgaaacgtt cctgaaccaa atatcgtgaa atttaaagtg ttcgacaaca
    11521 tttcaagcaa accagcccag atcggtccat atagataacc cataagcgcc atacttaaaa
    11581 atccgaatgt gatttgaaaa taagcaggac caatcgctat cttgtataag atgacatcaa
    11641 gggctaataa aagagcagag gtcgacaaca atcttgcatt catttcgggc aatctaaaaa
    11701 cgtcttttgc aatcaaagtg ttcaaaatat tctctccttt gtcagttaat aataaaagga
    11761 taccataaaa tcaccaatgt gtcatgacac attggtgatt tacatacact ggcatctaac
    11821 aattaaaaaa agggtgagaa gactgctttt ataaactgta aaaaagagat tgtacgaata
    11881 aaaaaaatag aggcggagaa ta
//
LOCUS       DACEXQ010000068        11898 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.068, whole genome shotgun sequence.
ACCESSION   DACEXQ010000068 DACEXQ010000000
VERSION     DACEXQ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11898
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.068"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            265..1137
                     /locus_tag="IXH04_001308"
     CDS             265..1137
                     /locus_tag="IXH04_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1851928.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            1134..3092
                     /locus_tag="IXH04_001309"
     CDS             1134..3092
                     /locus_tag="IXH04_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1851929.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(3343..4194)
                     /locus_tag="IXH04_001310"
     CDS             complement(3343..4194)
                     /locus_tag="IXH04_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1851930.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            4317..4784
                     /locus_tag="IXH04_001311"
     CDS             4317..4784
                     /locus_tag="IXH04_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAQ1851931.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(5047..5529)
                     /locus_tag="IXH04_001312"
     CDS             complement(5047..5529)
                     /locus_tag="IXH04_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1851932.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(5629..6492)
                     /locus_tag="IXH04_001313"
     CDS             complement(5629..6492)
                     /locus_tag="IXH04_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1851933.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            7090..7938
                     /locus_tag="IXH04_001314"
     CDS             7090..7938
                     /locus_tag="IXH04_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851934.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(8109..8450)
                     /locus_tag="IXH04_001315"
     CDS             complement(8109..8450)
                     /locus_tag="IXH04_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HAQ1851935.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SMEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            8536..9243
                     /locus_tag="IXH04_001316"
     CDS             8536..9243
                     /locus_tag="IXH04_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAQ1851936.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            9230..9553
                     /locus_tag="IXH04_001317"
     CDS             9230..9553
                     /locus_tag="IXH04_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ1851937.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(9642..10679)
                     /locus_tag="IXH04_001318"
     CDS             complement(9642..10679)
                     /locus_tag="IXH04_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1851938.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(11126..>11898)
                     /locus_tag="IXH04_001319"
     CDS             complement(11126..>11898)
                     /locus_tag="IXH04_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAQ1851939.1"
                     /translation="MVAHIWGDKPEFFREMSIAMAEMGFQGIDINMGCPVPNVAERGK
                     GSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISHLLHQDIANLSVHL
                     RTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGEELAEKYGVDGIMI
                     GRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSITGLHRFFKIYIKG
                     FPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
BASE COUNT         3503 a         2464 c         2148 g         3783 t
ORIGIN      
        1 agtttcttaa tttatttctt ccttttattt ctgataattc cagtaaatta ctataattat
       61 aaaaaaagaa ttgacaaaac ataactgtct atataaaatt gattacgtta acaataattc
      121 gtttcatgtg actagatcat actaggcatg aaaaatctct gaggaacatg gtttccctag
      181 gaggtttttc gtgtctattt ttgtttggcc gcaatcaact ggtctagcaa acgtgaaaac
      241 caaatcttca aaaagggtgc gcgcatgaaa atcaaaaaga tctttaatca aaatgcagta
      301 ttggtagatg acggtggaca ggaaaaagta gctattggaa aagggattgg atttgacaga
      361 aaaagaaatg atttgatttt tgatcgtgat gtcgaacgga tatttgtcat ggaaccagaa
      421 ggtcaaatca agttgcaaaa tttacttaat cagattgatg aacgcttttt atttgcagca
      481 gaaacgatca ttgatcatgc ggaaactgtt ttgatggaaa agttgaatga acatgtattg
      541 atcgctttga ccgaccacat tgctttttct gcggagaaca ttaataacgg tatcgtcatc
      601 cgcaacaaac ttttaagaga aatcgaagtg ctttacagtg atgaattcag tattgctcaa
      661 tgggcagtag agtatctgac aaaagagctt gggattccat atacttatga cgaagcagga
      721 tatatcgcaa tccatattca cagcggacga agcggtcgga acaataacca tcgcagtatc
      781 cgtgaagtga cgattgtatc tgacatcatc catttagtgg aatcagaatt ggaaacagat
      841 atccattcag aaccattttc actgaattat tcaagactgg tgaatcattt acgactgctt
      901 ctacaacgaa cccatgcgca acaatatgct gttttagaca cggaaatcgt agaaatggtc
      961 aaacgaaaat acccagaaag ctataagatt tctaaaaaaa tccgtgtgtt gctgaccaaa
     1021 gaataccagc tcgcgatcac gaaagaagaa ctgggttact tggccatcca tatcgagcgt
     1081 ttaagaagcg caatcgtaca aacaaatgtt aacaatcatg aggaggaaaa aaattgaaag
     1141 catatctaca acgaatggga cgttcccttc aacttccagt tgcggtctta cctgctgctg
     1201 ctttactagt agggatcgga aactggtggg catcttatag taacgacatt gtcgctcatt
     1261 ttttacaagc tggcggtaac gcagttttag gtcaattacc tttgctgttt gccgttgggt
     1321 tagcactagg gatgtcagtg gacaaatcag gtgctgctgc cttggcaggt ttggtcgcat
     1381 ttgaagttcc agtaaatgtt ttaaaaactg attcagtagc tactttattg aatatcaaac
     1441 cagaatcagt tgatccatct tttgctcaga tcagtaacgt atttattggt attattgccg
     1501 gattgattgc ggcagctctt tataatcgtt tccacgaagc aaaattaccg atggctttat
     1561 ctttctttag cggcaagcgt ctagtgccaa tcttggctgc ctttgtcatg ttgctgatca
     1621 gtgcagtgct actgctcgta tggcctccgg tttactctgc tttagtttct tttgctaaat
     1681 tcattttgag tttaggctgg ggtggtgcag gtctttatgg cttcttcaac cgtttactga
     1741 tccctacagg attgcatcaa gcaatgaact ccgtgttctg gtttgacgta gcaggaatca
     1801 atgatattgg taaatttttg gcttctgaag gaacaaaagg cgtaacagga atgtatcaag
     1861 ctggattttt cccaatcatg atgttcgggc ttccagcagg agcatatgct atttacagaa
     1921 atgcacgtcc agaaaacaaa gcaaaaacag cttccttgat gctagcagca gcttttgctt
     1981 cattctttac tggtgtgaca gaaccacttg aattttcatt tatgttcgtt gcttggccat
     2041 tatatgtctt gcatgcaatt ttcactggga tttctttagc agtgagtgca ttcttccatt
     2101 ggacagcagg atttgccttt agtgccggat ttgtcgatta cttcctatct ttgaaaaatc
     2161 cgattgcgaa tcagccattg atgttgatcg tccaaggatt agtaacagca gtgatttact
     2221 actttggctt taactttgct atcaaaaaat tccatctgat gactcctggc cgcgaggaag
     2281 cagatctttc agatgacgat actgcaacga ctaattcaga caataaatat gcggcacaag
     2341 caagcaaaat ctacgctgca ttaggcggag ccgacaatgt aacttcgatc gataactgta
     2401 ctacacgcct tcgtttgcaa gtcaaagata caggcctgat cgatcaaaat aaaatcaaag
     2461 ccactggcgt acctggtatg aagatcatcg acggaaaaaa tgcgcaagtc atcgtaggta
     2521 cagaagtaca attcgttgca gatgagatgg caaaactaca tggtggtgct gcagcacgac
     2581 ctgcacaaac aaacaccgtg gttaaaacag aaacatttgc cggtgaaacg atcgaacaag
     2641 atatttacgc tatcgcaaat gggaaactga ttccaatcac tgaagtttca gatgatgtat
     2701 tcgcggaaaa aatgatgggt gacggctatg ctgtattacc agaaaatgga gaaatcttct
     2761 ctccaatcgc tggtacgatc acaaacattt tcccaacgaa acatgctgtc ggaatccaaa
     2821 cagatgctgg tatcgaagtc ttgcttcata tgggcatcaa cactgttgac ttaaaaggcg
     2881 aaccattcac tttatatgta gaagaaggtc aaaaagtcgc aagaggtcaa ttgattgctt
     2941 tagttgatct tgccgctatt caatctgcag gcaaaaacac ggatatggtc gttgtattca
     3001 caaatggcga caaagtccaa agcttagaaa tcgaacccgc acgtgatgta aaagcaaatg
     3061 ataaaatcgg ctctgtttcc aacaaagcct gagccatcgt cttccccaga cttttttatc
     3121 gataaacaaa caggagctga gacaaaaatc ttgtctcagc tcctttttcc gaataaaaac
     3181 ggcgttctcc tgccaactcc gcggtaataa gtccagctaa acgaaacata tgaagagcta
     3241 ttttttattt gactcttctt attacttgat gcagaacagc tttttcacaa aatttttctt
     3301 catactcaaa actaatcggc tttttcacaa cctctttata cattattcct gatccaatat
     3361 ttcttttaat gctgcttcaa gagaagttaa tggttttccc aatacttttt ctaaatcatc
     3421 agaggaaata gctaactgat cctttttaat atcatgttgg atcgctaaaa acatgtcagc
     3481 tgcttcttga gggaatccgg cttcttttag attttcgaca aacgctgcat cacttgcttc
     3541 aataatttct aactctttac ctgtcgcacg ctctaatgcc ttggctaatt cttcatagtc
     3601 agttagattg ccggataatt ctaagatttc tggagagtca ctttctacta gtacttttgc
     3661 tgcagcttct gcatattcac gtttcaatgc ccaacctgtt tttccatttc ctgcagcata
     3721 aacgaattga ccagttttta aagcctgccc aatgataggt aattcattct ctacatacca
     3781 attattccgt aaaaatgtat gggaaatacc tgaatcctta atcatttttt cagtgaattg
     3841 gtgatctggc gctaatacac tgatcgagtg atcggcatct gcaaaacttg tataagcgat
     3901 ataagatacc ccagctgctt gtgctgcctt cactacattc ttatgttcgg cttgacgatt
     3961 tcctggaacg ccagagacaa aaagcaatcg atctattcct ttcaacgctt ctttcattga
     4021 gtctaaatca tcataatcac caatacgtat attaaatcca gcttctttca gtgcttctcc
     4081 tttttcttca ctacgtacta gtacatagat ttcattttgt ggaaccatct tttttaagaa
     4141 ttccagcgca taaccaccaa aacctccagt tgctcctgtt actaaataat gcatatttgc
     4201 cactccctat tctttttatt tgttaagatt tacagctgta catttataaa ttacaccttt
     4261 atcataaaga gtgtggtaag ataagtcaag caaaaaaaat gaaaggatga caactgatga
     4321 agtattctct gcaatttagt gatgcaatcc atgtacttgc ctatattgaa atgtttcaag
     4381 atacccgatc actttctagt gagatgattg ccagtagtat cgaaacaaat gcagcaaatg
     4441 tacgtaaaat catgagtcag ctcaaaaaat ccggtttgat ctttacaaaa accggaaaac
     4501 cgcagccttc ccttgccaaa agtccagatc agatttcttt actagatgtc tatcgcagca
     4561 tcgaaggtaa tacacagctt atccaagtcg atccaaaaac aaatccaaat tgcgtcgttg
     4621 gtgcgaatat ccaagaagct ttagaagaaa gctacgctcg gctgcagaaa aaggcagaag
     4681 aagaaatggc taagatcaca cttgatagtc tgatacataa aatagccgtt ttggaaaaag
     4741 aaaaaagacc agaaaacaca ggcctcgtag agagattttt ataattctct ctactgtgcc
     4801 tgcatttttc tgatcggaat cgtctacata aattctttta ttctccgggg gacaaaagcc
     4861 ttgtcatgcc cctttttcct ctctaatgct caggtcaaag cgctgaagtc atgacgtctt
     4921 gataaacggt gttcaagtag ctgaccctcg gcattagttc tgaaaaatgc aaaatatgga
     4981 aagctatttc ccatttttcc tctctaatgc tcaggtcaaa gcgctacttt cacgacctct
     5041 atattatcat ggtttattga ctaccggtat ccagatttct cccatatatt tcccgttggt
     5101 aactcccatt tcgactacag catttggacc cccaacatag gcgtaatctt ctacttctct
     5161 taaaacatgc ccaaaggccg tattcgtcaa atcatttctt aattcttcag gcgattctgc
     5221 taaagcgctt acaacaagat acgttccttt tgggaatggg atgattcgcg tagtgtttgg
     5281 aacatcctga ctcgtttgaa caccgacata gtacatcatt ttcccgttat agggttcgtt
     5341 gactgtaaag aaataatgat tttcagcagc ttgtttcaat gcatctattg ttccattacc
     5401 gataactttt tgctcaaatg cttgtttctc tgcagccaaa ccgacaaaat ccgtattgct
     5461 actcttcaat tccacaccaa tacccgtcac cgtaaaatct ggtttttctt cgatcctata
     5521 ctttgccatt tctcccaccc tttctattat attaagttca tcttacataa aattaaaggg
     5581 aaatgacacg attacgctct tttttctagg gatattttta ttttttaact atttttttgc
     5641 tttcactgca atccaaatct cactgtaaac attactgaaa tcctctcccc attttgtaaa
     5701 agagatctcg ggagcttcga ctaacccata atctgaagaa ggaagccatt ctgcatagat
     5761 tttcccccaa gtatcttgta aagtttgggg aaatggccct ttattaggaa aaatggccca
     5821 agtgtgttct tctatagcaa gcatttctaa atcagcgtac ggattttctt ttgtcgtagc
     5881 aaagccaatc atgtgcatca aactgccctt ttcttccaag cggtctccat caaatccata
     5941 agaaacgttg attacttgat ttggatataa atctcctaac ttatgcatct cttccctttg
     6001 agtctccgtg atcgtctgcg ctaacttttg tatttcttga ttgactcctt caaatttgac
     6061 aggaactttt ttcgccacac ctgccacata aaactgttct ttcttttcga tacgaaattc
     6121 catcgatttt cctcctctga catcaataaa aaatgtcaag cgtggaaaag atttttgtat
     6181 gccgtatcta ctgatttctg aaggcagata tcctgaccaa tcacgaaaag ctcttgaaaa
     6241 accttcgact gtttcatatc catatctaaa cgccgtttct gtcactcttg atccatctag
     6301 aagatcgcga ttagccaaag ccaatctacg gtttttgata tagtccatta gcgacatccc
     6361 tgcaataaac gtaaagatgc gttttaattg atattcagag attcctgtat tttcggctat
     6421 tattttatag gaaagtcctt ctgtcaaatg ttcttcgatg taatcgatca attggttcag
     6481 atctttaagc atatttatct tcctccttga ttgccttttt atcatattat caaagaaagt
     6541 caattacttg atgaatcagg aaccaattta gtcagatgta gaaaatgttg tatggatcta
     6601 aagaaaattc tcaagcagcg tttcgtaacg gataattggt attatctgcc tatctatgtt
     6661 ttacctttag aaaaataaat tttggggaag acagattcta agtaaacaaa gcggtaaaaa
     6721 tatcttttat tcccttagtt aatccgctta ctacctactg tacaaagcaa attgttgttc
     6781 aaactgctga tcagaacgct gcttggattt ttctttgcga tacatcatac ctcttttatc
     6841 aattggaaca attatttatt tctaacaaaa tcacttcatt tttgtcagat aaataaaaaa
     6901 caaaaaaagt tccatcttta aaacacgcta ttcttttact ttttctcaca ttctctaaga
     6961 aaagaaaagg agaatgaatt tttgacaatt cgagcaaaag tgattatcaa atatgtaaaa
     7021 tcagctactt ttttcttaaa cgtttcttta tactaaatat aaataagtgt ttcatgaaag
     7081 gagaactaga tgaataattg gcttgagtat tttcctgaaa acgttttgga acgcggttac
     7141 agctatcatc tgcacggttt tgtccgtcat ttgaactata caagcaagta cttgtccgca
     7201 acagtttctg gcacggaaga ttataaggta gtgatcactt gggatgaaaa gaccaacatg
     7261 acttgtgatt gtctatatgc catagaagga aaaaaatgca aacacatggc tgctgtctta
     7321 ttcgcttacg aagaacgtcc aataaaaaaa tccaactact cactttccga actctcgtcc
     7381 cttgtttcct ccgctagttc ctctcttgta cgggagcttt taacagagat tcttatagaa
     7441 catccgcact ttatcgaacg cttcaaagtt aagatgccat ttcatgcaat aaattattca
     7501 gataaattaa caactatcat ccataaatac gatcatatta tcaagaaaaa caaaaacagg
     7561 aaaacagcga agttcatcat ggagatgcga aagttcatcc aagaagcagt tgaatcgctt
     7621 attcagcaaa atgcctatct tcccgcattc gaattgatca atgaagtgat tgctactctc
     7681 gaaacattct attgggagcc tgaggatgaa cgaacccttc ttctgataga ggactgttat
     7741 tatttatgga aagaattatt agcagaagca cctcatgcag aaaaaaggca aatgttctct
     7801 tggttcgtct gccaagtcga tcatactgac gcctcttatt caaaacgcta ctctataaaa
     7861 atcctgaaag aagactttcg ggaaaaagaa ttttctaacc aaaagaaaaa aatagataaa
     7921 aaactaaaga aacggtaaag aaagacgatt tcaatgaaaa atagcaagta aacgataaca
     7981 accatcctaa atccacaaga aatacagcag accttgcttt aaaaacacga atcatgcaaa
     8041 ggatgaagta aagaagactt aacgcgtacc caatattagt catttgacta ttaggtacgc
     8101 gttttgtttt ataagtcatg acaaatttcc ttcgttgctg gatagatttt ccggcattgt
     8161 tgatagattt tatgatactt ttctacattt tgtaagagag gttccgctgc atctgtatat
     8221 tggataaaat tctgacaaca actttcaggt gtgtcgaacc accctaaccc tagtgcagca
     8281 atcattgttg cgcctaatgc aggtccttcc tccatactta aacaatagac cgttgcatcg
     8341 aaaatatcag cttggatttg tagccatgca agatttttcg cgcctcctcc caccgaaaca
     8401 atacgtgtaa atctgcgacc cgctagttct ttacgtttca atcccaccat tgacaaatgc
     8461 tcattaaaaa tttagaatat tcacaagttt aacgacaatt atgaaaattt atgtcgtgct
     8521 aaaggagttt tgttattgga tgaaaagtta gatataaaaa ctgcgatcaa agacacgtta
     8581 cctaccgttt tcggttatat cggtattgga cttgcatttg gtatcgttgg gaaagctgcc
     8641 ggatttcatc cactagtcgt cacgttgatg tccctgctgg tctatgctgg ttctgcccaa
     8701 tttatcacag tcagcatgct tgctagtcac agcccattgc tttccatcgt tttctcgacc
     8761 tttctagtca attcccgaat gattctgatg agtatgacga ttgctcctta tttcaagaaa
     8821 aatcggctgc ttcaaaatct gctgattggc acattgctga cagatgaaag tttcgcactg
     8881 ggaatgaata aattgaacta tactggacaa aagttaaact ttcgttggat gaatacagct
     8941 aattggattt cttatttgac gtgggtgggt tcttctcttg ttggcgcact tttagggaat
     9001 tttattactg atccgaaaaa gtttggtttg gatttcgcca tcgtcgctat gtttatcggg
     9061 ttactttatc ttcaagtcat ttctgataga aacacctcta aacgattaca gttgatcctg
     9121 attggtctca ctttgattct ggtatatgtc ggactgattt ttatcccaag caacttagtg
     9181 attgtagttg taacactgat tgggtgcgga ttgggggtgt ggatcaaaca tgccttcttt
     9241 tgagtttaca ttgctgacta ttttaggttg tactttagca acttggcttt ctagggtcct
     9301 accatttgtc ttactgaaaa aattcgattt accgcaaccg ttgcttgagt atttaagttt
     9361 tgtcccgatc gtcattatgt ctgctttatg gttcagcagt ctatttaccc aaaatatcgg
     9421 acatctgccg cagatcaatc tcgaaaatgc tttggcttca gttcctacac ttttagctgc
     9481 tattctttca aaaagcttat tggtgatcgt cttggcagga attctttccc tttcgttgat
     9541 tcgcttatta taaataaaaa aacaagagct tatggcaaat tactgtcatg cgctcttgtt
     9601 tttatcaaag tcaaataaga aaaaaatcgg cgtattcttg cttatccgtt gaacggacca
     9661 ctaacaacga cttttcctaa catatgtcct gtttctacgt ctttggttgc ttgtttcaag
     9721 ttcttcgcat tcatcccatc tgaatagacc ttgctcaatg tactgcggat aatcccttca
     9781 tttgctaaag cagcaatgtg cgctaaagct tctccttgcg tttcgatttc atagttatag
     9841 tctgttttag cgaacatata ctcccagtcg aagctgatag attggttttt ccaaacacct
     9901 atatctaacg gctctttgat ccctacgatc gttcccacat gtccaaatgg tcggacaagg
     9961 tgcttgattt gatcaaagta gcgggtaata tcgaataata cagcgacata ctctacttga
    10021 tcgattccta attcacgaaa acttttactt aagtcgtgat gataatcgat tggatagtct
    10081 acacctgttt ttttcagcca ttcaaaattc ttcggacttg cagtcgcgta gacttctagg
    10141 ccagcccaat gcgctagttg attcaaaatc gaaccgacgc ccccggatcc gttgatcact
    10201 aagatttttt tcccgcgatt ggcattttct tctggagtta gaccaaattt ttcaaataac
    10261 aattcataag cggtcaatga ggtcaacggc aaggctgcag cttcttcatc tgaaagattt
    10321 tttggtgcaa gagcgacaat acgttcgtca actagttggt attcttgatt gcttcctgct
    10381 cttgttgttg tacctgcgta aaaaacgcga tcgccgacag aaaatttttg cacttcgtca
    10441 cctacagcaa caactttccc gactccatca aaacctaaga tacgtaaagc atttcttatc
    10501 ccgttatctt ggcgaagttt cgtatcgaca ggattaacag atactgcgtt gacttttacc
    10561 agaagatccc gtgaactggg tacaggtatc tgttcttttt catcaataaa gctttttgga
    10621 tcatcgatcg gcaacccttc ataaaaaccg actaccttca ttaattcctt ttctgacata
    10681 cgaattcctc cttagcttca tcaagttgga gcatccatac agtgttcatt tcaatatata
    10741 tagtatgcta tttgttccac cttttttatt catcataatt attctcttac ttaaagtaaa
    10801 ctatttgcac ttttttggtt aaaaaagagg ttgcgacaag acttttgtca caacctctta
    10861 taaacggtgt tctcctacca actcttcggt cacaagtcaa aatattcatc aaacatgaga
    10921 agctgttttt tgaaattgct ccttgtgact cagagttaaa cggcaggctc acaacctctg
    10981 atataaacgg tgttcaatca actgaccctc ggtattagct caaaaaacga caaaatatga
    11041 agagctattt ttgtttggct gttcttatta cttgatacag gacagctttt tcacgacctc
    11101 taattcacgg tgttcaagca agaaatcatt gttcactgaa aagcgttgga tgttcttttt
    11161 caaaggtctc taagatctct cgtacttcat cagtagattt cgtattcatc aactgaactc
    11221 gcaagtcatt tgctcctgga aatcctttga tatagatttt aaagaaacga tgtagtcctg
    11281 tgatggaacg cgggatgatt tctgaataat gatcttgcag atctagttgc agacgaagta
    11341 gtccgatcag ttctttttct gtatgttccc tcgggtcttt ttcaaatgca taaggattct
    11401 tgaaaatccc tcggccaatc atgatgccat caacgccata tttctcggcc aattcttctc
    11461 ctgtctggcg atcggggata tctccattga tcgtgattag tgtttgtgga gcgatttggt
    11521 cacgtaaagc aatgatttta ggaatgactt cccaatgtgc atctacttta ctcatctctt
    11581 tacgcgtacg caaatggaca gaaagattgg ctatatcttg atgaagtagg tgactgatcc
    11641 attcttccat ctcagacatt tccgtatagc cgatacgcgt cttgacgctg acaggcaaac
    11701 cgccagcttt agctgcttca atcaattgtg ccgctacttc tggacgaagg atcaagccac
    11761 tgccttttcc acgttctgct acattaggga ctgggcagcc catgttgata tcgattcctt
    11821 ggaagcccat ttctgccata gcaatgctca tttctctaaa aaattctggc ttgtctcccc
    11881 aaatatgggc aaccatcg
//
LOCUS       DACEXQ010000069        11895 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.069, whole genome shotgun sequence.
ACCESSION   DACEXQ010000069 DACEXQ010000000
VERSION     DACEXQ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.069"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..707)
                     /gene="hflX"
                     /locus_tag="IXH04_001320"
     CDS             complement(<1..707)
                     /gene="hflX"
                     /locus_tag="IXH04_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAQ1851940.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWR"
     gene            complement(709..1626)
                     /gene="miaA"
                     /locus_tag="IXH04_001321"
     CDS             complement(709..1626)
                     /gene="miaA"
                     /locus_tag="IXH04_001321"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAQ1851941.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(1630..2367)
                     /locus_tag="IXH04_001322"
     CDS             complement(1630..2367)
                     /locus_tag="IXH04_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ1851942.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2458..2985)
                     /locus_tag="IXH04_001323"
     CDS             complement(2458..2985)
                     /locus_tag="IXH04_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAQ1851943.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3165..3758
                     /gene="clpP"
                     /locus_tag="IXH04_001324"
     CDS             3165..3758
                     /gene="clpP"
                     /locus_tag="IXH04_001324"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAQ1851944.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(3862..4347)
                     /locus_tag="IXH04_001325"
     CDS             complement(3862..4347)
                     /locus_tag="IXH04_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAQ1851945.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(4500..4697)
                     /locus_tag="IXH04_001326"
     CDS             complement(4500..4697)
                     /locus_tag="IXH04_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851946.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(4792..6552)
                     /gene="efrB"
                     /locus_tag="IXH04_001327"
     CDS             complement(4792..6552)
                     /gene="efrB"
                     /locus_tag="IXH04_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAQ1851947.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(6549..8279)
                     /gene="efrA"
                     /locus_tag="IXH04_001328"
     CDS             complement(6549..8279)
                     /gene="efrA"
                     /locus_tag="IXH04_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAQ1851948.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            8672..8884
                     /locus_tag="IXH04_001329"
     CDS             8672..8884
                     /locus_tag="IXH04_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAQ1851949.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            8886..10565
                     /locus_tag="IXH04_001330"
     CDS             8886..10565
                     /locus_tag="IXH04_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ1851950.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(10819..11019)
                     /locus_tag="IXH04_001331"
     CDS             complement(10819..11019)
                     /locus_tag="IXH04_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ1851951.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(11169..11330)
                     /locus_tag="IXH04_001332"
                     /pseudo
     CDS             complement(11169..11330)
                     /locus_tag="IXH04_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            complement(11710..11783)
                     /locus_tag="IXH04_001333"
     tRNA            complement(11710..11783)
                     /locus_tag="IXH04_001333"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11747..11749),aa:Ile,seq:cat)
BASE COUNT         3487 a         2564 c         2068 g         3776 t
ORIGIN      
        1 cgccatctct tagtcaatgg atctaacgtt gcaaacaatt gatctttcga ataagtatcc
       61 gcttgtgtca atagattcaa gatagttgac ttcccggcat tcgtgtagcc aataagtcca
      121 atctgaaaaa tctcgctatt ttttcttttt tggcgattgc gctctcgatg agcttctact
      181 tctttcaatt ctttcttaac agcaaaaatt ttattccgta tatgtcggcg gtctgtttct
      241 aactttgttt cacctggccc tcttgttccg atcccaccgc caagacgaga taatgttttc
      301 ccttgcccta ctaaacgagg cagcaggtat tctaactgag caagttcaac ctgcagcttc
      361 ccttctttcg aacgagcacg catcgcaaaa atatctaaga taagctgcac gcgatcaatt
      421 accggaagac caacagcatc ggtaatcagt tggctctgtc tcggagttag ctcatgatta
      481 aagatgatca aatccgcttc atgcgcatcc gcttgttgaa ctaattcttg taatttccct
      541 ttaccgataa tcgtttgccg atccacttgt ggtctctttt gtgtaattgt gaatacgact
      601 tctcctgatg cggtattcgt cagctgaact agctcttcca ttgactctga aaagtaacgc
      661 tggtttgctt cggtttctac ccctacgatg atcacttttt cttccatttt attccgcctc
      721 cagccatttt ttgattgttt gctcaatctg acttttttga tttggctcac ttataagatc
      781 aaaccactgt gcatccaaac ggttacgaaa ccacgtcaac tgacgtttcg cgtatcttct
      841 cgaatgtagt ttgacttgtt ctgttgcttc ttccaatgtt tctttccctt caaaataagg
      901 gaaaaattct ttatatccaa taccttgact ggcctggact tctccaagct catacacctt
      961 acgcgcttct gctactaaac cttctgctag catcaaatcg actctttgat tgattcgttc
     1021 ataaagaata ctacgcttcg tatctaatcc aatcatgagg taatcataca attgctttgg
     1081 ctcttcttta ggtgccgtga tactatgtcc tgtcgtctct aaaacttcca gagctcttat
     1141 cactttgcgt tgattgttcc agtggatttt ctcagcagct gccgggtctt tttctttcaa
     1201 ctgattccat aatgcttggg aaccaattgt ttcagctaac gtttcatact tttttctaat
     1261 ctcaggattc gcttcttctg tcgctcctaa ctgataatca tatagaaggg attggatata
     1321 gagacccgta ccaccagcta tgataggtag gtgccctcgg ttcgcaatct cggtaatcaa
     1381 ttgtcgacct gaatgctgaa aatcagcggc tgagtaacgt tgagtaggtt cgatcacatc
     1441 aatcaaatga tgcggaacat tattcatctc ttcttctttg atttttgctg taccgatatc
     1501 cagattttta tagatttgca atgaatcacc actgatgatc tctcctccaa atagttgtgc
     1561 gagttccaca cttagcgctg ttttaccgac tgctgtcgga ccgacgatca ctaatacttt
     1621 ttccatattt tacctacttt tcgttgattg tttccaccga agagctttct ccggaaaatc
     1681 agttattatt ccatctattt tgagttcttg acatttggcc atttcttctt catcgttaat
     1741 cgtccatgca cgaattgctt ttggaaaatc agctgcctgg actggatgtt cgaacaacca
     1801 agtatttttt ggatgtactc cttctatata aggggtttct gctgctagtt ggatcttttt
     1861 atcagaagta ctcatcaata agtccagctg tatctccggt tccagtaatg cgattttttc
     1921 caaagaatcc aaatgaaagc tgcaatatgc atgttgaaaa ggccaatctc tactttttaa
     1981 tgtttcggct aatttttctt cgataccggg atattcatac tgatcagttt taatttcaat
     2041 cattagcatc cctcggtaat ttttttggag gagcagtcct aacacttctc tcaattcagg
     2101 aactttagct gctggaaatt ctttgctgaa tttgccccct gcattcaatt ttttgagttc
     2161 ttccaatgtt ttttctcgaa tcagcccttt accgttagtc gtccggtcca cttcttcgtc
     2221 gtgcataacg atcaaataac catctttgct tagatggaca tctagttcga taccatctgc
     2281 acctactcgt acagcttctg caaatgcagg aagcgtattt tctgggtggg tgccgctgct
     2341 tccgcgatgg gcatagattt gtaacatctt tcttcactcc gttcagttct attgtatcat
     2401 taagaaaaaa agaaagcatt accacgctct catgtgcaac ggcaatgctt caaaatatca
     2461 gctccaaaca tcatctttcg agaagaaacc aatcggctgg cgcgtcatag tcttcaactg
     2521 agcttttttt tcattgatca tttcagtcag actatccgta aaattctcta aaatcacgcg
     2581 tccgccttta taagcatatc caccaatctt cattgtttca tctggagata aaatcacttt
     2641 tcgatgggag atattcttaa atagctcgtc taaaatataa tttggcagat aaaaactatt
     2701 ttgcgctgaa aatgattgga gatacgcaaa atcgtcaatc gtgatataac aatcatcaaa
     2761 tgcatcaaag ctgacatatt cttccatcag cgttgtccgt ttttgcaaga tctcgttgat
     2821 ctttttattt aattgattca gcgtctcgat ttctttgact ttcaattcca attcacgtct
     2881 acgctgttct ttattttcca agtgacgcat ttgcagtatc atgccgatat agacaactac
     2941 agcacctgtc agcactccga taaggccagt aataccaact atcaatctgt tgttcctcct
     3001 tgttttgttt ctctgcttat cataccataa agcgggttta tgcgactaat ttgcctaagt
     3061 tttttatttg accttttttg accaaaaatg tatactacat tttaggtatt agaaactaaa
     3121 ttatggtaca ctatttctaa acgaaatcaa ggaggaactc tattatgaat ttaattccta
     3181 cagtcattga acaatcttca cgaggtgaaa gagcttacga tatctactca cgtttattga
     3241 aagaccgtat catcatgtta agcggccaag taacagatga tcttgctaat tcgatcatcg
     3301 cccaattgct tttcttagac gctcaagatt ctgaaaaaga tatctatctt tatatcaatt
     3361 ctcctggtgg ttcagttaca gcaggtatgg ctatttacga cacaatgaac tttgtcaaag
     3421 cagatgtcca aacaattgtt atgggtatgg ctgcttcaat gggaagcttc ttgctgacag
     3481 ctggtacaaa aggtaaacga tttgctttac caaatgctga gatcatgatc catcaacctc
     3541 ttggcggcgc tcaagggcaa gcaactgaaa tcgaaattgc ggcacgccat attttgcaaa
     3601 cacgcgaacg cttaaacaaa attttagctg aacgcactgg acaaccgctt gaagtgattg
     3661 aaaaagatac agatcgtgac aactatatga cagctgaaca agcaaaagca tatggattga
     3721 tcgatgaagt catggaaaac agttcttcat taaattaatc tgtttcaaag gaagcttttt
     3781 taagctctct tatctaaacc aaataaaaaa ggatggtcaa ctcaaactga ccatcctttt
     3841 ttattttatg gatgctactg cctatctttg ctccttgaat tttccttgcg catgcatccg
     3901 acctatctcg acataatgct ggatgttttc tttgtttttt tgtatttctt cttttgtcaa
     3961 aggacggatc actttagctg ggcgaccaaa agccaagaca ttaggaggaa taacttttcc
     4021 ttctgttact aatgatccag caccaatcaa actattctca ccaataaccg catgattcaa
     4081 tatcgtggtc cccatcccga tcagcgcccc tttttcgatt gtacagccat gtagcatgca
     4141 ctgatgtcct accgtcacat tctccgcaat atctacaggt gcatcatgat cgacatggat
     4201 gattgtacca tcttggatat tcgttctttg tcctattttt atccaattac tgtccccgcg
     4261 taaaacagct tgataccaga tcgtcacatc ttctgacaat gtcacatcac caatcactgt
     4321 ggcatttgat gctataaacc gttccatatt tatttttctc ctttcatttt ttgtagaaac
     4381 taatcctatt gaataaaggt atcatagcat atatttattt cataagaaaa aaaacaggta
     4441 aaacctgctc aggccagatg tctatttaca gtatctggta actgacgttt tacctgtttt
     4501 tattgtacat ctatattttc ttctattact ttttttaaaa ttaattcgta cacttctatt
     4561 tgtgctgcca agcgttcgat ttgaaactca ttttcaaaaa aatcagctga atcaaaaggc
     4621 ttcgtctgac agacgtgcgt taaacgatca acttctcttt tggcttcagt aattttttca
     4681 tttaaaaact gttccatctc cgtgtcctca cttttcttga taactttact ttaccattat
     4741 caagaataga gaacaaaaaa taagttttaa actgttttat tcttatatga cttattctgc
     4801 ttcctcagca aattggctgt tatacagttt ttcataaaat ccaccttgtg ccaataaact
     4861 ctcatgcgtt cctttttcaa tgatctgacc ttgatccatc actagaatca gatctgcatc
     4921 acggattgtc gataaacggt gagcgatgac gaaacttgtc cgtccctcca tcacgcgatc
     4981 cattgctttt tgaatcaacg cttctaaacg ggtgtctact gaacttgttg cttcatctaa
     5041 aatcaagatt ttaggatcgg agaccactgc tcgggcaatc gtcagcaact gtttttgtcc
     5101 taaagacaca ttgtctccct cagaattgat ggccatatca tatccatctg gcatcgttcg
     5161 gataaaatga tctacgtttg ctgttttcgc agcatcaact acttcataat ctgtcgcatc
     5221 aagctttccg aagcggatat tatctgcaat cgttccttga taaagccatg catcttgcaa
     5281 gaccatacca aatagcgaac gcacatcact gcgactcatt gatttggtgt cgattccatc
     5341 aatccttatt gctcctttgt ctacgtcata aaaacgcatc aatagattga tcaatgtcgt
     5401 cttgccggca ccggtcggac cgaccacagc tacggtttga ccagctttca cttcaaaatt
     5461 caaatcttgg attaatggtt tcttcggatc atatgcaaaa tcgacatgat cgaatgtcac
     5521 atctcctaaa atcgtttctg gcagtgaagc atctactttg ttttcttttt cttctggctc
     5581 atctaaaatc tcgaatacac gtttcgttgc cgctgatgca ctttgcaaaa ctgaagacaa
     5641 ttgtgtcacg ttccccattg gctgactgat ttgccaaata tattggatga atgcttgtag
     5701 ttgaccgaca acgatcgccc cagtaataac ataaaagctt cctagaacag ccataccaat
     5761 ataagtagca taggcagtta attgaactag aggcatcatc aagccagaaa tgaatgctgc
     5821 acgaaaccca aagtatttca agctgtgatt aacttttttg aatccttcca atgtttcttt
     5881 ttcgcgtcca tatactttca atacactaaa acctgtcata ttttcttgga cgtagccgtt
     5941 tagctcacca agagaattct gcatgctttg aaaatatttc tgcgaagcat tgatcactcg
     6001 ttttgagatc cagatcgatg ttggaatcat gatcatagag atcagcgcca tcatcggctg
     6061 gatataaaac atcattgctg cagccaacgt aattcccaat atcgcattta cgataccaat
     6121 aaaagcttgt tgtaaagcac cgctgactgc atctacatca ttcgtcacac gagacaaaat
     6181 atttccttgc tggtttttat cgaaatatga aaccggaaga cgattgattt tttcttctat
     6241 gtctcttctc aaatcatgca tggcactttg aacgacattt gtcatcaaaa caccagaaag
     6301 taattgcgaa atacaatacc ctattccaac aactgtgatc caaatgagac atttgaatat
     6361 ataagaaaaa ttgattgatt cgccattggc aatattacgg ctgatttctg tagtaggaag
     6421 tcctgtaaca tatggcaaag ccacgttgaa agccactgtc agaactgtaa ataggatcac
     6481 taaaagaaaa gtctcacgat acggtctgat atatcgagct aaacgtttta aggaagaaaa
     6541 ggtttttttc atgctaattc ctcctttgat aattgagaag cggcaatatc ataataaatc
     6601 gggcattttt ccaacaactc ccgatgcgtc cccataccga cgacttctcc ttcatttaat
     6661 acgatgatct tatctgcatg catgatcgtt cccacacgtt gtgctacgat caatacagta
     6721 gattctgtcg tttctttttt cagacgagca cgtaacttcg catccgtttg ataatctaat
     6781 gcagaaaagc tgtcatcaaa aatatagatt tccggacggc gtatgatggc tctggctatt
     6841 gccaatcgtt gtttttgtcc acctgaaaaa ttggaaccac cttctgataa atgttcatta
     6901 aatccatctg gttttcgaga aatgaattct gaagcttgag caatctcagc tgcacgttcc
     6961 atttcttctt gcgtagcatc ttctttcccg tatcgcaaat tatctgcaat cgttccagta
     7021 aatagcagcg ctttttgcgg aataaaccca atcttatctc gcaacttgct tagccgataa
     7081 tctctcacgt ctactccatc gatcaagatc tttccttcac tcacatcata aaagcgaggg
     7141 atcaattgga tcaacgtaga ttttccgcta ccggtacttc caataaaagc aacggtctct
     7201 cctggagagg ctgtaaaact gacatttcgt atcactggct cttgcgaatg gcctggataa
     7261 gcaaatgtta cattttgaaa ttcgatatac cctttagttt ttgtatcaga tataccattt
     7321 ggatcttcta caatcgaagg tttggcatct agggcttctt ggatacgtcc agctgaaaca
     7381 gctgctcttg gatacatcat gaacacacta gcaaataaca tgaatgagaa caaggcatgg
     7441 aatatatact cgataaacgc aatcaaatct cctacacgaa gctcgcctgc atcgatttgc
     7501 actgccccgc tccagatgat caataccatt acgatattaa ataagaaata aaaacccggt
     7561 tgtgcaacag ccatcaaacg gaataagctt tttgaactag aagcataatt ctcattgact
     7621 acagcaaaac gtttttcttc aaacttttca ttgacgaatg cacgaatcac acgcaatcct
     7681 gaaagatttt cccgcaagat actgttgatt ttatctaagt ttttttgttg ctttttagat
     7741 aacggttcag acaccttagc aatcgctacg actgccaata atagtaaagg taatgcacct
     7801 actacataca aactgagtgt cacacttgta cgcatgacca tgtacaaact gacaatgaac
     7861 atcattggtg ccataaaacc gatacgtaaa atattttgca ggaacaacat gatctgataa
     7921 gcatcgtttg ttgtacgagt gatcaatgaa gatacgccta gattttcgta ttcctgatgc
     7981 gaataacttt ggatcttttc gaacaaatca tctcgaatat ctgctacgat attagtcgta
     8041 atcctcgaac caaaataacc taacaagata ttcaaaataa taccgattac agtaataacg
     8101 atcataaata tcccttgttg cttcacataa tcaaaatcat cattgcggat tcctacatcg
     8161 atcatacgtg ctaaaatcgt aggaagtcct aattcaatca aaataaaacc aaatacgcat
     8221 atgaaattga aaaacaagaa tttcttataa cgcatggtat aatgccacat tagtttcact
     8281 ttctcatctc cttttaatct tttgttacat tttcattact aaaaatgata gcacaacgag
     8341 cctattaata gaacacttct ctcataaaaa agtcattatg attatagcgg gatgataaac
     8401 attgattttt tctttcaatc tagttgattt ctatacttat ttgctgtttt atgctagaat
     8461 aacaattaat tgttgtatat tttttataca gaaggtacgg ttgaaaaaat atccgaacag
     8521 ataagcaaaa acgaggcttg tctctttgac tcgaatccag tattctagtc aaagcatatg
     8581 aaaaaccagg tggcttgtcc cctcgttttg tgttgtctaa aaaaatcaac tagctaaatc
     8641 actgctttac gcagataaag gagacaaaac aatgatctat aaagtttatt atcaagaaac
     8701 aaaagtacgc aaccctaaac gggaagaaac aaaatctctt tacattgagg caaaaagcga
     8761 tgtagacgct cgccaacgtg tggaagaaaa taccccatac aatattgaat tcgttcaact
     8821 tctagaagga agtcatctag agtacgaaaa agaaaatgct gatttctcat tagtggagtt
     8881 ttaatatgaa actttccatc aaaaataacg agacaggcgt ctttgctatt ggtggattag
     8941 gtgaaatcgg gaaaaatacg tatggggtac aattccaaga tgaaatcatt ttgattgatg
     9001 ccgggatcaa attcccggaa gacgatttac ttggaattga ctatgtcatt cccgactaca
     9061 gctacatcat acaaaactta cacaaagtaa aagccttggt gattactcac gggcacgaag
     9121 accacatcgg cggaataccc tacttgctga aacaagcaaa tattcctatc tatgctggtc
     9181 ctctcgcatt agcgttgatc acaaacaaat tagatgagca tggcttgctt cgagatgcag
     9241 agctccatga gatcaatgaa gatacgatca tccgttttag aaagacttct gtcagctttt
     9301 tcagaacgac ccatagtatc ccagatgcat tagggatcgt ggtgaaaaca ccttctggaa
     9361 atatcgtagc gactggagac ttcaaattcg atttcactcc tgttggcgaa ccagcaaact
     9421 tgcataaaat ggctcgtatc ggggaagaag gagtcttgtg ccttttatct gatagtacaa
     9481 atgctgagat tccaacattt actaaatcag aaaaaacgat tgggcattct attttgaaga
     9541 tcttttcaaa aatcgaagga cgtatcattt ttgccagctt tgcttctaac atcttccgtt
     9601 tgcagcaagc agcagatgcc gctgtggcaa ctggtcgtaa aatcgctgtt ttcggacgaa
     9661 gcatggaaaa tgccatcgtc aacggtcaac gtttaggata tatcaaagtt ccagacggca
     9721 catttgtaga tgcacacgaa atcaatcgct tgcctgccaa tgaaacgatg atcctatgta
     9781 caggttctca aggagaagcg atggccgcgt tgagccgcat cgcgaatggt actcaccgtc
     9841 aaatccaaat ccagccagac gatacagtga tcttttcaag ttcacctatt cctggtaata
     9901 caacaagtgt caatcatttg atcaatttgc tgtctgaagc tggtgcagaa gtgattcacg
     9961 gaaaaatcaa taacatccat acttccggtc acggtggaca agaagaacaa aaattaatgc
    10021 ttcgtttgat gaagccaaaa tattttattc ctgtccacgg agaattccgc atgttgaaag
    10081 tacatgcttc tctagcacaa gatgtaggtg ttcctgcaga aaactgcttc atcatggaaa
    10141 atggcgatgt tttagcgtta actagcgatt ctgcgcgtcc tgctgggcat tttgtggctg
    10201 gagatgtgta tatcgatggt agcgggatcg gtgatattgg taatgtcgtt ttacgtgacc
    10261 gtcacctgct ttcagaagaa ggtcttgtat tagctgtcgc gacagtggat atcaagaaaa
    10321 aagaaatctt ggctggtcca gatattctct ctcgtggatt tgtctacatg cgagaatcag
    10381 gagaaatgat caatgaagct cagcgaatct tattccacgc tttaagagaa tgtttgaatg
    10441 gacctgactg ttcagaatat aaattgaaag aagcgatgac tggcgcgctt cagcctttct
    10501 tattcgaacg tacagaaaga catccaatga ttttgccaat ggtaatgaca ccagatgaag
    10561 aataaataag aggttatgaa atcagcacct tgtcctgaat ataagagaaa acaaatgaaa
    10621 aacatgagaa gccatttttg tttgttctta ttccttgata caaggtggct ctttcatagc
    10681 ctcttaaatt tatttacttt ttcatacaaa aaaatacctg ccacaagaga aattgtctct
    10741 tgtggcaggt atttttgtta ctcgctttgt tctctcgtaa aaattgatgt ctaccatcaa
    10801 tttgatttac agataaaatt aagcttttac aacgtttgca gcttgtgggc cgcggtctga
    10861 ttcttcaacg tcgaaagtca ctgcttgacc ttcttctaat gttttgaatc cgtcaccttg
    10921 gatagctgag aagtgaacga atacgtcgct tccatcttca cgtgagataa atccaaatcc
    10981 tttttcagcg ttgaaccatt ttactgttcc gttttccata ataatatcct cctcgtgcca
    11041 aaagcactaa tttaaattgt aacattgctc gtgtacgata gaggtgcaac agatgtttca
    11101 acttctattc ccaaacaaaa ttacaatata attgtacact tgcttcattt tttttgcaag
    11161 aaagcaactt acttctcttc catcattcct tcttctctca aactaaaata tcgtttgcgt
    11221 ccgatgatca agtggtccag tacagtgatc cccatcattt ctccacactt ttgtatcctt
    11281 ttggtaaaat tcatatcctg ctgcgatgga ataacattac cagaaggata atataggtta
    11341 gtacacgtat aaaaatatct tttatattga cacaatttat ttaacaagta taataataat
    11401 tgaaagtgaa tttaaacaaa tatacaccat aaacacaacg tacctgcgaa tacgttgtgt
    11461 ttttttcttt ttcaaaaaaa gacgcttacc gctgcgaaac agtaagcgcc attgtcggct
    11521 ctaatgttta tctagccaat gactaaataa tgccaaagtt aaaccaatta acaatggcaa
    11581 gagcacgtgc tccaaagtga attcaaacaa atcatacacc tcctcccacg aaaatctctg
    11641 tggaagcgac gagccgacat tttattatac cattattgaa ttttattttt tatcaaatag
    11701 attaaattat gtaccctgta ggactcgaac ctacgaccgg acggttatga gccgtctgct
    11761 ctgaccaact gagctaagag tacaggttgt tgccacataa agccataaac aatcaaccag
    11821 tagaaggtgt ggcaacaatc ctgttatcgc atagcttgga gtgtgactat tatgggtgat
    11881 agtgaagata tgcga
//
LOCUS       DACEXQ010000070        11874 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.070, whole genome shotgun sequence.
ACCESSION   DACEXQ010000070 DACEXQ010000000
VERSION     DACEXQ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.070"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(214..1230)
                     /gene="tsaD"
                     /locus_tag="IXH04_001334"
     CDS             complement(214..1230)
                     /gene="tsaD"
                     /locus_tag="IXH04_001334"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAQ1851952.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(1227..1691)
                     /gene="rimI"
                     /locus_tag="IXH04_001335"
     CDS             complement(1227..1691)
                     /gene="rimI"
                     /locus_tag="IXH04_001335"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ1851953.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(1698..2240)
                     /gene="rimI"
                     /locus_tag="IXH04_001336"
     CDS             complement(1698..2240)
                     /gene="rimI"
                     /locus_tag="IXH04_001336"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ1851954.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LGVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(2224..3048)
                     /gene="tsaB"
                     /locus_tag="IXH04_001337"
     CDS             complement(2224..3048)
                     /gene="tsaB"
                     /locus_tag="IXH04_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAQ1851955.1"
                     /translation="MYYCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDNIIVPMFDARRKNVYAGAY
                     RWKENRLETVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFSEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            complement(3137..4117)
                     /locus_tag="IXH04_001338"
     CDS             complement(3137..4117)
                     /locus_tag="IXH04_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ1851956.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(4141..5625)
                     /gene="galT"
                     /locus_tag="IXH04_001339"
     CDS             complement(4141..5625)
                     /gene="galT"
                     /locus_tag="IXH04_001339"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAQ1851957.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(5637..6626)
                     /gene="galE"
                     /locus_tag="IXH04_001340"
     CDS             complement(5637..6626)
                     /gene="galE"
                     /locus_tag="IXH04_001340"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAQ1851958.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            6874..7041
                     /locus_tag="IXH04_001341"
     CDS             6874..7041
                     /locus_tag="IXH04_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAQ1851959.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(7103..8914)
                     /locus_tag="IXH04_001342"
     CDS             complement(7103..8914)
                     /locus_tag="IXH04_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAQ1851960.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(8911..9276)
                     /locus_tag="IXH04_001343"
     CDS             complement(8911..9276)
                     /locus_tag="IXH04_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAQ1851961.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(9439..9834)
                     /locus_tag="IXH04_001344"
     CDS             complement(9439..9834)
                     /locus_tag="IXH04_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ1851962.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(9852..10814)
                     /locus_tag="IXH04_001345"
     CDS             complement(9852..10814)
                     /locus_tag="IXH04_001345"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAQ1851963.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(10814..11026)
                     /locus_tag="IXH04_001346"
     CDS             complement(10814..11026)
                     /locus_tag="IXH04_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAQ1851964.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(11047..11745)
                     /locus_tag="IXH04_001347"
     CDS             complement(11047..11745)
                     /locus_tag="IXH04_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAQ1851965.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(11765..>11874)
                     /locus_tag="IXH04_001348"
     CDS             complement(11765..>11874)
                     /locus_tag="IXH04_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAQ1851966.1"
                     /translation="NNFHKKTCSLVFREQLNEQWQPESFDQKIQQLFID"
BASE COUNT         3338 a         2609 c         2032 g         3895 t
ORIGIN      
        1 tcacaagtca taatgttcat aaacatgagg agctgttttt tgaaattgtt ccttgtgaat
       61 cagagttaaa cgacaggctt acgaactctt gattaaacgg cgttcaaaag cttgcatacg
      121 cggtaataag aacagccagg tcaaaaacat gagaagccgt ttttcctggg ctgttcttat
      181 tacttgatgc agggcagctt tttcacaacc tctttattca gataaaacca agcttggatc
      241 agcattcaac tgatagctag caaaatttct tttttgcatt tccacatgtg ctgctgctcc
      301 aatcattgca gcattatctc cacaaagtcg aagaggggga atcactagtt ctacctcagg
      361 gagtttagtt gccaaagcat cctgcaatcc ttcacgcaac ccttgattgg ccgctacacc
      421 tccagctata atcagttgct tgactggata attttgacaa gctcgaagtg ttttagtaat
      481 caatacatcg atcacacttg cttgaaagct agctgctaaa tcatttttgt ctagatcttc
      541 tccacgctgt tgtgcattat gcaccaaatt gataaatgca ctttttaatc cgctaaaact
      601 gaaatcataa ttatcttcat ggatcatcgc tcgcggaaaa tggtaattat cttttccctg
      661 atgagctagc tgatcgattt cttttccgct tgggtaagaa agacctaata ctcttccaac
      721 tttgtcataa gcttctcctg ctgcgtcatc tcttgtctcc ccaatgattt catatgatcc
      781 atcttcttgc atatagacta attctgtatg tcctccgcta acaagaagtg ccatcaatgg
      841 aaactggaat ggtttaacca gacgggctgc atagatatgc cctgccatat ggtttacagg
      901 aataagcggc aattggtgtg cccaagcaaa agccttagct gcagaaatac caatcagcaa
      961 cgagccgaca agtcctggtc cataagtgac agcgactgcc gataaatctt ctgccgacac
     1021 accagcttct accagtgcat cttccaagca taaggtgatt tgttctacgt gatggcggct
     1081 agctacttca ggtactacgc caccaaaacg tttatgactg tttatttgag aagcaacgat
     1141 gttagataag atctctgttc catttctaac gaccgctaca ctcgtttcat cacaacttga
     1201 ttcaatcgcc agaatcaatt cttcattcat ttcatcgtct ccgtttttaa ctttctgctc
     1261 ataatgatcg catcttccgt cggattgcga taatatgatt tccgtttacc taatatacga
     1321 aacttttctg attcataaag ttttcttgct gcttcattag atagacgtac ttctaaaaaa
     1381 acatgcagcg aatcttttat ttgttccgta atcagtaatt gtaacaaatg ccgtgcatgc
     1441 ccttttcctt gttctgaaac tgcaacagca atatttgtga tctcgacttc atctattact
     1501 ttcgagtatc caataaagcc acagatctgg ttatcttcag ctaaaagcag ataatcagag
     1561 tgaggctgtt ggatgtctgc caaaaactgc attttcgtcc aaggtgaacc atgtggataa
     1621 gcattttcgc taatcatcca tacgcattct gccaagtacg cttcatcaaa atcttttttt
     1681 ctgcaaatca actaatctca ctcattttct aaaagtttta tcatatccaa cgcatcttct
     1741 tttgtttcag tgtaatatct ttttttgatc gttgttgctt gaaaccccaa tttccgataa
     1801 agtctttgtg catcttgatt actcatacgc acttctaatg acatcgtctc acagcgattc
     1861 attatagcaa agttctcgat ttcgtcaata agcagcgaac cgattcgttt acgctgatat
     1921 gccggtcgaa cggcaatatt cgtgatatgg caatctaaac cgaacgctct tgaaccgata
     1981 aaaccaatca ttcttccatt atcaaagata cctagatata gatgaggaag cggtgacttc
     2041 aattctgata taaaggcact ttttgtccat ggcaattcgc cattatagac ttctcgttcg
     2101 acacccagca gctctttgat atcttctgta gtcaattctc ttagtacgta acgacgatcg
     2161 atttgttcga ctgttttttc tgtataaatc attttatctt gaaaaaagct tctggcatac
     2221 gctctaaatt ttttcaacat aactttcgtc tccaggtgtg tgcgtctcca accatttttc
     2281 ttctgcttct actcgtttta aatagcgagg aagaaaagct tgtttctctg caactggttc
     2341 aactaaagca cctaattgag ccacagttac accacttgga atatcccatg ctggaactgt
     2401 attaatcatc gcactaggca atatttcctt tatttcagag aagaatttgt gacaatcact
     2461 cccgacgaaa taaatagctt cattcagctt ttgtagttgt gtgaacaaat cagaagcaga
     2521 aatatgtaca tcttcaagta cagtttctaa tctattttct ttccagcggt atgccccagc
     2581 gtatacattt tttcttcgag catcaaacat gggcacaata atattatcca caccgatgca
     2641 attagccgcg atggttttca gactagatac tcctactagc tctttatcta atgtataagc
     2701 aatcgttttt gctgtcgtta cacctatacg aagccctgta taagaaccgg gaccatcaga
     2761 aaccgcgatt cggtcaatgt cttttggact tatttgaaga tctgcaaaca attggtcgat
     2821 tgcaggcatc agtgttacac tgtggttgcg tttgatattt gtttgtattt gtccaagaat
     2881 ttgttcgtct tctaccacac cgatcgccaa agtttggttt gcagtatcta ttcctaatgt
     2941 aatcatatat ttgatccttt ctgctatgac attctcgcct tccattgtag catacttttt
     3001 tattctgagg atgaaaatac caaaggcaaa actagtacaa taatacatga ttatctgctg
     3061 gcgggcaaat tttgcccctt cctgaaacac aaaaaagctg cggtatcact ccattttccc
     3121 acagcttttc catcttttat ttcgttgatt ctcgaataat caactctgtt tccacaataa
     3181 tctttcttga aaccggtgct ttggaattac ttaattgaag aatcgtctcc actgctagct
     3241 ctcccatcca ttcggtataa acttttacag tagaaagcga gggcgttaca tatttcgcta
     3301 cactgacatc attgaagccc atcacagaaa tatcttctgg taccactaaa cctttttctt
     3361 gaatcgcttt cagtgcgcca atcgctaatg catcattagc tgcaaaaagc gcatcaggaa
     3421 catgcggatt cgtttttaaa tactgcttca ctgcttgata tcctgactcg acagaaaaat
     3481 ctgcttcgat aattggacct tcgtcgtagc ctttttctgc caaaacttgt tgaaatgcca
     3541 taattctggg atcttttagc cgctgatggt ttttcttcgt atattctgtt ccagcaagca
     3601 tagcgatatg ttgcgcttta tgggagacaa agtgttcaat gactttttga acacttgact
     3661 taaagtcgac aactagagag tcgattcctt gagaagtgcc gtcagaatct accaaaagca
     3721 aggcattttg ggatcctttc atttctaata actgagcttc gtcgaatttt cctagtgcga
     3781 tcgttccatc tgccggaaga tcactaatgg aatcccacgt ttctttcaat aaagtaacgt
     3841 ttgcttcttc tgcctttttt tcgattccta ggcgaataga taaataataa agatcttcaa
     3901 gctcttccgt atcgtcgtac cactggatca ggcgaagcgt ttgcttagta tttgctgcat
     3961 ttttacgatg tttcgtataa tttaagcgtt ctgctgcttc aaaaattttt tgctttgtgg
     4021 ctaagccaac ggaaagatca ggatcatagt tgagaactct tgaaacagta gcaggagaaa
     4081 ctcctgccaa ctgcgcaatg tctttaattg ttgccatttg tctctcctcc ttttttacgt
     4141 ttataaaatc gcagtaaatc gttcaaacgc tgcctgtcct ttttcatctc gcttgaatac
     4201 tccagcgtct tccagtacac gtgcaaaaac ctgacctaca gatttttgta cgacatcggt
     4261 gaccgtatct ttcgtcaaat caggatgttc tttttttaat tgttctgccc acggacgatg
     4321 atattcagcc atctggtttt cttctcctaa taagaaatgc tctacttcta ctagttctgg
     4381 tttcaaacga ggtggtaaaa cagcaagccc cattacttcg atcaacccaa tgttctcctt
     4441 tttgatatgt tggacatctt tatgtggatg aaaaatccca tcaggatgtt cttctgaaac
     4501 attgttgtct cgaaggacaa ggtccaactc aaagtaattg tcccttcttc gtgcaattgg
     4561 cgtaatcgta tggtgtggtg taccatctgc tgaaaaagct ctgatagata ctgtttcatc
     4621 cgaatatgct ttccattttt ccaatactga gttagctgct tcaataagca agtttttctc
     4681 tgttgattgc aaacggatca cagacatcgg ccatttcacg actcctgcga tgacttgcgg
     4741 ataatcttct agtttgataa gtttttctat cggtgctttt gccataggaa attcatgacg
     4801 tcctgcctga taatgatcat gagaaagaat cgatcctcca acaattggta agtcagcatt
     4861 cgaaccaaca aagtaatgag gcaagacttc agtaatccga agcagtctag caaatgtttc
     4921 ctttgtgatt ttcatcgggc ggtgttgttc tgataaaatg atcgaatgtt cattgtaata
     4981 agcataaggg gaatattgga atccccagct ttcaccgtct agattcattc gaataatccg
     5041 gtgattggtg cgggctggat agttcaagcg acctctatat ccttcgtttt ccatgcaaag
     5101 catgcaggta ggatagtcta cttttaccgc ctcgcgttct gctgcgattt gttttggatc
     5161 tttttctggc ttagataaat tgattgtgat ttccagttca ccatactccg actctgccgg
     5221 aaatttgatg ttttttgcga tggctcttgt tttgatgtag tcatttttct tacttaaatt
     5281 aaagaaatac tcagttgctt cctctgggct tttcgaatag tgctgtgcaa agaatgcatt
     5341 gacgactgaa ggtggcggtg tgagaaaatc cattaattgc gcttcaaaaa tctcttgttc
     5401 tgctggcaat tctgaaagaa caccattttc tcttgcctta gcgacaagct gatccaataa
     5461 gtctacagat tcttccttaa ctggtcttac ttcaatgctt tctagagcat cttctcctac
     5521 catccctaaa agacgattct gtaaatataa gcggtccatt tccatccatc cgccagcttc
     5581 gatcgcaagt gtggcaaaat cacaaatcgt ttgactgatt gacatatctc attcccctat
     5641 tcttcataac catttggatg gctgacatgc caatcccatg ctgttttgat aatcgctttg
     5701 atatctgtgt attcaggttt ccagcccaga atctctctcg ctttttcact tgaagcgact
     5761 aatcggctag gatctccagc tcttcttggt gctatttctg ctggaatttc ttttcctgtc
     5821 acttctctag ctgcttctag catttccttg actgagtatc ctttgctgct gcctaaattg
     5881 aagaagttgc tttcatttcc ttctttcaag tattccaaag ctaaaatatg tgcagcaatc
     5941 aaatcttcta cttgaacata atctctgata catgttccat ctggtgtatc ataatcgtct
     6001 ccataaacag ccaaggcttt tctttgacca agagctactt gtaaaatgat tgggacaaga
     6061 tgcgtttctg gtgtatgatc ttctccgata gatgcatctg cttttgctcc ggctacattg
     6121 aagtagcgca aagcgacata gcgcatgcca tatgcttggt cacaccactt catcattttt
     6181 tccatcatca atttactttc accatacgga tttttaggat tagctggtgt atcttctgtg
     6241 attggagact ctttcggctc tccataagtt gctgcagtag aagagaatac gattttattc
     6301 acgttgaact cgtgcatgac ttcaagtaag atttgcatac cgtatacgtt gttgttgaaa
     6361 tacatcaatg gtttttcgac agattctcct actaaagagc ttgcagcaaa atggattact
     6421 ccttcaatcg gttccttttc aaatacggaa cgtagaaatt ctttatcccg gatgtctcct
     6481 tcataaaaat gagcatctgg atgtactgct tgtttgtgac ctgtgagcaa attatctacc
     6541 acagctactt gatacccctt ttgaactagc tggtctacag cgtgcgaacc gatataaccg
     6601 gctccgccta gtactaaaat tgtcataaaa aacactcctt tgattagtaa aattgtgttt
     6661 gttgattcta tataatttaa aaaacgaagt ttacttttat ggtgaaaaca aacaaagtaa
     6721 aatttcgtaa attcatatgg taaatttata ataaaacatt tagtaaacat tttcaaggat
     6781 tttttgcaaa ggtttacaaa caaacgtaaa actagccttt ttttctcttt tgttccataa
     6841 tagatataac aaataataaa tgaggtggat tcaatgaaaa aatttgtatc tggaatatta
     6901 gtaggaagtc tggcaacagc cgctgccgtt gctggtttgg ttgcttctgt gaaaaaaaca
     6961 gtgatcgatc caattgacga aaaagaagca atgatcgaag aaaaccgtaa aaaagcaatg
     7021 cgtaaacgcg tttctcgtta ataattattt aaaaatacgc atgaagactt tctaaaaaac
     7081 agataagtca tcatgcgtat ttttatttgt cagtcaatat aagttgtgct attttatctg
     7141 cctgtctcag attccatgga tctgtataag gtctagctac tgctgcagtt agccaatcca
     7201 caccttcaac aggtattcca tagcaataaa agtcagactg gatttcccct gaagcatcaa
     7261 tcacgcgatt cgtacctcgc tcaacatcta tagcttctga atgataactt cctgtattat
     7321 ctgaaaatga aaaagggcga acaagtttgt tttccttcaa attttttatg acaggattac
     7381 ttgaacgaga aaagcttgtt tgtggcaatc gagcttcaat caaatagcga ctgctgattt
     7441 ctttatcttg tagagaaata acatagcggt tgtgcttttg ctgaatatca aatggcggtt
     7501 cgatcaaatg aaaaataccc gcatctatca gtgctgctaa ttctttcgtc cgaatgattg
     7561 gcggaccgat cgttaaaaag ctatttagat gagtaaacca tccccatagt atttctttgt
     7621 attccttagc agaaaaaacg tgccattcaa tagcctgccg gattggatca cgccagtctt
     7681 ttaatgcatc aaatgttgaa gcaaatgctc ctgtgcaatt gcctttttcc gcttctttta
     7741 tctgcccttc aataaatatt cggctagctt ctgaaaaggg ctcattctga taggcaagag
     7801 gagtttctaa atacgaccaa tcccatctat aagggattag tgctggatgc tttccgagac
     7861 actcctctga agagagggag agaaaatcat gctcaaaatt tttctttgga atcggcaagt
     7921 gatattcttc gatgattttc ttgtaataaa acaactcaac gtcttttttc agatagtcaa
     7981 aaaatgtttc gccagtcaaa aatcccgact gatgccattg ctccaggttt tcttttgtta
     8041 gtaaccgtgg ccatgccatt gctccgcctt gcttttgatt tcttcctcga gggtaataag
     8101 ggagccccct tcttgatcca ctgtatacaa tcggttcctt acctgatgga tgatagacca
     8161 gttttccctt ttcgttctcg aagcgccctc ctcttgatac tgtaaacaat ccgacataat
     8221 caaaaaaact taagcctaga cctcttaaaa agacattttc tttaggcggt atcttcgaaa
     8281 gatctacatc tgcaggatta gaaggaagct gatacagtaa aacatttttc tttgcgtagt
     8341 ctacccacac tctttcttct tctgtctcat cattttccca gtgaccgctt gctaaaacaa
     8401 gctgatcgat agaatagctt tgctgatctg tctgtaaaag ataaccatct tttcttttgc
     8461 ttactttttt tactaaagaa taaacaaact gaatagaagc tggaaattct tcttgcaact
     8521 ttgagaaaaa ccagcgttga tagagtccgt ataaacatcg agtagattgc tgatcttttt
     8581 ccagacgatt gatttcttct ataattagtt gcttattttt ggttaccgtc tgttcgatat
     8641 aatctgtagc aggtccttta ctccattgaa acaagttggg tccctgtgtt accaatcctc
     8701 cactggacaa tgtttcatct gtaaaaagcg tcacttgttg agaaacagaa ttcattaata
     8761 actccggtgg ctgattcaac cgccaaactc gacctccagg accattggga tcgaaaagaa
     8821 aaagttcaag agaatgtctg tcctgattat tatggagaat cctttctgct acacttaatc
     8881 ctctaggtcc ggcaccaatg atccctattt tcatttgcat gcctccattt cgataaattt
     8941 tttcaataaa acagcatggt tctgatgttc attttttgca ccataaacaa aagtaatgac
     9001 tggatgttct ttcataagtt gactgagttt aaaaatagct tgcttctttt cttcttcgaa
     9061 aatcaattct tgctggtatt tctttgcaaa tgaagcaaat cgttctggta catggtcaaa
     9121 agttttgcgt gcttcattac taggtgcaat ttctttcaac cacagatcta actgcgcttt
     9181 ttcttttgaa attcctcgtg gccagaatct atccacgagt attctatagc catcctcttt
     9241 ttcagcatca aggtaaacac gttttaattt gatcatagtt gtttccttct ttctaccttt
     9301 tcgcttagct tatatatgta gataatagca caaaataaaa aaaatgaacg gcacaactat
     9361 ccattgcgcc gttcatgtga tcttggtcaa aatctaatga ttgattcggt ttttaaagct
     9421 gtcatacaat catctgtttt atactttttt acttttgatt tttccagacc atccgtcata
     9481 tccaccctta agtaaataaa tctcttgata acctgcttta cgtaatttat ttgctgtacg
     9541 gatgctcaaa cttttctttt gatcgtaaat atagacaggc tggtcttttc gaatagatcc
     9601 taatgaatct ttcaaaacag tgtaagggat gtttcttgca cctaaaatat gcccagcatc
     9661 aaacacatct ttttcgcgaa catcgattac ttgtgctttt ctcattcctt ctcgaaattc
     9721 ttcttctgtc aaagtcgttg ctgaacgttt ggccattact cgtagataga cttcccaaag
     9781 gatgattgca agcacaatca atagcaaaat gatattaatt acccataaaa ccatgtaatg
     9841 cctccgtttt tttaagcaaa tttcaaggct aatgaggcag caccaatgac accagcttca
     9901 ttaccaagct ctgctaattt gatttttgtt gattctctta cttgcgggaa agtaaattct
     9961 ttaaaatatt tctctacacg gctacgtaaa aattctcctg ctgccgatac tcctccacct
    10021 aacacgatac ttgacggatt taatgtattc cccaagtttc cgcaagctaa acctaagtag
    10081 aagcatacgc gatcaacaac catcaatgca aacggatcgt cttcttgagc catttcaaag
    10141 atatctttac ttgttacttc ttctccgttg tctagcattg ctttcagttt tgaatcccct
    10201 gcgtattctt ctgccaagaa acgacctagg cgtaccacac ctgtagcgct agatacagtt
    10261 tctaaacatc cgcgtttacc gcaagtacat tcgaaccctt caggatccac agtgatatgt
    10321 ccgatttctc ctccagctcc gctcacgccg tgaatcaaat ttccgccagc aacgatacct
    10381 ccgccaacac ctgttcctag agtaacgaat acaacgtctg gatcgttttc tccagcacct
    10441 ttccatcgtt cacctaaagc agcaacattc gcgtcattat cgatagcgaa tttgattcct
    10501 gttcctgcct cgatcagttt tttcacaaat tgaacttctg tccagtttaa gttatacgca
    10561 ccaataaccg ttccagcttc gctgtccacg cttcctgggg tacccatgcc gattccgata
    10621 aagtcttcgg ctttcatatt atataaattc aaacgatgat tgattgattc aatgatttca
    10681 ggaacgatat gcttaccatc atccaaaata ttggtatcga tgctccattt ttgctgaatc
    10741 tctccttctg gtgttaaaat tgcaaatttt gctgttgttc cacctaaatc aatcccaata
    10801 atcttcttgt ccattacaaa tttcctttct ttcggctttc ttcaattcgg tgctctcttt
    10861 ttaatatgcg ccacgctgtc atgtatgtat catgatcaat caaacggcta tcatacagat
    10921 tacgcaattc gatagtcatt aattcaatgt catagatgcg tgcacccaca tagataaaga
    10981 tgccaaattt tttgaacagc tgctgtacat catataagct ctccatcttt aagcacttcc
    11041 ttcatattat acaagtaaac catatttttt taaacccagt aaaatacata ttacaaaaag
    11101 aaatacaaaa actagtgtag aaagaatcct ttggtgtatc gaatagcttg tctgttgttt
    11161 cggtagaccc aaaacattcc ctaacactaa tccgccaatt agcccaccga catgtcccca
    11221 aacatctaca gaggtatcca gaagaccaaa aacaaatgtc aacacaataa ataaaagaaa
    11281 atgtctgact agctgctgga tcgctgggtt atgtttaaaa tgaaacccta agataaacaa
    11341 cgcaccgaac aaaccgaata tcgaagtact tgcaccacca gacagaacat tcgcttcgtt
    11401 aaaagcaaag cttgctgtat ttcccattat cccgctgaac atataaatca agaaaaaacg
    11461 ccaatggcca tagattgcct cgatttgctg ccccataaaa tacagtatca gtgagttcat
    11521 tacgaaatgc atcagtccaa aatggatgaa gattggtgtc actaaacgcc accattcatt
    11581 gaagtgaaca agataaggac catacattcc ggcataataa gggatctcaa gtcgcggaaa
    11641 taattccatc atgaggaaga ccgctgtctg tatccctaaa aaagcatagg tccataacgg
    11701 tcgtcttaac catgcacgca attttatttg ctgttgataa ttcatgaaaa cctcccattt
    11761 cattttagtc aataaacaat tgttggatct tctgatcaaa gctctccggt tgccattgtt
    11821 cattcaattg ttcacgaaaa acaaggctac atgttttttt atgaaaatta ttta
//
LOCUS       DACEXQ010000071        11799 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.071, whole genome shotgun sequence.
ACCESSION   DACEXQ010000071 DACEXQ010000000
VERSION     DACEXQ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11799
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.071"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..159
                     /locus_tag="IXH04_001349"
     CDS             <1..159
                     /locus_tag="IXH04_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HAQ1851967.1"
                     /translation="QSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAA
                     LGEIETAQ"
     gene            330..617
                     /locus_tag="IXH04_001350"
     CDS             330..617
                     /locus_tag="IXH04_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851968.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            843..1286
                     /locus_tag="IXH04_001351"
     CDS             843..1286
                     /locus_tag="IXH04_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1851969.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(1292..2476)
                     /locus_tag="IXH04_001352"
     CDS             complement(1292..2476)
                     /locus_tag="IXH04_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAQ1851970.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            2744..3442
                     /locus_tag="IXH04_001353"
     CDS             2744..3442
                     /locus_tag="IXH04_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAQ1851971.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            3449..4126
                     /gene="nth"
                     /locus_tag="IXH04_001354"
     CDS             3449..4126
                     /gene="nth"
                     /locus_tag="IXH04_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAQ1851972.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(4252..4785)
                     /locus_tag="IXH04_001355"
     CDS             complement(4252..4785)
                     /locus_tag="IXH04_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851973.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(5082..7463)
                     /locus_tag="IXH04_001356"
     CDS             complement(5082..7463)
                     /locus_tag="IXH04_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ1851974.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(7453..8100)
                     /gene="recU"
                     /locus_tag="IXH04_001357"
     CDS             complement(7453..8100)
                     /gene="recU"
                     /locus_tag="IXH04_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAQ1851975.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            8164..8703
                     /locus_tag="IXH04_001358"
     CDS             8164..8703
                     /locus_tag="IXH04_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAQ1851976.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            8793..9212
                     /gene="gpsB"
                     /locus_tag="IXH04_001359"
     CDS             8793..9212
                     /gene="gpsB"
                     /locus_tag="IXH04_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAQ1851977.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            9234..9601
                     /gene="rnpB"
                     /locus_tag="IXH04_001360"
     ncRNA           9234..9601
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IXH04_001360"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            9810..10976
                     /locus_tag="IXH04_001361"
     CDS             9810..10976
                     /locus_tag="IXH04_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAQ1851978.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            11022..>11799
                     /locus_tag="IXH04_001362"
     CDS             11022..>11799
                     /locus_tag="IXH04_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAQ1851979.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFG"
BASE COUNT         3974 a         2066 c         2416 g         3343 t
ORIGIN      
        1 caatcagcaa ataccatgtc tttagctgtt caaaataaaa tgactgtaga agatctcgca
       61 ctttcagatt tcttcttcca accacatttt gaccgcccat ggaattactt gaacctactt
      121 gcacaagcag cgctaggcga aatcgaaact gcacaataaa gaaaaagggt tgtgacacaa
      181 ttttgtcacg accctctgtc tttttttgtg aaaagtacaa ttagttgtct ttcttcgaat
      241 tttttgtcac aataagcagc aaagataaag ctgttacata aaagaacaag cagctttatt
      301 tgattttgaa aagaaaggtg agaaagaaga tgaataaaga atattttgtc caacaagatg
      361 catttttaga agattataaa agtgcttcga agaaattaac gatcaatatt gatgagttgg
      421 cagatcaagt actctctcag ttgaatgaaa cgcaagagga atattacacg atgccagcaa
      481 atgacgccaa agataatgtt tctcatcgtt ttcctttcag tagacgcatc tactcagatg
      541 aaaatgacgg cacattgaat acagagtaca catatgaagg tcagccatat gtgctagaaa
      601 ctgatgatga tctttaaaga cagtttttga aaaggccgct catccttaag tgatgacaaa
      661 aaatcatcac aattaccgat gactgtctct ttactatctt ttaatcagaa aaaggggtta
      721 tgacaagctt tgtcaacccc tttttttctt ggtttcagat tgggttttcc tacaaaatat
      781 ttttatcttt gttgaatagg aaacaaaaaa acaatataat gaaagaaaaa gaaggtgtag
      841 aaatggataa aagacaactg ctaatagaag aatatctgga tttagctaat gagattattc
      901 atcgtaataa accagaaatg gaaaaagaat ttgcaggatt tactctaaat gaactagaag
      961 tgattgaaca tatcgggaaa atccctgatc ctaacgtgac aaaattagcg aatgctagtt
     1021 acatgactag aggagccatc agtaagctaa caaaaaaact gatagctaga gggatcataa
     1081 acacttatca aagcgaagag aataaaaaag aaatctattt tcaattgacg ccaatcggac
     1141 aaaaaataag gaatagacac gagcaactcc atcaaaaatg ggtacagcga gatgctactg
     1201 tatttgaaaa tatgtcaaaa gaagaatttg acacagtatt tcgttttatc ggtcgataca
     1261 gagaatttat acgaagtagc aaatgaagct actatttttt attgtagtga taataggcaa
     1321 aaatcataag caaaaatccg aataccgtcg gaagaaatgg tgctgcagca taaagctgtc
     1381 ctgcgattag cgggccgatg acgcgcgata gagattgtat actttgggta ccgcccatta
     1441 caagaccttg ctggttatct gagacagaat tggatagttg gccattgaat acaggagtaa
     1501 aaatagaatc accgaaacca taacagataa tcccaaataa aaacaaaatc ggcagtagaa
     1561 gaattccaga taatgtgatg aatagatagg cgaggatttc actgaacatc cctattcttg
     1621 tgatctgctg ttctgaaaat tttttgagca atcgaggcat aatgaacaat tgcgagacag
     1681 tatccaagat tccaatcaaa gagaagctta aaccaaccaa cccagccttc cactgaaagg
     1741 cgtcaatcga aaattgagaa aagatagatt gaagagagcc agctgccaac caaacagcaa
     1801 aacctgctgt caataaagga ataacggatg aaatcttgaa taattgttgt aattggtgaa
     1861 aaggtcttac atgggagaaa cttaaatcta ctgaacgttt ttttattgga aggctttcta
     1921 gcataaatgt gtagccataa acggcattta agaaagtaaa gagtgctcca ataaatatag
     1981 gaacagaatt ccccaactca gctaatagac cccctataat aggacccagc gttgttccta
     2041 taccgactaa agcacccatc catccaaaaa ctttggttct ttcattaggt tccgtgatgt
     2101 cggcaaagta agcatataag gtggagattt ctcctgccgt caatccttca atgatacgac
     2161 caaggaacaa catccatatc gaatttccta aaccaaaaag caaatatccg atacttgatc
     2221 ctaataaact gataataagg atcggatgac gcccaaaatg atcacttaag ctaccgagaa
     2281 tgggggcaga taagaaggca gctagagcat aaacagacat gagcaaagtg atatagaaag
     2341 cttgctgatg tatggagtga gaataaggca gcgttaaaaa cggaagtaca ggactgacga
     2401 tagtaaatcc taatccagtg aataagacag agacgtatcc aaaaacttta gaatgtttat
     2461 ttgtgaaact gtacatgtaa aaactccttt atgcaataat gttaacttgg aaacaaaaat
     2521 agtgtatagt aattttgttt ccttgtcaac attctaagga acagcgaaat ctacttaatt
     2581 tatttattcc gaaaagtgaa gatacgcatc aaaaacatga acaaacaaaa atcaagtaga
     2641 cgacattgta aatctgaccg atcagtgaag taaatagaaa agaaacctgt tcttgcttcc
     2701 catgtttttt ttacgtatac tgtgattgag cggaggagaa atcatgttga atttagaaaa
     2761 ttacttaagc gctggacaaa cagtcatatc taatctttta cttaaaaatt atcataggat
     2821 cggtctacaa tctgaagaat ttttatttgt tctacagttg catatggcac agcttgaagg
     2881 agatactttc cctgatttgc agatgattgc tatggatatg ggaatcaaac aagatcagat
     2941 ttttcaaata ctggatcgtc tcgtaacaaa tggatttatc aagatagaaa cgacgattgt
     3001 caacggtaaa aaagcagatc gatataatct ttaccccatc tatgatctat taggtgagta
     3061 tttaaaaaca caagaaaaaa aatacgaaga aaaaacacaa gaaaaagaaa tccagtcagt
     3121 gtatcaatta tttgaacaag aatttggacg cccgctttct tcgatcgagt tccagcggat
     3181 cggacaatgg ctagaagaag accattatca gccggaaatt ttgaagctag cactgcggga
     3241 agccgtatta aatcaggctt atagttttaa ttacgttgac cgcattttgc tatcgtggga
     3301 aagaaaaaat ctacgtacaa aacaacaagt ggaagaagac caaaaacgaa gaaaacagca
     3361 gcttcttcaa agagaagtag aacagtcagc tcaacaagaa gaattgccga aagtctcact
     3421 gaaaaattgg ttggaggagt agcggaatat gttaagcaaa caaaaaacaa tggaagctct
     3481 agaaacgatg tatggaatgt ttcccgaagc acatggagaa ctcaagcaca ataatccatt
     3541 tgagttattg attgcagtga tcttaagtgc ccaggcgaca gatgtatcag tgaataaagc
     3601 aacgccagat ttgtttgcct cttttccgac acccgatgcg ttagcagaag cttcaatcga
     3661 tgaaatcatt cttaaaataa aaacgattgg tttgtatcga aataaagcaa aaaacatcaa
     3721 agcgtgtgca cagcagctga ttgaacgttt cgatgggcaa gtgccaacgt caagagaaga
     3781 attgatgtct cttcctggtg taggcagaaa gacagcaaat gtcgtattgg gggatgcatt
     3841 cgggatacca gcaatcgcag tagataccca cgtggaacgt gtctcaaaac gcctgaggat
     3901 ctgcaaacta gatgctacgg tcatggaagt agaagaaaca ctgatgcgga aagtaccaca
     3961 agaattatgg gtaaagacac atcatacact gatttttttt ggtagatatc attgcacggc
     4021 aagaaatccc aagtgcgagg tatgtccttt gctatcaata tgtcaagatg ggaaaaatcg
     4081 gatgcgtcta aaagaaaagg cgctaaaaaa gaaaccacgc ctgtagacgt ggtttcttta
     4141 attgcaaata aatgtatgaa ttatcttgtt attatggaga aaagctgttt cgtttatctt
     4201 ttttgcatga aaaagtaaat gtacatactc agtagctagg atggtaactt attacaattt
     4261 attgcgataa aaataaaagt tgttattttt attgggaaaa tacccgatgt agaaaataat
     4321 gccttccaat atacctataa aataagtcca gtattccaaa ggattatata aataagtgac
     4381 gtatctatct aaaggtgtaa taaaaatgat aagtgataat aaaaaaatac catttataac
     4441 taaacataaa ctaatggatt tgggcggttt caggctcatc agaaaaatat agattctggt
     4501 gaacaacaac cataaaaaag tgagaatcat tgcatgagag cttctttcca taaattttcc
     4561 tttaagagta gtgcttaaaa gtacagaaaa aataataaat gcaaggatag gagcaattaa
     4621 agtaaattta tttaataata aacaaaattt ttcagctttt ttattcttgg aataacgagg
     4681 tattgttttc gataaaaaaa gatggtgcag acaaattatg ataattaagc atactatcag
     4741 ataactcaaa atgaagcctt ctttcaaaaa attattcaaa accatatatt gtatttttta
     4801 tttgataata acaatgggat tattaagaag tggcactaaa taaaagacta gtgtgtattt
     4861 agccgattta attataaaca agtaaatgga taaagtatat gtaatgataa aagaaaaatg
     4921 gattttctaa tttttaagct aaatgttaga tcacacatgt atatgctgga tagtcttata
     4981 aaaaatcatg taatacagct tggtacatga tttttatttg tagctgtttt ttacatagac
     5041 acaaaaaaga aaccacgctt gtagacgtgg tttctttttt actatctgtt tccagaagct
     5101 gaggtgctgc tggatggtgt atggtcattt gcagcatccg ctccgctagt tgctggctca
     5161 ctggaagaag caggaggagc agatgaagac ggtgtcgagc tttcttcgga tgaagaagaa
     5221 ggaaccgttg ttgaagacgc tggctcgctt gtttctgctg tcgtgctttc ttttgaggac
     5281 tcagcagttg attcgctttg actagtagag ctagagcttt gagtcgttgt ctctgttgtt
     5341 gagcttccag gagtctggac atagctggaa gatggaatag ttgttgatgg tgtaattgca
     5401 ttcgatctag ctgtatattg atctttgtag tacagttcac cgcccacacg aattaatccg
     5461 cttggcatct cccagtctgt atttgtcacg ttagctgaaa catactgcat aagctcgcga
     5521 taaacatcag aggctacgtg tgacgattct gaagtgacag gtgtcatttt tttattgtaa
     5581 cctgtccaca cggaaatcga ataatttggc gtatagcccg caaataggat atctggatac
     5641 accccagaag aaataccaag ttttgcatac tcgtcatctg tgtaattgga tgttccggtc
     5701 ttaccggctt gatataaccc agcgatttgc gcattcgtac cagttccttc agtgatcgta
     5761 tcttttaaaa tgtctgtgat catataagct gtttctggag acatggctgt ttttccatct
     5821 ggctcataag tttcttctgt tccgtcttgg aagacgattt tattaacata ttgaggtttg
     5881 taataagtcc ctccattggc gaaagccgca taggctgctg ccatttttaa ggaagaagct
     5941 ccgtattttg tcccatcttg ttcttccgta ttactagaga tcgcattcga ttgatggatc
     6001 gtactgtatt cgatcccaag attttttaag aaagaagcca ctttatctga accgacttca
     6061 ttgaataatt tgactgctgg aacattacgg gataagtaaa gtgcttggcg taaagtaatg
     6121 gtgcccatat attgattatc ccagtttcct acaggagtcg aagtcccttc atagttatac
     6181 ggtgcgtcag taatagtttt tccagtagaa tatttcaaat attcaaaagc tggtccgtaa
     6241 tctgtgacag gtttcatcgt agaaccaaaa tcacgggaag tattcactgc taagttgttg
     6301 cctaaagtta cgtcttctgc gatgtgacgt ccgccaatct gggcttttac tttcccggta
     6361 ttggtgtcga taagggtgga ggcaacttgc atctcgtcgt ctgggtaaga gacatactga
     6421 tccgtgttga cgatatcata tagtttcttt tgggcgtcca gatccaaatt cgtataaatc
     6481 tctaaaccgt ctgtgtagac attcttatct gttttttctt gtacttcatt gatgacttct
     6541 ttgacataat tatcgacgat cttagtattg tcatcggatt gagttagttc ttgcagacca
     6601 tccgttaccg gtacattaac agcttgatca tattctgttt gagagatttt ttcgttttga
     6661 agcatcgtat acaaaacagt atctcgtctt ttcttcgcct gatctggata aacataagga
     6721 tcataggcgg aaggggcttg aggcatccca gcaagcaatg ctgtttgcgg taaagaaagt
     6781 tctgataatt tcttaccaaa gtacatctca gaagctgtct ccatcccgta caagccgttt
     6841 gacatataca ctttatttac gtagtaggtc aagatctctt gtttcgattt tttttgttcc
     6901 aatcttacag ccatccacgc ttcttgggcc ttgcgtttca gagtctgatc ctcggcactg
     6961 gtggagaaga aggaaagttt gatcaattgc tgtgtcaaag tacttccgcc ttgcagtccg
     7021 ccagaggtaa agttagagag ggcagaaccg atgatccgaa tagggtcaac cccgatgtgt
     7081 ttataaaaac ggcgatcttc taccgacacg atcgcatctt ctaatgtttt cggtaattca
     7141 ttcgcagaaa ttttttcacg cttttctgcg cctaaatctt cgaatagttc gccatcttgt
     7201 gtatatagtt tggaagatac ggtagcgtct aattttttat ccgtcaattc gggcgcatct
     7261 tttgcgtaat accagaaaag tcctacacct gctaaaaaga ggatacagaa aaatgagagt
     7321 attcctaaaa ggatcttgat aaataatcct tttttatgtg ttccagagga ctttccagaa
     7381 cctttgccag aattcttctt cacactcttt ttaggagtag gctgtttctg tcgtctagag
     7441 cttctagttt gttcatttgc cattcttgct ttctccttca atagataatg ttgataaata
     7501 gaagtcaacc gcgtccaaat aaggaatccg cggagaaata ccagttttga tctctattcc
     7561 tagttcttcg atcaaagata aaggtagtga tttgcgtcct ttttcctttt gggcgtccca
     7621 gtaagcaatc aaatgaacac tattcaaata aaaacagcgc ttcaaagacg aaaaccataa
     7681 caagacaaag cagaccccat tttgttcgag gcatgctttc atatgttcga tttgatgggc
     7741 atggaagttt ttaaagggaa aagatgtttt gttcttggtt tcttttgctt caaagtccaa
     7801 gtaaaagcct cgatagacgc cattatagtc tgtagttgag gcttgggtaa agtaggcttc
     7861 cttgatcaca gcagcacttc tcttaggata gtctactttc acgatttgga taggggtagg
     7921 ctttttatgg ataaccgcca acccgcggga aagataatat ttattacttt cattgattgc
     7981 ttcttcaaag cgcatccccc gattgccgaa ttcagtgatc tgatttcttt tttcttgaat
     8041 tttttcgacg ggagaggcat tcttataata aggttggcca tttggatagc gcaataccat
     8101 ctttaatcac actcctaggc cacattatag caaaaaagcg gatagaaaga aaaggaatct
     8161 gagatggata agtcaaaagt aatctatgtg tctgggtatc gaagtttcga actcggtgtt
     8221 tttaaagaaa atgatcctaa aatccaagtt gttaaaaatg ttttaaaaaa agaactccat
     8281 cagttagcag aagaagggct ggaatgggta cttgtcagtg ggaatttggg cgtcgaacta
     8341 tgggctgtgc aaatagtagc ggaattgaaa gaagaatatc cggaactatc tcttggtatt
     8401 ttgtatcctt tttctgattt tggtaataac tggaatgaag ggaaccaaga aaaaaaacaa
     8461 ttagcagaac aattagcaga ttatgtcgaa gctgttagcc atcaacccta ccaatcacct
     8521 agtcagctaa agaatcacac tcgttttata cttgagcata cagtgggctg tctgctgatc
     8581 tatgatgaag aattccctgg aaaaacgaag tatttttttg aagatgcaca gaattaccaa
     8641 gcagatcatc cctacgaaat tcggctgatc agtatggacg atcttcaaaa ttttagtgaa
     8701 taatcgatat tgtgtttgat ttatcaactt ttttcctata caatgaaaaa ggtagaaatg
     8761 ttattacgta aatgaaaact agaggtgtaa atatggcgaa tttggtttat agtcctaaag
     8821 acattttaca acaagaattc aaaacaaaaa tgcgcggcta tgatcccgtt gaagttgatg
     8881 aattcttaga caatatcatc aaagattatg aaacttacag caaagaactc cttgctttac
     8941 aagaagaaaa cgatcgattg agtgcaaaag tagctcaatt atctaaaact caaggagcag
     9001 ctcaaacacg cgtgcaacaa acagaggcac caaagagcgc agcagtgacc aattttgata
     9061 tcttaaaacg cttgtcaaat cttgaacgtg aagtatttgg taaaaagctt gatcaacaag
     9121 caagtgcagt caaaccagca caacctaatc caaataacta cacaaatgca gacacaagct
     9181 tagatgataa tgaaaaaaca cgtcaatttt aatttttgat taatgaattt gcggttttcg
     9241 ggtaatcgcg gtctgacttg atcaggctga ggaaagtcca tgctcgcaca ggctgtgatg
     9301 cctgtagtgt tcgtgcttag cgaaaccata agctaaggta ctctttggag taacggcagg
     9361 aaaacaagct aaggcttcgg ctatgcttaa gtatccttga aagtgccaca gtgacgaagc
     9421 ggctggggaa acccatccgg tggaacgcgg taaacccctc gagcgagcaa cccaaacaat
     9481 ggtaggggcg ctttttgtct ggaagaagaa caggacaaaa aggcagtata actgcagata
     9541 gatggttacc gctttgtttt ttctccctgg aaaagcaaag tacagaacat ggcttataga
     9601 aaaccgcaaa catcagggaa ttaggtcgtg acaaaagtaa tgcgttataa gaaacaagaa
     9661 aaacatttga aagctatttt tcaatacttc agatgaatcg ttcttattac aaaacaaact
     9721 tttgtatcac cggttattcg gaatttgagt aaaagtgtgg ggtaggactg atgtcccacc
     9781 tccttttttt ataaataagg agattgtgta tggaaaaaaa caggaagttc aacctcgtcg
     9841 caactgcggc aagcgggcta gaagcactgg ttggtaaaga attaagagat cttggaatcg
     9901 aatgccaagt tgagaacgga cgtgcacgtt ttgaaggaac aatggaaacg atcgctacag
     9961 ctaatttatg gttacgtaca gcagatcgag taaaaatcgt ggttggggaa ttcgatgcgt
    10021 atacgtttga tgaattgttt gaaaacgtaa aagcattgcc ttgggaagat tttcttccac
    10081 tagatgcagc ttttcctgta gctggaaaat cgatcaagtc gaaactatat agcacacctg
    10141 attgccaagc gatcacaaaa aaagcaatcg ttgaacgtct ccgtacctat tatcaccgtc
    10201 cagcttctgt accactgacg gaaactggcg cacattttca gttggaagta gctttgctga
    10261 aagaccatgt gatggtaact ctggatacga caggaccaag tttattcaaa cgtggttatc
    10321 gcttagaaaa aggtggagca ccattgaaag aaaacatggc agcagcactt attatgttga
    10381 caaattggcg aaaagaccgt ccattttatg atcctgtatg tggttcaggt acgctttgta
    10441 ttgaagcagc cttaataggc cataatatcg caccaggatt caatcgtgag tttgcttgcg
    10501 aatcttggga ttggttcagt caagaaatca tggaaaatgt tcgatcagca gccgaagaaa
    10561 aagcagatta tgatattgaa ttagatatta ctggttcaga tatcaatgga cggatgattg
    10621 aaattgccaa agccaatgca gaagaaatcg gattaggctc ttcgattacc tttaagcaac
    10681 aagcagtcaa agacttcaaa acggataaag aatacggggt catcgtggct aatccgcctt
    10741 acggagaacg tctaggagaa gaagaaactg ttcggaaatt atataaagaa atgggcgatg
    10801 tattccgtcc attaaaaaca tggagtaaat atatcttgac tagcgactta gcgtttgagg
    10861 aattttacgg acaaaaagca accaaaaaaa gaaaattgta caatggtgca ttgcgtacgg
    10921 atctattcca gtattgggga actcgtccgc ctagaaaaaa agagaaagac gaataatctt
    10981 actaattgat tcattcaatt ggatgtttgg aggaataggg aatgaaagaa aaagagtttt
    11041 tacaagaagt aaaagaaatc aatcttctac aacaagcttt aggaatcctc gattgggata
    11101 cacaaacagg tatgcctgaa aaagctagtg cataccgaag tgaagtagac agctatcttt
    11161 acagcattta tttttcaaaa aaaatcggtc caaaaatcaa agaagccatt cgctattttt
    11221 ctgaacatcc ggaagaattg tcggaagttg gaactgccgt ttttgccaaa gtaaaagaag
    11281 agtatgattt ggaacatcgc gtaccggaag atcttatgag aaaatataca gctgccgttt
    11341 cttcagcaca cggacaatgg caaaaagcta gagaaacaca acaattttct gattttcaac
    11401 acgcattgaa tcaaaatatt gaattaacaa aacagttgat accttactgg agaaaagatg
    11461 aaaagacgaa ttatgatgtt ttgctgaatc aatacgaacc cgggatgact gtcgaaatcc
    11521 ttgataaggt atttgatcag cttaaagaag gaattctttc gatccgccgt acgctccaag
    11581 aaaaagggac tgaacctgaa acagactttt taaatcggag aatgacgaaa aaacaacaga
    11641 agagatttgt agtgaaagta atcgaacaac ttggttatga ttttagtcgt ggtcgtttag
    11701 atgacactgt ccacccattt atgactggaa ttaatccgaa tgatgcacga atcacgacac
    11761 gatggaacga aacagatttc aaaatggctg tttttggag
//
LOCUS       DACEXQ010000072        11564 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.072, whole genome shotgun sequence.
ACCESSION   DACEXQ010000072 DACEXQ010000000
VERSION     DACEXQ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.072"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            39..308
                     /gene="rpsN"
                     /locus_tag="IXH04_001363"
     CDS             39..308
                     /gene="rpsN"
                     /locus_tag="IXH04_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAQ1851980.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            323..502
                     /gene="rpmF"
                     /locus_tag="IXH04_001364"
     CDS             323..502
                     /gene="rpmF"
                     /locus_tag="IXH04_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAQ1851981.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            534..911
                     /locus_tag="IXH04_001365"
     CDS             534..911
                     /locus_tag="IXH04_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851982.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            932..1081
                     /gene="rpmG"
                     /locus_tag="IXH04_001366"
     CDS             932..1081
                     /gene="rpmG"
                     /locus_tag="IXH04_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ1851983.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1105..2088
                     /locus_tag="IXH04_001367"
     CDS             1105..2088
                     /locus_tag="IXH04_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ1851984.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2118..2240
                     /locus_tag="IXH04_001368"
     CDS             2118..2240
                     /locus_tag="IXH04_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAQ1851985.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2258..3781
                     /locus_tag="IXH04_001369"
     CDS             2258..3781
                     /locus_tag="IXH04_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAQ1851986.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIIGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNEMHNWPTFYPASLSKHEIAQEMMAH"
     gene            3805..4044
                     /locus_tag="IXH04_001370"
     CDS             3805..4044
                     /locus_tag="IXH04_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1851987.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4237..4698)
                     /locus_tag="IXH04_001371"
     CDS             complement(4237..4698)
                     /locus_tag="IXH04_001371"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1851988.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(4893..5111)
                     /locus_tag="IXH04_001372"
     CDS             complement(4893..5111)
                     /locus_tag="IXH04_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1851989.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5229..6845
                     /locus_tag="IXH04_001373"
     CDS             5229..6845
                     /locus_tag="IXH04_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ1851990.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(7086..8426)
                     /locus_tag="IXH04_001374"
                     /pseudo
     CDS             complement(7086..8426)
                     /locus_tag="IXH04_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(8702..8857)
                     /locus_tag="IXH04_001375"
     CDS             complement(8702..8857)
                     /locus_tag="IXH04_001375"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAQ1851991.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(9049..9243)
                     /locus_tag="IXH04_001376"
     CDS             complement(9049..9243)
                     /locus_tag="IXH04_001376"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ1851992.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            complement(9153..9485)
                     /locus_tag="IXH04_001377"
     CDS             complement(9153..9485)
                     /locus_tag="IXH04_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1851993.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRLAISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(9999..10190)
                     /locus_tag="IXH04_001378"
     CDS             complement(9999..10190)
                     /locus_tag="IXH04_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1851994.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            complement(10284..10670)
                     /locus_tag="IXH04_001379"
     CDS             complement(10284..10670)
                     /locus_tag="IXH04_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ1851995.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            11364..>11564
                     /locus_tag="IXH04_001380"
     CDS             11364..>11564
                     /locus_tag="IXH04_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAQ1851996.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFNMIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFN"
BASE COUNT         3862 a         1803 c         2221 g         3678 t
ORIGIN      
        1 gaggtgtcta aatcaaaata aaaggagtat aaaggtatat ggcaaaaaag tcgaaaattg
       61 ctaaagcaaa gaaacaaatg gcgatgattg aaaagtatgc agataaacgt caggaattaa
      121 aagccgcagg agatcgcacc gctctcgcta aattgccaag agattcaaat ccgaatcgtc
      181 tgaggcttcg tgatcagacg gatggtcgtc caagagggta tatgcgaaag ttcggtatgt
      241 cccgtattaa gtttagagaa cttgcccatc aagggttgat tcctggagta aaaaaagcaa
      301 gttggtaaaa ggaggaaata tcatggcagt accagcaaga aaaacatcta aggcgaagaa
      361 acggttaaga agaactcatc aaactgtagt aaaaccagaa atttcatttg acgaagcgaa
      421 tggtgattat agacgtagcc atcatgtatc tttaaaaggg tactataatg gaaagaaagt
      481 aattaaagga gcttcaaaat agtttaatat ataactttta ggagggaatc aaaatggaaa
      541 ttatttaccc acctttagtt gaagaaggat taaagtatta tcttgaaacg acccagcaat
      601 cgctagataa atgcactttt tatcgttcga tggtagagag aggaattatt actgaaacgg
      661 gtttaccgac ccaacaggca attgaaaatg gattagtcaa agattactat gaggaccaag
      721 gcttatcatt tgatgatttt ttaagaatct atccaatttt tgaagagtat gacgaggagt
      781 tatttcaatg tatcgatggt tattgggaga tacctgttga tatgaaagaa aatttagttt
      841 cacaattgga atctggcgag ttaacttttg aagatgcaca acaaattcaa gcatatcttg
      901 aagatcgata agtattgaag gaggaagatt tatgcgtcaa aatattattt tggaatgtgt
      961 cgaaactgga gaacgtctgt atctcaccag caaaaataaa cggaataacc ctgaacggtt
     1021 agaattaaaa aagtattcgc caaaattgcg cagacgggca atttttaaag aagtgaaata
     1081 gctaagtggt aaaaaggtga caaaatgggc ataccagtta caatcgtttc tgggttttta
     1141 ggggctggaa aaacaacgct aatcaatcaa gccttgcaag tctcacccta ccccagggaa
     1201 gaaattatta taattgaaaa tgagtttggt gaagttggga ttgatcacaa attattagtt
     1261 catagcgagg aacgggtgtt acaactaaat aatggatgta tgtgttgcag tcttcgcagt
     1321 gatttattag caatattgat ttctttactg gaaacaatag aagaacatca acaaccaatt
     1381 tcacaaatca tcattgaaac tacaggtata gcagatcccc aaccaatcat tcaaacactt
     1441 ttaacagcgc ctcaactaag aggaagagta tatatcgata gtttattgac tgttattgat
     1501 agtgacagtt ttgaacttat tgaaagagaa ccacaagccc ttaagcaact tgtcatggca
     1561 gatcgcattt ttgtgtctag aaaacaaaac ttgaatttat ctgcactaaa aaaattacag
     1621 agagaaatca aaagtattaa ccctttatcc gatattcgaa tgttcacaca tagtgaaacg
     1681 attcaaaaaa aggattttta taatttgaaa aaatttaatc atcccttagt ttttgaaagt
     1741 gattcatcag aaaatcacta taatggcaaa tactatcatc aacaccatca agtacatgag
     1801 tttaaatcaa ttctcttaac ttcagatcaa gacataaaaa aagatttttt actccaatgg
     1861 attgactggc tgattttaac caaccaaggg agcctgtatc gttttaaagg attgattgct
     1921 ttacaagagc aggattttat agttgccatg caagaggtaa acgaacaggt taattttcaa
     1981 tacaccactc gacaaaaaga ccaaaccact attattctaa tcggaaaaat gttggatgaa
     2041 gaaaggatca aacagagttt tgataaatta ctggcggaaa gcaaataatg ataagtagtg
     2101 attgggggta atttctaatg gagaaaacgg atatatctag tgcatatcga cgacttaaaa
     2161 gcccgaatat taaaactaga aaaagggcat taaaaattat caaagaacat aaacgaaata
     2221 aaatgaagaa attagcataa ttaaggaagg aaaaagaatg aagaaaatca gtgtaggact
     2281 tattgggata gcagctctag gattattggg agcttgctcc agcactaatg atgcaaaagt
     2341 atcgaatgat aaagatggaa aattagaaat tgtaacaaca ttctatccaa tgtatgattt
     2401 caccaaaaac atcatcggcg atgaagcgaa cgtggattta atggttccag cgggatcaga
     2461 acctcatgac tatgagccct cagctaagga tatggcaaaa gctcatgatg ctgatgtttt
     2521 tgtatatcat aacgaaaaca tggaatcatg ggtaccaaaa gctaaagaaa gttggaagaa
     2581 agctgggcct aatgtagtag aaggaacaaa agacatgatt ttattgccag gtagtgaaga
     2641 agaagatcat gatcacggag aagaagatca ccatcatgaa ctagatcctc atacgtgggt
     2701 ttcacccaaa atggcaatta aggaagtttc aaatatcaag gatcagttag ttaaattgta
     2761 tccgaaaaaa gcaaaagtat ttgagacaaa tgctgaaaag tatttgacta aactgaaacg
     2821 gttggatgct gactatacca ctagtcttaa agaagcgaaa caaaaaagtt ttgtgacgca
     2881 acacgcagcc tttggatatt tagctttgga ttatggattg aaacaagttc ctattgctgg
     2941 actaagtcct gaagaagagc catcttctgg tcgattagct gaattaaaag agtatgttaa
     3001 aaaaaataag atcaattata tttactttga aaagaatgct aatgataaaa ttgcaaaaac
     3061 tttggccaac gaagctggga taaaattgga agtactgaat cctcttgaaa gtcttacaaa
     3121 agaacaaatg gataatggtg aagattatgt ttcagtaatg gaagacaatc taaaagcgtt
     3181 agaaaaaaca actatggttg ctggtgaaga ggtagttcca gaaaaagaag cgaaggatga
     3241 aaagacagtt gctaatgggt actttaaaga tgccgatgtg aaggatcgcg aattgagtga
     3301 ttatacaggc gaatggcaat ctgtttatcc gttgttaaaa gatggaattt tggatgaagt
     3361 tttcgattac aaagcaaaaa tcaataaaga catgacagct gccgagtaca aagattatta
     3421 tactactggc tataagacag atattgatac tatcaacatt aaagacaaca ccattgattt
     3481 tgtagtcaac ggtgaacatc atcaatacac atacaaatat gttggataca aaattttgaa
     3541 ttatgaaaaa ggaaaccgtg gtgttcgttt taattttgaa actgatgatg caggtgctgg
     3601 tcgttttaaa tatattcaat ttagtgatca tggaattgca ccaagtaagg cagagcattt
     3661 ccatattttc tttggtggag aaagtcaaga aaaactctat aatgaaatgc ataattggcc
     3721 tactttctat ccagcttcat tgtctaaaca tgaaattgct caagaaatga tggctcatta
     3781 aaatccaatt tttggaggaa atacatggta agaaaagtat tattgtctat ggattccaag
     3841 aatattgatg cttatcggcg ttatcgattt gcaaagcaaa tgagattaaa tgggagtaca
     3901 ttagctgata ttgctgcaac tttacatgtt agtattgaca cagttcatag agtattaaaa
     3961 cgggatcctg attgttgttt tatcaaggtc aatactggaa gaagtagacg tttagcaaaa
     4021 cggcataata gcttggccaa ttaattgata ataccagagt agatcatttt tcccttattt
     4081 ggatttgatc tgctttttat tatgtcttga attgtcaaat caagcgcaac actttatcaa
     4141 agaagacttc ataaggtgtt ctccaatcta aacactttct aggtctgtta ttcagttttt
     4201 caatcatctg atttatcgtt gagttatcta ccgtagctat ctctttcgat tttggcaagt
     4261 attctcgaat gagtctattc gtattttaat ttgttcattg ttgccaaggg catgaggatc
     4321 tggaaaataa aagggcatat tattcaatgc ttctgtaact gcgaaatgat tggagaattc
     4381 ttttcctcta tctggagtga tcgttttgac cgcctcattt ggaatactgc ttaaaagttc
     4441 aatcattttc tcgctcacat aagcggcttt ctttctgggg accttctttc ctgctaaaag
     4501 gaagcatgat tttctgtctg tcaaagtcac taaacaagta ccaccagttt tgccagctac
     4561 aatatcggct ttccaatgac caaaatcact tctatcatct gcggattttg gacggccatg
     4621 gatcgaatga gtaaggcaaa tctttccacg agactcgaca tgattttttg tatgtcttgt
     4681 ttttcctcga tggtgcaatg aacggatcag gcctttattg ccgtgtgaga ggggttctgt
     4741 atcgaaaaga tcattgcaaa ttgcacgtta gatcgtgata aaactaattg aaaagtccat
     4801 atgttcatgc ttcaaacgat tcgagatttc ctctggcgac cactgaaaat cgacaaataa
     4861 atgacgaaca attgaccaaa ctcacggatc atttagaatg tgataacgtc cgcatttttt
     4921 tcttcgataa gcataatttt tttccgcttt ggaaggtgaa tagttttttt cttgtttctt
     4981 ctcagttcat gtaaaatagt aaacgggcta tgctgaatgt cgttgccaat tatactaata
     5041 gaaaatcctt cttcgtgaag cagaaagatt ctttctcgtt cgagtatagt aagatgacga
     5101 tactggctca taattatttt ccttctagga gtgttctcga caaacttata ctagttggat
     5161 atcgattatg actcattttt atgtgttgca cttaaagtgt aaattcaagg tatagaaaga
     5221 ggttccctat gaaaaaaata cctattacaa tcttaactgg ctatcttggt tcaggaaaaa
     5281 cgatacttct aaatcatatt ctgtacggtg atcacggaaa ggaaattgct gtcattgtaa
     5341 atgacatagg ggaagtaaat gttgatgggg tattagtcga caagaaaggt tttaaaagaa
     5401 ctggagaaaa acttgtttcg atgccgaatg gatgcatttg ttgtacatta cgcgaagatt
     5461 tgattgttga actagatcaa ttagtgcaat tttctgactt gaatcaaatt gttattgaag
     5521 cttcaggaat cagcaaacca attccaatta ttcaagcaat tatattagct aaaaaccctt
     5581 tagacattga tttgacccaa cgattagaga ttggtgccat tattatagta gtggatgtaa
     5641 aacgtttttg ggataatttc gaaacgggtg aaaagattca ggaaagaaaa gctgaaggta
     5701 atgtagttga agaagaacag gatattcgtg gcttgttaat tgatcaattc gagtgttgta
     5761 acatattact tttaaataat agtgacttag tgtcaaccaa gacgatttac aaaattaacg
     5821 gtctaattgc taagttacag tccaatgcca aaataattag gacaattaaa ggtgaagtag
     5881 acttatcgaa aattctaaat acaaatcttt ttaatataga tgagacttcc tattcagtgg
     5941 gctggttcaa agagttagag cttggctatc aaaatcatat tcctgaaaca gaagaatatg
     6001 gtatctcaag ttttatctat cgaaatcggt tactccttga cgcacaaaaa tttgcccatc
     6061 ttacggataa aaattttcct ggcaatatta caagagcaaa agggtttctt tggtttgccc
     6121 aacatagtga tttttcgacg atctttgaaa catcgggtag ctataaaagt attgaatcat
     6181 taaattattg ggtagctagt ttacctaaaa ttgagtgtca aatgattata caaaaaaatc
     6241 ccgaattgaa ggaagcctgg catccgttgt ttggtgaacg cattaataaa ctagttttta
     6301 ttggtatgaa gatggatcaa caatgtatta aggaacaact tgagggttgt ttaactgatt
     6361 ctcttaatgc agataatttc cgaaaaattt caccatttcc atggaaaaag tctaatatgt
     6421 ttagccagat atcgttaatg aaatttttaa gaaaagctgg tgtcaagata ggcgatgtca
     6481 taattattgg cattttgatt attacctctt ttattccctt atttgtattc tctatagaaa
     6541 aggagaatga aacaactgaa gatgctatca aatatgccat cgttcgaatt gatggcaagg
     6601 aaatggatcg cttcgatttg gataagatca atcataaact cgtgaattat catcctgcga
     6661 agggacaata taatattgtt gagattaaag atggaagaat tcgagttaaa gaagataata
     6721 gtcctgatca aatcgcggtt agaactggat ggatctcaaa acccggtcaa acgagtattt
     6781 gtttacccca taagttagtt attagtattg agaaaaaaga aagcacagat tactacattt
     6841 actaatctgg aaatagatga ggaggttttt aataagaaaa agatattatt attaataata
     6901 gcaggggctt tattaagtgt gttaagcgct tgtggacaag aaaaagaagc ggtgacaaca
     6961 ggcacaagta tctttaacgg gacctgcaat ggtttagtta acagtcggtt agattactta
     7021 ttttggctct tcgtcaatga gtgttggtga agaaattcaa tagaataatc gagatactca
     7081 cacggctaga cagctatctt gtctagccgt gttgttgttc tggctttagt cagttgggta
     7141 gtattcttag ttttcttaaa atattttcct cgatataaaa agattcgttc tcggtagttt
     7201 tgaaagtttc taaagccata ggcaattcgt tttagtactt tgatatggtt attcaaacat
     7261 tctatagggc cgttggaata gggaacgcgc aacgctcgtt tgatttctgt ttggtatttc
     7321 ttgaaggtcg tgaacactgg ttggtagtgc tctggcaact ccttgtagtc ttcttttagg
     7381 agcgcgacga attttggaac agcttgtctt ctaatagcgg aaagaaaatc ctggtagact
     7441 tcgtagcctc gctttaagga gtcatcataa gataatagcc gatcgactat ctccgcttct
     7501 gtcagatgat ctcgaaatga cggacgccag tagcgatgct ggcccttcaa ttcccacgca
     7561 ttcttttgaa gtagtctcca gtatttcttt aagtgcttcg accgacgatc ccttttttta
     7621 aaggtcttca ttacttgaat tcggtgattg gtaaaggccc gatcgatgtg ttggctaata
     7681 tgaaagcgat cggcgacgat tcgggcattc ggaaaacgct ctttcaccaa tgtgcgatag
     7741 ggtgtataga aatctgaaac cactagacgt acttgttcac ggacttttct agggtagcgc
     7801 aagaaatagc cgcgtaaagg attgagttgc cgattttcca caatatcaat cagttgatag
     7861 gtcttgccgt ccatcatgac aaagctcatg gctcccgaga catttttcac tgatttgaat
     7921 tcatcgaaac acaccacttc tggtaacagc tggttcaaag aaatggtttt aggccgaagg
     7981 ctgcgaagta ttcgaatgac agtggtcgga gaaatgtgct tcatacgtgc gatcaaagac
     8041 atagaaagag gttcgcttaa tagctcaaga atggcttgtt tgactctgcg agcaatggag
     8101 cagcgacgat cactaactga atcttctgct aaaaaagtct ttccacagtg tttacatttg
     8161 aagcgctgtt tgttaatccg cagaatggtc ttgtagttac tgacatcatt caataagatg
     8221 gatacttttt tccagcccta tttaatgatc gtttgttcat tttgatagtg gcgatgaaag
     8281 cattcctccg gttgataggt caatgtgcca tcaaaagcaa aacatgtttc gtcattgatg
     8341 cgctctttgc gaaaggcatc ttcatgaaag gtaagattta ggtctagaat atctagtaac
     8401 tctttggtaa actctgaata ggacatcttg aaacactact ttgaatggtt tttgtcgact
     8461 ttattctaaa ggattttaag gtgtctgttt gcgtttatta gaaaaaagaa aaagtattgg
     8521 caacagaagg cgtcaccaac actagatatt atagatccct tattttcttc gcttcaaatt
     8581 gtcaaggagt catcataaat tttgtatgga ttcttttgtt tgggtagtag tcttctaaat
     8641 aattttggat taacaccttc atctctattt tacttttata tttgttggag tcgattaatt
     8701 ctcatttgat tgatcgatga aacgcttcaa ttactgcatt gtcaacagga gtcccagttt
     8761 ggctcactga atgtgtgatg tggtggtcgt tcaacagctc ttgataaata aaagaccgat
     8821 gctggctacc tctatcggaa tgaattaccg taggcaattc tccttaataa atttgtaatg
     8881 gacggacgac aatctctttt ttcatgtgtg aatctaccat atagtaacgt agaatacgag
     8941 ttgctaagtc aatataggtg gacaaataga ggcgaccgtc attacatgga atgtaagtaa
     9001 tatccatcac ccaaacagca tcaatttggt tctgttcaaa ttatcgattc aacatttttt
     9061 taccatttct ttgattgttt tttctctacc atgagaagat ttgcgtcgat gaaatcctct
     9121 tgtgcataaa ttcatttcac gcataagaac agctacaaca cgtttattga tttcgattcc
     9181 ttcatcgtaa agtttttgag tgatccctgg agatccgtat cgatctttgt gttcgtagaa
     9241 cactcgtctt cctcttttag tcgtttattt tcctgttcaa gaatgtagaa atctgcttta
     9301 tcaggtaatc tttttccttg tccagcaaaa gcatttttcc cgtatcgacg atattctgaa
     9361 acccatcggg aaattgctag acgagagata ttgtattctt cagcaatttt cttggtagtt
     9421 acttttttct caaggatatc ctgtgaaatc tttagtttaa atttacgatc atatgttttt
     9481 ctcaatagag ttcattcctt ccggtaacgt aactgatagt caagaaaagt gtatcatagc
     9541 ccaacttagt atcagggttt gtcaactaaa ctgtggaagt tgatagataa gagtttttaa
     9601 caaccaaaat tctcttgact atttttgaaa tcagacaaat tttgattgga cacatagttg
     9661 aataaatcta ccgtcgttac ggaaggtaaa tcgcatgctt ttcaattcta gaggagaagt
     9721 cctcgttgaa gtaccatagc ccagttctgg attcgacctc ctgcccactt catacgtaaa
     9781 taaagtaatt ttaggagtga gactgtgaca aaagttattt ttaagctaaa aacgaattaa
     9841 aaaacgttga taattaaaat cattcgtttt agattctatg aatcattata gaacgctgta
     9901 aaatcgatac tttataatta taaagaagat agtttcaaat ttattgttcg aaatttgaaa
     9961 ctatcttttt acttatgtca cagactccct tagttgagtt attctttaat ttcgaatttc
    10021 gagatagcta gatatattgc aagcatacat tcttgataac aatcttgatc atattttcca
    10081 gttctccagg attgcttatg aatcaaagag ctaaatcttt tgagaattgc ctcaacagct
    10141 tcttcatttc cagcctttgc taaaatgaaa tcttctttta aagaattcat aaatctacct
    10201 tcttcccatg gacttataat ctatttcttt gggaaggagg agaacgagca aagaagcata
    10261 caaaattagt tattatgcag atattaaacc agcatttttt gaattttttt gtaaagtcga
    10321 tgttttaaat ttgtcgcacc ctgtcttgta atccccagaa cctttccaat ttctttatca
    10381 gttttatcaa agataaattt ttcgataaga aaaatttgtt caggttcttt caacaaagga
    10441 aggatttttg ctaattttat atcttcaaca aggagagtca catttaaaac attaatcggt
    10501 tgtttaaatt ttatagtatc tttagtaaac aaatatggtg ggagttgatc aagttgttca
    10561 tgtttttgaa tttcttcttg ttttttataa taagtattag cggtgttcaa aatgaccttt
    10621 tcaaagaatc gaatcaactg ggcatttacg cgttcgtcgt tatctgacat attgcacacc
    10681 tcctccatta agatttaaag ggaaaagagg caaaaaacac gaaaagaagc taatgcaatt
    10741 atgctgcgct gcgaataact tctataagct cacatgcatt gataggactg ctgatgttta
    10801 ttttcaaaaa tgttttttta gttgataatg gatagttatc tttatactta gaaaagaaaa
    10861 ttcatatatc ttcttaaata tcaaacatgt ccttatggct aagaaaatgt cttgagcttt
    10921 ttacgtataa taaatctttt ctaaaacctc cttttggata atattcttat tcgtgtatag
    10981 attgtatttt ttatttcttc ccccttcata attaacaact agaaaagagt tcgaatagac
    11041 acgtaattga acgaaaattt ttattggatt cccaaattgg aatatttaga aacgaaaaat
    11101 tcctattatc tgtatagtgc ttagataata acggatcata ataattattg tctttgaact
    11161 agatatatgt aaatagtatt catcatgatg aaaaatgatg agttctcggt gataaaatgt
    11221 actttttatg gtggaatata catgaaagcg ataagtggat attttataat gaagtaagtt
    11281 agcttatttt gacagaggaa tagtatttta gataacgtat aaaatactat ttaatgatgt
    11341 agaaaggaaa gagagaaaca aaagtgtttt cagataaaga agtcttatta aagtctcttc
    11401 atgtttgttt aaagttacct atcgtggttt ttaattccga attcaatatg ataaaagagt
    11461 atcgctcaga tagaactact tatttttttt atgattataa aaagttacta actgataata
    11521 ttaaaaaaac taaagatttc catttcttta ctggtgattt taat
//
LOCUS       DACEXQ010000073        11497 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.073, whole genome shotgun sequence.
ACCESSION   DACEXQ010000073 DACEXQ010000000
VERSION     DACEXQ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.073"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..805
                     /locus_tag="IXH04_001381"
     CDS             <1..805
                     /locus_tag="IXH04_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1851997.1"
                     /translation="KKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATGRP
                     FRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLNLD
                     FIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAGAV
                     SAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGDEH
                     NDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
     gene            complement(991..3144)
                     /locus_tag="IXH04_001382"
     CDS             complement(991..3144)
                     /locus_tag="IXH04_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAQ1851998.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(3539..4387)
                     /locus_tag="IXH04_001383"
     CDS             complement(3539..4387)
                     /locus_tag="IXH04_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1851999.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(4611..6551)
                     /gene="thrS"
                     /locus_tag="IXH04_001384"
     CDS             complement(4611..6551)
                     /gene="thrS"
                     /locus_tag="IXH04_001384"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAQ1852000.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(6888..7067)
                     /locus_tag="IXH04_001385"
     CDS             complement(6888..7067)
                     /locus_tag="IXH04_001385"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAQ1852001.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(7114..8937)
                     /gene="cadA"
                     /locus_tag="IXH04_001386"
     CDS             complement(7114..8937)
                     /gene="cadA"
                     /locus_tag="IXH04_001386"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ1852002.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            9215..11494
                     /locus_tag="IXH04_001387"
     CDS             9215..11494
                     /locus_tag="IXH04_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1852003.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
BASE COUNT         3330 a         2433 c         2060 g         3674 t
ORIGIN      
        1 taaaaaatta attgccatcg atctggacgg aactaccctc aatcaagatt cgatgattac
       61 atctaaaaca aaagagactc taaaaaaagc aatcaatgcc ggtcatcatg tcagtattgc
      121 tacaggacgc ccattccgaa tgagccatca gttttaccaa caattagaat taacaacacc
      181 gatggttaat tttaacggcg cattagtaca catccctaat cagcactggg atggtgaaag
      241 agaaacgttg atcaatcgtg aaatcgcttt tgaaatctta tcgcaaaaaa agcagctaaa
      301 tttagatttt atcgctgctg aaaatcgtga tacttttttt attgacagtt ttgatttctt
      361 tgatgaaaaa atttttgctt cttctcgtcc aggagagaag aatttgcttt ctcctaaaaa
      421 tttgacgaca aatcctacat ctttgcttgt ccgaacagac aaacgttttg caggagctgt
      481 atctgctgaa ttaacacgtc aatttggcag ttatgtcgat gttcggacat ggggtggacc
      541 tactgccatc ttagagattg tttcaaaagg aatccaaaaa gcaaaaggcg tgcaagagat
      601 tgctaactac ctgtctatcg atcaaaaaga cgtcattgcc tttggtgacg agcataatga
      661 tttggaattg ttggattatg caggttgggg agtagctatg gctaacggta ctgaccaact
      721 caaaggcatt gcaaatgatg tcactccttt gagcaatcag gaagacggtt tagctgttta
      781 tctggaaaaa ctacttaaac tataatagag aattagattc cacaaaaaag aggttgtgac
      841 aatttttgtc acaacctttt ttgccgagta aacggtgttc aaaagccagt ttctcggtat
      901 gaagtcaaat ttaagcaaaa atgtgagaag ctattttcac aaaatttttc ttcatactcg
      961 aaactaagcg gctttttcac gacctctttt ttattttgcc atgaacatat cataatacgc
     1021 gtccattatt tctttagcaa tcgtcaagtt tatgtggtct tgttcgtcta ataagttcgg
     1081 caagacaaca ctgatggcaa tttccggatt gtctgttggt ccgtatgcca cgatattact
     1141 attgactgtc gcaatatctg gatgaccttc cgcaaccgtt tctgcagtcc ctgtttttgc
     1201 ggaaaggtcc attttcgcac tggctaatgg tttggctgtt gtgtaaggat cggtaccatg
     1261 gacaacttgg tggaatcctt cacggaggat cgccatattt tctgcagaaa tattgaccgt
     1321 attttctact gttgtgccaa tttctttctt cacttcaccc aagtttccat tttcgtcatt
     1381 gccatagatc gctttgacta ggtgcggctg tactcgtttc ccgccattag cgactgttga
     1441 agcatattga gctaattgga ttggtgtata tgtgtcaaac tgtccaaatg ccaaatcggt
     1501 aaagttacct ggagcatacc attctttgcc attatccgca ttgaacttct tcatataatc
     1561 aacagaacgt gagatcccag gagattcatt cggcaaatcg atcccagtcg ttgtaccaag
     1621 accaaactct ttgaaagagt ctctcaattg ctgataagct ttagcttctt catctaaact
     1681 aggtagtccc attccaggtg tatagtctag tcctaacatc tttaacgcca ctttgatcat
     1741 atacgtatta gaagacaatt ccgatgcttt gactgcatca agagcaactt gtccgcttcg
     1801 attaaagaca gagctttttt ctgaagcacc ttgcagacga atcggctcat caatcaaaac
     1861 ttgatttccg ctgatcgcgt tacttgccca gcctgctgtc aaagtaccag ccttgactac
     1921 agaccctgga tcaaaggcgc tagtaatcgt gcctaaagca ttctcggtga tttctttgga
     1981 accttgttca tgactgaaac cagtcattgc tagcacttct cctgtctgag gattcatagc
     2041 cactgcataa gcaccaggcg aatactgtgc tttgccgttg ttgatcaatg tttgataatt
     2101 ccttttcaaa atttcttcta cttttgattg gaattctgca ttcatagata acattaaatt
     2161 agaacctttt tccccagaga agatttcttt ttgattcgaa acatttcctt cattatcaag
     2221 ggaaacttca tattgggttt tggttccttg caggacatct tcatattgtt tttccaaata
     2281 actttgtcct acacggtcat tccgagaata accttttgct aggtatttct ctgcttcatc
     2341 tgctggaagt ccttgctttt cagtcgtcac actgccaaga atacttttca gtgaacttgc
     2401 tgcattatat tctcgtgtcc agtctgtgcc agtcgaaact cctggaagtt cagaagcgcg
     2461 ttctgccaca acagctaact cttgatcagt aacaccagag tttttgatat agacagtgtt
     2521 taattcatat gcactattca tccgtttaaa gatggtagca atttttaatt gctcctcgtt
     2581 taaattaatc tcatcagctg tgactttatt gaccatcgca ctatattgtt cgctgttccc
     2641 taattgcttt tcttttgccg aaagtctttt tgtggcttct ttcaaatgtt tgggatcagc
     2701 cagccaaaaa tcttttttat ctctatctgt cagattttca tctaccggaa catcgatcag
     2761 cgagttcagc ttcgttgcga tttcaagcaa atctttagcc gtaacttgtg ttcctctcgt
     2821 atatgtgatg gcctgattcg ctttattcgt aactaaagct tttcctgtgg agtcgtagat
     2881 cattccgcga ggagtacttt cttggaccgt aatcgtagaa gtagacttca ctcgcgcctc
     2941 catctcttcg ccgttcacga tctgcaaata acccaatcta gcaatcaacg caacaaataa
     3001 cgtaaatata atgaaaaaaa gaaaatttaa gcgaaaagga acatgggacc ggcgaaacgt
     3061 tttgttctta ggttcttgtg caccttttct tattttttct aaccaagaag tatttaatat
     3121 ttttttcata aaatctttca tcatttccta tccttccgcc tatttacatt catgcttcat
     3181 tgtacccaaa aacttcttaa taaacaagaa gtatttaata tttttttcat aaaatctttc
     3241 atcatttcct atccttccgc ctatttacat tcatgcttca ttgtacccaa aaacttctta
     3301 ataaaaaaga aaaaatgggc ggagcaagaa attctttacc taatttcaag ataaaatcaa
     3361 ctttttgttt cacagcaatc acttaaagtt acatatccaa ctattttttt cattaaaaaa
     3421 taaacttatt tttgaaaatt atgttttctt aagtcgtgaa acactttgat ggatcattta
     3481 tttgtggaaa actacgtttt gttattctaa aaaacttata taaaaaatag taaggttttt
     3541 atttcgaatc cataaaatat tcttggattg cttctcgtgt ttttttcact ttttcgctaa
     3601 gcttttcgtt tttagccata tagaaataaa aaatgatatc gatcgtagca aattgggcca
     3661 aaagtgaatt agttgccgca cttcgattcg tgatttcttt tggtctagat gtttgtacga
     3721 gtacatcggc tatcttgcta agcgggttat ttccaaattg cgtcaacgca atgatttcga
     3781 tattcatgct tttcgccagc tcggctaatg cgactacatc tgcacttcta cctgagtttg
     3841 aaacaagcca aagcactttc tttttctcat tgctgacaag ttgcgggagc aagacgtgga
     3901 tatctttttc aaaaatgatt ggcttaccga cacgagacca tttttgcaga atatcttcta
     3961 ctactaaaga agaggcgcca accccatata aaaaaacagt ctctgccgat tccaaacact
     4021 ctacgacttt ttggatcgtt tcttcttcta gaatatctac tgtttctttt atcgctactt
     4081 gtgcattgtt accaagtttg tttttgatag agtaaaagga ctcatttgct tctatatcac
     4141 taaaaccttc atcgattcca gtctgtagtt ctgttgaaat cgtgattttg aagtccgtca
     4201 gactttgaaa accgattttt ttggaaaaac gtacaactgt cggcgcactc aacccggaag
     4261 cttctgctaa ttcattagct gtcattgtag ggaccttctc aatattaccg ataatgtagt
     4321 tggccaactt tttttctgct gaagaataat cttccatcat actttcaata cgactaatta
     4381 cgctcataag ttcctccacc cttttaatga tagtctatgt aagtatacca tatcagaccc
     4441 ttattcacgg tcgtatactg gataaaaaac aaaaaaacaa tccgcccaac agaatatctt
     4501 atcccatcaa tcccacagat tctattgagc atctgtgtct ttgaggaata cctgttatct
     4561 attgttcgga ttgctttcta tctactactt ttttacagga aatttaccta ttagttttct
     4621 ctgctgtaat ttttgacttc tgcttccata gcatcaataa agatattcaa atcttctaca
     4681 gctgtttctt tacttccata acgacgtacg ttcactgttg catcttccaa ttccttgtct
     4741 ccaacaacta attggtaagg gatcttttgt gtttgagagg cacgtatctt atagcccatt
     4801 ttttcattac gatcatcaac ttctacacgc aaccccttca tttgcaaacg ttctttgatt
     4861 tcataagcat aatcgccatg tgcatcaaca gaaacaggga tgattgttgc ttgtataggt
     4921 gccaaccatg ttggaaaagc gcctttgtat acttctgtta aataagcgac aaaacgttcc
     4981 attgtagaaa caattccgcg gtggataact actggacgat gcgtattttc cccgtcttct
     5041 ccaacatatg tcaaatcaaa gcgctctggc aataagaagt ctaattgtat agttgataat
     5101 gtttcttctg taccaagagc tgttttgact tgaacatcta gttttggtcc gtaaaaggct
     5161 gcctcgcctt ctgcttcaaa gtaatctagt tctaattcat ccatagcggc tttcagcatt
     5221 gcttgggctt tttcccacat tgcgtcatca tcaaaatatt tatctgtatt gtttggatcg
     5281 cgatagctca agcggaaacg ataatctgta atattgaagt cagcatatac tgccaccatc
     5341 aattcaagcg tacgtttaaa ttcatctttg atttgatcag gacgaacaaa tgtatgacca
     5401 tcattcaacg tcatttctcg tacacgttgt aaacctgaca aagcacctga tttttcatag
     5461 cgatgcatca ttcctaattc agcgatacga attggtaatt cacgataaga atggatcgtg
     5521 tttttgtaga ccatcatgtg atgagggcag ttcattgggc gaagaacaag catttcgcca
     5581 tctcccatat ccattggcgg gaacatgtct tcttgataat gatcccagtg tccagatgtt
     5641 ttgtacaact caacatctcc cattattggt gtatatacat gttggtaacc tagactaact
     5701 tctttgtcta cgatataacg ttcgatcgtg cgtcggattg ttgccccttt tggaagccag
     5761 aatggcaaac cagaacctac ttctggggaa accatgaaca agtcaagttc ttttcctagt
     5821 ttacgatggt ctctttcttt tgcttcttca cggagacgga tatattcttt caaagctttc
     5881 ttgtcaaaaa aggctgtccc gtatacacgt tgcatcattt gattgtttga gtttccacgc
     5941 caataagcac ctgccactga taataattta aatacttgta tacggccagt ggatggaaca
     6001 tgtggtccac gacacaaatc gatgaattcg ccttgttgat aagcagtaat cacttcttct
     6061 tctggcaatt cactaatcag ttctactttg tacggatcac ttgcaaaaat ttctaatgct
     6121 tcctgttttg aaagaacacg gcgttcgatc ggcagatttt ctttgacgat tttcatcatt
     6181 tctgcttcga ttgccggcaa atcttctgca gttacttggt tttgtccatt atctgtatcg
     6241 tagtaaaacc ctgaatcgat agctggtcca acgccaaagt ggatatttgg atagaggcga
     6301 cgcatggctt gggccatcaa atgagctgct gaatgacgga ctaagccaag tgcatcttca
     6361 tggtctggtg tgatgatctc aatacttgca tcttcctcga ttggacgagt caaatcaacg
     6421 agttttccgt tcactttacc agctaaagct ttttttgcta agctttttga aatgctttca
     6481 gcaattgcca atgttgttgt tccagactca aattctttga ctgcaccatc tggaaaagta
     6541 atttttatca taatagtttc ctccttgaaa tgttttgatt gatcagatca tcaagctgaa
     6601 aaacaaaaaa atcctagcat ctcttgaaaa gagatactag gacgaaaagt tcgtggttcc
     6661 acctaatgtt caaacgcaaa tgcgttcctc aagcgttgta acggtccgac cgcccttgct
     6721 tcaacaaggg tttcgaaagt ggtcatcctt tggttttctc ctggaaaagt ttcagcctag
     6781 cttttcctct cttatcgaag actctcaaat tcagttggct ttctcatcaa tcaattatgt
     6841 gtttatttaa ccactaataa atagaaaaat caagtgggaa actaaactta ttttttccat
     6901 tccccattta caccgtatgt tccttttttc atctcattga tgatgacatg gatgtgttct
     6961 tttggtgcct gcgtgttttt atgaacagct tccgtgatat ctttcatcat attttctaat
     7021 tgttccggac tgcgaccttc tactaattcg acatgtacga atggcataat gctctctcct
     7081 ctttctgatc gttctttttt caactcgatt ctatcatact atgtttgtct tgggtactga
     7141 atcaggaaaa atggctggat ctttttttgc tctcaaggca ctcaaaatag agaccgtatc
     7201 cacgacttct tgcaaaacag ctccgaataa tgcggggatt agtcccgtac ttgcaattaa
     7261 catcaaaaaa atacaaataa agatgccaat cagtacagac tgtttcgcta ttctcatcgt
     7321 atctcttgaa atcactactg cttttgctac acgcaagaga tcatctttca agatgaccgc
     7381 atctgcactt tcactagcag ctgtagaacc atgagccccc attgcaatac cgatatctgc
     7441 agcagcaagt gctggtgcgt cattcacgcc gtctcctacc atcgcaacag gtctttgatc
     7501 tttagggata ctttctaaaa tggtcagttt atcttgaggc aagctacggg catgtacaga
     7561 atctaattgg actttttctg caatcgtttg agcaatggca gatccatccc cggtgatcat
     7621 catcaaattt ttcacatcaa gttttcttag ctccttgatt gtctttgctg cttctgggcg
     7681 aatctgatcc gaaaatagga tcgttcctgc aaaagatcga tcaattgtta catagattgc
     7741 tgtattggac gtttctattt cttcagctga aacaaaagag gcacgtccaa tctttacttc
     7801 atgattattg actactgctt ggattccttg accggtcact tctttcagtt gactaacagg
     7861 atacagttgg ctgtttttca cgttgttcac taacgaacga gctaagatat ggctagattc
     7921 ctgttctgca ctagctgcat acagaagtag ctcttctttt tttattccat tgactggctg
     7981 aatcgcctct acttccaata tccctttggt aagcgttcct gttttatcaa aagcaatcgt
     8041 ttttgtacgt gcgagctttt caatcgttgt tccagttttt acaacgatcc cgtttttact
     8101 tgaacggctc attcctgcaa ctaaagcaat gggcgcagct agaatcagtg gacaaggtga
     8161 tgccactacc agtacctcag caaatcgtaa agggtctttt gagaaccacc aagaaatacc
     8221 accgatcaaa taagcaatca acgtgaaagg gactgcataa cgatcagcta aacggacaaa
     8281 tcgggcaggc ttttcttttg attcttcgac tagtttaata atcatttgat actggctgtc
     8341 tgcggcacgt ttttcagcga tcatttggat cgatccgtct ccatttaaag accctgacat
     8401 aatctgatct ccaggtttct tggtttccgg gcgggactca cctgtcaaag aagattcatc
     8461 gaccgaagat tctcctttgg tcactcgacc atctgcgggt acaagttcgc caggcttgac
     8521 tagtaactgg tcatcaatat ttatttcatc gattactata tcctgcaatt ttccttcttt
     8581 gaatcgatgg gcaattcttg gggtgttatc taacaaagac tgcagttctt tacccgctcg
     8641 tttattcgcg taatcctcta gactatctcc gcccgtaagc atgatcaata tcatcaaact
     8701 agcccagtat tctttgacgg ctaatgttgc aaggattgcg gtaatcgcta atacatctac
     8761 gccatatctt cctgaacgta acgtcttgat catttccaaa aacatcaaga aggcgagaat
     8821 ccctccagta aatgcgacaa tcgcaaatgc aagggccggc tggtgaaaaa caaattcact
     8881 taataaagct atcccaccaa taagcaaagt aagtaaaaat tttttgaaat gtgtcattgt
     8941 tttttctcct cttttatgtg acttagagag gttagtaata aatttccagc tgcctctttc
     9001 atcttaagca tagtccttga ctagcacttt ttaaaacaat aggccttaat tgaaaacaac
     9061 tatcactatc agcgtttcca aaattcatca gttctacaaa caaaaatatt cagcaaggat
     9121 tactttttcg attcatcagt taaaaagtac cttttttcga aaaactatgc tatactttta
     9181 attcgttatt tacggcggaa ggagcaaaca tgcgatgaat gagaaacaac tagaacaaac
     9241 gcacgtggat gaaacgatcg aactaatcca gatcgaacaa aatatgctgg atacaaaaca
     9301 acaagaattg actcaggaaa tgaatcattc gacaaaagac acagctaatc acaagattcg
     9361 agctggttcc aatgaagctt tttatgaatc tgctgttgaa tatcgccaac atgagcagga
     9421 attgcttttg aaataccata ctcttgaagc acagcaaaaa cgcctgcaaa cgttgtctgt
     9481 catgaaagat agtccttatt ttgcccgtat cgactttaaa gaagaccgtg aaacagaaac
     9541 attatatcta ggtatcgctt ctttaagaga tctggcagaa gaaacaatcg ttattgactg
     9601 gcgcgcacca attgccaatc tttattatga aggtgagatt ggtcccgcat tttatgaaac
     9661 tgaaacagat agaatagatg tcgatctgca gttgaaacga caatttaaaa tccaagaagg
     9721 acgtatttta tctatggtag atacgtcgga agtaatcaat gatgactttt tgctagagat
     9781 attggatgaa tcatcaagca accatatgaa aaacatcgtc tctacgatac aaaaagcaca
     9841 aaatgcaatc attcgggaca caacaagtaa agtaatgctg attgaaggaa tcgccggaag
     9901 tggaaagaca tccgctttgc ttcaacgtat tgcttttctg ttgtatcata atcgcaagtg
     9961 gctcgaggct gaaaacgttc ttctattttc acccaaccat ttgttttctg attacatctc
    10021 gactgtattg ccttcattag gtgaaagcgg cgtgcctaca cagacattca aaaattattt
    10081 ggtgcagctg ctgccagagt tttctttact agatgaacag caacaagaaa gtggtttttt
    10141 gctaggggaa aaagatccga tacaagtgat gaaaagcgga ttgaccttag tcgatcagat
    10201 tgatcgctat atccaaggca tcacaaacta cggtcccttg ttccgtgata tgaaaataaa
    10261 tggccgtacg atcctatcaa aggaatcgat tcgccaatgg tataaagaaa caaatgaaca
    10321 attaccttta catcatagac tttctctatt acaaacgaag ctcctgaaaa aattaggcgg
    10381 tctgcaaaaa gacgaaacac gtcagcaatg ggtcaaagat ctggcggaag aacagctcca
    10441 agaactatac gcaacagatc caaatcttga atatacagaa caaaaggagc gttcattacg
    10501 taaaaaactg gccaaccaaa tcgtacgtaa aagattccgt cgaatccatc gagggatcat
    10561 gaattatcaa tttgtcaata taacgaaaca atatctccac tttctacaaa ctgtgccact
    10621 ttctcttcta acacgacata aaatcagcga gaccaggtgg caggaacatt tgacaactgt
    10681 tcgtacacac ttgcgtcaaa gagaattgcg tcaagaggat gctgtactat tctttttact
    10741 gatgcgccga acatatccag tgacggttac acacaaagct cgctatatct ttattgatga
    10801 aatgcaggat ttcgcaccat cacaagtggc attgctgcaa gccttgttcc caaaagcaaa
    10861 tttgactttc tgtggagatc taaatcaaaa tgtctttgga aatgaaacga tcgttggatc
    10921 attggaagac cttttcgtgg accgtgaagt cactcgtttc caattaacga caagctatcg
    10981 ctccacaaaa gaaatcactg attttgctaa tcattttatt gtgaaagaac atcaagtaga
    11041 aacaacagca agaaaaggag accttccttt gctcatccaa ggaacaacag atgatatcaa
    11101 attgaattgg ttagcagata ccattcgaac gactcaggga aaagcccggt attggcgtac
    11161 agcaatcata gctaaaacac aagaagaatg cgaagctctt tatgctcaac tgccagacga
    11221 gttgaaacaa caagtccagc tgattttaaa tgagagtgac tttatgaagc gttccattat
    11281 tttacttcca gcctttttag caaaaggtct ggagtttgac cgagttttct tgtgggatgt
    11341 agatgcagaa catttttctt ctcagcaaga caaattgatc ctttacacca tgtgtacacg
    11401 tgcgatgcat gaattgaccc tttttacctc caataatgaa agtcctttgt taaacaatat
    11461 ggatatttca tcttatgaga aaaaagtact gtaagta
//
LOCUS       DACEXQ010000074        11406 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.074, whole genome shotgun sequence.
ACCESSION   DACEXQ010000074 DACEXQ010000000
VERSION     DACEXQ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.074"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(518..1336)
                     /gene="purR"
                     /locus_tag="IXH04_001388"
     CDS             complement(518..1336)
                     /gene="purR"
                     /locus_tag="IXH04_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAQ1852004.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(1503..2327)
                     /locus_tag="IXH04_001389"
     CDS             complement(1503..2327)
                     /locus_tag="IXH04_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ1852005.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(2300..2980)
                     /locus_tag="IXH04_001390"
     CDS             complement(2300..2980)
                     /locus_tag="IXH04_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ1852006.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(3003..3941)
                     /locus_tag="IXH04_001391"
     CDS             complement(3003..3941)
                     /locus_tag="IXH04_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAQ1852007.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(4186..5037)
                     /locus_tag="IXH04_001392"
     CDS             complement(4186..5037)
                     /locus_tag="IXH04_001392"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAQ1852008.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(5191..7764)
                     /gene="mprF"
                     /locus_tag="IXH04_001393"
     CDS             complement(5191..7764)
                     /gene="mprF"
                     /locus_tag="IXH04_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ1852009.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            7905..8711
                     /gene="proC"
                     /locus_tag="IXH04_001394"
     CDS             7905..8711
                     /gene="proC"
                     /locus_tag="IXH04_001394"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAQ1852010.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(8790..10346)
                     /gene="rny"
                     /locus_tag="IXH04_001395"
     CDS             complement(8790..10346)
                     /gene="rny"
                     /locus_tag="IXH04_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAQ1852011.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(10757..>11406)
                     /locus_tag="IXH04_001396"
     CDS             complement(10757..>11406)
                     /locus_tag="IXH04_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA recombination/repair protein RecA"
                     /protein_id="HAQ1852012.1"
                     /translation="GAVDIVVVDSVAALVPRAEIDGEMGDSHVGLQARLMSQALRKLS
                     GSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYATIRLEVRRAEQLKQGTDI
                     VGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDMAVEKDIVDKSGAWYSYKE
                     DRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAVPEDEKGQEELPLVEE"
BASE COUNT         3348 a         2512 c         1942 g         3604 t
ORIGIN      
        1 ataacgtgcg tccaactgcg tcactcctaa atatagtttg ctgatttcag tttatcccag
       61 tatacccatt ttttcaacaa aacaagtgat tttcttttat cccagcgttt tctggataaa
      121 aaaatagata gaccataacg ggcctatcta tttggtaacc ggtgctctcc tgccaactcc
      181 tcggtcacaa gtcacactac tcataaaaca tgaaaagctg ttttttgaaa tcgtttcttg
      241 tgacttagag ttaaacggta ggcttacaac ctcttcatta aacgtttttc aatcagctga
      301 ccttcggtat tagctcaata aacggcaaaa tatgaggagc aatttttccg tttattgagc
      361 taatactcag gacaggacgc tgatttcaca acctctagat aaacggtgtt caaaagccag
      421 tttctcggta ttaagccaaa tttatgaaaa aatgtggaaa gccattttca caaaattttt
      481 cttactactc gaaactaagc agctttttca caacctctta ttctgaaaaa taatttcctg
      541 gcacaaccgt aatatttttc gtttgagtat ctacatcttt gacataaagc agtgacgtat
      601 aatcatcgat ggcgcgatga ccgttgaatt ttgattcggc aaatacagtg atgccaacaa
      661 gttctgattc gaattcttca atcaagctct tcattccgtt gactgttccg ccgcctttca
      721 tgaaatcatc cactacaagg acacgtgaac cacgtttcaa acttctctta gacagttcca
      781 tcttttcaat tctttcagaa gatccagaca catagttgac acttactgtt gatccttctg
      841 ttatttttga gtcccggcgt acaataacga atggcgcatt cagataatag gacacagctt
      901 gggcaatagg tacgcctttt gtggctacag tcattacagc atcgattttt tcaccaaggt
      961 atttagaagc aatgattctt ccaacttgac gcagcagttc aggttctcct aacaaatcag
     1021 agagataaac atatcctcct ggaagcagac ggtcttgttc tgatagacgt tctgctagag
     1081 attcaatata ttttttcgcc tcttcatatg aaatttcagg gatgaacaat acaccacctg
     1141 cagcacccgg gattgtttcc aaggttccat accctcgttc tttaaatgtt ttcttgatga
     1201 ttcccaaatc ttcactgata gaagatttag cagattcata acgttcggca aagcttgtta
     1261 aggaaatcaa ttcatgcggg tgattcaata aatactgtgt catgtcaact agacgttcac
     1321 tgcgacgtac tttcacctat tccaactcct tttaagaaat tttatcgttt tacattatat
     1381 aagtttttct tcaaaaattc gagcatttta tgaaaaaata agaaaatagt tcggtattta
     1441 ttaaaataaa aagaagccta taaaaaattt ttcgttttta taaggcttct tttctttata
     1501 atttagttat tttttcttct gaaggcaacc aatattcgtt tgatgatatt tacaatcagg
     1561 aacaaagcaa taaagactaa tgtgattgtt gctcctggag gtgtatctag ataaaacgag
     1621 ctagttagtc ctgacagcat ccctagaaat cctacacaaa cactgatagc aatcacgaca
     1681 ttgaagcttt tgcctaatcg cataccgatt gctgcgggaa gaaccatgat tgcggatatc
     1741 aaaagtgcac cggcaatcgg gatcatcaca gcaatagcca caccagtaat cacattgaac
     1801 atcatcgaca tcaaatgaac gggtagccca tccacgtggg cggtatcttc atcaaaagtc
     1861 agaacataca tcggccgttt gaataaaaag aacaatatac cgatggcaac gaataagatt
     1921 cctaaaaaga tgacttgctc ccatgtaatg gtcacgatcg aaccaaacag ataggattgg
     1981 atacttgttg ctgaattccc tccgctcaaa ttcatcaata ctaacgctag cgccaaacca
     2041 ccagccatta aaatggcaat ggagatttct gaataggttc ggtagatcgt tcgcagatac
     2101 tccaatatca cagctgctaa tacaacgact agcatggtcg taagtgtagg attaaggttc
     2161 aaaaagaatc ctaacgctac ccctgctaaa gagacatgag aaagcgtatc agccatcagc
     2221 gattgtcttc tgatcactaa aaacacacct agcatcgggg cgattccggc aatgaagata
     2281 gcggcaagaa acgctcttct catgaattca tatgaaagca acgccattga gaatcctcct
     2341 ttctcaccag tcggatctgt ctgtccgcat actgtttgat atcttcatga tcatgggtga
     2401 tcattagaac tgatttccca tggacatgcg cactatgtcg gagaagacga taaaattcat
     2461 ttctggattc ttcatccata cctgttgttg gttcatcaag aacaaataag tcagggtcag
     2521 tagcaaaaat acgcgctaaa ctgattcgct gcttttgtcc gccagacaat tcacctattc
     2581 gccgatttcg catatcccac atccctacag cttctaaagc acgctgtaca tgttgatgat
     2641 ctttttcaga gagacgcttg aaccagcggc ctcttggata tcttcctgaa cggactaatt
     2701 cgatcaccgt actaggaaaa cctgcattga aagatgcaac ttgttgagga atataaccga
     2761 tacttaattt ttttccttct tgatttttct cagcaatctt tatcattcct tttgaaggtt
     2821 tcagtaatcc taacgatgct ttcaccaagg ttgacttggc tgctccattt tctccagtta
     2881 aaatgacaaa ttcacccgca tctacatgat aagagacatc ttctagtaca ggttcatcat
     2941 catagtaaaa ggtcaaatca gatatttcga tataacgcac cttacaacct ccattctaaa
     3001 acttaattga tactttcttt taaagctttc agattttttt ccatgacaga aatataattt
     3061 tcgcctgctt tgatttgttc attagtcaag ctttctaaag gattcaacac ttctagcttc
     3121 actcctgttt cattcgacaa tgtctcagct accttcgaag aggcattttc ttcaaaataa
     3181 atcactttga tgttgttttt cttcacaaat tcttttagct cagctaagcg acttggtgtg
     3241 ggttcctgat ctggtgaaac gcccgaaatc gctacttgtt ttaagtcgta ttgattcgct
     3301 aaataagcaa aagcagcatg ctgagtgaca aatgtgcggt ttgttgcatc ttttagctca
     3361 tcggagtatt tttgatccag tgcttcaagc tttttgatat aagcatcggc attcttcgta
     3421 aaagtttcct ttttatctgg atatttttcg cctaattctt cagcaatcgt tctgacttct
     3481 tttatagcta ataccggatc gagccaaaca tgtggatcgt attcatgtga atgaccatgc
     3541 ccttcttctg attcttcatg ttcgtctgct tcttgcgatt ctaaacggtc gattccttta
     3601 gctgcttcga tcatcaaggt ctgtttgcta tccactgaat cctttaacga atccacaaaa
     3661 aattccatgt ctgaactatt atacacgaaa acatctgcat cggaaatttc tgctctttct
     3721 tttgcacttg gttcgaaatc atgcggctcc gttccagcag ggatcaaaag tttcacgttt
     3781 ccttcatccc ctacgatctc ttttgtaaaa tcatacatcg gataaaaagt cgtaacgatc
     3841 gtcaaatcct cttttttgtc tgcttccttg ttcgtatttc cacatgctgc aaacagtaaa
     3901 gcacctatca ttaccgtagc tgtcaaaaga tattttttca tttatatgct cctttcaatg
     3961 aacaaatcgg aattttacga tttattaacg tgaactaatg tatcaaaata aaaaaacaca
     4021 gtcaatagaa atccatcaat ttctgattta agcatgtata ttcttgatta aatcgtaaaa
     4081 tggatgagtt gctgctcata tctagccatt tcactacaat aaaaaactga agaataccat
     4141 tttaggattc ctcagttttt catcacctat tttacttctt atattttatt gcagggttcg
     4201 tactaaataa acttcctcac aaaaaccttt taacccatta tatactctct gcgctcttga
     4261 ataatgttga cacaaggcaa aaacggttgg tccgcttcca ctcatcaatg cagcatctgc
     4321 tccgtacttg atcatacgct ctttgatttg atggatgact ggatgccgct ttcccgttat
     4381 atcttctaaa ctgtttccca tttttttgat catctgctga taatcttgct ctcggatggc
     4441 ttcagcaagt ccgtggatgt cttgatgttg gatggcttgc gtatctactt ctctaaacac
     4501 tgtacgtgta gagacactca ttcttggttt gaccaacaca acccagcact gtggcataga
     4561 aggtaatact tggatttctt cccctttgcc accgacaaat gccgtacttc cgtagacaca
     4621 gtaaggaaca tctgtaccga cttccaaacc gatttctgcc agttcttcta acgaacagcc
     4681 cagattccat aattggttga tccctcttag agcagctgca cagtctgtac ttcctccgcc
     4741 taatccagct gctactggaa tattcttctt aacaaagatc ttgacaccgg ttttgatatt
     4801 gtatttattt tttagcaata aagcagcttg ataaacattg ttccgttcat ctaccggtaa
     4861 aaatgcttta tttgtttcta caatgatttt gtcttctgtt atttcttcaa aaaatagatg
     4921 atctgccaag tctacgctcg acatcaccat ttccaactca tgatacccat cttgccgtct
     4981 atataataca tccagtccta aatttatctt ggctggcgct ttttcaatca attccatcct
     5041 ctcacccatt cgaaaagtct aagtcgcaag tattctatca tagggaaaaa taaaagtata
     5101 gcttatgtct gcctagtggt tgattttgtc atcgtttcaa cagctttcac agaattttct
     5161 cttcataatc caaacaagtt ctagtgattc ttataattct gtgagcactt cttctgccaa
     5221 actcggaaca tttccttttt cttttcctat cagactgagt agctgcatgg tagcaaatcc
     5281 gaaattactt cctttcctat aagcaaaata acgtccttcc caatgatcga catattttaa
     5341 tttcttttca taagcagact ggaaggaata gaaaaaatta ccataattat atacgatatg
     5401 catgactttt tcttgaaaga aagaaaaatc tgtttctcca acattagcta atggagctaa
     5461 gcctagattg gcttccgagt agcccatttt ctcgtataca ataaacagat tggctaacag
     5521 atagtcagct aattcctcag attctttagg taagacccgc aacaaatcat atcccgcctg
     5581 cttcttcgtc atcggctgag cggtgatgaa gccaacgact ttttgatctt tacggacaat
     5641 accgacaccg ctagataata catatgattt atcaaatcgt cccacagaga agtatttttc
     5701 tcgtgcacct tgaagccatt tttgtgaaac ttcatttaca gcttgatgta attcttccgg
     5761 cagttgatcg taccaagtaa aatggtatcc ttctttcatc aaacgttgat attcaagttg
     5821 ctgactcagc cgagacaagt cttctgttgc tagatcaaca atccctgctt cacctacttt
     5881 cgtgaagtga aaacccaatt catgaagcat cactacgtag ctttctgata tcttataaaa
     5941 cgcaagctga tagcctaaac aatctgcttg tttcataaac tgattcgttg ctgcccgcat
     6001 cattttagga ttgccaactg gatctcctaa aacaaagcat ttgtttcctt tgatctgata
     6061 accaaaaacg acttgatctt ggtcgtcttc ttggtaataa aaataactgt aacctgacaa
     6121 gcgaagctga tggcttgtag ctgtgcctcc gtatgtttcc aataaacggt caaaacggtc
     6181 tttttcgtac tcactgccaa gcttcgtagt atcttcagct aaatattgat aaagcgccac
     6241 taaagctagc aatgagatgc ccaaaccaat aagccctgag aaccacacat catctgaagg
     6301 aaacaaaata aaatgatttc ctgttattcg accgactaaa aagcgtccgc tgtgatagcc
     6361 tgcaattgcg taaacgataa acaaaaagcc gaacaagcta gcatcaaaga ttatagctcc
     6421 ccaagaataa acgaattttg ttcgataaaa ttcttttctg gataaccaca cagctaacag
     6481 aatcaagaca tataccaata tcaactgcca agataacgta cgtgtgattg tatttaaaat
     6541 accaaaagat agtaaagcaa tcgtaggcag aaatgccttt ttgactttta ctgaaagtgc
     6601 tctagcaagg ccgagtaata aaaagccaac caatagattc actgtctgat ctaaaaaatt
     6661 gaatgagaac ggcaataata cttgaaatag ccgactgatt gcagacagat tagtgattgt
     6721 tgatagaagt accatcatga tccccgcaaa atagacagca cttacgagca cgatatgcgc
     6781 aaccttttgg aaaaacagtc gcggaatgtt atctaaaaat cgattgattt tgactcctgt
     6841 atgtgtgatg aacaatagaa gtcccgtcat aaaaggtaga acataataaa acaaccgata
     6901 atataatagc caaactactg ctacatcttg ccgaacaggc aattgactca ttcctaagat
     6961 catcaaaacg tcaaaagtcc ccattccacc tgggaccatc gtcagcattc ctatcaatgt
     7021 agcaatcatg aacataggat agatcgaaag catcgaaata tctattctca tcaacttgcc
     7081 gatgatcaaa aataccgcaa gggctcccgt ccattgtcca aaggatgcca gtagcagacc
     7141 aagcaagcct tttggataaa actcgaagaa caatgtcttt ctctttagat aagcaaaaaa
     7201 gaaaatgact ggtgctaaaa aactaccgcc gaaaagccag atccaatagc ttcgaaaaat
     7261 actttctgac tgaataaaaa agacatcaat aaatgtaaga aacgaccata cagacaaacc
     7321 agtcagcata aatagtgcga cttttgatac agtggctaaa accatttttc tatttgtctg
     7381 ctttccataa aaactagccc gcagacttgc tcctaccaca ccaccaaaac cggcgagatt
     7441 gttgatcgtg tttgttaccc acgcagcaag aagaaactct tttctttcca tttttggttt
     7501 tccttgtttt tctaagactc tgatcgtcac ccaatcatac aacaacatcg gagtgacacc
     7561 cagcagtcca gccagaatca tgagcaggat cgtcatgcgg ctttgttgtc ccatcctttg
     7621 ccaaatatct tgccaactca tgccattagc aatatttgcg acttgatttg ccacaaagac
     7681 taacacggag ccgaaaaaaa tcagttttaa aatcagacta tgttccttta tccattgcag
     7741 catcaagtat cgtttatttt tcacattttc ccattcctaa cttgtatact tttgtgtcat
     7801 accccaatgt tatcttattt attttaattt ttccacttta gacctgttct ttgcttgcga
     7861 tgatagaaaa agaaagatat cctaatgcta aaggagagga taacatgaaa gtaggattta
     7921 tcggcacagg gaatatggca caagctatta ttgatggttt attaaaaaaa ggaacagtct
     7981 cgaaagaaga ggtgttcgtt tccagtggac attttgaaaa tgcacaaaaa tttgctgctg
     8041 aaactggcgt ccatgcatgc aaaagtaatc aggaagttgc atcacaatca gatatcgttc
     8101 ttttagccgt gaaaccaaaa atcattgaaa aagtattgga agaactaaga gaatatagcc
     8161 agaaagtctt attcatttct attgcttcag gaaaatcatt aacagattta gaaacagcgc
     8221 ttgaagatcc tcaggcctcc atcgtacgtg tgatgccgaa tgtcaatgtc agcgtaggag
     8281 caggcgtttc tgcgatttgc aaaaatgaag ctgccacgga ggaacagctt cagcaagcta
     8341 cgatgatttt tgaagcaatc ggcagcgtct atcctttagc agaatcagat ttcagtacat
     8401 tcattggcct tgctggcagc tcacccgcat tcgtttacat gctgatcgat gcgatgggac
     8461 ggactggtgt actgaatggt ttgtccaaac agacagccac tgagattgct gcaaaagcaa
     8521 ttatgggttc ttgtcaaaaa ttcctttcaa gtgataaaaa tccatgggaa cttgtagacc
     8581 aagtttcttc tcctggcggt acaactgttg caggtgtagt tgccttagaa gaagcaggat
     8641 tcatcccagc aatcatcaaa ggaatcaata cgacgatcga gaaagatctt gagatgttga
     8701 ataaaaaata atgatagcaa tgttttttcg tgccttacaa aaaaagaaga gctgtggctt
     8761 ttgccgcggc tcttcttttg tattttttat tatttagcat aatcagttgc acgagtttcc
     8821 cgaataacga ttactttgat gtgtccagga taatccagtt cttcttcgat tcgtttacga
     8881 atatcacgta ctaaacgaac agcgtctaaa tccgagattt cttctggctt gaccattacc
     8941 cgaacttcgc gtccagcttg aactgcgtaa cttgattcta caccggcgaa gctgttcgaa
     9001 atattttcca gacttcttaa acgacggatg tagttttcta atgattcact tcgcgcacca
     9061 ggtcttgctg ctgagagcgc atctgctgca gctacaagga cagagataac agaagtagct
     9121 tccacatctc catgatgaga agcgatcgcg ttgattacta ctgggttttc tttgtacttg
     9181 gcagctaatt ccgcaccgat ctcaacgtga gatccttcaa tctcatggtc aagtgctttc
     9241 ccaatatcat ggagcaaccc ggcacgtttt gctaattgga tatcttcgcc aagctctgct
     9301 gctaagatac cagctaattt tgcaacctcg accgaatgct tcaagacgtt ttgtccataa
     9361 ctagttctga atcgcagacg tccgatgatc ttgattagat ctggatgaag tgtatgagca
     9421 ccgacttcaa acgctgcctc ttccccgtat tcgcgaatac gttcatccat ttctttacga
     9481 gatttctcga ccatttcttc gatacgcgct ggatggattc gtccatcttg tatcagtttt
     9541 tccaaggtca tccgtgcgat ttctcttcgg attggatcga atccggaaag aacaactgct
     9601 tccggtgtat catcgatgat taaatcgata ccagtcagcg tctcaagcgt tcgaatatta
     9661 cgaccttctc gtccaatgat acgacctttc atttcatcat tcggaagggt aacgactgaa
     9721 acagtcgttt ctgaaacttg atctgctgcg cagcgttgaa tcgctaatga taataagttt
     9781 ttcgctttgc gatctgcttc ttctttggca cgctgttcag attcttttac cattagagtt
     9841 aactcatgat tcagctcttc ttcagtcgat ttcatgatga catctcgtgc gtcctctttt
     9901 gataaaccag caattttttc tagttctttt tgttgatctt caatcatttt ttcaacgtct
     9961 ttttctcgct cttcaattaa ttgctgtttt gaactaagtg cttcttcttt actttcaagt
    10021 gagttctcgc gtttttctaa cgagtcatct ttacgattca gcgtttgttc gcgttgaatc
    10081 aaacgattct cttgagattt taattctaac ttactttctc tcagctcact ttcaacttca
    10141 gaacggtatt tctgattttc ttccttagct tctaacaatg cttctttttt tagagtttct
    10201 gcttcttttt ttgcactatc gatgattcca gcagctgatg attgtgcacc agctatttct
    10261 ttctcgtgac gtgactttgc gaccataaac cctaaaccaa gaccgacaat taaaccgatg
    10321 atagcgagga gaatgttgaa taccataaaa tccacctcca tactatcttt ttcattttta
    10381 tcatctatac agcttctatg ataaattatt ctaaactcgt caatatgcat cttcgcatat
    10441 atgtgtacaa aaaacatgca caactttatt ctaatgtttg taccccgtag tgtcaacttt
    10501 aaataaacaa agaaacggct ttctacttat tcattaaacg atggttttta aaataaatga
    10561 cactagtttc atataggtaa acctttaaat tgagaaactt ttctattaaa ttattctttg
    10621 tagatgaaca gatattttta ttttatgtac ccactcgtat aattaaataa ctcacgattt
    10681 ctttattcaa taaaaaaacg gcaggtcaag gagagtacct actcttcaga ccagccgttt
    10741 ttgtttatca taatttttat tcttctacta aaggcaattc ttcttgtcct ttttcatctt
    10801 ctgggacagc gacttcttca ccaataccat aagctgctct aactagagca gaaacttctg
    10861 ccatcatttc aggatgatta gccatataga ttttagcgtt ttcccgtcct tgaccaatcc
    10921 gatcttcttt ataagaatac caagcaccgc ttttatcaac aatgtctttt tctactgcca
    10981 tgtctagaag ttctccttct tgtgagatcc ctaaaccata catgacatct acttcagcga
    11041 ttttgaaagg aggtgctact ttgtttttta cgactttgat ctttgtacgg ttaccaacaa
    11101 tatctgtacc ttgtttcaat tgttctgcac gacgtacttc caaacggatc gttgcgtaga
    11161 atttcaatgc gcgtcctcca ggagtgattt ctggattccc aaacatcacg cctacttttt
    11221 cacggatttg gttgatgaaa atagcgattg tttttgtttt gttgatcgaa ccagagagtt
    11281 tacgcaatgc ttgagacatc aaacgtgctt gtaacccgac atgtgagtca cccatttcgc
    11341 cgtcgatttc tgctcgtgga actaaagcag caactgaatc gactactacg atatctacag
    11401 ccccac
//
LOCUS       DACEXQ010000075        11198 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.075, whole genome shotgun sequence.
ACCESSION   DACEXQ010000075 DACEXQ010000000
VERSION     DACEXQ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11198
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.075"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            154..674
                     /locus_tag="IXH04_001397"
                     /pseudo
     CDS             154..674
                     /locus_tag="IXH04_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(576..1292)
                     /locus_tag="IXH04_001398"
     CDS             complement(576..1292)
                     /locus_tag="IXH04_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAQ1852013.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(1342..1551)
                     /locus_tag="IXH04_001399"
     CDS             complement(1342..1551)
                     /locus_tag="IXH04_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852014.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            1828..3101
                     /locus_tag="IXH04_001400"
                     /pseudo
     CDS             1828..3101
                     /locus_tag="IXH04_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(3208..4239)
                     /locus_tag="IXH04_001401"
     CDS             complement(3208..4239)
                     /locus_tag="IXH04_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1852015.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(4246..5076)
                     /locus_tag="IXH04_001402"
     CDS             complement(4246..5076)
                     /locus_tag="IXH04_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1852016.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(5073..5879)
                     /locus_tag="IXH04_001403"
     CDS             complement(5073..5879)
                     /locus_tag="IXH04_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1852017.1"
                     /translation="MKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLANYS
                     DIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLAANF
                     AGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYPSMK
                     EVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALTSSN
                     VNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(5885..6709)
                     /locus_tag="IXH04_001404"
     CDS             complement(5885..6709)
                     /locus_tag="IXH04_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase"
                     /protein_id="HAQ1852018.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(6699..7799)
                     /locus_tag="IXH04_001405"
     CDS             complement(6699..7799)
                     /locus_tag="IXH04_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ1852019.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCVSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            7977..8762
                     /locus_tag="IXH04_001406"
     CDS             7977..8762
                     /locus_tag="IXH04_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HAQ1852020.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            8795..9178
                     /locus_tag="IXH04_001407"
     CDS             8795..9178
                     /locus_tag="IXH04_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAQ1852021.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            9254..9385
                     /locus_tag="IXH04_001408"
     CDS             9254..9385
                     /locus_tag="IXH04_001408"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1852022.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            9354..9590
                     /locus_tag="IXH04_001409"
     CDS             9354..9590
                     /locus_tag="IXH04_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1852023.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(9668..10639)
                     /locus_tag="IXH04_001410"
     CDS             complement(9668..10639)
                     /locus_tag="IXH04_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAQ1852024.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
BASE COUNT         3345 a         2584 c         2134 g         3135 t
ORIGIN      
        1 cctttagaaa aaaggcttct ataatatttt gtgttgttgg aaaatcgaag gcgattttct
       61 actaacactt tttcttcgtt tcaactcaaa aagagactca cttttggtaa aatttagtcg
      121 accaaaacat caccctacca aaggagtcgc tctatggata atcatactag aaaattactc
      181 ggattaacag ataaacattt attttttgat gaagaatggc tgattgaaaa agaatataaa
      241 ggcgttcaag cgcagtttat taaagggaga ttggatgact caccttccca ttgtgaacac
      301 tgtggatcga ttcgtattat ccgcaacggt tcctatacga cacgtactca gatgttgaaa
      361 gtcaaagaaa agttgactat tttagaatta aaacgtaccc gttttctatg ctatgattgt
      421 gggcagacct tttctgcgaa aacggactta gttgatgaac atcatcaact aacaaaggaa
      481 ctgaagcagg cgattctcat agaactgtac gaaaatcaat cacgtaatct gaaaatcaga
      541 agaagtaccg tcggctcaag cgatattgga aactcttact caaagattcc acgacattgg
      601 agccgctcaa acggcattat catcgactgt ttaaacgacc aattagtcaa acagaaatcg
      661 tcgatgaacc gttgtgcaca atggtgtcct gaacgtacag cgacaccttc catatccaga
      721 gcagtcgcca catcacgggg atggatgcca tccaaattaa aagcaaccac gccagtatga
      781 tgttcaggat cttgcggtcc gtaaatggtg ataccgccga tcgcttgtaa tttaggtaaa
      841 acgtaggcaa ccagttcttg ctcataacga tgaatattct ccataccaat ctcattcaag
      901 taatcgattg ctcctgcaat gttcggagta ccagcttcaa atttccatgg cagctctttc
      961 catgaactat cgtaaagatt gacaaaatct atcatttcac cgccgaattc gactggctcc
     1021 atttgttcta acaattgacg tttgccatat aatacaccga ttcctgtcgg cccgcacatt
     1081 ttatgcccgc taaaggcgaa aaaatctgca tccaacgctt gtacatcaac tgtcatatgc
     1141 ggtgctgctt gggcaccgac tggactatcc tcaactacaa cagcctgttc aaccctcagc
     1201 cgtttcaagc acaacaaata accttctgga tctggtttgc cgacctgaag atcatcgatc
     1261 gtcacaaagg aatcaatttt ttccttaatc atcggatgtg cttcaaaaaa atagtctagc
     1321 tcccgcagtt cagaattgag actactccga ttttccagtg ctgctgatcc gctgctcaaa
     1381 atgttcagca tccactggtc tcccaaccgc ttgacttatc ccttcataaa aggacagttc
     1441 tgctgccgtc ccaacacctt gttgatagtc tgcaaaagga attttatagc cgaactgctc
     1501 cagtaaatag gcttcagtaa atagtgatga atactctcgt aaaacatcca ttcactgtca
     1561 atcatcgtcc catcaaaatc aaaaataagt gctttggtca tcttctttcc cccttaccca
     1621 aaacaagcaa aagctgtgtc ctgctctctc tacagtatca gcataaaaac tgccgtctga
     1681 aaagtcaaag gtgaaagaaa tgtggcgcta taatttctag gactgatagt aaatcattct
     1741 atcagtcctt ttgagcataa aaaaacaaaa catacagaag ctccgttaga attgaatcac
     1801 cacaacacaa tcaaaggagc gttctgtatg cctgatcttc atcttatcaa actattacta
     1861 ggaatcaaag atggaaacat tcaaatcact catgtcgaga agaaaaaaat acattcaatc
     1921 aacagtatcg ttgtttctgc cacgctcatt aaaacgattc agccctgtcc tcagtgtaaa
     1981 caggtgaatc aacatgggct gatcgttaag aacggaaaga aaaaatcgct ggtccttctc
     2041 aataagtgtg cgaatcaacg gacttatctt gctttggcaa agcaacggta cctctgtcgt
     2101 ggctgtcaca cctattttac ggcgaatacc tacattgttg aaaaaaactg ttttatcgcc
     2161 aaacaagtac attataaaat attggaggaa ttgactgaaa aacaagcaat gacgacgatt
     2221 tctaaacatt gcggcgtttc ttggtcaacc gtttcgcgaa cactcgcttc tctgctcccc
     2281 atgacaaacg tgaaaataaa ttggttgcct cgctgttcat tggtagatga atttcgttca
     2341 ttgaaaaatc aagtcggtcc ctattctttt tcttgtatgg atggcgatac tgggaaatta
     2401 ctggatattc ttccttcacg taagaagaaa gatctcattt cttattttat gcaatttgaa
     2461 cgacgtgcac gtttaaacgt aaaaatactc gtaacagata tgaacgcctc ttatgctttg
     2521 ctgataaaag agtgctttcc tcatgccaaa ctcgttgtgg atcgctttca catcgtcaag
     2581 cacctcattc gcagttttaa ggatatttga ctatgagtca tgaaaagctt cgaccgaaac
     2641 gatccaatac aagccaaaca ctatcgacaa gcgaaggctt tatctcgact tctgatcaaa
     2701 cgacaggaca cgcttgttta tgataaatgg acaaaatggc gtaattttgg ttgggcctat
     2761 ttaaccgaaa gtgaagtcgt agaacgtcta ctttctactt ctgatgaact acgaatagct
     2821 tatgcttact atcaagaaat tcttcaagct ttttatgaca aagaagcgga catcttcttt
     2881 cagcttgtaa aaacaatgcc caagtcggtt ccaagagaac tgcatcatat aaaaaaagcg
     2941 tttataaact atgaatccgg cattcgttta gccttagagt tgccttactc aaatggcaaa
     3001 atagaaaatc tgcataccca catcaaagca ttgaaacgaa tagcttacgg gttaggatga
     3061 gaaccagaat tttcttaatc aatcatctga tcacttatta aacataaaaa aacatctaaa
     3121 gcagaaaatc aattctacct tagatgttct tatatcactc tatcagtcct atttgacaaa
     3181 gagccagaaa tgtttgccga ctatacacta gctttgttgt gcaaaatgct gagtatcaat
     3241 ttccacaggg attgctagaa tatctttcag ctgaatgtat aaggcatcgc taattttctc
     3301 ttcccgatcg ttcaaccgat ccactaaaat ttctttgccc tccagcgttt gaaaaacata
     3361 ccgttgaatg ttgcctaaaa taaatgattc cttccataga accggaatct taaccccctg
     3421 aggaaccggt tcaaattgaa tcagctccgg tcttaaataa taaatgcagc gattgccctt
     3481 agcggaaaac tcgtgggtat actgattcag ctcataccat tcgaaacgat tatagttgcc
     3541 gataaattcc gcaacaaatc ggctctgagg attctgatac aattccttcg aggtggaaac
     3601 ctgagccacc cgcccagctt ccatcacaaa aatccgatca ctgattcgca tcgcctcttc
     3661 ttggtcatga gtcacaaaaa tcatcgtcat ccctagcgat tgctggatag cgcgcaaatc
     3721 ccgctgcagc tgttttctaa ttcgggcatc caaagcactt aatggctcat ccagcagcag
     3781 gactttcggt tcagtgacca atgagcgagc cagtgccacc cgttgtcgtt gaccgccaga
     3841 aagaaactgg acatttttat ctgcctgctc cgtcatatcc accaatgata gcatctcaaa
     3901 aaccttttcc tgaatcacct caggactcag cttctttacc ttcaaaccaa aggctacatt
     3961 ttcaaaaaca gtcatagttg gaaacaatgc atactgttga aaaacaaagc cgatattgcg
     4021 atccttcgta ggcttatcgg caatattctc attgtccagc acaatctcac cgctctttgg
     4081 ttgttccaat cccgtgatac agcgcagcaa cgtagacttt ccgcacccgc tgggacctag
     4141 taaagtaacc agctcaccct tttcaatctg aatttcatca atagctaaaa tctgtttttc
     4201 atcaaaaaac agattgagat catgtatttc taaaaacatt ttccgtcaca actcctttaa
     4261 gggcttcttt ttcgactgct tctcttccct cgtctgctta agggacagct gttgcttttg
     4321 tcgataagtc agcataaagg tagttaaagc aattcccacc ataaacagca aatacaaaac
     4381 catcacagcg ctagccaaat ggccgttttc gttcatccgc cgcagcatat agatgcgtac
     4441 cgtctgaaat cgaccgccaa tcaacaggtt cgtcagcatg tactccccaa aaaccgaaga
     4501 aaaaatcatc agagaaacta gggtcacacc gatacggatg tttggaaaca gcacctggat
     4561 aataatcgta gacatcgggg cgcccaaggt ttctgctgca tccactaatt ccttcagatt
     4621 aattaagcgc atctggttgt taagactaag atagacaatc ggcaacgccg taataaacaa
     4681 tgcacctacc aacactacaa acattgggat accagtttca gcatacgttc gaagcaaagc
     4741 cgttaccaaa atcactcctg gtaaagcata cggcagcagg agcagctttt ccagccaacg
     4801 attcaaacga gggaaataca aatgaatcca aataatggtg ggaatcatca acaacagaat
     4861 cgtcaccaag accacacctg ctaataaaag tgagcgaccc accgcagcaa taaattccct
     4921 gtccgtcagc agctgttgga accattgcaa ggtaaaatca ctaggtaaaa gcgattttga
     4981 ccatttagtg gagctggcat acaaaatcgt tccaataatc ggcagcaagt aatacaggac
     5041 aaagcagaac aaaatcggtt ttattaactt tttcacgtct gcaccacctt gcctttcttt
     5101 tgaataatca acattgccgt taccagcagg acacctaaag cgactgccaa cgcactgcct
     5161 aaattaggct tggcgaacac atctcccgac actaaagccg caattcgaat cgttaaaaga
     5221 tttacattac tgctggtcag ggcatacgct gtttcatagg tccccatgcc attagcaaat
     5281 aagatgacaa aggtgctggc aatcgatggt cgcaaaaaag gcaaagccac cttgaaccaa
     5341 gcatacgctg atgaagcgcc cagcaattgt gcggactcca gccattcctt tttcacttcc
     5401 ttcatgctgg gatacaaaaa caagacccct aacggaattt ggaaataggt atacgtgatg
     5461 gtcaatcccc accaagaata caaattgaag tccgctagaa atggcaaaat cattttcagc
     5521 acacctacat tccccaacaa aataataaaa gagaatgcca gcggaatccc agcaaaatta
     5581 gctgccaaat tggaaacaaa ggtgattttc tcctgcaatt tagtcgatag attcattaag
     5641 cataaacata aaataaacgt cccgattaac ccaatcacac tggagctgac agcaatcagt
     5701 acgctgttaa caaaggcctg tgtgtaatat ttgttgctga aaatatcact gtaattggcc
     5761 aatgtccact ggttagttaa atcactgtgc aagctgcaca gaaccataaa aagcagcggc
     5821 agcagtaaaa atgcacagaa gataaaggag gcaggaacca gaaccaagcg ttttttcatc
     5881 tctttcatcc cctcatcaat tcttgaatgt cctgcatttt ctctgacggt gcaattccta
     5941 ataaccagca aaacaatgga gccgccagca gctgtggtac agtttgttcc ttcagctttt
     6001 ggcgtttatt tgaaatcaaa taaaaaggaa cttcacgatg tgcagccaag ctgccgccat
     6061 gcagtccgtg cgcatccatc ccgtgatccg cagtcaccac gacttgataa cctttttgca
     6121 gccattcagg aaggtaaata gccaataatc tgtccgcctg attaaccgct gctgcatatt
     6181 catgagaatc ggcggtaaat ttgtgaccga catcatcaat attcatcgaa tgtacgacca
     6241 aaaagtctgg ctgatactga ttcactaaat aatgggcatc cgcaaataaa tgcgaatcag
     6301 ggtaagtgtc ctcactataa aagatgccat gctgaatcag tcgctcctcc tgatgctgga
     6361 tgcgatcaac caaacgatta tagggggctt gattgtacaa ctcgctgtac cagtggtaag
     6421 cggcggctcc tgtttttccg ccagcttcct taactaattc aaacagcgac cactctttgg
     6481 atcgctgagc atggtaatta ctatagatgc cattttcata ggctggaacg ccggtcatca
     6541 gtacttcgta taacggacgt gaattgctgg gcagctcact ttttaccttc aataagcttg
     6601 cttgctggtg ctcaactaaa tgattcatga acccaagctg ggcgacggct gtgtcgtagc
     6661 gacagccgtc tagtataact acaatcagtt tgtcaagtct actttgcata ggtcagtacc
     6721 tccgcctgcc aaagctctgg aagctctaca gaggtcttgt tccaagcttc attgtcttca
     6781 actggcaccg ccgcttcata agcatcctcc ggcagtaatt tttcagctac ttctttaggc
     6841 agctctacct tgcgaatagg tcgcgcatag ccctttgcca aattgatttg cccttcatca
     6901 gataaaatat attcccgtgc tagctttgcc gcattgggat gaggggcatt tttattgatt
     6961 aaggttgtat acccgctaat gatggagccg tctgttggca cagttacctc aaaacgctcg
     7021 gagtcaattt ggtcacgata atttagtcca ttgaagtccc aaacaactgc cacatcgatt
     7081 tcaccttttt caagatttgc tacgctggca tcaacggtgg acaaacggcc tttttctgcc
     7141 atttttttgt agtaattcag acctggctga atatcctttt cgtcacctcc gttggcaatc
     7201 gccgcagcca atacagcaaa ttgtgcttgg ttggcaatcg tcacatcccc cacgctaact
     7261 tgatagtcac cctcttgcag ctccttccag ctagtcggtg cttgcttcac attctcttta
     7321 tcggtgataa aggaaatggt tcccgtatag cttaacaacc aatccccatc atcatccttt
     7381 gcccaatccg gaatttcctc ccaatagttg gtcttataag gtaaggttaa ctctttgctt
     7441 tcagccaacg gaccgaagct gataccgaca tcaccaatat cggcggttcc attttttcct
     7501 tctgattcaa acttagccaa ttcttctgca ctggacatat ccgtatccac gtgacttaag
     7561 ccgtattctt tttcaaggtc gttccaagtg cctacccaat ttgcccaagt atccggcatt
     7621 cccaagctgg caatctctcc ttcttccttg gctttttcta taatcgtatc taaagagtcg
     7681 tctgccgcag ccgattgctt cgttgcttca cctgagacgc agcctgtcag caggatactt
     7741 actgttgctg cacaaaataa accgatagtc atgttacgaa ttttttcatt cattttcatt
     7801 cattcttctc ccctttcgat aattccattc taacaaagct ttgtaatgag agaatcagtt
     7861 tcttgaaaag gtttcatgaa caatgtaaag gttgtatatg gtttgtaaat ttcgacgaat
     7921 tcccgttaac cttctaaagc ttttgcggta tgatagtaga aaaaaggagg ctccctatgc
     7981 agtattatga tcagcatctc cacacgtatt tttcctttga ttcagaggaa aaatttgaaa
     8041 actatttagc ctatcagccg gagtttttcg tttctacgga tcactttgat ttaaacaatc
     8101 catctaccgg aaaagatgac attccggatt atccagccta tataaacaaa ctggactctt
     8161 taaaagaaac ctattccacc aaatttctgc gaggtatcga gattggcgtt gttccaggac
     8221 aagaagagcg gatcgtggat tacctcgctc agcatcctta tgatttaaag ctggtaagta
     8281 ttcatcaaaa tggccgcttt gattatatgg acgatgtggt tttaaccaaa gatcccattc
     8341 aaactaccaa ggaatatttt gatcagatgt tgcaggtctt gacggatttt ccagaaggag
     8401 atattttgac tcactttgat tatggattac gccgcttcac cttcacagcg gaagaattcg
     8461 cggagcattt tgaggctcag ctgctaacga ttttcaaaaa ggttgttgaa cgagaattgg
     8521 cgatggagtt aaatgccaag agcttcttga aatacggcaa cgaagacctt taccgttatg
     8581 ccgttccttt gtatcaaagt gtcggcggaa agctctttac cttaggctcg gatgctcatg
     8641 ttgccgaaga ttatcagctg ggctttaaaa aaatgagtca gctgttggca gactgtcagg
     8701 tagaaaagtt gctagtcatt caaggaaaag aacgccgcct gacgccgctg ccgattttgt
     8761 gattcctata taatagaaga aagtggtgaa aaaaatgagc aacctgtggg actacaatca
     8821 agaggcaccc atccattatt taattgcgcg acattgggat gcgttgaaaa ttgaggcggt
     8881 ttgccgatcc ttgttagcag ccgttccaaa acaacaactg gaaaattttt tggtggctga
     8941 cagcttgcag cgggaaaaag ttcaggctta ttttgcagcc tttaaagatc agcctttgga
     9001 atatttgcat gcgcagtttc atctttttta tcaagtagcc gccccagatg actacaacga
     9061 tttacgagga cagctgcaat tgacctttca agcggatgaa accgcctata ccgttttatt
     9121 gggaatggct cgccttggtg atcaggcgaa ggtcgagtgg cggattttcg atatttgatg
     9181 gcaggatcaa ggcgtttcag ttgcaaacct tcctctcttt caattagaaa aaccgcagca
     9241 ggaagaagtt tgtgtgctgg tcaccgatgc tggtgaaatt cgtctccgct tatttcctga
     9301 ccaagctccc ctggctgtag ccaactggcg ctccctagct gagcaaggcg tttatgacaa
     9361 cacacctttc ggctcgtgtc attaaggaat ttgttattca aggtggcgct ttggatggtt
     9421 ctggtgatga agctgtttcc agctatgagg gctttttcgc tgatgaagtg catcgcggac
     9481 tttatcattt taatggcgcc ttggccttag gggaccacgg cccccacacc aacggcaacc
     9541 agttttttat tgtgcaaaac acaaaggcgc aggctgacct gctaatgtaa agcaagcata
     9601 cttggagctt ggaggactac cggagctgga tggtcgttat actgtttttg gacaggtata
     9661 ccaagggtta gaaatcgtag cagcgatcgc agctcagccg acaaacagtg aagatgtacc
     9721 gattgagcca gtgttcattc gacaaattaa cttcgaaaaa acatcgtaaa aagacactca
     9781 ctgacttcag caaaagctct attcttgagc tggctgcagc tagtgagtgt ctttcattta
     9841 tccataagat aaagcttgga ttcaataaat aaataaatgt tttggttaaa gctgtgacca
     9901 gcaaatgagg aaccgtgcga gccagttgat ctgccaatgc aaacatttct ttaggtgaag
     9961 gcgctggcgt gtctgccaac ggatactcca gtcccaatgc ttggtaaccg tgcataccat
    10021 agcagtggta cggtagtaat tctaacggta cgaaccgatc aagatctgcc acaaaacaag
    10081 caatcgtcag cagctcggtc aggttcatat tgatacctgg aaccacagga actcgaattc
    10141 gcacttcgca gttaggatac tcaattaaaa agcgacgaat attcttcaaa atttgttggt
    10201 tatctaagcc ggtccattgt ttgtggagct tgctggtaaa catttttaca tccacaaaca
    10261 atgtatccac atagggagct accttttgga tttcattata agcaccacaa aagctggttt
    10321 ccagtacggt attgattcca atgtatttcg atttttgcaa aatttcttta gcaaaatccg
    10381 cctgaaccag tgcctcgcct ccgctaatgg tcactccgcc atccgagtga aagtaaaaaa
    10441 cagcatcctt ctcaatctca tccattacct cttcagcggt catggtctgg ccaaatcctt
    10501 tttcaatctg cttgctttgt gattcgggaa tcgaacacca ggcacagcgt aatgggcagc
    10561 cctttaaata aacaacggtc cgtaaaccag caccatcatg tagagaaccc tgttctattc
    10621 ttaatactgt tccttccata tagatttttg aattacacac tagtttaaac taaaacacac
    10681 aaggaaaaac ttaccattta atcataaaat tagctcatat aaatatacta ggatgtaaaa
    10741 tagactggtc aaaatctggt caaaaaagag gactaataca ggtagtcctc ttttttgtta
    10801 ttttaatatt ctaaatcatt ttttattggt ttattccctt attcgatgta gactcaaatc
    10861 actatatcac tagatatcaa acggcgtcat acggaatcaa aaacatcaaa tttgacctgt
    10921 taaatggtca ttgaataaca ttcaaaatca ataaaacgga gtcagacacg gagtcaaaaa
    10981 cgctgagagt aaaaaatata tacagccttg taggtaaagt accacagggc tgtatatttt
    11041 ttatttcaga ttaccttaaa tgattccatt tactttttat ctcatttttt cctactagtt
    11101 agtatcaaat actctcctat tttctcaata aactattttt ggatacatgg tatctgaaac
    11161 agctaaaacc ttgtcattac agcaaaaaat cacgttat
//
LOCUS       DACEXQ010000076        11081 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.076, whole genome shotgun sequence.
ACCESSION   DACEXQ010000076 DACEXQ010000000
VERSION     DACEXQ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11081
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.076"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            53..313
                     /locus_tag="IXH04_001411"
     CDS             53..313
                     /locus_tag="IXH04_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAQ1852025.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(1312..2547)
                     /locus_tag="IXH04_001412"
     CDS             complement(1312..2547)
                     /locus_tag="IXH04_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAQ1852026.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            3115..3942
                     /locus_tag="IXH04_001413"
     CDS             3115..3942
                     /locus_tag="IXH04_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1852027.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            complement(4215..4985)
                     /locus_tag="IXH04_001414"
     CDS             complement(4215..4985)
                     /locus_tag="IXH04_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ1852028.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            5203..6270
                     /gene="ulaG"
                     /locus_tag="IXH04_001415"
     CDS             5203..6270
                     /gene="ulaG"
                     /locus_tag="IXH04_001415"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAQ1852029.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            6288..6749
                     /locus_tag="IXH04_001416"
     CDS             6288..6749
                     /locus_tag="IXH04_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1852030.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            6769..8253
                     /locus_tag="IXH04_001417"
     CDS             6769..8253
                     /locus_tag="IXH04_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ1852031.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            8296..8595
                     /locus_tag="IXH04_001418"
     CDS             8296..8595
                     /locus_tag="IXH04_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1852032.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            8610..9248
                     /locus_tag="IXH04_001419"
     CDS             8610..9248
                     /locus_tag="IXH04_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAQ1852033.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            9253..10113
                     /locus_tag="IXH04_001420"
     CDS             9253..10113
                     /locus_tag="IXH04_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAQ1852034.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            10103..10813
                     /locus_tag="IXH04_001421"
     CDS             10103..10813
                     /locus_tag="IXH04_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ1852035.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
BASE COUNT         3573 a         1738 c         2329 g         3441 t
ORIGIN      
        1 gagaaaacga agtccagact gtctaagttt ggtttttaga aggtgggaaa caatgtattg
       61 gctgatttta ttttttgttt ttatcttttt attaacggct agtcacctta tattgaatat
      121 gctagctaca tatcatattc aaatcaatcg atggatttgg gcactggcat catttttgat
      181 cgtgattctt ccaaagatca ttgtaccgca tatgaatgtt ttgttttcat gggggacata
      241 tgtactttgt ggaatttttg ctatcaattt tatgatcgag cagcatagat ggtttgtcac
      301 atcgaagctt taaatgttct aaaagtggtt gtaacagaag tggttagctt caagaaataa
      361 gaagaaattt acgaaaattg cttcttaaat ttttgtgaaa tgttggctta tttttcgttt
      421 ggttgaactt attaccgcag atgcaagctt ttgaccaccg tttattcgga atatgggact
      481 gtgacaaggc ttttgtcaca gccctttttt acataaagat tattattgtt ttaactgttt
      541 tggcagaaag aaggaggaaa aaatggaaat tttatcagca atggatttag aattagtaga
      601 tctactacca aagtgatcaa gtcaaatata gatttgggag cttacaacca tatagtaggg
      661 gctgcagtat ggtgtatgaa tggtaatatg tatgatgtga gtgtttattc aaatcatgaa
      721 acgtgtatag aaataattgt gctgggaata gcaatcagtc atggtgaacg tgacttttag
      781 tgtatcgttg ctgacgacat agaacattct gacatgatct actcaatatg tggggattgc
      841 tggcagttta tataggagta tgcatcggat tgcgagatca ttgtctcaac agaagaggga
      901 ggttgattat caaagataaa gtggtaaatc ctacatttga tttagtaatt gttgagggga
      961 ctagcggcac aaatctctac aaagcgatgc ttctagatct atatgacaca ttttaagaaa
     1021 atgtatttgc ctaattatta tggtctttct tttgaagaaa cgttgagatg atccaaccag
     1081 agaaaggtgt caaagaattt gactaagaat aaatggcaga cgctacctat gctcatttgg
     1141 atagcgtcag atcttttatt ttaaggatga ttttgtgttt tttagacacc gtaatttaag
     1201 taactctaac ttttcaatcc actatttact ttttttgtat tttcactgtc tgggtctcga
     1261 atagcgaaat tactgatgag tctctattaa aaaagctttt tctacttgaa actatattgg
     1321 gtggttgctc aggaaggttt cgatttcttg aattgcttgt tgtggaccat ttattggaaa
     1381 aacggcttgc agcgtgcgtg cctgctgacg aatcttgggt gaagaaactt cgcgcagagc
     1441 aatctgcagt tttctttcag tcactggttt tgtgagggcg attgcgtttc cgtattttac
     1501 acaatagtca atattacagc tttgctcaaa ttgcatcccc acaccgataa acggtttgcc
     1561 ggattcacag gcagtttgga cggtcccttc cccgccgtgg ataaaagaaa aatcaatgaa
     1621 ttcatgcaaa agatgactag acagatattc tgttattaaa agatcttctg agtcagattg
     1681 aatgtcatca agcagatagt caataggtgc aataaccgct agatccggtt gtttttgtaa
     1741 gaagaacaaa attttgcgta acatacgcgg attgcctgaa ctacccattg cgaaatagat
     1801 aatacgtttt ctttcttgtt gctttcgctg tataaaagtt agcacttctt caggcaagga
     1861 agtactgagc ttagcaaaaa ttggaccaac gattcgataa ttttccggca gctgttcttc
     1921 ttccaaaaat agtttaggcg ttgtgatgag atttagatca gcatccatca tttgtaaact
     1981 tgtttttggt aaaggaagca tttctttttg tgcagcgtgt ttgaaggcat tggggatcca
     2041 tcgggtattt actatacgct tttttgtcca gtgccaaagg ggattgctta aagggccgaa
     2101 gaaacgtaac tcgtggggca tcagcgcatg ctctgattga aaaaaaggtt cactcaaagc
     2161 atagggttta acatatacta aaggaatctt tttattgcgg gccgataaaa aaattgtcac
     2221 attcgaacct gtaacaatca gtcgaggctc aaacttagca atcagactct gttcactagt
     2281 gatacgagag gcaaccattt ctttggtaaa agggttttta agtgtacgca actggtcaaa
     2341 ttttatgatt tgttcttctt gttttttcgt aaggatcggc gctagtaaat gatactcaaa
     2401 gccactttct gtgatcaaag gagcaaaagt tttagaaaag ccaaagaata cacagtaata
     2461 acggtctttc atagctttgg caacctcaat cattcgtgtt gtctcagcta aattgaatac
     2521 tgcaggagca aaaagaattg tcttcatgaa atcctcctta ttggaatgag tctatccctt
     2581 cgtttcttac tagtatacta aaaaacacag cgttcgagta gcctatgtat attttgaata
     2641 ttaaactgtc tctcgctgtt ttcacaactt ctaaatttaa attttgtaat caagttgaaa
     2701 ctccaatttt caaaaaaaca cgcaacaatt ttttgatcga tttatatgtt gtgccagata
     2761 aatacgagaa gttgtgtcat taaaaataaa catgagaaca taaaaaaata aaataattat
     2821 attaattgat aggtgtgctg tcgtcattat acacacgtaa gaaagaaata gttatgttta
     2881 aaaatattct tagactttct cgccatgaca attttcacta tgtgttataa ctgtaaacga
     2941 taattattca gataacacaa aaagaaatca aggagcagat acgccagttg tttaatttga
     3001 atattatttt aaaattaaaa tttttattct gttctaaaca tttttggttg tttagaactt
     3061 ttttttgtgt atgatataaa ggagaaataa ttttttaaag ggaaggaaat tgttttgatt
     3121 aaagtagatg gagcattact aaaagaatac cgaaagaaaa aggggttaac acaagtacaa
     3181 ttagcagaag gcatttgtga acaagcaact gtcagcaata tcgaaaacaa gaatatgtcg
     3241 ccaggcatca atatcttaag tgcgctttgc aaaaagctga atgtcgagat ctccgatata
     3301 ttagtagaaa cagaagaaga caaaatcagg aaaaaaataa atgagattca atcattgaat
     3361 tctgtatcaa aataccaaga atcttataaa attttacaaa cgatcgatca ggaaaaagta
     3421 agtaattctg atcttttaaa tcaaatttta ttttatcaag gactgcttga tcatctttat
     3481 gcgaaaaaac cagatgcagc attattttat tttaatcaag tgttggaaca aacgaccgaa
     3541 acagatatcc atcacttaca agcagctgca aacgtggcaa tgatctactt gtcaaaagga
     3601 gaattagatt ttgcgaaagt ttatgtggag cgtacactta aaatattgag tgagactgat
     3661 tttgataatc tgatggtctg cattgtatat tatgatatcg ccacctatta ccgtaaaatc
     3721 gaggattatg ataaagcaat ccaattatgt gagaagggta ttgagtataa taagaaacat
     3781 aaatcgactt atgcattgga atacttgctt tatgaaattg cttcttgcca taagcaatta
     3841 ggagaagatg attatttaga aagatatatg gacgcaaaaa aaatagcgcg tttcaacgga
     3901 aatgattacg cggtaaaggt gattgaaaac gacctgaaat aaagtggttg tgaaatcagt
     3961 gttctgattt gaataccgtt tattcgaaaa aaggaggtgt gacttttgtc atacctcctt
     4021 ttttcgatat tgtttgattc ctatttaggc aacatataac ggttataaaa gaagcaatag
     4081 attatgatgg tatcgattgg atggatacat cttgggattc ctttatcaat ttgagcgtag
     4141 cgtgtttttc atttatcgat aaatttgtta ttcatttttt tgataatcct atttacatat
     4201 ttacgtcctg gttcttaaaa tgaatctact tgaatggttt ttatccccgt atttctgatc
     4261 tcctctaata ctttttcaga acaaaattta tcagtgatca aataatcaaa gactgaaata
     4321 tcagcaatct tgaactttga tgtcagtccg atttttcgat gatcagcgac taaaatctta
     4381 catttgattg tcttgttgac catcatttcg ttgatcgttg attcatttaa tatctttgtt
     4441 gtgaccccat gtactacatc gactccgcta cagccaatga tacaaatgtc tgcattgact
     4501 gaagaaatcg tatttaatgc gatgtcgccc actagtgctt cttttggaaa gcgcatctct
     4561 ccaccagtta aaatataact acaattatct ttgtgcggag agtaattgat tttcacgttg
     4621 tttgtaatga ctgtcaaatg gttagattga agaaaatcca gtgttttcaa cgcagtcgag
     4681 ctagaattga taaacagcgt atcataatcg ttaatgtatt tactagcttc tctggcaatc
     4741 gcttgtttaa tcctttcagg accagattga tcaaaatcag tctggttttt gtaattataa
     4801 gaacaataac cgtaattgcg tatgatctcg tttttttctt ctaataaatt taaatctctt
     4861 cgtatcgtac taatagatac atcaagggat tcagctaatt cgaaagttga tagttgttca
     4921 gtctgctgaa gcagttccaa aatttctttt tgacgttttt caatattgac aagtgagttt
     4981 ttcatgactt ttgcctcctg agggacatct tgatccatgg atttatttga ctagttgttc
     5041 atttaaacat tcatatctgt ctttattata ctgaaacaat cattgattac tgaaagaaaa
     5101 tcatataaaa aacaaacggt catataaaag ggttttcatg gatgctgata cacattaata
     5161 tttacttgta agttggaaca acgatatggg gggagcaagc aaatgagtaa aactgtcaat
     5221 gaagtaacaa aagaaaagtg gttagtagaa acatttcctg agtggggcac atacttgaat
     5281 gaagaaatcg aaacagaagt agtggatcct ggaacttttg ctatgtggtg gctgggctgt
     5341 acgggaattt ggctgaaaag tcatgaagga acgaatattt tatgtgatct atggtgtggg
     5401 acgggaaaac gtagtcatgg gaatggcatg atgaaaaaag gccatcagat gatgcgtatg
     5461 agtggcgttc aaaatatgca gcctaattta cgtactcagc ctttcgtaat cgatcctttt
     5521 gcgataaaaa atgtagacgc tttaatcgtt acacatatcc attccgatca tctagatatc
     5581 catacagccg cagcagttca tcaaaattgt cctgaagcaa cattcattgg gccaaaagaa
     5641 gtggttcgca cgtggcaaaa atggggaatt cctgaagaaa aaacgattac agtaaagcca
     5701 ggtgatgcag tcaaaatcaa agacatcaat gtagtagctt tagaagcgtt tgatcggaca
     5761 gcgttggtca cttgtgaaga tccagaagag gtattaaaag gaaaaatgcc tcaggagatg
     5821 gatcaaattg cagtaaacta tttattcgaa acatcaggag ggaatcttta tcatgcagga
     5881 gattcacatt actccaattt atttgccaaa catggtaatg aacacaaaat cgatgtctgt
     5941 ttaggggcct atggtgaaaa cccacgaggg ataacagaca aagttacttc tgttgatatg
     6001 ctgcgcatgg cggagtcatt gaatgcaaaa gtagtgattc ctgtccacta cgatatttgg
     6061 tctaatttta tggctgatcc aaaagagatc actgaaattt ggaaattcaa aaaggatcgc
     6121 ctgaagtatg gatttaaacc ttatatctgg caagtaggcg ggaaattcat ctatccgaca
     6181 aacaaagatg atcttgagtt taatttttat cgaggatttg aagatgtctt ctcgattgaa
     6241 aatgatacac cgtttccgtc gtttttataa gaaagaaggg gagtttcttg ctgaaatact
     6301 tttttgacca tcaattgatt cattacccag ataaagaaat cacggattgg cgtgaagcta
     6361 tcgccgaaag ctgtcttgta ttgatcgata aggaaattat tgatgaaacg tatgtggagg
     6421 agatcatcca ttgtgtagaa aaatacggtc cttatattgt gatcgcacct caagtagcaa
     6481 tgccgcattc ttcagaaaaa agtgatggtg tttttggaac agctatttca tttaccaagt
     6541 tgaaacagcc aatccgtttt gaaagtgaag gggagaatga ggagaaaacg gctagtttgt
     6601 tttttacact cgcagcaaaa gatccagaag aacatctaga aaatatccaa aaattatccg
     6661 aattattaat gacagatgga ttggttgagc gtctcatctc tacgaactcc atcgatgatt
     6721 accaagttgt gatggcagaa tataattaat tgatagagga gaaaaaagat gggggaattt
     6781 ttgttaagca tttggacgta ttttgcaaca aatatcttga cgcagccagc atacctgatt
     6841 ggttttatcg tgttattagg ctatattttg ctgaaacgcc cgcttcatga atgcatcgct
     6901 ggattcttaa aggcaacggt cggttacttt attttgactg ttggctctgg tggtttggtc
     6961 aataattttc ggccaatctt ggttggacta aaggaacggt tcaatttgga tgccatggtt
     7021 acagatcctt attttggaca aaatgctgtg gatgctggat taatggagac atttggccgt
     7081 acctttggag atgtgatgat tttattactg attgcatttg ttttaaatat cttattagtc
     7141 cgttttcaga aatatacgaa actgcgttcc gttttcacta ccggaaacgt tcaggttcaa
     7201 caagcagcta ccgctttttg gattttactg ttttgctttc ctgatctagg taggatacaa
     7261 gcattgattt ttatgggact gattttaggt tgttattgga cagtagcttc taacttaaca
     7321 gtaggaatca cgcaggaatt gacagaaggt gccggatttg cagtggcaca tcagcaaatg
     7381 ttcggaatct ttatatttgc aaaattagct gagtggatga aaaagcggga tgaaaagaaa
     7441 aatagatcaa tcaagcaaga caaaaaaatt gaagatatca aattaccagg attcttgtcg
     7501 attttcaatg agaatatggt tgctacttcg ttactcatgc tatttttctt tgggatcatt
     7561 ctgattgtac taggcaaaga ttatttgatt caagcgcaat tcatgcaaga agggcagagt
     7621 ttcttattct acattatgac gacttcttta aattttgcgg tatatctggc gatcttgcaa
     7681 ttaggtgttc ggacattcgt cgatgaacta actcaatctt tccaaggaat ctcaaacacg
     7741 attttaccag gagctgttcc aggaattgac gtagctgcta cttttggctt tggttcgcca
     7801 aatgcggtaa cgattgggtt cttgtttggt gctttgggac agtttttgat gattggttta
     7861 ctgattttat tgaaatcacc aacgatcgtc atagccggtt tcattccgtt gttctttgat
     7921 aatgccgtta tagcagttta tgccaataac cgaggtggat tcaaagccgc ttgcctgttc
     7981 ccgtttcttt caggaatcat acaagtaggc ggttcagcat tattcgctac atggattggg
     8041 atgtcaacat acggtggtta tatcgggatg ttcgactggg caacagcttg gccattgttt
     8101 acagtaatca tgaaagtcct aggttatgct ggaattgccg tagttgtcat tggtttattg
     8161 gctattccgc agttacaata tcggaaaaat cctgatggtt attttttgat tgtagaagac
     8221 tatgacacat atgcagaaaa gcagagagta taaaagaaaa tctgattaga caacactcat
     8281 aatggaggga ttaagatgag aattttagta tcgtgtgcaa atggatcagg aacaagcttg
     8341 atgatgatga gaagtgtgga aaaagcaatg aaagaactaa acgtaccaat taccaaaatc
     8401 catcattgtg ccatttcaga gggaaaaagt tcagctggcc aatacgatgt agtttttacg
     8461 ccagtcaatt ttttgaatat gtttactcag gcagagaaaa aaggagttac agttatcggt
     8521 atcaaaaatg tgatgtcagt caaagaaatc agtgaaaaat ttgctgaaac aaagtggtac
     8581 caaaaaaaca agtaaaggaa ggaactaaga tgagcaggcc gaatttgcaa attgcgttag
     8641 atcacaatga cttagaacat gcattaggag atgttatgaa agttggtgat gtagtagata
     8701 ttattgaagt gggcacaatt ctttgcttac aggaaggaca aaaagcgatt cgctgtattc
     8761 gcagtatgtt cccagataaa aaactagtcg ctgatacgaa atgtgcagac gctggaggaa
     8821 cagtggcgag taatgttgca aaagcaggag cagactggat gacagtcatt tgttgtgcaa
     8881 cgattccaac tatggaggca gcacaaaaag aaataggtga attgcaagta gaactctacg
     8941 gcaattggac ttttgaacaa gccatggatt ggcataacat cggtatccgg caggtaatct
     9001 accatcaaag ccgtgatgcg ctgttatctg gtgaaacttg gggtgaaaaa gatttatcga
     9061 agatcaaaaa actgattgaa ctaggcttta atgtttctgt aactggtgga ttaaatccac
     9121 acacattaca cttatttgaa gggatcgatg tgtatacctt tatcactggt cgtggtatta
     9181 cagcagcaaa tgatccgatg aaggccgcac aaaacttcaa agatgagatc attcgtatct
     9241 gggggtaagg aaatggctca aattgggatt tatgaaaaag cgttacccaa agatatttca
     9301 tggaaagaac gtttttccct tgtaaaagaa atggacttcg attttatcga gatgtcgatt
     9361 gatgagacgg atgagcggtt agcacgatta gattggtctg aagaaaagat agctgagctg
     9421 agagaagaga tgttcagctc cggtgtacgt atccattcga tttgtttaag tgcgcatcgg
     9481 cgatttccgt ttggttctgc tgatccagaa aagaaaaaag cagcgaaaca actaatgaaa
     9541 aaagcaatcc atttggcgca taatctaagc gtcaaagtaa tccaaatagc tggatacgat
     9601 gtttattatg aagagaaatc aatgaattcc agagaagact ttatcgaagg aataaaagaa
     9661 agcgtaaagg aagcttctaa atatggcatc attctttccg tggaaatcat ggatgatcca
     9721 tttatgaact ccatttcaaa atttttagaa atcaaagaac agattcattc accttttcta
     9781 caagtatatc cagacctagg caatttgtcc gcatggccgg aaaataatcc ggctcgagaa
     9841 cttgagaaag ggatcgatta tatcactgct atccacttaa aagatactta ccctgtaaca
     9901 aaagaatatt caggccagtt tcgtgatgtt ccttttggta gcggttgtgt tgattttctt
     9961 ggattattaa gacatttgaa acgtctagat tatgatggaa catttctgat tgaaatgtgg
    10021 agtgaaaaaa gccaagactt tcgaagagaa atccagcagg caaaagacta cttaagttct
    10081 aaattgaagg aggctggcta tgatgtcttg tgaagaaaaa attgcagcaa tgaagcaacg
    10141 tgtccttgaa gcaaatctgg cattgccgcg atacggatta gtcaaattga cttggggcaa
    10201 tgtgagtgaa gtggaccgtg agctaggagt catcgtcatt aagccaagcg gtgtcagata
    10261 tgaatgcatg caagcagatc aaatggtggt aacagatttg tcgggaaata taatagaaga
    10321 agatagctta aaaccatctt cagatctccc cacacatgtt gttttgtatc aaacttttga
    10381 agatataact gctattactc atactcattc cacgcactcc gtcatgtggg cgcaagcagg
    10441 acgcgatttg ccagcttatg gcacgactca tgctgatgct ttttatggta aggttccttg
    10501 tacacgccaa ttaacgaaag aagaagtaag agaagcttac gaagttcata cagggaatgt
    10561 gattgtcgag acgtttaagg aaagaaaatt agatccaaac gaagtccctg gagtacttgt
    10621 ctatggacat ggaccgttca catgggggga ttcacctatg aaagcagtag aaaacagttt
    10681 gatcttagat gaaatttgtc tgatggccaa agaaaacgag ctgatcaacc ccaatatatg
    10741 tgaaatcccg caatatttac tagataaaca ttattttcgc aaacatggaa agtatgcata
    10801 ctacgggcaa tagagatgct agggtaaggg tagaaaatta aaagataaca cttgatagag
    10861 agaaacagag gttgtgaaga aaatttatca caaccttttt atttatcaaa aagtatgcat
    10921 tctaaaagga aagaggaatt ttaaggaatc gagagaatct ttacaggaga gttatatgtg
    10981 atagattaaa tagtcaaaga actgtattat tagagggaga attgtataag aaaggaagaa
    11041 aaaaatgaat attgattccc ttatgatcga tgagatttct g
//
LOCUS       DACEXQ010000077        11037 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.077, whole genome shotgun sequence.
ACCESSION   DACEXQ010000077 DACEXQ010000000
VERSION     DACEXQ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11037
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.077"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            8..1258
                     /gene="lysA"
                     /locus_tag="IXH04_001422"
     CDS             8..1258
                     /gene="lysA"
                     /locus_tag="IXH04_001422"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008691072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAQ1852036.1"
                     /translation="MIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSKRFD
                     TKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKTPEE
                     LIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTAHID
                     SKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYLTDF
                     DQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPGRSI
                     VGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKTEKV
                     TIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFVNNG
                     QSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1245..2045
                     /gene="dapF"
                     /locus_tag="IXH04_001423"
     CDS             1245..2045
                     /gene="dapF"
                     /locus_tag="IXH04_001423"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAQ1852037.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2048..2929
                     /locus_tag="IXH04_001424"
     CDS             2048..2929
                     /locus_tag="IXH04_001424"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAQ1852038.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            2926..3642
                     /locus_tag="IXH04_001425"
     CDS             2926..3642
                     /locus_tag="IXH04_001425"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAQ1852039.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3648..4343
                     /gene="dapD"
                     /locus_tag="IXH04_001426"
     CDS             3648..4343
                     /gene="dapD"
                     /locus_tag="IXH04_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAQ1852040.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4359..5360
                     /locus_tag="IXH04_001427"
     CDS             4359..5360
                     /locus_tag="IXH04_001427"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAQ1852041.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEKPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5357..6718
                     /locus_tag="IXH04_001428"
     CDS             5357..6718
                     /locus_tag="IXH04_001428"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAQ1852042.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6678..7829
                     /locus_tag="IXH04_001429"
     CDS             6678..7829
                     /locus_tag="IXH04_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAQ1852043.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7804..8973
                     /locus_tag="IXH04_001430"
     CDS             7804..8973
                     /locus_tag="IXH04_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ1852044.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9148..10527
                     /locus_tag="IXH04_001431"
     CDS             9148..10527
                     /locus_tag="IXH04_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ1852045.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10808..11037
                     /locus_tag="IXH04_001432"
                     /pseudo
     CDS             10808..11037
                     /locus_tag="IXH04_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3656 a         1937 c         2483 g         2961 t
ORIGIN      
        1 gacagaattg ataattggcg gagtcacagc aagcacattg gcagaaaagt ataagacacc
       61 tctgtatgtg tatgatgaag aaaagatgag acaaacgatg ctgtcattca agcgaggctt
      121 tgtatcgaaa cgttttgata caaaagtgct ttacgcctct aaagcattcc aaacgattga
      181 gatgctgcat ttagttcgtg aatatggatt cgggctagat gttgtcagtg gtggggaaat
      241 ctataccgct ttgcaagctg attttcctag agatactatc tacttccatg gtaacaacaa
      301 gacgccagag gaactgatct atgcacttga aaatgatttg ttgcatattg ttgccgataa
      361 tttgatggaa gttgaattat tagcagaatt aagccaacgc tatttaaaaa aaatctgtat
      421 catgcttcgg ttgaatgtcg gagtagaagc acatactcat gaatatatcg tcactgcgca
      481 tattgattca aaattcggca tgagctatga aagtaaagag tgccaacaag cccttcagtt
      541 gataaaagaa agcagctatc tagagttaga aggatttcat gctcatatcg ggtcacaaat
      601 ttttgatgtt actgcttggt tagctgaaat cgacaaattg acgaattact taaccgattt
      661 tgaccaacct ctttccttga atcttggcgg agggtttggg attcgttata cacaaaatga
      721 tcagccgatc ccagtcgaag aggcactaaa aaaactgatt gaatatacgg aagaagcttt
      781 ggaaaaacga aaactgacca ttcgtcaatt actgatcgaa ccgggtagaa gtattgttgg
      841 agaagctggt acaacactat acacgattgg tttcatcaaa aagacaccac ataaaaaata
      901 ttactttgta gatggcggga tgacagataa tatccgtcca gctctgtatc aggcagaata
      961 cgattgtgat ttagcagcaa aactgtcgga agaaaaaaca gagaaagtga cgattgcagg
     1021 aaaaatgtgt gaatcaggcg atgtggtaat taaagaagct tatttgccga aagcagaatc
     1081 gggtgatctt ttagctgttt attccacggg agcttatgga tactcgatga gcagcaacta
     1141 caatcgagcg acacgaccag cagttgtttt tgtcaataat ggccaatcaa gactgattgt
     1201 tcgcagacaa gcattttctg atctactaag gggggaagtt tcctatgatc cttcataaat
     1261 ataatggctg cggaaatgat tttatcttat tagattacgc agaagacaca gattatagtc
     1321 aattagccgc ttctttatgc acaaaagaaa aatatgatat agacggattg atcgccgtaa
     1381 aaatcgatcc gttggaaatg atctattaca acaaagatgg cagccgcgcg ccgatgtgcg
     1441 gaaatggcat tcgctgtttt gctcgatacg taaatgatca gaacatgatg aatgaacagg
     1501 aattcaacgt agagacatta gcaggcacaa gaaaaattac ggtgttggat ccctcacctt
     1561 tttattgcag cgtagatatg ggagtgccag atgattctcc ggaaaaaatc aaagctaatc
     1621 aaacttatcc aataaaaaac caggaaatag agatcaatgg tgaaaaagta acgatcacta
     1681 gtttattttt aggaacgatt cataccgttg tttttgttga aaatgcgcgt gaggaagtct
     1741 ggagaaaaag aggtgaactg atctgtcatc atcctttgtt caaagaaaaa accaatgtca
     1801 actttgtaga agtagtcaat gaacaagaat tgatcgtccg tacctatgaa agaggagtag
     1861 gttggacact tgcttgcggt acaggttgct gtgcctctta tgtggttgct aaggaaactg
     1921 ggaaaatttc agcagatcag gtagttgttc acttggaaca aggagacttg atgatctctg
     1981 gtgaggatgc gatacaaatg gctggcccag cagttcatga atggacaaga atattggagg
     2041 aatgaagatg ataaaaggtt cgatcgtggc gctgatcacg ccgatgaatg aagagggatc
     2101 agtagattat gctggtttgg aaaaattgat ccaattccat ttagatgaac aaacagatgg
     2161 attattagtt ttaggtacaa caggagaatc ttctacgctc acacaatctg aggaggagca
     2221 gatccttcaa ctgacagtaa agaaagtggc tggccgtgtg ccggtgattg caggagctgg
     2281 aacgaataat acgaaagaaa cgattgaaaa agcgaagcat tttgcttctt tgggggcaga
     2341 cgcacttttg gtcatcaccc cttactacaa caaaacaagt gatgcaggac tagctgctca
     2401 ttttacagca attgcagaag cctcaccgat tccgcttatt ttgtataatg ttccttctag
     2461 aacaggcatg tcgattccaa tccatgtatt agtaaattta gcagaacatc ctaatatcat
     2521 tggtttgaaa gaagcctcag gagatatggc ttatgtgatg gatgccgctc ggttgattgg
     2581 agaagaattc ttcctttaca gtggaaatga tgatttgatc ctaccagtaa tgagtgtagg
     2641 aggaagcggt gtaataagtg tatgggcgaa tatccaacct aaaatagtcc atgaattagt
     2701 aaaagatacg caggacggaa gatggcagca agcaaaagaa aagcaattaa atgcacttga
     2761 actgatccat gccttgttca gtgagaccaa tcctattcct gtcaaagcag ccatgtcatt
     2821 attagactta ccttccggac ctctacgcct gcctttagtt tctctttcaa aagagaaaaa
     2881 gaaacaatta gctcagcttt tgttaaaaga aaggacggtt gaaaaatgaa aatcggactg
     2941 attggcagtg gacagatggg aaatagagtc gaagaagttg cacaagaaag aggagatacg
     3001 gtccttcttt tacggacggc acctaaagaa gcaactacgc ttgcttttac ggaactgcca
     3061 gaactgctta ttgatttttc tcatccagat aatttagaga aaatcctttc gtatagctta
     3121 tcatatcaac ttccgttagt cattggcacg acaggttata cacaatcaca gttccaagaa
     3181 ataaaagaac atgccaaaca tgtacctgtg atgtatagtg ctaatttttc gttcggcatc
     3241 atggtgatga atcaactaat caagcaagca gctgcaatgt tgcaaggctg gcagatcgaa
     3301 ctgattgaaa aacatcatag tcgtaaaaaa gatgcaccat ctggtactgc aaatatgctg
     3361 cttcaaacaa tccaagaagt gcaaaagctg cagcccgtat acaactggca agggaaaaaa
     3421 agagaagaaa accaaattgg tgtacacagt attcgtgcag gttctttacc tggtgaacat
     3481 gatgtattgt ttgctgctac tgatgaaata tttacgatca aacatgaatc tttttctaat
     3541 cgaatctttg ctgcaggcgc agtagaagca gcggactggt tgaaagatca gtcaccaggt
     3601 tattacaaat tagaagattt attaatggac aaaggggtgt aaaaaacgtg gaagctcaag
     3661 aaatcatccg ctacattcaa acggcatcta aaaagacgcc tgtgaaagtc tatctgaatg
     3721 taaccgagcc aatcgaattt aaagaaagta aagtttttgg tgaaggaagc agcttcgttg
     3781 tttttggtga ctatgagacg attgctccgg ttttagaagc tgaatcagaa aaaatcattg
     3841 aaatcgaaat cgaaactgct gcaagataca gcgcagttcc tttgctagat attaaaaaaa
     3901 ttaatgctcg tatcgaacca ggtgctatta tcagagatca agtgtcaatc ggcaacaatg
     3961 ccgtgatcat gatgggagcg attatcaata ttggtgcagt aatcggtgaa aacacgatga
     4021 ttgatatggg tgctgtctta ggtggacgag cgacagtggg caaaaactgc catattggtg
     4081 caggagctgt cttagcaggt gtgatcgaac cagcatcggc caaaccggtt atcgtagaag
     4141 acggcgtact ggtaggtgcg aatgcagtga ttgttgaagg tgtccatatc ggaaaagatg
     4201 cagtcgtggc agcaggagca gtggttttag aagatgtggc agcagaaact gttgtcggtg
     4261 gaatacctgc acgtgtatta aaaataagtg atgatcagac aaaagaaaat acagcattga
     4321 ttgctgcgtt acgcgaatta tagaggagat ggagaaaaat gcgcgaaata gatacctgta
     4381 ttgtgggggc aaccggatta gtcggccaaa cgatgctcaa agtgttagaa gaatatgatt
     4441 ttccagtagg acgattgaaa ttattagctt caaaacgttc agccggtaaa gtttgtgtat
     4501 tcaagaaaaa gaaacacact gtacaggaat tgacagaaac ttcatttgaa ggatatgatt
     4561 tagcgctatt ttctgcaggt gcttctgtat ccaaagagtt tgcgccaatc gcacgaagtc
     4621 agggactatt tgtcatagat aattcctcag catggcggga agattcggat attccattaa
     4681 tcgttccaga ggtcaatctg gaagactata cattgaatcg gctgattgca aatccaaatt
     4741 gttcaaccat tcaagcggta cttccgctta aagctttaca agaagccttt ggtatccagc
     4801 gagtcaatta ttcgacctat caagcagttt ctggttccgg gcaaaaaggc attgaagatc
     4861 ttcatcgtac gcaaaatgac gagaaaccag ttttgtatcc ttataatatt agccaaacgg
     4921 tgattccgga aatcgatcag cctttggaaa atgggtatac aaaagaagaa cagaaaatga
     4981 taaatgagac ccgtaagatt ctgcatcagc ctgctttgcc agtatcagca acctgcgtac
     5041 gtgtgccaat cgaaaatgga catggagtct ctgtggcagt tcaactgcaa aaagagttta
     5101 cgacggaaga aattagagag tgtctcagcc agtttccagg gattcaatta gaagatgatc
     5161 tagcagcaca taaatatccg acgagcattc ttgctagagg aacagatatg gtttatgttg
     5221 ggaggatcag aaaagacact tctttagaga acggattgtt gttctatacg actgccgata
     5281 acatacgcaa aggagccgct gcgaatgccg tacagattgc tgcagcttta atgcagaaaa
     5341 tagcggaggt ggcaagatga gtaatacgct gaaagtagcg aaattcggcg gaacatcgat
     5401 gtctcatgaa gagcaattca aaaaagtccg tacaattgtt ctttcagatc ccaatcgcca
     5461 agtgatcgtt gtttctgctt taggcaaacg tacgtcagat gatgacaaag taacagactt
     5521 actttatttg atttatgctt accttcagca tcatgttgat tggacacccc tttggaaaag
     5581 gatttgtgat cgtttcattc aagtaagaga tgttcttgcc ctgcagttac ctattgaaga
     5641 tgaactagca cgcatacaga aacaactgga agacgaaaag ataactgaag attatttagt
     5701 aagtagaggt gaatatctga ctgctcaact gatggctgag tatcttggtt accagtttat
     5761 tgacgcaaaa gacctgattt tctttgacta tgatgggaaa atcgatcagg aaaaaacagc
     5821 acaggaaatc acaaaagcat ttaaaagata tccgaaaatg gttgttccag ggttttacgg
     5881 ggcgaaccct gcaggaaaag aaaaattatt gggccgtggg ggaagcgaca ttaccggcgc
     5941 gatactggcg aagatgttgc aagcggaaag ttatgagaat tggacggatg tttcaggaat
     6001 catgatggct gatccgcgaa tcatcgatca tcctaaaatc atcgaggaaa tctcttttag
     6061 agaactgaga gagatggctt atatgggggc taacgttctt catgaagcag ctgttttccc
     6121 agttcaagag gctgatatcc ccatccaaat aaaaaatacc aatgatcctt tcgctcaagg
     6181 gacaaaaata tcgaatcacg aaatagaaaa ggaaaatgga ctgacaggaa tagctggacg
     6241 caaggatttt ctttccatca ctttattcaa acgtcatatg tccgatgaaa tcgggtttat
     6301 ctggaaagca atgagcattt ttgctaaaca tgagatatca atcgaacata ttccttcagg
     6361 gatcgacaat atcggagtga tcgtctcagc agaagcgatt gccgataagt tatttctcat
     6421 cacgaaagaa ttaaaagaaa cacttggcgt ggaagaaatc gtcgtgatcg aagacttagc
     6481 tttgatctct gtcgttgggg gtccgcataa agaattgatt ggattatctg ggaaagtgtt
     6541 atcgatattg aacaaattag aaattcgcac atcgatctta tcacaaggag cacaggagtt
     6601 gaatctgatc atcggtgtac cgaataatca atatgaaaca gtagtgaagg gtatctatga
     6661 agggatggtg aatacggatg ctgaaagaat atcgaatggc actccatcaa attcctgaaa
     6721 tcgggtttca tgaattcaag acaaaaaaat atttatatga acaagtaaaa aacagcgagg
     6781 ccgttatcca tgaaatcggc gaaacaggac tactccttta ttttgataat cagcaagaag
     6841 aaacgattgc ttttcgaaca gatatcgatg cactgccagt caaagaagaa acgaatctct
     6901 cttttgcttc taagcatgaa ggttatatgc atgcatgtgg tcatgacgga catatggcga
     6961 tgcttttagg gttgactgac tttataacta aacacattga ggatcttatt tataatgtag
     7021 tactgatcta tcagccatca gaagaaattt ccggtggcgc acaaagcgtt attcagtcag
     7081 gattgttgga taaatatgaa gtacaagcaa tatttggctt tcatctatgg cctggactag
     7141 ctaagggaga agttttctct cgaccaggac ctttgatggc acaaagcagt gaaacagaca
     7201 ttatcattaa aggccagtct gcacatattg cttctagtga aaaaggaatc gatagtctgg
     7261 aggcagctgt acgatttttg aagaatgttt atgattttga ccggcaatta ccggcagagt
     7321 ggaatcatct aatgaaattt ggccaaatcc aaggtggaac gatccgaaat gttttagctg
     7381 gagaagttat tgtatccgga agtatccgtt cttacagttc agatatgcaa aattatttga
     7441 agaggggatt agaccattta gcagaacagt ttcactctac agctactatt tcttttcgct
     7501 ataatgacgg ctatccagct gtaatcaacc atagaggctt atatgcagca ctgttagatt
     7561 caaacaaact acatgaatta gatgctccag ttcttcaagc cgaagatttt ggcatatata
     7621 cgcaacaata tccatgtgta tttttctttt taggtgtcgg tgatacccct gctttacatg
     7681 atccgacgtt cgattttgat atggatgttt tagaaaatgg tgtagaatgg tacaaaacga
     7741 ttctttttac agaaaatctt cttgtaaaaa tcgatagcag ttcaaaagag gagcgagtaa
     7801 ataatgatga atttatccaa tcgatttagt cctaggcttt ctagaataga agtatcaaaa
     7861 atacgacaat tcgatcagca aatatcgtcg ataccagatg tgatcaaact caccctggga
     7921 gaaccggatt ttccaactcc tgaacatgtc aagcaagcag gaatagcagc gattgaagaa
     7981 gacttttctc actataccgg aatgagagga ttagaggaac tgcgggaagc ggcttgtata
     8041 tttcagcaac agcgttatgg attaacttat gatccgcaaa cggaagtttt gacgacagtt
     8101 ggtgcaacgg aagcgattgc ctctgcgttg ttatctgttt tggaagaagg agacaaggtt
     8161 cttattccag cgcctgctta ttcaggatat cagccattag tggaactagc tggtgctgaa
     8221 ttgatcccga tagatacttc tgacacaggg tttgtctgtc agccggaaca atttgaacga
     8281 gcatttgaac aatatggatc agctgtcaaa gcagtcattt taaactatcc taacaatcct
     8341 actgggacga cattgtctgc aaaacaattg aaagcaatcg ctgaggtttt aaaaaaatat
     8401 ccggtatttg ttataagtga tgaagtatat gctgaattga cttatagtgg aacgcatatg
     8461 tccatagcta cttatcttcc agaacaaaca atcgtgataa gcgggttatc caagtcacat
     8521 gcgatgactg gttggcgagt gggctttatt tttgctcaaa aagcccttat tgacgaacta
     8581 attaaagtcc atcaatatct ggtgactgcc gctacaacga tgtcgcaaaa agctgcggct
     8641 gaagcgctaa taaatggggt gaaagatagc gagaatatga aagagcaata tcaaacacga
     8701 agagactatc tggtccagca attggcgcct ttaggatttg aagtgaccca gccaaacggt
     8761 gcattttact tgttttgcaa actcccagct gccattcaaa ctgactcttg gcagttttgt
     8821 gtagaattag cggaacaggc aaaagttgcg tgtatcccag gaattgcatt tggcccagaa
     8881 ggagaaggtt atatccggat ttcttatgct agcagcatgg aaaaattaca tgaggcctgc
     8941 aaacggatca aagacttttt aacagaacag tgattcatag attttatcaa aaaaacaatc
     9001 gttaattttg cggtatattc tgcaaggaga cgattgtttt ttaaatttag ttcaaatgaa
     9061 cgcgtttctt ttgattcata actaatgtaa tgttaagttt aaattgttta gaatgatttt
     9121 aaaagaatat tcaaggaggt ttcatcaatg gaaaaaatca aagttgtcat tgtaggtgca
     9181 tctcatggag ggcatcaatc gatcttggaa ctattgacac gatacggtga aaatgtagat
     9241 atcacgctat ttgaagcagg agactatatt tccttcatgt catgtggtat ggagctgtat
     9301 ttagaagaca aggtaacaga tgtaaatgat gtacgtaatt tcagaccaga aaactttccg
     9361 caatccaatg ttcatatttt aaataatcac gaagtaaaaa cgatcgatgc cgataaaaaa
     9421 acagtaacgg ttactagcgc atcagataac gaaacaaaag aatatcctta tgacaaattg
     9481 attttgagtt ctggtgtcaa accaaacaat ctgccagtac caggagcaga tctagaaaat
     9541 gtctacttga tgcgtggata caattgggca accaaaatca aagaaaaatt aaatgatccg
     9601 gctattaaaa aaatcgctgt cgtaggtgcg ggttacattg ggatcgaagc agcagaagca
     9661 agccgaaaag ctgggaaaga agtcgtattg cttgatgtga tcgatagacc tttaggcact
     9721 tatttagatg ctgagatgac agatatttta gaacaacacc taaatgaaaa cggtattgaa
     9781 gttttgacaa atgcaaaaat cgaagctttt actggtaacg gaaaagttga ggcaatcaaa
     9841 acaagtgaaa aagaaatccc agcagatcta gtcattcaag ctgccggtgt gaaggcaaat
     9901 acagaatggt taaaagggat cgtcgactta gatgaacgtg gctggatcca aacaaatgaa
     9961 tacttgcaaa cgaatcttcc agatgtgtat gcagtaggag atgctacact tgcttattct
    10021 atcccagcac gcaaaaaaat gccgatcgct ttagcgactg ttgcacgtag agaagcacgt
    10081 tatgtagtaa aacatctttt tgaagaagta ccaaacaaac cgttcggcgg agtcgtaggt
    10141 tcctctgcat taagtgtctt tgactatcat tttgcagcaa gcggattgaa tagctttaca
    10201 gcagcaaaag caggagtaga tgtacgagtg tcttattatg aagatacgtt acgaccaaaa
    10261 tatgtaccag aagaacaagg aaatccaaaa gtgtccatcc aattattctc ggatccaatg
    10321 acccatcaat tgctaggagg agcagtatta tctacctatg atgttacagg tcaaggaaat
    10381 gtattggcac tagcgatcca aaacaaactg actttagaag aactagcaga agctgacttc
    10441 ttcttccagc caggctttga ccgacaatgg agcatgttga atctagccgc tcaacaagcg
    10501 ttaggagaag aaccatttgt agaataagaa aacgaaatat ttgaaaagag gttgtgaaaa
    10561 agctgccttg catcaagtaa taagaacagc caagtaaaaa cagcttctta tgtttttaac
    10621 ttggctggac ttattaccgc agatgcaagc ttttgaacac cgtttatttt caaaaaaggg
    10681 actgtgacac gatttgtgta ccgaccccca aaagttagac caaaaaatct aacttttgga
    10741 ggtcggtatt ttttatggtt aaatatactt ttgaacttaa attaaagatc gttcatgatt
    10801 atttggatgg caaaggcgat tggaccgtaa aaatccagat caagagattg aagcggaaat
    10861 gcttaaaatt cgggagaagc acaaggatta tggctgttta cggatgacca atgaactaag
    10921 aaaccgcggt ttctctatca ataaaaagaa agttcagcgc ttgatcaaga agctaggaat
    10981 aaaagtaacg gcatacacgc gtaaatcacg tcgctacaat tcttatcgag gaaaagt
//
LOCUS       DACEXQ010000078        10878 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.078, whole genome shotgun sequence.
ACCESSION   DACEXQ010000078 DACEXQ010000000
VERSION     DACEXQ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.078"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..627
                     /locus_tag="IXH04_001433"
     CDS             <1..627
                     /locus_tag="IXH04_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ1852046.1"
                     /translation="NYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSYTV
                     EMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALDAY
                     SMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGKNG
                     FFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            829..1662
                     /locus_tag="IXH04_001434"
     CDS             829..1662
                     /locus_tag="IXH04_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAQ1852047.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            1678..2415
                     /locus_tag="IXH04_001435"
     CDS             1678..2415
                     /locus_tag="IXH04_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAQ1852048.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(2570..3538)
                     /locus_tag="IXH04_001436"
     CDS             complement(2570..3538)
                     /locus_tag="IXH04_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAQ1852049.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            3770..4603
                     /locus_tag="IXH04_001437"
     CDS             3770..4603
                     /locus_tag="IXH04_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAQ1852050.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            4637..5476
                     /locus_tag="IXH04_001438"
     CDS             4637..5476
                     /locus_tag="IXH04_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1852051.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            5502..6062
                     /locus_tag="IXH04_001439"
     CDS             5502..6062
                     /locus_tag="IXH04_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ1852052.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            6261..7982
                     /locus_tag="IXH04_001440"
     CDS             6261..7982
                     /locus_tag="IXH04_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAQ1852053.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            8147..9289
                     /locus_tag="IXH04_001441"
     CDS             8147..9289
                     /locus_tag="IXH04_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ1852054.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            9305..10516
                     /gene="thiI"
                     /locus_tag="IXH04_001442"
     CDS             9305..10516
                     /gene="thiI"
                     /locus_tag="IXH04_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAQ1852055.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         3713 a         1816 c         2295 g         3054 t
ORIGIN      
        1 aactatcatc ttgaccgagg actgacacca caaatttttt atgataaata catcggtgta
       61 aaattagatg attatgttag cgtgatcaat gcaccaacaa aagacaaacc attcaatcgt
      121 tcttatacag tagagatgct aggaaatgtc gttggcggga aagaagtcaa atatctgaat
      181 gttgatatgg aaactttcaa aaaattagcg attgctcagc ttgaagaagg agaatctgtt
      241 tggtttggtt gtgatgtagg tcaatcttct actagaacta gtggcatcat ggccttagat
      301 gcttattcaa tggatgatct atttgatacg gattttacaa tgactaaagc tgaacgttta
      361 gattttggag aaagtctgat gacccatgcg atggtattga ccggtgttga cttagtagac
      421 gggcaatcaa caaaatggaa agtcgaaaat agctggggcg aaaaagttgg aaagaatggc
      481 ttctttgtta tgagtgatgc ctggatggac gaatatacgt accaaatcgt tgtaagaaaa
      541 gatttattga cgaaagagca attaaatgcg ttcaatgaag aaccaattgt tctatctccg
      601 tgggatccaa tgggtgcttt agcatagacg agtatttcaa tgaagagtat gtagtaaaga
      661 taaaaagagc tggcttaagc cagctctttt tatctttaaa gtccattccc tgttttttat
      721 ggtccctgtg ctataattct aaataaagga taaggattgg aaataatcct tttatcatta
      781 aaaatatgat gacgaagtag aataaaattg ggagtagggg ggaaagtcat ggatatccaa
      841 actgttttat ctgatctgaa attaaatggt aaagtgattc cagttgttgg tggcgatgtt
      901 aatcagacct atcgtataaa aaccgaacat cgtgcttatt ttttaaaaat ccatccgaat
      961 gtaaaaaaag gattttttga agcagaagta gacggattaa aagaactgtc agcattcgta
     1021 cgtgtaccgg atacgtatat gctaggtgaa acaagtgaag gcgcttattt gttaatggaa
     1081 tggatcgagc cgggaaaagg ggatcaacga gatcttgctg cagctttagc taatctccat
     1141 cagcaaacag cgccacaatt tggttttcgt aaagataact atttgggtac acttgttcag
     1201 aaaaattctt ttgaagaaga ttggtggact tttttcttta aagatcggct tgaatcccag
     1261 atttctttgg cagaagaaac gaatcgatgg aatgtacagc gtcaagaaaa gtatctgcga
     1321 tttaaagaac gtgtattgaa atcggttgaa ccaaaaaaga tcactccacg cctcttgcac
     1381 ggagacctgt ggagtggtaa tgtgttcttt gaccagcaag ggcatccagt ttttgtcgac
     1441 ccagctgttt cttatgggaa tcgggaacaa gatattgcaa tgagccagtt attcggtggt
     1501 ttccgaccgg aatttttaga tgcttatcaa actatatttc cattagaaaa gggatggaaa
     1561 gaccgactgc cgatttacca gttatattat cttttggcac atttgaatat gtttggagaa
     1621 agttacggtt cccaagtaga tcaattacta gaaaattttt aagaggaggg cgtgtttttg
     1681 gcaaaaaaat ataccaaaaa gcatgaagtt tcatattatg agtgtgacat caaccaaacg
     1741 atgacatttc cttcgatgtt aggagtagtt atcaaaacgt ctgaagatca aagcgaccag
     1801 ttgggaagag gcagtgaatt cgtcaatagc tttggactta cttgggtcat cacgaattat
     1861 tccatggaga ttaatcgtct ccctagagtc ggtgaagtaa tctcagtaac gactcaagcc
     1921 atggagtaca ataagttttt ctgttatcgg aacttctgga tccatgatga agatgggaaa
     1981 gaattagtca aaatagaatc tgttttcgta ttgatggatt ttgtgaatcg aaaaatgagc
     2041 agtgttaatg aagagatcat tgctcctttt gagagtgaga agatcaagaa aatcaagcgt
     2101 caggaaaaaa ttgaaaagat tgattcgggc ttcatgttgc catatcgtgt tcgtttttat
     2161 gatatcgata gtaatcagca tgttaataat gcgatgtatt ttaattggat cattgacgta
     2221 ttaggatatg actttttgac tacgcatgtt ccagaaaaag taattattcg atttgataaa
     2281 gaagtagagt acgggaatga gatcgaaagc cattttgaac aaatccctat cgaaaatggt
     2341 gtcaaaacaa aacatgaaat ccgaatgcaa gaacagctgt attgtgaagc aaatattata
     2401 tggaaaaata aataaaaaag aggttgtgac agaagggggc aacttcaaga aataagaaga
     2461 aatttacgaa aattgtttct caaatttttg taaattttcg acttagtttt cgaagaagct
     2521 atttttgtca cagcctctct ttttaagttt agctattaga taatgatgtt tagttttgga
     2581 taaacgaaat caattcttca cccgacaaac catagtttac agcaatcaat agttttaaac
     2641 gtgctttttg actattaata ctgttacaga aaataatccc cattttttct agttcttttc
     2701 ccccgccttc ataatcataa acaggctctg cgattccatt gaaacaacga gaaacaagta
     2761 ctacagggat tttttggaca agaagctttt ccaaagcagc taaggcttgt ggagggagat
     2821 tccccgcacc taaagcttcg attaccaaac cgtctacttt cgtatgggcg atggcttcca
     2881 ataagtcccc ttgcatccca gcatatgctt taacgatcgg gatagtccca tctacagcag
     2941 aaatgtccag tcgctttgtt tctaaaagtt cctgcaagaa taaaattcga ttcttagtta
     3001 ctaagccgat gggacccaat gtaggtgttc ggaaagtagc aacatttgtg gtatgggtct
     3061 ttgtcacgta gcgtgcagaa tggatctcat cgttcatgac caccaaaacc cccttatcta
     3121 gcgcttcttc acaacttgcg acacgaatcg cactttcaaa attataaagg ccgtcacttc
     3181 caagttcatt acttgaacgc attgccccag tcaaaacaat tggtaatttg ttgccaatcg
     3241 ttgtatcaag aaaaaaagca gtttcttcta atgtatctgt accgtgtgtg atcaccactc
     3301 cggatatacc tgatgcataa gcggacaaga tacgttcttt taataccaac atgtgagaag
     3361 gcatgatgtg tggtgaggga agattgaaga tatcttcaac taataattct acatgcttgg
     3421 ggattgtgat ttctgcatct aataatgggt ttgccacatc aggactaaca ccgcccgtga
     3481 gcatgttttc tttcatggcg atcgttccgc cggtgtgtag tacaagtatt tttttcattt
     3541 tacagacttc cttacgaata attttgcata gccgttaaat cttattgtat actatataaa
     3601 agagttttgc tatcagtgaa gaataacgaa tagcctaact tatcataaaa aagctgacct
     3661 tattaaaatg gaaaagactc aaccttgacc tcctaataca atggagtatc tggttgagtt
     3721 tcttttacaa gaaagcagcc aaggaaatca atcacgaagg tggccggaaa tgatcagaat
     3781 cggattaaca tcgtttaaag aacatgacaa attaactggt aaaaagaata acactcttta
     3841 tgaatatgca gggtttctac ccttagtgga attagatact gcctattatg gaattccccg
     3901 aaagtcctca gttgagaatt ggataagtga agtacctgaa tcatttcgct ttgtagtcaa
     3961 agtctatcaa ggaatcagcg gtcaaggaga ctggaagcct tattatcaga ccgaagaaga
     4021 gatgattgcg cattttttag atgttatgtc gccaatggta gatgctggaa agatgttttg
     4081 ctttttagtc caattcccct catcattcaa atgtacgaaa gaaaatgtcc agtacttgcg
     4141 caaacttaga aagtggtttg agggttatcc tgtttctata gaattgcggg attattcttg
     4201 gtacagcgat gaatttcttg aacgtacaag aaactttatg tctagagccg ctttttcttt
     4261 agccatcatc gatgagccac agttgctgaa tacaacgatt ccttttgatc catttgtcac
     4321 caatccgaac tttgctcttt ttcgattcca tggtaggaat gcgcaaggat ggcaagcaaa
     4381 tgataaagag tggcgtaaaa aaagaacgtt atatcgttat tcagaaaaag aactgcaaga
     4441 actagcacat gaagtcaaag tagttgattc taagacaaaa gaggtaggtg tgatttttaa
     4501 taataattct ggtggtgatg cggctgaaaa tgcattagca ctgcaaaaaa tattaaatat
     4561 caagccagat ggactcaatc caacccaaat ggatctgttt taagggctta ttttattcaa
     4621 gaaaggggcg aagtctatga tcaaagctat tttttttgat attgacggaa cattggtcac
     4681 caatcgttca aaagcgttgg aatcaacaaa acaagcaatt gaatatgctc ggaaaaacgg
     4741 cattttatgc ggtgtagcaa cggggcgcag cccagtaaag atcaaagaaa tcattgatga
     4801 attggaatta gatatgtatg tcgtgtataa tggtcaactg gtttttactg cagatcgcac
     4861 gattatcgac cacccttttg aacaaaaagt attggaacat attgttgaat ttgcagatga
     4921 gaaccatcga caaatcgttt ttggtgcgag aaatcggtta gatggatcaa cgacgatgct
     4981 attaggacaa tcgattttta tcaagcgact agtcagcttt ttaccgcgta aattcccggt
     5041 acgtctgatg aaaaaaatcc tccaggtttt tagccctcac cgacaaaagg atcgttatga
     5101 aacgttagag atattaaaag aaccaattta tcaatgtata ttgctcagtc cggagtcaga
     5161 acagaaaaag ttagaacatc gctttcccga gtgtacgttt cagcgttcaa atacgtatac
     5221 tgtagatatc atcccaaaag gtggttcaaa attactaggc atccaagctt tcgcaaatgc
     5281 tgtcgaaatt gaaatggaag aaattatggc attcggcgat cattacaatg atatcgagat
     5341 gctgaaaggt gtaggaattg gagtggccat ggggaatgct cagatcgaag taaaacatgc
     5401 tgcagacttt gtgactcaat caaatgaaga agatgggata tacgaaggac tgaaacactt
     5461 cggtctgatt tattagtcaa tgaaagaagg aaaaaatcag tatggaagca gagacacctt
     5521 ttcaaaaagt agaaacattc cacaaaactt ttgatccaag aagaccagag atacccacgg
     5581 cttttacaga aaatgaagct catacacgtg ctgcgttcaa aatagaagaa cttgttgaat
     5641 ttgttgcagc tacttcacct aataaagaac aactgcatga agctgttatg ctgctgcacg
     5701 aagcattaga taaagcagaa gaaaaaatac ttggtaaaac acagtgggga agtgatccgc
     5761 tggttgaaca agtagatgca ctgacagata tcctttactt tacttatggt tctttttcat
     5821 tactaggaat agtgcctgat cgaatattcg aaatcgtcca tcaggcaaat atggggaaat
     5881 tatttccaga tggaaaacct cattatgacc ctgtaacaaa taaagtgttg aaacctgaaa
     5941 actggcagat ggaatatgct ccagaaccta aaatcgcggc agaaatccaa cgtcagattc
     6001 aagaagcgat tgctaagaag aaatccgctg atataggcat gtgcacaaat gaaaaagagt
     6061 gatcagagtt tcactatttt tgttatcttt atacgatgga aaaacaaaag attttcgttt
     6121 aagttagtga atgcatgaaa atagtatgct ataatctaga atagatgtct tttgcacgag
     6181 agacgtatta tttggattta tgccaagcag aacgccatct gttgcataaa agatgcatac
     6241 tgaaaacgga ggaaccataa atgggaaata atgttatcat cggaatcgtc attgcaatca
     6301 ttatagtcgc cattgtcgtc tttttggtta gttacttgat gaagaaaaaa aaccaagatc
     6361 gattggatgc cttagaaaaa cgcaaggaag aattatttga cttgccagtg attgaagaag
     6421 tcgatgatgt taaaaaaatg catctggttg ggcaaagcca aaataccttt cgtgaatgga
     6481 gtcaaaaatg gaatgacata tctacttcct cgttcgctga actagaaagt caaattttcg
     6541 aagtggaaaa cctcaatgaa agttttcggt tcttaaaagg aaaaaaagca gttgttgaag
     6601 cagaagctac aatggaccaa atggagaaac aagtgacgga aatccgtgaa ggtctaaagg
     6661 aattgcggga aacggaagaa cgcaactcac tagctgttca gcaggcactt gacgtctatg
     6721 aagaactgaa agaaaaagtc agagaagacg agcatgaata cggaccagct ctttttgaat
     6781 tgcaaaaaca gatcaaagtg atcgaaaaag aatttacgca gtttgtgact ttaaacactt
     6841 ctggagaccc tgttgaagcc cgcgaggttt tggataaagc agaaaaacat acgtatgaag
     6901 tggaagctct gatgaaaaaa attccaccgc tgtatgagga gctccatacc actttccctg
     6961 aacaacttga agaaatcagt gatacttacg agaaattgat ggaagaacaa tatgtattcc
     7021 cagaagaaga tcttgctgaa gatatcgcaa aagtaagtcg aagaattgaa aattcattag
     7081 ccaatcttga aaaaacagaa gttgaaacgg tcgaatttga aaaccgtgaa acagctgatt
     7141 tgattgattc gttatatgat atcttagaac gggaaatgga agctcagaga tatgtaaaaa
     7201 ctaaccaatc aacgattgca gagtatatca aacatacaac aaagaataac cgtcaattat
     7261 tgattgagct ggatcacaca tctcaaagct atacattgaa ccacaatgaa attggccgtg
     7321 tacgaggttt ccaaaccgaa gtggaagaaa tggaacgtca aaatgaacag atgattccac
     7381 agatccgtca acacgaaatt ccttattcag aagtgcgcac gttctataaa acagtcttca
     7441 aagtcttaga agatatcgaa aagcaacaag tagagatcga cgactcatta catgaattga
     7501 gaaaaggcga aaaagaagcg caagaaaaga tcgatacatt cgaattcaaa ttgcgcagtt
     7561 tgaaacgttt tgtagaaaaa caacgtttgc ctggcttgcc gaatgattac ctagaattct
     7621 tttttgttgc tactgaccgt attgaagaac taagtgttgt cttgaataaa attcgagtaa
     7681 acatggaaga ggtcaatcgt ttagttgctc tatgtgaaga agatctagaa ttattagata
     7741 agaagacaca tgatttagta gatgcagcag cattaacgga gcaaatgctc caatatgcaa
     7801 atcgttatcg tcatacacac gaagaagtga gagaagcaat tgataaaagc tactatttat
     7861 tcaataaaga atatcactat caagaagcat tagacgaaat cggtacagca ttagagcgag
     7921 tagaacctgg tgctttcaaa cgcatcgaag atttttattt taatcatcca gacttagtat
     7981 aaagcaaaaa agttagtaaa ccagcaagag caagacggat tattgtcttg ttcttgtctt
     8041 tttttgtata tttgaaaatt gaaacaagtg aaaatttcac ctgctcttga aagcaccttg
     8101 gacaaccgtt ataatagaaa agaatttcaa aaaagagggg tttcttatga tatattttga
     8161 taacagtgca acaaccccaa tctttccgca aagcttagat gcatatgtaa aaaccagtca
     8221 gcggattatt gggaatccat caagcttgca tgatttaggc aatcaagcta accggttgtt
     8281 gcagcaagca agaaagcaga tcgctgattt gatccatgta gaaccagaag aaatctattt
     8341 tacttcaggc ggaacagaag gagataactg ggtattaaaa ggaacgatga tcgaaaaaag
     8401 agaatatggg aatcatatga tcatttcagg agtcgaacat ccggccgtca gcgagacagc
     8461 agaacaatta aaagaattag gaatcgaagt aagtatcgcc ccagtggata aacgcggatt
     8521 tgtacgtgtc gacgaattga aagaattaat aagaaaagag acagtattag tctctgttat
     8581 ggctgtgaat aatgaagtag gatcgattca gcccatccag gaaatcagtg atcttttgga
     8641 tgattttcct aaaattcatt tccatgtaga tgctgttcag gcgattggaa aagtaccatt
     8701 ctcgcaatgg ttgacagaaa gagttgattt tgcaacattt tcagctcata aattccatgg
     8761 cccacgggga actggtttca tttattggaa aaagggtcgt cgtttagcac cattactgac
     8821 aggtggaggg caagaaagaa atcagcgaag tgggacagaa aatgttccag gtattgtggc
     8881 tatggttaaa gctttacgtt tatcattaga gataaaggaa caaaaacctg agcagacaca
     8941 acgcttaaaa gtagctctaa tcaatgcgtt gaagacgtat gagaaagtga cgattttttc
     9001 agaaggaact gactatgcac cgcatatttt atgttttgct ctaaaaggaa ttcgcggcga
     9061 agttctcgtc catgccttag aagaaaaaca aatatttgtt tctactacta gtgcctgctc
     9121 ctcacgtaaa catatggcaa gcagtacatt gcacgcaatg catgtaccaa atgatctggc
     9181 tacatctgct gttcgaatca gcttagatga aaacaattcg cttgcagaag tcgagcaatt
     9241 tatgattatt ttcaagcagc tttataagaa attttctgca atcaattaaa agaggagtgt
     9301 tcttgtgaaa tacagtgaaa ttatggtccg ttatggggaa ctttcgacaa aaggaaagaa
     9361 tcgaaaaagc tttatcatgc aactagcaca aaatgtcaga caagctttga aagactttcc
     9421 agaaatcaaa atccatgctg atcgggaccg gatgcatctt ttattgaatg gcgcagacgg
     9481 gcagctagtt attccaaaac tagcaaaaat atttgggatc caaaatttct ctccaagtat
     9541 tcgtgtagaa aaagatgtcg aagttttgaa acaagctgtt caagacatca tgaaagaaat
     9601 ttatactggg aaagaaacat ttaaaattat cgctaaaaga agcgatcacc aatttgaatt
     9661 gacaagtaat gaattgaatc aaacattagg taatgctgtt tttgatattt tcccgcatat
     9721 ccaagttcaa atgcgtcaac cagatattcc attacgtgta gagattcggc gtgatggcgc
     9781 ttatctatcg tatgaaacga tcaagggagc tggaggattg cccgtaggaa caagcggaaa
     9841 aggaatgctt atgctttctg gggggattga ttctcctgta gcagggtact ttgccatgaa
     9901 gcggggagtg gaaattgaag ctgttcattt tgctagtccg ccgtatacaa gtgaacaagc
     9961 actgcaaaaa gcaaaagatc taacagccaa actagcacct tatgtgggaa cgatccattt
    10021 tatcgaagtg ccatttaccg agattcaaga agaaatcaaa aagtcagtgc cacaaggata
    10081 ttggatgaca attactcgtc gaatgatgtt gcgtttgacc gatgaaattc gccgtattcg
    10141 tcatggttta gtcattttga atggtgaatc attaggtcaa gtggcttcac aaactttgca
    10201 aagtatggtg gctatcaatg aagtaacgaa tacgccaatc attcggcctg tagcgacaat
    10261 ggataaactt gaaatcattg aagtagcaga acagatcgat acatttaatc tttctatcca
    10321 gccttttgaa gattgctgta cgatttttgc tcctccacag ccaaaaacac gccctcaact
    10381 ggaaaaggtc catcaatatg aagagcgttt agcaatagat gaaatgatcc aacgagcact
    10441 agctggcttg aagatggaag agatacaagc agaatcgtct aatgaaaaaa ctgaagaatt
    10501 ttcagaattg ctataagagg ttatgaaaat agtgttttga ccagagtatt agagaaacaa
    10561 aacgaaaaaa tagctccatg tattttaccg tttttgaact aatgtcgaag attagctgat
    10621 tgaataccat ttatcaagaa gttatggaaa agccggttct tttttactaa ctgaaaaata
    10681 aaattttatt tagagatcaa gctacagttg ataaatgata taaaaaagag aatttcccat
    10741 gaaatagcga ggttgtgaca gaagtgggca gcttcaagaa ataagaagga atttacgaaa
    10801 attgcttttc aaatttttgt gaaatttcgg cttatttttc gaagaagtta cttctggaac
    10861 accgtgtatc agaggttg
//
LOCUS       DACEXQ010000079        10754 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.079, whole genome shotgun sequence.
ACCESSION   DACEXQ010000079 DACEXQ010000000
VERSION     DACEXQ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.079"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(228..554)
                     /locus_tag="IXH04_001443"
     CDS             complement(228..554)
                     /locus_tag="IXH04_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852056.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(927..2128)
                     /locus_tag="IXH04_001444"
                     /pseudo
     CDS             complement(927..2128)
                     /locus_tag="IXH04_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            2245..2760
                     /locus_tag="IXH04_001445"
     CDS             2245..2760
                     /locus_tag="IXH04_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1852057.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(2959..3405)
                     /locus_tag="IXH04_001446"
     CDS             complement(2959..3405)
                     /locus_tag="IXH04_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAQ1852058.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(3452..4930)
                     /gene="msr"
                     /locus_tag="IXH04_001447"
     CDS             complement(3452..4930)
                     /gene="msr"
                     /locus_tag="IXH04_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ1852059.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(5284..5913)
                     /locus_tag="IXH04_001448"
     CDS             complement(5284..5913)
                     /locus_tag="IXH04_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ1852060.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(5897..6361)
                     /locus_tag="IXH04_001449"
     CDS             complement(5897..6361)
                     /locus_tag="IXH04_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1852061.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(6848..8116)
                     /locus_tag="IXH04_001450"
     CDS             complement(6848..8116)
                     /locus_tag="IXH04_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAQ1852062.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(8347..9084)
                     /locus_tag="IXH04_001451"
     CDS             complement(8347..9084)
                     /locus_tag="IXH04_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1852063.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(9151..9720)
                     /locus_tag="IXH04_001452"
     CDS             complement(9151..9720)
                     /locus_tag="IXH04_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAQ1852064.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            9848..10720
                     /locus_tag="IXH04_001453"
     CDS             9848..10720
                     /locus_tag="IXH04_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1852065.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
BASE COUNT         3188 a         2257 c         1879 g         3430 t
ORIGIN      
        1 tttttcgatc attcaatcgc ttatacgatt acgctactga ttgctgggat ttttcactta
       61 tcttataaat atcagctgaa taaaaaccac ttgaatcgga ctttccgcta aaaaatatcc
      121 gtacgataag tagagtgcct tgaagatcgg caaaaaactg ccggactttt tagaggctgt
      181 gacaaagatt ttgtcacagc ctctttttcc atataaacgg cgttcgccta ccagctgcct
      241 ggtaaatctg tttcctcttc gttaagaagc gaaaatccgt caaatgtttc gccatcataa
      301 taacgaagca gctcatccat ggacaagttc atcgtttttt ctttttcttc ttccgctgat
      361 tcagccagta gctgttcccg atacacctga tacataccag ctattttttg ctgcatcgct
      421 tttctagttt caccttccgc attgattatt ggcaattctt tgatatagcc acgataaatc
      481 aaccgatcat ctgaaaattc aatgggtaat tcaacgagtg tcaacccgac ttcctgaaaa
      541 tattcttcca gcatctatta tccccccatt ctttttctta aatgaatttt agcttaccta
      601 ctgaaaattt tccagtttca ttattttgag aaaaaaaggg aatgtgacaa aaatcgtgtc
      661 acatcccctt tttccgaata aatggtgttc ttctgccaac tcctcaatca taagtcacaa
      721 tattcaacaa acatgagaaa ctgttttttg aaattgttcc ttgtgattca gagttaaacg
      781 acagtctcac aacctcttgg taaacggcgt tcaaaagctt gcatctgcgg taataagccc
      841 aaccaaacga aaaatatgag aagctatttt tgtttggctg ttcttatgac ttgatgcagg
      901 acagcttttt cacaacctct gatttcttat tcttcttcat ttgtagcaaa ataattacgg
      961 tgataaatga tacaacatac actgataaga acaatggcac ctgcaaaata aaacgcatag
     1021 ctttcaccaa tatgttcacc aatcacacct gcaataaatg gtgctaaagc tccaccgaaa
     1081 aagcggacaa aactatagct ggacgaagca acgtttcgtt gcagacctgg tatttccata
     1141 gcaatcgtcg tcaacagtgt attgataatt ccttggaaaa atcctgctaa cacaatgctg
     1201 atcggtacaa ggactggcat agaagttcca gctcctaata acagcaaaca aataatgaat
     1261 aagacaaatg ctgctaaaat tgtccgataa gtcgtaaact tccgctcaag agccggtgca
     1321 acgaaaatcg aagcaatagc cagaagcaca ccccagccaa aaaatacaaa accgacttct
     1381 aattcagaat agccaatcaa taaaaatggc gcatacgcaa gtaaagtaaa gaatccgaaa
     1441 ttatataaca atgcgattag tccaactgaa cgaagtttat gattgcttaa cgctttgaca
     1501 ccttcccaaa ggcctacttt tctctttggt ttctcagttt caggtaaaag taccgctagg
     1561 gctataaaac cgcaaaacat caaaaatgct accccaaaaa atggataacg ccatgaacca
     1621 ctgcccaaga ccccaccgac tagagggcca acagccattc cgcagccaag tgctgcttca
     1681 tacatcatga ttgctttttc tgtgttacca actaaaatac tgacgatcgt tgctaaagca
     1741 gtagagacaa ataaagcatt cccgacaccc cagccggcac gtaacccaat cagcattccg
     1801 atattcccag ataatccgcc aagaccagcg aatacaacga tgataaacaa tcctgtcatc
     1861 aatgtttttt caaaccgatc cgactagata agaaacctgt aaatagcatc accacaccag
     1921 tgactaacat gtaactagta aataacaggg tcgtttcagc tggcgtagcg tttaactgtg
     1981 cagcaatcgt ttttaagatt gggtctacta gacccactcc cataaaagcg atcatacagg
     2041 tagctgctac aactagcgct gaacttttcc cctttgtttc ttcatttgtt tgttcttgta
     2101 tatctatttg attctccaat atttccatcc ctttctctct attagcatca tacatgattt
     2161 attttacatg taaatatgca catgtaaaac gtggaaactt tactgtgata taataaagaa
     2221 aataagacga gaggtaggat ttttatgaat actttagcct atatattatt atgcgtgctt
     2281 ttatccaaac cacgttctgg gtatgaattg aagcagctga tcacaatttt ttgggaagcc
     2341 catcacagtc agatctatac aacgcttgcc aagttagacc agcagggtta tgtgaccgtt
     2401 ttggacaaag acgagcactc tcaaaaaaag atctaccact tgactgatga aggacgtgct
     2461 cttgtagagg aatggatcaa agaggaaact cccgctccta cgcaaaaaga tgaatttcta
     2521 gcaaaaatct atgctttttc gacgctcgat aaaacaacag ctactggact gctattcact
     2581 agagaacaac agctgaacaa aatcatgcaa aaaaatcaaa aaaaattgaa tgacctctcg
     2641 ctagacaaga aacaagaatt tggacgttat gttgtgatcc agcgcagaat tatgctttgt
     2701 cagcaggaac tccaatggtg tcagcaagta agagaacaaa tgcagcagtt ttttgagtaa
     2761 gggataaaca gaacctttct agaaatatct attgaagagc aaattatctt caataaaaga
     2821 gcaatacagt tggaaaagaa aaagcgatcc gaacgacagg ccaaaaccta tcgttcggat
     2881 cactattttt ctagaactgc attcaagaga ctatgacaaa aagcatgcca aggccttttt
     2941 tcaaataaca gtgttccatc attcattact ttgaattgct tttagcacac ttcttgaaag
     3001 cgggccaacg atgatcaact gcaacggtaa agcggcaatc acattcattt tccaagcatt
     3061 catataagca gagaataatt gatcaggtat tccttgttcc atgatcacac cgaataaaga
     3121 catacatgtc accattccta aaaccatcaa gcatgaaata gttaagatca tttggacctt
     3181 gctttctgga tttactggta gtttaaaggc aatttttttc gccagcacac cgatgatgaa
     3241 gacatccaaa atgaaagcca ccacgaatcc aggcactaga ccaatcaaca aacttgataa
     3301 atgaagatct tgatgcagcc ataaattata cgcgctcatc cccaagacca tcaaaaaaca
     3361 catcattgtt gtaaaaatta ttccttcttt tttatttgta ggcaatctgt accactcctt
     3421 taaatttgtc acgaaaatca tcgtagcaca tttaaaaatt tctcgtaagt acttttttta
     3481 ttttctgccc ttttatttca taaacttcat cagccgtacg ctcgacaaag taagaatcat
     3541 gagaagtaaa caaaatcgtt cccggatata tttgcattag cttctctaaa gcttcgatcg
     3601 ttgccatatc aataaaatta gtcggttcat ctaacagcaa cacattactt ggcttagtaa
     3661 aaagcaaagc aatcgtcaga cgggtcgctt ctcccccact taatgtacat aacggtttcg
     3721 tcaaagtttc ctgagcaaac cctaagtgat tcagcaaact acggacaagc gattctgaaa
     3781 actctgtttg tctcatgaca aaacgaatga tggattcttc agatgtcatt tgataaccct
     3841 tttgctgata tacttgaaag cttactttcg gagagagaag gattccttct ccttgtttgc
     3901 ggatatgttc taataaggtt gtcttacctg aaccattttc accgacaatc gcgatttttt
     3961 tacctaaaga aaactgaaaa tcaccattta ccagtaatgt tctcccactt ctttccaatt
     4021 ggacattttg tcccatgatg ggatagcggc tgtggagttc aagagatttt ggtactggaa
     4081 aacggatttg tttcatctgc tgcggcttgg ttgtttctcc tattttttgg agtcttctct
     4141 ctaatgtttt cgcattcttt tggatggctt tttgtacact gtctttttgt ttagaggaag
     4201 acaaccgatc cggtctgatt tgttgctttt tctttttact ggacactttt tccaactttt
     4261 ccgcttgttt tttcttttct tggatcgatt tttccaatcg ttttttctct ttcatgatcg
     4321 actctgcttc tcgtagctga gtctttttct ccaattcctt ttgagtgaga taggcactat
     4381 aattccccga aaactctcgg atttctccgt cttttacttc ccagattttc gatgctagct
     4441 catctagaaa atagcgatca tgactaacaa aaaccagcgt accataataa taacgaagct
     4501 cttcaatcag tcgatcaata ctttttctat ctagatgggt tgttggctcg tctagaagca
     4561 tcccttgttc ataagaagaa agaagacgta tcaactcgat ctttcgttct tcccctccgc
     4621 tccatttccg ctctgaagtt tcttccagtc ttaactggaa atgactggct aattccaaat
     4681 ttaggtcctt tgcttcagcg agataggtta attgaggcag gtagttgaag gtgatctctg
     4741 tttggatttt cccatgatcg ggaaaaagtt caccggctat tagtttgagc aaagtcgatt
     4801 taccgacacc attttctcct atgatcccta tacgatcttg ctgatagaca cgaagagaat
     4861 caatagatag ttctagctgg ttgccaaagc tgacttgtag atttgttata tttactgcta
     4921 aattttccat atgttttaca ctcctatttc atgtaaaaca acaattatcg atactaaaaa
     4981 agagcagaaa aatctgtttg caaaaaaact gcatcacatc ttttcctact ctgaacgaaa
     5041 taactatgac taaatcacca aatctgtcat agctttcacc tattctactg tcgttcgaaa
     5101 aaatggacat actccatcca tgaaattgtt gtttatacat gtcttttgaa tttatttagt
     5161 tgttattcaa aagttcgaaa cgtaatttca tcgatgcagt catttttccg acctcctttt
     5221 ttattaggtt accacacacg attcaaaaaa agcaatagct actgaatagt aactgaaaac
     5281 cttttattcg aacgtagtga tcttttctgg tgctaaacga acagactcgt aaccttcttc
     5341 taccgctttt cctattgata aaataataac tggaacataa cggtctttat ccaaaccaaa
     5401 tgcctctgcc aactgatctg cttcgaatcc accaattgga tttgtttcat agccatgatc
     5461 acgcgcaact aacatgaatt gcatagccgc taagctagaa tcgactttga ctacatcgtt
     5521 catttcttct cttgaaaaac tcttgtaata aggaatgatc gcagctaatt gttgatctct
     5581 gacttctttt ggcatcttcc ctgattcgta agcttggtta tagatttctt cgccatattc
     5641 gtaacattgc atatcgccaa aaatcatgac cattgcagaa gaagtatcat tttgtctcgt
     5701 attgaaacgg atcaaaggac gcaagatttc ttttgcttcc tcgctttcag cgaccacaaa
     5761 gcgccatggt tgcatattga cagaagaagg tgcagtcgtc gcttctttga tcatttccag
     5821 catttcttcg tgtgggattt tgtatgtttc atcatatacg cgaacggatc ttctaccagt
     5881 cataattgtt gaaaaatcat ttttttgttc cattattatt aacctctttc gtttctgttg
     5941 ttatattgca aagttgggta ttcaatttat ctaaaagtgt tagaaattgt ttcatttcta
     6001 catcgctgaa agccggggat agcacggaac ctgtatcttt atggtccatt tcacaatgct
     6061 cgattttctt ctgggctttt tctgttagcg ctacatacac ttcccggttg ttctgaggat
     6121 ttctctgacg cgaaacatag ccatttgctt ccaatacctt cagatgacga gtaactgctc
     6181 cttgatcaat ctgcaacttt tcttgcaaac tgctttgtag caatggattt tcttctttca
     6241 gtctcatcat gatctcgtat ctagtaagac ttataccgat attttcttca aagatctgga
     6301 cgattttttg atcggctaat ttgatttgat ataaaaggtg actaatctct tctaaagaca
     6361 taatttcctc ctttctaata attgactcgt caacgattga cttgtcaata atagaataaa
     6421 aaaagaaaaa agtcaaagaa tcagctgtgt aataaaaaaa acgctctcaa tagaaaagta
     6481 tagagaaaag aaattttttg tagaatgaac ggaaaaaatg ataaagaaag cacaacgcta
     6541 aaaccaagat tattgattct tatcaaacta tagaagatag ctcttctgca gtagaaaatt
     6601 ttacaccgcc aaaaatttcc gaaatcgcct tttgcaagaa tcccgtcgaa tattagaaat
     6661 tggatcagta tgcttaaaca gtaataaaaa agttatgaca agatgttgtc acaacctttt
     6721 ttgagtaaac ggtgttcaaa aacttgcatc tgcggtaata agttcaacca aacaaaaaac
     6781 atgaaaatct gtttttgttt ggctgttctt actacttgat gcagggcagc tttttcacta
     6841 cctcttttta ataaccataa aatcctattc cttcatatct ccagcccatc ggtgttagcc
     6901 aatctctttc cttagcactc aaggtatagt gatgggtacc agttttttca tttggattaa
     6961 atagtcgata aattggcttg gaaccaccag aataaaaact tagaccttca taacgccaac
     7021 cgatttttgt taaagagttg atctcagatt gtgccattgt gtaatgatgg tctccactgt
     7081 aaggattgta taaacggtag acaggttgtc cgctgtttgg tgcttgccaa gcaatccctt
     7141 catattccca gcctacagtt ggcagataat ttttttctcc ggcattcaat gtatataaat
     7201 gttctcctgt atgacggtta tacagacgat acatcggaga agttgaagtt ggtgctgtat
     7261 catactgtgt cagattatag gtttcgatta cattgttcaa tgtagaacca taattaggcg
     7321 cagtggcata acgacctgtt agccaagccg tagcatcttt gtaagagttc gtattgcttt
     7381 tccatgcacc ggaatagtag taaacgcctg gctgaaaaga agtcgtcttc aataccgtta
     7441 cgttatcttg caatgattct ttgtacgaag gatattttct aaaagcggca ttcaccgtaa
     7501 tccattgccc attcacatat tccgaagtag gcatcgtaac agattggcca ttatagcttc
     7561 ctttaatccc aaatagattg taattagggg cagaagccaa agtactgttc ccccagccac
     7621 tttcaacgat ggcttgagca atcatgactg atgcatataa gtcattggca cttgccaaac
     7681 tttgtgcagt tgaggcaata ctatcaatga acgcttgttg agaattagca gatgagcgag
     7741 cataaaaagc atagccagta tagttttcga attcagacaa atctgcttga tgggtatgtg
     7801 tcaatgtttg cccatctgtc aattggttcg tgatttcttc tgcattctct ggattgttct
     7861 gagcttctgc tgatgtactg tcttcggttt gtgtttgttc tgttgtttca gttgttggtt
     7921 cttcggttga aattgtattt tctgagacag tagctgactg caaagtactc tctgtatcgt
     7981 tttcaattcc tgtttctgaa gaactcgatg tctgagttgc gacagtttct gtcgattctt
     8041 cagctagcac tgttgtgttg gcaactgcta gatttcctaa aagaataacg ctaaataata
     8101 aattctttgc tttcatcttt tttctccttt gaattatgaa acttttccta aataacagtg
     8161 taactaaaaa agtaaaagca agtcaatgtc catatttaaa taacatttgt ttctttttga
     8221 taaatttgct ttttctacat aaaaagacgt aaatgaagaa tattcttctc cattcacgcc
     8281 ttaaattgat ggtcgtttga ataactattt aacaagtaat tctctttgga tattatccgt
     8341 tatggattat acttcctgtg tcgtcggacg gataatcatg ttactgacag acatccggtc
     8401 tggcgtttct atcgcaaatg ctacagcaga agctatatct tcggaggtca atccccattc
     8461 tttttgcgct tcatgaagtt caagagcagc ctttttgtcg ctgatggttt gatataattc
     8521 agtttgaaca gctcctggag agatgatcgt agatttgatg ttattttgcc gttgctcttg
     8581 acgtaatcct tccatgattg cacggacagc aaattttgtt ccgcaataaa cggcggaatc
     8641 aggataaaca acatgtcccg ccacagaatc agttgaaatg atatgcccag atttttgttt
     8701 ttccatgatt ggaagtacgg cggaaattcc atttaacaca cccatgatgt tgatatcaag
     8761 catattttgc cattcatcac ggtgaccttc aactaatgga gcggttggca tgattcctgc
     8821 attattgtac aatacatcga ttcgaccgaa cttttccatc gctaaatcga taactgcttg
     8881 gacttgtgca aaatctctta catctgctgt ttgaatatat agctcagctt ctggtaaaga
     8941 ttcttttatt gcttttaggc gatcttctcg gcgtgcagct ataactagtt tagccccttt
     9001 ttctgctaat agcttcgttg ttgcttcacc gattccgctt gaagcgccca tgatgacaat
     9061 cactttttct gataaatcat tcattctatt ctcctcctgt tttgaaaaat ctcttgctta
     9121 tactcactat ctcttatttc atcttactgt tcaaataatc cgttcgacag gtttgacgat
     9181 tttggcaggg attcctgcaa cgatggtatt gtctgggaca tctttcgtaa cagttgcatt
     9241 cgctgcaaca atggcattct cgccaatcgt tacgcctggc agaatagttg ctcctgctcc
     9301 gatccaagca tttttcttga tgtggattgg ctttactgac aagcctcttc gttcttttgg
     9361 atcggttaga tggttcaccg tgatgaggtt gacacgagga ccaatcaata ctgaatcttc
     9421 taaacagatg ccacctaagt caacgaacat cacttgacga ttgataaaga catttttccc
     9481 tatactaata tgtttgccga aatctgtata aaaaggcagc agaacagtca ccgtatcatc
     9541 tactttgtca ctaatgatat tttctagccg tttgcggata tctgctggtt catggtaccc
     9601 agtattcagc gctgctaaag gctcttcgtt ttcagcttgg atctgatgga tcacttcaaa
     9661 gagcggatcc gttactcgaa tctctttatt gacgatttgt ttttcgatga ggcttgtcat
     9721 tttgctccct ccttttatcc ttactttata acggacggct tatcatgtct aatacttata
     9781 aaacatactt aactattcct tttaactata cttagtggta tgctttttta aaaacaggag
     9841 ggaaaaaatg gatattcgta tactgacgta ttttttagca gtagctagag aaaaaaatat
     9901 cagccgagca gcaaaatctc tgcatatcac gcaacctact ctctcaaaac aactgaaaga
     9961 tttagaagaa gaacttggtg tgacactttt cactcggggc tcccgagaga tacagttgac
    10021 ggattctggt cgatatcttt attcaaaagg aaaagaaatc ttgtcgttgg tagaaaaaac
    10081 ggcgaacaat ttaacaaatg acgcaatcat ctctggagaa atctacattg gcggaggcga
    10141 aactaaagca atctctttca tttctgatag ccttcattcg ttgatcaagg cgcatcctga
    10201 tatccagttt catttatata gcgggaatgc agatgatatc atagacaaac tggatagcgg
    10261 attacttgat tttggactcg taatcgaacc gacaaacaaa caaaaatacc agtcatttcg
    10321 actgccagcc caagatcgtt ggggactttt gatacatcga aatcatcctt tagcaaagca
    10381 aaccgtcatc catccgcagg aactgaaaac tgttcctttg atcatttccc ggcaaacgtc
    10441 agtggacagt caactagctg aatggctagg tttcagcata gatcagttat cgatcgtcgg
    10501 aacatataac ttattgtaca atgcttcttt gatggtcgat aacgggactt ttggtgcatt
    10561 agctctagat gggatcatca atacttatgg gacggaaaaa atattcatcc cactcgatcc
    10621 gccattacaa gcttcactga atctcatttg gaaaaaagac cagcctcttt ctcctgctgc
    10681 tcgtaaattt ttagatgtcc taacggagaa atacagttaa gtagagtttt tttccttcgc
    10741 tttcagcttt ttaa
//
LOCUS       DACEXQ010000080        10753 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.080, whole genome shotgun sequence.
ACCESSION   DACEXQ010000080 DACEXQ010000000
VERSION     DACEXQ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10753
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.080"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..252
                     /locus_tag="IXH04_001454"
     CDS             <1..252
                     /locus_tag="IXH04_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAQ1852066.1"
                     /translation="MLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILG
                     GIINAVVQEYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            328..1467
                     /locus_tag="IXH04_001455"
     CDS             328..1467
                     /locus_tag="IXH04_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAQ1852067.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            1460..2260
                     /locus_tag="IXH04_001456"
     CDS             1460..2260
                     /locus_tag="IXH04_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1852068.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            2260..3087
                     /locus_tag="IXH04_001457"
     CDS             2260..3087
                     /locus_tag="IXH04_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1852069.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            3065..3799
                     /locus_tag="IXH04_001458"
     CDS             3065..3799
                     /locus_tag="IXH04_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1852070.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            3899..4765
                     /gene="rfbA"
                     /locus_tag="IXH04_001459"
     CDS             3899..4765
                     /gene="rfbA"
                     /locus_tag="IXH04_001459"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAQ1852071.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            4779..5351
                     /gene="rfbC"
                     /locus_tag="IXH04_001460"
     CDS             4779..5351
                     /gene="rfbC"
                     /locus_tag="IXH04_001460"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAQ1852072.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            5373..6401
                     /gene="rfbB"
                     /locus_tag="IXH04_001461"
     CDS             5373..6401
                     /gene="rfbB"
                     /locus_tag="IXH04_001461"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAQ1852073.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            6499..7350
                     /gene="rfbD"
                     /locus_tag="IXH04_001462"
     CDS             6499..7350
                     /gene="rfbD"
                     /locus_tag="IXH04_001462"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAQ1852074.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            7384..9417
                     /locus_tag="IXH04_001463"
     CDS             7384..9417
                     /locus_tag="IXH04_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852075.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPAKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKIDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKEIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            9461..10741
                     /locus_tag="IXH04_001464"
     CDS             9461..10741
                     /locus_tag="IXH04_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAQ1852076.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIATLLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYGEQPDSAHSSRIQRYH"
BASE COUNT         3295 a         1797 c         2298 g         3363 t
ORIGIN      
        1 atgctgttat cacaagtgtt tagtatctat gctaggtatt ttgcaacgag gatcagcggt
       61 tatcagatca taggaagttt tattgtactc atgctttggc ttaattttgc tgctacgatc
      121 atcattttgg gcggaatcat caatgcagtt gttcaagaat acgtgacggg tcatcaaatc
      181 aaaaaacgtc gagatccaag ccgaaaatgg ttcaataaat tgaaaactaa attttctcat
      241 aaaaaaaagt agaaacaaga gttcttattg taaatttata tcagtaattt ataataatta
      301 tgactcttaa ttaaagtagg tgcccatatg cttgtttctt ttagtatggt tataagattt
      361 tttctgacaa ttttattatc actgatatta acacctgtat tcaaaatcat ttcagtacaa
      421 acaggtatgg tcgacaaacc aaatgaaaga agaataaata aagttccaat gccttcagcg
      481 ggcggactgc ctatctttat ttcatttgtt atatcgactt tattcttatt ccgtaaaatt
      541 atccctcaat cgtatattgt accgatcttg attgcttctt tagtaatcat agtcacagga
      601 gtgctcgatg ataaatatga gctgacacct aaacaaaaaa gtatagggat cttgattgct
      661 tctcttatta tttattttgt agcagatata cgtattgatt ccttcacgct tccttttatt
      721 ggatatatcc aactaggctg gcttagtttt cccgtaacga ttttttggat cttcggtatc
      781 acgaatgcgg tcaatctgat tgacggtttg gatgggctgg cggcagggat ttcattgatc
      841 ggtttaatta cgataggaat cattggatat tttttcctcc atgcttctac agtgtatatt
      901 ccagttgtta ttttctgtct tgtagcaagt attatcggtt tttttcctta caatttttat
      961 ccggcaaaga tttatttagg cgatacagga gctctattcc tagggtttat gatggccgtc
     1021 ctttcactac aagggctgaa gaacgtgact tttgtttcat cgatttcttt gctgatcgtc
     1081 atgggagtgc cggtaacaga cacgtttttt gctatcatta ggagaaaagc aaaccgtgtt
     1141 tctttttcta cagcagataa aaaacatctt catcaccgac taatcagttt aggctttact
     1201 cataaaggtg ctgtcctgac gatttatgcg atggctttga tgttttcttt cacagctatg
     1261 gtcatgaatt atactggaag aatcggaacg atcatattga ttattgccat gttgtttgct
     1321 gcaattttat tatttgaatt gattggtctg atcaatgaga aacatcagtt tattttaaaa
     1381 acattgcgat ttttagggaa taaagagtac cgtacgcaag taaaacagaa atatttgaag
     1441 aaatggaacc gtcatataga tgaataacaa attaaaccag ccgatcctta ttagtattgt
     1501 gacggcaaac agtgataaaa tttttcggac actagataat tttatttcaa ctgtaaaaga
     1561 tgaaacagca gtaaaaatcc gaatttttga caacaattca cttcccgagt acaaaaaaag
     1621 actaacagag tataggaaat atccctacat cgatatctat tttcatgatg aaaattccgg
     1681 atttggttat ggacataatc acaatctgat agctagcgat tctcgctatg gcattatttg
     1741 taacccagat attttagtgg atgaagagac agttacagca ttggtcttgt tattgaaaga
     1801 acatccggaa tgtgcgatgt tagcacctaa gattcttaat gaagatggaa caactcagca
     1861 cctcatccgt gagcagctgg ctgtttttga ttacgttttg cgctttgtgc ctttcaaatg
     1921 ggtcaaaaaa aagtttgaca aacgactggc tgtgtttgaa tgccgaggat tgcctactga
     1981 tgctaatagt tatgtacgga tgatctcagg tagttttatg gtagctgata ttgataaatt
     2041 taaagagata aaggggtttg atgagcgttt cttcatgtac tttgaagaca acgacttgtg
     2101 tttgagtttt gaaaaagcag gttacaagtt attgtacaca cccttttatg cagttatcca
     2161 tttttacggg aagggtgcgc atcgaaatgc aaaactgttt cgcgtgtttt tgacttctat
     2221 gatgaaattt ttcaataagt gggggtggcg ttttttctga tgaaaccaga aatattagta
     2281 gtcgttgtgc tttatcagca actattcagt caatctccag cttatggacc attaagcaaa
     2341 gcacttagcg aaaagcaaat tcagcttatt atttacgata atagtcctca aaggcagcaa
     2401 aatgagctgt ttgaaaaaga aaatatcgtt tatcatcatg atccgcaaaa tcctggttta
     2461 gcggtggctt ataattttgc gttgtctcaa gcagatgaag gaacacgttg tctcgtcact
     2521 ctggatcagg atacgaggct agtcgacgat tattttgaac tattgcgtaa agtaaccttt
     2581 acagatgaat gtgttgctgc agttccgatg attttttctg ggagccgaca agtttctccc
     2641 ctatattcag atcgttatat caatcgtaca gctcaagcag tagaagttga aggcccaaca
     2701 gctgaacgaa tcatggcaat caattcaggt gttgcctggt ctgtcaaatt tctaaaaaag
     2761 attggcgggt ttaatcctgc tttttcttta gattttttgg atcattggct gttttggaaa
     2821 gtggatcagt tggaaaaaac tgtggagatc cttcctgtac gaatggaaca tgatctttcg
     2881 gttttagact atgaaaaagt ttcaagtaat cgataccgtt ctattttaca agcagaaaat
     2941 cgattttatc atgaatatga tcaaaggcat ttatccagtc accgacgaca attattgtta
     3001 agaacagcta aacagttctt gaccgtcaaa aaccggcata tctggcggat gactttgcgc
     3061 tcatatgtta agaattggaa ggtgtgaatt cgtgcgttcg gtttgtatag ctacctttaa
     3121 tggaggcaga tatttaagag agcagttaga tagtatttta tctcagcttt cagcagaaga
     3181 tgaagtagtc atttctgatg atgggtcagt agatgataca tgggatatat taactgaata
     3241 cgcagaaaaa gataagcgaa tccagctttt ttcggggcct cgtcaaggat tgatcgctaa
     3301 ttttggttat gcgatccagc agacgaaagg agatctaatc tttttggcag accaagatga
     3361 tgtttggttg gagacaaaag tgaaaaagat cactgactat tttgagcaga atccggatca
     3421 gctagtagta gtttctgatt taatcattgt ggacagcgag ctgcaagaga tccatgattc
     3481 gtatttttcg tttcgaaatt ccaaagaagg atggcttaat aatgtgataa gaagtcacta
     3541 tattggtgct ggtatggctt tccggtcttc tttaaaaccc gatattttgc cgatcccgtc
     3601 agaagtgccg atgcatgata tgtggattgg tttgctggga ggaaagaaga ctgggttcct
     3661 aagagaaccg ttaactctgt accgaagaca tgatttaaac gcgagtgaga ttgaaacaca
     3721 ttctaaatta cggcaaaaaa tttcttggcg tgttcatctg attacggcgt tgatcaaacg
     3781 aaaatggttt aaacgttgat aaaagatgtt taaatcctta atatttccat gacaaatata
     3841 acaattgatg agataataac aaagtgaaat ttgaaatcaa aaaggagagc agatatccat
     3901 gaagggaatc attcttgctg ggggcagcgg aacacgtctg taccctttaa caaaggcaac
     3961 atcgaaacaa ttgatgccga tttatgacaa accaatgatt tattatccaa tgtctactct
     4021 gatgttggcg ggaatcaaag aaattctgat tatttcaacg ccacaagata caccacgttt
     4081 caaggaatta tttggaaacg gaaatgattt aggactgcag atcgaatatg cagtacaaga
     4141 aagtccagat ggattggcac aagcgtttat cattggagaa gaatttattg gagatgatag
     4201 tgtctgcttg gtattagggg acaatattta ctatggtggc ggtctgtcta aaatgttgca
     4261 acgagcagct tcaaaagata caggcgcgac agtatttggc tatcatgtca atgatccaga
     4321 acgctttggt gtagtagaat ttgatgagaa tatgcaagct ttgtcgattg aagaaaagcc
     4381 tgctcagcca aaatcaaatt acgctgtaac tggcctttat ttttatgaca atgaggtaat
     4441 ttctatcgct aaaggtatca aaccttcaga acgtggagaa ctagagatta cggatgtcaa
     4501 taaagcatat ttggataaag gaaaactatc cgtagaactt atgggacgcg gatttgcttg
     4561 gcttgataca ggaacacatg aatcattgct tgaagcatct acctttatcg aaacaattga
     4621 aaaacgtcag aacttaaaag tggcttgttt ggaagaaatt gcttaccgta tgggctacat
     4681 cgataaggaa caattgctgg cccttgcaca gccattgaaa aaaaatcaat acggtcaata
     4741 tttattgaat ttggccgctg aataaggagt ccttacagat gaaagtaaca gatacaaaat
     4801 taacagatgt caaaatcgta gaaatggacg tctttggcga tcatcgcggt ttcttcacag
     4861 aaagttattc aaaagcaaaa tttgcagaac atggattgaa atatgacttt gttcaagaca
     4921 accattcact atcagctgaa gctggtgtcc ttcgggggct tcatttccaa aaaggagaag
     4981 ctgcacagac aaaattgatt cgggtagtca ctggagcagt tttagatgta gtagtggata
     5041 tccgcaaagg tagcccgact tatggtcaat gggacggatt tatcttatct gaacataatc
     5101 atcgacagtt gcttgtacct aaaggatatg cccatggatt cgttactttg acaccaaatg
     5161 tgaacttcct ttataagtgt gataattact ataatgcaga agcagatggg ggcatcgcat
     5221 ttgatgatcc agatttagcg attgattggc caattgatcc atcaagagcg attatgtcgg
     5281 aaaaagatca ccgtcatccg acattaaaag aatttgaagc agaaaatcca tttgtttacg
     5341 gagaaatcta agaattaagg agaaattttc ttatgaaaaa catcattgtt accggaggtg
     5401 ccggattcat cggctctaat ttcgttcatt acgtggtaaa caatcatcca gaagtgcatg
     5461 tcactgtttt ggataaactg acttatgcag gaaacaaaga gaacttggct ggacttccta
     5521 gcgatcgcgt agaattggtc gtaggagatg ttgcagatgc agaattagtg gaccgtctag
     5581 taaaagaagc ggacgcagtt gttcattacg cggcagaatc ccataacgat aattcattaa
     5641 aagatccttt cccatttgta caaacgaata tcattggtac gtatacactt ttagaagctt
     5701 gccgcaagta tgatgtacgc tatcatcacg tatctactga cgaggtttac ggcgatcttc
     5761 cattacgtga agacctgcca ggacatgggg aaggcgaagg agaaaaattc actgcggaaa
     5821 caccatataa tccttcaagt ccatattctt ctacaaaagc tggttcagat ctgttagtaa
     5881 aagcatgggt gcgttctttt ggcttacgtg caaccatttc aaactgttca aacaactatg
     5941 gtccttatca gcatattgaa aaatttattc ctcgccaagt aacgaatatc ttaagcggta
     6001 ttcgtccaaa attatatggc gaaggaaaga atgttcgtga ctggatccat acgaatgatc
     6061 attcttcagc agtgtggctg attttgacga aaggcagaat tggcgaaacc tatctgatcg
     6121 gtgcagacgg tgaagaagat aataagaccg tgatggaatt gatcttagaa atgatgggtc
     6181 aaccaaaaga tgcgtacgac catgtaaacg accgtgcagg acacgacttg cgttatgcaa
     6241 tcgattcgac aaaattacgg gaagagttag gatggaagcc tgagtttacg aacttccgtg
     6301 atgggttggc tgacacgatc aaatggtacg aagaacatga agactggtgg aaaaaagaaa
     6361 aagaggccgt tgaagcagca tatgccaaaa atggacaata atacagagaa aatgtcactg
     6421 tattaatgga aaaacacatg tgaggcaggc gctcgcctgt ctctttttta aaataaaaat
     6481 caaggagcgg ataatcatat gtttttatta actggaggaa atgggcaact aggtacagaa
     6541 ttgcgccatt tattagatga aaaaaatctg caatatgttt caacagatgc aaaagacttg
     6601 gatatcacag atgccgagaa aacaatggct tacatcacag aattaaaacc tgatgttatt
     6661 ttccattgtg cagcttacac agcagtagat aaagcagaag aagaagcaaa agaattagat
     6721 gagaagatca atgttgatgg tacaagaaat gtagcacttg cagcaaaagc tgtgaatgct
     6781 caattcgtct atatcagtac agactatgtt ttcgatggaa aaaacaaaaa tgtagaatac
     6841 gaagtagacg accaaacgaa tccactaaat gaatatggtc gtacgaaatt attaggcgaa
     6901 aaagcagtag aagagatttt agaagactat tatatcatac gaacttcttg ggttttcggg
     6961 atctatggac acaatttcgt cttcacgatg caacgcctag cggaaacccg cgacaaactg
     7021 actgtagtaa atgaccagtt tgggcgacca acttggacac gtactttggc agaatttatg
     7081 gtatatgtca tcgaagaaaa agcaccattt ggtatttatc atttgtcgaa cgaaaatagc
     7141 tgttcttggt atgaattcgc aaaagaaata ttgaaagata cagatgtaga agttgcacct
     7201 gttacgtcag aagaatttcc gcaaaaagca acacgtccgc agtattcagt catgagcctg
     7261 aaaaagacag aagagttagg atttgtcata ccaacttggc aagaagcact tgcgcaaatg
     7321 ctggaagctg ccaaaaaaat gaataagtaa tttcctttta tcaaatagaa tttggagcat
     7381 gatatggaaa aacaaacaag aagtgcacga cgaaaagaaa agccaaaagc gaaccaggca
     7441 gattcgaatt tggtgcttga cctgtttcta aaactccgct atcttattgg cgttttagtc
     7501 atcgtggtca tcgttacttt taatttaaac ggaagttcac ttggtgtttg ggataaatat
     7561 gtgtcgcaaa gagacgatgg gaaaaaaact gatgtgattt ttggacagaa tcgggaggtg
     7621 cggtcagacg aatggctggt gcagacacct ttttacctct cacaagctga agaggacttt
     7681 ccgttggtga acgaggatta ttcattaaac ggacaaaaca tgatcattgc gtataattct
     7741 cctgcaaagg atattacggt tatcggaaaa ccattcaact ggggattctt tttcctcgga
     7801 agagatcgag gactttcctt ttattgggcg atgaaattag tcgtcatggt cttgcttgga
     7861 ttcgaagtac tgatgatatt aacgaagaga aaaaaagctc tatcgctaat cggtgcattt
     7921 gcgctgacat tttctccagc agtccagtgg tggttcatgc aacatgtggg cgatttgatt
     7981 ttctttactt tgggactgat gactgctttt tatcattatt tctacaatca tgataaaaaa
     8041 tggctacgaa cgttgatgat gcttttggtg gttattttcg gtattgggtt tattttagtg
     8101 atttacccag cccatcaagt gatgctggcg tacttgctga ttttttattt tgttggcctg
     8161 cttatttact atagcaagaa agtttcatgg gatctgttgg atgtagtgtt gattggaggg
     8221 gcagttttat ttattggcgg agtcatggtt catttctggc taacgtcaaa agacgcattg
     8281 ttagcttctt tgaatacctt atatccagga aaacgggtaa gtactggagg aaagtggacg
     8341 attggcgaat ttttttcttt ccttacaaat tggaaaattc cttttgaaga tatcaactat
     8401 tcgaataact ctgaagtggc cctgttctat cacttttttc caactgtatt cctagcaagt
     8461 ccatttgtgt tatttggcaa aaaagatagc gaacaaaagt tactgggacg tattttgatg
     8521 attttttgtc tgttttctat cttttggatt accgttgggt tgccaaaatg gctagcagaa
     8581 atcactctgc tgtcttatgt tcctccatta agagccatcc ttacttttag ttttgcggct
     8641 tgtttattat cgatctggtt tatcgcttat atttggagag aagatgttat tcctatttgg
     8701 tataaattca ttttgttggc agtaaatgca tggtcttatt tctatgcgat tacgcattcg
     8761 aaaatagata attatttttc ggattttgaa accatcgcag ttgctgtgtt gggaacagtg
     8821 attttagcat ttgcattatt tggctggaga cgcttgtttt acctcgcttt tgccgcctta
     8881 gttatcgtat ctggcttttt cgtcaatcca gtcgtagaag gtacaggagc gatttttgaa
     8941 aaaacactgg caaaagaaat ccaaaaaatc gatcaacaag atcctggtaa agtttggttg
     9001 tctgaagatg aattatataa cttcacccca acattgggag tgaaatcctt caatacagta
     9061 cgcttttatc cagatatagc tgcatggaag gaaatcgatc cgaaagaaga atacgagaag
     9121 tattataatc ggtatgctca tacaagagct tttatcgcta aggaagatac aaagttcatt
     9181 ttagatcgtc cagatatgtt ttcggtcacg ttgaattttg aagacgcaga aaaattaggg
     9241 atcgactatg tgatcagtaa acgaccatta actgattaca accaattata tgaagcgcag
     9301 ttcacgcagc tatatggacc ggataaagac ggttatatga tttttaaagt agaatatccg
     9361 gattatccag atgtaccaca aacacaaact caggaaaatc agctagtcgt acagtaatag
     9421 gctagctgag aataatcctg aaatattagt agaaaggaga gtggagataa ttcaagcaat
     9481 cgtacaaaaa tttgtcaaca aaatgagtgt cggtttcttg cttttgttgc tgacgctagt
     9541 cttttttgtt tggacagcag ggttaggttt tccagcaact ttgtttatat ataacaatag
     9601 ttcattactt atcgctttta gtgtggctat tttgctcatt ttaaatgttt ataaagcaaa
     9661 aggcgtcgat tttctttatc taggtgttgc gcttgccctt ttcattttct tttcttactt
     9721 tcgggaatta agaaatgagt ctttatttgg agatttactg tttcctttga ttattttatt
     9781 ttgtttagta attaaatgga tcgagtggac aaaatgggat cgcggtattt atctgattgt
     9841 ctttacggtc ttcctcggaa tcacgttata tcgtgtattt acagagatcc aagtaccaga
     9901 aggacaaagc atctgggctc caggcaattc gctttctgat atttggatca atacgaatac
     9961 aatcggaagt tctttgatga ctcttgcttt attgatcagt ggatttgcca gttcttttga
    10021 acgatggtat gtacgaatca tcggtgcagt agcagttgtt gcagcttttc tcgggatttg
    10081 ggtctgccaa tctagaggtg cattagtcgc gttgattgtt tttgcgatat tggatctttt
    10141 acctaaatcg ttgatgcgtg tcattcgtgc gccatttatt gcttacacgg ttacgattct
    10201 tttagctttg ccgatttctt atctggcggc tgtctctgaa aaagtcaatt tgtttaccgg
    10261 tcgagaagat atttggcata aattctatca gacgctagga gaaaaatctg aacaagtttt
    10321 actaggaatg aaaacattta tcttccaacg aggaaaccaa tttttaggaa accacaatag
    10381 ttataattct attttgaata tctatggatt gataggattt gggattgcta ctttgctgct
    10441 tatccttttt attggtcgct taacgttgaa agcagattta tccaacgggc agatgacttt
    10501 tatctgggcc ttttttgcgg tgatgatgca gtcatttatg gaggatacat tgacttcata
    10561 cccatgggtt ccgattattt atatcctgtt ggctatggcc gttcatcgat atgatgaacc
    10621 agaagttgcg gaggaattct atgttatgga agaggcatat gacgatatga cttatgatga
    10681 tacatcttat ggggaacaac cagatagcgc tcattcttcc aggatccaac gctatcattg
    10741 ataggcacaa aat
//
LOCUS       DACEXQ010000081        10640 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.081, whole genome shotgun sequence.
ACCESSION   DACEXQ010000081 DACEXQ010000000
VERSION     DACEXQ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.081"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(94..969)
                     /locus_tag="IXH04_001465"
     CDS             complement(94..969)
                     /locus_tag="IXH04_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAQ1852077.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(1051..1842)
                     /gene="codY"
                     /locus_tag="IXH04_001466"
     CDS             complement(1051..1842)
                     /gene="codY"
                     /locus_tag="IXH04_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAQ1852078.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(1860..3260)
                     /gene="hslU"
                     /locus_tag="IXH04_001467"
     CDS             complement(1860..3260)
                     /gene="hslU"
                     /locus_tag="IXH04_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAQ1852079.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(3274..3822)
                     /gene="hslV"
                     /locus_tag="IXH04_001468"
     CDS             complement(3274..3822)
                     /gene="hslV"
                     /locus_tag="IXH04_001468"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAQ1852080.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(3859..4764)
                     /gene="xerC"
                     /locus_tag="IXH04_001469"
     CDS             complement(3859..4764)
                     /gene="xerC"
                     /locus_tag="IXH04_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAQ1852081.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(4962..6338)
                     /gene="trmFO"
                     /locus_tag="IXH04_001470"
     CDS             complement(4962..6338)
                     /gene="trmFO"
                     /locus_tag="IXH04_001470"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAQ1852082.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(6307..8385)
                     /gene="topA"
                     /locus_tag="IXH04_001471"
     CDS             complement(6307..8385)
                     /gene="topA"
                     /locus_tag="IXH04_001471"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAQ1852083.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(8507..9364)
                     /gene="dprA"
                     /locus_tag="IXH04_001472"
     CDS             complement(8507..9364)
                     /gene="dprA"
                     /locus_tag="IXH04_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAQ1852084.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(9420..10187)
                     /locus_tag="IXH04_001473"
     CDS             complement(9420..10187)
                     /locus_tag="IXH04_001473"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAQ1852085.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(10180..>10640)
                     /gene="rbgA"
                     /locus_tag="IXH04_001474"
                     /gene_synonym="ylqF"
     CDS             complement(10180..>10640)
                     /gene="rbgA"
                     /locus_tag="IXH04_001474"
                     /gene_synonym="ylqF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="essential GTPase; functions in ribosome assembly;
                     binds a unique part of the 23S rRNA; interacts with
                     ribosomal protein L25(Ctc); Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAQ1852086.1"
                     /translation="TLMNRLVGKKIAQTGNKPGVTKGQQWLRLGNELELLDTPGILWP
                     KFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYSGRISDRYGLSIEQEEKLA
                     PELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDRCEEMEQKSDD"
BASE COUNT         2759 a         2368 c         1860 g         3653 t
ORIGIN      
        1 aaatcgatgt cacactccct tattacttac aggatagctt ttttacacac cttggtaaac
       61 ggtgttccct gctaacttct cggtcacaac ctcttacttc actgtgatgg aatattttgt
      121 cttgaagatt tcatgggcac ctagttgatt gatccctact ttttccatca agtttccact
      181 actgttcacc gtgtcagcga ttccgcacca cggttcgata caaacgaaag gcgcttcttt
      241 tggataagga gaccagatcc ctacataagg catatcttta tagcttaaag ttacgctatg
      301 ggaagatttt tcacttcgaa tggcgtacgc atttagaccc cttgtttcaa aaaccaaggc
      361 atcttgctca aaaagttcac gattcaaagc cagattcgta tttgtctgac ctaacgtctt
      421 ttgatccata tcgatgaatg ggcctttcaa tggtaggcgt aatcttgatt tcaatggaga
      481 aaatgccaga tagaaatctt caaatgacaa tccttcttca agaggtacat tgaaagcagg
      541 atgtccaccg atagaaaaga acatttcttt ttcaccggta tttgccactt gataatgaac
      601 cgtgatgccg tctcctccta attcatagct gatcactaat tcaaaatcaa atggatactt
      661 ttctaatgtc tccggcgtac tccttaagat gaaactagcc atttcttgtc catgttcaat
      721 gacttcaaaa tccatatccc gagcaaatcc gtgttgcccc atttgatatg ttttcccttg
      781 ataaacatac tgatcatctt ttaaacgtcc aacaaaaggg aataaaaccg gtgcatgtct
      841 gccccaatac gctggatcac cttgccagat atactctaaa ccatgttctt ttgatttcag
      901 actgatcaat tccgcaccgt gttcagcgat ttcggcgatc agctggtcat tttgaataat
      961 aacagtcatt gccattcctc caacttcgac ttacttttta aaatgggaag gctggaacaa
     1021 acatttctat ctgccccagt ccccttactt ctaataactg tgttttgcta actcgtcttt
     1081 aaaacgggaa ttcaatactt ttaaataagt tcccttcatg cctaacgaac gagattcaat
     1141 gattccagct gattccaatt ttcgtaaagc attgacgatc acagaacgag tgatgccgat
     1201 actatcggca atattagaag cagtcagacg accttcatct ccatccaatg cttcaaagat
     1261 agcttgtaca gccttcaatt cgctataaga taatgtgttg attgccattt ggacagccgt
     1321 tgcactccgt acatctgctt cgatgcttcg agacttctga taaaggatct gcataccgac
     1381 aaccgtagct ccatattctg ctaagactag atcgtcatct gcaaacgatt gttccatacg
     1441 agataagata atcgtaccca aacgttcgcc agctccaaag atcggaacga cagtcgtcag
     1501 accaaatgga tatttctctc ttgactctac cggaaatgca gtaatatcac tagtaattgg
     1561 gatattagct tcagttttca tcaactgtgc cactgtatca gtataagatt caggaaattg
     1621 tttctcaaca aacattgttt tgattcgttc attgttcaca tcgtgacgtt cattataacc
     1681 tagtaatact ccgtcacttc ctatgatata ggtgttgctg tcaagaatac cacctagtgt
     1741 gacagccatt ttatcgtaag gcaaatctgc ttgcatgtca aaagtattct tttgctgcaa
     1801 aagtgtgttt atcttgcggg ttttcgttag caagtctgtc attgtcgtcc tccgattttt
     1861 tataaaatat agcgacttaa atcttcattt gccgcaattg agccaagttt gtcatccaca
     1921 tatgcttctg tgatcgttat ttctcccatt tgcatatctg gtgcttcaaa taatagatct
     1981 tctagtagtt tttccaaaat ggtatgcagt ctgcgtgcgc cgatattatc tgtgtcacgg
     2041 ttgacatcga aagcgatttg agcgatccgt tcaattgctt cttttgtaaa cgtaactgac
     2101 acattttctg taccgatcaa cgcgacgtat tgttttacta aagcattatt tggttcggtc
     2161 agaatcttca caaaatcttc agctgtcaaa tcatctaatt ctacccgtat cgggaagcgt
     2221 ccttgaagtt ctggaattag atcacttggt ttagacaaat ggaaagctcc tgaagcaatg
     2281 aataggacgt gatctgtttg gatgatcccg tattttgtgt ttacttgtga accttcgaca
     2341 attggcaata tgtctctttg gacgccttct ctggatactt ctccagattg ctgtgattta
     2401 gaagtgatct tatcgatttc atcgataaaa ataattccac tttcttccgc taatttgatt
     2461 gcctcactat ggatatcagc atccttgact aattttgctg attcttcctt gatcaataac
     2521 tctaaagctt ctttgactgt taccgtccgt tccacttttt tcttcggtgt caaagcacct
     2581 agagtttcat ttaggtcgat ccccatttgc tccaatccat tgttcatcat tggtgcaggt
     2641 ttcttttggt cttcaacttc aatggttact tcacgactat ccagtaaacc cttttgtagt
     2701 tgttccaaga ctgtacggcg attgattcga atatcttctg tcacttcttc ttgcggttca
     2761 gcggtatttt gctgactagc attaaaaaga ttcatcattt gttcaaattg attagtggat
     2821 tgtttctgtt cttttttgat accaggtacc aagattttta ccaaacgttt ttctgcattt
     2881 tttttagcac ttgtgtatac acggctgtac tgctcttttt ctacgatttg gatcgcattt
     2941 tctactaaat cccgaaccat tgattctaca tctcttccga cataaccgac ttcagtaaat
     3001 tttgttgctt cgactttgac aaatggtgca ttgacgattt ttgccaaacg tcgtgcgatt
     3061 tctgttttac cgacccctgt aggtccgatc atcagcatat tttttggtgt aatatcttgt
     3121 tgcatttttt catctaactg caaacggcga tagcggttac gcaaagcaac ggcaacagat
     3181 ttttttgctg cgtgctgtcc tacgatatat tcatctaatt cacttacgat ttgtcttggt
     3241 gtttgtttta attcatacat atctactcac ctcttacagt tcttctacga taatattatg
     3301 attagtaaag atacagatat ctgcggcaat atttaacgca ttttctgcaa tttctttcgc
     3361 actcatgtcg ttttgagcat agtttttcat tgctcgggct gcagccaacg caaaattccc
     3421 tcctgatccg atagctaaga taccatcatc tggtgtgata acttctcctg tacctgaaac
     3481 aagaagcatt tctttatcat tcattacgat cagcatcgct tcaagttttt gcatagattg
     3541 ctgtgtccgc cattcttgag ctaattcgac agctgcgcgt tgtagattcc cgttatattc
     3601 gtttagtttg ccttcaaatt tttcttctaa agtaaacgca tctgcaacac ttccggcgaa
     3661 acctacaaca acttctccat tgtagatacg tcgtactttc tttgcagtgc ctttcatgac
     3721 aacagattca cccattgtta cttggccatc tccagccata gcaaatttcc cgtctttttc
     3781 gacggcacag atagtggttg aatgaaatgg tgattcgacc atgtttgttt cctcctaaat
     3841 ttgttctttt ccttattttt acgctcttgg atggaaagaa cgatagtttt tttgtaaact
     3901 ttcttttgtt acatgggcat agatctgggt agtcgataaa ttggcatgtc ctagcaattc
     3961 ttggactgtc cgcaaatctg ccccattatt taataaatga gtagcaaatg tatggcgcag
     4021 catgtgagga tggatatcac tatccaaact gctttttttg atcatttgat tcaagacata
     4081 ttcaattcca gtaggcgtga ttggttttcc atgatgattc acaaaaacga tatcatgctc
     4141 ttcgtgatat tttgtcatca atacctggcg gccttccctg aaatattctt gcagagcatc
     4201 ttgagcaaaa gaacctagag gagcatatct ctctttgttc cctttaccat ggatcaacat
     4261 gacctctgaa tcaaaatcaa tgtctgatac cgacaaactg ctacactcac ttaaacggat
     4321 gcctgacccg taaaggactt ccagtaaagc gcggttcctc aaatccaaag gactatctcc
     4381 tgctacgctg tcaaaaagtg cttgcatttc attttcataa aaaaaacgtg gcagtctcaa
     4441 atttttcttt ttgagatgaa cgtaagaaaa tggattttct tttatcactt cttctttgag
     4501 aagatactga taaaacgaac gtaggctggc tatcttgcga ctgatagaat ttctgctata
     4561 gctaggacga tgatcagcta atgcactcaa atagacacga accgtaaaat attccgcctt
     4621 cagcagatca gcgtctccag attcctccaa aaaatgtttg aaatggagaa tgtcttcttc
     4681 atatgcttct ttcgtttttt ctgaatatcc tctttcgaca atcaaataac gcaaaaattt
     4741 ttgttcccac gtggcttctg tcattttctc accttccttt tgtcccagag aataatttag
     4801 cacagcgttt ataaaaagac aaataaattg tcagaattta accaatattt ctaagtttgt
     4861 tcacaattgg cgcaattcct cacattttgt ttgagaaatt cttaagaaaa acgcaagaaa
     4921 aagagcgaga ctatccgtct caccctcttc atggagtttc cttactcatt tgggattgca
     4981 gttttttcta attctgtttt tattgcctca ttagctttta aagctcgttg tgcaattgct
     5041 tcatatcttg cttttttgtc tcgaatgcgc tctggcagtt caggaaacag accatagttg
     5101 gcattcatcg gttggaaatg ttttccttcc gcatgagtaa tgtagtaagc catacttcca
     5161 atagctgttt cttgtggaaa ctcgactggt tccatttctt ttgctaagcg tgcggcgttg
     5221 attccagcaa gtagtccgct cgctgcactt tcaacatagc cttctacacc agtcatctga
     5281 cctgcaaaga acagatcttc gcgtttcgtt gattggtaag tcggtttcag caattctggc
     5341 gaattcataa agctgtttcg gtgcatcaca ccatagcgaa cgaattcagc attttctaac
     5401 ccaggaatca tccggaaaac acgtttttgc tcgccccatt tcaagtgagt ctgaaagccc
     5461 acgatgttgt ataaagaagc tgcagcattg tcttgacgca gctggataac agcataagga
     5521 cgtttgccag ttttaggatc ttccaatcca actggtttca taggtccgaa aagcatcgtt
     5581 ttgaatcctc gctgtgccat tacttcaatt ggcatacatc cttcaaaata tttttctttc
     5641 tcaaaagatt taagttctgc cacttcggca ttgattagtg cctcatggaa agctttgaat
     5701 tcctcttctg tcatcggaca gttcaagtaa gctgcttctc ctttgtcata gcgggacttc
     5761 aaatatactt tatccatatc gatcgtagct ttgtctacga ttggtgcagc tgcatcatag
     5821 aaataaaaac catctgaacc attgaatgct ttgatgcttt ctgctaatgg atgagaagtc
     5881 aaaggtcctg ttgctactac ggtgattcct tccggtattt cagtaatttc ttcgtttatc
     5941 actgttacta atggatgctc tttgactttt cgggtaattg tttcagaaaa agaatcgcgg
     6001 tcaacagcta aggcaccacc agctggcact gctgtttcat ctgccgaagt gattactacc
     6061 gaattcaatc ggcgcatttc ttctttcaat aaccctactg cattagccaa actgtttcct
     6121 cttaatgaat tggaacatac tagttcagca aaattacttg tgtgatgcgc ctcagtcgat
     6181 ttgactggac gcatttcata taaacggaca gggactcctg cttgtgcgat ctgccaagca
     6241 gcttcacttc cggcaagccc tgctccaaca acatttacca ttttcaatga aagaacctct
     6301 tttctattat ttttggatgt cttcttcgta atctccattt acacagacta cttgtttacc
     6361 gcctttgact ttcttctcga ccagatatcc gtcacatttt ggacaatttc tgcccactgg
     6421 tttatcccaa gaagtgaatt cacattctgg gaaacgggaa caaccataga atagtctgtt
     6481 tttcttagat tttcgttcaa tgacttgtcc ttcatgacat actgggcaag tcacaccgat
     6541 ttcttttacg attggtttcg tattccgaca atcaggaaaa ttactgcaag cataaaactt
     6601 cccatatttc cctaatttta tcaccatcgg atgtccacag acatcacaat cgaatcctgc
     6661 tggttcatct ttgatttgaa ttttttcgat tttttcttcc gcattcgtca gttctttttc
     6721 aaacggttta tagaaacgat caaccacttt gacccacgct tccgtgcctt cttcaatttt
     6781 atccaaatct ccttccattt ctgctgtgaa atggatatcg acgatttgag ggaagaattc
     6841 acagattaat gaattgacaa tttctcctag ttcagtcggc tcaaatcttt tttgtgtcaa
     6901 ttttacatag tagcgtcttt ggatcgtatc aagtgttggt gcataagtgg aaggtcgccc
     6961 taccccgttt tcttctagtg cacgaatcaa cgtagcctca ctaaaacgtg ctggtggttg
     7021 agtaaaatgt tgttttggtt caatatcaac agattggact acatcgcctt cttcaagttc
     7081 tggaagaata ttttctttct cttctttgcc gtcatctcta ccttccacat acacttgcat
     7141 gaatcctttg aatttgattt ttgaaccgtt cgcaatgaag atgacaccat tttgttctaa
     7201 tgtcactttc attgtatcta aaatagctgg tgtcatctga ctagccacaa aacgtgacca
     7261 gatcaatgta taaagtttca attgatcttt atctaaatat tgtttgagtt catctggtgt
     7321 acgaagcaca ctcgttggac gcacagcttc atgggcatct tgtgcgcctt gagcattttt
     7381 gatctttcgg tgatcatgtc tagaaaattc ttttccatac gtatcttcaa taaatgctgc
     7441 ggcctctgct tttgctgtat cggcaatccg ggtagaatca gtacgcatgt atgtaatcaa
     7501 gccgactgtt ccttgtttac ccaaagtgat tccttcatat aattgctgtg ctaccatcat
     7561 ggtttttctt gttcgaaagt ttaacttacg ggcagcttct tgttgcaggc tacttgtagt
     7621 gaaaggattc gcagggtttc ttttgcgctc tttcttttct acttttttaa cttcgaagtc
     7681 tgttccatca attctagcag tgacttcttt tacactttgt gcatctggta atttcttttt
     7741 cttaccgtcg attccccaga aatttgcttt gaatttcttg cgagcttttt taaaattacc
     7801 gtcgatgctc caatattctt ctggaacaaa ttctcggatc tctttttcac ggtcaataat
     7861 aatttttaaa gcgacagatt ggacacgtcc tgcacttaac cctttcttga ccttacgcca
     7921 gagaatagga ctgatcgaat aaccaacgat tctgtctaag gtacgacgag cttgctgtgc
     7981 atctactaaa tctacatcga tcgttcttgg ttccttaaaa gctgctttga ctgcgtcttt
     8041 tgtgatttca ttgaagacca cccgattttt ttctttcaga tccaacccaa gtaggtacgc
     8101 taaatgccaa gcaatcgctt ctccttctct atccgggtca cttgccaagt aaactttttg
     8161 agcttttttt gctgcggctt tcaggctttt gatgacatcg ccttttccgc gtatagaaat
     8221 ataatggggt tcataattgt tttcaaaatc gatacccatt ttacttttcg gtaaatctct
     8281 tatatggcca acactggcca ttactttata gtttcgtcca agatatttct caattgtctt
     8341 ggcttttgca ggtgattcta caatcactaa atatttatac gccattactg aatggactcc
     8401 tttccattgt cgtgttccta ctattaaaag aggattcata caaatcgtag ctaatcatat
     8461 ccattcagaa aacgtttgtc aagaaaagca aatgaaaagt atggtgttat tctttaaatt
     8521 ctgccaagat atcttcaccc gaaaccgtgc aaactgcacc gtcttggatc aaatgatgac
     8581 atccggtaga atgttctgtt aaaatatttc ctggaacaga aaaaactgtt cttccaagat
     8641 ccaatgcttg ctgtgctgtt atcagagagc cgcttttttt cttggcttca aagacacatg
     8701 ttcctttaga caaagctgcg ataatccgat ttctcattgg aaaacaatgc tttttcgggc
     8761 cggattgagg aggatattca ctcaaaagaa gatgatcatt tgcaatcacc tcttgcaagt
     8821 cacgattttc agcaggatag acttgctcta tccctgttcc aatgactgcg attgtccgtc
     8881 caccatgaga gattgctgct tggtgaccgt atgtatctat ccctttcgcg cagccactga
     8941 cgatcacata tccagcttct atcactggtt ttagtaaata ataaactgtt tttttggcta
     9001 aagcagaagt gtgtcttcca ccaacaacgc ttaacatgtt acaagaaagt aaagatagat
     9061 caccattata aaacagtaca attggcggat aggtcaagtg aagcagctga gggggatatt
     9121 tgggatcaaa gcaagtgata aacgactgtt tcgattggat tttgccccag ttagcgtgaa
     9181 tttcctgcca acttttgcga aacagctccc ggtgtttggt tattttacct atcttaatga
     9241 tgtcttcaaa tccgatttct ttataaggat actgcatcat ccagttagca agtgcccatt
     9301 ttccttgact actgattcct tttgtataag caagtttcaa taaattttct tctatttgat
     9361 acatcataaa accgcctttc gtctctcttt ctaataagat acgtaaaagc ggctgttttt
     9421 tattgtaaat atttttttac aggtgcaaaa cttttacgat ggattggcgt aattccttgt
     9481 cgttcaagtc cttctaagtg ttcctttgtt ccataacctg cattatgcga gaaaccatat
     9541 ccaggataaa gcacgtcata ttctttcatc aattggtctc gatatacttt agcaatgata
     9601 cttgcagcag caatcgacac agatcgtgca tcccctttga ttattttttc ctgctgaata
     9661 ggcaaatcca aggtcatcgc atcgatcaac aaatagcttg gtacaatgga caatttttct
     9721 actgcttgga tcatcgctaa tttacttgct tcataaatat tcacatcatc gattgtctgc
     9781 tgatcaacga tcccgatccc gattgccagg gctttttctt gaacttgttg ataaagaagt
     9841 tctcgctttt tctctgataa ttgtttggag tcattcagcc ctaatatgat ctcatttttc
     9901 ggcaagataa ctgcagctgc cacgacaggt ccagctaatg gtcctcttcc tacttcatca
     9961 atgccggcaa tccattggtg accttgtgcg tacccttgct tttcaaagca aaacatttct
    10021 tcatattttt ccttaattaa tgcttccttt ttcaatcgtc gttcaaaaga agccagtgct
    10081 tgctgcacgc ctttgcgtgg atcatttctt aatgattcta gtgtaggatc atctattctc
    10141 tgtatgcttt tcaatcgttc tttgatactt tggatggatt cagtcatcac ttttttgctc
    10201 catttcttcg caacgatcca gtgtgtatcg tccaagtttt ccttgacgaa tatcatgcac
    10261 gactaattcg ctgccacgat catagtcttc tttataccca cgttttgctg taatatccat
    10321 cagtaattcc ggtgccaatt tttcttcttg ctcgattgat aaaccataac gatcgctgat
    10381 tcttcctgaa taatatcggg caaaaaagtc taatccgtaa atcgctaaat catctaaatg
    10441 gacaagctgg tctttgatcg cgcctgtcag cgctagtttt ttgccgattt cttcatcttc
    10501 aaatttgggc cataaaatcc ctggtgtgtc taacaattcc aactcattac ctaaacgaag
    10561 ccactgctga ccttttgtta cgccaggctt atttcctgtc tgtgcaattt tcttgcctac
    10621 tagtcgattc atcaacgtag
//
LOCUS       DACEXQ010000082        10208 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.082, whole genome shotgun sequence.
ACCESSION   DACEXQ010000082 DACEXQ010000000
VERSION     DACEXQ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10208
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.082"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..172)
                     /locus_tag="IXH04_001475"
     CDS             complement(<1..172)
                     /locus_tag="IXH04_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HAQ1852087.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACK"
     gene            complement(302..1813)
                     /locus_tag="IXH04_001476"
     CDS             complement(302..1813)
                     /locus_tag="IXH04_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ1852088.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(2135..3487)
                     /locus_tag="IXH04_001477"
     CDS             complement(2135..3487)
                     /locus_tag="IXH04_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAQ1852089.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLAATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(3640..4716)
                     /locus_tag="IXH04_001478"
     CDS             complement(3640..4716)
                     /locus_tag="IXH04_001478"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAQ1852090.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            4912..6657
                     /locus_tag="IXH04_001479"
     CDS             4912..6657
                     /locus_tag="IXH04_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAQ1852091.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(6937..7374)
                     /locus_tag="IXH04_001480"
     CDS             complement(6937..7374)
                     /locus_tag="IXH04_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAQ1852092.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(7358..8731)
                     /locus_tag="IXH04_001481"
     CDS             complement(7358..8731)
                     /locus_tag="IXH04_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852093.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(9053..9541)
                     /locus_tag="IXH04_001482"
     CDS             complement(9053..9541)
                     /locus_tag="IXH04_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1852094.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(9563..10165)
                     /locus_tag="IXH04_001483"
     CDS             complement(9563..10165)
                     /locus_tag="IXH04_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ1852095.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         2990 a         2176 c         1839 g         3203 t
ORIGIN      
        1 ctttgcaagc gtaacgcccg cctaaaaatt ctacttggcg atgaaaaggc aaggactgga
       61 atagtttcat ttcatcagag gtcaagattc gtgcaatgaa ttttggtttt tcttcgatca
      121 gccgagtgat cctaggaagt tcgactgcat cgatcccaat tccttttatc atatgctttt
      181 ctcttttcaa taaatttaga acactctcat tctaccaaat tataaccatt ttgccacaaa
      241 taagtccaca aataacaaaa atgaaagttt ctcttaaaaa agagaaactt tcattttatg
      301 tttaatcgtt atttgaacgg attacaaatc cgcgtttttt atcattgtga cgtttaccgc
      361 catctttttg gctgttatta tttttgctat aaccgccttt tgatttgttt ccacgataat
      421 taccgccgtc acgacgatta cggttgtttc cgccaccacg tttgccattt ttattgtatc
      481 cttttttctg cattggcaat gggcgttctg gtgtgatctt cactggcact gcatcagctg
      541 gatcttttgc tgtcgttttt agcaaaagtg caactaaatc ttgtgcagaa tattcttcta
      601 ataatttatc tgctgtttgc aggtatttat ctagaccatt ttctgcaagt tttgtttcga
      661 tttgttcaac tgcagcacct aattgacctt tgaatgcttc tttttcggtt ggtggacgta
      721 aagtcgtcat ccgttttttc gtcaagtttt caatcacatg caagtagtcc atttcatttg
      781 gtgtaacgaa agtcacagac ataccgcctt ttccggcacg gcctgtacgt ccgatacggt
      841 gaacgtagct ttctggatct tgagggatat catagttgta cacatgagtg acacctgaaa
      901 tatctaatcc acgtgcagca acgtctgtcg ctacaaggat atctaaatga ccgctcttaa
      961 atgaacgcaa tacgctcatt cgtttttgtt gagacaaatc gccatggatt ccttctgcac
     1021 ggtaaccgcg tgcttctaat ccgcgtgcca attcgtctac acggcgtttt gtacgtccga
     1081 agacgattgt taattcaggt gtttgaacat caaacaagcg agtcatgata tcgaattttt
     1141 cgtattcttt tgcgcggaca tagtattgat cgatcaagtc agctgtcatt tctttcgctt
     1201 tgatcttcac atgatgtgga tttttcatga atttaacgcc aatattcttg atagctggcg
     1261 gcatcgttgc tgagaaaagt aatgtttgtc tttgatcagg aacttgtgaa atgatttttt
     1321 cgatatcttc caagaagccc atattcaaca tttcatctgc ttcatccaaa actaatgttt
     1381 gaacggttcc aagtttcaat gtgtggcggt taatgtggtc aagcatacgt cctggtgtac
     1441 ccacaacgat atgtggacga tctttcaagc cacggatctg acggccaata tctgctccgc
     1501 cgtaaacggc ttgtacacgt acttttttgt cgcgccctaa acgatataat tcttcttgtg
     1561 tttggatagc taattcacgt gttggtgcga taactaaacc ttgtaattcg tgacgatctg
     1621 gatcgatttt ttctaacatt ggtaatccaa atgcagctgt tttccctgta cctgtctgtg
     1681 cttgtccaat aacgtctttt ccagctaatg ctagtggaat cgtttctgct tggataggcg
     1741 ttgcttcttc aaaaccagcg cgttcgactg atttcaacaa ttctggtgat aattctagtt
     1801 ctttaaattt caaatgaatc ctcctatatg gttgttaact gaggatgtag cttttttgct
     1861 accccgtcga tttgttcggt caaagtgtat tgtttctttt tgacagtggc cacgcacgtt
     1921 tttatccatt cttagcccct gtggcaccac cgaaaaatca ctttgcgaag tttaggcatt
     1981 agaaaagcca aacgggtcgc gttcagcttt atcattatta tgacatctgg cttcaacaag
     2041 ttgtttctat ggcaaaacca gtccaatttc tcttcatctt tcggcaatag gttattctaa
     2101 catagttttt cgttttctgc aagtttacag atttttattt catttgctga agttgttcta
     2161 ccacttcaaa taagcccatg ctgttgctgc cttttagcat aatactatct gttgctgcaa
     2221 gattttgttt gatagcatca atcaatttat tcttttcttt ttcaaaatag aacacaggta
     2281 gttgggggta tttttggttc aattcttctt ttagtgcatg catttgttcc ccataaagga
     2341 aaatcatggc gaatttctca ggatcgatat gttcactcat ttgcgcatgc atatttaaag
     2401 actctttccc taattccagc atatctgcta acacagcgat tctgcgtcct tttgttggca
     2461 gatttttaaa agtatctaat accagtccca tggctgttgg atttgcattg tagacatcgc
     2521 tcaaaatagc tgcaccattt cctgccgtta gccattcggt tctgttttga gtgatagaaa
     2581 catgggcgag cccttggcgt atctcaggta tggacagtcc aaagaagcgc ccgatcgtat
     2641 acgcaatcaa agcattcgtt acattataac ttccaggcag cgggatcgta aatgtttctc
     2701 cttctactac aaaatcggtt tgtgcttttc cttttgctgt cacttccgca tgaacatcgc
     2761 catttccaat accgaatgtt gtgactgttt gtactaatgt tgccgttaaa ggtgtcagca
     2821 acggttcatc tgcgggcaca acgagtaatc cgtctgatac aagtccgtca gtgatttcca
     2881 ttttggcttt agcgattcct tctcgcgacc caagattttc tatgtgtgct tcaccgatca
     2941 ttgtgattgc tgcagctttt ggttgagcca taagtgaaag ttccgtgatt tcattggcgt
     3001 gatccatgcc catttccaaa acgatctttt ctgtatcttg gggcatgtgc aaaatcgtgt
     3061 aaggaagacc aatattattg ttgtaatttc cttgcgtctt atatgttttg tatttttcag
     3121 acaagacaga cgcggttaga tctttcgtgg tggttttacc attgcttcct gtaattgcaa
     3181 cgacttctgc gcctactttt tccaagtaat aggttgccaa ttgctgcatg gctttcaaag
     3241 gatctgctac aggaataact ggaaaatttt ttggagcttg ttcaacaggc aagctccaaa
     3301 atgaagcaga tgctccgttt gtgatcgctt gctggataaa ttcatgtcca tctcgcgtac
     3361 cagcaagagg aacgaataaa cttccttcgg ttatcttccg gctgtcaaat tcgacattgg
     3421 taatctttgt cagtgcagag cattcgctgc ccacggcttg tgcaatttct tgtattgtta
     3481 gattcattga gatctctcct aaaaaaggtt gtgaaaaaag cgtttggcct aaacggcggg
     3541 ttcgcatgac tctcacttcc aagtaaattt ctctattcgt atttagaagc tagaatgaac
     3601 gcggttacgc aatcactcca gcctcttctt tatttctctt tattcatttt tttcaaaaaa
     3661 agattgacgc tgatggtatc gattcattcc taactggatc agttcttcaa tcaaatcacc
     3721 gtatttcaag cccatatttt cccataagag tgggtacata ctgaactccg taaatcctgg
     3781 catagagttt aattcattca ggaataattc atttttattt gtcaaaaaga aatcgcaccg
     3841 gctcaatccg cttccaccta acatcgtgta agctaacttc gcgtactctt gcgctttttg
     3901 ataaacttct tctggcactt cggctggaat ctgcatttcg attttattat tgatatattt
     3961 tgcttcataa tcatagaatg ctacgtcttt tacgacttca ccaggcaaag tcgtccgaac
     4021 gtcttcattt cctaatacag caacttcgat ttcgcgcgct tcaattcctt gttcaacgat
     4081 tgctcgagaa tcatactgat aggctgttgc taaagcattt tgcagctctt ctcggttttc
     4141 tgcttttgta atgccgacac tagaacccat attcgccggt ttgacaaaca tcggataaag
     4201 caaagaacct tcacattgat caaatacttt tttaggattt tctttccatt gattcttaag
     4261 tactggtaca taaggaactt gcggcacacc agcagcttgt aaaatatact tggtcatgat
     4321 tttatccatg gcacatgcac tggtcaatac gcctgcgccg acataaggca tattcaatgt
     4381 ctctaagaag ccttggatcg ttccatcttc cccgtttggc ccatgtaaaa ctggaaaaac
     4441 gatggctcct tcttctttga tttcgcccgg attgatcact tttcctgtaa agccttcctc
     4501 tgtctgtcca cttgggtccc atgaaagatg caagacatct ttgctggcag gtttttctgt
     4561 tagtaatgga cctttgaccc attgtccttc ttttgtaata aatacgagtt gaacttggta
     4621 ataattataa taaatggcat ttaaaactga aaatgcggaa agaatggaca cttcatgctc
     4681 tgcgctgcgt ccgccatata gtaaagtaat cttcaaaaga ttgtcctcct gtattctaaa
     4741 tcgttcagtg atttactctc atattttagc acaaaacaaa aataaacgaa taacttattt
     4801 ataaaaaaac gagccagctt ctaaaagaac gagcgtcatt tattttttgt cagctagatt
     4861 atttgtcccg ttttttataa aaagttatga taaagaaaag gagggatgat gatggcgcaa
     4921 acaaatccac gcagccgctt ggcagaaatc attcgagtgt tcatcaatta caatgtagtg
     4981 ccaaactttg tacagcaaaa aaatcccgaa caggtaaaaa aagcctttga agaactaggc
     5041 cccaccttca tcaagatagg acagatgctt tctgttcgag aagatttgct ttcttctgcc
     5101 ttcactcaaa cattcaaaac attacaagat agtgtaccta gcgacacatt ttcaacggtc
     5161 aaaaagacga tcgaaacaga actttctctt tcattatcag atatattcga tgatttttca
     5221 aaatcgcctt ttgcctctgc ttctatgggg caggctcatc gtgccaagct gaagaacggt
     5281 gacgcggtag tcgttaagat ccaacaccct aacatcgccg aagagatccg acttgatctt
     5341 caactatttg aacgagcgat ccctttgatc aaatacattc ctgaaactag cgtagttgat
     5401 ctaaaagggg tactccaaga agtaaaacgt tccttgatca atgaaatgga cttcttaaaa
     5461 gaaagtcaaa atggcgaaca attctatcaa aagaacaatg gctggaaaga gatccgcagc
     5521 cccaagattt atgacgcttt ttgttcgaaa aaagtgatcg tgatggaaga aatgtctggt
     5581 aaaaacttga atcatttaat gaacgccgaa aacaaaactg aaacttttat cactggaatt
     5641 caaaacaagc agctgaaaca agaagttgca aagcttttag ttgagaactt tatgaaacag
     5701 gtgtttgatg atggtttttt ccatgctgat cctcatccag ggaatctgct attccatgta
     5761 ctaacaaaag aagaacagac acaagctagc cgaaaaactg aaacagtcca tgaaaaagag
     5821 ttcggttcct ttgcttttcg agcctccact tctgcagaag accctgttgc gccatatacc
     5881 atcaactaca tcgattttgg tatgatgggg catctttctg caggacttag gcaaaaactc
     5941 acacaagccg ttttagcgct gtatacgaaa gatgcttatc gaatcgaaaa agcggtcctt
     6001 cgactttgtc agcaagaagg ctcgtttgat gaaagtcggt tccaccaaga actgacaagt
     6061 tttcttgaac agtattatga ttctcctatt gatgaaatca acttgcaaga agtattcaca
     6121 catgtcgtga cgatttgtca tcaaaataat ctgcagttcg atcgtgatat cacgcttttg
     6181 ctgaaagcat ttgggacatt agaaggggtg atccgcgtcc tcgatcctga agtttccctc
     6241 atggaagtcg cttcgccttt tgctcaacat tattttctca ctcatctgga tgtagaagat
     6301 acattgaaac aaagcggatt agacttattg gaagggatga aggctgcgcc taagatccct
     6361 caacagctcc accatctatt agaaatgtgg acttcgggac aaggaaaagt caatttggaa
     6421 ttgaaaaagc aagacaaatt attgagcagg atcgaaagta tgatcaatcg gcttgttttc
     6481 ggtatgattt tagctgcatt gatcgtaggg tcctccctgc ttgtccaagc agcacctgta
     6541 gaaaacgcag aggtcgtttc cttattaggg atatttacgt atgctattgc cgcattcgtc
     6601 attatttttt tggcaatcga tgcattgatc caaatgtata agaagaggaa aaagtagaaa
     6661 aaggttgtga cagaaagaat cagcttcaag aaataagaag gggttttaca taaattgttt
     6721 ctcaaatttt tgtgaaaatt cggcttattt ttcgaagagg ctacttctga aacaccgaga
     6781 ataaaaaatg tgaaaaagct attctgcacc cagtaataag aacaatcaaa aagggaacat
     6841 ggaacacatt tatcctgttt ccctgttccc tttttgatgt attcgagcat aaataaaata
     6901 ccttcgttac ctactatctt ctgtacttgg taaaccttag tcaattgcct tttttaacgc
     6961 ttgacgaaaa gacatatgat ctttctccgt aaagggtttt ggaccctttg tccttatgcc
     7021 agcttttctt aactttccac tatgataacg ttgttctaga agcgtgccga tattcgcttc
     7081 gtcaaattct tttccagagt gatggaacac tcgcacaccc ttcggcaata caagtgaagc
     7141 taaaccataa tcttgcgtga tcaaaaagtc attcttctta accataccga cgattttata
     7201 atccgcacta tcagctccgc ggtctacgta gacgatttgg acatgattag cgtattcttt
     7261 ggtcgtataa tggtccacac tggtcacgat aaccacgtca agaccatagg attctcctaa
     7321 ttttatgaca tcttctttta ctggagaacc atctccatca atgaatagcc tcattctttt
     7381 tcgccttctt ccataaatga taaacagtta ctaatccgct cagtaaaaga ccaacattga
     7441 tccattttgc caagataagc agttgtacac tttctatcgg cactgattca tggttcagcg
     7501 tgatcaataa tagatggatt ggctctccca tttcttttgc taaaacaaac aaaagcgtca
     7561 tcaataaaaa ctggtggatc acactccctt tattttgagt agtatacttg acaacgatcc
     7621 ggcgatccat tggaatagct gtatcagttt gataatattc cttatttagc cagctgcctg
     7681 ttactacgtg ccagaaacta agcagaaaga cacctatcgc aaaaccattt ggaatcaaga
     7741 ggaccaataa agaagaactc agtccaagca attgcatagc caatggagac catgttaaac
     7801 gtttcaaaat ccatggtccg actatttttg ctgcaatcaa gcctgctgca taaggcaaat
     7861 acataaaaat cgaaagtttt tctaatccgt acagtatgcc aacataaaaa ctgctaaata
     7921 aaaaaaggaa attccctagc attccattta gaaaagtaaa cagatttagc cagcttggca
     7981 acttccattc cttcttctgg tgtcctaaaa caaacacgaa caagatgaac agcagcaaga
     8041 aaccataaat agcaagatca aaaagccttt catcgaataa caatcgggca tttctcaaaa
     8101 gaaacaataa gccaaagaat atcaaaaata aaacaagctc tcgcgtagct accagatttc
     8161 ttttgatgtc tttgaaatcc agttcgtaac gcggataatg actaaccgta tggtaggcca
     8221 tgacgtaaaa aagtgtataa atagtaaagc ttgctgcaat ctgagtcggt gcaggcaact
     8281 caatcgcttg atacaggaga aatagccaga tagctgcaaa taagtacttt ttcccatcca
     8341 tattgataaa accttgcgtc ttttcgtgaa aattgaccgt ggtattagca ggtacaagcc
     8401 atgctgcact cagccctagc aaaacacttg aaaaataaaa taaaggaaaa taaaatacgc
     8461 ctaatattcc aagcaatgaa ccagcaccac caatcagcag agcgatcatc agcaacgtgt
     8521 aactatctag accaaatcgt agactgttga ctaaaaatag tccagtcatc cgaaaggcat
     8581 aaaacagcgt aaatggcaaa atcgtcagcc aattatgcga gtctgttagt cccaaaaata
     8641 aaagataagg aataaaaata accgtgttac ttaagataaa gggcgttgtt tgtgtaaaat
     8701 tacgcccaaa tcgtttgagt ttttcgaaca tgacaatggc tccttaaaca gtcttataac
     8761 taatctgctc ttcgatttta gcattaaagg ggaaataaac caattttaca gaatatattc
     8821 ttgtaaaaaa aaatacagaa agaagcataa gcttctttct gcatttttac ggtacttgat
     8881 tactcaagca gacaagccct tctccacaaa agtcttgccc gacacccctg catccggcag
     8941 gtgataaaga tagaaatcaa ttatcctgtg cctccaaaat caattaggag ctctgtaaac
     9001 cagagtcctt ctcgacccgt cgcataaatt tactttaaca cagttcctat gtttatgcga
     9061 gagctttttt cattttataa tcgaccattt gaaatccttg accgatatct gaaataagtt
     9121 cttctgtaat ctcaaatcct tcatgcttat aaacctcgat tgcttgatga ttcccctgat
     9181 tcactcgcaa aaagatctct ctttcctttg tttgcgcagc aagttcttca taccagcgaa
     9241 aaactgaaga agtcaatcct tgaccacgta ctttatttag caaatataat ttacttaaat
     9301 aaatttttcc atttatctct tcataagcag tgtaaccgac agtcttttct tcgcttttta
     9361 ataaaaagta ggacacacct tctgtcagtt ctttttgaat ctgttttttt gactgataag
     9421 ttttcagcat gtaatccact tgtttttgac caatgatcgg cacaaaagct tccggccata
     9481 cttcttgtat gactgccatc agctcatcca gttcattttc tgttactttt attttttcca
     9541 tacacccacg tcctttttta cattataaat gaccttgcgc ttgatggaat aaaaagagca
     9601 tgatcatgct atacacaccc accgaccatg gtttcaagaa aatcacaatc tgcataaatt
     9661 ccctaaaaga taatttcgtc aaaccagcta ctagacagat caagtcgtct ggcgcaaagg
     9721 gaagcagcaa ggtaacaact agtacttttt taatatcttt cgtttttctt gtcagcaatt
     9781 catcaaattt ctgatacttt tttggcgata aaataagctg gacaaaggct acgccatagt
     9841 aacggacaag caaaaatccg attgcttccc caatcactag gccaatacaa ctatataaaa
     9901 gcccaaatcc attgccaaac atcagcatgc ccgctactga ggagatccct cccggcaaaa
     9961 taggaatgat cacttggatc acttgcaaca gaacaaatat catcactgca tacttgccaa
    10021 attgctggat aaacgcttgc aaagacgtgc tcgatcgaaa aatcccatga tgataagcat
    10081 aaagaatcaa cacaaagaaa aaaaacagac caataatggg catgatctga agacattttt
    10141 gtaaaactga tcctttttta gacatctata ttccccacat cttttttcat ttattaaaac
    10201 tgcctcct
//
LOCUS       DACEXQ010000083         9941 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.083, whole genome shotgun sequence.
ACCESSION   DACEXQ010000083 DACEXQ010000000
VERSION     DACEXQ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9941
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.083"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..205
                     /locus_tag="IXH04_001484"
     CDS             <1..205
                     /locus_tag="IXH04_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852096.1"
                     /translation="IADIKLIKEVLQESTANAIASGSGISLSTAKKLKSGERNVEKLN
                     LSDAIKITEFAIKNRHVKIEIWK"
     gene            complement(291..1202)
                     /locus_tag="IXH04_001485"
     CDS             complement(291..1202)
                     /locus_tag="IXH04_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1852097.1"
                     /translation="MKIKIERNQKEKSPKKNKERVISVGKHRKMVLALWLLLSCSLAF
                     GIYRNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWRNNKEAIEKRMSNL
                     EQYLTENGLALSQDMVRADIPTSSEVQSIKILDVEKHSEEFIVSFLVEQQITEGKKTQ
                     SISSAYRVTIFEDENGSHIVTSLPTMIAKPEKAKYESKQIGIESDIDAKTTEEVTDFL
                     ETFFKLYPSASEKELEYYVENKAVQPINSNLKLVELVNPTFKLKGKEIQGEIFVKYLN
                     NTTKVSNIFEFTLTLQKVKNWKIIDIG"
     gene            complement(1221..2225)
                     /locus_tag="IXH04_001486"
     CDS             complement(1221..2225)
                     /locus_tag="IXH04_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ1852098.1"
                     /translation="MNVKKLSIISLTILTLSFMVVLMCVGLLFGEDSEGSNSSGASSG
                     GSNVSEEVLKHRAMVEKYAKESNISEYVLILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVAKNGKKHNFALA
                     ESFSKEKSSGQKVDYPNPIAIKKNGGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGASPETGFDCSGLTQWCYAKVGLKLPRVAQDQYNAMTHIDVKDA
                     KPGDLVFFHSTYDTGTYVTHVGIYAGENRMFHAGDPVGWTNLTESYWQQHLIGAGRYK
                     "
     gene            complement(2222..4345)
                     /locus_tag="IXH04_001487"
     CDS             complement(2222..4345)
                     /locus_tag="IXH04_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ1852099.1"
                     /translation="MKKKLLRIVGIIAISLCTILLLLSLIGTVAEATGLVDDTVKSGN
                     LYSQYSLDNYQLDFFVDSSWGWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQVSGFYFGFLLLMILALGVYVAYI
                     GLLKREATKAVRAVINFVTIFLLSGSFIAYAPTYITKINNFSSDVSEAALSLGTELVV
                     PNSESKGKDSVDLIRDSLFSIQVRQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRIMRKPHMLMNRKLR
                     QLNRNVIRTLAFGGGAAVGHKLEKEQSKSKFKPSGSSLRKNSRLPNDHEVPSDSTKEN
                     PVSNNKKQSRMNLAGRKMGKVLDTQALVKDKTKQVRDQVRNTPTNLKYNFHKGIEKTK
                     KAPKEFKRGLVQEKANRAELREKQRQHRDEKMAEKRKVLNETGNRHKKRRENVSIKPE
                     RQPQKDRAPKKEAPKQIVRVNPNPEIKRKLSSHEIVSKRISQPSSKKNSFQQVKQRST
                     LPKRTNQKVQKRMNPRPKSRSGEKK"
     gene            complement(4350..5630)
                     /locus_tag="IXH04_001488"
     CDS             complement(4350..5630)
                     /locus_tag="IXH04_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ1852100.1"
                     /translation="MHVRCGAGENLEITSKGYLSLFTSDFLAGLGFGATQMLGELEGI
                     YFGYNVDTGRNVYLKPALASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFG
                     GQAVIVDPKGERGSWKETLPEIASEINILNLTSKPENQGLLDPYVIMKKKKDSESLAI
                     DILTFLTGISSRDGEKFPVLRKAIRTVTQSNQRGLLQVITELRNDGTPVAENIADHIE
                     SFTDYDFAHLLFSDGSITQSISLEKQLNIIQVADLVLPDAETTFEEYTTMELLSVSML
                     IVISTFALDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQ
                     NSDDLLDERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDQEDESNQKRLRDLENGQCLM
                     QDLYGRVGVVQVHPVFEELFHAFDTRPPVQKRME"
     gene            complement(5670..7472)
                     /gene="ltrA"
                     /locus_tag="IXH04_001489"
     CDS             complement(5670..7472)
                     /gene="ltrA"
                     /locus_tag="IXH04_001489"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ1852101.1"
                     /translation="MSTELRYYEYYQMQETFDWLYERSSKQATKGIDLYKIIMSEENI
                     LLAYRIIKSNTGSKTSGIDNQTISDFKFQNKQKFVNDIRKTIENYQPQPVRRVNIPKS
                     NGKTRPLGIPTMKDRLIQQMFKQVLEPICEAKFFKHSYGFRPNRSTEHAIARCNFIAH
                     KCKCHHVVDVDIKGFFDNVHHGKLIKQLYTIGIKDRRVLAIISKMLKVTVAGQGTSTS
                     GVPQGGVLSTLLSNVVLNDLDWWIANQWETFQTKRKYSSVDNMHHALVKSKLKKMHIV
                     RYCDDFKIFTTNHKHAVKIYHAVKGYIKQRLNLDISPDKSKITNLRRNYSEFLGFELK
                     VIKQKKQQYVTVSRVSKKSKSRIKKEIREKIKAIQKSPTRKTVNQYNQYVRGIQNYYQ
                     KATLINHDFSSIYYSCLPALYNRLKPFGKYEIPRSPPITYKKFYPLTKRTFCVRKNYL
                     FPLDSVKWKRLEFFKPHINNYTQDGRLAKLRKLKPSVYSELVTLSTMETDESLEYLDN
                     RLSRYSMQNGKCSVTGIFLRTQDVHCHHIIPRKFGGTDEFDNLVIIHKWVHRLIHAKT
                     KETIKEYLQLLQLTNKQMKKLNKYRLECNLTEFH"
     gene            complement(8180..9409)
                     /locus_tag="IXH04_001490"
                     /pseudo
     CDS             complement(8180..9409)
                     /locus_tag="IXH04_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
     gene            complement(9396..9785)
                     /locus_tag="IXH04_001491"
     CDS             complement(9396..9785)
                     /locus_tag="IXH04_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1852102.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHMPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
BASE COUNT         2843 a         2040 c         1676 g         3382 t
ORIGIN      
        1 aatagctgat attaaattaa tcaaagaagt tttacaagaa tctaccgcaa acgcgatagc
       61 atctggatca gggattagct taagtacagc aaaaaaacta aaaagtggcg agagaaatgt
      121 agaaaaacta aatctttctg atgcaataaa aatcactgag tttgctatta agaataggca
      181 tgtgaaaatt gaaatttgga aatgacggat acaactatta tagttttgtt ttttctaagt
      241 atcatattta tgaatatgct tttgctaaac atctaattat agattaattt ttatccaata
      301 tctataattt tccaattttt taccttttgg agagtaaggg taaattcaaa gatattgctt
      361 actttagttg tattattaag atacttaaca aaaatttctc cttgaatttc ttttcctttc
      421 agtttaaaag taggattaac caattcaact aatttcaaat tactattaat tggctgaact
      481 gctttgtttt caacatagta ttcaagttcc ttttccgatg ctgatggata cagtttaaaa
      541 aaggtctcta aaaaatcggt gacttcttcc gtggttttag catcaatgtc agattcgatt
      601 cctatttgtt ttgattcata ttttgccttt tctggctttg caatcatagt aggcaaactc
      661 gttactatat ggctcccatt ttcatcttca aagattgtca ctcgatatgc agaagaaatt
      721 gattgtgtct tttttccctc tgtaatctgt tgctcgacta aaaatgaaac gataaattct
      781 tccgagtgtt tttcaacatc tagaattttt atagattgaa cttcggaact cgtgggaata
      841 tctgctcgaa ccatatcttg gcttaaagcg agaccgtttt ctgtaaggta ctgttcgaga
      901 ttactcattc tcttttcaat tgcttccttg ttatttctcc atgaaaaata ttctttcgca
      961 aaatcctctg taaaactttc aacgcctgaa gtattcacta ccttctcttt tataactact
     1021 ttttcatgag tcgtatgctg atcaatggct gtgaaatttc tgtagattcc aaaagcaagg
     1081 ctacaagaca ataaaagcca aagagcaagc accatttttc gatgcttgcc cactgaaatg
     1141 actcgttctt tattcttttt tgggctcttt tccttttgat tccgttcaat ttttatcttc
     1201 atgttttttc ctcttttctc ttatttgtac cgtccagcac cgataagatg ctgctgccaa
     1261 tagctttctg ttagatttgt ccagcccact ggatctcctg catgaaacat gcgattctct
     1321 cctgcataaa taccgacatg ggtcacataa gtaccggtat cataagtcga atgaaagaaa
     1381 accaaatcac ctggtttggc atcttttaca tcaatatggg tcatggcatt atactgatct
     1441 tgagccaccc gaggaagttt cagaccaact ttggcataac accattgagt caagccacta
     1501 caatcaaacc cagtttcggg acttgctcca ccaaacacat aagccgttcc ttcatactta
     1561 aacgcctcat caaaaatccc ttggactgtt ttatcatcaa actgtgttgt agttaggtat
     1621 tgcttcacta ataagcaata aaacatattg ccatagttat accgccaccc accatttttc
     1681 ttaatcgcta tgggattagg gtaatcaact ttttggccgc tcgatttttc ctttgaaaaa
     1741 gattcggcta aagcaaaatt atgcttcttc ccgtttttcg cgacatgatc cagatacccg
     1801 ccaccatagt tatacgcttg aataatgcta tcttgatcac accctagttc tttggaacgc
     1861 ttaaccaaag acgcaaaata gagacatcct tgtttgatgg atgcctcggt acttaatgaa
     1921 ttcggtggca acccggctga ctccgaactc tgcatcacat cttccattgt gccaccagat
     1981 tccacttgaa taatcgccaa aagaatcagg acatattctg agatgtttga ttccttggca
     2041 tatttttcca ccattgctcg gtgtttcaaa acttcttctg agacattact tcccccagaa
     2101 gaagcacctg aactattact tccttcactg tcttccccaa acaacaagcc cacacacatc
     2161 aagacgacca taaaggagag tgtcaagatg gttaacgaaa tgatacttaa tttctttaca
     2221 ttcatttttt ctcacccgac cttgattttg gacgagggtt cattcttttt tgtacttttt
     2281 gattggttcg cttgggcaga gtagacctct gtttcacttg ttggaaagaa tttttcttac
     2341 ttgatggttg acttattctt tttgaaacaa tttcatgact agatagtttt cgtttgattt
     2401 cgggattcgg atttactctc acaatctgtt ttggtgcttc tttttttggc gctctatctt
     2461 tttgaggttg gcgctctggt tttatcgaaa cattttccct tcttttctta tgacgattac
     2521 cggtttcgtt caatactttg cgcttttcag ccattttttc atctctatgt tgtcgttgtt
     2581 tttctctcaa ctccgctcga tttgcttttt cttggacaag tccgcgcttg aattctttcg
     2641 gtgctttttt cgtcttttca attcctttgt ggaagttgta tttcaagttg gtcggcgtat
     2701 tccttacctg atcccttact tgtttagtct tatctttcac caaagcttgg gtatccagaa
     2761 ctttgcccat ttttctccca gccaaattca ttcgggattg tttcttatta tttgaaactg
     2821 gattctcctt agtcgagtca gatggtacct cgtgatcgtt aggtaatcgt gaattctttc
     2881 gtagagaaga accggatggc ttaaatttgg attttgattg ttccttttcc aacttatgtc
     2941 caactgctgc accaccgcca aaagccagag ttcggatcac atttcgattg agttgccgca
     3001 atttacggtt catcaacatg tgcggtttcc gcatgatgcg ccttcccatt gactgactat
     3061 cattgctttg gagattgaac atactcatga tgtcacctaa tttgaaatag ataccggcaa
     3121 aggtcacgat ttgcaggaac gcaaccataa agaaaggata ggtagctgaa atattgaaaa
     3181 acatggtgga gatactaaaa gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     3241 tcgtattgaa taaacgaata atcgccttct ttcccaagct ctcataagtt ggtaacatgg
     3301 ataacagaaa actaattggc agaaacatgg cgaaaatgat aaagagaatt tgggagaaca
     3361 acataatccc cgtcagtaag aagacaaaga atgagatacc aatattaaac aacaccaaaa
     3421 agaccacggt ccctagtcgg ttcatggtct ttgtaatact tagattcgcg ttgtcattgt
     3481 cttcaatttc agcttttacc gcttcttctc gatcctttcc cttattttca tctggactca
     3541 cagataaaat tttattgacc cggtcttcac caatttcttc tatgttggaa tcatcaaatt
     3601 gcaacaagag ccaaggttgt cggacttgaa tagaaaacaa actatcacga atcaaatcca
     3661 cactgtcttt tcctttgctt tccgaattcg gcacaacgag ctctgttccg agactaagag
     3721 ccgcttcact aacatccgaa ctaaagttat tgatttttgt gatataggta ggcgcatacg
     3781 cgataaagga accagagaga aggaagatcg ttacaaagtt aatgacagcc cggactgctt
     3841 tggtagcttc tcgctttaat agtccgatat acgccacata gactcctaga gctaaaatca
     3901 ttaataatag aaatccaaaa tagaaacccg agacctgtaa tccattttca gtaataccgg
     3961 ctagcgtttg gatatttttc ccaatacttt ctgccgtatc cgaaataaaa tctaatttgt
     4021 acgcttcttg taccacatac ccggtcgcat ttgataaata caaactaacg gtccaaatga
     4081 aatttgtaat ggcgtagaga ccgtacatca cactctttcc taacccatcg ccccagttcc
     4141 aaggcagcca gccccacgaa ctatctacaa agaaatccaa ttgatagtta tcaagtgaat
     4201 attgagaata gagattgcca gatttaacgg tatcatcaac taatccagtt gcttccgcta
     4261 ctgttccaat cagactcaaa agaagtaaaa tggtgcagag actaattgca attattccca
     4321 caatccgtaa aagtttcttt ttcatctcct cactccattc tcttttgaac aggcgggcgt
     4381 gtatcaaaag catggaagag ttcttcaaag actggatgca cttgtaccac accaacgcga
     4441 ccatacaagt cctgcatcaa acattgccca ttttccaaat ctcgcagacg cttttgattg
     4501 ctttcatctt cttgatcaag accaaagaat tcgagtgtct tcttgatttc atgaatatct
     4561 gtgcttcgaa aggcaaattt cagcccaatg ttgtttttca gacgttcatc taataaatca
     4621 tcactatttt gagtgacaaa gtaaacgccg gcattcatag cacggccagc acgaactagt
     4681 ttattcgaaa gagtttttcc ttgtgcgact tgaaggaagc tccaggcttc atccaaatcc
     4741 acgattttga aaatttctcg attgctatga ataaaatcca aggcaaatgt ggagataaca
     4801 atcaacatgg aaacactaag caattccatc gtagtgtact cttcaaatgt agtctccgca
     4861 tccggcaaaa ccaaatctgc cacctgaatg atattcaatt gtttctctaa actaatcgat
     4921 tgtgtaattg aaccatctga aaaaagtaaa tgcgcaaaat cataatcggt aaacgattca
     4981 atatgatcag caatattttc cgcaactggt gtcccatcat ttcgaagctc ggtaatgact
     5041 tgtaacaacc cacgttgatt gctttgggta acggttcgaa tagcttttcg taaaacggga
     5101 aatttctctc catcccgact agaaattcca gttagaaaag tcagaatatc aattgctaga
     5161 ctttcagaat ccttcttttt cttcatgatc acatagggat caagtaaccc ttgattttct
     5221 ggtttactgg ttaggttgag gatattaatt tcactcgcaa tttctggtaa ggtttctttc
     5281 cagctaccac gctctccttt gggatcaaca atcaccgctt gaccgccaaa caggacagca
     5341 tagtaaacta aaagattatt actaaaggat ttcccaccac caagtgaacc aagaaaagcg
     5401 gcggccagtg cattggtgac ggaacctttg accccttgtg aagccaatgc cggtttaaga
     5461 taaacattgc gtccagtatc cacattgtag ccaaaataaa ttccctccaa ttctcctaac
     5521 atttgggttg ccccaaagcc aagcccagca agaaaatcag aagtaaatag cgataggtaa
     5581 ccctttgaag ttatctctag gttttccccc gctccacacc gtacgtgcaa ctttcatcgc
     5641 atacggcgtt ccaacttgtt cgtatttctt tagtgaaatt cagttaaatt acattctaaa
     5701 cgatatttgt ttaacttctt catttgctta tttgttagtt gaagaagttg tagatactct
     5761 ttaatcgttt cttttgtttt ggcatggatg agtctgtgta cccatttatg gataataact
     5821 aggttatcaa actcatctgt acccccaaat ttacgaggaa taatatgatg acaatgaaca
     5881 tcttgcgttc ttagaaaaat ccctgtaacg gaacattttc cattttgcat agaatatctt
     5941 gacagtcgat tgtctaagta ttcaagactt tcatctgttt ccatcgtact aagggtaacc
     6001 aattcagaat aaaccgaagg ttttaatttc ctcagtttag ccaatcgtcc atcttgcgta
     6061 tagttgttga tgtgaggttt gaaaaattct aaccttttcc atttcacgct atctaatgga
     6121 aataaataat tcttcctaac acaaaacgtt ctttttgtga gaggataaaa ctttttatac
     6181 gtaatgggtg ggcttctagg gatttcatat tttccgaatg gttttaaacg attatatagt
     6241 gctggtaagc aggaatagta gatagaagaa aagtcatggt taatcaaggt ggctttttga
     6301 taataattct gaatacctcg tacatactgg ttgtattggt taacagtttt tctagtagga
     6361 gatttctgaa ttgcttttat cttttctctg atttccttct taattctact ttttgatttc
     6421 tttgagactc tcgaaacagt aacatattgt tgtttctttt gttttatgac ctttagctcg
     6481 aatcctagaa attctgaata atttcttcta agatttgtaa tttttgattt gtctggtgaa
     6541 atatctaggt tcaaccgttg tttaatatat cccttgaccg catgatagat ttttactgca
     6601 tgtttatgat ttgttgtaaa gattttaaaa tcatcacaat aacgtacgat gtgcattttc
     6661 tttaatttcg actttaccaa agcatggtgc atattatcta cactgctata ttttcttttt
     6721 gtttgaaatg tttcccattg attggcaatc caccaatcta agtcatttag aacaacattc
     6781 gataataatg tagataacac gccaccttgt gggacgccag aagtggaagt gccttgtcct
     6841 gctactgtca cttttaacat tttagagatt attgctaata cgcgtcgatc ttttataccg
     6901 atggtataaa gctgtttgat taactttcca tgatggacgt tatcaaagaa tcccttaatg
     6961 tcgacatcta ccacatgatg acatttgcat ttatgtgcaa taaaattaca tcttgctatt
     7021 gcatgttctg tagagcgatt aggtcggaac ccataggaat gtttgaaaaa ttttgcttca
     7081 catatgggtt ctaacacttg tttaaacatt tgttgtatca agcggtcttt catagtggga
     7141 atgcctagag gacgagtttt cccattactt tttggaatgt tgactcgtct aactggctgc
     7201 ggctgataat tttcaattgt ttttcgaata tcatttacaa acttttgttt gttttgaaat
     7261 ttgaaatcac tgattgtttg attatctatc ccagatgttt tagaaccagt atttgattta
     7321 ataattctat atgctagtag gatattttcc tctgacataa ttattttata aaggtcaata
     7381 ccttttgttg cttgtttgct acttctttca tagagccaat caaaagtttc ttgcatttga
     7441 tagtattcat aatatcgtaa ttctgtactc actgatggat tatttctcct aactttaaaa
     7501 ttagtcccat cctcttacca gatcttgtga gtttcattta actgtattca cttttcaaag
     7561 aacaagtctt ggggctatcc ctccacgttg attagacgct tcacaggtac tgtgccccta
     7621 ctctcgcaga aattaaggat tttcgactat tagtcttata tccaacgctc caataacagt
     7681 tgttgaataa gtgttttctg ttttcctcgt tccagttatt ctagccatac atctatcctt
     7741 aggtgcttac tttgagcctg aatattatat aaggtcattg ttccttatcc cgaatttcat
     7801 aaatacgact cttacttttc ggtaatattc gttgcttttg caacccaatt atctttcgat
     7861 aaaagtcatc ctttagaccc gtacattcgc aagttcgtca gttccgtagg aacattctca
     7921 ccatagatag tttgtggcgt cccggcatat cctttaccat ataagttatt acttacttag
     7981 gtattgttct gccgacttta cctagcttca cacgaatatt cactgttatg aatattcgca
     8041 tgtaggagta tcagaccagt tgcttcaacc aacttgacgg ttttcatttc aactgtgaaa
     8101 taactagttg taattatctt tagttcttga taattctccc atttcatgcc tatgcactta
     8161 tagggaaacg agtcgcacca tattggatat agtcattcat gtatcgcttg ctagctggca
     8221 aaaactcttc atgaagtccc atcatatcgc caaagggacg aaccaatttt acattggtat
     8281 catcataaaa atctaaaacc tcatcacacc gacgttttaa ttcatccaat gattctgcac
     8341 tcactcgaac aacatagctt aatttgtaca tggattcttt ggattgatcc aacgttgttt
     8401 ctagttcatc cacggagtcc aacgcatcca agacattgga attggtttca ttgtcggact
     8461 ggtacgcatg gttgtctaag tctttcagtt cttttttctt attgcgaact gtcgccagtg
     8521 cctttttatt cgtcacgatt tctacattca tactcgtgtc gattgggaaa gtgaattgtt
     8581 gttgctggta ataaaagagt tcagaagatg gaaactccat ctcaccgaca atcgtattaa
     8641 tcgttagata ggccacataa gattcatgat tttcatgttc catgtgtaag taccgaggct
     8701 tctcttcaat caaacagcga cttgggcgaa gtaggtcata gcgctttatc aatgtttcag
     8761 attttagctt tttcttagac aactgaaatt catattcttc aaatggtgtt cctttttctc
     8821 cataaatgtg ttcaatcaaa taagttagat cactaggtgt tactcgtctt actttgaatc
     8881 tacgagccaa tttactttcc atcaattttt caagcttagt ataccggcga atttcttcat
     8941 tggataacga aacaaaatca cccatcaaat gatgattcac tccatagaca aactcttgaa
     9001 gtccggataa aaaagagttc ttgagacttt tcaggtttac ttcttcgtct gtgacaatca
     9061 gtttgaagcc aataaaaaag cgatagtcaa tttgactatc gccaatcatc gataccaatg
     9121 catccgtctg taagtcgatt ctctgttttg cgacttcttt caaacgacca gttatctctt
     9181 ttttcgaccg ttcctgcgtg gcacgaatgc tgctttctgt cgcaatttgt aacgcatgaa
     9241 tcttaccttc acgattttgc gcaatcaatt ggcgaaagtt atcgtggact tgatacttct
     9301 gttcaagtga taagaaggag taattgtaag ggactagttc gtagtaagca aagcactctc
     9361 cctcttggtt aaaaacaaga ttattttcaa tatatttaat cgggtacata attcacactc
     9421 ctcactaggg tgattggttc atcccatcgt tttttctgtt ctttgacttt ctttcctgca
     9481 aaagtaacct taaatcgcaa gtgataacta ataaaggatc gtaaaaaccc taatggcttc
     9541 tttccatcaa aggtcttttg cgacataaac caagtgactg ccacaggtat gcccaagtat
     9601 tttaaaaatg ccccctctat catagatagt ggaggcatat gagcaaacac aatgacaaac
     9661 agcaaggata agacaaacca agccatttga ttaaaggtta caggaaaagg cagctgaaag
     9721 tcattgattg cgtaaatgac tttttcaact gcccagatac tggtatagct ttttattttt
     9781 ttcattagtt tcacttccta aaaaagcgcc tgataaagac gactctgcat gaatattaat
     9841 tttcttttta aaagtgtatt ccaactttca atctatcaac tgaggtttat cagaccataa
     9901 tctgtggatt tcttggtatc cagctgaagt aataaaagta t
//
LOCUS       DACEXQ010000084         9930 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.084, whole genome shotgun sequence.
ACCESSION   DACEXQ010000084 DACEXQ010000000
VERSION     DACEXQ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9930
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.084"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(461..1090)
                     /gene="udk"
                     /locus_tag="IXH04_001492"
     CDS             complement(461..1090)
                     /gene="udk"
                     /locus_tag="IXH04_001492"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAQ1852103.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1174..1869)
                     /locus_tag="IXH04_001493"
     CDS             complement(1174..1869)
                     /locus_tag="IXH04_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAQ1852104.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1882..2598)
                     /locus_tag="IXH04_001494"
     CDS             complement(1882..2598)
                     /locus_tag="IXH04_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAQ1852105.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2928..3533
                     /locus_tag="IXH04_001495"
     CDS             2928..3533
                     /locus_tag="IXH04_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAQ1852106.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            3858..4181
                     /locus_tag="IXH04_001496"
     CDS             3858..4181
                     /locus_tag="IXH04_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAQ1852107.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4363..5733
                     /locus_tag="IXH04_001497"
     CDS             4363..5733
                     /locus_tag="IXH04_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAQ1852108.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5863..7164)
                     /locus_tag="IXH04_001498"
     CDS             complement(5863..7164)
                     /locus_tag="IXH04_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ1852109.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(7394..9385)
                     /locus_tag="IXH04_001499"
     CDS             complement(7394..9385)
                     /locus_tag="IXH04_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAQ1852110.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9529..9795)
                     /locus_tag="IXH04_001500"
     CDS             complement(9529..9795)
                     /locus_tag="IXH04_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ1852111.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
BASE COUNT         2907 a         2063 c         1799 g         3161 t
ORIGIN      
        1 tagggggtgc gacaaaagag cttcttcaaa aaaataagtc gaaatctcac acaaattcga
       61 gaaataattt tcgtaagttt cttcttattt attgcatctg accacttctg ttacaacctc
      121 tcgttaaacg gtgttcaaaa gcttgcatct acgataataa gcccaaccca acgaaaaacg
      181 tgaggagctg ttttatttgg atgctcttat cgcttgatgc aaagacagct tttttacaac
      241 ctctacttca cggtgctcaa tcagctgacc ttcggtatta gctcaaaaaa cggtaaaata
      301 taaacagcta ttttccgttt cttcttttta atacttaggt caagatgctg ttttcacaac
      361 ctcttgttat aaataaaaaa acagttggct ggtgactgtt caaaaagcag aatccttgct
      421 tttcaaacgg tcgcagtccg ttcaactgtt tcgctgcgca ctatttatgg tttaagaaac
      481 tagctacttt tgttgtaatc aaatcaatag caacgtggtt ttctccgcct tctggaacaa
      541 tgatatccgc atagcgcttc gttggttcga taaattgatg atacatcggt ttaacaactg
      601 tcaaatattg ttcgatcacg gagtccaatg tgcgtccccg ttcttccata tcccgtttga
      661 tacgacgaat gatacgaatg tcatcatctg tatctacgta tactttgata tccatcaagt
      721 cacgcagacg ttcgtcttct aaaatcagga ttccttctag aatgatcact tcttttggtt
      781 cctgaatgat cgttgcctga cttcttgtat gcgctacata gtcatacacg ggtttttcaa
      841 tggctttata gttcagtaag tcgcctacat gctgaatcag cagatcatta tcaaaggcaa
      901 atgggtgatc gtaatttgtg ttcaatcgtt cttcaaaact caaatggctc tgatccttgt
      961 aataagaatc ttgttcaagc atcatgatgg aatggtccgg gaagttgttg aaaatcgcac
     1021 ggctgacact tgtctttccg cttccggatc cacccgtcac accaatgata attggcttgc
     1081 tttttgtcat tgactaaaac ctctttcgta tccgtttttt cattctaatc tattatagcg
     1141 aaaaaacacg aaaatgctat atttttctga aaattaatcc ttttcaattt ccaataatga
     1201 ataaagttcc tctaatgaat cgattcgata agtaggctgg atttcttcga tttctggtaa
     1261 tttatttcta ttgaaccaaa tcgtatcgat ccctgccata tttcctcctt tgatatcaga
     1321 agtcaaagaa tcaccaatga tcacagtttg ctttttatca aaattaggaa tgtgggaaaa
     1381 gacatgatca aaaaattctt tcatcggctt ttgcgctccg acagcttctg agacaaagat
     1441 atccttgaag taatcaagca ttcctgcatc tgttaatcgc cgatgttgcg tgtgcgacac
     1501 accgttcgtc acaatataaa gatcagcatg cggatggacc tttttcagga tttctagact
     1561 tcctggaaca agatcatgac cttggttcaa gtagtggcgg tagcgttttt ccatttgatc
     1621 actatctact gttttcccaa attgctggaa aagtaaccca aaacgactgc ctattatttc
     1681 ttctcgtgat agtctgcctt tttcaaactc ctgccataaa tgatgattca aagccttata
     1741 gctggtttcg atttctggtg tcaatgtgat tccttgttct gcaaacaaag cacttaaagc
     1801 attggcttca gttaattgaa aatctaacaa tgtatcatct acatcgaaca ataaagtttt
     1861 atatttcatc tttttgacct cctatttaaa ataaatactt aactttttca tctctgctaa
     1921 tgtcaacgat cgatactcgc ctactcctag ctgttcatct aattccaatg gtcccatgct
     1981 tagccgtttc aatgcggtca ctttctttcc ttgggctaag aacatctttt tgacttgatg
     2041 gaattttcct tcttggatcg tcaataaaac cgtactttgg tgcttgtctg ctttgagtat
     2101 ttttaaatca gccggcttac attgataccc atcagcgaat acgattccat tttcaaatgc
     2161 tgttacgtct gccggtgtca ccctttcgtt cacaactact tgataagtct ttgaaacctt
     2221 tttttctgga agtagcattt catatcccaa ttgtccattc gaagtgatca gcaatagtcc
     2281 tgttgtatct ctatctagtc ttcccactgg atataatggt tggaaacgat cggcacttgc
     2341 aatcagatcg attactgtcg tcttttctgg atctgataca gctgtaacag ctttttcagg
     2401 tttattgaga agataatagg actcatttgt ttctaagtgt tcacctgcta cttcgatttg
     2461 atgcagtaaa ctatctacat ttcgattttg tcgaaattcg actgtgccat ctatcatcac
     2521 ttgcttcaaa gcaaacagcc gcttcatttc cttgcgtgtc gtttctaatt tttcttctat
     2581 taatttatct aaacgcatgc tgtttccttt cttattacta ctaattaatt ataccttgaa
     2641 tattcttgct tgcaaataca tcatcatagt tattccttga cttaattaac agttcacgtt
     2701 acggttaaat agtaaagtga attggatttc tcatacacag cgtaatgacc tggtggagaa
     2761 aagttgaatc ctgactttct aaggggagat gcgcgcatct ctccttttgc gcttttttaa
     2821 gcgccctgcc ttcgatgatg tgcacgagat tagcctttag aattcactta aataaaatct
     2881 gtccgttacc aaagcagaca gtcacatttt aaggaggaat tttggttatg tcagtatcat
     2941 tagaagtaag taaaagagaa gtacgtcccc gttcattaag aaacaaactt cgtcacgaag
     3001 ggaaagttcc agctgttgtt tatgggtata aagtagaaag tacccctatc tcattcgatg
     3061 gtttgacact aagcaaattg ttgcgtgaac atggagcgaa cacagtcatc acgatggaca
     3121 tcgatggaca aaaagtcaat acattggttc acaagatcca aacgaacact tttaccaatc
     3181 atttcgaaca tgcagaattt ctatctgtaa acatgtcgga agaaacagaa gtcgaaacag
     3241 aagtcgttct tgttggcgaa tcagtgggtg ctaaatctgg tggagtgtta gctcaaaact
     3301 tgtatacagt agtcgtatct gctacaccag acaaattgcc tgaacgaatt gaagttgacg
     3361 tgacaaactt ggaattaggt gattctatta cagtagccga tcttccaaaa aacgacgatt
     3421 acaaattcgt gaccgacagt gaagagcaaa tcgctgctgt agtcgctcaa agtgaagaag
     3481 aaagcacagg tgaaggtggc gaaccagaag tgatcggttc aacagaagaa taatactgaa
     3541 acaatcaggt ggtgttagaa gcggcgggtc agcagctttt aacacgcctt ttttatttct
     3601 aatcgtttat tttcatatgg ggcttccact tttgtaaatg gctttacaaa cgaaaaatcc
     3661 taatttacca agcttttcct cgcatacttt tatcgtacct ttataaaaat ttatactttg
     3721 aaaaaaagta ttcaattatt gttgcatttt tcctgattct ttattagaat gaaccctcgt
     3781 tgaagtaagt atttctagtt tatcctagga ttaattataa aaataaaaca ttcgttgatt
     3841 cgaaggaggt taaacagatg tttgaaagtt ttgatagcag ccgcaaccgt tacgcttcag
     3901 ttggtgtcgt atcaagtttg ccagatgaat tgattgacag tatatggttt attattgacc
     3961 tagatcttaa gggagtcatt ccattagaaa atatattaac ctttgatttg atcgaaaatc
     4021 gtggaaaagt gacgatgcat ttttctcaag caggaagcaa tgtggaaatg gatattgatt
     4081 tgccgtttgc ctattcgaac caatttcctg ctcaagtata cgcctatgat gacggtacga
     4141 gagaaacgat ccttttacca agtgagatta gacaatatta aggtgataga taaacgtgga
     4201 tgtcccttat tttacagacc aggccaagtt gttgattctt tgactcaatt cttggcttct
     4261 ttttatggag tttcataaat tatcattgat ttctaattgg tattctcagg ataaacgcta
     4321 tatactacta actaaatttt aaagatataa ggagtttaaa agatgcgcga tttaacaaaa
     4381 ggaagccccg ctaaactgat tttgatgttc actgttcctt tactgatcgg aaatgttttt
     4441 cagcagttct acaatatgat cgatatgatc atcgtgggtc agacgctagg taaaaatgcg
     4501 ctggcagcgg ttggtgcaac tggaagcctg acatttttga tcattggctt tgcccaaggt
     4561 ttaacagctg gtttagccat tattaccgca caacgttacg gtgcaaaaga ttatcggggt
     4621 ctgaagaaaa gttttgctgc tagtgttgtc attagtcttg tcgtcactgt gattttaact
     4681 gtcctaagtc tcctgtttat tcgtccgatg cttcaattga tgcaaactcc tcctgaaata
     4741 cttgatcaag cacaaacatt tatttcaatt attttattag ggatctttgc atcagtcagt
     4801 ttcaatttgc tttctaatgt cattcgcgca cttggtgaca gccgcacacc actattcttt
     4861 ctgatcattg cggtcatcat caacgtcgta cttgatttga tctttattat ctattttggc
     4921 atgggtgtgg aaggtgcagc tattgctaca gtaatcgcac aagtttcttc cagcgtactg
     4981 tgtctagtgt atatcaagaa aaaaatgcct ttgttacagt tacgcaaaaa agatttcaaa
     5041 tttgaaaaag aagagtttgc tgtacaccta aatgctgctt tgccaatggc gtttcaatct
     5101 tcgatcatcg ctgttggtgc tatcgtgtta caagcagcac taaatagtct agggacagat
     5161 gtcgtagctg ctcaagccgc tgctggtcgt atcgaccaat ttgcaaatca gccgatgatg
     5221 tcatttggta ttgcaatggc aactttcact gcacaaaact atggtgcgaa agaatatggc
     5281 cgaatcttgc aaggggtacg tcaaacttta ctgatgagtg tcggatttag tctcttagca
     5341 ggtgctatcg tcattttctt cggtcatttc tttgttcaat tgtttgttag cccatcagaa
     5401 acacgtgtat ttgaattagc acaaacctac ttcaatatca atgggtctct ttactggatc
     5461 ttagcagtcc tgttcatttt acgctacaca cttcaagggc ttggacaaag caagatccct
     5521 acgatcgccg gtatgatgga attgcttatg cgttcttttg cagctatcgt tcttacagga
     5581 atgtggggat acccaggagc agcagcagct tctcctttag cttggatcgg ttctgttgcc
     5641 gtgttgcttt actcttatat gcgttcgatg aaacaattga aacacatggg tgaagaggaa
     5701 catttcacat tagaacatgc agaagcaaga tagccttgat taaaagaaat cgttgcggct
     5761 aaaaatgaaa acttttattg aaaatatctt cttcgtttac cgaaaacagg ttttttagat
     5821 aacaaaaagg ctgtgacaaa aaatgtcaca gccttttttc ttttattcta tttttctttc
     5881 cgtggactct actttgaaat aatcgatatg tgcatgctgg tattctaata ccgtgaaacg
     5941 gaatgatgca atctcaatga ttgttcctgc ttttatttct ttatatcggc tagaagcaaa
     6001 gcccgcaagt gtagtaaaat tcgtttcttc aaactctgga atctctacat gaaaataacg
     6061 ctggaaatca tctaatgtta gtttgccgga gatgatataa gagccatctg actgttccgc
     6121 tatcaatgct tcgtccaaat ctgtctcatc tcggatttcc ccgacgattt cttctaaaat
     6181 atcttcaatc gtcactaagc cgcttgtccc tccgtattca tcttttaaga tagcaatatg
     6241 ctttcccttt tttcgcatga tcgtcaataa tttttttata gggatcgttt cagttgttac
     6301 gatcggctct tcaatcaact gttctactgt cagctggtca tctttataag attgcttgat
     6361 caaatctggc aaggtgacat aacctaaaac attgtctttt gattctaaga taaccggata
     6421 gcgagtatgt ccttgttcaa tcgaagcttt tatcgcttct ttgacggttt ctgaagcttc
     6481 gaatacttgc atggacgtac ggtctacttg tatatctttt gccaaggtat catcaaattc
     6541 aaacacgttt tccaaataat gatattcttc tttggtcaac tctccttttt cataagaatt
     6601 caatgcgact aatgtcagct cttcttgaga caatgtctct tctccttcag atacgagacg
     6661 cacacctaat agcttcccta gccctgcagc tgaatgattc aatacccaaa taaaaggaaa
     6721 cattacttta taaaaataat gaagtggttt aactacgaac aagacgacct tctccgtatt
     6781 gacgatacta tagctcttag gaagcagttc gccaataacc acatcgatat acgtaactaa
     6841 aataaatccc agcgccacag agattgtact gctaatagcc gaactaaaag gtagtttacc
     6901 gatcactggt tctagtaaag ccgcgacaga tccttcaccg acccacccga tgatcaaacc
     6961 ggttagcgta attcctaatt gactagctga taaatagtta tctaaatggt gtgtaacatg
     7021 aagagctagc ttggcgttct ttacattttc cttttctagt tgttccaatc ttgatcgtct
     7081 gattttcact aaagcaaatt cacttgctac aaaaaatgaa gtaattgcaa tcatcaaaaa
     7141 taaaataatg aaattgataa ccatattctc ccgcctttct ctttttctat tgtacccttt
     7201 ttctacaaaa agattgaaaa taatgcgtat tcatatttag gaataaaaaa ggagatgcgg
     7261 catttttcag ccacatctcc ttatcacttc aaaatcattg cgttacttgt tgctaaacag
     7321 ctttttcatg acctctgata aacggtgttc aagtagctga cattcgacat tagttcaggt
     7381 caaagcgcta ctttcacaac cgctctttca accatgtttt ccgagtattt ccaatgataa
     7441 gcggaacagt ttcatgtttt acttcgctgt actctagccc aaaccatgtt tctggcgagg
     7501 tagcgatttt tttgattgcc agttttgcct gcatgatttg acgttgccat tttcctagag
     7561 ttgtcacatt ggaaactact tcatcaatca ggacaaattg gaaatcgcca acttctcgcc
     7621 ctggtgtcag agagtatctt tgtggctgtt ttggcaaacg gccttgtttc atcaaatcat
     7681 ggatgacttg gcgaacatat acgttgattt cctgttggac cctgaaacct aagaacagat
     7741 tcacttggac aataaaatca gtgtccatca tatctacttg atattctttc gtatatggct
     7801 catccgtcac ttccacatga acaaaccaat agactcgtgc tcttttaggc cgtttatcta
     7861 aaatcgaata gatgaactgt cggccaactt tatcttcagc catatcagga accaagaaga
     7921 ccacatttgt ctgatagatt ggtaaagaag tatcatcacg taattctcta agctgcggga
     7981 tataatctct caaagacaca tcctcggttg acgattcgcg aataatgttc ccttgttccc
     8041 aaataaacat cacgactaag atgatcaaac caatcagaac ggctacaaaa ccgcctcgga
     8101 agaatttgac aacacttgag ataaagaaaa cgacttctat cgcaccgaag aaaaaggtaa
     8161 ctaaatatgc aagccaacga gaatgcccat tttggataag ataaaagtaa agcagtgctg
     8221 ttgtcatcaa catcgtcaca gtgatcgata gaccgtaagc agcttccata tgatgagacg
     8281 ttcggaatgt gacaaccact agagaacagg cgatccataa aatcagattg actgctggga
     8341 tatacatttg cccgatactg ttcccaggat acatgatctt taaccgaggc agcagtttca
     8401 acttgattgc ttctgaaact agcgtaaacg aacctgtaat caatgcttgc gaagcaatga
     8461 ctgcagcgac tgttgcaaaa gcaacgccga tcacgatcca actttgaggc atcatttgga
     8521 agaatggatt caggttcttg atttgctgtg cttcagggtt ttgataaaca ttcaacaacc
     8581 acgcagcctg cccaaaataa ttgataacca aacaaatttt gacgtaaggc cagctcagac
     8641 gaatatttcg tttgccgaca tgtccaagat cggaataaag cgcctctgca cctgtcgtag
     8701 ccaggaaaat atttccaaga atgaacaagc ctaatttgtt atctgcacta aaaagcagct
     8761 ggatagcata ataaggattc agtgcacgga taactgtcca gtcttgactg aagttcatta
     8821 atcccattac gcctaaaaaa gtaaaccaag cgaacatgat tggtccgaat gcttttccga
     8881 caatatctgt accaaagcgt tggaccatga ataaaacaaa aataataact aaagtgataa
     8941 tgacgatcac gttctggtta ttaccgaacc ggtcaaaaaa ctgcggaatt cctcgcaagc
     9001 cttcaatcgc agtcgttacc gtcacagcag gggtcaatac cccatctgct agtaaggcag
     9061 ctcctccgat cattgcagga atgatcaaat atttcccacc ttttcggaca agtgtgtaga
     9121 gtgagaaaat tccaccttca ccatggttat ctgcattcaa tgcaatcacg acatatttga
     9181 ttgttgtcag tagagtcaat gtccaaaaaa tcaatgagac tgatccgata acaaaatcag
     9241 gattgattcc tgacaatccg ccattatctt caacaatcga tttcataacg taaagcggac
     9301 ttgtcccgat gtctccgtag acgactccca tcgctacaag caggccagcc attgatatct
     9361 ttttggtatt gtgcgttgtt gccacgtttt tccctcattt cttgttatat ggatatcctt
     9421 taggtaagct tgaatgatag atgatattct tatccatgac acccgctttc ctaaattgct
     9481 tctaattatc caagtaacac actgaagtgt caacttattt gaataaaatt attctttctt
     9541 tggacgttct ctgatacgtc ttgccacaaa tgaataaaga gcaaggcaaa aaattgcacc
     9601 caataatgct ggtatcaaat aaaaaccgcc gagttccggt cccagtggtc ctagtaaaaa
     9661 ttcgccgagc caactaccta aaaaaccgat aatgatattt ccagatacgc ctcccgggac
     9721 ttcctttcct aaaattgcgc cagctatcaa acctaatata ccgccgataa ataacgacca
     9781 taaaaaattc agcatctcct ttccctcctt tatccatatt tctcactttt attttacgct
     9841 ttattcagaa ggactccaaa aatatgcgcc aagaaactcc agaaaaaaag aagttgtaac
     9901 aaaagtgttc agcttatttc cgtagaagct
//
LOCUS       DACEXQ010000085         9897 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.085, whole genome shotgun sequence.
ACCESSION   DACEXQ010000085 DACEXQ010000000
VERSION     DACEXQ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9897
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.085"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(171..521)
                     /locus_tag="IXH04_001501"
     CDS             complement(171..521)
                     /locus_tag="IXH04_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852112.1"
                     /translation="MINNYLHYKAINWNVIEDELDNVVWERATSLFWLDTRVPIENDR
                     SKWANLQLQEQEQLNRLLILLTNIATYQSNELGEIIRDSARSQQEIAIINNFQFTEMV
                     HTKAYNRILSIRQF"
     gene            complement(570..1013)
                     /gene="nrdI"
                     /locus_tag="IXH04_001502"
     CDS             complement(570..1013)
                     /gene="nrdI"
                     /locus_tag="IXH04_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016896697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAQ1852113.1"
                     /translation="MQIIKVYYISLSGNTTSFLADLDTYCQKQYQKKVDYVNVKDLVK
                     LGKPLTFDINESYFVFLPTYLEGGNGIETGDVEILTTPLRQVITYNKNRDYCLGIIGS
                     GNRNFNKQFCLTAYQYADDFGFTVLDEFELKGTTEDIERIAETMQ"
     gene            1160..1825
                     /locus_tag="IXH04_001503"
     CDS             1160..1825
                     /locus_tag="IXH04_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016896696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ1852114.1"
                     /translation="MKNSGCLCTVPLFASLSLDEKNEIKCLLHHQTFKKGEIIFSPSS
                     EEQLLIISLGAMKIYRISRTGKEQLIRIVKEGDYDGENYLFGLPNDCLYGEAVEETKT
                     CTLYKSDFNKVMARYPQLSYRLLQLNACKTVAVEKQLQLLAFDRIEDRLALYLNQLSI
                     AAKMGEPITLPISMRELANYLATSPETISRKLRDFQERQLITRKGKQITLLEEFWFIF
                     NYI"
     gene            1815..2270
                     /locus_tag="IXH04_001504"
     CDS             1815..2270
                     /locus_tag="IXH04_001504"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HAQ1852115.1"
                     /translation="MIYKEEDIKIGIVIARFNDFLTKSLLSGCEDGLTRSGVLTENID
                     VAWVPGAYEIPLTAATMAETGKYDAIITLGAVIRGATSHFDVVVNQASSGVSQVGLTH
                     KLPVIFGILTVENIEQAIERSGTKAGNLGFTYATNTIEMINLFKEIKQQ"
     gene            2420..3799
                     /locus_tag="IXH04_001505"
     CDS             2420..3799
                     /locus_tag="IXH04_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HAQ1852116.1"
                     /translation="MTIQFKQTLAPTKDNSGKPIILSDATFAQRNEKVLQLMREYDFS
                     SLMIYADKEHGSNFEYLTGFIPRFEEAIQVINQDGTSTLILGNENYNKTSMARTQSEG
                     ILCPFFSLPNQPMDFTKTFADYLKDVKVDASKRIGLVDWKLLSNDMDNFHNHHSIPAF
                     IVDGIHEVFGSDKVVNATQLYMHPKEGARVTNNAEEIARYEYGASLASDAVLEAMNQL
                     TVGQSELAAGNHLNRDGQYPSVVTIAAFGDRFEGANIYPTDNTLNDGDKVALTVAYKG
                     GLSSRSGYAVKEESQLAEHDPGYLEEVVYPYFQAYKWWLDNLTIGRKGGDFYQAFSDF
                     YPQATYGWNLCPGHLVADEEWLSSPFYQDSDATIQSGYIFQLDFIPSQAGHNGVSAES
                     TIAVADAALREQIKAEQPELWARICNRKDYLKEALGLELSEHLLPLASTFAYYRPFLL
                     DKKTAIYFG"
     gene            3936..4778
                     /locus_tag="IXH04_001506"
     CDS             3936..4778
                     /locus_tag="IXH04_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ1852117.1"
                     /translation="MPLDEQIELLFTIDEGYLNPLKVALTSIRQNNPGQAFRIWLIHE
                     SIAMHTIRELQKLTDYLQFGFEAIKIDGSRWNSAKTEDRYPKEMYFRLLAGEILPKEM
                     KRVIYLDPDILVVNPLLDLWQTDLEGYMLAAATHVGLTDVSTRVNQMRLDVDHAYYNS
                     GVMVIDLDKAREIIKWSDIAQMIEKYNLLLILPDQDILNRLYGKYTKEIPEEIWNYDT
                     RKYMRYFTKSLAQHDIHWVMAHTAILHFCGGPKPWDDKHDNRFTSLYLTYQNQLKRIE
                     DLMK"
     gene            4852..6300
                     /locus_tag="IXH04_001507"
                     /pseudo
     CDS             4852..6300
                     /locus_tag="IXH04_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            6415..8187
                     /locus_tag="IXH04_001508"
     CDS             6415..8187
                     /locus_tag="IXH04_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ1852118.1"
                     /translation="MNNNETKKTYELRITADEIKSTDPTFTMKNLSLGDNYQPKDAYF
                     RTAEGEITRRNYGKNERKISNQTLPRLAIQMFEQSIVSLPESERATFPVCRYHSGDEI
                     IRGIFPDVDSFKKKRKSIEFLIYKYNEDKQIVIYCWSIFSTLLFVQECLKRFGTSGDE
                     FILVYKGKEKKEIRVGETQTMTNHLLKSYVLHLLKSKNLILRGAPGTGKTFLANEMAA
                     DVVSNGRTTQINELSEDEKSRIGFVQFHPSYDYTDFVEGLRPTTSDDGVVSFTLKDGS
                     FKSFVEKAKKTKKVNGQDNFEEAWAKFFDAVTEASIEGEGYNKLKTLTGKPVRNLLAY
                     ERNEIQGVYPAKTNTYLNHDQIYNVYRGLPGTPKGGFDAYRKAIVKHLKEEFGLKNYT
                     ASTVDSTNNQNYVFIIDEINRGEISKIFGELFFSIDPGYRGHKEGVYTQYANLHENPE
                     EKFYIPNNVYIIGTMNDIDRSVDTFDFAMRRRFTFLEIKAEDSAQNMLTQEKTKELMT
                     RLNEAIVAKEKGGLSTDYQIGASYFLSIDNGEETMEQLWDTKLYPLLKDYFHGEHKAK
                     EKLEILEKVYSNDGDLDEAETSTR"
     gene            8162..9454
                     /locus_tag="IXH04_001509"
     CDS             8162..9454
                     /locus_tag="IXH04_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine 5'-monophosphate oxidoreductase"
                     /protein_id="HAQ1852119.1"
                     /translation="MKLKPLHDNQEINDSVVQHPIITNLVNRDLSSLEKENFIIFPQQ
                     LNQSKDLSEDNLIFQSRNGKIWTCNVVGILSDSTDELRIHSRFANQMKHEDFFLRYML
                     QRVLNYNVINDHFSASKKMTYYDLLVFLFPYYLNEAMRKGTYKEYVKREYNNANIKGA
                     VDVAKHIRSNVPFVGKVAYRTREFSYNNHLTQLIRHTIEKIQNEYDFLLSGDEDTKEN
                     VVLIKQTTPGYARLNQFNILQENILHPVKHSYYEEYSALQQICIQILSEEKSGFGSDK
                     NQIHGIIIDVSWLWEEYIGKVTGWKHYGRDKGLATMHLFQEPNRSPRYPDFTFNNIPI
                     DTKYKKHLDTRNDYNQLVTYIHIMNLDQADTMKGGFLQPTSDPLPANHGYKKIGILSG
                     LGGEIFTYHFFIPQAASSYLEFVEQMREVEAALQTTFQ"
BASE COUNT         3455 a         1600 c         1876 g         2966 t
ORIGIN      
        1 agatgggcta cttttgaaac aaagtgtgat tgattgaaat aagcttctat catcacgagt
       61 tcagttggtg taagatgggt ataggtcatt tgtactcact ccttataatt ctttcgacgg
      121 aactattttg agtgtagcac aaatgacttt tttagttgtc tagcttaatt ttagaattgg
      181 cgtatgctta aaatgcggtt ataggctttt gtatgaacca tttcagtaaa ttgaaaatta
      241 tttatgattg caatctcttg ttgggaacgt gcactatctc gaataatttc tcctaattca
      301 ttggattgat aggtagcgat attagtcaaa agaatcaaca agcgattgag ctgctcttgt
      361 tcttgtaatt gtaaatttgc ccattttgat cgatcatttt cgatggggac acgtgtatcc
      421 aaccaaaaaa gtgaggttgc tctttcccat acaacgttgt ctaattcatc ctcaatgaca
      481 ttccagttaa ttgctttata gtgtaaataa ttatttatca tagtacctcc taaaatattg
      541 tttgaaaggg gatgatatgg tgtcattttt tattgcattg tttctgcaat tctctctata
      601 tcttctgtcg taccttttag ttcaaattca tccaacacgg taaaaccaaa atcatcggcg
      661 tattgatagg ctgttaggca aaattgttta ttgaagtttc tgttgccact accaataatt
      721 cctaaacaat agtctcggtt tttattgtaa gtaatcactt ggcgcaatgg tgttgtcaaa
      781 atttcgacat ctcctgtttc aataccattt ccaccttcaa gataagtagg cagaaataca
      841 aaataggatt cattgatgtc aaaagtgagt ggttttccta atttgactaa atcttttaca
      901 ttcacataat ctaccttctt ttgatattgt ttttgacaat acgtatctaa atctgcgaga
      961 aaactcgtgg tatttccact taaactaata taatagactt taattatttg catgtatcac
     1021 acctcttttt aataaataac cacatatata gtgcttgttt taattagaaa cgctatatat
     1081 agtgtttatt gtacatgaca tatcgttata gaaaattgac gatggtcaat tattttgatt
     1141 ttagaggaga gaaaattaca tgaaaaattc aggttgttta tgcaccgttc cactcttcgc
     1201 ctctctttca ttagatgaaa aaaatgagat aaagtgtttg cttcaccatc agacgtttaa
     1261 aaaaggagaa attatttttt caccaagtag tgaagaacaa ctgttgatta tatctttagg
     1321 ggctatgaaa atatatcgaa ttagtagaac tgggaaggag caattaattc gtatcgtaaa
     1381 agaaggggat tacgacggtg agaactatct ctttggtcta cccaatgact gcctatatgg
     1441 tgaagctgtt gaagaaacca aaacttgcac tctctataaa tccgacttta ataaagtgat
     1501 ggcacgctat ccacagctca gttatcgatt gttacaatta aatgcttgca aaacagtagc
     1561 tgtggaaaaa cagttgcagc ttctagcgtt tgatcgtata gaggaccggt tagccttata
     1621 tcttaaccaa ctatctatag cagcaaaaat gggggagcct attactttac ctatctctat
     1681 gagagagttg gcaaactatc tagctaccag tcctgaaact atttctcgta aattaagaga
     1741 ttttcaagaa agacaattga ttactagaaa aggaaagcag attacactat tagaagaatt
     1801 ttggtttata tttaattata tataaagaag aagatattaa aattgggatt gtcattgcac
     1861 gattcaatga ttttttaact aaaagcttac taagtggttg tgaagatggc cttacgcgtt
     1921 caggcgtttt gacagaaaat atcgatgttg cttgggtgcc gggggcctat gaaatcccac
     1981 ttacagccgc aacgatggct gaaacaggta aatatgatgc cattattacc ctaggtgcgg
     2041 taatcagagg agctacaagt cactttgatg tggtggttaa ccaagcgtca agtggggtat
     2101 ctcaagtagg cttaacccat aaattaccgg tcatctttgg tatcttaacc gttgaaaaca
     2161 tcgaacaagc cattgaacgc tctggtacta aagctggtaa ccttgggttt acttatgcaa
     2221 cgaatactat tgaaatgatt aatttattca aagaaattaa acaacaataa gtcatcattc
     2281 tatatgaaaa tatctgtaac agcatagggc tagctttaat tttcaatcat ggtaagatgg
     2341 aagataggaa aataaagcgc attcaattaa ttatcaagcc tttctgatga tttgcgcaaa
     2401 aagcttaagg agacttcata tgacgattca atttaaacaa acattagcgc caaccaagga
     2461 taattctgga aaaccaatca ttttaagtga cgctaccttc gctcaacgta atgagaaagt
     2521 ccttcagtta atgcgtgaat atgacttctc atcattgatg atttatgcag ataaagaaca
     2581 cggtagtaac tttgaatatt taactggttt tattccacgg tttgaagaag ctatccaagt
     2641 aatcaaccaa gacggaacgt cgacactgat tttaggtaat gagaattaca ataaaaccag
     2701 tatggcacgt acacaaagtg aaggtattct ttgcccattc ttctctttac caaaccaacc
     2761 tatggatttc actaaaactt ttgctgacta tcttaaagac gttaaagtag atgcttcaaa
     2821 acgaattggt ttagtagact ggaagctttt atctaatgat atggacaact tccacaacca
     2881 tcattcaata ccagcattta ttgtagatgg tatacatgaa gttttcggtt ctgacaaggt
     2941 agtgaatgca acccaactat acatgcatcc aaaagaaggt gctcgtgtga caaacaacgc
     3001 agaggaaatc gctcgttatg aatatggtgc ttcccttgct tctgatgctg ttcttgaagc
     3061 aatgaaccag ctaaccgttg gccaaagtga gttagcagca ggtaatcact taaatcgcga
     3121 tggtcaatat ccatctgttg tgaccattgc agcctttggt gatcgctttg agggtgctaa
     3181 tatttatcca actgataata cgctaaatga tggcgataaa gttgcattaa ctgtagccta
     3241 taaaggaggc ttgtctagcc gctctggcta tgcagtaaaa gaggaaagtc agctcgctga
     3301 acatgatcca ggatacttag aagaagtggt ttacccatat ttccaagcct ataaatggtg
     3361 gctagataat ttaaccattg ggagaaaggg tggcgatttc taccaagcat tcagtgactt
     3421 ctatccgcaa gctacttatg gctggaattt gtgcccaggg catttagtag ctgacgaaga
     3481 atggttaagc tcaccatttt atcaagactc tgatgcaaca attcaaagtg gctacatctt
     3541 ccaactagac tttattcctt cacaagcagg ccacaatggt gtttcagctg aatcaacgat
     3601 tgcggttgcg gacgcggcac tacgtgaaca gattaaggct gaacaacctg aattatgggc
     3661 acgtatctgt aaccgaaagg attatctaaa agaagcatta ggtttagaat tatcagaaca
     3721 tttactaccc ttagcgagta cctttgccta ctatagacct ttcttattag acaaaaaaac
     3781 tgctatctac tttggataaa aaaatgcccc tcaagattgt ttgccaaact tgagggtttt
     3841 ttgtctattt ttaagtgaat aatgacggac tatccaaacg gaatcgccat aaagatgtta
     3901 atatatgtat aatattataa agaaattgga ggacaatgcc cttggacgag cagattgaat
     3961 tattatttac catagatgaa ggctatttga atcccttaaa agtggcttta acatcgattc
     4021 gtcagaataa tccaggccaa gctttccgca tttggttgat tcacgaatcc attgcaatgc
     4081 atacgattcg cgaattgcag aagctaacgg attatctaca gtttggtttt gaagcgatta
     4141 aaattgatgg tagtcgatgg aatagcgcta aaactgaaga tagataccca aaagagatgt
     4201 atttccgcct gcttgcgggt gaaatattac ctaaagaaat gaaacgcgtg atctatttag
     4261 acccagatat tttagtggtt aaccctttat tagatttgtg gcaaacagat ttagaaggtt
     4321 atatgctagc agctgctaca catgttggat tgacggacgt ttctacacga gtaaaccaaa
     4381 tgcgacttga cgttgaccat gcttattaca attctggtgt gatggtgatt gatttagata
     4441 aagctagaga aattattaaa tggtcagata ttgcacaaat gattgaaaaa tataacttgc
     4501 tattaatcct acccgaccag gatatcctca accgattata tggtaaatac actaaggaaa
     4561 ttccagaaga aatttggaat tatgatacgc gtaaatatat gcggtatttc accaaaagtc
     4621 tcgctcaaca tgatattcac tgggtgatgg cgcatactgc tattctccat ttctgtggtg
     4681 gcccaaaacc atgggacgat aaacatgata atcgttttac cagtctttat cttacctatc
     4741 aaaatcaatt aaagcgtatt gaagatttga tgaagtaaag tgaaacaagt gtttttcatt
     4801 acctacggtg gtttaatgct tatttattat cgttaaatca tgcttataca ctttacaaaa
     4861 tttgattttc gaaatggccg tccaacgaag tctgcgatct taaccacgag ttccattgac
     4921 atggccaaaa agtattatcg agcgatcaaa gaaatgacga aagaacctga ctggttaaca
     4981 aaagagtttc ctaatcagcc cattcgtgaa ggaagaacga tggaggatcc tgattttcca
     5041 cgaatcgcta ttacctattc attagaagaa aataaccaag attcaagtgc ccaacaagaa
     5101 gaaatgaaaa aaattattga ggaatataat cagtattttg atactgcctg gtcattagca
     5161 gacattgagc gttataacgg agacatcaat aattgtttgg cacgaaaaag agcggaattc
     5221 aaacaatttg ggaaacaaat cgatttagta atcgtagtgg atcgtctatt gacaggtttt
     5281 gacgcaccta cgattcaaac cctatttgtg gatcgtaatc tagagtatgc aggattgatt
     5341 caagcttttt cacgtacgaa ccgtacgtat cctgaaaaga caaaaggatt gattgtgacg
     5401 tttcgtaagc cagcgacaat ggagaagaat gtcgaggatg cgacaaaact gtattcagaa
     5461 gcaaaagaag aatcgggttt gatttatcca agttatcagg aatccaaaaa aagattcaag
     5521 caagcgtatg gtaagttaaa ggaggtttca tccatgaaat ccattgatga acatacgcca
     5581 cttgaaatcc gtatcgagta cgttaaagca tttcaagaat tgaatcgtgc gtatgaatcg
     5641 ttagtgactt atgacgatta caatgatgac atggtgcagt cttcaacttt gaacaatcaa
     5701 attcaacttt tggaagaaca aattggcgtg tacgagaccg tgaaggggtc attaatagag
     5761 gaagaaccag aaaaagaggg agaagatttt tcaacgatcg aattttatag tgaaaatacc
     5821 actaagttgt atgatattga ttctacttat attgatcaat tactaggaac ttatgctgcg
     5881 aatagttcag atgcacgaga tgaaattgaa aaagctttgg ctaaattgaa taaaacagaa
     5941 ggagtcaaag aagtttaccg acaaatttta aatgcatttg atgtggggac tttagatagc
     6001 aatgaagata tttttgtcat taaacgtcgc tattttacac aagcaagtga tgaattgatt
     6061 catgcctttt ccaatgagtg gtttgtttct gagaaagaat tacatgcttc taatctgcaa
     6121 tatatgccag gtgaagatcc aattcctaat atgaaagcaa ttatcaacag taaggattac
     6181 gaaggatata aagccaaaca tccaaaagcg aagccattta aatacccaca agaaatgaag
     6241 cgagcctggc gtaaaatgtt agatgatgag ttgattccac ttgagaatga attgaggtaa
     6301 acgagaaaat tgaagaaatt gtatgataaa catgttaata aatgatgctc gaaataaaat
     6361 ttggttttat ttttacattg atacggtaaa taaaatttga aagtagaaga aaatatgaac
     6421 aacaatgaaa caaaaaaaac atatgaacta agaataactg ctgatgagat taaatcaaca
     6481 gacccaacat ttactatgaa gaatctaagc ttaggggata attatcaacc aaaagatgct
     6541 tacttccgta cggcagaagg cgaaataact agaagaaact acggtaaaaa tgaacggaaa
     6601 atttcaaatc aaacattacc gcgattagct attcaaatgt ttgaacaatc gattgtttct
     6661 ctaccagaaa gcgaaagagc aacatttcct gtgtgtcgat atcactcagg agatgagata
     6721 atacgaggga tttttccaga tgttgacagt ttcaaaaaaa aaaggaagtc cattgaattt
     6781 ttgatttata aatataatga agacaagcag atagtcatct attgttggag tattttttca
     6841 actttattat ttgtacaaga atgtttgaaa cgttttggaa catctgggga tgaatttatt
     6901 cttgtttata aagggaaaga gaaaaaggaa atacgagtag gagagactca gactatgacc
     6961 aatcatttgc tgaaatcata tgttttacat ttactcaaat ctaaaaacct tattctacgt
     7021 ggcgctcctg gaacagggaa aacattcctt gcaaatgaaa tggcagctga tgtcgtttca
     7081 aatggcagaa ctacacaaat caatgaattg agcgaagatg aaaagagccg aattggattt
     7141 gtccagtttc atcctagcta tgattatact gattttgtcg aaggtttacg tccaactaca
     7201 tccgatgatg gcgttgttag tttcaccttg aaagatggaa gctttaagag ctttgttgaa
     7261 aaagcaaaga aaacaaagaa agtaaatgga caagataatt ttgaagaagc ttgggctaaa
     7321 tttttcgatg cggtaacgga agctagtata gagggagaag gctacaataa acttaaaaca
     7381 ttgacaggta aaccagtacg caatcttcta gcctatgaaa gaaatgaaat tcaaggtgtt
     7441 tatcctgcta aaaccaatac ttatctaaac catgaccaaa tttataatgt ctatcgtgga
     7501 ttgccaggta cgccaaaagg tggatttgat gcttatcgaa aagcgattgt aaaacattta
     7561 aaagaagaat ttggcttaaa aaactacacg gcgtcaacgg tagattctac gaataaccaa
     7621 aactatgtgt ttatcattga tgaaattaat cggggcgaaa tttccaaaat ttttggcgaa
     7681 ttatttttct caattgatcc aggatatcgt gggcataaag aaggtgttta cactcagtat
     7741 gcaaacctcc atgaaaatcc agaagaaaaa ttctatattc ctaataatgt ttatattatt
     7801 gggacgatga acgacattga ccgttctgtt gacacttttg attttgctat gcgccggcga
     7861 tttactttct tagaaataaa agcagaagat tcagcacaaa atatgctgac tcaagagaaa
     7921 acaaaagaac ttatgacgcg tttaaatgag gcgatcgttg ccaaagaaaa aggtggattg
     7981 tcaactgatt accaaattgg tgctagttac tttttaagta tcgataatgg tgaggagaca
     8041 atggaacaat tatgggatac taagttatat ccattattga aagactattt tcatggtgaa
     8101 cataaggcga aagaaaaatt agaaatacta gaaaaggtat actctaatga cggtgattta
     8161 gatgaagctg aaacctctac acgataatca agaaatcaat gactcagttg tgcaacatcc
     8221 aatcattacg aacttagtta atcgtgactt aagtagttta gagaaagaaa attttattat
     8281 ctttccgcaa cagttaaatc aatccaagga tctaagtgaa gataacttga tatttcaatc
     8341 aagaaatgga aaaatttgga cttgtaatgt cgtaggtatc cttagcgatt caacagatga
     8401 attaagaatt cattctagat tcgctaatca aatgaagcat gaagatttct ttttacgtta
     8461 tatgcttcag cgtgttttga attacaacgt gattaatgat cattttagtg catctaaaaa
     8521 gatgacatac tacgatttgt tggtatttct ctttccttat tatctgaatg aagcaatgcg
     8581 taaagggaca tacaaagaat atgtcaaacg agaatacaat aatgccaata tcaagggagc
     8641 tgttgatgtt gcaaaacaca tccgttcgaa tgttcctttc gttggaaaag ttgcttatcg
     8701 aacacgagag tttagctata ataatcacct aacccagctt attcgtcata ctattgaaaa
     8761 aatccaaaat gagtacgatt tcttacttag tggagatgag gatacgaaag aaaacgtggt
     8821 tctaatcaaa caaacaacac ctggttatgc tagattaaat caatttaata tccttcaaga
     8881 gaatatcctt catccagtca aacatagtta ttacgaggag tattcggctc ttcaacaaat
     8941 ctgtatccaa attttatcgg aagaaaaatc aggatttgga agtgataaaa atcaaataca
     9001 tggaatcatt atcgatgtgt cttggctatg ggaagaatac attggaaaag tcactggttg
     9061 gaaacattat ggcagagata aagggttagc aacaatgcat ttatttcaag agcccaaccg
     9121 gtccccacga tatccagatt ttacatttaa taatatacca attgatacaa aatacaaaaa
     9181 acatcttgat actagaaatg actataatca gttagtcaca tatattcata taatgaattt
     9241 agaccaagca gatacaatga aaggcggctt tctgcaacct acttccgatc cccttcctgc
     9301 aaatcatggg tataaaaaaa taggcatatt aagcgggcta ggaggagaaa tattcacata
     9361 tcattttttc attccgcaag ctgcgtcaag ttatttggaa tttgtagaac agatgagaga
     9421 ggtagaagca gcactacaaa ctacttttca ataagtagga attatagaaa ttttactcaa
     9481 aaaatcttca ttaaaaaatc tgactaaata agttgagaaa acagaatata cttcaatatc
     9541 cagtcattta taaatatcat tcactcatga tacccagctc cgtatcacaa ataatgatat
     9601 tttttaagag aaaaaattat ccctatttta aataatagca ataggtagtg gtagagagaa
     9661 atttataaat aactttgtta tttgctttgg tagccaagta agttgcaaaa gaaaaataga
     9721 tactgttaaa aagataatgt agacatagaa acaaatacgt aaaattttag ttagaatgat
     9781 ggcaaggaga aaaaacatga tagatgaaaa aaagtgttta aaaaatctat tggaagatat
     9841 ggatgatatt gaagaaaaac tatctaaatg gactgggaaa acaaatatat ttcagaa
//
LOCUS       DACEXQ010000086         9846 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.086, whole genome shotgun sequence.
ACCESSION   DACEXQ010000086 DACEXQ010000000
VERSION     DACEXQ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9846
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.086"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..1124
                     /gene="infB"
                     /locus_tag="IXH04_001510"
     CDS             <1..1124
                     /gene="infB"
                     /locus_tag="IXH04_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAQ1852120.1"
                     /translation="EYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKA
                     DPTQRAIGTVIEARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRD
                     KEAGPATPVEITGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLD
                     NLFESLKEGELKEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDV
                     TLAAASNAIIIGFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFE
                     EKITGQMIVRETFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFK
                     DDVKEVKMGYECGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            1151..1498
                     /gene="rbfA"
                     /locus_tag="IXH04_001511"
     CDS             1151..1498
                     /gene="rbfA"
                     /locus_tag="IXH04_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAQ1852121.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(1606..1827)
                     /locus_tag="IXH04_001512"
     CDS             complement(1606..1827)
                     /locus_tag="IXH04_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1852122.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
     gene            2275..4637
                     /locus_tag="IXH04_001513"
                     /pseudo
     CDS             2275..4637
                     /locus_tag="IXH04_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            5005..5928
                     /gene="truB"
                     /locus_tag="IXH04_001514"
     CDS             5005..5928
                     /gene="truB"
                     /locus_tag="IXH04_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAQ1852123.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            5932..6873
                     /gene="ribF"
                     /locus_tag="IXH04_001515"
     CDS             5932..6873
                     /gene="ribF"
                     /locus_tag="IXH04_001515"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAQ1852124.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            7225..8010
                     /locus_tag="IXH04_001516"
     CDS             7225..8010
                     /locus_tag="IXH04_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1852125.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            8023..8817
                     /locus_tag="IXH04_001517"
     CDS             8023..8817
                     /locus_tag="IXH04_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAQ1852126.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKN"
     gene            complement(8997..9449)
                     /locus_tag="IXH04_001518"
     CDS             complement(8997..9449)
                     /locus_tag="IXH04_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1852127.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
BASE COUNT         3328 a         1685 c         2109 g         2724 t
ORIGIN      
        1 gtgagtatga attaattcct gaagcatggg gtggagaaac gatttttgtt gagatctcag
       61 cgaaattcgg ccaaaacatc gaagaattgc tggaaatgat cctgttagtc gcagaagtag
      121 aagatttaaa agcagatcca acacaacgcg caatcggaac agtgatcgaa gcacgtcttg
      181 ataaaggtaa aggtcctgtg tcaaccttac ttgtccaaga aggaacactt cacgttggtg
      241 acccaatcgt tgtcggtaat acttacggac gtgtccgtgt aatgacaaac gatcttggcc
      301 gtcgcgataa agaagcagga cctgcaacgc cagtagaaat cactggcttg aatgatgtac
      361 ctcaagcagg tgaccgtttc gtagtcttcg aagacgaaaa gacagcacgt gcagcgggtg
      421 aagaacgcgc aaaacgtgca ttattggaaa accgtgctgc cacaagccgt gtgactttgg
      481 acaacttatt tgaaagcctg aaagaaggcg aattgaaaga agttaacgta atcatcaaag
      541 cagatgtaca aggttcagca gaagcactgg ctgcatcatt gaagaagatt gatgtggaag
      601 gtgtccgtgt aaaaatcgtc cattcagctg tcggagcaat caatgaaagt gatgtaacct
      661 tagcagcagc cagcaacgct attatcatcg gtttcaacgt tcgtcctaca ccgcaggcaa
      721 aagtgcaagc tgatactgaa gaagtcgata ttcgtcttca ccgtatcatc tataaagcca
      781 tcgaagaaat cgaaactgcg atgaaaggga tgctagaccc tgaatttgaa gaaaaaatca
      841 ccggtcaaat gatcgttcgt gaaaccttca aagtttctaa agttggtacg attgcaggtg
      901 cctatgtaac agaaggatat attcgtagag atagcggtgt ccgtgtaatc cgtgacggaa
      961 tcgtaatcta tgaaggccaa cttgcaagtc tgaaacgatt caaagatgac gtgaaagaag
     1021 tcaaaatggg ttatgaatgt ggtgcgatga ttgaaaaatt caatgacatc aaagtagatg
     1081 acgtaattga aggatttatc atggaagaag tcaaaaatga ctaaaaactg ataaaaggag
     1141 gccaatcatt atggctaact accgtgatcg ccgagtcggt caagaaatct taaaagaagt
     1201 caatgatatt ttgcgcaaaa aggtccgtga ccctcgtgtg gagaacgtaa cgatcacaga
     1261 tgtgaaagtt acgggggact tacagcaagc aacgatctat tacagcattt tatctgactt
     1321 agcatcagat aagaaaaaag cacaagatgg attgaataaa gcatcaggat tgattcgtcg
     1381 agagttaggg cacaatctaa gtatttataa gacacctgaa ttgttctttg aacttgatga
     1441 atcagttgtc tatgggaacc atatcgatga attgcttcgc gacttgaaca aagactaatc
     1501 aaaaaatccc gcaaatcctg tgaaatcagg ggttgcggga tttttttgtt ttcattaaga
     1561 cgtattacct aagagttcca tgactttagt ctggtagcgt gtcagctaac caaaaaagag
     1621 aaaaagaaaa gtaaaaaatt cgactttctt ttttctatag tatataaatt tttgcatata
     1681 gataaagatc aagataccga taacggaagc tagattgtaa tctattttag tgacagtcag
     1741 gtatagccaa gtaactagaa gatcagtgga acctgcacca taaaagtttg agtttgtcgg
     1801 tccgttgaca gttaaaagaa aaatcacgtt gaagttattg atatttccga ttacgccgct
     1861 tcgatttggt cacgatccaa attttggata atccctgtag aaattagcat agtagctggg
     1921 ataccaattc acatattgac aaagataaca gagactttta cataaaacgg atcaatcagg
     1981 aaaggaattg ctcgatccag ccccagtttt ccaacaatgt gttaactggc ctagcgtcat
     2041 tcagcaaatt atgcatgatg aggagcgaaa tgaactgcgg aacagccatt gtgataacga
     2101 caagtttttt ggatcaatgt aaaaaacgtc agcgaaagga aaaatttcgt ggttttttat
     2161 acctgttcat ttggctagat ataaaatcca aacaaatgaa cagaagtgct tattaggttt
     2221 caattgggag tatctgcgct agaataagag taataatgaa aaggagtgtt atgtatggat
     2281 tattctagta aagaatactt tgataaaatg acggcttggt ggcgtgctgc taattatctt
     2341 tctgttgggc aactttatct taaagataac cccttactga gacgtacact gaaacccgaa
     2401 gatgtcaaaa aacatccaat cggtcattgg ggaacgattc ctggccaaaa ttttatttat
     2461 gtccatttga accgagtcat caataaatat gatttgaaca tgttttatat tgaagggcca
     2521 ggacacggcg gacaagtcat ggtatcgaat gcttatttag atggaagtta cacagagatc
     2581 tatccagaag taacggaaga tgagacagga atgcaaaaat tattcaaacg attttcattt
     2641 cctggaggca ttgcttctca tgcagcacca gaaacaccag gttctatcca tgaaggcgga
     2701 gagcttggat actctctttc acatgcagtc ggagctgtat tagacaatcc tgaagtaatc
     2761 tctgctgtcg tgattggtga tggtgaagca gaaaccggtc ctttagctgg aagctggttc
     2821 tcaaatgtct ttatcaatcc agtgatagat ggagctgttt taccgatcct tcatttaaat
     2881 ggtgcaaaaa tagccaaccc aacgatcttg gcacgtaaat cagatggaga attagcaaat
     2941 tactttaacg gacttggctg ggaacctttc tttattgaag gaaatgattc agaaaaatta
     3001 aatcctgtta tggctgaaaa aatggatcaa gcaatcgaga aaattaaatc catccaaaaa
     3061 gaagctcgcc tgaagacagc aacagatgtc gtgatgccaa aatggccagt attgatcgta
     3121 cgcacaccaa aaggatggac cggtccaaaa gaatgggacg gggaaccaat tgaaggaacc
     3181 ttccgagcac accaagtacc gattcctgta gatcaagaac atatggatca tgcagatgct
     3241 ttactccgtt ggctgaaatc ctatgaacca gaaaaattat ttgatgcaca aggacgtatt
     3301 ttagaagaaa tcagagaaat cgcgcctact ggtgaccaaa gaatggcaaa aaatcctatc
     3361 accaatggtg gaatcgatcc aagaccgctt attatgccag attggaaaaa atatactttg
     3421 caatttgaaa aaccaggctc cgttaaagca gaagatatga ccgaacttgg taaattcgtt
     3481 cgcgagatca ttgagaaaaa tccagaaaat ttccgtatat ttggaccaga tgaaacaaaa
     3541 tcaaaccgat tgaatcaagt tttcaaaacg acaaatcgcc aatggatgga aaaaatcgaa
     3601 ccagaaaatg atgaatggct ttcgccatct ggtcgcgtca tcgacagtca attatcggaa
     3661 caccaagatg aagggttctt agaagggtac gttctgactg gacggcatgg ttttttgcaa
     3721 gttacgaatc attcttgcga gtggttgact ctatgttgac tcagcacttc aaatggatgc
     3781 ggaaatcaca tgacctttct tggcgaaatg attatccttc gctaaatctc attgcaagct
     3841 caacagtctt ccagcaagat cacaatggat atagccacca agatccagga atattgacac
     3901 atttggcaga aaagaaggcg gagttcatcc gtgaatatct gcctgcggat gcaaatacat
     3961 tgctcgcagt catggataaa gcattccgct cgtcagaaaa aatcaatttg atcatctctt
     4021 ccaaacatcc gcgtgctcaa ttttactcag cagaagaagc agccgtctta gtgaatgaag
     4081 gcttgaaaat cattgattgg gcttcgacag caaaagagga ggaacctgaa ttggtcattg
     4141 ctgctgcagg aacggaatct aacttagaag cattagcagc tgtaactttg ctgctggaag
     4201 aattcccaaa actaaaaatt cggttcatca atgtcgtaga tttattgaaa ttacgtcatc
     4261 caagtcaaga tccgcgtggg ttgagcgatg aagaatttga tcaatatttt acaaaagaca
     4321 aaccgatctt atttgctttc cacggttatg aaacattggt ccgcacgatt ttctttgatc
     4381 gtcataacca tcatttaatg attcatggct acaaagaaaa tggagatatc acgacaccat
     4441 ttgacatgcg tgtagtaaat gaacttgatc gttatcattt ggcaaaagac gcagctttaa
     4501 aaatcaaagg aagtcaagca gaagattttg cgaaaaaaat ggatcagaaa ttacaagaac
     4561 atcaaaacta tattcgtgaa aatggtattg atctgccaga agtattggat tggaaatgga
     4621 aaaatcttga ccaataaaat gataaaatcg atcccgcaaa atagaatttt ttacattgat
     4681 taagaaactg aaaaaataaa taaaaaaaac ggaaaaaaga ctggaagaat ggccagtctt
     4741 ttttttcgtt tttgggaagt gaaaaccaaa ctatttacct gtaagccacc cataaatcca
     4801 atcaaatggt gcaaagaaaa aatcttccat tctattccct cctttaaaaa agcataacat
     4861 gatttctatt tacgtgagca tgaattttgt tacgttttta ttgccgaaaa ataaaaaccg
     4921 ttcattatga tgtcaaattc gtcagtaatt gatgcaatgt gttataataa ttcagttaaa
     4981 tcaaaaatga aatggagaaa agacatggac gggttattac ctttatggaa agaacgcgga
     5041 atgacaagtc atgattgcgt attcaaatta agaaaaatct tacatacgaa aaaaatcgga
     5101 catgggggta cgcttgaccc agacgtagac ggtgtgttgc ccatttgtat tggaaaagcc
     5161 acaaaagtta tcgaatattt gacagattct gggaaaatct atgtaggcga gataactcta
     5221 ggtttcagta caacgacgga agatcgatcc ggagaacggg tagaaacaaa accagtagat
     5281 atgtctttat ctgaaaaaaa aatagatgaa gtgatggctt catttattgg tgagatcacc
     5341 cagataccac caatgtactc agcggtaaaa gtcaacggta agcggttata tgaatatgcc
     5401 cgcaataatg aaacagtgga gcgtcctgtt agaaaggctc agatttaccg ttttatccgg
     5461 acaagtgaat tgacaagaga cgaagtgaca ggaaccattt cttggcgctt cgaagtagaa
     5521 tgcgggaaag gaacctatgt ccgcacatta gctgtagata caggcaagaa acttggttac
     5581 cctgcgcaca tgtctgatct gacccgaaca gcaagtgccg gtatgtccca gaaggatgct
     5641 ctgacattag aagaagtagc tcaagctgtg aaaaatggga caatcgatga ttgtttgctg
     5701 ccaatagaaa aaggagtgga acggttcaaa cgagtagata ttactgatga agtatggcag
     5761 aaagtaaaaa acggtatgag acttgattat actgtatttg cgctcccaga aatgccgtca
     5821 gaagaaattg ctttgtttta taaaggaaaa gttgtcagta tttatcagcc aaacccgaaa
     5881 gaaaaaaaca aattaaaacc tagtaaagta ttgagaaatg aggaataacg aatgaatatc
     5941 atcaagatca gacatcctta ccaatcagaa caaattccag aagacgatgt cgttttagtg
     6001 ctgggatttt tcgacggcgt gcatatcgga caccagaaag tcataaagac aggtatggaa
     6061 attgcacaaa aagaaggatt gaaattagca ttgatgactt tcaatcagca tccatctatt
     6121 gtctttaaaa agatcgatcc tagcagcgtg aaatatttga ctagccttaa gcaaaaagaa
     6181 gaaaaaatgg ccgctttggg tatagattat ttatatgaag ttgattttac ttcttctttt
     6241 gctcatttag caccgcagga atttgttgat caatacatcg tagggttaca tgcaaaatat
     6301 gctgtttccg gttttgatta tacgtatgga ccaaaagaca ttgcagatgt cgctcatttt
     6361 cctgaatatg ccaaaggaag attcaaaatc gtcacagttt ctaaagaaga agaacaaggt
     6421 gaaaaaatca gctctacccg tattcgtgga ttactagaca gcggacaagt aaaagaagca
     6481 gcaaaactat taggttgcac atatgaggta gaaggggtag ttgtccatgg cgatgcacga
     6541 ggcagactac taggattccc tacagcaaat gtcaaagtaa agagcacggt tcatttgcct
     6601 aaagaaggtg tttatgtgtc ggaaataaaa attggggaaa catggtatcc agccatgggt
     6661 tcaattggac ataatgatac attcggtgaa ggacgggagc tgactgtaga gttgtatatt
     6721 ttggacttta atcaagatat ctatggtgaa catgtcgcgg tacgctggaa tcaattttta
     6781 agagatcaag tgaaattttc tagtgtagag gaattgattg aacaactcaa ggaggatgaa
     6841 caagcaacag ctgaatattt ttctcagcaa taaaacatta tgcataaaat ctcttgttta
     6901 tgtataacta gcaattacaa taaaatgggt gataaataag cccaggaaag gggctgtgac
     6961 aaaagtcttg tcacagcctt tttttccaaa taaatggttt ttcagaagta gcttcttcgg
     7021 aaataagccg aaatttcttg aagctgacca cttctgtcac aacctctttc tttatagaaa
     7081 taaaaggaca ctagcaattc atgaaaacga ttgctagagc gtatcatttg ctttagacta
     7141 tatatcttgg tgtacactca ctttaaggcg gaaataaaag ttatccgtaa taacaggttt
     7201 aggtatgaaa aaggagtggg tactatgtac aaggaattag aaaacaaagt cgcagtagta
     7261 actggtggct caaaggggat tggaacagcg attgctcaac gattcgggaa agaaaaaatg
     7321 aatgtagttg tcaactataa ctcggataaa gaaggagcag aaaaagcagt caaagcaatc
     7381 gaagaggcag gcggaaaagc agtctctgta caagcagatg tcagttctga agaaggaata
     7441 cagcgtttac ttgatacggc tttagaaaat tttgggacac tggacgtttg ggttaataat
     7501 gctgggatgg aaaatcagca tccaacccat gaacttagcc ttgaagattg ggaaagagtg
     7561 ttaaaagtca atttgactgg cgtttttatc ggaacgaaag cggctttaaa ttactttaca
     7621 gagaataata aaaaaggaaa tatcatcaat atgtcttctg ttcatgaaca gatcccatgg
     7681 ccaacgtttg ctcattacgc tgcttcaaaa ggcggggtaa aattatttac cgaaacggta
     7741 gcaatggagt atgcaccgaa gggaatcaga atcaatagta taggccctgg tgctatccgt
     7801 acacctatta atgcggcaaa atttgatgat cctgaacaaa aagaactctt ggaaagtatg
     7861 atcccactag gacgcgtagg tgaaccaaaa gaaatagcgg cgacagctgc atggttggct
     7921 tcagacgaat ccagttatgt tacaggtatt acactatttg cagatggtgg gatgacttta
     7981 tatccttctt tccaaggtgg aaaaggataa gggtgaataa aaatgggttt attgattgca
     8041 ttgatcccgg cagttggctg ggggatccag ccactgattt tgaaaaagat tggtggcaga
     8101 ccaacaaatg agatattagg aactggtatt ggagcggtgc ttgtcggctt actcgttcaa
     8161 ttattcgctt ctccagctgg tatttcagtg actacatttt tgattagttt actttctggt
     8221 gcattttggg ttattggaca aacaggacag tatacatctt tcaacttgat tggtgtttct
     8281 aaaacgatgc caatttcgac tgcattacag ttagtcggaa cctcgttgat tggtgtcttc
     8341 gcttttggag aatggtctgg aagtatgggg aaaatcattg gagcagcagc aatcattctt
     8401 cttgtatttg gttctgcttt aacggcaatc agtgatgatc ctggaaaaaa aggcggtatc
     8461 acaaaaggaa ttagtctatt agcatcaaca agtattggtt attgggtata cagtgcattg
     8521 cctaaactag ttgatgcaag cggactttcc attttctttc cgcaaatgat cggtgtcttt
     8581 ttaggggcag tgatttatgt gattttccgt aaaccaaccg cattttctga agacaagagt
     8641 tggaaagcaa cgatcgttgg agccatcttt agtgtgtcag cattggctta tattttttca
     8701 gcaaatgaaa atggggttgc tactgcatat atcattacac agctaaacgt agttatctct
     8761 acattaggcg gtatgtacat cctgcatgaa aataagacac caaaagaaaa aaattaacga
     8821 ttatcggatt attgctgatc gtagtaggaa gcatggtcac ggtctttatt tgaggataaa
     8881 gatggtaaaa tttcatttat taaaaaaata tttgtttgaa ccaaacagta agtacttata
     8941 aaaaaatagc gctagaattt ccaacatctc tggaatttct agcgcttttt aattgtttaa
     9001 gatacaaggg aaatatctgt ctggatcaat aatttttcat tgatccattg actaaagttt
     9061 acgacataat tgaagttacc accgattagt atctccgcat gattgtcttt ataaagatcg
     9121 tattgtaaaa gacggtcttt tttatttgcg taaacatcgt tatggtactg gactaattgt
     9181 tctcggaatt gattgattgc tgtaaaatca cgatattgaa ggttgaaacg catcgtgatt
     9241 ggtgcattta gcatgaatcg ctgcatttct tttgctgctt cctctgcttg atctccgtat
     9301 aggaactcaa aaagattacg ttcagaaaat ttccattgcc cgccaacctt tgttcctttt
     9361 agttttcctt catgaagata atttctgata gaacgtgtag taacacctaa ttgttcggct
     9421 aattcattta ctgagtaaaa atctttcaat gttcaccctc ctatgtgaat ggttctactg
     9481 acttagttga tatttgctta atcacgaaat gtagaaagaa cgattttaat taaggtcatt
     9541 gtaaaaatca agtcagaaaa ttctaacttt tatttctcta aagaaattcg ttctctccta
     9601 aagtgatatt atatttaaat atacctgaaa ataatgggca gtattatgaa agatcactga
     9661 tattttctcg tattttttcc ttattcaaaa gttttgcagg aaaattgctg aaaaaataat
     9721 tttactaagt agaaaattca aagccattca atcagtgatt tttttcagca caagaaagac
     9781 tccttataaa aatagcagat actttgtcaa ttaagtgttt aaccagggat gataaaaagg
     9841 atagga
//
LOCUS       DACEXQ010000087         9816 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.087, whole genome shotgun sequence.
ACCESSION   DACEXQ010000087 DACEXQ010000000
VERSION     DACEXQ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.087"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(41..361)
                     /locus_tag="IXH04_001519"
     CDS             complement(41..361)
                     /locus_tag="IXH04_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAQ1852128.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(509..2743)
                     /locus_tag="IXH04_001520"
     CDS             complement(509..2743)
                     /locus_tag="IXH04_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ1852129.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            3222..3488
                     /locus_tag="IXH04_001521"
     CDS             3222..3488
                     /locus_tag="IXH04_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAQ1852130.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            3488..5215
                     /gene="ptsP"
                     /locus_tag="IXH04_001522"
     CDS             3488..5215
                     /gene="ptsP"
                     /locus_tag="IXH04_001522"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAQ1852131.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            5346..6425
                     /locus_tag="IXH04_001523"
     CDS             5346..6425
                     /locus_tag="IXH04_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ1852132.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            6442..7665
                     /locus_tag="IXH04_001524"
     CDS             6442..7665
                     /locus_tag="IXH04_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ1852133.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            7754..8641
                     /locus_tag="IXH04_001525"
     CDS             7754..8641
                     /locus_tag="IXH04_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAQ1852134.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            8727..8957
                     /locus_tag="IXH04_001526"
     CDS             8727..8957
                     /locus_tag="IXH04_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAQ1852135.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(9014..>9816)
                     /locus_tag="IXH04_001527"
     CDS             complement(9014..>9816)
                     /locus_tag="IXH04_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotide excision repair endonuclease"
                     /protein_id="HAQ1852136.1"
                     /translation="IHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYN
                     YVHITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLP
                     DLQEMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYF
                     IRRIQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKK
                     EAHLSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
BASE COUNT         3130 a         1739 c         1995 g         2952 t
ORIGIN      
        1 ttttgtccca cgctctttaa ttattatgtt caaaatttgt ttacacggct aatgtagcgc
       61 ctaattcatg ttttggaatc gagatttcaa caaggcctgc caattcgatt actttgattt
      121 catcctcgtg gtaaacggcg tctttgattt tatctaagaa ataattttta gcatcttcga
      181 tttctttcgg ctggatttta cgtttgtcat tgatcagcag aatttcatta tgttcagggg
      241 cttcagtgta gacaatagtg gtatttccgg ctgtgtagac cttgactaga tgggcatcag
      301 tgttttcaag ctggtttaat acaagacggg aatggctgtt tgttacattt accaatttca
      361 tggcgctcac ctcttttatg tatttttaat tgcttgtttc tcttattata atttcttttt
      421 tataaaatga acagtatttt gacttttctg aaaaaagaat gagacggcat ttctcaaaga
      481 aataccgtct cacgctataa aactggattt attcagccgt ttcttcggct gattctttag
      541 ccaaacgttc tggtttagaa gtaacgatga tttttccatc tttatccagt tttgctttca
      601 actcatggat agaagggtga tcaagataaa attcagcgat accatcttcg atttgttctt
      661 ggatcgtgcg tcgaagagga cgagcaccca tagatggatc atagcctaag tcaactaatt
      721 tttctttgac attcgtagga acttcgatgt gaagttgctg tgctgccagc atttggttga
      781 catcgtctag cattagtgtg acgattgtca tcaagttttc ttttgataat gcactgaatt
      841 cgatgatccc gtcaaaacgg ttcagaaatt ctggtgtaaa gtagttgttt aattggttca
      901 atacagaacg agtaacacct tcgcgtgccg caccaaaacc aacgtttgct tctactttac
      961 cagttccggc attacttgtc ataatgatga tcgtatcttt aaagctgacc gttcttcctt
     1021 gtgcatctgt caatcgacca tcatctaaga tttgcaagaa catatgcaaa acatctggat
     1081 gagctttttc tacttcatct aacaagacaa gactgtaagg atttctccgt actttttctg
     1141 ttaattgacc agcttcttcg tatccgacat aacctggagg agaaccaatc aatttagaga
     1201 cactatgttt ttccatatat tcagacatat cgaaacgaat cattgaatct tgtgaaccga
     1261 atagttcgta ggcaagttgt ttcgcaagct ctgttttacc tacaccagtt ggtccaacaa
     1321 ataagaagga gccgattgga cgattctgtt tgtttaatcc aacacggttt cggcgaatcg
     1381 ctttcgctac tcgatctaca gcgttatctt gtccgatgac atgagctttc aagtcatctg
     1441 ctaagttttt cagttgtgtc tgttcttttt cttttaattc acccactgga atacctgtcc
     1501 gttgttcaac gattttttcc atatcttttt cggtaatcac aggtgtttcc tcttcagtga
     1561 gctgacgttc tttcattttt tgaagtttat tgatctgatc gcgataataa gctgcttttt
     1621 cgaaatcttc ttctcgagaa gctaaaactt tttgttcttc tgcttcttgc agttttttct
     1681 cgattgtttt aggatcaacg atttggatcg tcaagttttt cttagaacct gtttcatcta
     1741 gcaaatcgat ggctttatcc ggcaagaagc gatcttggac gtaacgattg gataaagttg
     1801 cggctgcttc gatggcctca tcagtatatt ttacatgatg atagtcttca taacgttttt
     1861 gcaaaccttt caaaatactg atcgtttctt cgacagttgg ttcatctacc cgtacaggtt
     1921 gcatacgacg ttctaaagca gcgtcttttt cgatgatgcg gtactcattt aaggtagtag
     1981 caccaaccat ttgcaattct cctcgagcta aagctggttt taaaatatta ccagcatcca
     2041 tatttccatc accagcagaa ccagcaccga cgatttcgtg aacttcatct atgaatagga
     2101 tcacattttc tgcttgacgg atctcttcaa tcaatttttg catacgttct tcaaattgtc
     2161 ctcgtatacc agtaccttgg actaaagata cgacatctaa acgaatgact tctttgtcca
     2221 ttaatttttg cggtacatca ccatctacga ttttttgtgc taaaccttca acaactgctg
     2281 ttttaccaac acctggttct ccaatcaaga caggattgtt cttggttcgg cgatttaaaa
     2341 tttcaatgac tcgtttgatt tcgtcatcgc gtccgatcac aggatcgata tcaccttgtc
     2401 gtgcttgttc tgtgatattg attccgtatt ctcccaataa accttctgta ttgccttgag
     2461 gtggctgacc accattgaag ttgttgtttc cgccaaattg tgttggtggt gtttgctcat
     2521 aatttccttg ctgttgcatt tggcgagaca tggaacggaa aagatcgtcc aaactgccaa
     2581 atccaaatgg atcattttgg gccatattag tcagacctcc attttgttga tttttgattt
     2641 tttgataaca gctctgacag taatcgagtt gtgtccgctg accattgaca gtggcataca
     2701 agtgaatcgt tgcctcattc ttgccgcagt tttgacaaag catgaatcat tcacgtcctt
     2761 tcacaaaaat gggtacatcc atattttaca tctcgttttt catgaggtaa aagaataacc
     2821 cctagttttt taagagattt atgaggtaga aaaggtgaaa tctctattga ccattcctga
     2881 cctttgactc ttattatact aaaaataaaa agggaagcaa gtaattcgtt tcatatgaaa
     2941 acaaagaggg tcaaaggaaa gaatgataaa cgtcaggaga agagaaagcg acaagaaaaa
     3001 ggaaataaac acgtttgtta taaaatgtga agaaaaggat gaaaaagaat ccggaataat
     3061 ttgaatactt ttatctgaaa ttgaatgaaa actgttgtat ctaacgaaaa aaaatgttaa
     3121 tctttacaga tgaaagggtg gcatttgtta ggattctctg aattactaaa aaagaaaacc
     3181 ctcacataaa aacaacactc acttaaagga gaccgattaa tatggaaaag aaagaatttc
     3241 acgtagtagc agaaactggg atccacgctc gtccagctac actattagta caaacagcaa
     3301 gcaaatttaa ctctgatgta aacttagagt ataaaggtaa atcagtaaac ttgaaatcaa
     3361 tcatgggcgt tatgtcttta ggtgtaggtc aaggttctga tgttactatt actgcagaag
     3421 gtgctgacga agctgatgca atggcagcaa tcgttgaaac aatgaagaaa gaaggcttat
     3481 ctgaataatg gttgaaatgc taaaagggat tgccgctagt gacggtgtag ccgttgcaaa
     3541 agcttacctg ctagttcaac cggatttaac attcagtaag gcaacagtag aagatactgc
     3601 ggctgaagaa gctcgtttgg atgctgcatt agctaaatct actgaagaat tgcagcaaat
     3661 tcgtgaaaaa gcagcgcaaa gcttaggtga agcagaagcg caagtatttg atgcccattt
     3721 gatggttctt tcagatccag aaatggtagg tcaaatcaag caaaatatta aagacaacag
     3781 cgtaaatgct gaatcagcgc taaaagaagt aacggatatg tatatcggta tgttcgaagc
     3841 aatggaagat aatgcttata tgcaagagcg tgctgcagat attcgtgacg ttgctaaacg
     3901 tattttagcg cacttattag gcgttacttt accaaatcca tcaatgatca acgaagaagt
     3961 ggtcgttgtt gcacatgact tgactccaag tgatacagca caattagatc gcaattttgt
     4021 aaaagcgttt gtaacagaca ttggtggacg tacttcacat tctgctatca tggcacgttc
     4081 tttagaaatt ccagcaatcg ttgggacaaa agaaattact gctaaagtca aagaaggcga
     4141 tattttggct gtcaacggga tcgaaggcga tgtaatcatc gatcctacag atgaacaaaa
     4201 agctgaattt gaaaaagcag gtgcagatta cgcagcacaa aaagcagaat gggaaaaatt
     4261 aaaaaatgca gaaactgtca cagctgacgg caagcatttt gaattagctg caaacatcgg
     4321 aacaccaaaa gatctagttg gtgtccataa taatggtgga gaagcagttg gtctgtaccg
     4381 tactgaattc ctgtatatgg attctccaga cttcccaaca gaagatgacc aatatgaagc
     4441 ttacaaagct gtattagaag gtatggaagg aaaacctgta gttgttcgta caatggacat
     4501 cggtggagat aaagaactac catatctgca attacctcat gaaatgaatc cattcttagg
     4561 atatcgtgca ttgcgtatca gtttatctga acaaggcgac gaaatgttcc gtacacaaat
     4621 gcgtgcattg cttcgtgcat ctgttcatgg taatttacgt atcatgttcc ctatggtagc
     4681 tacactcaaa gaattccgcg cagcaaaagc gatctttgaa gaagaaaaac aaaaattgat
     4741 aagtgaaggc aaggaagtat ctgatacgat ccaagtcggc atcatgatcg agattccagc
     4801 agcagcagtc ttagcagaca agtttgcaaa agaagtagat ttcttctctg taggtacgaa
     4861 cgatttgatt caatacacaa tggcagcaga ccgtatgaac gaacgcgttt cttacttgta
     4921 ccaaccatat aacccatcaa ttttacgttt gatcaaaaat gtaattgatg cagcacacgc
     4981 tgaaggaaaa tgggcaggta tgtgtggaga aatggctgga gatcaaacag ctgttccatt
     5041 gctagtaggt atgggtctag atgagttctc aatgagtgcg acatctattc ttaaaacgcg
     5101 tagtttaatg aaacgcttgg acacagcgaa aatggctgaa cttgctgatc gtgcattgaa
     5161 agaatgcgat atgatggaag aagtcgttga acttgttcat gaatatgtaa aataaacaac
     5221 gtatcaaaag ctggtgtggc ttgtaaaaga gctacgccag tttttttgtt cttttttaag
     5281 aaaacgtttt tgtcagtaag aatagacaaa aatagggctt aagtttgagt gacagtttgt
     5341 aggctatgtt atactatata agtgaagggg gacgtaaggt gaaggtatta ttatattttg
     5401 aaagtgaaaa gatgcttgca aaatctggaa tcgggcgtgc gcttgatcat caaaaacgtg
     5461 ctctgacgga agtcggtatc tcttatacac tagatgaaaa agaagattat gacatcctgc
     5521 acatcaatac atacggaatc aatagtcata atatggtcaa taaagccaga agagaaggaa
     5581 aaaaagttgt ctatcatgcg cattctacag aggaagattt ccgaaattcc tttatcggat
     5641 cgaaccagct ttctcctatc gtaaaaaaat atctggtagg attgtaccaa aaagcggact
     5701 atctgatcac gccaacacct tattcaaaac agttgttgga aagctatggt atccgtgtgc
     5761 cgattcaagc tatttccaat gggatcgact tagagaaata tcaccctgat ccaatgaaag
     5821 aacaaaagtt cagagaatat ttcaaactgt ctccggatca aaaagtaatc atttgtgtcg
     5881 gtttgttttt cgaacgtaaa ggaatcattg atttcgtaga aattgctaag aagatgcccg
     5941 aatacacatt tatttggttt ggtcatgttc ccatgtactc gattcctcgg aacatccgga
     6001 aaattgtaaa agaagatcat cctgaaaatg tcctgtttcc tggttacatt cgtggagaga
     6061 tcatagaagg tgcgtattca ggtgcagatc tattcttttt cccttcttat gaagagacag
     6121 aagggatcgt tgttttagaa gctttagcaa gcaaacaaaa cgtacttgtt cgagatattc
     6181 cagtgtataa tggctggtta gaagatagca agaactgcta tatggggaag acaaacgaag
     6241 aatttatccg actgatccaa cagattacca atcaagaact accgagtacg acacaagccg
     6301 gttatcaaac agcaaaagag cgaagtatca ataaaatcgg tgaagaactt aaaaatgtat
     6361 acgaaagtgt actgaatgaa aaagtctcta gcaaattaaa aaaagtaaat caggtcaagg
     6421 actagtttgg aggcgaaaaa catgaaaatc ggctttttta ccgataccta ctttccgcaa
     6481 gtcagcggag ttgctacatc gatcaaaaca ttaaaacaag aattagaaag acatgggcat
     6541 gaagtctata tttttacaac gaccgatcct aatgcggata agttggaaaa agatgtgatt
     6601 cgaatgccaa gtgtaccatt tatttctttc aaggatcgaa gagttgttgt tagaggcatg
     6661 tggtatgctt atttggttgc caaagaacta gaactagatc ttattcatac ccatacagaa
     6721 ttcggagcag ggatacttgg gaagatggta gctaagaagt tgaaaatacc tctgatccat
     6781 acttaccata cgatgtatga agactacctt cattatatcg gtaaaggaaa agtgatcagg
     6841 caatcccatg taaaatactt gtctagatta tttgcaaatc atacgacagg tgtagtttgt
     6901 ccaagtgaac gtgttattga tacattacgc agctatggtg taatagcacc gcttcgcgtg
     6961 attcctactg gtatcgacat tgaaaaattc aaacgttctg atatcactca gcaggatatc
     7021 agcaaattaa gggagtcact aggtcttcag gaaaaccatt tgatgattct ttctttaagc
     7081 aggatttctt atgagaaaaa tatccaagcc ttgattaatg ggtttcctca ggtcatctcc
     7141 gcttatccta acgccagatt agttatcgta ggaaaaggac cttatgtaga agagttagag
     7201 gaattgatct cagaactgca gttagaagaa tttgtgcagt tcacaggaga agtggacaac
     7261 aatgatgtag cactttatta taaagcggca gattattttg tcagtgcttc tacttcagaa
     7321 acacaagggc ttacttatac agaagcgatg gcagccggaa caccttgtgt cgtggaagga
     7381 aatgcttatt tgaaccgatt gttcgatcat ccttcattag ggaaaacttt tgaaaccgac
     7441 gaagactttg caccagcact tattgattat atttccagtg aagttcctaa agatccaaaa
     7501 gttttagaag ctaaacttta tgatatttca tctacacatt tcggcgaagc tatgatcgaa
     7561 ttttatcaag acatggttgt ttatcatgaa gaagagatgc gaagaaaaga agaagaagac
     7621 tcgattgaac gaatcaaaat aaaattaaat tcatttaaaa gataaaattc aaacattttt
     7681 tatgaaaaaa tccttgtttc tagcgtatac tagcatagaa gcaaggattt ttgattttta
     7741 ggaggtcgaa aaaatgaaac ttggttttat cggaacaggc gtcatgggaa gtgctgtcgc
     7801 acgccatcta ctagaagcag gacacgaagt agctgtctat aatcggacaa aagcaaaagc
     7861 tgatccttta gtaacagaag gagctatctg ggcagataca ccaaaagcgg tagcagaaca
     7921 aagtaatatt ctctttacaa tggtaggtta ccccaaagat gtagaagaaa tctactatgg
     7981 acagtcgggg attttttctg cagatatctc aggacatatt ttagttgatt tgactacaag
     8041 tacaccaagc ctggcagaaa aaattgcaaa aacagcaaaa gaaaaaggcg cagatgcgtt
     8101 agatgcaccg gtatccggtg gtgacttagg agcaaagaat ggtacactga cgatcatggt
     8161 cggtggagaa gaagccgttt atgatcaagt actgccttta ttcaaagaat ttggtacgac
     8221 atttacattg catggttctg ctgggaaagg tcagcataca aaaatggcta atcagatcat
     8281 gattgcaggt acgatgacag gaatgacgga aatgcttgtc tatgcacaaa aaaacggact
     8341 tgatttaaag aaagtgattg agacgctttc aggtggaagt gcagctaact ggtctttaag
     8401 caattattct cctcgtattt taaaagaaga ttacacacct ggtttttttg tcaaacattt
     8461 tatcaaagat ttaaagattg cattagaaga agcagaaaaa atggatcttg tattgccagc
     8521 aactacccaa gcattgaaac tatatgaaga gttggcagat aaaggttttg agaatgacgg
     8581 aacccaagct ttgatcaaac tttggtggcc tgaaggaaaa ataccagaaa aaaaatcata
     8641 aaaaacgagt ttttttagag aaaatcaatg caagtaggct atgcttttga tacaatataa
     8701 cagaaataag aaaaggagga cctccgatga cacattcaca attagtcgca atcataaaac
     8761 gactggaggc tatggtcgag tcagcagata atgaactgca agttcgccgt tttgaaaaag
     8821 aaggcgtaga gaagtgtaca gtaacttatg ataaagcgac cgatacattt gaattaactg
     8881 aaacagatac tcatcagcaa tacgaatttg ataatatcga tatcgtagcg atggaaatct
     8941 atgacttaat ccaataaatt ttaggataat tgacatttga agttgaagat gcctaaaaga
     9001 agccgagaag caactaattt agttgctttt cagcttcttt tttgtttaac agttcaattt
     9061 caaactgttc tttttctaat tttttgcggt aactgatcaa gatttgtaca ggatcgattt
     9121 cttcttttga caattgacgt ttcaaagata aatgagcttc tttcttttca taatagaagt
     9181 caggagaaac gtcaccagag gtaatcattg tttctgcaag ttgaccataa caaacgaggt
     9241 agtgcttttt ctgactctca tctgctgcta atggcatgct gaaggtgagc gatggtgttc
     9301 ttaaaaactg cttttgttcc tggatccgtc taataaaata agtaactaat tcgatatctt
     9361 tttgaagcat accggctttt tcgaaattca actgatagat gaaatgttgt tgtctttttt
     9421 tcaagtaagt cagtagcttt ttatttcggc cttgaaacag cccttggagt tccttttttt
     9481 tctgttcaaa agtcatttcc tgtaggtcag gaagctggac tcgcaaagct aacaaactaa
     9541 tttcagacaa ccaaggcatt tgataagttt cttccattat acgcaaaata gacggcattt
     9601 ttttatagag acgaaaaggc ccgtatgttc gagccgtagg agtatctgtt aatacaaatc
     9661 ccttattggt aatatgtaca taattataat tactgaaata attcatttgt cggttataaa
     9721 aaggacggta atgttggatc aattgacact cgagcaataa agcatctaat tccgtgtcta
     9781 ctacgacgaa atcaaaatct gttatatgat gaatca
//
LOCUS       DACEXQ010000088         9753 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.088, whole genome shotgun sequence.
ACCESSION   DACEXQ010000088 DACEXQ010000000
VERSION     DACEXQ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9753
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.088"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(45..779)
                     /locus_tag="IXH04_001528"
     CDS             complement(45..779)
                     /locus_tag="IXH04_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ1852137.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1106..1516
                     /locus_tag="IXH04_001529"
     CDS             1106..1516
                     /locus_tag="IXH04_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAQ1852138.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1509..2210
                     /locus_tag="IXH04_001530"
     CDS             1509..2210
                     /locus_tag="IXH04_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAQ1852139.1"
                     /translation="MTEFFVNTLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2210..3796
                     /locus_tag="IXH04_001531"
     CDS             2210..3796
                     /locus_tag="IXH04_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ1852140.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3815..4036
                     /locus_tag="IXH04_001532"
     CDS             3815..4036
                     /locus_tag="IXH04_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAQ1852141.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4033..4794
                     /locus_tag="IXH04_001533"
     CDS             4033..4794
                     /locus_tag="IXH04_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAQ1852142.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5174..5683
                     /locus_tag="IXH04_001534"
     CDS             5174..5683
                     /locus_tag="IXH04_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ1852143.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5758..6297)
                     /locus_tag="IXH04_001535"
     CDS             complement(5758..6297)
                     /locus_tag="IXH04_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAQ1852144.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6437..7048)
                     /gene="rpsD"
                     /locus_tag="IXH04_001536"
     CDS             complement(6437..7048)
                     /gene="rpsD"
                     /locus_tag="IXH04_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAQ1852145.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7364..8161
                     /locus_tag="IXH04_001537"
     CDS             7364..8161
                     /locus_tag="IXH04_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAQ1852146.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            8189..8824
                     /locus_tag="IXH04_001538"
     CDS             8189..8824
                     /locus_tag="IXH04_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAQ1852147.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8844..9617
                     /locus_tag="IXH04_001539"
     CDS             8844..9617
                     /locus_tag="IXH04_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1852148.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
BASE COUNT         3022 a         1750 c         2042 g         2939 t
ORIGIN      
        1 ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc cataaataat
       61 aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac tggtacccat
      121 gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg tacaagacgt
      181 ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag tgaagcaact
      241 aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc ttggaagaat
      301 ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa gaattcattg
      361 acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc taacacatgg
      421 tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat caattgtcca
      481 atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca agggaataga
      541 ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct gattccacca
      601 aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag catccagtca
      661 ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt acctagaacg
      721 attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga gtaatccata
      781 aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg aaaagcatcg
      841 gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat aaaaaaatga
      901 attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt taaaagttct
      961 ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt tttctcaaaa
     1021 attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta gtaaaatgaa
     1081 agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca ttattatttt
     1141 cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc ctggaagtat
     1201 cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc ctgaaaaaat
     1261 cggagcaact ggtgattttt tattgaataa tctagccatt ttatttgttc ctgctggtgt
     1321 aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac tgggagcagt
     1381 cgtcgtgtgt tccatcgtca cgatggttgc agcgggaaaa acggcagaag gtgtagaagc
     1441 tttgctgggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg aaatggagga
     1501 gaatcaaatt gactgagttt tttgtgaata cattatttgg cttaagtttg actttaggag
     1561 tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg aatcctctcg
     1621 tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc tataaagact
     1681 ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg gcacttgcca
     1741 tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta atggctgcga
     1801 ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac ttcttctctc
     1861 ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct atttctgtcg
     1921 atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt gtctctacag
     1981 gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc gaaagtccag
     2041 ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat tggtacagca aaagcaatcg
     2101 agattggtga aatagaagga atcatttccg gacttgcgat atgcgtcaac ggaatattga
     2161 ccgttttatt gctaccgtta ttctttcagc cttttgctgg cttgttttga tgcgaaaacc
     2221 actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga aagacgtgta
     2281 tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga ttctcctcct
     2341 agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag ttgttcagct
     2401 ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc agcagtttag
     2461 cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact tttatgatac
     2521 gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta cgctgaaatt
     2581 ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat ttgcgcaaaa
     2641 gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac agcaactgca
     2701 aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag aagaaatcat
     2761 tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct ggccatttga
     2821 aacgatttct caaatggacg taaagaatat ggcagaacat atcatggctt tttttaatgt
     2881 tcgtctgaac gagatcaaaa aaagacgatt ggaatatatg ctaggtgcct tttttttaag
     2941 aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga tttctgacaa
     3001 tctgttgttc cagcgatttt gccaggtcat ggagccggta tttcctaatt attttcaagt
     3061 agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat attatagtga
     3121 tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac cgccctttac
     3181 agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg ggctgccagc
     3241 agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt tcgcctatct
     3301 ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta atcatttagt
     3361 tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta gacacaaaca
     3421 tcccaatcat ctggcattta aaaatcatgc gtttttgatg gggcggtatc tgactgtgtt
     3481 taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt taatgacaga
     3541 tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt tccgtaatga
     3601 gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc ttctgatttc
     3661 gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg tgactgaaga
     3721 actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc aaaaacaaaa
     3781 actattaaaa caataacaaa gaaggagaaa aaagatgaca tttgaagaaa ttttaccaga
     3841 attgaaaaaa ggtgcaaaga ttattcgtaa aggatggagt ggatttgaac tttatgttgt
     3901 tctcgtatcc gatgatgaat ttgatggctg tccggtgacg ccgtacttat tgatcaaaac
     3961 aagtgacgaa ggtttttcca gcttcgctcc tacagtttgt gatattttag cagatgattg
     4021 ggcgatcgta gaatgaaaaa ccagtatcca gatctagcta aacaggttgt atttatcaca
     4081 ggagctgctt ctggaattgg tgctgcgcaa gcccgagcgt ttcttgaaca agatgctttt
     4141 gtatttggat tagatcaaaa gttcggtaaa atggaagaaa tttctttgcg atttcctgaa
     4201 acttttgcct acgcactagg tgatgttcga caaatgaaag aactccaaca agcggtatcg
     4261 aaatgccaaa atcattttgg tgaagttacg attttactga atacagcggg gatattggat
     4321 gcctacaaac ccttgatgga aactgacgaa tcgctgtggg atctgattta tgagacgaat
     4381 gtgaaaagca tgtatcagct gacaaaaatc atccttcctg atatgatccg ccaaaaaagc
     4441 ggaacgatca tcaatatggc ttcgattgct ggcttgattg ctggtggagg tgggatcgct
     4501 tatacaagcg cgaaacatgc gattgtcgga tttaccaaac aactcgctct ggattacgca
     4561 ggacagggta tccgagtaaa aggaattgct ccaggagcca tccaaacacc aatgaatgcg
     4621 gctgattttg aagggaaagg tgagatggca gagtgggtgg caaatgaaac accagtcaag
     4681 cgatgggcac agccggaaga agtagctgag ctgacgttat ttcttgcctc tcctcaagca
     4741 agctacatac aaggaacgat catcccaatc gacggcggat ggttgttaaa ataaccttgt
     4801 gtttttttca agcttctgta tgataaaact aaagagagaa acaacggttg atgataaaaa
     4861 aagtcgatga ctgttcccga cattcgatgc cgaacagcca gttcaaaaaa ttctttttaa
     4921 taatcgttaa tattcgaaac taagcggctt ttcacgacct cttgatataa acgatattca
     4981 aaagcttgca actgcggtaa caagtccagc caaatgaaaa atataaggag ctatttttat
     5041 ttgaccattc ttattacttg ttgcagggca gctttttcac taccttctgc gacacttcag
     5101 ttggaatttt cttcccatcc tttggtcgcg gtcattacca agataaaatt atcttgcaaa
     5161 ggagaaatag aacatggaaa aaaataatac ccgttcagcc aaacgctgga caacgacaga
     5221 tgtgactcgg atggcgctag tcactggttt atatgtggca gtgacgctgg ttttgtctgt
     5281 ctttagcttt ggagtgattc agattcgtct ctcggagatg ttcaactatc tgtccttgta
     5341 taacaaaaga tacatcgtcg cggtaacagc gggagtagct ttggctaata ttgcttctcc
     5401 tcttggactg atagatgttg cagtaggaag cgtcagcacg ctgcttgtat tgcttatcaa
     5461 ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa atggccgtga cagcatgcgt
     5521 atttgctttt tccatgttta cagtagctgg tcagctaacg attttatatc aactaccttt
     5581 tttcttgaat tggtgggtga ttgctttagg agaattggca tcaatgattg tcggtgggat
     5641 aattatttat tggatcggta aaaaagtgga tttgtcaaaa taaagatctg tttaatttaa
     5701 aatagggcag catggaagag actgaatgtt gagtcttctt ccatgctgct tttttgatta
     5761 atttgtatga agaaatcgat ttagccgttt tttcatcaag ataccgatat aagctgctcc
     5821 agttgccttg attgcttccg gcagtaaaaa cggcagtagt ccactggcaa atgcagttgt
     5881 aaaagtaaga tcaccactta ttttcagcca gatcgttccg cagatcaatg cagcaagaga
     5941 gcccattaga ttggcaaaga ttgcatataa gtaatgagca tccgttcttt cgatcatcca
     6001 accagttatc cacgtttgta agagaaatcc aatcaaatat ccgccagttg gcccaaataa
     6061 aacagctata cctccagtca tccctgaaaa tacaggaaga ccaatcaagc caagtaaaag
     6121 ataaactcca acagatatca atcctagttt tcttccgaga aaagttgctg ttaatccaat
     6181 agcaaatgtt tgccctgtca aaggaaccaa accaaaagga atggtcatct gagaaaggac
     6241 agaaataatc gcggcaaatt ccgcagcaag tatcaattct ttcaatgttc ggttcataac
     6301 gccctctttt cgttaactta atatttttat ttggttaacg aaattgtaaa acaaagtgat
     6361 tactttgtaa atagaaaata taaaaaaaga ccagctatcc aatagatagc ggtctttttc
     6421 aagaaagctt ttgaaattat aagctttggt tgtagtattc aacgacaaga gcttcgtcga
     6481 tttctggata tagttcgtca cgttctggta aacgagtcaa tgaaccttcc aatttttcag
     6541 cgtcaaagct tacgaatgct gggcgtccaa cagttgcttc tacagcttct tttactgtta
     6601 cgatgttttg tgatttttca cgaactgaga tcacttgacc cacttctacg tggtatgaag
     6661 ggatatcaac gcgtttgcca tcaactaaaa tgtgaccatg gttaactaat tgacgtgctt
     6721 gacgacgagt cgttgcaagt cctaaacggt aaacaacatt gtctaaacgt tgttctagta
     6781 agatcatgaa gttaacacca tgtttacctt ctttgatttt gctagctttt acgaacaagt
     6841 tacggaattg acgttcgttc attccgtaca tgtgacgaag tttttgtttt tcagttaatt
     6901 gcatgccgta ttctgattgt ttgccacggc tattaggtcc atgttgacct ggtgcgtaag
     6961 ggcgacgtgc taattctttt cctgtgcctg acaatgagat gcctaagcga cgagaaattt
     7021 tccatgatgg tcctgtataa cgagacatta aaatttcctc caatgaatat aagtttttgg
     7081 agtaaaataa tccgttgaaa aattcataat catatagttt gttcttcaat cttcaccttt
     7141 gcagccgcgg ttacacaatc gaacccgaga cggggcaaca aactagggat gagttattat
     7201 ttttctgctg cattatttta cacaaattac agtatatgat agatcatcca cgaatgtcaa
     7261 gataattagt acataaaaac gctttacaaa cctaggctgt ctttataaaa tgagagggta
     7321 tgtgcaaatg gaaaaaagcg aaggaagttt aaggagaaaa tcaatgaatc cggtatcacc
     7381 attatttgag aaaatcgatg cttcaattga aaagaaggcg aatctttttg aaaacagctt
     7441 ttcacgttac gctgttcgag cggttctcgc ctgtttgttt ttgactttag gaacggctgt
     7501 ggcatttgcg atcgcgatga agggggaaga tatcgcgcat ggtctaggaa agattttata
     7561 cgcatttatg ttcagctggg gtctggtcat gattctatac atgaacgccg aattaggaac
     7621 atcgaatatg ctttatatga ccgtgggtgt ttttcggcag agacttgctt taagtaaggc
     7681 agccaagatt ctatttgcct gtattttttt caatatgatt ggcggtatat tatgtggatt
     7741 tctaatttct atgactacgc catttcaaga tttaacgttg gacaacttta tgttagaatc
     7801 aatagcagta aaactagcaa aaccttcagc aacgattttg gtggaaggga tgtttgcgaa
     7861 tatcgttgtc aataccgctg tactgatcag catgcggatg aaagaggatg tcggaaaagt
     7921 gatcacagtc gtattcatca ttttcatttt tgctttttta ggatatgaac acgtgatagc
     7981 gaattttcct gcctttacgc ttacttactt tgcttctcac ggacagatgg atggaatgac
     8041 agtcagcaat gtgttacata atttgttttt tgctttggca ggaaattata ttggtggcgg
     8101 tttagtaatg ggactgggat atgcttggct gaatcagtca aaatcatcct atgtggacta
     8161 aaaagtcgtt cattaaaggg gaagtgccat ggaagtttta gtttttccaa aaaatgagga
     8221 aaaagcgtta caaatggaaa agtacatgaa aaatcttttt tcctttgctg gagtacctaa
     8281 gccggaacgc gctcttttac aacaaccgtt actggatgag agcaaaaatt tatctatttc
     8341 tgaagtttta gcttttgttc attattatta tgaacaaatc gaaagagagt atcagtatgt
     8401 agcgatcgat ttagtccaga aaaacatcaa acggcttaca tttgaagact tagtcgtatt
     8461 gttgccacta gttcagaaaa aagaatggtg ggacagcatc gactcttgga gaaaagtata
     8521 cagcacatgg tgtaaagcct acccagagca gcgtgaagaa gtctttcaat tgtttgaaaa
     8581 aaaggaagat ttttggctac gtcgaatagc aatcacgttg caattaggtt ataaacagca
     8641 aacagacaca ggactattga tccgagcaat agacgaagat cgtcaaacaa aggaattctt
     8701 tatccaaaaa gcaattggtt gggcattacg cgattatagt aagaccgatg ccgattgggt
     8761 aaaaagacag ctgacaacag atttgagtaa gttagctgtt cgagaggcaa gtaagtattt
     8821 ataagaaagc gagacgacaa gagatgagac gcaaattatt tgcttttgat attgatggaa
     8881 cgttgttaga ttcaaacaaa caagctttag atagcacaag agaagcattg gaaaaattac
     8941 gcaaagccgg tcatctggtc acgattgcta cagggcggag ccgatttcat gcgcaagaga
     9001 tcattcgcga tcttgatttt acgaactaca ttttatgtaa tggagcggcc ggttttttag
     9061 accatgaaca agtatataag aatttactag atcgcgacca gttggatgcg ttcgttcaag
     9121 aagcgcatca tgagcagatc gatacagctt ttgtcagctt agacaatatc aagcgttctt
     9181 cttccaatga tgtaacggtg atggaagaag cgatgcattc tttcggtgct gtcttgcctg
     9241 aattagatca gttttttgca gaggaacaag aaatatacca agcgttagca tttttcgatc
     9301 aaacatatga tgggaagttc tcttcatatg ataaaattcg atttgtccgc tggcatgaga
     9361 acagtgtaga tgtcgtacct aaaaatggtt ctaaagcagc gacaattctg cacttagcag
     9421 aacaagtagg catccctcat gaagacatca tcagctttgg cgatggacaa aacgaccgtg
     9481 aaatgttgcg catgtcagga attgggatag ctatggggaa tgctgtgcca gaagtacaag
     9541 cagaagctaa gatggtcact gatacaaacg atcaggatgg aatttggaag gcattgaaag
     9601 agttaagcgt gatttaaaag aggttgtgaa aaagctgttc tgcatcaagt aataagccca
     9661 gccaaacaaa aatagctcct cctatttttc gtttttctgg gcttattacc gcagatgcaa
     9721 gctttcgaac accgtttaag gaagaggtcg tga
//
LOCUS       DACEXQ010000089         9747 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.089, whole genome shotgun sequence.
ACCESSION   DACEXQ010000089 DACEXQ010000000
VERSION     DACEXQ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.089"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            241..2712
                     /locus_tag="IXH04_001540"
     CDS             241..2712
                     /locus_tag="IXH04_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ1852149.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2815..4551)
                     /locus_tag="IXH04_001541"
     CDS             complement(2815..4551)
                     /locus_tag="IXH04_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAQ1852150.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSVFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4548..5252)
                     /locus_tag="IXH04_001542"
     CDS             complement(4548..5252)
                     /locus_tag="IXH04_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1852151.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5383..5886
                     /locus_tag="IXH04_001543"
     CDS             5383..5886
                     /locus_tag="IXH04_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAQ1852152.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            5846..6184
                     /locus_tag="IXH04_001544"
     CDS             5846..6184
                     /locus_tag="IXH04_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAQ1852153.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6205..7623
                     /gene="ffh"
                     /locus_tag="IXH04_001545"
     CDS             6205..7623
                     /gene="ffh"
                     /locus_tag="IXH04_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAQ1852154.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7735..8313
                     /locus_tag="IXH04_001546"
     CDS             7735..8313
                     /locus_tag="IXH04_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAQ1852155.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8317..8838
                     /locus_tag="IXH04_001547"
     CDS             8317..8838
                     /locus_tag="IXH04_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1852156.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(8917..9471)
                     /locus_tag="IXH04_001548"
     CDS             complement(8917..9471)
                     /locus_tag="IXH04_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ1852157.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
BASE COUNT         3197 a         1785 c         2007 g         2758 t
ORIGIN      
        1 tcgactaaat tttaccaaaa gtgagtctct ttttgagttg aaacgaagaa aaagtgttag
       61 tagaaaatcg ccttcgattt tccaccaaca caaaatatta tagaaccctt tttttttgct
      121 atatgacagg aatgaaaggt gtttgcaagc ttaatgtgag gaacactttc catatctgtt
      181 ctatactgga taggagaagt ttggaaatat ccagaaaacg aaatcaatcg aggtagccac
      241 atgaagaata aatggataaa tcgaagtaaa aaaaagaaag taacacaaag atcatcagat
      301 aaagtcatcc gtctttttcc atcgtataaa gaaggattaa cggcagatca agtcaaggaa
      361 cgaatagaaa aaggtgcagc aaataattca gtcgatccca cattcaaaac aaatcagcaa
      421 atcgtattgg aaaacatatt tacgtatttc aatttgatct ttcttatctt agccattttg
      481 ctttgtttag tagaatccta taagaacttg acttttttac cagttatcat agccaataca
      541 ggaatcgcca tttttcagga aattcgatcc aaaaagatct tggacgaact aaatgtttta
      601 aatacagcaa aagtcaatgt catacgagac gggatagaac aaacaataga tatagaagac
      661 cttgtaatag atgacattgt tattttagag actggatatc aaattccagc agatgcagag
      721 gtcgtggaag ggaaactgca agttaatgag gctttattga caggcgaagc agatgaaata
      781 accaaagaaa taggtgatcc attaatgtcc ggcagtttca ttgtttccgg aaaagcttac
      841 gcccgattag acaaagttgg agcagattcg tatatttccc agctgacagt caaagccaag
      901 acaatgggag aaggagaaca gtcggagatg attgcttctc ttaatcaatt gatcaaatgg
      961 attgggatca tcattattcc tatcggtatc ctgctttttt ctcaaagtta cttttttaat
     1021 ggaaacacga tcaaagaaag tgtcgttgca atggaagcag cattgatcgg tatgatacct
     1081 gaaggactgt acttgctgac gacgattgca ttagcattaa gtgcgactaa actagcaaag
     1141 caaagagttc ttttacacaa tatgaaaagc attgagacat tggcaagggt aaatgtcctt
     1201 tgtgtagata aaacaggaac gatcacagaa aataaaatgt ctgttcaaaa aatgatcgtc
     1261 tctaaaaaac aagaaataat cagtcagtca gatcaattgg aagcatgcat tgctgactat
     1321 gcaaaagcga tggcaggaga caatgcaaca atggaagctg ttaaagcata ttttacaaaa
     1381 accactgatc aatcctatca ccatgttatt cctttttctt ctgtccaaaa attcagcagt
     1441 gtagcgttgg cagacaaggt gtatgtttta ggagcgcccg aaatggtttt gcgcgatcaa
     1501 ttgccggaat atgcggaaga gtttgtttct ttcgctgaaa aaggctatcg cgtgttggtc
     1561 tttggtgtgt acgatgggat tcttgaagaa ccagtattga cagaagcagt cacaccatta
     1621 ggctatattc tgattgctaa tcctatcaga aaagaagcaa gagccacatt tgattacttc
     1681 aaaaaacagc aagtagccat caaagtgatt tctggagata atccgctcac cgtatccaat
     1741 gtagctgtcc aagcagggat ttcacatgct gatcagtacg ttgatgcatc taaattatca
     1801 gaagaagaat atgaagcagc tgtcgaaaaa tatacagttt ttggacgcgt caagccagaa
     1861 caaaagaaaa tcttcgttca attgataaag cgtaacaata cagtagctat gacaggtgat
     1921 ggcgtgaatg atattctagc catgaaagaa gcagactgca gtatagcgat ggcttccgga
     1981 aatgaagccg ccatgcaggc ctcacaagtg gtcttacttg attctgactt ttcacgcatg
     2041 ccagaagttg ttgcagaagg acggcgcgtt gtgaacaata ttgagcgatc agccagcttg
     2101 tttttagtga agaatatttt ctcctttttg ctctcactgt tttcagtgat ctttgctctg
     2161 acctatcctt tagaaccatc acaaatcaca ttgattagtt tgtttactat cggacttcca
     2221 tctttcttgc ttgctctaga agaaaacaaa aagcgaatca gagggaaatt catcatgaat
     2281 gtgatggaaa aagctgtgcc aggtggacta acagatatga tcgtagtcgg tgcgttggtg
     2341 atctgcgggg tgactttgaa tctaaataaa acagatgttt ctacagcatc taccatgctt
     2401 ttgattgcag tagggttcct cgttctctat aaaatctgtt caccgctgaa taaattcaga
     2461 agtcagatca ttcttttctg tgctagcggt aacttttttt cggtcatctt tttgcataag
     2521 ctgttttcaa tcaccggtat ctcagctgta tcacttttgc tgttgagcat tctatttttc
     2581 tcggcagatt cgatttttag gcatttgacc acattggtag aatatttgtt tgaacgtaaa
     2641 aatgaaaaag agcaaaagcc gtttagtcgc gtagctaaaa aatttctatc gatatttaaa
     2701 cgagaaaatt gacgaaaagt atctcaaaaa agagtaaaaa agaacaacca ctcgtgtatc
     2761 tttcgaagca ggatgagtca tccaacttta aaacgcacga gcagttgctc ttttttacat
     2821 tttcggaatg gtaacggtga aagtcgaacc tgttccaaga tgactgtcta cagtaatcgt
     2881 accattaagg agttgcacgt agtcttttac aatcgctaac cctaatccgg tcccgcctga
     2941 atgtcggctt cgcgccttgt ctacgcggta aaaacgctca aagatccgtt cctgatcctc
     3001 aatgtcgata cctataccaa agtcttggac agaaaacgat agatcatctg ttgcctgata
     3061 gcggataata atctcgctgt cgctttttga atattggaca gcattttcaa tcagattttt
     3121 gataattgga taaaggatat ctgttttagt agtaaatggt tcgttctttc caatcaatcg
     3181 aatcgtcaac cgtttctttt cgatgattgg ctgataattt tgaacgattt gctggaaata
     3241 aggttctgta taaattgtct ggatctcgta agaattctcg ccatcttttg acagctggat
     3301 gatttcccga atcagtttat ctaaccggat cgcatctttt tgcatgatct caagaaaaga
     3361 agtcagagtt tgcgggtcat cttttgcacc atccagtaaa gtctcggtaa aaccaatcag
     3421 tgaagtcaca ggagttttta attcgtgaga gacgttgcca acgaaatcct tttgtagctt
     3481 ttctaaacgt cgcactttcg ttaagtcata cgccacccct agaatctgtc cggtaccttc
     3541 tgaattagta aagtagcgca aactcaagtc taatgtctgc cgaccttctg taagggtgat
     3601 ctcctgatgg atcagcggat gctcaggagt cacttgatga atcagctgaa tgagttttgg
     3661 ctcttgaatg acttctgtgt accgctgttc aggttgatag cttaccacgt ttaaatgtag
     3721 ctgcatcttt ggattaagca gtagtaaacg agaatcagca tcatcaatca ggaatacgcc
     3781 aatcatcagt tcgttcagta acgtatataa ttgctcttct gtggtagtac acgcccgata
     3841 catcttactc atttgttcgc tcaacgcatt aatcgtctga tacaattctt cccattgatc
     3901 gggtgtttgc atgattactt ccgcctgatt aggattttgc accatttttt ttagtacggg
     3961 cagcacggtt ttcaatggct ggtttttttg atagatcaga taatagatca ttcctgcaag
     4021 aacaaggaag aaagacagaa agaagaaaaa gacccaacgg cgaaaactct cagttctcgg
     4081 taaaaaaacg cttgtcggtt ctgcgatacg taaaatggct tctaactggc cacttttttt
     4141 gactggtaat gcgacatata aaagatcctc attcaaagta gtacttttgc gcaaagcgct
     4201 acctaggttc ccaccattca atacggcttt gatttcggga cgggagtttc gctgttcatt
     4261 caatgtttca ttgtttgtgt caaataagat ctttccagta gcatccatta aagtaatccg
     4321 ttcgttcgac tgatggacga attcttccaa agcagttttg ttttgcgctg cttggaggtt
     4381 ttcgacatct aattgccgga tcagcagttg tgcttttttt gtcaggtagc tttcttgctg
     4441 ttccacgatc tgctgttgaa aataatggga aatcaattgc cagccaccaa aaaacaaact
     4501 aactaaaaga ataagaaaga agagacgttc ataaattttt ttccatttca tcgtttgggt
     4561 tcctgaaact tatagccaaa tcctctaata gtcaccaagt actttgggtg ttttggatct
     4621 cgctcgattt tttctcgcaa atgactaacg tgtacatcta cgattcggct ttgtcctgcg
     4681 aagtcaaaat tccaaatgcg gtctagcaat gtgtctcggt cgatcacacg gtcttttcgt
     4741 ttcatgaaat aaacaagtaa ttcaaattct ttcggtgtta gttcaatcag atggtcgtct
     4801 acggtcactt gataattggt caaatcagca gtgatttgtc caatcgatag atattcaggc
     4861 tcagcttcat cttttgattg cttacgaggt tcaattcgtc tgaatattgc tttcatacgg
     4921 gctaatactt cgcgaggact aaatggtttg gtcaaatagt cgtcagcacc tatttctagg
     4981 ccaatgatcc gatccacttg gtcatcttta gccgtcagca tcaaaatcgg tgtttcattt
     5041 ttttcttgac gcagccgctg agtgatctcc atgccatcca tcgttggaag catcacatct
     5101 aaaatgatga aatcaaatga ttgttctaat gctaattcta atccttcgcc tccatctgtg
     5161 gcactggtta ccttgtagcc ttctttttcc aaattgaacg tcaagagcgt tacaatagaa
     5221 ggttcatcat caacaactag tatttttttc attcaaatct cctttaaata ggtttcattc
     5281 tgtttatatt ttagcataga atcagtacaa agggctattt tacaaaagaa ataaatggta
     5341 taataggaag gcactttgct gaatagaaag gaagagcagt caatgattgg aatcagtgca
     5401 tgtttaggcg gtctggcctg tcgctatgat ggacagaaaa aagaagtacc agaattgatg
     5461 gaactagttg aaactggaga agctgtcatg gtctgtccag aagtgatcgg cggattacca
     5521 attcctagag atcccgctga aatcattggt ggaaatggat tcgatgtgtg gaaagatcaa
     5581 gcaaaagtat ggacgacaag cggcgaagat gtaacggaag cgtacaaaaa tggcgcgatc
     5641 aaagcctatc aaaaattgca ggaaaaacac attgatctag tcatcatgaa ggaaaaaagc
     5701 ccttcatgcg gaacccattc gatttatgac ggaagttttt ccggaataaa aaaaaatggt
     5761 gccggagtgg ctactgctta ttttattcag caaaaaatga ctgtgctctc tgatgaagaa
     5821 tggctgaaaa ctagaggtga gcagaatgga aatcgaaaag accaatcgaa tgaacgcgct
     5881 ctttgaattt tattcgacct tattgacaga aaaacaaatg aactacatgg aattatatta
     5941 tgcagatgat ttttcattag gtgagatcgc ggaagaatac gaagtaagcc gacaagcagt
     6001 atatgacaat atcaagcgga caagcaagat cttggaggat tatgaaaaaa aactccatct
     6061 tttttctaat tatattgttc gagaacaggt attggaaaat atgactgcat atattacaga
     6121 taagtacccg gaagataaaa aactacttga atatgtacag caaattcaag caatcgaaga
     6181 ataaataaaa ggaatgaaga aaatatggca tttgaaagct taactgaacg tctccagcag
     6241 gcgatgggaa aaataagaaa aaaaggaaaa gtatcagaag ctgacgtaaa agaaatgatg
     6301 cgggaaatcc gccttgcctt actagaagcc gatgttaact tgcaagtagt caaagatttt
     6361 actaagcgcg tacgcgaacg tgcggtaggg gcagaagtgc tggaaagttt atcacctgca
     6421 caacaaatcg taaaaatcgt tgatgaagaa ttgacgatca cacttggttc tgaaacagca
     6481 gagttaaata aatcaccaaa aataccaact gttatcatga tggctggtct tcaaggggca
     6541 ggtaaaacga cctttactgg taaattagcc aattatttaa agaaaaatga aaatgctcgt
     6601 ccgctcctaa tcgcagggga cgtatatcga ccagctgcga tcgaccaatt gaaagtatta
     6661 ggtcaacagc tagacgttcc tgtatttgat atgggaactg aagtcagccc ggtagagatc
     6721 gtccgtcaag ggatggagtt agcaaaagaa aagaaaaacg actatgtatt gatcgatacg
     6781 gcaggacgtc tgcatatcga tgaaacattg atggatgagt tgaaacaaat caaagaattg
     6841 acgcaaccaa atgagatttt gcttgttgtc gatgccatga ctggtcaaga tgccgtcaac
     6901 gtagctgata gcttcaacca gcaattaggt attacagggg tcgtcatcac taaacttgat
     6961 ggggatactc gaggcggggc agcactatcg atccgctcag tgacaggcgc accaatcaaa
     7021 ttcatcggtt ctggtgaaaa attgactgac ttagaagtat tccacccaga tcgtatggct
     7081 agccgtatcc ttggtatggg ggatatgctg acattgattg aaaaagcgca gcaagactac
     7141 gacgagaaaa aagcggaaga acttgcacag aaaatgcggg aaaattcatt cgatttcaat
     7201 gactttattg aacagcttga tcaagtgatg ggtatgggcc cactggaaga tctaatcaaa
     7261 atgatcccag gaatgaatca agtccctgga atcgaaaacg tcaaggtaga tccaaaagat
     7321 gttgaacgca aaaaagcgat ggtttattct atgacgccag cagaaagagc aaatcctgat
     7381 ctgttgaacc caagccgtcg tcgccgaatt gctgccggtt ctggaaacag tgttgttgaa
     7441 gtgaatcgaa tgatcaaaca attcaaggaa tcaagaaaaa tgatgcagca aatgtccaaa
     7501 ggggatatga acatcccagg tatggaccaa atgtttggtt caggcgtcaa aggaaaacta
     7561 ggcaaaatgg cgatgaatcg aatgatcaag aaaaataaga agaaaaagaa aaaacggaaa
     7621 taagatgaaa aaaacagctt cggtacagac tgaaaatgtc tactgccgga gcttttctat
     7681 cagcaatttt tattctttac taatacaatt actctaaacg aagaggtggc acagatgaga
     7741 tgtccgtgga gtgaacaaac agaaagtatg cggcaatatc atgatactgt ctggggtgtt
     7801 cctgaatatg atgaccaaaa gctgttccga aaattgatgc ttgatattaa tcaagccgga
     7861 ttgagctggc agacgatttt aaataaaagt gaagcattcg atgaagcata tgatcgattc
     7921 gatatcaaaa aagtcgcaaa ttatggagaa acaaaaatca gtgaactaat ggaaaacaaa
     7981 ggaattattc gtaaccgaag aaaaatcgaa gcagccgtcc aaaacgccaa agcagtcttg
     8041 aaaattcaag aagaaatcgg cagtttttca gagtatcttt ggtcgttttc taatggaaaa
     8101 atcgttgatt cttcttatca ttcacaagaa gaagtcccaa caacaagtcc tctttcagat
     8161 gaattagcaa aagatctaaa gaaaagagga tttaaattta tcggaagcac aacaatctat
     8221 gcttttttag aagctgtcgg gatcatcaat gatcaccttg acagttgttt ccgcaaagaa
     8281 gaggtcgtac gtatcggcag aaagggcgaa taaagcatgc gaaaaatcat attttatggt
     8341 gctataagtt tagatggcta tttggcaacg aaaacggatg atcttcaatg gctttttgac
     8401 actccaacag gtaagcaaac tacttatgaa gctttttatg aaacaatcga tacgaccatc
     8461 atgggcagaa aaacttatca ggaagcaaaa aaatatttgg agaccgaagc aatttatcca
     8521 gagaaaaaga atttcgtttt ttccacccac gcacaattac aattgccaga tgctgaggtc
     8581 gtaaacgaag atcctgttgc ttttctaaaa aatctacaaa tgacagaagg aaaagatatc
     8641 tgggttgtgg gtggcggtcg attgatcaaa ccgctattgg aacatcaaat gattgatgaa
     8701 tggtatatcc agattacccc tgtattatta ggggacggga ttcctttatt ccaaaaagga
     8761 acatatgaag cgcgttttga aatgctagac acgacacgat tcggtgaatt tatcgaattg
     8821 cattatgtac gaaagtagaa gtaaataaaa taaaaaaggc aactatatgc agaaatgtcc
     8881 tgtacatagt tgcctttttt attaccgtta tgctttttac tctgttaatt tgttagaaaa
     8941 atctagatac gcatctaaaa tcgtttggaa gaaacttaat gttccttcaa cgatattacc
     9001 attttcatca attagattcg caacgtttcc gatatatgct tccggttgct gcaaggttgg
     9061 cacattcaaa aagacaagtg attggcgtag atggtggttt gcgccaaagc cgccgatccc
     9121 acctggtgat gcagtaacga ctaaacctgg tttatgatcc caaacactgt gtccataagg
     9181 tcttgagcct acatctaaag catttttcaa tactgctggc acagaacggt tgtattcagg
     9241 agtaacgaaa acgacaccgt ctagtcccct cacttcctca cgaaaacgag tccattcagc
     9301 aggagcctgt tctggagttt cgagatcctc attgtaaaat ggcagatcat cgatttttac
     9361 aaatacactt tcgtaccctt caggaagcaa tttcgcaaaa gcttctgcta cttttttgtt
     9421 gtaagaatct tttcttaaac taccaataaa aaatccaatt ttttttgtca tacgattttt
     9481 cctccttcaa actatctata ataatcttac aaaagataag taactggagc aaataaaaac
     9541 acttactgac aaaagctaca tagaagatag attacttatt tttaacaaaa aagaagtcta
     9601 aaatcttcta ttttctaaaa aaaatcgaat ttaatcaagc tgtaaagaaa aaaagcttta
     9661 cagggtaaaa aaaaactgtt acactacatc ttgtgaataa aacaatggag gtggaaataa
     9721 ataatggcag ttaaaatccg tttaaaa
//
LOCUS       DACEXQ010000090         9737 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.090, whole genome shotgun sequence.
ACCESSION   DACEXQ010000090 DACEXQ010000000
VERSION     DACEXQ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.090"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(74..1825)
                     /locus_tag="IXH04_001549"
     CDS             complement(74..1825)
                     /locus_tag="IXH04_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1852158.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAAFLQYLFRYIWRTNIWGSAARLERELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            complement(1967..2191)
                     /locus_tag="IXH04_001550"
     CDS             complement(1967..2191)
                     /locus_tag="IXH04_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAQ1852159.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            2442..4028
                     /locus_tag="IXH04_001551"
     CDS             2442..4028
                     /locus_tag="IXH04_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAQ1852160.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            complement(4094..5869)
                     /locus_tag="IXH04_001552"
     CDS             complement(4094..5869)
                     /locus_tag="IXH04_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAQ1852161.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(6080..6982)
                     /locus_tag="IXH04_001553"
     CDS             complement(6080..6982)
                     /locus_tag="IXH04_001553"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAQ1852162.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     ISMFKAQAFDPEVFPELLAEEVTL"
     gene            7261..9606
                     /locus_tag="IXH04_001554"
     CDS             7261..9606
                     /locus_tag="IXH04_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ1852163.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKHFSKNEIITTYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGNA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
BASE COUNT         2968 a         1950 c         1824 g         2995 t
ORIGIN      
        1 agtcgtttga tgatcttctt ctgttcttta agtggaatcg attttgtcca ttccgattga
       61 taattttcat ccatcaggcc tcgcctcctt ctatcttcgc ttctaattgc tgtttttccc
      121 acattcgttc ataccagcca cctaatgcaa gcagctcgtg gtgggttcct cgttcaatga
      181 ttttcccttg atcgacaaca atgatttctt ttgcatgcat cacgctgctt aaacggtgag
      241 ctgcaatgat ggttgttttc tcttttctta tttcttttaa attatgcaag atagcttctt
      301 ctgttttggc atctactgca gaaagtgcat catctaagat cagcaattca ggatttttga
      361 tcaatgctcg agcaatcgaa atcctctgtt tctgtccgcc agataaagag actccacgtt
      421 ctccaactaa ggtatcataa ccttttggca attccttgat ttcattgttg ataaatgcta
      481 aagaagccgc atgctctact tcttcttctg aaagttttgg attagaaaaa cggatattat
      541 ctcgaatggt catcgaaaac aggaagtgat cctgaggcac ataaccgatt gatcctaata
      601 aagcattcaa tgaatattct ttgatcgaac gtccgccaaa ttggattttc ccttgataat
      661 catcgtattc acgcatcagt aatttgatga ttgtcgtttt tcccgcacca gttttcccaa
      721 caatacctaa tgtttctcct tcttggatcg caaatttgat ttgttctaat gctggtgatt
      781 cttctttcgg atagttaaaa gaggaaatag ctacattcag ctcgcctttt gccggggtct
      841 gcactgcacc cagttgttcc acgatatgcg tcttttcatg caaaagttca ttgacgcgat
      901 cataagaagc atttcctctt tccaatacgt tgaacaatcg accaatcgca aacatcggcc
      961 aaacaagcat accgatatag ctaataaatg agaccatctg cccgattgag atcgtgcctt
     1021 ccaagacata ctttccacct aaaataattg ttgcaacata agacagaccg ataatcaagg
     1081 taatgaatgg atcaaataag gagtctaaaa aattgactcg tttgttttta ataattgcat
     1141 cttcaatctt ttcttcaaaa tccgcgagat cttctttttc ttgtccaaat gttttaatga
     1201 ctttgatccc agtgatactt tcttgtgtct tatcattgat actagaaaaa gcttcttgcg
     1261 aatcacgaaa agcatcgtgg agttttgacc ccagcactcg tgaagtaacg gctagcaaag
     1321 gcagaggaat cagcgcaatc agtgttaagc gccaatcaat aaacaaaatc atcgcaatga
     1381 tcgttgttcc gcccgtaatc actgaatctg caaaggtcaa gattccagct cctgctacat
     1441 tctggattgc attcaaatca ttcgttgcat gagccatcaa atctccagtc cgatattttt
     1501 ggtaaaagag attgtccatt tctgtaaaat gtttgaatag ctgctgccga agttctctct
     1561 ccaaccgagc tgcacttccc caaatattcg tccgccagat ataacgaaaa agatattgta
     1621 agaaagctgc aaggatcaat aataagatcc accaggaaat gatctttatt gataaattct
     1681 tatcggcgat ctcatcaatg atgatcccaa tcacttttgg aggaactaac tgtaccaagg
     1741 ccactgcgaa tagggagaaa atcccaatta aataatgttt cttttcctga cgaaaaaacc
     1801 aacctaattt tctaaaaatc gacattttac tcctcctttg tctttatcaa gagcatagga
     1861 ttgtctgtgc ttgtcttttt tgctgaatag tctgaatagt ctaacaaatt acgaaataaa
     1921 aaacggactg aaaaaatcag cccgcaaaaa gtctttgggt cagaacctat tttttccctt
     1981 ggttcttcat ttgctgcatc atctggcgaa ttttcttctc agatggtttt tgccccattg
     2041 acatcatcat tgatcgcaac atgtcctcat taacgggagg atttttttct aaatagtctt
     2101 tcatatattt acgcgctagg aaaaagccac ctactgcgcc gattagcaaa gcaataatag
     2161 caataagtac gacaatacct gttgatacca tataaatctc tcctttctat ttccgaattg
     2221 ctctcaatga tttattttac taaagtttat tgaaaaaaga aaggcgtatt ctttagtttc
     2281 atgcaaaaaa tagtggtttt tttagagaag aacaaaattt ctttcattaa ccagcaagac
     2341 acaccacgaa aaaaaattag gcataccgaa tctttttctt taattttttc tttctgaggt
     2401 gtagaatgta atcgtagata aataggaaag gtggtttttt cttgaataat caatccaaca
     2461 aacaatttca taagcatcga cttattgaac atacaaatgg tttatcttta gctgaagtaa
     2521 atggaacagt ggatgtacca aagggaaaaa gtttttttaa aacacttctt gcttactctg
     2581 gaccaggtgc attagtagca gttggttata tggatccagg taactggtcc acttcgatca
     2641 ctggtggaca aaatttccaa tatttattga tgtcagttat tttgatgtct agtctaattg
     2701 ctatgctttt acagtatatg gcagcaaaat taggaatcgt gacccaaatg gatttggccc
     2761 aagcaatccg agcacggaca agtaagacat tgggtgtcat tttatggatc ttgactgaat
     2821 tagccattat ggcaacagac atcgcagaag tcattggtgc agcaatcgcc ctctacttac
     2881 tttttgatat tccattggtg attgccgtct ttattactgt attagatgtt ttcgttctct
     2941 tactactgac aaaagtcggg tttcgtaaga tcgaagcact ggttgtctgc ttaattttcg
     3001 tcattttatt cgtttttgtc tatcaagttg gcttgtctaa cccagattgg cacagtttat
     3061 ttgctggctt tatccctaac agtcatacat tctcagactc accggtcatc ggcggcgaaa
     3121 ctccgctgac tggagcttta gggattattg gtgcaacagt catgccgcat aacttatatc
     3181 ttcattcagc agtttcacaa actcggaaga tcgatcacca aaacgaagaa gatgtagcac
     3241 aagctgtacg tttttctact tgggattcta atatccagtt gtcaatggct tttctagtca
     3301 atgcactgct attgatcatg ggtgtcgctg tcttcaaaac cggttctgtc caagatcctt
     3361 ccttttttgg tctgtatgaa gctttatctg atccatcagc tatgagcaat ggtattttga
     3421 ttgctgtagc aaaatctggt atcttgtcca ctttatttgc tgtagcactt cttgcttctg
     3481 gccagaactc tactattact ggtacgttga ccggtcaagt catcatggaa ggattcatcc
     3541 atatgcgtat cccgatttgg ctaagaagac tggtcacacg attgctttcc gtgattcctg
     3601 tattgatttg tgttttgttt acaagtacaa aaggaacgat cgaagaacat gccgctttga
     3661 ataacttaat gaatgaatcc caggtgttct tggcttttgc gttgccattt tcgatgattc
     3721 ctttactgat gatgaccaac agccaagcgg agatgggtag ccgatttaaa aattcattca
     3781 tcgtaaaact tcttggctgg ttctctgtga ttagtctaac ttacttgaac ttacgaggat
     3841 taccaggaca gatcgaaagt ttcttcggcg agcaagccac ttccgctcaa ctagcattgg
     3901 ctgatcacat cgcttatgta atcattgttc ttgtccttct cctgttggca tggacgattg
     3961 gtgatttata taaaggaaac aaaaaatatg aacagctttt gcaggaaacg gtagaagaaa
     4021 tcgaataaaa actgattttt ccacacaaag acccttgata gttaggttaa aaatctagct
     4081 accaagggtc tttttattta gaagataaag cttttttcag atttaattta ctgaaaacta
     4141 gcatgcttgt tactacgatg aattgcgtta atggcacgcc tgcaccagtg tagacactta
     4201 aaaaatagtc ctttttttct ggcgcataag aacaaaacca tgagacaccg aaaagaaaaa
     4261 ccaattgtgt cagtattaat ccggtgacca caatcaaaga ggaactataa taggcacgat
     4321 cagcttttgc aaatacggtt cctagtatca taagtaatat ccctgctatt gaagcaatca
     4381 gcacgggaat agccagaact tggtaaattt ttatgatgac attcgcgact tttgctgatt
     4441 ttgttaattg gaaattaggt gttctcggtt cattactatc gatccaattc tccattagcg
     4501 gaaggtttaa taattcttga gcctgtttga cttgttcttg cgttccgaaa ctataatttc
     4561 ctccttgatg gaaatcttta tatcgccatg cataagcgta tcctgattga aggaaatcga
     4621 atacgattgg cagatcactc atcgtctttc catctcctga tcccatcaga tagaactctt
     4681 ttttcttttt gatggttccc ttgtgatagg cttcttctaa ttcttggttg acttttttcc
     4741 aaaaatcatt ggtttctttt gcattgtttt ggtaataacc agattcgctt accacatctc
     4801 gcaatgccca aaagatgata tctccgggta attcttttac ttttccacct tgagtccacg
     4861 cgtgagtcgt atataattcg tcaatacggg tagaaacagt atggaatgta ggagatgcgg
     4921 ctaatgcttt atttagtgca gcatgtgaga cccatatctc actgttttct ttattcaagt
     4981 tcgtttcatc atctattcgg atcagttgtt tcatcaaacg tccaaattct ccactggaac
     5041 ggtcattgat ggttgcaata ccgtatgttg tctcattttt atattcgatc cactgataga
     5101 cgatggaaaa gccaactata ggaagtaaaa acaatgaaag tttcagccat tcccgtttcg
     5161 agagaagaaa gagtcgtttt tttaacttct ttacttcttt gaaagaaaag tcttgcccta
     5221 gcaagatctg gacgaaactg atacccaaag ctactacagt aaaaggaaga agccagatag
     5281 aatcttcgcg aatgaaccaa aagaaaggaa aagcgacaga aagccctaaa aaccatggca
     5341 gcaattttct gctagaaccg tatttgcggt aataaatagc taataaacaa gcgataatga
     5401 tctcaacagc cggaatcacg agggcattac gatatgttcg agtggaatat tcatgtgtaa
     5461 aactaatcgg agaaaacaaa aaataaaaat agatacttaa aagaagatac ttgttcttca
     5521 caatcggttt aaacgcgagt gcaaccaata tactggcaaa aatactgaac atgccgaaca
     5581 aaactgcata agaaagatgc agtttatgtg caagtactaa aaataagctg taggagattc
     5641 ctttggaaag tgttgtagtc gtatactccc ctagccattt accatccaaa atattttgag
     5701 catagtgaat cagcagctga tcatcatagg tcgccgacat atctgttcca tagattgctt
     5761 tactatataa atacaaccga ataattgtaa ataatacggc tatgccaaga ataaagtatg
     5821 tatgtttctt ttttagttgg tcgatcaagg ggtttaaaat gttcagcatc ttttcgtcct
     5881 ttcaatcacg ttttgctact tgtttgtgaa acttttcgtt ttttactttt ctatcagtat
     5941 accctgtttg tctgaattta aaaagagggg gcaaaaggag catggcgaaa acgaatgcaa
     6001 aaagcactcc tttgtttctt aaacaaaaaa ggacaaggca gactgcccaa tccttttttg
     6061 ttgctgtcag gtagaaaact taaagtgtga cttcttctgc taataactca gggaacactt
     6121 cgggatcgaa tgcttgcgct ttgaacatgg aaatttcttt tccatatggt gcgtaagctt
     6181 tctttttatc tttaccgaca tatggggttt ctaatatttt cgaaatgttg tcaaacattg
     6241 gatcatggac gattttgttc aatgtgtcaa atccaattgt tccaaaaccg atattggcat
     6301 gccgatcttt atgagaacct tgtggatttt tagaatcatt gatatgcagt actttaagcc
     6361 gatctaatcc gatgacctta tcaaactctt ccattacacc tgaaaaatct tcttttatat
     6421 tatatccggc atcattcgta tgacatgtat caaaagtaac ggataatttt tcattcaatt
     6481 ttactccatc gatgatttgc gctaattcct caaatgttcg accgatttcc gtacctttcc
     6541 ctgccatcgt ctccaaagcg atctgtgccg tctgttcttt tgtcaaaact tcattcaacc
     6601 cctcaataat tctagcaatc ccagcttctg cccctgctcc tacatgagcg cctgggtgaa
     6661 gggtgatttg agtagcacct aaagcttccg cacggacaat ctcttctctc agaaattgta
     6721 cagcaaatgg aaacatttct ggtttgatcg tatttcccaa attaatgata taaggcgcat
     6781 gaacgacgat atttgttagt ccgttttctg ccataaatgc ttttccggca tcaatattca
     6841 tctcttcgat agcttttctt ctagtatttt gaggagcgcc tgtatagatc ataaaggtcg
     6901 aagatccgta actggctgct tcttctgccg cgcctaacag cattttcttt ccgctcatgc
     6961 tgacatgtga gcctaataac atggatggat tcctccttga aaggcaattg tttcgaacaa
     7021 ttcctgtata ttgataagat gatgtacaaa gaaaatggtt tgtttttctt tgccctgttc
     7081 tccatagtaa ctgaaacttt ccctaaagaa aagcttaaga actctttcat aaaaaagatc
     7141 atttcctcac gttttatctc attcgaaaaa gaaattcctg tcaaaaacag ccttgtctgt
     7201 ttttttgtcc tattatttgc tataattagc gaaataaaac cattagttga ggtgaccctt
     7261 ttgccacgca aaaatactcg aaaaaaacag aatagaagaa aaaaagaaaa atggtttgtt
     7321 cctaagatta tttttagagt gtttcaatca ttgactgttt ttattactgt tcttcttttg
     7381 ttgttcgcag ctctaggtat cggaataggt gctggctact ttgcctatct tgtcgaagac
     7441 acacaattgc cgactaaaaa agaactgcag acagaactag gaaacattac ggaaacgtca
     7501 aagattgttt atgcagataa tacagagata tcaaaaatcc agacagatct gatgcggacg
     7561 actatttcat ctgataagat ttcgccttta ttgaaaaccg cgattatcag tacagaagat
     7621 gaatacttcg ataaacatca aggttatgtc cctaaagctg ttttacgagc actagtttca
     7681 gaagcgactg gcatcggctc gtctggagga tccactttga cacagcaatt agtcaaacaa
     7741 cagatcctaa ccgatgaaac aacattcaaa cgtaaagcaa acgagatcgt tctttctgca
     7801 caagtagaaa aacacttttc aaaaaatgag atcattacta cgtatttgaa tgtctcacca
     7861 tttggtcgga ataataaggg gcaaaatatt gctggagtcc aggaagctgc tagaggaatc
     7921 tttggtgtag atgcaaagga tgtgactctg ccacaagctg cttatatcgc tggtttgcct
     7981 caaagcccta tcacgtatag tccatacacg aatacaggcg cgctaaaaga agatctttct
     8041 gccggtcttg ccagaaaaga ttttgtttta ttcagtatgt accgtgaagg acaaattacg
     8101 aaagaacaat acgaagaagc gaaagcctat gatcttacaa aagactttct cccacaacaa
     8161 atcgcggaac agactgacag agaattcttg tattataccg tgatgaacga agcaacaagg
     8221 attattgcac aacaattggc agaaaaagat aatgctgata tgtcagattc atcggtcagt
     8281 gaagcttatt atcaaaaagc acagcaaacg atccaaaaca aaggatatac gatccattcg
     8341 acgatcgata aagatatcta tgctgcatta caaaccggcg tagaaaatta tggctacatg
     8401 ttagatgatg gtgctggtag ccaagtggag actggaaatg tattgatgga taacagaacc
     8461 ggacgtatct atggcttcgt gggtggtcgg aattacagcc agaatcaaaa taaccacgcc
     8521 tttgatacag aacgtcaagc tggttcatcc atcaaaccgg ttcttgttta cggtcctgca
     8581 atcgatatgg gacttgtcgg atcagaatcc cgtgtatcag attatgcaac gacttggcaa
     8641 gaaggaaaaa atgctggtga gaaaatcgtc aatgcgacca atgaagggtc gaacaccttc
     8701 caaaccgtcc gtgagtcact ggaatggtcc aacaatatcc cagcttatca cttatatcaa
     8761 gatgtgttga attccggtgg atcgaaacaa tacgcttacg agcactactt ggcgaagatg
     8821 aactacccag caaatgataa ttggggcgtt gaatcggctc ctttagggac agtagatgta
     8881 acgacgcttc aacagacaaa tggattccag gccttagcaa atggtggagt ctacgaagaa
     8941 ggctatatca ttgattccat cacagataat gcaggtaatg tgatttataa acatgaaagt
     9001 aattctgtcc gtatttattc cgaagcaacg gcatcgatca tgaatgacat gatgcgtagt
     9061 gtgatcaatg aaaaaatcac tacaccattc aaagatgtca tctcaagctt aaacggaaat
     9121 ctaggaaatg cagattgggt cggaaaaact ggttctacca atgaataccg agattcatgg
     9181 ctgatcgtct caactccatc tatcactatc agtagttggg caggacacga tgacaatact
     9241 ggaatggaca gccaagcaag aatacgttcg gctaattatc ttgccaatct aatcaatcaa
     9301 gcttaccaag caaaccctga gattttcggc actgatgaga aatttgactt atcatccgat
     9361 gtgatgaaga aaaaagtatc tgggttcaca ggacaaacag ctggaaaagt tactgtcaat
     9421 aaaaaatcca tccaaacgcc aggaaaaaca gtggagtcac tttgggcaaa aaatggtccg
     9481 aagaaaagcg cctttaaatt cggcgtaggc ggtactgatg aaaactatac agattactgg
     9541 aacaccgctt cagcttatgc tagagcgaat ccagagacca aagaatcaga cgataaagat
     9601 gattgatcta atagattaat cagaattaaa gggagctgcg acaaaaatct tgtcacagct
     9661 ccctttttcc gaataaatgg tgttcaaaag cttacatcta cgatattaag acaaatttat
     9721 gtaaaaatgt gaaaaac
//
LOCUS       DACEXQ010000091         9604 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.091, whole genome shotgun sequence.
ACCESSION   DACEXQ010000091 DACEXQ010000000
VERSION     DACEXQ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.091"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..1487
                     /locus_tag="IXH04_001555"
     CDS             <1..1487
                     /locus_tag="IXH04_001555"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF014820.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAQ1852164.1"
                     /translation="VVSQELQLDEEKVNIYGGAIALGHPIGASGARILTTLAYGLLRE
                     QKRYGIASLCIGGGLGLAVLLEANMEQTHKDVQKKKFYQLTPSERRSQLIEKNVLTQE
                     TALIFQEQTLSEELSDHMIENQVSEVEIPMGIAQNFQINGKKKWIPMATEEPSVIAAA
                     SNGAKICGNICAETPQRLMRGQIVLSGKSEYQAVINAVNHRKEELILCANESYPSIVK
                     RGGGVQDISTREFMGSFHAYLSIDFLVDVKDAMGANMINSILESVANKLREWFPEEEI
                     LFSILSNFATESLASACCEIPFERLGRNKEIGEQIAKKIQQAGEYAKLDPYRAATHNK
                     GIMNGIEAVVAATGNDTRAVSASIHAYAARNGLYQGLTDWQIKGDKLVGKLTVPLAVA
                     TVGGASNILPKAKASLAMLDIDSAKELAQVIAAVGLAQNLAALRALVTEGIQKGHMGL
                     QARSLAISIGAIGEEIEQVAKKLREAEKMNQQTAIQILEKIREK"
     gene            complement(1817..2521)
                     /locus_tag="IXH04_001556"
     CDS             complement(1817..2521)
                     /locus_tag="IXH04_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852165.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(2708..2953)
                     /locus_tag="IXH04_001557"
     CDS             complement(2708..2953)
                     /locus_tag="IXH04_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852166.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(2970..3821)
                     /locus_tag="IXH04_001558"
     CDS             complement(2970..3821)
                     /locus_tag="IXH04_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ1852167.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            3987..4991
                     /locus_tag="IXH04_001559"
     CDS             3987..4991
                     /locus_tag="IXH04_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAQ1852168.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(5232..5588)
                     /locus_tag="IXH04_001560"
     CDS             complement(5232..5588)
                     /locus_tag="IXH04_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ1852169.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            5956..7143
                     /gene="tuf"
                     /locus_tag="IXH04_001561"
     CDS             5956..7143
                     /gene="tuf"
                     /locus_tag="IXH04_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ1852170.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(7331..>9604)
                     /gene="mprF"
                     /locus_tag="IXH04_001562"
     CDS             complement(7331..>9604)
                     /gene="mprF"
                     /locus_tag="IXH04_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ1852171.1"
                     /translation="GGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLS
                     FLLVVFTPVAPFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSL
                     LEWTGVLFSFLSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLS
                     AIGVPREIVVVWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHK
                     IVVVLLYFSGIMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRG
                     IAARVKRAYLPTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYS
                     WEWRTIDGIIIGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAV
                     SFMIVLFVHFLQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGE
                     PTVLLQFACFNNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMIL
                     HEFGYDFIKMGEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMS
                     TFKNISDNWLGSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKV
                     GTIDLMRYDSKKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQER
                     IAYLVYEFGSHFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVE
                     QTQGRISGLKKWIRRKY"
BASE COUNT         3108 a         1763 c         1907 g         2826 t
ORIGIN      
        1 tcgtagttag ccaagaactg cagttagatg aagaaaaggt caatatatat ggaggagcaa
       61 tcgcattagg ccatccaatt ggtgcaagcg gtgcacgtat cttgactaca ttagcttacg
      121 gattgcttcg tgaacagaag agatacggta ttgcttcatt atgtattggt ggaggtttag
      181 gcttggcagt cttgttagaa gcgaatatgg agcaaacaca taaggatgtg caaaaaaaaa
      241 agttttatca gcttacgcca tctgaacggc gcagtcaatt aatagaaaaa aatgtattga
      301 cacaagaaac tgcgttgatt tttcaggaac aaacactttc tgaagagctc tccgatcaca
      361 tgatcgaaaa ccaagtgagt gaagtggaaa tcccaatggg gatcgcgcag aattttcaaa
      421 tcaatggcaa gaaaaaatgg attccgatgg caacagagga accatctgtc attgcagctg
      481 caagtaatgg tgcaaaaatc tgtggaaata tctgcgcaga aacacctcag cggctgatgc
      541 gagggcaaat cgttctatca ggaaaatcgg agtatcaagc agtgatcaat gcagttaatc
      601 atcgaaaaga agagctgatt ctttgtgcaa atgaaagtta tccttcaatc gtgaaacgcg
      661 ggggcggtgt tcaagatatc tctactcggg aattcatggg gagtttccat gcatacttat
      721 ctattgattt tttagtggat gtaaaagatg caatgggcgc taacatgatc aactcgattc
      781 ttgagagtgt ggcaaacaag ttacgggagt ggttccctga agaagagatt ctattcagta
      841 ttttaagtaa ttttgctaca gaatcattgg cttctgcctg ttgtgaaatc ccattcgaac
      901 gcttagggag aaacaaagaa atcggtgaac aaattgccaa aaaaatccaa caggcagggg
      961 aatatgcgaa gcttgaccct taccgtgcag caacgcacaa caaagggatc atgaatggta
     1021 tagaagctgt agtggcagct acaggaaatg atacacgtgc ggttagcgct tcgatccacg
     1081 cctacgcggc aagaaatgga ttataccaag gattaacaga ctggcagatc aaaggggata
     1141 aactagttgg aaaactaacg gtgcctttgg ctgttgccac agtaggcgga gcttccaata
     1201 tcctgccaaa agcaaaagca tctttagcta tgttagatat tgattcagcc aaggaactag
     1261 cacaagtaat tgctgcagta gggttagctc aaaacctggc tgctttgcgt gcattagtta
     1321 cagaaggaat ccaaaaaggc cacatgggac ttcaagctcg ttcattagcg atctcaattg
     1381 gggctattgg tgaggaaatc gagcaagttg cgaaaaaatt acgtgaagca gaaaagatga
     1441 accagcagac agctatacaa atattggaaa aaatccggga aaaataaaaa agcaatatct
     1501 ttagaagaat aaagcaagat ttacagttat tttatttact gcagatcttg cttttttctc
     1561 tatcagaaaa tatgtttatt attagaattt tttatagtta aggttaggca tcttaagaaa
     1621 tcaggcaact ccaagatatc ttcaggtaga agagaagtcg cctaacattg taggatcata
     1681 ggtgatttgt tggacaggtc aagctgatat ttgagcctga tgttagaagg aatttctttt
     1741 tacttgctgt agaaattatt atatgactaa aaaggctggg attttaaatc ccagcctttt
     1801 atgacaaata gaatttttat cgatcagtaa tcgctgtcgc tgcaaattgt tcgatcaaag
     1861 gaatcaaagt caatttgact ttgtatttcc gttcgagtgc tttttcgata acgaactcat
     1921 tttcagtagt catagaaata gttgctaatt tacgaggttc atcagcattt ttttcattaa
     1981 taactacttc atgttcattt tgttctgtgt aaaatttagg atactcagca acggcttttg
     2041 aaaaatcagc aaaacttaaa gcggcattcg tatcagttga gcgtgtattt tcagttgtgt
     2101 ccgcttttgg tttttccgtt ttttcttctg gcacactgac tgtttcttct gtaactggtg
     2161 tcgcaacagc ttgcgcagtt ggttcatcgt ttttcaatcg ttgatcattc cagtaagaca
     2221 cgatttcaat aagaagattg tcacggaatc gttcgaaaat cccgttttct cctgaacgaa
     2281 ttctctttaa tacagtattg aagttgtcat cgattggcac gttgatccgt gtatcttcat
     2341 ttgttttccc agctaatccg atcgttaaga tttgacggtt tttgggatca agcatgactt
     2401 gtaacggcaa accataacgt cgcaagctct cttcttctag agaacggaat aattgtcctc
     2461 ttacaccatc caatgaagga cggaattgat ttaataattt ttgtttctgt tttttcttca
     2521 tatgttctct ccttcgctca taccctttcg attataagaa aaaaacagcc aaatgaaaag
     2581 gagtaatgca aaaaaacttg cgtatacgca aattacaaca gaaaaatttg cgtattttcc
     2641 cgttttttga aagaaaaata aaaaatggaa cggattatgt atatatacat aatccgttcc
     2701 attctttcta ttttttcggt atcaaggcaa aaacaggtag ttcaggtgaa taaatgactt
     2761 catgttcgtg ccagggtttg accattagcg aatgatcgaa ccgttttttc agttcttcga
     2821 tgatttgatc atgtgtccag tttcttgcag gaaaatgttg gattttctct gcatcgatca
     2881 aaaagacaaa atcggaatga aaagtctggg taacttcttc cgagagagaa ttcaagcgtt
     2941 cgattggatc cataaaatca cctttcgact taaaattggg tagtatctgg gtcttttgct
     3001 ttgccgacta caccatctaa ctgatcgatt tgtttcattt cttcttctgt taattcaaaa
     3061 tcaaataatt cagtattttc tttgattctg cttggtgtca ctgacttagg aagaggaagg
     3121 aattcgtgtt gcagcgacca acgcaaagca acttgtgcaa tcgtcttgtc atgtgcttca
     3181 gcgatttgtt tcatttcagg cacatcgaag attttaccag tgcctaatgg actatatgct
     3241 tcgaggatca tatcatgttt ttttgcatac tcaactacag ttgtctgtaa ttcgcctggt
     3301 gcaaggaaaa cttgattgac catcggcata atttttgctg tttttgctag tgtatctaga
     3361 tggtggggta agaaattgct cacaccgatt gctttgattt tacctgcttc atacagttct
     3421 tcaaatgctc gccatgtatc tgcatttgct tgttcccaat tatcacgaaa agcaacaggg
     3481 tttggccagt ggatcaaaaa cagatctaaa taatctgttt ggagcttctt caatgattct
     3541 tcaaaagaag acatcacaag ttcatagctg tggtttgcat tccataattt tgttgtcaag
     3601 aatatctctt ctctaggaat tccactttcc ttaatcgctt ggccgacgct ttcttcattc
     3661 ttgtatcctt gggcagtatc gatatggcga taacctgcag ccagcgcttc tttaacagag
     3721 gaaacagcga catccccatc aggtgtttgc catgtgccga atcctactac cgggatttcg
     3781 taaccatttg atagacgata agtgcttgtt aatgaattca tactttcagc tcctttttta
     3841 ttcgttcaac tataagtgta gcgcattttt cgaaaaaaag aatccatttt gtttctactc
     3901 ttgaaatttt tgtttaaaag aaaatgattt tgattctggt atttttgatt ttttttccgt
     3961 aaactaagac agaagggaat gagaaaatgg atatctattt aaaaaaagca attctgcata
     4021 ttattgacag agaaactggt tctccagtct tttcacaaaa agagctggat ctgacaaaag
     4081 aatatattcg tgattttctt caaaagaaga tccaaaaaat ctcttctgct caaacaaaaa
     4141 ctggacaact agcagaagaa agcatgatcg ttgaatcact taaaatcgct caagaaaatt
     4201 ttgtggaagc aagtgaagca atcgttcagc ggtggttcga tatctaccaa gaaagtgaag
     4261 aagcacctag tgcagatgta tttgttgtct tgtatgaatt agatacagtg atgtacttat
     4321 ctttattgaa agtcaattat cgtgaggctt acactcactt tgttgaagca gacgaagcag
     4381 ggatcgacaa ccgcttgatt ttgaatcgag caattctagg ggcgaaaaca caaaaagcag
     4441 atgaagcatt cgctgtgaat ttgactgatt ggacctacga attaattgaa aaaaaatatg
     4501 agttttctgg caaaaaagag ctttattttt ctggaagagt gatcgaaagt gttcctgcac
     4561 cttctttaga agaaaatgta aaagtgatcc aaaaagtagc caagcatctt ggaaaaaaat
     4621 ttgagacaga agaatttgat atcatggctg atttgaaaga agctgtctat gatacgatag
     4681 aagaaaaagg tcggttggat catgagatga ttgcggaaaa agtcttcaag gaaaacatca
     4741 cggcaaaaat ggcttttaaa gaagaagtac aagagcaagg atttgtccca gaagcacctc
     4801 ctgtaaaaga agtacaagaa atttctgaga aaaaatttgg aaaacaaaaa ttcaagctat
     4861 caaacgggat cgaactgatc gtaccagtag atgtatatcg caacccagat ttgattgaat
     4921 ttgtgaataa tccggatggt acgatatccg tgatgatcaa aaatgtggat gaagtattga
     4981 atcgtttata aaaatgacta gaatattagg tgttcaagta ccagtgatca gttaaatgaa
     5041 ttgtgtcaag agaaggatcg gaaaagacga atataacaaa aaaagagcgt gagacaaaag
     5101 taaagaacac ttttgtctca cgctctaaac acgtataaac ggcggaagca gaagcaactc
     5161 cttcttattt cttggagtta aacgcttctg tcccagcctc tttttatcta ataaaatttt
     5221 aagaccattt actaattatc gattttttct gccgcatttt taggcacttc agaagccttt
     5281 atttcttccc agcttaatac acctttacga ggattgactt tgagtttaag atacgcattc
     5341 atctttaatg gatgttctct ttcttcgcga agcgtcacct gttttttttc accatcttta
     5401 ttataagccg cttgcttgta ggtataagtc gtgaatttct ctccgttatc tgcttgggta
     5461 gctgacattt cgccattcgt cgtgattttt gtataataag cttcgccgcc gtaaaaataa
     5521 tgataagctg caaaacttcc gccgagaaat atgcctaaaa cgacaagtac gccaattaat
     5581 tttttcatgt acttcacctc tttccttatt attaacgaaa tagaagattt cgtctattga
     5641 aagttgttgg caacagctga attttaaact tttcctcatt ttgttcttat tgcttcaaaa
     5701 gaaagttaag gactatcttg aagtttttct caaaaaacac atttttttat ttataaaagg
     5761 tacttttttc gcgtataaga cagacgagtg ctcttagttg actttgacca atattgacgt
     5821 ataataagct tagttcgaat cttacaaaaa gtaagtcgga cgtattcttt ttgatttagt
     5881 caaaaaaaga ctataataaa aaggtgagag ctgaaatttt tcagtgatct atattattta
     5941 ggaggtttgt aaattatggc aaaagaacat tatgacagaa gtaaaccaca cgttaacatt
     6001 ggtacgattg gacacgttga ccatgggaaa actactttaa ctgctgcgat cactacagta
     6061 ttaggcaaaa aaggcctagc aaatcctcaa gactatgcta gtattgatgc agctccagaa
     6121 gaacgtgaac gtgggattac gatcaatacc gcacacgtag aatatgaaac agaaaaacgc
     6181 cactacgcgc atattgatgc accaggacac gcggactatg tgaaaaacat gattacaggg
     6241 gcagcacaaa tggatggtgc aatcttagtt gtttctgcaa ctgacggtcc gatgcctcaa
     6301 acacgtgaac acattttatt gtcacgccaa gttggtgtaa aatacctgat tgttttcttg
     6361 aacaaagttg atttagtcga tgatgaagaa ttgatcgatt tggtagaaat ggaagttcgc
     6421 gagttattga gcgaatatgg tttcccaggc gatgacactc ctgtgatcaa aggttccgca
     6481 ttaaaagcat tgcaaggcga tccagatgct gaagctgcta ttatggaatt gatggataca
     6541 gtagatgaat atatcccaac accagaacgt gatacagata aaccattact attgccagtg
     6601 gaagacgtct tctcaattac aggtcgagga actgttgcct caggtcgtat tgatcgtggt
     6661 gctgttcgtg tcggtgatga ggtagagatc gtagggatca aacctgaaac acaaaaagca
     6721 gttgtaacag gtgtagaaat gttccgtaaa acgttagatt acggggaagc tggggataac
     6781 gtaggcgtgt tgttacgggg gatccaacgt gacgatatcg aacgtggaca agtacttgct
     6841 aaaccaggtt ccattactcc acatacaaaa ttcaaagcag aagtgtacgt gttgacaaaa
     6901 gaagaaggtg gacgtcatac tccattcttc aacaactatc gtccacagtt ctacttccgc
     6961 acaactgatg ttacaggaac aatcacattg ccagaagata cagaaatggt catgcctggt
     7021 gacaacgtga cgattgatgt tgacttgatc catccaatcg ctgttgaaaa cggaacgaca
     7081 ttctcaattc gtgaaggcgg acggacagta ggttcaggta tcgttacaga gatcgaagca
     7141 taagaaacgc gactcgaata ttttggtaat gaaaaatgtt taaaataaaa gagcggtcca
     7201 aacaggattt ctgtttggac cgctctttgt tcttcaattg tataagaaga gattgtagaa
     7261 gcatgaactt tcgttttact tcagtctctt ttattcatca tgagagtgtt catctatcgg
     7321 tatgcagttt ttaatacttt cttcgtatcc attttttcaa accacttatt ctaccttgtg
     7381 tctgctcaac tggcgcatta tcaataatca gcaaagcaat cataacatat gcaatccaac
     7441 tatctctaga atatagagta taacgtgaga cccagtttgt agcgtacttg cttttatatt
     7501 ctcttaatcc atggaaagaa taaaaatgag aaccaaactc gtaaaccaag tatgcaatcc
     7561 tttcctgtaa aaaactcttt ctggaagttc cgacatttga aagaggggcc atccctaaat
     7621 tgaaccattg gatatgttct tctttcatat aggcaaacaa atgcaagaac aagaaatcca
     7681 tactgccaga aggtgctttt ttagaatcat atcgcatcag atcaatcgtt cctactttat
     7741 tgttcgtgta agtaggcatg atcgttgcaa aagcgacaat ttcttcttgg gtatttttga
     7801 caactgcaat aggcgctctt tgtagataat cttcagagaa aaagcctaaa gaaaaccctt
     7861 tttcctttcg tgaacccaac caattatcag agatattttt gaaagtactc atttgctcag
     7921 tagaaaatgg tggttgtaag acatcaaatg taaatccttc tcgttcgatc cgattcaaga
     7981 cagcacgagt gcccttcatt tttttccctg aagttgtaaa actattcagg tctacataag
     8041 cttcttcccc catcttaatg aaatcgtagc cgaattcatg caaaatcata acaatctctt
     8101 cgcttgtttc gtagaaaact ggtaaatagc atagtcgatc tgtttcttcg ataaaagctt
     8161 cgattgcttc tggaaaatct tctttcttac cagaaggatc acccattaca atacatttat
     8221 tattgaagca agcgaattgg agaagaacag ttggttcacc gtctttttca taggcaaaca
     8281 tccgcttatc ttttaagaag atcaattgac tatcggagtt tcctccataa gttgtaagga
     8341 tcttcagggc cttttcttca ttaaaggctt cgccaatctg tttcttttct ccttgaagga
     8401 aatgaacaaa tagaacgatc atgaaactaa cagcgatgat ggccaacaat cccgaaaacc
     8461 aaatcttttc agaaggaaat aagaaaaagg aaataaaatg gtgacgccga tgagggaaat
     8521 ccggcagatt gtatacccca ataacgatgt aaagcaagga cagtgcacca ataatgattc
     8581 catcgatcgt acgccactcc catgaataga tcaattgttc ccgaaataat tcgtttttag
     8641 aagcaagaat gatcagaagc agaatagaca agaaaaccac cggtgtgaaa ctaaaatcac
     8701 ttaataaaac ataaagtaaa gctagaacaa tcaagaagat tgtaggcaaa tatgctcgtt
     8761 tcacacgtgc tgcgatccct ctgcccataa tgattaaaag aaatcccaat agtatactag
     8821 gaaattggat aataaaatga aatttcaatg ggttcaaact atgaagccag cgaaactctg
     8881 taaaagcttg cgggatagtg gcagaaagta ctagcatgat tccagaaaag tataataaga
     8941 caaccacgat tttatgcgca atttctgttg ctaattgttt agggatacca gaataacgct
     9001 gatcaaaagt ggaaccgata tttttgaaga aaaagacgat tccaatcaaa aacggaatga
     9061 tgtagtagaa caggcgatag agcaagatcc acacaacgac tatttctctt ggcacaccaa
     9121 tagcggacaa cccgataatc atcattacat cgaaactccc aatttctcct ggaatcatag
     9181 acacgattcc tatcactgaa gcagcaatga ataatggaat tgtttgccac agatcaatcc
     9241 ggatttccat taaataacca acagaaagga aactaaacag gacaccggtc cattccaaca
     9301 atgaaactag caatagctgt ccttgagttt taagtgaggt accaccaagg aatcctttct
     9361 ttttgaaagt agtaaataaa aagacgacag gaaaatatag accgccgccg atcaaccata
     9421 tccaatattg cttcaaaaaa ggagcaactg gcgtgaagac tacaagtaaa aaagaaagca
     9481 gactgtaaat agaaagtcca gccatcaaaa acaagaaaac tttcgataat gcttggatca
     9541 cgtttctgct ctctttcttt tgtccatata gttcagagcg aagccccaca ctaacaaacc
     9601 cgcc
//
LOCUS       DACEXQ010000092         9500 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.092, whole genome shotgun sequence.
ACCESSION   DACEXQ010000092 DACEXQ010000000
VERSION     DACEXQ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.092"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            806..1006
                     /locus_tag="IXH04_001563"
                     /pseudo
     CDS             806..1006
                     /locus_tag="IXH04_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1493..1672
                     /locus_tag="IXH04_001564"
     CDS             1493..1672
                     /locus_tag="IXH04_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852172.1"
                     /translation="MTELTADMSDTWLQEYLFPEKQTTARGYLPVDWEYVHKELMKKN
                     MTLKLLHREYGVHGT"
     gene            complement(1718..2323)
                     /locus_tag="IXH04_001565"
     CDS             complement(1718..2323)
                     /locus_tag="IXH04_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1852173.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            2505..2840
                     /locus_tag="IXH04_001566"
                     /pseudo
     CDS             2505..2840
                     /locus_tag="IXH04_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3005..3232
                     /locus_tag="IXH04_001567"
                     /pseudo
     CDS             3005..3232
                     /locus_tag="IXH04_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            3358..4329
                     /locus_tag="IXH04_001568"
     CDS             3358..4329
                     /locus_tag="IXH04_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1852174.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICKENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            4469..5782
                     /locus_tag="IXH04_001569"
     CDS             4469..5782
                     /locus_tag="IXH04_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ1852175.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQISGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            5825..6691
                     /locus_tag="IXH04_001570"
     CDS             5825..6691
                     /locus_tag="IXH04_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1852176.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLMPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            6688..7536
                     /locus_tag="IXH04_001571"
     CDS             6688..7536
                     /locus_tag="IXH04_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006639650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1852177.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            7550..9022
                     /locus_tag="IXH04_001572"
     CDS             7550..9022
                     /locus_tag="IXH04_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAQ1852178.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            9039..>9500
                     /locus_tag="IXH04_001573"
     CDS             9039..>9500
                     /locus_tag="IXH04_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1852179.1"
                     /translation="MIYGSIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEM"
BASE COUNT         3008 a         1598 c         1958 g         2936 t
ORIGIN      
        1 tctatatttt gatatacaaa ttacagcttc agatactaag ttgcataaac cttttccgga
       61 ccccatacaa aaggcaattg atgaattggg cataaaaaat aagaaacgat ttatattggt
      121 gattcattat atgacatgca aagtgcgaaa gcttgtggga tagcatttgg tttagctaaa
      181 tggggagcca aaaatataga aaattttcaa agtgtagata taattattga ttctcctcaa
      241 gaaatacttg atttgttaga aaaatagaca aagagcgtga gataaggata gggtctcacg
      301 ctctttgttt ttcgccattt aaagattgaa atttttgcca tttaaaaaga aaagtgacag
      361 ataggaataa aaacttatta aatgtagtta tgatctgtta tatttcattt ctaatactag
      421 gtcttccaca agcagtgttt cctgaaatgt tttctggaga ctggggtatt gtcagctatt
      481 ctatattaac attgattgct atggttttag gtacttcatt ttataatgct tttgtaagta
      541 agcatgttga ggaatatgaa gtattaatgt acttaaaaga gaaaaaaaca agtaattaat
      601 atgagaacac cacgcatagt ttttattttg agatattcta gaatgaaaca cattaagaac
      661 gaaagcaaag caaaagacgc cagaacttct actaccttct aagttttttc atatttgttt
      721 gttatgctat acatagatct gcataattag acttgcgctg cccgttattg aagtttcagg
      781 cagcgctctt tttgggggat cgataatgga agaagaaaaa atatttgaga aacgctggca
      841 gctagcatca agtgaacaaa gagcaagata caataatttg atcacctcct accccactgt
      901 tgactagaca tataaggaaa agaaatactt actttggctg tgccagttag atatagatac
      961 ctttgaaaca tttgaagtga tcctagataa aattaaacag aattgatcag aaacaagcga
     1021 agccttctct cggttcgctt gttttgctat ttattagttg gctcagggca actctaagct
     1081 cgtttgaaag aaaaccgatg gataatcccc ttgtttgtac cactttagaa tacatggatg
     1141 tacgtattgc cattgactcc tattagttac tgaaaaaaaa tttaaacaag ttgagtattg
     1201 cagataaata gctccagtga ggtgaataac tgctccactc aaggcaatag atagctccat
     1261 ccagggcaac aaactgctcc actttcatat ttaatagatg aataaccttc tgtataatag
     1321 aaaggcatac ctttcggtat gacaacttat acagaatgtt attttattgg ttcactatcg
     1381 aaaaattttg gagctttatt tttccggtat ttctcaacga acgattagtt taactgtggg
     1441 aagctccaga catacgattc gagaagttac ttctcgagca gacaggaagg gggtgactga
     1501 actaaccgct gatatgagcg atacttggct gcaggaatat ctttttccgg aaaaacaaac
     1561 aacagcacga ggttaccttc ctgtagactg ggaatatgtt cataaagaac taatgaaaaa
     1621 gaatatgaca ttgaaactcc ttcatagaga atatggggta catggaacat agaaacgaaa
     1681 atgcacggta gaaaagcgaa tgaatatagt ataaattcta caaatcattc gctttcttta
     1741 ttttatatat ggttggtcga gagagaccat tttctttggc aatttctgca actggaactc
     1801 cttgatttaa catgtccacc acagttttaa agactaatcg tttttgcgga tctgttgagt
     1861 tctcagaata ttcaattggt cgccccttat atcgaccagc ttttttggcc aataatactc
     1921 cttgccgctg acgttcacgg attttctcac gttcgttttg tgcaatataa cttagcaatg
     1981 ataaaaacat atcgaacata gcagtatcta ggagttcatt tccagtatta aaatctaaaa
     2041 actgtgcatc tagtattttt aatgctactt ttttctgttt cataaaggta actgtatttt
     2101 ttatgtcttc ataatctctg cctaggcgat ccagtgatgc aacaatgaca caatcacctt
     2161 ctcggatata atttagtaat ttttgaaact caggtcgctg gatatcttta ccactggctt
     2221 tttcttggaa taatttatca atgttaaggt cttccatcat tgccaattgt cgatctaaat
     2281 tttgatcttg tgaagacact ctaatatatc caatttttgc cataaaaact ccttttaaaa
     2341 aatgatataa tttacatgta aaatttactt gtagttttac tgttatcata gcatttttaa
     2401 aaataaattt taagtctact tataatttac tttggaggtt ttgaatgaat gaaattaagc
     2461 gcaataaaaa ttcttttggt gtttatttag gagagcctag ctcggaacag ttaaataaag
     2521 gtgaagctcg tcatgctcta tgtcgaaata tattttatgg tagaaaaggc aaactttatc
     2581 aaacttatta cgacggtatg gaggaacagc tcaacgcatt aggattagta actaatgcta
     2641 tcatatattg gaattcacta tatttagaaa aagtctttga acaaattaaa acggaagggt
     2701 tcgactgttc cgaagaagtg ttacgcaagc aatttccatt aattagtgat cacattaatt
     2761 ttgttggcaa atatagtttc cgctatgaaa aagaattatc gaatggaata ctacgtccgt
     2821 taaatataga gaattattga gaatgttcgt cttttggttc ctaccgtgca ttttcgcacg
     2881 gatgttccag atactatttt tgaatgtata aaattgaaaa cacaagaata ctaataaatc
     2941 aacgattctt gtgtttttaa tttttcacta ccgtgcattt tcgtttctat gttccatgta
     3001 ccccaatctt gaaaaattaa ttaaagactt agataaagcc attgacgact tagtcgtggt
     3061 gctaccggag tatcaatgtt taaccagtat cccaggtatt ggtcctgttt aggctgctgg
     3121 aatactcgct gaaattggtc caattgaacg tttttctgat gaaataaaat tgacaaagta
     3181 tgccggccta tattggcgac agagtcaatc cggtaaatct gaatacgaaa atttttttac
     3241 gaaaagcttc acgtctaata tctattttaa aataaaaata ttcctgctaa tttggaatcg
     3301 tttccttctt ttggtataat aatacctgaa attcttttga gaggagttgt tcgtgaagtg
     3361 aaaactacta tgaaggatgt tgctgcgtta gcaggagttg gtgtgggtac ggtatctaga
     3421 gtaataaatg gagtaaaggt gaaagaggtt accaaagaaa aagtaatggc tgcaattagt
     3481 gagcttaatt atgagcctga tgaatatgcc agaggtatga aaacgaatag tagcaataca
     3541 atagctctta ttttacctac gatttggcat cccttctttt ccgagtttgc ctactatgta
     3601 gaggaagctc tcagccaaaa aaattataag ctttttttgt gtaatgcgaa cggcagtgcg
     3661 gaaaaagaaa aagaatatat ccaaatggtc aagcagagta aagtagacgg aattataggt
     3721 attacctatt cagacattga taaatatgta tcatcaaatt taccatttgt cagtatagac
     3781 cgccattttt cggaacaggt ttcttatgta acggcagata attatttagg tgggcaaatt
     3841 gccgctcaag aactacttac tcgtaactgt aaaaacttgt gctatgttgg gggtgtttca
     3901 ccttacccta acgaaacaaa caatcgtaaa gctggttttt acaatatttg taaggaaaat
     3961 aaaatagctg tgaaggtatt ggatatgcct gaaccaatta gcaatttaga ggaacaattg
     4021 ctcgcttttt tttcggataa tcctgaaatt gatagcattt ttaccatcaa tgatttcatg
     4081 gctttggatg tgataaaagc ccaaaaaaaa ctagggaagg aagcacctaa ggattttcaa
     4141 attattggtt ttgatggagt caagctatcg ttgcaaaaag actatttact atcaacgatt
     4201 gtacagccga tagaggaaat ggccaaggtt tcagtagaaa cactcatggc gatgatttta
     4261 aatgaagaac cgcctaagcg tgtggtagta ccggttttct ttcacgctgg agagacgact
     4321 aaagagtaaa aaaccggttt ataacgattt ttttgagata ggttgacaga aagtgatttc
     4381 aatgatatct taaaaccaat taattggaaa cgttaccaat taattgaaaa ataaaacgga
     4441 aacgtttcaa aaaataggag gaacatcgat gaaaaagaga atgttagcaa ttgcagcaat
     4501 tttagcatta acagctggag gacttgcagg atgtggcagt aaggagaaaa gttcaggtag
     4561 cagtgatcca ggagaaatta agatttgggt tcagttttct gatgaaacgc ctgaaggaaa
     4621 agcttgggca gagattgtga acaattttaa tgatgaatac gagggtgaat ataaagctgt
     4681 tactgagtat attcctcgta gcggaagcgg cggtggatat gaggataaag taaatgctgc
     4741 aattacaacc aatacccttc ctgatgttat tacgttagat ggaccgaata ctgcagctta
     4801 tgctcattca ggagtcattg ctccattaga tgattatttg aaagatgcgg acatggatga
     4861 tgtattagac agcattattc agcaaggaac ctacgatggc aaaatgtatg cgtttggttt
     4921 ctcagagtcc agtgtgggtg tctattacaa taagtcgctg ttcaaagatg ctggaattac
     4981 agaagatcaa ttgccgacgt tagaagatcc ttggacttgg acggaatttc aagcaatttg
     5041 ccaaaagctg actgataaat acaaaacgcc tgcaattgat atgcaaatgg cgtcgggaga
     5101 cgaaatgctg acctacgcat atacaccatt tatatggtcg aatggcggcg atgtcgttaa
     5161 tgctgatgga accaaagccg aagactattt taacggtaaa aaatccgttg aagcgttgga
     5221 atttattcaa gaattggttg aaaaaggtta caccacaatc agtcctattg aaaaaggatt
     5281 tgaaactggc aaatatgcca tgatgctgag cggttcttgg acaatggcag atttagagga
     5341 aaactatcaa gagattgatt atggtatttt gccttacccc gtttcagatg caactgggga
     5401 gttagtttca ccaaccggaa gctggcagtt agcaatgact gagaaaacag agaaaaagga
     5461 tgcttctgcg gcctttatta agtttgcaac cagtacagaa tctagtcgta ttatgagttt
     5521 agggaacagt gttctaccta tcagaaattc aacagttgat ttaattaaag atgaagtgtc
     5581 ggaaggaatg caggtattaa ttaagcaaaa ccaaatttct ggacatgccc gcccagtggt
     5641 tgtagcttat ccgcaagtat ctagagcatt ccaacaagca gttcaagata ttagctactt
     5701 cgaggaaaac ccagatgtac aaaaagtagc agatagtcgc gctgctgaaa tgcaacaggc
     5761 aattgatact tcactaaaat agaagtttct aaagggggcc aatcaagtgt taaaaaaacc
     5821 gacaatgaaa gacaatttga taggttacgg ctttctagcg ccagctttgc tgctgctgac
     5881 gatttttctg atcatacccg taatcatggt aatttattat gcctttactg attattattt
     5941 gttaatgcca gatgctagac aatttatcgg cttagaaaat tttaagcgac tgttcgctga
     6001 tccaattttt gtaaaaagta tttggaacac ggctaaattt gttgtttgga ttattccggt
     6061 ccaaataggg gcagctttag gaatggcact tattatcaac aaaaatcgta aaggaaatat
     6121 cttttttaaa gtagcctttt ttgcgccagt cgtgatgtcc ttagtcgtta tttcgatttt
     6181 atggttgtac ttgttaaatc ctaatgaagg tctggttaat acgctatttg caaagattgg
     6241 aattgcttcg cagccttttt tgactagtcc caaacaagca atgtatacgc ttgtgttcgt
     6301 atcggcatgg caaggtgcag gatatcagat gctgctgttt ttagggggaa tgcaaaatat
     6361 tccgcaagac gtttatgagg cagccgaaat tgatggtttt tctaagtttc agcaatttcg
     6421 ctatatcacc atgccattat taaagcccac ggcgattttt attttactta ccacattgat
     6481 ttcggccttt aaactaatca ttcagccaat ggtaatgact caaggtggac caatgaattc
     6541 tactatgaca atggtttatt acatttacca aactggattt acagaccgta tggtaggcta
     6601 ctccagctcc attgcgttaa tctttaccac tatgattggt ttaattacaa ttgctcaaag
     6661 aaaattggta aaggaggatg atccagcatg accaagaaat ttggaccgct aatgatcgta
     6721 gaatattgtt tgctgttcct tttagcggca ttattcattt tcccgatgct gtggatggta
     6781 gtttcctcta tgaagcctga agcagatgtc tatcaaaatt tagcttcctt caaagctttt
     6841 ttacctagtt tgaatccagc taattggttt aaaacttatc aagaggttat ttcccggttc
     6901 agtgtgggaa cttatttatt aaacagtatt ttttatggtt taacctttgc ctgtgggtca
     6961 atcattgtaa actctttggc gggttttgcc tttgcgaagg ttaatttttc aggcaagaaa
     7021 attatctttg gagctttgtt agctctgctg attgtcccga tggagacggt tttaattccg
     7081 caatttacca tcgttaattc actaggctta gtgaatactc gcttggcagt gattttacca
     7141 gcgatggcca gcgcgtttaa tatctatctg tttagaaatt tctttatcgc gattccagaa
     7201 gaaatcattg aatcagcgaa attagatggt gcaaatattg ttaaaatttt ctttcacatc
     7261 atgctgccaa tgtctaaacc agccatagcc acagtaggtg tcttatcctt tatctccagc
     7321 tggaatgatt atatttggcc gttaatggtc ttaactgaca gcagcaaatt ttcaatgcaa
     7381 gtggcaatta ctacgatcaa tactacacag ccagtttata tcaatcaagt gatggctgtt
     7441 ttaaccattg caacgattcc actgattatt gtttatatcg ttgcacaaaa atatattctg
     7501 cagggacttg gcggttctgg aacgggtata aaataggagt gaatagtgaa tgttgcaaaa
     7561 acttttaaag gccaatgagt ttattgagaa aaatgctcaa acagtcaatc aagaataccg
     7621 gaataaattt catttgatgg ctccgattgg ctggatgaat gatccaaatg gttttgttta
     7681 ttatcaagag gagtatcact tgttttatca atattatcct tacgacagca tctggggacc
     7741 aatgcattgg ggccatgcta aaagcaagga tctaattcac tgggagcatt tgccagtagc
     7801 actggcacca ggtgaaagct atgacaagga aggctgtttt tccggcagtg ccatagaaaa
     7861 ggatggcaag ctgtatttga tttatacagg acacaatgtt gtggaagggc aagttcgaca
     7921 agttcagtgt ttggcagttt cagaagatgg cattcacttt gaaaaatatg ccggaaatcc
     7981 aatcatcgca gaagatcact tagcttctgt tgcaacgacc gaagatttcc gcgatcccaa
     8041 agtgtttcag cgaaaagaag actattttac tgtagtcgct actaaaactg cagatgctcg
     8101 tggacaaatt gtgatgttcc attccaaaga tttactaact tgggagtttt attccgtatt
     8161 gctggaggga actccagaac aaggcattgt atgggaatgc ccagacttgt ttgaactaga
     8221 tggaaaagaa gtcttgattc tttctcctat tcaaatgact ccacaaggca atgcttacca
     8281 aaatacgaac tcaactgttg ctttcatcgg tactgtcgat tggcaaacag gcaaatttct
     8341 agtagagaat tttcatgaaa tcgatggcgg tttagatttt tatgcacccc agacctgtga
     8401 aaacgaagca ggccaaagaa taatgattgc ttggatgcaa atgtggcagc gtaaaatacc
     8461 tacgaatgat ctgggccatg gttggtcagg ttccatgacc ttgcctagag agctgagggt
     8521 ggctgataat caattgttgc aacggccagt ttcatcaatt tatcaagtaa ttgatccgca
     8581 ggaaaggatt gaatgggcga agactgaaga gaatcgcttc ttactgaagg acttagtata
     8641 tgaccagcac tatttacgct tggaaatcaa tttaaaacag gcaacgaaac tagaacttca
     8701 ctatgcgaag ggacccgacc atacattact gcttaactat gatgttgaac gggagtgctt
     8761 tactgcttcc agggaacaca tgccgtatga tttatcagga gctgaaaagg aagtgttgaa
     8821 aaagcgcagt gtgacgattc ctttggaaaa taattgtcta accatcgaaa ttttccgtga
     8881 tacttcagct attgagcttt ttttaaatga tcgtctgact atgagtacaa ctttttatga
     8941 gacagaaaaa ggacaggatt ttcttttgct ggcagacagt gaagtaacct tttcattgga
     9001 aagcggctta attaatctat agaagggaga gctcagtgat gatttatggt tcaatcgaag
     9061 caggcggcac aaaatttgtg tgtgccattg gcgatgaaga aatgacgatc aaggaaagag
     9121 tgagctttcc gacaacgaca cctgaggaaa caataccttt agtcattgac ttcttcaaac
     9181 aataccaagc agatttagca gggattggga ttggctcatt tgggcctatc gatatccatc
     9241 gcgattccgc cacttatggg tacattacct cgaccccgaa attggcatgg caaaattttg
     9301 atttcatcgg taccatgaaa aaagaatttc cgatcccgat ttcttggaca acagatgtca
     9361 atgcggctgc ctatggtgag tatgtcttcg gcagtggcaa aggattgagc agtgtcgttt
     9421 actatacgat cggtacgggt gttggcggcg gtgccttaca agaagggcgc tttatcgaag
     9481 ggtttagtca tccagaaatg
//
LOCUS       DACEXQ010000093         9472 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.093, whole genome shotgun sequence.
ACCESSION   DACEXQ010000093 DACEXQ010000000
VERSION     DACEXQ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9472
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.093"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(267..1634)
                     /gene="nhaC"
                     /locus_tag="IXH04_001574"
     CDS             complement(267..1634)
                     /gene="nhaC"
                     /locus_tag="IXH04_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAQ1852180.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(1743..3164)
                     /locus_tag="IXH04_001575"
     CDS             complement(1743..3164)
                     /locus_tag="IXH04_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1852181.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(3407..5284)
                     /gene="tdc"
                     /locus_tag="IXH04_001576"
     CDS             complement(3407..5284)
                     /gene="tdc"
                     /locus_tag="IXH04_001576"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAQ1852182.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(5575..6831)
                     /locus_tag="IXH04_001577"
     CDS             complement(5575..6831)
                     /locus_tag="IXH04_001577"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAQ1852183.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            7411..8712
                     /locus_tag="IXH04_001578"
     CDS             7411..8712
                     /locus_tag="IXH04_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAQ1852184.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(8781..9380)
                     /locus_tag="IXH04_001579"
     CDS             complement(8781..9380)
                     /locus_tag="IXH04_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1852185.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT         2871 a         1956 c         1681 g         2964 t
ORIGIN      
        1 aaatacgctg accatttatc ctatggtcag ctttttatgt aaaagaagat actacttcta
       61 acacattgtt tatctgtaaa aaagagcgtg ggacaaaagt aaagaacact tttgtctcac
      121 gctctaaaca cgtataaacg gcggaagcag aagcaactcc ttcggaaata agctgaaatt
      181 cacaaaaatt taaaaagcaa ttttcttgag tttcttctta tttcttggag ttaaacgctt
      241 ctgtcccagc ctcttttttc cttccttcaa ttttttacac gtcgacgatt caataaaaaa
      301 ccgcctaata ttgtaaagac aggtgcaaga atcgagaaaa aggcgaatgg caagtaatgc
      361 aacgcgctaa ctttcatcgt tcccataatg aatgtgccgc tgactcccca cggtacgaga
      421 gaattgacgg tcgcacctga atccgctagt gttcgtgtca aataggtttt tgataagcca
      481 acgtcatcaa aagaacgctt gaatgtttct cctggaagaa tgatggacag atactgttct
      541 ccaacgatca gattgattcc gatacagctc aaggcagtaa acccgactaa acgagaaggt
      601 cggtccatct tttcttttaa ttcttgaacg atggtatcaa cgatcagata tttgatcagt
      661 aacccaccta atgccaatgc aagtaaaatc aaactagcag aacctaacat actcatgatc
      721 ccgcctcgag aaagcagttc atctagcttt ggatcaggtg tatttgcgac ataaccattc
      781 atcaagatgt ctgctgcttt tccgaccgtc aaacttttct catggataaa agaaagaacg
      841 agagcagctg tcgatcccgc tagtaatgta gggatagccg gaattttttt gattgccccc
      901 ccaaataaaa taactagtgg aatcaatgtc caaggagaga tccaaaagct cgctttcatg
      961 ttttggatca tttcattgat actagctaga tcggctgaat gactgttatg tcctaatatc
     1021 gtataaaaaa tcaacgaaag gacgaatgct gggaaatccg tataacgcat accagcgata
     1081 tgttcgaata gattaactcc tccaataccc gctgccaagt tggtcgtatc tgataaaggc
     1141 gagcagttat tccctaaaaa ggcaccggaa acgactgcgc ctgtagtcat tgcatcattg
     1201 aatccaagta aatgaccgat ccccaaaaaa gcaattccaa tcgtcgaaat cgttgtaaat
     1261 gaactgccga cagtcactcc gacaattgca caaacaagaa atgctgtcgg taaaaagaaa
     1321 cgcacagaaa caagtttaaa tccataaacc ataatcgtag ggatggttcc agataaaatc
     1381 catgaagcaa ctaagacacc aatcaaaaga aaaatcagga ttggaatgat ccctggagtg
     1441 attcctttga tgatcccgtc atggatctca tcccatgtga actttttaaa tctgccataa
     1501 aacaacaaac acatgaaagc tgcaaggatc gggatatgtg gcgagagctg gaatccgatg
     1561 atcagtactc ctaggagtgc caacaacacg atcaatacag cgatactttc ttttaaagaa
     1621 atatttttat tcatcgtcga tctcctattt caaatgaaaa ggccctgcca tgaaaaaact
     1681 attcacttgg caggacctcc tactaaatca gcgacaaaac gtcggctgaa agacttattt
     1741 ttttattttt cttctaaact tttttctacc tcaatccccc aagaatcttt cttgaagtga
     1801 ttgatgatca atggaataac gaacatgacc actgtgatag ctgctactaa taatgcatca
     1861 ctcaatgtcg ctgtacctaa tgtagcaaat acgacaacga tgatagagaa gatcagcatg
     1921 cagctgacta cccaagctaa tccgtttccg tttttaccaa ttcggtaagg acgttcgaat
     1981 tctggatgtt tcttacgcat tttaagcaat gaaacagcaa tcaaaatgta cacgatacaa
     2041 taaataacag ttgtagcatt tgtaagtgtc aagaatacag cacttacgtc atccattaca
     2101 ccataaagca aagcaaataa tgaaataacg attgattgtg ttagaacgac atttctagat
     2161 acgccgtatt tgttttcttt atggaatccg aattttggtg gaaggaatcc ttcacgagct
     2221 acttggatga ttgtttttga tggtccagta acccaagctg ataattgtaa aagcacacca
     2281 ataaatacca tgaagctgaa gatatttccg atcacttctg gtaagcctag gatttgacag
     2341 tcaatcaaga taggctgcgt gatatttgct aattccattt ttccatctgg tacgacattt
     2401 gctacaagca tcgcattgat gacattcaat aaaactaatc cgatcaaagc gataaatacg
     2461 ccttttgtat aattttttgt tgcgtctttc aaacgaggca tgtaaacaga actcatttcg
     2521 atcccaacaa aaataaatgt gataccagct aagtatttca atgaatcaat atgttccaag
     2581 tcagggaaag cttttgacca tgaaaagtca cctagatagc cgtttgagac tagtccgact
     2641 ttgaaggctg ccaaaacacc taaaacgaac atgatgacta ctgggatata tacccctagc
     2701 catacaccga tgttcccgcc gacttttgcc atatcgaatt tcaagttcaa gatcgtgatg
     2761 atccagtaga taactaaaat acaacctaaa acgaaccagt gattattgcc aagttctaca
     2821 ttaccaaatg tgtttcctaa caatggtcct aaagtagaag cgaccattac catacctggg
     2881 aacatttgca cccataatag ccatgcaacg acaaatcccc atttgctgcc cagtgctgtt
     2941 tttacccata actgaggacc gccttcttga ggaagcatcg tggaaagctc accggaaacc
     3001 attgatatcg gaccggcaaa aaacaaaact gcaaaaattg aataaaaaat caatgtccaa
     3061 ccgactgcgg ctaacgtagg aatactccga accgttgcac acagtgccat tgtcagaccg
     3121 ataaaggtcc caagacccat tttacttact tcagatttac tcaccgttga cactctcctt
     3181 gattttttgg ataaaataac ggtgtctttg aagtagtttc agcaataaac gaaacaattc
     3241 agcgaatatt tgaggggatt ctttgagttt attgcttgaa gggtttcttc aaagacaacc
     3301 tcaaaacaaa gggatcgaag agcttaggtc tccccttgct cttcagatcc aaacatgcgt
     3361 cagaaacagt tagtattcaa gctgttttct aacatgatta ccttgcttat tttgcttcgc
     3421 ttgccaataa ttggtctgcg tagatttttt ccaatttttc ttgaagagct gctttgattt
     3481 tttcagcata ttcttcaaag tgttcttctt tgttcatgta tggtgtcatt acgctggcac
     3541 gcaatacagt tacttttccg gcacggttcc attcttcatc tgagaatcct agttggttca
     3601 caaattgtaa tgggctgttg ccatagtctg ggatagcaaa gtcagtatgt gaagttaaga
     3661 actcattacc gtaaatgctt cctttcacgt atgaagaata atcatatacg tcatggttca
     3721 atttattcat tgcaactaag tcatcgttgc ctttttcttt gaatacatag tctaccatat
     3781 tgaaatctgg ataagttaat ggatgaactt cgatttcttt gtctcctact ttaaatgaca
     3841 agtcattcaa gaagttgtag aaacgatgtg caccttcgat tgatgcaccc attaatttac
     3901 catatcctgt gacattcaaa ggtaagacat ggtgtgctgc ccaaacgctg gctgctgttg
     3961 cccctgcttt tgaaccttcc aagatataag ccccaagtaa tgctgggata tctgcgcctt
     4021 tttcaaatac atatgtcgca aagtaagaga taacgtctct catgcggata tctttgatga
     4081 cgataccacc ggcagaatat ggaacatagc ccattttatg tgggtcaatc gttacagatt
     4141 ctgcttcttc gattgcttta tatgcactgt gtacttcttc taagatataa tctttgtttt
     4201 ctgtaaagac attgtatttg taatgaacat cttttaaatc ttcaaatgga atgaagttgt
     4261 tgtcttcgtc taagaaaatc gcacggccgt atccgccata agcagcatct acgtgtaagt
     4321 agaagtaaat accgtctttt tctaatacgc gacgcaatgc aacgatttta tcgataccgt
     4381 cgattgctcc ttcttctgtt gatcctacaa caccgactac acctaggatt ggtgtttttt
     4441 cagctgctag accacgaacg attttttcaa gttcgttgat gtccatgcga tagttgtggt
     4501 caactggcac tggaatcact tggtctaaac cgataccgat gatgtcggca gcttttagcc
     4561 atgaatagtg tttagtttgt ggaaccaacc attttcctaa tttttctaaa tgttttccgc
     4621 tacgtgctga gtgagctttg atttcgtcga ttttttctgg gacgctgtct aacagattca
     4681 tgatttcttc tgttgacaag ttcattaatt cccaatcgct ttttccagca actagttcag
     4741 gtgtcacttc tttcattgca agcggcaatg atttgatgtt gcgggcatac caaagtcctt
     4801 ctaagtttgc taaagaacca tcagcaacga tgtggcccca gccatcttta tagctcatta
     4861 attttgcaaa ttccattcct acttcttctt ccatttggct tgttgcagga gaagattcat
     4921 aggcaacgtt attaccattc catagcatag caaaattgta cgctagtaaa gatggcatca
     4981 atgtttcaga gttcatatga ccccagtaac gtccggcatt atgccaagga acggaatggg
     5041 tacgcatccg cgctgaaatt tctgatagaa catcctttgt tttattcaca gtgtgttcaa
     5101 aactagcact gcttttttct tctggcgtaa taataggcat gtcttgaggc atgtagtttt
     5161 gacgccagcc taaatgttca tccaccaatt cattcaacaa agctttatag atttggccgt
     5221 tctcagcttt gtccccaatg aatagggcat ttaggtttag atctttcgac aatgattcac
     5281 tcatagtgaa accctcctaa aaaaatattt aaattttgtc gagccctaca ttacacctgt
     5341 tcttctattc tgtaaactaa gttttggcct gatttttaat catattttaa tatgtatcaa
     5401 ttatccgaat tgttcacact tttgaaataa ttaaaaaaaa cgagggaatt tacaaatgta
     5461 aactccctcg caaagattgt attaacgaca accagcgttc tattttagga tgggtttcaa
     5521 aattggaatg ttgtaatagt atttcccttt ttcaaggtaa aaacttacta aattctaatc
     5581 aattactttg gccaaaaagt aatttttctt accttttcga ataaccacaa acttaccatc
     5641 gaattcatcg gatggattta tgacaaaatt taaatcggta acgcggtcac cgttaatact
     5701 tatagctccg tttgaaacat cttcacgtgc ttgacgttta gatggttcaa tttttgtgga
     5761 aacaagcagt tccacgatat tttcaggtgt gcttgaaatt tcaacgttcg gcattttacc
     5821 gaatccttgc gcgatttctt ccgcatttag ttctttaata tttccagaga acaatgcttg
     5881 cgttattttt tgcgcttctt tcaagtcttc ttcactatgc acaaatcttg taacttcttc
     5941 tgccaaacgg cgttgtgctt cacgtttctc aggttctgtc tctacttttt tcgctaagtc
     6001 ttcgatttct tcttgagaca agaaagtaaa gaatttcaag tatttcacta catcgcgatc
     6061 atcttgattc aaccagaatt ggtagaactc aaatggtgaa gttttcttcg gatcaagcca
     6121 aacggcaccg cctgcagttt ttccgaattt cgttccgtca gccttcagca tcaatgggat
     6181 cgttaaaccg aacgcttctg cttctggtcc ttctaatttt cggattaggt ctaatccagc
     6241 tgtgatattt ccccattggt ctgctccacc gatttgcaat tgcacatcgt atgttttatg
     6301 cagatgcaaa aagtcgatag attgcaggat ttggtaagta aactctgtaa atgagattcc
     6361 tacttccaaa cggctggcaa cgatatcttt tgctaacatc gtgttgatgt tgaagttttt
     6421 accgtagtct ctcaaaaatt caagtaaaga gatttttgac aaccagtcgt agttgttcac
     6481 aaatgtgata tttgcatctt taccaaacaa ttttctcatt tgattggaaa gtgcgtccac
     6541 gttatgttgc acttgttcca tcgtttgcaa cacacgttct gttttgcggc cgcttgggtc
     6601 gccgatcgtt cctgttccgc cgccaatcag aatatatgga tgatggcctg ccaattggaa
     6661 tcttttcatc atcataaatg gaatcaaatg tccgatatgc atcgaatcac cagttggatc
     6721 aacgccgcaa taaagtgaaa tgctcttttc ttcaacgagc tcgcgaagtc tttcttcgtt
     6781 tgtttgctga ttaatggcgt cgcgccaagt taattcatca ataatattca ttttactgac
     6841 ctccgtgtag tttttttatg aaagcaggaa cctgctagca tgtgtaatta attatttctt
     6901 caggatagga taggaaataa aaaaagtccc ttggcatata agccaaggga cgaaatctcg
     6961 cggtaccacc caatttgtat gtctagatat ccaacatacc tctcgaacat ttaacgctgt
     7021 cgcgtggcaa tcactgccaa agactcctga aatgtaattc gctttaaagc atgtactggt
     7081 ttgcagcgac caccagcttt ctgaaacatt ggctttaaca actacttcgt ttcgatcttc
     7141 gtcttgttat tattaatatg tgtctatcat agagcgcaac tttttctttg tcaacagaaa
     7201 aaggatattt tttttaaatt cctcattttt tcttttttga atccgctcta attccgaaca
     7261 ttttattttc aattattgtt aaaagaaata actttttctg tattttttct gcaatttatc
     7321 gagaagtcac tttactttgc ctcttattct ttcttcgtta caataaggaa tgggacaagt
     7381 aaaaaaatga aaaagcaagg gacgaggtat atggaaaaaa agcaaaaaat tattttagga
     7441 gccacttctg ctttaactat cgttatttta attctaagtt tgttttttgt catccgatca
     7501 tctacaaaaa aagaaccaat cattcctgaa aaaaaaataa cagaaaaagc aaaagaaccg
     7561 acagatgata agcaacaact agttactcaa gcaaaaaatc tggctcaaag ctacgattat
     7621 gaccaagcga ttgcgttatt ggaaaaagat agtaagaacg gatctactga aacacaacag
     7681 cttcttcaaa cactaaaaaa ggagaaagaa caactgatcg actggtctga tcctactcag
     7741 atctctcatg tctttttcca cagcttgatc gtggacccgc aaaaagcctt ccactctcaa
     7801 caggcacaag gatacaaaga ttatatggtg actgttgaag aattcaaccg ttcgatcgaa
     7861 caactttatc aaaatgattt tgttttagtt aatttgaatg atttgatcca aaaagacgaa
     7921 caaggaaatc tttcctttaa cgggcttgcg ctacctgaag gaaaaaaacc attggttctt
     7981 tcacaagatg atgtcagcta ttatgaatat atggataatt ccggttttcc tagccgattg
     8041 gtcgtatcaa ataacaatga aatcaaaaat gtctataaag agaaagataa agaagaaatc
     8101 ggtgactttg acatggttcc tctgatcgat gcctttgtca agaaacatcc tgatttttct
     8161 tatcgaggcg caaaaggtac cttggccctg acaggttaca acggagtttt aggttatcga
     8221 acatcaaaat ctcaatatgg agacaatgaa aaaacaaaaa aagagattaa agaagctaaa
     8281 aaagtggcgg aacagttgaa gaaagacggc tggagctttg cttcccatac atggggtcac
     8341 ttgaatatga ctcaagcttc actagaagat gtaaaaaaag acaatgaatt gtggcagcaa
     8401 gaagttgcac ctatcctagg aaaaacaaat attctgattt atccatttgg tgcagacatc
     8461 agcgactggc agccttattc agcagaaaat caaaaatttg cttacttgaa gagtcaaggt
     8521 ttcgatattt tctgtaatgt cgatgcttct acaccagctt ggggacaatt aggtacggat
     8581 ttctatcgaa atgctcggat caatatcgat ggcattcgtt ttcaatctga tttagacgga
     8641 aaaaatccta ttcttgatcc attcatcaat gtccatgatg tctatgacaa agaagcacgc
     8701 gaatctgctt gattttacgt catttatgaa ccaaaaaagc cgaactgaca ttttatcgtc
     8761 agtttcggct tttttatgct ttatttcttc ttcggtgcga ttcccttgat caggacttct
     8821 gtccattctg ctgtatatgg aagcagtttt gcgtatatct ccggcttaga aattccttct
     8881 accattgctt gaaaatagag gatcaagaac tcatccgaat atttcaaatc cacttttcct
     8941 tctttccgcc ctcgatggaa aagatcaagc atgatactga aactttcttg cgtatacgca
     9001 tccatcgcgt cagaaaattc ttttttttct ttttttgtat agtatgtcat caaatcgata
     9061 taaaattgtt tgttcactcg attgagataa cggattttgt tttcactcat ggcaatcagt
     9121 gtttcttcaa atggaagatt ttccgcaatc aacgctttcg cttcttgccc taaatcattc
     9181 ataaatgcaa aaaatacttc ataaatcagc ccttccttgc tgccaaaata tttaaaaatc
     9241 gtcgctttac ttacctgagc gttctcagct accttatcca ttgtcactcc agcggttcct
     9301 atgtctgtgt tcatcaagtc aaaagctgct tgtaaaatct ctttcttttt ctcttgtgct
     9361 ctcttttcaa aaccgttcat ttgtagagcc tcccgtaatt ttaaactttt ataatatata
     9421 tagttcaaat ttattctgag gtttattata actatctatt gagaaagtat ca
//
LOCUS       DACEXQ010000094         9365 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.094, whole genome shotgun sequence.
ACCESSION   DACEXQ010000094 DACEXQ010000000
VERSION     DACEXQ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.094"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            286..435
                     /locus_tag="IXH04_001580"
     CDS             286..435
                     /locus_tag="IXH04_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAQ1852186.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            446..1960
                     /gene="dltA"
                     /locus_tag="IXH04_001581"
     CDS             446..1960
                     /gene="dltA"
                     /locus_tag="IXH04_001581"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAQ1852187.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1957..3162
                     /gene="dltB"
                     /locus_tag="IXH04_001582"
     CDS             1957..3162
                     /gene="dltB"
                     /locus_tag="IXH04_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAQ1852188.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3207..3440
                     /gene="dltC"
                     /locus_tag="IXH04_001583"
     CDS             3207..3440
                     /gene="dltC"
                     /locus_tag="IXH04_001583"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAQ1852189.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            3443..4711
                     /gene="dltD"
                     /locus_tag="IXH04_001584"
     CDS             3443..4711
                     /gene="dltD"
                     /locus_tag="IXH04_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAQ1852190.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4852..5934
                     /locus_tag="IXH04_001585"
     CDS             4852..5934
                     /locus_tag="IXH04_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAQ1852191.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5934..6737
                     /locus_tag="IXH04_001586"
     CDS             5934..6737
                     /locus_tag="IXH04_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ1852192.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6807..7547
                     /locus_tag="IXH04_001587"
     CDS             6807..7547
                     /locus_tag="IXH04_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ1852193.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7558..8562
                     /locus_tag="IXH04_001588"
     CDS             7558..8562
                     /locus_tag="IXH04_001588"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAQ1852194.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8607..>9365)
                     /locus_tag="IXH04_001589"
     CDS             complement(8607..>9365)
                     /locus_tag="IXH04_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ1852195.1"
                     /translation="QTTINWSTILYIIILIVIISYNLIDHILNTDKDRRQVDFLKIKN
                     DLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEKILEEAKNFEM
                     LCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDDLAQLLIHALEHSFR
                     ETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGITEELDDQERFDLMMM
                     KDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         2982 a         1541 c         1915 g         2927 t
ORIGIN      
        1 aatgatatac ggaataagac aagataataa atagaactaa attttgaata ccatttattt
       61 ggaataagag gttgtgacac gatttttgtc acaacctctt tattttttat attttttaaa
      121 agtacgagaa attataaaga aaaaagcagt gatttgatgt gctattttta taaaagctat
      181 gtgacaaaac tgttagtaaa aaaacggttt ctgtaagcta gacacatgtt tctttttgtc
      241 aaaattagtt acactttgtg tgaggttata tgaggaggga atccaatgaa caaatggcgt
      301 tctaatccaa aattgaatta ttggggaaaa ttcatcattc gaacaatttt ttatagtgca
      361 gtactgctgt tcctgattta tctgtatcac tataaaaata ttcaaggagg aacttttatc
      421 tataacgaat tttaggagga agagtatgga aatcaaaacg attattgaag caatcgatga
      481 ttggggaata aatgaacctg atcgagttgc ttatcaggcg gatgagacgc atacttttgg
      541 tgaattgaaa caagcatcag acgcacttgc ttattattta gaaaaaaagg tagaaggcga
      601 tggaccaatt gtcgtttttg gcaatttgga gtttgaaatg attgtctcat ttctaggggt
      661 tgtcaaatct ggccatgcct atttgccgat tgaagagcat acacccaagg agcgtatcct
      721 atcgatcttt cgggtggcta aaccatctat ggtcatcagt atcggggact ggatagaaga
      781 tattccttcg gttcctgtca taacaaaaaa agaatttcaa gaaatcaaga agataccagt
      841 aggattttat cccagaaata gcgtaaaagg tacagaaaat ttttatatta tttttacttc
      901 tggtactact ggagaaccaa aaggcgtaca aatcagtcat gataatctag tcagctttgt
      961 tcaatggacg atggaagact ttgggatcaa gcctggtatg catttcttag cccaagcgcc
     1021 attttctttt gatttatcgg tattttctat ttatcctgca ttagtttctg gcggaatgct
     1081 gaagccgtta aataaagcag ttgttcaaga ctttcgtcaa ttattcgcga cattgccagc
     1141 cttgaagttg aatgtgtggg tttctactcc atcatttatg gatatttgct tgatggaacc
     1201 tacattcaat ggagaaaatg tgccggaatt ggacatgttt ttattttgcg gagaagaatt
     1261 gacgaaaaaa actgcagaaa gtctacttga tcgattccca aatgcacgga tatataatac
     1321 ttacggtcca acagaagcaa ctgtagctat ttcaagtatt cagattgacc agcatgtact
     1381 agatagttat gaccgactac cgattggcta cgtaaaaaaa gacacgaaag tttctattgt
     1441 ccaagaaggt aaacctgttt caaaaggaga aacgggtgaa atcattattg caggaccaag
     1501 cgtatcaaaa ggttacctga acaatcccga aaagacagct gctgcgttct tttgtcaaaa
     1561 tgacttagca agttaccata caggggatgc aggtagacta gatgaatatg ggcttttatt
     1621 ttatgaagga cgtatggatc agcaggtcaa attacatgga taccggattg aactcgggga
     1681 tatcgaacat tatctgctcc aagacaaccg aatcaaacaa gctgtcgtcg taccaaaata
     1741 ccaaggaaca aaagtacagc agctggttgc ttttgtcgta ttagaaaaca aaacgaaaca
     1801 gccggatttt cagttgacta aatctatcaa agaacaatta ctagaagttg taatggatta
     1861 tatgattcct cagaagttcc attatgtcga tctgctgcct cagacagtta atggaaaaat
     1921 cgatcgaaaa aaattaacag ctgaggtgaa tcctgaatga tgttacctca tatgatccct
     1981 tatgcagagc cgttttattt ttttctttta gccattgcgc ttgtaccgat cgttctatct
     2041 ttattgatat gggaaaaaag attaccagtt tatcaatcag cagtgacact tttcttttta
     2101 tttgtcagct ttggaggtaa ttattggcaa cagggagcgg cgctgattgg gtatgttttg
     2161 tggcagacga tacttgtatg gggctatttt gtttatcggc aaaggaaaaa tcagacagga
     2221 tggttttttg ccgccgttat tttagcgata attccactgt ttttaacgaa agtgactcct
     2281 ttttttgaac atggaaaagc atcgttactt ggttttttag gaatctctta tttaaccttt
     2341 aaatctgtac aaatgatcat ggaaataaga gatggcatga tcaaaacata taagccattt
     2401 cgttatatcc agtttctgct gttctttcca acaatctctt ctggaccaat cgatcgctat
     2461 aggagatttg aaaaagatca gctccatccg cctgttcgtg ataaatatgt tgatcttttg
     2521 ggaaaaggcg tccaaaatat ttttgtcggt tttttatata aattcattat cggttattat
     2581 tttggtcaag tactgcttcc tgtagtggga cgcattgcct tacagcatgg tggtatttca
     2641 tgggcattag taggttatat gtacgtgtac agtatgtatt tgttttttga ttttgctggc
     2701 tatagcttat ttgcagtcgg tacaagctac atgatgggat atcagacacc aatcaatttc
     2761 aataaacctt ttttatcatg gaatatcaaa gaattctgga atcgttggca tatgacgctg
     2821 tccttttggt tccgtgatta tgtatatatg cgattgatgt tcttcatgat gaagaaaaaa
     2881 ttttttaaaa gcaagatcgt aatgtcaaat atcggttatt ttgctttgtt tcttctaatg
     2941 ggtatatggc atggattgac gtggtactac atcgtatatg gactgtatca tgcttgtctg
     3001 atctgtctga cagatgcatg gatccgattt aagaagaagc ataaagagca gttgccttct
     3061 aacaaattta cgcatggctt agccgtattt ttgacatttc aagcagtatg ttttagcttc
     3121 ctgatttttt caggattttt agataaactt ttttttagct aacgaatagt gacatattaa
     3181 tttatataac aaaggagaaa aggaaaatgg aagaacaaat tttatcaatc ttagaagaaa
     3241 taacaggaac agatgaagta agaaaaaatc gtgatattga tctatttgat gaagggttgc
     3301 ttgattcttt agcatctgtt caattattag tcgagctaga cggagagctg gatatccaag
     3361 tccctgtatc tgaattcgag cgagaggatt ggagcacacc gaataaaatc attcaacaag
     3421 caactgcatt gaagggttga gtatgaaaaa gaaacttttt gctattttcg gcccggttgt
     3481 tttagctgtt ggcttactgt ttgtgttttt tacctcctcc tatcaagtag atatctctaa
     3541 tccagaaacg attagacgtg cctcttcttc catgtcagtg aacgtgttga aaggagatgc
     3601 gataaaaaat tatgcattat ctgaaaaaca atatattcct ttttttggct caagtgaatt
     3661 aagcagaatc agtccatttc atccgtctgt tctggcagaa aaataccagc gaaactatcg
     3721 tccatttttg ctgggagcac ccgggacaca atcgttaagc caatatatga tgatgcgttc
     3781 tgcaggagat gctatgaaaa acaagaaagt cgtctttatt atttctcctc agtggttcgt
     3841 taaaaatggt gtaaaaactg attactttaa tacgtattat tctgaacttc aaacatacga
     3901 ctggttgttt tcaatgaaga aagtaacgcc tgctgatcgc tatttagcta gaagattatt
     3961 aactttctca aaagtaaaag agaatgatac gttgacggcg attctgcaaa cgatcaaaaa
     4021 agggaagcta cctttacctg aatctttgaa tcagctgcgc agccaatgga acatgctgaa
     4081 gagagaggat gaagtattca gcaatattgg gttgaaagat cgacagcaaa aaatcgatca
     4141 tgagtcaaaa cgattaccaa aacaatacca agaaacagaa ttatccattt tagcaaatca
     4201 aattggagaa cgagaaacga cgaataatcc attcgggttg aaaaacgatt tttatacgca
     4261 tcgtatccga gcccacgagc cagagctcaa acagtcacaa aaaaattggg attatcgttt
     4321 ttcgccagaa ttttctgatt ttcagctggt cttggatcaa ttagcaaaaa accataacga
     4381 agtattgttc atcatcccac cggttaacga gaaatggagc gattataccg gactatctca
     4441 agaaatgtta caaggatttg ccaaaaagat caaatttcaa ttaaatagcc aaggattcaa
     4501 tcgaatagct gattttgtca atcaagcggg taccaactat tttatggaag acacgatcca
     4561 tctaggctgg aaaggctggt tggctgcaga tcaacagatc agaccgtttt tagaagaaaa
     4621 tcatattact gctagtaagt atcatcttga cgatgctttt tttagcaaat cttggcaaca
     4681 tcaaatacca gataaactac aattaaaata aagaaaacag gcgagtgttg ctcgcttgtt
     4741 ttctttattt ttgaatattc tgaataaaag ataaaaatac caagtttgcc tgaacaaaaa
     4801 tcataagcaa tggtataatc acaatatgaa tatagaaatg ggggaaaaca tttggataca
     4861 aaagaagaac agctacttat tgatttaact tctgcaaaag gagtacctgg aaatgaagaa
     4921 gaggtaagag aagtattcag agaatatgcc aaaccattcg ctgatgatat tttttatgac
     4981 ggtttaggca gtgtgattgc caaacatggt gctggcggag gccccaaggt ctttatttct
     5041 ggacatatgg acgaagttgg ttttatggta acaaaaataa ccgaaaaagg attcttggaa
     5101 ttccaaactc ttggaggttg gtgggggcaa gtcatgcttg ctcagcaagt tgaaatcaaa
     5161 acacgtgaag ggaaaatcgt ccacggtgtg attggttcca aaccaccaca tgttttaaca
     5221 cctcaagtaa gaaataaacc ttatgaaata aaggatatgt tcattgatat tggcgcatca
     5281 agccaagaag aagccaaaga gtggggcata cgtcctggag atatggtcac tccatatatt
     5341 gaatacaaac gaatgaatgg cagtaaatat ttactggcaa aagcatggga taatcgtatc
     5401 gggacagctg tttctctgcg agtattagag aatctatcta aagaagcaca cccaaatgtt
     5461 ttattcgcag gaagcgatgt gcaagaagaa gtaggcttgc gaggtgctcg aacaagtaca
     5521 caccttgtaa atcccgatat tgcttttgct ttagatactg gtacagcagg agacactcca
     5581 ggaatgacac caaaagaagc agattcgatt ttaggaaaag gcccacagat tttgattttt
     5641 gatgcatcta tgatcccgca taaaaaatta ttgaattttg ttattgatgt agcagaggaa
     5701 atggatattc cattccaata tacagtgatt tctggcggag gaactgatgc aggacaaatg
     5761 catttgacaa gagatggtgt tccgtctgta gccattaccg ttcctgttcg ttatctgcat
     5821 tcccatacat cagtgattca tgaagatgat tatttgaata cagtgaaact agtcacagaa
     5881 ttagtgaaac ggcttgatag tgaaacagtt gaaaaaattc ggagttatga ataatgaata
     5941 atagaatacc tatttctgaa tatcggaaaa aagcacgagt ttctttggaa aatcaatggg
     6001 gaatcaatac ttggcttgtt ttcttatcta taatatttac gagcgtcatc cagaactttt
     6061 ttgacggttt cagtcctttt ccggaagaaa gcatgcagca gcacttattg agttttctag
     6121 tcgataattt acttttattc gcattcaact atgcattgtt ttacattgcg ctagtagttg
     6181 tacggggtgg cagaccaaaa agtaaccttt tattttcagt tttccaaaga aagtattttg
     6241 gttcgatact tggcatcaat ttgatcaatt cagtcatcaa ctggctactc aacagtttaa
     6301 tctttctgcc catgttttta atcagcggag tgagtactta tatgcagctg cttttcagtt
     6361 caaatgcggt acctacgggt gtcatgccag aagattttct tgatatcagc tttgttttat
     6421 cgatgatagg gatgattttt gtttcagcac tcatcatgag tattgttggc ggtttattcc
     6481 aatttgctgc atggacaaag ttcgactatc ctgatttgtc ggttattcaa tgtttgaaat
     6541 atggctggtt tttattgaaa gatagaattg gtacgtatat tttgttgcag ctgtctttta
     6601 tcggctggta tttgctagga gcaatcgctt tgttcatcgg tcttttgtgg gtcattgttt
     6661 atgtgaatgt gtcagttgca gaattttatg aacaagcacg gattgaaaaa ggaggtccag
     6721 acgcctattt tacttagcat gtaagcagtt tattcatcaa aaaaataact tttgtggtag
     6781 gataaatctt tgagggggaa tgtacgatga aattaactgt tttaggatgc ttaggagcgt
     6841 atccttataa aaatcaaggg acaacaggtt atttgcttca atctgacggg ttcaatttgt
     6901 tgatcgatgc aggtagtgct acgctgatca aattacaaga acaccttgat ccacttgatc
     6961 tagatgctgt gatccttagc cattatcatc atgatcatat tgctgacctt ggtgtattac
     7021 agtattactg gcaacttcat cctaagagaa atccggagac attgctttca atttatggac
     7081 acacaaaaga ttccattcac tttgaagaat tgacgttgga aaatgtctca aaaggacatc
     7141 cttattttga agctgaagaa ttaaaaattg gtccttttct gattactttt atggaaacaa
     7201 tccatccagt agtatgttac gccatgcgta tcgtagaaga aacaacgggg aaagtattcg
     7261 tatttacagg tgattcaggt tatttggaaa gtttcgctga ttttgcgaaa gatgctgatt
     7321 tgtttctagc agatacctat ctttttgaag gaaatgaaca tcatcatgcc catttcactt
     7381 caaaagaagc tggagaactt gcgaaatcag ctaacgtaaa gaaacttgta cttacacacc
     7441 tacctcagtt tggtgattta gaacaattga aaaatgaagc gcaaaaggca gcaggcaaag
     7501 ggattgatat agaacttgct gcagttgaca aagtgtttaa catttaggag gaagaacatg
     7561 atttttgtac caaatgaaaa ccacgaccca agaatcaatt tagcaattga acaatatttg
     7621 ctgcaagaaa tgcctttaga tgaacctatt ttgctctttt atatcaatga accttcaatc
     7681 atcatcggca gaaaccaaaa tacgcttgaa gaaatcaatc gagattatgt ggaagaaaat
     7741 ggcattcatg ttgtccgtcg cttaagtgga ggcggagctg tctaccatga ttttggaaac
     7801 ctgaacttta gttttatcat gcctgatgat ggggattcat tccgtgactt tgcgaaagta
     7861 acacagccaa tcatccaagc acttcatgaa ttgggtgttg ctggggcgga attgaaaggc
     7921 cgaaatgatc tagtcattga tggcatgaaa ttttctggca acgcgatgta tgctaccaac
     7981 ggtcggatgt ttgctcatgg aacaattatg ttcgactctg atatcaatga agtagtcaat
     8041 gcattaaaag taaaaaaaga caaaatcgaa tcaaaaggaa tcaaatcgat ccgttctcgt
     8101 gtaacgaata tcaaaccatt tcttccagaa gaaaagcaaa aaatgacaac agaagaattt
     8161 cgtcaagaaa tcttgttgaa aattttcggc gtagagtcag tagaagaagt aaaaacgtac
     8221 gaattgactg atgaagattg gaaaaaaatc aatcgaatct ctgaggaata ttatcgaaat
     8281 tgggattgga attacgggaa gtctccagat ttcaatttct ctcgccaaaa acgtttttct
     8341 atcggttcga ttgaagtcca tttgaatgtc tcagaaggaa tgatcaaaga agcaaaaatc
     8401 tttggcgatt tcttcggact aggagatatc tctgacgtgg aaaaaaaatt ggttggacag
     8461 aaatatgata aagcatctct atcaaaaata gtcagccaaa ttgatgtgaa gaaatatttt
     8521 ggtgcgatag aaccagaaga attgcttacc ttactttatt gaaaataaaa aatagcggaa
     8581 tccacatata tggattccgc tattttttat acaggattcg ggtaatttcg agagagttga
     8641 acagtgatcc gatcaatgaa agtatctttt tcctgcgtta gtgttcctgt gtaacgatcc
     8701 gctacgtctt tcatcatcat taagtcaaaa cgttcctgat catctaattc ttctgtgatg
     8761 cctcgactaa ataggtttgt atcgtaatgt gcactatgtt cgcaacaaat gacgaaatgt
     8821 tcctcttgtt cgtgtatcga caaataaatt tttcgataca gtggattttc gatggcatga
     8881 gtttctctaa aggaatgctc taaagcgtgg atcaaaagtt gagccagatc gtcatcgttg
     8941 ataggaagtt cttcgggaag atctgcttgg aaggttatct gaatattccg tttggcagct
     9001 aacttctggt aacgcgctaa gataagattt gtcacctggt ggttgcaaaa agaacacagc
     9061 atttcgaaat tttttgcttc ttctaatatc ttttccacat agtttttagc ttttgtttgg
     9121 tccgtatgca ttaaatcttg cagattctcc atctcttgga caaattccag gcgaagtgta
     9181 tttacttctt ggatatgctg tcgaagctgc tcataatgtt gctccaacaa atcatttttg
     9241 atttttaaga aatctacttg tctccgatct ttgtcggtat ttaagatgtg atcgattaga
     9301 ttgtagctga taataacgat tagaatgata atatataaaa tcgtagacca attgatcgtt
     9361 gtttg
//
LOCUS       DACEXQ010000095         9317 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.095, whole genome shotgun sequence.
ACCESSION   DACEXQ010000095 DACEXQ010000000
VERSION     DACEXQ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.095"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..2122
                     /locus_tag="IXH04_001590"
     CDS             <1..2122
                     /locus_tag="IXH04_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852196.1"
                     /translation="KKINAVLSLFVCITILFFSTNVINGIKTSEALYKNTAEIHMQYK
                     DDMDFNSYINALYEISTQENINISQYSFTAVNQLTILATNPNANKHWKFSKKNILARN
                     DSKVHYTNNKNSKYQLKLPNNFLNINIYPFSHVENIGLSEILYVQGNSNKLIPVLKKY
                     GTPTISNINQSDTFQINTNIVLVICYLSIFIVITTIIFAFSKMKEITLKKMLGYNSFD
                     TIKDSIKELNLLFCSLIITFILSIFLSIYYSNENFFLSFLLCSIFHFIISFLFIIVTL
                     SIYHLSSIKNYLKNSRPLKLNLCILNICLFLSMILLLIASTKVINIHNEAENNSLKYW
                     ERTTNLYKTNITNQLNRNNTVEENNYLKKASKFVYKIQKNYKTFIIAPYNYATIQENN
                     KEFFIGQKVYPNFEDYVSQPAGAGICVDLNYLKYYNPISFEESTDLNLINSDPLTTYI
                     LVPKKYKEYAKMIEKNYLEDIQFRLEDSPPQAPYKTPNLKNLKIKLIFVNNNQKYFSF
                     NTLYGKAKDNYTITDPIVRVINPEITESLFWGNILTSDGGLFFDQKHTSNQNFFNQIN
                     PYIKEFNLENIINFSYSVFQETGELLSQKKVNKLNAYLQYLLILGLFIIINFQMIFVS
                     FTINSKKIALKKIFGYSPISQVLDLCLLPFTIELFATMCFQLIFSKNTYLYFIIFMIF
                     LINLITSIIIYKKNSFKYFKENIL"
     gene            2119..2739
                     /locus_tag="IXH04_001591"
     CDS             2119..2739
                     /locus_tag="IXH04_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1852197.1"
                     /translation="MILSVENFTLSINKKKIKQNWNFSISEKDFIIVTGKSGSGKTTL
                     LNNLSLIDKQYTGNLFYFEKKVSKHDIQRLRRNHISYLFQNYALLENQTVQYNFELAK
                     KFNSNISNDHIYNLLTDFSLPKDILKEKIFLLSGGEQQRIALIRNMLKPFDILFADEP
                     TGNLDKENSTFIMNFLKNLTEKEKKAVVLVTHDLNVLNYGTNIIEL"
     gene            3432..3812
                     /locus_tag="IXH04_001592"
     CDS             3432..3812
                     /locus_tag="IXH04_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAQ1852198.1"
                     /translation="MSEQNQNLASNSSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            3794..4708
                     /locus_tag="IXH04_001593"
     CDS             3794..4708
                     /locus_tag="IXH04_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAQ1852199.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQTAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKK
                     KVRSNRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTP
                     EQPEIRPARRNHEQHVERGTDHQQELSL"
     gene            4948..5637
                     /locus_tag="IXH04_001594"
     CDS             4948..5637
                     /locus_tag="IXH04_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852200.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFL
                     GCIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQALAQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYVKDTKI
                     EEACSDLFGDTNPWFASEEEKKEKQQSVTYERYEILLQQATNMQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(5758..5844)
                     /locus_tag="IXH04_001595"
     CDS             complement(5758..5844)
                     /locus_tag="IXH04_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAQ1852201.1"
                     /translation="MFELFICPLLVGVCVSLFSYWLNNRNKK"
     gene            6489..7226
                     /locus_tag="IXH04_001596"
     CDS             6489..7226
                     /locus_tag="IXH04_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ1852202.1"
                     /translation="MSNDIVRYNNGLNSVPLRKFTPIDMDLFWGICSKMKRKGTKEEI
                     FTFKQIKELIDYNINARGDRFANDLIKMSKKIITLSFIYEDEKTYEQLNLFQKFKIDK
                     VNETLLIKVSEEFEYILNSIGTNFTRFELENMTNLKSSYVKELYRQLMAHKDRRSKKG
                     HWFVKIEDFRALLAIPESYRMRDIDKQIFAQAKKEFLNVNSTHPAIFKTFEIKKIKAY
                     KGNKIGSLQIYFEENIPSITLDNWLEK"
     gene            7254..7793
                     /locus_tag="IXH04_001597"
     CDS             7254..7793
                     /locus_tag="IXH04_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAQ1852203.1"
                     /translation="MQPKETFTIKELSELFEISRQAISKHVDKLDPSLLAKNERGYKV
                     VLRSGVLQLASNLGNQRLLYRLHEYEQENDKNVYISSKNVIVNELLDKLREENEFLRE
                     QLSLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLAYTEETPEDESTTILSEET
                     ESIEKHTKKKKQKWWQLWK"
     gene            complement(8291..8752)
                     /locus_tag="IXH04_001598"
     CDS             complement(8291..8752)
                     /locus_tag="IXH04_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1852204.1"
                     /translation="MEDLGSKLRELRVKKGYTQEYVARKFNVTRQTISKWENNKTTPD
                     IETLKKICEFYSTNINSILNEDNLKNKQNNLLVLTAIIFNIFVTILGLFELIQMNFFS
                     FTHGIFTWFALSGLIVIDILNYFYIYYSYLKNEKFFYTIFFPLVILIILLS"
     gene            8885..9259
                     /locus_tag="IXH04_001599"
     CDS             8885..9259
                     /locus_tag="IXH04_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HAQ1852205.1"
                     /translation="MKKFLCLSALMGVLLTSGGIVSATEALNLDVPEDHTAIYGGGME
                     ATEEGFSSSKLRYAAGGGDFNCGVNGFKVYANYYHARAKHSATAKNGRGGQVRSVQKA
                     GVRAYATCNATLTGNTGWWNVY"
BASE COUNT         3602 a         1423 c         1452 g         2840 t
ORIGIN      
        1 aaaaaaaata aacgctgttt tatcactttt tgtctgcatt acaatactat tcttttctac
       61 taatgttata aatggtataa agacttctga agcgctatat aaaaataccg ctgaaattca
      121 tatgcaatat aaagatgata tggactttaa cagttatata aacgctttgt atgaaatatc
      181 tacacaagaa aatattaata tttctcaata tagttttact gctgttaacc aactaactat
      241 tcttgctaca aaccctaacg ctaacaaaca ctggaaattt tctaaaaaaa atatattagc
      301 aagaaacgat tctaaagtcc attatactaa taacaaaaat tctaagtacc aattaaaact
      361 tccaaataat tttttaaata taaatattta tcctttttct catgtggaaa atattggatt
      421 aagtgaaata ttatatgtac aaggaaactc aaacaaatta attcctgtac tgaaaaaata
      481 tggaacacct actatttcca atatcaatca atcagatact tttcaaatta atactaatat
      541 tgttcttgtt atctgctatc ttagtatatt tattgtaatt actacaatta tatttgcttt
      601 ttcaaaaatg aaagaaatca ctctaaaaaa aatgttaggc tataactctt ttgatactat
      661 aaaagattct attaaagagt taaacctctt attttgttct ttgattataa ctttcatttt
      721 atctattttt ttgagcatat attacagtaa cgaaaatttt ttcctatcat ttcttctatg
      781 tagtattttt cattttataa ttagtttttt atttatcata gttactctaa gtatctatca
      841 tttatctagt ataaaaaatt atctaaaaaa ttcacgacca cttaagttaa atctttgtat
      901 attaaatatc tgtctattct taagcatgat ccttctgtta atagcttcta caaaagtaat
      961 aaatatccat aatgaagccg aaaataattc tcttaaatat tgggaaagaa ctactaattt
     1021 atataaaaca aatattacta atcaattaaa tagaaacaac accgtagaag aaaataacta
     1081 cttgaaaaaa gctagtaaat ttgtttataa aatacagaag aattataaaa cttttattat
     1141 cgcgccatac aattacgcaa ctatccaaga aaataataaa gaatttttta ttggacaaaa
     1201 agtttatcca aattttgaag actatgttag tcaacctgct ggtgcaggaa tatgtgttga
     1261 tttaaactat ctaaaatatt acaatcctat ttcttttgaa gaatctactg acttaaattt
     1321 aataaactct gatccactaa ctacttacat tctagtacct aaaaaatata aagaatatgc
     1381 aaaaatgatt gaaaaaaatt atttagaaga catacagttt agacttgaag actctcctcc
     1441 tcaagctcct tataaaactc ctaatttgaa aaatttaaaa ataaaactaa tatttgttaa
     1501 taataaccaa aagtatttta gttttaatac cttatatgga aaggcaaaag ataactatac
     1561 aataactgat cccatagtta gagttattaa tccagaaata acagaatcat tattttgggg
     1621 aaatatattg actagtgatg gtggattatt ttttgatcag aaacatactt caaaccaaaa
     1681 cttttttaat caaattaatc cgtatataaa agaatttaac ctagaaaata tcataaattt
     1741 ttcttattct gtttttcaag aaactggtga attattatct caaaaaaaag taaataaatt
     1801 aaatgcatat ctacaatatc ttttaatatt aggattattt ataataatta attttcaaat
     1861 gatttttgtt agttttacta taaattcaaa aaaaatagct ttaaaaaaaa tatttggata
     1921 ttcaccaata agtcaagttt tagatttatg tttactccct tttacaatag agttatttgc
     1981 tactatgtgt tttcaattga tattttcaaa aaacacttac ttgtatttta taatttttat
     2041 gatcttttta ataaatctaa ttacttctat tattatttat aaaaaaaata gttttaaata
     2101 ttttaaggag aatattttat gatattatca gttgagaatt tcaccctgtc aattaataaa
     2161 aaaaagatca aacaaaattg gaacttttca atatcagaaa aagattttat tatagtaaca
     2221 ggaaaaagtg gtagtggaaa aactactctg ttaaataatt taagcctaat agataaacaa
     2281 tataccggta acttattcta ttttgaaaag aaagtttcta aacatgatat acaacgtcta
     2341 cgaagaaatc atattagcta tttatttcaa aactatgctc ttcttgaaaa tcaaacagta
     2401 caatacaatt tcgaattagc aaaaaagttt aacagtaata tttccaatga tcatatttat
     2461 aatttattaa cagatttttc tttgccaaaa gatatcttaa aagaaaaaat ctttctctta
     2521 agcggtggag aacaacaaag aatagcatta attagaaata tgttaaaacc atttgatata
     2581 ttatttgcag atgaacctac aggaaattta gataaagaaa actcaacttt tataatgaac
     2641 tttctgaaaa atttaacaga aaaagaaaaa aaagccgttg tactagtaac acatgactta
     2701 aatgttctaa actatggaac taatattata gaattataat ttttacccgt gatgctagct
     2761 gatttaaaac tagcaccacg ggtatttatt ttatattcca tctctcctat cccaaaattt
     2821 aaataatcac ttaataaaaa ggaggcaccc aagtgatttt attgaaaaaa gagtatgaaa
     2881 aattttatga tagacttatt tattctttgc aattgccaaa aaaattaaac cgtcaattac
     2941 aaaagactta tgaaactaaa tcaactgaag aatgggagag ttattgctag atttttggta
     3001 gaagtaacaa aaagtggaca tattatcata atgactcgcc gaaagtctga taaaaaattg
     3061 gctaaaaata ttgctgaatg tttaataaaa ctatcagttt tagctaaata gttagtagtt
     3121 tacacaaaaa agcatttatg ttttttgttg caaagcaaca atttcatctt cggtgaaata
     3181 agcgaaactt tacaaaaagt ggctagcatt tgctagcttt ttttgtattg tggagcgtgg
     3241 cttttgaggg ggattcaagg gggagcttgc gcccctttga caagcggtta tttgcaccaa
     3301 atacggaggt atttggctag caaatataga gcttgccaaa cccttttagt gagctttact
     3361 cattgtacct ttttaggtac aatggtattg cgaacgatac gtccgtagga cgtactgaaa
     3421 gggtgggact tatgagcgaa caaaatcaaa atttggctag caattcttct aaaaaatata
     3481 cctatcgatc cgagccaaaa caaatcagtt ttcgagtgag cgaatccgaa tttgcaaagc
     3541 tgaagcaatc ggctgaagct ttgcaaatga gtgtgccggc tttcgtgaaa gccaaggcac
     3601 aaaacgcgcg tctggtgaca ccgaaagtgg cgccagacat tgcccaggca atcgcgcgcg
     3661 atttagccaa agctggaggg aatatcaatc aaattgccaa gtggtgcaat acacaccaac
     3721 atgacgttgc tcctggagac gcccaacgtt tatccgaaaa cctaaaaatc atgcaaaagg
     3781 agctacaaaa gatatggcaa cagttaaagt aagtcgaacc acttcctgta gccgagcaat
     3841 caattacgca gaaccacgag caaccattaa aacaggaata aactgtgacg tcgattatgc
     3901 caaaagtgaa atgaaacaaa ttcgcatgct gtatggaaaa gacgaccgtg tacaggcaca
     3961 cttattgatt caatcttttc gaccaggaga aattaccgct gaaaaagcta atcaactagg
     4021 ggcagagtat gcaaaaaaaa tagcccccga tcaccaaaca gctatttaca cacatacaga
     4081 caaagaccat attcataatc acattgtcat taactccgtt aatctggaga ctggaaaaaa
     4141 atttcaagct catggtcaag catttctaaa caaatgttat gacctaaatg acgagatttg
     4201 tcttgcacat aacttaccaa tcactgagcg aggaaagaaa ccagaaaaac gaaccatgtc
     4261 tgaaataaaa ttaaaagaaa aaaatgaacc tgtttggaaa gacgaaattc ggattgcgat
     4321 cgatcaaaca atgaaaaaca aaaaaaccag aacctatgac caattttgta attccctcaa
     4381 aacattgggg attcattgct tcaatcgcgg gaaaaatttc acttacgaat tgatccacaa
     4441 aaagaaaaaa gtacgatcca acagactagg aaaggactac gaaaaggaga cgattttaca
     4501 tgagctggac agacgagaac gaacctataa ccaacagcga accaacgagc tacgaagagc
     4561 tcgagctagc attcgcgaaa ctgcagaaga agtacgaaga gaacaacgaa ctcttacgcc
     4621 tgaacaaccg gaaattagac cggctcgaag aaaccatgaa caacacgttg aaagaggaac
     4681 agaccaccaa caagaattat cgctctaaaa cgttaaaaga catagaaaac gaaataaaga
     4741 aacaacgtgc ttttctcgtt ccttatacgc aagaaaggat acaaactacg attgatttac
     4801 aagttaaaaa actccatcaa gagctattag accagcgaaa taaggaacag aaaaaggtgc
     4861 ttattcaacg atcattggat tttcttgccc ccttatgttg cttagtcctg acactgttcg
     4921 cattttggtt agtatgggga ggctaaaatg gcattttatt taccctatct actcattttt
     4981 gttagtattt caggaagtat ttggctcatc tataaaattt ttcaaacaag atattcatta
     5041 aaaggctcta aaattcgatt taagcgattt ttctttcttg gctgtatatt ttccttgata
     5101 atcgtttcaa gcggcttatt cggcgtttta gagggaaata agcgcgtttc tagaagtatt
     5161 ttattaggaa atgtcactca aaagtatgaa tcagcgcgtg ataaaaagaa aaaagagcaa
     5221 gcattagcac aaaaaatgga agagtttacc acttgttatg aggaaatgaa cgatattttt
     5281 gtgaatcaag aaaaacgttt gacggataaa aacatggaaa aactcactcg tctttatcaa
     5341 aacttgccag aaaaacagca aaaagaggtt caagacaact atgagcgaac caaaaaagac
     5401 gtgcaatatg taaaagatac aaagatagaa gaagcctgta gtgatctatt tggagacaca
     5461 aacccttggt ttgccagtga agaagaaaaa aaagaaaaac aacaatctgt cacatatgaa
     5521 cgctatgaaa tcttacttca acaagcaact aacatgcaaa atccgacaaa aaaagaaacc
     5581 gccttaaact atctaaaaag tgtcaaggaa tggttagatc agcaacaaca aaattgaatt
     5641 tgatttaaaa tcatgttaaa ctgaatgtgc aaatgatcaa acattagata cacgaaagcc
     5701 ccagagaggt cggattctct ggggcttttt ttcaggttga tgtcgccttt attgatttta
     5761 cttcttgttt cggttattca accaataact gaataaagag acacaaactc ctacaagaag
     5821 ggggcaaatg aacagttcaa acattagata cacctccttc cagtcgcaag actgtcggat
     5881 aggcgacaag ttattatatc ttaaaaaatt aaatttgtgt ggtgttttta agtatcactt
     5941 gttttagggt tgttagggct tgccctgacc gcctgtaagg cgcttctcga ctagattaag
     6001 ggaaattaat caagaattgt cttagaacgg cttagacaag ctttcagagt ctcacacctc
     6061 ctctttgaac aacggtttta gcaaaggagc cggaggcgac tgcgcagtat aatatgagag
     6121 catagcgatc acaccttttt agcgaccgta gggagcaagt tttccagttt agaaaaattc
     6181 gcgaaccttt ttggaggaaa aagtaggagt tttttaggcg ggaaattcaa tgtgctctta
     6241 agatatactg ttagatatac tgttagatat actgttaata ataactgtta gggattagca
     6301 accttatggg agagtaagta ttgacatagg tagtggggga taaattgtca cactatgggg
     6361 gataaattgt cacactatgg gggataaatt gtcacactat gggggataaa ttgtcacact
     6421 atgggggata aattgtgttt tacatcctct tatgtctatg ataacataat ttattgaggt
     6481 gatttttatt gagtaacgat atagttcgat ataataatgg attaaattct gtccctttaa
     6541 gaaaatttac ccctatagat atggatttat tttggggaat atgttctaaa atgaaaagaa
     6601 aaggtacaaa agaagaaatt tttacattta aacaaataaa agaattaatt gattacaata
     6661 taaatgcacg tggcgaccgt tttgctaatg atttaataaa aatgtcaaaa aaaatcatta
     6721 ctttgtcttt tatttatgaa gacgaaaaaa catatgaaca attaaatctg tttcaaaaat
     6781 ttaaaatcga taaagtgaat gaaacattat taataaaggt ttctgaagaa tttgaatata
     6841 tacttaattc aatcggaact aattttactc gtttcgaatt agaaaatatg acaaacttaa
     6901 agagtagcta cgttaaagag ttatatcgcc aacttatggc tcataaagac agaagatcta
     6961 aaaaaggtca ctggtttgta aaaatagaag attttagagc tctattagca ataccagaaa
     7021 gttatcgcat gagagatatt gataaacaaa tttttgccca agcgaaaaaa gaatttttaa
     7081 acgttaattc aactcaccct gctattttta aaacttttga aatcaaaaaa attaaagcct
     7141 acaaaggaaa taagatagga tctctgcaaa tttattttga agaaaatata ccttcaatta
     7201 ccttagataa ttggttagag aaataatacc taacaattta ggaggcaatt atcatgcaac
     7261 ccaaagaaac ctttacaata aaggaattat ctgaattatt tgaaataagt aggcaagcaa
     7321 tctctaaaca tgttgataaa ttagacccgt ccttattagc taaaaatgag cgtggttata
     7381 aggttgtatt acgttctgga gtattgcaac ttgctagcaa cttaggtaac caaaggttac
     7441 tatataggtt gcatgagtat gaacaagaaa acgataaaaa cgtttatata tcaagcaaaa
     7501 acgtgattgt aaacgagttg ctagataagt tgcgtgaaga aaatgaattt ttacgagaac
     7561 aactttccct caaagagcaa aataattcta atatgcaaaa attactcgac cagcaacaac
     7621 agctcaccct acaggcaaat caacagatcg aaaaactgca agaacagtta caattagctt
     7681 atacggaaga aactccagaa gatgaatcaa ctactattct ttctgaagaa acagaatcga
     7741 tagaaaaaca cacaaaaaag aagaagcaaa aatggtggca actttggaaa taactggagg
     7801 gaacattcat gacaagaaaa aatcttctat tcggttctat tttactagga attctaggct
     7861 tcagtccgat gttattgtca gtaatcaata aagggaataa acatggtgaa ttgaaattga
     7921 atccaaatta ttacaacagt cctaacgaag aattgtttgt aaaagagacg gataaataat
     7981 aaaaaagact gctcttccga gcagccagtt aaaaaataat gagtgtttat tcttgaaaaa
     8041 gcacgcgctt ataaagcgct accaaaattt tatcatattt tataaaaaat aacaatccag
     8101 taaaaaatga ctggtaaaaa ttcacaaaga aacggaaaga agcaactgca gaaaatcaag
     8161 ccttaaaaag agcttttgac ctagaccaat gaaaaatcac aaagtgagcc cctgtatccc
     8221 ttggtagaga ggctacttgg catagtgtac attatgtcaa ctatgcaatt aaataactaa
     8281 ctacttcata ttagcttaat aaaataatca aaatcactag gggaaaaaat attgtataaa
     8341 aaaatttttc attttttaaa taagaatagt aaatataaaa ataatttaag atatctatta
     8401 ctattaatcc agatagagca aaccaagtaa atattccgtg tgtaaagcta aaaaaattca
     8461 tttgaatcaa ttcaaataaa cctaatatag taacaaatat attaaaaata atagctgtca
     8521 ataccagtaa attattttgt ttatttttta aattatcttc atttaaaata gaatttatgt
     8581 tagtactata aaattcacaa atttttttta gtgtctctat atctggtgtt gttttgttat
     8641 tttcccactt tgaaatagtt tgacgtgtaa cattaaattt tcttgccaca tattcttgag
     8701 tatacccttt tttcactctt aattctctta gcttagatcc taaatcctcc attttttctc
     8761 ctatgtaaac gattatttac ataaaatttt atcataaaaa tactaacgcg ccaaaaaagt
     8821 tctttatttt ataaaacaca ctaaaataaa ataattctag taaaaaagat aggagaaata
     8881 agttatgaaa aaatttttat gtctttctgc tttaatgggt gtcttattaa ctagtggagg
     8941 aatagttagt gctacagaag ctttaaatct agatgtaccc gaagaccata ccgccattta
     9001 cggaggagga atggaagcta cagaagaagg attttcttct agtaaattga gatatgctgc
     9061 tggcggcgga gatttcaact gtggggttaa tggatttaaa gtttatgcaa attactacca
     9121 tgctagagct aaacatagtg caacagctaa aaatggacga ggcggtcaag tacgatcagt
     9181 tcagaaagct ggagtaagag cttatgcaac atgcaatgcc acacttactg gaaatactgg
     9241 ttggtggaat gtttattaaa aaagagagtt taaactctct tttttattta catttgaaag
     9301 gaaattcaaa atatgtt
//
LOCUS       DACEXQ010000096         9300 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.096, whole genome shotgun sequence.
ACCESSION   DACEXQ010000096 DACEXQ010000000
VERSION     DACEXQ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9300
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.096"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..1125
                     /locus_tag="IXH04_001600"
     CDS             <1..1125
                     /locus_tag="IXH04_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1852206.1"
                     /translation="KVLFFNNAINHGVLTPLGIEFSQTVGYSSLFLMEANPGPGLGIL
                     LSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRPYLFLAVMAGGTSGTLIFQW
                     LDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFVTFICAAILLKLPNKEKENK
                     TVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKLAAEKIDLSVGYSSIYELNN
                     QVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLSRIKEAIQFPKREEKKEIAD
                     ITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKKQFVISKESIEKIKIQEETI
                     YIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGEI"
     gene            1127..3160
                     /locus_tag="IXH04_001601"
     CDS             1127..3160
                     /locus_tag="IXH04_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ1852207.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            3171..3611
                     /locus_tag="IXH04_001602"
     CDS             3171..3611
                     /locus_tag="IXH04_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1852208.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(3811..4938)
                     /locus_tag="IXH04_001603"
     CDS             complement(3811..4938)
                     /locus_tag="IXH04_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1852209.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(4941..5618)
                     /locus_tag="IXH04_001604"
     CDS             complement(4941..5618)
                     /locus_tag="IXH04_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1852210.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            5882..7648
                     /locus_tag="IXH04_001605"
     CDS             5882..7648
                     /locus_tag="IXH04_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAQ1852211.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            7663..8100
                     /locus_tag="IXH04_001606"
     CDS             7663..8100
                     /locus_tag="IXH04_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1852212.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            8113..9264
                     /locus_tag="IXH04_001607"
     CDS             8113..9264
                     /locus_tag="IXH04_001607"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAQ1852213.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
BASE COUNT         3165 a         1639 c         1882 g         2614 t
ORIGIN      
        1 aaagtactat ttttcaataa tgcaatcaat catggtgtat tgacaccttt aggaatcgaa
       61 ttctcacaaa cagtcgggta ctcttcctta tttttgatgg aggcaaatcc tggacctggt
      121 ttagggatct tactttctat cctttgtttt gcagaaaaac aggaaaaagt aaatgcgagt
      181 ggagcattga tgatccatgc gattggcggg attcacgaaa tctattttcc atttgtcttg
      241 ctgcgaccgt atctattttt agctgtaatg gctggtggga caagcggcac gttgatattt
      301 caatggttag atacgggatt agctgcacct gtatcgcctg ggtcactact cgtgattttt
      361 gccaatgcgc cttatccaca actagctggg gtaagtatag ggatagcaat cagtacattt
      421 gtgaccttca tttgtgcagc gatactattg aaattaccaa ataaagaaaa agagaacaaa
      481 acggtcaaag aaaggggaga aatcttggaa ccatctatca ttcattccgt catttttgct
      541 tgcgactcag gtatgggatc aagtgctatg ggagccgccc tgctgaaaaa gaaattagca
      601 gcagaaaaaa tcgatctttc tgtcggatac agctctatct atgaattgaa taaccaagta
      661 aatcagctgg ttatcgtgca acatgaacta gcgggtcttg caagagaaaa agtacctcaa
      721 agtcaaatct atgtactgga tcactttttg gaaacagata tcttgctgtc acgaataaaa
      781 gaagcgatcc aatttccaaa gcgggaagaa aaaaaagaga tagcagatat cacagaacaa
      841 aaaacgtcgc ctgctaatat tgtatttttg tataaaaaca acagacgcgg ctcacaaact
      901 atgggcatga cgattttaag tcaattagca aaaaaagcga aaaaacagtt tgttatctca
      961 aaagaatcaa tagaaaaaat caaaatccaa gaagaaacga tttatatcgc acaaagagaa
     1021 ctagctgtaa ctgaacagct agaagaaaag atccctcggc tagttctagt tgaacattgg
     1081 gtagctacga atgaatacga aaaactcgtg aagggagaaa tataagatgt atttatcgcc
     1141 cagagaaaaa caattattag cagaatttct cagtagcccg tcacctgtat ctatccaaaa
     1201 aatgatgaat ttattgaagg tcagtaaacg gactatttac cgcgaattag ataatcttga
     1261 agtctcattg aaatcggtaa atgcttcttt gaaaaaagca gctagaggct catttgtcat
     1321 tgaggcatca gaagaagtat tagagcaatt aaaagaaaat acagatggga aaagtaatga
     1381 cttatccact gctaaacgac aacacgcgat tttagccgag ttgttactga cgacagagcc
     1441 gttgagtctt gctcatttca tggaatcata tcgcatcagt aataccactt tttatgcaga
     1501 tatcaaacag ttggaagaaa gtattcgcca actgcctttg gaaatcattc ggaaccaggg
     1561 atacgagatc gaaggaccgg aaaaataccg ccggcttctg acagcgaatg tattagaatt
     1621 agaaatcaat gaatatgaac tttttcatag tatttctttc gatgcatcac tgaattattt
     1681 ttttcgattc gttgatccgc aacatctatc tcttgcacgc aaagtaatcg gggaagaatt
     1741 gatccaaaaa aagaccaatc taagtgatag aaagcttgaa catttggttt tgatgctgac
     1801 attgacgatg gagcgtgtga cgaaaaatca tttactgaca aatgagacat ataatagtct
     1861 ggcaaacaaa gaattactac agacagctaa acgattgttt tcacggatcg cttctgagac
     1921 aaaacaactt tatccagtaa atgagatcgt cttttttgca ggattattaa atgacttcac
     1981 gaactcattt gatcagaatt tctttgaaga gacgttcgat acacagcttg cttatttagt
     2041 aaaacaactg attcaagaag taagtgaaga gacgaatgtc catttttatg aagatgaaac
     2101 tttgtacaaa atgctgttga ctcatttatc tggtgtgttt tcaagagccg ttttacaaga
     2161 agagacactg accaatccga ttttagaaaa aatcatggtt caatacgagg aagttgcgaa
     2221 tgcaatccgt aaagcagtag agcggatttt tcaagaaaaa aaattatccg aagaagagat
     2281 cgcttatatg gtccttcatt ttgcgaattc attggaacga agtccgaaag aaagcaatat
     2341 cagtattgca ggattttcgc caagtggatt agcttctact agcatgttgg aactacgatt
     2401 gaaaaggtat tttcccttca tccaacaaat tcatttctat cggattgctg atttaaaaaa
     2461 aatcgattta caagaagaat atgatgttgt tatttctaca tctattttac caggatacgg
     2521 tggaaaatat attttagtct ccccgctact gctagaagat gaagtcaagc agctaaaaga
     2581 agctttttcg catattgaga agaaaaaaag gcaaaaaatt acgtataaca gcaataacga
     2641 gtggatgata gaagatactt atgaggaaac aatgcatttc atggatcaaa tcaatcaatt
     2701 actcggtcgt ttttttatcc aagaactgga taatcctgag acgatttccg aaacgattcg
     2761 cagcttattt ccttattttc ctgaaggact agtaacggat gaagaggtgg ttcatcgccg
     2821 gctgcttaag cgatatgaac aagcacctat cggtattcct catacaaata tggggttatt
     2881 ccatacatcc agctcattga tttctaaacc gatcttttgt attttcaatt tgaaacatcc
     2941 gttgaccatt gaagggatgg atagacagcc agtcgatttg tcccgtatgt tagttatgct
     3001 ggcgccttcg ccaatccaag aaaaagacgg gattctttta ggaaagatca gtggagcaat
     3061 catcatgaat gacttgaaca ccgagatctt ccgctcagga aataaagaaa tcgtttatca
     3121 gttgttagcg aaaatcctga ttgaagaaat aaagaaatga aggtgaaaag atgatacagc
     3181 taccagcaga aaaaatattg ctcgatcaat cctatcaaaa ttggaaagaa gcgttgaccg
     3241 agatcggcca gtggatgacc caacaagggg gaatcaaacc ggcatatatc caatcaatga
     3301 tcgaaagaca agaaaaagcg agtgtctata tcgggaattt cgtagccttg ccacatggga
     3361 ctgacaaaga tgaagaagta gtagaagaag gaatctattt agtccaagtg cctgatggtg
     3421 tggactttgg aacaaaagag gaaccaaaaa tcgcgacact cttatttgta gtggctttaa
     3481 aaaaagaaca tcagttagcg gtgctccaag atttagcctt tttctgttca gacatcgacc
     3541 aagtaatggc gctcagtgat gcggaaacca ttgaagaagt ccaaatgatt tttgaacatg
     3601 gacatatata accaatgttc acagtaaaaa aagctgcctt gcatcaagta ataacttcaa
     3661 ccaagataaa aataagagga gctatttttg ttgggaggtt cttattactg cggatgcaag
     3721 cttttgaaaa ctatttattc tagaaaaccg tttatttgaa ataggggacg gtgacaaggt
     3781 ttttgtcacc gcccctatta tttttgtgtt ttaatagctc ttttttatat agatagtgcc
     3841 catacctatg aataataaaa cgatactcca tataccaagt gtcaagatac cattcatgaa
     3901 agtaacctgt tctgaagcta gtggcgagaa aatatctcct cctatgatca cattttgaaa
     3961 atcaacataa gaagttggca agtaatgact gattgattct atccatccaa tcgaagaaaa
     4021 caagcgatac tctgctaaga aaagacaaag aagtgtacct aaaagattga ctagagaatg
     4081 ttcaaataaa gcagatagaa gaaaactgat tgtacttaaa aagagcaccc acaacaacag
     4141 gaacagaaag ctctggaaaa gatactcata catcggcagg attcgtatct cctgaccaat
     4201 gatcgttcct acaggataac ggaagtctcc caaaccattt ttgattatta ctagtataga
     4261 gacgatcaaa aatggcatca cgagacttag catagctgca ccataataaa tggtaaaacg
     4321 gatgctatgt ttctttacca tactatccgg taatagattg actagagaga ttacctgctt
     4381 tcttttttca taagagatga tggaagaaag aaacaaggcg gttatacata aaatcaaaaa
     4441 actagaaatc attcctgaaa aaatattttc gtagtaattt gctaaagaga gatgcgctgg
     4501 caagtcaaaa atagaatatc tttggatctg atgttctttc atataacgga gcaactcgac
     4561 acgtttttgt tgttcaatga ttggaattcc ttgtctttga ccagaaatca gtcgatccag
     4621 tatatctttt tcataatgat aagttgcttc gaccatttgc tggacattcc ctgtgttttt
     4681 tgcatgtaag attgcttcga tcagtttgtt gcttttctct aaactttcca caaattcttt
     4741 ctcattctca tcttctttca acgtttcaat caaacgttgc atctcttcat agttatgatt
     4801 cagttcatcg aattctgtat aagcaagttt atgatcaaaa aaaagtggga atgcaaagag
     4861 cacgaccaac aatagaggaa ataaaaagtt tactttgttt tttagtaaga gattgcgttc
     4921 aaatgtaagt aaagacatag ttacacctca tataatttac gataaatagc taaaatctct
     4981 tccatatttg aggattctgt gacgatttct ccatttttta gaaaacatgc tttagaacaa
     5041 atcgattgga tattcgcgat agcgtgggaa gaaagaagaa tcgtcttccc ctctttctgc
     5101 cattgtctga tgaggctatt caagatatca atacttcctg ggtctaatcc gttcaacggt
     5161 tcatcgaata acatgacaga tgaatcactg atcaagtaag cagcaagcaa gacatgttgt
     5221 ttcattccta gagacatttt tccaatcggc aaagtaaggt actcagtcat cttcaatcgt
     5281 tggaaaacag tttggcattc tactttgctc tgccaatagt atttgatcaa agtcaaataa
     5341 tcaacagccg atagatagtc ggataactga ttcgttgatt ctagaaaaaa gaaagaccgg
     5401 cgataatctg tataagcagt aggagagatg ttgtgaaggg acaattgatc ataagttgtt
     5461 ggcagcagtt gggcaatgcc attcagcaaa gttgttttgc caaatccgtt cggggcaatc
     5521 aaagcagcta cttctcctga ttctactaaa agattcaatt gtcgaatcac acgtttttta
     5581 ccacgtttca tgttcatgtt tttgatcagt aatgtcatag ataggcctcc aatataaaat
     5641 ttaataacat aatagaatgg aaaacatcta aattaaatgc taacaaaagt tattgtttgc
     5701 atattataaa aaaattaaaa taacagtttt atttaaaatt gaagttttct gataccgaaa
     5761 tctttttatt ttgctgacat actcaaacct ttggcacaaa gtttcgtgcc aaagtacgac
     5821 tatttctctt tttagtaagc ggatacaatt agagtatcaa aaggagagag ggagagacaa
     5881 aatggaacgg acgattccca aaaacaacat ttcgctaaaa gcgagggtac aaaaattagg
     5941 cagtacttta tcaagcatgg tcatgccaaa tattggagca ctgattgcct ggggtgtatt
     6001 aacggctcta tttatcccag atggttattt accaaatgag gctttcgcta ccatggtaag
     6061 cccaatgcta acttatctga ttcctttatt gatcggttat actggcggaa aagtaattgc
     6121 tggtgaccgc ggatcagttg ttggggcaat tgcaacgatg ggtgtgattg tcggaacaga
     6181 tattccaatg atgctaggcg cgatgatcat gggacctctt ggtggatatg ccatcaagaa
     6241 atttgatcag ctattccaaa aacggatcaa atccggtttt gaaatgctag tcaataattt
     6301 ttcagcaggt cttatcggtt ttggattagc gttattaggt ttttctgcga ttggtccagt
     6361 tgtcgatgca ttgactcaag caatggcaaa aggtgtagag atcattttat ctgcccattt
     6421 gattccattg acaagtattt ttattgaacc agcaaaaatc ttatttttga ataatgcaat
     6481 caatcatggg attttgacac cattgggaac tgaacaagta gcagaggctg gaaaatctat
     6541 tctattcttg cttgaagcaa atcctggtcc aggacttggc gtattacttg cttttacttt
     6601 gtttggcaaa ggagcagcaa aatcttcagc accaggagcc atgatcattc actttttagg
     6661 cgggatccat gaaatctatt ttccttacgt tatgatgaaa ccgttattat tcttatcagt
     6721 tataggcgga ggaatgtcag gaagtttcat tttccaattg atggatgctg gactaagagc
     6781 gccagcttct ccagggtcga tcattgcgat tctagcgatg acaccgttca attcttacct
     6841 tcctgttatt ttgggcgtga ttgttgccgc agctgtttcg tttggtattt ctgcagtaat
     6901 tttgaaagcg gatgcgaaag atacaaatga agacttcgaa aaatctgttc aagagacaaa
     6961 ggcagctaag caagcttcca aaggtgccca aggtattcct caaggagcta cattctctgg
     7021 cgtacaaaaa atcatttttg cttgtgatgc aggaatggga tcaagtgcga tgggcgcttc
     7081 gatcttgaga aaaaaagtac aggaagcaga cttgcctcaa acggtgacca atcaagcaat
     7141 cagccaactg acagatgact cgaatacatt gatcgttact caggtcgaac tacaagaaag
     7201 agcaaaacaa aaagcaccga gcgcgcgttt tgtctcagta gaaaactttt tgaattcacc
     7261 tcgatatgat gaaatcattc aagaattaag cagcaagcag aaaacatcag tttctgaaat
     7321 tgagaatgag ccagcagaag agtttctttc agcaaacatc aaacaaatca aagagattct
     7381 acttgtccac gatgatcgtt ccggatcagc aacaatgggg atgaccgtgc tcaaagatat
     7441 attagacaag aaccatttaa ctatcccgtt aagaaaagtc aatatccaag atttaaaaga
     7501 tgaacctgat tcactgatcg tagccaaaga aagtttagcg gaaaaagtac agatggctgc
     7561 acctcaatcc attcatttat cagtcaacag catggtcaat ccgcaaaaat acgaatcgat
     7621 tgcacaatat ttgaaacaga cagcttaagg gaggaaaaca gcatgcaaag tttgccattc
     7681 gacatgatcg agctcaatcg atcttttaaa accaaagaag aagcgatccg ctattgcggt
     7741 cagatgcttg tagaagcagg gtgcgttgat gaggaatata tcgatgccat ggtagataga
     7801 gacaaaatgt tatctgttta tatggggaat tttatcgcga ttcctcatgg aacagatgaa
     7861 gcaaaaaaat atgtgaaaaa atccggcatc tgtgtcgttc aagtaccaga tggtgtagat
     7921 tttggtaccg aagaagaaga aaagattgcg actgttttgt tcggtattgc aggagtcgga
     7981 gatgagcatt tgcaacttgt acagcaaatc gcactttatt gcagtgatat ggacaatgtt
     8041 gtccagctag cagacgcgct cagcaaagaa gaagtaacgg aaaatctagc aattgcttaa
     8101 ggagagagaa aaatgagagc agtacatttt ggtgcaggaa atatcggtcg aggatttatt
     8161 ggagaggttt tggcagctaa tggatttgag attactttcg tagatgtgaa tcacacgatc
     8221 attgatgctt taaatgaaag aaaagaatac gaaatcgaat tagcagatga atcaaaagaa
     8281 aaaatcactg taaaaaatgt caaaggaatc aacaatcaaa caaatccaga aaaagtaatc
     8341 caagaaatcg ctgaagcaga tttagttact accgccattg gcccgaatat cttacctttt
     8401 attgctgagt tgattgcaaa aggtatccaa gaaagagaaa aggaaggaag cacaacatct
     8461 ttagatatca ttgcctgtga aaatatgatt ggcggttcag catttctaga aaaagaagtg
     8521 tataaatatc ttccggaaac atcatttact gacaaatata tcggctttcc aaatgcagca
     8581 gtcgatcgaa tcgtcccatt acaacaccat gaagatcctt tattcgtcca agtggaacct
     8641 ttcaaagaat gggtcattga cgatagccaa agaaaaaata aaaaaatcca attaaaaggg
     8701 gttttatatg tagatgatct agaaccatat atcgaacgca aactatttag tgtcaatact
     8761 ggtcatgcga ctgtcgctta tacaggtgcc ttgttaggtt accagaccat tgatgaagca
     8821 atgcaagatg cgttagttgt ggcacaatta aaatcagttt tgcaggaaac aggaagtttg
     8881 ctgattgcca aatgggggtt tgatgcagag cagcatcatg cgtatattga aaaaatcatt
     8941 catcgcttcc aaaacaaaaa tatctctgat gcgatcacac gtgtcgctcg gacaccatta
     9001 cgtaagttag gttatcaaga aagatttaca cgtccagtga gagaactaca agaacataac
     9061 ttgacttgcc cacatttgac ggcaacaatg gggattattt ttaattacta tgatcctgaa
     9121 gatgaacaaa gccgtcaatt gcatgaaatg aagatccatg aaaacttgga acaattgatt
     9181 caagaagtca caggaatcaa tgatccgaaa acaatcggga atatcaagca aaacgtgaac
     9241 cgttatgcca agcaagtcgc ttaaaatagg atacgaaaaa ggggaatgtg acgaaatttt
//
LOCUS       DACEXQ010000097         9271 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.097, whole genome shotgun sequence.
ACCESSION   DACEXQ010000097 DACEXQ010000000
VERSION     DACEXQ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.097"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..1330
                     /locus_tag="IXH04_001608"
     CDS             <1..1330
                     /locus_tag="IXH04_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HAQ1852214.1"
                     /translation="SNRKPKNLWTENKEGNKITYYRADNERDETRFIVDRMQEEIRSN
                     HRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIKDILAYLNVLVNP
                     QDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLANIGGKAGQQLGA
                     FGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARIENLEEFLTVTQE
                     FDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLMTLHAAKGLEFPV
                     VFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNAFSRTLYGRTQYN
                     RPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVPSKSATGGENNSW
                     KPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAPIEKA"
     gene            1347..3383
                     /gene="ligA"
                     /locus_tag="IXH04_001609"
     CDS             1347..3383
                     /gene="ligA"
                     /locus_tag="IXH04_001609"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAQ1852215.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            3489..3794
                     /gene="gatC"
                     /locus_tag="IXH04_001610"
     CDS             3489..3794
                     /gene="gatC"
                     /locus_tag="IXH04_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAQ1852216.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            3794..5266
                     /gene="gatA"
                     /locus_tag="IXH04_001611"
     CDS             3794..5266
                     /gene="gatA"
                     /locus_tag="IXH04_001611"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAQ1852217.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            5263..6693
                     /gene="gatB"
                     /locus_tag="IXH04_001612"
     CDS             5263..6693
                     /gene="gatB"
                     /locus_tag="IXH04_001612"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAQ1852218.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            6703..7758
                     /locus_tag="IXH04_001613"
     CDS             6703..7758
                     /locus_tag="IXH04_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAQ1852219.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            7869..9242
                     /gene="rlmD"
                     /locus_tag="IXH04_001614"
     CDS             7869..9242
                     /gene="rlmD"
                     /locus_tag="IXH04_001614"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ1852220.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVKAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
BASE COUNT         3204 a         1673 c         2084 g         2310 t
ORIGIN      
        1 tagcaatcga aaaccaaaaa acttgtggac agaaaacaaa gaaggaaaca agatcactta
       61 ctaccgtgca gataatgaac gagacgaaac ccgttttatc gtagaccgga tgcaagaaga
      121 gattcgaagc aatcatcgga attacggcga ctttgccatc ttgtaccgga cgaacgcaca
      181 atctcgtgta atggaagaaa ctctgttgaa agcaaatatt ccttataaaa tggttggagg
      241 gcacaagttc tacgatcgta aagaaatcaa agatatttta gcgtatttga atgtgctggt
      301 aaatcctcaa gattcaatca gttttgagcg tatcgttaat tcgcccaaac gaggcatcgg
      361 accaggatct atcgaaaaac tacgcagttt tgcttctctt catgaatggc cactgcttga
      421 agcagcgcaa aatgtggatc tggcaaatat cggtggaaaa gcaggacagc aattaggtgc
      481 atttggagag atgatccaag aagtcactca gatgatccca tacttgacag tgacagaatt
      541 gacgaaagag gttttggatc gtagcggata tctagaagac ttaaaaatcc aaaatacatt
      601 agaagcacaa gcacgcatcg aaaacttgga ggaattttta acggtcacac aagaattcga
      661 caaacaattc gaacagcaaa atgaagaaga cgctgatgca ccagaagaaa aactaacggt
      721 cttcttaaat gatctggcac ttgtttccga cattgacaat ttagaagaag atgcttccca
      781 agtgacattg atgactttgc atgcagccaa aggattagaa tttccagttg tattcctgat
      841 tggtctagaa gagggggttt ttcctttatc tcgagcattg atggaagaaa gcgaactgga
      901 ggaagaacgc cgtctcgcat atgtggggat cacgcgagca gaagaagcgt tgtatttaac
      961 aaatgctttc tccagaacat tatatggacg gacgcaatat aaccgacctt ctcgttttgt
     1021 agaagaaatc gatcaggagt tactggaaat cgaaggcatg cgtcctacgc ctaaaaaaac
     1081 gcctgtattc gctaaaaaaa cagcatatag ctataaacag ccagagacag cggttgttcc
     1141 ctctaaaagt gctactggag gggaaaataa cagctggaaa cctggagata aagtcaaaca
     1201 caaaaaatgg ggtctaggaa cagtcgtccg agtaagcggg acgtcgaagg atctagaact
     1261 agatgtggct ttcccaagtc aaggagtaaa acgtcttttg gctgcctttg cgccaattga
     1321 aaaagcttag tagtggaggg taaatgatgg aacatgaaat gacgattgac gaagctgcta
     1381 aacgagcaga agaacttcgt acaagactga accaatggtc tcgcgaatac tacgtagaag
     1441 ataaaccaac agtggaagac tatgtttatg ataaagaata cgcagaatta gtagcaatcg
     1501 aggaacaata tcctgatttg atcacgtctg attctcccac tcaacgagta ggcggaaaag
     1561 tattagaagg ttttgaaaaa gtcactcatg atattccgct ttacagtttg aacgacgttt
     1621 tcagcaaaga agagctgata gcttttgatc aacgtgtaca aaaagcagtg ggacgtgtag
     1681 ttgactattg ctgtgaacta aaaatagatg gactttccgt ttctttgcgt tatgaagatg
     1741 gaaatttcgt gagaggtgcg actcgcgggg atggaactgt aggagaaaac atcactgaaa
     1801 acctaaaaac tgtccggtcg gtcccaatca aattaaaaga accaatgaac atcgaagtaa
     1861 gaggcgaatg tttcatgcca aaacggtcat ttgtccaatt gaaccaagac agagaagcag
     1921 agggaaaaga tatctttgcg aatcctcgga acgccgcagc tggttctttg cgtcagttag
     1981 attctaaaat cacagcgaaa agaaatctgg acactttttt atacacagta gctgatttcg
     2041 gtcctatgca agcaaaaacc cagtatgatg cgctagaaga gctagaaaaa attggattcc
     2101 acacgaatcg ggaaaaacgt ctatgtcatt cgattgatga agtgtggtca tacattgaag
     2161 aatatcatga caaacgtgtg gatttgccct atgaaatcga tggaatcgtc atcaaagtga
     2221 atgaattctc gctacaagat caacttggtt ttactatcaa agcaccgaga tgggcagcag
     2281 cttacaagtt tccgccagaa gaagttgaaa cgctaatcga aaacatcgaa tggacagtcg
     2341 gtcgaacagg ggtagtaacg ccaacagcta tcatgacacc agtccgagta gcaggaacga
     2401 cagtaagccg tgccagcctg cataacggtg attatattaa gttgaaggat atccgcttga
     2461 aagatacagt tctgatttat aaagcgggag acatcatccc agaagtttcg caagtcgtgt
     2521 tagataaacg acctaaagac agcgaagaat accaattgcc tacacattgt ccagtttgtg
     2581 gaagtgaact tgtccatttg gatgaagaag tagccttacg gtgcatcaat cctaaatgcc
     2641 ctgctcaaat gaaagaaggt ttgaaccact ttgtttcaag aaatgcgatg aatatcgatg
     2701 ggttggggcc gcgcgtacta gaacagatgt atgataaaaa actagtagca gatgttgctg
     2761 atctttacaa attgacagaa gaagagttat tgacgttgga caaaataaaa gaaaaatcag
     2821 caaataatat tttgacagca atcgacaaca gcaaagataa ttcggtcgag cggttgattt
     2881 tcgggttagg aatccgacat gtcggagcga aagcagcaaa aattttagca gaacatttcg
     2941 gtgatctaga aacattgagt aaaagtgact atgagtcgat tattgcgctt gatacgatcg
     3001 gtgatatcat tgccgacagt gtagtcactt atttcagtaa cgaagaagtc catgaattga
     3061 tgaatgaatt aaaacaagca ggcgtcaatt tcgaatacaa agggcttcga aatgcccaat
     3121 tgcaagaagt cgagtctcct tttaaagaaa aaacagtagt ccttactggt aaactaacac
     3181 gatttactcg tgaagaagca aaagagacga tcgaaaatct tggcgggaaa gtgactggta
     3241 gcgtatcgaa aaaaacggat atcgtggtag caggagaaga tgctggcagc aagctgacca
     3301 aagcacaaga attaggaatc gaagtttgga cagaagatca aatggcagat gcattggcta
     3361 aaagtcgctc agtagaagaa tgaaaaatga gttttttcag acaaatttca ataaacaatt
     3421 tcagacacgg cactcttgtg ttaaaataga gagcaaagtt tttaactcgt ttagaaagaa
     3481 ggggtagtat ggcgattagt gaagaacaag tgaaacacgt tgccaaatta gcaaaacttg
     3541 cgttcgcaga tgaagaactt gcaagtttta ccgaccaatt aggtaaaatc atcgatatgg
     3601 ttgaaatgct ggaagaagta gatacggaag gtgttccatt tacgtcgaat gtggttgaaa
     3661 cagtaaacgt gatgcgacaa gatcgggcgc aagctggatg gaaccgcgat gaattattga
     3721 aaaatgtgcc ggaaacagaa gacggcttta tcaaagtacc cgcaatcatc gataatgggg
     3781 aggctggcgc ataatgacag aattacacaa tcgttctcta gaagaactgc atcatatgct
     3841 tgtatcgaaa gaaatcactg tacaagattt gacaaaaaca acatttgaac gaatcaaaga
     3901 aacagagcct gaggtcgagg cattcattac attgaacgaa gaaaaagcat tggaacaggc
     3961 aaaagcttta gatgaaaaag ggattgatga aagcaacgtc cttgctggta ttccaattgg
     4021 tatcaaggat aatatcgtaa caaaagatat cttgacaacc gctgcatcta agatccttta
     4081 caattttgaa ccaatttatg atgcaacggt catggataaa atctatcaag cagatatgat
     4141 ccctgttggt aagctaaaca tggatgaatt tgctatgggt ggaagtacag aaacttctta
     4201 tttcaagaaa acaaaaaatg cttgggaccc aacaaaagta cctggtggct cttctggtgg
     4261 ttctgcagca gcagttgcag ctggacaaat tccagtttct ttaggtagtg atactggggg
     4321 aagtatccgc caaccagcag cttttaacgg catcgtaggg atgaaaccta cttatggccg
     4381 tgtctcacgt tttggtttga ttgcctttgc ttcatctctt gaccaaatcg gaccaatgac
     4441 ccgtacggtg aaagataatg cattagcatt gacagctatc agcggttatg acgaaaaaga
     4501 cggtacttct tcaggcgttt cagttcctga ttttgcagag ggactgacag gcgacatcaa
     4561 aggaatgaag attgctttac ctaaagaata cttaggcgaa ggtgtggccc ctggcgtaaa
     4621 agaagctgta ttgaaagcag cagaaacatt ccgttcatta ggtgctgttg tggaagaagt
     4681 cagcttgcct cattcaaaat acggtgtggc tgtttattat atcattgcct catcagaagc
     4741 aagttccaat ttgcaacgat tcgatggtat ccgctacggt tatcgctctg aaaatgtgaa
     4801 atcattagat gatgtatatg tcaactcacg ttcagaagga ttcggtgaag aagtaaaacg
     4861 ccgtatcatg ttaggtacat tctccttaag cgcgggttat tatgacgctt acttcaaaaa
     4921 agcaggacaa gtccgtacat tgatcaaacg agattttgaa aaagtctttg cagattatga
     4981 cttgatcatc gggccatctt caccaacagt tgcattcgga atcggtgaga acattaacga
     5041 tccaatcaca atgtacatga gtgacatctt aacgatccct gtcaacttgg ctggacttcc
     5101 aggtatgtct gtaccggcag gtttatcaga aggattacca gttggattgc agatcatcgg
     5161 taattatttt gatgaaaaaa cgatgtatca agcggcatat gcgtttgaac aagcaacaga
     5221 attccataaa caacaaccag ctattttagg agggaaagac gaatgaattt tgaaacagtc
     5281 attggactag aagtccacgt cgagttaaaa accaactcaa aaattttctc tccagcgcca
     5341 gctcattttg gtgcagaacc aaacagcaat acaaacgtga tcgactgggg ttatccaggc
     5401 gtgctgccag taatgaataa agctgcgctg gaattcggga tgaaagcagc tttggcatta
     5461 aactgtgaaa tctctaaaga tactcatttt gaccggaaaa attactttta cccagataat
     5521 ccaaaagcat atcaaatttc ccaatttgat caaccaatcg gtcatgacgg atggatcgaa
     5581 atcgaagtag aaggtaaaaa gaaaaaaatc cggatcgaac gtgtccattt agaagaagat
     5641 gctggaaaaa acatgcatgg tattggcggt tattcttacg ttgatttgaa ccgacaaggc
     5701 acgcctttga tcgaaatcgt ttctgaagct gatatgcgtt caccagaaga agcatacgct
     5761 tatctagaag cattgcgatc aattatccaa tttacagaag tcagcgatgt gaaaatggaa
     5821 gaaggctcta tgcgctgtga tgccaatatt tctcttcgcc cttatggaca agaagagttc
     5881 ggtacgaaag cagaattgaa aaacttgaac tctttatctt tcgtgaaaaa aggtttagct
     5941 tttgaagaaa aacgtcaagc aaaagttctt ctttcaggag gagaaatcca acaagaaaca
     6001 cgccgttttg acgaaacaac gaataaaaca gttttgatgc gtgtcaaaga agggtctagt
     6061 gattatcgtt acttcccaga gcctgatatc ccgaaattcg tgatcgatga cgaatggatt
     6121 caaaaagtaa aagctagttt gcctgagatg ccagcttctc gtcgcgaacg ctatatccgt
     6181 gagtttggac ttccagagta tgacgcaatg gtattgacac ttacaaaaga aatgtctgat
     6241 ttttttgaag ctgttttgga aaatggtgca gatgccaagc aagcatctaa ctggttgatg
     6301 ggtgaagttt ctgcttactt gaacagtgag aaactggaat tagctgagac aaaattgaca
     6361 cctgagaact tagcaggaat gattcgtttg atcgccgatg gaacgatcag ctcaaaaatc
     6421 gccaaaaaag tcttccgtga attgatcaca aatggcggag aagcccaaga agtagttgaa
     6481 aaaaatggtt gggtgcaatt atctgatccg aacaaactac tgccaatcat taatgaaatt
     6541 ttggataaca accaacaatc tgtcgatgat ttcaaaaatg gaaaagatcg tgcggttggc
     6601 ttcctagtcg gtcaaatcat gaaagcaact aaaggacaag caaatcctgg tgtcgtgaat
     6661 aagctactta aagatgaatt agcgaaacga tagaggctga aaatgaaaaa agcacgcgta
     6721 atttataacc ccacctcggg aaaagaatta ttgaaaaaaa atttggcgga tatcctacag
     6781 gcattagaag aagcaggatt tgaagccagc gcctatgcaa ctacaccaga accagattca
     6841 gccaagaatg aagcatgtag agtcgcagaa ctaggttttg atctagtagt ggcagctggt
     6901 ggcgatggca caatcaacga agtggttaat ggaatcgctc cgcttgaaca ccgtcctaaa
     6961 atggccatca tcccagctgg tacaacgaac gactatgctc gagcattaaa aattcctaga
     7021 gataatatta aagcagcagc agaagtgata aaaaaacacc aaaccgtaaa aatggacatc
     7081 ggcctttctg ataacagtta cttcatcaat atcgctgcag gtggctattt gacagaattg
     7141 acttatgaag ttccatcaga actaaagagt atttttggtt atcttgctta tcttgcgaaa
     7201 ggagcagaga tgctccctcg agtaaagcca atcaaaatgc gcttgaagta tgatgaaggg
     7261 gaatatatcg gaaatgcttc gatgtttttc ctagggctga cgaactctgt tggaggattc
     7321 gaacagatcg cgccagatgc caaattggat gatgggaagt tttctttaat cattgtgaag
     7381 acagcaaata tctttgagat cttgcatatt gcagcactca tgctgaatgg cggtcgacat
     7441 gtcgatgatc caagaataat ctataccaaa acaagcagtt tgttcgttga aacagaagat
     7501 gatatcaatc gaccagtaat gatcaactta gatggtgaat acggcgggga agcaccgatg
     7561 catttccaaa atcttcatca acacattgag ttctttgcca ataccgatca gattcctgat
     7621 gaagcaatca caggtacaga tgaagaagaa ttagaggttg tcagcaaaga attcgtaaaa
     7681 gaagtcgaac gtttaacgga tgaagatatc gacggtgacg gaaaaatagc agacgaaaca
     7741 cctaatcaaa agaattaatc aaaagccggt gctgaacgta gtaaggtcag tgccggtttt
     7801 agttgtgtaa ttagctgatt tatagtagag ttcagtagga ttgaaaaata ggcttaaagg
     7861 agcaaaatat gacagaacag caaatagtaa agaaaaacga acgatataca gtcgaaatcg
     7921 tcgacttgag ttatgaagga atgggggtag caaaaatcga tggttatcca ttatttatcg
     7981 aaaatgcact ccctggagaa gaagtagaaa tactagtagt aaaagcaggc aataagtttg
     8041 gatatggaaa agtcgaaaca tttattacac aatcacctga tcgtcagcct gtagaaaaca
     8101 atgcattact tcgcataggt atcgcacctt tagcccattt gaagtatgaa caacagcttg
     8161 tcttcaaaca aaaacaagta gagcatgtaa aggcgaagat tgctaagatg ccagaagttc
     8221 ctgtacttcc aacggtcgga atggcagaac cattcggcta ccgtaataaa gcacagatcc
     8281 ctgttcgccg tatcgatgga cagttagcca ccggttttta caaaaagaat agccatgatc
     8341 tagtccctat cgaagatttt tatatccaag atcctgtcat cgatcaagca atcaaagtag
     8401 tcagagatat cttgcaacgg ttccaagtac gaggatataa tgaagaaaaa aatgaaggac
     8461 agcttcgtca catcattatc cgtcgtggtc attattccca tgaaatgatg gtcgttttag
     8521 ttactcgtaa agaaaaattc tttaaatcca aggaaattgc tgaagctatt catgaagaat
     8581 tgccagaagt ggtttcggtc atccaaaata tcaatgaaga aaaaacaaat gtgattttag
     8641 gagacaaaga aaaagtttta tatggcagag actatatcga agatcagcta ttaggaaaaa
     8701 cctatcgaat ctcagcaaag tcattttatc aagtaaatac tcaacaagca gagtatctgt
     8761 accaaacagc catcgagttt gccgagttag ataaaaaaga cattgtgatc gacgcttatt
     8821 caggaatagg tacgattggc ttgtctttag cagacagagt ggataaggtg tatgggatgg
     8881 aagttattcc agaagcaatt gaagatgcac agttcaatgc tttaaataat aaaatcgaaa
     8941 atgttcatta tgaagtcgga aaagcagaaa aagttatgaa aaaatggcaa gaaaaaggga
     9001 tcaaaccatc tgtgatcgta gttgaccctc cacgaaaagg attagatcat cattttattg
     9061 aatcagcaat cgaaatgtca ccagaacgaa tcgtctatat ctcatgtaat ccggcaactt
     9121 ttgcaagaga tgctaagtta tttgcagaag caggttatgc cgtgaagaaa gttcagcctg
     9181 tagatctttt cccgcaaacc catcatatcg aattagtcgc ttcattcgtt aaaggagaat
     9241 aaaaatggac caaaaagaaa aaatgaaaga a
//
LOCUS       DACEXQ010000098         9194 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.098, whole genome shotgun sequence.
ACCESSION   DACEXQ010000098 DACEXQ010000000
VERSION     DACEXQ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.098"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..206
                     /gene="lepB"
                     /locus_tag="IXH04_001615"
     CDS             <1..206
                     /gene="lepB"
                     /locus_tag="IXH04_001615"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ1852221.1"
                     /translation="SGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKDSRMFGL
                     VDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(310..912)
                     /locus_tag="IXH04_001616"
     CDS             complement(310..912)
                     /locus_tag="IXH04_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ1852222.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            1018..1487
                     /locus_tag="IXH04_001617"
                     /pseudo
     CDS             1018..1487
                     /locus_tag="IXH04_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            1593..2177
                     /locus_tag="IXH04_001618"
     CDS             1593..2177
                     /locus_tag="IXH04_001618"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAQ1852223.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            2180..3253
                     /gene="prfA"
                     /locus_tag="IXH04_001619"
     CDS             2180..3253
                     /gene="prfA"
                     /locus_tag="IXH04_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAQ1852224.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            3246..4085
                     /gene="prmC"
                     /locus_tag="IXH04_001620"
     CDS             3246..4085
                     /gene="prmC"
                     /locus_tag="IXH04_001620"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAQ1852225.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            4078..4257
                     /locus_tag="IXH04_001621"
     CDS             4078..4257
                     /locus_tag="IXH04_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852226.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            4279..5304
                     /locus_tag="IXH04_001622"
     CDS             4279..5304
                     /locus_tag="IXH04_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAQ1852227.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            5479..6723
                     /locus_tag="IXH04_001623"
     CDS             5479..6723
                     /locus_tag="IXH04_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAQ1852228.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            6876..7823
                     /locus_tag="IXH04_001624"
     CDS             6876..7823
                     /locus_tag="IXH04_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1852229.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            7957..8586
                     /gene="upp"
                     /locus_tag="IXH04_001625"
     CDS             7957..8586
                     /gene="upp"
                     /locus_tag="IXH04_001625"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAQ1852230.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(8725..8967)
                     /locus_tag="IXH04_001626"
     CDS             complement(8725..8967)
                     /locus_tag="IXH04_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852231.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            9088..9160
                     /locus_tag="IXH04_001627"
     tRNA            9088..9160
                     /locus_tag="IXH04_001627"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9121..9123,aa:Thr,seq:cgt)
BASE COUNT         3117 a         1582 c         1983 g         2512 t
ORIGIN      
        1 gctcaggatt ccaagcacaa gcagaaagtt catccacctt tacgagtgac tttgaatata
       61 ctgtgccaaa aggaaagtat cttgttctag gcgataaccg cttgatttca aaagacagcc
      121 gaatgtttgg gttagtcgac aaagatatga tccaaggaaa agttgttttc cgttattggc
      181 ctctttcaga aattaaaatc atgtgatgaa aaaataaaaa gaagctgtga catgtttttt
      241 gtcacagctt ctttttccga aaattccttc ttatttcttg gagttgctca ctttttttgc
      301 aatctcattt taagcttttt ggctgatgga taagaaaagc gaagcgtttt tgttcataaa
      361 tagcttttcc ccacgatgga aggtatgact agtagcagag acaaagcccg ttttttccaa
      421 tcgattattc aattcttctt ggctaaagcc attatggact ttcggatgac taatttgttc
      481 attcttgtca aagtctacga tgatcaattg accgccagga gctaaaattt cataaagttt
      541 tgtaagaata ttctccgtat caggaatgtg taaaagtaca agtgatagaa ggatgagatt
      601 agcttggaca gcaggaaatt cgacagaagc atcagcgtgg atcgttcgga catttttcag
      661 atctgctgct tggatctttt cttctgccat tttcagcata gtttctgaag catcggcaat
      721 gatcagttct ttgaatcttt ccgcaagcgg taaactaaca agcccagttc cgcctccgta
      781 atccagcagg actttttggt ggctgtcgcg cggtagataa gtagttagtt cttcgcgaat
      841 aatcttggct aatgcttgac ggtcagggga atcataatgg ttagcgatgg aatcaaaaat
      901 attttttgtc atcaaatagc tccttacgta aaactttgaa attttaactt attatagcac
      961 gtgaaaagaa agaaagcatg ctatactcaa ttcaaaaagg gagaatctag aaagaatatg
     1021 ccgatatcgt ttgtaaaaga tagagaggaa aaaggaaagt gtgtccgaga aatattacta
     1081 gacttgcctg agtggtttgg gcttcctgaa tcaacggaaa aatatatcga ggaatcaagt
     1141 aaactacctt tatggtgtga aaaaagaaaa gaagaatatt taggctttat cactctttca
     1201 cagacaagtg aagatactgc cgaaatctat tctattgtat gggaataaaa aaagactatc
     1261 atcaccaagg attaggaaca aaacttttta aagaagcaga aggttacgca gcaaaacatt
     1321 acaaatattt gcaagtgaag actgtagacg aggggcacta ctctatatat gatcaaacta
     1381 tttgtttcta cgaatcatta ggattttcaa ggttggaagt ttttccaaat ttatgggatg
     1441 aatggaaccc atgtttagtt cttgtcaaaa aattggaaca aaagtgagca atagatttaa
     1501 tactaatctg tgcttgatga aaattctcac tgtaaaaaat gctgaaaatc aagtataata
     1561 ccataggaac aaactggaag gtgaaaaaga gcatggcaca actatttttt aagtatggcg
     1621 caatgaatag cggcaagacg atcgagatcc taaaagtcgc acataattat gaagaacaaa
     1681 ataagccagt tgtcttaatg acaagcggta ttgatacaag agatggttta ggcgttgttt
     1741 caagccgaat cggactgaaa cgtaatgcga tgccgatatt tgaagaaaca gacgtatttg
     1801 acgtaattca gaaaatggaa agcaaaccct attgtgtgct gatcgatgaa gcgcaattct
     1861 taaaaaagaa acatgtcatt gcttttacga gaattgtgga cgaattggat attccggtta
     1921 tggcatttgg tttgaaaaat gattttcgaa acgagttatt tgaaggatca aaatatttat
     1981 tgctttatgc agacaaaatc gaagaaatga aaacaatctg ttggttctgc cacaaaaaag
     2041 caatcatgaa tttacattat attgatggaa accctgttta tgaaggcgat caagtgcaaa
     2101 ttggcggaaa tgaagcttat taccccgttt gtcgaaaaca ttactttcac ccatcaatga
     2161 ttacagaagg agaataagca tgtacgatca attacaagca attgaagacc gttatgaaga
     2221 gcttggagaa cttttaagtg acccagaagt gatttctgat acaaagcgtt ttatgcagct
     2281 ttcaaaagaa gaagcaaaca cgcgcgaaac agtcgaagtt tatcgtcgtt ataaagaagt
     2341 agtacaagga atcaccgata cagaagaact gcttggtgaa aaactggatg atgaaatggc
     2401 tgaactagca aaagaagaat tatctgaatt aaaaaaagaa aaagaagtct tagaagaaaa
     2461 aatcaagatc ttgcttttac caaaagatcc aaatgacgat aaaaatatca tcatggaaat
     2521 ccgtggagca gcaggcggag acgaagcagc attattcgct ggcgatcttt tcaatatgta
     2581 ccaaaagtat gctgaagcac aaggctggaa aacagaagtg atggaagcta acgttaccgg
     2641 aatcggtgga tataaagaag tgatcatgat gatcaccgga gacaacgtat attcgaaatt
     2701 aaaatatgaa agtggtgcgc accgcgtcca gcgtgttcct tcaactgaat cgcaagggcg
     2761 tatccatact tccaccgcaa cagttgtcgt gatgccagaa gctgaagaag tagagatcga
     2821 tattgaagac aaagatatcc gtacagatat ctatcatgct tcaggtgctg gcggacagca
     2881 cgtcaataaa acagcatctg ctgtacgttt gactcactta ccaacaggga tcgttgttgc
     2941 tatgcaagat gaacgttctc aaatcaagaa ccgtgaaaaa gcaatgaaaa tcttgcgtgc
     3001 tcgtgtgtac gataagatcc agcaagaagc acaaagcgag tatgatgcca atcgaaaatc
     3061 agcagtcgga actggggatc gttcagaacg gatccggacg tataatttcc cgcaaaatcg
     3121 tgtgactgat catcgtatcg gattaactat ccaaaaatta gatcagattt tagcagggaa
     3181 attagatgag atcgttgatg cattgatcat gtatgatcaa acatcaaaac tggaagctat
     3241 gcaaaatggg taaaatgacc tatcgagaag tcctgtcacg ggcttcttct tttttagagg
     3301 atcatgggaa agaagcatat agtatccaat ttttattttt ggaacgaaaa caatggaaaa
     3361 aacttgattg gctccttcat atgaacgaag agatttcaga agaagaacag cggttgatcg
     3421 aaaccgatct gcgacttctg cttgcagatc atccacctca gtatttgcta ggatatgcag
     3481 atttttatga tcatcggtta aaggtaactg aagcaacact gatccctcga ccagaaacag
     3541 aggagttagt cgaatggtgt ttagacgaga cgcctggtgt accgttagaa gtgatagata
     3601 ttgggacagg gactggagca attgctatta gtttgaaagc agcaagaaaa aattggcata
     3661 tatctgctgt ggatctttca gaagaagcat tagaagtagc gaaagaaaat gctcagaaag
     3721 aaggaacgaa aatcagtttt tatcatggag atacattagg accagtaatg gatcagcaat
     3781 ttgatgtgat tatttctaat ccgccgtaca tcagtcggaa tgaatgggaa ctgatggatg
     3841 aaagtgtccg gtcattcgaa cctaaaatgg ccctttttgc tgagaacgat ggattagcta
     3901 tttatgaaaa aatagcagtt gaagcaccaa gcgtactaaa atcaaatgga aagatttttt
     3961 tagaaatcgg atttcgccaa ggtgaagcag taaaaaaaat atttcagcaa gcatttccag
     4021 ataaaaaagt ggcgataaaa aaggatcttt ttggcaatga acgaatgatc caagtttatg
     4081 gttgaagttg tggatgaaaa attttttatt tgttttttta ttataaaaaa acacttattt
     4141 gacaaggatg taaacgactt tttggtttat caacaagttt atccactatt gacaaataga
     4201 ttatccacaa acagtggaaa acttgaggat aaactcagac aaaataagaa tgtatgaaaa
     4261 aggggttgtg gataaactgt ggaaacgaaa cgatatgatg aaacagaatt gcaagaggca
     4321 gccgaagcac tgaaagaagg agaacttgtt gcttttccta ccgaaacagt atacggattg
     4381 ggcgccaacg ctcttttacc aaacgctgtc aagaaagtgt tttctgtaaa agggagaccg
     4441 caagataatc cattaatcgt ccatgtcgcc tcttttgaac aagtaaaaga gtatgtggac
     4501 aactttcatc cggcagcaga gaagattgtg aagaacttct ggcctggacc attaactttg
     4561 atatttaaga tcaaaaaaga cactttgccc tcagttgtga cgggaggatt atccacagct
     4621 gcatttcgta taccggataa caaaaaaaca ctagaagtaa tcgaactttc cggtgttccg
     4681 cttgtcggtc caagtgcaaa tacgtcggga aaaccaagtc caacgactgc agatcatgta
     4741 tatcatgatc tacaaggaaa aattaccggg attattgatg atggagctac gcgaattggt
     4801 gtagaatcca ctgttcttga tttaagtgat ccaacagcca tgcctatgat tttacgacca
     4861 ggtgcagtaa cgaaagaaca aatcgaagca gtcatcgaat ctcctgttgc tattgatcag
     4921 catcttgtaa aagaaaatga aacaccaaaa gcccctggga tgaaatacaa acactactca
     4981 cccgatacac gtgttctgat ggtcagagat ggcgattggt caactgcagt tcaatgggca
     5041 aaaaataaga agatccgtgc aggtgtcata gcaagccccg aaattgcgga tcaagtgcgg
     5101 acagatactg cagctgttta tatgtataac gacaactcag tagaagcggc ggctaaagga
     5161 ttgtttgcag gtctaagagg attagatgaa ccaacacttg gattagattt gatttttgta
     5221 caagtgtatc cagaaactgg attggggaat gcttacatga atcgcttgaa aaaagcagca
     5281 ggacaaaatt attttgaaaa atgatcaatt ggttattgtc gcattctttt agtatgatac
     5341 aatgctatca agtaaatctt ctatcagtga acgaaaaact aagcgtaact aatcgtaata
     5401 gaagagatac gaatatttgg caatttgttt ttgatgggaa aaataaaatt gtaggaaaat
     5461 aaaaaggagc tgttgtttgt ggtagattac aaaacgtttg accctgatct ttgggctgcg
     5521 atcgccaaag aagaagaaag acaagaacat aatttggagc tgattgcctc tgaaaacttt
     5581 gtctccgaag cagtgatggc tgcacaagga agtatcttga caaataaata cgcagaaggt
     5641 tatccagggc atcgctatta tggtgggtgt gaatttgtcg atatcgttga aaatttagcg
     5701 attgaccgtg caaaagaatt attcggagcg aaatttgcga acgtccagcc tcactccggc
     5761 tctcaagcca acacggctgc ttatctcgct ttggtagaac caggggacac gatcttagga
     5821 atggatcttt ccgcaggcgg acatctaaca catggatcac cagtcaactt cagtggcaaa
     5881 acctatcact ttgttgctta cggtgttgat ccgacaacag aagttattga ttataatgtg
     5941 gtacgtattt tagctagaaa acatcaacca aaattgatcg tagcaggtgc cagtgcttac
     6001 ggacgaacga tcgactttgc gaaattcaga gaaatcgcag atgaagtagg cgcaaaactg
     6061 atggtagata tggcacatat cgcaggatta gtagcagcag gacttcatcc taatccagtt
     6121 ccttatgcag atatcacaac gactaccact cataaaacat tgcgtggccc tcgaggcggc
     6181 atgattttga caaacgatga agcattagca aaaaaaataa atagtgcggt atttcctggt
     6241 attcaaggcg gccctctaga gcatgtgatt gcgggtaaag cagtagcatt caaagaagca
     6301 ttagatcctg catttaaaga atatagtgaa caaatcatcg ccaatgcaaa agcaatggtc
     6361 aaagttttca atcaagcaat tggaaccaga gtcatttcag gggcgacaga taatcatctg
     6421 atgttgatcg atgttcgcga gttaggtata aatggaaaag aagcagagag cattttggac
     6481 agtgtgaata tcactgtaaa taaaaactcg attccttttg aaacactaag tccattcaaa
     6541 acaagcggta ttcgaatcgg cacgccagca atcactactc gcgggttcaa agaagaagat
     6601 gctgtaaaag tggctgaact agttgtcaaa gcattacaag caaaagatga taacgctcaa
     6661 ctagacgaag tcaaaacagg tgtacgcgaa ttaactgaaa aatttccgtt gcataaaaaa
     6721 taaaaattta aattccatta gtaaacagaa aagttcaatg ttgggctttt ctgtttattt
     6781 tttgggaaaa cggattcttt gacaatatcg gtgatgctct ctagtatatg aggtgtaatt
     6841 ttagtcttca cactaaatct cagggaggaa aagtcatgac tgatcgtatc tcgttagaaa
     6901 aggaagcagt agcattcagt gatgcaaatg cgcctcatcc gcgtatctat gaactagcac
     6961 cggaagaagg aagagtttta ctagaagaag tacaagaatc acctgtgaac aaatattccg
     7021 tcgatattga agacttgatg gtggatactg gcgaatgggg aaaactcaat attcgtctcg
     7081 tacgaccaga agggaatatg gataaattac cagttatttt ttatattcat ggagcaggct
     7141 gggtttttgg cagtgctcaa acccatgata aattgatcag agaacttgcg gttcgtacga
     7201 attccgtcgt tgtctttcca gaatatagtc gatcaccaga agcaaaatat ccaacagcaa
     7261 ttgaacaaaa ttatgctgtg ttgcaacagc tgaataaatt agccaagtca aaaaatctgg
     7321 atctcgagaa tctaatcgtt gcaggagatt cagtaggtgg taacatggct acagtcatga
     7381 cgatcttaac aaaagaacga aagggcctcc caatatccaa gcaattgctg tattatccag
     7441 tcacaaacgc tgattttgat acagattcct atcatgaatt cggagaaaac tattttttga
     7501 caagagaagg aatgatgtgg ttttgggacc aatatacgac gaatgaagaa gagcgtgctg
     7561 aaatcacagc ttcgcctttg agagcaaata taaatgaact aaatgatctt ccttcagcaa
     7621 tgattttaac gggagaagcg gatgtattaa gagatgaagg tgaggcttat gcgcgcaaac
     7681 ttcgtgatgc aggggttcct gttacacaag tacgcttcca aggaatcatc catgattttg
     7741 tcatggtaaa tgctatggat cagacaaagg caacgcgtgc agcaatggca ttatctactt
     7801 cttggatcaa gaaaaaagaa taagctttgc ttgtccgcag gaaaacttct tttcaaaaac
     7861 ggattttctt aggaaaaatg gcgagtttga ctagtcaatc acttgatttt gttttacaat
     7921 taaaaagaaa cgacagaaca aaggagagct taaaaaatgg gcaaatttca agtgatcgat
     7981 catccattga ttcaacacaa actaactatt attcgggaca aaaactgcgg aacaaaggtt
     8041 tttcgtgaag tagtcgatga aatcgctatg ttgatggcat atgaagtatc acgggatatg
     8101 ccattggaag acatcgtgat cgaaacaccg atcactgaaa caacacaaaa aacgttgtct
     8161 ggtaaaaaag tagcgattat tccaatcttg cgcgcaggta ttggaatggt tgacggtatc
     8221 ttggaattga ttccagctgc taaagtggga catatcggtc tttatcgaga tgaagaaaca
     8281 ttggaaccac atgaatattt cgtcaaactt ccagaagaca ttgatgcacg tcagttgttt
     8341 gtagtagatc caatgttggc aacaggcgga tcagcaatca tggcaatcga tgcattgaaa
     8401 gaacgtggcg cgtcgaacat taaatttgtt tgtttggtag cagcacctga gggagtaaaa
     8461 gctttgcaag aagcgcatcc tgatattgac atctatacag cttctctaga tgaaaaattg
     8521 aatgaacatg gttatatcgt tcctggacta ggggatgcgg gtgaccgttt gtttggtaca
     8581 aaataagtaa atacgaaaaa gggtgtgaca caacttttgt cacatccttt tttggtcatt
     8641 tttaacgttt gatgcagaac agctttttca cagctttcgt ttgcatataa aaaaaccatc
     8701 ccaaaaatgc agagatggtc aaattcaaat cttttttagt tctttgtagt taacgattac
     8761 tacaccctta ctttgcgtca tcaagttcgc attttctgca gtttcatcaa tctcaacaac
     8821 agcggaattt ttcaattgct ttgaaatcgt tccagtttgc gacttgccgt gaattgtgaa
     8881 cattacgtga gtattgatct tgaatttttc accaatttcc gatggtacga tagttgtatc
     8941 aaattttcca aaagttgcca ttttcataaa acctgccttt cacatcaatt ataccacgtt
     9001 ttatgaaaaa tatcatgaaa ttaaactaat tttccatcgg actctttaca atcagccaaa
     9061 tagagtatat aatttctttt ggataaggcc agcttagctc agttggtaga gcaacgcact
     9121 cgtaacgcgt aggtcgtagg ttcgatccct atagctggca ttaaaagaga agacagatat
     9181 atcaaggatt ttta
//
LOCUS       DACEXQ010000099         9184 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.099, whole genome shotgun sequence.
ACCESSION   DACEXQ010000099 DACEXQ010000000
VERSION     DACEXQ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9184
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.099"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(62..730)
                     /locus_tag="IXH04_001628"
     CDS             complement(62..730)
                     /locus_tag="IXH04_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1852232.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            864..1151
                     /locus_tag="IXH04_001629"
     CDS             864..1151
                     /locus_tag="IXH04_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852233.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1567..3105
                     /locus_tag="IXH04_001630"
     CDS             1567..3105
                     /locus_tag="IXH04_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAQ1852234.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3102..4067
                     /locus_tag="IXH04_001631"
     CDS             3102..4067
                     /locus_tag="IXH04_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1852235.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4054..5376
                     /locus_tag="IXH04_001632"
     CDS             4054..5376
                     /locus_tag="IXH04_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1852236.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5439..5543
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5617..6360
                     /locus_tag="IXH04_001633"
     CDS             5617..6360
                     /locus_tag="IXH04_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1852237.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6375..7367
                     /locus_tag="IXH04_001634"
     CDS             6375..7367
                     /locus_tag="IXH04_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1852238.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7379..8116
                     /locus_tag="IXH04_001635"
     CDS             7379..8116
                     /locus_tag="IXH04_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1852239.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8106..8828
                     /locus_tag="IXH04_001636"
     CDS             8106..8828
                     /locus_tag="IXH04_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ1852240.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8912..>9184)
                     /locus_tag="IXH04_001637"
     CDS             complement(8912..>9184)
                     /locus_tag="IXH04_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAQ1852241.1"
                     /translation="AETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIG
                     RYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3034 a         1577 c         1927 g         2646 t
ORIGIN      
        1 tatcatttta gtttacataa tgatatttct gtaaattaaa taaagtagtg aaaaataaga
       61 atcagtgctc taactgttca agaggatcaa gtggttcaaa tgtttctgta gtctgttttg
      121 cttccaattg gggcaaaaca gatccaattt ccagatactt cactttattc aatgtataag
      181 atagaataga acagctaagt aatgtataca gaaaatgtgt gagatccaaa taaagcaagg
      241 agacaactaa aataccaaaa gtaatcaaga aaagactttt gccaatctta atatgaaaat
      301 atttttcaat ggcttgataa atgatatctg ttccgcctgt gcttccgccg aatcgaaaga
      361 cgagaccgca accaatgcca gaaagaatac ctgtcatcaa actattgatc agtagactat
      421 ctacagcaaa atgtatatga aggctctccc ataaatgtaa aaagaaaact agcgcaaatg
      481 ttccgacgat cgtccggaga aaagcgtgtt ttccaaaaag aaacaatgag ataagcaata
      541 aaggtaaatt aaaaagcagt gaagtgacag aaggagcgaa tccaaaaaga ttatgcaaaa
      601 gcaaagcaat accagtaacc ccaccatctg ccagcttaga tggaatattg attcctgtca
      661 cagctaatcc gtagattcct gcgcctaaaa tgattccgca caattctgta aataaattta
      721 attttttcat ttctagcctc ttttcttttg aaaattttca gcaacttctt tattatactg
      781 acttatactc ttacttcaag aggaaaatga aaaaaaatta tttttggctg tttcttttta
      841 tttatcaaat aaggagtgga aaaatgcgag cagcagactt attgacaatt cttacaaaat
      901 cacccaaaaa aacagagtat caaggagttt ttttggctca agaacaaaaa ctggtgcctg
      961 tgacaatggt ccagataaat aaagcaaatc aatttatctt tttatttgaa cacggcaaag
     1021 caccacttgc tatgaaagac atctttcttt ttttgatgaa gaaccgtgag aaagctgttt
     1081 attatcagtc aaaagaagaa ctcattcctc tttatggagt gaaagagata gaaaataaag
     1141 tggtgatata agtaaaaata agaaaacaag acgtttatga tgtcaaaaca accatttttt
     1201 acatttttgg caaaaaagtg tcatgataca gtataatatt cgttgaacag gccaataaag
     1261 gtgttctccc ccacgagatt tctactttat tggctgtttc tttttattaa aataaaagtt
     1321 gtgtgtgtaa aaaagatata gctgattcca agtgacaatt tcgtatagat ttttatggta
     1381 ttcgtagtga aaaaacgaga accaaaaagt tgatccgact ttgttgcttg gattcttttt
     1441 ttgtcagcat atttagaggt atttttttct tttttttgtt cgtgctgtaa ggttaaggca
     1501 agacaagtta gttgggaaaa aggaaagaaa tgtttatact taaagaataa cagaaaggaa
     1561 ggtcgcatga ataattttat acaaacatta agtgaaagaa aaggcgagtt gctggaagct
     1621 gcatttcaac atctgtccat ttctctcatt tctttgctga ttgcagcact gattgctatt
     1681 cctttagcga tttgggcagc caatcataaa aaaatggcag aagtcttatt gcagatcacg
     1741 ggtgtgctcc aaacgattcc ttcacttgcc ttattaggtt tacttatccc atttgtaggg
     1801 atcggaactg ttccagcatt gattgcactt gttatttacg ctttgcttcc gattttccaa
     1861 aacacgtata ttggtctagc tgaaattgac ccgtctattg aagaagcagc ggtagcattt
     1921 ggtatgtcca gaatgagacg cttgctgaaa gtagagcttc ccatcgcatt acctgtcatt
     1981 atctcaggta tccgaactgc cttagtttta atcatcggta cggctacttt ggcagcattg
     2041 atcggagcag gtggattagg gacatttatt ttgttaggta ttgaccgaaa tacacctgtt
     2101 ttgacattga ttggtgcgat cagttctgcc ttgcttgcaa ttatattcag cggactgatt
     2161 cgctggttgc agcataagaa acctcgatat tccatcatta ctttagcagc agtggtgtgt
     2221 ttatttggcg gcgtttcttt atatcagtct gccttgttta aaaatgaaac catcacgatt
     2281 gctggaaaat taggagcgga acctgaaatc ctgatccaaa tgtataaaga attgattgaa
     2341 gacaacacaa aaacagaagt tgaattaaaa ccgaattttg gcaagacgag ttttttgttc
     2401 aatgcactgg atcaaggtca aatcgatatc tatcctgagt tcacaggtac tgtcctagaa
     2461 agcttggtga aggtacctga agcaatgaaa agtgctgatt tgacaaaaga aaaaacatat
     2521 gacgaagcag aagaattatt gaataagcaa ttcgatatga cattattaaa accaatggct
     2581 taccaaaata cgtatgcctt ggcagtcaag agatcttttg cgaacgaaca tcaacttaaa
     2641 aagatttccg atttggcagc tatctctgat cagatcaaag cgggattcac actggaattt
     2701 attgatcgtt cagatggata taaaggaatc caagaagcat atggcttgaa tttttcaagt
     2761 gtacaaagca tggagccaag tttgcgttac caagcaatca ataatggaga tgtcaatatt
     2821 gtggatgcct attccacaga tagccagctc aaggagtacg atctagtgac tctagaagat
     2881 gatcgagggt tattcccagc ttatcaaggc gcaccgctga tgaaaaaaga ttttgctaaa
     2941 gaacatcctg aaatcgttaa agcactgaat aaattaagcg gaaaaataac agaggatcaa
     3001 atgattgata tgaattacca agtcaatgtc gaaaagaaaa agccgacaga agtagcacga
     3061 atcttcctga aaaatgaagg attagtaaag gaggaagatc agtgactaaa ttaatcgagt
     3121 tcaaacatgt gcaaaaaaag tatgatggaa agtatgtcat cgatgaccta aatctggcaa
     3181 tcaaaaaagg agaaatcttt gttttggttg gtccttccgg tagtggaaaa acaactactt
     3241 tgaaaatgat caatggatta tctaaaccat cagcaggaga catctacttc aaaggaaaat
     3301 cgttaaatga atataatctg cagaaaatgc ggtggaatat gggatatgtg ctgcagcaaa
     3361 tcgctttgtt cccgaccatg acagtcaagc aaaatattga agtcatccct gaaatgttag
     3421 gttgggaaaa gcagaaaaga gcagaccgtg tagatgaact tcttcaaaaa gttggcttat
     3481 ctcctgatat ttatcgagat cgtatgccta gagagttgtc tggaggagaa cagcaacgga
     3541 tcgggatcat ccgagctatt gctgcaagcc cagatgtgat tttgatggac gagccattta
     3601 gcgcattaga tccgatttca agaaactcat tacaagaatt ggttttgtcc ttacacgaag
     3661 aattgggcac gaccatcgtt ttcgttacgc acaatatgga agaagcaatc aagctaggtg
     3721 atcgtattgc atttatgaag gatggagaaa ttatccaatg tgacacacca gagcagttat
     3781 tgatgaatcc taaaaacgac tacgttcggc atttctttga tgaaccgaaa cagacgaaag
     3841 agtggcgagt agaagattta gtcgtcaatg gatatttctt aaatgaaata ccagaaaatg
     3901 ctcgtcaaca agtttgcttt gatacgccta tgaaagaagt atattcgctt ctttcggtgt
     3961 atccaagcat tgtgatagta gaaaagcaaa gaagtattgg gtcgctgaca agtaaagaaa
     4021 tctttgcttt tttaagtcga gaggaggaag gaaatgaaaa catatgatgc aatcatcatc
     4081 ggttcaggtg tgagcggctt aagtgctgct tatggattga aagaagcagg taaaacagta
     4141 ctagtggtcg aagaagatct atggggcggg acttgtccta accgtggatg tgatcctaaa
     4201 aaagtcttgt taagtgcagt agaggcacgt aatcgggtaa aacaattgag tggaaaaggt
     4261 tttaacgaga tcccaactgc caactgggaa gaacttcaaa aattcaaacg aacatttacg
     4321 gatccagtcc cagaaagccg aaaaaaacaa ttagcagaag ctgaaatcga tcatctttca
     4381 ggaaccgctc gttttttgga tgattcatct attgaagtca acgaagaagt cttccatgcg
     4441 gattacttag ttttggcaac cgggcaaaga cctacgatct taccagtgga gggaaaggaa
     4501 tacttgaaaa ctagtgcaga ctttttatca ttgcctgtac tgcctaaaga aatcattttt
     4561 atcggtggtg gttatattgc ttttgagcta gctacgattg ccaatgctgc agggagcaaa
     4621 gtaaccattg tccatcataa tcaacggcca ttaaaagaat ttgaggcgtc tttggtagag
     4681 gaagccgtcc atcaaatgga agcatctggc attcagtttg cttttggtgt tgaaacccaa
     4741 aagatcattt cagaaggtac tcgctatcgt ttagtcggaa aagagacgga attagtagca
     4801 gatatgattt tttgtgcgac tgggagacag ccgaatacag aatctttagc acttgagcaa
     4861 gcgaatatcg tctttgataa acacggaatt gcagtcaatg attatctgca aacgagcaat
     4921 ccaaaaattt ttgcgtgtgg agatattgtt tcaaggaaaa ccccaaaatt gacaccagtc
     4981 gccacttttg aaggaaatta tgtagctaag cggataacgg atgcaacaag cgaacctatc
     5041 aaatatccga ttatacctac gatcgtctac gccagtccta aacttgctga agtaggagtt
     5101 acaaaaagtc acgcctcttc gtcagatcaa gtggtagaga tggatcttac cagttggttc
     5161 acctatcatc gtgtcaatga accagttgct aaggctgaat tgacctttga ccagcaaaat
     5221 tatttgattg gtgctgcagt gattagcgag caagcagacg aattgatcga tgatttgaca
     5281 ctcgtcatca atcaaaaatt gactaaaaaa gagttggata gctatatcat ggggtatccg
     5341 acattggcaa gtgatctctc ctatttattg aaataatcag catgataact aaagcgcttg
     5401 ttgctttttc cttcttgtgc tacactttaa aaaatgaata cctgtttctg gggatgtcgt
     5461 agacgacatg agagtaagtt gtgaacttag acccattgaa cctgtcagtt aatactggtg
     5521 aagggagaaa catagcagac gcttgaagcg ggttactatg tcgtgcccat ttcaagcgtt
     5581 ttttgttatt catattagaa gaaaggaggt aacaaaatga agaaaaatct ttatccagca
     5641 gtcagtatgg cattcatttt aattatttgg cagattgttg tcatagtttg gaaaatacct
     5701 gcctttattt tacccggacc tgctgctgta ttttctgcat tagcggcaga tgcacaagta
     5761 ttactttccc attctgtcac gacattaaat gaagcagtgc ttggtttggt aatagctagc
     5821 gttctttctt ttattactgc cttggcaatg gattattgga agtttttaga aatgtctttt
     5881 tatccgttac tagtgatttc acaaatgttg ccaatcatgg tgttagggcc attacttacc
     5941 ctatggtttg gtttcggtat gcttccaaaa gtgatccttg ttgtcttgat aagctatttt
     6001 ccgatcgtag tttctttttc ggatacattg aggaaagtat caaaagagca aatcatcttt
     6061 ttgaaaacga tgggggcaga tacagggaag atttatcgaa tatataaaat accagcgggt
     6121 ttatccggct tcttttccgg tttaaaagtg gctgctactt attgtgtcag cggagcagtc
     6181 gttggagaat ggctaagcgc tcaagcaggc ttagggtatt acatgatacg cgtaaaaaac
     6241 agctaccaga tcgataaagt ttttgctgcg atcatttgtg tcattctatt gagtttattg
     6301 ctgaatggcg cttgttcatt attgaaaaaa gggtattatc gtatgcttta tattttttaa
     6361 gagaagggga tcaaatgaaa aaattatggc ttttattacc gctactgttg cttttatccg
     6421 cttgtggcac tgcaaaagaa acaagtagca aacaagaaat aaaggatttg aaaaaagtga
     6481 cacttgtttt ggattatgta ccgaatacta atcatactgg catctaccta gcaaaagaaa
     6541 aaggctatta taaagaggct ggtttaaatg tccaaatcat cgaaccagga gataattcga
     6601 caagtattgg tctagtaggt gcggacaaag cacaatttgg tgtaagttat caagaagatg
     6661 tcacttatgc tcacgcagat ggacagaata taccagtcaa agcaattgct acagtgatca
     6721 aacataatac ttctggattc gccaccctat cagacagtaa catccattca ccaaaagatt
     6781 ttgaaggaaa aacatatgca ggttggcaaa gtccaagtga agaagctgtc ttaaaagctg
     6841 tgatggaaaa agacggcggg gacttttcaa aactgacaat ggtaggaagc aatggagaag
     6901 ggccagaaag cttaggaaaa tcctcagata tccaatggta ctttgaaggt tgggatatga
     6961 tcaaagcaaa agaagcgggt attgaactaa actatatccc attgaaagaa ttagatgaac
     7021 ggttagatta ctatactccg gtaattatta cgaatgatca actgatcaaa agtgatccag
     7081 aattggtaca gtcttttatg gatgccacga aaaaaggcta tcaggaagct attaaggatc
     7141 caaatgacag tgctaaactg ttgcaaaaat atgcaaaaga aaatgatcgg acgtttttag
     7201 aagaatcaca agcattcttg tcaaaaaatt atacggatga tcctgagaat tggggattga
     7261 tggaagaaaa agtttggcgc aattatacag cttttatgca ggaaaacgga ttgatcaagc
     7321 aagatgtacc ttcgcaagaa ctattcacta atcagtttat aaaataggag tgatagatat
     7381 gtcaatccaa atcagccact tgaatgtagt cattgaaaac ctgaaaatat tagaagacat
     7441 ccatttcact atccctaaag atacctttac atcgattgtc gcgccaagcg gagccggtaa
     7501 gtcaacattg ctgaaaactt taacaggagt ccagcctatg acttctggaa cgatcaaagt
     7561 agacgatcaa aaagtaactg gtctccatac agcatttagt tatatgccgc aagaagatat
     7621 gttacttcct tggctgagcg tctatcaaaa cgttacgtta tatcaaaaaa tcaatcatct
     7681 aacaatcaat gaagaacaag taagggatta tctggatatc tttgggttaa acggctatga
     7741 acattttttg ccggaacaat tatctggcgg gatgaagcag cgaacggcct tgttacgaac
     7801 aatcatgaat ccttcttctt acttgcttct tgatgaacca tttggagcgt tagatgctat
     7861 gaccagaggg cagatgcaag actggatgtt gaagctgcct aaaaaagcaa agcggactac
     7921 tttacttgtg acgcatgata tcgaagaagc aatttattta tcagacagga ttcttgtgct
     7981 ttccgcccgt ccggcacatg tgattgctga gattcaagtg cctgaaaaac gacggtcacg
     8041 tgaatggcta ctgcagcaat cagagttgaa gcaaacgatt tatcagttat tggcaggtga
     8101 attagatgtt aagtgatgct cattgtcatt tagatgggag tcagagttta gcactccttc
     8161 agcaggaaca cacgattttg acgatcatca attgtgattc gccagaagaa tggaaggaaa
     8221 acagacagct tgcagcaagt aagactcaag ccttaagtta cggtatccat ccttggaaag
     8281 cagatagcta tacgtttgaa caagttgagc cttttttgaa aaaagcaagg atcatcggtg
     8341 aaatagggtt agataatatt tggacgaatg taccgatgac tacacaaaaa aaagtatttg
     8401 agcgacagct tgcttttgca gccataaatg agaaacctgt tgtactgcac acaaaaggat
     8461 gcgaaaaaga gatcttaaat tatatccaag cttaccctaa tcactatttg atccattggt
     8521 attcttcttt ggactatcaa caagactata ttgatttagg ctgttatttc agcattggtc
     8581 ttgatttgaa aaagaatcca gcagtctggc aactagctaa agcagtccca atcgaccact
     8641 tacttattga aacggatgga ctacatgcag cagagtgggc tttagggggg attttttcag
     8701 aaaaagatta tgtctcactg cttgaagaac agttaaagac aatcgctgag atcaaagagc
     8761 tatcagttga agaagttaaa aaaaatactt atcaaaactt ggaacgtttc attagattag
     8821 gtaaataaaa aagagatgtg acacaactag tgtcgcacct ctttttggct ttctttgtat
     8881 aaaagtaaac tgtttgatta ataaatgaat cttagtacca accgtttgct tcccagaatg
     8941 ctttcgccgc ttcccaagaa ccataacgtg aagtaacata ttgttctgct actctttctt
     9001 ggtttgcagc agagtagtca ccatttaagt aagaagaatc taattggtaa cgtccgatgt
     9061 aacgtccgtt cgttgctgtg taagaaccgc ttgattcttt ttgtgcgatc cattcttttg
     9121 cagagcttgt gcttgctgga gtagcagcag tttctgttac agcaggagca ggttgtgttt
     9181 cagc
//
LOCUS       DACEXQ010000100         9104 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.100, whole genome shotgun sequence.
ACCESSION   DACEXQ010000100 DACEXQ010000000
VERSION     DACEXQ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.100"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..1686
                     /locus_tag="IXH04_001638"
     CDS             <1..1686
                     /locus_tag="IXH04_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1852242.1"
                     /translation="RTFIVAILFAFALSVINVLLPRIIQTFMDDHLAKQSATTQVILF
                     FAGVYLFGVIVKSIIWFFQWYLYSMASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGST
                     VSRVTNDTETLFEFWYVFLMVLTGIFAVVSSFVAMFQINGEIALWTLVFLPILGIVIW
                     YYQKFSSRIYRNMREKLSQLNTKLNESISGMRIIQQFRQEARLAKEFEQVNNEYLDTR
                     YAMIKTNSILLSPIINFLYALAIALALTLFGIDALHSPVEVGLIYAFTTYVQEFFNPM
                     SQMMDFLSTFTDGMVAGSRILKIMDTEEYAPQQNPGADQAITSGKIEFRNVSFSYDGE
                     HNVLNNISFVANPGETVALVGHTGSGKSSIINVLMRFYEFYEGQILIDDHDIREYPME
                     ELRKKMGLVLQDAFLFYGDIAGNIRMMNPAITDEQIRHAAEFVQANKFIDELPKGYHS
                     KVIEHGASYSSGQRQLITFARTIVTDPKILVLDEATANIDTETEGLIQEGLAKMRQGR
                     TTIAIAHRLSTIRDAELILVLDKGHIVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            2021..2873
                     /locus_tag="IXH04_001639"
                     /pseudo
     CDS             2021..2873
                     /locus_tag="IXH04_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            2970..4856
                     /locus_tag="IXH04_001640"
     CDS             2970..4856
                     /locus_tag="IXH04_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1852243.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAADGMIRGLTAFLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEADETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMSLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVINYMDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(4897..5799)
                     /locus_tag="IXH04_001641"
     CDS             complement(4897..5799)
                     /locus_tag="IXH04_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1852244.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            6029..7432
                     /locus_tag="IXH04_001642"
     CDS             6029..7432
                     /locus_tag="IXH04_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ1852245.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            7432..8865
                     /gene="ascB"
                     /locus_tag="IXH04_001643"
     CDS             7432..8865
                     /gene="ascB"
                     /locus_tag="IXH04_001643"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ1852246.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            9010..>9104
                     /locus_tag="IXH04_001644"
     CDS             9010..>9104
                     /locus_tag="IXH04_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ1852247.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIE"
BASE COUNT         2862 a         1550 c         2050 g         2642 t
ORIGIN      
        1 agaacgttca tcgttgctat cttatttgcg tttgccctgt ctgtgatcaa tgtcctattg
       61 ccaaggatca tccaaacttt tatggatgat catctggcta aacagtcagc tacgacacaa
      121 gttattttgt tttttgctgg tgtgtacttg tttggagtca tagtcaaaag tatcatttgg
      181 tttttccaat ggtatttgta ttccatggct tcattgaaga cctatcagta tgttcgggta
      241 agattatttg aaaagctgca tacactaggt atgcgttatt ttgaccagac accggcagga
      301 tcgaccgttt ctcgagtgac gaatgacaca gaaacgctat ttgaattttg gtatgtcttt
      361 ttgatggtat tgactgggat ctttgcagtc gtttcttctt tcgttgccat gtttcaaatc
      421 aatggagaaa ttgccttgtg gactttggtg ttcttgccga ttttaggaat cgtcatctgg
      481 tattaccaga aattcagttc tcgtatttac cgaaatatgc gggaaaaatt aagtcagctg
      541 aacacgaaat tgaatgaatc gatttccggc atgcgtatta tccagcagtt tcgccaagaa
      601 gctcgtttag ctaaagaatt tgaacaagtg aacaacgagt atttagacac gcgctatgcg
      661 atgatcaaaa cgaattcgat tctgttaagt ccaatcatca attttctcta tgcattggcg
      721 attgcgttgg cgttgacttt gtttgggatc gatgcgttgc attctcccgt agaagttgga
      781 ctgatctatg catttacgac ctatgtccag gaatttttta atccaatgtc tcaaatgatg
      841 gactttttga gcacctttac agatgggatg gttgccggta gtcgtatttt gaaaatcatg
      901 gatacagaag aatatgcacc gcaacaaaat cctggagcag atcaagcgat tacttcaggt
      961 aagatcgagt ttcgcaatgt aagcttttct tatgatggag agcacaatgt attgaacaat
     1021 atctcttttg tcgctaatcc gggtgagacc gttgcattag tggggcacac cggaagcgga
     1081 aaaagctcga tcatcaacgt attgatgcgg ttttacgaat tttatgaagg ccaaatcttg
     1141 attgatgatc atgacattcg agaatatcca atggaagagt tacgtaagaa aatgggattg
     1201 gtcttgcaag atgccttctt gttttatgga gatattgcag gaaatatccg gatgatgaat
     1261 ccagctatca cagacgaaca gattcgccat gcagctgagt ttgttcaagc aaacaagttc
     1321 atcgatgaat taccaaaagg atatcactca aaagtgatcg aacacggtgc cagctattct
     1381 agtggacaac gacagctgat cacttttgcg cgaacaatcg tgacagatcc taaaatacta
     1441 gtattggatg aagcaacagc aaatatcgat actgaaaccg aaggattgat ccaagaagga
     1501 ttggcaaaaa tgcgacaagg acggacaacg atagcgattg cccatcggct gtccacgatt
     1561 cgggatgcag aattgatttt agttttagac aaaggtcata tagtagagcg tgggaatcat
     1621 gaaacacttc tagcgcaaaa tggtttgtat gcagatatgt acaaactgca aagcagtggt
     1681 gtgtaaaaag aggttgtgac agtagcatag tagcattttg ccctaagcgc tagagtgaaa
     1741 ataaaacaga gcttcgcaga ttcttcatct gtgagcgtta gtggcgtagt cacccgattt
     1801 aaaaaagacg acgacagcta gtcaaaagcg gctaataacc actaagaaag cgttgacaaa
     1861 agcgtttgct ttcgataata taaagaaaac gatacaactt ggatagtgat tgcctgatgc
     1921 aagctaaacc tgatgagtct cccatggttc catggacggc tttttgggtt tttctttttg
     1981 ttttggatac agccctatat atcatggaaa ggagcaaaac atgctgattg agaaagtact
     2041 gaataacaac gtcgtaataa ccagggatga aagaaatcag gaaatgatcg tgatgggaaa
     2101 aggtttggca ttcaaaaaga aagtgggtga cccgattttt gactcatgca tcgacaaaaa
     2161 atttcagttg acaaatgacg ggctttcaca acagttccaa gaattggcac aagcgattcc
     2221 tcttgagtac ttagaaattt cttgtgaaat cattgaatat acaaaagagg ctctacaagt
     2281 ccagttgaat gattctgtgt atattgcatt gacggatcat ttgtatacgg ctatcgaacg
     2341 tcacaaaatg ggaatatttg taccaaatgt attgctgtat gatatcaaac aactatttcc
     2401 taaagagtat gccatcggga aaaaaaacag tgaagaaaat ctcggataaa taccacatca
     2461 agttatcaga agatgaagcc ggatttatcg cattgcattt agtcaatgca caaggcattc
     2521 aaagccaaca agacaatacc tacctgttca ctataacaat acaggatatt ctaaatatcg
     2581 tccgctatta ctataagatt acatttgatg aagattcagt ccattttttt cgattcgtca
     2641 cacacttacg tttttttctg acacgcgtcc tagatggaga aaaatcagaa gtgggagaag
     2701 tttctgagga attgtttcag gttattcaaa aaaaatataa ggatacgttt ggttgcgttg
     2761 agaaaatagc agacttttta gccgagacgt tcaactacca gatgtccacg gacgaaaaaa
     2821 tgtatttaac gatccatact gcgcgtttag tgcatgagaa tcgttgtaaa taaataaaaa
     2881 aaggaggata aattaacaag aacgtatgaa ataagggtag tgacattcag ttggctaaac
     2941 cttccactaa taaattggag ggaatagaaa tgggaaaata ccaagaatta gcgagaaaaa
     3001 tcgtagaaaa tgtcggcggg aaggaaaata tcaatggttt gacacattgt atcacaaggc
     3061 ttcgtttcaa attaaaaaac gaagaaaaag caaatgatga gatcttgaaa aatatggacg
     3121 gtattgtaac ggtaatgcga gcgggagggc agtatcaagt tgtcatcggg aatcatgtgc
     3181 cagtcgtgtt tgaagaggtt ataaaagcag gaaaccttac ttttgatgag gctgtttcaa
     3241 cagaaaaaat gagaccgttt gatatgctta ttgatggaat atcgggctgt ttccaaccat
     3301 tccttgctat cctagctgct gacgggatga ttcgtggact gacagcattc cttgtcttct
     3361 taggcgcgtt tgaccgcggt tctggcacat ttgttatgtt tgataatatc ggtgattctg
     3421 tattccaatt tatgcctgtt attatcggtt taacagctgc ccgtaaattt aaagtaaatg
     3481 aattcgtagg gatgttgctt ggcgcagcat taatgaatcc tagcttgtcg ctggaagctt
     3541 tgagtggtgc agcagaagca ccactaacga ccatattttc tggcactatt tttgaagcac
     3601 ctatttatca aacagtattc ggcattccct ggattgcgcg taactacgct tcttctgtca
     3661 ttcccattat ctttattgtg ttgttagcta gccaaattca gaaaccaatt aaaaagttag
     3721 tacctgagat gatcgcgaat ttcttcgttc cattcttcac cgttcttatt acaatgcctt
     3781 taggtttttt actcgttgga ccagtattca cattcgcaac tgatatttta atggctggat
     3841 ttgaaacact tttagcactt tcgccagtta tttatggagc aatcgtaggc ttcttctggc
     3901 aaattctggt gatgtttggt ctccattggg caatcgtacc aatgggattg atgcaatttt
     3961 ctgtgaatgg ttggcaaaat attatgacac ctgttgcagt agtgtctttt ggacaaactg
     4021 cagcacttac cgcactctac tttaagctta gaaatccaaa agacaaagca attgcaatac
     4081 cagccattgt ttctggtatt gtggggatta cagagccagc tatttatggg tttacattgc
     4141 cacgtaaaaa aatatttatt tttacctgtg tcggtggtgc gttagggggc gcgtatagtg
     4201 ggttaatgaa tttaacttct tggaatcaag gtggactagg catctttaca attcccaact
     4261 atattagacc agatggtgat ttaacagatg tgattaatgt attgattggg atcgccattg
     4321 cgatggtggt atcctttgcg ctgacttttt tcttttggaa ggaggaagca gatgaaacag
     4381 atgatagaca gaaaagatca ggtaaagaaa ttgtaaaaac accgatacaa ggacaaatcg
     4441 ctccactgaa tgcagcaaag gatgcagctt ttgcacaagg aacactaggt cgaggaatct
     4501 tgatctaccc tgaaaaagga gaagtacgtg ccccttttga cggaacagtc atgtccctgt
     4561 ttccgacgaa acatgcaatc gggatggttt ctgaagcagg tctggaattg ctgattcatg
     4621 tcggattgga tactgttcag ttggaaggga aatattttga aagcttggtg cagcaaggag
     4681 aaagagtgaa acaaggcgat ctgttgcttc gcttcgacaa aaagaaaatc gaagaggaag
     4741 gatacagttt agaaacacct gtgattgtca tcaattacat ggattatttg gatattttgg
     4801 aaaatcaagg ggaaacggtc gggccagacg atgcacttat caccgcactg acctgaacgg
     4861 ttaggtgaaa gaatggacta ggcttaacta ttctttttag tccacttctt tattttcaaa
     4921 attgaaaggc tctttcatga tggctgagct gatagggcgt ttatcaaatt tttgtcctac
     4981 agaaattaga tggactaaat tctgttgata attttctgca aaaacacaac tatataacac
     5041 gtctagaaga taacaaatcg atgtattgat cgtaaaatta ccaattttcg aatacagtcg
     5101 ttcgcgtgtc gtaagtcgca aagcggctgt actcattgaa gatagcgttg aatcaccgat
     5161 actggtcaaa gctaaaattg gcacatgatt ttgtttcaat atttctgcgg tacgaaggat
     5221 cacattgttt tcacccgtat aagaaatcag aatcccacac atatcacttg tgcaattgta
     5281 cgcttcatag aacgattcac caaaagtggt gacaatcgtg acatccattt tgatgcgctt
     5341 cattttcagc atgaaatctt gagaaatcaa agtattcgag ttactggcga aaatcacgat
     5401 tttttttgct tgaagcatta gttgtttggc tttttgcagt tggtcatgat gaatgagaga
     5461 aagtgtatct tgaatcgtca tctgttctaa agaagccatt ttatttgcga tcgtcataat
     5521 accgtctgtg gtggaaaaag gaagattcgc atctacgtct tgaaaattcc cttcaagata
     5581 ggctgtttca gctaaaaaag cattacgaaa actgacccag ccatcaaaac ccattttttt
     5641 tgctacacgg atgagtgtgg acgggtggac ataacatcgc tcagcaatct cttggatggt
     5701 catttcgcca agtgctggag aatcgcttaa gagaaactga atcacttcat tttctgaagg
     5761 agaaaaggag atttctttga ttttttcacg taataacatg tagatacctt cttcttgttt
     5821 ttttacgtaa attataactt ttttgaaaat gatttgtaaa cggatttatg ttatatagct
     5881 taataaagac attttttgac aagatattta caaaaatcca gaagaaaaca ctaacttttg
     5941 taaatgttac tggaaagaaa cgaatttcta ttgtttgttt tgtttacaaa aaagataata
     6001 aaagtgtaaa aagtaaagga gtgagaacat ggcaaaagga atcaaaatcg ctacgattgg
     6061 tggaggcagc agttacaccc ccgagttgat ggaaggattt atcaaaagat atgaggaatt
     6121 accgatacga gaaatctggc tcgtagatgt agaagaagga aaagaaaagc ttgagatcgt
     6181 cagtcagatg tctcagcgta tgtgggatgc ttctccgtat gaagtcaaaa tctatgcaac
     6241 tctggatcgg gaagaagcat tgaaagatgc ggattttgtt acgactcagt ttcgtgtcgg
     6301 tcaattagat gcacgaatca aagacgagcg gatccctagt tattatggga tgattgggca
     6361 agaaacaaat ggtgctggcg ggatattcaa agcttttcgt accgttccag tggttttggc
     6421 aatcgttgaa gacatgaagc gtttatgtcc aaatgcttgg ctgattaatt tcgcgaaccc
     6481 aagcgggatg gtgacagaag cagttatcag atatggcaaa tgggataaag tgatcggatt
     6541 atgcaatgtg ccagtaatgg cgatgatgac agaaccaaca atgcttggca agaaaaaaga
     6601 agagctgatc tacaagtttg ccggtctaaa tcacttccat tggcacaaag tagcagataa
     6661 taaaggaaat gaccgcacgg cagaattgat tgatcgttta tacgatgaag acaatggatt
     6721 gccgaaaaat atctttgaca tcccgttttt caaagaacaa ttaaaacaaa tgaacatgat
     6781 cccttgtggc taccaccgct attactatcg tgaagaagaa atgctggaac atgctttaga
     6841 agaatatcaa acgattggta caagagcgca gcaagtgaaa gaaacagaag cagagctgtt
     6901 tgaattatac aaagatccag aattggatca taaacctgaa caattgcaaa aacgaggtgg
     6961 tgcttattat tcagatgctg cctgtgaaac gattgcttcg atctatggaa ataaagaaac
     7021 acagatcgtg gtatctacaa agaatgatgg agctgttcct gatctgccgg cagattgtgt
     7081 agtagaagtc actgcctatg ttggcgcaca aggcgcacga aatgtagcgt ttggttccct
     7141 tcctgctgct gaaagaggct ggttgcaagt aatgaagaat atggaattgc tgacgatcga
     7201 agcagccgtg actggtgatt atggaacagc tttgcaagcg tttacgatca atccgcttat
     7261 acgatcagga aagacagcaa aacggatcat ggatgagttg tttattgcac atcagcagta
     7321 tttaccgcaa ttcaaggaaa caatcagccg gttaaaagca gaaggaatcg aagtccaaga
     7381 tgaactggca agagaattat caagcaatac acatgtaatg gaggaagtat gatgggattc
     7441 agaaaagatt ttttatgggg cggagctaca gcagccaatc aatgcgaggg cggttatgat
     7501 gaaggcggac gtgggttagc gaacgtagat gttgtgccaa tcggagaaga ccgttttcct
     7561 gtcattactg gaaaaaagaa aatgtttgaa tttgatgaaa atcattttta tccagcaaaa
     7621 attgcgatcg atatgtatca tcattataag gaagatattg ctttatttgc agaaatgggg
     7681 ttcaagactt atcggatgtc cattgcttgg agtcgtattt ttccaatggg cgatgaaaaa
     7741 gagccaaatg aatctgggtt gaaattttat gaggatgtct ttaaagaatg tcaaaagtac
     7801 gggatcgagc cgcttgtgac gattacacat tttgattgtc cgatgcattt gatcaaagaa
     7861 tatggtgcgt ggcgttccag agaactcgtt ggtttttatg aaaatctgtg ccgagtcatc
     7921 ttcaatcgct ataaaggact tgtcaaatat tggttgacat tcaatgagat caatatgatc
     7981 cttcatgcac cattcatggg cgcagggctg tattttgaag aaggagaaaa tgaagaacaa
     8041 atcaaatatc aagcggcaca tcatgagttg gttgcaagtg ccatcgcaac gaagatcgct
     8101 catgaagtag atccggagaa caaagtaggc tgtatgctgg cagcaggagc atattatcca
     8161 tacacttgca atcctgaaga tgtttgggct tctagaaagt cagatcgaga aaattatttc
     8221 ttcattgatg tccagtcacg aggagaatat cccgcttatg cattgaaaga aatggaacga
     8281 aatggcattc agatcaaaat ggaacctggg gatgccgcat tgctgaaaga acatactgtt
     8341 gattttatct ccttttctta ttattcttct cgagtttcga ctactgacaa cgaactcttg
     8401 gaacagactg caggaaatat ctttgcttct gtcaaaaatc catatctgga agcaagtgaa
     8461 tggggttggc aaattgatcc attaggcttg agaataacga tgaatgatat ttatgatcgt
     8521 tatcaaaaac cactatttat tgtagaaaac ggcttaggtg cagtcgatac gcccgatgag
     8581 aatggttatg tggaagatga ttatcgaatc gactatctgg cagcacatat ccaggcaatg
     8641 aaagatgcag tagaattaga tggagtcgat ctattaggct atacgacttg gggatgtatt
     8701 gatttagtat cagctggtac aggtgagatg aagaaaaggt acggattcat ttatgtcgat
     8761 cgtgacaacg aaggaaatgg cacattagcc cgttcgaaga agaaatcatt caactggtat
     8821 aaaaaagtaa ttgattcaaa tggagaaaat ctttcgaacg attgatcaat gatttataaa
     8881 agtctattgt ttttcagcgg atttttcgag tataataaag ctattcgaga aacgcaagca
     8941 gaacaataga ctttttttcg cattggctgt aaagtatgct tacttgatta agtaaaggag
     9001 aatctacata tgtatgatgt aatcgttatt ggcgcagggc ctgctgggat gactgctgct
     9061 ttatatgctt cacgttcaaa cttatccgta ttgatgatcg aaca
//
LOCUS       DACEXQ010000101         9101 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.101, whole genome shotgun sequence.
ACCESSION   DACEXQ010000101 DACEXQ010000000
VERSION     DACEXQ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9101
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.101"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..1250
                     /locus_tag="IXH04_001645"
     CDS             <1..1250
                     /locus_tag="IXH04_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAQ1852248.1"
                     /translation="IKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENL
                     IPSAADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKN
                     TFVDRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEF
                     PIVDNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPV
                     QRFPQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLV
                     PFVLDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMI
                     DMLLTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHG
                     RPVLIHFTNSDMERMFKRIQDPH"
     gene            1272..1832
                     /locus_tag="IXH04_001646"
     CDS             1272..1832
                     /locus_tag="IXH04_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAQ1852249.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(1917..3905)
                     /gene="treP"
                     /locus_tag="IXH04_001647"
     CDS             complement(1917..3905)
                     /gene="treP"
                     /locus_tag="IXH04_001647"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAQ1852250.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            4128..4841
                     /gene="treR"
                     /locus_tag="IXH04_001648"
     CDS             4128..4841
                     /gene="treR"
                     /locus_tag="IXH04_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAQ1852251.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            4918..5364
                     /gene="msrB"
                     /locus_tag="IXH04_001649"
     CDS             4918..5364
                     /gene="msrB"
                     /locus_tag="IXH04_001649"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAQ1852252.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            5534..6136
                     /gene="ruvA"
                     /locus_tag="IXH04_001650"
     CDS             5534..6136
                     /gene="ruvA"
                     /locus_tag="IXH04_001650"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAQ1852253.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            6149..7150
                     /gene="ruvB"
                     /locus_tag="IXH04_001651"
     CDS             6149..7150
                     /gene="ruvB"
                     /locus_tag="IXH04_001651"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAQ1852254.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            7179..7757
                     /locus_tag="IXH04_001652"
     CDS             7179..7757
                     /locus_tag="IXH04_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ1852255.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            7809..8291
                     /locus_tag="IXH04_001653"
     CDS             7809..8291
                     /locus_tag="IXH04_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAQ1852256.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            8408..8782
                     /locus_tag="IXH04_001654"
     CDS             8408..8782
                     /locus_tag="IXH04_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852257.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         3060 a         1641 c         1932 g         2468 t
ORIGIN      
        1 aaatcaaaat ggacccgctt ttagtagatg taaatgttca tccgacgaag caggaagtcc
       61 ggctgtcgaa ggaaaaagaa ctaacagagc taattagtaa cgccattcaa aaagcattgt
      121 ctcaagaaaa tctgattcca agtgcagcgg ataatctacg gtttaagaaa aaagtacctg
      181 atcagcctaa aacagaacaa atcgaaatcg atttgtccta cgcttttgaa gaagaaaaac
      241 ctaaaaagcc aagtagtttg aattttgatc gagagacagg tacctttttc gtagaagaac
      301 taaagaacac atttgttgac agaattgttg ataagtctgt ggaaaatgtt gaaaactatt
      361 ctggtttttc tgaagaaaac accacttatc cagaaaaaac agcgaaaacg gtggataagt
      421 ttggtgaaaa tgttgataac tttgtagaaa aaacagtgga aaaatctgaa aagaaagaat
      481 ttcccattgt ggataacatg gagaaaacaa atgaagaaca agcagataaa tccgtagaag
      541 aggatgaaaa gatttacgag gaagaattga cactccatcc tgaatttgat ttcactcaag
      601 gaaacgacaa aacattagaa caagccatca agaaattaga aacggaaaaa ccagtacaac
      661 gttttcctca gttggaatat tttggacaga tgcacggaac gtatcttttt gcccaaagta
      721 gcgatgggtt gtacatcatt gatcagcatg ctgcccaaga gcgcattaag tatgaatatt
      781 tccgggaaaa aattggggaa gtatctgatg atctgcaaga attgcttgtt ccatttgtgt
      841 tagactatcc aaacagtgat gctttgaaat taaaagagca aaaacacttg ctagaagatg
      901 cagggattta tttagaagaa tttggtcaaa acagctttat tgtacgggcc catcctactt
      961 ggtatcccaa tggagaagaa gaatcgatta ttcgggagat gatcgatatg ctgctgacaa
     1021 ctggaagtgt gagtgtaaaa aaattccgtg aagcaacagc aatcatgatg agttgcaaac
     1081 gatcgattaa agccaaccat tatttgaatg aagcacaagc acgtgtgctg ctgaaggatc
     1141 tggcacgatg cgaaaatcct ttcaattgtc cccatggccg tccagtattg attcacttta
     1201 ccaattcgga tatggaaagg atgttcaaac gaatccaaga cccgcattaa gtcaaagaga
     1261 agagggaaaa catgaagatt gtattagcat cccaatcacc aagaagaaaa gagttattgg
     1321 ctcatatcgt ttcagaattc gagattgaac ctgctgatat cgatgaaacg ccatttccag
     1381 acgaacagcc cgtcgattat gttcgtagaa tggctgaaga aaaagcacga actgtctggg
     1441 aacaagatga aaaagaagac catttagtga ttgccagtga cacgactgtt gttctcaatc
     1501 aagagatcat gggtaaacca gaagatctgg ctgaggctga attcatgctg aaaaaactaa
     1561 gcggggaaac acatactgta tatacagctg ttgttttaaa aacaaaggaa cgagaagaac
     1621 ggatactagc agaagcacat gtcacttttt atcctttgac agacgaagag atcaaacgct
     1681 acttggagac gggggattat gcggataagg caggggcata cggcatccaa aatgctgcga
     1741 gtgtattcgt caaagaaatt tctggagatt attacagtat cgtaggattt ccgattggtg
     1801 ctgtcaatca ggcactgaaa acattcattt aaacaaaaaa gcggactatc tgattttcga
     1861 ttgaaaatcc atgatagccc gtttttttta ttgaaaagat tacttatcta ctctgactaa
     1921 gattttactt cagctttacc gactgcttcg cccgggcgaa caagtcctga ttggtcaaga
     1981 gaaagtcctg caactttttc attattggtc aaagcaacaa taatgtcagt tttcttgcca
     2041 gcttgttcta tttctggcaa atccatttca gcaatcaggg tctctggtgt gacaaggtat
     2101 ccttctttca cgaatacatt gaaaggaaca ccctttagtt caacagtatc taatcccatg
     2161 tgaacaagta cttccagccc ctcattagaa agaataccaa tggcatgttt tgtttcaaag
     2221 acactcgtca ctttaccgtt tactggtgca tagactttgc cgtttgacgg ttcgacagca
     2281 taaccgtcac ccatcatttt ttgtgagaaa actgggtcat ttacttcagt tatttccttg
     2341 atggtccctg cagcgactgc gaataatgtt tcttttgttg caactgccgt accagttgct
     2401 gaaacaggtt cgaaagactg tggcgttgct gttgcgccat ttgctgtttg aagttgtaca
     2461 tcagcagcat tttcaggaac agcaggatca atcttattca aaattccctg acgtctaaag
     2521 atgatcgtca agccaaatgg aatgatgaca gcaatgatca ttgcgatgat gaaaggtacc
     2581 catgtttctg cttggatagc tagtattcct ggcaaaccgc ctactccgat cgcgttagct
     2641 cgaacaccca tcaagttagc aaacatgcca gctaatccag agccgaccat tgcagcgacg
     2701 aaaggataga cgtatttcaa attgattccg aacatagcag gttcagttac gcctaagtaa
     2761 caagaaatca ttgctggaat cgagatttgt tcttcttttt tattcccacg atgtaagaag
     2821 atgatggcta agactgcaga gccttgggcg atatttgata aagcaatcat cggccataaa
     2881 ttcgttgatc cgaaatctgc aatcagctgc atatcgatgg cattggtcat atggtggaga
     2941 ccagtaatca ctaaaggagc atacaggaat ccgaatacgg cactaaacaa ccagctgata
     3001 gaagacgtca atccagtatt tacgatattt gagatccagc ttccgactgt ccagccgata
     3061 ggacctaaaa taacgtgtgc agctaaaaca gttggtaaca atgaaaacaa tggaacaaaa
     3121 atcatcgaga tcgactgagg gatatatttg cggaagaaga tttctagata cgctaacata
     3181 aaccctgcca gcatggctgg aataacttgc gcttgataac caatcatctg gacttgagca
     3241 aaaccaaaat cccaaaatgg aatatctgct gcggctgtgg aagctacact ataagcattt
     3301 aataattgcg gagaaactaa cgtaattcct aaaacgattc ctaatatttg tgtagtaccc
     3361 atcttttttg caatactcca agtgatacct actggaagaa aatggaaaat tgcttcaccc
     3421 ggtaaccaca aaaaggcatt gacaccattc caaaacttcg agtgttcaac gattgttcca
     3481 ccaagacttt caaactggat accttcaagt acatttctga atccaaggat caatccaccg
     3541 acaatgattg caggaatcag tggtgtaaag atttctgcta aaacggcaat agcacgttga
     3601 acgggatgaa ggttttgttt tgcagctgct tttccttgtt ctttggaaac cccttcaact
     3661 ccagatactg caacgaagtc gttataaaaa gtagaaacat cgtttcctat gatgacttgg
     3721 aactgtcctg cattcgtgaa catccctttt acgctaggta tatcttcgat cgctttttcg
     3781 tccgcttttc ctggatcatt caggacaaaa cgcatacgag tcgcacaatg actaactgca
     3841 gcaatatttt cttttccgcc gatatcatgt aacagcttct cggcatctgc ttgatatttt
     3901 gccatatcaa taaacccctt tctcttaact tgtctatgca agttgtttct tcatttacta
     3961 tacttttttt cggagagaat tgcaagcgtt ttattaaaaa gtacgagaga aaatgatatc
     4021 gttttaaaaa gcttaaaata agcttttctt aaaaatagga acattgaaat acaaaaagag
     4081 ctgtagtata gtgaagaaat gatggaagaa cgagtgaggg aaaaaacatg aataagttca
     4141 atgaaatttt tttagatttg gaacagaaaa ttttagcgaa agaatatcct ccgcattcgc
     4201 ttttacccag tgagaaccaa ttgatcaaaa tttatggtgt ttcacgagaa acaattagaa
     4261 aagcgctgaa tcttttgact acggaaggtt atattcaaaa aaaacaagga aaaggttcga
     4321 tcgtactgga tcggaatcgc ttcgactttc ctatttctgg attgactagc tataaagagc
     4381 tacaggagac ccaacgtatt ccaagtgaaa cgatcatcca tacgttagaa gaaacagaag
     4441 tgacaaaagc attgaacgag atcactggct gggagatcgg tgcgcctgtt tggcatttga
     4501 tacgcgaaag aaaaatcaat ggcgaagtag ttatattaga caccgattat ctattaaagg
     4561 aaatcgttcc tattttaact ccagcacaag cacaaggatc gatctatgaa tattttgaaa
     4621 atgagctttc tttgacgatt gcttacgcgc aaaaggagat cacagtagaa gaagtaacgg
     4681 aaatcatgaa aacagcgatg gatcttcacg atgacactta tgcagtagta gtcagaagcc
     4741 tcgttcatct ggaagatacc cgctgtttcg agtattctga gtcggttcac cgattagata
     4801 aattccgttt cgttgagttt gcccgacgcc gaaaagtgta aatagttcaa aagaaagcaa
     4861 attgttggca tctgtccact ttggcgctat gataggatca caaagggggc atttttaatg
     4921 gataaaccaa taagaccaga aaaagaagaa ctgaaagaaa agttatcacc catggcttat
     4981 gcagtcacac aagaaaatgc aacagaacga ccatttagcg ggaaatatga tgatttttac
     5041 gaaaagggga tctatgtaga tatcgtcagc ggtgaaccgt tattcagctc tgcagaaaaa
     5101 tatgatgcag gttgtggctg gccatcattc agcaaaccaa taacgaaaag acaagtgcgc
     5161 gaaaaagccg atttttctca tggcatgcat cgaatagaag ttcgaagcaa agaagcagat
     5221 tctcatctcg gacatgtgtt taccgatgga ccagtagatc aaggtggtct acgttactgc
     5281 atcaattctg cctcactgaa attcattcca tatgaaaaga tggatgaatt aggttatggg
     5341 gaattcaaat cacttgtaga ataatggaaa tacacaaaga gcggtacaat acggggattt
     5401 actcgatcgt ccgctctttt tttattcaag cgattgctat ttgaaagaaa cgctccctag
     5461 actccttttt tcacaaggtg aaatgtgcta caataaaggg aacgtatgtg tgcaaaagaa
     5521 agaggactag aatatgtatg aatatctgac aggtgtggtt acctttatca acccctatta
     5581 catagtagta gaaaccaatg gtatcggcta ccagatcgca ctaggaaatc cttaccgcta
     5641 cagtagcaaa ctaaatcaaa aaataaaaat ttatgtccat caggtaatac gtgaagatgc
     5701 gcatcttctg tatggatttg attcgctaga agaaaagcaa ctgtttttgc gattagtaag
     5761 tgtttcagga atcggtccga aaagtgccct tgcaatcatg gcaagcgaag accactccgg
     5821 tttgattcaa gcagtagaat ccagtgatgt cacttatttg acgaaatttc caggagtagg
     5881 gaaaaagaca gcacaacaaa tgatcttgga tctaaaagga aaattcggtg agctgagtat
     5941 cgatacacct tttagtctat ttgatgaagc aaacacaaaa gacgctacag ctctatctga
     6001 agcaatggaa gctctctcgg ctcttggtta cagtgatcga gaaatcaaac gtgtagaaaa
     6061 acaattaaaa gaagtagata atcagacaac ggatgagtat ctacgccaag cactcaagct
     6121 aatgatgaaa aaataaggag gagaccaaat gacagatgaa tacttgttat caccagaatc
     6181 tggcgaaaat gaactgagtt tggaaaaatc tcttcgacca cagctgcttt ctcaatatat
     6241 cggacagaga aaagtcaaac aagaacttca gatttacatc gaagctgcca gaaatcggga
     6301 agaaccttta gatcatgttc tgttgtacgg gcctcctgga ttaggaaaaa cgacgatggc
     6361 catggtcatt gctaatgaga tgaccgtaaa tattcgtacg acgagtggcc ctgctatcga
     6421 acgtccgggt gacttagtag caatcttgaa tgaactggaa gcaggggatg tcctgtttat
     6481 cgatgagatc catcgattac ctagagtggt agaagagatg ctttattcag ctatggaaga
     6541 tttctatgta gatatcatgg taggacaagg gccaaccgca catccggttc atttccctct
     6601 tcctcccttt actttgattg gcgcgacgac acgggcaggt atgctttctg cacctttacg
     6661 agatcgattt ggcatcattt ctcatatgga atattatgac gaacaggatc tgcaggagat
     6721 cgttttacgt tcttcggata tttttcaaac agagatcatt gaagaagggg cattggaaat
     6781 tgctcgccga tcacgaggaa cgcctagaat cgctaatcgc ttgctcaaac gggttcgtga
     6841 ttttgcgcaa gtccaaggag acgggaaaat cgatcgtcct atagcggatc aggcacttac
     6901 tttgctacag gtcgatcagg ctggattgga ttatgttgac cagaaattgt tgcgaacaat
     6961 gatcgagtta tatggcggcg gaccagtagg gttaagtacg ctttcagtaa acataggaga
     7021 agagcgtgag acggtagaag atatgtatga accgtatctg atccaaaaag gatttttaaa
     7081 gcgtacacct agagggcgaa ttgctactgc ttatgcctat gaacattttg gttacgacta
     7141 tcatatatga tttttgcttt tagattggag gaaaatgaat gagtgataca acacgtacga
     7201 aacaattgat caaaaacgta ttgattgatt tgtgccaaac gcaatctttt cgaaaaatca
     7261 gcgtccaaaa aatcgcaaac gaagcaaaaa tcaatcgaca aacattttat tatcatttta
     7321 cagataaata cgatttgctt cgttgggtct attgtgagga tgcattgcat tatttagatc
     7381 cacaatgtct ttcacttgat aattgggaag agcaagcatt gaaaatgctg aaagcaattg
     7441 ctgagaagaa agaattttat gcaaatactg tgctttctga cccagaaatt ttacaaaaag
     7501 agttttctca actgatccaa aaatcattta tcaagatttt tgaacaaatg gatgaagaaa
     7561 gacaactttc tgcaagagac aaactatttt atgctcgttt tttttcttac ggttgcagtg
     7621 gtgtattagt caattggatc acgaatgaat atgaagaaac accgatggaa atcgctatgc
     7681 agcttttccg tttagccaag gatacggaat tatattctta tcgtctttat gcaaaagaag
     7741 agttggaaga acagtaatag aaaaattttt tgttggaaaa gcaataatcg ctaaaaggag
     7801 atggggaaat gacaaaaatt ttatttgttt gtttgggaaa tatttgccgt tcaccgatgg
     7861 cagaaggctt gctgagaaaa cgaatagcat tagaaggaag ggaagcagaa ttttttgtgg
     7921 attctgctgc tacaagcacg tatgaagttg gaaagacacc gcaccctgga acgaaaaaaa
     7981 tattaaacca agaaaaagta gatatgacga atatggttgc tcggcaaatc acaccccacg
     8041 attttgaaac ttttgattgg atcattggca tggatcaaga aaatgtagaa gaactgaaac
     8101 gaagagcacc acgctcagcg caggggaaaa tccatttgtt tttaagtagc attcctggaa
     8161 aagaaaaaga aaatgttccc gacccatact ataccaataa ctttgatgag acatatcact
     8221 tgatcacaga aggcctgaat cattggtgga atatttggat tgatgaaaat aaaataacaa
     8281 ataatttgta aagttaacgc tttcttttta aggcgatgtt agagcatttc cttgtttttt
     8341 tgtaaaatgt taggtaaaat aaaattaatt aagtaacact ttcttagcta aaggaggagt
     8401 gcattcgatg aacgaagaga cagtatcgcc aatgaagaag cttacgaagt tagttactca
     8461 tacttcgtat caactttttg attcttttgt aaatgacact gaagaacatg ctaagaatgt
     8521 acgattgcaa ggaaagttta ttcttgagct gcaaaaagct gctactgaac atagtctagt
     8581 tatcttacaa gtaagagcaa atcgtttgag tagcaagtat gaaacgatct caggctgggt
     8641 ggcgacaaaa acggtcgaaa aagaccgagt gatgatccgc ctacaaaacg aagagcagca
     8701 gatccggatg gttcctgtag aacaaattga aaaaataaca atcctttcac cagcagggga
     8761 acaggaacgg ttatcaaaat aatttatacc agatagaaaa gcacgtatga gcttgtacca
     8821 tcaatcatta ctaatcaaaa ataaccgaat gctttaagct gatatcagta aaacggaacg
     8881 ttttaggatg agtatcatag catgacagca ttcggttgat tagttgaatc gattggatgg
     8941 tacaagtttt tttgataaaa aaaggtcctg ctagccgggg agctagcagg ataaggagtt
     9001 aaaaatgaaa aagtgttgtt agggttgttt gttggtatgc ttatataata gtcattagtt
     9061 gtgaatattt tgtgacgaaa gttttcgaaa gttgttaagt t
//
LOCUS       DACEXQ010000102         9070 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.102, whole genome shotgun sequence.
ACCESSION   DACEXQ010000102 DACEXQ010000000
VERSION     DACEXQ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.102"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            303..1151
                     /locus_tag="IXH04_001655"
     CDS             303..1151
                     /locus_tag="IXH04_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1852258.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            1382..1861
                     /locus_tag="IXH04_001656"
     CDS             1382..1861
                     /locus_tag="IXH04_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852259.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            1894..4122
                     /locus_tag="IXH04_001657"
     CDS             1894..4122
                     /locus_tag="IXH04_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ1852260.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            4251..5894
                     /locus_tag="IXH04_001658"
     CDS             4251..5894
                     /locus_tag="IXH04_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ1852261.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(5967..6512)
                     /locus_tag="IXH04_001659"
     CDS             complement(5967..6512)
                     /locus_tag="IXH04_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAQ1852262.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(6535..7020)
                     /locus_tag="IXH04_001660"
     CDS             complement(6535..7020)
                     /locus_tag="IXH04_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1852263.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(7010..7822)
                     /locus_tag="IXH04_001661"
     CDS             complement(7010..7822)
                     /locus_tag="IXH04_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAQ1852264.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(7961..>9070)
                     /locus_tag="IXH04_001662"
     CDS             complement(7961..>9070)
                     /locus_tag="IXH04_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAQ1852265.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT         3071 a         1693 c         1795 g         2511 t
ORIGIN      
        1 tgtcaatgga atcaaaaagc tatgcaatga tttattgtgt ggctgtagga agtattggcg
       61 gcttagtatt tttattttct caattcggaa aaaatgggtt tcgaatgttg aaaaatgtta
      121 aacatcatag cagaagcaaa gaataggtta actaatcgtt ttttgctgtc acttgaaaaa
      181 cggcaactcg tatgattacg cttgtcgttt ttctattttc caattttagt ttcatatcta
      241 atcttactta tgatatagtt ataaggtctg gaaaaattct ttgaaacgag gaaattaaac
      301 acatgaaact tgcagtagtt acagatagtt cggcatatct gccggaacgt atcaagaacc
      361 atcccgactt attcgttatt ccaatccctt tgattatgga tgggaaaatt tataacgaag
      421 gaatcgatat cgaagcggat gaatattatg gacttttaaa aaacagtaaa gattttccta
      481 ctacttcaca acctgtcgta ggggaagttt tgggtttata tgaggaattg aaagagaaag
      541 ggtatgacac gattttaagt atccatcttt cttcagggat ttctggcttt gtcaacacat
      601 tatttgcgat gaaagatgac atcagcggag taacggtcat tccttatgac tctaagatca
      661 ctagtatgcc gatgggacac atggtagaag ctgccttaga cttaaatgat gaaggtcagt
      721 cccttgatga aatacttgca catatcgatc gaatccgaga taatacctac gcttatctga
      781 ttgtagatga tttgaacaat ttagtacgag gaggacgttt gacaaatggt gcagcgctga
      841 ttggcagctt attaaaaatc aaaccgatcc tgacattttc tgaagggaaa attgttttat
      901 tcgaaaaaat ccgttcaagt aaaaaagcat ttgctcgagc tgaagaaatt attggtagaa
      961 gaaataacga aatacaaccg ccagtcaaac tttacgtgat acatgcaaat aatttagaag
     1021 tagcgattgc ggagaaagag aaattgcaga aaaaatatcc aaatgcagaa atcgaaattg
     1081 gtcatttcgg accagtcatt ggtacacatt taggtgaaaa agccatcggc attgccattt
     1141 cagcgcaata attcctttta ttcaaaaaac ataccaaaaa tattccaaca tcatcttgga
     1201 ctttctacat gttactaatg agcgtgtaaa agaacgagat ggtgttttta tttctatcaa
     1261 tgaagctctt tttctttgca atcctcactt caatcctcta taatgtcgca tatagtttag
     1321 taaatgagta aactactaat ttacttattt actaaaaata aaaggatgag gagttaaaat
     1381 aatggaaata ccaacaaatt taaaacataa accagtaatc gttgtagaag actatgataa
     1441 agttgatgga agaaatgcat taaatacgga tgcaaaaggg ctttcactgg gccttgctca
     1501 gtggaatgat cgtggaaaag tcgatatctc aggcaaggta tggcgacata ctggagaaaa
     1561 atggtcacga cagtcggaag aattaccaat cacacgtatt ttagatttag cgatcttagc
     1621 tgcacaagga agtttatact tccaagatgc ttatcgttat gaaaaatttt atgatccaga
     1681 aaatccagtc gtagacatca ttggattaca agggaaccga atgacggtag aagtagatac
     1741 caaaaatcca aagatcgatg aagacatcat tttgtattac gatacactac aaaaagacgg
     1801 agaactgatt ggggaacgat tccgtatttt aaaacgtctt ttagatgagc taggatatta
     1861 atagacagag atgatcaaaa ggggaagaga agcatggaga atttctcact aacagaaaac
     1921 cgaacctttt cacggctaga aaagcaaatg atctggaaga aaccgaaaag ccatcggaca
     1981 agtgcagaag aatggcggat cgcctccgaa atcaaaggga attggcaaga tcctgaaatg
     2041 aaaatcagta acgtactttt ggaaggagat gccgggtcgg ggaagacaca attagccaaa
     2101 gcactatctg ctgatctgca acttccctat acaaaagtga cctgttttgc agatatggac
     2161 aaatcagatg ttttcggagc cttattacct gtagtagatt ctaataagga acaagaccaa
     2221 gaattactag aagctattta ccaaacagat acacttgaag ctgtactgac actgatccag
     2281 aaccattacg gcattgatcc tttttctgct aaagaaaaat tagcagagct agttcaaagg
     2341 atcgaagctg attatccaga tgcaatccat tacagctatt acccttctga aatcgttcga
     2401 gcgattgaaa aaggatattt attagagatt caagagccga ctgtcatccg agatgcctct
     2461 gttcttgtgg cacttaactc agcgctcgaa ccaaatggga tgttgaattt accgacagga
     2521 atcattcgtc gccatccaga ttgcgtcatt gtgatcacta caaatcgaaa ttaccaagga
     2581 aatcgcccat taaatgaatc tttaagagat cggatgcaac atgcggaaaa aatggatctt
     2641 cctgccttag aagtcatggc agaacgtgcg atggcgaaaa ctatgatcca aaaacaagaa
     2701 cttctcttaa agatggcaga gattatccgt ttgttggatg aaactgcaaa agctaacgcc
     2761 atcaaaggtg tagcaggaat gcgttcttat ttttattggg caaataccta taagcaagga
     2821 caagatttac tccagtcgat ttatccaaaa gttttgtata aattatccac tgattcagat
     2881 gaattggcaa ttcttgaaca agctttgtct gacagtggat tgctactgga acttcaggac
     2941 atattgagaa aagaaaaatg gggtcatcct caatctgagc atacaaaagg aagaaccatt
     3001 agcgccgaag aagcaaatga acgaaacatc actgaaaatg ctgaagaaat tctgcgctta
     3061 gatgaaaaga aatcgttgtc tgctgaagaa ccaaatgagg gagaagagaa acaagaagaa
     3121 acaatagaaa aaaacgagat tcctcctcaa atgcaaaaag aacgcagtag cgatatcgaa
     3181 ggaaaaaatc aaaaagaaca acaaacagga gatgattctc aagaaatgtc tgcttctgac
     3241 atggaagcac atgataagga aatcaagaag caactgaata aagaagcgcg acaaatcatg
     3301 aaggaaacga ttcatgaaaa agaaggtctc atcgttcacc gaccagcttt ccaaggaaaa
     3361 acgccggaag cacgtgagct acacatgcaa gtaatgccaa tagtagaatc acttagccgg
     3421 caaattttag agttactgga taatgaacaa acagagacgt accaaaaagg aaaatacgaa
     3481 ggtcagcggt tcaatgcaag caaagtagca tatggtgatt tgagaaattt tgacaaaaag
     3541 aacccgccac atgagcaacc ttctttagcg gtagctgtac ggatcgatga atctgggtca
     3601 atgatacggg atgatcggat cgaagcggca aaaaaagcag cgattgccat tgccgaattt
     3661 tctaagaaag tcgatatccc attgttgatt tacggagata cagcagaccg ttcgtcaaga
     3721 gaaaagactt ctttgttttc ttataaagaa tttgaagacg gatttcaatg gttaaatgaa
     3781 aaattagtca ctatgaaacc aaggcaaaac aatcgtgatg gtgctgcact tcgtttagca
     3841 gcagaaaaat taaacagaca atccgcaaca accaaactgt tgcttaatat tagtgacggt
     3901 cagccaaaag cgttgccgga ttatacaggc aaaaaagcaa aagaagatat ccagtctgtt
     3961 ttgaaagaat atgaacgtca aggaatccta tttgtctccg cagctattgg ccaagacaaa
     4021 gaagaaatca agtcgatcta cggtgaaagt cgttttgtag atattactga cttgaatgaa
     4081 tttcccaagc aaatgattca actgattgcc cgctatctat gataaaataa agctcaacga
     4141 acgcacttag cagattcgtt gagcttttcg tttctagtta ccggtaacaa ggatacttct
     4201 aaaatagaat cggttataat caagagggac cgttaaagga gtgaaacgat atgatacaag
     4261 aacaaaaatg gtggaaagaa gcagtaggct atcaaatcta tcctgctagt ttcaaagata
     4321 gcaatggcga tggtcaagga gacatcaatg ggatccgtga aaaacttaat tatttgaaag
     4381 atcttggtat cggctttatc tggatcaatc cgatctatca atctccattt gtcgataatg
     4441 gttatgacgt aagtgactat caagatatag aagagagatt cggaacgatg gaagattttg
     4501 atttattgct aaaagaagca catgaattag ggatcaaagt gattatggat cttgtcatca
     4561 accatagttc ggatcagcat caatggtttg aagaatcgaa aaaatctaaa gaaagcccgt
     4621 atcgcgatta ttacatttgg gtcgacgggg tagatggcaa agaaccgaat aattggacat
     4681 cgattttcgg cggttctgct tgggagtatt ctcatgaaac tggacagtat tatctgcatg
     4741 tttttgcaaa agaacaacca gacttaaatt gggaaagcga gaaattgaaa gaagaactat
     4801 tcaatatgat ccgatggtgg ttggataaag ggatagatgg atttcgttta gatgcgattt
     4861 ctcatgtaaa aaaagatgaa tattcagtaa aagccacaga gaaccctttc tcaccctttc
     4921 aaaatgtctc aggaatcgaa gaacatttga ccgatttgaa acatgttttt gaagagtacg
     4981 atatcatgac tgtcggagaa gcaagtggtg tcactgcaga agaaggacca caatgggtag
     5041 ggaaagacgg ttatttcgat atgatattcg aatttgatca tatccatatc tggcagcaag
     5101 aaaaagaagg gcaattagat gtccttaaat tgaaacttgc attaagcgct tggcaaactt
     5161 cattggatgg tatcggatgg aacgctttat atatggaaaa ccatgatgtt cctcgtgccg
     5221 tttcagtgtt tggagatact cgtcccgatt tttgggctat gtctgcaaaa gcaatcgcta
     5281 tgatgtattt ctttttgcaa ggtacgccat ttatttatca aggacaggaa atcggaatga
     5341 cgaatatgcc ctttgaatcg atcgatcaag ttgatgcagt agattcgaaa agactttata
     5401 aaaggctgct tgcggaaggt aagacaagag aagaagcatt ggatatcatt cgtgaaacaa
     5461 cgagagacaa cagcaggaca cccatgcaat ggacttcgga acaatatgct ggtttttcta
     5521 cacatgaacc ttggctgatc actaatccaa ataccaaaac gatcaatgta gaacaacaag
     5581 aatatgaacc agagtcggtc ctgcaattct ataagaacat gatccgcatt agacaaacca
     5641 ataaaggtct gatatacggt tcttataaag aatatcttca tgaacatcca caactttatg
     5701 tctatgaaag atatttagaa gatgaacaat atcttattat ggtaaactta acagaatcac
     5761 ttgctgacta tgaacttcca aaagaagcag atcagtcctg gacattactc ttgtcaaatt
     5821 cctctagcgg agaatttgaa gctaaaggca tactagcacc ttatgaagct cggttatata
     5881 aaacaaacaa gtaaaacttc ctcgttaaag ttcattagtt ggactttgcg aggaagtttt
     5941 tcagattgct ctgtcaatta ctcatttcaa taattcgctc taggaccacc gatatattta
     6001 ggacgagagc gaagggctgt cacgtgagct tgtccaaggg ttttcaaacg aggtctgaca
     6061 ggtacttgga catgtttgat atgcataccg attgatgtgt caccgatatc gataccggct
     6121 tgtccaacta cgtgttccac ttcgacagga tcattgaact ggtcaaaagc agcaacagaa
     6181 caagcacctc cagcatgtag tgcaggaaca acagagacaa tttcgaaatt ttttgcttct
     6241 gccactgttc gttctactac taatgctcgg ttcaaatgtt cacaaccttg gacagctagc
     6301 gcaatttctt taggatccaa caactctttt aacgtacgga tgatccattg accaacttcc
     6361 gcactggaat tttttccaat atgtcctcca acgacttcgc ttgtgctgca tcctaaaaca
     6421 aaaagatcac cttttttcaa gtgtgcctca gccaatatgt cattaactat ttcagtcaat
     6481 tggtctttta attctttttc tgacaacttc attttctaaa accttctttt tcaattaata
     6541 ttgcttccgt gcttgaatac gttgaagact tgcagttata ggaaaggcaa gtcccattcc
     6601 aatcagattt tggaagatgt ttcctggtat agaagccact ccagccggcc aatcatataa
     6661 aatccaaccg gcgataccat agccgatgat cataactaag cttcctaata aaagccctaa
     6721 tccccacata cgagtagaag atttgctgaa agaaccagca acatatcctt gtaacccgtg
     6781 gatcactaat gaaaaaactg cccactgcgg atatcctgaa atcaaatcga tcaatagacc
     6841 actggaagca ccaacaacta atccacccgt tggaccataa agaaaagcag cggtatagat
     6901 cccggcctcg cataatgtca caaatccatt cgtagccggt acagggatca aaattgttaa
     6961 tgaaagggca actgtcaacg caatcagcat agcatagatc gttaattgct tagttttcat
     7021 ttctcattcc tcctttttga ataccgccag accagacact tccggtatga tcgttcatca
     7081 gcacgccatt agaaatacaa tgggcaacaa attttttact ttctgctact gctttggtaa
     7141 aatctgcccc taacacaaga ttcgctgtta ttgcagaaga aaaggcacaa ccggccccgt
     7201 ttactgttgt agtgtcgatt tttttatgag aaaatacctc ataatgatca attgaataga
     7261 gaagatccac agcttcatct ccagccatcc ttgtgccgcc tttgatcaca atgttttttg
     7321 ttcctgaagc agtgagttct ttagcgagat ggatcatttc tgttaacgaa gagggaagag
     7381 cttgaccaga aagtaaagca gcttctgcca aattaggcgt aatcatcgta acaagtggaa
     7441 aaagttcttc gatgagctgc gaaatataca attgattgat cacagcttca gtttctttga
     7501 atgccaaaac agggtctaaa atgattggga tattttgttt tgtttttatg aagtctcgaa
     7561 caaccatgat tgcttcactt gaattcaata acccaatttt gataccatct aatgaagtca
     7621 ttttatcaat cgaaactaac tgtcgtttaa gcaattccaa agatatagat tccagcataa
     7681 atcgctcatt aatatctagt gcccctatag aagtcaaggc gcttagtccg aatagaccaa
     7741 aattctcaaa agtttttaaa tctgtttgga ttcctccacc agcaaatgga tcagaaccgc
     7801 caattgtcaa gattcttttc ataacatttt cctccatgaa taagagtata acgaaatttt
     7861 caaaaaagaa aaacagccaa ttggcatatt tttatctcat ccaatttaaa aaaacaaaaa
     7921 gaaaagaaag accaaaatgg atctttcttt ccttaaatag ttaggacttt tgtgatttta
     7981 attctttctc gatttgaagc cgttgcactt tcattgtagc tgttcgcggg attgcatcaa
     8041 aatcacggat gatagggacg ttcaagcgag gaagttcatg gacttgctcc caccatcgat
     8101 tccaatccat ttctttatca ggagctaaag caataatcgg ttgtggagaa tgatttttat
     8161 cccttacaat cactacttct gccaaaaaat ctaacgaatc taataaaaag tcttcaatag
     8221 ctaatgtact attgatcgtt tcgatcaaat ccacttgccg atctttcaaa aataggtggc
     8281 catgcttatc cataaaaccg taatctccgc tatcccacca atcgccataa acattttcct
     8341 ggaaccgtgc atcttccttg taataagtca aagcccgtcc tttagacagt aattgtatat
     8401 gaccatctgt gttttcaggt aggacattcc caactgaatc tgtaatccga gcgcttgtca
     8461 tgtcttccag tccaacgccc atatctcttg catcacttgt tttcaaactc tctagtgtat
     8521 gagccttaag aatcattggt ccgcattcac tttggccata tacttgaaga aataccgcat
     8581 cctgtgccat cgacgttcgc aaaaatgtct tcattgtctg gttgtttata gcatcaaaag
     8641 tggagtgata gtatcggata cccgaaaaag cttctggatg ctccttagca gtaaaacgcc
     8701 actgaacaaa gttattcgga tgggtctcta gtgcaattgg ccggtgagct tccaacatat
     8761 gaacaacttt tgaactttgt gcgtttgcta aaggcatcat aggaaatccc atggacatca
     8821 aagaagagat accaatattg aatctggaat gcactggaga gatgtgaaaa gcgattaatt
     8881 tcttttccga aatttttgtg aaaactgttt tttgccattt cgtccgccat cccatagaat
     8941 gagcagaatg acaaataagt ttagggatac cagttgtacc agaagtatgg gtcatataag
     9001 aaatttcatt tttgtctaaa aatgattgag aaacagacaa agcaggttgt tccattaaat
     9061 aaagaatagg
//
LOCUS       DACEXQ010000103         8992 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.103, whole genome shotgun sequence.
ACCESSION   DACEXQ010000103 DACEXQ010000000
VERSION     DACEXQ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.103"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..389)
                     /locus_tag="IXH04_001663"
     CDS             complement(<1..389)
                     /locus_tag="IXH04_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAQ1852266.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRA"
     gene            complement(521..1525)
                     /locus_tag="IXH04_001664"
     CDS             complement(521..1525)
                     /locus_tag="IXH04_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ1852267.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(1522..2511)
                     /locus_tag="IXH04_001665"
     CDS             complement(1522..2511)
                     /locus_tag="IXH04_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ1852268.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(2508..3314)
                     /locus_tag="IXH04_001666"
     CDS             complement(2508..3314)
                     /locus_tag="IXH04_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1852269.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            3480..4436
                     /locus_tag="IXH04_001667"
     CDS             3480..4436
                     /locus_tag="IXH04_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1852270.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVRKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(4513..5031)
                     /locus_tag="IXH04_001668"
     CDS             complement(4513..5031)
                     /locus_tag="IXH04_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1852271.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(5119..5268)
                     /gene="rpmG"
                     /locus_tag="IXH04_001669"
     CDS             complement(5119..5268)
                     /gene="rpmG"
                     /locus_tag="IXH04_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ1852272.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            5496..5942
                     /locus_tag="IXH04_001670"
     CDS             5496..5942
                     /locus_tag="IXH04_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ1852273.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(6137..8032)
                     /gene="asnB"
                     /locus_tag="IXH04_001671"
     CDS             complement(6137..8032)
                     /gene="asnB"
                     /locus_tag="IXH04_001671"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAQ1852274.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTILSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(8357..>8992)
                     /locus_tag="IXH04_001672"
     CDS             complement(8357..>8992)
                     /locus_tag="IXH04_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HAQ1852275.1"
                     /translation="EAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDG
                     LHLYDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLP
                     GDLSSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIY
                     SSCCNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT         2889 a         1838 c         1551 g         2714 t
ORIGIN      
        1 gctctattgt agataatacc tggtacaatg aggagatcga tcatctccgg caaatagagc
       61 ttctctgatt gtggctcaag aacaccaaaa ctcgaacgta caagaacgga ctgttcatcc
      121 atttcataaa acgccatctg acgtttcggc aaagttttag gtacaactat tttcttttgt
      181 tctttttctg ctcgttggat aaccggctgt gtatccagtt caaattcatt cgataaagtc
      241 gtccctatca cttttgctga cttccagtac tcgctggcga atagctgttt atagattcgc
      301 tgttcttttt cctttttctt gccacttttg gccagatttt tcagcttttc gattcctttc
      361 cggcgtaatc ttactttcaa ttggtccaaa aactcaccct tccttctctc ataaaatggt
      421 ataggaaaaa aagaaaaata gcaatcgaac tttcaaataa atgcataaat agaataaaaa
      481 gaagacctag aaatgattgg ccttcctttt ttaatgaatt tcaactgttc agcgcgtgtt
      541 tttgtctgat aaaaagtgca tacagaaaat atggcgcacc aaaaagggca atcaaaattc
      601 ctgtaggtac ttccccactt agtagaatca ctctagcaac catatctgca ctaagcagaa
      661 aaatggaacc gaacaaaccg ctcagaacaa aggtccagtt attttcttta cgtacgacta
      721 gcttcgcagc atgaggagct agtagtccta caaaagataa actacctgtc accgctacac
      781 taccgcttgc caaagcaaca gctaacaaca aaaggatttt ttgactttta gccacgttaa
      841 ggcctaatcc ttgcgcgcgt tcttgaccaa atgatagcaa tacaatcctc tgtgcataaa
      901 gtcctcctag cggaattaag ataaccgccc atggaagcaa tgcccaaaca taaggccagc
      961 tcgcacccca gattgatccc gctaaccatg cattcacaaa tccataattt tccttagata
     1021 attggatcgt gcatagcaat gttaatgcag aaaggccggc attaacggca ataccggaca
     1081 ataagattcg attcataccc aaaaagccat ttcgatcata ggccgcaaga tagacacagc
     1141 aagcagctaa aacaccacct agaaaggcta caactagcaa tagaaacggc agcgaatagt
     1201 gcgaaaagaa acctaaataa agcatcacta aaaatcctgc tccggcatta atgcccaata
     1261 tactagaatc tgccaattca tttcttgtta ctccttgcaa gatataacca gaaacgccaa
     1321 gacaagctcc tgctaataaa ctgatcagta atcgaggaac tcgaaattct ctaacaatca
     1381 gcgccaaaga agatgattct ttccctatga acgtggcaaa aagctgatcc aggtaaataa
     1441 aaggcgtacc aatcattaaa ctacctatag acaggatgac aagtacaaaa agcaagaaat
     1501 agaacgtcca atatattttt ttcaccgttg ttccctcctt acttgtatca gtaaaaacgg
     1561 aacgccaatc aatgcaatga ctaatccaaa aggtgtttca aaagggggat tgatcatcct
     1621 cgctgtaaaa tccgccaaca taaccaatcc accgccaact aaaaaagaga caggtagaac
     1681 ataacgatag tccgttccaa ccattccgcg aacaactggc ggaaccaaca gcccaataaa
     1741 tgaaacaggt ccgactaacg aaacggcaat ggctgctaac actgcaacta gacagaaaac
     1801 aatcaaccgc gttcgtttag gatgacttcc aagagctaat gcatgtgttt cactcaaatg
     1861 aatcaaagat attgcttttc ctaatgaaaa agaaaccaaa acagcaagta tatataaagg
     1921 aaacaatact gctaattgtt tccatgtgat attggcagct ccgcctacaa accaaaaagc
     1981 aagatcctgc tgcagattaa agaattgggt aagtgcttgg ctgatagcag taaaaaaaga
     2041 actaatagct acgccagcta aaatcagctg taaaggattc atgcccattt tcgaaaactg
     2101 caaaaaacca aatacgagca aagttacagc aaaagaacct gccaatgaca ttagaaacac
     2161 agaaaaatca gaagctttag gccaaaaagc aaaactaagg gccattgcca acgaagcacc
     2221 cgcgttgatt cctagtaagc ctgaatctgc taaaggattt cttgtcactc cttgcatcaa
     2281 tgcaccgctc aatgcaaaac aactccctac aagaaatgct cccgccacac gaggcaaacg
     2341 aatatttcgt atgatttggt gactttgttt tgatctatca aaatcaaaca acgcctgcca
     2401 aaaatctgct tttcccacag tcgctgcgcc atttgaaaga gcaacaaaaa aaataatcaa
     2461 aagaacaaaa cagaccaaaa gcaggagtct atttttcttc atagtcctca taattgatcc
     2521 tcctgctctt ttggcaacaa atcataggtt agaaaaactg gtttatttgt tcttggaagc
     2581 gtgacaatcg ttgcatcaat ctcaaatatt tttttcatac actcttttgt gatcacttct
     2641 tcaggtgtac cttctgtgat caacttaccg tttttcattg ctaataaata atctgaaaaa
     2701 cgtgaagcat gattaatgtc atgaatcgac atcaagatcg ttgtttgttt catttgattg
     2761 atttcagtca ataactgtag gatctctaat tggtgtgccg gatctaaaaa tgttgtcggt
     2821 tcatccaaga tcaagatatc tgtttcctga gcaagcgcca tagcgatcca gacacgctgg
     2881 gtttgtccac cagacagcgt tgcgatatcc cgcatacgaa atccagctag acctgtagca
     2941 tccaacgccc actcaattgc ttcatgatct tgcgtgtcca tgccactgaa cagtttttga
     3001 taagggtatc gtccgtaaga tactacttct tctactgtca tacctgtagg gtgttcacta
     3061 gtttgtgcta ataatgcaat tttttttgca agttcttttg aactgtatgt agagatatca
     3121 gagttattga caatgatctt tcctgatttc acaggtagta tacgggtaaa acttttcaat
     3181 aaagtagatt ttccacttcc atttggccca atcaaactag tgattttccc ttcaggaatc
     3241 gtaacagaca tgttttcaat gatttttttg ttttcgtagc cggctgcaat gtcagctgct
     3301 cttaatctcg tcatttcacc catcctttcg ctgaaaatca ttctcaatta tgacgctgtt
     3361 tcaacatttc gtcaagcgct actttcatat ctatagccaa atgaagcggt tgacagcgtt
     3421 cgaaatatca ggaataataa ccaatgagaa agattctcaa ttatgttagg aggaaagaaa
     3481 tgaaattaaa aacaaaagta ttcacattat ctagcatatt gctgctggct ggagtactga
     3541 gcgcttgctc cccctcttca gataaagaaa cagctacaac tgccaattcg acgacttcat
     3601 ccaatagtac tcatacagtc acagatacac ttggacataa agtagagatt ccaaacgcgc
     3661 ccaaacggat catcggcagt tacttagaag actatctgat tgcactaggg gaaaaaccta
     3721 tcgcccagtg gactgttggc agtggttcca ttcaagatta tctgcaagat gatttaaagg
     3781 atgtcccaac gatttcttat gaccttcctt atgagaaagt gttaagcttt gagcctgatt
     3841 tgctgttgat ttcttcttcc gctactgtcg aaggcgggaa atacgaacaa tatagcaaaa
     3901 tcgcgccaac ttatgttgta aaaaacggaa cagatgtcac atgggaagaa caactaaaag
     3961 atgtcggcaa agctttggca aaagaaaaag aggcagaaaa aatcatttcc gattatcaaa
     4021 aatccatcaa gaaaactcga gaagaactag cagataaaat cgagaacaag acagctgcag
     4081 tgttgtgggt gaccaacaac tcagccttta tggtcagaaa aaatcggtca agtggaagaa
     4141 tcgtatacga cgatctggaa tttggtgttc ctgatctagt ggaagaggtc tccaaaaaag
     4201 ccacttctga ctggtctgct gtttcctctg aaaagctagc tgaattagat gctgactaca
     4261 tcatccttgt gaacagtgac aaagatgctg ccatgttcaa tgaaccaagc tggaagaatc
     4321 taaaagcagt gacagacaat catgtactgc aattcggacc tgaatctagt tggctatata
     4381 acggacctat tgccagtcag gaaatggtca atgatatcaa agaaaaacta aattgatttt
     4441 aactaaaaaa agcatcttca cgattagtcg tgagatgctt tttttcttat taacaaacta
     4501 gacgcataga ttttattctt cttttgaagc aaccctattt tttactcttt cccatttttg
     4561 aaaacgatgg gcatatagat tttcttgatc cgttatcccc tcatactcct ctataagggc
     4621 gaagtctgaa aaatcaatat tcccaatata cgtatcgcct tcaaacgtgc tatcgatcaa
     4681 tgttcgccag atccgaccag tgtaaggaag aagttcttga aaaatccgac taccacccga
     4741 aacatagata tcttctgaga tcgtttgact atacgacaaa agttcatcga tcgaatgcat
     4801 tacttccgca ttttctttcg tgcgaaaatt tggttgcgtt gtcaacacga tgatatggcg
     4861 ataaggaagt gaaagatccc ccattccttc ataggtcttt cttcccatca ctaaaattcg
     4921 atccattgtg tgttcgcgaa agaaacgcat atcatttggc aatcgccatg gtaacaaacc
     4981 atttttgccg atcagaccat ttttatcttg tgcccacatg gaaataaaca tattatccgc
     5041 tccttatgat tttagatgtt ttgagataaa aaaagtctca attctttgac cagcaaggaa
     5101 ttgagaactt taaaaacatt attttgtttc acggtgcaaa gtaactttgc gttcgcgtgg
     5161 gcaatatttt tgtttttcca aacgatcagg attgttacgt ttatttttgc ttgttagata
     5221 gttacgttct ttacaagaag tacactctaa agtgatgttt acgcgcatgt ttttccctcc
     5281 catttttaca agaatcctct aagatgactc tcagacttga ttatcttacc atgatttttt
     5341 ctagattgct agtgtttttc acgattttgt taagtaaaac tacttgacga taaatacaac
     5401 ggttttactt ctcaaacaaa atctttttct ttttgtataa atacccttac acttgtttat
     5461 atagattatg ttttttaata aagggggata ctaatatgcc atggaacatg aaggactttc
     5521 ctgcatcaat gaaaaactta gataaattga caagaaaaaa agcaatcgac atcgcaaatg
     5581 cattattaga tgaagggtac cctgataacc gtgcgatccc aattgccatt gatcaagcaa
     5641 aaaaatggta tgaagatgct agtgaatcag aaaaacgaac tttcgaaaaa gaaaaaaatc
     5701 caagcaagac ggatgaacac gatacgaatc ctcgtgctgg aaaactgctt gattcagatg
     5761 tgatcgtaga atatgaggaa gaacaatgga tcgtgaaatc taaaggggca aaaaaggcaa
     5821 gcaaccattt tgatacaaaa aaagaagcaa tcgaaaaagg aaagcaagta gcacaaaaca
     5881 aagaatcaac tttgttcatt tataaaaaag atgggacaaa agaaaaagaa atcgattatt
     5941 gaacatatgt aaaggggttg tgacgaaaat cgtgtcacaa cccccttatg ttcgaaacaa
     6001 tggtgttcct ctgccaactc cgaaccataa gtcataatat ctataaaacg ggaagagctg
     6061 ttttttgaaa ttgtttctta tcactcagtg ttaaacggta ggagaaaacc tcttttttat
     6121 tgatatttga ttttcatcaa acagtcacat agtcgattgc ttctgcttgc ggaatcgttt
     6181 catctacaaa gtagacttta taccatgtca agaaactgag gattgtccag atttttcttt
     6241 gctcttcttt acgtccttcg tgatggtcat ctagcaactt catgattttt tcttgatcaa
     6301 aaaattcttt tgcaaaatct gcttcgaata atgctttgac ttgttcgtag ccgtgttctt
     6361 ctttcaacca agctttgatt ggcacaggga atcctaattt cacacgattc gaccattctt
     6421 ccggaagatg tttgttcgca gcttgtctga aaatatcttt tgtgttgttt tgattcaata
     6481 aaaacttact tggtatttgt tgagccaatt tcatgacttc gatatccaat aaaggtactc
     6541 ggacttccaa agaacttgcc atagaaagtt tatctgcctt taacaagata tcttttggca
     6601 tccattgatg aagatcgaca tattgcatct tattgacttc atctttgatt ccttctgttt
     6661 tctcgtaatg aacagtcatg atttcatcca cagatggtgc tgtttgatat tttggtgtta
     6721 acacttctga tgcttctgat tcttcgaaca ctcgagcatg accgataaaa tattcttttg
     6781 caggagcaag tgattcatat aaatggatcc gtccatggaa atttttcatt tttccgattt
     6841 tacgtcctaa attatagcgt gttccttttg gtaatttttt caatccttga gcaatcacac
     6901 ggatgaattt tgaattcgta tggaatccat acgtttggta tcccgcaaac agttcatccg
     6961 ccccttctcc agattggact acccgcacac tttgtgaagc aagatttgct aaaaagtaca
     7021 aaggtacgca agaaggattt gaatcaggtt catctaagtg atactggatc aaagggaatg
     7081 ctttgaacga catgtctccg tcgatcactg ctgctgtatt atctaagtcc aatagttcgg
     7141 ttagttttct ggcttcaatc gcttcattat aggttttatc atcaaacccg attgagaaag
     7201 aatgatctgg tttcaataca gaggttacat aactggaatc aaccccgctg gataagaaag
     7261 aaccaacttc tacatcactg accgtatgtg cttcaataga agcttgaacg gtttcgtcga
     7321 ttttttcgat ccattcttgt cgggaatgcg tttcttttgt ttcaaaatct gcatcccaat
     7381 attgctgaat gtctattttt ccatctttat acgtataata atggccttca ggcaaacgat
     7441 agacatcttt gaagaatgtt tcgccgttta atggagaata ttggaaagtc atataaggtt
     7501 tcaacgcttc tttgttcaat tctttgttaa aatcaggatg aggcagaaaa cttttgattt
     7561 ctgaaccaaa cataaatgtt ccgttcattt ctgcataata atatggtttg atcccaaagt
     7621 ggtctcttgc accgaataac tcattctttt cattatccca aatagcaaaa gcaaacattc
     7681 cgcggatctt ttgcaataat tctgttcccc attcttcgta accatgcaaa aggacttctg
     7741 tatctgcatg tgtttgaaaa acgtggccag ctgcaatcaa ttcttctctc aaaggctggt
     7801 aattatagat ttcgccatta aaaataatga tctttgtccg atcttcattg taaataggct
     7861 gagtaccgcc ctctaagtca ataatactta atcttctgaa tcctaaagca acatgtttat
     7921 cgatatattc tcctgaacta ttaggtccac ggtgaacgat tcgatccatc attgtattaa
     7981 taattgtttt tttgttgtcc ttgtcgttaa caaaacctac aataccgcac atttaacttt
     8041 cacatcctaa tataaaatta ctgtttattt cattcatatg catcttatca tagaataaat
     8101 agcggacgaa tattttctct cttacaattt cgtcacgtga aaacaaaaga tgaaagtttc
     8161 tgtttttaca gaaaataaag tcccaatatc taatttacca tgttttcttt tttgaggcta
     8221 ccttattctc ttcgtgttaa aaaaatatta gtggatgcta tttccaaaaa aaccagcaaa
     8281 aaaagacacg acctaaagat cgtgtctaat tatgactgta tcagaaaata tgataccaaa
     8341 cggatctatt cattaattaa ttgacatagt ttatctgttg ctcttttatg actggataac
     8401 tgaccaattc atgacgatcc aagtcttctt tattcatatt tacagcagta atttgactgt
     8461 cttcatacgt acgataataa taaatccctt tatcaacgtt acagcaagaa gaataaatcg
     8521 tatattcgta ttttctttct ccagtatcgc atactccttt ttgttgctcg actgagccta
     8581 gtatatgaaa aaattgactg atactttcag attctgaatc acctgatgca gcattcattt
     8641 tagtaaatgt ggcttttaca aatcgagaaa cagaagagag atctccaggt aagcccaatc
     8701 ctcccatccc acgactataa acattcaagc tgatctggtt tgaaaaatga ttttctggtg
     8761 ttccatttga tagcgagcga taattgttca gattaaataa ttgataatca aatggcggat
     8821 tatttgtcaa cacaccgaca ggattatcat ataaatgaag tccatctttc atactttcaa
     8881 ttactatcga tttttcttta tcagctaata accagtgtaa aggtgacaag ggtagttttt
     8941 cactaaaatc gatattgact agattgatag tagccaataa ttctttagct tc
//
LOCUS       DACEXQ010000104         8869 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.104, whole genome shotgun sequence.
ACCESSION   DACEXQ010000104 DACEXQ010000000
VERSION     DACEXQ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8869
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.104"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..401)
                     /locus_tag="IXH04_001673"
     CDS             complement(<1..401)
                     /locus_tag="IXH04_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ1852276.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQT"
     gene            complement(1273..2904)
                     /locus_tag="IXH04_001674"
     CDS             complement(1273..2904)
                     /locus_tag="IXH04_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAQ1852277.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(2918..4591)
                     /locus_tag="IXH04_001675"
     CDS             complement(2918..4591)
                     /locus_tag="IXH04_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1852278.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(4604..4921)
                     /locus_tag="IXH04_001676"
     CDS             complement(4604..4921)
                     /locus_tag="IXH04_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ1852279.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(4946..6346)
                     /gene="lacG"
                     /locus_tag="IXH04_001677"
     CDS             complement(4946..6346)
                     /gene="lacG"
                     /locus_tag="IXH04_001677"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAQ1852280.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            6583..7368
                     /locus_tag="IXH04_001678"
     CDS             6583..7368
                     /locus_tag="IXH04_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ1852281.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            7371..8063
                     /locus_tag="IXH04_001679"
     CDS             7371..8063
                     /locus_tag="IXH04_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1852282.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(8337..8708)
                     /locus_tag="IXH04_001680"
     CDS             complement(8337..8708)
                     /locus_tag="IXH04_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAQ1852283.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
BASE COUNT         2691 a         1937 c         1539 g         2702 t
ORIGIN      
        1 atggtttggg gcgtgtagta atgcccatac attttctcaa tcaagtctgc gatttccgac
       61 atggtaatac cttttcggaa gaggtgaatg acggtctcct ctaacgtgtc attcgtccgt
      121 ctataagcag gaacagtctg ttgcttgaac tcaccattgc ggtcgcgcgg aatctggaga
      181 tgaagttccc catactcggt cttgaccgta cggtcatagg agccgttacg agaattaccc
      241 gtgttaaaac caatgcgatc atacttttcg taatctagga aagccgttaa ctccgttttg
      301 agaagcgtat tgactgcttt ctccaaatgg acacggaata attcattcaa atcgcctttg
      361 ttcgctagag tctttagaat ttctgtagta aaatcgttca tagggaagtc ctctcttctg
      421 tgaatgtgtt gtggtaactc tattctacag aagggacttc cttttttcta tttacacaaa
      481 atattttaca ctctccatta taacaattgg gttaaaaaga aaatagatag tattctacct
      541 catatttatt tgaaagtaat atataaaaga cgttcaaatt cgaacgcctc tcgttaacca
      601 aatattggat tcagaatcta gttttcatat ataaacagag tatgattagg tgcaaaaatt
      661 gcacttttat tgcactttta tttttattta tagtgtttta ctgttctgta tattgtttta
      721 actcccttga tagtatcgca ttctgtttta tacactttac gttgaaaaca gagtgggaat
      781 aaagagccaa cataatatgg agggggaatc cctataaatc aacattcttc aatagctaaa
      841 atgcttgatt ttcattaaaa ataagatggt tccctaccaa aagagactat aatataagtt
      901 aatttgtttc attcttatca taaaaagaag ttctcgaaaa acgaactgtt ttccgagaac
      961 ttttttctat ccatctaagt gctcaaatca tattcacgac ggaattctaa ggttaccctt
     1021 tcttaaacaa actttatgtt atctgcacaa ctttttgcaa ttttttcatt gcttcttacg
     1081 aaatcgttga actctcttta gcataataca aagtgtagat gaagtctggt gctttactcg
     1141 ttttggtaaa aatgactgga aatcgtttct ggttattttt ggcaaaggcg aggtgcaggt
     1201 tcgattcctg ccttctacgc taacgatgtg gctgaaggga acctccgatc ttgaatcgaa
     1261 acgatccgca gtctattgtt tcaccaatga aacaaaatca acgttcgtcc aagcatttcc
     1321 tgaagccttg ctataaaccc caatttcaca aacacctgtt gtaattggaa tgtctttgat
     1381 tgtaattttt gtccattcac ttgttttaac aaccgcagta tttagttccg ctccaccata
     1441 atttttgata tataactgat tagtatcaaa ctctccccca gacattacat aggcactcaa
     1501 tgtgtaagtt cctttgctta tgccactaac attttgataa gctgatgcct cgtatgcttg
     1561 atcctgccaa aaagttagtt tgtagtcccc aaaaaaagtg cccgcttccg ttttgatcgt
     1621 acccttagcg gttccttgtt gaaaccaggt ttgccaattg gaaggactgg tcgttggtcc
     1681 atcgctttcg aaacttgcat ttttaactag atttatttgc gcccctgctt gtttaaacgc
     1741 acgcaagctt ggcaaggcgt ttccagcaaa atcaaacatc gcttggttgt cccattcgtt
     1801 tcccatagtg gattgaacac tgagataatt catacctgct tgtgttgccc aactcacatt
     1861 tccgttgtac caaagaggtt cccaatagaa gaagcccgcc ccgcgattat tcggtactgc
     1921 ctgaatccga tcggcaagat ccagcaaata ctctgcttgt cctgtcggtg ttggttcata
     1981 tccccccgca gctgcttcgg cttcaccaaa agcatttggc atagtatcgg cattcgctgt
     2041 tgtatgccca taagctgttt caactacgat cacttcttta ccataccgtt ggctgatcgc
     2101 attcatattg gtttgaagtt cattcattga accgtgccaa tacggataat acgacactcc
     2161 aattaaatca aatgacacac ctcgactcgt gatttcatca aaccaccaag taaatgctcc
     2221 tgtatcgcca ccatcagcta agtggagcat tactggagtt gttttaggtt gagtgtctcg
     2281 aacagcttgg ataccagact tcaaaaaagc agccaatcga tcaaattctc ccccgccttc
     2341 tccccaagac ttgccataag gccagagcat tccgctattc agttcattgc cgatctgaac
     2401 catatcagga taaacgtcca gctgcttcat ttccgacaga acgttctttg tatagtcgta
     2461 caatgccgta ttcatttctt caaaggtcag accttgccat gctttaggaa gattctgttt
     2521 cccaggatca acccaaaaat cactgtagtg aaaatccagc agcaccttaa gtcccagatt
     2581 ttttgctcgt tttgcaaggt caatcgtcgt ttccagatca tttgtccctg ctccataact
     2641 ttctccatta ctattataag gatcctgcca caaacgcagt cgaacatagt tgacaccatt
     2701 attttttaaa atctctaagg catcttttga aatatcgtct tcataatatt ttgcaccaga
     2761 tttttccata tctgacagga tcgaaatatc tgctccgcga ataaattcgc tggcagatgc
     2821 ttcaacagtt tttgaaacag cccccaaagc ccctaacaca gctgcaaata aaacagcttt
     2881 acgcaaccaa attcccattt tcattatggc tcctccttta ttgaaattgg ttcagaataa
     2941 actgaacagc tttttcagga tctctcgtta atccgatata ttctgctcct tttgtcgcaa
     3001 ccagcttgat tcctaagcga tcggtgtctt ctttgatatc atcatagtaa gaattgactt
     3061 gcggtgctaa gaccaccata tcaaaatcct gcagcatttc gtaatgagaa ccataagcac
     3121 ccgccattgc actgatcggt gctttcgatt cagatgcacc tttttttaaa gcattagcta
     3181 acatcgcact tgtccctgcg ccagcacaaa gtaccaacac attgatttct ttatttgcat
     3241 cgctgataac ttgactcgct gccacaggta cttcctcacg tacatcggca gaagcacctt
     3301 cctcggttgc gatcacctct gctttttgtg cttctttctc tactaagaca gcatcgtatg
     3361 ctttgcaaaa tggtagatag atcaggaagt caacgacgat tagaaccgct gctagaacaa
     3421 aagctaagat acccatgccc gttcctagaa ccaaaccgat cggtgctgga gttgcccaag
     3481 gcaaaacgta cataaagctg ttcatgccca atacatcgac aaaaaacttg aacaggcaaa
     3541 cgtttacaac aggtgttaac aaaaatggaa taaaaaaata agggttcaaa atcatgggtg
     3601 ccgcaaatag caatggttcg ttgacagcga aacttactgg aataaaggaa gctttcccaa
     3661 ctgccttcaa ctgcttagac ttagcaaaga gtaggaagat aaaaggaaca accaatgttg
     3721 ccccagttcc tcccatagtt cctacaaaat tccctaaacc caccgtcagt actttagaag
     3781 cttgttcacc ggaattgaac aattgcagat tggtttccac attggcatag ataattgccg
     3841 caattgccgg ctccacgatc gacggtccat gaacaccgac aaaccaaaac agcgccatcg
     3901 ctccccagat aatagcgatt cctaagtacc cgtcagctgc tgtaaatagc ggctgcagca
     3961 ccgtaatgat tgcttctgca aatgagattt ggaagatttg tcgaatgcct aaatcaatta
     4021 aaacggccgc aaatactgaa aaagagaaag gaaaaatatc tcggaaggtt tgcgaaatcg
     4081 ttccaggaac ttctttaggc attttaatgg taatatcttt cagcacacaa aacttatata
     4141 aattaactgt taaaaatgca gcgacaaagg aagccagtag tccctttgtc cccatatatt
     4201 ccgttgaaaa tccaccttca atcggagaga cgcttaacaa caggaaactt accacagagg
     4261 ccagcatcac agaggtggtg ttgattactt tgtttttcgg taagcgacga ttcatcgaat
     4321 cagccaagca gcgagcagtg gttgctgaaa cgagtaaccc tacaacaccc atcgaataat
     4381 tatagacttt ccatagccat gtggagacag attcaggcca ttggtaacca aaaatctcag
     4441 gaaccgcagc aatcaaaatg aaaatgcttg aaaataaaat agctggcatc gctgttaaaa
     4501 atccgtcacg aacggctcct aaataactat tacgtgagat tttttcaaaa aatggtttcc
     4561 ctttttcgat ttgtttaatg attccatcca tagcgttctt ctcctatcct tgattctgtc
     4621 tgtacagtac aataaagtct tgaatcagat ctctcagcaa taaagtggtc attaaatgat
     4681 cctgcccatg gataaaaatg aatcccatct ccatctcttt cccagatgct tcttccgcta
     4741 aaatttttgt ttgcgaatta tgtgcaaggg ttaaattttc gtcggcttct ttcaacgccg
     4801 aatcaacatg ttcaaagttt ccttgtctta cttctttcaa taatttcaat aatgtactgc
     4861 gagcgtcgcc tgaatatgca acgatctcaa aaccaagcat ttgtaattcc tctttattca
     4921 tcttttcttc ctccattatc acttattaaa taatcgtttt ggtttcactg accaacttgt
     4981 accagtaagc tgattctttt gggtaacgct tctgggtttc aaaatcaaca taaaacaagc
     5041 catacctttt gttataccca tttgtccatg agaaaagatc cataagcgac cacaaaaaat
     5101 accccttcac atttaccccg gctgttatcg ccttgcttaa ggattccaaa tagactctca
     5161 aatagtcgat tctcggctga tccataataa tgccatcttc aaactgatct ttatacccca
     5221 tcccgttctc agtgatatag attttgttgt aatgtgggta atcgctttta attcgcagca
     5281 acaagtcata tagaccttct ggataaatca gccagtccca atcagttcga ggaattcctt
     5341 ctttataaat acgttctccg attcccttca ctttataaac agaggtccct ttttcacccg
     5401 taccgttatg atggattgca ttttctccat cgtaagcttt gacaaaatgg cattggtagt
     5461 gattgatccc taagtagtca ttacgcgtgg atgctttttt caattcaacg aaatcttctt
     5521 caggaaaatg atagctggcc tgatttgctt cacatatctc atcaagagca gtcaaggttt
     5581 cagttgaata atagcctaaa taggtagcat ctaataagaa acggatcgac aatgcatcgt
     5641 ctaaaaaggc agcatgctta tcttccggcg cgtctgttgc cgcatatttc gtctctaatg
     5701 agtgaactac accaatttca cccggaagtt cgttttcttt gaaatagttc actacacggg
     5761 catgagcaac catcatatta tgaagacagg cgacgatctt tgtaaaatca tatttgattc
     5821 ctggtgggaa aacacctaac aaatattgat tggttgcaac tggatagatt tcattaaagg
     5881 tactccacac cttgacttct ttgaattcat gaaaacaaaa aatggcatag gaaacaaatg
     5941 cttctattgt ttctcgattc aaaaaatctc catggtcaaa caaacgttta ggggtatcaa
     6001 aatgatgcaa cgtcacaaac ggcgttacgt ttcttttctt acattcttcg aacacacgat
     6061 gataaaaagc gattccttct ggatttggtt tcccagctcc atcaggaaaa atgcggctcc
     6121 aagcaatgga taatcttaga ccatttacac caaaacgttc acacaattca atgtcctttg
     6181 catattgatg ataaaaatca cttgccggat cgggactgaa tcgcccttgt tcctctaaaa
     6241 agtcatccca agcaacagcg cctttgccac cttccttagt ggctccttca acctgatagg
     6301 ctgctgtggc tccgccaaaa ataaaatctt ctggtaattt aagcatgtac atctctcctc
     6361 aaatctataa tcagttttcg ctctttctaa ctgattgcat tgtaaacgca tccaaaacat
     6421 ttgtcaacaa aaacaaacac aaataatcat aaaaaccaga aaacagcaat aaaatctatt
     6481 gtaacaaaca taaaaaccac cctaaatgtt tacttttgtt cgaattagat tgactgttgt
     6541 ccgtttatat gatagattat ccttaaatta gaggtgaaga caatgttgaa aaaagaaaga
     6601 ttgcttacta tcgtagaaat ggtcaataaa aaaggaatcc ttaccgttaa tgaaatcatc
     6661 aatcaattag atgtttctga tatgaccgtt cgcagagatt tagatgaatt agaaaaatcg
     6721 ggcaagctgc tgcgtgtcca tggaggggct caaagtcttt cctacacttt agatcaagaa
     6781 ctttctcaca ctgaaaaagc cactctgcag attcaagaaa aaaaagaaat tgctgccaca
     6841 gcagcctcct tgattcacga gggagaaacg attttcttag gaccaggtac aacaatcgaa
     6901 ctattggcga cgcatttact gaacaagaaa attcggatca ttacaaataa ttatccagtt
     6961 tttgatattc ttgctcaagc agatggacca gaaattctac tgattggcgg tgatttccgg
     7021 aaaaataccg gcgcatttgt cggaccgatc accaacgaca atttaagaag actaaagttt
     7081 acaaaagcat ttatcagttc aaatggcgtt cacaacgaag cgatctctac ttacagtacc
     7141 gaagaaggag aagcacagca gatcgctcta aataattccc gtaagaaata tctactcgtt
     7201 gataataaaa aattcaatcg agatgatttt tatgtgtttt ataatttgca tgatttcgat
     7261 ctgctcatta cggatcgcca gatctcgcgg gaggttcaag agcactatgc acaatatgtc
     7321 gatctgcaaa ttgctgattc aaccgaaaat accactatag gagcgtaact atgaaaggga
     7381 ttatatttga tttcaacgga acaatgtttt tggactctca tctgcatgaa gctgcttggc
     7441 tgcatatgat acatgatcat acaaaagatg gtttatcaga cgaggatatt ttaaaaaata
     7501 ttcatggtcg gacgaatacg gagattttga aacatttcat ttcagagcat ttgaccgcct
     7561 ctgagatcgc cgtattgtca gaagaaaaag aaagctatta tcgtatgctt tgcttgcaaa
     7621 atgaaaacga gcttcagttg acaaaagggc ttgaagcgac tcttgaccag ctgactgacc
     7681 aacagattcc attaacgatt gccacggcta ctgtcaaaga gaatgttgct ttttattttg
     7741 atattttcga tttagcccgc tggtttgact gggacaagat tgtctatgac gacggctctt
     7801 tccctgggaa accgcaaccg gatatttttc ttaaagcggc agacaaactg agcttagctc
     7861 cggctgattg tcttgttatc gaagatgctt actcaggatt acttgctgcc aaacgtgcgg
     7921 ggatcggaac aattattgca attgatccat tcggtaaaaa caaaacagta tttgaacagg
     7981 cacagctaaa taaagatgga accatcaaag attttactgg tttttggaca gagcacctgt
     8041 ctgccacatc cgttaattct taaacgattc aactaactac caaagaaaag caactgaaat
     8101 gcacacctcc ttttataaaa aactgccagt gaaacgacat gaaagataca ctgctatatc
     8161 cttagtgaaa tatagcagtg tatctttgac tttttcattc ttttaaccat atgacgcgtt
     8221 catctctatc tataattaac attaaaaata gtgttttcac attatttctc aaaccaatct
     8281 taaaaatcat caggagtact tttttgatat acttttatta atgctgggcg taatggttaa
     8341 aagatttcca ctagttcctc tccaccaagc tgagttgaaa aatgcttatc tgtaaacaac
     8401 atagacaaag aggaaataaa cgtttccgta tctttctctg tcccaaggat attaattaat
     8461 tttcctacta ggtaagtatc attaaactta agatctgcct tcacatcaaa agttttgata
     8521 aaagctttta tcatgcgtac aattttgtct ttatcgccat aattgatttc accactcata
     8581 tcaatatagt cactaatccc tgaagaaata atggcaactg cttcttcttt cgccttatca
     8641 gataattcat ttaatgactg ggattgattg atgacttctt gagttttatc tgttacgtaa
     8701 tctctcatta tcctatcggc atactccgca aagtaatctt gaaaaaattc ttctacaggg
     8761 taaccaactt cgttttccca accaggagaa atttcattat cagtacttat taaatctatt
     8821 aaaaaccaac ccgttctctg agtgttgatt ctataatatt tttaatttc
//
LOCUS       DACEXQ010000105         8828 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.105, whole genome shotgun sequence.
ACCESSION   DACEXQ010000105 DACEXQ010000000
VERSION     DACEXQ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8828
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.105"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            15..641
                     /locus_tag="IXH04_001681"
     CDS             15..641
                     /locus_tag="IXH04_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ1852284.1"
                     /translation="MPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQIPSATIR
                     RDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKHNNFRRNE
                     NLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYSQSAIDEI
                     VKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            1034..3679
                     /locus_tag="IXH04_001682"
     CDS             1034..3679
                     /locus_tag="IXH04_001682"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAQ1852285.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            3989..5302
                     /locus_tag="IXH04_001683"
     CDS             3989..5302
                     /locus_tag="IXH04_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAQ1852286.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5292..5945
                     /locus_tag="IXH04_001684"
     CDS             5292..5945
                     /locus_tag="IXH04_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1852287.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6000..6671
                     /gene="radC"
                     /locus_tag="IXH04_001685"
     CDS             6000..6671
                     /gene="radC"
                     /locus_tag="IXH04_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HAQ1852288.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGIYKLVHINYIRKFTVILPSGNTIMLSIIERHNAFFSS
                     SEDSL"
     gene            complement(6566..7918)
                     /locus_tag="IXH04_001686"
     CDS             complement(6566..7918)
                     /locus_tag="IXH04_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1852289.1"
                     /translation="MKRVALYMRVSTEQQAKHGDSLREQKETLYEYIEQHKDLKVVNE
                     YVDGGISGQKINRDEFQKLLQDVKENKIDLILFTKLDRWFRNLRHYLNTQEILEKHNV
                     SWNAVSQQYYDTTTAYGRTFIAQVMSFAELEAQIDSERIKAVMANKIAQGEVVSGKTP
                     LGYSIENKKLVINDDAPIVIDIFNYFLSSGSLRKTVYYLGSQYGIVRDYQSVKNMLTN
                     KKYIGELRNNKNYCPPIIDKKLFYAVQKALPKNLKTNAKRDYIFKGLLKCSDCQGSVA
                     GQTIKARYKKKDGTESIYERTCYRCVKRRNNKLRCTNKRAFYEKNLERYIFEATKQKF
                     EQIQINYSKKQPKIIKKKNSKKNIENKLDRLKKAYLNEVIDLEEYKKDREALMKELNE
                     IEVEPAKIDIKNVEFILSKEFDEIYKESSEEEKNALWRSIIDNIIVFPDGNITVNFLI
                     "
     gene            complement(7984..>8828)
                     /locus_tag="IXH04_001687"
     CDS             complement(7984..>8828)
                     /locus_tag="IXH04_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HAQ1852290.1"
                     /translation="KKMLIPLESEITGLLNKLTISNVDSTREKIIRTFDKINKLFKND
                     EAEFKKELLELKLQELELNYSYIVKVNDEKEQQKAIKEQMIEEEKVRREIEREKKRID
                     KEERQFNSEITKLMAYMQKASADVEKELYADKIKELEEKLRELETVKEDVLQRELNTR
                     AGYVYVISNIGSFGEDVYKIGMTRRLEPMDRIKELSSASVPFEFDVHAMIFSEDAPTL
                     ENQLHSHFRKNEVNKVNQRKEFFKVSLDEIEKVVLENYNGTVSFTKLAQAEQYRRSLE
                     LTDV"
BASE COUNT         2798 a         1487 c         1721 g         2822 t
ORIGIN      
        1 aaaaaacaga tacactgcca aaagcaactg cgaaaagatt acctttatat ttacgctact
       61 tgaagatgct ggatgactca ggtataagta gaatcaaatc aaatgagttc agcgaaatca
      121 cccaaattcc ttctgcaacg atccgcagag acttctcgca attaggcgag ctaggcagaa
      181 gcggatatgg ctatgatgtc ccttttctta tcgatgtatt caataatatc cttaatacta
      241 aagaagaaaa acgaatcgca ttagtaggat atggaaatct tggcaaagca ttgaaacaca
      301 acaattttag aagaaatgaa aatctaaata tcgtgtgcgt ctttgataat gatccagcac
      361 tgatcaatcg agtaattgat ggagaaatga tctatccgat tgatcgtttc gcggagatag
      421 ccaaagcaaa aaatgtgaca gtagcaatct ctacggtacc aagtaaatat tcacaatctg
      481 ctattgatga aattgtcaaa ggaaatgtaa ccgctatact taattttgcg cctgacagag
      541 taactgtccc agcttatgtc aatgttcaat acattgattt gacaaccgag ttgcagacgc
      601 ttatttactt tgatgagaat tactcagaag ttttttctta ggaaaatttg agtaggctta
      661 ttgatcttgc gagaactcgt cgtaattctt tcaaaaattt tttagtacta aatgaagtgg
      721 ttttttcacg accacttgtt gactaaaaaa aaaaaccatt gtagaatgaa gcataacgct
      781 gaacaagagg agtaactggc acattcattc ttagagagag aactatctgc tgaaaggttc
      841 tcaatgacaa ccagtgaagg tagcttggga gtttctgtct tgaatggagt agagatagac
      901 gagtcgtgat cgttaactca cttgagaggc aataatgatt gattattgca atttaggtgg
      961 taccgcgtaa atcatacgtc ctagaatacg aaagtatttt aggacgtttt ttagtttaaa
     1021 ggaggaaaac agcatgacgg aagaaaataa cctatcaaca aaatataatc cgcaagaagt
     1081 tgaagctgga cgttatcagc attggttaga acaagatttg tttaaaccga gcggagataa
     1141 aaaagcaaaa ccttattcaa tcgtaatccc gcccccaaat gttactggaa aacttcattt
     1201 agggcatgct tgggatacga ccttgcaaga tatgctgatt cgtcaaaaga gaatgcaagg
     1261 atatgatact ttatggctgc ctggtatgga ccatgcagga atcgcgacac aagcaaaagt
     1321 agaagcgaag cttagagagc aagggatttc tcgttatgat ctaggtcgtg aaaagttcat
     1381 cgatcaagtg tgggaatgga aagaagagta tgcgagccac atccgcgaac agtgggctaa
     1441 aatgggctta tctttagatt acagtaggga acgttttaca ttagatgatg gattatctga
     1501 agcagtacgt aaagtttttg ttaccttata cgaaaaaggt ttgatttatc gtggtgaata
     1561 tatcatcaac tgggatcctc aagcacgaac agctctatct gatatcgaag ttattcataa
     1621 agatatcgaa ggcgcctttt atcatatgag ctatgaacta gcagatggat caggcgtagt
     1681 agaaattgcg acaacccgtc cagaaacgat gttgggagat acggcgattg ctgttcatcc
     1741 agaagacgag cgttatcaag cgctcatcgg caaaaaagtg attctacctt tagtcaataa
     1801 acaaatccca atcattgcag atgaatatgt agatatggaa ttcgggacag gtgtagtaaa
     1861 aatcactcca gcacatgatc caaacgactt tgaggtaggt aatcgtcatg atcttccacg
     1921 cgtaaatgtt atggatgatg atggtacgat gaatgaacta gcaggaaaat atgctggaat
     1981 ggaacgtttt gctgctcgta aagcaatcgt gcaagatctg aaagaaatcg gtcgattgat
     2041 taaaatcgat acgatgatcc acagtgtggg tcattccgag cggacaggtg tcgttgttga
     2101 acctcggctg tctactcaat ggtttgtaaa aatggcgccg ttagcgaaaa aagccattga
     2161 taaccaagaa acagatcaag cagtggaatt cttccctcca agattcaatc aaacgttttt
     2221 acgttggatg gaaaatgtcc atgattgggt aatctcacga caattatggt ggggacatca
     2281 aattcctgca tggtatcaca aagaaacagg tgaaatgtat gtagggatgg aagcaccagc
     2341 ggatagtgaa aactgggtac aagatcctga tgttttagac acttggttta gttcagcttt
     2401 gtggcctttc tcaacaatgg gctggccaga tacggaaagc gaagattata agcgatactt
     2461 ccctactagc actttagtaa ccggttatga catcattttc ttctgggtaa gcagaatgat
     2521 tttccaaagc ttggaattta ctggcgaacg tccatttgag aatgtcttga ttcatggatt
     2581 gattcgtgac gaacaagggc ggaagatgag taaatcatta ggtaatggaa tcgatccgat
     2641 ggaagtaatc gaaaagtatg gagcagatgc tctccgctgg ttcttatcga atggttctgc
     2701 accaggacaa gatgtccgct tcagctatga aaaaatggat gcttcttgga actttatcaa
     2761 caaaatctgg aatgcttcac gtttcgttat catgaatatc gaaggaatga ccgtagaaga
     2821 gatcgattta tctggagaaa aatcagtggc tgaccggtgg atccttactc gtttgaatga
     2881 aacgatcgaa cgagtaactg agctatttga tcgttttgaa tttggtgaag caggtcgaca
     2941 actgtataac tttatctggg atgatttttg tgactggtac atcgaaatga gtaaagaagt
     3001 actttatgga gatgatgctg tttcaaaaca aaccacccgt agcatcttag tccatacact
     3061 tgatcagatc ctacgtttat tgcatccaat catgccattc gttacagaag aaatatggga
     3121 acatattcct catcagggaa attctctagt agtggcagaa tatcctgttg tacgtccaga
     3181 gtttgatgat gaaacagctt ctaaagggat ggaagtattg aaagaattga ttcgagccgt
     3241 tcgaaatatt cgttcagaag tgaatacacc attgtctaaa ccaattacgt tgctaattaa
     3301 aacaaacgat gctaaaatcg atcaattttt agttgaaaat acaagctata tcgaacgctt
     3361 ctgtaatccc gaagagttga cgatatcaag tgagatcact gcgccagatc tagcgatgtc
     3421 agctgttctg acaggtgcag aaatcttctt gccgttagca ggattgatca atattgaaga
     3481 agaaatcaaa cgattagaaa aagaactgga aaagtggaca agcgaagtaa aacgtgtcca
     3541 aggaaaactt tctaatgaac gttttgtttc taacgcgccg gaagaagtag tagaggcaga
     3601 gcgtgcaaaa gaaaaagatt atttagagaa gcaaacggct gtcaaagaac gaatcgaaag
     3661 tttgcgtact attcaataag aatagaaaaa gagagtgtga aatcagctag ctattactaa
     3721 aagctagctg attgaacatc gttacaggaa agagtttgag cctcactctt agcttcgatt
     3781 cataaggaga tgtgacataa attttgtcgc atctcttttt tagtctactc ttattatgta
     3841 ggagaaggag ggaggtggat catgtatcaa gagtcaataa agtagttgat aaaatcgttc
     3901 ctctctctgt ttcttttctc tttgtcatac ttttattttt cttatgtgtt ctatttgtta
     3961 taataaaaga gaaatggggg aattgttgat gacgattgaa gaagcaatag aatggatcca
     4021 tagtcgatta ccatttggtt cacgtcctgg ccttgaccga gtcgaagctt tattagaagt
     4081 ggtagggcat ccagagagaa aagtaaaaac gatccatatc gctggaacaa acggaaaagg
     4141 ttctactgta gcttatttga gaagcctatt agaagaggct gggctgacag taggtacatt
     4201 tacttcacct tatatcgaaa cgttcaatga acggatcgca atcaatggca aaagcattcc
     4261 agacaatgat ttgatccagc ttgtaaaaaa atatcagcca ctgatcaaac aacttgatca
     4321 atttgaagca gtagctggta ttactgaatt cgagacatta acagcaatgg catttgatta
     4381 ttttttgatg aagaaagtgg acgtagcgat tatcgaagtt ggcttaggcg gcttattgga
     4441 ttctaccaat gtagctgtac caatgctgac gggaataaca acgattggac ttgatcatac
     4501 cgaaatactt ggagatacaa ttgaagaaat cgctgcacaa aaagctggaa tcatcaaaca
     4561 aggcgttcca gtagtaacag gaaatattgc cccagaagct tttactgtca tccaacaaac
     4621 agctaaagca aaaaatgcac cgctttatgt atacggggaa aattataaaa taaactatct
     4681 gcatcctgat gagatgtggg gagaagtatt tgaatttttt aatgaaatag gaaagatccc
     4741 tcaattaacc actccgcttt tagggcaaca tcaagctgaa aatgcaggag tagccatcga
     4801 attgtatttt gtttactgtc aattggaaaa gctgcctttc cgagaaaaag atgttttgaa
     4861 gggattgaag aaatctcgat ggccaggaag aatggagcgt ttgagtaacg agccattagt
     4921 tgttttagat ggtgcccata atacgcatgc aatgaaccgg cttgttgaga atttgaaaaa
     4981 agaattcaaa ggatatcaaa tcaacatttt gttttccgcc ttacgaacta aaaatgttag
     5041 agaaatgcta gaacgattgc agcaagtacc aaatgtccat atttatttga cgacctttga
     5101 acatccaaaa gccatcgaat taagtgaagt atcagattta gcaagtgaga gagtatcaat
     5161 cgtttcattg tggcaattcg gattagcaga tatattggaa aagatgacaa atgaagacgt
     5221 attagttgtg accggttcac tttatttcat ttcagaagtt cgccatttat taatacaatt
     5281 aggaggagat catgaagtat aatgcagtga tttttgacat ggatggtttg ctgttcgaca
     5341 cagagttagt gtattacgaa gccacacaag tagtagcaga caagatgggt tttccttatg
     5401 ataaagacct gtatttaaaa ttcttaggtg tttcagatga agaagtatgg gcgaattatc
     5461 atatgatttt ctcggaattt ggcaaaacaa gtgtacaaag attcatagat gattcatacc
     5521 aggaaacgct tgaacgtttt tcgcaaggag cggtacaaat gaagcccggt gtgttggaac
     5581 tcctctcttt tttagaagaa catcagattc ccaaagttgt cgcttccagt aaccaacgag
     5641 cagtcattga acttttattg aagaagaaca atctgtttga acgatttgag acgattgttt
     5701 ctgcagaaaa tgttcaacga gcaaaaccag acccagaaat tttcttgtta gctaatcaga
     5761 agctaggtac agcgaagaaa gaaacattga ttttagaaga ttcacaaaat ggtattttag
     5821 cagcttctgc ggctgagatc ccggtagtca tggtaccaga tctgcttcca ccatcccaag
     5881 aattgatcaa aaaaacagct ggggttgttt cttctttaca tgaaatccct gcattgttaa
     5941 aataaaaatc catctctttt cgtatcttat tttagataag atagaaagga gatgtttttt
     6001 tgggaaaacg attgatcaaa gaagtaccta ctagctcttt accaagggaa cgtatggaaa
     6061 tttatggcgc ggaagcttta tcggatcagg agcttttggc aatcttactg cgtaccggtc
     6121 aacaccctta tagtgtgatg tcgattgctg gaaatctgtt aaaaacgttt ggaggactgg
     6181 cctctttacg acaggctact ttgcatgagt tagaagagat tcgagggata ggacgcgtga
     6241 aagcaatcga aatcaaagcc ttgatcgaat taggtcgacg aattcagacg gatcatgaaa
     6301 gagcggctcc accggttcgt accagctacg aattggccca gcaattgatt ctggaaatga
     6361 aagatcacaa acaagaacat ttggtttgta tttatttgga cacgaagaat caagtgatct
     6421 taaaaaaaac agtgttcatc ggttcgctga atcagtcagt cgctcatcct agagaaattt
     6481 tccattatgc tgtacgttat tgtgcagcaa ggatcgtttt agcgcataat catccttccg
     6541 gaatatataa gttagtacac ataaattata taaggaaatt tactgttata ttgccatctg
     6601 gaaatactat tatgttatcg ataattgaac gccataatgc atttttttct tcttccgaag
     6661 attctttata aatttcatcg aattcttttg atagaataaa ttcgacattt tttatatcta
     6721 ttttagctgg ctctacttca atttcattta attctttcat taaagcttct cgatcttttt
     6781 tgtattcctc taagtctata acttcattca agtaggcttt cttcaatcta tctaatttat
     6841 tctcaatatt tttcttacta tttttctttt taataatctt tggttgtttt tttgaatagt
     6901 ttatttgtat ttgttcaaat ttttgtttag tagcttcaaa aatataacgt tctaaatttt
     6961 tttcgtaaaa agcgcgctta tttgtgcaac gtaatttatt atttcttcgt tttacacatc
     7021 tataacaagt cctttcgtat atagattctg taccgtcttt tttcttatat cttgctttaa
     7081 tagtttgacc agctactgat ccctgacagt cagaacattt taataaacct ttaaaaatgt
     7141 aatcacgttt tgcatttgtt tttaaatttt taggtaaggc tttttgaaca gcataaaaaa
     7201 gttttttatc aataatagga ggacagtaat ttttattatt tcttaattca ccaatgtatt
     7261 ttttgttcgt caacatattt ttaacacttt gataatctct aacgattcca tactgagagc
     7321 ctaaataata aacagttttt cttaaactac cactagaaag aaagtagttg aatatatcta
     7381 tcacgatagg agcatcatca tttattacta atttcttatt ttctatagag tagcctaacg
     7441 gagttttccc gcttactact tcaccttgtg caattttatt tgccataaca gctttgatgc
     7501 gttctgaatc aatttgggct tccaattcag caaaactcat tacttgtgca atgaaagttc
     7561 tgccatacgc tgtagttgta tcataatatt gttgagaaac agcattccaa gaaacattgt
     7621 gtttttctaa aatttcttga gtgtttaaat aatgtcgtag atttctaaac catctatcta
     7681 attttgtaaa taaaattaga tctattttat tttcttttac atcttgtaat agtttttgaa
     7741 attcatcacg atttattttt tgaccagaaa taccaccgtc tacatattca ttaactactt
     7801 tcaaatcctt gtgttgttcg atatattcat agagggtttc tttttgttct ctaagactat
     7861 ctccatgttt ggcttgttgt tcagttgata ctctcatata taatgctact cgtttcatgt
     7921 tatctccttt cgaatgtatg ttcttatcgt tgtaaaagaa aagcccgaag gctaatcttt
     7981 ttactacaca tctgttaatt ctaatgatct tctgtattgt tcagcttgag ctagtttagt
     8041 aaaagataca gttccgttat aattttctaa aacaactttt tcaatttcat caagagatac
     8101 tttaaagaat tcttttcgtt gattaacttt attgacttcg ttttttctaa aatgcgaatg
     8161 tagttgattt tctaatgttg gtgcatcttc tgaaaatatc attgcatgaa catcaaattc
     8221 aaaaggtaca gaagcgctac taagttcctt aattctatcc ataggttcta accgtctagt
     8281 cattcctatt ttatagacat cttcaccgaa agatccgata ttactgatga cataaacata
     8341 gccagctcta gtattaagtt ctctttgcaa tacatcttct ttgacagttt ctagttctct
     8401 taatttctct tccagttctt taattttatc agcatagagt tctttttcaa catcagcact
     8461 agctttttgc atgtatgcca ttaacttagt tatctctgag ttaaactgtc gttcttcttt
     8521 atcaatgcgt ttcttttcac gttctatttc acgtcttacc ttttcttctt caatcatttg
     8581 ttcttttatt gctttttgct gttctttttc atcgttaact ttaactatgt atgaatagtt
     8641 gagttctaat tcttgaagtt ttaattcaag taattctttt ttgaattctg cttcatcgtt
     8701 tttaaaaagc ttgtttattt tatcaaaagt tcttataatt ttttctctag ttgaatctac
     8761 gttagaaata gtaagtttat tgagaagtcc agttatttct gattctaatg gtataagcat
     8821 tttctttt
//
LOCUS       DACEXQ010000106         8818 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.106, whole genome shotgun sequence.
ACCESSION   DACEXQ010000106 DACEXQ010000000
VERSION     DACEXQ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.106"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(310..807)
                     /locus_tag="IXH04_001688"
     CDS             complement(310..807)
                     /locus_tag="IXH04_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1852291.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(847..1656)
                     /locus_tag="IXH04_001689"
     CDS             complement(847..1656)
                     /locus_tag="IXH04_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1852292.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIDMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            1990..2847
                     /locus_tag="IXH04_001690"
     CDS             1990..2847
                     /locus_tag="IXH04_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ1852293.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            2840..3742
                     /locus_tag="IXH04_001691"
     CDS             2840..3742
                     /locus_tag="IXH04_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ1852294.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            3775..4125
                     /locus_tag="IXH04_001692"
     CDS             3775..4125
                     /locus_tag="IXH04_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ1852295.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            4159..5097
                     /locus_tag="IXH04_001693"
     CDS             4159..5097
                     /locus_tag="IXH04_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ1852296.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            5175..6068
                     /locus_tag="IXH04_001694"
     CDS             5175..6068
                     /locus_tag="IXH04_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1852297.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            6106..6669
                     /gene="amaP"
                     /locus_tag="IXH04_001695"
     CDS             6106..6669
                     /gene="amaP"
                     /locus_tag="IXH04_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ1852298.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            6683..6871
                     /locus_tag="IXH04_001696"
     CDS             6683..6871
                     /locus_tag="IXH04_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ1852299.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            6884..7786
                     /locus_tag="IXH04_001697"
     CDS             6884..7786
                     /locus_tag="IXH04_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ1852300.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            7818..8060
                     /locus_tag="IXH04_001698"
     CDS             7818..8060
                     /locus_tag="IXH04_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ1852301.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(8316..8687)
                     /locus_tag="IXH04_001699"
     CDS             complement(8316..8687)
                     /locus_tag="IXH04_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852302.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         3073 a         1522 c         1706 g         2517 t
ORIGIN      
        1 ttggctgttc ttattactgc agatgcaagc ttttgaatac cgtttaagga agaggttgtg
       61 aaatcagcgt tttgacctga gtattagctt aacaaacgga aaaatagttc ctcatatttt
      121 gccgtttgtt aagctaatac cgagggtcag ctgattgaac accatttaag aaagaggtcg
      181 tgagcctgcc gtttcactct gtgtcataaa aaagccaatc tactttgata gaaaaagcac
      241 gtaggactaa aacagttctg cgtgcttttt ttattgtgtg ttaaatatcg taaatgaaga
      301 tagatatttt tatgattcga cgatagagac gttgaaatct ttcaacagct ctaaagcgga
      361 ttctccgtgt tttctactgg agactaacat tggatcaaca aaaaagtctg cttcaggaaa
      421 agcactctgc aggcctatca gattagagag tacacacaag tttgtttcaa tcccgactac
      481 ttcgatctct ttttgttctt ctttgctttc aaataaagtt tcctggattt cagctaaagt
      541 ttcaggtgga atcgtaaagt aatacttttt aatcactcgt tcattttctt gtggagaaag
      601 aagagggatc aattgcaaat cttctcgttc ttcttctttg tccttcagtt caactggtat
      661 atcccgtgtg aagagaatat attcattatt ttggcgtgct ttagctaatc tttgagcaat
      721 acgttctgcc aattgatctg actctggaat ataaaattcg ctgtttggat caagaatatg
      781 attttgcata tcaataataa ctaacatttt ctttcctcct acattcatat cgtcatttta
      841 ttctttttaa tagaagtttt ttaaaatata agcaacacca gcttcttcat tcgtcagtgt
      901 tacttggttt gcaatttctt ttacttgtcg ctgtgcattc cccatcgcaa tactcaaccc
      961 gctgtaattc aacatatcaa tgtcgttttc ttgatcgcca aaagccatga tgtcactcga
     1021 ttcgattcca aggtaatccg ccagaaaaac aagtgaatga gctttagtga tacctgtagg
     1081 cgtgatttct aagaaatgcg gatcagagaa tactgattgg ataccatctg cttgcagatc
     1141 tctttgtagc ttctgtagtt tttcttcact cttatctgtt aatgctaatt taagatagct
     1201 aagagaagga tcttccaaca tatttaaaat ttgatcaaaa gaatggttct gtaaattgaa
     1261 atgttcataa taagggtcat ctttgactag atttgattca tcgaatgtca gctgatcatc
     1321 ttgggtttct cggctagcaa tgatggatat tttcgcttct tgagcataga agaaggcttt
     1381 tctcagtgta tcgctatcta tttttgaacg tctgagaact ttcgccattt ttccatcctt
     1441 catttcagca atcagtgaac cattggaaga aatcaaatag ccaccgattc ctaattcgga
     1501 aaaaatatct ttagcagaaa tgctgttcct tccggtagcg acggttacga ttccgccatt
     1561 ttcatcaaat ttccgaagtg cttggatatt ttcttctggt atctttccat ttcgatcaag
     1621 caaggtaccg tctaaatcaa tcgctgctag tttcatttta tcttcctact ttctttttac
     1681 atatctattt tcctgttacc ttaagattaa atgagaattt aaaaaaatga aactaacttg
     1741 ctttgtcatt atgaattttt tcaaaaaaca ttcttaagaa aaaggtggct atatatagta
     1801 gtttttggtc aatacctttg aataaaaagc catgaaaaac gaatagttag cgcttaccga
     1861 tgcgttttgt gataaaaaaa ttttaaaata ttcaatgaaa acaaagcata tattttgctt
     1921 taaaaccaaa tgaggaatat tctagatgta gaacaggtta gaaaaagatt agaaaaagga
     1981 gtgattttga tggagaagag atacggcgta gttcctgtgg taactgcttc agacgaaaat
     2041 tatgctcctt acttgagcgt gatgatcgca actgcattag aaaattgcaa taaaacaaga
     2101 cgcataaaat tttatgtgat tgacgatggt ctatcagaat atagtaaaga aggattggaa
     2161 gaaacggtaa ataaatattc aagtaatgca agtatccaat tcctgacagt ggaaaaagat
     2221 atctacgaag acttcctagt cagtgaccat atcacaacta ccgcttactt aagaatttct
     2281 ttaccaaact tattagctaa agaggattat aagaaagtcc tttacttaga ttcagatgtg
     2341 ttagtactag acgatatcgt aaaactatat gatgagccgt taaatggaaa aacaatcggc
     2401 gctattatcg atccaggcca agtgaaagct ttggaaagat taggaatcga ttccgatgac
     2461 ttgtacttca attcaggagt gatggtgatt gacattgatc aatggaataa aaaagaaatt
     2521 acagaaaaaa ctatccatta cctcagtgaa aatggcgatc gaatcatcta tcacgaccaa
     2581 gatgctttga atgctgtact ttatgaagat tgggaacaac ttcatccaaa atggaacatg
     2641 cagacttcgc tgatttttga acgtcatcca gcaccaaatg aaaaatatga acgtcagtat
     2701 aaagaaggaa atgaaaaacc ttcgatcgtt cactttacag gacatgataa accttggaat
     2761 acgttgaaag atcatccgta taccaattta tacttaaaaa aattagcaca cagtactcta
     2821 acgaaagtag gcgagatcaa tgaataaaaa agaaatagca gtagtcgcaa gttgtaacac
     2881 aaaattcgta ccacatttag ctgcattatt tgtctcagta ttagataatt gcaacccgtc
     2941 taaatttgtt cggttctatg tgattgacga tgatatcgat tttgaaagta aacagctttt
     3001 acgcttctca gtgaaaaacg cacgaatgaa ctcagatgtg gaattcttga aaatcaataa
     3061 agaattcttt acaaatgtgg taatcagcga tcgtattcca gaaacagctt attatcgtat
     3121 tgcgattccg gaattgttcc gcggcacaga agttgaacgt atcttatata tggattgcga
     3181 catgatcgct ttacaagata tctctaaatt atggcgtctt gacttcggtg attcaatcgt
     3241 agcagctgtt gaagacgctg ggttccatca acgactggaa aaaatgaaaa tttcagctaa
     3301 atcaatgcgt tatttcaact caggtttgat gctgatcaat gtgaaaaaat ggttagatga
     3361 aaatattacg caaaaagtat tagatttcat cgaacataat ccagaaaaat tacgattcca
     3421 tgaccaagat gcattaaatg ctatcttaca tgaccgttgg ttaccacttc atccgcgttg
     3481 gaatgcacaa ggctatatca tggcaaaagc aaaaaaacat ccaacagcag caggtgaacg
     3541 tgaatacgaa gaaactcgca acaatcctta cattatccat ttttcaggac atgtgaaacc
     3601 gtggagcaag gattttgaag ggccaacgaa gaaatattac gaaaaatatg cagggatgac
     3661 ggctttccgt tgtgtagcta aattccctaa atatccaaaa tatgcaaaag ttcaaccaaa
     3721 aacagatgta gttgttaaat aatagaagac aacaactgaa gagggggagt aggaatggaa
     3781 attacaatgt ctaaaaatgc agtagagacg ttaattgaaa aggttgggga aaatacaaaa
     3841 atcgcactcg cgcttatcaa tgattccgat ccatttctaa gggacaaagg tgcttttgca
     3901 aaaggcagct tttttcaaat cattcctttt gtttcggaat ttggagaata tgcgactaaa
     3961 atcgagcatc cattattgga catctataca tctaaattag agcaaaatta tttcggtaaa
     4021 cgattgaaca tggacttcaa caaacagctt gacagctttt cattagaaaa tgaaatcgca
     4081 gtactcgatt ataatatcaa attaaagaac tgcttcttca gctaatagtt tcgagaaagt
     4141 ccaaaaagga ggtatacatt gagatactac tttgtggatg aagaaaagaa tgtaatagaa
     4201 acagatgaaa aaaaatataa ttggctcgta cttgatagtg caaagcccga agagattgag
     4261 caagcaatcg aagaattcga gcttcctgat gatattttcg taggaaccga atatccggaa
     4321 gaagtttctc ggatggagcg cctttatgag acgaaactag agcatccatt ttctttggta
     4381 gtgattaatc tagatcagtc agataatcgt atcgaaaaaa aattaacgcc aatatctttt
     4441 gtactttccg ataatctctt gattacttgt atcgatagaa aaaccgaatt tgtggatcgg
     4501 ctgctgacaa agcatggaaa tcatttaaac tcgtttgaaa agatcatcac gtatacatta
     4561 ttagatattt acacccatta cgtgaaagaa ctaagagaga tgaaaaagcg aattgacgct
     4621 ttggataaag cagctcgtaa aacaacggaa aatgaagaac tgtataaaca ggcagatctt
     4681 gaaagagaca tcgtatatat cgatcatacc ttaagagatc aacgggaaac aatggatcaa
     4741 ttatggaatt ctgaaacttt catcaaacgt ttagacgatg aacagctact ctatgatgtc
     4801 catttgagac agcgacaaac agaaaaaatg atcgagatct atcgagattt attagagagt
     4861 attggtgatc tattcaacgg gatgatggac aataatctaa atcatatcat gaaatatcta
     4921 gattcagctg cattaatcat ttccgttccg gcgttgttcg cagggatttg gggaatgaat
     4981 acaggaggat taccgggtaa aagttctgct ctaggcttta tactcgtagt ctttggctct
     5041 ttgatagcag cagtagtttt aggttaccat ttataccgga aagattatac aaattaaaga
     5101 atctatttaa aacaggtatc aaacaaagaa atttgttttg gcaatacaat ttaaaaacta
     5161 ggaggaatac agcaatgaca gaaccaaaat taatcgaccc gcgaaacttg taccatacgg
     5221 agaaatttcc taaacaagat caggatacac cagctttaca gcagaatatg attcctgttc
     5281 ctgattgtgg agaagaatcc tatgaaggca ataatcaact agaaaaccgc cgtgtactaa
     5341 tcactggtgg agattcaggg atcggtcgtg cagcagcgat tgcatttgct agagaagggg
     5401 cagatatcgc tcttcaattc ttcccaggag aagaaaaaga tgcagaggaa gtagcaaaat
     5461 acgtgaaagt tgcaggacgt aagattgtct tactaccttt tgatcttcgt gacgaacaag
     5521 cacctaaaga aatcatagat aaagccgtga gtgaacttgg cgggctagat acgatggtac
     5581 taaacgcagg acagcaaatt tcttgtcagt ctattgctga gttaccactt gagcaagtca
     5641 aagacacatt tatgataaac atcatcgcta tgtttgcttt agtgaaagaa gctgttccgc
     5701 atatcccagc aggaggagca atcgtaacaa caacttctgt tcaagcattc aatccaagcg
     5761 aacatttatt agattacgca gcaacaaaag cttcaatcgc taactttacg atcggtttag
     5821 caaaacagct tgcacctaaa ggaatccgtg taaatggtgt agcacctggt ccaatctgga
     5881 caccattaca actagacgaa ggacaacctt ctgattcgat ccctgaattt ggtcagcaaa
     5941 gtttactaga acgtgcaggt cagccaggag aattagcacc agtttatgtt ttcttagcat
     6001 cgaataaagc aagttatgtt acagcccaaa tttacggagt aactggtgga gaagcgatca
     6061 acttataaga agttgatcgt ttcaccccta tgaggaggtg cgaaaatgaa taaaggtgta
     6121 aaaacgatcg gagtgatcgg aacattactt ttgctatctg tgcttttgtt tgcgatacta
     6181 ttaagtgcaa cctatccatt gccttttcag ctagagaagt ttcgattctt ttccatcact
     6241 aattcatacg tgcaacaata tgtattttgg cttgccgttg catttgctat tttgacaatt
     6301 atcacgttac ttgttttcct gttttatcca aaaacaatcg gaaactttct actgaaacat
     6361 ggtgatgggg agttgacgtt ggataaaaaa gcaattgaag gaatggttcg ttctcacttg
     6421 catgaagatg aattcatcca ttcaccaaaa gtaaatgtca aagcaacgaa aaacaagatc
     6481 gccgtcaatg taaaagggga gttgaaacga acttcagcac tcattggtaa aacaggaaca
     6541 ttgatgaaag aaatcgaaaa tgaagtacaa cacatattgg gaacaaaaga gccagtgaaa
     6601 gtatcggtga aatacacagg ctttgaagaa ccagctaaac gaagcaatga ccgcaaacga
     6661 gtagaatagg gggggaccaa aaatgaaaga actattttcg acatacaagc tccctatcat
     6721 ctttggggta cttggattga tattagccgt tttatggatc accgtgggct ttttcaagac
     6781 cttattgatc ttgatcatgg cagcgatcgg cgtcgctatc ggtttttatc tgaaacaaac
     6841 gaaaatctta gatcactatt ttaaatctta ggaggaaaca tccatgaata acaataacaa
     6901 aaataataac aataaaatga acaaccaaaa accagtgtct gcacatccag acccaacgac
     6961 accaaaccaa tcaaatggtg tgaacgatga aaagaacaaa acagcaccag tatctgcaca
     7021 tccagatcca gtagtcccta acgttcaaca aggagtagaa gagaagaaac atgatgcaga
     7081 aaagcatgca cctcatcatc caggaactcc agaatctaaa gttccagaac aacatgatgc
     7141 aatcgatcct tctaaacctg tggaagagca aaaacatcct tctgaccatg cgaaagcaca
     7201 agaaaaactt agttctgacc acacaaacgg acaaggaaaa cttggttctg atcatacaaa
     7261 taaccaagga aagcatgatt ctgaacagga agattcaatg cgcggtgagc tgaattatga
     7321 agataaagtt gtgcaaaaaa tcatcggaat cgcaattgaa caagtagacg gtttattatc
     7381 tgcaaacggt ggtttcttct ctaatgtggc aggaaaactt gtgaatacag ataatgtaac
     7441 ggctgggatc gaaacagaag tcggtaaaaa acaagtagca gttgatatgg atgtcattgt
     7501 ggaatatggc aaagatatcg aaaaaatctt tgaagaaatg caagaagtaa tcggcaaaga
     7561 agtaaaaaat atgacacatt tagaagtgat cgaagtcaac gctaacgtag tggacatcaa
     7621 aacaaaagaa gaatttgaaa aagatcaaga aacggtgcaa gacaaagtaa ctgacgcagc
     7681 gaaaaaaacc ggtgaatttg cttcgaatca aacagataaa gtaaaatcag cagctggaag
     7741 cggcgcacaa aaagtaaaag aaaatacaga accacgggtt cagtaataag gaagtaaaac
     7801 attaggagga acgaaacatg ggatttattt ggtcacttat cgtcggagga attatcggag
     7861 caattgcagg agcaatcacg aataaaggtt cttcgatggg gattattgca aatgtcgtag
     7921 caggtttagt tggatcagca cttggtcaag cattattagg ttcttgggga ccaagtattg
     7981 caggaatggc aatcgtgcca tcattgattg gtgccatcat tttagtacta atcgtatcat
     8041 tctttgctcg aaaactgtaa aaagactttt aaaaagaggt cgtgaaaaag ctgttcttca
     8101 tcaagtaata agaacagcca aacaaaaata gcttctcata tttttcattt ggttgggcat
     8161 attatcgaag atgcaagctt ttgaacactg tttatacgga ataagaggct gtgacaagac
     8221 ttttgtcaca gcctcttatt tatgcgggca aattcccaat tagattttta aaagtttttc
     8281 cttaaatgtg tctttattga tacagagaag cttctttaat aaaacaatgc ctccctaaat
     8341 tttcttgaga taggaaaggt gaacatacaa aataatacga ccactttgaa aacccagatg
     8401 atccacaaat ggatcgtatg atactgatga agtaaaaagc aatggatcag aaactcaaag
     8461 agattgaaaa agacaaggaa gaaataatat ctctttttta ctcttgtctg ttttagccaa
     8521 taatagctcg taaagtaatc tagacaaaca aatagaagta aacaaagttt cgtaagagca
     8581 gcaatcgtat catgttgctc tgctagacga ttgacgagca ggaaaaatgt tggtaatgga
     8641 atatagatca cgagtaatga aagaaaaaag taaacaataa atggcatact attcctccta
     8701 attgtgtgag ttcgccctca ttttaaaata acgatatttg tttgtgaagc tttttcgtgt
     8761 cgtaacaaat gttatctaat gtatttttaa ataaataatc attattatca aaataatg
//
LOCUS       DACEXQ010000107         8674 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.107, whole genome shotgun sequence.
ACCESSION   DACEXQ010000107 DACEXQ010000000
VERSION     DACEXQ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8674
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.107"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(26..319)
                     /locus_tag="IXH04_001700"
     CDS             complement(26..319)
                     /locus_tag="IXH04_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAQ1852303.1"
                     /translation="MELTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(347..1012)
                     /locus_tag="IXH04_001701"
     CDS             complement(347..1012)
                     /locus_tag="IXH04_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAQ1852304.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(1152..1784)
                     /locus_tag="IXH04_001702"
     CDS             complement(1152..1784)
                     /locus_tag="IXH04_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1852305.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     ALRPDVILMDLVMEEMDGIESTKAILKDCPKAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(1791..2858)
                     /locus_tag="IXH04_001703"
     CDS             complement(1791..2858)
                     /locus_tag="IXH04_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ1852306.1"
                     /translation="MMGRISKAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLMAISLGVGLIVFLLLSLIQKAKYGKIEEKLRALSSGNYESKLLHQP
                     IPSASDDLYIKDIDMEIAKIKEKMIEVSSELQIMTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLTSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(2855..3586)
                     /locus_tag="IXH04_001704"
     CDS             complement(2855..3586)
                     /locus_tag="IXH04_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAQ1852307.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDVEVIRI"
     gene            complement(3754..4233)
                     /gene="greA"
                     /locus_tag="IXH04_001705"
     CDS             complement(3754..4233)
                     /gene="greA"
                     /locus_tag="IXH04_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAQ1852308.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGD
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(4369..5544)
                     /gene="mltG"
                     /locus_tag="IXH04_001706"
     CDS             complement(4369..5544)
                     /gene="mltG"
                     /locus_tag="IXH04_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAQ1852309.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPNGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELYQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEKAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVQKYVNNS"
     gene            5709..6869
                     /locus_tag="IXH04_001707"
     CDS             5709..6869
                     /locus_tag="IXH04_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ1852310.1"
                     /translation="MAKGKHQKKQQISLSFLAVLLLAVSFFSTGIITGWQLTKQPQPQ
                     IYKTEPAQLASNLTSNEQMLASKIDQLLQKSDYIGSIYVRKNNRVILEKGYGYANLRI
                     KESNSPSLYYQIGSIQKAMTALLILKQIQSGKITFDTKLSEFYPQIPGSQQISIKNML
                     YMRSGLHRTASPKKPMSDSEIIQFALHHLKFTGYDTYHYEPLNYTLLTGVLIKLMNQP
                     YETLLKKEIIRPLHLEHTDFYENVKNSPQHAVSYQMSATDDYSKALVEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGDIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVAAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVNLKS"
     gene            complement(6909..7856)
                     /locus_tag="IXH04_001708"
     CDS             complement(6909..7856)
                     /locus_tag="IXH04_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAQ1852311.1"
                     /translation="MDTILITGGAGFIGSTLANYLGKENKIVVVDDLSMGKKENLDME
                     KQVTFIEGDVSDPQLMERIMKQYQFAYIFHLAAVASVADSVERPLETHRVNFDSALLL
                     LELVRKYQSGLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFASEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKTEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILNGMERYLKTEID"
     gene            complement(7989..>8674)
                     /locus_tag="IXH04_001709"
     CDS             complement(7989..>8674)
                     /locus_tag="IXH04_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="HAQ1852312.1"
                     /translation="FVFSFFTSYSSRFLKTNTLKRWLTALVVGLLSIIAMYYVTDITA
                     QAWAHVPHITEIKKDISDSSGTTSSNKVDLKRPDTDGGDVSNLRFGLWKSAIEIFKSK
                     PISGGSPGYYIEYAHDKLPYTLMGKDTLTAHNFIFLVMAATGGLGLLTLLTFLFIQCF
                     KTLRYCFTQPNLVKDKHFFAILMILTIAVSALFITELILVSTIGSFIFWGYLGSIQKT
                     ISKEEGVTN"
BASE COUNT         2609 a         1799 c         1342 g         2924 t
ORIGIN      
        1 aaaacttgtg tcaccctctc tttttttatt gttttttaaa gtgataggtt ggctcctcca
       61 ggttgggatc ataatcaaca agtaaatcat atcctttaaa gaaccattca tctgcttcct
      121 cgataaaaaa aagtgtccca tccatttcca ctttctttac aggatgttct ggctgttcaa
      181 ctgacattcc aatcgaaaaa ccatcatgaa cctctgtttt gccgtagact tttccaaaaa
      241 aacgtattcc gtaagtttct ggcagatcaa tttctgagtc aaaccacttt tgtgcttttg
      301 gcgttaccgt taattccatc ccaacaacct actttctctt ttctttctat tgattcatct
      361 tatcgtctag aatatccaca tcgcctgtct ctccaatcac cagtagatta tcattttcgc
      421 gaacatattc atctgctgca ggagaagcga taaccgtttg atctactctt cgtatggcga
      481 ctactgttaa gtcaaaacgt tgcctgaaat ttaattcagc taatgttttg ttgaaaaatt
      541 taggattgga gactcggatt tctgctaaag aatatttatc tgacagctct aagtagtcta
      601 gaatattctt tgatactaag ttatgtgcaa tccgtatccc catgtctcgc tcagggtgta
      661 cgacacgatc tgctccgatc ttctctagta cacgggcatg gtactcattt tgggcttttg
      721 ctaaaatatt cggtacgccc atttccttta ccatcagagt aactaagata ctggcttgga
      781 tatcttctcc aatagcgaca attacatgat cgaaatttct tatacctaat gaacgaagtg
      841 tcatctcatc ctgtgcattt gccacaacag catgagtggc tatattcata tattcattga
      901 ctcgatcttc attactgtca atagccagta cttcttgtcc agcttcgatc agtgtacggc
      961 agatgcttcc gccaaatcgt ccaaggccta caattgcaaa attttgtttc accattttcc
     1021 tcctttagta tcttcttttc tcagtataac aaagcctttc caaaaacaaa agcaagcatc
     1081 attattgtaa aaaagccaag aaaaataata gttattttct cagcttttga ttatcctctt
     1141 tatcatcgtt tttatttcac caaaccatgt ttaaacgcat agatagctgc ttgagttcgg
     1201 tcttctacct ctaacttagc taatatattc gacacatgcg ttttaactgt tttcagtgtg
     1261 ataaacaatt catccgcgat ttcctgatta cttttccctt cagaaatcag catcaaaatt
     1321 tcattttctc gattcgttaa ttcttcatgc aatactggat catttcgccg actcattttt
     1381 tccatcattt ttgttgtcac ttcaggttcc aatactcgct cgccgcgttg cgtagccctt
     1441 atagcatcag ctatttcatg tgcggtagac gtcttcaaca aatatcctgc tgctcctgct
     1501 tcaattgcag gataaacttt ttcatcatca ataaaacttg tgacgataat gattttggct
     1561 tttggacaat cttttaagat tgcttttgtt gattcgatgc catccatttc ttccatgact
     1621 agatccatta aaatgacatc tggtcgaagt gccatcgcct tttcatagcc ttgacgtcca
     1681 ttttcagctt ctccaatcac ttcaatgtct tcttggatag ataaataaga agagacgcct
     1741 aagcgtacca tttcatggtc atctactaat aaaactttta tcatctttat ctatgcctcc
     1801 ttcatcaaag ggaccttgat ctccacgctc gtaccttgcc ctttaaaact aatgattttt
     1861 actgttccac caataccagc tactcgttct ttgatattgt tcaatccata acttcccgtt
     1921 ttggtttcat tcatattaaa tcctgtcccg tcgtcaatga tacgtaataa aagattgtta
     1981 tctattttat gcaaatagac ttctaattca ttagccttag catgtcttaa tgtgtttgat
     2041 aacaattctt gaacgattcg gaacaagtga tcctcaatgg aactagttaa ttttacatct
     2101 tctacatccc attttagcga aatctggatt ttattctgca attccttcaa taactgctca
     2161 atgccttgtt ttaaactttt ttcttctaaa ttgactggac gcagatgaag aagcagcgca
     2221 cgcatctcag actgggaagc gttgatgatc tctgccacca ttttcaactg cttacggaac
     2281 atttctggtg tctcactttt ttctgcttgc tccgtcaatg ccgacatcat catcatcgct
     2341 gcaaataatt gctgtgaaac tgaatcatga agctcacgag ctaatcgatg cctttctaat
     2401 tctaaaatct cttctttggt ttgtccatct acatattgag gacggctagt cataatctgt
     2461 aattcactgg atacttcgat cattttttct tttattttag caatttccat atcaatatct
     2521 ttgatgtaca gatcatccga cgcgcttggg atcggctgat gcaaaagttt ggattcataa
     2581 ttgccagaag aaagtgcacg aagcttttct tcgattttcc cgtattttgc cttttgaata
     2641 agtgataaca atagaaatac gatcaatcct acacctaatg aaattgccat caaatggaag
     2701 ataagcggaa cgtataataa acgcgcacgc agcaattctc cccaccaatg actttggtta
     2761 ctggcataaa aataagtgaa aagtgaaaat agaataataa ggaaagccgc aatccccgaa
     2821 taaacagcta gcattgcttt ggatattctt cccatcatat tcgtatcacc tcgacatctc
     2881 caaccaaggt attggtaatg atcttcaatc tgcgaggatt ttcatcataa tcactgctgc
     2941 agattttgat ctgttcgttt tttaaggaaa actgctcttc ttcaaacaag acatttccta
     3001 ctaatgtcgc atgctctaaa cgaatagcca ctcccagggg tactaagatc cgtgttctgc
     3061 ctatcccttt tcttacaatc acaatattat catcttttgg caaaagtgta ttacccaaat
     3121 cgatgatagt atcaccagaa atgatggcaa tgttgatatc atcccattca tagacgtcgt
     3181 ttccaatacg ttggttacca aataattgct gtcttttacg ctcattatta tgtgttttta
     3241 attgctctgt ttggaccatc atgatctgtt tttttcgcca aaaagcattt tttgtcaaat
     3301 ctattcctga aatctctaca cctttcagcc cgataaataa aacggccaat acaaccatca
     3361 tccataaagc aggactgttg actaagctaa aaaaaatgac caagctccct aagatcaatt
     3421 gaaaattttg aaatttagta cgaggatatt tgcgcatagc tagatatata ttgaaaatac
     3481 cgaatattac taaaagcaaa agtccagtat tgtttacgat ttgccaaaca gcaaataaca
     3541 gcagcagtgc ttcgactacc acaaaaaaac gccaagaact attcatcgct ttttctccct
     3601 cctgacacat tctcatttca tcatacctat ttttttgact gttggctata ggactttaga
     3661 tgtaagatcg ttcagtgaaa cctgcaatac tcaaagagaa aagatctaga gtgcgtcatc
     3721 agtaagatga actctctaga tctttcagtc agactacgct tgttctactt tgacgatttt
     3781 aactaacata tcgccacctg gtgtagcaat ggttacttca tcatttttac gtttgccaat
     3841 caatgcttgc gcgataggag aatcattgga aatctttcca gaaaacgggt ctgcttcagc
     3901 acttccgaca atcgtgtact cttcttcatc tccatcaggc aactcgatga acgtaactgt
     3961 tttaccgatc gatacctcat ctgcatctac gccgtcatta tcgatgattt gtgcaaaacg
     4021 gatcatattt tcaattgttg tgattcttcc ttcaacaaat gcttgttcat ctttagctga
     4081 ttcgtattcg gagttttcgg aaagatctcc gaaaccgcga gcgattttga ttcgttcaat
     4141 gatttctccg cgtttaactg tttttagttc ttctaattct ttttctaatt tttctttacc
     4201 ttctagagtc attggataaa ctttctctac catgtgtaac gtctcctttt ttcgataaaa
     4261 taccgcaaaa ggtaatcttt tgaggaatat agtccaaaaa agattaccac gcttttctta
     4321 aaatgtaaat gtttttctaa aaatgatcaa tgactagccc ctgtttttct aagaattatt
     4381 tacatatttt tgcactagtt ccatatgttc ttcatacgtt ttagcgaaat aaacatttcc
     4441 agtgctcgta tctgctacga aataataata gtcattttct gctggttcta atactgcctt
     4501 gattgccttt tcgcttggat tgtcaaaagg accaggccca tagccggtat tggtatacaa
     4561 attgtatggt gaatctacag ctgtatcttc atacgtcacc aattctttat gttctcctaa
     4621 tgcatataaa acagagatat ctgactgaag tggcatattt gccttgattc ggttaaagaa
     4681 gacttgtgca atatttttac gatcattttc ttttacgcct tctttttcaa ctagagaagc
     4741 caaagttaaa acttcttgta cagtcaaatt tttctgtttg atagctgaat aatagttttg
     4801 catgaccgta tttgtcttgt tcaccatctc aatcacaaga tccttaagcg aagtactcgt
     4861 gtagtaatca taagtagctg ggaataagta gccttccaaa cggtattttg tgttctcagc
     4921 tttagaggca ctttccaaca attctgggaa tttttgatac aactcgttaa agaaagtctc
     4981 atcatttact aaatctaaaa attctttctt gtctttacct gttaccttcg ctacccgctc
     5041 agcaatctga tcgatgtcgt agccttctgg aatggcaatt ttcccatctg caacttttgt
     5101 tggctcagat gtgccgcctt cttgcaactg cttgccaatc tcatcaagag tcatattagg
     5161 cgctaactga taataaccag cttgaaagcc agttaagttt ttaaattttg tataataatt
     5221 gaaaacaatc ccacttttaa taatattgtc tttctccaaa atttcgccaa tctgcttatt
     5281 agaagagccg tttgggattt ccacttggac taattggtcg tccgatttat ctaaaggctt
     5341 caacccgcta tctacataac gatatacggt aaacccgaga aatccgccaa taatcaataa
     5401 tgtaagagcg atcactaaaa tgatcttcct cacgatctta tcttcttttt tacgcagatt
     5461 tctccgcttg tcgattatat ctgctgtagc tgccttactg gcttcttttt tgtctttgct
     5521 tgggtgttgt ttgtttcctg acaaaacaac tcctccttac tatcatcttc gtaactctgt
     5581 ttattataca tgaaatttct actgccgtga attattttac caaatattat gtcaaaattg
     5641 taagtggcaa atgctctttt tgaggattac aatgaaaaat agttaaaaat ggatagaaag
     5701 gatttaatat ggcaaaagga aaacatcaaa aaaaacagca gatctctctt tcttttctcg
     5761 cagttttgtt gctggctgtc agcttctttt caacaggcat cattacagga tggcaattaa
     5821 caaaacaacc tcaaccacaa atttataaaa cggaacccgc acaacttgct tccaatttaa
     5881 catctaatga gcagatgctt gccagcaaaa ttgatcaatt actgcaaaaa tcagattata
     5941 tcggttcgat ttatgtacgc aaaaataatc gagtgatatt agaaaaagga tatgggtacg
     6001 caaatctacg cataaaagaa tcgaattctc cttctcttta ctatcaaatc ggttcgatcc
     6061 aaaaagcgat gactgcttta cttatcctaa aacaaattca atctggcaag ataacttttg
     6121 acacaaagct atctgaattt tatcctcaga ttcctggaag tcagcaaatc tcaatcaaaa
     6181 atatgcttta catgcgctct ggtcttcatc gaaccgcttc tccaaaaaaa cctatgtcag
     6241 atagtgagat cattcagttt gcccttcatc atttgaaatt tactggttat gatacgtatc
     6301 attatgaacc attaaattat acattgctta caggggtatt gatcaaacta atgaatcagc
     6361 cttatgaaac tcttttaaaa aaagaaatca ttcggccact ccatctagaa catacagatt
     6421 tttatgaaaa tgtaaaaaat tcccctcagc atgctgtatc ttaccaaatg tctgcaactg
     6481 acgattattc aaaagcattg gtcgagcctg aaacagacat ccgaaatgaa ttaggcacag
     6541 gaaatataag tatgtctgtt tatgacttga acaaattctt taccagtgtt ctgtctggcg
     6601 atattatacc aaaagaacta cttttctcat tgtggcaaaa taatggtgat aaacatcctt
     6661 acagcggcgg tgtttacagt ggaaatgatt atatccttgc tcagggaaat atcaatcgat
     6721 tccatgcagt agcggcgatg aaaaaagaca caaaagatgc aatcgtcatg gaaagtaata
     6781 tccaatctga taaaaacatc aaattgccgg ccactgattt aagaaatcag atttacgaac
     6841 tgatggaagg tgttaattta aaaagctaag tcgagacaaa aaaaccagca ggatgctggt
     6901 tttttgtttc aatcgatttc tgtctttaaa taacgttcca ttccgttcaa tatatcaaat
     6961 ttgggttgat aacctaaaga acggatcttt gaaatatccg ctaaagaatc acgaatatcc
     7021 cctgaacgct ccgcttcata tttaagcgct agctctgttc ctaagatttg atcaattgaa
     7081 tgaatcaatt caagtagcgt agtgcttttt cctgtaccga cattgaattg ctgtcctaaa
     7141 gctttttctt ccttagcaac aagtaaaagt gcttgtataa catcttctat ataaacgaag
     7201 tctctagatt ggctaccatc accaaataaa gtaaattctg ttttttctcc tgccaattgt
     7261 tttttgtatc ggtccactag aatcgaaata actccgctat atggagagtt aggattttga
     7321 tttggaccat aaacgttaaa aaatcggaca gcactagtcg gaacatcata taaatgacag
     7381 taatccaaaa catattgttc tgatgcaaac ttatcgatgg catatggtgt taaaggacgg
     7441 atgaccgatt cttctttttt tggcaacgtt ggttcatcgc cataaactgc agctgatgaa
     7501 ctaaatacca atcgttttaa accagattga tattttcgta ccaattcaag caataaaagc
     7561 gcactgtcaa aatttacacg atgagtttct agaggacgtt ctacagaatc tgccacacta
     7621 gctacagcag ctaaatgaaa aatataggca aactgatact gtttcatgat tctttccatc
     7681 aattgaggat ctgatacatc cccctcaatg aaagtcactt gtttctccat atccaagttt
     7741 tctttttttc ccattgataa atcgtcgact actacgattt tgttttcttt tcccaaataa
     7801 ttcgctagcg ttgaaccgat aaaaccagct ccaccagtaa tcaatatagt atccatcaaa
     7861 ttcactccaa ttatgcattc tcatacttaa taaagttagt ttaccatttt tatattgtaa
     7921 aaactataaa atttgttgaa gttgggataa ctagttttct tttctacttt atatacctaa
     7981 aaatagtttc aattagttac tccttcttct ttagatatag ttttttggat agaacccaga
     8041 tatccccaaa aaataaatga acctatcgta ctaaccaaaa tgagttctgt gataaataat
     8101 gccgaaactg ctattgtaag aatcattaaa atagcaaaaa agtgcttatc ttttactaga
     8161 tttggttgtg tgaaacaata acgcaaagtt ttaaagcatt ggataaataa gaaggttaac
     8221 aatgtcaata gacctaatcc tcctgtagca gccataacta agaaaataaa attatgagca
     8281 gttaaagtat cttttcccat taatgtatat ggtagtttat catgtgcgta ttcaatatag
     8341 tatcctggag aaccgccgga tatcggctta ctcttgaata tttcaattgc acttttccaa
     8401 agtccaaacc ttaaattaga aacatccccg ccatcagtgt cagggcgttt taaatcaact
     8461 ttattgctgc tagttgttcc tgatgaatca gatatatctt tttttatttc tgtaatatgc
     8521 ggaacatgtg cccatgcttg agcagttata tctgttacat aatacatcgc aattatactt
     8581 agcaatccta ctactaatgc agttagccat ctttttaaag tattagtttt aagaaaacga
     8641 ctactataac ttgtaaagaa actaaaaaca aaag
//
LOCUS       DACEXQ010000108         8573 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.108, whole genome shotgun sequence.
ACCESSION   DACEXQ010000108 DACEXQ010000000
VERSION     DACEXQ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.108"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(132..1085)
                     /gene="menA"
                     /locus_tag="IXH04_001710"
     CDS             complement(132..1085)
                     /gene="menA"
                     /locus_tag="IXH04_001710"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAQ1852313.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1098..2201)
                     /locus_tag="IXH04_001711"
     CDS             complement(1098..2201)
                     /locus_tag="IXH04_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAQ1852314.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(2291..3226)
                     /locus_tag="IXH04_001712"
     CDS             complement(2291..3226)
                     /locus_tag="IXH04_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAQ1852315.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            3528..5462
                     /locus_tag="IXH04_001713"
     CDS             3528..5462
                     /locus_tag="IXH04_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1852316.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5919..6335
                     /locus_tag="IXH04_001714"
     CDS             5919..6335
                     /locus_tag="IXH04_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAQ1852317.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            6349..6915
                     /locus_tag="IXH04_001715"
     CDS             6349..6915
                     /locus_tag="IXH04_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAQ1852318.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            6938..7924
                     /locus_tag="IXH04_001716"
     CDS             6938..7924
                     /locus_tag="IXH04_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ1852319.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(8079..>8573)
                     /gene="scm"
                     /locus_tag="IXH04_001717"
     CDS             complement(8079..>8573)
                     /gene="scm"
                     /locus_tag="IXH04_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAQ1852320.1"
                     /translation="TSTTSDSTDTSASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSD
                     SSDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSK
                     TSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYK
                     KSKR"
BASE COUNT         2519 a         1653 c         1744 g         2657 t
ORIGIN      
        1 tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatat
       61 aaggagctat ttttatttgg ctgttcttat tacttgatgc agggcagttt tttcacaacc
      121 tcttcatgct gttaaatggc ttgcataatg attgttagta atagccccaa tacataactg
      181 gcattgaata agaccatatt tttgatggaa tagctgaaac tgataggctg gggtaacgtt
      241 tgcttgaatt gatccaaatg cttcttcaca attggcaagg tcaagaaggt gaccaagatc
      301 ggccattcat agataccgct caacaacccc attaaaatca cgaaatagca agcatacatc
      361 aatagctgga ataagactgt tcccgcagga cgtccgatat aaaaaactag tgtatagcga
      421 tgattttcaa tatcacggtc aagatcccgc aaattattag ccaacatgat attggcaatc
      481 gtaaacacca agggtaagga agctaatacg attgctgcta tagcccaaag atttcctacc
      541 aaatcaaatt gtccagatga aaagttgact gtcagatcat agacatctga accgaatgta
      601 ttgacataaa cgaccatggc aaaaattcca aggcccatag tcaccccact gaaaatttcc
      661 cctaaaggca ttcgagataa aggaatcggt ccgaatgtat agaatactcc aataaaacaa
      721 cagataaatc ccatcaaaag taaaagccaa cccgtattga tcgttagaat aacgccgata
      781 acaaaaacaa atgcaatcat agctaaaatc atgtttcgaa tcaatacagg agatagattt
      841 ctgcggccaa tgacattttc ttcatactta tatacttcag aatgagcctt ttcataatcc
      901 atgtaattat tgatagcggt agtcgctaaa tcaaaaacta gcattccaat aaaaaataaa
      961 aaagtgttta gccaatgtat ctcatgaaaa aaagcgatag agaataatac ccctaccgca
     1021 aaaggaaata cactagccac ctttgttcgt atttcaacta cttctaaaaa gatttttagt
     1081 gtcatggttc gtactcctta ttttagttca gaacggtcac ccatcgtgta atgggagtcc
     1141 tccgataatt tgaaggtgtc tttgattgga tcggtcacat atactttatc gtcttttgtc
     1201 acgaagatcg cttccgtccc gttattcaat tcgctttcga cgtattccag tccttttttc
     1261 acacccattg aaaagactgc tgtagacaac ccgtcaccat caattgattt atctgttatg
     1321 atcgtgacac ttgcgatatc attgtcgaac ggataacccg tttttggatc aaacaaatga
     1381 tggtattttt tgccatctac ttctaaataa cgctcgtaaa ttccagaggt aactaatgtt
     1441 ttgttccgtt ctttaataga acctaaaaca gatccgcgag ccatgttcgg atcttggatg
     1501 ccgactgtcc agtcttggtt ttcgcctctt gggctgtggc ctaagacata gacattaccg
     1561 cccaagtcaa cgatggcagt ggtcactcct tgttttttca agactttcac tacttcgtcc
     1621 gtaatgtaac ctttggcgat tgcacctaaa tcgatgatca ttcctttttc ttttagataa
     1681 acagtttttt ctttgtcatt tagttcgatc ttatgataat ccactaattt caatgcctga
     1741 tcgatttctt cttgggaagg ttttctggcg tcatcaaaac cgattcgcca taattgtgtg
     1801 attgcaccaa tcgccatatc gaatccgcct tgagaatctt ggctatattc gtaggcccgc
     1861 ttgactaacg tgtagacgtc atcagaaact tttactggtt tgatcccagc ttgctcattc
     1921 acttcatcga tctcagaacc tttttgattg atcgtgattt tatcaccaag ttcttttact
     1981 cgatcgaagg caggtttcaa tgctgattct ttgccttcat catacacacg gatacgaacg
     2041 taagtcccta acaagaactg ttcatcagaa tatggatctt tcaatatctt ggtagctgct
     2101 gaactttcag tggttgtttt atttgctgag ttgctgcagc cagttagcac cataaaagag
     2161 atgcccagtg tgattaagga aaatagtagt tttttccgca tctttttcct ctcttcattt
     2221 tgaaaggtga gacgactgcc taatctggct atcgtcccac cctccaactt ttattagtat
     2281 gattatgtga ttatttttct gttacgatat tatctacttc gattgtttga gtgtcgcctt
     2341 tttcagctgc attcactaat tgttgtgcgt acatgatgaa tgaatgtgag ctgtgtgtag
     2401 cacctgtaac aacttctaca ttagcaggtg agccatcttc ttcgcccatt gctttgacga
     2461 attcgtcgtt cagagtaggg atgtaagttt ttggttctgt accgcttttt gctttcatat
     2521 tttcattata ttctgtatca tcttgttttg atttaccgtc tttgtcgata taatcaaagt
     2581 ttgattcagt tactttgccg ccagcaactg tcatttcgaa ttgcacacgg tagccgttag
     2641 agtagttctt ttcttcaagc ttgtaagtgc cgtctttcaa atcagcacca ttgtcgattt
     2701 cgattgtatc tgtgttacca gcttgagcag cttggatcaa ttgttgtgcg tagttttgga
     2761 agctttcaga tgagtgagta gctccagtta cgacttccac accgcttgcg ctttgtgctt
     2821 ttaagaattg gtcattcaac tcaggaatgt atttttctgg gtttgtgcca gattttgctt
     2881 ccatgttttt gttgtattct gcatcttttg tttttgattc gccgttttca ttgacgttat
     2941 catattttga ttcagtgatt ttgccgtctt ttacaaccat ttcaaagact gcacggtaac
     3001 catttgaata atttttttct tctaatttat atgtgccgtc ttgcaaatct ccgccagcta
     3061 caacttttgc agatgattct gttgtgcttt ttgcagtcga agattgtgct gtagatgatt
     3121 cagaagaaga gtttgagctt ccgttgtcat ctgcgccgca agcgcctaac actagagctg
     3181 aaaatgctaa tacggcaaat ccagttacaa aacgtttttt cttcatttta ataccccacc
     3241 attccatttc tagtccacaa tttttctttg tgattcatta tacattctaa tctttttagc
     3301 ctataagtca actatttttg ataaataacc catgtaatat ttcaaacgtt ttctttcagc
     3361 aaatttcgtg aaaaaacgct ttattttaaa atttgaaaaa aatccaaaag gagttagaca
     3421 aaagaacaga acatctatat aatatatgga gattgtgtaa taagtatcaa actaaaagaa
     3481 ttatcatata ttcattatat agatagtcta taaggagcgg atgatcaatg gcaaagcaaa
     3541 atattgtcgt tgtaggagca ggatatgccg gtgtttcagc tacaaaatac ttggcaaaga
     3601 aactgaaaaa agaagatgta gcaattactc tgatcgatcg tcattcgtac catactatga
     3661 tgaccgagtt gcatgaagta gctggcggac gtgtagaacc agaagcaatc caatacgatc
     3721 tgcagcattt gtttgctcgt caaaagaatg tacaacttgt cacagacact gtaatcggta
     3781 tcgataaaga acgaaaaatc gtcaagacca aattaggttc ctatcctttt gatcagctga
     3841 tccttggaat gggcggagag ccgaatgact ttggtacacc tggtgtcaaa gcacacggat
     3901 tcaccctttg gtcatttgaa gattctgtaa ggattcggga acatattgag aagacagtag
     3961 caaaagctgc attggaacca gatgatgaac tgcgtaaagc catgctgact ttcgttgtct
     4021 gtggttctgg atttaccggt attgaaatga ttggggaact gatcgactgg aaaaatcgcc
     4081 tagccaaaga ttataaatta gatccaaacg aaatcacact gatggtcgta gaggcaatgc
     4141 cgacgatctt gaatatgttg gatcgaaatg atgctgcaaa agctgaacgc tatttgcgta
     4201 agcacaacgt ggaattatta ttgaatgcac caatcgtaga agtggctgct gatcatatca
     4261 aattgaaaga cggacgcaac attccgactc acacactgat ttggacagct ggtgtcaaag
     4321 gaacttctga cgctgctgac tttggattag aatcagcacg cggacagcgg ttgatcgcca
     4381 atgaatatat gcaggcaaaa ggctacgaag ataaaaacat ctatatcatt ggtgatttag
     4441 tcttttatga agaattccca ggaacaccga caccgcagat cgttcaagct gctgaacaaa
     4501 caggacacac agccgcagcg aatatcgtgg cttcgatcaa aggcggagaa aagcataaat
     4561 ttaaaggaaa ctatcaagga tttatggttt ctattggtgc caaatggggg gtagctaacc
     4621 tatttgataa gatccattta agcggatttt tagctatcgt aatgaaacat atcgtgaatt
     4681 tgaaatactt ctttgatatt cgttctggtt attatatgtt ccaatattta atgcatgagt
     4741 ttttccatat caaagatgat cgcaacgtga ctcgaggaca ttcttctcgt tacggcaatg
     4801 tcttatggag tgtgccactt cgtattttct atggtttagt ttggttcgtt gaagcttcca
     4861 aaaaaatcat tgggaatggt gaagtgttca aaccaagtac ttggtttggt gaaggatcat
     4921 ggttcactgg aaacagtgtg ttcccttgga catgggaata caatgtaaca gatgttgcca
     4981 gtggtgcttc tgctgctggt gatgcaacca cagctgccag cggagcaggg gcaagtgccg
     5041 gagaagcagc gactcaagct gcacattttg gtttgagtta tgcatacggt gaagaaccaa
     5101 tggctgtgat tgagaagtca ccagactggt tcacaaagat catgcaagtg atcatgccaa
     5161 acaatgatgt agcaatgttc ttccaaaaat tcatggtatt ctttgaaatt gccttggcat
     5221 tagcattgat tgctggatta ttcacatggc tatgcagtgc gacaacgatt gtattggtga
     5281 tcacgttctg cttatcaggc atgttttact gggtaaacat ttggttcatt tttgttgctt
     5341 tcgcattgat gaacggttcc ggacgtgcga ttgggttgga tcgctgggta attccatggc
     5401 tacaacgtac actaggtaat tggtggtacg gtagaccaag ttcccagtac ggcaagaaat
     5461 aaatagataa agaacgttat ttgaatgcaa gatttcaata gaaaggggct gagacaaacc
     5521 gtcacagcct cctttttatg taaaattaaa ggaataagat gcccgagata agcctccatg
     5581 agcaagacac aggtgtcaca acctctgtta caacctcttg ttataaacgg tgttccagaa
     5641 gtagcttctt cgaaaaataa gccgaaattt cacaaaaact tgaaaagtaa ttttcgtaaa
     5701 ttccttctta tttcttgaag ttgcccactt ctgtcacaac ctcttgttat aaacggtgtt
     5761 ccacctgctg cttctcggta ataagcccaa agaattagaa aacatggaaa gctgttttct
     5821 attcttcgtt cttattactc aaagcaagac acaggtgtca caacctcttg tttttatgct
     5881 aaaattgggt gagttaatct ggaaaaagga gaacaaaaat gagccttaaa gaatttgctg
     5941 aaaaaagtct gctcaaacct tgggatgttt ttattatttt gcttttgact gttctttctt
     6001 ttttaccagt catgattttt ggtcttcagc aaaacaccgc aacagcagaa aaagaagcgg
     6061 tactgcgtgt ggatggcaca gaaatcaaaa cattcccttt gatggctgga gaagagagca
     6121 acacatatac ttacaaagat gaacacggag attataactt gatcgagata gatggcgatc
     6181 gagtacggat aaaagaagca gattgcgatg accagatctg cgtacgtcga ggctgggcaa
     6241 cgaaaaatgg cgaaaccatc gtctgtttgc cacataaatt agtgatcgaa gtccgttcaa
     6301 cagatggagg tgacgaagac agtctgattt attagacctt cacacaccat gagtaaatta
     6361 caaaaaatga tttatatttc gctacttgtt gcacaaggag tgatcattgg tttattggaa
     6421 aatatgatcc cttatccttt ttcttttgcg cctggtgcaa aactaggctt agccaatcta
     6481 atcacgatcg ttgccatttt taccatgaaa aaacaagaca gcttcttact actttggcta
     6541 cgactattcc tgactacttt gctaggcgga acgatttcca cattccttta tagtatgagt
     6601 ggtgctttgc ttagttattt tggaatgtta ttggtcaagc aattaggacc aaaacgtgtc
     6661 agtatcatcg gaattagtgc ggccggcggt ttcatgcata atgtcggaca gcttttgatg
     6721 gcttctttca ttgcacaatc atggacagtc atgttgtact tgccagtcct ttcagtttta
     6781 gggattttat cagggattgc aatcgggatc gcagcgaatt atcttttacg acatgtgcag
     6841 acacttcaac gattccaaaa agattatgag cagggaacca aacaaaaatg gagcccaatg
     6901 tttaccaaaa aataatcgtc acacaaggag tttttttatg aagctacatc caatgtggga
     6961 aaactatcct gctttaagta aagaattgac agaaacacta aaacggatgg aagaaactgt
     7021 ccgcctgaaa aacaaacctg tcgaacaagc catcaaagaa acgatccatg ccggcggaaa
     7081 gctgttgcgt cctgcctatc agctattgtt ctcacaattt ggaccagagc aagatcgaga
     7141 caaagccatc gctttggcag cttctatcga aatgctccat acggccacac tgatccatga
     7201 tgatatcgtt gatgaagcgg atctgcgacg tggtcagcct aatatccgta gtcagtttgg
     7261 taatgtcgtt tctgtctatg caggagacta tttatttgtc tgctgtttta aactattgtc
     7321 tgattactcc acttccttga aaagtctcca attgaattct cgcagtatgg aaaaagtatt
     7381 gaatggcgaa ttagggcaga tggatgatcg ctataattat gaactttctg taaaagaata
     7441 tttggaaaat atttcaggta agacagctga actgttccaa ttaagttgtt ctgttggcgc
     7501 ttttgaaagt ggaacaagcg agcgcttcgc aaaaaaagca ggagatatcg gactttctat
     7561 cgggatggcc tttcagatta tcgatgatat tttagattat acaaaagaga gccaggaaat
     7621 cggcaaacct gtactagaag acatgcgtca aggggtctat tctttaccgt tgatctactc
     7681 tttgcaaaaa aacaaaccaa agctactacc ttatttagag aagaaagcgg caatgacaga
     7741 agaagatgtc gacgccgtac gcaaaatcgt tgaacataca ggaggcgtgg aagaagctag
     7801 gaagttagcg gcctcttaca cagaaaaggc tttgaaggaa ataaagaaat tacctgccac
     7861 ctctcttcga acaaaagaga atctcttttc ccttacccaa ctgattttgg atcgcaaaga
     7921 ctgaataggg aaaggttcga tacgtaaata aatttctcag aaggaaatct acgtgttttt
     7981 tgacaaattg aattgtagtt cttgtattta tgataaaaag caaaaataaa gagcaacgtc
     8041 cgttagcgac gttgctcttt tttatcttga atattttttt accttttcga tttcttataa
     8101 ctcataaaag tgattcttaa accgattaca agtaatataa cgcctgcgag tgttatccaa
     8161 ttattcgatt gacttccagt tttaggaagt gctctttttg atttgtttcc attgtcattg
     8221 cttgttttat tagttaagtt aaagttcgtc ctgttatccg aacttgtttt tgagaaggag
     8281 acacttgcat gacgtgtatc gcttgtggta gacgacgtgt ctgtactatc gcttgaagtt
     8341 gatgaatctg agctatcact tgtggtagag gatgtatctg tactgtcact tgaagctgac
     8401 gtgtccgtgc tatcacttgt agtggtagat gaatctgagc tatcacttgt ggtagaggta
     8461 gtatctgtac tgtcgcttga agctgacgtg tccgtgctat cacttgttgt ggtagatgta
     8521 tccgtactgt cgcttgaagc tgacgtgtcc gtgctatcac ttgtggtaga ggt
//
LOCUS       DACEXQ010000109         8542 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.109, whole genome shotgun sequence.
ACCESSION   DACEXQ010000109 DACEXQ010000000
VERSION     DACEXQ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8542
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.109"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..288)
                     /locus_tag="IXH04_001718"
     CDS             complement(<1..288)
                     /locus_tag="IXH04_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HAQ1852321.1"
                     /translation="MGVLLFLVGIVLIIMGSSKLSEAKKLKDKTSALESKVKEYSQEL
                     NARETFISEKEKNISKLNKNLSTLENKIDILNAESQRLDDEINQKYDTSLVT"
     gene            complement(375..1079)
                     /locus_tag="IXH04_001719"
     CDS             complement(375..1079)
                     /locus_tag="IXH04_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HAQ1852322.1"
                     /translation="MFAENLKFLREKYNIDQQELAEKLGRKSSSSISEWEKGKYTPKL
                     KTLNEIAKIFNVDIDDLMNIDLSISNQIYNILPIYNQLNDENKVATYEFASNRLEEQK
                     QENVLDFPKKEKIPTVHNSASAANPTELTYGDTVVEEEEFERVPSAADFAVPIIGDSM
                     EPVIRNGQFVFVKEQPDVEDGEIAIVELGGDGVTCKEIYKDYENQTIILRSINDLYED
                     RIVSPEQIRIIGKVVF"
     gene            1279..1545
                     /locus_tag="IXH04_001720"
     CDS             1279..1545
                     /locus_tag="IXH04_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1852323.1"
                     /translation="MDTAKKEKYTLRQLRALKGYSKEELSRKSKVTSRTIFIYENDVD
                     KMRNGKYATLEKIAKALGVRVSDIFLDPDSEKPKSITGETKQVS"
     gene            complement(1713..1943)
                     /locus_tag="IXH04_001721"
     CDS             complement(1713..1943)
                     /locus_tag="IXH04_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ1852324.1"
                     /translation="MGKNQWVSPRNGKWAVRGEGNSEDTKLFDNKSDAVEYGKKISKK
                     QQSEFIVQKKNGQIQSKDSFGNDPIPPRDKEH"
     gene            2015..2170
                     /locus_tag="IXH04_001722"
     CDS             2015..2170
                     /locus_tag="IXH04_001722"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852325.1"
                     /translation="MSNKPKITLNGTPNLQRLADEMARIRSRHWGVTVVATIIEQKEK
                     PTQPASK"
     gene            complement(2479..2703)
                     /locus_tag="IXH04_001723"
     CDS             complement(2479..2703)
                     /locus_tag="IXH04_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004401476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ1852326.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            2970..3305
                     /locus_tag="IXH04_001724"
     CDS             2970..3305
                     /locus_tag="IXH04_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852327.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            3538..4479
                     /locus_tag="IXH04_001725"
     CDS             3538..4479
                     /locus_tag="IXH04_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAQ1852328.1"
                     /translation="MSKSTLEMSHQEWLEDRKRGIGGSDVATVLGLNKYKSPYQLWLE
                     KTGQIELKDLESEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPVIDGSGATADFLKEKYADVEENQTA
                     LPSRFDELIEQKRELKRTKKEIESSIRQVDNEIISELGKREASIGITQRNIISWKLVR
                     TRRMNTKKLAEKYPDVANDEEIYNVTESRRLTEKEIK"
     gene            4481..5371
                     /locus_tag="IXH04_001726"
     CDS             4481..5371
                     /locus_tag="IXH04_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HAQ1852329.1"
                     /translation="MATNESLKNQLAEKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            5414..6214
                     /locus_tag="IXH04_001727"
     CDS             5414..6214
                     /locus_tag="IXH04_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAQ1852330.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWSLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADNIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            6229..7080
                     /locus_tag="IXH04_001728"
     CDS             6229..7080
                     /locus_tag="IXH04_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ1852331.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGSIA"
     gene            7077..7436
                     /locus_tag="IXH04_001729"
     CDS             7077..7436
                     /locus_tag="IXH04_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HAQ1852332.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELEGKKYRKIEYIPDFTFYKSGKLVKVVDVKGMQTKDFKIKAKLFCHQYQV
                     PLILAKKYRNTFKEERF"
     gene            7451..7612
                     /locus_tag="IXH04_001730"
     CDS             7451..7612
                     /locus_tag="IXH04_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ1852333.1"
                     /translation="MTTEEVIQMRIRSLRREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            7609..7935
                     /locus_tag="IXH04_001731"
     CDS             7609..7935
                     /locus_tag="IXH04_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852334.1"
                     /translation="MSVDKEAAWRKLMLLIQDENWQEDEAVVAEVQRLGKIANGRIRK
                     KPDKRKQRKGKIVVVLHEGKILMQGTASELSAETGYTRGTIRTYAWRNHTDRKGHEYK
                     YLEEEK"
     gene            7932..>8542
                     /locus_tag="IXH04_001732"
     CDS             7932..>8542
                     /locus_tag="IXH04_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HAQ1852335.1"
                     /translation="MNENKLIKLGVAGAVIVGIGVIGGFKFFEKIDNGYVGVRYSMNG
                     GIKDEALTQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTK
                     AAKMYKEFGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFA
                     KSVESKGFEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKL"
BASE COUNT         3025 a         1408 c         1784 g         2325 t
ORIGIN      
        1 agtgaccaga gatgtgtcat atttttgatt aatttcatcg tctaaccgct ggctttcagc
       61 attaagaata tcaattttgt tttctagcgt tgacaagttt ttattcaatt tacttatgtt
      121 tttctctttt tcagatataa acgtttccct agcatttaat tcttgagaat attcctttac
      181 cttactctct aaggcagaag ttttatcttt taatttttta gcttcagata atttgctact
      241 acccataatt attaaaacta ttccaactaa aaacaatagt actcccattc aaaacgcctc
      301 gcttctttta taaaataatt gtcaaaatca tataaaccct taccgaagtt gacggctccg
      361 gcatgggaat ttttttaaaa tacaactttt ccgataattc taatctgttc tggcgataca
      421 attctatctt cgtataagtc gtttatagaa cgtaaaatga ttgtttgatt ttcgtaatct
      481 ttatatatct ctttacatgt aactccgtca ccacctaatt ccacaatagc aatttcccca
      541 tcttctacgt ccggttgttc tttaacgaat acgaattgtc cattcctgat aacaggttcc
      601 attgaatcgc ctataatagg tactgcgaag tcagcagcac ttggaactct ctcaaattct
      661 tcctcttcga caaccgtatc accgtaagta agttctgttg gattggcagc cgaagccgaa
      721 ttgtgaacag ttgggatttt ttctttttta ggaaaatcta ggacattttc ttgtttttgt
      781 tcttctaatc gattacttgc aaattcatag gtagcaactt tattttcgtc gttaagctga
      841 ttatatatag gaaggatatt atatatctga ttagatatag ataaatcaat attcatcaaa
      901 tcatctatat caacattgaa tatcttagct atctcattta aggtttttaa ttttggtgta
      961 tattttcctt tttcccattc gcttattgag gatgaacttt ttcttcctag tttttcggct
     1021 agttcttgtt gatcaatatt atacttttct ctaagaaact ttagattttc agcgaacata
     1081 gtaacacctc cttgagttaa ttgtaacacc gtttcggaaa aaatgtaaca ggtttcttag
     1141 aaaatatttt agtgttttta aagaagtatt tacgcaaaat agaaatcgat ttcaggaaaa
     1201 ctgaaatatg cacttgactt cggaaaaacc gaagtatata atttagtcag aaagttaaag
     1261 agaggaggcg aataaaatat ggataccgcg aagaaagaaa aatatacatt gagacaatta
     1321 agagctttaa agggttattc aaaagaagaa ctgtcaagga aaagcaaagt gacatcaaga
     1381 acaatattta tttacgaaaa cgatgtagat aaaatgagga acggaaagta cgccactcta
     1441 gaaaaaattg ctaaggcatt gggtgttaga gtttccgata tttttttaga ccctgattcg
     1501 gaaaaaccga aatcaataac aggtgaaaca aaacaagtca gctaggaggt gtgaatagtg
     1561 gaagagaaga atataaaagt accgactctt gaagaatata caaaaattgt tgtcgaaaca
     1621 gacgagcaga acccgaaaac aatcgcagtt attacggcga atgatattga atcatgcgaa
     1681 ggttttcgag ttcgaatgac ccctagatat gattaatgtt ctttatcacg tggaggtata
     1741 ggatcgtttc caaaactatc ttttgattgt atttgtccat tctttttttg gacaataaac
     1801 tctgattgct gttttttaga aatcttttta ccatactcaa cagcatctga tttattatca
     1861 aatagttttg tatcttcgga attaccttca ccacgtacag cccatttgcc atttctagga
     1921 gatacccatt gatttttacc cataacaatt cacctcgctt tcaagttaat tctattaaga
     1981 ggtgaaaaga aaacaaactg atggaggtga gattgtgagt aacaagccaa aaatcacttt
     2041 gaatggcaca cctaatcttc aaagattagc agatgaaatg gctagaatac gttctagaca
     2101 ttggggagtt actgtggtgg caaccataat tgaacaaaaa gaaaagccca cacagccggc
     2161 aagcaagtag tgggcaccat agaacaataa tattatatca cacactcgcc acttccgaaa
     2221 agaggagttg ctgctctcct ttcaagtttg ccaaagtcca cgtttcttca ttttggcaac
     2281 tcctcttctt ggaggtggtg acggcgagag acgaaggagg gatgacatga aggaagaatc
     2341 aaaaataggg attttgaatg agaacaatta cacaagatta attattgaaa cagaagaagg
     2401 ggaaaaagta gcagaagtta cattgaccga tgcaactcct gctgacggtt atcgtattcg
     2461 attaactcct aaatatgatt aaccttttgg tgggtgtgga tcgtttccgt gactatcacg
     2521 gctttgaatt tttccatctc tgccatgaat aaatagctca gatccttgat ttttagaaat
     2581 ctctctagcg atttttactg cttctgattt tgtattagta tgtgcagtcg ctttagaatt
     2641 tccagcacct ttgacattcc aaccaccatt ggaattaggt acaacatgtt gattttttcc
     2701 catagttttc acctccattt ctatagaaat attttatcaa atagaaatgg agaaattcaa
     2761 taaacgaaag gagggatcaa atgacattac aagagcatca acacttgatg cgaaaactaa
     2821 atcaggaata ccacgaggaa atgaaaccta gattgctagg ggaacaatgg cgtgaacgcc
     2881 agaagaagtg gttagaaata aaaaagtgac tcagccgtcc aaagcaatga gtcacaaacc
     2941 aaaaattata cctaaggaga ttttatcaca tgaataagaa aattgaaaat cttatcgaag
     3001 aattgaaacg tgaatgccaa aagcaaggtg tttctattat ttgtacagcc caaaaagaag
     3061 gggaacttaa aagtctcgtt tatggtgaga caacagaaat tttactttgt cttgctatgc
     3121 aagaagaaca tttagatgaa aatctcccat tgtcagcaca tattatgcgt agaattgctg
     3181 tggatgctta cgaacaagcg aaaaatgaag aagaaaatca accttcaaat catacttttg
     3241 ttattaataa caaggaagat ttagctgatg tgatgactcg tattctcaag ggggaatttc
     3301 aatgatgaat gaaaaaacaa ttgatctact cattatttta tttttcacgt tagtgattgt
     3361 agcgaaaagt tatgtaatct ttggcttgtt attcgcaatt accatgcttg tgctttgcgt
     3421 gttagcaagc agaaaagagg ttaaacatga acgagttagc aaatcttgac aactatttaa
     3481 ctgatcctga ttatgctaaa ccaccatatg aagcgccaat tgatgaggag gatgaagatg
     3541 agtaaatcta ccttagaaat gagccatcaa gaatggctcg aagaccgcaa aagaggtatt
     3601 ggtggctcag atgttgcaac cgtacttgga ttaaacaaat acaaatctcc ttatcaattg
     3661 tggcttgaga aaacgggtca aattgaattg aaagatttag aaagcgaacc agcttattgg
     3721 ggtaatgttt tagaagaagt ggttgctaaa gagtttcagg aacgtacagg caaaaaagta
     3781 cgcagaagaa accaagtatt tgaacatcca ttacatccat ttttaagagc aaatattgat
     3841 cgggaagtag tgggagaaaa tgccattctg gaatgtaaaa cagccaatca atttctcgga
     3901 aaagagtggg aaggtgaaga ggtaccgctt agctatctct gtcaagttca gcattacatg
     3961 aacgttttaa acaaagacta ttgttatatc gctgttttga tcggtggaca aaaattcatt
     4021 tggaagcgga ttgaacgaga tcaagagctg atcgatacaa tcactgaaca attagtagag
     4081 ttttgggaaa cgaacgttct tggaggtatc gagcctgtaa ttgacggaag tggagcgact
     4141 gctgacttct taaaagaaaa atatgcagat gtagaagaaa atcaaacagc tctaccatca
     4201 cgttttgatg aacttatcga gcaaaaaaga gaactcaagc ggacgaaaaa agaaattgaa
     4261 tcatctattc gtcaagtaga caacgagatc atcagtgagc taggtaaacg tgaagccagt
     4321 atcggtatca cacaaagaaa cattatcagc tggaaacttg ttcgtacaag acggatgaac
     4381 acgaagaaac tagcagagaa atatccagat gtcgcaaatg atgaagagat ttataacgtt
     4441 actgaatcaa gaaggctaac cgaaaaggag atcaaataat atggcaacaa atgaatcgtt
     4501 aaaaaatcaa ttggcagaaa agccacagaa acaagttgca ccaggacagt tagggcttaa
     4561 agctctaatg aatacaccaa caatgagaaa gaaatttgaa gaagtacttc atgacaatgc
     4621 taatgctttt atgtcgaatg ttatgactct tgtatctaat gacagttatc ttgcagatag
     4681 tgaaccgatg tctatcatga gtggtgcgtt aactgctgca acattaaatc ttgggctaga
     4741 taagaattta ggttatgcat atttagttcc attcaatagt aaaaacaagc aaacaggaaa
     4801 atgggaaaag aaagctcaat tcatgcttgg ctataaagga tatatccaat tagcccaacg
     4861 atcaggtaaa tacaaagcat taaatgtgat cgaagtttac gaaggagaac taaaaagctg
     4921 gaaccgactg acagaagagt ttgagtttga tccaaatggt agaacgtctg atgaagtcat
     4981 tggatatgtt ggctatttcg agttactgaa tggattcaaa aaaactgtct attggaccaa
     5041 acaagaaatc gaagctcatc ggattgctaa caataaagat cgcgataaga caaagttaag
     5101 tggtgtgtgg gcatctgatt acaatgcaat ggcacgaaaa actgttttga gaaatcttct
     5161 ttctaaatgg ggaatcttgt ccatcgaaat gcaagaagcc accacatcgg atgagagagt
     5221 ccaaagggtt caagaagacg gcagcattat tgctgaaaca gaagttgaag aagatattcc
     5281 tgaaagaaaa gaagcagagg ttatatctga agaaaatgaa gatgtacaaa ctggattatt
     5341 tgatgcatct aatccgccgt taaacaaata atgagggagc tttctccctt gcttttctag
     5401 aggagaaata cgaatgaaca caggatatgt caaattacat cgaaaagtga tgaattcatt
     5461 cgtttggacc aatccttata tgtataaatt atggagcttg tgtttgatga aagcgagtca
     5521 tgagaatcgc aagatacttt ttaatggaaa agagatacaa gtgaacagcg gagaattcgt
     5581 cacagggcgc gatgctatca catctgagat gaacgaaggc gtgaaacgtg aacatcaagt
     5641 gaacagcggt tctgtatgga gatggctaaa acaatttgaa aaaaacggaa tgttgaacat
     5701 caaatcaact acgaaataca gcgttatatc aataaaaaac tggtctttgt accaaagcag
     5761 tgaacaacaa atgaacatca aacgcacaac aagtgaacaa caagtgcaca caaacaagaa
     5821 tgaaaagaat tataagaatg aaaagaataa taaaacatca tcgtcgcaac cacgcaaaaa
     5881 gcgtgtttac gacaccgact cagtttacta cattctcgcg gaggagttat tcaaacagat
     5941 ttgtcagaat caggaaatca aaaagccgaa tctgcaaagt tgggcagata acattcggaa
     6001 aatgatcgag atcgacaagc ggaccgaaaa ccaggtacgg ggaatgattg aatggagtca
     6061 gcacaatgtg ttttgggcat cgaatatttt atctgctaaa aaattgcgag aaaaatacga
     6121 cacaatggca gcgcaagcaa atcgtgatta taaaacaaaa caaactaaaa cgcttgaata
     6181 cgagaaattt agcacagatg agttgcctat ttgagaggag gcgtaggcat ggaaactgtt
     6241 ggggaaatca tggaaaagct gatacagaaa gtgcttgtcc aacgtggcga atgtcctgaa
     6301 tgcggacagc ctttgtatgg atggcgtacg aagaaccctg atggctcaga acgttgtaag
     6361 ccaacctgta tgcaatgtgg gtataaagca ttgcgagttc aggaagactt acaaacagaa
     6421 cgaatttaca acgagagcct gaaagcaaga gcaatcaatt ttttcaaagg tggttctgtt
     6481 gtgcctaatc aagcgttgtt tgattgcaca ttgcagaatt atcaaattgt cgatcaagaa
     6541 acaagacaag cggttgaagt aaccaaacgc tttgttaatt cagtcttgtt aggaaatcca
     6601 agtcaccttg ttttaactgg aaaacaagga acaggtaaaa gtcacctagc gatggcagcg
     6661 gcttgggaag tcttgaagcg atcaaactac gataaaaaaa tcttatttat cgggttacag
     6721 gaaatgctgg atcaaatcaa attttcgtac aacaatcctg aactcagaaa aacgattgag
     6781 ggatcgttga ttgcagatat caaaacagcg gatttagtca tcattgatga tatcggttca
     6841 gaactaggaa aagatgcatc agatagtcga gcgtttggca taaacacgct aaattcgttc
     6901 ttggatgcac gacagaacct agcaacgatt atcacaacga acttgcttgg tgaagaactg
     6961 aaaaaagctt acggtacgag aacaatatca agaatgtttg tcaactctga tggatttacg
     7021 atggtatttt ctcaaacagc agacaagcgc ataaaaccag tgaaaggtag tatcgcatga
     7081 ataaataccg taataaaaaa actgttcatc gaggtatcaa gtttgattct atcgcggaag
     7141 cagagtatta cgatctagcc ttgtggcaag ctgaagcgaa tggttggaaa gtgaagctcc
     7201 aggaaaggtt tgagctgatg ccgaagtttg aactagaggg caagaagtat cgcaagatcg
     7261 agtatattcc cgacttcaca ttttacaaaa gcggcaagct ggtcaaagtc gtagatgtta
     7321 aggggatgca gacaaaagac tttaagatca aggcaaagtt gttctgtcat caatatcaag
     7381 tgccgttgat attagccaaa aaatatcgga atacgttcaa ggaagagcgt ttttaacgag
     7441 gtggtccatc atgacaacag aagaagtgat tcaaatgcga attcgaagcc ttcggcgtga
     7501 gattgacgat ctggaacgaa caaaggcagt gatggtcaat gaaacggcta gaaaggcaat
     7561 cgatttgcac atagagaatt taagaaggga aatccgtaga ttggaggaat gagcgtggat
     7621 aaggaagcgg cttggcgaaa attaatgttg ctgattcaag atgagaactg gcaagaagat
     7681 gaagcagtgg ttgctgaagt tcagcgtcta ggaaagattg ctaacggacg tatacgaaaa
     7741 aagccagaca aaagaaaaca gcgcaaaggg aaaatcgtcg ttgttttaca cgaaggcaaa
     7801 attttgatgc aaggaacagc tagtgagctg tctgcagaaa ctggatatac gcgtgggact
     7861 attcgaacgt acgcttggcg aaatcacacc gatcgaaaag ggcacgaata taagtatttg
     7921 gaggaagaaa aatgaacgaa aacaaattaa tcaaattggg tgtagcagga gcagtaatcg
     7981 taggtattgg agttatcgga ggatttaagt tcttcgaaaa aatcgataat ggatatgtgg
     8041 gtgtgcgcta ttcaatgaac ggcggtatca aagatgaagc actgacgcaa ggtgtgaaat
     8101 ttgtagggat tgacaaagtg atccaatatc caattcgctt gcaaactatc caatcaaaaa
     8161 atatttcagt atctacaagc gacggcaaaa agacaacgat tgatatcaaa tatgactaca
     8221 aagttgattc aactaaagca gcaaaaatgt acaaggaatt tgggaatatc acttcggaag
     8281 atatcgaaag tggatggtta aaatctaagc ttcaaaaggt cgctcgtgaa gtttatgcga
     8341 aatatagtct gcttgatgtc ctttcaggag attcctctaa agttgaagct gaggtattaa
     8401 cgaactttgc taaatcagtt gaatctaaag ggtttgaagt cgaagacgta acacttggtg
     8461 ttccagatgt cgataaagaa acacaaaaat caatcgatgc gatcattcga gctggtcaag
     8521 aaaatgaaaa agcgaagcta ga
//
LOCUS       DACEXQ010000110         8536 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.110, whole genome shotgun sequence.
ACCESSION   DACEXQ010000110 DACEXQ010000000
VERSION     DACEXQ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.110"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(125..1234)
                     /gene="rpoD"
                     /locus_tag="IXH04_001733"
     CDS             complement(125..1234)
                     /gene="rpoD"
                     /locus_tag="IXH04_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAQ1852336.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(1258..3099)
                     /locus_tag="IXH04_001734"
     CDS             complement(1258..3099)
                     /locus_tag="IXH04_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAQ1852337.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(3281..5674)
                     /locus_tag="IXH04_001735"
     CDS             complement(3281..5674)
                     /locus_tag="IXH04_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ1852338.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(5781..6641)
                     /locus_tag="IXH04_001736"
     CDS             complement(5781..6641)
                     /locus_tag="IXH04_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAQ1852339.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(6700..7221)
                     /locus_tag="IXH04_001737"
     CDS             complement(6700..7221)
                     /locus_tag="IXH04_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAQ1852340.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            7541..8260
                     /locus_tag="IXH04_001738"
     CDS             7541..8260
                     /locus_tag="IXH04_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1852341.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            8286..>8536
                     /locus_tag="IXH04_001739"
     CDS             8286..>8536
                     /locus_tag="IXH04_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852342.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQ"
BASE COUNT         2457 a         1872 c         1441 g         2766 t
ORIGIN      
        1 aaaataatga atgaatttcc ttcctataga aaaaaaagct tgagactgcg tagtctcaag
       61 ctttttttct tttaaaataa accaaacagt tttactgatt tctcttctgg tcaatagtcc
      121 acgcttattc taagaaatct tttaattgtt ttgaacgaga aggatggcgc aattttctta
      181 atgctttagc ctcgatctga cgaatacgtt cccgagtaac accaaatact ttccctactt
      241 cttccaatgt tctcgtccgg ccatcatcta aaccaaaacg taagcgaagg acattctctt
      301 cccgatcagt caatgtatct agtacatctt ccaattgttc ttttagtaac tcataagctg
      361 cgtgttctgc tggacttgtt gcttcctggt cttctatgaa atcacctaag tgagagtcat
      421 cttcttcacc gattggcgtt tcaagggaaa caggttcttg cgcgattttc aagatttctc
      481 ggactttttc tgttggaaga tccatttccg caccgatttc ttctggcgta ggttctcttc
      541 caaggtcttg cagcaattga cgttggatac gtatcaattt attaattgtt tcaaccatat
      601 gaactggaat acggatcgta cgtgcttggt cagcgattgc tcgagtgatg gcttgacgaa
      661 tccaccatgt agcataggtt gaaaacttaa atcctttgcg gtagtcaaat ttttcaactg
      721 ccttcatcag tcccatgttt ccttcttgga tcaagtcaag gaactgcatg cctcgtccta
      781 cataacgttt agcgatactt actactaaac gaaggtttgc ttctgccaga cgctgtttcg
      841 cttcttggtc gccttcttcg attttcaatg ctaacgctac ttcttcttcc gcagttaaaa
      901 gggagacacg gccaatttct ttcagataca tacgaaccgg gtcattgatt tttacaccgg
      961 ttggcgcaga aagatcttct gtttttgctt gttcagcttt tttctcagca ctcttcaagc
     1021 tgtgaattga tggatcacca ttttcatcca ccacactgat acctgcgtct tcaatctttt
     1081 ggatcaattt gtccatttca tctgcattca aagtgaatgg tgtagcaagc tgattggata
     1141 aatcatcata tacaacttgt cgtttgggtt tgttttcttt gatgaaagct gctactgctg
     1201 cttcatacgt ttttttatct gttccatttg ccataaaaga aggcccccct tgagtagcta
     1261 tttggcttgt tttaactgtt tcgttaaatt gatgatttca atcgtcaatt caagctcaag
     1321 ctgtttgttt ccagttcgtc tggcttcttg ctgcatttgt ttttttaatg ttatttcatc
     1381 agctaagcca gatttgctga tcacatctag caagtcttgg atttctcgtg gcgaactttc
     1441 ttctgccacc ggtaaatatt cgatttccac caccaaccgc cttaaatttt catcttgcaa
     1501 aaagtctaaa aactctgcaa ggttgaattc ataatgttgt gagacatatg catcgaataa
     1561 aaaataaact tctgcatatg catcatgaat aaactgaaat tctcgttctt ttaaaatatt
     1621 acggacagaa acttctttaa atgcacgata taatagaatc tgctctgcct tttctacttg
     1681 cgtcaaaggt ctctttttcc gtgcagtttc ttgtctcgta acctgagtgt gttgactttc
     1741 ctgaggttct tgacgatttt gctgatttgt ccttcgaaga tcgcgcagtt gtttttgtat
     1801 cgtctctctg ccgatctgaa actctgttga cagttgagat agatacatat cttgttcgat
     1861 tggtgaaaca actcggctaa gttcaactag taattcttcg aggtattcga tcttgtcttt
     1921 ttcattatct aaatttttcc cttgttttaa ataacgtgat ttaaaagtaa agactgtttc
     1981 gcgcccatga gccactaatt cttggaaaga tgcttctcca tacttacgga gatattcatc
     2041 aggatcaagc ttttctggaa cgactacgac agaaagtgct aatcgactgt tttgccgaag
     2101 caattcaatc cctcgattgg ttgcttcaaa acctgcgttg tctccgtcat agcaaaagac
     2161 caattctttc gtcagctttt ccatttgttg gatctgttga gctgttagac ttgtccccat
     2221 agaagctaat ccagttttga taccagcctg ccatgcagca atcacatcca taaacccttc
     2281 gaacaacaaa atttctccgt ttttccggat atcgcttctt gctttatcaa gattaaataa
     2341 aacttgtctt ttattgaaca gttctgtttc tggactattc aagtatttgg gctgatcttt
     2401 ttcctgccct ttttcaggtg caagccagcg tcctgaaaaa ccgatcgtct ggccttgaga
     2461 attacgaagt ggaaacatca ctctttgata aaaacgatca gacatacggc catcatcatg
     2521 cgtgacaaac aatccagagc gttctaacaa ttcatttgaa attgattcat tcttgaatac
     2581 ttgctgaaga aaagaacgct cattaggtgc aaaacctatg ttgaattctt caattaattc
     2641 taaagacaac ccacgacttt gcaaatattc taaagctggc tgtccagctt ttgtatgcaa
     2701 tagcatgtga tgatagattt ccgctgcttt ttcatgagca gcaatcagtt tccccgtagc
     2761 agattcagga ttttggacag ctatagtacg ctgacgccat tgatcttcta acggtatctg
     2821 ttcgaagtct gccactttta ccactgcttc tggaaaactt agaccttcta gttcttgaag
     2881 aaaagtaaac acgtttccgc ctttaccaca accaaagcaa tgaaaaatct gcttgtcttc
     2941 tgcaacagaa aaagacggtg tacgttcttc atgaaaagga cacagcccca agtaattttt
     3001 accagacttt ttcaactgga catattgtcc aatcacctca acgatattcg tttgactgcg
     3061 tactgtttcg ataacctctt gaggaatccg ttgagccatt ttttcacccc ctgctctatt
     3121 gatatctttt gggtttaaca gaagatattt caggagaata accggtaaaa aatcgcacta
     3181 actgaaatca gtgcgatttt aaaggacaca attatacatg tttgattgta tcatatttca
     3241 ttgctttttc aagtataaca catttctatc tcattaagca tcaatttgct actcttttca
     3301 gtaactgatt ttgaatatca gccttccagt acaatactcg aagaacaata tttacgagca
     3361 tgaatacaaa gaagtatcct acgctctctc ccaaccctaa gtttacttcc aaaattccat
     3421 ggaacagcat tgcagctaca tatatcccta cgatcgtcgt tactgctagg aagatgcgca
     3481 atgggttgaa gggataacaa gcttttacaa ctgccatgca actgatccca atcagcaagt
     3541 aatacatcaa agtagtagtt tcagcatttc cccatgcgaa tgtttttcca agtagataca
     3601 ccgcaatgat attcactact acaagcaagg catttggtaa agcattaata agtgctgtag
     3661 gcaaataacg gtaagtgatt tttcttttat cttgttcaaa agataaaaag aaggaaggat
     3721 acccttcaat cgctaaatca atcagcgtga tttggatagg gataaacgga aatccaattg
     3781 ctgtaacggc acatataatc gataaaataa atgaataaat cgttttgata aaaaagatgc
     3841 ctgagacttt cgtcacattg ttgaccactc ggcgtccttc aaacaggact tctggcaatg
     3901 tagtaaaatc agagtccaac aataccagat tcgcaatctg acgtgttgct ccatcacctt
     3961 ctgccatagc aatgctgcaa tctgcttctc ttaacgccaa aacatcattc acaccgtcgc
     4021 ctgtcattgc aaccgttctg ccgctttctt tcaattcatt taccagcaat ttcttttgct
     4081 gcggactcac tcgtccaaaa accgtatagc gatgagcagc ttctcgcact tccatttctt
     4141 cagtaatttg agaaagatcg acatacgcat cataattcgg taatcctgca cgtctagcga
     4201 tattggagac agtaaaagga ttgtctccag aaatcacttt cagatccaca ccttcctcat
     4261 gaagataagc tagtgtttct tgagcgtttt ttcggattgg atcatcgatt tcaaacagag
     4321 caaccggctc aacaaatgct ggtttttctt cgttcaattg ctgatttgga gcgatcccca
     4381 acataagtac gcgatagcca ttcgcttggg ctgtttttag actgtccgga agccgatctt
     4441 cttccaccaa acgttcaggc gctcctacgt acacgactcc cagttctgga aattccatcg
     4501 cgccccattt gcgatcagaa gaaaatgcga ctttgtttgt cacatcatac cgattagaaa
     4561 gctcaaaata atctctcata gcccgcatag tgatgttgtt atcactgctt tctgccagat
     4621 acgtccccat aatttctggg atggctttct cgtacagcgg atgtagtgct tctacccgct
     4681 ggatactcat tttcccttca gtaatggtcc ctgttttatc caagcataat gtatcgacat
     4741 gggccagcgt ttcaattgaa tacatgtctt gaacaaggat tcgttttttt gccagtttgg
     4801 ttacccctgt cgttaatgca atacttatta gcaataccaa accctttgga agcatgccta
     4861 acaaagctgc tgcagatgca ataacagaaa ttttgataga agcatcgcgg atgaacagtg
     4921 cttccaaaaa tagtatgatc cccaaaggaa taatcacgaa actagtgaat ttggatactt
     4981 tgcgaatcga atctacaagt tcagaacgaa tagctttgtg agtctttgcc tcgctagtca
     5041 agcggacagc ataattatct gcacctactc ggataacttc agccatgaca gaaccgcttg
     5101 tcaaaaagct tccagaaagc aattcagctc cagtttcttt gacaatcaaa tcagattcgc
     5161 ctgtcaaaag agcttcattt gcttctgccc gtccagatat aacctgtaaa tcggctggta
     5221 tctgttctcc tgctccgatt tttaccaaat ctcctaatac cagctcttta gtagaaatcg
     5281 attgttcttt accgtcccgg atcactagta cttctgattg ggaaacaatc gataatttcc
     5341 gtacaagatt tcttgcatgt atttcttgat aaattccaat aacgatattg acgacaataa
     5401 ttgctaaata cgccatgttg gaatacgcac ctacaaataa cagacaaatc ccaataccaa
     5461 agttcagaaa attgaataac gtcaatatat tatcgctgaa tatttgtttt gtactttttg
     5521 atgcatcttc ctggtagtca ttttgctggc cagaagccat tttctgaact acctcttgct
     5581 gtgtaagtcc attcattttc tttttctcca ttttccgcca actttcaaaa ataatcacgt
     5641 gtttaagtct agcgtgttcc aagattaaaa acaacttgtt tttataaaat ttatagaaaa
     5701 acacacgaat gattttacaa aaaagatgcc aacctttttg tttgaatcac ccgtgtgctg
     5761 tttttattat caatctatct ttacttatat tgttcctcca aacggctcat ccaccaacct
     5821 agtgctgtac cggctataaa aagaacagga caagctagta ccccagcaac tcccaatccc
     5881 gcgtaatcgg taggaatgat cataatcgtt gaagcaaaaa ctacacctaa aatgaaatgg
     5941 aatagcttgc tatatgcctt tgagaaaatg taatccatga tttttgacaa tccaagaaca
     6001 caaagaatcc caccaattcc gatcggcaca atcacttcaa gtcgtacttc tttaaatccg
     6061 tcagccatcg ctttatacaa tcccatatac accaaaaaat tagaagggct caatcctggg
     6121 acgatcatac ccaaaccaat caatccgccg gcaatcagcc atgttccaaa attctgatcg
     6181 acttgcccgt caaataattg agcgccgtat aataaaaaca aataagccag aacaaaagta
     6241 acaaccatga tgatcacatc agaagtatct cttccttttt tccctgcttc tttccataac
     6301 gcaggaacag ttccagcgat acatccgaca aaaaaccata atatcgttgt ttcatacgca
     6361 cctaataaaa aactgactaa aaaagaaagt agaaagatgc ctgtcactcc gcctagacca
     6421 acaggcagaa aaaacaaaac attttctttg aaattttttg taaggtgggc taaaaaagaa
     6481 atcagccgct catacatgcc aaagacagct gctaatgcac caccgctgac tcctggcaag
     6541 ataaatcctg aaccgataaa cattcccttc acaaaccgta acatccagtc tttggttgtg
     6601 taccccactt cttgattttc cattttttgc cttttttcca ctatttgcgc actccttctt
     6661 tcatttaatt ttattcatga ttgaacattt cctgctattt tacttaaaga ctttttcata
     6721 ggtctctatt attttttcat tcattttgac aatatcagga taaaacatgt tacctccatt
     6781 atgatagaga tagccgccat tgtaaagcaa ggactggatc cgccaatatc ggtattgttt
     6841 cccttcctca tttcctaaaa gtgggctcag cacatcttta gaatacgctt ctgctaattc
     6901 tactgtgttt tctccgccat gattttttac ataggcaata taatctttcc caaaattata
     6961 tgcttggaca gctgtccata aatcgcatcc ttgtttcttt gcttctacta tcatttccgc
     7021 caaatatttg acaccttgat caatactgtc agctggatta tcaatgatct cagcttctcc
     7081 atgcagactt tccgagctct gcatgagatc tgtccctttt ccttttgatt cggtcaatat
     7141 tatcgccttg atcaaattct cgtagttttc catatgataa ttttttgcag ctgttgttat
     7201 ttctatttga tagctttcca ctcgttttac cgaacgataa gttcttcctc ataaaaacaa
     7261 caccgcacca ataaccgtca gaatgatcat gtaaaaaaga atacggataa tagataatcg
     7321 ttttcgcttc atttcatcac ctagttattt tgacatcttt ttttatctta ctatttcttt
     7381 atcttcttgg ctatttcttc cttgatttct gtgtacgcct taagaaaaaa tgaattttcc
     7441 tgtatttttt gacatcttac gaaaatgatc gttgtttttc agtaaaaaca attgcccaat
     7501 gactgatata atcgttatac tttacacagt aggtgattat gtgaaaaaag aaaagaaata
     7561 tgcaaaaatg ccagatggga gcgaaattta ctatgaaaag agcggtcaag gcttccctct
     7621 ctttttactg catggcaatg acggcagcgg ccgctttttt tcagaacagg tccctgtact
     7681 cgaacgctac tataccgtgt atctagtaga tagccgagga catggtcgtt cgacaaacga
     7741 agcatctatg ttgaattttc agttaatggc cgaggatctg aatacgatca tgttgctgga
     7801 aaaaatcgat caggcagatt ttcttggatt tagtgatgga gcaaatctag cactggtatt
     7861 tgccagcagc ttccctaaaa aagttcatcg attgatttta aattctggaa acacgcttgt
     7921 taaaggcgtt cgtttttctg ccagagtcat cagtaatatt cattatgcat gggtatggtt
     7981 actctcttta tttcgcccaa gtcttcgcaa aaatctttta gttatcaaac ttttgctgca
     8041 tgacatcggg cttacagaaa atgatttaaa aaaaatcaac tcccccactt tgatcatcgt
     8101 gggaaaaaaa gatgtaatca agctaaaaca ttctttgtat atagctaaaa ctataccaaa
     8161 agcttccttt gtgcttgtga aagaacaagg acatgaatta gctagaaaag atcctgaacg
     8221 cttcaatcga gaagttcttc aatttctttc tgaaacataa ctattaggaa tagaaggtga
     8281 tttatttgtt aaaaaatacc atacaatggc tgaaaaacca cctaggactg ttcaaaacaa
     8341 tctttttgat ttctgtcatt gtcattattg tcggtgaatt gatgtccatc ggtaaaactc
     8401 tatctatcca gcaattagga gaaacgtttg ccactatacc tgtatggaaa agcggactaa
     8461 tgctattgat tggtcttatc tctgtgttac ctatggttgg ttatgatatt attttgaatc
     8521 ggatgcttga acaaaa
//
LOCUS       DACEXQ010000111         8366 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.111, whole genome shotgun sequence.
ACCESSION   DACEXQ010000111 DACEXQ010000000
VERSION     DACEXQ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.111"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(312..842)
                     /locus_tag="IXH04_001740"
     CDS             complement(312..842)
                     /locus_tag="IXH04_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1852343.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
     gene            complement(1024..1680)
                     /locus_tag="IXH04_001741"
     CDS             complement(1024..1680)
                     /locus_tag="IXH04_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAQ1852344.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            complement(1807..2280)
                     /gene="dfrF"
                     /locus_tag="IXH04_001742"
     CDS             complement(1807..2280)
                     /gene="dfrF"
                     /locus_tag="IXH04_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HAQ1852345.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            complement(2699..2917)
                     /locus_tag="IXH04_001743"
                     /pseudo
     CDS             complement(2699..2917)
                     /locus_tag="IXH04_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2948..4162)
                     /locus_tag="IXH04_001744"
     CDS             complement(2948..4162)
                     /locus_tag="IXH04_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAQ1852346.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(4503..4745)
                     /locus_tag="IXH04_001745"
     CDS             complement(4503..4745)
                     /locus_tag="IXH04_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ1852347.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(4777..5334)
                     /locus_tag="IXH04_001746"
     CDS             complement(4777..5334)
                     /locus_tag="IXH04_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ1852348.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(5347..5535)
                     /locus_tag="IXH04_001747"
     CDS             complement(5347..5535)
                     /locus_tag="IXH04_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ1852349.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(5548..6114)
                     /gene="amaP"
                     /locus_tag="IXH04_001748"
     CDS             complement(5548..6114)
                     /gene="amaP"
                     /locus_tag="IXH04_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ1852350.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            7344..7817
                     /locus_tag="IXH04_001749"
     CDS             7344..7817
                     /locus_tag="IXH04_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAQ1852351.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            7826..8053
                     /locus_tag="IXH04_001750"
     CDS             7826..8053
                     /locus_tag="IXH04_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1852352.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT         2545 a         1654 c         1248 g         2919 t
ORIGIN      
        1 ttttttgaaa tcaactgtct ttctcatatt tactattcaa ttttcatagt gatataggta
       61 caatttatat cactactgta aaaatggggt tttgccttat aaatacagcg ttttccgctt
      121 gttagtgtat agtgtttctt gttttttgtg ccccccttgt tagataacgg gggctttgaa
      181 cggctcgcaa gctcgccgaa ccgccgaagc gaaccgcccc cttgcaagca tggcggaaaa
      241 atcttaagga ttttcccgcc aacgtggtcg gatcgctccg tcggtttaac agcttttctg
      301 tttctctcta gttatctacg catataaatc aaatcggtta ttccgttata agtctgtgtt
      361 ttacaaagcc ttaactttat ttccctgtct atgttgccca aaagacgaac cccatttcct
      421 aacagagtag gaataacaga aatgtaatat ttatcaatca tatttttgct taccaactgc
      481 tgaacaagat ttgctccgcc acaaatccaa atatcctttc ctgcttcttg ttttagccgt
      541 tccaataata aaacaggatt ttcacttgta aaatgaattt gttgggaaga attgcactcc
      601 ttatgtgtta atacataagt tgtaaattta ttatataccc attcttgcgg agaaagttca
      661 gtaacaacct gatgagaagt attccacccc attaagacag tatctatatc tttggtaaat
      721 tctgtataag tgtcgatgtt ttcattgtca tttccatgac catttagcca agctactcca
      781 ccattaccgt ccgcaatata tccgtcaagg ctcatggcaa taaataaaac tacttttctc
      841 attttttctc ttttataaat acaaacagga atttatttct cttttttact cataagggaa
      901 caactgattg ctacaaataa acccattaaa atccatggaa atgctgctct tataaaatca
      961 ctaaccatac tttattcctc cttaaattcc aatttcctta gttctacaaa ctgaaatttt
     1021 tgcctatgaa tgtatcttgt tctttatacg caaacaggct tctgtgtgca taatccaatg
     1081 tcttgaaaac ggcatttgaa ttagtccaca aatatcttta ttacaccaat aatcaatcag
     1141 ccaaattgct ttttcattgt cgtttactac attcaacgat tctaagtatc cttttctttc
     1201 tttcggtatt tttcttttca attcatcata agataaccgt tcaagcattt tctcagtgct
     1261 ttcccatact tcgaaaatat ataaatataa ttcttccaga ttaagctgtt ttgaaaagtc
     1321 tgcaatctgc tgtttcatga gttcatttcc tgttgtaatg ataggtgaat tgatacgctc
     1381 ttgataattg cctgcaaaaa atacttgttc atcttcattt atcactgtat gcacaactat
     1441 atcttcaatg cggaaaatat gccaaataga ataagcaatc gtcttactat gataaccatc
     1501 agcgtttatg aaggggattg agttaaaatc ttctctgcat aattcttctt tgaaagatac
     1561 taaggtttgt attaactgac ttcgtaaagt aagtaaagta tcaatacccc tcttataagt
     1621 atctttcttt tttatttgtg cctgcattgt tttattaagt tcagaccatt ccttattcat
     1681 attcaatcct ctctcaaatt ctgattttat cgccccatac aaactggaat ttgtcttaca
     1741 acaatttata tatgcataaa aagcaatata tttatgaaaa caccctaaat ccaaaattta
     1801 ctcaattcat tttttcctta cataaaatgt tctcgtatat ttcacttcct caccaagtgt
     1861 ttcccctatc aaaacttcaa aatcattgat atcaaattct ggaaagaatg tatctccatc
     1921 ttcaatattt aaatctactt ctgtgatata cattttatct actatttgca aagcttcctt
     1981 aaatagtcca tatccaccag atatgtatac atctcgtcct ttagccaata ataatgcatc
     2041 ttctaatgat ttaactgaaa ctaaattatc tccttgatac tctgttgtgg tggaaacaac
     2101 aatattcatt ctattaggca acggatgacc gatttcttca taagactttc gccccataat
     2161 aaccacatta cccgttgtta actctctaaa ttgcttttgt tctcccttta ttttccatgg
     2221 tatattacca ttcttgccta taacattatt ctttgacctc gcaacaatca aacctatcat
     2281 ctatttcatc ctccataata tgatgtcgta cctgtgattc catcaagtgc accacatctt
     2341 aacaaactca aaattatctc gaactaacca gctcgacaaa ttcctttttt gtcttcatct
     2401 gcattatatc atattgttat aagcattgcc atttcttttc tgcttttgaa aagttactta
     2461 tcagcctatg ttttgtaaag ggtgatattc agcatttctc acgcaacaag cccttgacaa
     2521 accatagact aataagtttt aaggtaatca agcagaaatg aaatagtctg ctgtttcacg
     2581 gcaacaaaaa agagatacca acacacttat gtagtatctc ttagtctagt agcaatattc
     2641 ttttgtttgc aagcaggtaa acatgggctg tttcttaata tctttttgtt tgggaaacac
     2701 ctttttccca tcctttcacc gttgccatct ctgctgcgca caatgccaca gggataaacc
     2761 atagcaaacc gcctaataac aagaaaaata ctaagctgaa gcctgaggtc gcgaatgtgg
     2821 gatattcata taatgaaacg accattgaga tggtcatagc caaaaagcca aagaacgtta
     2881 atttttttgc aggaattttc gttttgcttt gttgcatgaa tcttcctctt ttctatttga
     2941 atttgtttca attgagtttg ttgccaaaga agaaaaagtc caatccttct tcttgttctt
     3001 cttttgtagt agtgatcgtt gtaaagtgca tcgccgattt taaaatcact gttgtcagca
     3061 cataggcata cacggcagta atgatgacac caatcacttg aatccctaag aagtaccagc
     3121 tgcttggatt cgttagtaaa tggctgttag atgcaaagat ccctatcaga atcgtacctg
     3181 tgaatccgcc catgccatga acaccccaca catcaagtgc atcatcccat tgtttctttt
     3241 tacgaaaatc aacagcagca tgacagacga tacccgcaat gatcccaatc agcatggcac
     3301 tttttgcttc tacataaccg gcacaaggcg taatggctgc caatccagca acggatcctg
     3361 ttagtacgtc gacaaaatca aagccatttc cttttacttt tgcaatgatc agccaagtaa
     3421 tcatcgcaaa tgcaagagcg atgaatgttg aaccaaatgc attgactgct tgttcatctg
     3481 ctcgaagtgc accaccggaa ttaaaaccga accagccaaa ccaaagcagt cctgtaccga
     3541 tagcagctgc cataagactg ctatgctgac ttctttctcc taaaggaatc tttctttgac
     3601 caagtgtgag gatacatgct aaagagccga atccagctgt cgtgtggatg actgctccac
     3661 cagcgaaatc tctaaatccc atctgatcca aaaatccgcc accccatatc cagtggcaaa
     3721 cagggaaata aatcagcaga ttccaaaaaa taacaagtat aatatagcct tttaaattca
     3781 atctccctgc aaaagctcct gtcatcaaag gaacggtaat cacgataaac atcagttggt
     3841 aaagaaagaa cattaaaaat ggaatcgttg caccatgaaa catgttgggg aagcttgtaa
     3901 catgtctcat caaaaagaaa tctgccgggt ttccaataac accgccgata tcccgtccaa
     3961 aggccaatcc gaatccacca aatacccaca taatcgttgt tatcccaacg gagagaaaag
     4021 attgagacat gatcgttagt atattattct tccgtgctaa tccgccgtaa aaaaaagcta
     4081 gaccaggagt catcaggcaa accattgctg tacataaaat catgaatgct gtatctcctg
     4141 tattgatagg taatgtattc atttttttcg cctcctttct acgattacga gtataaaaga
     4201 aggtgtagtt gtttttttct tctgtttttt tgaattaata cttgcattta taaaagacag
     4261 acaattaata aaagcttgat gtgttgtgct ctttttcgtt tttcacacaa ataaagacaa
     4321 aaaataaacg gtcttctttt tatagaaaac cgtttatttt cgtctttttt tatttttaaa
     4381 gatatttttt cataaggaat aaagaatact tcatataaaa tatataaaaa agaaccgcag
     4441 cgagtgtcgc tacggttctc aaatttgttc ttcatttcaa cataagtttt tcttcaattt
     4501 gcttataatt tacgtgcaaa gaatgaaacg actaatacta aaatgattgc accgatcaat
     4561 gatggaatga tagccattcc tgcaattgtt ggtccccaag ttcctagtaa agcttgtcca
     4621 attgctgaac ctactaaacc agcaatgatg tttgcgatga tccccattga tgatccttta
     4681 ttcgtgattg ctcctgcaat tgctccaatg ataccgccga cgattaatgc ccaaataaat
     4741 cccattttaa attcctccta actgacttct tacttcttat tgtacacgtg gttcgttatt
     4801 ttctttgact ttttgtgcac ctttgcctac agcagatttt gctttatctg tttggcgtga
     4861 agcaaattgt cctgtggatt ttgctgcttc tgttactttg tcttgtactg tttcttgatc
     4921 tttttggtat tcttcttgtg ttttgatgtc tgcaacattc acattcactt cgataacatc
     4981 taaatgtgtc atattgttta cttcagtact gatcaattct ttgatttggt tgtaaatatc
     5041 ttcgatgtct ttaccgtatt ctacgatcac atctagatcc acagctactt gtttcttacc
     5101 tacttctgta ttgatacctg ctgttacgtt atctgtgttg actaattttt cagccatatt
     5161 tgagaaaaat ccgccatcta atgtcaatag accatctaca tctttcaatg cataaccgat
     5221 gattttttgg atgactttgt cttcataagt caattcacca ttcatcatgc ggttttctcc
     5281 tttttcattt gccaaaactg atccatttgt tgtattcatg tttttgtttt ccatctttgt
     5341 ttcctcctat gatttaaaaa attgatcaac tattcctgac tgtttcaaat aaaaaccgat
     5401 tcctgcgcca attgctgtca tgattatgat caacaatgtc ttgaaaaatc caatcgttaa
     5461 ccataaaaca gctagcagta gtccaagacc cccaaagata atagggagct tgtatgctga
     5521 aaatagttct ttcattattt ggccctccta ttccactcga gagtgcttgt agtctcttgt
     5581 atcttcttgt tcttcataac cgctgtatgt gactgccact ttgaccgttt cttttgttcc
     5641 gagtacttta gttacttctt cttcgatatc ttgcatcaat acacctgttt tccctactaa
     5701 tgaagaagtt cgtttcaagt ctccgttgac atggatatgg attcgatttt tcgttgaacg
     5761 tatccgtact ttaggtgaat gaatgaactc ttcttcgtgt aaatgggaac gtaccaaccc
     5821 ttcgatcgct tttttgtcga ttgttagttt gccatcttcc cgtttcataa cgaaagttcc
     5881 tcttgacttt ggatagaaaa gaacgactag caagatgata atagcaagaa cggcaaatac
     5941 tacagctgcc cagaaaatat attgttgcat gtagtaattc gttaaagtaa agaagcgaaa
     6001 tcgttccaat tgttcaggca taatatacgg cgcattgatc aacgcaatcg ctagaaatac
     6061 tgaaagtagc agtaaactca tgataacacc gattattttt aaccccttat tcattttcca
     6121 cctcctataa acttattttt accctgtaaa taactctacc gacaatcaca gtatagatag
     6181 cgaacagtta gcatagcatc agtaaataaa gcgctaccaa acattgctgg atcataacgg
     6241 cgttttattg ccagtctgat actgaaataa caattgctta cttattctct atttactttt
     6301 gattgctggc tatacttaaa ggatattatt caagattaaa aattgcaaag attatgctca
     6361 gttattatta caagattcta tttatttatt aaattttttc ggagaattaa aaaatacaat
     6421 aaataacttt agttacttta atgcgttctt actttatttt tatattatca tttttcttat
     6481 tcacttcttt ggtttatact attcttataa ataaaaacga ggtatgtcct atgatgaaaa
     6541 gattgtacta ttcattgatc ataacgattg gctatttgat tgtatcaaat ctaggaaata
     6601 tggtatttgg tatcagtaaa gaattcagtt ggactactac attatgggaa tctctattct
     6661 ttttcatctt cgtatttttg ttacaaaatt atcgaaaaaa ataaacatgt gccatctatc
     6721 tgttggcaca tgttttagga tttatggtac tttttattcg gcaatccatt ttatttttct
     6781 acttcttctt aatacctcta acacttttga tttataaact aatccaatgc atctacttgc
     6841 gcttattaag ctatttcagc tagatagtac caccccatgc gtcaatgctt gattttcctt
     6901 ttgtataaca ttgtataagc aataaaggac aaataagtgg tgattacacc aaaccattga
     6961 ataagaaaaa ttctgatacg taaataaaca tgaacttgaa aaaattaatg aatcggttag
     7021 ctactacgta gttgtctttt ttctcataca gttctgttaa cttacacttc tcttcgctag
     7081 ataataaaaa atcctccaac tgtattgtcc aatataatcc caggcatcta aactagcatt
     7141 atagaagaga cacacataga atctaaaata actggtttta aatgtttctt acaatcaaaa
     7201 aaagctgtta aatgcactcc acaccttcat actctatcat tttttataaa cgatatattt
     7261 ttattgtttt tcgataaaaa atagttgttt tacaataatt attctgctat attttagcta
     7321 tcaaagataa aagagggata aaaatgaaat ttaaaacatt gctattaaaa tttgtcgttt
     7381 taggtataag tgccgtattt attttgttcg gcgtactttt ttatactcag atcgctacaa
     7441 gcgaaaaagg attcaatttt gattggcaga ccacattatt tgtttctgtc atttactttg
     7501 cccttctcct agggatcgtt attgcttatt tcttattgaa actattgaaa aaaatcgaag
     7561 ataaccaagc tttttccact caaagcttaa acatactaaa aaaaatcgaa cgaaccattt
     7621 tgtttattgg tttcgccttt tttggcttac ttcctaaatt tatgaaagta gcggatttag
     7681 acgatgctcc aggtttgatg ttggtaggaa tcgctgtcgt attcattcct tttgcagtat
     7741 acacattaac aagcgtgctg gaaaaactgc ttgaacatgc gattttactt aagacagatc
     7801 atgatttgac agtgtgaggt gcgacatggc aattatcgtt aatttagatg tagtattagc
     7861 tcaaaaaaaa atgagtgtga ctgaattgag tgagaaagtg ggcattacga tggcaaacat
     7921 ctctattcta aaaaatggaa aagcaaaagc catccgtttt tccactctcg aaaaaatctg
     7981 ccaagttttg gagtgtcaac caggtgattt gttgacctat aaaaatgact cgaatactat
     8041 accagaagaa taaaataaag tagaacaaac agtaaataaa aaataatctg aatgatagat
     8101 gtgtgagact tttagaagtt atttacgtct attattcaga ttttttattt cgattgaata
     8161 ccgttttatc tataaataag atatgtactg actcatacaa agggggcctt ttcttctcca
     8221 tgtactatca cttttcctga tacaactggt ttcctgttac gttgtagttg gatctgcttt
     8281 tagtataata ctttattcat tccaccagac tgatagcagt ttgagcagtc taaataatca
     8341 tgtagtatta caggccattc tgtttc
//
LOCUS       DACEXQ010000112         8327 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.112, whole genome shotgun sequence.
ACCESSION   DACEXQ010000112 DACEXQ010000000
VERSION     DACEXQ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8327
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.112"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..875)
                     /locus_tag="IXH04_001751"
     CDS             complement(<1..875)
                     /locus_tag="IXH04_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HAQ1852353.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNIL"
     gene            1030..2589
                     /locus_tag="IXH04_001752"
     CDS             1030..2589
                     /locus_tag="IXH04_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAQ1852354.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(2700..4631)
                     /locus_tag="IXH04_001753"
     CDS             complement(2700..4631)
                     /locus_tag="IXH04_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ1852355.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(4649..5563)
                     /gene="pfkB"
                     /locus_tag="IXH04_001754"
     CDS             complement(4649..5563)
                     /gene="pfkB"
                     /locus_tag="IXH04_001754"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAQ1852356.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(5560..6312)
                     /locus_tag="IXH04_001755"
     CDS             complement(5560..6312)
                     /locus_tag="IXH04_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ1852357.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(6472..6681)
                     /locus_tag="IXH04_001756"
     CDS             complement(6472..6681)
                     /locus_tag="IXH04_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852358.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(6692..8020)
                     /locus_tag="IXH04_001757"
     CDS             complement(6692..8020)
                     /locus_tag="IXH04_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852359.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
BASE COUNT         2352 a         1712 c         1517 g         2746 t
ORIGIN      
        1 aggatgtttt tcgtggagcg atagttttgt tctagcaaaa ttactgcagc atctggataa
       61 tctttttcaa aatccaagat gttctgcata tctgcaccgc gccagccgta aatactttga
      121 tctgcatctc ccacgacgca caggttccta aatctacctg ccaataaatt gactagcgta
      181 tactgtgcgt gatttgtatc ttggtactca tctacatgaa tataatggaa tttgttttga
      241 taatacgtga gtgaatcagg atgttcttca aataatcgaa tcgtattcat gatcaggtcg
      301 tcaaagtcca tacattgatt atattgaagc tctttttggt aagcagcata acattttgcc
      361 gcaatctctt cgtaataact gccttgcatt tttgcaaaat cctgaggggt ctgcaggctg
      421 ttttttgctt gagagattgt tcctaatata cttcgaggat catatttttt cggatcaatg
      481 ttcagttctt ttagtatccg tttcatcaag gtcagctgtt cagaagaatc gatgattgta
      541 aagttccggt tatacccgat aaaatcaaca tctcttctta aaatacgcac acacatcgag
      601 tgaaaagtcg atacccagac atcttcgcct ccagatgcga gaatcgcatt gacacgttct
      661 ttcatttctt ttgcagcctt attggtaaaa gtaatcgcta ggatgttcca aggattgact
      721 tctttttcct caatcaaata ggcaatgcgg tgtgtcagca ctctcgtctt tccacttcct
      781 gcacctgcca ttagcagtaa tggtccgtca gtgtgaagaa cagcttcttt ctgacggggg
      841 ttcatcccgt ttaataattc agctttggat gacattaaca acatcctttc ttttatcaat
      901 cttgttaatt atagcatttt cttggctttc attaaactag ctagttcaaa atcatttgtt
      961 tctttttatg gatttttcct tgtcaattcg ttgatttcgc gctaaattaa aagaaagcga
     1021 ggcgtttgaa tggaaaaaaa aggaaaaatc aagaaattag ataagactca gatatatttt
     1081 attctaaaag gaatatttgt tggactgctg acaggaatcg tcgttagcct gtttcgatta
     1141 tctatcgaaa agctaagcga tttagttcga agtatctatg aaatgagtcg ggaacagccg
     1201 atttacttaa ttggcctagc tatttgctgc atccttgcag catttttcgt gggttatctg
     1261 gtcaaaaatg aaccggatat caagggaagc gggattccac aagtagaggg acagcttcgc
     1321 ggggaattat caatgaactg gttctctgtt ttatggaaaa agttcattgg cggtgtactt
     1381 tcagttggtg cgggcctttt tctaggtaga gaaggacctt ctatacagct tggcgcatcc
     1441 attggacaag gagctggaca attgtttcgg agtccatctt cagaagaaaa gattttaatt
     1501 tctagcggtg caagtagcgg acttgcagca gcattcaatg ctcctatcgc aggattactc
     1561 tttgttttag aagaaatcca tcactccttt tctccacttg tttggctgac atcatttgct
     1621 tcagcaatca ctgctaactt cgtctctctt tactttttcg gcttgagacc tgttttatat
     1681 ctaggtgacc ttccttcttt gccgttgaaa tattatggtt ccttagtgat cttaggagta
     1741 cttctaggca tcttaggttt tatttatcag aatgtcttgc tggctttgcc aaaattttac
     1801 aaccgattgc ctttaccagc ttattttcac gggttcgttc ctttcttgct gattttgcca
     1861 attggttatt tatggcctag tactctaggc ggaggcaatc aaatcgtttt agcttttgga
     1921 catgcttcct tgccaatcat tatgattatc ggtttgtttg ttcttcgatt cgttttttcc
     1981 atgatttctt acggtggggg tcttccaggg gggattttcc ttccgatatt gacattgggg
     2041 gctttaatcg gtagctttta tggaaacatt atggttgatt tcgggatgga tccaatctac
     2101 atcaaggatt ttgtcgttat tgccatggct ggttatttca cagcgattgg aaaagcaccg
     2161 ctgacagcaa ttattttagt gacagaaatg gttggctctc ttaatcatct gatgccttta
     2221 ggattggctg ctcttgttgc ttatatcgtc aatgaccttc tcggcggtaa tccaatttac
     2281 gaatcattat tggaacgtct acttggaaat actcagaccg cctcactaaa aggtgcaagg
     2341 acaacttttg attttccagt aacggcagag agttctttag acggattaat ggtacgagac
     2401 ttcaattggc ctaaaaatat gctgctaatc tctatacgca gaggaaatca ggaaatcttg
     2461 actcacggtg atacgatcat gaatgtagga gatattttgg ttatcttgac tgatgaagca
     2521 aacttgccaa gaatcaagca ggaaatcgag gctaaatctt ctatcacaaa tttgaagcaa
     2581 tcattttaac gaacatggaa aaactatttt acaacaacta ttcatctaca ctacttgatt
     2641 ctatatgatt ctataaaaaa agaagaggct gggaaaaccc agcctcttct tttttatttc
     2701 tatgcttctg ctactgcagg agcttttctc aataaaccat aaatcacacc agatacgact
     2761 gctccgatag caataaacac caaatacatg accgggtgac tcagtaagaa tacaacgaaa
     2821 atcccgccgt gaggagccag taatttgatt ccgaaagctc cgactaatcc gccagttaaa
     2881 gcagaaccaa taataaaact tggaatggca cgaatcgggt cagctgctgc aaatggaatc
     2941 gatccttccg tgacaaacga tagtcccatc acgatgttcg tcaaaccagc atcttgatct
     3001 gttttgctga atttattctt aaacaaacgt gtagcaacga aaattgccaa tggaggtacc
     3061 attcctccag ccataacaga agccatcacg acacttccgc cagttgcaac agtcgtagct
     3121 aaagttgctg taccaaacac ataagcggct ttgttgattg gaccgcctaa atctgccgcc
     3181 atcattccag ctaataatgc accaagtaaa gcagcattcg aaccactcat acttgtcaag
     3241 aaaccgttca atgcatcatt gattgctttc atagggacat tgaccagcaa catcaagaaa
     3301 ccagtaatca gtaagccaaa cactggataa aagaggatcg ttttaattcc atccaatgat
     3361 tttggtaagt tcttgaataa tttacgtaaa aatacgatga cataaccagc taagaatcca
     3421 ccaatcagtg cacctaagaa tcctgcaccg cctgtattag ctaatgcacc agcagcaaat
     3481 ccaacgatca aaccagggcg gtcaccgata cttgaagcaa taaatcctgc taataccggc
     3541 agcatgaaac caaatgcagc ttgtccgatt tggttgaacc agctagcagc ttgattgtaa
     3601 ttccctagat ttgataattg atcatgtggc acgccaataa attggtcgat catgaaagaa
     3661 agcgcaatag cgattcctcc accaataacg aaaggtaaca tgtgtgatac gccgttcatc
     3721 aaatctttgt aaattctttg tccgatagta ccatccgcat ttccttcttc tgaactgctt
     3781 tcttttccat caccatggaa tactggtgca gtaccactaa gggctagatt gatcaactct
     3841 tctggtttac ggataccatc actgacagga cgattgatca gttcttttcc gtcaaagcga
     3901 ttcatctcta cttttttgtc tgctgcaacg attacacctt ctgcacgggc gatgtcttca
     3961 gcagtcaatc ggtttttgat tccttctgaa ccgttcgttt cgactttgat ctccacaccc
     4021 atttctttcg cttttttctt caaagcatct tctgccatgt aagtgtgagc gatccctgtt
     4081 ggacaagctg taacggcaac aacgaatttt ctttctggat gagcagaagc ttctgcagct
     4141 tgtgtttctt gttctgcttt ttcttctgct tctttttgct cttcagctgt ttggaacaag
     4201 tcttggactt cttctggtgt atttgcttct tttaattttc ctacgaaatc tgcatcaatc
     4261 agcaatcgag atagtgctgc taatgcttgc aaatgtgtgt cgtttgcgcc ttctggagct
     4321 gcgatcatga aaaacagata agttggctgt ccgtctaagg cttcataatc tactcctttt
     4381 tggctttttg cgaataaaac tgttgcttct ttaactgcac tgtttttcgc gtgaggcatg
     4441 gcgattccat caccaagacc tgtagaagtt tgtgcttcgc gggctagaat tccttcttta
     4501 tatgtttcga tatcggaaat ccggccggca tcaaacattt tttggaccat ttcatcgatt
     4561 gcgccttttt tatcagtcgc ttgcaaatcc atgatcatcg cttctttgac cattaaatcc
     4621 ttgatattca tgattatcct ccttgaaact aatcaatttt tgtgatttca acttctggca
     4681 gcaactcatc aatcaattcg cgagctgcca agtcatcaga aaaagctgtt gcgcttccgc
     4741 aagcaactcc ccatttaaag gcttcgattg gatctttcgt acggctgtaa gccccaataa
     4801 agccagcaat cattgaatcc cctgcaccta ctgaattttt caatgggcga actaatacat
     4861 tcgttttata agttccttgt tccgtgaaga gcaaagcacc gtctccagcc attgagatca
     4921 gcacattctg tgccccttca gctagtaatt ttttcccata cggaatgatt tcttctacag
     4981 attggaaggt tgtgttgtaa agctcagcta attcgtgatt gtttggtttg atcaacaacg
     5041 gtttcttaac aagtgcattt tttaggtcat cacctgttgt atcaatcacg aaatctgcac
     5101 ctttttctcg aatgatatca atcagttctt gataaaatcc ttttcgcagg ctagctggcg
     5161 cgcttcctga caatacgacg atatctcctt gtcctactgt tcctaatgct tgcttcaact
     5221 catcgatttc ttcatctgaa atagatgggc ctaagccatt gatctcagtt tcagtgtctg
     5281 attttagttt gatattgatt cttgtatctt cttttacggt tgtgaacaaa gactggattt
     5341 gttctttttc aagccacttc gaaataaatt cgccagtgaa ccctcctaaa aaccctagtg
     5401 ctgtagattg agaatcgatc cgtttcaaaa tacgggaaac attgatccct tttcctccag
     5461 gtaacttgaa gtcttccttc attcggttca aatcgcctag ttttaaccca tctacgcgta
     5521 cgatataatc aattgatgga ttaagtgtta ctgtgtaaat cattgtattg cctccttaat
     5581 tgttgtcttt tcacggaatt cattaacgaa ttctaatgga caacgctcgg ttatgatcgt
     5641 ggctgcttcc aaaggtgcaa cagaggtgaa ggacaactgg ttgaatttgg tatgatcaat
     5701 caatacaaat gtctcttcgg aatttttcat ggctaaacgt ttaacagctg cctcttctgg
     5761 atccggagtc gtgaatcctt gttcaagatg ggcaccgttc attcccataa atgctttatt
     5821 aaaatgaaaa ttttttagtt gttctatcgc attagatcct aaaacagcat tagttgccag
     5881 ctttatcgtt ccacctaaaa taatggtagc aagctccaaa tcaactaaat ttgccgcatg
     5941 ttgaacggaa tttgtcacta ctgtgatctg ttttccttgc agaaaaggaa tcatttctaa
     6001 cgtagtagag ccagcgtcta aataaatgac atcttcgtca ttaatggttt cagctgccga
     6061 tttggcgatt tgctgttttt cgtgaacgtt tttgagtgat ttttcactca tattctgctc
     6121 aaaattcaat aattgctctt tcttagctcc gccatgaact ctttctagaa acccactatt
     6181 ctccagctct tgtaaatcac gtcttatcgt tgattcagaa gcattcaaaa ggtttacaag
     6241 atcttgtgat ttcacgattt tctgctgttc aagcaagtct aggatttttt gtctgcgttc
     6301 ctctgtaagc attttcacga tcacctcaat acaaataata gcatattttt tttctactag
     6361 caatataaat cagtcaaaaa cgttcataaa aattataaat cagtcaaaaa aaaaataaaa
     6421 aaccagtcag aaagactggt tttttatttt tttgtctatc atcaagccgt tttatcgttt
     6481 tttattttcg cttcttgttt ttttcatata aacagaagca ggttttcttt ccttatgaag
     6541 aagataataa taaagtccca tcccgccaaa catgaccaaa aaaccgatga ctgatcgaat
     6601 aggaaaatta ggaacaaaaa agagacagac ggataataat gatgccggaa ccattattcg
     6661 ataatagagt tgtttatcca ttttcttcac ctcatatttt ttgtaaagta atcaattgtt
     6721 ctaattctcg gtaatctaaa aattgcacac gcagatgctg ctcacctttc ttgacatcga
     6781 attggatact tcgccgcttt ttctttattc gtttgaaggt aacatcagag agtctatgtt
     6841 tgatttcctt taacggtaat tctgacaggg attctgaagg aacatagatt cttaaggata
     6901 aaagatactc ttgatatttt tcatctgtta cctctacttc tagatattcc ctaatataat
     6961 aacaataagc ttcgattcca ttatccaaag caacagtcgg atcgataact ttatagcaca
     7021 tcatgcgtga tcgattctcg tcaacccaag gaaaatacgc ctgagcattt agtgttccgc
     7081 gaacacgcct ttctttataa tctccttgtt ggatatcata actttttgtt acaaaaaatg
     7141 aagcattctt tttaggaacg ggttgcattt gtgcattctg atactggaca gattggatcg
     7201 tatggtcagt gtaaatgtat tcaacgaatg gcggtttcaa taaaccgaca ccattgtccc
     7261 aagcaagatg atcaaaccca agctggtccc taaaatagga ttgaaacatt tgattgtttg
     7321 gcttttgcca gttaaagggg tctcgataaa gctggataca tatgacttcg ttttctaatt
     7381 ccttcacttt ttctacttgt tggatgtcga gaaaaatcgg tttagggatg atttgatgat
     7441 aatagcgact ataatacatt tcccaacacg aagtgaaaca taaaccttgg taaaaaagat
     7501 cgtatcctgg aaattggttg caatcgacta ctacttcccc gttttgttgt ttcattttag
     7561 gtaaatgttc gatttcatcc tttttctcaa acaatagccg ttgagagatc tgttttgtat
     7621 tgtgatagat aaactcgcta taagctcgca tataggcgaa caccccatga gcaatcattt
     7681 ttttctgaat gaagtctcga atcaaaaatt gcttttcttt gaaaacagag atagacaata
     7741 cattttttat caataattga ttatcttcca agcgaaaact acattcgttc tcgtcatttc
     7801 ctacaactaa ccaactttcg tctcttaccg atgctagcat tattttttct tgaagctttc
     7861 gaagatcata cggatccgct tgttgtgtga aagagcggat aaatgttggc tggatcaaca
     7921 gacgtgtcaa aatagcccaa tcgcttattt cctgtttttt ttcttcttca tttaagtctg
     7981 tgagctggat agtcatcaat aatttatctt cgccaatcat tttctcacct ctttcaaatc
     8041 tttttataat ttaattataa ccaatgataa agaaaaacta aaactttatg tcttagaaac
     8101 caatttggac gattacatga tagactaagt aaaaagaaac aaagaggtgt ttacaatggg
     8161 gttgaaattt gaacgtacag ataatttaga taaacttttc gaatcatttg ctgtcgatcc
     8221 ggaaaaaaca gttgcaccaa aagaagagat cgatcgattt ctaaaaccac agccacaaaa
     8281 tgaaaagaaa aaagacaaga aacaaaagta agttaagctc aaaaaaa
//
LOCUS       DACEXQ010000113         8303 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.113, whole genome shotgun sequence.
ACCESSION   DACEXQ010000113 DACEXQ010000000
VERSION     DACEXQ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.113"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            47..679
                     /locus_tag="IXH04_001758"
     CDS             47..679
                     /locus_tag="IXH04_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAQ1852360.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGNQSYLDAKN
                     MVLRESHIQKLTMQKTGLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTTIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(681..1586)
                     /locus_tag="IXH04_001759"
     CDS             complement(681..1586)
                     /locus_tag="IXH04_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ1852361.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWEIALIISLVLSIWMLIALWRRIR"
     gene            complement(1671..2324)
                     /locus_tag="IXH04_001760"
     CDS             complement(1671..2324)
                     /locus_tag="IXH04_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ1852362.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVCV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVSI"
     gene            2646..3590
                     /gene="mvk"
                     /locus_tag="IXH04_001761"
     CDS             2646..3590
                     /gene="mvk"
                     /locus_tag="IXH04_001761"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAQ1852363.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            3583..4560
                     /gene="mvaD"
                     /locus_tag="IXH04_001762"
     CDS             3583..4560
                     /gene="mvaD"
                     /locus_tag="IXH04_001762"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAQ1852364.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKKGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            4573..5658
                     /locus_tag="IXH04_001763"
     CDS             4573..5658
                     /locus_tag="IXH04_001763"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAQ1852365.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            5655..6719
                     /locus_tag="IXH04_001764"
     CDS             5655..6719
                     /locus_tag="IXH04_001764"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAQ1852366.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKDPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAQEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(6968..7771)
                     /locus_tag="IXH04_001765"
     CDS             complement(6968..7771)
                     /locus_tag="IXH04_001765"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAQ1852367.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIRDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(7791..8096)
                     /gene="kduI"
                     /locus_tag="IXH04_001766"
                     /pseudo
     CDS             complement(7791..8096)
                     /gene="kduI"
                     /locus_tag="IXH04_001766"
                     /inference="COORDINATES: protein motif:HMM:NF002091.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
     gene            complement(8078..>8303)
                     /locus_tag="IXH04_001767"
     CDS             complement(8078..>8303)
                     /locus_tag="IXH04_001767"
                     /inference="COORDINATES: protein motif:HMM:NF018513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAQ1852368.1"
                     /translation="LWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKIIKEAIPSLLLI
                     VIIIGGILTGIFTAVEASAIAVIYKWVIRF"
BASE COUNT         2509 a         1741 c         1625 g         2428 t
ORIGIN      
        1 gattcccgct tctttgctat actaaagaaa aacgaggtga acccacatga aaaaaatcga
       61 acctattcca ccttctcttc ctcttcttac aaagggagag ttttatttag aagatgagga
      121 gatcttctct ggtcttctga tcaacggagg taaccaaagc tatctcgatg ccaaaaatat
      181 ggtattaaga gagagtcata tccaaaaact tacgatgcaa aaaacaggtt tacgtcattt
      241 tgaatgcagt aatgtcattt ttgaaaaatg tgatttttcc aatctggagt ggattggtgg
      301 cagtttccac caagtcgtgt ttcatcaatg taaattaaca ggtacgaatt ttgccgaaag
      361 ttatcttcga gactgtactt tcactgattg tattgcttcg atggcttcct ttagtagtac
      421 aaacttgaaa gctgtctcct tcgatcattg tcaattagaa gacagcgaat tttatgaggt
      481 cacttggaaa aacctttttt tgtctgacaa tcagttaacc ggttcgaact ggtttcgaac
      541 ttctctgaag ggattagact ttacgactaa tttcttcaca acaatcgctt tatctcaaga
      601 ttctttggcg ggactaatcg tcaatcaaga gcaagcactt gtgatcgccc aagcccttgg
      661 tttgcagatc aaagactaga tcaacgaatc cgccgccaca gtgcaataag catccaaata
      721 gataagacca aactgatgat caaggcgatt tcccatccga aaacggaatg ggtaaagggt
      781 aactggagat tcattccgaa gaatcctgtc acaatagttg gaacggtcaa gatcagcgac
      841 cagaccgtca aaaatttcat cgtgtcattc aagttgttat tcagcaaatt attataagtg
      901 ccagagagct ggtctaaaat ctgcgaagct aagttcgtca tttcaactgc ttgtcgtgct
      961 tcgatcagtg catcgtccaa ctcttctttt tccttttctg acaacttgcg ataaatagcc
     1021 agtgccttga tttgttctaa aagtactaca ttttgttttg ttcctgtaac taagtaaacc
     1081 aaaccgatct ctaaatctga aagttgcagc agattcttgt tcgttgtttt ttctcttaat
     1141 ttcagattaa gccgctgacg ttcactattc acttcctcga ttaaaggaaa aaaggaatca
     1201 gaaatcaaaa acaatgtacg aaacaataaa ctgtatacac tcatgtctgg catccgttcc
     1261 aaaagcgatc gaattagtcg aatcacgtac tcggtattcg gcgtggtaaa tgtaaacaaa
     1321 ccgtcttctt ttaaaataaa agcgatagga ctagtttcat aatgattatc gactttttgc
     1381 tggtgcggaa catttgatac aagcagtaac gcttcatcaa aagaatcgta ctctacgcgc
     1441 gcccgttcat ttttatctag agaataagtc agcatctcgt cactaatgcc atattcttct
     1501 tttaagtttt taacagctag cacatcgttt tgctgtacat tgatccaaaa attacggtct
     1561 tgattaaaat agtgtttttc ccgcatactc tatcctcctg acatttgttt ccagtataaa
     1621 gaaaaaagtc gctcaaataa aggtttaccc tttatttgag caactgtaaa tcaaatggat
     1681 actttggttt ggcgaatctc tgctaatttt tcttcgatca ttttcaagac ttcttctttt
     1741 gcttgaggat cttccacaaa attatagcgg tctccatcga tttggatttt tggactttct
     1801 ttgtattctt cataccattg atcataacgt ctgttcaatt ctttgtaata atcgtataga
     1861 cttgggtcaa aggacaattg ttcatacgaa cgaccacgtt tttcgatacg ctctaacatc
     1921 gtatcaaaag atacacgaat atgaactaat aaatccggat gttttttatg ggcagcatag
     1981 ggaagttctt ccatcatgtt ttccagcagt tcatcataca cacaaacttc tgtttctgtt
     2041 gctcttccta gatctgcatt tagatggaaa agcagtgaat cttcataaat cgaacggtcc
     2101 aatacattat tgtcatcttt cattgcttgt ttgatgcttg caaaacgttt attcaaaaaa
     2161 taaatttgca gcaaaaaagc atactgttca gggtttgcat aaaataaggg caagacttca
     2221 ttatcatcaa tcgattcata aaatgcctga ctatcaaagt gttcagccat catttctgtt
     2281 aaacttgatt ttccggcacc aattgtacct gctaaaacga tcacgctcat ttacctccag
     2341 ctcacttcat cactaaatat tgtgtttaac tccaaccttt tttaaatata cccccaatat
     2401 ctagtagtgt caattgtatt ttttctagtc aaagaagaaa aaaagtcaaa aaattcttaa
     2461 gaaaaaagtg ttttttcttt agaaaacgta ttcttagttt tacttctgag atggttttag
     2521 taaaataaag gaggaatgtt ttttgagacc cttagtggac aaaaaaattc gtgaagcagc
     2581 gcaactcgtt ttgggcgctt tattttttgc ttaaaaatca cttttacgtg agaaggagtt
     2641 agattatggc aaactatggc caaggagaat caagcggaaa gatcatattg atgggcgagc
     2701 acgcggttgt ttatggagaa ccagcgattg cctttccttt ctatgcaaca aaagtcaccg
     2761 cattccttga agagctggat gcaatggacg atcaactggt ttcttcctac tattcaggaa
     2821 atttagccga agctcctcat gcattaaaaa atatcaaaaa attattcatt cacttaaaaa
     2881 aacagcatga catccaaaaa aacttgcaac tgaccattga aagcacgatt cctgctgaac
     2941 gtggaatggg atcaagcgct gcagtcgcca cagcagtcac tcgtgctttt tatgattact
     3001 tagcatttcc tttgtctcgt gaaatactat tagaaaatgt ccagctttcg gaaaaaatcg
     3061 cccacggtaa tcctagtgga atcgatgcag ccgctactag cagcttgcag ccgatttatt
     3121 ttacaaaagg gcatcctttt gactactttt ctttgaacat cgatgctttt ttgattgtcg
     3181 ctgatacagg aatcaaagga caaacaagag aagccgtcaa agatgtcgct cacctctttg
     3241 aaacccagcc tcatgaaact ggacaaatga tccaaaaatt aggatacttg acgaagcaag
     3301 caaaacaagc aatcattgag aattcaccag aaacgttggc acagacaatg gatgaatcac
     3361 agtcacttct ggaaaagctg acaatcagca atgattttct taatctattg atccaaacag
     3421 caaaagatac cggggcattg ggtgccaaat taactggcgg tggacgcggt ggctgtatga
     3481 ttgcattagc acaaacaaaa acaaaagccc aagaaatcag tcaagcactt gaagatgcgg
     3541 gtgctgctga aacttggata caaggattag gagtacatac ctatgtttaa aggcaaagca
     3601 cgcgcatata ccaacattgc tctaatcaaa tattggggta agaaaaatga agaactcatc
     3661 cttccaatga acaacagcct ttcgttaaca ttggatgcat tttatacaga gacagaagtc
     3721 atcttttctg atagctatat ggtagatgaa ttttatctag atggcacctt acaagacgaa
     3781 aaagcaacaa aaaaagtcag tcagtttctt gacctttttc gtaaagaagc cggcctctca
     3841 ctaaaagctt cagtaatcag tcagaatttc gttcctaccg cagctggatt agcctcctct
     3901 gccagcgggc tagctgcttt agcaggagct tgcaatactg ctcttaagct tggattagac
     3961 gatctctctc tttcaagatt tgctcgacgc gggtctggtt cagcttgccg aagtattttc
     4021 ggtggtttcg ttgaatggga aaaaggccat gacgacttaa gttcttacgc taagccagtc
     4081 ccttccgatt ctttcgaaga cgatttagca atggttttcg ttttgatcaa cgaccagaaa
     4141 aaagaagtgt ccagcagaaa tgggatgcgt cgaacagtcg aaacatccag tttttatcaa
     4201 ggctggttag attccgttga aggggatcta tatcaattga aacaagcaat caaaacaaaa
     4261 gatttccaac ttctcggaga aacgatggaa agaaacggac taaaaatgca tggaacgact
     4321 ttagctgccc agcctccatt cacttactgg tctcctgaca gcttaaaagc gatggatgcg
     4381 gtgcggcaat tgagaaaaaa aggcattcca tgctacttta cgatggatgc tggccctaat
     4441 gtcaaagttt tagtggaaaa cagccattta tctgaggtac aagaaacatt taccaaatta
     4501 tttagcaaag aacaagtaat tactgcccat gctggtccag gaattgcgat tattgaataa
     4561 agggagattt ttatgattga agtatctgca ccaggcaaac tctatattgc cggagaatat
     4621 gcagttgttg aaacaggaca tccagcagtt atcgctgcag tcgatcaatt cgtaacagtc
     4681 actgtagaat ccgcacgaaa agtcggaagt atccaatctg cacaatatag tgggatgcct
     4741 gtacgttgga caagacgcaa cggagaatta gttttagata ttcgggaaaa tccttttcat
     4801 tatatccttg ctgctattcg tttgactgaa aagtatgcac aagaaaaaaa cattctttta
     4861 tccttttatg atctgaaagt aacaagtgag ttagacagtt caaatggccg gaaatatgga
     4921 ttgggttcta gcggtgccgt gaccgttgca acggtcaaag ccttgaatgt tttttacgcg
     4981 ttgaatttat ctcaattgga gattttcaag attgcagcac tagccaattt agcagttcaa
     5041 gataatggtt cctgcggcga catcgctgct agctgttatg gtggctggat cgctttctca
     5101 accttcgacc atccttggct ccaagaacaa gaaactcagc attctatcag tgagttactt
     5161 gccctggatt ggccaggtct atccattgag ccattgattg ctcctgaaga tttacgttta
     5221 ttgattggtt ggacgggtag ccctgcctct acttctgatt tggtcgatca agttcaccgt
     5281 tcgagagaag acaaaatggt ggcttatcag cttttcttaa aaaacagtac agaatgtgtc
     5341 aacgaaatga tcaaagggtt taaagaaaat aatgtaacgt tgatccaaca gatgattcga
     5401 aaaaaccgac aattactgca tgatttatct gcaatcactg gggtcgtcat cgaaacgcct
     5461 gctttgaaca aattgtgtaa tttagctgaa cagtatgaag gagccgcaaa atcttctggt
     5521 gcaggtggtg gcgattgcgg aatcgtaatt gttgaccaga aatctggcat ccttccttta
     5581 atgagtgcat gggaaaaagc agaaatcact ccactgccgt tacatgtcta tagcgatcaa
     5641 agaaaggaaa accgatgaat cgaaaagacg aacatgtttc actagctaaa gcctttcatg
     5701 ataaacaaaa aaacgaattt gacttcgtac ggatcatcca taaccctttg ccgcaaatag
     5761 cagtttctga tgtcgatcta agtacacagg cagttgggtt tacactaagc agcccatttt
     5821 atatcaatgc gatgactggc ggaagcgaaa aaacaaaaaa aatcaaccaa gatcttgcaa
     5881 tcgtcgcaag agaagcagat ttgatgattg ccactggttc agtcagcgct gccttgaaag
     5941 acccttcttt agccgatact tatacgatta tgcgacaaga atatcctcac gggaaaatca
     6001 ttgccaacat cggtgcaggg acttctgtcg aaagagccca agaagctatc cgattgtttc
     6061 acgcagatgc cttacagatt catttgaatg cacctcaaga gttggttatg cctgagggag
     6121 accgtgattt taccaattgg aaagtactta ttcaagagac tcaaacagcc attgacgtcc
     6181 ccctcatcgt aaaagaagtc ggttttggca tgacaagaga aacgctcaat gatctagctg
     6241 ctttaggagt tcatacagtt gatatcagcg gtcgaagcgg aacaagcttc acccagatcg
     6301 aaaacgcccg tcgctccaag cgggaactga gttatttagc tgactggggg caatcgacag
     6361 tctcttcatt gcttgaagca aatgaagcag acacttctat ggaaatccta gcctccggag
     6421 gtatccgcaa tgcctatgac atatttaaag ccctttgtct cggcgcaaat gcagtgggta
     6481 cttcaggaac cgtactgacg catctgatga atcacggcgt agaagaaact attatactga
     6541 tgaagcaatg gcaagaagag cttcgtctgc tttatactat ggtcggcgca acgaataccg
     6601 cggccctaca ccaacaatca ctgatctttt ccggaccagt gaaggattgg tgcgaagcaa
     6661 gagggatcga cttagtgaag tatggaagac gcacagaaaa gagcgtggga caaaagtaaa
     6721 gaacactttt atcccacgct ctaaacacgt ataaacggcg gaagcaaaag caactccttc
     6781 ggaaataagc tgaaattcac aaaaatttga aaagcaattt tcgtgaattc cttcttattt
     6841 cttggagtta aacgcttctg tcccagcctc ttttttcgaa taaatgatgt ttaatcagct
     6901 gtccttcggt attcgttcaa gccgctgatt tcacaacctc tccactaaac ggtgtactaa
     6961 agctctttta aggttgtttt ccgatatagg caagtatgcc tccgtccaca tacaagacat
     7021 ggccgttaac gaaatcagat gcgctagaag ccaagaagac agatggtcca gccaaatcga
     7081 ttggctcgcc ccagcgtgct gctggcgtac gtccaacgat aaattgatcg aatggatgac
     7141 gttcaccgtt ttcttgtgtt tcgcgtaatg gagcagtttg cggtgtagca atataacctg
     7201 gtccaattcc attacattgg atattgtatt gaccgtattc agaagcgatg tttttcgtca
     7261 gcatttttaa tccgcctttt gccgcagcat aagcactgac cgtttcgcgt cctaattcac
     7321 tcatcatgga gcagatattg atgattttac cgccgccttt ttcgatcata tcccgaataa
     7381 ctgcttttga aacgatgaat ggggcattta aatccacgtc gattacttgg cggaagtctg
     7441 ccgcatccat ttcgatcatc ggtgtccgtt tgatgatccc tgcattgttg actagaatat
     7501 caactgaacc tacatcttct ttgatctgtt gaaccatcgc ttgaaccgct tcttcatcgg
     7561 tcacatcgca gacataacct tttgcatcga tgcctgcttc tttataatgc gctatacctt
     7621 catcaactga ttcttgattc aagttattaa atacgatttt tgctcctgct gtagctaaag
     7681 agcgtgcaat ctcaaatcca ataccataga cagcaccggt gactaatgct acttttccat
     7741 ctaaacgaaa catatccata ctaaattcca tcattttctc tcccttatgc ttattttaat
     7801 tgatccattt gcaccatatc catgtctgta taggtgatat tttctccgca cattgcccag
     7861 atgaatgagt aattgcttgt acctacacct gaatggatcg accagcttgg cgaaataatg
     7921 gcttgttcat tgtctacgac taaatgtttt gtttcatctg gtttgcccat catatggaac
     7981 acacgtgtat cttcattggc ataatcaaaa tagacataag cttccatacg acgttcatgt
     8041 gtatgacaag gcatcgtatt ccaagcactt cctggttcta aaatcgtata acccatttat
     8101 aaatgacagc tatcgctgaa gcttctactg ctgtaaatat ccccgtcaag ataccaccga
     8161 taatgatgac aatcaataga aggctaggaa tcgcctcttt tattattttt ccagcatgag
     8221 ccatctgtcc tttttcgaca acagggtatt tattcttttt agcaacgatg aatgctacta
     8281 gcatgattcc tagcgcccac aaa
//
LOCUS       DACEXQ010000114         8243 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.114, whole genome shotgun sequence.
ACCESSION   DACEXQ010000114 DACEXQ010000000
VERSION     DACEXQ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8243
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.114"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            172..1365
                     /locus_tag="IXH04_001768"
     CDS             172..1365
                     /locus_tag="IXH04_001768"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAQ1852369.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1495..2967
                     /locus_tag="IXH04_001769"
     CDS             1495..2967
                     /locus_tag="IXH04_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1852370.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3050..3838
                     /locus_tag="IXH04_001770"
     CDS             3050..3838
                     /locus_tag="IXH04_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAQ1852371.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(3905..4573)
                     /locus_tag="IXH04_001771"
     CDS             complement(3905..4573)
                     /locus_tag="IXH04_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ1852372.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            4688..6400
                     /locus_tag="IXH04_001772"
     CDS             4688..6400
                     /locus_tag="IXH04_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1852373.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6403..8175
                     /locus_tag="IXH04_001773"
     CDS             6403..8175
                     /locus_tag="IXH04_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1852374.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2524 a         1382 c         1811 g         2526 t
ORIGIN      
        1 ttttttggac agttctaatg taaaatattt ttatattttt taagcaaaaa aatgaaaaaa
       61 tacgagcaga aaactgtttt atctgctata cttggttcgt tgcaagttcc cgaaaggatc
      121 cgcaggacgg agatgccttg taaccgaatt taactagggg gaatcattat aatggtagaa
      181 agacacttat ttacatcaga atctgtttct gaaggacatc cagataaaat tgctgaccaa
      241 atcagtgatg caatcttgga tgcaatttta aaacaagatc caacagcacg agtagcgtgt
      301 gaaacgtctg taacaactgg tcttgtttta gtatttggag aaatatctac gacggcatat
      361 gtagatattc aaaaagtcgt acgggaaaca ataaaagaaa ttggttatac acgtgcaaaa
      421 ttcggatttg atggagatac agcagctgta ttggttgcga ttgatgaaca atctcctgat
      481 attgctcaag gagttgatga agcacttgag atccgggatg aagataaaaa agacgtatta
      541 gatgagattg gtgctggtga ccaaggttta atgtttgggt ttgctgtaga tgaaacacca
      601 gaattgatgc ctttaccaat cgctttgagt catcgtctgg tacgacgctt ggcagatttg
      661 cgtaaatcaa atgaattaac gtatttacgt ccagatgcaa aatctcaagt aacggttgaa
      721 tatgatgatc aaggacaacc ggaacgcgta gatacaatcg ttatttcaac acagcatgat
      781 gatgcagtag ataatgaaac aattcgtcat gatgtcatcg aaaaagtagt gaaggaagtt
      841 attccagctg aattattaga tgatcaaacg aaatattata tcaatccgac tggccgattt
      901 gtcattggtg gtcctcaagg ggatgccgga ttaacaggaa gaaaaatcat tgttgatacg
      961 tatggcggtt atgctcgtca tggtggcggt gctttttctg gtaaagatgc gacaaaagtt
     1021 gaccgttctg ccagctatgc tgcacgttat attgctaaaa acattgttgc agcagggctt
     1081 gctcgcaaag cagaagtaca gctagcttat gcgattggtg ttgctcaacc tgtttcgatc
     1141 tcaatcaata ctttcggtac tggaacggtt ccggaagaag aactgattgc tgcagtgaga
     1201 gaaaacttcg atcttcggcc agcaggaatc atcgagatgc ttgatttgcg tcgtccgatt
     1261 tacaaacaaa cagctgctta cggtcatttt ggtcgtacag atgtagattt gccttgggaa
     1321 catacggaca aagtagatgc gttgaaagca agtttagcaa aataagttat attcagctgg
     1381 aacgatcgcc aagtggtggt tgttccagct ttttttatgg cagttaacct cttgaaaaag
     1441 atagtaatta tttatctatt tttagatcct gtcataatga aagggaggaa taagatgaaa
     1501 aaagaaacaa atgttacttt ggtcacagtg agtattttca ttgcaacgtt catgacggct
     1561 attgaaggta caatcgtaac aaccgctatg ccaacgattg tcggttcatt acatggaatg
     1621 gaaatcatga actgggtatt ttcgatttat ttgctgacaa acgctatgct tactccgatt
     1681 tacggaaaac ttgcggataa agtgggacgg aaaccaatct ttatttttgg gacattattg
     1741 tttattatcg gttcgttatt ttgcggttta tctcaaagca tgattgggtt gattgctgct
     1801 cgtgcgattc aagggatggg tgctggggca atgatgccag tagctttgac gatcatcggc
     1861 gatctttatt ctattgaaaa gcgagcaaaa gttttaggtt tgaatagttc agcctggggg
     1921 attgccagtg tttttggacc gcttgcggga ggattcatcg ttgaaacgat cagctggcac
     1981 tggatctttt ttatcaatgt gccgatcgga attctattaa ttattttgat ttggctatat
     2041 ttaaatgaac caaaaatcat acatgagtct aaaccaatgg atatttttgg tagttttagt
     2101 ttgatggcca tgcttgtctc attgctgtta gggttccaat tattaggaga cgaaggaatg
     2161 agtccgcttg tttctgcttt gctgctaggt agtatcgtaa tgttaggtat ttttgttttt
     2221 gccgaaaaaa gagctaaaga tcctgtcatc atgatgcatt tattcaaaaa tccactattt
     2281 gttgtggtca acttggctgc agcattggct agtggttttt tgatgggaat cgatgtttat
     2341 atccctatgt ggatgcaagg tgtattaggg ctgaatgcag caattggtgg gctggtatta
     2401 gcaccattat cactagtctg gatggtcggt tctttccttg caagcaagtg gatgactcaa
     2461 atgaaaactc attgggtctt gagaattgga ctactaatcg cagctattgg gggcgcttgg
     2521 ttgtatctta tgcctttcag aagtaactat ttatggtttt ttgctatatc aacggtcatc
     2581 ggtataggct taggtgtgac catgactacc tcgactgtca gtgcacaaaa tagtgtatct
     2641 cgtgaccagc ttggtgtagc aacttctttt aatacgttag ttcgaacaat tggtcaaaca
     2701 gtcatggttt ctatttttgg tttattgata aatggggtaa ctaaacgaga acttgcagca
     2761 gcaaatttaa caaatgatgc agatatcatg aataaactgg tgaatccaca aacggctaaa
     2821 tatattgatg cggatatttt gttaccatta agaaaaattc tttatgatgg gttacattcc
     2881 gtttatctag ttggcttgtt actcgtgatc attgctttgg tcttaacgct tttcgttcat
     2941 gaaaaaagtg gtaaaataaa acagtagttt cggttaagat atgtacgtag ccgaatcatt
     3001 caaatttaaa gttattcaaa aataacttct tactaaggaa ggcgaaaata tggcatttat
     3061 tcaagcaaat atttattcga atgtgttaga aatggaagtc aatctaaatg tgatactacc
     3121 gcaaacaaca aaaaaggtga ttggcaccag ttcagagcaa gagacaacgg atatacctgt
     3181 tttatattta cttcatggaa tgggtggcaa tcatagtgta tgggaaagac gtacgtcgat
     3241 tgaacgttat gtagcaaact acggattagc tgtcatcatg ccatctactg atcttggctg
     3301 gtacacagat acagtttatg acatgaagta ttggacattc attgcagaag agctacctga
     3361 gatttgtcat gaattattcc cgcaattaac aagaaaaaga gaaaaaacat atgccgctgg
     3421 attatcgatg ggcggatatg gcgcgttaaa actaggcctt gcaaaatcag aaaatttcgc
     3481 agcagtaatc tctctttcag gcgctgtttc tgtaggaaat cgaatggatg atcttttgat
     3541 ggttcgtaaa gcatcttttt gggaagggat ctttggacca ttggatcaaa tcgaaggttc
     3601 aaaaaatgat ccgcaatact tattgaaaga actagtaaaa agcggaaaac cagttccaag
     3661 attttatatg gcttgtgggg aatctgattt tctttatcag gcaaatcagg aaatggtatc
     3721 tgctatgcag gaacaaggct tggcggtcac ctttgaacaa ggacctggcg atcataactg
     3781 gatcttctgg gaccaatgga ttcaacgtgc attggcgtgg ctaatggaag aaaaataaaa
     3841 aaagagtctt aaaaagatag ctcctataga gatcacaaac atagtgatct ctataggagc
     3901 ttttttactg ctcgttttct cttcttttat tttgttggca taagccttca atataggttt
     3961 gtgcactcgt tagaaagagt gctttttcat tcaattctgc atgggactgc atactttctt
     4021 taaatccctc taattgattt tcaactacat aattacgcat aaatagaact gtttctttca
     4081 aaaattcatt tccgtccata attttttgtc gaagctgctg ttcatgctgc tgatccatta
     4141 actgtccaat caataaattg tggagagagg caatggcctg atgactttgg gtgactgaaa
     4201 atccttcacg aacaagtaat tggatcattt gctccaaatg ttctaatcga ctcggaaaag
     4261 cgggcacagt attgatttct aattctgccc cacaagggaa acgtgtataa aggtcatagt
     4321 aattttccat atactgcagc aactgttcat tccaaggcaa agttggatct ggaagcgcta
     4381 attcttcttc cattgtttca gccatttttt gcaagagctg ttgtttgttt ttgaaatacc
     4441 aatataaagc aggtgcttga acatgaattc tgtttgcgat gtttcgcatg gaaagctgcg
     4501 aaagagcttg tgtttcagcc aggatatcaa aggcggcctg aatgatgatt gcttgagaaa
     4561 gctttatttc cattttatca acctcgtgta ttgcattatc tttttaaaca tggtaaattg
     4621 ttagtcggtc taattttaca gtgttaaatt agtcctgtca atttaaacaa aggggtgtaa
     4681 tcaacgaatg aaattaatct tacgacatgc gaaaaaatat aaagtagctg tttttatttc
     4741 cttgctgtcc gtagcagtca tggttgcagc agcattatgg caacctaagt tactacagca
     4801 agtattggaa gctattatta cagaagataa tgatgaaatg aaaacaattg gtatttacct
     4861 gatcagtctg gctttgctag gattagccgc aggtgtaacg aacacgattt tctctgcaaa
     4921 agtagctcaa ggcgtaagtg cagatatccg agaagaagca tttcgaaaga tccagacatt
     4981 ttcatttgga aatatcgaac agttctctgc tggaaatctt gttgttcgtc taacaaatga
     5041 tatcacgcaa atccaaaatt tagtgatgat ttctttgcaa tcactttttc gaattccatt
     5101 cttatttatt ggtgccttca ttttagctat gatcacgatg ccgcaattat ggtggatcat
     5161 cgtagcgctt atcgttacgg tatttctgat tacggcactt tcatttacaa ggatgggcaa
     5221 gcacttcatg atcattcaaa aactgattga tcgtgtgaat agcattgcaa aagaaaactt
     5281 actagggatt cgtgtagtga aatcttttgt acaagaagaa aaccaattga agtcattcag
     5341 caaagtaagt gaggacttaa caaaacacaa catcatagtc ggtacattat tttctgtgat
     5401 gatcccgtcc ttcatgctgg ctgccaattt ggctgtggtt ggggcgatct tttttgtgag
     5461 cgatcttgtt aaagatgatc ctacgttaat tggtggaatt gcttcattca tgaactactt
     5521 gatgcagatt atgatggcaa tcattattgg cggaatgatg atgatgatga cttcaagagc
     5581 agccgtatcg ctgaagcgga ttgcggaaat tttagatgct gaaccagatc ttacttattt
     5641 agacgttcca gaacaagagt tgacgggttc tgtcactttt gaccatgttt cttttcgtta
     5701 tcctggtgag gatacagata cgcttaaaga tatcagcttt tctatcaaac ctggagaaat
     5761 ggttggtatc gtcggggcga ctggtgccgg gaagtcaaca ctagcacaat tgatccctcg
     5821 tctatttgat ccgacagaag gcaaagttga agtaggtggt gtagatcttc gtcaagtaaa
     5881 tgaaaagagt ttaagacaaa cggtttcctt tgttttgcaa aaagcaattt tattctcagg
     5941 aacgattgct caaaacttgc gccaagggaa aaagaatgca acagaaaaag agatggaaca
     6001 tgcttcttct attgctcaag caaaagaatt catcgaaaag ttatctgatc gttacgaagc
     6061 accaatagaa gaacgtagca gtaacttttc tggtggtcaa aagcaacgtt tatccattac
     6121 tcgtggcgtt atcggtaatc caaagatctt gatcctagat gacagtacga gtgcactgga
     6181 tgctcgctct gaaaagctgg tccgagaagc actcgatcga gatttgaagg gaacaacgac
     6241 gattgtcatc gcacaaaaaa ttgcttctgt tgtcaaagca gaccgcatct tagtattgga
     6301 tgaaggcaga ttagtcggcg aaggaaccca tgaggaattg gtagctggaa accgtgtcta
     6361 tcaggaaatc tttgaaactc aaaaaggaac ggaggagtaa gtatgactga tctaataaaa
     6421 gcaagtaaat ttttctatca ctatttgaag cggtacaaac tctccttttt ctttatcttt
     6481 atcacagtga tcattgctac ctatctacaa gtaaaggcac ctcaatacat cggggaagct
     6541 tttcaagaat tagctaatta cgtaggaggc ttgatgcaag gcgtagacga taaaagcaaa
     6601 tttgtttctg taatatggaa actcttgctg ttttatgtac tagctagtgc agccaacttt
     6661 atctatagta ttttgttcac acaagtagta gggaagtcaa cgaaccgaat gcgtatcggt
     6721 ctattcaaca aattggaaaa attgacgatc cgtttctttg attctcatca agacggtgaa
     6781 attttaagtc gttttaccag tgacttagat aatattcaaa atagtttgaa ccaagctttg
     6841 cttcaagtca tcacgaatgc tgttttactt gtcgggatct tgatcatgat gttccgccaa
     6901 aatgtagagt tagcttgggc tacaattgct tccactccag tagccgtttt gatagctgtc
     6961 ttgattatcc gcaaagcacg gaaatatgtg gatcttcagc aagatgaagt ggggaaacta
     7021 aatggttata tggatgaaaa aatcagcgga caacgtgtca tcatcacaaa tggcttacaa
     7081 gaagaaacga ttgaaggatt cttagagcat aatcaaagtg ttcgcgaagc aaccttcaaa
     7141 ggacaagctt attctggtct actatttccg atgatgcaag gaatgtcact ggtcaataca
     7201 gcgatagtta tctttttcgg cggctggtta gcattaaatg gtgatttgga acgttcggtg
     7261 gcattaggct tagttgtgac attcgtccag tattcacaac aatattacca accactgatg
     7321 cagatttcat ctggatacag catgatccaa ttagcgatta caggggcaag acgattgaat
     7381 gaaatatttg atgaaaaaga tgaagtcaaa ccagaaaacg gtaaagcatt tgatggtgtt
     7441 gaaaaaggtc ttgcgttgca gcacgtagat tttggttatt ctccagaaaa actagtcttg
     7501 aaagatgtct cgatcaatgt ggacaaagga gaaatggttg ctcttgttgg tccaacagga
     7561 tcggggaaaa cgacgatcat gaatctgcta aaccgttttt acgatgtcaa tgatggttca
     7621 gtagtcatcg atggaataga tatccgagat tatgatcttg atgcattacg tagacatgta
     7681 ggaatcgttt tgcaagaatc tgtattgttc tccggaacga tcaaagacaa tatcacattc
     7741 ggcaaaccgg acgcttcaga tgaagaagtc atcaatgcag caaaacaagc gaatattcat
     7801 gacttcatca tgacgttaga aaaaggttat gatacagaga tatccgaaga aaataactta
     7861 ttcagtacag gtcaaaagca gttgatcagt attgctcgaa cgatcatcac aaatccatct
     7921 ttattgattt tggatgaagc aacaagtaat gtagacacgg taacagaagc aaggatccaa
     7981 aaagcaatgg atgaagcaat caaaggaaga accagctttg tgattgctca ccggctgaaa
     8041 acaatcttga atgcagatag aatcgttgtc ttgcgtgatg gggaagtcat tgaagaaggc
     8101 agtcatcatg agttattagc agaaaatgga ttttatgcgg aactttatca taatcaattt
     8161 gtatttgaaa gttaaattaa aagaacctcg ttataacggg gttcttttta tatattttat
     8221 gttgtttgtt tttataaggg gca
//
LOCUS       DACEXQ010000115         8219 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.115, whole genome shotgun sequence.
ACCESSION   DACEXQ010000115 DACEXQ010000000
VERSION     DACEXQ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8219
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.115"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..101)
                     /locus_tag="IXH04_001774"
     CDS             complement(<1..101)
                     /locus_tag="IXH04_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1852375.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQA"
     gene            409..1215
                     /locus_tag="IXH04_001775"
     CDS             409..1215
                     /locus_tag="IXH04_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1852376.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            1227..2447
                     /locus_tag="IXH04_001776"
     CDS             1227..2447
                     /locus_tag="IXH04_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1852377.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     NKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            2437..4023
                     /locus_tag="IXH04_001777"
     CDS             2437..4023
                     /locus_tag="IXH04_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ1852378.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVVQTSIAQPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSEAT
                     ERMVFRFAQQPSFIEFSAEAAGEKLIFIDSNAVVKTVNDCYLFDGKEIPEFALTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENQHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKKTSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSRDEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            4062..6200
                     /locus_tag="IXH04_001778"
     CDS             4062..6200
                     /locus_tag="IXH04_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1852379.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKIRLSGKMRGKAILDFQTAKHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKTIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALEMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNIHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKKLGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPSYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            6488..7879
                     /locus_tag="IXH04_001779"
     CDS             6488..7879
                     /locus_tag="IXH04_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ1852380.1"
                     /translation="MNKNGEWNAARRIFIIIMDVLVLNVSIYLSFLLKFQGEVPARNL
                     ETFQHSAVFISIIFIALNILLGAYVFYSRVISDIVFITVIGQVLIALGIMVVTFAGRW
                     FAFPRGVIVLSFILGTILLSVYRIVVYKLYLRVSRDKKVLIVGLEKDVAPAIQNFQSK
                     KNNKHKVEAVVIDKYYENTKKLIDDIDIVYLASKIPEDEKINIYQLVTKKEKKLFLNT
                     TFENLTMINPNIMNFEDESIIEASGFKIQPEYELFKRLFDIIVSLILLILASPFMLIT
                     AILVKTTSPGPIIYKQVRITKNQKEFSIYKFRSMSVTAEAKSGPVFAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNEENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLLLDLKILLQTIKILFDKMSSQGLDEDENIRS
                     LAEIDFSQEKIYR"
     gene            7938..>8219
                     /locus_tag="IXH04_001780"
     CDS             7938..>8219
                     /locus_tag="IXH04_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1852381.1"
                     /translation="MLLSLYDKEKPEYLKEALTSIFEQTVMPKEIVLVYDGPINEKLQ
                     VVVTYFQQKDPHLFTVVKLEKNQGLGIALAIGLEHTKNRLVARMDTDDIM"
BASE COUNT         2791 a         1308 c         1654 g         2466 t
ORIGIN      
        1 tgcgcttgaa cgcctttata ttctttttca atcagccatt cttcatcaaa aaataaatgt
       61 ttatctgtta atccgagtaa ttttctagta tgattatcca ttgagagact cctttggtag
      121 gatgacgttt tggtcgacta aattttacca aaagtgagtc tctttttgag tttaaacgaa
      181 gaaaaagtgt tagtagaaaa tcgccttcga ttttccacca acacaaaata ttatagaacc
      241 ccttttttgg tttattgcaa ttgtctagca tcttctgtaa aatagaaacg ttagagaaaa
      301 tcttgtatca tagaaaatta agaaagaaat aagaacgcag ctcggtacca aaaatatggt
      361 aaaatggcac ttgactgtaa ttcactgaaa tcgcttggag gaacacaaat gtttaaagat
      421 ttgtatttat ttatgaaaga catttatcag aatagaaaat tactgctaca gttttcgatc
      481 aatgacttca aatcaagata tgcaggatcg tttttaggaa tcttatgggc gtttatcaat
      541 ccgctattta ctgtattgat ttattggctg gtatttggtg ttggactgaa agcacaaatg
      601 atcgatgcaa aatacccatt tatcgtatat ttagttaccg ggatgattcc ctggttcttc
      661 ttctcagatg catttgcatc aactacggtt gtttttcggg aatatactta tttagtaaag
      721 aaagtcgtat tcaatattcg tatcttgccc acgacgaaaa tcctatccaa tctgtacacc
      781 catttattct tcattctgat tggcatagct gtcgcgctta taaatggtat atatccttca
      841 gcaatgtctt tacagctgat ttattattta ttttgcatgt gtgctttctt gacaggattg
      901 acttggctga cggcatcgat ccaaccattt ttacctgata tcatgcaatt aattacgatc
      961 gtgctgcagt tgatcatgtg gacattaccg atattatgga gccctaatca gtttgattca
     1021 ggtcttgtaa gcatattgaa attaaatccg ctgtactatg ttgttcaagg ttatcgtgag
     1081 tcatttttaa gtgaagcttg gttctgggag catggaacat atactttgta tttctgggtt
     1141 ttcacgttag taatgttcgt aatcggctct gcagtgttcc gtagattgaa accacatttt
     1201 tcagatgtgc tgtaaggaga atagctatga cagaatatgc aattagacta aaagatatca
     1261 caaaatctta taatatgtac gcaaaacctt cggatcgatt tcgtgaagcc ctcaatcctt
     1321 ttaagaaatc ctatcatgat gtattttatg cgttgaaaaa cgtgaatgta gaaataaaaa
     1381 aaggtgagat gatcggtttc gtgggagaaa acggatcggg aaaatctaca atgttgaaaa
     1441 tcattacggg agtactgaca ccaacatccg gcacgatgga aatcgaagga aaaatctcag
     1501 ctttgctaga actaggatca ggattcaatc ctgagtattc tggctatgaa aatatctatt
     1561 taaatggaat ggttctagga ttttcaagag aagaagtcga tgaaatggtc gatgatatca
     1621 ttgaatttgc tgatattggc gaccatattt atcaacctgt aaaaacctat tcaagtggga
     1681 tgttcgttcg tctagctttt gctgtagcga tcaatgtgaa tcctgatatt ttgattgtgg
     1741 atgaagcatt ggcagtagga gatcttgaat tccaactgaa atgtatggaa aaatttacag
     1801 aaattaagaa ctcgggaaaa acgattttat tcgtttctca tgatattagt tcgattcgcc
     1861 gtttttgtga tcgttcctat tggttgaaaa atggagaagt tgtcgaatat ggtgatacga
     1921 tggaagtaac gacgaattac gaaaactttt tgaagaaaaa atcagttaaa acagtagatc
     1981 gaaataaaaa ccaagtagaa cgcttcgcag ctccagatat cgtagacgtg acgaaagccg
     2041 aattgttgga taaaaataaa aatccagtgg atatgctgga acaaggagaa aaactatacg
     2101 ttaaagtaac ctatgaagta aaagacgata caatcaaaaa tccagtttta ggaattgctc
     2161 tgcgtacagt cgacaatttt tatgtgtgcg gactcaatac tctactagat ggtatcaaga
     2221 tcccttggaa aaaaggtgag aatatctttt atctggagta tgaaaagtta ggtttgttag
     2281 ctggtgaata ttattttgac gttgcggtct ttgaagaaaa tgcaacggtt cctttggtat
     2341 acaagacaaa atacatgaac ttgtttgtca gcggttctta tatcggagaa ggcatcgtgg
     2401 tcttagacca taaatgggaa gaagggactc atagcaatga aatatgattt tgaaatggac
     2461 ctagatgaac aaagcagtgt gggaaaaatt gccgcacaga tcaaaccagg aagtaaagtt
     2521 ttagagtttg gtcccggaaa cggtcgatta acgaaacatt tgatcggagc aaaacaatgt
     2581 gaagtgagca ttgttgagct agataaggaa ttattcgatt ttgtcagcga atttgcccaa
     2641 gatggatttt atggagatat cgaaagtttt gaatgggcaa actattatgc tggacagaca
     2701 tttgattacg tcttgtttgc ggatgtttta gaacatttag tagatcctgg aaaaacattg
     2761 aagaaagtca gagaattttt gaatgaagaa ggagagatcc tgattacatt ccctaactta
     2821 gcacataatt ctgtcatgat cgatttgttc aataatcaat tgccttgggc ttcttatggt
     2881 ctcctagatg aaacccataa ttctttttat actcatgatg gtttccaaaa agtttttgaa
     2941 aaagccggat tattcattaa tattgaagat tacttgtact tggctgtagg agatacagaa
     3001 ttaaaatcaa cttatgaaga gttgccggaa gctgtacgct atgatttcaa aatgcgacca
     3061 tttggtgaag tctaccaata ctttttctca ttgatgaaac atccagtagt acaaacttcc
     3121 attgcacagc cacaaaactc taattatgta aaagtactag aagtaaccca gcaaacgaaa
     3181 caagatgaaa caatccaaca aataccattc aataatttca caggggaaaa tgagacacta
     3241 agtttccccg tgtcagaagc aacagaaaga atggtgtttc gcttcgcaca gcaacctagc
     3301 tttatcgaat tcagtgcaga agctgctgga gaaaaactca tatttatcga ttcaaatgct
     3361 gtagtcaaaa cagtaaatga ttgttatttg tttgatggga aagaaatacc agagttcgct
     3421 ctaacggata tttctgggaa agaagtgact atccattgtc actatcgctt tatcggggaa
     3481 ttgacaccaa caatgaaaga attgctggaa acaattcgtc ctatggctga agtcacaaag
     3541 cagctttctg agaaaaatga tgaattagaa agagaaaatc agcatcttct tgaggaaaat
     3601 acaagactag accatacgtt aaaaacaacc acaaatcgct attgcacatt gttggaaagt
     3661 gatgaatgga cgatcaaaca tagactagga cgacgtaaga aaaaaacatc gaaaaaaata
     3721 caagaaaaag agctttctat atgtattgat gagaaaatat gggatgcaga aacaaaaata
     3781 ttaaaaatca tcggatgggg aatcgctaat tcggatcgcc agccattgtc ctataagtta
     3841 tcagctgacc aatcgccatt tttcgaagca attcctgttt cacgagatga agtcaatcaa
     3901 gcagaacagc tcgcaaaagg aacagaagcg ggatttgaat tacgaatcct ttgcgagcaa
     3961 gaacgttcat tcttagtgga agcagtagct caaaatggtc aatcatggat cattgaaatg
     4021 taaataatga ttaacttata aagataagga gtaaacgaat cgtgcaaaaa aaagaaattg
     4081 ccgtatttat cgatcaaatc actcgtgaac gggccactgg cgatttgctt attgtgggct
     4141 gggcaattga tgaagtgacc aaagaaatcc ctacgataaa ggtagaaaaa gaaaatgtga
     4201 ttgctgaagc aacacatgtc gtaagattgg atatcaatca tttatataat cttgatgtta
     4261 agacccaaag cggttttaag atacgtttat ccgggaaaat gagaggaaaa gcgatcttag
     4321 attttcaaac ggcaaaacat caaaatggca tagctgtgaa actaaatggt aaataccctt
     4381 atgatgacgg cattgaatct tcatgggaaa gaaaaaaaag attactgaaa aaagggataa
     4441 attatgcaag gactcatggc gtaaaaaaaa caatccgccg ggtcaaactg gaattgaaac
     4501 caggatcgat cgactatgca gagtggatca gtcgtcacga gaaacctgat ctgaaagagc
     4561 aaaggaaaga agcacaaggg tttgcatacc gtccattgat ttccatactt atgcctgtct
     4621 ataacgttga aatcaaatgg ctcgaaaaat gcattgattc ggtattggat caaacgtatg
     4681 atcactggga actatgtatc agtgatgatg cttctactga tccagctatc cgaaagtgtc
     4741 tggaatccta tcaagcaaag gatgatagaa tcaaagttgt tttccgacaa gaaaatggac
     4801 atatcagtct ggcaactaat tctgcgttgg aaatggcaga aggagaattt atagcgctct
     4861 tagataatga tgatgaatta ccgccatttg cattatatga agtggcaaaa gtgctaaaca
     4921 tacatccaga actggatttg atctacagtg acgaagataa aatcgatgca gatggcaatc
     4981 gctttgatcc gcacttcaaa gcagactggt ctcccgatac attgatggga aataattata
     5041 tctctcactt aggcgtatat cgaacaagta ttgtgaaaaa gcttggcggt ttccgtaaag
     5101 gctacgaagg ctctcaagat tatgatttgg tccttcgtgt gacagaacaa atccctgcag
     5161 atcatattta ccatattgac cgagtcttgt atcattggcg gacgatccca ggatcaaccg
     5221 caagtaatgg tgaagccaaa agttatatct atgattctgg agtgaaagca ttgacagatg
     5281 ccctgtctag aaggaatatc aaaggaagcg tacgtcctgg cagaatttct ggattttatg
     5341 agattgctta tgacgtcttg caagaggatt tggtcagcgt gatcattcca acgaagaatg
     5401 gctatgaaga tctaaaaact tgtgtggatt cgattatcga aaaaacgtct tatccaaatt
     5461 atgaaatcat tattgcagat aatggaagta cagatcctaa aatgcaggaa ttatttgcag
     5521 aatacaagca tcagctgaaa gaccgattta tcgttgaatt aattgacatt ccattcaatt
     5581 attcacgtat caacaatctg gcagcagaaa aagcaaacgg caaatacttt ttattcttga
     5641 ataacgatac agaagtgatt gaacctgact ggatgacagc gatggtttct tatgcacagt
     5701 ttgatcgaat cggctgtgta ggagcaaaac tgttttatcc agatgataca acgcagcatg
     5761 caggagtatt attaggaatc ggtggtgttg caggacatgc attgaataac tatgatcgaa
     5821 cacattgtgg ttatttcggc cgattggtca ttgacgtcaa ttacttagca gtcactgcag
     5881 cttgtatgat ggtcaaggca gctgacttca atgctgtcaa tggatttgat gaaacattag
     5941 aagtagcatt caatgatgtt gacttgtgtt tgaaagttta tgaactgggt cgttacaatg
     6001 tctatgcaca tcaagcagaa ttgtatcatt tcgaatctaa atcaagaggt tatgaagata
     6061 caccagaaaa acaaagacgt tttgccgggg aaataaaaaa aatgcaagat aagtggcctt
     6121 cttatattgc tcatgaccca ttttataacg ataatctaac taaagaagga atcggcgatt
     6181 tttcattgag acctgattga ttaaaagaac tgtattttca tttttattga tgaaaataca
     6241 gtttttttag tatatgaaaa ttgtaacttg tgaccgaaga gatgatagga agaacaccca
     6301 ttcagtagga agaatgtgat acgatttttg tccaactctc tgttaggcag aaatatacat
     6361 ataaacatac aatagtcacc ttttctagtt tcttttaatt tattttacaa tttggttatt
     6421 cactaggttt ttaaactttg ctatggtaag atgatagagg cattatttta tggttaggag
     6481 tgtatatatg aataaaaatg gggagtggaa tgcggcacgt aggatattca ttatcatcat
     6541 ggatgtactt gtattgaatg tgtcgatcta tcttagcttt ttgctgaagt tccaaggaga
     6601 agtcccagca agaaacttag agacctttca gcattcagca gtttttattt ccattatctt
     6661 tattgcgttg aatattttgt taggagctta tgtattctat agccgagtga tcagtgatat
     6721 cgtcttcatt actgttattg gacaggtgct gatagcattg gggatcatgg tagttacttt
     6781 tgctggacgt tggtttgcat ttccgcgtgg cgtcatcgtg ctctcgttta tacttggcac
     6841 gatattactt agcgtttatc ggatagttgt ttataaacta tacttaagag tcagtcgtga
     6901 taagaaagtc cttatcgtag gtttggagaa agatgtcgca ccagctatcc aaaactttca
     6961 atctaagaaa aacaacaaac ataaagttga agcagtagtg atcgacaagt attacgaaaa
     7021 cacaaaaaaa ttgattgatg atatcgatat tgtctacttg gcttctaaaa tcccagaaga
     7081 cgaaaaaatc aatatttatc aattagtaac taaaaaagag aagaagctat ttttaaatac
     7141 aacttttgaa aatttaacga tgatcaatcc gaacatcatg aatttcgaag atgaaagtat
     7201 cattgaagct tccggtttca aaattcagcc ggaatatgaa ttattcaaac gtctttttga
     7261 tatcatcgtt tcgcttattt tactgattct tgcttcacct tttatgctaa tcacagcgat
     7321 tttggtaaaa acaacttcac ctggaccaat tatttataaa caagtacgta ttacgaaaaa
     7381 tcaaaaggaa ttttcaatct ataaatttcg ttccatgagt gtaacagctg aagcaaaatc
     7441 aggaccggtc tttgcaaaga gcaatgatgc acgtgtgaca ccagtaggca aatttatccg
     7501 tgccgtacgt tttgacgaat tacctcaaat ctttaatgta ttgaaaggcg atatgtcgat
     7561 cgtcggacca cgtccagaaa gaccattttt cgttgatcag ttcaatgaag aaaatcctta
     7621 ttattattta agacacaatg tacgtgcagg gattaccgga tatgcacaag tatacggaaa
     7681 atatgtatca gattataata gtaaactacg ttttgatttg ttgtatatca aaaagtattc
     7741 cttactatta gatttgaaga ttttacttca aacaatcaaa atcttatttg ataagatgtc
     7801 ctcacaagga ctagatgaag atgaaaatat ccgttcgtta gcagaaatcg acttttcaca
     7861 agagaaaatt tatcgctaga tgggaaaaat tttgagcaaa gaagtaggag agaacattgg
     7921 aaaataaaaa tataagtatg ctgctttctt tatatgataa agaaaaacca gagtatttaa
     7981 aggaagcatt aactagtatt tttgagcaaa cagtaatgcc aaaagaaatt gtattagttt
     8041 atgacggccc tataaatgaa aaacttcaag tagttgtcac ttatttccaa caaaaagatc
     8101 ctcacttgtt tacagttgta aaattagaaa aaaatcaagg attggggatt gctttagcaa
     8161 ttggattaga acatacgaaa aatcgcttag tagccagaat ggatacagat gatatcatg
//
LOCUS       DACEXQ010000116         8109 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.116, whole genome shotgun sequence.
ACCESSION   DACEXQ010000116 DACEXQ010000000
VERSION     DACEXQ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8109
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.116"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..1469
                     /locus_tag="IXH04_001781"
     CDS             <1..1469
                     /locus_tag="IXH04_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAQ1852382.1"
                     /translation="LTTEIQLLNEKVNYLTNKLFGRSKETLFEETNGQLNLFSDEEIS
                     VSVPEAAATIIPVKGHQRVVGTKADKIKHLPITEKEHLLPLEEQFCEHCGSQMKDIGR
                     TKVREEIRFHQAMLDCLTHYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVA
                     ETIRMKFGQKVPAYRQENYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQ
                     EEILHGDETSYRVIESAKTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFT
                     GYLHCDGYSGYNAVESVRLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVL
                     EKKWKDFSPEKRLSCRQQELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEP
                     IFEDGRLELTNNRAERNIKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPV
                     KYLEFLFDKIPNLPVLSAETLDQLLPWNKDVQQHFSRN"
     gene            complement(1641..2213)
                     /locus_tag="IXH04_001782"
     CDS             complement(1641..2213)
                     /locus_tag="IXH04_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1852383.1"
                     /translation="MTNKDQNKNYLEAIILELQNNSKRINSVELEQFSEKIVQAKHIF
                     LAGAGRSGIAMQAFTNRLMHLGFSVSLVGEISSPHSQPGDLLIICSGSGETGSLKNLA
                     AKANQSGVSIALVTMKKDSSIGQLADSVLVLPGITKDENQREEDTFSQPMGSSFEQLA
                     FLTFDSLVLSLMDKTGESSETMFARHADFE"
     gene            complement(2220..2882)
                     /gene="rpe"
                     /locus_tag="IXH04_001783"
     CDS             complement(2220..2882)
                     /gene="rpe"
                     /locus_tag="IXH04_001783"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAQ1852384.1"
                     /translation="MTIAPSLLNAPIAEVKELFEILKEKNVDYLHIDVMDGCFVPDMA
                     FGPSVIKDIRPLTDVVFDVHLMIDTPEKIVTRFVEAGADIVTFHIEATKHAMKVIQLV
                     KEAGAKVGITFNPATPVSFIEPVLSLVDQVLVMTINPGTENKHFIQETVVKIEQLDVI
                     RKQNDYTYDIEVDGKIDNQTIKVCSKAGADIFVSGGFIFNGKNTPQEQIDKLNQSLEE
                     VK"
     gene            complement(2898..3413)
                     /locus_tag="IXH04_001784"
     CDS             complement(2898..3413)
                     /locus_tag="IXH04_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HAQ1852385.1"
                     /translation="MRFHHDTLTIETVSGRPSYHDIRKELLDTVDTAGIENGMLVISS
                     PHTTCSLFFEETMHDTNFFGDDYLHVDINNVMETIIPKMTSENQYNSPGPKHIDFGTR
                     LSDPNYPAEKWVMLNTDAHLRSSIYGTNSMTLIVQEGKLLLGEMGRVYFVDWDQLRER
                     TRTINLMIMGE"
     gene            complement(3415..3699)
                     /locus_tag="IXH04_001785"
     CDS             complement(3415..3699)
                     /locus_tag="IXH04_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1852386.1"
                     /translation="MKKLLVICGAGHATSTIAVAKLSKWLEEEGLTDQVKIYQSKIAD
                     ELNKIDEYDAVVSTTIVPDVIKAKVTNGLGLLTGIGTDAIYADVKERLGV"
     gene            complement(3733..5061)
                     /locus_tag="IXH04_001786"
     CDS             complement(3733..5061)
                     /locus_tag="IXH04_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAQ1852387.1"
                     /translation="MDIVLNAFEWFIGLGSNVFLPLIIFVIGILFGLKPGKAFVSGVT
                     VGIGSIGLGLVLSLLSDGLGTAIQQMGEQYGGGLNILDIGVGVGGPLAYSTSLGILMI
                     PISLILNFIFVVLGWTKTLNVDIWNFWFAIFLGMITQSVTGNFWFGLIGAIVAVILQW
                     FLADRTQKTVSEFFGYPGISITHMMGLSGVLFAKPVNWVFDRIPGFNKIDADAETLTK
                     RFGVLGDTVVIGLIIGIVVGIMAGYDVAGIGNLGIMTAAVMKIMPKMVAMFMEGLMPI
                     AEAAKEFTNKKLNGRQVNIGMDAALTVGHPTIMSTSLLLVPISLLLALILPGNKVLPF
                     GDLAFFAFAICLMIPYFKGNIVRSLVGCSLYVVTTLYMATWLAPIITDVFTLASFDVG
                     TAGLVTMLLCGLWPVGLFVLLASYFGVIGIGGLGVVLVGASIYMNKVKKA"
     gene            complement(5075..5530)
                     /locus_tag="IXH04_001787"
     CDS             complement(5075..5530)
                     /locus_tag="IXH04_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1852388.1"
                     /translation="MYLDKELCLFQLEVKDQKELFQVMSEQLKNAGCVKDSFLEGITN
                     REQEFPTGLEVNQIGFAIPHTDSAHVNSSQICFASLKEPLVFSDMTDKSHEIPVRLVF
                     MLAMSQPHEQIDTLQNLVSLFQNEEKVNELLACTTKEAFIELVHSAGVY"
     gene            complement(5533..7455)
                     /locus_tag="IXH04_001788"
                     /pseudo
     CDS             complement(5533..7455)
                     /locus_tag="IXH04_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341578.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
     gene            complement(7475..>8109)
                     /locus_tag="IXH04_001789"
     CDS             complement(7475..>8109)
                     /locus_tag="IXH04_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HAQ1852389.1"
                     /translation="LSSNLAYTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQ
                     AKLLNQIAKVGYNSTKKSYFYGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEA
                     NLPNILGDKGYISHKIHEKLALKGITISVPPRKNMDKSEKLDHSLLGKQRKTVETVFS
                     SLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         2625 a         1539 c         1279 g         2666 t
ORIGIN      
        1 atcttacaac tgaaatccaa ctattgaatg aaaaggtcaa ttatttaacc aacaaattat
       61 ttggacgctc taaagagact ctctttgagg aaacgaatgg gcaattgaat ctttttagtg
      121 atgaagaaat ttctgtaagt gttccagaag cagctgccac cattattcca gtaaaaggac
      181 atcaacgagt tgtaggaacg aaagcagaca aaatcaaaca cttacctata acagagaaag
      241 aacatctcct tcctttggaa gaacaatttt gtgaacattg cggttcacag atgaaagata
      301 tcggacggac aaaagtacga gaagaaattc gtttccatca ggctatgttg gattgtctca
      361 cccattatca acatacctat tgttgtaaaa gctgcgaaaa agaaggactt tcttctttta
      421 aaaaagcgat cgttcctaaa cctttgattt ctaatagctt aggttccaat agtttagtgg
      481 cggaaacgat ccggatgaaa tttggtcaga aagttcctgc ctatcggcaa gagaactatt
      541 ggaaacaaac acatgggttg gatatttcga gagataatat caccaattgg catattaaag
      601 cagtgcaaaa cgcgttagac cctttaggag aacgattaag agtctatctt aatcaagaag
      661 agattctaca cggggatgaa acaagttatc gtgtgattga aagtgccaaa acagatacct
      721 attactggca gttttgtaca ggaaaagaca gtcagcatcc catcgtttat tatcaccacg
      781 acgaaagtcg tgctggagat gttccgaaaa cttttctcaa ggagtttaca gggtatttac
      841 actgtgacgg ctatagcggg tataatgccg tggaaagtgt acgacttgtc tattgtttcg
      901 cgcatgttcg gcggaaattt ttcgaagcaa ttccaaaagg aaagaaaaac acggatattc
      961 ccgcagctca agcagtcaaa caattggata aatggtttgt cctagaaaag aaatggaaag
     1021 acttttctcc tgagaagcga ttaagctgtc ggcaacaaga actgcgtcca ttatttattg
     1081 ctttttatga gtggatggca acgatagatc ctgttgcaaa atccaagttg gatgcagccg
     1141 ttcaatatgc gtgtaaacta cgaagtggtt ttgaacctat ttttgaagat ggtcgtttag
     1201 aactcacaaa taatcgtgcg gagcgaaata tcaaagagct ggtgatagga cggaaaaatt
     1261 ggttgcattc cacaagtctt gaaggagcac gaacttcagg aatcattctg agtgtatata
     1321 aaacagcaga acttaacgga ttgaatcctg taaagtatct agagtttcta tttgataaga
     1381 taccaaatct tcccgtactt tcagcagaaa ctttagatca actgttgcct tggaacaagg
     1441 atgtacaaca acatttttct cgtaactaaa aacgccaaac attcccgatt ctattctata
     1501 ggtcgggtta tttgacgtac aggtactgtt ctatggggcg cttacgaatt attaagttat
     1561 gtctaatcaa ataaaaaaac cattctcata tttgctgaat aattagcatt tagagaatgg
     1621 ttttttatct ttaaaatcta ctactcaaaa tctgcatgtc gagcaaacat cgtttctgag
     1681 ctttcacctg ttttatccat taaagataac actaaactat caaaggtgag aaatgctaat
     1741 tgttcaaaac ttgaacccat aggttgtgaa aaagtatctt cttctcgttg attttcatct
     1801 ttggtaatac ctggtaatac aagaacagaa tctgctaatt gaccaatcga cgagtctttt
     1861 ttcatggtaa ctaaagcaat gcttacacca ctttgatttg ctttggccgc taaatttttt
     1921 aaactacctg tttcacctga gccagaacag ataattaata aatctccagg ttgcgaatgt
     1981 ggtgaactaa tttcaccgac aaggctgact gaaaaaccta aatgcattaa gcgattcgta
     2041 aatgcttgca tggcaattcc agagcgtcct gcaccagcta gaaagatgtg tttagcttga
     2101 acaattttct cactaaattg ttctaactct acagaattaa tacgttttga gttattttgt
     2161 aattctaaga taatcgcttc taaatagttt ttattttggt ctttattggt catgatcttt
     2221 tacttcactt cctctaatga ttgatttagt ttatcaattt gttcttgcgg ggtatttttc
     2281 ccattgaaga tgaaaccgcc tgatacaaaa atatccgccc ctgctttaga acaaacttta
     2341 atcgtttgat tatcaatctt gccatctact tcaatatcat acgtataatc attttgtttg
     2401 cgtattacat ctaactgttc gattttaact actgtctctt gaataaaatg cttattctct
     2461 gttcctggat tgattgtcat tactaatact tgatcgacta aagataatac tggttcaata
     2521 aaactgactg gtgttgcagg attaaaagta atccctactt ttgctcctgc ttccttgact
     2581 aactgaatga ccttcattgc gtgtttggta gcttcaatgt ggaaggtaac aatatctgct
     2641 cctgcttcta caaatcttgt gacaattttt tctggtgtat caatcatcaa atgaacatca
     2701 aacacaacgt ctgttagtgg acgaatatct ttaataacac ttggtccaaa agccatatct
     2761 ggtacaaaac agccatccat tacatcaata tgcaaataat caacattctt ttcttttaag
     2821 atttcaaata actctttaac ttctgcgata ggtgcattta acaatgatgg agcaattgtc
     2881 attctctatt cccccgttta ttctcccata atcatgagat tgattgttct agttcgctct
     2941 cttagttgat cccaatcgac aaaatagaca cggcccattt ctcctaataa aagtttacct
     3001 tcttgaacaa ttaaagtcat tgagttggtt ccataaatag atgaacgtaa atgtgcatcg
     3061 gtatttagca tgacccattt ttctgcaggg taattgggat cagataatct tgttccaaaa
     3121 tcaatgtgtt ttggccccgg actgttgtat tgattttcac tcgtcatttt tggaataatc
     3181 gtttccatta cattgttgat gtctacatgt aaataatcat caccaaaaaa gtttgtgtca
     3241 tgcatcgttt cttcaaaaaa gagtgagcaa gttgtatgcg ggctcgaaat tactaacatt
     3301 ccattctcaa tacctgctgt atcaactgta tctaaaagtt cttttcgaat atcatgatac
     3361 gaaggacgcc ctgaaactgt ttcaatagtc aaagtgtcat gatgaaaacg catattaaac
     3421 acctaatctt tcttttacat ctgcataaat ggcatctgta ccaatacctg ttaataagcc
     3481 caagccattc gttactttag ctttaattac atcagggaca attgttgtac ttactacagc
     3541 atcatattca tcaattttat ttagctcatc agcaatttta gattgataaa ttttcacttg
     3601 atctgttaat ccttcttctt ccaaccattt tgataatttt gctactgcaa ttgtagaagt
     3661 agcatgacct gctccacaaa taactaataa ttttttcata ttgatctctt ctttcttatt
     3721 ttaattttat gattaagctt tcttcacttt gttcatgtag atagaagcac ctactaaaac
     3781 aactcctaat ccacctattc caattacacc aaagtaagat gctaataaaa caaacaaccc
     3841 tactggccat aatccacata acaacatagt tactaatcct gctgtcccca catcgaaact
     3901 tgctaatgta aacacatcag tgataattgg tgccaaccat gttgccatat ataatgttgt
     3961 aacaacatac aacgaacatc ctactaatga tctcacaata tttcctttaa aataaggaat
     4021 cattaaacag atggcgaaag caaagaatgc taaatctcca aaaggtaata ctttatttcc
     4081 tggaagaatt agtgctagca ataatgagat tggtacaagc aatagagaag ttgacataat
     4141 agttggatga cccacagtta acgctgcatc cattccaatg tttacttggc gaccatttaa
     4201 ctttttattc gtaaattctt tcgccgcttc tgcaattggc attaaacctt ccataaacat
     4261 tgctaccatt ttaggcataa tcttcatcac tgccgcagtc ataattccta agtttccaat
     4321 tcctgctacg tcatagcctg ccataatacc cactacaata ccgataatta ggccaataac
     4381 aactgtgtca cctaaaacac cgaaacgttt tgttaatgtt tctgcatctg catctatttt
     4441 attaaatcca ggaatacgat caaaaaccca atttactggt ttggcaaata gtactcctga
     4501 taatcccatc atatgtgtga tagagattcc tggatagccg aaaaattcgc taacagtttt
     4561 ttgtgtacga tctgctaaga accattgtaa aattactgcg acaatcgcgc caattaagcc
     4621 aaaccaaaag tttcccgtaa cagattgagt aatcatacct aggaaaattg cgaaccaaaa
     4681 gttccaaata tcaacattca atgtttttgt ccaacccaaa accacaaata tgaagttaag
     4741 aattaatgaa attggaatca ttaaaattcc caatgatgta gagtaagcca aaggtccacc
     4801 aacacctacg ccaatatcta ggatattcag gccaccaccg tattgttccc ccatttgttg
     4861 aattgctgtt cctaaaccat ctgataataa acttaaaact aatcctaaac caatagaacc
     4921 gataccaaca gtaacgccgg aaacaaatgc tttaccaggt tttaatccaa ataatatccc
     4981 gattacaaaa ataatcagtg gtaaaaagac gtttgaccct aatccaataa accattcaaa
     5041 ggcatttaat acaatatcca tctctctatt cctcctaata aactcctgca gaatgtacta
     5101 actcaataaa tgcttctttc gtggtacatg caagtaattc atttactttt tcttcatttt
     5161 gaaataggct aactaagttt tgtagtgtat caatttgttc atgaggttgt gacattgcca
     5221 acataaagac taaacgtaca ggtatttcat gagatttatc tgtcatatct gaaaatacca
     5281 gtggttcctt taatgaggca aaacagattt gagagctatt tacatgtgcg gaatctgtat
     5341 gtgggatagc aaacccaatt tgatttactt ctaatccagt tggaaattct tgttcacggt
     5401 tggtgatacc ttctaagaaa ctatctttca cgcaaccagc gttttttaat tgttcagaca
     5461 ttacttgaaa caattctttt tgatctttca cttctaattg aaataaacat aattctttat
     5521 ctagatacat ttttattctc ctttttgttg aaaaattctt agtaattttt cgctatcatg
     5581 aacttgtaac aagcgatcga ccatttcttt ttgcccaata aattggtaaa gttcttcata
     5641 tactcctcta atctcagata catcttcttc agctaaacta accataatga ctaatttgac
     5701 ttttcgatcg ccccaattaa tcgcattttt taatgctaca ataaaaatgt tggattcaat
     5761 gaccattgat ggattcgcat gcggtaaagc aacaccactt tctaaattag tattccccat
     5821 tttctctcta gagaagatag aagcacgaaa ctcttcagta actaatgaat tggcttctag
     5881 ttcggaaatc atcaaatcca aacattcttc tttgctttcc acctgtactc ctgtttttac
     5941 taaagatgat tgaacaaatt tagatatggt gggaacttta accgctttag gaatcagttc
     6001 attttgaata aatcgcgaag gttcttctat taaatattta gtataagctt ccataatatt
     6061 catataatct gttttactta ccaatggaga tacccttact aagggtatac cacctgcatc
     6121 aaaggaaaga gaggaaataa tcaaatcaac ttccccaatt tcagctaaat gaaacttatc
     6181 taaagttgat atttctacat tatcttttgc tggtaaaaac cgtcgaactt tattgtaaat
     6241 taattgcgca gaagaaatac cgtattggca aataatgatg atattatttg ctttggactg
     6301 atcttcaata gcaatctgaa aatgaatcaa aattaaggaa acttcatcgt ccgttaaagt
     6361 cacatcaaat tcttcttcaa ggatggacat ggcgtaccat actatgctaa agagttctga
     6421 atattgagat ttgatatttt ctaataacgg attttgaatt ttaatacctt tttttaaacg
     6481 aaggaccatt gctggcacat gatacaataa ggattgatat aatcgatcat tttgagtcaa
     6541 atcaattttt tcaatctcac tcattctttc aatcaattta tgaacgctca tagcgtactc
     6601 ttcattgctg gtttttaaat tatttgtcac acgatgtgca aatagttgac gacatagata
     6661 ttctttatct gcgtcataaa atggaagatt caattttgcc ttcaattctt caattaaacc
     6721 atttgccaca atgtacgttt ccatatagcg aatgctattg aagaggaatt cttcttcaac
     6781 agttaaatga tgacctttca gtaaacggtt acattggata actaaagtag aaatcaatga
     6841 ccgaacatag taatcagaaa ccttttcagt cagttcactg taatcattga atagcaaatc
     6901 atgaatgaca ctaataactt ttgaatcaaa caacatattt aaaacttgaa aaaatgtttt
     6961 tgattccatg tcattcgcat aatagaatac aagttgcttg attgctcgtt gaattgcttg
     7021 ttcattacca gtaaaactaa ttccttctgc agatgactgc aacttaactt ctccactgcc
     7081 aataatttta ttgataattc tcaggtcatt gtataacgat gttttactaa ccataaaact
     7141 ttgagacaaa tgagttaaag aaacaggctt atcaccaaac aaaacttctt taatgatctc
     7201 gacacgtctt ttttctggag aataaatatc tttttcaaga taattttcta ccgatgaaat
     7261 caaatgttca acgtttttcc tatcacctat gagtttgata cccaaagaag gtaatcgctc
     7321 aattgttact tggtaattaa ctacataatc ttccaacaat tcaatatctt gataaatggt
     7381 cttggtcgac actgccaatt tatcactaaa aaaagaagcg ggtttaaagt cttcttgtcg
     7441 acttaataat tttaacccgt ggtgctagtt aattttaata tcctaaagaa tatctcaaag
     7501 ttccttcaaa acgtcgttgt gctcgactta atagaacact gtaagccagt aatatgcttt
     7561 caaatctact ctctagccct ttaacagaac gtgagttgaa attttgacaa cctaattttt
     7621 ctaaggaaga aaaaacagtc tcaactgttt ttctttgttt tccaagtaag ctgtggtcta
     7681 gtttttctga tttatccatg tttttacgtg gcggaacgga tatagtaatg cccttaaggg
     7741 caagcttttc atggatttta tggctaatat atcctttgtc ccctaggata ttgggaagat
     7801 tcgcttcttc cgataaagtc tctaacactt tcacgtcatg gacacctgga ttcgtgattg
     7861 agtaagtaat cggaaagcca gtcttagtaa cgatcatatg aattttaaga ccataaaaat
     7921 aagatttttt tgttgagtta tagccaactt tggctatttg attcaatagt tttgcttgtc
     7981 tatttctaac tggtttacat aatggactag gaaaactatc tataattccg actagttcac
     8041 ctttggtgag cttcttgatg aaaaagtatc gaataatctt gatagtgtaa gctaagtttg
     8101 aacttaagc
//
LOCUS       DACEXQ010000117         8081 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.117, whole genome shotgun sequence.
ACCESSION   DACEXQ010000117 DACEXQ010000000
VERSION     DACEXQ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8081
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.117"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(784..1191)
                     /gene="arsC"
                     /locus_tag="IXH04_001790"
     CDS             complement(784..1191)
                     /gene="arsC"
                     /locus_tag="IXH04_001790"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (thioredoxin)"
                     /protein_id="HAQ1852390.1"
                     /translation="MRKIYFLCTGNSCRSQIAEGYGKAILPTDEFEIKSAGIEQHGLN
                     PRAVQVMAEEGIDISTQTSDLIDMDYFNQADLIITLCGDAKDKCPVIPKGIKHVHWDL
                     EDPAKATGTEEEIINKFREIRDDIKSRVANLLD"
     gene            1549..1908
                     /locus_tag="IXH04_001791"
     CDS             1549..1908
                     /locus_tag="IXH04_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ1852391.1"
                     /translation="MKNEICEISCVHEDKVLLGKEKLQTEDISSLKSVFKILADENRL
                     KIIYALSYEDELCVCDIAATIEATVATTSHHLLSLKKNGIVVSRKVGKLVYYSIKNDK
                     FVQLLNSKLYLEDEVLV"
     gene            1905..4022
                     /gene="cadA"
                     /locus_tag="IXH04_001792"
     CDS             1905..4022
                     /gene="cadA"
                     /locus_tag="IXH04_001792"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ1852392.1"
                     /translation="MSEKKQVYRVEGLSCTNCAAKFEKNVSQIPKVTDAKVNFGAGKI
                     SIEGEATIAEIEAAGAFENLKVQSEHDTEPRIETKEPFVKRNWNLLVSLFLIILALGS
                     QVVNGEDALLTEGLFILAIIIGGFSLFKEGFSDLLKLNFSMESLMTIAIIGAAIIGEW
                     TEGSIVVILFAISEALERFSMDKARQSIRSLMDIAPKEALIRRNNVEQMINVSKIEVG
                     DIMIIKPGQKIAMDGQVIKGHSSVNQAAITGESVPIEKNINDDIFAGTINEEGALEVK
                     VTKHVNDTTIAKIIHLVEEAQGERAPAQAFVDKFAKYYTPTIMVIAALIVIVPPLFFN
                     GDWNTWLYQGLSLLVVGCPCSLVISTPVSIVSAIGNSAKNGVLVKGGIYLEEIGGLKA
                     IAFDKTGTLTKGTPTVTDFTTVDSKDEEKYFSIITALESYSQHPLASAILKEADNRAI
                     SYKSVVVDEFTSITGKGIQGNIEGITYLVGSPKLFESILTDNSKIIENYQRLQQQGKT
                     AMLLGTDKQILAVIAVADELRESSKAVIEKLHDLGIEHTIMLTGDNATTAQSIGKQTG
                     VTEIKGDLMPQDKLDYIKSLKETYGKVAMVGDGINDAPALAASTVGIAMGGAGTDTAL
                     ETADVALMGDDLQKLPFIVKLSRQTLRIIKQNITFSLGIKLLALLLVVPGWLTLWIAI
                     LADMGATILVTLNGLRLMKVKSK"
     gene            complement(4243..5055)
                     /locus_tag="IXH04_001793"
     CDS             complement(4243..5055)
                     /locus_tag="IXH04_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1852393.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGTQENQYILEMLQKKAGKLDPKIEGLIVDELKQ
                     AGLVSEKEMDRMIEFFIINLIPPTEQDQFTYQCIDEKKQLYLGSTKFGKEAQLYLHHD
                     NQNHILHILPFTFSNFSVDRLLVYLANQESYSYFGKHLPKELVEKAILLYSINQLTVD
                     VRIAPLVYSKETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNVAYPSSK"
     gene            5217..6473
                     /locus_tag="IXH04_001794"
     CDS             5217..6473
                     /locus_tag="IXH04_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ1852394.1"
                     /translation="MNQVNQHDLGESIRVSREERGWTQRYLAEKVGISRSLLSKVEKG
                     TRQLSAEKLNLILDSLQEEIVPVNRVLIDYLTIHFFSNQHLKLIEAIIGMPIERFEEL
                     DYAPKGYIGQYVWNQVITIRYSIDDTVKGTVMEFSGQGCKHLAMRLKTAKSNWQEFFR
                     KVLDYQGNFTRIDFTLDDFVGSLSIPELKRKVTLGHVWTTFQVSESHGGTDIINNESN
                     GETLYLGSKKSQCRFCFYQKDYEQRKRRGISLEEAEVKNRFELRYRKEKAQSLAKIIS
                     KTHDLTKLFFELLNGAICFYDRAPNDPGAKVDAKWAAFIGNHGATTISLETIPQSFEK
                     SMNWLIHSVSPTLAFIQEVDNHFDSNLINEIISCGELSSRQQKILENLIAEPDYYQEE
                     VEFYIQCLQNMKAAKIHKKSKAQLTH"
     gene            6563..6760
                     /locus_tag="IXH04_001795"
     CDS             6563..6760
                     /locus_tag="IXH04_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002403995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ1852395.1"
                     /translation="MKNTITTYELPYLLTREQASHFLGIDPKSFDKFVRANDELERFM
                     IGRQERYTVTSLINFIKTHSI"
     gene            6866..7915
                     /locus_tag="IXH04_001796"
     CDS             6866..7915
                     /locus_tag="IXH04_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1852396.1"
                     /translation="MPSIIKRGNSYRAQISLYNHGQHKKLTKSFSSKKEATRWALENE
                     LEKGNGKQLAERTTTFADFFENWIHIIKKNDVKETTFQNYQRTSQVVKKLFGDIQLKD
                     LNDIVVQRKIDKYAETHSRKTTHEVLLKIKTALRDAYARGYLATDFANLVKTRGKVLD
                     KRNRVLSITELKKLRTYVINHRENEFNILVLLALETGMRRGELLGIRPEDLFEYGIHV
                     RRSLSPTSEDTSLKTKNSRRDISINQEVYDILKSVPIKDNGYFFDPDGFQQSAKLARL
                     LKKLDIPKTTFHGLRDTHASFLFSQDIDIAYVSKRLGHINIQTTQNYYLELMPEKKHQ
                     QDADALNLLNSLSNL"
BASE COUNT         2695 a         1384 c         1499 g         2503 t
ORIGIN      
        1 aagcggataa ataatattgt ttgatgatac tttatctttt tcccgtttgt ttatagatcg
       61 aatgatatta tagaccaaga atcctaccta ttaacatagc taaaaacaca aaaaaggcac
      121 tttccaaatt gtggaaagtg ccagaaaagt taggattaag gtaactgcgc tcagcattgc
      181 ttcgcccagt acgattctcg taactcgtga actcgaacga taatcgcaat taacgttttg
      241 agatgtgact cgtacttatt acaagtactt ctatcaatag aaatccaatt atatcaacat
      301 tacaaacatc taaaaaacag tttgatatgg ataaaatagg attaatattg atattacttg
      361 aaccaatttg aaccaaagat gaatggtagt tgaatacaat tttcttacta gtttatcaat
      421 cttttttaga gttattcctc ccctcttatt tatttgagac cgtattgatg gctggatagc
      481 tgtctaatag gggtttcaag gccagaatta gctaagctac tagattctcg aataattgta
      541 taagccggtc acacctactt aaatgtgatt gagaaggtca aatatagggt caaaaaatac
      601 tcgtcagtat gtcagataga taaatccaat tttacgccaa aagcagatcc ttttctctta
      661 aaatagtaag agccatcgaa gatttatgtt tcacttcgca cggcttttac tttaattttc
      721 caatttatca aaaggacatt ttacgtcttt ttatctttta ccgagataga tgacagttgc
      781 tacttaatct agcaaattcg ctacacgact ttttatatca tctctaattt ctctgaattt
      841 attaatgatt tcttcttctg ttccagttgc tttggctgga tcttccaaat cccaatgaac
      901 atgcttaatc ccttttggaa ttactggaca tttatctttg gcatcgccac atagcgtaat
      961 aattaggtct gcttgattaa aataatccat atcgatcaaa tcagatgttt gcgtagagat
     1021 atctatccct tcctcagcca ttacttgaac ggctctagga tttaacccat gttgttcaat
     1081 gccggcgctt ttaatttcaa attcatctgt tggcaaaatc gctttcccat agccttctgc
     1141 aatttgacta cgacaagaat tgcctgtaca taaaaagtaa atttttctca ttattatttc
     1201 ctccgatatc agacaattct gatggatttt gaatgaacca atttttttgt gcgattagat
     1261 attttaacta acagtaacat gactggcact tcaactaaga tgccaactgc cggcgcttat
     1321 gtaaaaatat agataatcat ctatgttttg tcaaatgctt ctgtctttca gttcaaatta
     1381 attagtttat tatttgcatt cgattcaatc taatttgaaa atcactactt atatagatgc
     1441 atccatacaa tctatataac tcctcacata ttcaaattaa aatttgatta ttctattaat
     1501 ctatgataca ctacattcaa atatccatta gaataaagga ggagctgtat gaaaaacgaa
     1561 atttgtgaaa tctcatgtgt tcatgaagat aaagttttac taggaaaaga aaagttacaa
     1621 actgaggata tttcgagctt aaaaagtgtc ttcaaaattt tagctgatga gaatcgctta
     1681 aaaattatct acgccctaag ttatgaggat gaattatgtg tatgtgatat cgcagcaact
     1741 atcgaggcaa ccgtggcaac aacctcacac catttattat cattgaaaaa aaatggaatt
     1801 gttgtgagtc ggaaagtagg gaaattggtt tattactcta tcaagaatga taaatttgtc
     1861 caactactaa attccaaact gtatttagaa gatgaggtgc tagtatgagt gaaaaaaaac
     1921 aagtctatcg ggttgaagga ttgagctgta cgaattgtgc ggcaaaattc gaaaaaaatg
     1981 tttctcaaat accaaaagtt accgatgcaa aagtaaattt cggtgccgga aaaatctcta
     2041 tcgaaggcga agcgactatt gcggaaattg aagctgcagg agcttttgag aatcttaaag
     2101 ttcagtccga acatgataca gaacctcgca ttgaaacaaa agagcctttt gtgaaaagaa
     2161 actggaacct attggtttca ctgtttttaa ttattttggc attaggatct caagtagtta
     2221 acggtgaaga tgcgctatta acagaaggat tatttatact agcgattatt atcggcggat
     2281 ttagtttgtt taaagaagga ttttcagacc tattaaaatt gaatttctca atggaatctc
     2341 ttatgacaat tgcaatcatt ggtgctgcaa ttattggcga gtggactgag ggctcaatcg
     2401 ttgttatctt atttgcaatt agcgaagctt tagaacggtt ttcaatggat aaagcaagac
     2461 aatcaatacg ttccctaatg gatattgctc cgaaagaagc attaattaga agaaataatg
     2521 ttgaacaaat gattaatgtt tcaaaaatcg aagtaggcga tattatgatc attaagcctg
     2581 gacaaaaaat tgctatggat ggtcaagtca ttaaaggcca ttcctcagta aatcaggctg
     2641 cgattaccgg tgaatctgtc cctattgaaa aaaatattaa tgatgatatc tttgcaggaa
     2701 cgattaatga agaaggtgca cttgaagtca aagttacaaa gcatgtgaac gacacgacaa
     2761 ttgccaaaat tattcaccta gttgaagaag cacaaggtga acgagcacct gctcaagcat
     2821 ttgtcgataa atttgcgaaa tattatacac ccaccattat ggtgattgcc gcattaatcg
     2881 tcatcgttcc cccattattt tttaacggag attggaatac ttggttgtat caagggctct
     2941 ctttattagt tgttggctgt ccttgttctt tagtaatctc aacacctgtg tctatcgttt
     3001 cagcaattgg aaactctgct aaaaatggtg ttttggtaaa aggcggtatt tatcttgaag
     3061 aaattggtgg cctcaaggcg attgcctttg acaaaacagg aacattgact aaaggaacac
     3121 caacggtaac agatttcact acagttgatt caaaagacga agagaaatat ttctcaatta
     3181 ttacagcatt agaatcatat tctcaacacc ctctcgcgtc agcaatctta aaagaagctg
     3241 ataatagagc gatttcttat aaatcagtag ttgttgacga gtttacttca attactggaa
     3301 aaggaataca aggaaacatt gaggggatta cctatcttgt agggagtcca aagctttttg
     3361 aatctatctt aacagataac tcaaagatta ttgaaaatta ccaaagactt cagcaacaag
     3421 gaaaaacagc aatgctctta ggaacagata aacaaatttt agcagtcatt gcagtagctg
     3481 atgagttaag agaatcaagt aaagcagtca ttgaaaaatt acatgattta ggaatcgagc
     3541 atacgataat gctgactggt gataatgcta caactgctca gtcaatcgga aaacaaacag
     3601 gtgtaactga aataaaaggc gatttgatgc ctcaagataa attagattat attaaatcac
     3661 ttaaggaaac ctatggaaaa gtcgccatgg ttggtgatgg tattaatgac gcaccagcat
     3721 tagctgcctc aacagtcggt atcgcaatgg gtggcgctgg aacagataca gctttagaaa
     3781 cagctgatgt agctttaatg ggtgatgact tacagaaact tccctttata gttaaattaa
     3841 gtcgacaaac attgagaatc attaagcaaa atattacctt ctcattggga atcaaactat
     3901 tagcattatt acttgtagtt ccaggctggt taacgttatg gattgctatc ttagctgata
     3961 tgggagcaac tatattagtt accttgaacg gcttacgact gatgaaagtt aaatctaaat
     4021 agacaagtag tagcataaaa agaggaaaga ctagcgacgg cagcgccgta taagctagtc
     4081 tttcctcttt ttaatgctgt tgcaggacgg cgtagccgga ctgcatgggc aagcccctct
     4141 ttctaacagg gggctaacaa agaacaggaa actgtgacaa ctacccattt gggagtgtcc
     4201 tagcagtgtt ttctttgtca catgcttatc aatcttgtca cctcattttg atgatggata
     4261 cgctacgttt aacagacgtt ctttctcgac ttccatcacg aatggtagat aaagatattg
     4321 ttgttctagt agatattgat tgtcctctgt gtactgataa gaagctgttt cttttgaata
     4381 gaccaacggg gcaattcgca catcgacggt caattgattg atcgagtata gaagaatggc
     4441 tttttcaact agttccttgg gcaaatgttt cccaaaatag gaataggatt cttgatttgc
     4501 taaatagacg agtaagcgat ccacactaaa gttggaaaag gtaaagggca gaatgtgcag
     4561 aatgtgattt tgattatcat ggtgtaaata gagttgagct tctttaccaa atttagttga
     4621 accaaggtaa agctgctttt tctcatcgat acattgataa gtaaattggt cttgttccgt
     4681 tggtggaatg aggtttataa taaaaaattc aatcatccga tccatttctt tttcactaac
     4741 aagaccggct tgtttcagtt cgtctactat tagcccttca atcttaggat caagcttacc
     4801 tgccttcttt tgaagcattt ctaaaatgta ttggttttct tgagtaccat acaaaagata
     4861 ttcgttggtg acatggccta tttctgcaat ttggtgaagg gtttcggttt ttggaaggtt
     4921 aatcccacgt tcccagttat tgactgtact tcgtggtaaa tcaccaatta gtttgccaaa
     4981 tttctccata ctcaacttta actcttgacg aatctgccga attcgattgc ccactgcttg
     5041 cttgtctatc tccatgaaaa ctcctcctca tgataagttc ttaaattcag tatacgcttg
     5101 tacttaaaaa aagtcaaaat gtattgactt aaaataaata caggcgtaca atcgatttgt
     5161 acttaaaata agtctgatgg ttctttgaca atttaatagc agaaggaggt ttttctatga
     5221 accaagtcaa tcaacatgat ctcggggaat cgattcgagt ttctcgagaa gaaagggggt
     5281 ggacccagcg atatttagca gaaaaagtgg ggatttcgag atcgttatta tcgaaagtag
     5341 aaaaaggaac gagacaatta agtgcagaaa aattgaatct tatactggat agtttgcaag
     5401 aagagatcgt tccagttaat cgtgttttga ttgattatct aactattcac tttttctcga
     5461 atcaacattt aaagttgatt gaagcaatta ttggcatgcc tattgaacgc tttgaggaac
     5521 tggattatgc gccaaagggc tatattgggc aatatgtttg gaatcaagta atcacgattc
     5581 ggtattccat tgacgatacg gtaaaaggaa ccgtaatgga gttttcaggt caaggctgta
     5641 aacatcttgc catgcgtttg aagaccgcaa aatcaaactg gcaagagttc ttccgtaagg
     5701 tattagatta tcagggaaac tttacacgga ttgattttac tttagatgat tttgtgggga
     5761 gtctcagcat tccagaactg aagcgtaagg taacactagg ccatgtgtgg accacttttc
     5821 aagtgagtga atctcatggc ggtacggata tcataaacaa tgaatcaaat ggtgagacct
     5881 tgtatttagg ctcaaagaag agtcagtgtc gcttttgctt ttatcagaag gattatgaac
     5941 aacgaaagcg acgaggaatt tctttagaag aagcagaagt aaaaaatcgc tttgaactac
     6001 ggtatcgaaa agagaaagcg caaagcttgg caaagattat ttcaaaaacc catgatttaa
     6061 ccaaactatt ctttgagtta cttaatggag caatttgttt ttatgaccgt gctccaaatg
     6121 atccaggtgc aaaagtcgat gccaaatggg cagcctttat tggcaatcat ggcgcaacca
     6181 ccatttcttt agaaacaatt cctcaaagct ttgagaaaag tatgaattgg ttaattcaca
     6241 gcgtttctcc caccctcgca tttattcaag aggtagataa tcattttgat tcaaatttga
     6301 ttaacgagat tattagctgt ggggaactta gttcaaggca gcaaaaaata ttagaaaatt
     6361 tgattgctga gcctgattat tatcaggaag aagtagaatt ttatatccag tgtcttcaaa
     6421 atatgaaggc agcaaaaata cacaaaaaaa gcaaagcaca gcttacacat taaaatgtat
     6481 aaacgtactc cgccctagac aagttgagta tagcacattt ccttgtgtca gccaaaaaac
     6541 atttttggaa aggaaataaa ctatgaaaaa tacaataact acttatgaat tgccctatct
     6601 acttactaga gaacaagctt ctcatttttt agggatcgat ccaaaatctt ttgataaatt
     6661 cgttcgcgca aacgatgagt tagagcgttt tatgatagga agacaagaac gatacactgt
     6721 cacttccctc attaatttta ttaagacgca ctctatctag ctaaaatatc gtaggatggt
     6781 tgattagatg cttttcgcca tgtagaatga cggtgtattc aactagcatt tatcaaccat
     6841 ctttcagtga aaggatttgt tataaatgcc tagtataatt aaacgtggta actcatatag
     6901 agctcagatc tcactttaca atcatggtca acataagaaa ttgacaaaat catttagctc
     6961 taaaaaggaa gcaactcgtt gggccttaga aaatgaattg gagaaaggaa atggaaaaca
     7021 attagcagag cgtacaacaa catttgctga cttctttgaa aactggattc atatcataaa
     7081 gaaaaacgat gtaaaagaaa cgacttttca aaattatcaa aggacttctc aagtggtgaa
     7141 gaaattattt ggcgatatac agttaaagga tttgaacgat attgtagtac aacgaaaaat
     7201 agataagtat gcagaaactc attctcgtaa aaccactcat gaagtactat tgaaaatcaa
     7261 aacggcatta cgtgatgctt atgctcgtgg ctatcttgct accgattttg caaatttagt
     7321 caaaacacga gggaaagttt tagataagag aaaccgtgta ctttctatta ccgaattaaa
     7381 gaaactgcgt acttatgtca ttaatcatcg agaaaatgag tttaacattc ttgtactcct
     7441 tgccttagaa actggcatga gacgcggaga gcttttaggt atacgtcccg aagacctctt
     7501 tgaatatggt atccatgtca gacgttccct tagtccaact tctgaagata cctcgttaaa
     7561 aacaaaaaat tcaagacgag atatttccat taatcaagaa gtctacgata ttctaaaatc
     7621 tgtcccgatt aaagataatg gatatttttt tgatcctgac ggctttcaac aatctgctaa
     7681 gctcgccaga ttactgaaaa aactagatat tccaaaaact accttccacg gcctaagaga
     7741 tactcatgct tcttttctat tttcacaaga tattgatatt gcctatgtat caaaacgctt
     7801 aggtcatatc aatattcaaa caacgcagaa ctattatctt gaattgatgc cagaaaaaaa
     7861 gcaccagcaa gatgctgatg ctttaaacct cttaaattct ttgtcaaatt tataatttga
     7921 accaacttga accaaaaaat tcttgtaaac gttgatacaa taaggaattg attaacgttt
     7981 tgagaactgt gaagctttac gggctttctt aagacctggt taaacaaaac tctattttat
     8041 tgcattttac aggaatgtat ttcatgcgaa attaataagt c
//
LOCUS       DACEXQ010000118         8056 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.118, whole genome shotgun sequence.
ACCESSION   DACEXQ010000118 DACEXQ010000000
VERSION     DACEXQ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.118"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..968)
                     /locus_tag="IXH04_001797"
     CDS             complement(<1..968)
                     /locus_tag="IXH04_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1852397.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYY"
     gene            complement(965..2860)
                     /locus_tag="IXH04_001798"
     CDS             complement(965..2860)
                     /locus_tag="IXH04_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1852398.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(2918..4693)
                     /locus_tag="IXH04_001799"
     CDS             complement(2918..4693)
                     /locus_tag="IXH04_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1852399.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYLVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESSEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(4743..6239)
                     /locus_tag="IXH04_001800"
     CDS             complement(4743..6239)
                     /locus_tag="IXH04_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1852400.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     EIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRIWKEEDCAPVMLDGDEQYLQANTVDYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(6433..8055)
                     /locus_tag="IXH04_001801"
     CDS             complement(6433..8055)
                     /locus_tag="IXH04_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ1852401.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
BASE COUNT         2413 a         1678 c         1288 g         2677 t
ORIGIN      
        1 atgtagtagc taaaagaaac aaaatcaaca gggtaggctg ctaagatttc ttcatcacct
       61 acttctattt ttaagttgac acctttttct tcaaacattc gttgcgtata cgctggataa
      121 tatcctcttg cttgaacatc actaaagaac aaagaagttc tttgctgctc aattgttttt
      181 tgaatatcat ccggatcaca agaatacgga acaagaagct gatagctgat catacaaccg
      241 acttgtgaac ctttgataat ttcatgacaa gcttttgtag ctaaagcact tgctataaac
      301 tgatggtgtg caccttggta tttcgcttgt tccaaatggg gatggttttc ttcaattact
      361 cctacgctaa caaaagggtg atgttttaca cagttgatct cattgaaagt catccaatat
      421 tttactttat ttttatagcg tttgaatact gtttcagcga aacgaacgta aaactgaata
      481 agctttcgat taggccatcc accatattct gtaaccaaat ataacggcat ttcataatgg
      541 gaaagagaaa cgactggctc aatcccatat tttagacatt cgtcaaacac ttgatcataa
      601 aaaaataatc ccgcttcatt gggtgtttct tcatcaccat gaggaaaaat tcttgtccat
      661 gcgatagaca tccgatagca tttaaaaccc atttcagcaa ataaacggat gtcttcttta
      721 aagtgatgat agaaatcatt tccgtgtctt ttaggataat aatacttgct gttgggatcc
      781 tgcgccgctt ttacttgctg atgcgtcatc atatgccatt gatctgtcca tttttcttta
      841 ggtaaatcgc tgtcgaatgt atacacatca gacacagata atcctttttc atctacatcc
      901 catgcacctt cacattgatt ggccgctgtc gctccgcccc ataaaaatcc ttctggaaat
      961 tttttcatat cgatccccct aaacttgcta gttttcttgt ttgattattt ttcttatgac
     1021 tagtaatgat atttttgaat ctactactat ttgtaacaat aaccattgtg gtcggatcat
     1081 atccctcttt gattaattct tgcaaatcta cttccatcaa taaatcgcct ttacgtactt
     1141 tttttccatc agatactgca ctttgaaagt atttgccatt taaattcact gtatcaatac
     1201 cgatatggat cagtatctct gctccttctt cactgactac cccaattgca tgtttcgttg
     1261 gaaaaactgt tgaaacttcc ccgtttacag gactgtagat ttttccgtcc gttggaatga
     1321 tcgcaaaccc ttctcctaaa gatccactag caaatgttgg atcgtttact tcagataaat
     1381 caattatttc acctgctgca ggagtactta acactatatc ctttgaggat tcttcttcga
     1441 tttcatcctt gtagccgaaa agaaaagtta gcatacagga actgatcatc gctaagaaat
     1501 aactagcaac tagccaaaca aatccttcac caatataaat cgggaaagac aaaatacttc
     1561 ttgtagcaac tgccaaagca ccggctcctg cggcaccagc tactccacca aatatagctc
     1621 cacatataca agcaatgtaa aatggtcgtt tatatttaag agtcacacca taaacagcag
     1681 gttcagtgat tccaaataat gctgtgataa atgcagataa agataaactt tttactttct
     1741 tatttttcgt tttcaaaaat accccaagta ctgctcctgc ttgcgcagct acggcagtca
     1801 tacatgctgg acctagcgta tcacgaccgt agaaagaaag attgttggtc attactggta
     1861 caagtcccca atgtaaccca aaaatcacta acacttgcca aaatccgccg ataacagcac
     1921 cagataaaac agggctaaag ttataaacag ctaaatatcc agctgcaatc agctgacttg
     1981 cataatctgc aattggacct acaactaata aagtaattgg gaccatcacg atgatacaaa
     2041 tgaatggtgt caataaattt cgaatacttt cgtgcaagct tttgcgaatc ttaggctcaa
     2101 tataagaaag aacccataca gctaaaattg ctggaataac cgtacttgta tatcttgcta
     2161 gaatcacggg catccctaga aatcgcaagg ttatgctatc actatatgca ctgacaatcg
     2221 taggataaac gagagctgct gcaataacca tagcgatgaa acgatctgta ttgaactttt
     2281 ttgcagcagt atatgccaaa aatataggca agaaatagaa aaatgcatca gctgctgcat
     2341 tcaaaatgat ataagttcct gattcagtag ttaataaatt caaactcgta cataaggcga
     2401 gtactccttt gatcaaacca cttcctgcca agacgcctaa agtcggggca aaaacagcgg
     2461 cgatcatgtc tataaaagag tttaaaatgc cttttttctc agttcctctc tttccactag
     2521 atgcatcatc ttctagtttc atttgtttgg ataatacgtc atacacttca tttactgcat
     2581 ttcctactac gacttgaaac attcctccag cttcgactac agtgatcaca ccttctagtt
     2641 gctctacctt ctccttatct gccttcttat tatcttttag ggtaaagcgt aaccttgttg
     2701 cacaatggta aacattgctg acattttctt cgcctccgac aaatcggaca atatctttcg
     2761 ctaattgtga atagtccatt tttacttccc ccttgttaat taatgccgta gcaatccttc
     2821 ttacaagata agtatagttc ttagaaaacg gtttctccat tcaattagta ccccttctaa
     2881 tagcgaacga aactgtacaa tagtgtgtat ggtttactta gccgagcaaa tcactcactg
     2941 cttgaaagaa aacaagtata tcgtttctct tatagtcaaa ggagacaaaa aaaacacgct
     3001 tgatcttcct ttgctgatgt ataagtggta tctcaagctg atactctgtg atttttgttt
     3061 ctacttggct tttgataaga cagcacaaca aagtctcggc atttaacgga agcataacta
     3121 ggtcagaact aggagagatt agttgctgaa tggaatctat ctttttatgg atttttattt
     3181 gattttcttc tttgtagtaa tgatcaagca gttttgtttt ctttttttct tcaactatgg
     3241 tcagcttttc acgcaaaatc tgataaatct ccgaagattc aaactcatct agaacactag
     3301 gtagataaat caaatcatcc cactcaaaaa taagctcttg ctcagtagtc aaaattgcat
     3361 catattccgt tattttgttt gattgatttt tgaaaagata cactggttct atccggcatt
     3421 ctttgatcca catgttcata ctttgttgaa tctgtttttt tagtgtattc aaaatactaa
     3481 ttggctggtt actaacaatc agtatctccc cttgttttcg aaaaggattc aacgcttcgg
     3541 caagatacaa aaccaaataa gcgctttcag ctaaattggg acgtaattga aaatgtattg
     3601 ggaagaaacg ccgaattaaa taaatttcta aaggataacg ggagatgatc ttttgtacaa
     3661 aatgattcat ggtattatgt ccggcatcca tcctcgtttt catttgtttg atatgggtta
     3721 gcaaaaattc tgtttcatgg aacaataacg aaacaggaag atctaaaaaa cgaataatcg
     3781 tttgctcaaa tgctcttaga ttcttctcta tttctttgtt tttttcatca ttaatcatca
     3841 aataatttaa ctctaatagt cgattttcaa ttgcttgttt ttcttgattg gaaaaagaat
     3901 agggtagttc tgatgataac ttttttgtga gggtctctac catcggaata aataatgtat
     3961 acctgtttgt tttttctaaa agttcttctc ttcgaagaat ggtcaaaacg agataccgag
     4021 aaaatcgtat gaagtcctct cctgaaacaa tataattata tgatataaga acctctttta
     4081 atgtcttttg gacagtcggc atgatattgg aaaaaattgt actgatttgt tgaggcaacc
     4141 ttgcgtgttg gatcaccgtt tctgttccaa caagagataa gaaaattctt tttgaatttt
     4201 cttcagcaat gatttttagt ccttttgtcg acgaaacttc cagttctaat ttaggattgc
     4261 gctcgatcca acgcaaaatc gtttttattt gtaatgatac ggctgttttt gaaagaaaga
     4321 aaatgtctgc tatcttttgc atactgacat accctttttg aaacaataag tacgtcacaa
     4381 tagttgaagc tcggaaatcc atgctggaat agattttatg tttcgtcacc tcttgtatga
     4441 tttgctgttt agtttcagat tcaattgatt ctaaaaaata acctgaacgt ttatttgact
     4501 ggataatgat tttcttctcg tagatagcaa gcatattttt gatttcagtt tggatcgttt
     4561 tcttgttggt acctaaagct tcagctaatt cttcgcttgt tatccaatga ttcttatttc
     4621 ttaatactac aagaatttgg agttgtcgtt tatttaagtc catttgctta cacctcctct
     4681 ttgttataaa catacaggaa aaacctctca aatgacaata gtcacttgca gaggtttaaa
     4741 aatcatttga agaaagatct atattcaagg tttttaccat tagactggat aatttgttta
     4801 taatattcaa aactatcttt tttcaatctc ctaaagcttc cgctcccatc attttgtcta
     4861 tccacataaa taaagccata tcgcttcctc atttcccctg ttcctgccga tacgatatcg
     4921 attggtcccc accataagta accaatgatt tggcaaccat cttttattgc ctcagcaatt
     4981 tcctgaagat gtatttttag atactcacaa cgttttggat cgtggattcg attcaattca
     5041 tctggtgctt catctagtcc aatgccattt tcaactatga ataatggtaa ttcataacgg
     5101 tctgtcaatt cattcatgac ataacgaaac cctagaggat caattggcca actccaaggt
     5161 tcgggagatt tttccttcaa aaagggatta tcgaaaccat ttgctccgcc agtatctacg
     5221 cgcatttctg cttctttttt atagacagcg ctacgataat aactaaatgc aatataatct
     5281 accgtgttag cttgcaagta ctgttcgtct ccatctaaca ttactggtgc acagtcttct
     5341 tccttccata tcctgtaaat ataagctgga taatgccctc ggcacatgac atcggagaaa
     5401 aaccatgaat tgcgtttaaa ttgcagtgaa ccaaatacat tctctggatc acagtcatat
     5461 gcataagtcg ctaatgcaga aaagctacac ataataccca tcttagcatt tggatcgatt
     5521 ttctttaata attgagtagc acgggcgttc gctagaaaca tatgatgaca ggcttgataa
     5581 atctcttttt ttgttagtgt agcaatgggt tcaagtttat ctctagcagg gaatgtaacc
     5641 aatccagcag ctgcataagg catacgaaaa gcattattga tctcattaaa ggtcatccaa
     5701 tatttcactt tcccttggta gcgttcaaag acggtctgcg cataaaattc aaaaaagtcg
     5761 attaattttc gattttgcca ccccccatac tctcctacca atgctaaggg ggtttcataa
     5821 tgactaagag taattactgg ctcgatatca tatcgattta atgtattgat tagtctgtca
     5881 taaaatacga gaccagcctc atttggcttc tgttcgtctc ctcttggaaa tattcgtgcc
     5941 catgagatgc ttgttcgaaa agcttttaaa cctaactcct tcagtaaacg aatatcctct
     6001 tcaaatcgat gataaaaatc aatcgcttca tgagaaagat aatggagtga ttcgtctaaa
     6061 gcaatctccc aaatcttttt ttcttcattc cactgaatac taggatgctg gttcatgatc
     6121 ccgataagcg tatctgtcac attaggtaat ttgccgtctt ccaaccatgc accttcacat
     6181 tgattagcag ctactgctcc gccccataaa aaattttctg gaaatatttc ctgatccata
     6241 actatctctc ctcgctatca acaatttctt acttgaagta tagctcgatt catcaaaaca
     6301 agatattcta actatttcgg cagaagagaa gaaaaaaatt gaatgctact cttcattttt
     6361 aatatttata tataacaaat acataagacg atccccgaaa gaaaacactc gttaaccatc
     6421 taagcaaacg atttatttta cttccaacac aaatgcttca taaggtcgta aatcaacttt
     6481 gccgtctggt aactgtttca gaggataatt tgacacaata acccttaaag cttcacgtgg
     6541 aacagtgaac gtctgctttt cactggacat attacaaata acgagccatt gctggtcttc
     6601 atatatgcga gaataggcaa agacatgctc aggcgtcttt tccactaatt catagtctcc
     6661 ccagacgatg atgtcatgtt ttctacgcaa atcgatcaat ttcttgtaca tataaaaaac
     6721 agaatcttga tctgctaacg cttcttctgc attgatttct ttataatttg gattgactcg
     6781 aagccatgga attccagtag taaacccagc atgttcacgg gcgttccatt gcataggcgt
     6841 cctagcattg tctcttcctt tcgcattgat tgattccaag atttcctctt ttgtaaatcc
     6901 agaagaaatc ctttcgttat acatattgat tgtctcaata tcttttgctt ccgcgatatc
     6961 atcgattggg caattcgtca tgcctaactc ttctccttga tagatataag gggtgccttt
     7021 catcatatgg agcaagatag caaacatctt ggcactttct tttcgataca catcgtcatt
     7081 tccccaacga gaaacaattc gcggaagatc gtgattattc caaaataaac tattccatcc
     7141 ttcatcacct aaagatgttt gccatttgga taatactttt ttcagttcag agatactcaa
     7201 gggtttcaaa tcccatttgt ctttgccttc ttgctgatct aatcctatgt gttcgaattg
     7261 gaagaccatg gataattcat ttcttttagg attggaataa agtttagcga tttcggtagt
     7321 tgctccccat gtttcgccaa cagtcatcag atttttgtca ccaaaagttg cttgattcat
     7381 ttcctgaaga tactcatgca gtttaggtcc attacttgtg atttctttat ccggttcttt
     7441 gccaatcata tcgatcacgt ccatacgaaa gccaccaata cctttatcga tccagaaatt
     7501 catcatttga taaatctctt ggcgaacctg tttatttttc caattcaaat caggttgacg
     7561 cttgctatac aagtgcagaa aatattgccc cgttttttca tccaactgcc aagcagaacc
     7621 actgaatatt gagcgcaatc tattaggctc ttttccattg accggatcac gccaaatata
     7681 atagtcccga taaggatttt ctttattttt acgggcctca atgaaccacg gatgttcatc
     7741 agaagtatga ttcaccacca gatccatgat gatacggata tttcttttct ttgcttcttc
     7801 aatcagttct tccatttctt ccatcgtacc gaattctgcc atgattgctt gataattact
     7861 gatatcatac ccattgtcat cattcggaga ctcgtatact ggagaaagcc aaattgcatc
     7921 aatacctaaa tacgccagat aatccaacct ggaaataatc ccccgcaaat ctccaatacc
     7981 atcttgattt gaatcttgaa aacttcttgg atagatttga taaacaactg aatggtgcca
     8041 ccatttttgt tccatg
//
LOCUS       DACEXQ010000119         8020 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.119, whole genome shotgun sequence.
ACCESSION   DACEXQ010000119 DACEXQ010000000
VERSION     DACEXQ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.119"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..1452
                     /locus_tag="IXH04_001802"
     CDS             <1..1452
                     /locus_tag="IXH04_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ1852402.1"
                     /translation="TIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEV
                     QYTQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTR
                     ELQYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHG
                     CYRSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKI
                     ESGIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILY
                     FTESGEKVRRRKLIANDILIILKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVD
                     LYKFVTKHIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRD
                     GITLTTAHKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKN
                     NLFLFSEVGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            1652..1966
                     /locus_tag="IXH04_001803"
     CDS             1652..1966
                     /locus_tag="IXH04_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018705633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ1852403.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            1987..2364
                     /locus_tag="IXH04_001804"
     CDS             1987..2364
                     /locus_tag="IXH04_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ1852404.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            2374..3147
                     /locus_tag="IXH04_001805"
     CDS             2374..3147
                     /locus_tag="IXH04_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852405.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            3169..4572
                     /locus_tag="IXH04_001806"
     CDS             3169..4572
                     /locus_tag="IXH04_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ1852406.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            4754..5938
                     /locus_tag="IXH04_001807"
     CDS             4754..5938
                     /locus_tag="IXH04_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ1852407.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            5935..6225
                     /locus_tag="IXH04_001808"
     CDS             5935..6225
                     /locus_tag="IXH04_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852408.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            6222..6443
                     /locus_tag="IXH04_001809"
     CDS             6222..6443
                     /locus_tag="IXH04_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852409.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            6485..7264
                     /locus_tag="IXH04_001810"
     CDS             6485..7264
                     /locus_tag="IXH04_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HAQ1852410.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            7331..7825
                     /locus_tag="IXH04_001811"
     CDS             7331..7825
                     /locus_tag="IXH04_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ1852411.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
BASE COUNT         2801 a         1253 c         1542 g         2424 t
ORIGIN      
        1 acaattattg atgattatac tcaaagagct tacgtcagcc aaattaataa tatgctgaat
       61 ttaaatctca aaacgtatga actgaacaaa attgatttgt cactttctca tgattttaaa
      121 attaatgaag ttcagtatac tcaagtttct gaaatgtatc acactatgtt ggaaaaaaat
      181 aaagaaattg attttgatat gattttacta gtagcgtata aaattttaac tgtaaacagt
      241 aacgttgcac gtaatattgc tagtaatata agggcgattt atgtggatga atttcaagat
      301 actagagaat tacaatacaa tgtaatagct aaattacttc aaaacaaccc tcaaattcag
      361 tcaatgtttg ttggagatat tgaccaagca atttatggat cattagatgg tatagctaaa
      421 tcagttgaag aactagaaat gctaactagt cacacttttc aatcaaagac gctacatggc
      481 tgttacaggt ctaatcagag attagtagac ttctactcta attttcagag ttgtccttac
      541 gagattgaaa gcagagggaa taataaaaac gaccgtggtt ttataagtta tgattgcaaa
      601 gttactaagg aggatttacc caacataata aaaaatataa taaaggaaaa gattgaaagc
      661 ggcattcatg aaaaagatat ttgtgtactt gctcctcaac actatcctct gttttcactg
      721 ggtgagtatt taaagaaaga attaccgtat tgcaattttg atgctataaa tatatcacca
      781 attaaggtaa ataatcatag tttatttttc aagttagcaa ttttgtactt tactgaatca
      841 ggtgaaaagg ttcgaagaag aaagctaatt gcaaacgata ttttaattat attaaaaaat
      901 gaatttggaa tagaagttaa agattatttt attgacttag atttattaca gctaataaac
      961 agtgtgaaaa gaatgtatag tggagacgac aacggtgttg atttatacaa atttgttacc
     1021 aaacatatat ttgttaatct taatattgac gagaaaaatt acccgtctat ttttaagcac
     1081 tatctatttt ttattaacga agtagaagat cggataaaaa ataattcatt atctagtagt
     1141 gtatctagtt ttaagaagat gtttaaatct cgagatggta tcacacttac tacagcacat
     1201 aaagttaaag gaaatgagta tgatactgtt atcgctatta atcttctaaa cgggaaaatt
     1261 cctcattgga atgaaatttt taatgtttct gataaaggaa ttagttcagc gagaaaacta
     1321 ctatacgtaa tttgttcaag agcaaaaaat aatttatttc ttttctcaga ggtgggttat
     1381 tttacgagta aaggagactt gctttatccc actcaattat tagataaaat agaatttgat
     1441 tataatattt aaaataccta ggtagtcgtt gttttaaata tagtaggaat cggacgctca
     1501 gtacggagcg tctatttttt tacccaattt tgaaaggaag tgaacttatg aaaataaaaa
     1561 atcaagaatc aaaaggtcgc tctcccccct ttaaaaccat cacacaaata agattcattc
     1621 aaacataaaa acagaaagga taggtaaaaa tatggaactt aaatatgtca ttcccaatat
     1681 ggaaaaaaca tttggacaat tagaatatgc tggtgaaggg aacattgaac aacgtcgaat
     1741 aaatggcaga ccaaccattc tctctcgtag ttacaattta tactcaacga ttcaacgagc
     1801 agatgatatt gttgtcattc ttccctccga agctggcgag aaacattttg aatccgacga
     1861 aaaagtcaaa ctcatcaacc cacatattac cgcagaaggt tataaaattg gcaatcgagg
     1921 atttaccaat tacattatgc acgcagacga tatggtgaaa gcataagaaa ggaaaggtga
     1981 aaaaatatga gattagcaga aggtattgtg attgaaaaag accagacatt tggcacattg
     2041 aaattttccg ccttgcgtcg tgaggttcgt atccagaatg aagatggtac ggtttccgaa
     2101 gaaattaaag aacgtaccta tgacttaaaa tcaaaaggac aaggacgcat gatacaagtc
     2161 agtatccccg cttctgttcc tttgaaagaa tttgactaca acgcagaagt ggaattaatc
     2221 aatcctgtgg cagataccgt agcgacggct acttttcgag gggcagacgt tgattggtac
     2281 gtgaaagcag acgatcttgt attaaaagga cagacaaaac ctcatcagaa atataaaaaa
     2341 gaagcgtccg aaagtgaaaa ataggtgttg cttatgacaa acacaccatt gattttgaaa
     2401 gaaattccga aggatgaagc catttctttt attcggcaat accattattc aaagatactc
     2461 cctagattgt gtaaatactt tctagggatt ttttcagaag aaaaattatt aggcgttgtg
     2521 gaattaggct ggggtacgca acccctacaa accatacgca aactctttcc cgacagttcc
     2581 cttcaaacca cagattattt agaaattgga aagatgtgtt ttcttccaga aatgaatcaa
     2641 accaattatt ttggcagtca agctctttca gctctaatta agtggttgaa agaacatacg
     2701 gactgtcatt ttttatatac actggcagat ggaattgagg gaaaatgtgg ctatgtctat
     2761 caagcgtcca acttctttta ctgcgggtat ttcaagacaa gtgtctatcg tgataagcag
     2821 tcatgggaaa agatacaccc tcgaagtgct cgccttttat tagaggaaaa tgctcgcttt
     2881 gaacaggtag agaaaaagca ttggctttcc caggcatttt gcgaatacaa agggattgag
     2941 aaaatcaatg gacgcatgtt tcgctatctc tatccgttga caaaagaagc gaaaaaactg
     3001 ttaggacaca ctctctatcg acgtcattat tatccgaaag aaaaaaattt acgctttgaa
     3061 aagcgaatcg cttatcagaa atatgaagcc atttctcaac caacttttga taaacaggct
     3121 cgtatctaca atacacaatt attttgatag gtggaaagga ggatttttat gccattgctt
     3181 catgacagag gagagcgaat caaagccaac gataagcact tggtctatca ttttgtacta
     3241 atttcattgc tggtagtttt tctagtcgtc ctactccctt ttcattggag agcgttacaa
     3301 tcggtgaact ggcaagcagt tcagttagat acatttttct atcaacttca tacgccatac
     3361 ttttggagca gtattctcac tgctcttttt gtatgcggac ttatcagctg gctttattat
     3421 cactatcgta tagattctat aaaacagtta gagcaccgtc aaaagttagc tcgtatgatt
     3481 ttagaaaatc attggtatga agccgaagaa actcaatcag agggattctt taaagattta
     3541 cccagcaaga aaaccaaaca acgaatccgc cattttccta aaatatatta tcgaatgaaa
     3601 gatggactaa tccatattca tgtagaaatc accttaggga aatatcaaga tcagcttttg
     3661 cacctagaaa acaaactaga aacaggattg tattgtgaat tagtggaaaa aacgttgaaa
     3721 gattcttatg tagagtatgt cctactttat gatactattg gcaatcgaat tggcattgaa
     3781 gacgtggtag ctcaagatgg acgtgtcaaa ttgatggaat cggtctattg ggagttcgat
     3841 tctctccctc acatgttaat tgctgggggc actggtagtg gaaaatccta ttttattctc
     3901 acattgattg aatctctcct gcatacaaat gcaaagcttt atattcttga ccctaaaaat
     3961 gctgatttgg cagacttagg ggcggtcatg aataatgtct attaccgtaa agaagatatg
     4021 ttggcatgta tcaatcaatt ttacgaagat atgattgatc gcagtgaaac catgaaacaa
     4081 caccctaact ataaaacagg agaaaattat gcttatcttg gtttgtctgc caattttctt
     4141 atttttgatg aatatgtggc aatgatggat atgctaggtc gtgaaagtac caccgttatt
     4201 cataaactca aacaaattgt aatgttagga cgacaagcag gcttcttttt aattcttgcc
     4261 tgtcaacgtc cagacgcaaa atattttagt gatgggattc gggacaactt taacttccgt
     4321 gtggctttgg gtcgtatgtc tgaaatgggc tttggtatga tgttcggaag cgatacacaa
     4381 aaacaatttt tcttaaaacc catcaaggga agaggttacg tggatacagg aaaaagtgtg
     4441 attagtgagt tttatacacc ccttgtacca aaaaggtacg atttcttagg agaaatagga
     4501 aaagtcattc aaaaaaagca atctgagcct gtaccgcacg aagtgaaagg tacaggttca
     4561 gacttgcttt gattggtgtg gcgttagcca cgccaatcct ttagccccct ctatctaaca
     4621 ggggggcaca aatacaactg aaaacaggca attctcatca accaatgttg atggtacaaa
     4681 ggattgcgga aaatatgaaa ggtgtaaact caagggtcat agtttacatc ttttttagat
     4741 tggaggtttg cgaatggaaa acgaaaaaat ttggattaag gaattgaaag aaaagcgact
     4801 ggcttatggc gtttctcaaa ataaactggc ggttgcttct catattacca gaccttacct
     4861 atcggatatt gaaacaggca aggcagtccc tactcaacag gtgaaagaag atttactgaa
     4921 cgctttggaa cgcttcaatc ccgataaccc attggagatg ttattcgact atgtacggat
     4981 tcgattccct accaatgatg tggctcaaat tatcggtgag gttttacgtt tgaatatgga
     5041 ttatatgcta cacgaagact ttggctatta ttcttatccc gaacactatc gtttcggtga
     5101 tattgttgtc ctagtctctc atgatgtaag taaaggtgtt ttattagaac tgaaaggaaa
     5161 aggctgtcgg caatttgaaa actttctatt ggctcaacat cggagctggt atgatttctt
     5221 tcaagattgc atggaacata aaggtatttt caaacgcttg gatttagcta tcaacgataa
     5281 aacagggatt ttgaatattc cagatttggc aaagaaatgt aaacaggaag aatgcatttc
     5341 cgtctttcga agctttaaaa attatcgcag tggtgaattg gttcaccgtg atgaaaaacc
     5401 atacatggga aacacacttt atattggctc attaaaaagt gaagtctatt tctgtatata
     5461 tgagaaagat tatgagcaat acgtgaaaaa tgatgttccc ttagaagacg cagaagtaaa
     5521 gaatcggttt gaaattcggt taaaaaatga ccgagccacc caagccatga aagatttatt
     5581 ggcacatcaa caggcggaaa aaacagcctt tgaaattatc aatcgctata tccgctttgc
     5641 agataaagac gacactaaac gtcggagtga ttggaaaact aatgaacgtt gggaatggtt
     5701 tatcggtaaa aatcgtggag cattacgcct aaccacacag ccagagcctt attcttttga
     5761 acgaacattg aactggttac atcatcaagt cgcccctaca ttaaaaatcg caagcatttt
     5821 agatgttctc aacggtacaa ccattatctc caccatgatt caggaagcca aactgacaga
     5881 aaaacatgag aaattaattg aacaacaaca tctagccatg gaggatttga ttacatgagc
     5941 cttttaacaa aagaattaaa gaagttaggg tttcagtgtg gcattgagtt tcaagcgtat
     6001 atacaaaata cagggaaata tacgtctctc attattgagg gaaaacggca agctggtgat
     6061 accatctata cctatgattt ttacaaggtg agattttata ataattatac caatcgagta
     6121 accgtctatg gagagcattt aacccctttt caattattaa ggcgtgtaaa aagctatatc
     6181 tattatcgag aaaaatatct taaggaaagg aggacaatca catgaatttt gggcagaatt
     6241 tgtacaactg gttcttggat aatgcacagt cattggtatt gttagcgatt gttgtcattg
     6301 gactgttttt aggttttaaa cgagaatttt caaaactcat tggcttcttg attattgctc
     6361 taatcgccgt cggacttgtg ttcaatgcag ctggcgtgaa agatgtgtta ttaaacttat
     6421 ttaaccgaat cattggtgct taacagatat ttttcatgat aaaataggaa gtatatattt
     6481 tgttatgaaa ggatttatga atatgtttac aaatgatcaa tttaaatctc ttttaattaa
     6541 aacattagaa agaaagagct cttcaaatta ttatgaagga ggtaacgaga ttgtttctaa
     6601 aatgaaaata tcagaggtaa ctttagatga gactgacgat tttacttatt ataaaggata
     6661 taaaaagggc tggaatacgc tttgcacata tcttaaaata agagttcctt ttgatgattt
     6721 agactttttt gaaagtcata aagatttgat tacccagaca gcaagctcca tatatgataa
     6781 acaaggtgat aatgtattag tggatactat tttagtacca cttccagaga attacgaagt
     6841 aattaacttt agtcagttga aaattagtga tgtggtttca caagctattg aagacgctga
     6901 gtcttttatg agtaatggtg aatatcaacg tgcctttgac agagttcaca ctgcttttca
     6961 tggatattta attgaaatac taaaaaaata tgaaattaca gttcctagag acgaaaatct
     7021 atctaaatta tatagtagga tacaacaatt aattgaaaaa gaaattcaac ctactgaact
     7081 tgctgatata gtgaagacaa cgataaggtc atcaaatgga atgattagct ctcttaatga
     7141 agcaagaaat aggcattcac ttgcacaccc taatacaaat attattggaa agagagaagc
     7201 aaaactaata ataggtattt caagtacagt tacagattat attagcggat accttgataa
     7261 gtagcgtcat taactgattg ctactttttt tatgactatt tttataaaaa ggatgtgata
     7321 agaattggaa atgaaagtct atgtggcaaa tttaggacgg tataatgaag gtgaattagt
     7381 cggagcatgg tttactccac caattgatga ggaagaaatg gcagaaagaa tcggtttaaa
     7441 tgaggactac gaagaatatg caatacatga ttttgagctt ccttttgatg tggacgaata
     7501 cacgccgatt agtgaaatca accgtctatg tgaagccatt caagaaatcg aaggtacacc
     7561 tatttataat gaactcaaag aaattcaagg catgtggttt agtagcttgg aagaattact
     7621 ggaaaacaaa gaggatattc attgttatag tgattgtgat tcgatggaag atgtcgcccg
     7681 ttattacgtg gaagaaacag gacagcttgg tgaagtccct tccaatttac aaaattatat
     7741 tgactaccaa tctttgggac gtgatatgga aatagagggt aattacctag ttacctctca
     7801 tggggtattt gaatattgtc agtaagttta acaatccgtg ttataattaa attaataaat
     7861 agcacggaga ggtataaatt atggtaaatg tatttgaaaa tcttaaacta ttaaaactag
     7921 acatgaaaga taaaggctgg gtgatagatt ccttttactt tcgttacaaa cagcaaaatt
     7981 atattgtact tgttaagcta tttgaaaaag aggaaaaagt
//
LOCUS       DACEXQ010000120         7951 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.120, whole genome shotgun sequence.
ACCESSION   DACEXQ010000120 DACEXQ010000000
VERSION     DACEXQ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7951
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.120"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(743..2089)
                     /gene="gor"
                     /locus_tag="IXH04_001812"
     CDS             complement(743..2089)
                     /gene="gor"
                     /locus_tag="IXH04_001812"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAQ1852412.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(2223..3464)
                     /locus_tag="IXH04_001813"
     CDS             complement(2223..3464)
                     /locus_tag="IXH04_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ1852413.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(3482..4405)
                     /locus_tag="IXH04_001814"
     CDS             complement(3482..4405)
                     /locus_tag="IXH04_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ1852414.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(4626..7118)
                     /locus_tag="IXH04_001815"
     CDS             complement(4626..7118)
                     /locus_tag="IXH04_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAQ1852415.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(7125..7592)
                     /locus_tag="IXH04_001816"
     CDS             complement(7125..7592)
                     /locus_tag="IXH04_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAQ1852416.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(7728..7801)
                     /locus_tag="IXH04_001817"
     tRNA            complement(7728..7801)
                     /locus_tag="IXH04_001817"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7765..7767),aa:Arg,seq:acg)
     gene            complement(7859..7932)
                     /locus_tag="IXH04_001818"
     tRNA            complement(7859..7932)
                     /locus_tag="IXH04_001818"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7896..7898),aa:Pro,seq:tgg)
BASE COUNT         2219 a         1721 c         1284 g         2727 t
ORIGIN      
        1 ccacattttt gcataaattt ggcttaatac cgagaaactg gcttttgaac accgtttatc
       61 agaggttgtg aaaaagtcgc ttagtttcga gtattaagaa aaattttgtg aaaatggctt
      121 tccacatttt tgcataaatt tggcttaata ccgagaaact ggcttttgaa caccgtttat
      181 cagaggttgt aaaatcagtg ttttgtcctg agtattagag aaacaaatgg aaaaatagtt
      241 cctcatattt tgccgttttt tgagctaata ccgaaggaca gctgattgaa cactatttgt
      301 tcggaaaaag tgactaacta tgacacaatt tttttcacag tccctttttt cacttgattt
      361 gattcgtacc agcgaacttg acgagaactt tcttggtttg gtaactatat ggtattcttt
      421 taacatcctc gtttgcttct tctaatttat ttatacaaag tcacactttt ttaaacatct
      481 gttcttcgat acatttctat ttcttttaaa catcgatacc aatattttga acttaagagt
      541 gcataccaaa tggattcatt tactcttcgt cagttacgaa gataatcaaa atctccaact
      601 tccctactta tttcgttttg agaattatcg aagcaatctt acataaataa ctaaaaaaca
      661 aaaaaccaga aaatcagtat actgattttc tggttttaca tggtttgttc aataaaaagc
      721 tgttggaact gttaatatta aattatcgca tcgttacgaa ttcttccgct cctgttggat
      781 ggatggctac tgtattgtca aagtcttctt tagttgcacc catcttgata gccacggcaa
      841 aaccttgcag catctcatcg acaccaacac caatagcatg taagccgacg attctttctt
      901 cttttccgac acagatcaat ttcatttcgc atttttggcg ataatcattt aatgcaaagt
      961 acattggggt aaagcgtgaa cgataaactt tcaattcatt ttcgccatat ttttccaatg
     1021 cttcttcttc tgtcaaacca attgttgcaa ttggtggatg cgtaaagacg actgtcggaa
     1081 caagtttata gtcaagataa gaatctttct ttccattaaa taaacgttct gataaacgtc
     1141 taccagctgc gattgcaaca ggagtcagat ctaatttacc gatcacatca ccaacagcaa
     1201 aaatatgttc atccgttgta ttttggtatt tatctacttt gataaaccct tttttgtcca
     1261 attccacatt tgtattttcc agacctaatt tttcggtatt cggtacacgt ccaccggcaa
     1321 aaagtacaca atcaccagtt aaggtttcac cattttcaaa tagcaccgta tattcgtcat
     1381 tgtttttagt aatttcttta gcaatatgat gtgcgtaagt ttttattcca gcttcttggt
     1441 acatttggat caaattgtcc gaaagcattt tatcaaacgt tctaagtggt cgttcttttc
     1501 gatatgccca catgacttca ctacctaaac cacgaaatac acctgaaagt tcagccgcaa
     1561 tgtatccagc acctaaaacg attgctctct taggtaactc tgttaattca aaaaatccat
     1621 ttgaatcaat tgcgtattct ccaccaggaa tggtcatttt acttgcacgg cctccagtag
     1681 cgatcaagat tttaggagca cgataggttt caccgtttac ttcgatcgtt tgactatcga
     1741 taaaagtagc gtaacctttt atagcctcga catgattgct atctaatcct ctttgataag
     1801 caccgtgtag aaaatcaata tatttttctc gtctttctac tagaccggcg aaatcaacat
     1861 tttttaaatc tgcttggata ccgtaacttg aggcatctcg ttcaattgtt tccaaaatcg
     1921 tacttgcttg ccacataact tttttaggta cacaaccgac gttgacacat gttccgccca
     1981 attcattcgc ttcaatcagc aaaacttttg cgccatgcat ccctgctctg tttgctgaag
     2041 caataccgcc agagccgcca ccaattacga tgtaatcata tatttgcatt ttgttcctcc
     2101 ttattgaaga gatcaatcaa aacctttatt taacgatcct ttttctacag aaaacaagtg
     2161 taacactttt cttgaatttt gtatgcgaag atgcttaaaa cagggatata tcagcttttt
     2221 ccttatttac gttttcggat catatctcca ggtttcaccg gctgtgcgac tggcatagtt
     2281 aaaatcatca ttggatttgg tgctcggtca atggattcgc cttcttcatt atgcataact
     2341 tcaacggttt gactgaaatg actgaatcct ggaccataaa attcgatttc atctcctaca
     2401 gaaaacaagt ttctctgacg tatcgtagca atctgtgttg cctcatcata agataatact
     2461 tcaccgacaa atttatatac tgggatttta cgacgttcac cgaataattg ttcgttttca
     2521 gttggtgtat tgtagtaaaa acctgtagct aattctcgtt gtgctacttt ccataactcg
     2581 tcgatccatt cttgcttgca gacatagttt tcaggatctt ccatataact gtctacagcc
     2641 gctttataga catttgacac cgtagataca taatggatcg atttcattct tccttcgatc
     2701 ttgaagctgt ctacgccgtt ttcaatcaat tctggaatat gttcgatcat tgacatatcc
     2761 actgcactca tcgagaattc ttcttccact tcgcctgtat cagttaaact gtttcgttct
     2821 tgaccaaaag gcatatcgaa caatccatac ttccagcgac aagattgaga acatccgcca
     2881 cggtttgcat cccgcatcga catatggttg gacaatgtgc atcgaccaga ataagagata
     2941 cacatggcac catggataaa tgcttcgatt tcggtatccg tatttttacg aatctctgcc
     3001 acttcctcca ttgaaacttc acgagctaaa acgacacgtt ccaacccttc ttctttccag
     3061 aattccaatg tctcaaaatt agttgctgaa gcttgagtgg ataaatggat cggcagacct
     3121 ggggcttctg tcgcacaaat ttcgatcaaa gctggatcag aaacaatcac tgcagaaata
     3181 ccgacatcac gcaaactgcg gaaaaattcc cctgctccat cttcatttcc ttcatgggtg
     3241 accatattag cagcaacgta taccttcgca tcatgttctt ttgcaaaagc aactccttca
     3301 gccatttctt cataagtaaa attacctgca cggcttctta aaccatatgc attcccgcca
     3361 atatatactg catctgctcc ataatggatc gcggttttta acttttccag tgttcctgct
     3421 ggcgctagga tttctggtcg tttcaatttt ctttttactg tcatttcttt catcatactc
     3481 cttatttgat agcagaagga tcaatataaa agaatcctgt atctaattca cggttttcag
     3541 gatgcaattt actgatttcg ctagtcattt tttccgcttt ttcttgatcc cagctacctt
     3601 ctaaaatttg tttttttgct tcggcgaata gtcgagtgat gtttacgaat ttttcgcctg
     3661 gagtaaataa tccgtccaat ttccatgtac agtaatgatg ctgataaagt tcattcaatt
     3721 cattcgagag attcagatca ttgctggcaa aaatatgtgt gccatgacta tcttcataaa
     3781 tagaataatg tgtctcttct tttttaggtt ccgagataaa aagacctctt tcacgatctt
     3841 tttgttcgtc ttgtttcgta tagttatagt aattctgtag cagtggacgt ttggattgat
     3901 ggatgcatgt agcaccatac acaagggttt ctacaggaat atcgagtttt tcttccatcg
     3961 ccttcatctc ttcaaaagga acttcacgag caagtactgc tccaactgcg ccttttttcg
     4021 cccagaaatt gatttgacgt gcgctcgtta ctaatgtttc agcatcataa atatacggta
     4081 tacgcaattc aggatttttt ttcatcgtat aaataatacc tgtatcacca actgtgatca
     4141 catctacgtt tatttctttt aaaaaagcca aatattcagg tacatttttc attttttcag
     4201 gatgaatgat cccatttaca gcaatattcg cttttttacc atacttgtgt gctaattcta
     4261 ccaattgtcg ctgttcttct cgtgtaaatg aagcaggcag acgcaatcca aattgctctt
     4321 caccgaaata tagtgtatct gcacctgcag caagcaactg ttctgcttgt tcaatagttt
     4381 ctactgtggt tatgagttcg atcataattt tctcctccta caacttcata atagtagcat
     4441 agctctaaaa actttccaat acttcttcta tttaaagtcg gatttttgaa aattatttca
     4501 caaaaaatca aaataaagtt cgatatattc tgtccaaata taaaaagaga ttgtgacaaa
     4561 aatcttgtca caacctctta atcaagtctt actattataa aaattcagct acacaggcca
     4621 agtaactata gggaacctac tgtttccttt ttaggtccgc gaacattcaa tgtgatctta
     4681 ccttttgaag cacctactgt tacgtggtca cctaaacgaa tttctccgcc aagcaaagcc
     4741 tcgctcaatc gatcttcaat ttctttttga agtgcgcgac ggattggacg tgcaccatat
     4801 tcaggatcaa atccagcttt cccgatcaca tcgattgcag atggtgtgat tttcacttga
     4861 atatcttgtt ctttcaatcg tttgataatc gctttgctca tgatttggac gattgtatgg
     4921 atttcatctt tactcaatga atggaagacg acagtctcat cgattcgatt caagaattca
     4981 ggtcggaaag ctttctttaa ctcttctaag atacgttttt gcatggcttt gtgatcttta
     5041 gtaacatctt ggacattgaa tccgacattt ttttcttctc gaatttgtgt cgctccgata
     5101 tttgaagtca taatcatgat tgtatttcta aagtccactt ttctaccttt agaatctgtt
     5161 aaatgaccat catccaatac ttgcagaagt aaattgaaaa cgtctggatg tgctttctcc
     5221 acttcatcta agagaatcac tgaatatggt ttttgacgaa ttttttctgt caactgtcca
     5281 ccttcatcat accctacgta tcctggagga gacccgatta atcggcttgt actgtatttt
     5341 tccataaatt ctgacatatc aacacggatc agggcatctt cacttccaaa catgacttct
     5401 gaaagtgctt tggccaattc tgtttttccg acaccagttg gtccaaggaa catgaatgaa
     5461 ccaattggac gatcaggatc tttcaatccg cttcgtgcac ggcggatcgc acgtgaaact
     5521 gctttgacag cctcatcttg accaacgaca cgttcatgca gaagtccttc cagttctaac
     5581 aagcgttcgc tttctttttt ctccaactgc tgtagaggga caccagtcca ctccgataca
     5641 actgtagcaa catcttcttc tgttacacga tcagaataac cttgctcctg ttttgcttca
     5701 attgcagaaa ctttttgcag ctttttacgt agtgcttttt cttgtctgcg taattgagca
     5761 gcattttcaa agtcttgttt ttggatcgct gcttcctttt cttgtactag ttcatcgatt
     5821 tctagtttga tcacagttga ttttgttaag tgatcggttt gatccaaacg gactttcgct
     5881 gctgattcat cgatcaaatc aattgcttta tcgggtagtt gacgagaagt gatataacga
     5941 acagaaagat tgactgctgc tcgtacagct tcatctgtga tctccactcc atggtgctct
     6001 tcatatcttg tacgtaatcc ttgaagaatc acttctgctt cttcaggagt tggctcatct
     6061 acttgaatgc gagcaaaacg tcgctctaat gcagaatctt tttcgatata tttttgatat
     6121 tcatctaggg tcgttgctcc gatcgtttgt aattctcctc gagctaacgc cggtttcaag
     6181 atattcgaag catcaattgc tccttctgct ccacctgcac caattaaggt atgcaattca
     6241 tcaatgaata agattacttg accatcatta tagatttcgt cgatgacctt tttcattcga
     6301 tcttcaaatt caccgcgata tttcgtacca gcaaccaaag atcccatatc aagcatcatc
     6361 aggcgctttt cttgcatgtc ttctggcact tcacctaaaa tgattttttg agctaaccct
     6421 tcagcaatgg ctgttttgcc tacaccaggt tcacccacca gtacagggtt attctttgtc
     6481 cgtcggctaa gaatctggat aagccgtttc acttctctcg aacgaccaac aactgggtcc
     6541 agcttgtttt cttttgctaa tttcgtcaaa tcacgtgcta atgaatctag cgtaggtgtt
     6601 ccttgcggtg tttgccgttt atcttgtaca tttacatttc tacgacgatt cattcctttt
     6661 ccagcagaat caccagctag ccccattttt tgtttcagaa cttgacgggc ttttgaaagg
     6721 ctgataccta agttgatcat gattcgagta gccatgatac tttcatcttt taataagctc
     6781 atcaaaatat gttctgttcc aactttttga gaaccagttt tcttcacttc gctgctagct
     6841 aaagcaaaaa cttgtttcat tcttggtgaa tatggaagaa agactccttt agggtaagaa
     6901 cggataccac cataaccaat cagatgttca atttcttcat aaatttccgt ttcattcaaa
     6961 tccatttcac gtaatacttt tccagcaatt ccattttgct cgaccactaa ggctagtaaa
     7021 acatgttctg aactaactga ttgatgtttc aaccgttttg cttcttcttg tgcaatttct
     7081 aatactgctt gggctctttc agtaaaaagt tcttccatct aatctcactt cccatcttca
     7141 taacttaaac gttccagaaa tgctcggaga atccgagcac gtgtatgatc ttcatattca
     7201 ttgaaattca atgtgttttt tgcaattgca ctcaacatca agtttccttc tttttttgta
     7261 attatttggt cttcataaag tttttgaata atagcaaaag catttttctc actgattgta
     7321 tcatcaaata actgatttat ctgctcaagt aattgttttt tatctgaaat ccttactttt
     7381 gcaattcgta tataaccacc accaccacgt ttgctttcta ctagataacc acgttggatg
     7441 gtaaaacggg tattaatcac atagttgatt tgtgaaggaa cacagttaaa gaggtctgcc
     7501 atttcaatcc ttcgaatctc gatcatttca ctattttcca gaattttctt tatatatgat
     7561 tcaattaaat ccgaggtatt ctgatgtgcc atcttattca ttccctttga ctaatattga
     7621 ccttaattat aacgaatttg ataatagaaa gaaagaagaa cgctttttgt taaataaatc
     7681 ctttagaatc aacaaaaaaa aatacggaat atactattcc gtatcgacgc gcccaagagg
     7741 agtcgaaccc ctaacctttt gatccgtagt caaacactct atccagttga gctatgggcg
     7801 catattcaat ttaaaaaaat tataaatcat ttgaaaacaa aaagcaagat aaaaaaaatc
     7861 gggaagacag gattcgaacc tgcgacccct tggtcccaaa ccaagtgctc taccaagctg
     7921 agctacttcc cgtttaaaaa ataaaaaaga c
//
LOCUS       DACEXQ010000121         7901 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.121, whole genome shotgun sequence.
ACCESSION   DACEXQ010000121 DACEXQ010000000
VERSION     DACEXQ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7901
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.121"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..1023
                     /locus_tag="IXH04_001819"
     CDS             <1..1023
                     /locus_tag="IXH04_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAQ1852417.1"
                     /translation="ELKPKHERQNFLVGDDRLDHAVAFLWKDPQTGETVGASYQGTIV
                     DFNRFGKRGTYKHIDKNPTPNHGFNLKIGDPKHLKFFESSIDLLSYAALNREKLQDAW
                     LVSMDGLKHHVISHYVEESISELRRKQTFPQSIEICVDNDRAGHIFYEKEQMKGIVDP
                     FTNKKIRCERGIPNDWQVPKEYKATYEAVAKEMSVEPEAIMAIHKTETNLQLTNQLVS
                     AHDVQSTFGKMLAKGEPVETIDLKEACTTVAKELKVCERADGTYNFDRFYSRKANIKD
                     VNAGILLSYKAEQYYKGYKKHEHEFVPEVKKDWNDQLKHEIQQQEIRKQKRAMLFQQG
                     RQQERE"
     gene            1034..3043
                     /gene="topB"
                     /locus_tag="IXH04_001820"
     CDS             1034..3043
                     /gene="topB"
                     /locus_tag="IXH04_001820"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ1852418.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIEKVPLDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKRSEGFI"
     gene            3389..3985
                     /locus_tag="IXH04_001821"
     CDS             3389..3985
                     /locus_tag="IXH04_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1852419.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDPEKTLCDIWNPWYNVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            3998..4897
                     /locus_tag="IXH04_001822"
     CDS             3998..4897
                     /locus_tag="IXH04_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAQ1852420.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNDIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKERKLAYQRHQQMNREMER"
     gene            4900..5031
                     /gene="ssb"
                     /locus_tag="IXH04_001823"
     CDS             4900..5031
                     /gene="ssb"
                     /locus_tag="IXH04_001823"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ1852421.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGN"
     gene            5053..5385
                     /gene="ssb"
                     /locus_tag="IXH04_001824"
     CDS             5053..5385
                     /gene="ssb"
                     /locus_tag="IXH04_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ1852422.1"
                     /translation="MIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEV
                     VADNFQLLESKAATESRAHADQSSTSPSTATFEQRNTATPNNNGLNASQNPFGGQSID
                     ISDDDLPF"
     gene            5430..5663
                     /locus_tag="IXH04_001825"
     CDS             5430..5663
                     /locus_tag="IXH04_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852423.1"
                     /translation="MDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVFVYLVCSYL
                     LKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(5822..5944)
                     /locus_tag="IXH04_001826"
     CDS             complement(5822..5944)
                     /locus_tag="IXH04_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAQ1852424.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            6134..6358
                     /locus_tag="IXH04_001827"
     CDS             6134..6358
                     /locus_tag="IXH04_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852425.1"
                     /translation="MIKTQPLLVRFTLRTSHEPINCVVANIEIADKLLILEEGKYTIS
                     VTGHFNKRKQLVIESMQIRNPDHFTRSMSI"
     gene            6801..7007
                     /locus_tag="IXH04_001828"
     CDS             6801..7007
                     /locus_tag="IXH04_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAQ1852426.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            7007..7258
                     /locus_tag="IXH04_001829"
     CDS             7007..7258
                     /locus_tag="IXH04_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852427.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEVLLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENLETYENEYWLTKLEN"
     gene            7439..7711
                     /locus_tag="IXH04_001830"
     CDS             7439..7711
                     /locus_tag="IXH04_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852428.1"
                     /translation="MNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQ
                     ALELTYAKFIETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
BASE COUNT         2860 a         1381 c         1653 g         2007 t
ORIGIN      
        1 gaactaaagc cgaaacatga acggcaaaat tttttagttg gagacgaccg tttagatcat
       61 gcggttgctt ttttatggaa agatccacag acaggagaga cagtaggggc aagttatcaa
      121 ggaacgatcg tcgattttaa tcgtttcggt aaacggggaa cgtataaaca cattgacaag
      181 aatcctacgc caaatcacgg atttaattta aaaataggcg atccgaagca tttaaaattc
      241 tttgaaagta gtattgatct gttaagttac gctgcattga atcgggagaa actacaagat
      301 gcctggttag tttcgatgga cgggttaaaa catcatgtaa tcagtcatta cgttgaagag
      361 tcgatttctg aattaagacg aaaacaaaca tttcctcaat cgattgaaat atgtgtggac
      421 aatgatcgtg caggacatat tttttatgaa aaagaacaga tgaagggaat cgttgatcct
      481 tttaccaata agaaaatccg ttgtgagcga ggaattccaa atgattggca agtacctaaa
      541 gaatacaaag ctacttatga agcagtcgca aaagaaatga gtgttgaacc tgaagcaatt
      601 atggccatac acaaaactga aactaattta cagctgacaa atcaattggt gtctgctcat
      661 gatgttcaat ctacgtttgg aaagatgttg gcgaagggcg aaccagtaga aacaattgat
      721 ttgaaagaag catgtaccac ggtcgcgaag gaactgaaag tatgtgaacg cgcagatgga
      781 acatacaact ttgatcgttt ttatagtaga aaagccaaca taaaagacgt taatgcagga
      841 atccttcttt cctataaagc agaacaatac tacaaaggct ataaaaagca tgaacatgaa
      901 tttgtacctg aagtgaaaaa ggattggaat gatcaattga agcatgagat ccagcaacaa
      961 gaaatcagaa agcaaaaacg tgcgatgttg tttcaacaag gcagacaaca agaaagggag
     1021 tgaagtcgtc actatgaaaa ccgtcatttt agcagaaaaa ccgtcacaag cgaaggctta
     1081 tgccgatagc ttttctaaag ctacccgaaa agatgggtat tttgagatcc aagatcgatt
     1141 gtttacagga gaaactgtga tcacgtatgg ttttggtcat ttagtcgagt tagattcgcc
     1201 cgatatgtac gatgaaaaat ggaaacaatg ggcattagaa catttgccta ttttccccaa
     1261 tcaataccac tatcatgttc ctaaagataa gaaaaagcag tttagtgtcg tcaaacgaca
     1321 gctgcagtca gctgatacga ttgtgatcgc gactgatagc gatcgtgaag gggagctgat
     1381 tgcctggtca atcattcaac aagcaggcgc caatcaagga aaaaccttta aaagactttg
     1441 gatcaactcc ttagaaaaag aagccattta tcagggattc caacagttac gagacgcaga
     1501 agagacctat ccaaaatttg aagaagtaca agcacgtcag atcgccgatt ggttaattgg
     1561 gatgaacggt agcccactgt atagtttact cttgcagcaa aaaggcatac caggaagttt
     1621 ttctttaggt agagtccaaa ccccaacact ctatatgatt tatcaattgc aggaaaagat
     1681 tagaaacttc caaaaagaac catattttga aggaaaagcc caagtaatcg ctcaaaatgg
     1741 cgcatttgat gcgaagcttg atccaaatga aacccaagcg acacaagaag catttgaagc
     1801 ctatttaaaa gaaaaaggtg ttcaattagg aaaacagcca ggtacgatcc ttcaagtcga
     1861 aacagagaaa aaaagtgcgg ctagcccacg tcttttttct ttatcgagcc ttcagtctaa
     1921 aatgaatcaa ctcatgaaag ctagtgccaa agatactttg gaagccatgc aagggttgta
     1981 tgaaggaaaa tatctaagct atccgcgaac ggatacccct tatattacgg aaggggaata
     2041 tgcgtatctg ttggatcact tagacgaata caagcatttt ttaaaagccg aagcgattcc
     2101 gactcctatt catacaccga atagtcggta tgtgaataat aagaaagtcc aggaacatta
     2161 cgcgatcatt cctacaaaaa cggttatgac agcagcggct tttgaacaac taagtccact
     2221 gcagcaggct atttatgaac aagtgttaaa aacaacggtg gccatgtttg cggaaaagta
     2281 tacctatgaa gaaacaacga tcttgacgca agttcagcag cttcagttaa aggcaatcga
     2341 aaaggtgccg ctagatctag gttggaagaa gttatttgga aaagagagcg acggaaaaga
     2401 gaaggaagaa gagccgctac ttcctaaagt gacaaaagga gaaatggtga cggtggatct
     2461 acaagttctt gaaaaagaaa ccaagccacc acaaccttat accgaaggta cgttgatcac
     2521 tgcaatgaaa acagctggga aaaccgtgga tagtgaagaa gcgcaatcca tattgaaaga
     2581 agtggaagga atcggtacag aagcgacacg ggcgaatatt attgaaacct tgaaacaaaa
     2641 ggaatatatc aaggtagaga aaaataagct cgtggtgacg aataaaggga ttttgctttg
     2701 ccaagccgtg gaaaaagagc cgctgcttac gagcgccgaa atgaccgcca aatgggaaag
     2761 ctacttgtta aagatcggtg aacgaaaagg aacacaaacc acttttctag ccaatatcca
     2821 aaaatttgtc tctcacttat tggaagtcgt accagggcaa atccaatcca ctgattttgg
     2881 atcgacgtta caagaagtga aagcagccag tgaaaagcaa gaagccgcgc gtcatttagg
     2941 aatttgtcca aaatgccagg aacaagaagt cttactctat caaaaggtgg cagcttgtac
     3001 gtctgaagcc tgtgatttta aaaggtcaga agggttcatt tgaagcaacg attgttttaa
     3061 aagaagactt cactacaggc tttgaatttt ctgaaaagaa aaaaacgaac ttcaagaaaa
     3121 gaacacgccg cactgcaaaa taatggaggg ttaatcttat gcgtaatacc acacacaaac
     3181 aacaagaaat cgaaaatcaa aaagccaaaa ttcgttcaga acaggaaaaa atgcgtgcca
     3241 gcaaacgaag actgcagcaa ttgaagaacc agcagaggat tcaaaaagaa atcgaattag
     3301 atcgataaaa tcttcctaac tattgtatac gtataaactt atgttatact gtttttataa
     3361 gtttatacgt atacaaagga gggagaaaat gacaaaagca atggtataca agctgctaga
     3421 agaatttaat ggaactttgg ctttgaagga tgccaaaaaa gcgggcattt ctcctgtaac
     3481 gatcaaacgt atggtagatc gaggggagct ggatcgagag tatccaggtt ttttcacctt
     3541 acctggtcaa tttcctgatg aattgtttat ggcgcaaaaa aaatatgaac gcggcattat
     3601 ttcacatatt accgcgttag atttgtatga tttaacagat atgatcccta gacagatcga
     3661 tctgactgta ccttacggtt atcacgtttc tgaaaaagga ttgaaggaat tcgcagttga
     3721 gctacactat acaaaagcgg aatggtatga attaggtaag attgaaataa aaagtcgcta
     3781 tgggaatcct attatagctt atgatccaga aaaaaccttg tgtgacattt ggaatccctg
     3841 gtataacgtt gaggatgaaa tcaaagtaaa agcaatcaaa aactacatgg agtcggacag
     3901 aaaaaatctt agaaaattga atgagtatcg acgaattttg cctacagata aaacgatgcg
     3961 ctcttatatt atggctttaa attaaaggag aatacacatg acacctacgc aattaaaagc
     4021 aaaagtgaag aatatttcta gagaaaaaga agttgatccg cagcttgttt taagacattt
     4081 tatgatggaa aaattcttgg aaaaaatctc agagtctcct tatcgtgaaa attttgtgtt
     4141 aaaaggcggc ttcttaattg gctcaaagta tggcattgaa aatcgaacaa ctaaagacat
     4201 tgatacgact ttacgggaaa tgaaagtaac caaagaaaca ttgacaacgg ttctaaacga
     4261 tattttttca acgccgacga aagaaggcat tcaatttgag attcaaggaa tgaaagaaac
     4321 gagggaagct gattattatc cagggtttag cttacgtgta ctggcacatt tggaaaacat
     4381 gcggccagat tttaaggtag atgttactac aggtgactcg atctatcctg cgacgatcac
     4441 tcacagtcat aaattgatgt ttgaggatcg aacgattcct ttggaatcct atcctacaga
     4501 acaaattatt gctgaaaaat taagtgcaac ctttgatttt ttaacagata attcgagagg
     4561 aaaagatttt tatgatctct atactattcc taaaatggag aagatcgatg agaagacctt
     4621 gaaagatagt gtgagaaata catttacacg tcgaggaaaa actgatccgt taaagatata
     4681 ctatgaaaag gatatggatg tactgaaaaa tagtcacgag ctaaaagata cctggaagaa
     4741 atatcaaaaa agcaatcctt atgctgcgtc gatcacgtac gatgagacaa tggattcgat
     4801 tagtaatttg atggaaaaag tgattcacgt cgaagaaaaa gagcgaaaac tagcttatca
     4861 aagacaccaa cagatgaata gagaaatgga gagataaaga tgattaataa tgtgacatta
     4921 gtaggacgct taaccaaaga tcctgattta cgttataccg caagtggtac tgcagtagcg
     4981 acttttaccc tagccgtcaa ccgaaatttc acgaatcaaa acggcaattg agaagccgat
     5041 tttattaact gcgtgatttg gcgcaaacca gcagaaacaa tggcgacatt agctaaaaaa
     5101 ggtagcttga ttggtgtagt tgggcggatt caaacgcgga cttacgacaa ccagcaagga
     5161 caacgtgtat atgtgactga agttgtagca gataactttc aactattaga aagtaaagca
     5221 gcgacagaga gccgtgcgca cgctgatcaa tcgtccacca gtccaagcac cgccaccttt
     5281 gaacaacgga atacagcgac gcctaacaac aatggattga acgcttcaca gaatccattt
     5341 ggtggacaat caatcgatat tagcgatgat gatctaccgt tttagtttga gaaagaagga
     5401 gtaagcatgg caaaaaaatt agtctgacga tggatctatt gatacccgta attgcattta
     5461 tagacgggat ttctttgggc gtcactgaaa atcctttggt aaatagaatt gcgaaaggat
     5521 cactatttct tgtattcgtg tatcttgtct gttcttatct tttaaaaaga gcaggaaagg
     5581 ataattcctt ttggcgattg actaaagtca cagtagcagc gacagctgtt atacttacgg
     5641 ctattctacc gctttatatg tagagttaac gggaaatcgt tgaacttctt gcgaaatatt
     5701 gctataatga acatgaaaaa gaggagttgc gctaacaacc cctcattaac agcaccgatt
     5761 aaagaacggt ggcagaatat tagttatttg gaaataaccg ctgacctgct aaagtagacg
     5821 gttatttctt tttgtcattt ttaagcatat ttacaatcaa actaatcaaa gcaatggtaa
     5881 acataccaaa accaagaatg gtttgaattg tctcatatgc ggacaaaagg cttactcctt
     5941 tcatagattt tagcacttac attcataagc accaccacct ttccagaagt cgccaccgtc
     6001 ttcaaacttt actgtcttga ctattatagc atatctaaaa tcgaataaaa attctcaatt
     6061 ttagctgttt ttgctataat tttagcgagg tgatcgtgat ggaacaatat attggtttac
     6121 tcgataaatt agggtgatca agacacagcc attgcttgtg cgatttacat tacgcacgag
     6181 tcatgaacca atcaattgtg tagtggccaa tatagaaata gcggataaat tgctgatttt
     6241 ggaagaaggc aagtatacta tttcagtgac aggtcacttc aataaacgaa agcaactagt
     6301 cattgaatca atgcaaatta gaaaccctga tcattttacg cgatcgatga gcatataaaa
     6361 gaagagctgc agtcctaaat gaggattgta gctctttttg ttttacatag aaacagtaaa
     6421 aatgaaaagg cctgtttagg aaggcacaag cgccttctca attgaaagac ctttctctca
     6481 ctccaaccgc caccgccgtt cgagaagaaa ggaagtgaac gctacgcgtt cactaagata
     6541 aagggtcttg catagcaaga caaaatagaa aaaatttcct ctcaccaaaa ggttcgctcc
     6601 tgttcgttat ctctttattg atttgtcaga ccggcaacaa gttgcctttt atcaccaaaa
     6661 agcgacaagg acaagggtaa atggcacgct gcgcgccctt gtcttgtcgc ttttcgttga
     6721 ttcattctgg ccaacaaatc aataaagaaa gaactaacga aaggaagtaa aacctttcct
     6781 aagaggaggc agaatcaatc atgcgaaaga aatttgatta ttggggtgtt cccttcagcg
     6841 aattattccc aaactatcat gcaccacata ctgtcgaatg cgattgtgga gaaagagcga
     6901 aatgtatcaa aagctattgt ttgtatcaat gtccaacatg cgggaaaaaa tataccttgt
     6961 cttatggcga ttatatttta attgatgaga aaggaaagaa acgctgatga aaacacaaca
     7021 caaattattg actgtaaaag aagtcgttca aattttggtg gacaaagaag tcatttctga
     7081 aaaagtagca gaagttttac ttattccgcg gtactggacg atagaaaccc ctttagtaga
     7141 ggatgcaccg actatgctat ttgaagacct agtgaagatt gcaggatatg aactgcatta
     7201 tcaagaaaac ttggaaactt atgaaaacga atattggtta accaaattag agaactaaaa
     7261 aggagaaaaa aatatggctc aaaattacta tgacgaattt gtcaaattac ctttggataa
     7321 aatggcacaa aaaatggagg atatgacgtt cctatacaac gaaacacgtg ttccaaaaaa
     7381 acattataaa gaaaaattgt cggtagctgt ggaagaaatg attgaataag gtgtcgaaat
     7441 gaatcttatt gcgacgtact atcgaacact ggaagaactg aaaaagcaaa atgcaaaatg
     7501 gtttttccaa gccttactat gcttggaagt aggtgtcaaa ccaagcacta ttaaaccttc
     7561 tgaataccaa gctttagaac taacgtatgc gaaatttatc gaaacgaaaa aagccaagac
     7621 tgtttcaagt gaatggttag attattttga aaatatcaat aagtatggcg catattacac
     7681 catgaaaaag gaagacaacg aaaatgaata aggtgattta tcaactaaca acggattcca
     7741 tatcaacgat ttgaaagaaa aaaaggtcaa taatcattta taccgaaaag tgagaatgtg
     7801 tgaaacaagg acaaaaaacg cctagtaact aaggaaatgg ttcttatttc gcaccaatcg
     7861 ctccaaccgc ccccgccgct taggtggtgc gaaataaacg g
//
LOCUS       DACEXQ010000122         7875 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.122, whole genome shotgun sequence.
ACCESSION   DACEXQ010000122 DACEXQ010000000
VERSION     DACEXQ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.122"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..194)
                     /locus_tag="IXH04_001831"
     CDS             complement(<1..194)
                     /locus_tag="IXH04_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ1852429.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVRE"
     gene            complement(211..1608)
                     /gene="mnmE"
                     /locus_tag="IXH04_001832"
     CDS             complement(211..1608)
                     /gene="mnmE"
                     /locus_tag="IXH04_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAQ1852430.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            1952..2290
                     /locus_tag="IXH04_001833"
     CDS             1952..2290
                     /locus_tag="IXH04_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852431.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(2458..3216)
                     /locus_tag="IXH04_001834"
     CDS             complement(2458..3216)
                     /locus_tag="IXH04_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ1852432.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(3234..4052)
                     /locus_tag="IXH04_001835"
     CDS             complement(3234..4052)
                     /locus_tag="IXH04_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAQ1852433.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(4049..4405)
                     /gene="rnpA"
                     /locus_tag="IXH04_001836"
     CDS             complement(4049..4405)
                     /gene="rnpA"
                     /locus_tag="IXH04_001836"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAQ1852434.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(4523..4657)
                     /gene="rpmH"
                     /locus_tag="IXH04_001837"
     CDS             complement(4523..4657)
                     /gene="rpmH"
                     /locus_tag="IXH04_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAQ1852435.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            5180..6514
                     /gene="dnaA"
                     /locus_tag="IXH04_001838"
     CDS             5180..6514
                     /gene="dnaA"
                     /locus_tag="IXH04_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAQ1852436.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            6712..7842
                     /gene="dnaN"
                     /locus_tag="IXH04_001839"
     CDS             6712..7842
                     /gene="dnaN"
                     /locus_tag="IXH04_001839"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAQ1852437.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
BASE COUNT         2313 a         1621 c         1409 g         2532 t
ORIGIN      
        1 atttctcgaa cgacaacgcc tttagctggt ccgcctacag aaggattaca tggcatgaat
       61 gctaccatgt ccaaatttat tgtcagtaat aatgtttttg atcccatacg tgcagcagca
      121 agtgcagctt ctgaaccggc gtgtcctgcg ccaacaacga tcacgtcata cgcatctgct
      181 tcataatgtt ccaatcgttt gttcctcctt ttattttcct aaacaaaatt gactgaataa
      241 ttgagtgatt agctcatctt gtacactgtc gccgacaact tctcctagat agtcccagca
      301 acgcgtcata tctatttgaa cgagatccac aggcattcct gaatcaatac ctgcaatgac
      361 ttcttcaagt gagagggaag ctttttctaa caacgcgata tggcgggtat tagaaacata
      421 agtagcgtct ctttctcctg tttctccgct gaagaataaa tcagaaatag ctgattccaa
      481 acgatctagt ccgtcgtttt tagctacgga aattgaaaaa actggttcat tttcgatcaa
      541 tttcttcaat ttttcttgtt ccagctgtgc tggcaaatct gttttattta gaagaatgat
      601 ccgtttcaat ccacttgtcg cttccaataa ttgttcatct tctgctgtca gtggttcgct
      661 ttggtttaga acaagtaaga tcaagtctgc ttctgctaat gcttttcggc ttctttcgac
      721 cccgatccgt tccaccacat cttctgtttc ccgaattcca gctgtatcga ttagcttgag
      781 cggtaccccc cgaacattga catactcttc gattacatct cgagtcgttc ctgcaatatc
      841 cgtaacgatt gctttttctt ctcttaacaa atggtttaac aaacttgatt tgccgacatt
      901 tggccggccg atgattgccg tacttaaccc ttcccgcaga accttccctt gcttggatgt
      961 agcaagtagc gcttggattc tttgttggat catctgcgct ttttcaagca ataacttcgt
     1021 agtcaattct tcgacatcat catattctgg ataatcaata tttacttcta cttgtgctaa
     1081 tgtctctaaa atctcttgtc tcaatgagcg gatcaaagca gacagattgc cgtctaactg
     1141 attaagagct agtcccattg ctttgtctgt ttttgcccga atcaaatcca tcacagcttc
     1201 tgcctgtgaa aggtcaacac gaccattcaa gaatgctcgt tttgtgaatt cgcctggctc
     1261 agctaatctt gcaccttctc ttaacaatag ctgcaaaatt tgattgacta caacgatccc
     1321 accgtgacaa ttgatttcta ctacatcttc ccgcgtaaat gtctttggtg ctctcatcac
     1381 ggagaccatg acttcatcga caagctgatt gctcttggga tcaacgatat gtccataatg
     1441 gatcgtatga cttggtactt ctgataatcg tttgtttcca gattgataaa ctttatctgc
     1501 tagttgcaca gcttgatcgc cacttaaacg aacgatactg attgcacctt ctccaggtgg
     1561 ggtagaaatt gccgcaatcg tatcaaattc taaagtaatt tcagccattt tttcacttcc
     1621 tttttcattt tgcgcacaaa aaaagtgccc actccaccct ttatgcacaa taaacggtga
     1681 acttcgcact ttgactgctt gttcttgatt aaattctggt catagattat cacacttgct
     1741 ataaggaatc aagtgtttaa aacaattaga tgaagttgag cctgttctgc ttccattacc
     1801 ttatatttaa ttgtccttgg atgtctcttt gcgttctttg gtttattcga agagaagata
     1861 ctgtataaac aaggctttat ctttcttaaa aaaaacggta cactaaatgg tagcgctatc
     1921 taaactatac acttttgaaa ggagaaaata tgtgagcccg ttcagatctg ctgcattgga
     1981 agaagaaagt gaccctcgga tcaacgatgt cgttttatct acagggaata tcaaccagaa
     2041 atatatcgtc cgcgatatcg tttttgttgt ggaatgctta gaagctgatt tgtttgaccc
     2101 atcttttccc aaagatgacc ttttcttaac aacgaagagg aaattaaaga cgaaggtact
     2161 tgaatatggt gcgaatgcag taatcaactg tcattttgag tatcagcaaa aaaaacgcga
     2221 cggaaagtat tttctggaac tttttgctta tggaacagtg atccaattta tccaaacaac
     2281 gattggttaa atgaaacaga caaaaaaagc ggatcaaaac tgaagacagt ttcgatccgc
     2341 ttttttctat caaagttagt gactacttca tcgtatactc tgataagaat gatttctagt
     2401 aaaatatact tggtttgaaa aactttgtca acgtgattta ttcaaaaagt aatggaatca
     2461 ataatatttt ttagcaggtt ctactactaa ataacgataa ggttcctcac cttccgaatg
     2521 tgtttttaca tgttcatctt tgcttaatgc agcatggatt tgtttacgtt caaatgcagg
     2581 cattggttct aagaaaacag gctgccctgt ccgttttact ttttcagccg tgcgatcagc
     2641 taaacgctga atgatctctt ggcgcttttc acggtaattg cctacattga ctactacaga
     2701 cagcttattt cttgctaaac gatggatgaa gacttgtgcc agatattgca atgcattcag
     2761 tgtttttcca tgtttcccaa tcagtatgcc ttgcttttgt gtttctaaat ggaaaacaac
     2821 catattattt gattctcttt ccattttgac aagagctgga gcatcaagct gttttgaaat
     2881 ttcagtaaga taaactgcca attccgttaa agctgcttca tcacctagct cacttacttc
     2941 ttctgttttt tcttcaagaa tcgtttcgtt gactacctcg atctcagctg ttggttcttc
     3001 ttccaaaatt tcttctacag tctgttctac tgtttcagaa actttttcgc tgatacttgg
     3061 ctggatcgaa agccgtgcat tctttcgacc cattcctagg aaaccttttt ttccttcttc
     3121 cagtatttcg atttcgactt ggtctttggc caaacccaaa tcagccagac ctagttggac
     3181 agcttcgtct attgttgcag cttcataaat cggcattagc attacctccc ctattatttc
     3241 cgttttttct taggattcat tgctttctta agagcacgtt ctctttcgcg gattctccgt
     3301 gcttcttctt cacgctcttt tcgaattttg aaagggttgt tgatcagcag cgtttgtaca
     3361 acttggaagg cattggaaac tacccagtat aatgaaagac cgctggctaa attgacaccc
     3421 atcactaaaa tcattacagg catcacgaaa ttcatgatct tcattgatgc attcgattca
     3481 atttgactca tgctagataa atacgtactt gcaaaagtaa agattgctgc taaaatcggc
     3541 aaaataaatg ttggatccgc gttccctaag ttcaaccata agaaatgccc ttgtttcaac
     3601 tctggtacac gtgaaataga ttgccataac gccatcaaga ttggcatttg tactaataaa
     3661 ggaagacaac cagcatatgg attgacgcca ttttctgcat ataaacgctg ctgctcttct
     3721 cttaacttac tttgtgtttc cggatctctt gaactatatt gttgctgcag tgcttttaat
     3781 ttcggttgta attcttgcgt tttacgcatg ctctttgttt ggaaatgcat caacggcaat
     3841 aggatcacgc ggatgatcag tgtaaataaa atgatcccta tccctgcatt accaaatgac
     3901 aacgccttga tcgcctcagc aaagtaataa acaatataac gatcccaaat tcctgtactc
     3961 tgtgcgctga cttccccagt tccgcaaccg gataagacga tgaccaaact aacgataccg
     4021 gccatcaaaa gtaaacgttt gtatttcttc acttactctt ttcctctctg tcgattattt
     4081 ttgcaagttt taagacgtgg actaaattgg agcgaacctc atcataagtc aaccccttca
     4141 cacttggtct agcaatcaca ataaaatcca actccggatc aatttcttct tttatagaat
     4201 aaactgctgc tcggattttt cgtttgactt cattgcggtt taccgcgttg ccgacctttt
     4261 ttcctacaga taaaccaact cgaaaatgag gctgtcccgg tttttccaaa cggtaaacga
     4321 cgaatttgcg gttagcaaaa gaatctcccg tatggaacac ggcttgaaac tctcgttctt
     4381 tttttactcg atacgctttt ctcattagtt ttctttcctt ttctatctct cacttacgct
     4441 cataatcata ccataattac gattgtcctt tcatcatttc cctaaaaaag agtataaaaa
     4501 aaaccactga aaccccagtg gcctaagctg aaagaacttt tcttcctttg cgacgacggc
     4561 tagctaacac acgacgtcca tttttagtac tcatacgttt acggaatccg tgaacttttt
     4621 gacgtttacg tttatttggt tggtatgttc ttttcatata ttccacctcc aaaatgttct
     4681 tatctatttt ataaacacag acatactttg atagtataac gacaaagtag tgattttgtc
     4741 aatataaaat agaagatcga ttgttaaaac ttatccacaa gattattttt ttctatggga
     4801 atcctatgtg gaaaaagtta tgcacaggga ttatccacag ataataaaag gctggtttta
     4861 cacagaaaac acacgtgtaa aaaaagtaat ccacaatcag gcgatttcct gtgtattccg
     4921 tctcctaact attggtatat ctgtattttt tatccacaga cgcaagagga aatctattgg
     4981 tttttacgag ttttccacct gtgcattagt ggtgtggaaa actttttttg acaactgtgt
     5041 ttttctcttt tttctttttg tcgattgtgt ggataattgt aattaaaaaa tttttttctt
     5101 cggtttctgt ggaaaactat tgaaaaaaat gctaaattag tactagttat tcacttttta
     5161 atgaaggagg aggccttata tggtatccct cgatgcttta tggaatgaat taaaagcaac
     5221 ataccaaaag gatttatcgc ctgctagcta taatacatgg atcgaaacag cgaaccctcg
     5281 aacccttgat caaaaccagc tcgttgttga ggttcctagc aagattcata aagaatattg
     5341 ggaaaaaaac ctggcgacaa aaatcgttga agtcggttat atgttatcag gaaatgagat
     5401 tatccctcgc tttattactg gcgaagaagc agagcaagaa gaagttatag aagaaaaaaa
     5461 tccaaaagtt gtggcaccta gtccgctaaa aaaagccatg ctgaacccta aatatacctt
     5521 tgatacgttt gtcattggta aaggaaacca gatggctcat gcagcagcgc tcgttgtagc
     5581 agaagatcca ggttctattt ataacccgct cttcttttat ggaggcgtgg gacttggtaa
     5641 gactcacttg atgcatgcaa ttggtcatca aatgctgcaa agtcagccta atgccaaagt
     5701 aaaatatgta agcagtgaaa cttttgctaa tgatttcatc aattcgatcc aaaacaaaac
     5761 agcagaagag ttccgtcaag aatatcgaaa tgttgattta ctgctagtgg atgacattca
     5821 attttttgca gaaaaagaag ctacgcaaga agagtttttc catactttca atgccttgta
     5881 caatgaaggg aaacaaatcg tactaaccag tgatcggttg ccaaacgaga ttccgaaact
     5941 gcaagaacgc cttgtttctc gctttgcatg ggggctgtct gttgatatta caccgccgga
     6001 tcttgaaaca agaacagcga ttttacgtaa aaaagcggcg gctgaacgtc tagagatccc
     6061 agatgacaca ctaagctaca tcgcaggaca aattgattct aacattcgtg aattagaagg
     6121 tgcgcttgtt cgagtgcaag cttttgctgc gatgaatagt gaagatattt cgactagtct
     6181 agctgcagac gcactgaaaa cgttaaaatc aggaaaagga catcctcaat tgtcaatcct
     6241 gcagatccaa gaagaagtgg caaagtatta tcacatccaa ttgaaagatc tcaaaggaaa
     6301 gaaacgcgtc aaatctattg ttgttcctag acaaatcgct atgtatttgg cacgtgagct
     6361 cacagataat tctttaccga aaatcggagc tgaattcggt ggcaaggatc atacaacggt
     6421 tatccatgct catgaaaaaa tccagcagct gatggattct agtgtcagta tgcaaaatga
     6481 agtatcagaa atcaaaaact ttttactaaa ttaatttgtc gattctatca actgaaaata
     6541 gcatgtggat aaaagaaaaa aacgttcaaa agttatacac actttttcca cagctgtttt
     6601 ttcgtttctc tcttacgttt tttgagtttt ccacagaatc aacatgccct attactttta
     6661 ttattctttt taactaataa taaataaaca aacattcaaa ggagatacat catgaaagtt
     6721 actttaaacc gagctagctt tatgcaggaa ttgcaaactg ttcaacgagc tatttcaagc
     6781 aaaaccacga tccctatttt gacaggtgta aaaatcacac tgacacaaga aggtttgact
     6841 ttgacgggga gcaacgctga tatatcaatt gaaacttttt tgtctgttga aaacgaaaaa
     6901 gcaaatatgc aaatcgaatc tactggttcc attgttttac aagcacgttt ctttagcgaa
     6961 atcattcgga gacttcctga agaaacattt actttagaag ttttagaaaa taaacaagta
     7021 gcgatcactt ctggaaaagc gaattttatc gtaaatggat tagatgcaga taactatcct
     7081 catcttcctg ttgtcgaaag ccataaccag atgaaattac ctgtacacgt attgactaaa
     7141 ctaatcaacg aaacagtttt tgctgtctca caacatgaga gtcgtccaat cttgacaggt
     7201 gtgcatttca ttttatctga taattcttta ttagctgtag ctactgattc tcaccgtcta
     7261 agtcaacgcg tgattccagt agaacaagcg gctgatcatt ttgatattgt tattcctgga
     7321 aaaagtttga tcgaattatc tcgctcatta acaaatgaag aagaaatcgt cgaaatcagc
     7381 attatggaaa accaagtgtt gttcaaaaca gaaacgatgt atttctattc ccgtttgcta
     7441 gaaggaaact atccagatac caatcgtttg attccttcaa gttttaatac agaggttgaa
     7501 ttttctgttc ctagcttttt agcggcgatc gaacgggctt ctttactttc tcatgaaggc
     7561 cgtaacaaca tcgttcgttt atctattcgt ccagatgcgg ttgttttata cggaaattcg
     7621 ccagaaatcg ggaaagtaga agaaagcttg agttatacag caagcagtgg cgatccatta
     7681 gatatttctt tcaatccgga ttatatgaaa gcagcgttgc gtgcatttgg agatatgagc
     7741 atcaaagtga aatttatctc tgctattcgt ccatttacat tggaaccaac ggaagatggc
     7801 gttcaattta ttcagctgat tactcctgta cgtacaaatt aacaatgatt ttcaatgttc
     7861 ttgaaaattt aattg
//
LOCUS       DACEXQ010000123         7827 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.123, whole genome shotgun sequence.
ACCESSION   DACEXQ010000123 DACEXQ010000000
VERSION     DACEXQ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7827
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.123"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            94..1398
                     /locus_tag="IXH04_001840"
     CDS             94..1398
                     /locus_tag="IXH04_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAQ1852438.1"
                     /translation="MLKTLNRIKIGFIPLSLYIILVLAYLAMTISNIVPENDMLVSLG
                     IMTVFAYLLEEIGKTIPILKDLGGKVLVVTFLPAFLVYKHWLPQSTVTVVSDFMNNNN
                     FLMFFITLLVVGSIASMNRQTLVKATSRIIIILILCDVIGSLIGTGVGILLGLSAWNA
                     YFFIVAPIMAGGVGEGAMPLSIGYAAILSGMGQNDIFGKILPCVMLGSLVAVLLSGML
                     KKIGEKYPQLNGQGNLIDGDDEHLENLKGSAKPVNIEKTLLAGVVAITIYFVAVYVEH
                     LLKSLLGFSIPSPVILLVSLMGAKMLGFIPKNIEEGGNSLYGFTVRGITPPLLFGVGV
                     AKTDWLELIEVFKNPATIVTIIVTVVAIVATAFIAAKFLKMYPVDTAIAVSCCSGQGG
                     TGALAILAAGDRMELMPFAQVAVRLGGAMTVTFAIFLMGLLS"
     gene            1424..2437
                     /gene="citC"
                     /locus_tag="IXH04_001841"
     CDS             1424..2437
                     /gene="citC"
                     /locus_tag="IXH04_001841"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HAQ1852439.1"
                     /translation="MFDQTDIQRLFLKNNYIKDQWENLLLDSGIQKKQIEDFQHLDQT
                     LGIYHKEKLVGTVSYQNNVIKYIAVSEKYKDGGHTFNVLVSAIIQAMSQQEIFHYIVF
                     TKPRYQKSFNFLGFETIVSTPKIAVLEMGDSSIKQYLERIPKVNGEKKAAIVMNANPF
                     TEGHYYLVKQAANNNDWVYVFVLEKEQNLFSTKDRLKLVQQGLQKLKNVIVLSGGEYI
                     ISPATFPTYFLREDDEIAKEQMVVDATLFKERIATYLDISARYVGEEPLSPMTRSYND
                     ILETILPPEIKVHVLARKKTEQHQVISASQVRKAYLAGQLEKIKYMVPETTYQYLKNK
                     RER"
     gene            2441..2734
                     /gene="citD"
                     /locus_tag="IXH04_001842"
     CDS             2441..2734
                     /gene="citD"
                     /locus_tag="IXH04_001842"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HAQ1852440.1"
                     /translation="MEINKSAIAGTLESSDVQIMISPNTEGIEIDLDSQVIEQFGKQI
                     KKVIYDTLAVYQIKNVKLKVVDKGALDCVIVARLKTAIHRSLGIQHQETPWEV"
     gene            2739..3644
                     /gene="citE"
                     /locus_tag="IXH04_001843"
     CDS             2739..3644
                     /gene="citE"
                     /locus_tag="IXH04_001843"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HAQ1852441.1"
                     /translation="MMKERLRRTMMFVPGNNPAMIKDAGIYGADSIMFDLEDAVSMAE
                     KDSARMLVAEALKTVDYGDTELIVRINDLHSEFGRADIFAMVKAGAHVLRLPKTETSE
                     DITEVEKVIIEAENYHNVPIGRTLMMAAIEGASGVLNAPAIAKASDRLIGIAIGAEDY
                     VTNMKTKRYPDGNELFFARSMIVHAARAAGISAFDTVYSDVDDMEGFLNEVRLIHQLG
                     FDGKSCINPRQIPVVNTVYQPSIKEIKRSLDIISAIEEAKEKGSGVISLNGKMIDKPI
                     VERAQRTLMLAKASRLIDEEGNIHA"
     gene            3637..5196
                     /gene="citF"
                     /locus_tag="IXH04_001844"
     CDS             3637..5196
                     /gene="citF"
                     /locus_tag="IXH04_001844"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HAQ1852442.1"
                     /translation="MHNKQRRSIPSSVLTKYKKEFQSVSLSPQTIQRCAPKVTTVTNQ
                     TNKLMHSIKEAIEKSGLKDGMTISFHHHLREGDFVFNQVMAVIREMGIKELTLAPSSL
                     TNTMNEMVIECIKDGTVTHITSSGMRGSLGEFISHGGMDNPVILRSHGNRPRAIESGE
                     LKIDVAFLGVPTSDDYGNANATVGKSVFGSLGYAKVDAQYADKVILLTDHLVPYPNTP
                     ISIPQTQVDYVVEVEAIGDPNKIGAGATRFTKNPKELKIAEMVKDVIVHSPYFKDGFS
                     FQTGTGGAALAVTRYLASEMEEKQIKASFALGGITKPTVDLLEKGLVEKVLDVQDFDK
                     GAAEGMARLENQQEIDASFYADPSNKGAVVNQLDVAILSALEIDTNFNVNVMTGSDGV
                     LRGAIGGHQDAATAKLTIISAPLVRGRIPTVVNDVTTLITPGKSIDVLVTEVGVAINP
                     QRKDLIAIFERIPQIPVFTIEELQQKAEKIVGIPEPLQFTDRIVAYVEYRDGSILDVV
                     RQVKEFEEERS"
     gene            5193..5750
                     /gene="citX"
                     /locus_tag="IXH04_001845"
     CDS             5193..5750
                     /gene="citX"
                     /locus_tag="IXH04_001845"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HAQ1852443.1"
                     /translation="MMFDTGEIQEIEDILIHKEQRANLQSNLLSKYSNSSLMVFTLNI
                     PGPIKNNPMIEQVFRQGQQSIQQMFLKKKVEMTYTKEINLPSGLDWYVIVTEAPLNLK
                     KWLVTLEEQDALGRLFDLDVLFKKGEQITIVSRQALNHGERPCFICGKSAKVCARNRT
                     HTVKEMQKKIEQIVADSLKGALKHA"
     gene            5743..6606
                     /gene="citG"
                     /locus_tag="IXH04_001846"
     CDS             5743..6606
                     /gene="citG"
                     /locus_tag="IXH04_001846"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HAQ1852444.1"
                     /translation="MHDNIQKIAQEAQLAMLYEVTCINKPGLVDPVDPGSHQDMDIFT
                     FLQSSVVLTPYFEEFIQTGLDRQHQPIEETFMAIRQIGLEAETAMFSVTNGINTHKGA
                     IFSLGIFLAICGRLTIWKVPCKKSFFQKEIQKMTKNLLNDFGKIDQTKPKAWTWGEYL
                     FVNHGLTGIRGEAKRGYPCVFDRGLPYYQAYQGSQQDKMIDTLLFLSMEVEDTNLIKR
                     SNNRHVFEEYQLLIHPYFELGGVKTIQGKKYLDYLNQQFKKKNWSIGGSADLLILTIF
                     LDKIMDKGWLC"
     gene            6840..7249
                     /gene="tnpA"
                     /locus_tag="IXH04_001847"
                     /pseudo
     CDS             6840..7249
                     /gene="tnpA"
                     /locus_tag="IXH04_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334252.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT         2715 a         1220 c         1584 g         2308 t
ORIGIN      
        1 tccctaatgt ttttgatgaa ggagtcgcaa acgtcgtttc acaggcagtt ttggattatt
       61 tacaaaaact aaaagaaaac gaggataata tacatgctga aaacattaaa tagaattaaa
      121 attggtttta ttcctttatc tctttatatc attttggtac tcgcgtatct tgcaatgacg
      181 ataagcaata tcgttccaga aaatgatatg ttagtttctt tgggtattat gactgttttt
      241 gcttatttat tagaagaaat tggtaaaact attcctattt taaaagattt aggtggtaaa
      301 gtattagttg ttacattttt gcctgcattt ctagtctata aacattggct acctcaaagt
      361 actgtaactg tggtttctga ttttatgaat aataacaatt tcttgatgtt ctttatcaca
      421 ctactagttg tcggcagtat tgcttcaatg aatcggcaaa cattggttaa agcaacttca
      481 cgtattatca ttattttaat tttatgcgat gtgattggtt ctttgatagg aacaggtgtg
      541 ggaatactcc ttgggctatc tgcttggaat gcgtatttct ttatcgttgc gccgattatg
      601 gctggtggtg ttggggaagg agcgatgcca ttatcgattg gttatgcagc aatattatct
      661 ggtatgggac aaaatgatat ctttgggaag attctaccgt gtgtgatgtt gggaagttta
      721 gtcgcagttc tactgtctgg tatgctcaaa aaaattggag agaaataccc acaattaaat
      781 ggtcagggta atttgatcga tggggatgat gaacatttag agaacttgaa gggttcagct
      841 aaaccagtca atattgaaaa aacattgtta gcaggcgtgg tagcaataac gatttatttt
      901 gttgctgtct atgttgagca cttattaaaa tcactccttg gcttttctat tccttcacct
      961 gtgatcttat tagtctctct tatgggggct aaaatgcttg gttttatccc taaaaatatc
     1021 gaagaaggtg gaaattcgtt gtatggattc actgttcgtg ggatcacacc tcccttactt
     1081 tttggcgtag gggttgcaaa aacagattgg ttagagctga tcgaagtatt taaaaatcct
     1141 gcaacgatag taacaatcat tgtgacggta gtagctattg tcgctacagc ttttatcgcc
     1201 gcaaaatttt taaaaatgta cccagttgat actgccatcg cagtttcatg ttgtagcgga
     1261 caaggcggta caggtgcttt agctatctta gctgcgggtg atcgcatgga attaatgcca
     1321 tttgcacaag ttgctgttcg actagggggt gctatgaccg taacgttcgc tatcttttta
     1381 atgggtctgc tatcataaga acataaagaa aggacgtgac catgtgtttg atcaaacaga
     1441 tatccaacga ttatttttaa aaaataacta tatcaaagac caatgggaaa atcttttgct
     1501 tgattcagga atacaaaaga agcaaatcga agattttcaa catttagatc aaacattggg
     1561 tatataccat aaagaaaagt tagtaggaac agtatcttat caaaacaacg tgattaaata
     1621 tatcgccgtg tctgaaaaat ataaagatgg tggacataca tttaatgtac tagtctctgc
     1681 aataattcag gcaatgagtc aacaagagat ttttcattat attgttttca cgaaaccgcg
     1741 ctatcaaaaa agttttaact ttttaggttt tgaaacaatc gtatctaccc caaaaatagc
     1801 tgtattagaa atgggagata gttctataaa acagtaccta gagaggattc ctaaagtgaa
     1861 tggtgaaaaa aaagcagcaa ttgtcatgaa tgcgaaccca tttacagaag ggcattatta
     1921 tttagtaaaa caagctgcca ataacaacga ttgggtatat gtatttgtac tcgaaaaaga
     1981 acaaaatctt ttttcgacga aggaccgact caaattagtt caacaaggat tacaaaaact
     2041 gaaaaatgtc attgttctat caggtggcga gtatattatt agccctgcaa cgtttcccac
     2101 ctatttttta cgagaagatg atgaaatagc aaaggagcaa atggtcgtag atgctacttt
     2161 attcaaagaa agaattgcca cttatttaga tatctctgct agatatgtgg gagaagaacc
     2221 actttcccca atgactcgtt catataacga catactggaa acgattttac cccctgaaat
     2281 aaaagttcat gtccttgcaa ggaagaaaac ggaacaacac caagttattt ctgcttctca
     2341 agttcgtaaa gcgtatcttg ctgggcaatt ggaaaaaata aaatatatgg ttccagaaac
     2401 aacctatcaa tacttaaaaa ataaaaggga gcgttaagta atggaaataa ataaatcagc
     2461 gattgctgga acattagagt caagtgatgt ccaaatcatg atttcaccga atacagaagg
     2521 aatagaaatc gatttagaca gtcaagtgat tgagcaattt ggaaaacaaa tcaaaaaagt
     2581 tatctatgac acactagcag tttatcagat taaaaacgta aaactcaagg tagtagataa
     2641 aggggcatta gattgcgtta ttgttgcacg attaaaaaca gcgatccatc gttccttagg
     2701 tatccaacac caagaaacac cgtgggaggt ttaattagat gatgaaagaa cgtttgagaa
     2761 gaacaatgat gtttgttcca ggaaataatc cagcgatgat caaagatgca ggtatttatg
     2821 gtgctgactc gattatgttc gacctggaag atgcagtgtc tatggcagaa aaagacagcg
     2881 caagaatgct tgtggctgag gcattgaaaa cagtggatta tggtgacaca gaattaattg
     2941 ttcgaataaa tgacctgcac tcagaatttg gacgtgcaga catttttgca atggttaaag
     3001 ccggcgcgca tgtattacgt ttacctaaaa cagaaacctc tgaagatata acagaagtag
     3061 aaaaagtgat tatagaagca gaaaactatc ataatgttcc gatagggcga acattgatga
     3121 tggcagccat tgaaggtgct tctggtgtat tgaacgcacc tgcgatcgcc aaagcatctg
     3181 atcgattgat tggaatcgcc attggggcag aagactatgt gacaaatatg aaaaccaaac
     3241 gatatccaga tggaaacgaa ttgttttttg ctcgcagtat gatcgttcat gctgcacgtg
     3301 cagcaggcat ttccgcattt gacactgttt actctgatgt ggatgatatg gaaggatttt
     3361 taaacgaagt acgtttaatc catcaattag gttttgatgg aaaatcttgt atcaatccac
     3421 ggcaaattcc agtagtcaat actgtttatc agccgtcaat caaagaaatc aaacgatcac
     3481 tagacatcat ctcggcaatc gaagaagcaa aagaaaaagg ttctggcgta atttctttaa
     3541 atggaaaaat gatcgataaa ccaattgtag aacgtgcaca acggacattg atgttagcaa
     3601 aagcatcacg attgattgat gaggagggaa atatccatgc ataataaaca aagacgtagc
     3661 attccatcaa gtgttttaac aaagtataaa aaagaattcc aaagtgtttc attatcgcca
     3721 caaacgattc aacgttgtgc accaaaagta acgactgtaa ctaatcagac aaataaattg
     3781 atgcactcga tcaaagaagc tatagaaaaa tcgggcttaa aagatggaat gacgatttca
     3841 tttcatcatc atttgagaga aggtgatttt gtttttaacc aagtgatggc cgtgatccgt
     3901 gaaatgggca tcaaagaatt gaccttagca ccaagttctt taacgaacac tatgaatgag
     3961 atggttattg aatgcatcaa ggacggtaca gtaactcata tcacaagcag tgggatgcgc
     4021 ggcagcttag gtgaatttat ttctcatgga ggaatggaca atcctgtgat tttacgttct
     4081 catggtaatc gtccacgtgc aattgaaagt ggtgaattga aaatcgatgt tgcatttcta
     4141 ggagtaccaa catctgatga ttatggaaat gctaatgcga ctgttggaaa atccgttttt
     4201 ggttcattag ggtatgcaaa agtagatgca caatatgcag ataaagtcat tcttctaact
     4261 gatcatttag ttccttaccc taatacgccc attagtattc cgcaaacaca agtcgactat
     4321 gtcgtggaag tagaagcaat tggtgatccg aataaaatcg gtgctggtgc tacacggttt
     4381 actaaaaacc caaaagaatt aaaaattgct gaaatggtca aagatgtcat tgttcactca
     4441 ccttatttca aagatggctt ttcttttcaa acaggaacgg gtggtgcagc tttagcagta
     4501 acgcgttact tagctagtga aatggaagaa aaacagatca aagcttcttt tgcattaggt
     4561 ggcatcacta aaccaacggt tgatttattg gaaaaaggtc ttgttgaaaa agtgttagat
     4621 gtccaagact ttgacaaagg agcagctgaa ggaatggcgc ggcttgagaa ccaacaggag
     4681 atcgatgctt ccttttatgc tgatccaagt aataaaggag cggttgttaa ccaattagat
     4741 gtcgctattt tatctgcatt agaaattgac acaaacttca atgtaaatgt tatgactggt
     4801 tcagatggtg tgctaagagg tgcgattggt ggacatcaag atgcagcaac ggctaaactt
     4861 actatcatct cagcgccgtt agtacgtggc cgaataccta cagtagtaaa tgatgttact
     4921 acattgatca caccagggaa aagtatcgat gtattagtca cagaagtagg ggttgctatc
     4981 aatccccaac gtaaagatct aatagctatt tttgaaagaa ttccacaaat tcctgttttt
     5041 acaatagaag aattacagca aaaagcagaa aaaatagtag gcatcccgga acctttgcaa
     5101 ttcacagatc ggatagttgc ctatgtagaa taccgagatg gttcaatttt agatgtcgtt
     5161 cgtcaggtga aagaatttga agaagagaga tcatgatgtt tgatactgga gaaattcaag
     5221 aaatcgagga tattttgatc cataaagaac aacgtgctaa cttgcaaagc aacctgcttt
     5281 ccaagtattc aaatagttca ttgatggtct ttacattgaa tattcctggg ccgatcaaaa
     5341 ataatccaat gattgagcag gtatttcgtc agggacagca gtcaattcag caaatgtttt
     5401 taaaaaaaaa ggtggaaatg acgtacacaa aagaaatcaa tttgccaagt ggattggact
     5461 ggtacgtaat agtaacagaa gcacctttga atctcaaaaa atggttggtc acgttagaag
     5521 agcaagacgc attaggtaga ctttttgatt tagatgtctt gttcaaaaaa ggagagcaga
     5581 taacaattgt cagtcgccaa gctcttaatc atggggaacg tccttgtttt atttgtggga
     5641 aatcagcaaa ggtatgtgca cgtaatcgta cacatactgt gaaagaaatg caaaagaaaa
     5701 tcgaacaaat agtagccgat tccttaaaag gagcattgaa acatgcatga taatattcaa
     5761 aaaatagcac aagaagcaca attagcgatg ctttatgaag ttacatgtat caataaacca
     5821 ggattggttg atccagtaga ccccgggtct catcaagata tggacatttt tacttttcta
     5881 caaagtagtg tagttttgac tccctatttt gaagaattta tccaaacagg attggaccga
     5941 caacatcaac caattgaaga aacctttatg gcgattcgac agattggttt agaggctgaa
     6001 actgcgatgt tctcagtgac aaatggtata aatacacata aaggtgctat cttttcattg
     6061 gggatttttc ttgctatttg tggccggtta actatttgga aagttccttg taaaaaaagt
     6121 ttctttcaaa aagaaattca aaaaatgacg aaaaatctac ttaacgattt tgggaagatt
     6181 gatcaaacaa aaccaaaagc atggacttgg ggagaatatc tatttgttaa ccatggattg
     6241 acagggatac gaggggaagc caaaagaggt tatccttgtg tttttgaccg aggattgcct
     6301 tattatcaag catatcaagg gtcgcaacaa gataagatga tagacacttt actttttcta
     6361 tcaatggaag tagaagatac caatttgatc aaacgaagta ataatagaca tgtttttgaa
     6421 gagtatcaat tacttattca tccttatttt gaattagggg gtgtaaaaac catacaaggt
     6481 aaaaaatatt tggattacct aaatcagcaa tttaaaaaga aaaattggag tattggtgga
     6541 agtgcagact tattgatcct tacaatcttt ttagataaaa tcatggacaa aggatggtta
     6601 tgttgatact ataatacatt attttgtatt agtaagctaa taactaaaaa atggttcaat
     6661 acaaatattt tataaaaaat ggtagtgcct atcctttcag attgtagaca aagaaaaatt
     6721 tctttgtcta caattttttt gttaatgact tcagtcagtt tcgtgcgcaa agcgtgcgaa
     6781 acaagaaaac catgcgtttc tacacgtgga agaactagca tatcgaaagg agttttttca
     6841 tgtcaaatga cgataaaagt ttagcccata ctcgttggaa ttttaagtat catattgtat
     6901 ttacaccaaa atatcgacga aaagtaatct ataggcaatt acgaaaagat attggaaaaa
     6961 tacttaggaa gttgtgcaaa ataaaaaacg tggaaattat agaagcacat gcaacgccag
     7021 atcatattca catgcttgtt caagtaccat caaaaataag tatatccagt tttatagtta
     7081 tctgaaagat cgtagtgctg tgctaatcca cgagcagcac gtaaatcaca aatatagata
     7141 cggaaataaa tcattttggt cggaggaata ttatgtgagt acagtcgaat tgaatcaaaa
     7201 gacaattgaa aaatacatcc gtgaacaaga agctgatgat cgcatttgag atagtataag
     7261 taaaagagag tagatgttgc aataacaaaa gggtaaaaat ggactacctc cagtaaaata
     7321 ccgaaaaata gtttaacttt tggagataag tccatttaag ggtctataga atgacaattt
     7381 ttttagggga ttttactatt attacttcaa gacccgcttg gattcgcaaa gtgagtgaac
     7441 tcttaaaact acctgatagt aggttgtttt aagagttatc tgaaagtaaa aattgcattt
     7501 atatttttga taaatacgta aatttatata tacgtattta tcaaagtttt aggccaaagg
     7561 aatattgtgt aaatacacta ggattgaatg aatagaccag aagaaattgc gtccaagaac
     7621 aaaagaagca tgatagtgct ctatataaat tgggcattca agaatctgaa atttaaaggt
     7681 agaattttag ttagactttt agattttaaa cacaaaaata accatacaag caacaagaag
     7741 caaagtgcta tattataaat tgtccgtccg tttacttaca taagatcatt ggggctattt
     7801 ttaaaagaga ataatagata ataaatg
//
LOCUS       DACEXQ010000124         7809 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.124, whole genome shotgun sequence.
ACCESSION   DACEXQ010000124 DACEXQ010000000
VERSION     DACEXQ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.124"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..323)
                     /locus_tag="IXH04_001848"
     CDS             complement(<1..323)
                     /locus_tag="IXH04_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1852445.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFF"
     gene            complement(433..974)
                     /locus_tag="IXH04_001849"
                     /pseudo
     CDS             complement(433..974)
                     /locus_tag="IXH04_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
     gene            complement(992..2416)
                     /locus_tag="IXH04_001850"
     CDS             complement(992..2416)
                     /locus_tag="IXH04_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1852446.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLS
                     LNSKLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVK
                     NRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDIN
                     FDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQF
                     FTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSK
                     TNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGG
                     IKKDIKLRVEDSTLIEQIFNDITK"
     gene            complement(2418..3086)
                     /locus_tag="IXH04_001851"
     CDS             complement(2418..3086)
                     /locus_tag="IXH04_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAQ1852447.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(3217..3801)
                     /locus_tag="IXH04_001852"
     CDS             complement(3217..3801)
                     /locus_tag="IXH04_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1852448.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(4132..4365)
                     /locus_tag="IXH04_001853"
     CDS             complement(4132..4365)
                     /locus_tag="IXH04_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAQ1852449.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(4380..5330)
                     /locus_tag="IXH04_001854"
     CDS             complement(4380..5330)
                     /locus_tag="IXH04_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAQ1852450.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(5327..6169)
                     /locus_tag="IXH04_001855"
     CDS             complement(5327..6169)
                     /locus_tag="IXH04_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ1852451.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            6491..7678
                     /locus_tag="IXH04_001856"
     CDS             6491..7678
                     /locus_tag="IXH04_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ1852452.1"
                     /translation="MYFLGIDIGKRTHVASIMNEEGKVLLKGFSFPNTTEGAESLIER
                     MVDYSGAPSDFVIGMEATGHYWLSIFSYLHESDYLIHVVNPLQTDGWRKGTEIRKRKN
                     DIIDSVLIADLMRYGSFVETVLSDENVFSLKQLSRYRTYLVGTASDFKRKIIAVLDQV
                     FPEYATIFTKQGVFGKASKELLLAFSAPIDLENISSETLAQYLAELSRNRIKAEKADQ
                     LKQAASNSFGVKFAQEAFTFQLRSMIEQLKFIESQIKETEREIKHIMDTLNSVILTIP
                     GIGPINGATILGEIGDIQKFSNPKKLVAYAGIDASVTQSGQYEATHNVMSKRGSPYLR
                     KALFSAALVASQCDPVFSAFYQKKVSEGKHHLTALGAVSRKLCYVIHAILTKNEPYII
                     VSD"
BASE COUNT         2603 a         1428 c         1097 g         2681 t
ORIGIN      
        1 tcaaaaaaac gaaaacttgc ttctgttaaa ccgctaattc ccttttttct caaattagtc
       61 tctaagcgaa acccaaggat atgagaaaat aagccagaaa aaatataaaa taaagctcct
      121 aaagttaacc aagcaacagt ttgaaccgca taactcttag ctaaatattc atctcctaca
      181 ataattagag actgaagaaa tttgtaaata ctgtaatatc catagactgt taatacgaca
      241 gaaaaatcag aaaataaagt ggctaaccat ccgtagtatt tcattttagg gacataacta
      301 aataattttt tgtatacttt cattttttcc tcccccgcat tcaatttctt ctcttattcg
      361 tttacaactg ttaatgcttt ccgtaatagc gtaaattaga gatgtacaaa cagatagaag
      421 gtaaactacg ttctattttt tgtatttttt taagactttt cttccaatca atacaccaat
      481 catagataaa cttgctacaa ctaacactga aataattccc catataccca ccgcgtagtg
      541 gatcattgca tccattgttg cagcagattg tcctctatct atccagtctt gacgaaaacg
      601 atctgtaaag aaccatatgg gaatccactg tccggcagca ctgcctagtt gcattagaac
      661 ccaagacaca acttgaggaa ttgctcgatc atatccaaat acagatagaa taatatcagc
      721 taagatcgcc atactaatcc gcccattaac gtaaaaaata acaataaaac aattgtgtaa
      781 atagtaaaaa ttccttttct agggtagtta tagctaatat aaacaaatat gattccagat
      841 aataatccaa aaatccctgg ggatatgctg tgaccaaacg ccccaccaat actactaacc
      901 attattgaaa aaaagtaaat tactagggaa ataactgtag ctaaaccaat aaaaatataa
      961 tcttttgttt tcatatatat tctcctcaat tctatttcgt tatgtcatta aaaatctgtt
     1021 ctatcaaagt tgaatcttct actctaagtt ttatatcttt ttttattcca ccatttttta
     1081 aataaataat tctattgcat gttttataaa gaaattccgg atcatgagtg gctataacta
     1141 ctgcaccctt ttcttttaag tctttaataa ggtcagcaat cctttccata ttgatataat
     1201 ccaacccact tgtaggttca tcaaatagaa ataaattagt ttttgacaat gaagctatag
     1261 aaaccaataa tctttgtttt tctccaccgg ataaatcaaa cggtaattga tctttcattt
     1321 tataaagttc catttttttt aaaatttttt cttgctgatc tttactaact atttcattcc
     1381 ccagggctaa ctcgctcctc actgattctg tgaaaaattg gtaatccatt tcttgcatta
     1441 ctaaaaacgg cgatgacaaa ttttttttat tttcgattat ctttcctttg gtagggcgaa
     1501 taatcttcat gagtattctt agtaaggttg ttttccccac accgtttttc cctaccaaac
     1561 caataacatc tccaactttg acatcaaaat ttatgtcttt tagaatattt tcataagtaa
     1621 tttcttcaac ttttaataca tctgcactta ccttttcgat atagggaact tcaatcgaac
     1681 tattaaaaat gtcaaatttc cttaatgcgt tattgttttc agtttttaac aactgaatag
     1741 ttgtagtttt tacagttcta tttttaacaa atagaacttg atcaattaat tctttcaaat
     1801 agtaaaaacg atgttcagct ataacaatag taaatccttt attttttaaa tcacaaagta
     1861 agtcagcaag tttcttagtc gctttgaagt ccaaatttga agacggttca tccaatataa
     1921 ttaatttaga gtttagcgat aaactacatc caatagaaat tttctgccgt tcaccacttg
     1981 atagcgaaaa aatgttttta tctaatatat ttgaaagatc taataattca gctaattcat
     2041 tgattctttt atccatatgc tcttttgtaa atccaaagtt ttccatcgaa aatactaact
     2101 cactcgttgt atttgttgta aagaattgtc ctctaggatc ttgacttacg tagccaatat
     2161 tttttgatat actttcttgt gttaaggatt gtagcgagtt attttcaaat aaaatttccc
     2221 caaccaattt tccgtcataa agtcttggaa taagtccatt cattaacttt aatagtgtgg
     2281 atttcccact cccagaagaa ccagtaatca atgtaagact gccttttttt attgaaaaat
     2341 taaggtcaac aagcgcttgt tccttaacat ccttatatga aaaagaaaca ttgttaaatt
     2401 caaacaattc tttcacctca aatcaaaata cttaagaaaa aaatagtaac ggaaaacagg
     2461 atgatatagt ctaataagct catgtttgtt ttatagtaag aacttctttt cacaggagat
     2521 tctatcccct ttgttattgc attagcggtc atttcttcag caatcatagt tgcacgtata
     2581 agaacgggaa ctaacagaaa ttccaaatat tgcagaggct gaaaaaaaga caagcgaatg
     2641 tttctcattt ttaaagattc tttaatcatt cttatttcag aacgataagt cggaaagaat
     2701 ctgatcacta ctgtaatact taacattatt tttttcggta aatgtatttt ttctaaagag
     2761 gataataatt cattgctagt atagacacta accaacaacc atgagatcat tacgaccggc
     2821 aacattcgaa atacaatata taaggatgtc ccaaaaaata tagtaaaaaa ctctatcttg
     2881 atagcatcaa taattttcat tactaaaaac agaactgaat aaatcaagat gaaataaata
     2941 catgtcttta ttttacgaaa catcaatact aaaactgtag caataatgaa acaccccact
     3001 tgaaatatat ttccctttcc taaaaaaata atgaccggaa aaaaaatatt taaagtaatc
     3061 aaggttaacg gattgatttt tgacactctt tcacctccaa aaaataagat cgcactgtta
     3121 tcttaagctg acaaaaatgc accgttattc agataacagt gcgatcttat caaaaagcat
     3181 aactgagaac aattctcaat gcaagctttt ttttaattaa ctcagtaatt caatccaccc
     3241 aggaacagaa aattgataca atcttctggt taatagcata gtcttttctt ttgaatagct
     3301 atgcttcaat atatcataga atgtagcgta gaatgagtaa atgtagaagt ggatttcttc
     3361 ttcattaaag actaaatttt gattgatcat tccgatagtc tttaacttat ctagacaaca
     3421 gactgtcttt tttgtcacaa atgatgtaat tttttcaaca atatcttgat atttggttcc
     3481 agctgattgg aataaaacca aatcgaatat acgtttattc tcaaaaatat aattaataaa
     3541 tggatctaat tcattgaagt tctctctcca acttttctct atatctatat tttctaagtt
     3601 taagaggtat ttggtaaact cttcattata catattaata agcgcattaa tatgcggctg
     3661 aataatttct tcaaataacg cttccttaga agtaaaatgt ttataaaaag cacccgtagt
     3721 tacgcctgcg gtcaagcaca gttgccttat cttagtttgc tgaaatcctt ctttttcaaa
     3781 atggcttagt ccactttcta ttaatttttg gtgagttgta atataatctc tggtttctat
     3841 gggtacttcc tcctaataag atgaattaac tatattatct tacacaggac ttgaagtttc
     3901 aatcgttaac gtctattact gaactcattc cttagagcta aactaatgag aaagagcaaa
     3961 ataactgata caaaaagcaa tcctacaaga attaatagac tcatctaaat cattcacctt
     4021 ttctaaaata atccgagaaa gatttaaaaa aattatatcc agctattagg gtaagtccta
     4081 tcataatcac tccaatagcc attttcttat cctccctaaa aataaagtct tttacttaaa
     4141 accttttttg taatcgtcaa taaatttttt ccctttatca gttaattgaa atcctatatc
     4201 taaatagtcc tcatatctaa gtttagccag catctcactt acagatgctg gactaacact
     4261 tagaactttt gctaaatcat tattttttaa tttctttttt ctttttgata attcctgaat
     4321 tgctagtaaa taaattagta tagtagtcgt tttaatttgt accataccta ccctccaaat
     4381 tatttcgaaa ggccttcagc aattttatcc aggttccatt tcatcatcgc atagtaacta
     4441 tcaccatctt gtcctttttc tgcaattgaa tcagtaaaaa tcgttgaata gataggaata
     4501 ttggtatctt ttgatactgt tttcattggt ctatcgtcca cactgctttc tacgaataag
     4561 gagggaactt ttgttgtgcg taatttttca actaagtgtt ttatttgatc tggtgttcct
     4621 tcttcttcag tgttgatttc ccaaatgtaa gcagaaggca cattatacgc tttcgagaaa
     4681 tatttaaagc atccttcact tgtaacaatc atttttttat cattcggaat tgaagcaaat
     4741 ttagatttag cttctttgtc tagagaatcc aatttttcaa tatacttatc tagattttct
     4801 ttatagaatt ttttattatc aggatctttt tccgctaact gtttttcaat atttttagcg
     4861 taaataatac cgttttctaa atttaaccaa gcatggggat cttccttccc tttctcactc
     4921 tgaccctcta agtaaataac atctatgcca tcacttgctg caaaatagtc tttgttttcc
     4981 tctttgttcg cattttttac taattttgta aaccaagcat ttcctccagt ttccaagtta
     5041 acaccgttat aaaaaatcaa atctgctttt gaagtttttt gaacatcttc aggcaaaggt
     5101 tcatattcgt ggggatcttt cccaataggt acgatactgt gtaaatcaat tttatctttt
     5161 gctatatttt cagtaatatc tgctaaaatc gaattagtaa ctactacctt taatttttcg
     5221 ttcgaagcca ctgtatcttt gtctttacta tttgttgaac atgccccgaa tataaaaaga
     5281 gcaatcaaaa tccccagact tcccagtact gctttccatt ttctcatcat aactttctct
     5341 ccttctttct taaaaatttt tgttttggtg agattagaaa actaattaca aataagaatg
     5401 ccgctgttaa aacaatactt gatcctgctg ctatgttaaa actataacca atgaacaaac
     5461 ctagcaatga tgagcacgct ccgactgtcg ccgaaataaa tatcatagtt tttaagctgt
     5521 ttgcatataa atatgctgta gcagctggtg taattaacat tgcaacaatt aatatagttc
     5581 ccacactttg catagctgtt actgctacta aagttaatag aatcattaat agataatgat
     5641 aaaaactgac attcatcccc attgcttttg ccattaatgg atcaaaagaa gtgattaata
     5701 attctttaaa aaatagaaca actgtaacca acactaaaac agaaacgcca attgtaatcc
     5761 acttatcaat atcttgtaca gctaaaatat ttccaaataa gatatgaaat aaatccgttg
     5821 agctatttgc aactcctata aggataactc ctaatgctaa aaaagaactg aaagtaatac
     5881 caattgctgt atctcctttt attacactat tgctttttat aaaagtaata atgatagaag
     5941 ctaatagacc aaatacaatc gcaccaataa aaaaattaat acctaaaata taagaaattg
     6001 ctactcctgg caaaacagca tgagaaatag catcacccat aagagacatt cctcttaaaa
     6061 taataaaaca acctactgtt ccagaaacaa tccctataac tacagatgta attagagcat
     6121 tctgtaaaaa gtgaaagtca tacagaccat taataaaatt ttgaagcata ttattccccc
     6181 ttgtatatta gagttagggt tttaaccttc cggttttgat tggataaccc tatgaaccat
     6241 cgttatttta atttggtatt aactcatttc ttttgagata atatatttat tggattgaga
     6301 ggctaagccg ttcccccctt gggtcattga acccgcactc gagagactgg ctcctctctc
     6361 tgataagtgt attgcgtatg agttagatgt tgaaagtcct tactttactc cgtatctcgt
     6421 acaaggtagt gacgcttttc agttgagtgg atttcaatcc aaaattcttt ttttgaaagg
     6481 tcagatatac atgtactttt taggcattga tatcggtaaa cgcactcacg ttgcttctat
     6541 catgaacgag gaaggaaagg tattactaaa aggtttttca tttcctaaca ccactgaagg
     6601 agcagagtca ttaattgagc gaatggtcga ctattccgga gcacccagtg actttgttat
     6661 agggatggaa gccacggggc actattggct gtccatcttt tcataccttc acgaatcaga
     6721 ttatttgatt cacgttgtga atccccttca aacagacgga tggcgtaaag gtacagaaat
     6781 acggaaacga aaaaacgata ttattgattc tgttttgatt gctgacttaa tgcgctatgg
     6841 ctcatttgtc gaaacggtct tatccgatga aaatgttttt tctttgaaac aactttctcg
     6901 ttaccgaact tatttagtag gtacagcgag cgacttcaaa agaaaaatta tagccgtatt
     6961 ggatcaagtc tttccagaat acgcgacgat ttttaccaaa cagggcgttt tcggaaaagc
     7021 ttctaaagag ttattgctgg cgtttagtgc cccgatcgac ttagaaaaca tttcctcaga
     7081 aactttggcc caatacttag ctgagttaag caggaaccga atcaaggcag aaaaagcgga
     7141 ccaattgaag caagcagctt ctaactcctt tggagtaaaa tttgctcaag aagcgttcac
     7201 cttccaacta cgttcaatga ttgaacaatt aaaattcatt gaaagccaaa taaaggaaac
     7261 ggaaagagaa atcaagcaca taatggacac tttaaattct gttattctca caattcctgg
     7321 aattggtcct ataaacggtg cgactattct tggtgagatc ggagatattc aaaaattttc
     7381 taacccgaaa aaactcgttg cttacgctgg gatagacgct tccgtgacac aatcagggca
     7441 atatgaggca acgcataatg ttatgagtaa acgagggtct ccatatttgc gaaaagcctt
     7501 gttttcggcg gctttagtag cttctcaatg tgatcctgtt tttagtgcgt tttaccagaa
     7561 aaaagtttcc gaaggaaaac atcatctaac tgccttaggt gcggtttctc gtaagctttg
     7621 ttacgtgatt catgctattt tgactaaaaa cgagccttat ataattgttt ctgattagct
     7681 attgaacatt caaaaaaact aatggtaaga agcttatttg ttgtaccctt ttttcggaat
     7741 tatctgttat aaaatatttt ttgatttggc taatttttac ttgactctat atagttagtc
     7801 tctccaata
//
LOCUS       DACEXQ010000125         7797 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.125, whole genome shotgun sequence.
ACCESSION   DACEXQ010000125 DACEXQ010000000
VERSION     DACEXQ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7797
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.125"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..952
                     /locus_tag="IXH04_001857"
     CDS             <1..952
                     /locus_tag="IXH04_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAQ1852453.1"
                     /translation="NEKYKKMKLSSKLVYMLLKDRNRLSLKNNLYDENQKIFLIFEIK
                     NLVEISGMSKNTILSALDELQKVKLLVKRKNGFNKSKMQMFPNFYYLLKPVLCKDDVY
                     KINKITHQENRQKNLIVQNLNYGTNQYSVDNSKFELCDTSMKTRDKNNFADSSKNELE
                     LYKSYKDSKDIKESHNDDLLESLSTKNNKLDEELIETFIEENLLKQIWGESIVNCLKL
                     ASNSDYRQFDNWYKKFKYAIRSAEKEQKIQLKIIYEICNNKYFVDHVREQLSMTLRDL
                     IRRAKTDHRIKQKDDYIFASLKNKALELIELQISEGIDKQ"
     gene            1028..1246
                     /locus_tag="IXH04_001858"
     CDS             1028..1246
                     /locus_tag="IXH04_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852454.1"
                     /translation="MIKNRLIDQVWCLDQNTDSLELIETICFNTIVLDLRAENDNCVT
                     HVIINQKMAQKLSESLRKWAKGGNDENN"
     gene            1233..3911
                     /locus_tag="IXH04_001859"
     CDS             1233..3911
                     /locus_tag="IXH04_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852455.1"
                     /translation="MKIIEKKIANKKINWLVVNFLVLIAMIIFTSNIAHASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSAVNTIVIGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSIALI
                     LLMPTMISSGIQFAKTFYKDSTTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNNLTRKNFQQTDLTQVLTDKVIDKLEKENPIADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNSGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSNGFNRAKGSGE
                     GEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERATKPFKSMRDI
                     ANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSVPTRLSDRKDGAKVEN
                     ADRIMSSSERMREAMKNNAESNNNPASAIQQKVQQDIEERKNAMHGQAKSAEELINQK
                     RHEAKYTPEAMNREEIMLRKRVEGRTGANIDGKEALTKERIQEAKNSNTGLEKLVKEN
                     PQNSTSSHGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNAQGTKTIDVRENLQSS
                     STSQPKTVDVRENVQNSTSSQGFKSVDVRENVQGSSASQPKTVDVRENVQSSTSSQGF
                     KTVDVRENIQKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKSR
                     RRFTYEDNELFRDTLNDPNPLFDNLLRNKRGGLSCN"
     gene            3902..4099
                     /locus_tag="IXH04_001860"
     CDS             3902..4099
                     /locus_tag="IXH04_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852456.1"
                     /translation="MQLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKNEIESNFKCCSLEKNVRG"
     gene            4103..4714
                     /gene="mobC"
                     /locus_tag="IXH04_001861"
     CDS             4103..4714
                     /gene="mobC"
                     /locus_tag="IXH04_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HAQ1852457.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVVLTKQLNQIGNNLNQAVKALNIIAKYFREESEDKKEK
                     SLHFSFDASRYNIFKDELANSIAKKLMISYPDDIFLESNRSAPSFFKLSEVEKEYFEY
                     WIEKQTRPGTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            4699..6090
                     /locus_tag="IXH04_001862"
     CDS             4699..6090
                     /locus_tag="IXH04_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAQ1852458.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDDSEE
                     ALQEMNLVRTKMNKSKDIAQAFHVIHSFDKSTSKELSIDKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNINMETCKRLRIDPKDLELMRKINDQVLNEHGLESVNNEY
                     YENLEKRTNLYSNDPSKMKYGKINYQEFVQNAIEATLEKCEVNSFEDFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQGVNFYERRGELITLGRDKQSRKEKSQYVERTFSARKLGK
                     RYSFESVSEQLEKNLEESHIKNFFNMEREQVMSLISGVNYKTGELNVPFVELDSIAQK
                     QFSDREGDEKFIKYDDFLREVDKHFVNTMSLEDDQEYTIKLHTDVNPQTLRSAKSKTI
                     SIKKTDQKELFLPAKGFENCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQKRQQNFEMEF"
     gene            6306..6620
                     /locus_tag="IXH04_001863"
     CDS             6306..6620
                     /locus_tag="IXH04_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852459.1"
                     /translation="MNYPYFKVSASEETKEIFNNFYNQNKGIFGSKANMFRVMVSNLP
                     VLASPSNNKFNDPESIKFEQKISELESMISNEVIEKLDDIDQKLSYSLKNKYKTEEKK
                     DV"
     gene            6613..7107
                     /locus_tag="IXH04_001864"
     CDS             6613..7107
                     /locus_tag="IXH04_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852460.1"
                     /translation="MFKVYFPFQWLVKGILLVLLGLILFGSYLTIKARHFPEEKPIKI
                     VTKDLKIGETYQADGVELTFKNIEVTEKRNEFAQHDRVLQVTYTLINDTENPIRPQQW
                     SIENKDGEELYEYDMILDNPEYIESGKEETVNYYYELPIERQDYQIVVRRENGYIVGL
                     PFTL"
     gene            7175..7489
                     /locus_tag="IXH04_001865"
     CDS             7175..7489
                     /locus_tag="IXH04_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852461.1"
                     /translation="MAEENKVVPFETLVINKEEFEQLFLDFFIQYDMIYDFSSSLDLF
                     ELEAEIIENSREKNGKIIISVEKFNELFSQTFEIDPDYYQPQEVFREFRNKIIGFSQT
                     TK"
     gene            7588..>7797
                     /locus_tag="IXH04_001866"
     CDS             7588..>7797
                     /locus_tag="IXH04_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ1852462.1"
                     /translation="MDTITPTNFKENIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSDL"
BASE COUNT         3020 a         1043 c         1481 g         2253 t
ORIGIN      
        1 aaatgaaaaa tataagaaaa tgaaactaag ttctaagtta gtttatatgt tactaaaaga
       61 tagaaataga ctttcattga aaaataactt gtatgatgag aatcaaaaaa tttttttgat
      121 atttgaaatc aaaaatctcg tagaaatttc gggaatgtca aaaaatacaa tcttatcagc
      181 actagatgaa cttcaaaaag ttaaacttct tgtgaaaaga aaaaatggct tcaataaaag
      241 taaaatgcaa atgttcccaa atttttatta tttgttaaag cctgttctgt gcaaagatga
      301 tgtttataaa attaataaga taacccatca agaaaataga caaaaaaact tgatagttca
      361 aaatttgaac tatggaacaa atcaatattc tgttgataat tcaaaatttg aactatgcga
      421 cacatcaatg aaaacaagag acaaaaacaa ttttgctgat agttcaaaaa atgaactaga
      481 attatataag tcttataaag atagtaaaga tattaaagaa tcacataatg atgatcttct
      541 tgaatcactt tcaactaaaa ataataaatt agatgaagaa ttgatcgaaa catttattga
      601 agaaaatctt cttaaacaaa tatggggaga aagtattgtg aattgcttaa aactggctag
      661 caatagtgat tatcgtcaat tcgacaactg gtacaaaaaa ttcaaatatg cgattagatc
      721 tgctgaaaaa gaacaaaaaa ttcaactgaa aattatctat gaaatttgta ataacaagta
      781 ttttgtagat catgttagag aacagctatc aatgacttta cgtgacttga tacgaagagc
      841 aaaaacagat catcgtatca aacaaaaaga cgattatatc tttgcttctc taaaaaataa
      901 agcgttagaa ctaattgaat tgcaaatcag cgaaggaata gataagcaat aagaatagaa
      961 ttgagtcaaa tgaaatggag gggtatctcc acttcatttg agaactttga atataggagg
     1021 ggttattttg attaaaaatc gtttaataga tcaagtttgg tgtctagatc aaaatacaga
     1081 tagtctcgaa ttaatcgaga ctatttgctt caacactata gttctcgatt taagagctga
     1141 aaatgataat tgtgtaactc atgtcataat taatcaaaaa atggctcaga agctctctga
     1201 gagcctcaga aaatgggcta agggaggtaa cgatgaaaat aattgaaaaa aagattgcca
     1261 ataaaaaaat aaattggttg gttgttaatt ttcttgttct gatagctatg attattttta
     1321 caagtaatat tgctcatgcg agtattttca attggtttga tgacacaaaa agtcaaacag
     1381 agtttttgaa taattctctt tatagacctt acctaaaaaa gtacaatgga gatttcctta
     1441 gtcagttcgg gatttggctc ggatgggctg tggtaaaagg catgtttact gtgacagatt
     1501 cgattcaaaa tatgattcca gatgtgctgg atttgttcaa tttcattgaa agtacagggc
     1561 taaataatgt ttatcaatca gccgtaaata caattgtcat tggattaatg atcctttcat
     1621 tgatgtttgt tggttacaaa atgattactg gaaaaggaac agtggattta aaatcggttg
     1681 gaatgaacgt agtgatgtct atagcattaa tacttttaat gccaaccatg atttctagtg
     1741 gtattcaatt tgctaaaaca ttttataagg actctactac gattacaaat tcagatgacg
     1801 gagtcgcttg gtcattgata aaacaaggag taacagattt agcatatatt aataaaacag
     1861 atcagtactc aagcattgat aaaacggagg atcgaaataa tttaacacga aaaaattttc
     1921 aacagacgga tctcacacaa gtgttaacgg ataaagtgat tgataaattg gaaaaagaaa
     1981 atccaattgc ggacaatctt cgttatgaat tagttgaaaa ttctaataat gaatttgttg
     2041 ctacaaagtt ttctgacaac ttcttatcaa cattttctga tagcttgaaa tcaggctatt
     2101 accgatacca agcaaatcta tgggggattt caattgggtt gacagcgtta gcaatcgcct
     2161 atgtgttcag cgcgtttgtg atcattacag caatactaga acttgcattt aaacgtgtgt
     2221 taggtgttct tgtttttgca acagatattg agacaggaca gcgttcaaaa gttgttctat
     2281 cagatattct acaatgttac ttaactgttg ggtttcaagg atttgggtta tcaatgtttg
     2341 ctatgtttat caattttctg aactcaggac aaggtatctc aacaaatatt tttataaaaa
     2401 caattgctta tatctgtgcc gtttttgttt taatcaaagg cagtggaaca gtcatgagat
     2461 actttggtgt tgatattgga ctcaaagaag gctatgggca acttgcttct gcttttggaa
     2521 tgggcgcaat gctgtttaga aaaggatcaa atggatttaa tcgtgctaaa ggaagcggag
     2581 aaggagaaga tcgaaaacca gaaaagaatt tcggcgaaac tctatcaaaa aaagctggca
     2641 aaactggaag agctttggga tatgcccatg aacgtggatt atccggttta gcttcagatg
     2701 gagctacaat ggctagtgaa agagcaacaa aaccatttaa gagtatgcgt gatatagcta
     2761 atgataccaa gaataagttt aaagaaggtt tagatgatgg tacagtgtcc gctattaata
     2821 aaaatagcaa acctatgtta gctaaaaata aagaagatga aactggaaaa tatgctgatt
     2881 ccgtgccaac acgtctttca gatagaaaag atggtgctaa agttgaaaat gccgatagaa
     2941 ttatgtctag ttctgaacgt atgcgtgaag caatgaaaaa taatgctgaa tcgaataata
     3001 atcctgcaag cgcaattcag caaaaagtcc aacaagatat tgaagaacgt aaaaatgcta
     3061 tgcacggtca agctaagtct gctgaagaat taattaatca gaagcgtcat gaagcgaaat
     3121 acacgcctga agcaatgaat cgcgaggaaa taatgttaag gaaacgtgta gaagggcgga
     3181 caggcgctaa tattgacgga aaagaagcgt tgaccaaaga aagaattcaa gaagctaaaa
     3241 attcaaatac aggattagaa aaattagtca aagaaaatcc acaaaattct acaagtagtc
     3301 atggtggccg atcagtagat gtccgagaaa atattcaagg ttcactgaat ggtcatgttg
     3361 gacggaatgt tgatgtaaga gagaatttac aaagttcttc aaatgctcaa ggtactaaga
     3421 caattgatgt tcgagaaaat ctgcaaagta gcagtacaag tcaaccaaaa acagtagatg
     3481 ttcgagaaaa tgtacaaaat tctacaagta gtcaagggtt taaatcagtg gatgtccgag
     3541 agaatgtgca aggtagcagt gcaagtcaac caaaaacagt agatgtccga gaaaatgtac
     3601 aaagttctac aagtagtcaa ggattcaaaa cagtagatgt tcgggagaac attcaaaaag
     3661 atagtggaga aatgaaagaa aaaacacaaa aaatcaatat cgttgaagac agaaagtcag
     3721 cgaaaaaatt tgattcaaat catgaaacag tgattattga tagtgaaatc cgtgaaaatg
     3781 ataagggatc aaaatcaaga cgaagattca cttatgaaga caatgagtta ttcagagata
     3841 cacttaatga tcctaatcca cttttcgata atttattaag aaataaaagg gggggattgt
     3901 catgcaatta attctaaaga ttagtacgca tgatttctta gaacttgacg aattatgtga
     3961 atcagtaaag aacgcaacaa atgaaaaaat cacggtcact aattatattg aactgatgat
     4021 tcaagaagaa ttaaagatga aaaatgaaat agagagcaat ttcaaatgtt gctctctaga
     4081 aaaaaatgtg aggggataat caatggatga taaacaaaaa atgtacgaaa aaaatcgcaa
     4141 acatagtgag gccttaaatt ttcgtgtcga taaaaagact tatcttgaac tgacattgat
     4201 tgcaaaacaa aggggaattt ctagagcaga cgtttgccgt gagcggttaa agggactcaa
     4261 atatgaaccg ccgttgttag ctgaagatga tagggtagtt ttaaccaaac agttaaatca
     4321 aattggtaac aatttgaatc aagcggtaaa ggcattaaat atcattgcta aatattttag
     4381 agaagaatct gaagataaaa aagaaaagag cctacatttt agttttgatg ctagccgtta
     4441 caacattttt aaagatgaac ttgcgaatag catagcaaaa aagttgatga ttagttatcc
     4501 agatgacatt tttttggagt ccaatcggag tgccccctct ttttttaaat tatcagaagt
     4561 agaaaaagaa tatttcgaat attggataga aaagcaaact agaccgggta cgaaagaaga
     4621 gtattcccaa aaagttgatc ggaatttaat tcactcaatt cagattattg caagtttaaa
     4681 agatgaggtg aaacaacttt gggagctact ataacatcga aaggacgcaa gaaaaattta
     4741 tcctcgctat tacaatatgc agctaatcct tcaaaaaaag aaaaatgtgt tgcagtctca
     4801 ggaataaatt gtagtgatga tagtgaggaa gcactgcaag aaatgaattt agtacgtacc
     4861 aagatgaata aatctaaaga tatagcacaa gcgtttcatg ttattcattc gtttgataag
     4921 agtacttcta aagagttaag tattgataaa atgcacgaaa tagctgtcga atttgcagag
     4981 aaagcttttc caaattcgca aatcattgtt gcaagtcaca atgataaaga tcattttcat
     5041 agtcatcttg tcattaataa tattaacatg gaaacatgta aaaggttgcg tattgaccct
     5101 aaagatttag aactaatgcg aaaaattaac gatcaagtcc tcaatgaaca tggtcttgaa
     5161 tcagtgaata atgagtacta tgaaaactta gaaaagagaa ccaatcttta ttcaaacgat
     5221 ccttctaaga tgaaatatgg aaaaattaat tatcaagagt ttgtgcaaaa tgcgatcgaa
     5281 gcaacgttgg aaaaatgcga agtgaattct tttgaagatt tttcggatca tttggcaaaa
     5341 gaatataaca ttgaagttta tcaattttca aaaacaaata acaaacttgg ctatgttctt
     5401 tacaaacaag gtgtaaattt ctatgagcgt agaggtgagt taattacttt aggaagagat
     5461 aaacaatcta gaaaagaaaa aagtcagtat gtcgaacgta cttttagtgc aagaaagtta
     5521 gggaaaagat attcatttga atctgtttct gaacaacttg aaaagaattt agaagaatca
     5581 catataaaaa atttttttaa tatggaaaga gagcaagtta tgtcgctaat tagtggagta
     5641 aattacaaaa ctggtgagtt aaatgtaccg tttgtggaat tagattccat cgcacaaaag
     5701 caatttagcg accgtgaagg cgatgaaaaa ttcattaaat atgatgactt cttgcgagag
     5761 gtagacaagc attttgtaaa tacaatgtct ttagaagatg atcaagaata cacgattaaa
     5821 cttcatacgg atgtgaatcc tcaaacttta cgtagtgcga aaagtaaaac aatttctatt
     5881 aaaaagactg atcagaaaga gttatttcta ccggcaaagg gttttgaaaa ttgttctcat
     5941 acaccttctg ctaaacagat tcactatcaa atcagtcgca taatgaatga cgatgtaaaa
     6001 atgtatcgtg ctaaaaagga ttatgaattc acacaacaaa tgattgaaaa tcagcaaaaa
     6061 aggcaacaaa attttgaaat ggaattctga atgggcttgt tagtggactt tccagttttt
     6121 tgcctatgaa gagttgcttg gaaagaagca gtttttagga catacgccag ctacccatct
     6181 acttgccagt caatccgccc cgctcacgct aggctacttt ctggcaagtc agatggcggg
     6241 taaatctctg agggagcaag ctccctacag actccctccc aaaaaaagaa aggtggaaga
     6301 aaaaaatgaa ctatccatat tttaaagtct cggcttcaga agaaactaaa gaaatattca
     6361 acaattttta caaccaaaac aaaggaattt ttggttcaaa ggcaaatatg tttcgtgtta
     6421 tggtttcaaa tttaccagtg ttagctagtc catcaaataa taaatttaat gatcctgaaa
     6481 gcattaaatt tgaacaaaaa atttcagaac tagaatcaat gataagtaat gaagtaattg
     6541 aaaagctgga tgacattgat caaaagttat cgtattctct aaaaaacaaa tacaaaacgg
     6601 aggagaaaaa agatgtttaa agtttacttc ccgtttcagt ggctcgttaa aggaattctt
     6661 ttagtgctac taggattgat tctttttgga agttatctaa caattaaagc cagacatttt
     6721 ccagaagaaa agccaataaa aattgtaaca aaagatttaa aaataggtga aacttatcag
     6781 gcagatgggg ttgaactaac atttaaaaat attgaagtga ccgaaaaaag aaatgaattt
     6841 gcacaacatg atcgtgtatt acaagtgacc tatacactta taaatgatac agaaaatccg
     6901 atacgtccac aacaatggtc gattgaaaat aaggatggtg aagaacttta tgagtatgat
     6961 atgatattgg ataatccaga gtatattgaa agtggcaaag aagaaacagt aaattactat
     7021 tatgaattac cgattgaaag gcaggactac caaatcgtag tacgtagaga gaatggttac
     7081 atagttgggt tgccatttac tttataagtt ttattttgtt agtagttttt aaggtaatat
     7141 cagttttgtg atttttgaaa atggaactgg gagaatggct gaggaaaata aagttgttcc
     7201 gtttgaaaca ttagtgataa ataaggaaga gtttgagcaa ctttttcttg atttttttat
     7261 tcaatatgac atgatttatg attttagttc gtcactagat ttatttgaat tagaagcaga
     7321 aataatagaa aacagtcgag agaagaatgg taaaatcatt atttcagtag aaaaattcaa
     7381 tgaattattc tcacaaacat ttgaaataga tccagactat tatcaaccac aagaagtttt
     7441 tcgagagttt agaaataaga ttattggttt tagtcaaact acaaaatgaa agcttaataa
     7501 agcaagtgtt gatagaatta ttcctcaacg cttgctttat tttatgggca cttcataata
     7561 gaggtataga gaagagaggt attcattttg gatactatta ctccaacaaa cttcaaagaa
     7621 aatataggta aagtgttgca agaagtagtt cattcaaatt ctgaaataga aattactagg
     7681 gattctaaaa atggattgaa cgatggcgtc attatgattt ctaagagaaa atgggagttg
     7741 ttacaagaag aactttattt acaacgaacg ggtacatggg cttatgtatc tgattta
//
LOCUS       DACEXQ010000126         7750 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.126, whole genome shotgun sequence.
ACCESSION   DACEXQ010000126 DACEXQ010000000
VERSION     DACEXQ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.126"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(264..1274)
                     /locus_tag="IXH04_001867"
     CDS             complement(264..1274)
                     /locus_tag="IXH04_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1852463.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(1286..2239)
                     /locus_tag="IXH04_001868"
     CDS             complement(1286..2239)
                     /locus_tag="IXH04_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1852464.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            complement(2229..3299)
                     /locus_tag="IXH04_001869"
     CDS             complement(2229..3299)
                     /locus_tag="IXH04_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1852465.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            complement(3305..4348)
                     /locus_tag="IXH04_001870"
     CDS             complement(3305..4348)
                     /locus_tag="IXH04_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1852466.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            complement(4349..5290)
                     /locus_tag="IXH04_001871"
     CDS             complement(4349..5290)
                     /locus_tag="IXH04_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1852467.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            5374..5721
                     /locus_tag="IXH04_001872"
     CDS             5374..5721
                     /locus_tag="IXH04_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAQ1852468.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            complement(5791..7452)
                     /locus_tag="IXH04_001873"
     CDS             complement(5791..7452)
                     /locus_tag="IXH04_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1852469.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
BASE COUNT         2182 a         1594 c         1396 g         2578 t
ORIGIN      
        1 acctaatcat tcaagaaaaa acgcaaaaac aactctttgt gagttacgtt ttttgtttca
       61 gaattttgat gtattcgtaa actttctgaa taaaaaatta acaatagcag aaaaagctcg
      121 tacgaacttt ttctgctatt gttttattgg tttgtcataa ttagtgagct tttcttcaaa
      181 agaaaaattc ctagaaacaa aagtcattaa gactttttat taacaaggct aaccctaacg
      241 ctcctatcag tttcattcat tttttatccc ttgataaccg aatgtctgcc cattttttct
      301 actacttggc tctttcttac ggaattgctt tcttctgctc cgtcggtatt gatcaaaaga
      361 aattttttat tataccattg ggacttataa cgcaattttg ttattaattt atgccagtcg
      421 tgaatagaaa aatcagcatg tgcctcttct tttggaaaag attctacaaa taagtagtca
      481 tattgtttga ccaatttccc agataattgt tcaagataat cttctcgctg gttcttttgt
      541 ttcagataaa gatccattac ttttctcttt tgtttctgat aattgcttgc ctgatctaac
      601 ctgatctttc tatggtgagc ggagcggact ttcacattca atttccgctg tgcatgttgc
      661 agcttttctt ctgtctggtg aaattttggt aaagttactt ctagaggtcg agatgtttgg
      721 atcaatgtct tcggatgata ggcgatcccg atccatttag ctaaagattc tttttttact
      781 ggcgaaacat cacaaagtag tgcgacataa aattcttcat tatatcgagc agaaatcgta
      841 gcggagcgaa tcgatccttc aactggccga tgctgattta tcttgattag ttctttttgt
      901 tttggtaact ttaagtagcc attttccaga tagatcgtac gggtttggtt gttggtcgta
      961 taagattgcc acatactttt tttcgtttta agctttggga agctggcacg tcccttgaaa
     1021 taattgcgaa aagcccgatc caaattaagc tgtgcatttg ctaaagcaag actgtctact
     1081 tcttttaaga atgggtattc tttttttaat gtagcgggtg tgagcgaaaa attggctact
     1141 cctgtttgtt gccaactttt ctgccgcttt ttcagcaaat ggttataggt aaatcgagca
     1201 catccaaaag tacgaatcaa ttgctgtttt tgttcttctg tcggatacat acggaattta
     1261 aaagctttta gcatctatgt ccctcctagt tagcaaccag aagagattcc ttttgtcgtt
     1321 ttagtttttt tgcgtaatat gggacttcct gctcagttgc atagacaaaa tgttctggtg
     1381 cgatttcacg aagttgacgt acttgtccgt catctggttc aggttgatat ttgattcttc
     1441 tgcgttttct ctcatggtct ggatctggta gaggaatagc agataacaaa ctttctgtat
     1501 aaggatgaac accgtgatta tagatttcat cacttgttcc catctctaat aacaagccgt
     1561 tatgcattac accgattcgg tcactaatat gtttaaccat cgacaaatca tgtgcaataa
     1621 atagataggt caattgatgt tctttttgga gatcttgcaa caaattgacc acttgggctt
     1681 ggatcgacac atctagtgca gaaattggtt catcacaaat gataaaacgc ggttttactg
     1741 ccaatgctcg tgcaatacca atacgctggc gttgcccacc tgaaaattca tgaggataac
     1801 gagtaccgtg gcttggattc aatccaacag ttctcaatag atcatcgact ttttttgcac
     1861 gctcttcaga tgatttagct agtccattaa catcaattcc ttcggcaatg atatctctga
     1921 ctttcatacg aggattcaac gaagcgtatg gatcttgaaa gatcatctga acatcatgcc
     1981 ggaatttcgt caaacctttt ttatctttga tcttcgttat atcttgaccg tcgaataata
     2041 tctcaccatc agtaggttca ttcaagcgaa tgatcgtacg gccagtagtg gattttccgc
     2101 ttccagattc tccaactaaa ccgaatgttt ctccttcata aatgtgaaag ctgatgtcat
     2161 ttacagcatg tacttcattc ttttttccta cattgaaata ctgtttcaat cctttaactt
     2221 caagtagttt atttggcatc agcaattcct ccttgaccgt aagtatgttt ttgtttctct
     2281 ttaaagatag cccagcgacg ttggatttct tctggcggtg tcactttggg tgcctgcgga
     2341 gcaagtagcc aagttgctgc cttgtgtgtt tttgatagtt caaaaaatgg tggttcttgt
     2401 tctgcatcga ttttcatcgc gaattcattt cttggataga atgcgtctcc ttttggcggg
     2461 ttcaataaat ccggtggaga accaggaata gcatacaatc gatcgttcgc actatctaat
     2521 gtcggcattg aaccaagcag tccccaagta tagggatgtt gcgggttgta gaaaatttct
     2581 tccgatgttc caacttcaac gattcttcct ccatacatga cagcgacacg atcagcgaca
     2641 ttcgctacta caccaaggtc atgagtgata aagataattg atgtatcgat ttttgtctga
     2701 atttctttca gcagctccaa aatctgtgct tgaatagtca catcaagcgc tgttgtaggt
     2761 tcatcagcaa tcaggatttc cggataacat accagtgcaa tcgcgatcac gatccgttga
     2821 cgctgacctc ctgagaactg atgcggataa ctgttgatac gtttctcagc ttcgggtatt
     2881 ccaactaatt taagcaattc taatgcagct ttttgtccct ctttttttga aactttatta
     2941 tgctttctaa gggactcagc gacttgcttc ccaatgggca tcgtcggatc taaagaggtc
     3001 attggatctt ggaaaatcat cgctatttct ttaccacgga ttttttgcat ctctctttct
     3061 gattttccaa cgatatcgtt acctttaaag aggatttgac cagcatctat attggcgttg
     3121 cttgacagca agcgcatgat gcttctggtc gtcactgatt ttccacttcc agattcccca
     3181 acgatcgcca atgtttctcc tttattcaaa tgaaaattga caccgcgaat cgctcgtacc
     3241 tttccagcat acgtatcaaa agagatttct aagtctttca cttctaaaat tttcgtcatc
     3301 gtcttcactc tttcatttta ggatcaaagg catcacgcaa tccatcagca agtaagttaa
     3361 agccaatcat gataaccgat aaagttactg ccggaatcca taatagataa ggcagataca
     3421 ggaatgtctt gtacccttcg ctcaacatcg ttcctaatga agctgacgga ggcgtaagac
     3481 ctaaaccgat aaagcttaaa aaggcttcga agaaaatagc agatggaata ctaaacatca
     3541 tttgaataat aatgacacta gagatattcg gcaaaatatg tttgaatgca attttcattc
     3601 ttgattcacc taatgtcaat ccagctaaaa catattcttg atcttttaat ttcattgttt
     3661 gtgcacgaac gatacgtgcc attggtatcc agtttgtgat ggccatagca gccacgattg
     3721 aaaaaattcc tggttcaaaa acgaccaaca tcaaaatcat gacaacgagg tttggaattc
     3781 ctgataagat ttccaaaaaa cgttgcatca cgttatcgac acgtccgcct aacaaaccag
     3841 aaaccaaccc gtaagctaca ccaatcgtta catctagtaa tgcagcaata aatgcgatca
     3901 gcaacgagat tctcattccc ataaataagc ggcttaatac atcacgtccc aaaccgtctg
     3961 ttcctaaaaa gaaatttacg tttgaaggga cgtttgcttg agcatattta tcaacaagtt
     4021 caccaccaac cattgctttt ccattcaatc catcgatatt gattcccgga atacgaggag
     4081 gcaaattgat ataagccacg ttttgggcag ttggatcatg aggagaaaca aagattgtga
     4141 tcacagagat caaaatgatg accaatagta aaaccattga aataacggca gctttgtttt
     4201 tctttaaacg gcgccatgaa tcttggataa aattcaaaga aggtgcagta atctgttcac
     4261 gttcttcttc ggtatttttt tgtaatggaa gaaattcatc cgcagggata tctgcaactg
     4321 tttcataacg ctttggtatc atttccattt atcctcggct cccttctgaa acacgaattc
     4381 ttggatcaac tagaccgtac aatagatcca cgatcaaaat aacaaaaatc agcataaatg
     4441 agtaaaggat cgttacagcc atgatcgtag gataatcatt tgttgtgatt gacttaacga
     4501 attgttcccc gataccagga atagcaaaga tattttctac caccagtgat ccggtcatca
     4561 atgctacagc taacggtcct aataaagtca tcaatggaat caaactattt cgaagaccat
     4621 gtttaaaggc gatttcccaa cggctcaatc cttttgcttt tgctaattcc acataatcac
     4681 tatgcagtac ttccaccatt tccgtacgga taaaccgagc agagtcagca agcggcgaca
     4741 tggccaacgc aattgttggt aagattgtgt acacaaagcc atcccatttt gcgattggca
     4801 aaacttgtaa tttgattgca aagatatatt gtaataaaac agcaaataca aagtttggaa
     4861 tggaacgacc taatatcgct acgagtgtag aagatgtatc tgcccatgta ttttgcttca
     4921 tggcagagat cgttcctaaa atcgtcccta ggactgttcc aaagataatt gcctgtaacc
     4981 ctaactgcaa ggatggtccg attcttccag ctaacaaatg tgcgactggc tggtttttaa
     5041 attggaagga aattccaaaa tctccttgaa gcaaattaga aagataaatc ccatattgta
     5101 cgataactgg tttatccaaa ccgacttgtt tatttagcat ctcgattgtt tcaggactca
     5161 aacgttcctg attcgtatat ggcgtgcccg gcagcaattg catcaagaaa aaggtgattg
     5221 ttgcaattaa ccaaagcgtg atgatcatga aaaagacacg ttttaataca tatttgatat
     5281 aactattcac taatgtttcc ccctagcttt tgacttctga cagatgtttc gctacattat
     5341 tagataatga accaaaaata aaggagcaat caaatgaaaa aaaaatctat cccctacgca
     5401 gtagcttttc tactgattct ggttatttta ataaaaaatc tcatcaacca ttcctttaca
     5461 ctcattcaat tatctaatga tctattctta tggtcattgc cgtttctgat tatcggcggt
     5521 tttttatggg tcttttcttc gggtttcttc gatcattttc agcgatcggt ccatcttgca
     5581 cgaacaagga accgaaagaa aaaaccagaa ttttcctcgc tctcgtctgc cagctacggt
     5641 atgtacagct tttggctgat cattgccgga atactaattg ctctttctgc tattttcatg
     5701 cttttttctt tgttaggata agtgaatatg agaacgaggt tgcgacattt gccgcagcct
     5761 cgttttttct catgctcacg gtattaacga ttattcttca acgtatgtcc atttgtaatc
     5821 ccatgaagcg cctgccgcat ggtgaacgat acctttgatt ttttcattca ctaggtgacc
     5881 ttctgctttt tggtacaccg gaacgacacc catatcatca gagatgatct tgattgcttt
     5941 ttggaaatca tcccaacggg cttgttcatc gccagcgtct gttgatgaag ctgctttaac
     6001 tgcttcgtca tattctgcat tgctccagcg accacggttg tatgaattac ctgtgacaaa
     6061 caaatcagta aagctgcttg ggtctgcata gtcagcagcc catccgccta gtactgcgtc
     6121 aaaatcaccc gaagttgagc ggtcaagacg tacagagaat ggaacaggtg ttggtttgac
     6181 tgttacgcca tccaagtttt cttggattga gttttgaaca tattcgatta cttttttcga
     6241 tgaatcatca tcagaagcca tcaaatcaaa ttctaaagaa tcaacgccca attctttttt
     6301 cgctttttcc caatattctt ttgctttatc tttgtcataa gaaactaatt ttccagcttc
     6361 ttttgcaaag tcttcgttcg ttgttggact ttttaccata tctgatggga tcaatcctgt
     6421 agagacaact gatccgtcac ctagtacttg ttttactaaa gcttcacgat cgatagaata
     6481 agaaatagct tttcttaggt tcacattgtt gaatggtgaa ttcttttcac gttgattgaa
     6541 ttcgatatag cttgtccgcg cttctttaac agaagtataa gctggatcgt ttgcgttttg
     6601 ctgtgctaac tcaccagaca agatcacgtc atctgcttgg ccatctttga ataagttcaa
     6661 tgcagtagaa gattctttta ctacactgac tttgatttca gaaagtttta ctgtatcttt
     6721 gtcccagtaa tgatcatttt tcttataagt ccattctgta tctgttcctg gtccatcaaa
     6781 atcttctaat gtgaatggtc cattataaac agctttatca cttgcagatg cataggcatc
     6841 cccattttct tccactacgg attgtttttg tgggaaaaat gatgggaaag caagcagata
     6901 ttggaaatat ggtgtttgtt tcgtcaattt gatttctaat tcatagtcac caactgcttt
     6961 gatccctagt tctgatttat ctttctttcc agctgtaatg tcttctgcat tttcaactgg
     7021 cgcatacagg taagcatatt cagaagctgt agctgggtct actgttcgtt gccagccata
     7081 aacgtaatcc gctgctgtaa caggatcacc atttgaccat tttgcgtctt ctcgtaattt
     7141 gattttgtaa gttaatccgt cttcactgac ttctgccaac tcgctagctc ctgccggctg
     7201 tggtttgctg tcttcatcaa gacggtaaat tccttcataa acgttattca atgctgtaaa
     7261 actgattgta tctgttgcaa ctgataagtc agctgtaggc atttcttgag gcacgactaa
     7321 gttaaatacg cctcctccat ctgctgaacc gctttttgct gaactactgc ttgtggctga
     7381 actgccgcca taacatccag caagtacaag gccgcatacc gcaaccaaac caaatgtaaa
     7441 tttctttttc attttacgta cccctttatt ggaattttca gaataattaa tattttctga
     7501 agttgtttaa gagtttaatg aataattatt aaaaaatcaa cacttttttt tgtctttttc
     7561 ataaaataat gattttttcg tgaggtttca aggaattttt cagaaaaaat agctttgtac
     7621 aaggttttcc atggtcttcc aaaagccatt aaataatttt cgttatattg tctattattg
     7681 ccatacaaag ataaaaaaga ataaacaaaa agaaactgtg acaaaagtct tgtcacagtc
     7741 ccttatctga
//
LOCUS       DACEXQ010000127         7736 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.127, whole genome shotgun sequence.
ACCESSION   DACEXQ010000127 DACEXQ010000000
VERSION     DACEXQ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.127"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(26..616)
                     /locus_tag="IXH04_001874"
     CDS             complement(26..616)
                     /locus_tag="IXH04_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAQ1852470.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            1100..1327
                     /locus_tag="IXH04_001875"
     CDS             1100..1327
                     /locus_tag="IXH04_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAQ1852471.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            1343..2509
                     /locus_tag="IXH04_001876"
     CDS             1343..2509
                     /locus_tag="IXH04_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAQ1852472.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            2743..3786
                     /gene="hrcA"
                     /locus_tag="IXH04_001877"
     CDS             2743..3786
                     /gene="hrcA"
                     /locus_tag="IXH04_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAQ1852473.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            3880..4443
                     /gene="grpE"
                     /locus_tag="IXH04_001878"
     CDS             3880..4443
                     /gene="grpE"
                     /locus_tag="IXH04_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAQ1852474.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            4494..6323
                     /gene="dnaK"
                     /locus_tag="IXH04_001879"
     CDS             4494..6323
                     /gene="dnaK"
                     /locus_tag="IXH04_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAQ1852475.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            6474..7640
                     /gene="dnaJ"
                     /locus_tag="IXH04_001880"
     CDS             6474..7640
                     /gene="dnaJ"
                     /locus_tag="IXH04_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAQ1852476.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
BASE COUNT         2638 a         1336 c         1675 g         2087 t
ORIGIN      
        1 aggagtattt aattgaagag aatgattagg agaaaggatc tccgtacttt ttatagtaat
       61 catagaatcc aagtttgtat agacgaatcg ttttttctct tatttcttct gtttcaaaat
      121 ggactgcttg aagaatcaag tctgtgaatt ctataggagc tgttccattc gcagtaatca
      181 aattacgatc tttcacagct tgttgttcta taaaatcttc tttattctga taattttccg
      241 attctaacca aggaaattgc gcatttccgg tgtgtttgta accttgcaat aatccgctct
      301 gagctaaata atcaactgcc ccacaaatag cagctactgg tacgtctgtt tttaacaatt
      361 tatcgattat ttgatataaa tgatgatttt ttatgtccca tgaattgcca ccgatcataa
      421 taaaaaggtc aactttttca ggtatttgtt cgattaagta atcgattttt gtttttaatc
      481 ctccaatgga agtgacctct gattggatag aagcagtttt gttttgccat ttctcatttt
      541 ggtttagtgt gctagtaata taagcacctt cccaatcggc atattcctct aatagaaacg
      601 taatcgcagt tttcattttc aagctctcct ttactatcta taacaaggac aacagtttta
      661 acctacagac attcattaaa attgggtagc gacagcgaaa acaacaattc ttaagtttta
      721 gacagagtat acagatagtc aacttaatta aatacgaact aaataaaaat gacaggtaat
      781 catttttgtt taccagcatt ataacatttt gcatacatgg atgacaaaag aaccaattcc
      841 cacttgcttg agtacaatat taaaagaaac tggaaggcaa aaagtaatta tagaaacgaa
      901 aaataaatca tatcgggcac tcagacatga aaacaaccct agagatatat aactaggtaa
      961 ccagtttaat gaaagaaaaa atcatccaag aagtcgattc ttggattttt tagtggattt
     1021 gttcgccaaa tacgtttagt atgcgtatat gagaggttgt aagcaaagcg ttgtggtctg
     1081 agcacttaag gaggaatctg tgaaaatagc ttttcatatt tttgcagatt ttgacttaat
     1141 gccgaaggtc agctttgcga ttaccgttta tcgagaggct gtaaaagcac cgtgtagctc
     1201 cgatgacaat aagcaagcat ttttagagct aggtttcctt gattctaaat atgcggctta
     1261 tttcaatgag ttggtgcttg aacgcgattt taagaaggtc gaaaacaggc acgacttttc
     1321 gacgtaagga gaaagaattt caatgataga aacaataaat cccgcagaaa acaaggtttc
     1381 tgcgtacatt catatcccat tttgcgagca tatctgctat tactgtgatt ttaataaagt
     1441 ttttttagaa gggcagccag tagatgagta tgtggaaatg ctgctgaatg aaatgaaaat
     1501 aatgctagaa agtcatccga cagcgaattt agaaacactt tacgtagggg gcgggacacc
     1561 aacttcttta tctgctaaac aacttgatcg actgttatca ggtgctagag agatcctgcc
     1621 ttttcaaaat ggtaatgagt ttactgtaga agcgaatcca ggtgatttga cccgtgaaaa
     1681 acttcaagta atgaaaaact atggcgtcaa tcgtttatcc atgggagtgc agacatttga
     1741 taatcgctta ttgaaaaaaa ttggacgtaa acacaccgca gaagatgtgt atcaaactat
     1801 gcaattttta gaagcagaaa attttaccaa tgtaagcatc gatttgattt atgcattacc
     1861 gggacaaaca ctagaaggat atctggatac tttggaaaaa gctttggctt tggatctgcc
     1921 tcactattct ttgtactcat tgatcctcga aaacaaaaca atgttcatga actgggtacg
     1981 acaaggaaga ctcgaactgc ctgatcagga aacggaaacc agaatgtttg aagagacgat
     2041 tgaagcaatg gaaaagcatg gacgtcacca atatgaaata agtaactttg ggctctccgg
     2101 acatgaatct caacataacc tcatgtactg gaacaatgac cactattttg ggtttggagc
     2161 tggtgctagc ggttatcttg gagatattcg ttaccgcaac aaaggaccaa tccaacacta
     2221 tttacgccca ttacgagcaa atgaacgccc ggtattggaa gaagaaaaat tgacacgaaa
     2281 aaatcaaata gaagaagaaa tgttcttagg actaagaaaa aaaacaggga ttactaaaaa
     2341 acatttctat gatcgctatc aaaaaacaat tgaatcggtg tacggtgatg tgctaaggga
     2401 attagagcaa gaagggttac ttgttaatga agaagaccgt gtttatttga caccaagagg
     2461 gacctttttg gggaacaatg tgtttgagcg ctttttgttt gaaaaatagt ttaaagaata
     2521 tttgtttttt atctgaattt aaataaaaaa agttcaaaaa tacaataaag gactattgaa
     2581 acgttgatat aactggattt acatcaatta tttagaatta gcactcttgt gtttagagtg
     2641 ctaaaaaatg gagtctttct cttgacagga gaagggcttc ttgctatatt tataagtgga
     2701 ttagcactta gaatatatga gtgctaatga gaggtgagga agatgttaac aaagcgacaa
     2761 aacgacattt tacgtctgat catccaacac tatacgagta gtggcgtgcc cgttggttcc
     2821 aaaacattga tgtctgaggg agtcaaggcc agctcagcaa cgattcgcaa tgatatgaaa
     2881 gcattagaag atgaaggatt gctgttgaag actcactctt cttctgggcg gattccatct
     2941 gctttaggct atcgttatta cgtggatcat ttgttaaaac ctgctcgcgt agcagaagat
     3001 gatttgctgc tgattcgtca gtctctaggc agagaatttc atgaaatcaa tgagattatt
     3061 aagcaatctg ctgagatatt atctgagctg acaagctata cgactttttc attgggacct
     3121 gaaataaaag accgccgact gactggcttt cgtatcgtac ccttgaataa tcgacagatt
     3181 attgctatcg tcgtaacaga tatggggaat gtagaaagcc aagtgttcac tcttcccgaa
     3241 tatatgggaa gtcaggaact agaaaaaatg gtacagattg taaacgaccg tctagtcgga
     3301 gatcctctga tgacggtgta ccataaactt cgtacggaga ttccgatgat tcttcataaa
     3361 tatttccaaa cgacagaagg gatgtctcag ttatttgaca ccgttcttgg tcatgttttt
     3421 gaagataagg tttatgtcag tggacaaatg aatctcttgg attatgagcc ccgtcaagat
     3481 cttgatcagt tcaaatcgat gttttctttt atgaaagact cagacggact tacacaaatg
     3541 atcactccga tggatagcaa tattcatatc aagattggtg ctgaattagg caatgattta
     3601 ttgcagaata tgagtatgat tcaagcaagt tacgagatag atggtcatgg aagaggtaca
     3661 atagccttgt tagggcctac tagtatgcct tattctcgga tgttcggtct agtcgatgtt
     3721 tatcgaagag aactagcagc taaacttgct gattactacc gttcgcttga cttgtcaaac
     3781 tcgtaatgtg aataacgaag ctatttgcag ctgatcgttc tctctttctt agtaagagca
     3841 atcaaacagc tgactgaata atgaaaggaa gactgtatcg tgaaagaaaa tcaagaagaa
     3901 ctagataaag aaatgacaga tgctcagcca gaaccggaaa tcgatgtcga agctgcagaa
     3961 gattcgggta tttcagaagc tgaagcggaa gaattcgaaa cagcgaaatt aaaagctgag
     4021 ttagaagaaa tggaagataa atacttgcgg gctcgtgctg aaatcgcaaa tatggcaaat
     4081 cgaggaaaaa atgagcgtga gcaattacaa aaataccgtt cacaagattt agccaaaaaa
     4141 ttacttccat caattgacaa cctggaacgt gcattagcca cagaagtgtc agatgatcaa
     4201 ggggcaggtt tgaaaaaagg cgtggaaatg gtcttggaaa gtttgcgtaa cgcactggaa
     4261 gaagaaggaa ttgaaaaaat ccctgctaaa ggagaagcct ttgatcctaa tcttcatcaa
     4321 gcagtacaaa ctgttccagc gactgaagat acaccagccg atacgattgt agaagtacta
     4381 caagaaggct acaaattaca tgatcgcgtg ttaagaccaa ccatggtcat tgttgcacaa
     4441 taattgtgcg gatataaaac ttacactttc aattaaagaa ggagacgtta attatgagta
     4501 aaattatcgg tattgactta ggtacgacta actctgcagt agcagtattg gaaggaaacg
     4561 aagcaaaaat catcgctaac ccagaaggaa accgtacaac accttctgtt gtatcattca
     4621 aaaatggaga aatccaagta ggggaagttg caaaacgtca agctgtgaca aacccaaata
     4681 cgatttcatc aatcaaacgc catatcggtg aagcaggtta caaagtagat gtagaaggta
     4741 aatcatatac accacaagaa atctcagcaa tgatccttca atatatcaaa ggattcgcag
     4801 aagactattt aggtgaaaaa gtcgaaaaag cagttatcac tgtgccagct tactttaatg
     4861 atgcacaacg tcaagcaaca aaagatgctg gtaagatcgc tggtctagaa gtagaacgta
     4921 tcgtcaatga accaactgca gctgctcttg cctatggttt agataaaaca gatcgcgacg
     4981 aaaaaatctt agtattcgac cttggtggcg gtacttttga cgtatctatc cttgaactag
     5041 gagacggtgt ctttgatgta ttgtcaacag ctggcgacaa ccatctaggt ggggacgact
     5101 ttgataacaa aatcatcgac tacatggtag cagaattcaa aaaagaaaac agcattgact
     5161 tgtctcaaga caaaatggct ttacaacgct tgaaagacgc tgctgaaaaa gcgaaaaaag
     5221 atttatctgg cgtatcaagc acacaaatca gcttgccatt tatcacagca ggagaatctg
     5281 gaccattgca tttggaaatg acattgacac gtgcaaaatt tgatgaatta acagctgact
     5341 tagtagaacg tacaaaagta ccagtacgtc aagcgctaaa agatgcaggt ttatcacaat
     5401 cagaaatcga cgaagttatt ttagtcggtg gatctactcg tatccctgca gttgtagaag
     5461 cagtacgtaa agaaacagga aaagaaccaa acaaatcagt taaccctgat gaagtagttg
     5521 ctatgggtgc tgctatccaa ggtggcgtga tcactggtga tgtgaaagat gttgttttac
     5581 ttgacgttac accattgtca ttaggtatcg aaacaatggg tggagtgttc actaaattga
     5641 ttgaccgcaa tacaacgatt ccaacaagta aatcacaagt attctcaact gcggcagata
     5701 atcaaccagc agtagacatc catgtattac aaggtgaacg cccaatggct gctgacaaca
     5761 aaactctagg aagattccaa ttgacagata ttccagcagc tcctcgtggt attccacaaa
     5821 tcgaagtaac attcgatatt gataaaaacg ggattgtaaa tgtatctgcg aaagatctag
     5881 gtacacaaaa agaacaaaaa atcacgatca aatcttcttc aggtctaaca gacgaagaaa
     5941 tcgaacgtat ggtaaaagat gcagaagcaa atgcagaagc agataaagct cgtaaagagg
     6001 aagttgacct acgtaatgat gtagatgcat tgctattctc tgtggacaaa acattgaaag
     6061 aattagaagg caaagtggac gaagaagaag tgaaaaaagc agaaacagct cgcgatgaat
     6121 tgaaagccgc tgttgaagca aacaacatcg aagaaatgaa aactaaacgc gatgcattga
     6181 acgaaatcgt tcaaaacttg actgttaaac tatacgaaca agcggcacaa caacaagctc
     6241 aagaaaaccc agaagcagct caaggcggag cagatgatgt cgttgatgct gattttgaag
     6301 aagtcgatgg cgacgataaa taatcagttc actgaatttc gagagaaggc caaagttgct
     6361 actttggctt tttcttttga aaaatagttt gaagtatcgg tttttgtttc aaacaaaata
     6421 tgatatgata acaagcgacg tgaaaatgat gagaaccaat aggaggaaag cctatggcaa
     6481 caaaacgtga ttattatgaa gtcttagggc tctcaaaagg agcatcagaa gatgaaataa
     6541 agaaagctta tcgtaagctt tcgaaaaaat accatcctga catcaataaa gaacctgatg
     6601 cggaagaaaa attcaaagaa gtatcagaag catatgagat tttaagtgac ccgcaaaaac
     6661 gcgctgcata tgaccaatac gggcatgcag gcacagatcc aaattacggt gctggtggag
     6721 ctggtggcgg atttggtgga ttcggcggct tctctggtgg cggcttcggt ggttttgaag
     6781 atatcttcga ctctttcttt ggaggaggcg gtcggtcagt cgatccaaat gcgcctcgtc
     6841 aaggtgctga tttgcaatat acggtcgact tgtcttttga agaagcgatt tttggtgtag
     6901 aaaaagaaat caagtataat cgggaagaaa tctgtcatac ttgtggtggg aacggtgcaa
     6961 aaccaggcac acaaccaact acttgtcata aatgtcatgg tgcaggtacg attaatgtag
     7021 aacgccaaac accacttgga cgagtgatga gccgtcaaac ttgtgatgtt tgtcatggga
     7081 cagggaaaga aatcaaagaa ccttgtccaa cctgtcatgg ctcaggacat gagaagaaag
     7141 cacattcagt taaagtgaat gtaccagctg gtgtagaaga aggacaacaa atgcgtttag
     7201 ctggtcaagg agaagctgga gaaaacggcg gaccatttgg tgatttgtat gtggtcttcc
     7261 gtgtagaaga aagtgatatc tttgatcgtg aaggttctga aatttactat gaattgcctt
     7321 taagttttgt acaagcagct ttgggggatg aagtccaagt accaactgtc catggagatg
     7381 tgaaattgaa gatccctgct ggaacacaga caggtacgaa cttccgttta cgtggaaaag
     7441 gcgctccgaa gttacgtggt ggtgcaacag gcgaccaaca tgtcaaagta aaactgatca
     7501 caccgaaaaa tctaaatgaa caacaaagag aagcattacg tgcattcgct gaagctggcg
     7561 gcatcaaagt tgaagaacag caagaagacg gtttcttcga taagtttaaa gatgcttttg
     7621 gcgggaaaaa gcggaagtag tttttctagt aaaaacgcta agtcattaat aattaaaaac
     7681 cagacgaaca atcatatgtt cgcctggttt ttttgtagtt gtcatgtttg taccaa
//
LOCUS       DACEXQ010000128         7652 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.128, whole genome shotgun sequence.
ACCESSION   DACEXQ010000128 DACEXQ010000000
VERSION     DACEXQ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.128"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..1014
                     /locus_tag="IXH04_001881"
     CDS             <1..1014
                     /locus_tag="IXH04_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAQ1852477.1"
                     /translation="ELKPKHERQNFLVRDDRLDHAVAFLWKDPQTNETVGASYQGTFI
                     DYERFGERGTYKHIDKNSTANHGFNLKIGDPKQLKFFESSIDLLSYAALNRDQLNDTW
                     LVSMEGLKHHVISHYFGEAVSELRKKQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDP
                     FTNQKVRCERGIANDWQVPKEYKVIYEEVAKEEKITPEAIMAIHKTENNLQLTNQLVS
                     AHKVNASFGQQLSVNDSIEAINLKDICREVAKELKACERVDGTYDFDRFYQEKGDINA
                     QILFSYKAEQYYKGYKNHEHEFVPEVKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQ
                     ERE"
     gene            1025..3229
                     /gene="topB"
                     /locus_tag="IXH04_001882"
     CDS             1025..3229
                     /gene="topB"
                     /locus_tag="IXH04_001882"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ1852478.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            3470..4063
                     /locus_tag="IXH04_001883"
     CDS             3470..4063
                     /locus_tag="IXH04_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection protein"
                     /protein_id="HAQ1852479.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            4076..4975
                     /locus_tag="IXH04_001884"
     CDS             4076..4975
                     /locus_tag="IXH04_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAQ1852480.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            4978..5451
                     /gene="ssb"
                     /locus_tag="IXH04_001885"
     CDS             4978..5451
                     /gene="ssb"
                     /locus_tag="IXH04_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ1852481.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFANQNGNR
                     EADFINCVIWRKPAETMATLAKKGGLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSTTSPSPTTFEQRDTNNGLDASQNPFGGQSIDISDDDLPF"
     gene            5817..6077
                     /locus_tag="IXH04_001886"
     CDS             5817..6077
                     /locus_tag="IXH04_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852482.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDQFTRLMGI"
     gene            6522..6728
                     /locus_tag="IXH04_001887"
     CDS             6522..6728
                     /locus_tag="IXH04_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAQ1852483.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            6728..6979
                     /locus_tag="IXH04_001888"
     CDS             6728..6979
                     /locus_tag="IXH04_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852484.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTTETPLV
                     EDAPTMLFEDLVKIAGYELHYQENLETYENEYWLTELEN"
     gene            6995..7411
                     /locus_tag="IXH04_001889"
     CDS             6995..7411
                     /locus_tag="IXH04_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852485.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFIETKKAKTVSSEWLDYFENINKYGACYIMK"
     gene            7393..>7652
                     /locus_tag="IXH04_001890"
     CDS             7393..>7652
                     /locus_tag="IXH04_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852486.1"
                     /translation="MLHHEVIRRIQKDFSCMEEMKYFVQNVEEQLYLESETSQTKVTV
                     SHNRLDVYKRRPESLKKGAELLRLETEKYVMNVYLEKSELVE"
BASE COUNT         2834 a         1305 c         1574 g         1939 t
ORIGIN      
        1 gaactaaagc caaaacatga acggcaaaat ttcttagttc gagacgatcg tttagatcat
       61 gcggtcgcct ttttatggaa agatccacag acaaatgaga cagtaggggc gagttatcaa
      121 ggaacgttca ttgattatga acgttttggt gagagaggca catataagca tatagataaa
      181 aattcgacag cgaatcatgg ctttaaccta aagatcggtg accctaagca gttaaaattt
      241 tttgagagta gtattgattt acttagctat gctgcactaa atcgtgatca gttaaacgat
      301 acgtggttag tttcgatgga agggttaaaa caccatgtga tcagtcatta ttttggtgag
      361 gcagtatctg aattgagaaa gaaacaagcc tttcctcaat ccatcgagat ttgtgtggat
      421 aatgaccgtg caggacatat tttctacgaa aaagaacaat taatgggcgc agttgatcca
      481 tttacgaatc agaaagtacg ttgtgaacga ggaatcgcta atgattggca agtgccaaaa
      541 gaatacaaag tcatttatga agaagtcgca aaagaagaaa agatcacacc agaagctatt
      601 atggccatac acaagacaga aaacaatctg caattaacga accaattggt ttccgctcac
      661 aaagtaaatg cgtcttttgg tcagcagctt tctgttaacg attcaataga agctattaat
      721 ttgaaagata tttgtagaga agtcgcaaaa gaattaaaag cttgtgaacg cgtggatggt
      781 acgtacgatt ttgatcgttt ttatcaagaa aaaggggata ttaatgctca gattcttttc
      841 tcttataaag cagaacaata ctacaaaggc tataaaaatc atgaacatga atttgtacct
      901 gaagtaaaaa aggattggaa tgatcaattg aagcatgaaa tctaccaaca agagatcaga
      961 aagcaaaaac gtgccatgtt gtttcaacaa ggtagacagc aagaaaggga gtgaaatcgt
     1021 catgatgaaa accgttattt tagcagaaaa accgtcacaa gcgaaggctt atgccgatag
     1081 cttttctaaa gctacccgaa aagatgggta ttttgagatc caagatcgat tgttttcagg
     1141 agaaactgtg atcacgtatg gttttggtca tttagtcgag ttagattcgc ccgatatgta
     1201 cgatgaaaat tggaaacaat ggtcattaga gcatttgcct attttcccta cacattatca
     1261 ctatcatgtt cctaaagata agaaaaagca attcaaggtg gtgaaacagc aacttcaatc
     1321 ggccgacacc attattattg cgacagatag cgatcgtgaa ggggaactga tagcttggac
     1381 gatcattcaa caagcaggcg ccgatcatgg aaagatattt aaaagacttt ggatcaactc
     1441 cttagaaaaa gaagccattt atcagggatt ccagcagtta cgagatgcag aagagaccta
     1501 cccaaaattt gaagaagcac aagcacgcca gatcgccgat tggttgatcg gcatgaacgg
     1561 tagcccgctg tatagtttac tcttgcagca aaaaggcata ccaggaagtt tttccttagg
     1621 cagagtccaa accccaacgc tttacatgat ttatcaatta caggaaaaga ttagaaactt
     1681 ccaaaaagaa ccatattttg aaggaaaagc ccaagtaatc gctcaaaatg gcgcatttga
     1741 tgcgaagctt gatccgaatg aaacccaagc gacgcaagaa gcatttgaag attatttaaa
     1801 agaaaagggg gttcaattgg gaaaacagcc aggtacgatc caccaagttg aaaccgagaa
     1861 aaaaagtgcg gcaagtccac gtcttttttc tctatccagc cttcaatcta aaatgaatca
     1921 gctcatgaaa gctagtgcca aagatacttt ggaagccatg caagggttgt atgaagggaa
     1981 atatctaagc tacccacgaa cagatacccc ttatattaca gaaggggagt atgcgtatct
     2041 gttggatcac ttagacgaat acaaacactt tttaaaggcc gaagccattc ctactcctat
     2101 tcatacaccg aatagtcgat atgtgaataa taagaaagtc caggaacatt acgcgatcat
     2161 tcctacaaag acggtcatga cggcagcggc ttttgaacaa ttaagtccac tgcagcaggc
     2221 tatttatgaa caagtgttaa aaacaacggt ggccatgttt gcggaaaagt atacctatga
     2281 agaaacaacg atcttgacgc aagttcagca gcttcagcta aagacagtcg gaaaagtacc
     2341 agtagatcta ggttggaaaa agctgtttgg caaagagagc gacagaaaag aaaaggaaga
     2401 agaaccgcta cttcctaaag tgacaaaagg agaagcggtg acggtagatc tacaagtcct
     2461 tgaaaaagaa acgaagccac cacaacctta taccgaaggt acgttgatca cagcgatgaa
     2521 aacagctgga aaaaccgtgg atagcgaaga agcacaatcc atattgaaag aagtagaagg
     2581 aatcggtaca gaagcaacac gagcaaatat tattgaaact ttgaaacaaa aggaatatat
     2641 caaggtagaa aaaaataagc ttgtggtgac aaataaaggg attttacttt gccaagccgt
     2701 ggaaaaagag ccgctgctaa caagtgccga aatgaccgcc aaatgggaaa gttacttatt
     2761 aaagattggt gagcagaaag gaacacaagc tactttctta gctaatatcc aaaaatttgt
     2821 ctctcacttg ttggaagtcg taccgggaca aatccagtca acagattttg gatcaacgtt
     2881 acaagaggtg aaagcagcca gtgaaaaaca ggaagcaacg cgtcatttag gtgtttgtcc
     2941 aaaatgtcgg gaacaagaag tcttgcttta tcaaaatgtg gcggcatgta cgtctgaagc
     3001 ctgtgatttt aaattatgga caacgattgc caaaaagaaa ctcacagcca cacaactgaa
     3061 agagatcatt caaaacggcc gaacaagcca atcagtcaga ggactaaaag gtcagaaagg
     3121 ttcgtttgaa gcaacgatcg ttttaaaaga agactttaca acaggctttg aattttctga
     3181 aaagaaaaaa acgaactata agaaaagaac acgccgcact acaaaataat gaaggggtta
     3241 tcctatgcga aatacaacac acaaacaaca agaaatcgaa aatcaaaaag caaaaattcg
     3301 tttagaacaa gagaaattac gtgcaaataa aagaagactg caacaattaa agaaccagca
     3361 gcgcattcaa aaagaaatcg aattagatcg atagacgtta atcttgttac aaataaacta
     3421 ctatgatata tttgatgtag tgtatttgta acgaagggag gggaagtaaa tgcaagataa
     3481 actgaaagaa ttgttggcta ttcgcgatgg aaatttatct atgaaggaag ccaggaaatt
     3541 tggaatcgca gcaaccactg tacaaagatt ggtgaatcaa ggaaaattga tcaaagttga
     3601 tcgtggttat tatgtagagg atggtcatgg aatagatgat ttattttggc ttcagcaacg
     3661 attttccagg ggaatcttct ctcatgaaac gactttagat atttaccagc tttctacgaa
     3721 tatgccgaaa ttcattcact taacttttcc ccatggctat aacgttgatc gttcaattac
     3781 aaaagagcag cagttacaat ttcatttcgt gaagaaagaa gtttatgaac taggaaaagt
     3841 agagggaact tcctttcaag gaaatcctat tactatgtat gataaagagc gtacactttg
     3901 tgatatttgg aatccgagat atgaatttga atatgaaatg aaattagaag cattaaaaga
     3961 gtacatggaa gacccattga gagatccaag taaattaaga gattacatga caaaactaca
     4021 tgtagaaaaa gaaatgaaat accacatgca aagcttatat tgatggagga aaaagatgac
     4081 accgacacaa tttgcagcaa aaacaagaaa tttagcaaag agtacaggat taaatgcaca
     4141 gttagtgcaa cggcatttta tgatggatcg tctaattgaa caaatttctg aatcaaagta
     4201 caaaaatgac ttcattttga aaggcggctt tttattagga tcaaaatatg gaatcgatag
     4261 aaggacaaca attgatatcg atacgacatt tagaaatagc aaattaactg aagaaaaatt
     4321 gaagagcatt tttaatgatt taactagaaa acccacaaaa gaaggcattg ttttctctat
     4381 tcaaggaatg aaggaaacaa gagaagccga tttttatcct ggatttcaaa ttaaattggt
     4441 ggctaattta gagaaagtac gtgtaccgtt taaaatggac gtgacaacag gagattctat
     4501 ttatcctgaa gtagatacac actatcacca attaatgttt gaagataaaa aagtggagat
     4561 tccagcttat ccaacagaac aaattattgc agaaaagcta agtgctacgt tcagctttgg
     4621 cacagataat tcgagagcaa aagattacta cgacttattt accattccga aactagaaaa
     4681 tatcgatacg aaagagttat acaaaagtgt tcgaaataca tttacaaaac gtggggatac
     4741 aaaaccgttg tccttttatt atgaaagaga aatggataag atcagaggaa atgactattt
     4801 ggaaaagcag tgggaaaaat atcgctcagt taacacgttt gcgaattcca tttcatttac
     4861 agatacagtc aacgaaattg atcacctaat gaaaagtgtg atcaaaattg aaaatcaaga
     4921 aagaaaacgg cagtttaaaa gatcacaaca aatgaatcaa gaaatggaga gataaagatg
     4981 attaataatg tgacattagt aggacgctta accaaagatc ccgatttacg ttatactgca
     5041 agtggcacag cagtagcgac ttttacccta gccgtcaacc gaaatttcgc gaatcaaaac
     5101 ggcaatcgag aagccgattt tattaactgc gtgatttggc gcaaaccagc agaaacaatg
     5161 gcgacattag ctaaaaaagg cggcttgatc ggtgttgtcg gacggattca aacacgaact
     5221 tatgacaacc agcaaggaca acgtgtgtat gtgactgaag ttgtagcaga taactttcaa
     5281 ctattagaaa gtaaagcagc gacagagagc cgtgcgcacg ctgatcaatc aaccaccagt
     5341 ccaagcccca ctacctttga acaacgggat acaaacaatg gattggacgc ttcacaaaat
     5401 ccatttggtg gacaatcaat cgatattagc gatgatgatc taccattcta agtagcattt
     5461 tctattggtt gcatttgcta cggtttatgt tataatttag tcaacaaaaa gaattttcaa
     5521 tctataacca aaaaccacta gctggcgggc tagtggtttt cttttacgtt tctattataa
     5581 gaattaacgg gtaggcttac tgtctttctt ggcaaattta caatcaagcc atttgcagat
     5641 atagtgcgcg gctacttctg ccatgattgc aaaaacaaaa tcaaaaagaa ttttcaaacc
     5701 tataacctcc tccccttgcc agattaggcc ggggtagcag acagcaagac tattatagca
     5761 taattgaaat attcaaatga ttggattttt gctataattt tagcgaggtg attgtgatga
     5821 acaaatatgt tggtttgctt gataaaatcc gagtaataaa aacgcaaccg ttattggttc
     5881 gctttacttt acaaacgatt catgaatcga ttaattgtgt agttgcagat attgaaatta
     5941 tcgataaatt gctaattatg gatgatggaa aatacaatat tgccgtcaca ggtcacttca
     6001 ataaacgaaa ccagctagtt attgctagca tgtatgttag aaatcccgat cagtttacgc
     6061 gattgatggg catataaaaa aagaagagtt gcaatcctaa tgtaggactg cagctctttt
     6121 tgttttacat agaaacaaga aaaatgaaaa ggccttttta ggaaggcgcg agcgccttct
     6181 caattgaaaa acctctctca ctccaaccgc caccgccgtt cgagaagaaa ggaagtgaac
     6241 gttgcacgtt cactaagata aagggtcttg caaagcaaga caaaatagaa aaaacttcct
     6301 ctcaccaaaa ggttcgttcc tgttcgttat ctctttattg atttgccaga ccggcaacaa
     6361 gttgcctttt atcaccaaaa agcgacaagg acaagggtaa atggcacgct gcgcgccctt
     6421 gtccttgtcg cttttcgttg attcattctg gccaacaaat caataaagaa agaactaacg
     6481 aaaggaagta aaacctttcc taagaggagg cagaatcaat catgcgaaag aaatttgatt
     6541 attggggtgt tcccttcagc gaattattcc caaactatca tgcaccacat actgtcgaat
     6601 gcgattgtgg agaaagagcg aaatgtatca aaagctatcg tttgtatcaa tgtccaacat
     6661 gcgggaaaaa atataccttg tcttatggcg attatatttt gattgatgag aaaggaaaga
     6721 aacgttgatg aaaacacaac acaaattatt gactgtaaaa gaagtcgttc aaattttggt
     6781 ggacaaagaa gtcatttctg aaaaagtagc agaagcttta cttattccgc ggtactggac
     6841 gacagaaacc cctttagtag aggatgcacc gactatgcta tttgaagacc tagtgaagat
     6901 tgcaggatat gaactgcatt atcaagaaaa cttggaaact tatgaaaacg aatattggtt
     6961 aaccgaatta gagaactaaa aaggagaaaa aaatatggct caaaattact atgacgaatt
     7021 tgtcaaatta cctttggata aaatggcaca aaaaatggag gatatgacat tcctatacaa
     7081 tgagacacgt gttccaaaaa aacattataa ggaaagattg tcggtagctg tggaagaaat
     7141 gattgaatca ggtgtcgaaa tgaatctcat tgcgacgtat tatcgaacac tggaagaact
     7201 gaaaaagcaa aatgcaaaat ggtttttcca agccttacta tgcttggaag taggcgtcaa
     7261 accaagcaca atcaaacctt ctgaatacca agctttagaa ctaacgtatg cgaaatttat
     7321 cgaaacgaaa aaagctaaga ctgtttcaag tgaatggtta gattattttg aaaatatcaa
     7381 taagtatggc gcatgttaca tcatgaagta atcagacgga ttcaaaaaga ctttagttgt
     7441 atggaagaaa tgaagtactt tgttcaaaat gttgaagaac agttatattt ggaaagcgaa
     7501 acgagtcaaa caaaggtcac agtcagccat aatcgtttag atgtgtataa acgtcgtcct
     7561 gaatcattaa aaaaaggagc ggagcttcta cgactagaaa cagaaaaata cgtcatgaat
     7621 gtttacctag aaaaaagtga gttggtcgaa aa
//
LOCUS       DACEXQ010000129         7586 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.129, whole genome shotgun sequence.
ACCESSION   DACEXQ010000129 DACEXQ010000000
VERSION     DACEXQ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.129"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(125..415)
                     /locus_tag="IXH04_001891"
     CDS             complement(125..415)
                     /locus_tag="IXH04_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852487.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(533..793)
                     /locus_tag="IXH04_001892"
     CDS             complement(533..793)
                     /locus_tag="IXH04_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852488.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(930..1343)
                     /locus_tag="IXH04_001893"
     CDS             complement(930..1343)
                     /locus_tag="IXH04_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAQ1852489.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1799..2284)
                     /locus_tag="IXH04_001894"
     CDS             complement(1799..2284)
                     /locus_tag="IXH04_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852490.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2423..2668)
                     /locus_tag="IXH04_001895"
     CDS             complement(2423..2668)
                     /locus_tag="IXH04_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAQ1852491.1"
                     /translation="MFYHLRKENSNENLDAIALTLLIVGGLNWLLVGLFELDLVAMIA
                     GGSTTIFAKIIYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2861..3661
                     /locus_tag="IXH04_001896"
     CDS             2861..3661
                     /locus_tag="IXH04_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1852492.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3680..5548
                     /locus_tag="IXH04_001897"
     CDS             3680..5548
                     /locus_tag="IXH04_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1852493.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRSYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5541..6032
                     /locus_tag="IXH04_001898"
     CDS             5541..6032
                     /locus_tag="IXH04_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAQ1852494.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6020..6574
                     /gene="srlA"
                     /locus_tag="IXH04_001899"
     CDS             6020..6574
                     /gene="srlA"
                     /locus_tag="IXH04_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAQ1852495.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6592..>7586
                     /locus_tag="IXH04_001900"
     CDS             6592..>7586
                     /locus_tag="IXH04_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAQ1852496.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2704 a         1213 c         1440 g         2229 t
ORIGIN      
        1 ataacataat aagaaagtaa agtggattga tttgagatct ttttatactt caaaaaagat
       61 aacacccaac tttcaaaaat atgttttgga agttgggaca aacgagttaa tacattttaa
      121 tacattattt tttattcaaa aaaaatagca caatggcaaa gatcgtaaga gagatcataa
      181 gccccaatga atgactaaaa agaaaagcag aatcacatgc catatcatcc catatttctt
      241 caagaaaata aataagcggg aaaaagggaa ggaatagtag aacatcgatc cagcaccatt
      301 tttttcttct tttgaaataa cataaaacaa taaacatgaa cgtgcaggga agtaaaaaca
      361 aagctttact cataactaac aaaataccag ctccttttat ttttttctcc cccacattca
      421 aagagtaaca atggtatgta cttaataata gaaaaatgga tcaacttgta tgaataacag
      481 ctgaaaagat ctgcaccaaa taaaaaactt ccagccaaaa ctgactggaa gtttattttt
      541 ttctgaaata ttctaacaca aatatgaaaa agatggccga agctaataag ccgatttcgt
      601 agcaaaacaa tgagatacca gagtcgtaat aataaaatcc tctaatctta tggataaaat
      661 agatcatcgg acaagcagat aagagtaagt atttgttagg ccttttgaat aagtaaaaga
      721 aagcactggt cattatgata aatggtatcc aaaacaaaag tttacttaag acaatcaaat
      781 gatcaactcc catatttttt accaagagag atcataacat ctttatcgta aaaacaaaat
      841 atttttttgc tcattttgaa aatttaaaca tactcgttct tatccaaata tataaaaatc
      901 gcccctcatt catcgagagg cgataagttc taatttaact caattttttt tgtttctttt
      961 ggctgataag ctgttttagg taataccaat ttcagcacac cgtttttaaa ggttccagtg
     1021 attttttctt catcaacatt aggaagatag aatgaacgtt tgtaagagac cgaatgtctt
     1081 tctttccgta catagttgcc atcttcttta tcttctgtat gggactcatg attggcggag
     1141 atcgtcaaag tattatctga atattcaact gtcgtgtctt ctttgtcaca gccaggcata
     1201 tcagcagtta gtttgtattc tttttcattt tcgactaaat ctacttttgg ataacttgaa
     1261 acacctaaaa aatcattgaa tgctggacta aacacgtcat tgaaatcagg gaacatgtcg
     1321 cgaatgctag gtacatttgc catttttgat catcctttca ataagtattt tttatttatc
     1381 cctatttacg taataactct attttgaaaa aaatgcaagt ataatggtta gctttgtgaa
     1441 tatttcatct aatacgatac aaaggctatc tttctaattt gaaaaaggga tcattaaaat
     1501 aataagaaga aaatatgtca aaaggtaaaa aagtaaattc ttctatccaa atatagaaaa
     1561 attgaatcta gaaaaccgcc cgacaataaa tttcattaac tcagttgacc gaaaatgaag
     1621 tagataccaa aaatttatca agagagcaag tagagaagtg agtatattca taagagaaat
     1681 agctgtaacg aattattagg ttaatgcaaa tgcttattta tactccgtat aactttctca
     1741 gaaaaaatga taccaataat gacttgctag tactgtaaat ccgtataata tcatctaatc
     1801 aaaatggtgt tccatttgca tctgtcatcg taatcgatcc ttcgggtgac actcgataaa
     1861 aaccaacgct tccagatcct ccttgggaac ggatttgttt gctggaagct gtgaacaaat
     1921 aatctcgatc aattttttga taaaagctaa taacaatatc gtcactttgt aattcggaac
     1981 ggtgggcaat aagaaataga agtgctttgt cagagttttc taatggaaga gaaggtacag
     2041 tgctttcttg catttcagat gttgaagaaa tgctaaacga ctgaatactt gattggctat
     2101 gttgcgaatc tacattacca gtagaagtat tagtaatgga agtagtagaa gtacttttag
     2161 ttgaaccaac ctcagttgtt gaaatggctg ctgaagattc tttaacagaa ttctttgttt
     2221 tatatccaca ggcagaaaaa agtattacag ttcccaacaa gcctgcgtaa atccctttat
     2281 gcatgatagc tcctcctaaa atagattatc tttattcagt atatcactta aacaaagaaa
     2341 agcagatggt ttaaaatgaa aaaaccttcc caataaaggg aaggttcatg aagataataa
     2401 cttttttaat aattatagct ttctaatatc tttcatcaac ttttctagta atcattggga
     2461 aaaatttcaa gcagtagatc gcacaaatac ctacgacgat ataaatgatc ttcgcaaaaa
     2521 tagttgttga accaccagca atcatagcta ctaaatcaag ttcgaacaag cctaccaaca
     2581 gccagttcaa ccctcccaca atcaataaag ttaatgcaat agcatctaag ttttcattag
     2641 aattctcctt tcttaaatga taaaacaatt atatatcaaa aagagaaaca ttgcttatat
     2701 aacgctcagg aattttagca atctatgcaa aaagatgaaa attttttttg ctcttccatt
     2761 agtacacatt ttttagctga agctgtaaat ttcatgtctt aatttttttt agtgagcttt
     2821 tatactggat ttgtaagaaa aaagaaggag aggatcacat atgtcggatt ggttggatat
     2881 tgcaggaaaa gtagtaattg taactggagg ttcctcagga attggaagaa gtatcgtcga
     2941 aaatttgttg aagcagaatg cacaagtagc gaattttgat gtaactgaat gccgtataca
     3001 gcatgaaaac ctactatctt taaaagtcga tgtgtcttct aaaacggaca tagaagaagg
     3061 gatatataaa gtaatgaaac atttcaagac gatcgatggt ctagtcaata atgcaggaat
     3121 aaatattcct tctcttctaa tagatagaaa tcatccaaaa agcaagtatg aattatcaga
     3181 acaagttttt gataagatga tcgctgtcaa tcaaaaaagt gtatatttga tgagccaagc
     3241 agttggaaga atcttggtgc aaaagggatc aggagtgatc gtgaatcttt cttctgaatc
     3301 aggattggaa ggatcagaag gacagagctg ttatgctgct acaaaagcag cgatgaacag
     3361 ttttactcgt tcttgggcaa aagagttagg aaagaggaat gttcgtgtgg taggtgttgc
     3421 accagggata ctggaagaaa ccggtttgag aacacaagaa tatgaagaag ctctttctta
     3481 tacgagaggt ataagcgtcg aacagctgag aaacggttac agtcgaacaa gtacgatccc
     3541 tctaggaagg agtggtaagc tgcaagaagt ggcggatcta gtctgctatt atctttcaga
     3601 aagatcaagt tatatcactg gagtcacgac taatatttca ggaggtaaaa caagaggata
     3661 aaggataggg gggacgcacg tgacgctagt aaacagatgg taccaattat tgaaattact
     3721 agtcactcac aagaaagttt caatccagga agtgcaaaaa gaaatcaaaa ccagtaatca
     3781 aaccataaaa aaagatattg gggagttgaa tgaagaaatt gccggtattg caaaaatcat
     3841 ccaaaaaaac aaacattttg aacttgaaat ttatgatttt gatttttttg atgagatcat
     3901 gcaaggaaaa ttgaaaacag ccagtgattt caattcggca tccaaaagaa tcgcttattt
     3961 actaaatagg ctgatcgaaa cagaggattt cttacgtatg gatgatttgt ctgaagaagt
     4021 cggtgtcagc agaggaacag tggtcaagga tctaaagaca ttaaaagaga tgatcaatga
     4081 ttttgatgta aagattacag gcacaccaaa taaaggacta agaataatag gagatgagct
     4141 agaattacga ctacttttat tttattttgt ttctccctac ttttcagaga cgaaaacaga
     4201 acagtttcta gaagaaaaac aagcaatcaa acattttcag aagaagacga atgctagtaa
     4261 acaagaaatc acccttttaa cgaaagttat cggtatttct ttgaatagaa tctctagtaa
     4321 ctatctgttg aagaagccca tcaatcgtta cagtgggtgt tttgaatata tccaagaatt
     4381 caatgaactg atcaatcagt tggaagaaat ctatgaacta actttgagcc aatatgaaaa
     4441 agattttatc agttttcctt tgaacatcat caacacaagt ataacgatga taaggaaagc
     4501 taactctgat ttgcgcttat atttcgaccc catgatgatg cgtattcgac gattagatct
     4561 cattgatctg gatgagtcct tcttatatca agagatgaaa cagcacctct ctttattgat
     4621 aaatcgggta atttttcgtt atcgattgac tgatttattt tatggtgaga tagaaaaaaa
     4681 gtatcctttt tcttaccaaa tcgctagcga atgcatacaa gcattagaag ctatcttgca
     4741 acataagatc tctaaagtag agatcagcta cctaacgttg tatttggagt tagcatttcg
     4801 atatcaaaaa gagcattttt cctctaaaaa agtagccatt gttcatacga caggaaaagg
     4861 aacagcatta atgatccaaa gacaaatcaa aaggatattg gggagggata tacaaataac
     4921 acatgtttca gaagaacaat atgaaaaagt agatctaaac aaatatttcg ctgtttttac
     4981 aacgattccg ttaaaaaatg taaatgaaga gacaccggtg atccatttga ctaatctttt
     5041 tgatgactct tggttaaggg cacaatggca aaaagcaaaa aaaaagcgat tagccgggtt
     5101 tcattctact gtttttttct atcaaatatg gaaagaaaaa aagacgtatc aagaatattt
     5161 aaacaaatta gtcgctcctt tgatcagaag ctattatgtg gatgaagaat ttgaaaaaag
     5221 gatcttgaaa agagaatatc agcaaagcac atttgttaac aaagaatttg cttttcctca
     5281 cacaaccaac cagctatctc aacacattgt tttatcagta gggctaataa ctgatcaagg
     5341 tttagcagaa acaggaggaa atggagttat ttttttattt gccatacctg aagaaatgac
     5401 ggctgaagtt gagaatgaat tatttgagct atacgatatc ctatttgaac tcataaaaat
     5461 gggaatagat gagtttattg aattagcacc gacacaagaa tcatttttaa aactaatcaa
     5521 aagtaaagga gagagacttc atgaatgata tgtatctatt catagttttc gcagcttcag
     5581 cttatgtgct gcaagcagtt atcagttgga agcaactaaa gcatttcaat caaacatata
     5641 gcgaactgcg aagaaaagga aaagtagcga ttggtaaacg acctgggaaa atccgttcag
     5701 gcacgattgt tatgctagct atagacgatc atgatcaagt tttagaagtc agaaaaatgc
     5761 aaggggttac atctttggca aaatttcatt cattacctgt ctatgcgggt gaagatatcc
     5821 attacatcga tcgttaccat ccctcagttc gagcggaaga tcgtttgaca caagcagctt
     5881 tagaaaatgc cagagaagta tacttacgaa tagaggcagg aaatgaaaaa agtgaacaac
     5941 cttcattgcc ttttattgct atgctcaata attttcggat atggaaaagt acgatcaaag
     6001 aaaaaaggag tgacaaacga tggatatcat aacaaaattt gctgagggat ttatgaggct
     6061 gttccaaaca ggtgcagaaa cattcataag ctggatgaca ggaatcgtac cagttgtctt
     6121 gatgctgatg gttgcaatga atacgctgat tgctttttta ggtgaagacc gagtaacgaa
     6181 agtagccaaa ttatcagcga aaaatccgct tactcgttac cttgtgttac cgttcttatc
     6241 tgcttttatg ttgggaaatc ctatgtcatt tacaatggct cgtttccttc cagaatacta
     6301 caaaccaagc tattatgctg ctcaagctca attttgtcat acgagcaatg gtgtattccc
     6361 ccatattaat cctggagaat tgtttgtatg gatggggatt gctcaaggga tcgaaacctt
     6421 agggctaaat tcgatggagc tggctatccg atacttgctt gtgggactgc tgatgaactt
     6481 tattggtggt tggataactg attttactac tggatttgta tgcagacagc aaggaatcgt
     6541 tcttagcaaa aaagtagagc tttcagttga ttagagaaag gagagtttta aatgtatggt
     6601 gatattaaga tcgtaagagg aaacggcggg tatggaggtc ctttgattat taggccgaca
     6661 aaagataaac acaagttcat ctatgtcaca ggtggaggtg agaggccaat gatcgtagat
     6721 aaaatagaag aactgacagg tatggagtct atcaatgggt tcaaaacatc aatacctgat
     6781 agtgaaatcg cgcttgcaat cgttgactgt ggagggactc ttagatgtgg aatttatcct
     6841 aaaaaaggga taccaaccat caatatcgtt cccacaggaa aaagtggccc attagcacaa
     6901 tatatcactg aagatattta tgtttcagca gtagggttag aacaaataca gccactaatg
     6961 gaaactgatg ataacaaaga aacagtaaat actgcatctc cagataaaaa gtacaaatat
     7021 gatacttcta aaaagataac cgagcaaaaa gcagagcagc aaaactggat agcaaaaatt
     7081 ggaatgggcg cgggaaaagt agttgctaca ttcaatcaat cagcaagaga agcagtagat
     7141 acgatgatcc ggacagtgat cccttttatg gctttcgttt cccttttgat tggtgtgata
     7201 caaggttctg ggatcggtaa tgtatttgct aacattatgg cacctttagc aggaaatgca
     7261 gttggattaa caatcattgg ctttatttgt tcactgccat ttttatctcc tttgcttggt
     7321 ccaggggcag ttattagcca agtgattgga acattgatcg gtgtagagat tgggaaagga
     7381 aatattcctc cccaattagc attaccggca ctatttgcta tcaatactca aaatgcatgt
     7441 gattttattc cagtcggtct aggtttggaa gaagcaaaag ctgagacagt agaagtaggt
     7501 gtaccatcag ttctttattc aagatttctt aacggcatcc cacgagttat cattgcttgg
     7561 gtagcaagtt ttggattata tagctg
//
LOCUS       DACEXQ010000130         7567 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.130, whole genome shotgun sequence.
ACCESSION   DACEXQ010000130 DACEXQ010000000
VERSION     DACEXQ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.130"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            249..1034
                     /locus_tag="IXH04_001901"
     CDS             249..1034
                     /locus_tag="IXH04_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ1852497.1"
                     /translation="MRESQGTPFEWKAGDSYVQLINIDKKDFLILKNLLGKLKYSSYI
                     KVVNYFVNTIFSEFSEKEKNEISEQIRKNMSYCSFSSADCKEKNEEYVVQLLTQIYFF
                     SRIFPYDTTHLTIRATYFAQQMQHRQIRLYAGFRAELQFYSTLLNQNLIYYLSSYIFW
                     MLIGNPEIHHYTSDKKILIISDLSLRHSQYIEEYISDILAVHKIHSETTAITEDQLSK
                     YNLLEYDLIVTNQPILNAHVPHILIDDSVSFANEEELIRLFEL"
     gene            complement(1107..1754)
                     /locus_tag="IXH04_001902"
     CDS             complement(1107..1754)
                     /locus_tag="IXH04_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1852498.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(1762..2448)
                     /locus_tag="IXH04_001903"
     CDS             complement(1762..2448)
                     /locus_tag="IXH04_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1852499.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(2454..3527)
                     /locus_tag="IXH04_001904"
     CDS             complement(2454..3527)
                     /locus_tag="IXH04_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ1852500.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            3749..4423
                     /locus_tag="IXH04_001905"
     CDS             3749..4423
                     /locus_tag="IXH04_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1852501.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            4428..5768
                     /locus_tag="IXH04_001906"
     CDS             4428..5768
                     /locus_tag="IXH04_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1852502.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(5917..6903)
                     /locus_tag="IXH04_001907"
     CDS             complement(5917..6903)
                     /locus_tag="IXH04_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAQ1852503.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         2380 a         1401 c         1283 g         2503 t
ORIGIN      
        1 aaagctattt attaaaagtg atcgttgacc aaggttttca gttaactagc cttaaaagtg
       61 agctccagtt gcgtatcttt gttacctttt tctttttaag ttactacgga agacgcacgt
      121 tgccatttcc aatcgaaaaa aagaagatca aagaaattat ggtcaagaat aattgtcaga
      181 tgaatttgtt tcaggaagaa agcccatatg agcaatcttt ctttattatg tattttgttg
      241 tgcatctcat gagagaatcc caaggaactc catttgaatg gaaagctgga gactcttatg
      301 tacagttgat aaatatcgat aaaaaagact tcttaatctt gaaaaatctc ttaggaaaat
      361 tgaagtacag cagctatata aaagtagtga actattttgt caatacgatt ttttcagaat
      421 tttcggagaa agaaaaaaat gaaatcagtg agcagatacg aaaaaatatg tcctactgtt
      481 cgttttcatc cgctgattgc aaagaaaaaa acgaagaata tgttgttcaa ttattaacgc
      541 aaatttattt tttctcccgt attttcccat acgatacgac tcatttgacg atccgtgcaa
      601 cttactttgc acagcagatg caacaccgcc agatacgtct atatgctgga tttcgtgcag
      661 aacttcaatt ttattccact ctactgaatc agaatctgat ttattattta tctagctata
      721 tcttttggat gcttatcggt aatcctgaaa ttcaccatta tacttctgat aaaaaaatac
      781 tgattatcag cgacttaagt cttcgacata gtcaatatat tgaagaatat atcagtgata
      841 ttcttgctgt gcataaaatt cactctgaga caactgctat tactgaagat cagctgtcga
      901 aatacaatct tctcgagtat gatctgatcg ttacgaatca acccattttg aatgcccatg
      961 ttcctcatat attgattgat gatagtgtgt cattcgcaaa tgaagaagaa ctcatccgct
     1021 tattcgagtt atgaataatc aaaaaacagc tatgtataaa ccaccctgat aaaaatttca
     1081 ggacaaatac acatagctgt ttctttttat tgtattcctt cagcaatcca acgagattta
     1141 tagcgaaagt cttttaacaa ctcttctttt gtccaattat ttgcaatgag atccgtcaaa
     1201 gtgttcatta cgagggtcat ggaaaagtat aggaaactaa ctgcttcttc cgacgattga
     1261 atggagaaat gattgattct taataattcc agccattttt cgttcattgg agaatcagcc
     1321 gatagttctc tacgtttggt tgtctgctga tcatttcgct gggttaacaa gtataacctt
     1381 tgccaataga tactttcgtg atctaagtcg cagcaccact ctaaatattt ttcgatcccc
     1441 aaaaagaaat gttgtttgtt ttcgctaatg aattttaaga tatcttgatg gactatgtga
     1501 gaactgcttt tgatcgtata caaataagca tcttccaaat cttgaaaata tttataaaaa
     1561 gcaccgcgcg acatccccat cttgtcaacg atttctgaga ctttgacttg gctgatgtgc
     1621 tgtgagcaaa atatctccag taataggtca tcgattctct gtttctttgc gttcgataaa
     1681 ttgaaaaaag tttcttttgg catccacatc tctcctttat ctctgattat acgtggatag
     1741 cccttcattg tcatgaagga ttcattcctt ctttttctct tcttctgtca agtacccatc
     1801 ttccattcga taaacgcgat cactccattg aatcatacgg acatcatgag taaccataat
     1861 agtcgctttt tgtttctcat gagcttcttt aactagtaat ttgacgactt catatgcatg
     1921 atctgtatcc aaactagctg ttggttcgtc tgctaagatc aagcttggct cattatataa
     1981 agcgcgagca attgccactc tctgtctttc tccaccagaa aggtctttag gataactatt
     2041 tttaagtgac tggatatcta aagattgcag caaggaatcg atttttgttg aatcttcttt
     2101 ttttgaagta acttggtcaa ctagttcaaa ttgttccttt accgttaaaa aaggaatcaa
     2161 attagagctt tggagaataa atcctatttc tttaaagcgt aaatctgctc gttttttttc
     2221 tggaagctca gttagtgagt gaccgttgat cactatttct ccggaagtcg gtgtttgcaa
     2281 tcctcctgaa atggtaagga aagtactttt acctgaacca gaaggaccaa tgatgctgac
     2341 gaattctcct tcctttacat aaaaatcaat tgctttcaaa gctgagacct ttgtatgacc
     2401 agaaccaaat gttttggtaa cttttttcat cgttaatatt tttttcattt ttattaacct
     2461 cctattgctg taattgggtc tactttggtt atcgtacgta tcgaaaacaa tccgcctagc
     2521 attgcgacaa tgatcaaaat cccactatat agcaaccatt gtgaccaaaa aacagcaaaa
     2581 ggcatcgcgc ttggtaaaat cagactagtc aaataagcaa acaaaagagc aagcagaacg
     2641 cctaccaccc caacaataaa ggactgtgct actaaagatt tagcgatgaa gaaatttttg
     2701 atcccttgtg ctttcattac tccaaaaata gcagtcttct gaagtgtcat cacgtacatg
     2761 aaaataccga ccacggcagc aaccactaag aacaaaaagt atatcatcgc atttaaggtc
     2821 atattttggg cagaatagcc aggaatactt tcaatgaatg tcccaatagt taataactgt
     2881 agacccttcg atgctttttc tttcgaaatt ttagctgaat cctttattac gatcccattg
     2941 atcggctgtt cttcttcaga agcaaacggc tgttgtccat acttcaaagc agtccacgta
     3001 tctagggacg catagatgac gggagaaact gtgtaataag tttcaggaaa gataccgaca
     3061 atcgtcaaag gatcgtcgta actgccaatt ttgatcgtat ctccaatttt atagcctgaa
     3121 tcagccagat tttgagagat gatgatttca ttctttgttt taaattcatg ccctttcgtg
     3181 agttttggta ataaaaatgc ttctttcgtt gtgccgaaaa ctgtaacatt ttctttgttt
     3241 tttcctttga ttgctccgct gtacaatccg attggtgctt tctctcctcc ttcaatgtat
     3301 ttcagatctc ctcttgttaa ttgtgaagct gcaaatactt gatttgcatc ctccgacaaa
     3361 acaatctctt gtgcgtccca atcatcgatt gcttttttaa actcttctga aagtccattc
     3421 gctagtcctg acaacatgaa tacgacatac gcaatcaaga acataatacc gacgatcaag
     3481 ccatatcgta atttagaata gcgaatttct ttgatagcta gaaacatttg tttttcctcc
     3541 ctatgataga ttgatagtga caaaatgtca cttgttgaat agtgtaactc taaagtgaca
     3601 atttgtcact tgtgttgttt ttttcttatc aataaattgt ctaagacatc tttcgataaa
     3661 accttaacat gagtcataaa aaatttattt aagccttatt tatgctttcc atgataaact
     3721 aaagtgacca aatcaatgag gtgggaaaat gattaaacta ttagtagtag aagacgataa
     3781 agtattatcc aataatataa aagaaatact cgctgcaatc ggcgagataa cacaagttta
     3841 tgatggtagt gaaggactgt tcgagttgga aagtggagtt tatgatcttg caatcttgga
     3901 tttgatgatg ccggaaatga atggatatga agtgttagca aaaatacgga aagaaaaaat
     3961 acagactcca gtattgattt tgactgcaaa agatagtttg gaagataaga ttgaaggatt
     4021 tgaacgaggt gcagatgatt atctaacaaa acctttctat cgagaagaac tgatcatgcg
     4081 cgtgaaagca ttattgaaac gctcgctagg tctctttgat gaaaatgttc tgaagtataa
     4141 ggatatcagt tgtgatttaa aaagcaatga agtcttgtat aaagaacaag ttttgcccat
     4201 acaaggaaaa gagtttgaat tgctcgttta ttttttacaa aataagggaa tcattttgac
     4261 aaaagagcag atatttgacc ggatctgggg atttgattct gatacgacga ttacagttat
     4321 tgaagtttat atgagcaact tacgaaaaca tctgcgaagt actggattgg ataaagaaat
     4381 caaaacctta cgtaatgtcg gttatatact gcaaagggtg tgatgcaatg aatggagata
     4441 ttacacgaaa gcaacgtagt cgcttttttg cgacaaatgt agcagcattt gcttttatct
     4501 tttttctttt gggattcatt actttacaga tattaaacca atctgcatac agagaaacag
     4561 atctgtcatt agagaaaata aattttgatt ctcgaacagt acaattagag atacaaagat
     4621 atcagcaagg cgatcccttt ttaaacccga ctcaaacgga acaaaacaga acaaacatgg
     4681 aacagccatt aatggaacca tcaaatgacc gttttaacac tcaagtgatc ttgtggtcta
     4741 aaaatggtga gatattgaat aaagcagcta ttggtgggcg tttcaaccaa atcaaagaat
     4801 taaaaatgga tacgaaaaat ctgaatacca tccaaaatat tgaattaaat gaagacagtg
     4861 gctcatctgc cctctctttc cgttcgatta cgaaaaaagc ggagacgaat caagatggcg
     4921 ttgcctacat ccaaatttta gaaaatacaa atcaaatcaa gaattcatta gcgacttttc
     4981 gcacgattat gattttgtgt atgattgttt tttggattct ctctattggt atcagttatt
     5041 acttgtctag tctaagcatg cgccctatta ttctttcttg gagaagacaa aaagaatttg
     5101 ttgagaatgc ttctcatgaa ttgcgcacac cgttaacgat tattcaaaat agtttggaac
     5161 atctttttac caagcctaat catactatta ttgaagaatc cgaaagcatc gctcaagcat
     5221 tgtcagagac gaggcgcctc actggattga cttcggattt gctgacaatc gctcggaatg
     5281 attctgatca gcagctgctt tcaaagcaaa tgatcaatac acaagaatat atcgaacaac
     5341 ttgttaaacc atttcaagag atggccatca tagatggaaa agagtttatt ctccataacg
     5401 atgcaacatt agaagtacca attgatgaaa aaaaaatcca tcaagttttg gttattttgc
     5461 tggataatgc attaaaatat acgaatccag aagataagat cattttggat tcatctgtta
     5521 accataaaga atggctgatt cacgtaaaga atactggccc ttctatcagt gaagaagatc
     5581 aagcaaggat ttttgatcga ttttatcggg aagatcaatc tcgggcgaaa gagactggcg
     5641 gatacgggct tggcttggca attgcaaaac aggtaatcga acagcatcat ggacagattt
     5701 ctgtatccaa ttatcttcca caaggagtcg attttaaagt ccaattgccg ttgaagccta
     5761 aaaaataatg cttatcccta aaaaagggct gtgacaaaaa tcttgtcaca gccctttttt
     5821 gttgcgaata aacggtgttc cagaagtagc ttcttcggaa ataagccgaa atttctttaa
     5881 gctgaccact tctgtcacaa cctctttagc ttacgcttac gattttttat tgcggtcttc
     5941 aatgtgtaca tcaccgcttg tgtcaatatc caattcttct cggctaacat cttctgtcac
     6001 tttttcaaca tcttcatgtt cttctttatg aatcccaact tcttcacgaa taactgtatg
     6061 tttgcttaca tctacttgct cttctttgat tgggatagta atcgtgtcat cttccaattt
     6121 ttcgtctgtt ccctgtgaag attgatctgt tactggtttg cgttcgatga cgatttcttc
     6181 acgtttgacc ggtacttcta ccgtttcagt atcgttgacc acatgtttgt ggatgtcgac
     6241 ttctccagtt gtcacatcat gtgtatttac atctaatttt tcttctttta gttgaattgt
     6301 atcatcatct agatttggtt tgcgatcgat gtcttcagtc ttgcttgtac ccattgtatc
     6361 agtctcgcca ggtacgtcca tcggatcagt tcctggtgtc atattcaatg gatccgttgt
     6421 attttgaaag cctgtcgtgc cacctacgtc cattggatct gttccagtgc cagtttctaa
     6481 tccagtcccc attgtgcttg ctgggctaac agtatcttgt gtatccattg tatcttctgt
     6541 tgtttctggc tgacgatagc cttttactgt gatgacaaat tttccatcag aaatatctcc
     6601 tctatattga tataaaggat cattttctgg tgtagccgtc tcagaatcat aggtatcaaa
     6661 agaaaaagta tcttttattt tttcccataa agaacggtct tcttcaccat ccatccgatt
     6721 ttcatctgtt tcaacatcta taccagacaa tccgtcaaat cctagtctag atgctgcttc
     6781 atcagtagta taaataacga tatcatctcg ttcataccct tcgttacgca accgttcaat
     6841 tactgataat gcttcttccc tcgtactata gcttccgatg accgttaaat ttttctcttc
     6901 catgtaaatt cctcctagca aatagattta tgattggtat attgttactt ataaatttat
     6961 tctataggag acgattgttt tctacaaata tttagattgg aatttgatta aaacaattta
     7021 attgaaattt gaaaacattt atgttaatga aagtcatatt ctaataaaat gtcatttgtt
     7081 tctatatgaa tttttatgaa aaatttcata taatttccac tcgttgtgat ggtttaattt
     7141 atccaatcca tcaataagca aacaacttat ataaaaaagg attaattctc aatataattg
     7201 ctcctaccat agaataaatc ttcatgtaaa gtaatgaagt accaactatt tctaatactt
     7261 ctttaagaat tatcccaaca atatcgttag acaaaccatt attgaataaa tggagagcca
     7321 atcgattctg aaaaaatgtt ttctaatttt tcggaaataa aagaaagagc ctcctgccat
     7381 tagaacgaac caaatccatt tcattgctca tgaagtaacc acatagctaa aaagaggata
     7441 aatcaattaa aagatcaaaa gaatgactgc actaataaaa taactaaaaa tgtatcttct
     7501 tacttcatat cttttagcta gtacttagtt aaaaatttgc ttcctaagat caagacaaac
     7561 tgtctat
//
LOCUS       DACEXQ010000131         7537 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.131, whole genome shotgun sequence.
ACCESSION   DACEXQ010000131 DACEXQ010000000
VERSION     DACEXQ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.131"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            16..2181
                     /locus_tag="IXH04_001908"
     CDS             16..2181
                     /locus_tag="IXH04_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1852504.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            2485..3318
                     /locus_tag="IXH04_001909"
     CDS             2485..3318
                     /locus_tag="IXH04_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAQ1852505.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(3420..5120)
                     /locus_tag="IXH04_001910"
     CDS             complement(3420..5120)
                     /locus_tag="IXH04_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852506.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            5627..6787
                     /locus_tag="IXH04_001911"
     CDS             5627..6787
                     /locus_tag="IXH04_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1852507.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            6803..7246
                     /locus_tag="IXH04_001912"
     CDS             6803..7246
                     /locus_tag="IXH04_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1852508.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
BASE COUNT         2519 a         1252 c         1610 g         2156 t
ORIGIN      
        1 aaggagctaa acggaatgaa aaagatattt agttttgagt tttggcaaaa atttggaaag
       61 gctttaatgg ttgtggtagc agttatgccg gcagctgggt tgatgatcag tatagggaaa
      121 tcaattccct tgatcaatcc tgattggacg ccgcttgtca caacgggcgg agtaattgaa
      181 aatattggtt gggcaattat tggtaatctt catattttat ttgctttagc aataggagga
      241 agttgggcaa aagaaagagc aggcggagca tttgcagctg ggatctcatt tattttaatc
      301 aatcggatca ctggggctat ctttggtgtc acaagtgaca tgcttgcgaa tgaagatgca
      361 tttacccaca ctctgttcgg tacgaagatc atggttaaag gattttttac aagtgtattg
      421 gaagcaccag ctttgaatat gggagtattc gtagggatca tcgctggatt tgtcggggca
      481 atggcttata ataagtacta taattataga aaattgccgg atgctttgtc atttttcaat
      541 ggaaagcgct ttgttccgtt tgttgttatc ttatggtcaa caatcgtggc attgattctt
      601 gctgtggtgt ggccaaacgt acaggctgga atcaataatt ttggtttatg gattgcagaa
      661 tcacaagaaa gcgcaccgat tctagcaccg tttttatttg gaacattaga acgtttacta
      721 ttgccatttg gattgcatca tatgctgacg attcctatca actatacaca gcttggcgga
      781 tcgtatgaga ttttatcagg cgcacaggcg gggacacaag tatttgggca agatccgtta
      841 tggttggctt gggccactga tttagtgaat cttaaaggtg caggggatat gtcgcaatat
      901 gagtttgtgt tgcataactg gacgccggct cgtttcaaag taggacaaat gattggttct
      961 tcaggtatct tgatgggaat ggcttttgcg atgtatcgta atgtcgatcc tgataagaaa
     1021 gcaagatata aatcaatgta tttttcagca gcgttggctg tctttctaac aggtgtaaca
     1081 gaaccattgg agttcatgtt tatgtttgct gctgtgccat tatatgtcat ttattcagtg
     1141 atccaagggg ctgcatttgc gatggcagat atcttgccat tgcgggtcca ttcatttggg
     1201 aatatcgaat tgctgactcg gacaccgtta gctatcaaag cgggtctagg tggagatctt
     1261 atcaacttcg tgttgtgtgt gatcgctttt ggtgttgtta cgtatttctt agcaaatttc
     1321 ttgatcaaaa aattcaactt tgctacaccg ggacgtaacg gaaattacga caatgacagt
     1381 gaagaaaccg ttgtatctaa cagcggaaca ggaacagcag atcagcaagt ggtccagatc
     1441 attcatttgc tgggaggaaa agaaaatatc aaagatgtcg atgcttgtat gactcggtta
     1501 cgcgtaagtg taaatgatcg agaaaaagta gggactgaag aagcctggaa acgagcaggg
     1561 gcgatgggat tgatcgtcaa agacaatgga gtccaagcag tttatggacc aaaagcagat
     1621 gtcttgaaat cagatatcga agatctgctg caatcaggag cagagattcc agagccagaa
     1681 atggtttcta caggaaatgg acaagcaggt agtaagcaat acttaggtat agaacaagaa
     1741 ctagtttccg cagcttctgg agaagtgatt cctttatctg aagtaaaaga tccggtattc
     1801 tcacaaaaaa tgatgggtga cggctttgct gttattccaa catcaagaga agttgttgcg
     1861 ccgatcgctg gaaaagtgac aagtattttc cctagcaaac atgcaattgg gctggaaacg
     1921 aaagatggta tcgaagtttt gatccatatg ggaatcgata cagtccaaat gaatcagcca
     1981 gcttttgaga ttgctgtcaa agaaggccaa gaagtaggaa caggaacgaa attagctgag
     2041 atggatcttg atgtgatcca aaacgaagaa aaagatccaa cgatcatgat cgtatttaca
     2101 aacgataaag tagaagaagt agtgatcaag caattaggta cagtgacagc tggtaaagtg
     2161 atcggctcca ttaaaatgta aggagaggtt gtgaaaaagc tgtcctgcat caagtcataa
     2221 gaacagccaa acaaaaatag ctcctcctat ttttcgtttg gttgggctta ttaccgagaa
     2281 gcagcagttt gaatactgtt tagtggagag gtcgtgaaaa agctgccctg caccaagtga
     2341 taaaaacagc caaacaaaaa atagctcctg gtccattgag cttattactg aggagttagc
     2401 aggagaacac tatttgctgg agaggttgtg acaaaaattt tgtcacaacc cttttgtttc
     2461 gaaatgaaga caaggagaaa accaatgaaa ttcatgacac tcaacacgca tagctggctt
     2521 gagccagaac cagagaaaaa attgcaagaa ttagcagata agatcctgtt agaagattat
     2581 gaagtgattg ctttacaaga aatcaaccaa ttactggaat ctgaagaagt agaacctgcg
     2641 aagctaatga agttctgtcc ggtaaagaat caagtaccta tccgtaaaga taattttgcc
     2701 taccgtttag ttcagctttt aaaggaacac ggaaaagagt atttttggag ttgggaaatg
     2761 agccacatcg gttatgataa atatgaagaa ggaaatgcac tgcttacgaa aaaaacattg
     2821 gaaagtcagg tccttaccgt ctcccaattt caaaagaaag aagattatca gacacgcaag
     2881 atcttgattg ggaaaacaaa gatagatgat caggacattt ttgtctcgag ctgccacttt
     2941 tcttggtgga cagataaaaa aagtggcttt tattttgagt ggaaaaactt ggaaaactac
     3001 tttttggaaa ctcgcgtacc actttttttc ctaggtgatt tcaataatcc agtagactct
     3061 caaggttatt atactgtaag agaaagttgc cttcttcttc aagattcgta tgttgtcgcg
     3121 aatgaaaaag gaaaagcagc tactgtggaa aagaaaatag atggttggga acaaaataca
     3181 gaaaaattgc gtatagattt tattttcgtc cctgaaggaa tgcaggttaa aaaatatcaa
     3241 cggatttttg atgggatcga tagtccaatc atcagtgatc actacggggt ggaaatcgaa
     3301 ttagatgtga acgaataaaa aatataacat ataacataaa aaaactgatt aaaataaaac
     3361 tctccaaacg attataccgt ttggagagtt ttttgatcgg atgtatttat cccttttgcc
     3421 taagcacgta aaagcttgta tctatgataa ataaataaac atagcaaggt aaaccagcca
     3481 atcaaagtaa gagaaagaat gaaatagaaa tagattggcg tttgataatg aagcgtgagc
     3541 tcgttgattc ctttttgctg cttcacgatt ggggtcccga tatctgttac ttcataatca
     3601 gtcaatgttt ttccattttg ctgtagcaca gtccgatcgt agacgataac agggatatga
     3661 acagttttgt ccccagtgtt tttccacttg attgtcaaaa gaccgcctgg atgtgttttt
     3721 ataaaagttg tttcgttttc tagtatttct tcgttatacc ggtcgtattt attgtctttt
     3781 gtctccttat agattggcag atagtcaggt gtggtttttt ggaaaacgaa caagctggta
     3841 gacatatctt tgctgaagaa tgttttcctt gcttcttccg gcgtatcgaa tagataagta
     3901 tgtcgactga caaatgtttc attttcccac ttttccagat gctggtataa gttctggatc
     3961 gtctgtgctg aaaaaataag aatgccaaat atacttaaaa gacggaacca cttttgttca
     4021 gaccactggt tcagtgaaag ggcaaggtaa agtagaagca atagcgtaaa tggcacgaaa
     4081 aatcgaaatg gaaactggat caaactaata aaaggcacat ttttttcgat caatgtcgtc
     4141 catggaatca agctagtaga aagaaaaaag aaaacaagac ttgtgtacaa cacgattttt
     4201 tggaataagg tgttcttttg ttgaaagaaa ataaaaagca aaaaacctga taatatcaat
     4261 ataggaaagg ccttagggta atggagccaa taactgccat ccagatctat cgccttttga
     4321 ctcatttctc tgttaacaaa gggaggaaga atcttattcc cactgtaaac aatagctaat
     4381 cctgcccaaa tatttgctgt cagaacaaaa taaagcaaga ccgacttaat cagttgaagc
     4441 agtgcttttc ctttttcagt ctggttaaaa aagtaataaa gataaaaagg gatatacatc
     4501 aaagctaaca tgatcgaaga cagtaaatgg atctgtgtca taagtcccac tgagacagcg
     4561 actttgataa cagggaactt tttatgaagg acgaaatcag caatagggat catacataaa
     4621 ggataaaaac aagtccccca gctagtgaat ccttggttga tagaccagta ttgaatagaa
     4681 tacgtactca tgaataaaac ggctactggt acacttaccc aatttcgaac attgacatat
     4741 ctcagtaaaa tcagcataga aaatcctgaa ataagataaa gaatgaaatt tgaaagaatc
     4801 tgataatgaa accagctacc tgcaagcaac acgatcaatc cttgtaaata agcgaaaaat
     4861 gggccgtaaa cggcattcac gatcctgcca ctttgctgga atccataaag ggaaggaaaa
     4921 taatggaaat ccccatattt aatttgttgc gcggcatcat agaaccgatt gtaatggaat
     4981 ggggcatcag cacccaaaat tagtttatgt gtgaataaat gaggcatgag aaatgccaaa
     5041 gcaatgaaaa aaattgctaa aaatgggcca tattttttca attgtcgaac tcctttgcaa
     5101 actttagata aattcatcat atatcctatg cgagaggtag gcaagaaatt agaaaattca
     5161 tgaaaatatt tatgaatttt acaagaaaaa cataagtgaa agttctaagg agagaggatg
     5221 cgacataagt tttgttgcat ctccttgttt tgaaacagaa tttttcttaa tactcgaaac
     5281 aaagtagctt tttcatgacc tctcggtaaa cggtgttcaa tcagctgctt ctcggtaata
     5341 agctcactag cctagaaaat atgagaagct attttctgac tagcgctctt attactcaaa
     5401 gcataacgca gttttcacaa cctcttcctt aaacggtgtt caaaagcttg catctgcggt
     5461 aataagccca acccaacaaa aaatatgagt agctattttt ttgggctgta cttattactt
     5521 gatgcaggac agctttttca caacctcatc ggctttcatt ttctattttt ttcctgaacc
     5581 catataataa aagaaagaac aaaaaaactt gcaagggagg acacatgtga agcagagtaa
     5641 gcaaaaacag tctcgtcgac ttttgtttcg tttgttttta ttaatatgta tcgtcgtagg
     5701 gttactgatc gctttatatc cattttatgt ggattcttta aatagtttta ttgatcaaaa
     5761 acgtatcgaa caagtacaaa aacggaccgc agctgaaatt gaagtgcagc gaaagaaaat
     5821 ggaggagaaa aaccaacgat taactgacca aggattcaat cctggtactg atcctttcaa
     5881 tgaaaagaac agaaatgaaa gtacaacatc cagccaatta gaggaatggc tgatcggaag
     5941 tatcaatata cctaagatcc aattaaatat ttcactttat gatcggctca atggtaggat
     6001 ccttgaaaat ggagcaggcg tcttgcaagg aacctctttc ccgcttggtg gaaattcgac
     6061 gcattcagtg atctctgctc acagcggttt acctaatcga cggttattta ccgaattaga
     6121 tcgtttagaa aacggagaca cgtttatttt gactgttttg ggggagaaat tggcttatca
     6181 agttaaaaat atacaagtgg tactgccaga tgacacgtct gttctaacga tttcagaagg
     6241 agaagactta gttaccttgc ttacatgtac gccgtatatg atcaacacgc atcgtttgct
     6301 agtgacagga catcggattc cttataacga atcggtaaaa aaagaagaag gaaaaggaaa
     6361 tcaggaaaga accatgcggc aatggtttat tcttgcagga acgataatag ctgtagtcat
     6421 actgttagtg tttatcggac gactaatcta tcagtataga ttaagtaaaa aagtgcttga
     6481 tttttcattt attattagtg attcgtcagg gaaaccggta aatggtagtt cattcatttt
     6541 gaaacataag aaaaaaacac ttactagaaa tggtgtgccg ttttcagtac aaagcgacca
     6601 tcatgggaaa gtcaagctgg atcaattgcc tggtggaact tatcgaatcg tttcagctga
     6661 tgatcctaaa gttgctgttt cttttggtat tagaaaagta aaacaagaga agatatactt
     6721 ttttgagggg cgaaaacttg taaaagagct tcaaaaaaat ggtttttatt ttaaaatcaa
     6781 tgaataagta aggagagaaa aaatgatccg cacaacaaca aaattgtctg ccaaagaact
     6841 ggaatctgtt ctatcgattt ggctgcatgc taacttggat tctcatgaat tcattccatc
     6901 aacttattgg gagaatcaag tagaatctgt taggaaagct ttaccagatg cagtgattta
     6961 cttagcagaa atagatcaag aaattattgg ttttgctggg gtgattgatc agtttatcgc
     7021 aggaatcttt gtgaaaaggg aataccggca acaaagaatc ggtacgcaac taatgaaaaa
     7081 aataaaagaa accaataaaa cactgacttt gacagtctat caaaaaaatc aatcagctat
     7141 tcatttctat ctttcacaag gttttacaat tcaaacacag caaaaagatg aagcagtaaa
     7201 tgaggaggaa cttgtgatga aatggaatca aaacgctgat ttttgatgaa cagaataacg
     7261 aaataatgct cccagcctaa ttttttgctg ggagcattat ttcattttga agtcactaat
     7321 ttaactgtgt gaatatgcag ttgttacaat ctttgataaa cggtgttcaa tcagctgacc
     7381 ctcggtatta gctcaaaaaa cggcaaaata tgaggaacta tttttccatt tgtttctcta
     7441 atactcaggt caaaacgctg atttcacaac ctcttgataa acggtgttca aacggctgct
     7501 tctcggtaat aagcccacta gcctagaaaa tatgaaa
//
LOCUS       DACEXQ010000132         7505 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.132, whole genome shotgun sequence.
ACCESSION   DACEXQ010000132 DACEXQ010000000
VERSION     DACEXQ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7505
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.132"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..1316
                     /gene="glmU"
                     /locus_tag="IXH04_001913"
     CDS             <1..1316
                     /gene="glmU"
                     /locus_tag="IXH04_001913"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAQ1852509.1"
                     /translation="LYKVLHPVSGKPMVEHIINRVSETKPDEVITIVGHGAEQVKAQL
                     GERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISGDTPLLTTETLNNLFEYHQGK
                     NASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDATPEEALVQEINTGTYCFDNEA
                     LFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQTDDFEESMGVNDRIALAKANE
                     IMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIEAGVQIQGNTVIGSDCVIGSH
                     SKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRPKAEIGENVHIGNFVEVKNAQ
                     IGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNKHHTTVGDHSFIGSSTNIIGP
                     VEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYAKKLPYLN"
     gene            1410..2381
                     /locus_tag="IXH04_001914"
     CDS             1410..2381
                     /locus_tag="IXH04_001914"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ1852510.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      2387..2489
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2555..3124
                     /gene="thiT"
                     /locus_tag="IXH04_001915"
     CDS             2555..3124
                     /gene="thiT"
                     /locus_tag="IXH04_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAQ1852511.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(3205..3978)
                     /locus_tag="IXH04_001916"
     CDS             complement(3205..3978)
                     /locus_tag="IXH04_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852512.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            4245..5945
                     /locus_tag="IXH04_001917"
     CDS             4245..5945
                     /locus_tag="IXH04_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAQ1852513.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            5915..6133
                     /locus_tag="IXH04_001918"
     CDS             5915..6133
                     /locus_tag="IXH04_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852514.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            6153..7043
                     /locus_tag="IXH04_001919"
     CDS             6153..7043
                     /locus_tag="IXH04_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAQ1852515.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            7061..7345
                     /locus_tag="IXH04_001920"
     CDS             7061..7345
                     /locus_tag="IXH04_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852516.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
BASE COUNT         2553 a         1292 c         1567 g         2093 t
ORIGIN      
        1 aattatataa agttttacat cccgtttctg gtaaacctat ggtagaacat atcattaatc
       61 gggtcagtga aacgaaacca gatgaagtga ttacgatcgt tggacacgga gcagaacaag
      121 ttaaagcaca actaggagaa cgcagtaaat atgcgttaca agcagaacaa ttgggaacag
      181 gacatgctgt tttgcaagct gcttctttct tggaaggaaa aaaaggaaca acgttagtta
      241 tcagtggcga tacaccatta ttgactactg aaacgctgaa caatcttttt gaatatcacc
      301 aaggaaaaaa tgcaagtgca acgatcttga cggcacaagc agaaaaccca acaggatatg
      361 gacgaatcat ccgtgaccat attgggatcg tagaaaaaat cgtggaacaa aaagatgcaa
      421 caccagaaga agctcttgta caagaaatca atacaggaac ttactgtttt gacaacgaag
      481 ctttgttcga tgcgttaagc aaagttggaa caaacaatgc tcaaggtgaa tactatctaa
      541 cagatatcat cgaaatctta aaagaagaag gccatacagt tgctgcttat caaacggatg
      601 attttgaaga atcaatggga gtcaatgatc ggatcgcgtt agctaaagca aatgagatca
      661 tgagaaagcg aattaaccag atgcatatgg tcaatggcgt aagctttgtc gattcagcaa
      721 cgacatacat cgatgcaggc gtagaaatcg gtccagacac attgatcgaa gcaggtgtac
      781 aaatccaagg aaatacagtc attggttctg attgtgtcat cgggtcacat tcaaaaatcg
      841 tagacagtag gattgaggat cacgtcgtca ttgaaaactc agtcattgaa agcagccatg
      901 tcaagaaaca tgcagatgtg ggaccttatg cacatttacg accaaaagct gaaattggcg
      961 aaaacgtcca tatcggaaac tttgtggaag tgaaaaatgc tcaaatcggc aaaggaacta
     1021 aagtcggtca cttgacttat gttggtgatg ctacgctggg agaagaaatc aatgtcggat
     1081 gtggtgttgt cttcgtcaac tatgatggaa agaacaaaca tcatacgact gtcggcgacc
     1141 acagctttat tggttctagc acgaatatca tcggacctgt cgaggtagct aaaaacagtt
     1201 cgattgctgc cgggtcaaca attacagaca atattccaga atatgcgctg gcaattgcga
     1261 gagcaagaca agtcaataaa gaaggatatg caaaaaaact cccttatcta aactaagact
     1321 tttgcaaaat cacttgattt atttaaaaaa tatgactact atttattagg tagaattcta
     1381 gagttgtgaa aaagaaagtg gaggtcccca tgtcaaaaca ttactttgac ccaagattaa
     1441 aaatttttgc gttaaattcc aatcgcccat tagcagagaa aattgcagca gcagtcggcg
     1501 tcgagttagg aaaatcatct gttactcaat ttagtgatgg agaaattcaa gtaaacattg
     1561 aagaaagtat tcgtggatcg catgtctatg ttattcaatc aacgagcagc ccagtcaatg
     1621 ataacttgat ggagttgctg atcatgattg acgcactgaa acgagcaagt gcaaaaacaa
     1681 tcaacgtagt catgccttat tatggctatg cgcgtcaaga ccgtaaagca cgtgcacggg
     1741 aaccaatcac agcgaaatta gtagcaaaca tgattcaaaa agctggtgca acaagaatgc
     1801 tgactttgga ccttcatgca gttcaaatcc aaggtttctt tgatattcca gtcgatcatt
     1861 tgatgggtgc gccacttatt gccaattatt tcttagaaaa aggaatcaaa ggcgatgatg
     1921 ttgtcgttgt ttcaccagac catggtggtg tcacacgggc acgtaaatta gcggaatttt
     1981 tgaaatctcc gattgcgatc atagacaaac gccgtccaaa agcaaatgtt gcagaagtaa
     2041 tgaatatcat tggacaagtc aaaggcaaaa cttgtgtact gatcgatgat atgatcgata
     2101 cagcaggtac gatcacattg gctgccaatg ctttgaaaga agctggtgca actagtgtgt
     2161 atgcttcatg tacacatcca gtattatccg gccctgcttt acaaagaata gaagattcag
     2221 ctatcgagcg tctagttgtt acagactcga tctatcttcc agaagatcgt aaaatcgaca
     2281 aaatcgatga agtaagtgtt ggcggactaa tgggagatgc aatcaaacgt atccatgaaa
     2341 acaaaccagt tagtccattg tttgaaacaa aaaataaata ataaataaac tcacaaaggg
     2401 gagtccaatt gggctgagat tgaatctatt tctaaaccct tcgtacctgt atcggttatg
     2461 cgagcgtagg aattgtgaat aaacagctgt ttgactattt tcgaaagctg cttcctcctt
     2521 tgtgactttt ttattcaaag gaggaagttt ttttatggga aaaaatagag tttgggtaga
     2581 aggaacgatt gttgcagcgt tagcgatggt gttgtcgttg attccgatcc agatcggcag
     2641 cagtttttct atttcattag ggcagattcc attgactctt tttgcgttaa gaagagggac
     2701 aaaggcaggg atgctggcag gtttcatttg gggcatccta cattttccgt tgggacaagt
     2761 ttattattta agtgtcgttc aagtgttgat tgagtacccg attgcctata cttttgcagg
     2821 cgcagcaggg ctcatggctt caaatgtcca aaaaggctat gtagacgata atacgaaaca
     2881 aattagaaaa agcatcgtgt ttggtgcatt gattggcgca ctttccagat atttttggca
     2941 ttttattgca ggagtggttt tctggggagc gtatgcacta tgggggatga atccttggct
     3001 cttttctttc gtaatgaatg gtgccagcgg gttgagtaca gctattgtaa cgatcatcgt
     3061 tttattaggg atccaacaaa cgactccttc gatttttgtg ccagataaac gacaggagct
     3121 gtaaataaaa aagaagcaag aaacttgaga cagaatcatg tcctagtttc ttgtttcctt
     3181 tttatttttt gataagaaat agatctactt caatggcaga taagaaagat cggcatcttc
     3241 taccaattgc cagtgtcgat cggtgacaga gtagattaaa gtagcgatga tttcctcttc
     3301 tcgataccgg gtgcctttta atttccaaat ataatcttca atcacataga ttgggatacg
     3361 atatttacca tcgatcaaac caacttgatc aaaagaaaaa atgatccctt cctgcatcaa
     3421 attacgcatt tctttcaata attccacact gattttttca acaggtgttt cttttttgaa
     3481 aaaagatttt tttacattga tcttatctga caagatcgaa cttaatttac tagcaaaaat
     3541 cctataaaat tcatctaaat agcgataata aatccgattc agctgatcat taagatcgaa
     3601 gtatacaaaa ttattctgta gtttataaaa aaacggcgaa tgaagatgtg ttttcatatg
     3661 accaaaatat aataattctg aaatttctaa aggtgtcaat tctttcagca tcgttaaatc
     3721 agtaaagtcg atccacttca attcatcgaa tccacgcctg ttacgagaag aggaaaagaa
     3781 acgctgaata ttttctgtac cacggattac tttcagccct gtatgggatt catattctcc
     3841 agcagaagca gatggatcaa gcaagagcaa gttttgagga aaatgcacga tactgtttgc
     3901 aaagtcagta gcagtcagcc cttttgttaa aacagcatta cttgtggtat caatatgtac
     3961 ataaactgac tcagtcatca tgttcctcct cttatttcct cttaatctct tttattttac
     4021 aacatttgca ttaaaaaagc ttgtttcttt tattttaaaa ttccattaca aaatcacgtt
     4081 ttcattgaaa acactctcat tttcaattaa tttttgtgat ttttgtattt ttttatttaa
     4141 aggaggtttt atgaattgtt ttcttatgga atctacaaag tcgaatacga gactcatgac
     4201 ctttttatga cgtactggta tacttgaaga aaaaggagag cgcgatggag ataaaaaaag
     4261 tttacttttc acagaaaatg tcgctgtatt cgcctatcca gcaagcaaaa caaccccttc
     4321 cattttttca aagtcatcaa gaaagtagag acgctttttg gcgttctgca aaaaagcaat
     4381 gtgcttttaa agcagtatcg aaaaaatata ccgtctattt tactattgct aaaagtagag
     4441 gagatgttat ttttaataat ctgttgattg aaggtgaaac ttttgaatgg aaaaaatttt
     4501 ttcggtatat ggaagcttgc gctcgttttt ttattaaaga aaactgttgt tttttatttc
     4561 aatctcctct atcagaagaa tggttgcgta tttttcataa aaacggctat caaggaacca
     4621 cacaattaaa taaaaagctg gtctatcata cagcacttgt tttaggtgga ggaggagcgc
     4681 acggtgctta tcagattggt gtgtggcagg cgctgaagga acatgatatc aactttgaga
     4741 ttatcacagg tacctcggta ggtgcgttga acggcgcatt gattttacaa ggagatatga
     4801 aaaaagctgt gaatttatgg aaaaagctgt cgacaagaca agtgcttgcg ttaccagaaa
     4861 tggctgcagt tgaagacctt cgtgaacgct tctatcgaga aagacgccaa atgacgaaaa
     4921 cagcactaat tgaaggcggg gtttcttctg ctccattgga aaagatggtc aaagacaatt
     4981 tggatttgtc cttactcaaa cataatccca aaatacgttt atatacggtg tctactaaac
     5041 tgccagaact agcggaagta gtgactgata tccaacagac aaaaacagaa gaaataccag
     5101 attggatact cgcctctgct tcattttatc ccgctatggc ttatcgaaag atcggaaaaa
     5161 ataaatatgc agacggtggg tatcgcaaca aaatacctat tgatattgcg attaacaaag
     5221 gagcaacaga ggcatttgtc gtagatgtgc aagggccagg cccagcgaaa aaaattcgta
     5281 tgccagacat ttttatccat tggaaatgtc aaacattgtg gacactaggc agctttctgc
     5341 tttttgatag ccaaaggaac caattaaatt tacaattagg ctatctggaa atgaaaaaac
     5401 gattaggcta ttactatggg aactggtata catttcattc tgttaaacct gcaagaacat
     5461 actggcgagg atttttgtct tacttaacaa aagaaattca gttagaatta tcttttttca
     5521 aaagcataaa attttggcaa aaactgcgta atttatataa gaacagggtc gtaccagaga
     5581 catgcggatt ggcgatgcta gaacttttag caaaaaaaca ttttttactg cccaatgaaa
     5641 tctatcaagt agacatgatg attcaaatga tctgccaaca agatataaaa agtatgccgg
     5701 atgatttatt ggcacaaatt ggtcaattaa gtactgaaga atggcgaagg tatcgaaaat
     5761 accagcaaaa aatacaaaat gagcgcacga aaactgttta ttttgattat ctgctacaag
     5821 gaaaacgcaa ggatcgtttg cagcatgaat tgcttaagca accagttgac acattactga
     5881 tcctatattt atattatctc aaggaggaac aaccatggca caagaatttt catatgaaat
     5941 cgtagaagaa atcgctatat tgtcagaaaa caacaagggg tggcgtaaag aactgaacct
     6001 agtcagctgg aatggtcgcc cgccaaaatt cgatcttcga gattgggcgc cagaccatga
     6061 aaaaatgggg aaaggcctga cattgacgaa cgaagagttt gaacaactgc aaaaagcaat
     6121 agaaaatatg taatgaggaa ggaccctgtc gaatgaaaag tatgactggt tttggcaaag
     6181 ccatccgcga aacaaaagcg tatcagttag atattgaaat aaaaagtgtc aaccagcgtt
     6241 ttctagatgt tcaaatacga agtcctaaaa tcctgaactt tgttgaaaac gattttcgtc
     6301 agcttattaa acagcagtta agccgcggtc gaatcgaagt atttgtgaat ctagcttata
     6361 ttggcaaaaa cgaaaaacag atcgtcatca actgggaatt gattgattca cttatcactc
     6421 aattgacaga cggaattcaa caacgatacg gtagtcaaaa tcaagtgtca attagtcgga
     6481 tggcggaacg cttcgcgctg gacgaaaact tcgtaacggt agaagaaaaa ccggaagaga
     6541 gcatggaaga attggcacag ttagctctag atacattgaa aagcgcactt gattctattg
     6601 aggcgagtcg agaaaaagaa ggtcaagctt tagctttagt gttgaaaaag aatactgaag
     6661 aattcaaaga agtactaaaa aaactcgata gttttgtaga aatctatgaa aaggaatttc
     6721 aaataaggta tcaaaaaaag ctggaggaat ttcttggagc gaaagtcgat gagcaacggt
     6781 tgttgacgga gctagccatc ttgttggaac gcggagatat tcacgaggaa ctggatcgtc
     6841 tggcgatcca tgtacaaaaa ctagatgaac ttatcgaagc acaatgtcct gtaggtcggg
     6901 aattagattt cctgatccaa gaaatgaatc gggaaatcaa cacgatcggt tccaaatcaa
     6961 atgcaatcga aattaaaaat caagtcgtcc agtctaagac gatcttagaa aaaatcagag
     7021 aacaagtaca aaatattgaa tgacgaaaga ggtagagata atgaaatatt atgacgtaac
     7081 gttccacgaa ttaagtggaa aaactgtggt taaacgggat atcccttctg aaaaaaacgg
     7141 ctttgacgta tggaaagatg cctgtgcaga ctataatgag aacgaactgt ttattttgat
     7201 caacgacggt gcttacgtga cgatgaatcg gaaatttatt gttcgaattg acacagaaga
     7261 ggtagaagat ccgacagaaa aagcacgttc tcgaaaagat gagatcatgg gtgtagtgaa
     7321 tactttgtcc aatatgggct tttagcaagg atgctgaaca aagaaataca ataaataatc
     7381 agaattggat ttcttcgctt agcttctttg ctctaggaat acatttaaag gttgtgacaa
     7441 aattcgtgtc acaatctttt tttattttga ataagtgatg ttcaaaaacc attttctcgg
     7501 tatta
//
LOCUS       DACEXQ010000133         7406 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.133, whole genome shotgun sequence.
ACCESSION   DACEXQ010000133 DACEXQ010000000
VERSION     DACEXQ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.133"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            120..1001
                     /locus_tag="IXH04_001921"
     CDS             120..1001
                     /locus_tag="IXH04_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1852517.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            1014..2624
                     /locus_tag="IXH04_001922"
     CDS             1014..2624
                     /locus_tag="IXH04_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAQ1852518.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2701..3360)
                     /locus_tag="IXH04_001923"
     CDS             complement(2701..3360)
                     /locus_tag="IXH04_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ1852519.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3643..4308)
                     /locus_tag="IXH04_001924"
     CDS             complement(3643..4308)
                     /locus_tag="IXH04_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852520.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(4455..5060)
                     /gene="dhaL"
                     /locus_tag="IXH04_001925"
     CDS             complement(4455..5060)
                     /gene="dhaL"
                     /locus_tag="IXH04_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAQ1852521.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(5088..6071)
                     /gene="dhaK"
                     /locus_tag="IXH04_001926"
     CDS             complement(5088..6071)
                     /gene="dhaK"
                     /locus_tag="IXH04_001926"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAQ1852522.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(6073..6468)
                     /gene="dhaM"
                     /locus_tag="IXH04_001927"
     CDS             complement(6073..6468)
                     /gene="dhaM"
                     /locus_tag="IXH04_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAQ1852523.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            complement(6558..>7406)
                     /locus_tag="IXH04_001928"
     CDS             complement(6558..>7406)
                     /locus_tag="IXH04_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1852524.1"
                     /translation="IYNVYRFLKSGGSAISYYEQYKKNKRNCGRRPIVLPDGQQEYIQ
                     KKVAQGWTPDVLIGRAEFPIACSVRTLYRMFKDKTFDPHDLPMKGKRKPNGHKEKRGK
                     QAFRRSIQDRTTDYIQFNHEFGHLEGDTIVGGKHKSAVITLVERLSKVIITLKPAGRQ
                     AIDIENRLNQWFQSVPKHLFKSITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGL
                     NEHSNGLLRKDGLYKAMDFNKVSETFIQSVASKRNHIPRKSLHYRTPLEVFLSYVGKD
                     DLSSLI"
BASE COUNT         1966 a         1559 c         1373 g         2508 t
ORIGIN      
        1 taaaaagaat attatgtact aaaataataa aaaaagtttt ataaaaaggt ataaaacctt
       61 tttattaaac tatttaatct tgtttagtat atatttttag aaaaaggagt ggggttttta
      121 tggcagaaat cgtcaaagtc acacatttga aaaagagctt tggtcgtttc gaagctttaa
      181 aagatgtctc tttcaccgta cattcaggtg aagtcgttgg atttatcgga ccaaatggtt
      241 ctggaaaatc cacgacgatt cggacacttt tagggattat aaaaaaagac ggcggaacgg
      301 caacgatttt tgggcaagat gtatgggaag atagtttatc tatccatgaa cgtctttctt
      361 atgttccagg cgatatctct ttgtggggaa atttgactgg aggagcgatc atcgatctct
      421 tcatgaagct tcatggaaaa gggagtgctc aaaaaaaaga tgaactgatt caacgtttct
      481 ctcttgatcc taagaaaaaa gcaaaaaact attcaaaagg aaatcgccaa aaagtgggct
      541 tgattgctgc tttggctgtt gattcggatc tctatttatt tgatgagcct acttctggtt
      601 tagatccttt gatggaaacg attttccaag aagaagttga aaaattgaaa gaacagggca
      661 aatccatcct actttcttct catattctca gtgaagtcga gcgactagcg gataaagtcg
      721 tgatcatacg cgaaggaaca ctcgatgaag aaggaacact cgatgaattg cgtcatttga
      781 ctcgttcgac catcactata gaaacagaag cagagaatag tcacttagca caattgaacg
      841 gtgtccatga ctttgtgcaa aaaggtcacc tcgctcaatt tgctgctgat aacgaagctt
      901 tcgatgtgat tttagctgaa gcgtccaaat taggtgtgaa aaaatttgaa tctattccac
      961 cgactcttga ggatttattc atgagacatt atgaaggatg aggaggaagc gaaatgactg
     1021 ataaattttc acgctggtcc accctttttt ctcaaaacct tagccgtgac tggaaaaaga
     1081 tcctgatatg gctagtcggt gtcactgctt tttctggtgg ttttgtccct gcgtttgaag
     1141 aattgacttc cggacaagga ttagctggat tatacgaaac cttgcaaaac ccggcaatga
     1201 ttgcaatggt cggaccaact ccagcttcga atgctgctga gtatacttta ggagcgatgt
     1261 atgcaaatga aatgttgctt ttttgcagtt tgtttgctgc tgtgatctct gttttacacg
     1321 tggtgagcca aacgagaaaa aaagaagaac ttggtctgat tgaacttatt cgttcctttc
     1381 gagtcggacg tcaagcaaac tctttagctg tcatcgctga aagtattctg atcaatttgc
     1441 ttttagcaat atcgatcagc tgtacgatga tttttttcca agcggattcg atcactgcag
     1501 aaggtgcttg gctttttggg ctatctgtag gtagtatggg gattttgggg agtgccgttg
     1561 cactgtttgc tgcacaactg atgccaagtt cagccagttc gaatggtctg tctttagcgt
     1621 ttttaggtgg actttacctt ctacgagcaa taacagatgt agctgattcc tccctttcga
     1681 tgtggaatcc tttaggctgg tcttatttga cctatccatt tacaactaac aactggattc
     1741 ctttgcttta tttgtttttg ttcagtctaa tcttgataat cagttcattt gttttagagg
     1801 aacatcgaga tttgaacacc ggctatatcc ccgaacgtga aggaaaaccg tatgctaaac
     1861 gttctctttt atccttgcgt ggtttattat tctatctaaa taaagggatg acaatcagtt
     1921 ggcttatcac ttttggtttg atgggggcgg catacggctc tatctacggg aatatgcaac
     1981 agtttttaga aagtaacgac ttaatgaaac agatgtttat ccaatctggc gtctcaattg
     2041 aaaaaaactt tacaagcacg atcatggtcg tcttgatttg tctagctgct attttaccaa
     2101 ttgctagcat caatcgttta tttacagaag aaacccacgg acgtatgaat cagctgttcg
     2161 caacaaaaat ttctaggaac caactttatt ggaccaatat gttactagct attctatttg
     2221 ctatattggc aatcctgatt agttctggtg cactagggct ttctgctttg gcttcgatga
     2281 aggatgcgaa agacatggat ctattgatgt ttcttaaagc cgggttcaat ttcctgcctg
     2341 ccgttttagt gtttatcgga attagtggct ttctattcgg atggcttcct cgaatcggta
     2401 aacttgctta tatctactta gcttactcct ttgcactcaa ttactttggc ggaattttgg
     2461 atttaccaga atggttttcc aaaacagctg tccctagctg ggttccacgg atgccaaacg
     2521 attcttttga tctttccgtg ttccttacgt tatcatgtat cagccttgca ctgttcgtcc
     2581 ttggttctat tggttatagg cgaagagact tactagaagg ataataaaaa agcatgtaca
     2641 atttcatgct agcctccaaa tctgggtcaa aaatccaaca tttggaggtc atttttttat
     2701 ttacaagtct tctaactgct ctttatcaat gatccagact tctcgattct ttctttttac
     2761 ataaccattc tcctctaata atttaaattt tctagataaa gtttcggggg tcgtaccgat
     2821 aaatgcagca agttctttca ttttcattgg caggaaaaag tgatccttcc ctgtcgcttt
     2881 agctaagtca agcagataga cagcgactcg ttcttctact ttttccatca tcaaaaaagt
     2941 tgtctgctgc tgtacttttg ttaatttctc aacgttgatt gctaaaagtt tcagactaag
     3001 ggcgggataa tgcagtaaga gttccgagaa atctttttgt ctcaatatgc aaatttccgt
     3061 tttttctagt gcttcgccaa ataaagactc atttggaacg cctagcaact ggctctctcc
     3121 ctcatatccg cccggctcta ttactcgtaa aagctgttcc tttccgctag cagataattg
     3181 atacactttc atactgccgg aggccacgat cactaattga ggttctccat tcggcgcaag
     3241 aatctgttct cctttttcca catagtgatg tttgacaagt gcgtgtactt tttcttgatc
     3301 ttgctgtact aagtgattga acaaaggaac aaatgaaaca cataaatgat catgactcat
     3361 aatctctcct ccttctaaac agctatttct aataaagaaa cataacttcc cttattccaa
     3421 ctgataattt tcttttatca tagcataaac gtcaagcgaa ccagatacca gcaaatagag
     3481 tggatttctt tttgtccctt gctttatttt ccttcttttg agaccttaag taattgattc
     3541 ctatcaattt tttaaatatt tactttttga ataatcaaaa acaaaccttc actcgcaagc
     3601 cgagagtaag gtttgttttt cgcattgctt atttcgtgtt gtctattgaa atttttccgc
     3661 acctagcttt tgatatagtg gtgctagttc ttgtaaggtt tcagcggtta tttgctcaac
     3721 gtgttcgatt tcttgcggat agaagaatag tttacccatt tgaaaggttc ggtctctcat
     3781 cgcttttttc aactcttctt gtttctttgt atcggacaaa tagtcagaca aagctaagtg
     3841 gtcgtcaagt tcatgttctg gttgcggcca tatgtaataa tccttgatat cgactgtttt
     3901 gctccactct ttcagtatcg caagcaactg gttatacgct gcaatgctac catatcgttc
     3961 ttcgctttta taatgttggt acatcaaata gatttggtat tgtttttgat tcaacaaaac
     4021 tgctagacag gcattctcgt cttgacgaga ttcgcttcgg tacgcgctcc aaaaatgact
     4081 gcgtaaattc cagccattcg tccagctttc gattttaggt ttgctcatgc cgtacatatc
     4141 aggcaaaagt gcagctgtct ggagctgaat ctctttccat ttttgccagt gttctttata
     4201 ttctttcttg atttgttcaa tcttatctgt tggatggctc tctttgagct gtttgaatgc
     4261 gtagattggt cgatcaaaca actgataatc tgattttcca agttccattg tttcgcctct
     4321 ttccgttatc ttttgtattc tacctgaatt ttgcgatatg gacaaataga ggttgtgaaa
     4381 aagctgttct gcatcaagtc ataagaacag ccaaacaaaa aactgcgatg agcatcaccg
     4441 cagttttatt gaacttaagg ttgattgatc acatctaaaa gcgttgaaaa taaaatggca
     4501 ctagaagcag ctcctgggtc gatatgtccg attgcacgct ctcctagata ggaagctcgt
     4561 ccttttttag ctaccaagac tttggtgtgt tcttttgcct ggtcaatgat ttcttttgtt
     4621 aaattccctg ccttcaatgc ctctgtgact ggcagccaaa catctaacat cgttttatcg
     4681 tcagcttctg cctttcctcg gctttgaata cccgctgttc cctgctcgat cacttcgcct
     4741 aatttttctt gagaatcaat agaatcgacc tcttttgtcg ctttcatcat gttcatgaat
     4801 gcagatccgt ataacggacc agaagcccca ccgactttcg atatcatcgc catagagaat
     4861 actttgaatg tatcgctgat tgtttcagga tgatcggctt gaaaagcttc ttcataagct
     4921 gaaacccctc ttcccatgtt atttccatgg tctccatcgc caatcggggt atctagatca
     4981 ctgagatatt gtttgttctg attgattgcc tctgcaaatt gcgtgagcca ttcttggatt
     5041 tcttgaacgg ttagtttcat attatcaccc ttttatcttg agatttttta ccatgaaatc
     5101 gtctgtacat tgctttctaa agaatctttc cattgtgtct ccgtcaagtc gatcagtgtt
     5161 agagaaagac cttgcatatc tagtgaggtc ataaaattac cgacttttcg gaaaactaca
     5221 ttgacgcctc tttcttccaa cagagctaaa acatcattca tgaacacgaa ctgttccatc
     5281 aaaggcgtcc cgcccattcc gttgactagt acacctgctt cttttggtaa attcgagtaa
     5341 tcatctaaaa tcttagtaac aagttccttt gccagtactt tagaaggctg catcttttct
     5401 cgacgatagc ctggctcacc gtggatacca acaccatact cgatctcatc ttctcctagt
     5461 tcgaatcctg gtttccctac ttctggtaca gttgctgctc gaagcgctag accgatcgtc
     5521 ttagttgcct gtacgacctt ttctcccagt tctttcaact cagctaggga agcaccgttt
     5581 cttgctgcat cgcccacgat tttgtgtaca aggacagtac ctgcaactcc gcgttttcca
     5641 gcagtatacg tactattttc aacggcgata tcatcatcta cgatcacact ttctacttca
     5701 atatcgtcca tagcagctaa ttcctttgcc atatcgaagt tcaatgcatc ccctgtataa
     5761 ttcttaacga tcagcaaaac tccttttcca ctatcggctt ccttaatagc cgtctgaatc
     5821 tgatctggag ttggagaagt aaacacatca ccaagcaccg ctgcacttaa catcccttcc
     5881 cctacaaacc cggcatggga tggttcatgt ccgcttcctc caccagaaac caaaccaact
     5941 tgttccgctt tctgattctt ggctacaaca cgggaagatt ctacacgatg aacaagttct
     6001 ggatagcttt ttactaatcc ttccagcatt tcttcgacaa tattgcttgg atcattcatg
     6061 atttttttca atttattccc atcctttctt tccggcattt tgtgcttctg ttaagatttg
     6121 ctgagtatca tctgttatac cggcagtgat ggctgctgca aaagctcctt ccactaaagg
     6181 cgcatccacc agatgataac gttgctgctg ctcttcttcc aacatgtctt ttgccagttc
     6241 cgagttcaat accgcgctac cgatatccgc aaaaacaaaa taactagctg catcgcttga
     6301 ttcattgact gcttctacga ttttcatcgg gtctgatccg atctcgccat ttattccgcc
     6361 gagagaaaaa atttgcacct cttcggattt ttgcatctgc tcaatcattt ctttaatacc
     6421 atctgttact ttttgactat gagaaataag caaaatactt tttttcacaa gaatcttcct
     6481 ttcttttaca tagttgctaa cgattggttt cgctttcatt aatgaacaat tcaagtatat
     6541 aataaatttt tcgtttgtca aattaagcta gacaaatcat ctttacctac gtaactcaaa
     6601 aatacttcca atggtgtgcg atagtgtaat gattttctag gaatatgatt tcgttttgat
     6661 gccaccgatt ggataaaggt ttcggatacc ttattaaaat ccatagcttt atataagcca
     6721 tctttacgta ataagccatt cgaatgttca tttagtcctc gttgagaagg tgttcctgga
     6781 tcagcgaaat aaatcgaaat atcattggta ttactaatct ttttccaatt cgagaactct
     6841 tttccacaat caaatgtgat ggatttaaac aagtgtttcg gtacagattg aaaccattga
     6901 ttcagtctat tctcaatatc tatagcttgc ctaccagcgg gtttcaaggt gatgatcact
     6961 ttagataatc gctcaactaa cgtgatcacc gcacttttat gttttccacc gacaatggtg
     7021 tcaccttcta ggtgaccaaa ctcatgattg aactgtatat aatcagttgt tctatcctga
     7081 atcgaacggc gaaaagcttg ttttcctctt ttttctttat gtccgtttgg ttttcttttc
     7141 cctttcattg gtaaatcgtg agggtcaaag gttttatctt taaacatccg atatagtgta
     7201 cgtaccgaac aagcaatagg aaactctgct cgaccaataa ggacatctgg agtccaccct
     7261 tgagccacct ttttttgaat gtattcttgt tgcccatctg gtaacacaat cggacgtctt
     7321 ccgcagttac gtttgttttt cttatattgt tcatagtagc ttattgcgct tccaccagat
     7381 ttaagaaaac gatagacatt ataaat
//
LOCUS       DACEXQ010000134         7401 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.134, whole genome shotgun sequence.
ACCESSION   DACEXQ010000134 DACEXQ010000000
VERSION     DACEXQ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.134"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            68..151
                     /locus_tag="IXH04_001929"
     CDS             68..151
                     /locus_tag="IXH04_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAQ1852525.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            276..1013
                     /gene="erm(B)"
                     /locus_tag="IXH04_001930"
     CDS             276..1013
                     /gene="erm(B)"
                     /locus_tag="IXH04_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAQ1852526.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1183..1443
                     /locus_tag="IXH04_001931"
                     /pseudo
     CDS             1183..1443
                     /locus_tag="IXH04_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
     gene            1606..2271
                     /locus_tag="IXH04_001932"
                     /pseudo
     CDS             1606..2271
                     /locus_tag="IXH04_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            2307..2552
                     /locus_tag="IXH04_001933"
     CDS             2307..2552
                     /locus_tag="IXH04_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ1852527.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            2655..3524
                     /locus_tag="IXH04_001934"
     CDS             2655..3524
                     /locus_tag="IXH04_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAQ1852528.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3505..4239
                     /locus_tag="IXH04_001935"
     CDS             3505..4239
                     /locus_tag="IXH04_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1852529.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4272..5184
                     /gene="ant(6)"
                     /locus_tag="IXH04_001936"
                     /pseudo
     CDS             4272..5184
                     /gene="ant(6)"
                     /locus_tag="IXH04_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
     gene            5181..5723
                     /gene="sat4"
                     /locus_tag="IXH04_001937"
     CDS             5181..5723
                     /gene="sat4"
                     /locus_tag="IXH04_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAQ1852530.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            5816..6610
                     /locus_tag="IXH04_001938"
                     /note="aph(3')-IIIa"
     CDS             5816..6610
                     /locus_tag="IXH04_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAQ1852531.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(6718..6906)
                     /locus_tag="IXH04_001939"
                     /pseudo
     CDS             complement(6718..6906)
                     /locus_tag="IXH04_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6886..>7401
                     /locus_tag="IXH04_001940"
     CDS             6886..>7401
                     /locus_tag="IXH04_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1852532.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIE"
BASE COUNT         2593 a         1119 c         1556 g         2133 t
ORIGIN      
        1 tgtgtataat aggaattgaa gttaaattag atgctaaaaa tttgtaatta agaaggaggg
       61 attcgtcatg ttggtattcc aaatgcgtaa tgtagataaa acatctactg ttttgaaaca
      121 gactaaaaac agtgattacg cagataaata aatacgttag attaattcct accagtgact
      181 aatcttatga ctttttaaac agataactaa aattacaaac aaatcgttta acttctgtat
      241 ttatttacag atgtaataac ttcaggagtg attacatgaa caaaaatata aaatattctc
      301 aaaacttttt aacgagtgaa aaagtactca accaaataat aaaacaattg aatttaaaag
      361 aaaccgatac cgtttacgaa attggaacag gtaaagggca tttaacgacg aaactggcta
      421 aaataagtaa acaggtaacg tctattgaat tagacagtca tctattcaac ttatcgtcag
      481 aaaaattaaa actgaacatt cgtgtcactt taattcacca agatattcta cagtttcaat
      541 tccctaacaa acagaggtat aaaattgttg ggagtattcc ttaccattta agcacacaaa
      601 ttattaaaaa agtggttttt gaaagccatg cgtctgacat ctatctgatt gttgaagaag
      661 gattctacaa gcgtaccttg gatattcacc gaacactagg gttgctcttg cacactcaag
      721 tctcgattca gcaattgctt aagctgccag cggaatgctt tcatcctaaa ccaaaagtaa
      781 acagtgtctt aataaaactt acccgccata ccacagatgt tccagataaa tattggaagc
      841 tatatacgta ctttgtttca aaatgggtca atcgagaata tcgtcaactg tttactaaaa
      901 atcagtttca tcaagcaatg aaacacgcca aagtaaacaa tttaagtacc gttacttatg
      961 agcaagtatt gtctattttt aatagttatc tattatttaa cgggaggaaa taattctatg
     1021 agtcgctttt ttaaatttgg aaagttacac gttactaaag ggaatggaga taaattatta
     1081 gatatactac tgacagcttc caagaagcta aagaggtccc tagcgcctac ggggaatttg
     1141 tatcgataag gggtacaaat tcccactaag cgctcgggac cccttgtagg aaaatgtcct
     1201 aagtgtggca acaatattgt attaaaaaaa tcgttttatg gttgttcaaa ttatcctgaa
     1261 tgtaagttta ctttagctga acattttaga aagaaaaaac taaccaaaac gaatgtaaaa
     1321 gaattactgg agggaaaaga aaccctggta aaaggaatca aaaacaaaga gaaaaagccc
     1381 tacaatgccg ttgtaaaaat tggggaaaag ggatatattg attttatctc tttttcaaaa
     1441 taaacatggg gctgacccaa aagtagctcc tatatgaaaa atagaggtct ttccatttag
     1501 atagtagtgg aaagacctct atttatcttt tgaaagtgaa tacaaaaata aagcacactc
     1561 tgctataata taggtgatta atccatttga gagaggtgct ttatcatgta tcaaaattat
     1621 accataaacc aactttgttt gcctatagat cttgaaatta aattagaaga aaatgatttt
     1681 gctcatgcca ttgtgcagtt tgttgactct attcccgatg aagtctttct tccttattat
     1741 caatctatgg gtagacccca atatcatcct cgtatgctac tttccattat tctttgtgcc
     1801 tatattcaag gggtttattc gggccgccaa atacaaaata tgctgataga tagtattcgg
     1861 atgcgctact tatctcaaga gcaattccct aactttcgta ctattaatcg ttttcgcgtt
     1921 catccgatta tgaacgatat tctagatcat tcatttgttc aatttcgtga gctactggtt
     1981 caaagtggct taatcagtgg cagtgctcta tacattgatg gtacaaaaat tgaagcggat
     2041 gcgaataaat actctttcgt ttggaaaaaa agtattttaa agtataagga tagccttgat
     2101 caaaaagccc ttgaaaacta ccaacaaatg gtggaaacaa aaatcttacc tgagttaata
     2161 gatgatttaa aggatgaact ttctatagaa gagattgaaa ggattcgtca atctttggaa
     2221 gaaaaggaaa atcaattaat tgaagcgatt gaccaaaccg aaacagtaga agggggctga
     2281 cccattatta ataaggagtg gacaacatga ttgatgtaaa tgagctattg gaagaagcta
     2341 ttagagaaac tgaaaatcta aatgatggcg aagtttttct tgttaaagat ttattcaagg
     2401 gatatgtggg gaataggata cccagaaagg atcgacttct gcttggaacg ttatttttaa
     2461 accatgttag caaaatgaat ggtaatttaa aggctattga aaagacttca tctaatcagc
     2521 agaggtataa aaagacaatt gataagtttt aactatcttt tgtgaaatta aaaaaattat
     2581 aaatatgttg agtagaagca aaagatttgg cttggaattt ttacagcagt gaatcgataa
     2641 aaggagcatg cttgatggta gataatatta ttaaatcagt agcagagaaa ttatcctctc
     2701 tgtcttatat agaaggtatt gttttaggtg gttcacgtgc aaggggcacc catacagagg
     2761 attcggatat agatatcggc atctattaca attcagaatc atttgacata aatactatta
     2821 atcaattcgc tacaaagctg gatgatgagc atagaaataa ccttgttgta cctcccggag
     2881 catggggtga ttggattaat ggcggcggat ggttagtcat aaacgggtat catgtggatt
     2941 taattttacg tgatattaaa cgtgtggaac aaataatgaa agatacagag cacggaattg
     3001 ttactgccaa ttatcagact gggcatcccc atggttatat tagtgcaatg tatcgaggag
     3061 aattagcgat tagcaaaata ctatatgcta agaatgaaag cttatgcgaa ttaaaaaaac
     3121 aggcagaaac ttatcccaat gctttgcaga aaagtttagt taactttttt atgtttgaag
     3181 cagggttctc tttaatgttt gtaaaagcaa attcgggaac agacgataaa tattatattg
     3241 cgggtcatgt ttttcgtata gtttcatgtt taaatcaagt gttatttgca tgtaataatg
     3301 cttattgtat caacgaaaag aaagctataa aactgcttga aacttttgaa cataaacctg
     3361 aaaaatatac cgagaaggta aatcatattt ttgaagtact cggtatctca ctttttgaat
     3421 gctacgacat gaccgagaag ctttataatg aagtgaatga aattgtatcg gagataaata
     3481 actttttaaa cgaggagagt tcagatgaaa gaaaacaaat atgatgataa tatatttttt
     3541 caaaaataca gtcaaatgag tcgctcgcag aaaggactgg ctggtgcggg agaatgggag
     3601 actttgaaaa agatgctacc tgattttaag ggtaagcgtg tgcttgattt aggatgcggc
     3661 tatggatggc actgtatata tgcgatggaa aacggtgctt cctctgtagt aggtgttgat
     3721 atttctcata aaatgctcga agtagcaaaa ggaaaaaccc attttccaca gattgaatat
     3781 gaatgctgtg ccatagaaga tgtggatttc ccagaggaga gctttgatgt aatactaagt
     3841 tcgcttgcgt ttcattatgt agcagactat gagaatttaa taaaaaagat atataggatg
     3901 ctgaaggctg gtggcaattt agtttttaca gttgaacatc ctgtttttac tgctcatgga
     3961 acacaagact ggtattataa cgaaaaagga gaaatactgc atttcccggt ggacaattat
     4021 tattatgagg gcaaacggac agctatgttt ttggaagaaa aggttacaaa atatcataga
     4081 acactgacca catatctaaa tacactgctt tcaaatagtt ttataataaa tcagattgtg
     4141 gagccacagc cgccagagaa catgatggat attccgggga tggcggatga aatgcgacgc
     4201 ccaatgatgc tgattgtatc ggcaaaaaag aagatgtaat aatatagaaa aaataaacga
     4261 ggagtatgta aatgagatca gaaaaagaaa tgatggattt agtactttct ttagcagaac
     4321 aggatgaacg aacgtattcg aattgtgacc cttgaggggt cacgcgcaaa tattaatata
     4381 cctaaagatg aatttcagga ttatgatatt acatattttg taagtgatat agaaccgttt
     4441 atatctaatg atgactggct taatcaattt gggaatataa taatgatgca aaagccggag
     4501 gatatggaat tattcccacc tgaagaaaag ggattttcct atcttatgct atttgatgat
     4561 tacaataaaa ttgatcttac cttattgccc ttggaagagt tagataatta cctaaagggc
     4621 gataaattaa taaaggttct aattgataaa gattgtagaa ttaaaaggga catagttccg
     4681 actgatatag attatcatgt aagaaagcca agcgcaaggg agtatgatga ttgctgcaat
     4741 gaattttgga atgtaacacc ttatgttatt aaaggattgt gccgtaagga aattttattt
     4801 gctattgatc attttaatca gattgttcgc catgagctgc tgagaatgat atcatggaag
     4861 gtcggcatcg aaacaggctt taaattaagt gtaggcaaga actataagtt tattgaaagg
     4921 tatatatccg aggatttgtg ggagaaactt ttgtccacct accggatgga ttcctatgaa
     4981 aacatatggg aagcattatt tctatgccat caattgttca gggcggtatc cggtgaggtg
     5041 gcggaaaggc ttcattatgc ctatccggag tatgatagga atataacaaa atataccagg
     5101 gacatgtata aaaaatacac tggtaaaacc ggctgcctgg atagcacata tgccgctgat
     5161 atagaagaga ggcgggaaca gtgattacag aaatgaaagc agggcacctg aaagatatcg
     5221 ataaacccag cgaaccattt gaggtgatag gtaagattat accgaggtat gaaaacgaga
     5281 attggacctt tacagaatta ctctatgaag cgccatattt aaaaagctac caagacgaag
     5341 aggatgaaga ggatgaggag gcagattgcc ttgaatatat tgacaatact gataagataa
     5401 tatatcttta ctaccaagac gataaatgcg tcggaaaagt taaactgcga aaaaattgga
     5461 accggtacgc ttatatagaa gatatcgccg tatgtaagga tttcaggggg caaggcatag
     5521 gcagcgcgct tatcaatata tctatagaat gggcaaagca taaaaacttg catggactaa
     5581 tgcttgaaac ccaggacaat aaccttatag cttgtaaatt ctatcataat tgtggtttca
     5641 aaatcggctc cgtcgatact atgttatacg ccaactttga aaacaacttt gaaaaagctg
     5701 ttttctggta tttaaggttt tagaatgcaa ggaacagtga attggagttc gtcttgttat
     5761 aattagcttc ttggggtatc tttaaatact gtagaaaaga ggaaggaaat aataaatggc
     5821 taaaatgaga atatcaccgg aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga
     5881 tacggaagga atgtctcctg ctaaggtata taagctggtg ggagaaaatg aaaacctata
     5941 tttaaaaatg acggacagcc ggtataaagg gaccacctat gatgtggaac gggaaaagga
     6001 catgatgcta tggctggaag gaaagctgcc tgttccaaag gtcctgcact ttgaacggca
     6061 tgatggctgg agcaatctgc tcatgagtga ggccgatggc gtcctttgct cggaagagta
     6121 tgaagatgaa caaagccctg aaaagattat cgagctgtat gcggagtgca tcaggctctt
     6181 tcactccatc gacatatcgg attgtcccta tacgaatagc ttagacagcc gcttagccga
     6241 attggattac ttactgaata acgatctggc cgatgtggat tgcgaaaact gggaagaaga
     6301 cactccattt aaagatccgc gcgagctgta tgatttttta aagacggaaa agcccgaaga
     6361 ggaacttgtc ttttcccacg gcgacctggg agacagcaac atctttgtga aagatggcaa
     6421 agtaagtggc tttattgatc ttgggagaag cggcagggcg gacaagtggt atgacattgc
     6481 cttctgcgtc cggtcgatca gggaggatat cggggaagaa cagtatgtcg agctattttt
     6541 tgacttactg gggatcaagc ctgattggga gaaaataaaa tattatattt tactggatga
     6601 attgttttag tacctagatt tagatgtcta aaaagcttta actacaagct ttttagacat
     6661 ctaatctttt ctgaagtaca tccgcaactg tccatactct gatgttttat atcttttcta
     6721 aaagttcgct agatagagtt ctatattaga gatgtaaaga gttttggtga agaggtttgt
     6781 gagtatggct tttataacca gggtgcaatg agaagcacta acagcagcta gcttaagaac
     6841 gcataggata tactttttat ggaagtcaca gaggaggaat aaaatttgaa caggattaac
     6901 agagtaacct ctattcttat tcagctgcaa tcaaaaaaaa taattcctgc caaagaaatt
     6961 gcacagcgat ttaatataag cttaaggaca gtttacagag atatccggac acttgaagaa
     7021 gccggaatac caattggatc tgaggccgga aaaggatatt ttcttgtaga gggcttccta
     7081 cttccgccgg taatgtttac agcggcggaa gtgggtgcat tgattacagc agggaaattt
     7141 ttaaattgcc atggagatga atcatttata aaggattttg attcagctat gtataaaatc
     7201 aaatctattt tgaagcatgg tgaaaaaaac tatgcacagg agttggagaa cagtattaac
     7261 gtgtacagca catctggaca gaaaaataca ttggcggata acgttattgc agcaattcag
     7321 actgcaatct gcaataaaag ggtaatatca attcaatatc ctgcatcagg agggcaagaa
     7381 ccggaaagca gaatgataga g
//
LOCUS       DACEXQ010000135         7285 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.135, whole genome shotgun sequence.
ACCESSION   DACEXQ010000135 DACEXQ010000000
VERSION     DACEXQ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.135"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(260..1138)
                     /locus_tag="IXH04_001941"
     CDS             complement(260..1138)
                     /locus_tag="IXH04_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1852533.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(1177..2295)
                     /locus_tag="IXH04_001942"
     CDS             complement(1177..2295)
                     /locus_tag="IXH04_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAQ1852534.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(2292..4391)
                     /locus_tag="IXH04_001943"
     CDS             complement(2292..4391)
                     /locus_tag="IXH04_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAQ1852535.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(4442..5263)
                     /locus_tag="IXH04_001944"
     CDS             complement(4442..5263)
                     /locus_tag="IXH04_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1852536.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(5260..6069)
                     /locus_tag="IXH04_001945"
     CDS             complement(5260..6069)
                     /locus_tag="IXH04_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1852537.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(6090..6584)
                     /locus_tag="IXH04_001946"
     CDS             complement(6090..6584)
                     /locus_tag="IXH04_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1852538.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(6596..7084)
                     /locus_tag="IXH04_001947"
     CDS             complement(6596..7084)
                     /locus_tag="IXH04_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1852539.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
BASE COUNT         2207 a         1681 c         1218 g         2179 t
ORIGIN      
        1 gaaaaaatta actagcacca cgggtaatat attactattt aagtgtacgc caccagtgaa
       61 tatcctcaag gtatacagcg cacaacaaaa agaaaaaagg aaacgcagaa gcatttcctt
      121 tcagtgattt aatgaaaaca acacgatttg acttagagcc tttttatttg aattcatttt
      181 cgctttatcc aacttcattt gatcatttga ttactcggtt aaatactttt gacatgagta
      241 tcaatgaaac attcattaat tatggttgct tatttcttac cgcttttctg gccaatgcta
      301 atccgccgat tattccggca ttatcaccta attcgggcgt aataatgtag ctatcaattg
      361 gtggtatttc aacatatcca tttataagct caacaaattt atcctgaact tttttcttca
      421 tgtgttcttg tttcattaca ccaccgccaa aaataattct atctggcgaa aacatcagtg
      481 ttgtattgta tgcacattgg gcaaggtaaa aggcttcaat ctgccaaaac gaatcgtcag
      541 ccaataaatt ctggcctttt acacccaacc gtttttcaat cgctggtcct gctgccatcc
      601 cctctaaaca atcttggtgg aagggacagt ttcctgaaaa attgtccttt ggatgtcgct
      661 tgatcaacat atgccccatt tccggatgac tgaacccttc cacgaaagtc ccattgaaca
      721 aggcaccacc tccgatacct gttcccacag tgaagtatgc aacacctgct aaccctttgc
      781 cagaaccagc aacatattca ccatagcaag ccgcattgac atctgttgtc cattcaatcg
      841 gaatattgaa atattgcttt agcgttccta caaaattgaa attttgccaa gcaagttttg
      901 gtgttgatgt gatatgaccg aaactcgttg atttgcattg aatatcaatt ggaccaaagc
      961 agccgacccc gatgcctgcc aattgttcct taaaaggttt aaaaaaatca atgacctgct
     1021 tcattgtttc aactggagtt gttgtgggaa aactgactct ttccaaaatc gttaattcct
     1081 cagtaccgac tgcacagaca aatttagtcc ctccagcttc aattgcccca tatatcatca
     1141 taggttcctc ccttcgaata tgtgaaaaac acttcattaa cgcggcaagc gttctcgatc
     1201 agtgaaatga cgtttagtat ttcctgaagc ttctgttgaa taccagtaag cgacactagc
     1261 aacatcgtct tgccgctcaa ataagcgaac atcatcgttg ccgatttgtt gtaaagtgac
     1321 cgataaatct tcggtaaaac agatggggtc ttgaagatgc caacgataaa gaccatgtcg
     1381 tggcattgcg tcgtcaccaa aggcatgaac ggcattcaat tttcccgttt caaaacgatc
     1441 tcttgtggca tctctatttg attggtaagg ataccctaaa aaggctgttg aatagttttt
     1501 cgcatgtgga cgtccgaact catcgtactc atgaaaggcc cacgccccgc caaaataatc
     1561 ttctgaacct gttgaggtta cggtcggata ctcactatcc ccatcaatgt agaatttaaa
     1621 ctcgccttcg ccccaccaat aacgttctag gcaggtaagc gcaaggtaag tacccacgta
     1681 atagccttga ccctttaatc gatcaattaa cttataatcc tcctgtaatt tggtaatgcg
     1741 ttcccgatgc caatttgcgt gaaaatatag gctattttct tccaattcgt ccaccaaagc
     1801 gtaatttatc gtataaaaaa atgaggacat atccttcgga tgtaaattct cgatcgtaat
     1861 tttagcggat gtttggaaag gcatttcgaa ataggaatta aagccccctg taggattaac
     1921 cactatcggc aacgagttca cctcgcaacg ctctccaaac ccattacaaa agaaatcccc
     1981 taaaggactt tcaacagcag ggctatccga cccatcccaa taaattctta agattaaatc
     2041 acgtaaaaca aaactgccaa attctgttcg atcacgaatc gtcatccaca tatgacgaat
     2101 tattcccgtt ccagtaatat ccgcaatcac agtttcttca ccagaagtca gcggaattgc
     2161 gccacgacct ttgcggcttg gtcctagctc gcttggcaac atcgcagctt tacctttctc
     2221 gcctgttgga ttttcgggag tgattgctcg actctggatt tttttaaatt gggtaatatt
     2281 ttttagtata ctcatctttt tctcctttga atgtgaatcg tttttccctt ttctacgtga
     2341 agaatttttt tcgtatcact tattttcaat ggcttttctg acaaatttga aatactcaac
     2401 tcggattgcg tacattcaat cgaaatgaag gcttgaccga acttcaaatt cttccaagaa
     2461 aacgttaaat tatcaacagg atcaatttgc aatactcctt ttttgaattt caatccgaaa
     2521 tattgactga tcattaggct gacaaaaaga cccgatgccc agccgcattt gccgcagcca
     2581 aagtctcgca cgacttctcc ttgttgagcc tctaagcgat acggccacca ccaccacgaa
     2641 ccgtctaaat ctgccaaaca aattagttct tctaatcggg ttacgaaagc ttgagggttg
     2701 tgcagctcgc tcattaaaac agtgataaag ccaggaaaag tggcgccgga ttctaatccc
     2761 caagtgatgc ctttgatttc ctcgctatag gttggattga aagaactagc agaaaaggcc
     2821 atggtgttct gatataaatg atctttatta tctaaaaatt gataaaaagg catcaacgta
     2881 gtgtctgatt cttctccatc atgcatcaat aaattaattt ttccatccac aatcacatca
     2941 cttaaaaaga ttaacccttg ctcaagaatc ggtttttgat agtgcttcac tgaataggtt
     3001 gttttctcct catcaccgat tccctccaaa aattgctcac caaaaggacc atcgatagtc
     3061 atttgtttac aaattgattc ctttagttta caggcaatgt ttttgtatcg cttgctaaga
     3121 ctcagttgtt ctagggcttc aaaaatttct gcaagtccaa tacaggcctt ccaaagacaa
     3181 agattgctgc cggtatgata ctttccaagt gcaaaggcgt ctgataacca aacagatgga
     3241 aaaagcattg gttcccctga ccgacgttga gctaatatgt cctcaataat tcttgaagca
     3301 ttcagtagaa cctgcggaca ccgctcaaag aattttgtat ccccagtatg ttcaaaataa
     3361 agactagcca gtaacgcact ggataacgaa ttactcagag aatgattgat acctcctgta
     3421 aattttgaac caggaaactt aacgccatat tgatcaaacc atagaaccgt tttttgacat
     3481 aactccggtt caaacatggt gaatggcaat gatgcgtaat acatatcctt attccaaatt
     3541 cgattagtag caggatagct gccccaattg ctgccgacga cctgtccctg tcgattcata
     3601 ccgaaactag aaaagctttg ataaagtgca cgacgataga gatggacaac tctatcgttt
     3661 ggcattgaca gctgcccaaa tagctgagca aaatagttca atgtttcctt cagccatttt
     3721 tcgatatcag attcctgaaa agtcattact tcttcaaaag catttgggtc cacaaaagca
     3781 atggcgaagc ttgccacttc ctgttgtgcg atcgtgtcct tatttccttt ttgagaaatc
     3841 aacacatttt gctgatccga atacttctct tgatacgaag gcacatcttt cactgaaact
     3901 tgaaggcttt cgtgtgtttc attcacaatg taaacctgtt gaacaagcat acttaaacgc
     3961 ttattagcca gtatgggagc aaaggatact acaatgacct taaacgtcga ataatgatga
     4021 atatatctag gcaaacagtc ttgaatcaca tcgacttcaa tacgatgatc cgattctgac
     4081 aaatggtaga caatcccgtt tacttcaacc tttaaggata acgaattggt ttgagagagc
     4141 tgtttattga cccaaacacc tggtttttcc tcagttagta aattttctcg ataaaaggtc
     4201 aacgtttgaa tcgtcccgca aacatcaagg tttgctaatg tcttttgatt acttacatcg
     4261 aaggtcaatc cctttacatc aggaatattc cgatagtagc tgtattcgtt tgaagtagtc
     4321 acatttccgt cgtttaactc agcattaaaa aatgggtgtt tgggtgaata aattacttca
     4381 ttaatagtca tcttttccct ccttgctaaa aaaactgcag cctagtagct gcagcttcct
     4441 actatgcgat tccaagtaat gacaataaaa ttcccaagac gaccattcca aaaataattt
     4501 tgatgacgcc gactttttta tctagtaatt tgaaacagct caatgttacc aataacggta
     4561 caattccttt aaaaatacta tcgaggattt cttggacatt cattagttct tcgcccttca
     4621 ttgaaaagct caaaacggta ttgaatttga ccatactgat cgtcattgca cctaccatga
     4681 tcagacccaa gatactagca gcttttgtca gaatttcaat taacccgttc gcatacagct
     4741 tttcaatata tttagagcct aatgaatagc ctaaaacgcc gccatagtat cgcatcaagt
     4801 aattaggtat attgaacata actaaaaaga taattggtgc caaaatattt ccggcattgg
     4861 ctaagttgat cgctagtccc gcggtaacga cacgccaaac tccccagaac aatgaatcgc
     4921 caattccagc caatggtccc atcaaactcg ttttgatcgc gttgatactt tccacgtcaa
     4981 aaccagcttt ttcagaattt tccttctcca tcgatgccgc aatccccatg acaaaggacg
     5041 cgcaaggaat ggtcgtcgaa aaatactgtg tatgacgttc cagcgccgca gctcgatctt
     5101 cttcactttt gtagaaatga ttaatgaagg gcatcataga ataggcgaaa ccaccgccgc
     5161 ccatcgtcac cggattcacc gaagcataca tagtcatcga gcgccaaaac atcttccgaa
     5221 ccaatttttt ctcatcttta gaaattccat cagctaattt cattgaaaga aatcctcctc
     5281 ttcttggtca agcgttgcat ttacagcagc atcgctttga actgcatgaa cagatatatt
     5341 ttttagttgt agatctcttt gggctataac aattgcaata atcgttccaa ctgcggcaat
     5401 tgcaatcaat ggtaactcga gatacgccat aacaacggcc ccaaagaaaa accacacagc
     5461 aatattttta ctccaaagca tccgtaacag catcgccatc ccaactgccg gcagcagatt
     5521 tccagcgact gttaatccgt tcatgatgac ctctggaata ttatccaaga cgcttttgac
     5581 aacatctgct ccaaataaaa cggcaaagaa tggaataaga gcataaagcg catagtggac
     5641 aaaccacata aagaagtgca gattgatgat tcctttttga tcaccagatt ttgcaaattt
     5701 gaaaaattta gcagccatcg gtccacatac aaaagaaaaa atcatattca ttaactgtaa
     5761 tcctaatact gctagtggaa agacaatggg aataatcgca tccacgtttc ctcccatttt
     5821 gattgcgaac gctgtagcca gcccagcagc caaagaaggc tccgccgtac tagagattcc
     5881 gatattgacg acacccataa agatggcttc tagtgacgcc ccgacaatca accccgtctg
     5941 caaatccccc atcaacgctc ctgctaacgg agcaacaaca atcggacgac tcagcatgga
     6001 catcccgaac atttcatgtc cagcaactcc aataaaaacg gccaaagcga ctacaaatgc
     6061 atcatacata atttttctcc ttcctttttc taaaaatcac tcaaaataga gatagctgaa
     6121 cgtttttcca tcgccggtgt aggctgaaaa acggtatctg gataaagttc aactaattct
     6181 tttaagtctt gaatttctga cgtcgttaac atcacttcct ttgataaggt tttcttatct
     6241 tccgatggcg gtgtcatttt cccagcattc cccacgttca ttaattcgat ttccgttggg
     6301 aaagctttcg caacttttac cgcatctttg acattaggga ctaaaaccat caagcgcatg
     6361 tccttcgccc gcggatcttt taacaaatga atcgcgtcat caactgtttt aataatgact
     6421 ttgacattcg atggtgcagc catcttcaat gtcatctttt gaatatcgtt ggtagccgcc
     6481 tcatcgcttg ctactactaa cccggttaaa tttagactct ttgtccaagt catcgctact
     6541 tgtccatgaa ttaatcgttc atcaattcgc actgctttaa tcatttttct tcctcctaaa
     6601 aaaaatcttc ctcttcattt actacatgta cctcatcaag aatggtcatc tttaattcct
     6661 gttggcattt atttaccagt tcctgaatat cctcctttgt taattccttg gttgccaata
     6721 ctatttcgag cagaattggc agattaaagc cagacaaaat agttacagca atctctttct
     6781 ccaaaaaggt tttggtaatc ttctgattga cacttccgcc aaataaatcg gtaaaaacaa
     6841 aaatctgatc ctgctcgttc acctggttta caaatacatt caactcctgt tcaaaatcac
     6901 actcatccac ataagcatta atcaccgtaa tctcttgaac cttccccgtc agtaattgaa
     6961 tactgctaag aaagcctgaa gctaagtgac cgtgagtcgc tattagaatt ttcttcatat
     7021 ggttaattcc tttctttctg ttcactttta tataagcaat tcctgtgcca acttttaaaa
     7081 acattggttt aatcacgtta attcacatta aaaaaagtgt ctaacctaaa aaaaacagat
     7141 tagacacttt tttagacact ttatttttga atatacggtc ctaataattc atagagcagc
     7201 gaaagctcat aagcattgac tttaaaatta tatttcatct ccatagattg aaaaatactt
     7261 ctagaaacat aataaaattc atctt
//
LOCUS       DACEXQ010000136         7229 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.136, whole genome shotgun sequence.
ACCESSION   DACEXQ010000136 DACEXQ010000000
VERSION     DACEXQ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7229
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.136"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..309)
                     /locus_tag="IXH04_001948"
     CDS             complement(<1..309)
                     /locus_tag="IXH04_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HAQ1852540.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     H"
     gene            437..790
                     /locus_tag="IXH04_001949"
     CDS             437..790
                     /locus_tag="IXH04_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ1852541.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            802..1968
                     /locus_tag="IXH04_001950"
     CDS             802..1968
                     /locus_tag="IXH04_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1852542.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(2060..2680)
                     /locus_tag="IXH04_001951"
     CDS             complement(2060..2680)
                     /locus_tag="IXH04_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAQ1852543.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(2872..3168)
                     /locus_tag="IXH04_001952"
     CDS             complement(2872..3168)
                     /locus_tag="IXH04_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ1852544.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            3372..4022
                     /locus_tag="IXH04_001953"
     CDS             3372..4022
                     /locus_tag="IXH04_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1852545.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4038..5765
                     /locus_tag="IXH04_001954"
     CDS             4038..5765
                     /locus_tag="IXH04_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1852546.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            5762..>7229
                     /locus_tag="IXH04_001955"
     CDS             5762..>7229
                     /locus_tag="IXH04_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1852547.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDA"
BASE COUNT         2264 a         1290 c         1493 g         2182 t
ORIGIN      
        1 atgatagttc gccccgtata gtaattgacg tgcttcatca accgctacat aacacaaagg
       61 agcgccagac tctgttaccg cattcaaaaa atgaaaatcc ttatcgtacg caccggcacc
      121 acctaatcca tccacactag taactgtgta aagattgcca gctttacttt ttgctacata
      181 ggtagggcta ttttcttcaa cagctaatga aaggttggct aattcttctt tttctgtatc
      241 aaggtcgatc gtatagattc ctttactttc gcgacgcgta taggttccta aaattatctt
      301 ttcgatcata tgagctgatt cctcctaatt gtaatgaaat agttttcgtt tttattctat
      361 cacataatta ttccacacgg ctaaagaatg agaatgtgtt atactgtttt tgaaagcaat
      421 taaaggagag agtgcaatga ttcaggcaaa gattacagaa attgggacag aagcaataaa
      481 caaggaagaa cccatgctga ttttgttcga taaaacagca acagctgcct tacgaaaata
      541 ttcggtgatt caagaagtat caaaagacga acctttttct ttgaaagcag gcggaacaat
      601 tgcttttgat gatcaggtat atacgatcga ttatgtcggt ccgatggcca atgggaattt
      661 aaagacagta gctcacgtat cattgatttt tgatgcttgt ccagaagaag gacaaattgc
      721 aaatggaatc tatcttcatc cgcatagact gcctgctatt ggtttgggaa gccaaatttt
      781 ttataactaa caggaggaat tatggaagga aaaaataaga agaacagacc ggtatcgtgg
      841 ttttggcgct ggtttttaaa taatcaagta gtcacagcct tacttgttgt attgttaata
      901 ttgctgatcg tctttttatt tacaaaagtt tcatatttgt ttgcaccaat ctggcaattt
      961 ttagcaatcg taggtctgcc tatcatttta gcaggaatct tgtattactt gatgaatccg
     1021 gtagttgatt actttgaaaa gcgaaaagta ccaaggcttt acagtatcat tgggctgttt
     1081 gttttggttg ttgcactaat cgcatggggg agcgtagtga tcatccctaa gattcaggaa
     1141 caaacagtga gcttcatcag taacttccct caatatgtag atacgattga taataaatta
     1201 accgagattt tgcgggaccc gctgttcaac caatttcgcg aacagttgga aacagcaggt
     1261 gataagttca tgaattcggc aggcgatatg atccaagata tatctaagag tacagttcag
     1321 agtcttggaa gttttgtcgg cgcggtagcg actattttag tggctctgct gacgatgccg
     1381 tttatcttat tttatctatt gaaagacggt aaacaattag caccttattt tgtcaaattt
     1441 ttaccgacac gcatgcaaaa accgacgttg aatgtgctga gtgaaatgaa tagtcaagtt
     1501 tcttcttata taagaggaca actaactgtg gcttttgctg tggcaatcat gtttatgatt
     1561 ggttttgcgg tcattggttt agagtatgct gtcacgttgg ggattattgc gggtttttta
     1621 aacctgatcc cttacttagg atcattttta gcgatgattc cggctatctt tttagccatt
     1681 gtagcaggtc cattcatgat catcaaagta ctgatcgttt ttgcagtaga gcagacaata
     1741 gaaggacgct tgatctctcc attagtacta gggaatcagt tagcaatcca tccagtaacg
     1801 attttattag ttctgctgac ttctgggaaa ttgttcggaa tcatcggtgt gatcttgggg
     1861 attcctgttt atgcagcagc taaagtagtg attactcatg tttttgaatg gtacacaaca
     1921 atctcttctc tttatgaaga aaatccgttg aaaaagcaga aagaataaca aaaagagaca
     1981 gatgggaaaa tatccaaggc acaaatgctg ctttgaagat tttttcctct gtctcttttg
     2041 tatgcattta tctcaatttt taaaatgatt tacctaaagc agcagcttta tctaatgcat
     2101 tttgcatcaa ctcatctgca cggtcagggt cataatcgat tccttcgatg aatagctgat
     2161 ctacttgatc gatgcctaca aagtttaaaa taccttttac atattgagag gcaaagtctt
     2221 gtccgttgta tacaccgcca ttagattgga tgtgcagtgc ttttttacct gtcactaatc
     2281 cttctggccc gttttctgta tagcggaacg tttttccagc tacattgacg gtatcgaacc
     2341 aagcttttaa ttttgttggg atatttagat tccataaggc gtttgcgata acgattttat
     2401 cagcagcaag gaactgatca gtcaattcat tgaaacgagc aattttttgt tgttgacttt
     2461 ctgataagga agtgaattct gcaccagcac gtaatgcttc ccaacctgat aataattctt
     2521 catcgatttc cggaatatca tcgcgataaa gttccaaaac agtcacttca tcatctggat
     2581 gattttcttt ataacttgtt aaaaaagacg tcaatgcctt aactgtgcgc gattcttctg
     2641 cagttagggg atgacctttg actactaaaa cagatgccat atgcttacct tctcctttta
     2701 ttgctatgat tgttataact atgactaaca aaatcagttt actcgaaaaa gaaaaggcta
     2761 tcaagtcata ttgataaggt tgaagaataa ataactaaaa aaagacaggg tataaaagag
     2821 gctgtgacac gattttttgt cacagcctct tttgaatcta acaagcaata ttcaagcaac
     2881 actcgactga agagcttcca tcccaccaat gatataaaca gcacgaaaac cattagcagt
     2941 aagttgatcg gctaatgctt ctgatcgcct gcctgtatag cttagaatat aataaagttt
     3001 ctttggatct agatttttta attgtcttgg caagatagca gcggggatag aaagcgagtg
     3061 gcttaagtgg ttttttgtgt aatctaaggt atttcttgta tctaaaacga tgctgtcagc
     3121 atcgagaggc aatttattga agtcttggac actaattgat tgggccattt atctcatcct
     3181 ttgtttagta tgtggctatt ttaatgggca actctcatta attcaaatgg aaggattcct
     3241 cttatcactt ttttaatgta tcggatgata atgaaaaaaa ctatgcaaat gtcttgcaaa
     3301 acaggtagga ggtgttataa tgacatagcg tcatatgaca atgtgtcatc ttgaaagaaa
     3361 ggagaatcca tatgccgaca cagacatttt tccatttgcc taaagaaaaa caaaaaagac
     3421 taatcgaggc tgcgagaatc gagttttcta gagtaccctt aaaagaagct tcgattgcta
     3481 atatcgtcaa attagcggat atccctagag gcagtttcta tcagtatttt gaagataaag
     3541 aggatcttta tttttattac tttgagacaa tgagacgtga cagctctaaa gatatgttac
     3601 aactaatgaa agaatccgat ggtgatctat tcaaaggatt cgaatcctat ttttctaaaa
     3661 tgatttatga gattttggaa ggcgagaatg cttcttttta tcgtaacttg tttatgaaca
     3721 tggattaccg tgcctcaaga aaagtcgctc ctcatacgag tcgccaaaaa acgcctcacg
     3781 atccacataa agaacacaaa caagatgcaa aagagttgct tgaattgatc gatcggacga
     3841 agctgaaagt aaaaaatgat catgaattgg aattactgat ccaattagtg atgaatactg
     3901 tatttacaac aattgcgcat tcgtatcgct tattttcaac atctgaagat tactcagtag
     3961 agcaagcaat cagtgaattc aatttgaaac tttcttggct gaaaaacgga gtatataaat
     4021 agaaagaagg gaactagatg ctaaaaatcg taaaacggat ctccttgact tcagcaattg
     4081 cagctgcagt tttcatggtg atccaagtac tggcagattt atatttgccg acacttacct
     4141 ccaatattat tgataaaggg gtagcgcaag gagatgtcga ttatatttgg catacaggtt
     4201 ttgtgatgat cggcttttct ttgatcagta tcttagctgc tatcgcaaat acattttttg
     4261 ctactcgtga atcacagaaa ctgggtaagc aattgcgtac ggatgtctat aaaaaatcag
     4321 agagtctgac aaaagatgcg tttgataaat atggaacggc ctctttgatc acaagaacga
     4381 ccaatgatgt gactcaaatc caaatggtta cacaaatgtt tttacgtatg atgatcaatg
     4441 caccaatcac gttgattggc gcgagcatac ttgcttatca aaaagatcat cagttgacaa
     4501 aaatcttctt agttgtcatc ccagtaatga ttattttgat cggcggaatc atgtactttg
     4561 ctgttccatt attcaaaagt atgcaaaaga aaactgaccg tttaaacctg gtatttcgag
     4621 aaggattgac aggggtcaga gtgattcgag cattcgataa gacacgcttt gaagaaaatc
     4681 gttttgattt agcgaataaa gattatacaa atacggcaat caaagttaat acgatcgtcg
     4741 cattgatgat gccaatgatg acattgatca tgagcggtac aaacgtagca atcacttggt
     4801 ttggtggaca ctacatagca gatatgactt tggaagtcgg taatctgatt gcatttatga
     4861 cttatgcgat gcagatcttg atcagcttta tgatgctttc cgctattttt atcatggtgc
     4921 ctcgtgcaca agcttcagct gaccgtatta atgaagtgtt ggatgaaaaa atcggtatcc
     4981 atgaccctga aaatcctaaa acagtttctt ttgctggaaa aaacgctaca ttggccttca
     5041 atcatgtcaa ttatcgatat catggagcag aaaaacttgc attggaagat attgatttcc
     5101 aagccaagtc gggcgagatc gtcgcaatca taggggggac tggttcaggt aaaacgacct
     5161 tagtcaattt gatccctcga ttatatgaca tcgaatctgg ttcgatccaa ataaatggaa
     5221 cggacattag tgacatgact caatataatt tgcgagaatt aatgggcttt gttcctcaaa
     5281 aagcagttct gttctctgga acgatccgag acaatatgca gtatggtaag ccggatgcaa
     5341 cagatgaaat gatttggaaa gcattagaaa tcgcacaagc taaagatttt gtttcagaga
     5401 tggaagatgg cttagatagc catgtggaac aaggaggagg aaacttctct ggtggacaaa
     5461 gacagcgtct ggcaattgca cgggcattag tcaaaacagc agatatctat gtgtttgatg
     5521 attccttctc agcgttggat ttcaaaacgg atgccaatct tcgccaagca ttgaaaacga
     5581 atatgaaaga aagtatcacc gttttagttg ctcaacgagt aagtacagta atggatgcag
     5641 atatgatcct tgtgctagat gaaggaaaac tggttggcaa aggaacacat gaagaactat
     5701 tagcaacaaa cgaaacttat caagaaatcg ttcattcaca actaagagag gaggacttag
     5761 catgagtaaa acaaacacac gtccctctca tggaccagca ggcggtccag gacatggtgg
     5821 acctggcaga aatctcggtg taaaaggtga aaagcctaaa aatttctggg gaacggtacg
     5881 ccgattgttt ggctatatgt ctaaacgtat ggttgcgatc atcgctgtat ttgtactagc
     5941 aatagcagcc gtgatcttcc aaatccaaac accaaaggtc ttggggaaag caaccacaga
     6001 aatattcaaa ggtgtaatgg ctggcgctca acagatgcaa caaggccaac aagtaacaaa
     6061 atttccgata gactttgata aaatcgcgca gatcattgcg acagtaatcg tgatgtattt
     6121 gatttctgct gtattcaact ttttacagca atttattatg acacgtgttt cccaacgaac
     6181 agtttatgaa ttacgtcatg atttggaagc caaaatgaat cgtgtaccga tttcctatta
     6241 tgatacacat acaaatgggg atatcatgtc tcgagcaatc aatgatatgg ataacatcgc
     6301 aagcacgctg cagcaaaatt tgactcaatt tgtaactagt gcagtcacat tgattggggt
     6361 catcgtgatg atgctttcaa tcagctggca attgacgtta gtcgcgttat tgatgatccc
     6421 tttaagcttg atcatcgtga tgatcgtagc gccgaaatca caagtgtttt tcgcagatca
     6481 gcaaaaaagt ttaggtttat tgaataacca agtagaagaa acatatggtg gtcataccat
     6541 tgttaaaaca ttcaatcata ctgaaaaaga ccaggaagta tttgaaaaag aaaatcagaa
     6601 tctgtatgcc gctggttgga aagctcaatt tatttcggca atcatcatgc cgttgatgaa
     6661 tttcgttaaa aatctaggct atgtgttcgt cgctgtacta ggcggagtaa aagttgcaaa
     6721 tgggaacatg acattagggg atgttcaagc gttcttgcaa tatacaacac agttctctca
     6781 accaatcact caacttgcaa gtttgatgaa cactatccaa tcaacagttg cttcagccga
     6841 acgggtattt gaagtgttgg atgaagaaga gatgtccaat gaaaaatctg gccttccaga
     6901 agaaaaagat acaccataca aagttcgttt tgaaaatgtt caattcggct acacaccaga
     6961 taacttgcta atgactgatt ttaacttgga tgtcaaacct ggtgagatgg tcgctattgt
     7021 aggtccgact ggtgcaggga agacgacact gatcaacctt cttgaacggt tctacgatgt
     7081 ttctggaggt agtattcgtt acgatggtgt agatacccgt gatctttcac gtgatgaatt
     7141 gcgcgcacag ttttcaatgg tactgcaaga tacttggctg tttacaggaa gtatctatga
     7201 caatatcaaa tatgggaacg acgatgcaa
//
LOCUS       DACEXQ010000137         7157 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.137, whole genome shotgun sequence.
ACCESSION   DACEXQ010000137 DACEXQ010000000
VERSION     DACEXQ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.137"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..1138
                     /gene="mutY"
                     /locus_tag="IXH04_001956"
     CDS             <1..1138
                     /gene="mutY"
                     /locus_tag="IXH04_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAQ1852548.1"
                     /translation="EFQDQFIQWYEQEKRNLPWRYNRDPYRIWISEIMLQQTRVDTVI
                     DYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAAKQIMSEFDGEMPQT
                     PEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCIEADIAKASSRKIFD
                     EAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCLANKRGIQTSFPVKT
                     KKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEVTQEEYERLKKEWET
                     KPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLKWHVLLFYGRATEGA
                     EQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF"
     gene            1232..1765
                     /locus_tag="IXH04_001957"
     CDS             1232..1765
                     /locus_tag="IXH04_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAQ1852549.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(1843..2277)
                     /locus_tag="IXH04_001958"
     CDS             complement(1843..2277)
                     /locus_tag="IXH04_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1852550.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            2419..3537
                     /locus_tag="IXH04_001959"
     CDS             2419..3537
                     /locus_tag="IXH04_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1852551.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            3665..5041
                     /locus_tag="IXH04_001960"
     CDS             3665..5041
                     /locus_tag="IXH04_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ1852552.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            5442..7022
                     /locus_tag="IXH04_001961"
     CDS             5442..7022
                     /locus_tag="IXH04_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAQ1852553.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
BASE COUNT         2341 a         1219 c         1493 g         2104 t
ORIGIN      
        1 agaatttcaa gatcagttca ttcaatggta tgaacaagaa aaaagaaacc tgccttggcg
       61 ctataatcgt gatccttatc gtatctggat ctctgagatc atgcttcagc aaaccagagt
      121 agacacagtg atcgattatt tttatcgttt catggaatgg tttcctacaa ttgaagagct
      181 agcaaatgct ccagaagaaa agcttttaaa agcatgggag ggccttggtt attactcaag
      241 agcccggaac atccaagctg ctgctaaaca gatcatgtca gaatttgatg gggaaatgcc
      301 gcaaacgcct gaagaaatta gctcattgaa gggaatagga ccatatacga caggcgcgat
      361 tgcaagtatc gcatttggac ttccagaacc tgcagttgac ggcaacgtga tgcgagtagt
      421 cagcagattg ttttgcattg aagcagacat cgcaaaagct tctagcagga aaatctttga
      481 tgaagcgatg cggaaaatca ttgatgaaaa gcatccaggt gaattcaacc aagcaatgat
      541 ggatctaggt tcagctatct gtacaccaac ttctccaaag tgtgagactt gtccgatcca
      601 agccttttgt ttagctaaca aacgaggaat ccaaacgagt tttcctgtaa aaacaaaaaa
      661 agcaaagcca aaagacgttt attatatcag tgcagctctt caaaatcatt ctggagcgta
      721 ttattttgaa gaaagagata gccaaaaatt gctggcgaat atgtggacat ttccaatgat
      781 ggaagtgaca caagaggagt atgagcgatt gaaaaaagaa tgggaaacaa agccagaaat
      841 cgatctgttt gatgacctag tagcagaaga agagcaaaat ctcccatttg aaaagcagga
      901 actctttgtt tggcagacac gacatttagg agaagttact catattttta gtcatctgaa
      961 atggcatgtt ctcctttttt acggacgggc aaccgaggga gcagaacaag aatttaccga
     1021 gaacaaaact tcgaagtggt tgaagcctga tgcatttgat tcggtcgttt ttcctaaggt
     1081 ccaaatgaaa ctggtagatc agttagagaa aaatcgaaac aataggaatc ctttctaggc
     1141 aaagctcatt tttattgata taataattat gatgtcggtg taatgaacac ccaaacacaa
     1201 agcacaaaat gctgcttaag gaagggttgc tatgggagtt ccaaaagaag gagagtttgt
     1261 gacgatccaa agttacaagc acgacggcaa attgcatcga acgtggcgag ataccatggt
     1321 attaaaaaca agcgaacact ctatgatcgg cgtgaatgat cacacattag tcactgaatc
     1381 tgatggacgc cgatgggtaa cgcgcgaacc agcgattgtt tattttcata aaaaatactg
     1441 gttcaatgta atagcaatga tcagagaaaa gggggtttcc tattattgta atctagcttc
     1501 accttttttg ctggacgatg aggccttgaa atatatcgac tatgacttgg atatcaaggt
     1561 atttcctgac ggcgagaaac gactattgga tgtcgatgaa tatgagatgc acagcaaaat
     1621 gatgaattac ccgaacgata ttgacttcat cctaaaagaa aatgtgaaga ttctggttga
     1681 ctggatcaat aatggagatg gcccgttctc ggaaggctat attgatattt ggtataaccg
     1741 ttacaagcaa ttgtcgcgta agtaaacaca aaaaagaaca gtaagccagt gagagatttt
     1801 tctcaaaagc ttgctgtttt ttgctttttt cgaaataaat ttttataaag tctttttcat
     1861 ataaatatga ggcatgccag catcttcaaa aggatcgccg taagctgtat atcctagttt
     1921 ttcgtaaaaa gtttcagctg tggactgtgc ccctaataaa atcgtattat agccttgatt
     1981 cttagcaaag ttttctgctt cttcaatcaa tatttttcct aacccttggt gtctgtattc
     2041 agaaagaatc gccatccgct gtaatttcat ttttccgttt tctaaaggca aaagacgtac
     2101 agttccttgc ggttcttttt tatctgagta caggacgaag tggatacaat gggcttcatc
     2161 tttgtctatt tctcgagaca aaggcacacc ctgttcgact acaaaaactt gatgcctgat
     2221 ccgtacagca tctaagtaaa tattgctcat tgtatctttt gtttgtacaa ctttcatggt
     2281 ctagccacct tcctttcgta tagtatactg gaaaataaaa caaataaaaa gtatggcaag
     2341 ttttcaaaaa taaaaaaaga ataaaagaat ttttaacgaa tataatatat actagtaaga
     2401 aagaaaggag gaaatctatt gtttgaaaaa cttagacagt ctaaactaat gttttggtcg
     2461 cttgaattat tgatcatagc tgcattgatc atgatttcat cagaaataaa ctttattttc
     2521 gaaccgatcg ggacattttt ttccacgtta tttgcacctg tactgattgc aggctttctt
     2581 tactatttat tgaatcctat tgtcaagctg ttgatgaaag caaaaatcaa aagaatatgg
     2641 gctgtcgcaa ttgtgctgct ccttttagtt gcagcaatta tttggatttt acttagtgtc
     2701 atcccaagtc tagtacagca aatttcctca ttggcatcta atatgcctga tttcatcaaa
     2761 caggtagaaa gctggttaaa agaggtagcc gaattgcctt tgttcaagga agtagatatc
     2821 aataaatatt ttgagcaatt agacatttct tacgggacaa tcattcagca attccttagt
     2881 ggagtatcca atagtttagg ttcgatcgtt ggaacgatcg cttcggcaac cgtagtaatc
     2941 attacagcac cattcatctt gttctatatg ttgaaagacg gagaaaaact agtgccaaac
     3001 atccaacagt ttttcccaga aaaaagaaga aaccaaattg tggagttatt ggggcaactg
     3061 aataaaacat tggctaatta cattagcgga caagcaatcg aatgtttatt cgttggtacc
     3121 ttcacatttt taggatatat ggtaattggt gtggattatg ctttcttatt tggggtcatt
     3181 gcgggattga caaacctaat tccatattta ggaccttatc taggactagc acctgcattt
     3241 ctagtaacag tcttcaatga tccggtaaaa gcattacttt gttgtgtggt tgtgctaatc
     3301 gtccaacagt tggatgggaa tatcatttat cctaatgtaa ttgggaaatc actgaaaatc
     3361 catccattaa cgatcattat cgttcttcta gtagctggaa atattgccgg attgcttggt
     3421 atcttcttag gtgttccttt ttacgcaatc tgcaagacga tcatttccta tataatcaaa
     3481 atcgtcaaag aagacaagca aaatgaaaac aagaagaaaa ttgctacgac ctcgtaggta
     3541 gttttagtac acttgttttt attgcaaaga aaattgcttt gtgataccat tttaacgtgg
     3601 ctatagtgcc acgttttttt ctatcgtaaa ctaaaaatta attttataag gagagaatgc
     3661 gattatgaat gctgaccctg agagtcagtc gctgttagcg cagattttat tattgatcgt
     3721 tttgacattg atcaatgctt ttttagcagc ttccgaaatt gctgttgttt cgatcaataa
     3781 gaatcgaatc gagcaaaaag ctgatgaagg agatgtgaaa tctcaaaagc ttttaaaaat
     3841 attgcagaat ccaaataatt tcctatcgac aatccaagta ggaatcactt tggtcaacat
     3901 tttgtctggt gcttcactag ctaatacttt atcggaacgg ttagctccgg tattaggtgg
     3961 cggcacagcg gcaaaaaata ttgcaaatat cattgtgctg gctattttga cctatgtatc
     4021 gattgttttt ggagaattgt accctaaacg tattgcttta aataaatcag aagaagtcgc
     4081 aaagttcact tcagggctga tacgcttgat tggtgtagta gctaagccct tcgtctggct
     4141 gctttctgct tccacaagtt tattggcgcg tatcacgccg atgacttttg atgatgaaga
     4201 ttcaaaaatg accagagatg aaatgcgtta tatgcttgaa aacgaaggcg tattgaataa
     4261 tgaagaatta gaaatgctcc aaggggtttt ttctcttgat acgaaagttg cccgagaagt
     4321 catggtccca cgaacggatg catttatgat tgatatcaac gacactgtgg aagaaaatgt
     4381 gaatgaagtt ttatccgaaa actattcaag aatccctgtt tataacgaag acaaagataa
     4441 agtagtcgga atccttcata caaagaattt actaaaagct gcccataaat tcggctttga
     4501 taatttagat atcaagaaaa tcatgcagga gcctttattc gttcctgaaa cggtctttat
     4561 tgatgatctg ttatatgaaa tgaaaaagac gcagaatcag atggctattt tattagatga
     4621 atatggcggc gtcgttgggt tagcaacact agaagacctt ttagaagaaa tcgtcggaga
     4681 aatcgatgat gaatcagatg aagttgagaa tctttatgaa aaaatcgatg aacacgaata
     4741 tatcatccaa ggacgtatgc tgattgatga gttcaatgaa gcttttgaca gtgatctgca
     4801 tatgagtgat gtagatacga tggctggtta tttgattaca gcattaggca tgattccgga
     4861 tgaaggcgaa aagttatcgt ttgatgtcga taatattacg ctggtttctg aagaaatgga
     4921 aggttctcga atccttaaaa tccgtgtcat ttttcatgat ccggaagaaa cagaagcgga
     4981 acctgaagaa gaacgaagat acttcagaaa agaattcgaa gatgacgagc caagaagata
     5041 attggaaact tggttatgaa agacgtgttg cttgaataaa caagcaacac gtcttttctt
     5101 ttagtcagct gacaaaaaaa tatttgactc ttatggtttg gtggcttttt cattcgctga
     5161 atccatgcta tactgattca gttgtagtag ttgacaaagg ttgtgaaaaa gctatcctgc
     5221 attaagtaat aagagcagcc aagcaaaaac agcttttcgt atttttggct tgggtgggct
     5281 tattgctgat aggctgtctc tagaacgccg tttactcgga aaaagagact gctacacggc
     5341 ttttgtcaca gccctatatg aaattgtctt tctttaagag atcggacaat ctgtgcctag
     5401 agaaaatata aataattgaa tggaagaaat ggagttaggt tatgaataat ccaaaattaa
     5461 agcaacaagt ggacaatcgc agaacttttg cgatcatttc ccatccggat gctgggaaaa
     5521 caacgattac agaacaatta ctattgtttg gtggggctat ccgtcaagcc gggaccgtaa
     5581 aaggcaaaaa aacaggtaat tttgccaaat cagactggat ggaaatcgag aaacaacgtg
     5641 ggatttctgt tactagttct gttatgcagt ttgattatca agggaaacga gtaaatatct
     5701 tggatacacc aggacatgaa gatttttctg aagatactta tcgtactttg atggccgtgg
     5761 atagtgcggt catggtaatc gatagtgcaa aagggatcga agcacagaca aagaaattat
     5821 tccaagttgt aaaaaaacgt ggcatcccga tatttacttt tatcaacaaa ctagatcgtg
     5881 atggtcgtga accattggaa ttgttagaag aattagaaga gctattagat atcgaatctt
     5941 atccgatgaa ttggccaatc ggtatgggta aagggctaga aggtctttat gatatctacc
     6001 ataatcgtgt agagttttat cgtccggaaa attatcagga tgaacgatta gtagaattgg
     6061 atgaagatgg actgcttccc gaaaatcatc ctctaactga aaattcctta tatgaacaag
     6121 tcttagaaga agtagaacta atcaaagagg ctggagatgc atttaatcaa gaaaaaattg
     6181 ctcgtggcga tcagacacca gtattttttg gttctgcatt gactaacttc ggtgtacaga
     6241 cttttttaga gactttcgtt gaattggcac cagctcctta tggacacaaa acacaagatg
     6301 atcagatggt aagtccttat gaggaagaat tttcaggttt cgtattcaag atccaagcaa
     6361 atatgaatcc agcccatcgt gaccgaatcg cttttgtccg aatctgttct ggaacattcg
     6421 aacgaggaat ggatgtctgg ttggaacgta caaataaaaa actgaaatta agcaatgtca
     6481 cacaatttat ggcagattcg agagaaaacg tcgaaaaagc agtagcaggc gatatcatcg
     6541 gtgtatatga tacaggcaat tatcagattg gggatacatt gttcgaagga aaactaaaag
     6601 ttgcttatga agaactccct tcatttactc cagagctgtt tatgaaagtg actgcaaaaa
     6661 atgtcatgaa acaaaaatcc ttccacaaag gaatctatca attagttcaa gaaggagcga
     6721 ttcagcttta taaaacatat ctaacggaag aatatatcat tggagctgtt ggtcagctac
     6781 aattcgaagt attccaatat cgtatgttaa atgagtataa cgccgaagta atcatgtctc
     6841 caatgggaca caaaatcgct cgctggatcg atccagaaga tttggatgag aagatgagtt
     6901 caagtcgcaa tatcttggct cgtgaccgtt tcgaccaacc gttgttctta ttcgagaacc
     6961 aatttgcaga acgctggttt gcggataaat atcccaatgt aaaattaaaa agtttgatgt
     7021 aaagaagtct tgacacaagc tgccctgcat caagtaataa gaatagccaa acaaaaacag
     7081 cctctcatat tttgtttaga gccgtgacgg attttccgtg acggctcttt ttactatatt
     7141 aaaatcatag aaatgtc
//
LOCUS       DACEXQ010000138         6952 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.138, whole genome shotgun sequence.
ACCESSION   DACEXQ010000138 DACEXQ010000000
VERSION     DACEXQ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.138"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            25..588
                     /locus_tag="IXH04_001962"
     CDS             25..588
                     /locus_tag="IXH04_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ1852554.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            601..885
                     /locus_tag="IXH04_001963"
     CDS             601..885
                     /locus_tag="IXH04_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852555.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            904..1596
                     /locus_tag="IXH04_001964"
     CDS             904..1596
                     /locus_tag="IXH04_001964"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAQ1852556.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            1608..2153
                     /locus_tag="IXH04_001965"
     CDS             1608..2153
                     /locus_tag="IXH04_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1852557.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            2424..5702
                     /locus_tag="IXH04_001966"
     CDS             2424..5702
                     /locus_tag="IXH04_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAQ1852558.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(6243..>6952)
                     /locus_tag="IXH04_001967"
     CDS             complement(6243..>6952)
                     /locus_tag="IXH04_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAQ1852559.1"
                     /translation="DQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAY
                     YHPFRNVIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNL
                     NNWWTEEDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKT
                     ESDLSIEEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGI
                     TEQDAMYLAPEKRVRIW"
BASE COUNT         2347 a         1270 c         1483 g         1852 t
ORIGIN      
        1 attacaaaga aagcaggaga agatatgctg aacaatgagc aatttgaaga aaaaacaatc
       61 agtcgcaaag aaatctatag cggaaaaatt attgatgttg ctgtagacga agttcgtttg
      121 ccagatggag gaacttcgaa gagagaactg gtatttcatc ctggcggtgt cgggatcatt
      181 gcttttgacg aacaagatcg tctgcttctt gtcaaacaat ttcgaaaacc tttagaaaaa
      241 gtgattttgg agattccagc tggaaaaatc gatccaggag aaggacaaaa tcccgaaatg
      301 acagcagcta gagaattaga agaagaaact ggctatcgtg cgaagtcgct gagccatctt
      361 acttcgatgt atctttcacc aggttttgca aatgaagtcc ttcatattta tcatgcacaa
      421 ggggtagaga aggtcgagaa tcctttagca caagatgaag acgaagtact ggagttgtat
      481 catctaactc ttgaagaggc gcaacaagcg atgaaagatc agttgatttg tgatgcaaag
      541 acgatttatg cgattcaata ctgggaacta ttgaccaaag gaaagtagag ggatcggctt
      601 atggccaaag gaccgcttat tacgcgaagc gaacttcgca agcgacaaca agcacaagca
      661 agcgagtcat taaaaaaaca gcgaaaagca gaaacagctt atcagcaaga agaaaaaaag
      721 attgccagct tttatcgtaa agaaagcaaa aagaataaac caatcacgaa aacaaggatc
      781 agcgaacggg aaaagacgac caaatggaac tcttttttga tgaaatctct tattattgtt
      841 atcctaatgt tatgtgtggt ttttttagca atcgcattta tatagaaaga aggaattaaa
      901 aagatgaaaa tcggtattat cggcgcaatg gaagaagaag taaaaatttt gagagagaac
      961 ctaagcgaac cattatcatg ggaacgcgca ggcgctttat ttatttcagg ttcattaggt
     1021 aaccacgaag tgatcgtcgt acgttccggt ataggaaaag tcttagcatc tattaccaca
     1081 agtctgttga tccaacaata cggcgtgaac atggtgatca atacaggttc agctggcgga
     1141 atcggtgaag gcttacaagt aggtgacatc gttatatcag acaaagtagc ttattttgat
     1201 gcagatgcaa caggatttgg ctacaaccca ggacaattac caggaatgcc tctatattat
     1261 gaagcaagca catatttgcg cagtgaaatg gttaaagcag caaaagcaac gaatttgaac
     1321 gcaaaagaag gattgatcgt tacaggtgat acatttgtcg actcgccaga taaaatcaaa
     1381 gaaatcttaa cgaatttccc tgaagcatta gcttgcgaaa tggaaggagc cgctgttgga
     1441 caaacagctc gtcagttcaa tatcccattt ttgatcgtac gtgcaatgag cgacacagca
     1501 gatcactcgg cgacacaaag ctttgacgaa tttatcgaag acgcaggcaa acgatcagca
     1561 gaaatggtga tcgaatttgt caaacatctc gtataaggag gactaagatg aacgcattga
     1621 tctcgattga ctacacatat gattttgttg cagaggatgg aaagctgact actgggacag
     1681 caggacaagt aattgaaaaa aagctgactg ctcatacaaa gaagtttatt gatcaaaagg
     1741 attttgtcgt ttttgccatt gatgcgcatg atccaaaaga ttcctttcat ccggagaacc
     1801 gcttatttcc tccgcataat gtgataggaa cgggtggacg ggaattgtac ggctctttaa
     1861 aaagtttata ccaagactat gagcaagagg aaaatgttta ttggatagac aaacggcatt
     1921 attccgcttt tagtggaaca gatctagaca tccgtttgcg agaacgccag atcacagata
     1981 tttatttgac cggcgtatgt acagatatct gtgtgttgca tacagcagtc gatgcgtata
     2041 atctaggata caaactccat attttcaaag atgccgtggc aagtttcgat cccgttggac
     2101 atgaatgggc attgagacat tttgagtcca cactaggcgc agaaatttta taaaaaaaca
     2161 aagtgatttg ataaaataaa aagcacaaaa taataaaaga agagcataaa acaaacgact
     2221 ttgttttatg ctctttttta tacgttattt taaaaaaact tattcttttt acgttaataa
     2281 atctctttat tatcaacaac ttaagttata ttttttcttt ttataaaata cacttattat
     2341 aattttgttt tttattgaca gtaaaaataa attaggtaat actttaagta aacgctttct
     2401 aatttgttac gggaggaata cacttgttaa aaaaacgtat aaaatacagt gcgctaatga
     2461 tgaatctcct tatgctcagt accccggttt taatttctgt cgttgaagtt gctgctaacg
     2521 aacaacagtc tgtgaacgaa gaaaatgcac acgtgaacat tgaagggatg tcaacaatgg
     2581 aggaaatacc aagaactagt gaagatatgg atacggaaga aggaaatgat cctatgatga
     2641 aagaatcaga aaatgaaaag acaaaaaagc aatctgaaga agcaaatgaa gagtggaaag
     2701 gggttgtttt cggagaaagc acaagtgcta gtaggtatgc cattgaacca ttagaagacg
     2761 gcgtgcggct ctcttccacg aataacggcg gaaaattcca gtcaagcgga tcagatggaa
     2821 tgacctatta ttatcaagca atccccaaag acatcaactt taggatgcgg gcgacgatcg
     2881 aaatagaatc ttggacctat acgaatgccc aagaaggatt tgctttaatg gtaagagacg
     2941 cagtaccaaa aaatcatttg tttgggcagg cattttattc aaattcattt gccatactag
     3001 gcagtcggat cgaatatgtc tgggactcgg atagacaaga agtggtcaac acgagcggca
     3061 gcaaatatac gatgcgactt ggattgggga cacgggcaat cacaggaata tcatctgaag
     3121 atccgcaagc tgcgccagct cctgggagtg tctcagtgga aacaacacct ttagagactt
     3181 cagcagggtt cttggaaaaa gaaacaggtt cttataatat attcggaaac ggtcatggga
     3241 atctgtttcc ggccacgaac tcgattacta aactggacgt agagatgaaa aaaacgaata
     3301 ctggtttaga ggcatattat tacgatcctg aaacaggaga agaaatggct tctgacatca
     3361 tgtatgactg ggaaaaatta tacgcttcag atgaatcggt tatttatgca ggatttgctg
     3421 cagcaaggaa tatgacgatc aaggtagaag gaatcgaagt tgcttactca gatcctgcga
     3481 cagatcctcc tgggcaagaa agacctactc gcttgattga tccgatttac actgtgacct
     3541 ctagtaatca ttcaggaaac caagatcata ctatgtcctt tagagcaaat gctgacggat
     3601 tattgacaat taaaaataaa gcaacaggag aggtgcttaa gaacgcaaaa aacttggcta
     3661 tcactgcgaa tcgggaattt gattatcaga caaaactgat ggaaggaacg aacgaattta
     3721 ctttcagttt tacgccagat aaaaactatc cgccagaaga atacgtagag atgactagtt
     3781 atgaaacaaa agagattctt catacagtgg actaccgtaa acctaaatat tcgacagttt
     3841 atgtaacacc agaagggtca gatgcaggaa acggaagcag acaaaatcca ttatctctga
     3901 cacaagcatt gagatacgca tatgctgggc agacaatcgt catggcgcct ggaacttatt
     3961 ccttcgaaag aggattgacg attccaaaag gcgttaacgg gacaagagaa aacccgattc
     4021 agttgattgc agagaaaaat ccagaaaaca agcgtcctgt acttgatttt aaaggaactg
     4081 gaaatgggat gacactgttc agtcactact ggggtttacg agggtttgac attacaaata
     4141 gtgcagctat gcaaaaagga ttacagattt ctggaaatca taatttgatc gaagagattc
     4201 atgcttatcg gaatggaaat acaggaatcc aaatcagcgg tagctcaaat gatcctttcg
     4261 aagcatggcc cgcgcataac ttagtcaaga actgtacatc ttttgaaaat gccgatccag
     4321 gatttgaaga tgccgatgga tttgcggcca aactgacaat aggagaagga aatgtatttg
     4381 acggttgtat cgcgtatcat aatgcagatg acggttggga cctttttgct aaaaatgaaa
     4441 caggttcgat cggaaaagta atcatcaaaa actcagttgc ttatcgaaac ggctgggtac
     4501 caggaattga gggagaaggt aacggtaatg gattcaaaat gggcggttct tctttaacgg
     4561 gccctcatga attgatcaat agtttgtctt tcgaaaatct tgcgaaagga atcgattcca
     4621 atagcggaac agatattatc gtcaatagca gtacaagttt taataacggt tcatataatg
     4681 tggctcttta tacctcttca gcaggaaaca cagactacca tgcttctggt atcttatctt
     4741 tccgaacaga gaatcttgag atgagagagc agatccgccc attgaatcaa aacgaagatc
     4801 aaatctacca ctcgttgaac tactattgga atgaacgaga gaaacaatca gaaaatactc
     4861 ttggacatac catatcaaaa gattggatcg agtctctaaa tacatgttcg aaagaaggtc
     4921 agcagcctgt tacgcgctat gaaaatggaa gtatccatgt tcatggactg atgcagatta
     4981 aaccggggaa tcgtcaaaca gaaaaggaaa gagtggatgg tcttccatta tctgtaggtg
     5041 ctatttttga cggagaaacc tcaccgtctt cggaatatcc agagtatgca atcgtacaag
     5101 aaccaaatca gacaaaacca aatgaacaaa ataatccgat tattatacaa gaagcaaatg
     5161 gtgtggcgga gaatgcagaa ggaaatatca ttgaactgaa gccttttaca gtttttgtgt
     5221 caactttcac accaaatcaa ggaacgcctg tctttgaaga tatgcgttat ttcccagaaa
     5281 agatccaaat cgttgaaaca cagtcaagtg ctcaattttc taatcatacc gctatttttt
     5341 cagcaccttc aaaaggagaa tatacgctag aagtcactta tcgttcggaa atatttgatg
     5401 gggaaaaatg gtctgctcat acagaatctg ttacccagca aaaagcgatc actgtgcgag
     5461 aaactgccga tgtgcccccg ggaaatgaca aaccagaaag agaaaatcca ggaaatgaat
     5521 caccaaatgg aaaaccaaat ggaaaacctt ctcatccgga taggacagca aaagaaagtg
     5581 aaaaaaatgg cactcaagga ctcccaaaaa cgggagaatt taataacccg ttgctagcat
     5641 tagctggagg aattttgtta atcggtgtag ttgtttatgt catgaaacag cgaaaaaaat
     5701 aagtggaatt tctcctttta tgatgtaaaa caattgactg aggaaagtaa aaatagaagg
     5761 aaatgagacc aatagatgtc tcacttcctt ctatcttttt atagatgagg ttctccttgc
     5821 tgcttttgat tggctgttct tattacttga tgcaggacag ctttttcaca atctctccac
     5881 taaacggtgt tcaatcagct gaccctcggt attagctcaa caaacggcaa aatatgagga
     5941 actatttttc cgtttttttc tctaatactc aggtcaggac gctgatttca caacctctcg
     6001 ataaacggtg ttcaaaagcc agtttctcgg tagtaagtca aatttatgca aaaatgtgga
     6061 aagccatttt cacaaaattt ttcttactac ttgaaactaa gcagcttttt cacaacctct
     6121 aatacatggt attccagaag taacttcttc gaaaaataag ccgaaatgtc acaaaaattt
     6181 gagaagcgat tttcccaaat tccttcttat ttcttgaagc tgaccacttc tggcacaacc
     6241 tcttaccaga ttcgcactct tttttctggc gctagataca tagcgtcttg ttccgtgata
     6301 ccaaatgttt catagaattc gtcaaggttt tttggttgca cgtttgctcg gagtttagca
     6361 ggggcatgga catcgatttg cagtaaaagt tgttggtatt ctttttttgc ttttgtacgc
     6421 caaatcgttg cccaattgac aaagaattcc tcgatagaaa gatctgattc cgtttttgct
     6481 gcttccaatg cacagcttag accgcctgca tcagcaatat tttccgaaac ggttaatttt
     6541 ccgtttactt ttccgtcagc aaaaggcaga ccatcgaatt cttcgatcat tgcttgtgct
     6601 ttgttttgaa attgtgtaag atcttcttct gtccaccagt tgttcaagtt gccatattcg
     6661 tcaaataaag cgccgttatt atcaaatgca tgggaaatct catgagcgat aacagctccg
     6721 attccaccat aattagcaga gcttgattgt tctagactgt aaaatggtgc ttgaaggatc
     6781 gctgccggaa agacgatcac attccgaaat ggatgataat aagcattgac cgtgtcagca
     6841 ctcatttccc attctgaacg atccactggt ttgttccatt tgctgaaacg atcttttaat
     6901 gtgagtttgc tgaaaacaag agtatttgaa agcaaagtac caccctgatc tg
//
LOCUS       DACEXQ010000139         6909 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.139, whole genome shotgun sequence.
ACCESSION   DACEXQ010000139 DACEXQ010000000
VERSION     DACEXQ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6909
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.139"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            142..1032
                     /locus_tag="IXH04_001968"
     CDS             142..1032
                     /locus_tag="IXH04_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAQ1852560.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(1105..1929)
                     /gene="nadE"
                     /locus_tag="IXH04_001969"
     CDS             complement(1105..1929)
                     /gene="nadE"
                     /locus_tag="IXH04_001969"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAQ1852561.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(1941..3413)
                     /locus_tag="IXH04_001970"
     CDS             complement(1941..3413)
                     /locus_tag="IXH04_001970"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAQ1852562.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(4050..4412)
                     /locus_tag="IXH04_001971"
     CDS             complement(4050..4412)
                     /locus_tag="IXH04_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAQ1852563.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            4635..5387
                     /locus_tag="IXH04_001972"
     CDS             4635..5387
                     /locus_tag="IXH04_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1852564.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(5527..6450)
                     /locus_tag="IXH04_001973"
     CDS             complement(5527..6450)
                     /locus_tag="IXH04_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ1852565.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(6465..6737)
                     /locus_tag="IXH04_001974"
     CDS             complement(6465..6737)
                     /locus_tag="IXH04_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852566.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         2130 a         1360 c         1166 g         2253 t
ORIGIN      
        1 taatataaat gttaaactta cttatttttt atcgatttta attaaccaaa atattaataa
       61 tgattaaaac aaataattta atataacttt tattaacaaa tatgttaata tataagaaaa
      121 accttttaag aggtgatata tatgcaacta caaataaacg aagaatcctt accggtatat
      181 gaagcattag ctagtaaaac aaggatcaag atcatccagc tactttccaa aaaaaagatg
      241 aatgtcaaag atttggcaaa agaacttgga gtgagcagtg ctatcacgac gatgcacgtg
      301 aaaaagctag aggaagcaaa catcatcaaa acagaaaaag taggacaaca aaaaatttcg
      361 agccttcgtg tcgataaaat cgatatcagt tttccagaaa aaatattcaa tgcttttgat
      421 acgaaagaaa catcgatacc tattggtcac tatacaaatt atgcaattga accaacatgc
      481 ggtttagcta cgatccatga ttttatcggg aaagtggatg aaccacgcta ttttatggat
      541 cctcgaagga tggatgcccg cattctctgg tttaccagcg gatttgtcga gtatcaggca
      601 cctaattttt taaatacaga ggatacattg gagatgttgg aagtttctgt ggaaatttct
      661 tctgaatttc ctttttcaaa tgacaattgg ccttcagata tcactttcag cctaaatggt
      721 gtggagctag gaacatggac gagccctgga gattttgctg atatccgcgg taaatatacg
      781 cctgattggt atccagataa tcttaatcaa tacggcttgc tgaagacgat ccgaattacc
      841 aaacacttga cgaatatgaa tggagaaccc ttgtcaaaca tcacgatcaa cgacatccct
      901 aaagagcaag acacttggca tttgcgaatc gaagtgaaag acgatgccaa acatgtgggg
      961 ggctgtactt tatttggtaa aggattcggt aattacgatc aggatatcaa attaaaagtt
     1021 tactacagct gatatttttc ttttctattt tataaaacaa aaacagccaa tccctatgaa
     1081 gggacggctg tttttgcttt attgttattt ccagaaatca tccaaaacgg aaattggcaa
     1141 atgtcgttta tgttgtgtct tgttccacca tgcttcgatt ttttcttgtg cttgagcggg
     1201 aacttctttt ccttctagat aatcatcaat ttcattatat gtgacaccta gtgcgacttc
     1261 atctgcaatc attggtttcc catcttcaag atctgcagtc ggaatttttg tgtataattt
     1321 ttccggtgca ttcaatgctt gaagcaattg tttcccttgt cgtttgtcca aacggaataa
     1381 tggcaaaatg tcggctccac catcaccaaa tttcgtgaag aatccagtaa tgttttctgc
     1441 agcatgatct gtccctaata cggctcctgc tctttctcca gctacggcat attgcgtaat
     1501 catacgttga cgtgctttga tatttccttt gttaaaatcg ctgattccca cacctgcttc
     1561 gcttaatact tgtacttgtg catcaacagc tggtttgata tttactcgca gactaacatc
     1621 gggctggata aagtcaagag cagcttttgc atcttcttca tctgcttgtt caccataagg
     1681 aagacgaaca gcgataaatt gatagttttc atcttttgtt tcttcacgca tttcttccat
     1741 cgttagttgc gccaaacggc ctgctaaagt agaatcttgg ccgccgctga tacctagtac
     1801 aaaagtttta agaaacggat attttttcaa atatgctttc ataaagtcaa tacttttacg
     1861 tatttcttct tttggatcaa ttgttggttt gacacctaat tcattgataa tttctgtctg
     1921 caggctcatt tattctcctc ctatgcttct gtatgaagtt ctgctacgct ttttttcatt
     1981 cgttccatga tagccatttt atggttccag cattcagtag acagatcgac tgggtatttt
     2041 tgcggattca agtctcgttt atactcttcc catagtgaat ctagattttc ttttgtatat
     2101 tctttgattt cgtttagtgc aggtaattcg taaactcttt ttccttcgat aaagatatct
     2161 tgtaaaactg ggcgagctgt aaagtctcga acagttttat tgataaacgt atgcactgga
     2221 tggaacatga agatagcttc ttcttctcga gggtcttcat cccataatgt cacgtagtct
     2281 ccttcagatt tcccatcaaa atttcgagtg atgcgccaca cttgtttttt ccctggagta
     2341 gttacttttt ctgcattgct tgataacttg atcgtatcca tcatttttcc ttcatcatct
     2401 tcgatcgata ctagcttgaa tactgcacca agggctggct gatcataggc tgtgatcaat
     2461 tttgtcccaa caccccatac atcaatttta gctttttgca tctttaaatt caaaatcgtt
     2521 gcttcatcca agtcattcga agcatagatt tttgcttccg taaatccggc tgcatctaat
     2581 tgttcccgaa cacgcttaga tatataggcc atatctccac tgtcgatgcg tacgccttgg
     2641 aagttgattt tgtcacctaa ttctttcgcc acgcgaatcg cacttggcac acctgatttc
     2701 aacgtatcat aagtgtcgac taagaacacg cagtctttat gggttttagc gtaggccata
     2761 aatgcttcat aatcatttcc ataagattgg actaacgaat gagcatgggt cccgctagct
     2821 ggtatcccaa atatcttccc agcacgtaca ttgcttgttg catctgctcc gccgatatat
     2881 gccgctctag ttccccagac tgctgcatcc agttcttgtg cccgtctagt tccaaattcc
     2941 aatagtggat cttctccgat gacagatttg atacgagctg cttttgtcgc gatcaacgtt
     3001 tggaaattga ccatattcaa cagtgctgtt tctactaatt ggcagtgcgc taaaggccct
     3061 tccacttgga ttaatggttc atttgcaaaa actagttctc cttcacgagc agaacgaact
     3121 gttgctttaa attcaaaatt ttgtaaatac tccaaaaaac cttcaggata gacttctaat
     3181 ccacgcaagt aagcaatatc ggaatcattg aacgtcagat tctctaaata attgactaac
     3241 cgttccaatc ctgcaaaaac cgcgtatccg tggttaaaag gcatttctcg aaagtaacat
     3301 tcaaaaacag catgtaaatc agctcgacct aattcccagt atgtctgcat catattgatt
     3361 tgatatagat ccgtatgcag cgtcaaactg tcgtctgggt aagtattatt catcttgtat
     3421 tactcctctt ggcaagtttt catttcctac ccattataac aaaaaaaatt aataggaaaa
     3481 gaaatcttcc attttttctg aaaaagcaca tgttttacgg atatttcttg ctaaaaatga
     3541 actccatctg ctaaaactat cctttcttat tggtatttaa cctaaacgag aaagaatact
     3601 tattttattc aatgtatccg agacacgaag aaattagcgt tttctatttt tcttacttga
     3661 taaaataagt agattttcct actacaatcg tagccgttaa gataccgatg attgaacaaa
     3721 tataaccaaa tgtctctaca acataatcaa tggcaaaagt ttttgaagca ataatacgtc
     3781 tttccttttt tgcttcttat ccccaaagaa acggatataa cttattatgt attgaatttt
     3841 tttggaaagg cgaattatga caagatatac cttgcgttga aaaaactcgc ggaaaaaata
     3901 tcacacgttt ttaaaaagat acaaagtaag aaagcagctg tcaatagaat ggaaacaaaa
     3961 atggcgagac atcaatcagt ctcgccttgt tttttattgt atacattaga tatggagcag
     4021 gttaccataa agatgattat tagtcgttat taaatagaat ccttcgtatt tttttgttct
     4081 atcactacaa tatctttatt tggacgtttg aattgtattc cggcacgttc tagctcctgt
     4141 ttcactccta catggacttt gacatcttct cgtgtcattt ttaaaacgtg atgaatcgca
     4201 tagtcgattt cttgccttgt gatttttttc ttcgtattga tcatcacgac ttgcttttta
     4261 tcatatgtgt ctaccaatag cagttgtgtt cgatctaacg aaaataattt gtaataatta
     4321 atagccgtat tcccaaaaaa gaattccact actttaggat aaaatgtctt aatatttaaa
     4381 ttactattca caggtgtttc gattagcttc atctcattcc tcttttctat atagtcgatt
     4441 tagatccaat cttaagttat gttaccatct ttcctagcat taaggaatcg gttacaattg
     4501 aacctttaaa gacaaaagga tttacctttt atcacttaat agtataacat aatttcgtga
     4561 gaaaaaaata caattttttc ttgaccatct cgttacgtaa aggattatgc ttacatttgt
     4621 caggaggaaa aataatggag tacacaataa agaaaatgtc agagatatca ggagtcagtc
     4681 cgagaacctt gcgcttttat gatgagattg gactgctaaa accagctagg atcaattctt
     4741 caggttatcg gatttatggc aaaaaagaag tcgatcgtct gcaacacatc ttattttatc
     4801 gaacaatggc attcaagttg gatgatatcc aagaggtctt agacaatcca tcttttgacc
     4861 accagaaagc tttaatcaag catcagcaaa tgttgctgga aaaacgggca caaatcgata
     4921 ccctcttgac aactgtccaa cagacacttg atatgtacga aggaggaaga aaaatgagtg
     4981 acaaagaaaa atttgaaggg tttaaacaac aaaagttgaa agaaaatgaa gaatcctatg
     5041 gacaagagat tcgacaaaaa tacggaaaaa aaaaggttac agcatccaat gaaaaatgga
     5101 tgaatatgga taaagaaacg tatgaagaaa tgcaaactgt tgaaaagcag atgttatttg
     5161 atctcactct ttacttaaaa gaaccagaag atgaaagctt agcagaccgt atcttccaag
     5221 ctcataagaa gtggttctct tacagctggc ctgaatatca gccaaaagcg cataaaggac
     5281 taggaatgat gtacgaagcc gatgaacggt tcactgcata ttatgacgag cgaagtggtc
     5341 atggtgcagc aaaagcttta aatgaaatta tccaaaaaaa ggcgtaatct tttacatgat
     5401 ccaacaaaaa agtacacgta aaatgaccta ataacaaaac agcagacacc caagttagat
     5461 ttttatgcta acttttggtg tctgctccat tctttcacaa gttttttcat cctactttaa
     5521 atatattcat cgttgaataa acgtataagg tattttcact ttatcctttt tttctgtaag
     5581 aaacaattga aatgccattt ctccgcaagc ttccagatgg tggtcgatgg ttgggaaacc
     5641 aagaagctcg ctgatcagca aattatcgcg tccaatcacc actggtgtct tatcttgcag
     5701 atagctttgc aagatgcctg ctgcaaccat atccgaattt gtaaagacac catcaactcc
     5761 aagctcttta aaaaagaagc ctgcttgttt tcctttctct gcatctatac aatcccaaaa
     5821 aacaagtgat tcatcaaaag atggaaaaag ttgatgacat agctgcaaag tgatttttga
     5881 gttataactt agcctgccac ttcgccccaa cgtgatgccc aattgcttta taccttgctg
     5941 cttcaaataa gtaagacctt ctgtaattga ttgttctcga tcaataaaga cacagcttgc
     6001 ctgttttccc ttgatttctt cacaaaaaac tagaggtcca tatttttgat atgccaataa
     6061 atcatctagt gtattggcac gagacgtaat gatcaatcca tcgaaagctt tagctgcaaa
     6121 ttcttcaaga taccttcgct ctttttcttt atgatagccc gtcggtaaaa gtgtcacttt
     6181 gtaatcataa tcaaacgctg cttcaatgat cccactgatc aactgatcaa aatacggatg
     6241 attggtataa ggcagaatca ctccaatgct tttcgttttt ccataactta actgtctggc
     6301 atttgcatta gggatataat cgacttcttt catgaccgca agtatttctt tccttttttc
     6361 ctcatctaca tatggatgtc cattgattac acgtgaaata gtagaaactg aataacctgt
     6421 gagctttgcg atatctcgaa tgtttgccac tgctgcttcg ccacctatac aaaaagattt
     6481 tcttgtaaat agtctaccac agctggaccg cattcaaaaa actgatacat ttttccttca
     6541 tgcgaaaaac tgatccgctg cccgccccaa tatatatctt ttgtcaaatt ttcgatttca
     6601 ttcattggta tttcttttac tatattccta ttcaaccctt gaaaactgta caacgttaat
     6661 acggatccct tgaactcgac aaacgcataa aaataattgt tcgttgtgcc atctgttatc
     6721 tctacataag tattcattgc aagccctcct tttgatactt tgagtatagt gtatgaaacg
     6781 cgtctcatag caaggagttt gttttgccaa agaaaagttt tattcctctt tcattattac
     6841 caaagtctct tattttaaat tgaatacatt ataatgatac tgtaaatata tctttattat
     6901 tagtgaatg
//
LOCUS       DACEXQ010000140         6906 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.140, whole genome shotgun sequence.
ACCESSION   DACEXQ010000140 DACEXQ010000000
VERSION     DACEXQ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6906
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.140"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IXH04_001975"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IXH04_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAQ1852567.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="IXH04_001976"
     CDS             complement(322..891)
                     /locus_tag="IXH04_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAQ1852568.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="IXH04_001977"
     CDS             1077..1979
                     /locus_tag="IXH04_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1852569.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IXH04_001978"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IXH04_001978"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAQ1852570.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IXH04_001979"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IXH04_001979"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAQ1852571.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..>6906)
                     /gene="dnaE"
                     /locus_tag="IXH04_001980"
     CDS             complement(5002..>6906)
                     /gene="dnaE"
                     /locus_tag="IXH04_001980"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAQ1852572.1"
                     /translation="FKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGSDEAFLT
                     QYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAKTLLLFQ
                     RGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARKKGKEAI
                     RYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKDVLDEEK
                     SHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKAHHPGAF
                     FTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFGLTAIKG
                     IRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELSPNRKQL
                     MLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLSGHPAEG
                     FDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSGEISVTV
                     FPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPSQTCYLR
                     ITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSIKGQLIY
                     LLGEDNVVFK"
BASE COUNT         1945 a         1502 c         1202 g         2257 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctggtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaa
//
LOCUS       DACEXQ010000141         6890 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.141, whole genome shotgun sequence.
ACCESSION   DACEXQ010000141 DACEXQ010000000
VERSION     DACEXQ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.141"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..2602)
                     /locus_tag="IXH04_001981"
     CDS             complement(<1..2602)
                     /locus_tag="IXH04_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1852573.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRS"
     gene            complement(3030..4103)
                     /locus_tag="IXH04_001982"
     CDS             complement(3030..4103)
                     /locus_tag="IXH04_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1852574.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(4100..4927)
                     /locus_tag="IXH04_001983"
     CDS             complement(4100..4927)
                     /locus_tag="IXH04_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1852575.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(4928..5734)
                     /locus_tag="IXH04_001984"
     CDS             complement(4928..5734)
                     /locus_tag="IXH04_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1852576.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(5746..6831)
                     /locus_tag="IXH04_001985"
     CDS             complement(5746..6831)
                     /locus_tag="IXH04_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1852577.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
BASE COUNT         2108 a         1463 c         1126 g         2193 t
ORIGIN      
        1 tacttctaga aacataataa aattcatctt catctggttt caaatcataa ttcggttctt
       61 cagatttttc tgtccgagcc aagaataaac gttccatcat caatgccgta tgcatataga
      121 gatttagttt tacacggcca tttaaagtga aatgatagta attttcatat ttagaaatga
      181 ctgtttccac ttcactcatc acgatcgttg ggttcagaaa gttcaaacgg tccgcaattc
      241 cttccaacga aaagaatttt actagctcct gaatcaagcg gttaaattct gccggcttca
      301 tttcaggact caaaatatgc tccaaattgg ctgctccatc accgtctaag atgtcataaa
      361 cattaatatt aggaattgaa aaggaacgtg gaagatccga agtcgtgatg accaattttg
      421 tcttgtcaaa ataagtttca tcattttggt cgattaattc acgtagttct ctgtagtcct
      481 tcgtgaaaac ttcgatggat tcatccgata aatgatgacg aaaaacatct ttcaatttgt
      541 ccgaaatccc caaacccgac atacaggaaa tgataatgtt gctactttga gaaaagcctt
      601 caaagtattg aacattgatt gaatacttgt ctgctgcatc ctccgcaatc tgtttaaatg
      661 gcgcattcat cgtcatcttc aagccaatat caagtgcaat ggaagtcgtc aaattattca
      721 gaagaagtaa ctcccctttt aaatcgttct tgatctgtgt gtaaatttga tttaaagagc
      781 ccatatctac gatcagcact agactatctg tattcacttg ccgtttcacg aaattccttg
      841 tcgattcgac aatctctgac acattactct ctactggcat atccaacgct tcaaaaacat
      901 aattgccgca taattgattc actactgctt gaatgctgct agccgtacta tctccgtgag
      961 caagcaacag gcccttcagc tcgatggatt catctacata atcagataaa gtcacgacaa
     1021 taagcagtct tagaaagttc tcgtcctttt ctgagagcgg tggtaaatgc tccaagaact
     1081 gattacataa gtaagtcgtc cgaggtagat ttgcagctaa tgtacgattt atcccggtta
     1141 agtctgtctt atagcttggg gaaatgacat aggatctgct aataatttct gcaacaggtc
     1201 cactcgtcgt caatccgtac gtttttccta aaacggatac ccagaccttt ctaaacatct
     1261 gaatcatcaa ttttctactc attgataaaa aagaatcctt atcaatgtga tgcaccaaat
     1321 gctgtacttc attctttatc gaagaaaaat tttcattgaa attaccttca tttatcagct
     1381 gagtaatttt ttccaaaatc gaatcataat caaacggttt gattcgatcc gtttttatct
     1441 tttgattttt ctttatcaat aaactagcaa tcggcaagct ttctacttcg gattccagca
     1501 tatttaactg aatcaagatc tcatttttcc caatcgttaa tggttcttct tgtgttcgac
     1561 tatacacatc cgcacaactg attttaatga tattcttcag cttaccaata ttcccactaa
     1621 agggatgaga taacattaaa gacaaggctt ctcttgaaac aataatatct ttttgtaaaa
     1681 catccgcttc attttgaaaa aataattgaa tcagctctaa tcgttctcga atggggcgat
     1741 cttcaaaagt tggcaacata acactaattt gaatacgccg atcaaaggtg tctaataaat
     1801 aattttcacc tttttccgtc gtggcgaaaa taaaacgaac cttagcagaa tgccaattta
     1861 cattttcacc gactggacga tagtttccag tatcaataaa tagaaagagc ttttcttgat
     1921 tttctttcga taggcgatgg acctcatcga gaaaaagata gccgttgttg gccgttgcca
     1981 ataaaccatc ggtatccttt tcagcccccg taaaggcccc cttcttgtat ccaaacagtg
     2041 ttgccgaaag cagttcggga ttgtccgcat aatcggcaca gtttaagata gcaaatggtg
     2101 catctttttc aataatttgc tcatggatag cgtattcaaa aatttttgtg gctaaaaaac
     2161 tttttccaac acccgtggct ccatttatta atacactgag tccgttaggc ggatatttga
     2221 cagcggctat acatttttga atgacctctc tcagacttcc gtcatgacca actacagaat
     2281 gaaaggcttc gtttatttgc tccgtattat ctacaaccgc atattcatct aaaggcttcc
     2341 acaaaacagg ctttcccggt aatttctcga cctttttatc ttccactaat ctcgtcaaat
     2401 aatggctggc attctgacga gagataccta gattcttcga aattttctcg gcagtgacag
     2461 aagacatatt ccctgttagt cgtatcagct catcaaaaac tcgatcttta gcgctctttt
     2521 tcattttttc ctccagttta atgtgtctaa aagcataata aactatcggc tgcaatatgt
     2581 ctattctata tttgtattcc aatttttact ttgaaacaca aatagtcgaa tatttgcctc
     2641 agatatctga caaaataatt tatctaccaa tttagttttt atctaatatg atttttatgt
     2701 tttttcatag ctacaaatat tgaactggat tcatatatta gttttattaa tagattacag
     2761 aagggttaag tatatttgca tagtttccaa ttactttatc cttctgtaac tttttcaatc
     2821 ttagatttac tagcccgtct cgggccctat tttacaatgg ttttgtccac ttttctctgg
     2881 tagaaaaacg tggtagaaaa aataaaataa cacaaaaaaa gcggcgtttc atccgctttt
     2941 ggtagaagat tggaaaactc agatagaaaa aggacgattt ttagctcttt agaacgttga
     3001 taagtcccca ttcttactga gttttgccat tatctcctat acatcttgaa ttctaagaat
     3061 aggtcattat agatgcctag gtatttcgat cctagatctt tgtagacttc aagatgggag
     3121 atcgtgtcat cagatggata gaattgttcg tccgacgaaa tgtcttttgg caataacgct
     3181 tttgcttctt catttggtgt agaataacca atatactctg cattttgtgc cgcattttcc
     3241 ggttctagca taaagttgat gaaatcatag gcaccttctt tatttttcac ggtttttggc
     3301 ataacgatat tatcaaacca aagattggat ccttctggtg ggatcacata atgtaaatgt
     3361 tcattttccc acatcatatc ggcagcttcg ccagaaaatg tcactgccac ggaggcttct
     3421 tcattgatca tatacatttt gatctcatct gccacgattg ctttgacatt tggtgttaaa
     3481 tgattcaatt tatttgctgc ttctgtcagc tgtgccatat tcttactatt caaagaatag
     3541 cctaaactat tcaaagaaag ccccataact tctcgtgcac cgtcaatcag catcaaactg
     3601 tcttttaatt ccggcttcca caaatcatcc caatgcttaa tttgatttgc atcaaagaat
     3661 ttatcgttat atacgatccc taaagttccc caaaaatatg gaatcgaata ctgattatct
     3721 ggatcaaaat ccaagtctaa aaatcgtgga tcaatattat ttaatccttt gatcttcgaa
     3781 tgatctaatg gaagtactag tttttctttg atcatttttt ggatcatata ttcacttggt
     3841 atagcgatgt cgtagtttgt cccaccttgc tggatttttg tgaacatcgc ttcattagaa
     3901 tcaaatgttt cataattcac tttgtatcca tattcttttt caaatttcgt gatcaaacta
     3961 ggatcgatat aatctcccca gttatagatg gtaagtacct ttgctccact catgccactt
     4021 gttttttcaa gttgacgaac accaagcagc agcaccgcaa taatgatgag aatacctaaa
     4081 aacagagatt gtaatttttt catcgcagcc gtgccacccc ctcgcgtact cttctgtttt
     4141 tcttcagccg ttttggacgg ttttctttac taataaagta gtatccaatg accaatatca
     4201 tggagaacaa gaaaactaac gcacttagcg cattgatttc taaactgatt ccttgtcttg
     4261 cacgagaata gatctctact gagagcgtcg taaagccatt tcctgtgacg aaaaatgtaa
     4321 ccgcaaaatc atcaagagaa taggtaaatg ccataaaata tccagctatg attcctggag
     4381 aaagataagg caaaatgata tttttgacta cttggaaatt attagcacca aggtctctag
     4441 ccgcatcgac cattgaatcg ttcatttcct gcaattttgg caagaccatc aagacgacga
     4501 ttggaatgct gaaagcaata tgggaaagca atacactctg aaaacctaat ccgaatccta
     4561 gtagtgtaaa gaaaatcaag aaactagcac cgataataac atcaggagag actaataaga
     4621 tgttattcat gctcaacaac gctgtccgtg cacgtcgttt tttcgtgtaa tagattccca
     4681 tagccccaaa tgtcccgatg atcgtggcga tcaatgcaga taagaaagcc agcatgaacg
     4741 tattcaatac gataatgatc aaacgtgtat cttcaaaaac agcttcataa ttttcccaag
     4801 taaatcctgt aaagctgttc atcgttcctc cttcattgaa ggaataaaaa atcagataga
     4861 agatcggtgc atataagaat aaaaagacga tccctaaata aagatacgac catttaaatt
     4921 ttttcattta tgagcgacct ccttttttct tctcgcctgt caggagcatc acgatgaaca
     4981 tagcaatgat taaaatcaca ccgattgtag aacccatccc ccagttttgt gtaaccaaga
     5041 aatgctgttc gatagccgtt cctaaagtga tcacccgatt tccgccaatc agacgagtca
     5101 gcatgaataa agaaagagaa ggaataaata ctgcttggac accacttttc acaccattta
     5161 atgataacgg aaaaatcact cgtcggaagg tctcaaagct attcgcgcct aaatctctac
     5221 tagcactgat caaagaagga ttcatctctt ccaatgcatt gaaaattggc ataatcataa
     5281 atggaatctc gatatacgtt gccacgaaca gaaaactgaa gtctgtgaat aatatctgtt
     5341 tcggcccgat cccgataaaa tcaaagaaat gattcacgct gccgtgcaca ctgaaaatcc
     5401 caataaatgc gtaagcttta agcagcagat tcacccatgt aggcaaaata atcagcatca
     5461 gccataattg cttgtgcttc agttttgtta aaaaataagc agtcgggtaa ctgatgatca
     5521 gcgtgaaaaa cgtgatcaaa aatgcatacc atacagaatt gatcgtcata gaaagcgatg
     5581 tccccgacgt aaaataagat agataattat tcagcgtgaa ttgcccattc atatcaaaaa
     5641 aagattgata aatgatcatt aatacaggcg cgatcacgaa taaaccgagc cacaaaatat
     5701 aagggacgga atagatcctg cgcattgttt tcattcttgt cccccctatt cttcgtagct
     5761 gtcaagacgt gcatcgaatt cttcttcaga ctcgttgaaa cgcatgacat ggatatcttc
     5821 tggctcaaag gccaatccta ctttgcttcc ttctttagct tttttcgttg aatggaccat
     5881 ccattcattt tgctgttcat cataacagat gatttcataa tggacaccac gaaacagctg
     5941 agtatcgacc gttacgacta gttttccttt atctggtgtc gtgatcgtca aatcctctgg
     6001 gcgtaaaaca acttcaactg gttcatttag acgcatcccg ccatcgacac attcaaattg
     6061 cttaccaaca aactcaacta gattatcttc gatcattaca ccgtccacga tattgctctc
     6121 cccaacaaaa tcagcaacga aatgattgat tggctcatca taaatatcta cgggtgtacc
     6181 actttggacg attttccctt tattcatgac aaaaatctca tcactcattg ccaacgcttc
     6241 ttcttggtca tgagtaacaa aaataaacgt gattcctaat cgttgttgca gatcccgcag
     6301 ttcatactgc atatctgtac gtagtttcaa atcaagcgcc gataacggtt catctaatag
     6361 caatactttc ggttcattta caattgctcg tgcaatcgct acacgctgtc tttgaccacc
     6421 agacatttca ctaatctccc gtttttcgta tccaggaagc tggaccatac gcaaagcatc
     6481 ttttaccttc ttttcgatat ctgatttgga cattttcttg atcttcaatc caaaagcaac
     6541 gttatcaaaa acattcatat gaggaaaaag tgcataatct tggaagactg tatttacttg
     6601 tcgcttatta gctggcaaat cattcaaccg tttcccttca aaatagatgt ccccttctgt
     6661 agcttcggtg aatccagcta taatacgtag gatcgttgtt ttaccacagc cggaaggacc
     6721 aagcagtgta taaaattttc cttgttcgat ctcaaaactt acttttttta atactgtttc
     6781 atcatcatag cgtttcacta cttgatcaaa tgaaatgatt ggtttcccca cgtttttacc
     6841 ccctatatta caaatatgat tgcgttgcaa caatcaatac ttttgttcgt
//
LOCUS       DACEXQ010000142         6841 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.142, whole genome shotgun sequence.
ACCESSION   DACEXQ010000142 DACEXQ010000000
VERSION     DACEXQ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6841
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.142"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..882
                     /locus_tag="IXH04_001986"
     CDS             <1..882
                     /locus_tag="IXH04_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAQ1852578.1"
                     /translation="ISVPVALFSFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTY
                     NDYFQIGMIGMIVSLVVVLTVANISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVL
                     GVVLLDLQIILGFILAGIWALLFTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMT
                     LAMFNNSAAYASPYFSAIFGDWIPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILA
                     VVLAVNPTMSPAFLFPLFAITTIAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGW
                     IIGAILCLITFLLYGNA"
     gene            930..3059
                     /locus_tag="IXH04_001987"
     CDS             930..3059
                     /locus_tag="IXH04_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAQ1852579.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKNLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKAIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPHTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3034..3723
                     /locus_tag="IXH04_001988"
     CDS             3034..3723
                     /locus_tag="IXH04_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852580.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKEIE
                     KRCTEYDLDYK"
     gene            complement(4131..4817)
                     /locus_tag="IXH04_001989"
     CDS             complement(4131..4817)
                     /locus_tag="IXH04_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1852581.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(4953..5147)
                     /locus_tag="IXH04_001990"
     CDS             complement(4953..5147)
                     /locus_tag="IXH04_001990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852582.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5197..5637)
                     /locus_tag="IXH04_001991"
     CDS             complement(5197..5637)
                     /locus_tag="IXH04_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAQ1852583.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELEQLFQAYASLDDVNS"
     gene            complement(5641..6486)
                     /locus_tag="IXH04_001992"
     CDS             complement(5641..6486)
                     /locus_tag="IXH04_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAQ1852584.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVDWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLVANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAVYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            6635..>6841
                     /locus_tag="IXH04_001993"
     CDS             6635..>6841
                     /locus_tag="IXH04_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="divalent metal ion-dependent extracellular
                     dipeptidase; able to hydrolyze a broad range of dipeptides
                     but no tri-, tetra-, or larger oligopeptides; differences
                     in the amino acid specificity of the cleavage site varies
                     between species; similar to succinyl-diaminopimelate
                     desuccinylases; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAQ1852585.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNY"
BASE COUNT         2081 a         1294 c         1419 g         2047 t
ORIGIN      
        1 atttcagtac cggttgcact gttctcattt atgggctcga ttatggctgg aatctttgct
       61 aatatcgtca atttcaaaca atatcaaacg atttatgctg gtttcaatcc agcggctgaa
      121 agctatactt ataatgacta tttccaaatt ggtatgattg ggatgattgt aagcttagtt
      181 gtggtactaa cggtcgctaa tatatcaatg aacaaaaaga aacgctatgc aatggcagca
      241 aacgtaccag ctgaaggtgg cgacgcacca atgatctcat ggttagccgt attattacca
      301 gttttagggg tcgttctttt agatcttcaa atcatcttag gttttattct agcaggtatc
      361 tgggcattgc tgtttactgg aaaattgcgc ggaggttata aagaaatctg tcgtcagttt
      421 gcgaaattat ttacagatgg cgcagtcgat gttgcaccaa tggttggttt cttgatgacc
      481 ttagcgatgt tcaataattc agctgcgtat gcttctcctt atttttcagc tatttttgga
      541 gattggatac cgcaatcacc attagtcttg gcaatcgtat ttgctatatt gacaccacta
      601 ggatttttcc gcggtccaat gaatctagtc ggttcaggct cagctatttt agctgtcgtg
      661 ttagctgtta atccaacgat gtcgccagcc tttttgttcc cgttatttgc aattacaacg
      721 attgctcctc agcatttaga tattacacaa tcttgggttg cttggggatt aggttataca
      781 aaagtaactt ctcgagaata tatgaagaaa tccatcccaa ctggttggat catcggtgcg
      841 atcttatgcc tgatcacgtt cttattgtac gggaacgcct agtaaacaaa acgtcgataa
      901 aaacgattag ataaatggag gaaaagaaaa tgaaacgttt tgtgcgaatg ggaattgatg
      961 ttggcggtac acatacgaaa gcagtcgcaa tcgataatgc gacgcacgaa atcatcggaa
     1021 aatcttcagt gaaaacaacc cacgatgatg tccgtggagt agctgcaggt gtcgttcagt
     1081 cttttcaaaa ttgtttgcgg gaaaataata tcagtccaga agatgttgtg tttgttgcac
     1141 attctaccac acaagcgact aatgcactga ttgaaggaga cgtagcaaaa gtcggcgtaa
     1201 tcgggatggc aaaaggtgga ttagaaggtt ttttagcaaa acgacaaact agattgaacg
     1261 atatcgattt agggaataag aaaaaaatag agattgtcaa tgcattctta cctgtcaaaa
     1321 acttaaatgt agaccgtgtt tcagaaacga tttcttcttt agaaagagaa agagcggagg
     1381 tcttggtttc ttctatggca tttggagtag ataatgggga accagaacga gttgtttatg
     1441 aagcagcatc tgtgaaagcg atccctacaa cgatggcttc tgacatcaca aaattatatg
     1501 gattgacaag aagaactcgg acagcagcaa tcaacgcatc tattttacca aaaatgctag
     1561 atacggcaac ctcgacagaa gactccgtac gagaagcagg ggtcaatgtt tctttgatga
     1621 ttatgcgcgg agacggcgga gtaatggaaa tcaatgaaat gaaaaaacga cctgttttaa
     1681 ccatgctttc cggtcctgct gcatcagtca tgggctcttt gatgtacttg agagcgtcta
     1741 atggtgttta ttttgaagta ggcggaacaa caaccaatat cggggtcatt aaaaatggac
     1801 gtccagcaat cgattattca attgtaggtg gccacccaac atatatctct tcattagatg
     1861 ttcgggttct aggtgtggca ggcggttcga tggtacgggc taatcagtca ggaatcatcg
     1921 atgtcggtcc acgttctgca catattgccg gtctagatta tgcagtattt acggagacag
     1981 aaaaaatcaa aggtcctaag gttgaatttt tctctcctaa agaaggagac cctgctgatt
     2041 acgtcaaggt agtgatggaa gacggagaag aagtgactat tacaaataca tgtgcagcca
     2101 atgttcttgg attagtgcaa gaagaacatt tttcttatgg aaatgtccca tctgcacgaa
     2161 aagccataca ggcattagca gactattgtc atacgacggt tgaagacatc gcagagcaga
     2221 tcatggaaaa atcctatgct aagattgaac cggtgatttt agaattggca gacaagtatc
     2281 acttggagaa agatcagatt tctcttgtag gtgtaggggg cggcgctgcc tcattgatta
     2341 cttactttag taataaaatg ggtgtgaaat attcgattcc tgaaaacgcc gaagtgattt
     2401 cttctattgg tgtagcactt gcaatggtcc gagatgttgt agaacgtatc atcccttcgc
     2461 caagtaaaga agatatacga tcgttgaaaa atgaagcgat gaataaagcg attgaatcag
     2521 gcgctacacc agaatcgatc gaagtccacg tagaaatcga tccacatact tcaaaagtaa
     2581 cggctatcgc tactggttct acggaagtga aagcaaccga tctaaccaag gagatcacaa
     2641 cagaagaagc actagagctt gctgcagagg atatgcgatt aaataaaaat gaagtttgct
     2701 tattggaaaa tacacctttc ttttatgtat gcggcgaaca gaatcgttca aaaaatgctg
     2761 gaagtcttcg gatcatcgat caaaaaggat ttatcaaggt ccaacgtgga catgcttcat
     2821 gtatgaaaac aactgcagcc aattatatga cagctgtcga acaactttgg gaagatatgg
     2881 cagtttatca aactgaattg attgcccgac cagaatttta tctctgctta ggagcaagag
     2941 tctctgattt tacagcgacg gatctggaac aactacagct tttgatggat ctggaagtat
     3001 ctactatgga accggaggaa gaagtgatcg tcgttgccgg aaatatcaaa caaacataag
     3061 ttagaagagt tactaaacga attacaacag atccccgaag aaatttgggg attctaccag
     3121 tttcggcgag atttattttg gaaaaagatt cctttgtcca agcagaaaat tcttatacaa
     3181 caatcaatcg attgtggtat cgaaacggca tgtagcatca aaaaaaagta cccttttgcc
     3241 gatgtaggag aaatctgtga acaaatggct gttccaatcg tttcctgtga atcagagcaa
     3301 ataaacgaac gaatcacttt tgcaacttat gcagaagatg aagggatccg attgatgacg
     3361 gagccgttag aaaaattaaa atgttcagga ctagcttcga tctctaaaga aacagcgcaa
     3421 gctttgatta ttggacatga actatttcac cacatcgaag cttctgttaa gggaatttat
     3481 acgcaaagcg aaaaaattgt gctgtggcgg ttgccttttt atacacatca atcaaacatt
     3541 cgtgcattga gcgagatagc agcgatgagt ttttcaaaag agatgaatca aagcgatttt
     3601 tcgccttatg tattagaggc agtcttactt tggccatata atgaaattcg tagccaaggg
     3661 atcttagaag aaataaaaga aatagaaaaa agatgtacgg agtatgattt agattacaaa
     3721 taaagagact gtgacaaaag tcgtgtcaca gtcctttttt tccgaataaa cggtgttctc
     3781 ctgtcaactc ctcggtcaca agtcaaaata ttcaacaaac atgagaagct gttttttgaa
     3841 attgttcctt gtgactcgga gatgaacggc aggctcacga cctcttggaa aaaggtgttc
     3901 aaaagcttgc atctgcggta ataagaacag ccaagttaaa aacataagaa gctattttta
     3961 cttggctgtt cttattactt gatgcagggc agctttttca caacctctca ataaacggtg
     4021 tgcaatcagc tgaccctcgg tattagctca aaaaaaacgg caaaatatga ggaactattt
     4081 tgccgtttat ttctctaata ctcaggtcaa aacgctgatt ccaaaacctc ttatttgatg
     4141 ggtctgtcct gaatatcttc aaaataatcg gggtaattat gaatcacgta ttcttgctcc
     4201 tcgataatca tatgtaaatg catcgtgcaa agaaatccga cttcatcaaa gttatgatca
     4261 atcattgatt gaagcattgt ctgatgttca tctaatactc gaccccagtc taattctgaa
     4321 atcgtcaaac gtaaccatct gaaacggttc aagtgcgtac tataactttc tagccaatcc
     4381 caaatttctc gttttccagc aatttcataa caagttcggt gaaaatcagt atccaaatca
     4441 aaaaaagctt ttttattttg agcgatcact gcctgatcgg tttgttctag tattttttct
     4501 agtttagctt gatcttgctt ggtcattttc gcactgcatt cttgcaggat cggtttttcc
     4561 aacttttccc gcgtataacg ggctaatgaa gcagatcgga tatcgatttt tgaaacataa
     4621 gtgccggact gtggaatggt ttttacaagc tcttgattgc gcagttggat cagtgcttct
     4681 cgaatcggtg tacgcccgat agctagttgt tcttctaaca ctttatcgga gattttactt
     4741 ccgggtgcta actcggaata aatgatttgt ttacgtatag attcataagc ttgctgttgc
     4801 aaattattga taaccattta accttccttt ctatgcagat ataatctatt ataacataaa
     4861 tcgcttacaa atcgtgtggg cataaaaaca cagtctgcaa aaaaagccct gataggcatg
     4921 cctattagag cctcgaaaag aaaaatattg aatcaatctg gtcgtacctc ttggatcggt
     4981 ttgacacgac cgccgattct tttagcaaaa gcttcggatt cttttttgtc tttgaaaatg
     5041 agcagattgt ctaagttttt ttcccgagtg cgacagccag ttttatcgat gtaaccgtgc
     5101 attactttaa cgacatacat ggggatcacc tgcttttttc ttttcattat acgaactact
     5161 cgacacaata caagaaaaat gcttagacgg tctttattaa gaatttacat catccaaaga
     5221 agcgtacgct tggaataact gttctaactc cggattgact ttgctcagct tcactaatcg
     5281 ttgcgaatca gaaagtaaaa aacagtgttt actactgcca gtagttttta attttttgtc
     5341 ttcagttccc acaatttcgt agctaaaatc caaccgtgtt cccgtatact tttggatagt
     5401 tgggtggatg ctcacttttt ctccgtaatg gatcatttct ttatagctgc aatttacttc
     5461 cagaacgggg atgatgatcc cagattcttc gattttttta tagggaaacc ctagatgtac
     5521 caataaggct acccgagcct cttcaaacca gcggatataa ttgctgtgat gaataatccc
     5581 catttgatct gtttcataat aaaaaggttc tcgcacataa cctgtaaatt tctccatgac
     5641 tcatgcctcg ttttccattg attttttaca aaaataattc atccacttcg gaagtgcttc
     5701 gctgatttgt gtcggcaaaa caatatattg ttcttttgcc agctggtctc caatgaaact
     5761 atgaagataa acagctgctg caatcgtttg gtcattttga ggaaactggg ctaaaaatcc
     5821 tgtgatcatc ccagctaaag tgtcacccat gccgcctgtt gccatagcag cagaacctaa
     5881 aggattggtg tagaagtgct ggtgaccata aatttttgtc tcatgacttt tcaaaacgag
     5941 cacagcaccg atttcctgtt gcttcttttt gttttcttta tcccgttgtt cttgtatcgg
     6001 tagagaactc aaccgttgcc actccatttg atgaggtgta agtacagttt gttcgggata
     6061 gggtagcaaa agatgattag caacaagtaa acttaaagca gagccatcaa tcaccagcca
     6121 ttgttctttt gattggtttt ctagtacttg tttgaaacgt tccaaactct tagaagaagt
     6181 acccaatccg ggaccaatca agagtacatc tgcttgcttt attacggaaa gaaactggtc
     6241 agtatccgtc caatctacaa tcattgcttc tggcagatga ctgtgaagag cactccgatt
     6301 tttttcatca gttactacag tcgtgagacc cgctccactg tgtacggcag ctaaagcact
     6361 cataatgatt gctccgccat attgttcatt gccgccaatc aaaacgatac gtccaaatgt
     6421 tcctttatgt gaaatctttg gacgaggtgt gatgactctt ttcactagtt gttcgtttat
     6481 cagcatcgaa aaacctcctt ttactcatta tagcgtatat tttttttaga gaaaaacaga
     6541 taagaaaagg aattgtcaga ttttttgaac tatttttttc tgtaatttgt ggtatcatga
     6601 acacgaagac ttaaataagt ggaggattaa ctttatgaca attgattggc aaaaagaagt
     6661 agaagcacgt aaagaagact tgcttgaaga cttgaaaaat cttttacgtg tcaacagcga
     6721 gcgcgatgat tcaaaagtaa caccagatgc accatttgga cctggacctc gcgacgcatt
     6781 gaaacatatg ttagcttatg gcgaacgtga tggatttaaa gtaaaaaatg tcgataacta
     6841 t
//
LOCUS       DACEXQ010000143         6794 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.143, whole genome shotgun sequence.
ACCESSION   DACEXQ010000143 DACEXQ010000000
VERSION     DACEXQ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6794
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.143"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(203..661)
                     /locus_tag="IXH04_001994"
     CDS             complement(203..661)
                     /locus_tag="IXH04_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ1852586.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(674..1999)
                     /locus_tag="IXH04_001995"
     CDS             complement(674..1999)
                     /locus_tag="IXH04_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAQ1852587.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(2016..3428)
                     /locus_tag="IXH04_001996"
     CDS             complement(2016..3428)
                     /locus_tag="IXH04_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1852588.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(3445..4209)
                     /locus_tag="IXH04_001997"
     CDS             complement(3445..4209)
                     /locus_tag="IXH04_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAQ1852589.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4313..5188)
                     /locus_tag="IXH04_001998"
     CDS             complement(4313..5188)
                     /locus_tag="IXH04_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1852590.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5443..5527)
                     /locus_tag="IXH04_001999"
     tRNA            complement(5443..5527)
                     /locus_tag="IXH04_001999"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5491..5493),aa:Leu,seq:caa)
     gene            complement(5545..5616)
                     /locus_tag="IXH04_002000"
     tRNA            complement(5545..5616)
                     /locus_tag="IXH04_002000"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5582..5584),aa:Gln,seq:ttg)
     gene            complement(5626..5698)
                     /locus_tag="IXH04_002001"
     tRNA            complement(5626..5698)
                     /locus_tag="IXH04_002001"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5663..5665),aa:His,seq:gtg)
     gene            complement(5760..5844)
                     /locus_tag="IXH04_002002"
     tRNA            complement(5760..5844)
                     /locus_tag="IXH04_002002"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5808..5810),aa:Leu,seq:caa)
     gene            complement(5865..5935)
                     /locus_tag="IXH04_002003"
     tRNA            complement(5865..5935)
                     /locus_tag="IXH04_002003"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5901..5903),aa:Cys,seq:gca)
     gene            complement(5981..6052)
                     /locus_tag="IXH04_002004"
     tRNA            complement(5981..6052)
                     /locus_tag="IXH04_002004"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6018..6020),aa:Gln,seq:ttg)
     gene            complement(6064..6136)
                     /locus_tag="IXH04_002005"
     tRNA            complement(6064..6136)
                     /locus_tag="IXH04_002005"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6101..6103),aa:His,seq:gtg)
     gene            complement(6139..6212)
                     /locus_tag="IXH04_002006"
     tRNA            complement(6139..6212)
                     /locus_tag="IXH04_002006"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6178..6180),aa:Trp,seq:cca)
     gene            complement(6219..6299)
                     /locus_tag="IXH04_002007"
     tRNA            complement(6219..6299)
                     /locus_tag="IXH04_002007"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6263..6265),aa:Tyr,seq:gta)
     gene            complement(6301..6376)
                     /locus_tag="IXH04_002008"
     tRNA            complement(6301..6376)
                     /locus_tag="IXH04_002008"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6341..6343),aa:Phe,seq:gaa)
     gene            complement(6412..6484)
                     /locus_tag="IXH04_002009"
     tRNA            complement(6412..6484)
                     /locus_tag="IXH04_002009"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6449..6451),aa:Asp,seq:gtc)
     gene            complement(6494..6566)
                     /locus_tag="IXH04_002010"
     tRNA            complement(6494..6566)
                     /locus_tag="IXH04_002010"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6531..6533),aa:Val,seq:tac)
     gene            complement(6582..6653)
                     /locus_tag="IXH04_002011"
     tRNA            complement(6582..6653)
                     /locus_tag="IXH04_002011"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6618..6620),aa:Glu,seq:ttc)
     gene            complement(6668..6757)
                     /locus_tag="IXH04_002012"
     tRNA            complement(6668..6757)
                     /locus_tag="IXH04_002012"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6721..6723),aa:Ser,seq:gga)
BASE COUNT         2063 a         1486 c         1095 g         2150 t
ORIGIN      
        1 tatttcaata tattctctta aataatcaaa tttattttta agaatcctga aaataagttt
       61 tctaaatcat aggagcaaca aaaaaattcg ataattaaca aaaatcaaaa tgattaaatc
      121 tagtgattga aacatatcat ttcaaaaaaa ttttgtaaat tcacccataa atatcaccct
      181 atttaacttt tttcatcgta cactaaaatt caaaacaact atctttaatc tttacttctt
      241 ggttttgttt taataaattt ccttcaaaaa tgcctggaga aactacaatg atcaccgtat
      301 ctttttgtgc ttgctccaac ttcttcaaat ctataatagc taacagtgtc ccttctttta
      361 cctcctggtt actttcaacc cgtatctgaa acggacttcc ttcgagttca actgtatcta
      421 aacccatatg gatcagtatt tttctgccag attcactttc gatcgttatg gcatgtcggg
      481 taggaaatat attatcgatt attcctttaa ctggcgcatg gatatatcca tcatgattcg
      541 ttatagcgta tccaactcct atcatttttg aagaaaagac ctcgtctttt actgctgtta
      601 atggaataat ttgaccacta gctacagctt ttagaatgtt tcttgtttta aagaaatgca
      661 ctttttatca cctctacttt aatgctggcc aatatttttt gtttactttg atcatttcat
      721 ctagtatttt ttttgcgact ctggcatttg gtacggtttg attcaatgta aatgcttgta
      781 aagctttttg ataagaacct tcaaagtaag catctactag taacttctct actgcaactt
      841 gtgcctccat caatccttta tgaaaatcac tgattggttt tcttagtgta attggctcca
      901 caccagttgc accaacatat gccggtattt caacaactgc atctgaccgt agattcggaa
      961 tcgccccttg attaggaacg attagcataa agcgacgacg atcatcattc aacaaggaaa
     1021 tagctatatc gacgatatat tgtccatgct ccccaaaatt aaagttcaat acttcttcca
     1081 caataccgcg gcgtatttta tcagccattt ctttggtatt tttctctcgt ccatccatga
     1141 ccatattagc acgtgtatac tctttgtcag tatgctcaac gaccatatca ggatataagt
     1201 aatactctaa gtaattattc gggatattac ttggaaaatt tttcgtgatg actgacatca
     1261 tttgaaaggt tttttgccaa gttttatctc ctatattaaa atcggcgact tgcatttctt
     1321 ttgttattag tttttcaata atctctggca taatttctcg ttgcaattct ttatcataaa
     1381 tcgatgtata ccagccaaag tggtttaaac cataatacgt aacgatccaa ttcttacgat
     1441 cataaccgta attcactgca atcgtttctt caattgaaat agtcatatca caggcattaa
     1501 tcatttttac attcggatat ttacgacgaa cagcttcaga aacgatcgac tctggatttg
     1561 tataattcaa aatccatgct ttgggggcat agtcttgtat atgatcgaca atttccaata
     1621 aacctttgat tgagcgcatc ccataagcaa atcctccgag tccacacgtt tcttgtccta
     1681 ctaatccatg tttaagtgga atcttttcgt ccttttctcg catctccaat cctccaacac
     1741 gaatttgtga gaaaacaaaa tcgcatcctg taaaagcaag ctttggatca tccgttgcac
     1801 gaatgcgaat atttgaaaaa ccttttcttt tcagcataaa tttaacaatc aagtacatat
     1861 ccatatttct ttcttcatca atatcatata aacgtatttc agataatggc aaccgttttt
     1921 cattgttcaa aatggcttga acgattcctg gtgtatacgt actcccgcct ccagctatag
     1981 tgataatctg ttttttcatc atcatctttt cctttctatt cttctaataa tttattgaat
     2041 tctgccataa cataagtcac ttctaagccg atgataattt gataattttt ttcagttggt
     2101 ttaatgatcc cgcttatccc taattgcttg attcgctgtt cctcgaccat ggtgggatcc
     2161 actacctcta cccgtaggcg tgtattacaa ctatagacat ccaggatatt ggcttttcct
     2221 ccaaacgctt gtagcatttt tgttgccata tattcataat ttttacttgc taaccctaac
     2281 tctgtttctt ctaaggttac ttcattgcca aactctgcct tagcttcacg tcctaatgtc
     2341 tgaatgtttc tcttattgat caaataataa aaagtaacgt aatacaaggc aaaaaagaaa
     2401 attccgattg gaagaatcaa aatagcattg gtagcgattc gaaaattgat gagataatca
     2461 acaatacatg ctccgaatgt aaaaccaatt cgtatattga ataagtaaca gacaatccct
     2521 gctaaaccgg caaaaagtgc atggatcacg tacaacatag gagaaagaaa catgaaacta
     2581 aattcaacag gctcagtgat atttgcaata aatgaagttc ctgcaccgct tcccatgatt
     2641 cctttgacca tctctttatt ttctttataa gctgctttat agatagctag acatatagct
     2701 ggtataccaa acatcattac tacaaaaaaa actgataaaa acagtccagc ctgaggatct
     2761 ccgttgataa agcgtgtcat ctctcctctg aaaacttcct tgctaggaga tgtatagctt
     2821 cctaaatcgt aataaatata cgtattcaac acatggtgca gtccaaaggg gatcagcaaa
     2881 cgattcaaaa acataaatat ccccacacct aatgctccca tatcaacgag cagttttgca
     2941 aaattatcaa tcccaatttg gatgagcggc cagataaagc ccaaaaaaac agctgcgatt
     3001 gtttgtagaa tcatcaccat agtcaatggg aatttttcgc ccgcaaaata agaaaaaact
     3061 actggtaatt tttgttcttt aaagcgatta tacgtccatg ccgctactaa accggaaata
     3121 attccaccaa aaatccccat attcacttct gggtccataa tttctaagcc ttttttcaag
     3181 gtagcaatcg ctaaaaaacc agtaagtgca ggtattcctt tgtccttatt tttgacaaaa
     3241 ccgattgcaa ctcctatagc aaataagata tccaagtttc caaaaacggc atttccagct
     3301 gcaccaatta ctgggatgtt cagcatatcg tcagctgata aacgcatgat caatcccgct
     3361 aatggcatcg ctgcaactgg aagtagcatc gctcttccaa gcttctgcac atttgattta
     3421 acattcattt ttattcctcc tctattaaat atcctcatat gttactaact caatcaaagg
     3481 atcatttttc actttttgag caaatgctga atgaattaaa aattcgactt ggtgggtacg
     3541 aggatacaca aaacttgaac catttaacaa ttcctgatcc aaatacgctg cgtgacacat
     3601 gacttcgatc gttttatctt ctctcaatat catgagtaaa cttgccaaat attcttccac
     3661 actcgtattg atttgtcgtt ttttttcatc agtttctcct acgacatcga aacaaggctc
     3721 aaaataatcg acatgacgaa cttcatcttt tcgttcaaag ttgctccgaa ccggcaaatc
     3781 atattttttt gctaatgtaa caactacctc ctgattttca tccaaagtat gagtatgatg
     3841 atgactgtct aaatgagttg gtatgatccc cgcatgataa attttttgaa tctgagcatt
     3901 ccattcctga tacagttgat ctttgtcaat gttaaatgga tgctgataat aagataagga
     3961 tctgaactct ccgctttcga taagagtatc gacagttttg aggagtggtt tgccacacgt
     4021 caatgttaaa tggactccta cacctagcct aggcatttcc tttcttagtt tcacagcatg
     4081 ctcgaaaccc ggcatatttg ccatcaatgt agttgatgtc aaaattcctc gctgataagc
     4141 atccataatc ccataattca caccatggct atagccaaaa tcatccgaat taaaaatcac
     4201 ttttcccatt tcattctcct tcttttttct actaaaatag gcgcaaccaa ataatcatag
     4261 tccatttact atgtgtcatt tggttgcgcc tatagatagt tacgccctta ttttaataga
     4321 ttcttgctag atgaattgcc aaatagatga tttcattttc ttgaatggca agttgaaatt
     4381 gctgttcgat gatatcttta atctttagtg ctgtacaata cgctttgcta tgtttaaatg
     4441 tttcagtaaa aaaaccaata ttttctactg gcgtttcttc ctgataaatc aagcgtttaa
     4501 ccaggaattt gatatgaacc attaagcgat gataagtcag tgattcttta tcataaacgt
     4561 ttgaatagga tttttctaga tgggacaaaa tcgtggtcgt catttctact atttttaagc
     4621 tctttaaact attaggctct cctagctcag cattaatgat atgcatagtg atgaatccta
     4681 cttcatttga ccctaactcg gtttgatatc gatgattgat tcgctcgacc acttctactg
     4741 ctatatcata ttcatttgga taaaggaatc tcatttctgg caacataatt gctgtgacat
     4801 ctctatcttc tctttttcgt tgaatagcaa aataaatatg atctgtcaat gtaatataga
     4861 tatttgaatc taatggagag gagatcctag ctgttatcag ttcaatcact tcttccgcaa
     4921 aatccataaa ttcgattgga atctgcgaga ctatctcact gaatcgtttg atcttttctt
     4981 tagaatctaa tacaaataat ttgtcagcat cctcaacatc aaatggctta ctcactttag
     5041 cagaaaagcc aatccctttc cccatcgcaa ttttttctat tccatcgtca tcgatcaata
     5101 cagcattatg attcagcttt ttcatcacat acaccgttaa ttcacctcct ctatatgata
     5161 aaacaaaaca acttgcagac tccttcatac atatttttgt ctccaaacaa tttttaaaga
     5221 caaaaaacga cctaagaaag aaccctttct taggtcgtgc ctaattgaat agtagcaccc
     5281 tacataattt acaaaaatag tttaacacat aataaaagcg ttatcaatat cttttttact
     5341 cgttttatga taatacttgt atcgaattga tttggaggta attacataaa aacaaaaaaa
     5401 atatagcaac tctctataag aaagttgcta tattgataat tataccggcg gccggggtcg
     5461 aaccggcacg tcctcaacgg acactggatt ttgagtccag cgcgtctgcc aattccgcca
     5521 cgccggcaaa tttaaaaata ataactgggg tagctggatt cgaaccaacg catgagggag
     5581 tcaaagtccc ttgccttacc gcttggctat accccaagaa taaagggcga atgatgggaa
     5641 tcgaacccac gaatgccgga gccacaatcc ggtgcgttaa ccacttcgcc acattcgcca
     5701 tcataaaaga tgttatttaa tttctcaata aaaaactaag atgaagacat cttagtcaat
     5761 accggcggcc ggggtcgaac cggcacgccc tcaacgggca ctggattttg agtccagcgc
     5821 gtctgccaat tccgccacgc cggcaagttg atttattagt actaaggcgg taaccggatt
     5881 tgaaccggtg atgaaggttt tgcagacctc tgccttacca cttggctata ccgccattat
     5941 actattatca atctataaaa taaagaataa attatagaaa ctggggtagc tggattcgaa
     6001 ccaacgcatg agggagtcaa agtcccttgc cttaccgctt ggctataccc caagaataaa
     6061 aagggcgaat gatgggaatc gaacccacga atgccggagc cacaatccgg tgcgttaacc
     6121 acttcgccac attcgccatg gcaggggcag caggaattga acccacacta acggttttgg
     6181 agaccgctgt tctaccttta aactatgccc ctataaaatg gaggagagtg gattcgaacc
     6241 actgaaccct aaggaacgga tttacagtcc gtcgcgttta gccacttcgc tactcctcca
     6301 tggtggcgcg ggacagaatc gaactgccga cacacggagc ttcaatccgt tgctctacca
     6361 actgagctac cgcgccatat tttaaaatta ttaattgaat aacaagaacg acggttccga
     6421 cgggaatcga acccgcgatc tcctgcgtga caggcaggca tgttaacccc tacaccacgg
     6481 aaccagttca ttatggaggt taacgggatc gaaccgctga ccctctgctt gtaaggcaga
     6541 tgctctccca gctgagctaa acctccaaca ttcaattaaa atgacccgta cgggattcga
     6601 acccgtgtta ccgccgtgaa agggcggtgt cttaaccgct tgaccaacgg gccaggtaaa
     6661 tttgctacgg agagtaaggg attcgaaccc ttgagacagt ttggcaccat ctacatgatt
     6721 tccaatcatg ctccttcggc ctctcggaca actctccaga gtgaaagaag ctcaatactc
     6781 tttctcaaaa tgag
//
LOCUS       DACEXQ010000144         6791 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.144, whole genome shotgun sequence.
ACCESSION   DACEXQ010000144 DACEXQ010000000
VERSION     DACEXQ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.144"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..939
                     /locus_tag="IXH04_002013"
     CDS             <1..939
                     /locus_tag="IXH04_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ1852591.1"
                     /translation="EWKETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLA
                     IDILTFLTGISSRDSEKFPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHI
                     ESFVDYDFAHLLFSDGSVTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAM
                     LIVISTFALDFIHSDRSIFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVT
                     QNADDLLDEKMKNNIGLKFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCL
                     VSDIYGRVGVMQFHPIFEELLHAFDTRPPVRNEVEE"
     gene            936..2963
                     /locus_tag="IXH04_002014"
     CDS             936..2963
                     /locus_tag="IXH04_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852592.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            2960..3982
                     /locus_tag="IXH04_002015"
     CDS             2960..3982
                     /locus_tag="IXH04_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ1852593.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            3999..4928
                     /locus_tag="IXH04_002016"
     CDS             3999..4928
                     /locus_tag="IXH04_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1852594.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            5173..5289
                     /locus_tag="IXH04_002017"
     CDS             5173..5289
                     /locus_tag="IXH04_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAQ1852595.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            5305..>6791
                     /locus_tag="IXH04_002018"
     CDS             5305..>6791
                     /locus_tag="IXH04_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ1852596.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVT"
BASE COUNT         2263 a         1178 c         1400 g         1950 t
ORIGIN      
        1 gaatggaaag aaaccttacc tgatattgcc catgaaatca atattgttaa tctcacgagt
       61 gagaacgaca ataaagggtt acttgaccct tatgtgattt taaaactccc gaaagattca
      121 gaaagccttg ccattgatat tctcacgttc ttaacaggta tttccagccg agatagcgag
      181 aagtttcctg tcctgcgtaa agctattcgg agtgtaactc aaagcaaaca acgagggttg
      241 ttattggtga ttgatgaatt gcgaaaagaa gatacgccca tcagcaatag tattgctgac
      301 catatcgaaa gctttgtcga ttatgatttt gcccatctgt tattcagtga tggcagtgtc
      361 acccaatcca ttagcttgga aaaacaactc aatattattc aagtggcaga tttggtctta
      421 cccgattcca atagctcttt tgaagaatac actaccatgg aattattaag tgtggcaatg
      481 ctgattgtca ttagtacctt tgctttagac tttatccact ctgaccgttc catttttaaa
      541 attgttgacc ttgatgaggc ttggagtttt ttacaagtgg cacaaggaaa agctctatct
      601 atgcgattgg ttcgagcagg acgtgccatg aatgcagggg tctatttcgt tactcaaaac
      661 gcggacgatt tactagatga aaagatgaag aataatattg gcttgaaatt tgcctttcgt
      721 tccactgacc tagtggaaat caaaaagacc ttagaatttt ttggtgtgga caaggaagat
      781 gaaaacaatc agaaacgctt acgtgaatta gaaaatggac aatgtttagt ttcagacatc
      841 tatggtcgtg taggggttat gcagtttcac ccgatttttg aagagttgct tcatgccttt
      901 gataccagac cgcctgtgcg aaatgaggtg gaagaatgac acaaaaacaa aagaaatttc
      961 ttcactatag ttggataacg ctcctttgtg tgggcgttat tttactttta ttaggcacgc
     1021 tgggcaatgc agtacaagcg acaggcttag ttgatgaaac cattgataca agcaatgaat
     1081 actccaagta tggtttgaat caatatcagc ttgattatta tgtggacaat agctggggct
     1141 ggcttccatg gaattggagt gatggcatag gacaatcggt gatgtatgga ttgtatgcca
     1201 ttactaattt tatttggaca atttctcttt atctatcgaa cgccacaggt tatttggtga
     1261 aagaagccta tgccttagat ttcattagtc aaacatccca agcaattggc gaaaacatgc
     1321 agacgattgc aggcattaca ccgaatggct tttccagttc agggttttat gtcggtttcc
     1381 tccttctttt tattctcatt gttggaatct atgttgccta tacaggattg gttaagcgag
     1441 aaacgacgaa agtgatacga gctgttttaa actttgtcgt ggtgtttatt ctatcggctt
     1501 cctttattgc gtatgcaccc gattatattg cgaaaatcaa tgatttttcc aaagatgtca
     1561 gtaccgccag tctgaatatt gggacaaaaa ttgttcttcc taacacggat agtcaaggga
     1621 aagacagtgt ggacatgata cgtgacagct tattctctgt tcaagtaaag caaccatggc
     1681 tattgcttca atatggctcc actgatgtag aagcgttagg ggaagaacgt gtggaaagtt
     1741 tattgtctac cagtccagat acaaacaatg gtgaagatag agaaaatgta gtgattgaag
     1801 aaattgaaga tcatgacaat acctatttaa ccctaccgaa gacgatttcc agattaggaa
     1861 cagtcttctt tttgtttgta ttcaatattg gtatctcgat ttttgttttc cttctaacag
     1921 gaatcatgat tttttcgcaa gtgctattta ttattttcgc tatgtttttg ccaatcagtt
     1981 tcctattaag tatgttgccg agttttgatg gcatgggcag gcatgccatt accaagttat
     2041 ttaatgtgat tatgacaagg gctggtatta ccttgattat taccgttgcc tttagtattt
     2101 ccactatgct ttattcgctg tcagcaagtt ctcccttttt catgattatg tttttacaaa
     2161 ttgtgacctt tgcagggatt tattttaaac tgggcgattt aatgagccta ttcactttac
     2221 aaagcaacga ctctcaaaac atgggcagac aagtgtttcg cagacctcgt caaatgatga
     2281 atcgacaaac cagaagattt caacgcagta tgagtaagat ttttcgtgga aaagcagggt
     2341 caacgaagca aaagacaatg acaacaaagc agaatcaaac caataaagct aaccatactc
     2401 gtcaaaatga acggactaca ccaaagaaag aaacagcact ttccaatgtg aaaaagcaac
     2461 aaggcggtat cgggcaacga ttaggtgaaa agacagccca tgtattggac acgaaaaatc
     2521 gtctagtcaa taaaagaaag caggcaaaac aacagataca ggcgacacca acccatgtca
     2581 aatatgccct tcatcaagga aaagagaatg tcactcataa tgtaacagac tttaaacaat
     2641 ctatggcaga taccaaagct caacgcaaac aacagcgtat gaaccagcag gagcaaaaac
     2701 gaaagaacat tgctcaaaag cgtttggagc tggataaagc aaaagaaaaa agacgaaaag
     2761 aaaacccatc atctcctgtt cagaataacc agcagcatgt tcgcaagcgt cagcaaaccg
     2821 tcacgcctgt tttgtcacaa aacactaaac gtgaaaatca tcagcgacca ttgaaaaaag
     2881 agcaaaaaca aaagacacca ccgaagcaat tcaatcagag gaagctaaat aaaagaacgg
     2941 ataagcgagg acaacaaaca tgaagctaaa aataacagtg atttgtagtg tggtcttctc
     3001 tctcttcttt tttctcttat tattcctagt cattttcttt gcagatgata cggacagtgg
     3061 cgaaaacaac aaggattctt ctattccaca aggcggtgtg accgtttcac cagaagtgtt
     3121 ggcacaccgt ccgttgattg aaaaatatgg taaggaatat gggattgaag actatgtttc
     3181 ttatatctta gccattatgc aagtggaatc gggcggtaca gcagaagatg ttatgcaaag
     3241 ttccgaatct ttgggtttac cgcccaacag tttgagtacc gaagagtcca tcaaacaagg
     3301 ggttaaatat ttcagtgaat tattaaccag tgcagaacaa caaggagtgg atatagattc
     3361 tgttatccaa tcttataatt atggcggtgg ctttttaaat tatgtgagga gtcatgggaa
     3421 aaaatatacc tatgaattag ccgaacagtt ttctaaagag aagtcgggtg gtcaaaaagc
     3481 agactatccc aatcctatag ccatacctgt gaatggtggt tggcgatata actatggaaa
     3541 tcagttttat gttcagttgg tttcacaata cctaaccgac acttccccaa cagaatttga
     3601 tgatgaaacc gtgcaagtca ttatggacga agccttgaaa tacgaagggt ttccctatgt
     3661 ctttggaggg gcgtcgccta ccacctcttt tgattgtagt ggcttaatac aatgggtgta
     3721 tgacaaagct ggcatttctc tccctcgtgt cgcccaagat cagtatgacg ctacgcaaga
     3781 aatctctatg gaagaagccc aagcaggcga ccttatcttc tttcattcaa cctataatgc
     3841 aggcacatat gtgactcatg tggcgatata tttagaggga aatcgctttt atcatgcagg
     3901 cgacccaatc ggttatggcg acttaagcag tcgttattgg caagaccatc tgattggggc
     3961 tagacgtgtt attcataact aagaaaggaa tgaatcaaat gattataaaa atcgagcgaa
     4021 aagaaaagaa ggagaaacca aagaaatcta aaaaaccgag caagcaaaga aaagttccga
     4081 tgattaaagt tggcacgcat aagaaattaa cctttgtttt atgggtttta ttgattggta
     4141 gtgtcggatt tggaatctat aaaaatttta ctgccattga cactcatact gtccgtgaaa
     4201 cagaaatcat taaacagcag attgtcgata ccaaccaagt agaaagtttt gtgaaatcct
     4261 ttgcgaaaga ctacttttcg tggcaacaat cccaagaagc cattgataaa cgaaatgaaa
     4321 aattgaccca ttatttgacg gaagaattgc aggtattaaa tgaagaaatg attcgtaagg
     4381 atattcctac tagttctagt gtgaacgata tacaggtgtg gcaggtttct caagtgaatg
     4441 agaacacctt tgaggtactg ttttctgttg aacaagttat taccgaagac aaagacaagg
     4501 aaactatatc gtctagcttt catgtggttg tccatataga tgaatcagat aatatggtta
     4561 ttatcaaaaa tccgacaatg agtaagaaac ctcaaaaatc ggactaccaa cccaaacagc
     4621 tagaaagtga ccatacagta gatacagaaa cgatggacga aatcactagc tttttagaaa
     4681 cgttcttcca gctctatcct accgccacag aaaaagaatt gacttactat gtaagtaatc
     4741 atgtgttacc tatgattaac aaggaatatg ttttcgagga attggtgaat cctatcttta
     4801 ccagaaaaga taaccaagta atcgtgaatg tcgctgtgaa atatttagat caagaaacaa
     4861 aggcaaccca aatctcgcaa tttgagttga tattagaaaa acaagataat tggaagattg
     4921 taaaataaca aatattggta catgattaca gatactttgt aatcatgtac tctttttgat
     4981 aaaaaattgg agattccttt acaaatatgc tcttacgtgc tattatttaa gtgactattt
     5041 aaaaggagtt aataaatatg cggcaaggta ttcttaaata aactgtcaat ttgatagcgg
     5101 gaacaaataa ttagatgtcc ttttttagga gggcttagtt ttttgtaccc agtttaagaa
     5161 tacctttatc atgtgattct aaagtatcca gagaatatct gtatgctttg tatacctatg
     5221 gttatgcata aaaatcccag tgataaaagt atttatcact gggattttta tgcccttttg
     5281 ggtttttgaa tggaggaaaa tcacatgaaa attattaata ttggagtttt agctcatgtt
     5341 gatgcaggaa aaactacctt aacagaaagc ttattatata acagtggagc gattacagaa
     5401 ttaggaagcg tggacaaagg tacaacgagg acggataata cgcttttaga acgtcagaga
     5461 ggaattacaa ttcagacagg aataacctct tttcagtggg aaaatacgaa ggtgaacatc
     5521 atagacacgc caggacatat ggatttctta gcagaagtat atcgttcatt atcagtttta
     5581 gatggggcaa ttctactgat ttctgcaaaa gatggcgtac aagcacaaac tcgtatatta
     5641 tttcatgcac ttaggaaaat ggggattccc acaatctttt ttatcaataa gattgaccaa
     5701 aatggaattg atttatcaac ggtttatcag gatattaaag agaaactttc tgccgaaatt
     5761 gtaatcaaac agaaggtaga actgtatcct aatatgtgtg tgacgaactt taccgaatct
     5821 gaacaatggg atacggtaat agagggaaac gatgaccttt tagagaaata tatgtccggt
     5881 aaatcattag aagcattgga actcgaacaa gaggaaagca taagatttca gaattgctcc
     5941 ttgtaccctg tttatcatgg aagcgcaaaa agcaacatag ggattgagca gcttatagaa
     6001 gtgataacga ataaatttta ttcatcaaca tacagaaaga agtctgaact ttgcggaaat
     6061 gtcttcaaaa ttgaatattc ggaagaaaga caacgtcttg catatgtacg cctttatggc
     6121 ggaatcctgc atttgcggga ttcggttaga atatcggaaa aggaaaaaat aaaaattaca
     6181 gaaatgtata cttcaataaa tggtgaatta tgtaaaattg ataaggctta ttccggggaa
     6241 attgttattt tgcaaaatga gtttttgaag ctaaatagtg ttcttggaga tacaaagcta
     6301 ttgccacaga gagagagaat tgaaaatccg ctccctctgc tgcaaacaac tgttgaaccg
     6361 agcaaacctc aacaaaggga aatgttactt gatgcacttt tagaaatctc cgacagtgac
     6421 ccgcttctac aatattatgt ggattctacg acacatgaaa ttatactttc tttcttaggg
     6481 aaagtacaaa tggaagtgat tagtgcactg ttgcaagaaa agtatcatgt ggagatagaa
     6541 ctaaaagagc ctacagtcat ttatatggag agaccgttaa aaaatgcaga atataccatt
     6601 cacatcgaag tgccgccaaa tcctttctgg gcttccattg gtttatctgt atcaccgctt
     6661 ccgttgggaa gtggaatgca gtatgagagc tcggtttctc ttggatactt aaatcaatca
     6721 tttcaaaatg cagttatgga agggatacgc tatggttgcg aacaaggatt atatggttgg
     6781 aatgtgacgg a
//
LOCUS       DACEXQ010000145         6776 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.145, whole genome shotgun sequence.
ACCESSION   DACEXQ010000145 DACEXQ010000000
VERSION     DACEXQ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6776
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.145"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..251)
                     /locus_tag="IXH04_002019"
     CDS             complement(<1..251)
                     /locus_tag="IXH04_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852597.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVEL"
     gene            complement(436..834)
                     /locus_tag="IXH04_002020"
     CDS             complement(436..834)
                     /locus_tag="IXH04_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAQ1852598.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(831..1604)
                     /locus_tag="IXH04_002021"
     CDS             complement(831..1604)
                     /locus_tag="IXH04_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAQ1852599.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1601..2284)
                     /locus_tag="IXH04_002022"
     CDS             complement(1601..2284)
                     /locus_tag="IXH04_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAQ1852600.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2451..3338)
                     /locus_tag="IXH04_002023"
     CDS             complement(2451..3338)
                     /locus_tag="IXH04_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1852601.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3335..4204)
                     /locus_tag="IXH04_002024"
     CDS             complement(3335..4204)
                     /locus_tag="IXH04_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1852602.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4341..5627)
                     /locus_tag="IXH04_002025"
     CDS             complement(4341..5627)
                     /locus_tag="IXH04_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1852603.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5900..6776
                     /locus_tag="IXH04_002026"
                     /pseudo
     CDS             5900..6776
                     /locus_tag="IXH04_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292017.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
BASE COUNT         2118 a         1477 c         1115 g         2066 t
ORIGIN      
        1 agttcaacca ctgctcgatc ccaagattca agagaaagct cgatataatc ttttactggt
       61 aattccaatt ctaccttaaa aaagtgcaaa acagccgaca gcccttctgc ttgatagatt
      121 tctcctaatg ttagcccctc tagatggaac tctggtgaaa aatgcagcaa tgttttattt
      181 tcctgcaatt gaacatatag aaatgtagat cgctcaatca gagtatcttt taaaacaact
      241 aaaaaattca tttttctccc ccttttttca tcatttttaa aatagcatat tttgaaaaga
      301 aagtctgatg cttagagtag aatttcataa aaatttacta tttcataagt attcacgaga
      361 aaaatacaaa caaaaaagcg gaaataacgc tcatccgcct tcttgtttgt gtttatttcc
      421 tcttcaaacg cttgtctatg attcagactt ttctctatcg ttattttcat ctaaaagttc
      481 ttgcattccg ataatttctc gatattgatg aatcttagtg ataccaatga ctttgtctac
      541 atcaatacta aaacaaatac cattacctgg aatatcgatg ttgatgcctt gaccaatcgc
      601 atccattact ttattcgtca aactatcggg tactaatgtc aacacaatat ctttttctgg
      661 cgaaatgtcc aaacctaaaa atttccctga atcatgtaca ccagaacctc ttccatgtaa
      721 gatcgttccg cctgctgcac ctgcttcttt tgaaaagtcg ataacatctt cgcccattcc
      781 tccgtcaacg atcgtcacaa tcatgtgaag attcaatgct acaagatctt tcatttgctt
      841 tcccccaatt tttgcaatct ttctttttcg attctgtctt tttcatcttt tcgtttataa
      901 atatatccta gtactaaagt ggataatatt ggtgtcaatg caatcattgc aaccacacca
      961 aacccgtcaa tgatcggatc tcttccttca atttgtgcag cgattgccac gctcattgat
     1021 aaaataaatg tcgaacacat cgggcccgtt gctactccac cgttatcaaa agccattgct
     1081 aaaaagatct tgtccacttt tcgtcctaaa ataaatgcta gcccgtatcc tgggatcaag
     1141 aaataataaa gagagaatcc gacgagcaaa cgccacatag acaatcctac tgctgcgccg
     1201 atcccaattg aaattaccat cagcatgatt ttcgatcgga ttctcccctc gctcaattct
     1261 tcaacctgtt tgatcattac atgaatcgcc ggctcagcaa accctactaa gaaccccatt
     1321 aaaaagccaa ggggaaccag tatataattg taaggcaatc ccgcaatagc actgcctaaa
     1381 tgctgtccta ccggtacata agccatattg actccgtgca ggaaaaggat caacccaata
     1441 gtcgttatca agaaaccttt cataatgtca aagaaacgct ttttgcgcat acgaaaacta
     1501 atgatattca ttaaaacaaa taggattacg ataggtgcca ctgccaaaat gacttccatg
     1561 gtaacgattt cgattccatc tatcacttgc tgccagaaac tcattggaag atcaccccca
     1621 aaatcatcac ggcaagaatc ggccctaacg aagcaatacc gaccattcca aaactgtcat
     1681 tttcattttt cttttgtttg atcatactag tcatccctcc ggcaagggcc aagataaatg
     1741 gaacagttac tggaccagta gtcactcccc ccgaatcaaa agcaatcggc ataaattcgg
     1801 aactggtgaa aaatgaggcg atcaaaacgc ctatgtagcc tatagccatc aattgataat
     1861 aggatagctt gaatacgacc cgaagcagcg caaaggctag aaatactcca gtgccgacgc
     1921 tgacgatccc aatcagcaac actctgccaa tctctccgtc agatatatca tatacttgtt
     1981 gtcccaaaac ttgcacggaa ggttcagcaa tcgtgatcac gatcccgata gcaaatccca
     2041 aactgaccaa aacggtcaaa cttttctttt tgatcatata tttccctact agatctccga
     2101 cttccatcat agaataatct gctccaaaca aaaagagcgt cattccgatc atcatgattg
     2161 ccgcaccaac cagaaatgaa gcgaggtctt ctccttctaa cggtgcaaaa acaaacgtta
     2221 aaatgacaat caggacagtc atcggcaata tagcagcaat cacttcttta aaatttttta
     2281 gcatgtatcc tcctccttta ctctttaagt ttaccttaaa atgaaagagt tttcgagcac
     2341 aaacttaaaa cgttatcatt taatttttct caacataaaa taacacacgt cccaaaagta
     2401 tctttttact tggaaaaatg actttgaagg aacgcgctta ttgtgttttt ttattttaca
     2461 gagccgatca ttccttgaac gaattgcttt tgcaaaacaa aaaatacgat ggcagttggt
     2521 aaagtagcga tgactaatcc agccatgatc attccatagt ctggtgtata ggaagcaccc
     2581 atagcagata aaacaagcgg aaccgtccgt ttatcaggag attgcaaagc tactaagggc
     2641 cataaataat tgttccagct actcatgaat gtgatgattg cagccgcggc ataggtattt
     2701 ttagccgtcg gcatatacac tctaaaaaaa atacttagtt cttttagacc gtctaatcga
     2761 gcggcttcta ctagatcttt tggaaaagat ttaacatttt gtcgaaagaa gaagatcaaa
     2821 aaagcagtgc taacagatgg caggatgaca accaagaaac tgttgattcc caaaaccgtt
     2881 ccgttcaatt gtgaaaacat tcgatataaa gggatcatca atgccgcaaa aggaaccatc
     2941 atcgatagca gtagaatatt gaaaattagg tcttttcgtt tactttgata aatctcaaat
     3001 ccatatcctg ccattgacga aatcagcaat gcaaacacag tggtcaccaa agcgatcatc
     3061 gctgaattcc aaagggaacg agtaaaattc agctcattgg aaaacaaatt ttgaaaattg
     3121 atgatcaagt tactgccaat ctttattttt cccgatgtga tatccacggg tgtattcgtc
     3181 attcccaaaa ccatccagac aaaagggaaa atggaaataa atgataggat gcttaatgct
     3241 gtatacttta atactgaccc aattgtcgaa cgaagttcta atgcttgttt tttgacgttg
     3301 gggcgttcga ttgtttcttt ttctaccata tttttcatcg actttctcct cctactttcg
     3361 tttggattat cgacaatatc acaataaagg aaacaataac aaaggaaact gctgccgcat
     3421 atccaaaatt aggcgtgaat ttataactca aattatagat gtattgagat agggtggtcg
     3481 ttgcatttgc tggtcccccg ccagtaatat tttgtacttc atcaaaaagc tgcaatgtcc
     3541 cgatcgtcga agtaatggaa gtaaacaaaa tgattggttt taaatttggg atcgtcactt
     3601 tgataaactg ctgccattta gaagcaccgt caatagatgc tgcttcataa atctccgaat
     3661 caatattttg catgcctgaa aggaaaaaaa tcatattgta tccagtccaa cgccaagtga
     3721 tcgaaagaat gatcagaact ctcgcccaaa ttggatgagt caaccagcca atcggtgtgt
     3781 caatcatacc aatgcccaac aacaagttgt ttacaaggcc agattgagaa aatagacttt
     3841 tcatgatcag agaataactg accatcgaag tgatacatgg aagaaagata gcggttcgaa
     3901 acaacccttt atatttcaac tttttatcat ttaaaaggtt ggaaataaaa agtgctagaa
     3961 acaacatgat cggtacttgt acaagtaaat acaggaatgt attaaaaaaa gcttttttaa
     4021 aagttgcatc tgtcaacatc cgctgataat tacctaaccc gttaaatgtc atattgacag
     4081 ggggaccact ttgaaaagac gtgatcaaag ccaaaaccat cggaataaaa accatgattg
     4141 atatcaaaat gactggcaag attaaaaaag catgcccgtt tttgaataaa tctttctgct
     4201 tcattctttt ccctccaatt ctatcattga ttatctacat gaaaagacaa gaaagacaat
     4261 atctgtaagg cagacgtgat cttttttgtc ttttcgtttg tctgatgaaa aagcgaagaa
     4321 attcactttt ttaccaccga tcaattagcc aattgtgctt ctacttgttt ttgtgtatct
     4381 gctagtacct tgtctgtctc ttctccattg atgattcgat gaagagcttc agctacgact
     4441 gcttcaattg cataggtctc atgtccgtaa ttcacttctg gtatttcaga tgtccatgtt
     4501 gaaaaatcgt caaagatctt ttgaccactg taaaattcag aagcttcttg ataagcttct
     4561 tgttcattcg cgctaagcat cgtagatact agtccgattt ctttcgcaag agtgcccatc
     4621 agttcttcat tcgaagcaaa cgttgcttct aggaaatctt tcgctgaatc ttctccagct
     4681 actcctttga tcacatacca gccagcgccg ccaaggttag aagcttgcgc tttttgcaga
     4741 ttttctggaa gtgcaggtat tggtgcaatc ttccattttc cagattggtt ttctgctcct
     4801 tggatcgttg aagaatacca agcgccagtt gggctagaag ctactgctcc tgattgtatc
     4861 gcagttactc cagcattcca gtcagaggtt tgttcgacta gatcttcttt caaaagagtg
     4921 gcaaacaatt caagaccata ctttaaagat gcattgtctt ggatcgtgac tgtctcccca
     4981 tcttcagatg tataccattc tcctgcctgt tgcataatca tccttactcg accaaggtca
     5041 gaaggattga cttcagcgat tttctttccc gtcttttctt tgacatctcg cgctacttga
     5101 atatactcgt cccacttcag attctccata tcctcttctg tataacctgc ttctgccatc
     5161 agatccgtac gatagaagtt tgctgttacg cccgaatcga aaggaacacc gtatatttta
     5221 tctcctactt tgtttaccgc aaatttatac ggtgcaaaat gatcttcctt tacaatagca
     5281 gacaaattag cgaatgcatc tggataagaa gtcaaatagc cttgtatcct ataatcttca
     5341 atcaagacga tattgggcaa tccttctgta ttcccgctcg ctaaagcggt gttcagtttc
     5401 tgaacgatat catcctgaga catcgtaaca acttctatct ctgtttcttc gttctcataa
     5461 actttttttg cttcattcac tgctttgata ttgaatgtct catcccacgc ccagacagtg
     5521 actttttctt ctttgtctgc tgtactacta gcacttccgc atcctgttaa gaaacctgat
     5581 gctgccgcta aacaaacgac tccagcaatt aactttctga tgttcatttt tatccgctcc
     5641 ttttccatcc attagttgtt tacgtttgct acattcatca taccaaaatt tgaaaacggt
     5701 ttctttatta ttcagatact tcaaaaattt ctcctaaaat aataaaaaaa tacaaatcaa
     5761 ctatgacaaa aggaataatc ttagcatcta gaccaaagat cgaaatggtg aggtttcttt
     5821 aaaataaaaa tatccttgca ttttctccta gaaataagta tttttaaatt acaagactat
     5881 tcttaaggcg ggtaaaaaaa tgccaatacg cgtaattctt atcgatgatg aacagacgat
     5941 tttagatgga atgaaacagt tgatcaattg gtctgagcac gggtttcaat taatagggac
     6001 tttttccagt ccgctgaaag cgcttgattt ttctttggta catgcaatcg atatcgtggt
     6061 cacagatgta atgatgcctg aattaaatgg aatcgaatta tcgcggctat taaaacagca
     6121 acaacctgaa acacagatcc ttatcctctc tagctatgat gaatttgata tggttaaaga
     6181 ttcttttaaa gaaggcgtcg ctgattatct tttaaagcct cgattgagtc ctgatttttt
     6241 tctaaattcc ttaactgccg tttcacaaaa aatcaaaaaa aagcccaaca aaagcccaat
     6301 caccaatcgt tatgagcaga tctgcgagaa tctttcacag cagattgctg gacaaactcg
     6361 tccattgctt gaccctcttc tttttgaaca caccttgttt ttctgttata taccgaaaaa
     6421 cggccattca gttcaaaaca aggctctaaa caaaaacagt cgatcaccca ttcattgatg
     6481 gatagcgacg attcgtttac tatttatcct gtttctacct tttcggaaga atccggctat
     6541 gtaatcaata ctgattccaa agatacactt aggaagtttg ttcaaaagtt tcgtcaagaa
     6601 gtttttccag gtgatttttt tgttgtttct gattcttttg caatccaaga tatcaatgaa
     6661 atattccaag agatcagaaa ttgcaccaaa ggacaacgtt tctatcataa gggaaagaac
     6721 cttgcttatc agcatgaact tcttccgtta ttaaccaacc acgagcagca agcaag
//
LOCUS       DACEXQ010000146         6738 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.146, whole genome shotgun sequence.
ACCESSION   DACEXQ010000146 DACEXQ010000000
VERSION     DACEXQ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.146"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            558..758
                     /locus_tag="IXH04_002027"
     CDS             558..758
                     /locus_tag="IXH04_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ1852604.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            905..3694
                     /locus_tag="IXH04_002028"
     CDS             905..3694
                     /locus_tag="IXH04_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ1852605.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLRINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            3741..4268
                     /locus_tag="IXH04_002029"
     CDS             3741..4268
                     /locus_tag="IXH04_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ1852606.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4289..5479
                     /locus_tag="IXH04_002030"
     CDS             4289..5479
                     /locus_tag="IXH04_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ1852607.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYSLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5519..>6738
                     /gene="asnS"
                     /locus_tag="IXH04_002031"
     CDS             5519..>6738
                     /gene="asnS"
                     /locus_tag="IXH04_002031"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAQ1852608.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERT"
BASE COUNT         2254 a         1241 c         1443 g         1800 t
ORIGIN      
        1 tgattatgaa agttgcttgc atttcttaca ttctacagct gcagcttttg cttttactca
       61 tactcagaaa tatgatggac atcatcctgt tatttatgag gtatagatat gagcaaaaaa
      121 gaaaaactaa tctcgttgat cgtattggtt tgccttgttc ttctggtaac tgtcataggc
      181 tatcagaaat accatgagaa catcagacct actaaagaat cttccccagc ctcgaccgtt
      241 gaaacagaac cgataagtag tcagaaagaa taattttttt agtttaaaga agaacatcag
      301 agcaaaaaaa caccaactga acaatatgtc gtttatttta aaaaaagttc ggtttttcac
      361 tttatgtaat cgctgagaaa acttttttta ataaaaaaag tcgaaaaact ctagcttttt
      421 tgtaaacggt atgatatact ataggagtag tttttgttcg gtaatctgtg gtagcttgtt
      481 tcaaataaac atcttccaga tatacaccat tcaagtaatt gcaaaatatg tgttgataca
      541 caaggaggat ttttattatg gaaacaggta cagtaaaatg gtttaactca gaaaaaggat
      601 ttggattcat cactgcagaa aacggaaacg atgtattcgt tcatttctca gctatccaag
      661 gcgacggatt caaaacttta gaagaaggcc aagcagtaac tttcgatgtt gaagaaggcc
      721 aacgcggacc tcaagcaaca aacgttacta aagcttaatt aacctatact aaaaaataaa
      781 cgcccagaca tttgcgtgtc tgggcgtttt tgtctatttt ctttaaaaag cgatgagagt
      841 atttttctgc actttgtagt ataatttcaa aagaacatta gaggcaggag aagaggaaga
      901 tattttgaaa ccaagaactt atgcagtcgt tgatatcgaa acgactggga cgaatccaaa
      961 agaagatcgg atcatccaat ttggctgcgt catgattgaa tcaggtcgga tcacttcacg
     1021 tttttcgatc gatattcatc cagggcggaa gatttcaaaa caaatccagc atttaacagg
     1081 gatcacgaat caacgcgtcc aaaaagcgcc ttatttcgaa gatgtcgccc aaacgattta
     1141 caatcttttg gcagacacca tatttgtcgc gcataatatt tattttgatt ataatttttt
     1201 gaaccatgaa ctcatgcgat gcggacttcc tagtttgaag ataccaggca tcgatacagt
     1261 agaacttgca caaattttct tgccgacgga accaagcttt cgcttagctg atttgtcaga
     1321 aagccttgga tttattcatg agaatcctca tcaggcagat agtgatgccg aagtgacggg
     1381 tcaacttctt ttgctgatcg aagaacggat gagaaaattg ccaatcatca cgttagagca
     1441 gattgcgcga ttaagtagac acacaggtat ggatacgagt cgtttcattt atcatgtgat
     1501 tgaagaaatg aaagaaaaac cagagccact tgatccctca ttagaaatcg tcgatgggct
     1561 agctttgcag aaaaaagaag tggaactatt cacatccgtc cattatggcg agcggacata
     1621 tccaagaaaa aaacaagcaa aagaaaaaat gtttggtaaa acactgatgt atcgaaaaga
     1681 acagaacaga ctgatgaatg cggtctacga ccatttcaca aaagatgaat caaaagattt
     1741 gatgatcgaa gcagctacag gaatgggtaa aacaatcggt tatctgctgc cattgagtta
     1801 tttggctaca cctgaaaaac ccgctgtcat cagtaccgtt tcgctagttt tgcagcagca
     1861 gatcatagaa aaagacattc cattgctgaa tcaattgcta gatcagccca tacaacctgt
     1921 aattatcaaa agctatcgcc actatattga tttacagcgt tttaaaggca cacttgtgga
     1981 accgccagaa caaaaacaat atgctttgta tcaaatggcg gtcttagttt ggctgacgca
     2041 aacgaagacc ggtgatttgg atgaattaca tttaacaaat ttgaaccatt cattctttgc
     2101 agatatcgcg cacagaggta ttggattttt agcaagaaat cagtcatttt acgaacaaga
     2161 ctttgttcgt ttcttgcaaa agaaaatcag gcaaagtaat tttttgattg tcaatcatgc
     2221 gtttttaata caagaaacac agcgaaaaga accattgctt ccagctagtc catttttgtt
     2281 gatcgatgaa gtacatcatt tgccggaaat cgctgaaaaa gtaaatgaca aacgattaag
     2341 aattaactat ttccataaac aaatccagca attagaagaa ccaaatcagc tgtttgaccg
     2401 aatacaaagc tatttaccga aagaacatga agccttacgc tggttatcga tttatcgaga
     2461 agaactttct gcgctatgcg aagcacagat ttgtatggca gaaggattaa tgagtttagt
     2521 gagcggcaag caacctaacg gacagtatcc agaagaagtc atgatgacaa aagagatgtt
     2581 ggatcagctg ccactggaat ctgggacagc cattgatcac ctgcagcttt tttatcagga
     2641 gatccagcag ctacagtcac aaatgcgtct gacttttgag attatccaag aaagctggac
     2701 aaaccagatg cggatcgatt tagcaagttt atatagctta tttgatcaat tagacaagca
     2761 gtacttgctg atggatcaat ggctggaaga ctggcgagat catttgatcc attggttcgt
     2821 tcctgggaac acagcgcagc agatgatttt tcatctccat gattttcaag cggctatgtt
     2881 gccgtcgact gtttggtatc ctcgatatga tcgtattctt tatattggag gaacgctgaa
     2941 gatcggccct aatcgacact atctctctaa gcggcttggc attgaagcag cacctcttaa
     3001 agtgattgcc tctccttatg attatgagaa acaggcaagg atctatgtgc caaaagaggc
     3061 acctgccgtc catcaagtca gcaatcaagc atatatcgat tatttagaga cagcacttac
     3121 aggtctgatc gaacaagaag atcggccaat acttgtgtta ttcacttctc atgatgtttt
     3181 gcagaaggtc tatcagaaaa tgcatctgtc tatgctagaa aaaggacgag aaatactagc
     3241 tcaagggttt ggcggaagcc gggagaagct tttgaaacgc ttcattttgt cagaaaaaag
     3301 cgtattgttt ggcgctgaca gcttttggga aggtgtagat ttgcctgggg atgctttgca
     3361 gatcgttgta gtcacacgtt tacccttcga taatccgctt cgcccgatgg tcaaagctag
     3421 gaacgcttat ttagaatcag aggggatcaa tcctttttac caggaatccg tacctaaagc
     3481 agctttgaag ttacgccaag cattaggtcg cttgataagg acggagacag atcgtggcgt
     3541 attgcttatt ttggatcgaa gactagtcac tgcaaaatat ggtgcacgga tcatccatgc
     3601 attaccgaaa aaagtaccga tcaaagagct agctatggag gaaatctatc aggacatcca
     3661 tgaattctta aagaaaaatg aagaaggaag ttagaggagc tgtctttttt cataattaaa
     3721 atctgctata atgaaaagga atgaagataa aggagcgacg acccttgaag aaaaaacaga
     3781 caaaggaaaa aacgattaat cgcgtgttac ttggcttgat cgtattttta ttagcggtca
     3841 tcgtgatcag ctcgattttt tacttgcgtt ccagtcgtcc ggcggtgcag gcgaagaaag
     3901 aagcagtaga aatcgcaaag aaatatgcaa atcttgagga agccgatcat ttttattggt
     3961 ttactcgaaa gaagacatac ttttcactga caggtacaaa tgataaagga aaagaaatcg
     4021 ccgttatcat tcctaaatct ggtgaaaaag taaaagtatt ggatcaaaca aagggactga
     4081 ctgaaaacga agcaaaacag acaattgctg cggcccatcc tgaaatccaa atcgaaaaag
     4141 ctgctttagg gatgtatgat gatcagcctg tgtgggaagt agtaggaaaa gaaacaaatg
     4201 ggaaactgaa ttattacctg attggctttg aatctggaga tgaagtcaag acaatcaagg
     4261 gaatttaaaa ggtaaaggaa tgaatgggat ggagttatca aaaagaacaa aacgattaga
     4321 accgtctgtc actttggcag ctgcggcaaa ggccaaggca ttaaaagcaa aaggcagaga
     4381 tgtgctgagt ttgactgtgg gagaaccgga ttttgcgaca ccagaaaaca tccaagaagc
     4441 agcaatcgaa gcaatccgca acgggaaagc tagctattat acaccaacag caggaatccc
     4501 agagcttcga caggcaatcg ttgattatct gaagaaaaac tatggacttg tctatgaacc
     4561 ttcacaaaca gtcgtaacag acggagcgaa atttgctttg tatacccttt tccaaacgat
     4621 ccttgacccg caagatgaag tcatcattcc tgtgccttac tgggtcagtt acggtgagca
     4681 agtaaaactg gcagaaggac ttcctgtctt tgtgaaaggg gaagaatcca atagctggaa
     4741 agttactgtt gaacagctag aacaagcacg ttcagaaaaa acaaaagccg taatcatcaa
     4801 ttctccatcg aatccgactg gaatgattta tagcaagaat gaactgcagg caatcggtga
     4861 atgggcagta aaaaatgata ttttgatagt tgctgatgat atttatggtc gtttagttta
     4921 taatggcaat gagtttactc caattgcaac gatttcagaa gcaatcaaaa atcagactat
     4981 catcatcaac ggcgtgtcta aaacatatgc gatgacaggc tggcgcattg gttatgcggt
     5041 gggaaatcct gtgatcatca atggaatgat cgctgttgct tcccagtcaa ccagtaatcc
     5101 gacagcagtc agtcaatatg cagctgttga agcattgaaa ggcgagcaag atacagtgga
     5161 agaaatgcgg aacgcctttg aagaacgcct gaatacgtta tattcgcttg tggcagaact
     5221 gccgggtgtt tctcttgaaa agccacaagg agctttttat ctttttccta atgtaaaaga
     5281 aacgctacgc atgtgtaaat atgaaaatgt cacacaatgg gtagaagatc ttttagaaga
     5341 aacaggggta gcgcttgtga ctggcgaagg ctttggggca ccagaaaatg tccgcatgag
     5401 ttatgctact gatcgcgcta cgttagaaga agcggtacgc agaatcaagc aatttattga
     5461 gagtaaaagt caaaattaat cctaaagaaa ttcaaggaat ataaaaggag agtcatacgt
     5521 ggaacaaatt caaatcatcg attcgaaaaa ccatgtggga gaaacagtaa caatcggcgc
     5581 ttgggtcgca aataagcgat caagcggaaa aatcgcattt ttacaattaa gagacggaac
     5641 agcctttttc caaggtgtag ttgtaaaaag cgaagtgtcg gaagaagttt tccaaacagc
     5701 taaaaatcta caccaagaaa catccgtgtt agtaactgga gaaatccgtg aagacagtcg
     5761 ttcaaaattt ggttatgaaa tcggaattac aaatgtggaa gtggtaagcg agagccacga
     5821 ttatccgatc acaccaaaag aacacggaac agacttcttg atggatcatc gtcatttatg
     5881 gctgcgttct tcaagacaac atgcgattat gcagatcaga aatgaaatca ttcgtgcaac
     5941 gtatgaattc tttaatcaac atcattttgt caaaattgat ccgccgatct tgactggttc
     6001 tgcaccagaa ggcactacgg atctattcga aacaaattac tttgatcaaa aagcttattt
     6061 atctcaaagt ggccaattat acatggaagc tgctgctatg gctttcggaa aagtcttttc
     6121 attcggtccg actttccgtg ctgaaaaatc aaaaacacga cgtcatttga tcgaattctg
     6181 gatgatcgaa ccagaaatgg ctttcactca tcaagaagaa agtttagaag tacaggaaca
     6241 atatgtagca ttcttagtac aaaatgtttt agatcattgc gaatatgcat tagatgtact
     6301 aggacgagac aaagaagtct taaaaactta tacaaaattg ccattccctc gtattactta
     6361 tgatgatgcg atcgaattac tgaagaaaaa tggatttgat gacatcgaat ggggcgaaga
     6421 ctttggttca cctcatgaaa cgttcattgc gaattcattc gaacagcctg tctttatcct
     6481 aaactatcca aaagccatca agccattcta catgaaacca catccaacaa gagaagacgt
     6541 ggtgatctgt gcagatatga tcgcaccaga aggttacggt gaaattattg gcggaagcga
     6601 acgggcaaca gactatgatt tcttgctaga acaaatccgt aatcacggtt tggatgaaaa
     6661 agaatattca tggtatcttg atctacgtaa atacggttct gttccgcact ctggttttgg
     6721 tctaggtctt gaacgaac
//
LOCUS       DACEXQ010000147         6683 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.147, whole genome shotgun sequence.
ACCESSION   DACEXQ010000147 DACEXQ010000000
VERSION     DACEXQ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6683
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.147"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(362..1480)
                     /locus_tag="IXH04_002032"
     CDS             complement(362..1480)
                     /locus_tag="IXH04_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852609.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1951..3258
                     /locus_tag="IXH04_002033"
     CDS             1951..3258
                     /locus_tag="IXH04_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAQ1852610.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3377..4576
                     /locus_tag="IXH04_002034"
     CDS             3377..4576
                     /locus_tag="IXH04_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAQ1852611.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4603..5871)
                     /locus_tag="IXH04_002035"
     CDS             complement(4603..5871)
                     /locus_tag="IXH04_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAQ1852612.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(6034..6680)
                     /locus_tag="IXH04_002036"
                     /pseudo
     CDS             complement(6034..6680)
                     /locus_tag="IXH04_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
BASE COUNT         2017 a         1404 c         1191 g         2071 t
ORIGIN      
        1 aacggtgttc cagaagtagc ttcttcggaa ataagccgaa atttcacaaa aatttgagaa
       61 ataatttttg tcaatctctt cttatttctt gaagctgacc acttctgtca caacctcttc
      121 cttaaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaccaaaca aaaaatatga
      181 agagctattt ttgcttgctg ttcttattac ttgatgcaga acagcttttt cacaacctct
      241 catacacggt gtttaatcag ctgaccttcg gtattagctc aaaaaacgga aaaatatgtg
      301 aaactatttt tccgtttgtt tctctaatac tcaggtcaaa acactgattt cacaacctct
      361 tttaataacg aagaatcgct gtcagactct tttcatctgg cgcatctttt tgatccaaaa
      421 taaacacttt cccgccatta atgatcacat catctgctaa tgcattcaac acttgacgcc
      481 gatcatattc tgtttcagga tcttcaccga aaccatcagc taagtttgac gtagcgataa
      541 ataaatgtga cacttttcct tctttcgcag ctggaacgat atcaactaac tgatcgatga
      601 atttcttatc taagagttta ttgtattctt ttgattcttg agctgcgagt tcacttgaca
      661 gcttcttcgc cccttcttcg atatctctaa aggatagctg cgcaggagaa gcagaaatcg
      721 caacgtctgc acaataatag ggatttttag caattttttt gaacatcgtc tgattttctg
      781 gtaatgcaaa taaataaatc ggccattgtt caggattttc gaaagaatcc ttcaagtacg
      841 tatcaacagc ttggtaatag ttgacccaat cgatctctac ttcttcatct tttggattta
      901 cgccatggaa agttactcca tctcgggctc cgcctcctcc attagaagag aagttcaaac
      961 cgccacctgt cagttcagat cccaatgcag taacgacatc aaccggtgcc ccttctggta
     1021 gagatacttc ttttacttgt ttgttgctga cctcatacaa tttcatggag tcgcggttca
     1081 atcccaataa ataataagaa tagttgaact gcccattttt gataatagcc agtaaataag
     1141 gaagatcact tacatagtat tgatcgtcta ccgggatact caaacgatgg atataggtct
     1201 ggtcagcagt tataatgacc gctacactcg tagttgcact acgccaaaac gaaggatctc
     1261 ctaacaaatt ttcaattttt tcttggaacg gggaccagtc atgttcggaa tattttttct
     1321 caaaacgttt ctttgcttct ttcgcaaaat ttttgaattt gatctgatct ttttccacat
     1381 cttgatgggc gacatgcgta ttcagcatga tcgtgacgaa tggtccttta gcgccttctg
     1441 aagtcaattg tgtcaataat tctttatcct tttttgccat aaacgtaccc ctcctaaaaa
     1501 aatcattcct ttgtttctat ctaaagtcta ccacaacact atttttccat acaataaaac
     1561 gtatctaaaa aaataaaacg gtttctacgg ttttaaattt agctattttt ttaagttatc
     1621 ttcataattt cctcaattat ttagccccag tcttcaacta ttcttcatga aatgctgata
     1681 aactaagaaa aagaataaaa gaggtggatc ttatgcattt gattatgatt ttcagtttat
     1741 taagtattat cagtttattt gctttcagcg cttcccgctt attagatgcg gaaacacagc
     1801 ctattcctgt aaaagtacgt aaaaacgacc gctaaacttc cataacgcgc gttcttatcc
     1861 taaagttttt tcagaaacat tgaaactctc tcccctctct gataaaattt taatttgtag
     1921 cgaaacaaat taattacaag gagatcggat atgtccataa aaaaaacacg tctagtccta
     1981 ttattggcta gtctcattct ttgccttgct tcttttctac cagctgttgc tgtgcaagct
     2041 gaagatacgt tcaaagtaaa tgcaaaagca gcttttgctg tagatgcgca aagcggtaag
     2101 attctttatg atcaagatgg agaaaaaacc atggggatcg cttcaatcac aaaaatcatc
     2161 ggtctttaca tcgtccttga tcaagtaaaa gaaggaaaac tgtcatgggg tgataaagtt
     2221 tcgatttcag attatgcaga aaatttgagt atcaccccag atttatcgaa tgttcctttg
     2281 cataaagaaa acacctacac tgtcaaagaa ttattcgact ctgcaatcat ccagtcagcc
     2341 aatgcttcaa tggtggcctt ggcagaaaaa atctctggca gcgaagctaa attcactgaa
     2401 cgaatgaaag aacaattgaa agattgggga atcaaagatg caacaatcgt taatgcatca
     2461 ggattgaaca actcttatct tggcgaaaac cgaccagaag gcactggaga aaacgacgaa
     2521 aatcaaatgt ctgctcaaga tgtggccatc gttgcccgcc atttgatctt ggatttccca
     2581 gaaatactgg atgtatcaag tactacgacc caaatgtttg gcgaaaatac gcaatcacca
     2641 gtggaaatgg tcaactggaa ctggatgctc ccaggattcg ttaattacaa ggaaggcgtg
     2701 gacggattaa aaactggtac gactgagctt gcaggtgctt gtttcgttgg tacgatcact
     2761 aaaaacgatc aacggatcat cactgtcgta ttgaatgcgg acggccatgc ggaaaacccg
     2821 agtgcccgct tcaatgaaac agaccgtttg atggactact gctatgacaa ttggtcagaa
     2881 aaagagctcg gaaaagctaa cgcaagtatt cctagtctaa aaaccattga tgtaaaagac
     2941 ggaaaagaat caacagttcc tgttgtgctt aaaagccctg taaaagtatg ggtacgaaac
     3001 gatatggata ctggcaaatt aacgatcacg ccaacacttg acaagaagca agtcactgac
     3061 aatgagatcc aagcacctat ggataaaggt acaaaaatcg gtaccgctac gatcaccttg
     3121 gcagacgata aattaggcta tttagaagac aacaatgcac catcaacaga gatcattacg
     3181 gataaaacag tggaaaaagc gaatatattc gtgattggct ggcgacgcgt cgcagaattt
     3241 ttcggcaact tgttttaatt gaacaataag aaaaacatct ttccatcgga aagatgtttt
     3301 tttgcttttt ctagtttaaa taacagcttt tttcatctat tttcgttata ctgtataaga
     3361 aaaaggagtt gaatttatga atctaaccag agatctgatc tttttagttc tctatcttat
     3421 tggggtattc tctatcgttt attggcgaaa acagaagaag gatccttcct tgttctttta
     3481 cggcagtacg tggacattta cggtaatctt aacttatttt gtcgtgttgg aaatttttta
     3541 ttcgaccttt tattttttga ttccattgtt gttctttagt atctttgctt tttcttattt
     3601 cacagaaaaa cgaaggctac tgaatggtct tttattcaat gttttactca tcagtttcgg
     3661 catctacttg tttgtcttgc tgtatgaaac gcaaaatatt tttcttggcg gactgatcgc
     3721 cttgatcacg attccattgc tacttgtcct tttatttggt atttatggat tgattgtttt
     3781 tcttttttgg aacggggtca cagttttacg tagagaatcc cattcattag ctaatctttt
     3841 aacgttgata ctagccattt ttcttacact gtttcttgtt tttgactttt tcttgttaaa
     3901 atatttgccg caatggataa atgctttgtt tttctgcgtt cccctgatct tgatctatct
     3961 atttatcgta ttctacaact ttttgactgt ctcattcttg tatcagttca accggcctcg
     4021 atacaatcaa gacttcatcg ttgtgttagg cgctggattg atcaatggag aaacagtttc
     4081 tcctcttcta gccaagcgaa tcaataaggc aattgctttt tatcgggctc aaagccgtgc
     4141 tacattaaat ccgcccatct tattgatgtc tggcgggcaa ggagcagacg aaaaagtacc
     4201 agaggccatt gcgatgaaac aatatgccat ggaacaagga atccctgaaa gagacattct
     4261 tgtcgaaacc aattcgacaa ccactttgga aaacatgctc tattcaaagg aaatcatgga
     4321 tcagcaaatg aaagggccat accgcgcgat ctttagttcc aacaattacc acatttttag
     4381 agccggtctt tatgcgcggc aagccaaatt gaaagcaaat ggtattggtg caaaaacagc
     4441 tttctactac ttgcctaatg cgtttttaag agaatacctc gccatcttga tgatgaacaa
     4501 aaaaagacat atgatcattt gcggattgct tttcgcaggc tccgcattct tatcgctgat
     4561 tactttactt ttctaaaaaa acaaggtgtg acaaaacata tgtcacacct tgtttttttc
     4621 tttcattctc ttaactattg gcaaaaagag tatccctttt ttgaccgcta cttgctgagc
     4681 agaataaata ccgctgttgc ctgaacacat aaagctgatc aagcagatca taaagaagta
     4741 tactgccgct tgtgaaccga acaattcgat ccccatcaca aagcaggcaa tcggtgtatt
     4801 tgtcgcacca gaaaatactc cgataaagcc caatcctgca agaaaaggaa cagaaacatg
     4861 gagcaaaggc gctagtgctg caccaagcgt tgcaccaatt tcaaacaatg gtgtcacttc
     4921 tcccccttga tatcccgcgc ctaaagacaa aactgtgaag aataacttcc caataaaatc
     4981 atagacatgt gcctgtccat taaatgattc ttgaagtaac ggaagactca atccgagata
     5041 acgttgtgtt tgcaaaatca atgctgctgc cacaactaca gctccaccta tcgtatttcg
     5101 acaaacagga tgaggaatcc attgtgcata tcgcgctttt aaaaagacga tcgaacggct
     5161 gaatacccaa ccaatcaaac caaaagcaat cccagcaaca aaaactttga agaacagcaa
     5221 catactccat tcaggaatca cacccatttg gtaatgtaga tgagaaacac catagctttc
     5281 tgtcatgaaa tttgcaaaca gtgcagcaaa gaagctaggg aagatcgctt ctgttcggac
     5341 tttcccgatt gccaacactt ctaatccaaa taatgttcca gctaaaggtg tgccaaatac
     5401 agaactaaaa cccgcactga ttccactgat aatcaagatt tcccgttctg atttatccaa
     5461 tcggaacaaa cgtgccgtat tatctgctaa cgctcccccc atttggactg ctgttccttc
     5521 acggccaaca gatcccccaa ataaatgggt agttatggtt ccaaacagtg taagtggaat
     5581 aagccgcaac gggatttttt cttctccacc atttccttga tcgatcacta agttgtttcc
     5641 acgactagaa agtccgccgt gatacgcata gagataagca aaaagcgcac cactaactgg
     5701 tagtagacaa aggagccaag gatgtgatag tcggatatct gtcacaactg tcaggctatt
     5761 taagaaaaaa gcagacaagc tccccatgaa tataccgaga atcgctgtga tcaccagcca
     5821 cttcagcata tacactaaaa tgcgtactgg actcggtaat tgtttttcca taaaaaattc
     5881 ctcctcaata aaaaaacgcc tactaaagaa aggggctgtc cccagacttt agtaggcgtc
     5941 attagctatc tttcgttagc atttaaaggc gaacaccatc acctattcac ttccattaaa
     6001 agatagcata tttttctgta aaaaacaata aaattatcgt tctttcttta cacgttcagc
     6061 aaattcatag aaaggagcca atttcgttaa ctgttgatta cattggtcac tatcatgaca
     6121 aatataattc ccctttttcg tatacgtgcc atcagaacct gctttcgttg tagaaagaaa
     6181 aagcgaaaca ttcgatgttt tctgacagat tgcacagatt cccttattta tcgttggtga
     6241 taaagtaccg ctaatccctt ttaatttttc atcttcgtaa tagaccatga attttttctg
     6301 cgtacctgga tcattccagc cgatgtacgt atgttcttgc aagtcgattt cattccaagc
     6361 tggttgtttg agttttttca cttttcggaa taatttctcg atttgtggtt tgctaggctg
     6421 cataaacggc tgaacgattt gcttcagctc ctccaatgct ctttctgctt gcgcttttgt
     6481 cagttttacg tctaaaactg tcgttaaaaa tgtatcgatt ctggcagttc gtgatctaat
     6541 atgtctcgaa tcttttcagc tgctagtgcc tgaactgttt gtaccgtttt tggatcattg
     6601 acagatgagt acgcatttaa taactgttct acttctctta ttataaaaaa atattggtat
     6661 ggttttattg tttgttccat gat
//
LOCUS       DACEXQ010000148         6681 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.148, whole genome shotgun sequence.
ACCESSION   DACEXQ010000148 DACEXQ010000000
VERSION     DACEXQ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6681
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.148"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            4..1458
                     /locus_tag="IXH04_002037"
     CDS             4..1458
                     /locus_tag="IXH04_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1852613.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVDPSH"
     gene            1474..2505
                     /locus_tag="IXH04_002038"
     CDS             1474..2505
                     /locus_tag="IXH04_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ1852614.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGHFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            2512..3699
                     /locus_tag="IXH04_002039"
     CDS             2512..3699
                     /locus_tag="IXH04_002039"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAQ1852615.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            3855..5237
                     /locus_tag="IXH04_002040"
     CDS             3855..5237
                     /locus_tag="IXH04_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ1852616.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            5209..5595
                     /locus_tag="IXH04_002041"
     CDS             5209..5595
                     /locus_tag="IXH04_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852617.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(5795..6463)
                     /locus_tag="IXH04_002042"
     CDS             complement(5795..6463)
                     /locus_tag="IXH04_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAQ1852618.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
BASE COUNT         2081 a         1285 c         1352 g         1963 t
ORIGIN      
        1 attatgaaaa gcaaaatgat ccaacgtgtg tcctacgctt taggcgcttt tggacacgac
       61 acattctatg ttacattaag cacttatttt atggtattcg tgacaagcca gatgttcgca
      121 ggaacagata aggcaacgaa tgcaaaaatg attgctacag ttacaagctt agttgtaggt
      181 atccgcttag ttgaaattat ttttgatcct ttgattggtg gcatcatcga taatactcgt
      241 acaaaatacg gtaaattcaa accatggtta gttatcggcg gcgttgttag ttccgtactc
      301 ttggtagcaa tctttaccaa tttctttggc ttagctactc ataatacgac tttatttatc
      361 atattatttg caatcgtttt catcttattg gatagttttt attcatttaa ggatatcgct
      421 ttttggtcaa tgattccagc tttgagtacg gaaacaaagg aacgtgaaaa atttgctacg
      481 tttgctcgtt tcggctcttc tctaggtgca aatggtacta ctctagtcgt tgtccctatc
      541 gttactttct ttacctatct tgttactcat aaacatattg aaggcgcttc aggctggttc
      601 tggttcggtg tgattgctgc tgttatttca ggcggtactg cgattatcac tgccttgggc
      661 acaaaagaag tcgatactgc gatccgtcag caaaatgaaa aagctgggat caaagatgtg
      721 ttcaaagcag ttgctcaaaa tgatcagttg atgtggcttg cccttgctta tatcgcttat
      781 gcgattgcca atgcagcaac aactgccgta ttattctact tcttcaaata tgtacttggc
      841 caaccaactg aattttggat cgttggtcta gttgctgcct tacttggttt agtagctgtt
      901 ccactattcc caatgcttac acgcgtcatc tctcgtcgct acgtttacct tggagggatc
      961 gcaatgatga tttcttctta ctttttcttt acgatcgcag gttcaagttt gccaatcgtt
     1021 atcatcgggc tagtattgtt ctacttccct caacaattga ttttcttatc cgctttgatg
     1081 acgatcacag actctgttga atatggtcaa tggaaaaatg gtatccgtaa cgaagctgtt
     1141 actctatcaa ttcgcccttt actagataaa atcgctggtg ctttgtctaa cgggatcgta
     1201 gggtttgtcg ctatcgcttc tgggatgact ggtaatgcta ctgctgctga tatcactgct
     1261 catggcgtaa cgactttcaa agcttatgca ttctatgctc ctgctgcttt gatgattttc
     1321 tccgctatta tttttgcttg gaagattaca ttgacagaga aaaaacatgc acaaatcgtt
     1381 gagaaattgg aaaacaaatt ggctccagct gatacaatgg aagatgaact tcacgatgca
     1441 gtagatcctt cccattaaga aaggatcttg aaaatgaaga ttacagaaaa agattttggc
     1501 caaggttatc acttgataac cttggagaac aaaaacaagc tagcactttc tatctctgat
     1561 ttaggtgcta gaatcgtcag tttaaaatca aatgatcgag aattagtttt gggatttgac
     1621 accgctgaag aatatatcga aaaagatccg tatatcggtg cttctatcgg tcgcaccgca
     1681 gggagaatcg aaaatggcca cttttctctc aatggaaaaa cttaccaatt agccacagat
     1741 ccaaaaactg gacataatct gcatggtggt gctcctggat ttgaattaaa aaaatggtct
     1801 tatgtcattt tgaatggcga aaatgaagct tctgtcattt ttactactac aagtcccgac
     1861 ggagaacatg gttttccagg tacaatggat gtggaaatcc gctatacttt gacaaaagat
     1921 aatatctggc gagtgacctc tcgcggcacc agtgaccaag atactttatt caatccgaca
     1981 aatcatgttt acttcaactt gacgggagat gcttctcagt caatcgatca acatgagctt
     2041 tggctcaata gcgaagctta tgctcctttg cgaacagata gtattcctat aggggtaaaa
     2101 gaaaatgctg caggaagtgc tttcgatttt caaataccta aaaaattagc gtccgttttt
     2161 gctagtgatt tggatcaaaa aaatcttgtt gacggaatcg atcatccatt tttcttaaaa
     2221 gaaaccggct tgggtaaaga agccgctcgc ttgactagcc ccgataaaag aatccaagta
     2281 gatattgcta cagatgcttc tagtgtggtt attttcactg caaatttcgg cacagaaaca
     2341 ccagaaatgc gcaaccgaaa actggcacat cacggtggca tcacatttga gactcaaaca
     2401 gctccaggtg cagagcgctt ctcagcattt ggttcgattc atttaaaagc cggatcagta
     2461 tttgaaacag tgacagaatt caaaatcaaa acaagaaagg aataactcat catggaaatc
     2521 aaaagaagct taaacacaaa atttgccgaa acattcggac ctaaaaaaac tacacaatac
     2581 ttctcaccag gacgcatcaa tttgattggt gaacacacag attacaatgg aggccatgtc
     2641 ttccccgctt ccatcactta tggaacgcaa ggtgtagcag cacctcgaga cgataataaa
     2701 atattagttt attccacaaa ctttgacgat atcggtgtca ttagttttac tttagatgag
     2761 ctagaatatg ataaaaaagc aaactgggca aattacgtaa aaggaatggt ccttaaatta
     2821 aaagaagctg gacactcaat cgatcatggg tttgagttat tagtggaagg aacaatccct
     2881 aatggtgcag gactttccag tagcgcttca ttagaattat tagtaggtgt tgtattagaa
     2941 gatctgtttg atttaaaaac agatcgcttg gaactagtcc aaacaggtaa aaaagtagaa
     3001 aatgaattca tcggtgtcaa ttcaggtatc atggatcagt ttgccatcgg cttcggtgag
     3061 ctagatcatg caatcttatt agatacaaat acgctgaaat acgaaatggt tccagtgaaa
     3121 ctagatggtt atgcaatcgt catcatgaat acgaataagc ggcgggaatt ggcagattca
     3181 aaatacaatg aacgccgtag cgaatgcgaa gaagccttgg cacgtttgca aaaaactttg
     3241 cctatctctt ctttaggaga cttagatgaa gaagaatttg aatcaactat tgaccaaatc
     3301 ggcgatgata cattgatacg cagagctcgc catgccgttt ctgaaaatca gcggactttg
     3361 aaggcaaaag ctgaattaga agctgggaac ttagaagcat ttggtcaact gttaaatgat
     3421 tctcaccact ctcttcgcta tgattatgaa gtaactggga tcgaattaga tacactagta
     3481 gatgctgctc aaaaacaaga aggtgtctta ggtgcacgaa tgacaggagc tggatttggt
     3541 ggctgtgcga ttgctcttgt caaagaagaa aatatacctg ccttcaaaaa caatgtctat
     3601 gatgaataca tgaaagttgt cggctatgct ccggaatttt atgttgcaca cattggaaat
     3661 gggacaacaa agatcgatcc agaagctgtt catgtttgat aaaaaaatca acgatctcag
     3721 ctaaaaacaa atagataaaa ataacctctc agcaatttcc aaatgtgagc cttttgctcg
     3781 tatttgaaaa ttgctgtttt ttattgacac ggaaagagaa cgtgtgttct aattgtgttg
     3841 aagaggtgat tgttatgtgg gcgaatcaaa atccggtatt tgtttatgaa aaagaacctt
     3901 caagagatat tttatgtatt gactgcaaat ctttttacgc ctccgtagag tgcgttgaaa
     3961 aggatcttga tccccttaca actaaattag tagtcatgtc ctatccgtcc gattcgacag
     4021 aaacacgggg tagcggatta atcttggctt ccagtcctgc agcaaaaaaa gcttacggca
     4081 tcaccaatat cagccgtgcc agagatcttc ccttccccta tccaagtgat ttatatattg
     4141 ttgccccaag gatggcctat tatatggaaa agaacagaga aatcaattct atctataaaa
     4201 aatatgcaga cgaagcgaac catcatgtct attccgtcga tgaaagcttc cttgatgtaa
     4261 caaatagcat ggcactcttt ggtaaaacaa atgcgtacga catggctaaa ctgatccata
     4321 aagatgtgtt tgagcagacc ggaatctaca cgactatagg aattggcgat aacccgctac
     4381 ttgctaaact agccttagac aatggctcaa aagacggaaa gcaaatgttt gctgaatggc
     4441 gttatgaaga tgtaccagaa aagttatgga aaatctcaga tatcactgac ttttggggaa
     4501 tcggcaaacg aacagcaaaa cgtctgaaac aaatgggtat acattctgtt tatgatttag
     4561 ctcacgcgga ttattatcac ttgaaaagca agctaggggt catcggtact cagctttatg
     4621 cccatgcttg gggcatcgat cgaagttttt tgggacaaga atatacacca aaatcaaaaa
     4681 gtatcggaaa tagtcaaatc ctagatagag attatacaag acgagaagaa atcgagatcg
     4741 ttatcaaaga aatggccgac caagtcggca ctcgcttgag acgatctgca gcaaaaacac
     4801 aagtagtcag cttatgggta ggatactctt taggctatct tgatccaaat ggcaagaccg
     4861 ggtttcacca gcaaatgaaa atcgaagcga caaatgctag cagagatcta gcagatgctt
     4921 tattgaccat tttccaccag cattaccact accaggatat tcggcgtgta ggcgtgaact
     4981 gttccgacct cgtatacgcg acaggtttac agcttaacct ttttgaagat cctgaaaagc
     5041 agataaatga agcgaaagtt gattttgttg tcgatacgat tagaaaaaaa tacggctttc
     5101 aatcaatcat ccatgcgcat agtctcttgc aaggaggacg cgccatcgca cgtagttctc
     5161 tcgttggcgg gcatgcaggt ggtatgagtg gattagaagg tggaaaagat gagacggaca
     5221 gcaaaaaaat tcattgacta taacgagtat cgagatcggc catttggatt aaaatggggg
     5281 actgcttttg cgatggaaga actagtcaac ggtatcaaaa aaaatgaaga agaagcacgc
     5341 aaacagcctc ttcctaaaca acaaatgaca ttgcaagaaa tcgatagtat actgacagaa
     5401 gcctttttat ctcgtaaagc agtaatcatt cagctcaatt taaaagatga atttggaaga
     5461 ctcttggatg aggtcgaagg gcacttcatt ggtgaagcct atgaagatta ctttatgatt
     5521 gaccatcagc cgatattgtg ggaagacgta cggtatattg agttaaagga aacaaaaaaa
     5581 tggtttgtga catgagaggt tgtgacagaa gtgatcaact tcaaaaaata agaaaggatt
     5641 tttcgaaaat tgcttcttaa atttttgtgg aattttggct tatttttcga agaagcaact
     5701 tctggaacaa cgtatattag atatcagagg ttgtgaaaaa gtcgcttagt tttgagtaac
     5761 aagccaaggc gctgatttca caacctctgt ctatctatcc atctattcgg taagattcat
     5821 gcatgacgtc ttctttattt agtatgtaga gccctttttc ttctgaccat tcaacataaa
     5881 tcagctgttt tacatctgca tagccttctt tcttcaagtt ctccataagc catgcttcag
     5941 tcaattgatt ggactgaagc actttatgct gaatttgccc ttcatctaca agcaggatcg
     6001 ataaagcttt gtcttcatct gattttttca atacactcat ttgaccggca ttctctagga
     6061 taacatgttt ggcattttca agaaaaaagc attcttgctg tcttaagatt gctcgcaact
     6121 gctccatctc gatatggtta tttgtcaact ctgtcatgtt gatgacgccg tctttgatta
     6181 gaacagatgg ctcgcctttg acccaacgat tgaccttttc atttttttgg acgatacgtt
     6241 cgataccata aatcattact gcccaaagcg caatggcaaa taggacatgc cctacatgga
     6301 cattgtcatc ataggttgat tcttctaaga tcccacctaa aacaagcgta tagactaaat
     6361 caaacggtgt gatttctgac atggatttct tcccaagcaa acgtaccact aataataaac
     6421 cgatcaaccc gaacaccagt ttcattgtaa ttgaaaaaat cattttttct ccccctaact
     6481 atctgttaat taaattatat atgctttctc acgaacacgc atggaatata caaggaagaa
     6541 atgtatcctt ttactaaaaa aaggactgtg acactgtagt gacccccaaa agttagactt
     6601 ttttggagtg gagaaatcca ctcctttttt ttatgctcta aaatgatatt tttacacaac
     6661 cgttacggtt gttttacgaa a
//
LOCUS       DACEXQ010000149         6669 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.149, whole genome shotgun sequence.
ACCESSION   DACEXQ010000149 DACEXQ010000000
VERSION     DACEXQ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6669
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.149"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(75..605)
                     /locus_tag="IXH04_002043"
     CDS             complement(75..605)
                     /locus_tag="IXH04_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852619.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(704..1054)
                     /locus_tag="IXH04_002044"
     CDS             complement(704..1054)
                     /locus_tag="IXH04_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAQ1852620.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            1256..2386
                     /locus_tag="IXH04_002045"
     CDS             1256..2386
                     /locus_tag="IXH04_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ1852621.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            2478..3233
                     /locus_tag="IXH04_002046"
     CDS             2478..3233
                     /locus_tag="IXH04_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAQ1852622.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSIPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFK
                     LPKAVGKLLGSIQTNLPESVLLDCGMDFLKDIC"
     gene            3291..5330
                     /locus_tag="IXH04_002047"
     CDS             3291..5330
                     /locus_tag="IXH04_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAQ1852623.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSYQATRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(5439..6590)
                     /locus_tag="IXH04_002048"
     CDS             complement(5439..6590)
                     /locus_tag="IXH04_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ1852624.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
BASE COUNT         2293 a         1213 c         1294 g         1869 t
ORIGIN      
        1 aaaaaaacca acaatccaaa agattttttt ggattgttgg tttttatacg gtctatttta
       61 ataactaaaa atgattaacg tatagtcatt tttcttattc tgatgaaatt gataatctcc
      121 tggatgccca tgaaaatcaa aataaagccg aatatttgga atagaagcat caaacttttg
      181 aatggattga acaataagac gaatccgcca atcaacaaca gaatactact ataaacactc
      241 caacttttag cttttgtatt catgctgaag aacaactgga acaagctgtt gatgataaca
      301 gctaatccaa gaagaatcgg caggatagaa acgattgcag gtgcaaaagc atagacgatc
      361 agagctaaga tcaggaaaaa aagaccactg aacagaccga tactccaaga acctgttttc
      421 cttttaaaac ggtaagcctc aaaaagattc aacaacccaa gcaaaacaag ataggcggtg
      481 atcagataac cgataaaatg aaaaacagca gaagggttta gcacaatcgc aatcccagaa
      541 ataatataaa cagcagctct taataaggcg tatttttgaa tattttgttt aatcgtctca
      601 aacatataag aaaacctcct ttgacttatt ttaacgtgaa ctagtcattt taccaaatta
      661 aatgttacaa aatgagcgaa ggagaagaat atctctggaa aaatcaactt ctgaggatct
      721 tttccatttt tttgcctttt gctagctcat cgatcatttt atctaaataa cgaatttctt
      781 tcatcaagtt atcctcgatg tcttctactc gtacaccaca aacaacacct gtaatcaagg
      841 aacgtgaagg attggggaca ggggcttccg caaaaaattg ttcgaaagtc acttgttttt
      901 gtagttgata ttctagttct gtttgggaat aacccgtcag ccaacaaatg atttcatcta
      961 cttcctcttt tatacgatct tttctctccg cttttgctac atacatagga taaacgcttg
     1021 caaaacttgt cgtaaaaata cgattttgcg ccataatcag tcacctttct ttgtatagtg
     1081 ctaaaactcg gacgatgaga tgatgctagg tcagaattct tgttcgaaaa ggtttctgac
     1141 cttttcttta tatttttgtg actgacttat ttgatgagac aaactcgctc ctcacctgac
     1201 aagtggattg ctgtgtgagg aggagatgcg gtgaaaaaag atcgattggt ggattttggg
     1261 tccttatttg actttgtcca tgatcggtct tttagaagta tacagtgcca gttcttatcg
     1321 tttattgcaa gccgatgaaa atacaaaaag tctattattg cgtcaactaa ttttcatatt
     1381 tttgagttgg agcgtgatct tcttagctcg ttcagtcaaa ctacactatt tacttcaccc
     1441 taaaatagca ggatacggtt tagccttatc gattttcttt ttagtattag taagaatagg
     1501 gatattcggt gtcactgtca acggcgcaca acgttggatc tctctgtttg gcattcaatt
     1561 ccagccttct gaactggcaa atcttttttt gattttttat ttaagctggt tttttcgtga
     1621 cggaaatagt agcccaaaag atctaaaaaa accattcctg attacagtag gtataacttt
     1681 tctgatttta tttcagccaa agattgctgg agcattgatg atcctttcga ttgcgtgggt
     1741 catattttgg gcagcggcgg ttccatttaa aaaagggatc tatctaatcg ttactttttc
     1801 tgcattgctg attggagcag caggcggggt attatattta ggaaataaag gttggcttcc
     1861 acagatgttt aatcatgctt atgaaaggat cgcgacttta agagattctt ttatcgatag
     1921 tcatggtgcg ggctatcaaa tgacccattc gttttatgca ctatataatg gtggcatatt
     1981 tgggcgggga ttaggaaata gtatcacaaa aaaagggtat cttccagaga cagaaacaga
     2041 ttttattttt tcgattatta cagaagagct tgggctaatc ggtgcattgt gtgtgctttt
     2101 cttattgttc tcattatgca tgagaatctt ttgtttaagc agtcgctgta aaaatcagca
     2161 agctggattg tttcttttag gattcggaac tttgctattc gttcagacga taatgaacgt
     2221 gggaagtatc gcggggctga tgccaatgac tggtgtgcca ttgccatttg ttagctacgg
     2281 aggaacaagt tatctgatcc tttctttagg aatcggaatc acgttgaata tttcttcaaa
     2341 gatacaggcc gaagagttgg cgctctatcg acctgaaaaa caataaagag aaactgagaa
     2401 actctcagtt tgaattatca taaagtacaa tcttttattt ttcaagcgag aggttctttt
     2461 agaaagagga attcgcaatg aaagtatctc aaaaaatcat attatctatt ttgttactgc
     2521 ttacattagt agttggctat ttttcgattg agtttatcca tggattttcg tctgcaaaac
     2581 agagttcaac tgtgaaaaag gtagagccga aaagtatccc taccacacta aatgtggctt
     2641 tgattggttc agatgctcgt tcgaaagacg aaaacggtcg ctcggattca cttctggttg
     2701 cacaatacaa ccagaaaaca caacaagcaa aactgatctc tatcatgaga gattcatatg
     2761 tcgatattcc aggttacgga atgaataaga taaatgcagc ttactcttat gggggagttg
     2821 atttattaaa tcaaacatta aaggaaaatt tcaaatttga agctccctat tatgcaagta
     2881 tcacatttca agattttatc gattgcgtta atgaactgtt tcctggtgga gtcaagatcg
     2941 atgcagaaaa atctctagat ttagatggcg tatatataaa caaaggagag caagtgatgg
     3001 atggtaatac tttactccag tatgctcgat ttcgggaaga caaagaagga gacttcggga
     3061 ggatcagaag gcagcagcaa gtgatcaaag ctatctctca gcagctgaaa gacgtaactt
     3121 caatattcaa gctacccaaa gcggttggga aattacttgg tagtatccaa acgaaccttc
     3181 cagaaagtgt gcttctggat tgtggtatgg atttcctcaa agacatatgt tagaataaac
     3241 aggtataaat agtgaaataa aggaatgaca agcaagagaa ggaggaaaaa atgaaaagaa
     3301 gtgacaagca cggcaaaaat cgaacaggcg cttatattgc cggcgcagtg atcttaatag
     3361 cagctgcggg tggcggttat ttttactacc agcactatca agaaacccaa gcagtagaag
     3421 ctggagaaaa gacagttgag caatttgtcc aagctttgaa caagggagat tataacaaag
     3481 ctgcagaaat gacatcaaaa aaggcggcaa ataaaagtgc attatctgaa aaagagatct
     3541 tagataaata ccagaatata tacggtgctg ctgatgtcaa aggacttcag atatcgaatc
     3601 taaaagtaga taaaaaagac gattctactt acagtttttc atataaagca aagatgaata
     3661 cctcattagg tgaattgaaa gatctttctt ataaaggaac attagacaga aatgatggtc
     3721 aaaccacgat caactggcaa cccaacttgg tttttccaga aatggaagga aatgacaaag
     3781 taagcctgac cacacaagaa gcagcaagag ggaatattat agatcgaaat ggtgaaccat
     3841 tagcaacaac aggcaaacta aaacaattag gagtcgttcc aagtaaactt ggagatggag
     3901 gcgaaaaaac agccaatatc aaagcgattg cttcctcctt cgacttaaca gaagatgcta
     3961 tcaatcaggc gatttcacaa agctgggtac aacccgatta ctttgtccca ttgaaaatca
     4021 ttgatggagc aacgccagaa cttccagctg gagctaccat tcaagaagta gacggcagat
     4081 attatccttt gggtgaagca gctgctcagc tgattggtta cgtgggagat atcacagcag
     4141 aagatattga taagaatcca gaattaagca gtaatggaaa aatcggtcgc tcaggtttgg
     4201 agatggcttt tgataaggat cttcgtggga caacaggtgg gaaattaagc attacagatg
     4261 cagacggtgt agagaaaaaa gttctgatcg aacatgaagt tcaaaatggc aaagatatca
     4321 aattgacaat cgatgcgaag gcacaaaaaa cagcttttga cagtctagga ggaaaagctg
     4381 gatcaactgt tgcgacaacg ccaaaaaccg gtgatcttct tgcgcttgct agctctccaa
     4441 gctatgatcc aaacaaaatg acaaacggga tctcacaaga agattacaaa gcttatgaag
     4501 aaaatcctga acatccattc atcagccgat ttgcgacagg ttatgctcct ggatctacgt
     4561 ttaaaatgat tacagcagca atcggtctcg acaacggcac tatcgatcca aatgaagtgt
     4621 tgacgatcaa cgggcttaaa tggcaaaaag acagttcttg gggatcttat caagcaacgc
     4681 gtgtcagtag tgatgtatca caagtagact taaaaactgc tttgatctat tccgataata
     4741 tatatgcggc acaagaaacg ttgaaaatgg gtgagaaaaa atttcgtaca ggcttagata
     4801 aattcatttt tggtgaagac cttgatttgc caattagtat gaatccagca caaatttcta
     4861 atgaagatag ctttaactca gatatcttgc tagctgatac tggatatgga cagggcgaac
     4921 ttctaattac tcctatccag caagcagcaa tgtattctgt ttttaccaac aatggcacac
     4981 ttgtctatcc taaattgatt gcagataaag agacaaaaga taagaagaat gtcatcagcg
     5041 aaacagcagt acaaacgatc gtgccagatc tgagagaagt tgtgcaagat gtaaatggta
     5101 cagcacattc tctttctgct ttagggattc cgttggcagc gaaaactggt acagcggaaa
     5161 tcaaagaaaa acaggatgta aaggggcaag agaacagttt cttgtttgct ttcaatcttg
     5221 ataaccaagg ttatatgatg gtcagcatgt tggaaaataa agaagatgat gattcagcaa
     5281 ccaaacgagc atcggaacta ttgcaatacc tcaaccaaaa ttatcaataa aaaattgctt
     5341 tttaagtaca gtaaaaggac tgtggcgtta tcgtcacagt ccttttttca aataaacggt
     5401 tttcacgatg tctctttcac aacttctttt tgatttactc acacttcata aacttttttt
     5461 gtaaaatatt tgagaacaag tggagaaata agtgtactta ataaaatcac gataactaaa
     5521 ggtgaatagt actgtggttc gatcaattga ctttgctgtc caatttgtaa aatgatcaaa
     5581 gccatttctc cacgcgaaat cataccagca ccgaccatca atgaactatg ttgagaaaaa
     5641 ccggcaatct gagcaccaaa ataacctcca agcaatttgg tcaaaatcgc tacaagcgtc
     5701 aatatagaaa taaagagtag ttgttcacta aagtcagaaa aatcaacttc taatcctaca
     5761 ctgacaaaaa atactggtat aaatactgca tatcctaaag cctctacatt gtggtatact
     5821 tcttctttaa cctttgtctg actgacagct atcccagcaa aaaaagcacc aatgacagag
     5881 ctaagcccaa caagatccgc tacgtaagac attcctaaac aaataactag cgatacgatg
     5941 ataatagaag aatttgcgta cactttttct gcaaatgaaa gtaggaaagg cgcaatccat
     6001 ttgactaata agaaaataaa aacaaaataa attagctgtt caatcaaaac aagcggtaaa
     6061 gaagcattgc tagaagcttc ccccgttaga aaagataggc tgaaacttag aactaaaacg
     6121 actaaaatgt catctaccac agaagcaccc aaaattgttg atccttcttt tgtattcact
     6181 acgtttagtt cttttaatac ttctactgaa atactgacag aagttgccgc aagaataatc
     6241 ccaaagaaaa tcgcttcatt tcccgaaact tggaatgctt tgcctgtcaa ccagccaaaa
     6301 acaaccggga agatgatccc aagcaaagca acgaacatac caggtcggaa atattttttc
     6361 aaaagagata aatcactttc cagaccagct aaaaacatta ataagatcac accaatctcc
     6421 gaaaagtcat ggactagaat atcaggtttg acccaattca atccagcttg gcctagcaaa
     6481 acaccaacaa gtaattgtcc aatcactgct gggataccta atctccgcga gatatgtgag
     6541 ccaatcgttg ttgaaatcaa tatcaggcat aaaataccaa taaattccat tctcatcatt
     6601 cctttgtata aaatatagcc cgaacttagc ataacataca ttattgaaaa aataggaaaa
     6661 tatactcac
//
LOCUS       DACEXQ010000150         6625 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.150, whole genome shotgun sequence.
ACCESSION   DACEXQ010000150 DACEXQ010000000
VERSION     DACEXQ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.150"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            614..1582
                     /locus_tag="IXH04_002049"
     CDS             614..1582
                     /locus_tag="IXH04_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1852625.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            1600..2505
                     /locus_tag="IXH04_002050"
     CDS             1600..2505
                     /locus_tag="IXH04_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1852626.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            2502..3308
                     /locus_tag="IXH04_002051"
     CDS             2502..3308
                     /locus_tag="IXH04_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1852627.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            3465..4433
                     /locus_tag="IXH04_002052"
     CDS             3465..4433
                     /locus_tag="IXH04_002052"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAQ1852628.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(4750..5274)
                     /locus_tag="IXH04_002053"
     CDS             complement(4750..5274)
                     /locus_tag="IXH04_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAQ1852629.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            5561..>6625
                     /gene="scm"
                     /locus_tag="IXH04_002054"
     CDS             5561..>6625
                     /gene="scm"
                     /locus_tag="IXH04_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAQ1852630.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT         2087 a         1350 c         1286 g         1902 t
ORIGIN      
        1 aaaaaaccaa aaatagaaca ttctcttgaa accttacttc gtgagcttct acttttgttt
       61 ccttatatta aagccgaaac ggacaatatt tcgtttctgc cttttcttca tcagatagtt
      121 tttgcaactt catatactac acttattttc tataaaagtc aacacttcct ggcgtttctt
      181 ctgctttttc gcaacatttt cttaatgttt tcaattttat gttttcaatt atctgacaat
      241 tcttaattga cagacctttt tatctcggtt atactttaga aaattcattt tgtggaggaa
      301 cttctatgcg aaccaacatc aaccaaatcg caccaacaca actgaataac ttttacttta
      361 gctactttag ataggtttgt attcatcatg atggatacaa acgttaaacg acagtttgta
      421 tctatgatgg ctaaagaact ttgtattgca gcaaaaagcc acgtagacaa aatgctaagt
      481 ggctgagcag tgaaaataca aagtaaaagt atcaagcatt ctactttttc aatttatttt
      541 ccgacttttc aatccatttg gacattttgt ctgaatggat tttttgtttc ccaaaattaa
      601 ggaggaaaaa gaaatgaaaa aaaatataat catcggatta ggcgctgccc tgctactagc
      661 tttaggaggt tgcgcacaaa aagacaattc cgcagaggca tcagatggac aaaaaacaat
      721 tggcgtattg caacttgtac aacataactc gttagatgct gcctataaag gattcaaaga
      781 aggattagca gaagaagggt ataaagaagg cgataatcta acaatcgatt atcaaaatgc
      841 ccaaaacaat caagacaacc tgaaaagtat gagtgagaaa ctcgtcaaag aagatcctga
      901 tttgctactt ggaatcgcca ctccagcagc acaatcttta ttaaatgaaa caaccactat
      961 cccgatgact gtcacagcgg tcacagactt ggaacaagcc catttagtca catccaatga
     1021 aaaacctggc ggcaatgtga ctggcacgac agatatggtg ccaatcgaaa aacaaatcgg
     1081 cttactgtta aatatcgtgc cagacgccaa aacaatcggc atcatgtata acaacggtga
     1141 agcaaactct aagatccaag ctgatttggc agaaaaagaa ttgaaaaaag cgggcgtcaa
     1201 agtcaaagta ctgacagcaa attccacaaa tgatgtccaa caagtgacga aaagtctagc
     1261 taaagatgta gatggaatct atattcctac tgataacacg ttcgcaagtg cggcaagtgt
     1321 tgtcggtgaa gtcgcaaaag aaacaaaaac acctgtcgtt gctggcgcag ttgatcaagt
     1381 caaagacggc ggtctagcaa cagtaggaat cgactacgaa agcttgggca aacaaacagg
     1441 gatcatggca gcaaaaatct tagacggcaa gaatccaagt gaacttcctg tcgaaacagc
     1501 gaatgatttg gaactgtatg tcaataaaga catggccaaa gcattaggca tcaatccaga
     1561 cagcatcaaa gaagctgaat aaaacacagg aggattctca tgacaaattt gctctctctt
     1621 ttcttatcat ctgcatcgca aggggtctta tgggccctac ttgcgattgg tgtatacgtc
     1681 actttccgta tcttggacat tgccgacttg actgctgaag gaagctttcc tttaggtgca
     1741 gggatcgcgg cagtaagtat tacaaatggc tatcatccat tgacagcttg tcttctaggc
     1801 tttttagggg gtgcggcagc agggcttgtt tccggactgc ttcacacaaa attgaaaatt
     1861 cccgcccttc ttgcagggat catcaccatg actggtttat attctgtcac atcccgtgtg
     1921 atgggtgcgc cgaacatctc tttattgggt cagccaacga tctttcattg ggtcgagtca
     1981 ttcggcataa caaaagacaa cgctgtactg atcgtcggtc tgctttttgc tttgatcgtt
     2041 gtatgtttac ttgttttgtt cttacgtaca gaaacaggac ttgctattcg tgcaactggc
     2101 gataatctgg caatgagcga agcaaacggg atcaacacag ataatatgaa gatcatcggt
     2161 tatatgattt ctaatggatg cattgctctt tctggctcct tacttgcgca aaacaacggc
     2221 tttgctgatt taaattctgg tattggaacg atcgtaattg gacttgcttc tattatcatc
     2281 gcagaagttt tgttacgtaa ccagccacta tttctccgtc tcttgactgt cgttttaggt
     2341 gcggtcattt atcgttttat tttagcgtta gtcttcgaac tgaatgttga accaagtgat
     2401 tcgaaacttg cttctgcttt cgtgctagtg atctgtcttt ctttcccgca gatccagcaa
     2461 aaattccatt tacctcaaaa aaagaaaggc ggtagtgtca aatgaatgac gtcttaacat
     2521 tggaaaatct ccatcatacc tttgaaaaag gaacgatcaa cgaaaatcat gtcctgaaag
     2581 gcattaatct ttccttagat cccggcgact tcgtgacgat catcggtgga aatggggctg
     2641 gtaaatctac tttgctgaac agcatcgccg gtacattttc tgtcgatcag ggtcaaattt
     2701 tattgaatgg aaaaaatatc acgaaaaaat ctgttgtcga acgttcgaaa tccatcagtc
     2761 gcgtattcca agatcctaaa ctagggacag ccgttcgttt aacggtagaa gaaaatcttg
     2821 ctcttgctat gaaacgagga aaaaaacgcg gcttcttccg tggtgtcaaa ccacaagacc
     2881 gaacattttt caaagaccag ctggttcggc taaatttggg gttggaaaat cgtttatcct
     2941 cagaaatcgg tttgctgtct ggaggacaac gtcaagctat tacgttattg atggccacat
     3001 tacagcgtcc agaactgatc ttattagatg aacacactgc agcgcttgac cctgctactt
     3061 ccaaaacggt gatggaattg acagaaaagc tgatcactga gcagaatctc actgctttta
     3121 tggtcactca tgacatggaa gacgcgatcc gctatgggag tcgactgatc atgctgcatc
     3181 aaggaaggat cgtcgtagat atccagcaag aggaaaaaca gcagctcaca gtgccccaat
     3241 tattagcgtt gttcaaacaa aacagcggca cggcattaaa agacgatcaa gtacttttat
     3301 cttcataatc agaaagagga tgggacaaaa aatgaacgcc caccctcttc ttttttctct
     3361 ttccttcttt cgtttttgct ttatgtatca accgttgttt cttcgaaaac tattttgttc
     3421 tctccttctt cctgatagaa tggatgggaa aggagcttga tgccatgaca acgaaagaca
     3481 aggtacttca attattgaaa aagacaccag attttctttc cggagaaaag ttggcacagc
     3541 aactggaagt ttcccggaca agtatttgga aagccatcaa agaattggaa aaggcaggct
     3601 atcgttttga gcacgggcct acagggtatc gttacctgcc ctctgacgta ctagatgcaa
     3661 aagaaatcca gcaagagcta cggtcgttta ttcctgattt gaaagtacaa acgatgttga
     3721 cttctgaatc tacgatgaaa gatgccaaac tggcagcagc tcaaggagat tctgtttcga
     3781 agttatttgt tgctgataca caagaacaag ctcgtggacg ttttggacgg ccttttttcg
     3841 ctgaacctgg acgaggaatc tacatgagtc tcttgctcca tccagacaaa cggtttgatg
     3901 aattgcctca atatacgatc atcgcagcca gtgcctgtgt gaaagccatt gaatctgtaa
     3961 ccggtcgttc tgcttcgatc aaatgggtca atgatatcta catggatgga aaaaaagtct
     4021 gtgggatttt atctgaagca gtcagtgata tggaaagcgg caggatcagt catatcatca
     4081 ttgggatcgg gcttaatttt tctgttcctc aaaatcattt tccagctgat ctccaagaaa
     4141 aagccatctc tgtctttccc gacggacaag ctagtgtcac aaggaatcag ctaatcagca
     4201 aaatctggca agaattgttc acttatcttt ctcacccaga aaaagcgttg gacttctata
     4261 aagaaaaatc attcgtattg ggaaaaaccg tgtcgttcaa tcaaaaagga acgacttatg
     4321 tcggtcttgc aaaagaaatc ggtgatcatg gcgaactgat cgtccaaaca aatgaacaga
     4381 agatgagttt gtcttctggc gaaatcagtc tcacatctat ccgtacgcaa taattatcag
     4441 atttcacaaa aggacacctg atctcataca gtgtcctttt ttctgaattc tcttcaaaag
     4501 actctcctta ttctatcagc tgacagtttc tctatcctct agaaaaaggt attctaaaaa
     4561 tatctataaa gcatggtaca ctttctttaa atgaatcaat gcaaaaaaca agagagtcat
     4621 cagtttacac gatcaaaaga gatagcaggc cgcttccttt ttcaatctta tagatagacc
     4681 atgggtaacg ataaaaacaa aaaagaactt gcgacttatt ttgccgtaag ttctttttta
     4741 gctagtgcct taatctctta atcccaacaa acgtctcgca tagatcacct gaccagagtg
     4801 catagcagca tcatcaataa tagaaacgag acgcattcct cgtgtgactg gtggtgtcca
     4861 gttctcgtca attacttcat ctagctcgga aggttccagc cttcttaagt aagaaatagc
     4921 aaaatcagca gctcctcgca agtatcctag cagatcttct atttgattag cttggatctg
     4981 ctttgcttgt tccagactat gcttccaatc ttgttcgtta tctgctatcg tcatcggaaa
     5041 ccggctttcc cagtcttgac tatgccaaat tggagaagtt cctgctaaat cagcaatctg
     5101 gaaatctaat tctcgagcgg tatgccaagc cagccaagtc attgatttga tctgcggggc
     5161 tctatcttcc actggaaaac gattggcctc ctcaatagag acgccttcaa acaaacgttc
     5221 aaaccgatcg tatgcccgat ccacactctc gcatagcata tctaaataat ccatttattt
     5281 tcctcctttt tatttatcct acttactttt tcctaattct aggataccta cttatatgtt
     5341 actacacacc cttatttttt acactttaac agtcaccatc atcaatagtt tggagattga
     5401 aagaaaaaga tagtgtcgaa ctatcaattg aacaagaagc aacaatatga acgtttcatt
     5461 aatattcttt aaataaacaa tgatttttca aacgaacagt ttaagactag gagaaaaaga
     5521 gtataatagg aaaatgtttg aaaggaaagg aaggggaaaa atgaaaaaaa gggcaagttt
     5581 actagtccta gttgctttgt tgttcaatat cttttcgtat ggtgtggccg tttcagcgga
     5641 aacatacatt cctaaagacc ctgggaatat ttataatgat ctaactggta actatggctt
     5701 gttaggaatt gctagtcaat tccacgtatt tgctaaaaat aagacaactg tgaatgcaca
     5761 tacagacgga aatatagcaa cacatgaact tgatgcaaaa aataattttg gtacagagct
     5821 tcatgaaggt ttattgaacc aagaaataaa ttatatacaa aaaactgatt ctttcgctaa
     5881 ttcttcaggc atacctacag gtagcacaag aatgaataaa tttgtcgttg gcagtaacgt
     5941 ggatgtagca cttcaagatg gtcatcctac agtcaatgga tccaggcttg atcacttaac
     6001 tccagatgat ttttataaag atcgtgctgg gcatgaatat attgatttcg agcaggaatt
     6061 tgctaaattg aatgtagcag caaatgatct tgcaacgatc actccagcta aaacatatac
     6121 tgctgcagat tttccagaca tgaacaatcg gacaatcgat ttaactgatt tatcagatac
     6181 cgggttttta ttggtaaata tcgatgcaga agtgttaacg atgaatacgc cacttcaaat
     6241 cattaatcca aatgatcaag tagttgtatt taatgtcatc aacagttctt ctgcattgaa
     6301 cgtacagtca ccaatcaaat acaatgatcg ttcaaatcat gaaacagaag atttttcgga
     6361 tgcgaatatc tcttggaatt ttggaaatga gatgactgat ttgacaatca gtgctccttt
     6421 tcaaggaaca atccttgcgc ctaatgctac tatccgcatt actcaaaacc aagacggaac
     6481 gattatagga acaaatgtga ttttagatgc agctaccaat cgttgggatc ctaacgaaat
     6541 atttatcagc gacaatggta cagatacgac agacacaagc gacagtacag atacatctac
     6601 cacaagtgat agctcagatt catca
//
LOCUS       DACEXQ010000151         6616 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.151, whole genome shotgun sequence.
ACCESSION   DACEXQ010000151 DACEXQ010000000
VERSION     DACEXQ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.151"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(189..965)
                     /locus_tag="IXH04_002055"
     CDS             complement(189..965)
                     /locus_tag="IXH04_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1852631.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(1213..2049)
                     /locus_tag="IXH04_002056"
     CDS             complement(1213..2049)
                     /locus_tag="IXH04_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAQ1852632.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(2060..2989)
                     /locus_tag="IXH04_002057"
     CDS             complement(2060..2989)
                     /locus_tag="IXH04_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAQ1852633.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            3181..3534
                     /locus_tag="IXH04_002058"
     CDS             3181..3534
                     /locus_tag="IXH04_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ1852634.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(3608..4264)
                     /locus_tag="IXH04_002059"
     CDS             complement(3608..4264)
                     /locus_tag="IXH04_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ1852635.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            4490..6424
                     /locus_tag="IXH04_002060"
     CDS             4490..6424
                     /locus_tag="IXH04_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1852636.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
BASE COUNT         2136 a         1274 c         1150 g         2056 t
ORIGIN      
        1 tgaattcaat catttctgta ctccttttgt ttgttattac tttttattgt acccttcgtt
       61 attggtggag taaatgtcaa aaagagtgca taacaaaaaa ggatagaaac gactaggctg
      121 ctacttgggt tgttctcttc tacaggaaaa cactcttcta acaggtaaga tatgagtctt
      181 tgttttcttt aaaatgattc attttctatc ttttcttgca taaaattctt tagtttatca
      241 atacttctct ttcccatctc tagttgttct ccatttgcaa atgttactat ttcgtttact
      301 ctgctaatag atgtaatgtt ttcagtatta atgatataag cttttaaatc tttacataaa
      361 tactcatatt cgagtgattt tttatatttt ttaattgtgc cgtttacgag aatctcttca
      421 tctttcaata ctaggcctat tttattttta taacctggca tgctcattag ataaaggatt
      481 tcatcgtaat ggatatagtg atcgctatta ccataacgga cagttgtcca cctttttttc
      541 gtagcatgtt gaacaggata gttttctttt tctttcaata agtaactttg gatgcttttt
      601 ttattttttt cataagtgaa ttcttttatc aaataaccac taggcgtaat ctttcgatta
      661 ataacagtta ttgctttatt cggatgagac gttagataaa caattttaca cttttcattc
      721 ttttctctaa gcatttcgcc tagttcgata ccgctataat aggcgttcaa atcgatgtct
      781 aggaaaaaaa tatctgagga atagatttca ttatttttaa tgaattcaac aaattgtata
      841 acgttcaatc cctgaacgat ctcaaatttc caaggactta ataaaattct ggattcttcc
      901 aaaatttcta ctagcatttt ttcaaaaacc aaactatcat ccaaaataaa tatacgcgtt
      961 accataaaaa gtacctccac tcgatatttt actatcaaaa tcatttcata caacttttca
     1021 acatacatgt taggaataaa ttgtaattct cttcacaatc ttttccaaca cctttgaaac
     1081 cgatatcaaa tactttcagg atgctgcttc taaaataaat atcaaacaaa tgaatacatc
     1141 atgcaaaaaa agccagacaa ccgacgtcct tgcgtgtcag ctgtctgagc tatgtttatt
     1201 tattttgttc ttttaaaaag aacgatacac atactgattt ttcggagcag agactttctc
     1261 agcgtttgta acaactacag atgggatttc acgcttgaat ggttgataat agtctgattg
     1321 gatggttcct tctattttat accattcatc atttttaacc gttactcctt ccggcatatg
     1381 cgtcaacaaa ccaaagactc ctgaatctgc cacacaatgg ataatgccga atcggaacaa
     1441 gaagatatct gtattgctat cgttcggtgt ttggtaaaca aatccttcat agctgatttt
     1501 tttgccaatg aattcgctag ggtaattata aattagctcc ataacttcta aatagttttc
     1561 atctgtgatc ttgatagttt gcttatcccc atacttttct ttcaactcat tcatttggtc
     1621 taagtagtct gatttgttga aataaatact ggtatccggc tttaagtatt gtgtctgcat
     1681 ctccggatcc cctactgatt ctttacttac tgggaaatta aatcctttgg cttctacgat
     1741 cgtagtgtcg agactaactg ttggaaacaa cgtccccacg attactggca aagctagcag
     1801 tacataagcg attccacgtt gataaggttt tgacaaccca tggtcatgat cgtgctcgtg
     1861 tgccttttct tctgcttttc cgtttttgac ccacaaatac agctgaacta aagccaaaag
     1921 agcagataaa atcattgaaa gaatagctaa gtagcgataa tggacattga tatattgatc
     1981 gagtttgcca gaaacttgca agtacatcat caattcgaaa tacccagata aaatcaaaaa
     2041 ccgcagcata ttttccctcc taaataaata atgtgaatcc aataacgcta agggcaatca
     2101 gtccgatcag cgataaaata aagcgtcctg taaaatatcg tttcatcatc agtagatttt
     2161 tgatatcgac cattgggcca aagaccaaaa aggcagctac aggtgctgtg ccgaacagac
     2221 ttaacaaaga agatcctata aacgcatctg cttctgagca aagagacatc gtaaaggcca
     2281 ataacagcat gatgataatg gctaaaatct ttgttgagcc gagcgttagc ataattctag
     2341 ttggtaaata agtctgcatc gcagctgcga ttaatccgcc aatgatcaaa taacgacctg
     2401 tatcaaaaaa ttcgtcgatt gaatgtgtca acacatgccc agtttgatgg atcagctgat
     2461 tttttggttc attcttctca ctttgttctt cttcatggac atgatgatgg tgattttctg
     2521 ctactttaga cgtgattttt tctttcaata cagcgtcttt ttgaaaatag gcaagccaga
     2581 caccaatcac aagggcaaca aaaaagctgc ctatcacccg ccagcttgcc atctgccaag
     2641 aattgccaaa tgcgataaaa gtggaaaaaa tcacgattgg attgatgatc ggagcagtca
     2701 gcataaaagc aaacgctgtg tgaactggta cgcctttttt aacaaattga tgaacgatcg
     2761 gtacgatgcc gcattcacat gaaggaaaga aaaatcctaa gatcgaacca gtaaaaatag
     2821 agaagatacg attttcagga agccatctct tcacacgttc tggggtcaaa aatacttgta
     2881 aggcgcctga gataagacag cctaataata cgaaaggtaa tgcttcaata acgatcgata
     2941 agaaaatcgt actcatctgc aaaaccgaat ttggaataaa agaaaacatg agtttcctcc
     3001 tttttatttc tagttcattc gctttgcaaa tatacgcttt tattcaaaaa aaacaagttg
     3061 ctctgtaaat ttttacacaa ctcttatttt ttaccaaagc acttcaattg actttttctg
     3121 ttattcccat taaattaagg tctcatacat atcacttcta tcataaaagg gagaataaaa
     3181 atgaaacaaa ccactaactt gactgaagtt caagacatac tgcaacagtc atccgttgcc
     3241 gtcatttatc tttcgatgcc taattgcagt gtttgtcacg ctgttcgtcc acgactagag
     3301 gaacttctca cacattatga tattccggca cttcatttgg atgcatttga aacaccagaa
     3361 gtagctagta aatttgaagt gctgactgcg ccagttgtcc tgatttttca tcaaggaaaa
     3421 gaagtctttc ggcaagcacg gttcatcgac tttaaaaaaa tccgttatct actagatgaa
     3481 ctgacagcag aagatgattc cctttcttat gaagaaatct tccgaacaaa ataatcgttg
     3541 tccaaacaga aaaaagcgat tgaacaagtg aaagttttgc cttcatttgt tcaatcgctt
     3601 tttattttta ttctgctgta ttttcggaat gcgcatttgc gtcagaatgc aagaaataga
     3661 tcaaggtttg caattcattc gtcaaatcaa cattttggat caatacatct gacggagcat
     3721 tcaatcgagc agcggtaaag ttcaagattc ctttgattcc tgcttccgtt aactgattga
     3781 ctacttcttg tgctctttca gcgggtatcg ttaaaattgc tacttcgatt tgttggatct
     3841 tgatttgttc tgacatatct tccattggat aaacggggat accgtctacg atgcgcccaa
     3901 cgatttcctc attcacatca aaagcacaac tgatacgtat gctattactt tgatggaatt
     3961 tgtattttag taaagcactt cccaagttcc ctacaccgat cagcgcaaca tttgtcaatt
     4021 gatcgtcgtt taatgtcttt gcaaaaaagt gcataagatc ttctacatca taaccatacc
     4081 cacgttttcc taattcgcca aaataagaaa aatctcgacg gatcgttgca ctatctactt
     4141 gaacggcttc acttaattca gttgaagaca cttttttctt tccagcatta tttaaaatcc
     4201 ttaaatagcg ataatacaaa ggcagtcttt tagctgtcgc ctttggaata atatgatctt
     4261 tcacgaaatg acctccacgt attgtctaat tacatctttg atttctccat catagcagta
     4321 tttataggaa aaaatcaatt ccattgctca gatattgtga aattttattc tttattcatt
     4381 tatttatcat ttctgcaaat ctcgcagtat aatggaattt atggtagact actaattgaa
     4441 aaatagtttt ttatttgagg agaattcaca aagtagaaaa gaggattcca tgatattatt
     4501 acaagccaat cagattgctc gccattttgg tgcagaagtc ttatttgaaa acatacattt
     4561 agaaatcagt acaggcgctc gtgtcgcttt agttggcaga aatggcgctg gaaaatcgac
     4621 tttattaaag atcattgctg gaattgaagc acctgatgaa ggaaagatcg ctaaaaataa
     4681 aacagcgact ctcggatacc tcgctcagga cacaggactt gattccaatg aaacagtttg
     4741 gaacgagatg ctgaaagctt ttgaacaagt tagacaaatg gaagagcgga tgcgcgagct
     4801 agagattttc attagcgaag gtacgccaga cacacctgct tatagttctt atttaaaaga
     4861 atacgaccgg ctgcaacacg atttttcaga tcgaaacggc tatggttatg aaaatgaaat
     4921 tcgttccgta ttgcatgggt ttaaattcga ttcttcattt tatgatcaga aaatcgatac
     4981 tttatcaggc ggacagcgca cacgtttagc tttagctcgc atgcttttgc aacaccctga
     5041 cattttgatt ctagatgaac caacgaacca tttagatatc gagacacttt cctggcttga
     5101 gaattatctg caaagctata ctggtgcatt attgattgtt tcccatgacc gttatttttt
     5161 agataaagta gtcaatgaag tctatgaatt aagcagaaga aaaatgcgcc attacaaagg
     5221 taactattcg aaatatctag atttgaaagc agaacagtta gcaagtgaat ggaaggctta
     5281 tgaaaagcag caaacagaaa tccataaatt agaagacttc gttgctcgga accttgttcg
     5341 cgcctctacc acaaaacgag cgcaaagccg cagaaagaca ttagaaaaaa tggaccggct
     5401 ggatcgtcct caaggggatg aaaaacaagc aaactttcta ttcagtatag aaaagccttc
     5461 tggaaatgta gtcttgcaaa tcgaagatgc cgctatcgga tatgataatg tcaccttatc
     5521 tgaaccaatc aatctagata tccgtcgtca agaagcgatt gctctagtag gtccgaatgg
     5581 tatcgggaaa tcgacgttgc tgaaatcaat cattcaccag atccctttta tccgtggaaa
     5641 agaaacgcta gggacaaatg tcaccatcgg ctattacgat caaggtcaag ctgatctgca
     5701 tacaaacaag acgatcctag cagaattgtg ggatgagcat cctaccactc cagaaaaaga
     5761 gatccgaagt gctttaggtg ctttcttatt ttctggcgaa gatgtagaaa aaccaatccc
     5821 tctgctaagt ggcggtgaaa aagcacgggt tgctttagcc aagttagcga tgaacaaaga
     5881 aaattttttg attttggatg aaccaacaaa ccatttagat attgacaaca aagaagtatt
     5941 agaaaatgca ttgatcgatt atgacggcac actgcttttt gtttcccatg accgatattt
     6001 catcaatcgg atcgcgacaa aagtcgtcga actctctgaa aatggcagca aagtctatct
     6061 aggagactat gattattatt tagagaaaaa gcaggaagaa gaagaaatcg ccgaattgtt
     6121 ggcacaagat gagccagaat ctgctcctgc tcccacgaac aaatctgatt attaccaaag
     6181 caaagaacag caaaagctta tccgtagcct tcaacgaaaa attactcagg ttgaagaaga
     6241 aatggcgcgg atcgatgaat ggatcgatca gttggaacaa gaaatggtcg cgccagaaaa
     6301 cctggaagat cacgtaaaac tcaatgagct gaaccaagat ttagaagctg ctagacaaga
     6361 tcaagaaaca aaattgacgg aatgggaaga actaagcttg gaattagaag aatatgaaaa
     6421 ttaacgaaaa aaccaaaaca gaaaccattc atgctgtttt ggtttttctt tttttatttt
     6481 atcaaacaaa aattccatat ttaccttttt caccaataag aagaccaatt actagtcatt
     6541 ctcactaaag cttctaaata atagtaatca ccccaaatac aaggtgtatt taccccatta
     6601 tttttgggct tattat
//
LOCUS       DACEXQ010000152         6521 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.152, whole genome shotgun sequence.
ACCESSION   DACEXQ010000152 DACEXQ010000000
VERSION     DACEXQ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6521
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.152"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(28..726)
                     /locus_tag="IXH04_002061"
     CDS             complement(28..726)
                     /locus_tag="IXH04_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAQ1852637.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(719..2071)
                     /locus_tag="IXH04_002062"
     CDS             complement(719..2071)
                     /locus_tag="IXH04_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAQ1852638.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2294..3694)
                     /locus_tag="IXH04_002063"
     CDS             complement(2294..3694)
                     /locus_tag="IXH04_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAQ1852639.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3855..4592
                     /locus_tag="IXH04_002064"
     CDS             3855..4592
                     /locus_tag="IXH04_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAQ1852640.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4717..5196
                     /locus_tag="IXH04_002065"
     CDS             4717..5196
                     /locus_tag="IXH04_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAQ1852641.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5193..5960
                     /locus_tag="IXH04_002066"
     CDS             5193..5960
                     /locus_tag="IXH04_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852642.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6190..>6521
                     /gene="lepB"
                     /locus_tag="IXH04_002067"
     CDS             6190..>6521
                     /gene="lepB"
                     /locus_tag="IXH04_002067"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ1852643.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFS"
BASE COUNT         2218 a         1239 c         1206 g         1858 t
ORIGIN      
        1 tttgtcacat tccccttttt cgaataacta ggctgtttgt ccttcaagat agggcagatc
       61 caattcttta ccgtaacgtt gttctaaagc taggcggatc aaacgagctg ccaggttctc
      121 atttcttgca aggataggtc catggaaata agaaccgaag acatttcggt aaatgacacc
      181 ttcagattgg tcttcaccat tatttccttg accttgtacc actttcccca aaggtcgttc
      241 accttcacct aagaaagtcc gaccattgtg gttttcaaat ccataataag tttcattgaa
      301 ttcttcgtta tggataacga tatcaccgat aaaccggtta ttttcctgac ttaacgtgta
      361 gtgatccagt gcactgattc cttggatctt ttctccatta gctcccatgt agtagtgacc
      421 tagcaattgg tagccaccac aaatggctaa catgacgcca tcattttcga tatagttgat
      481 caaagaatct ttctttttct ggatgtcttt tgaaatgatt aattgctcaa agtcttgacc
      541 accgccaaag aaaaccaagt catatttatc agcatcaaaa ggttcatgta tactaacgat
      601 ctccgtatgg caagaaaccc ccattttttc tgctatatat ttcaacatca aaaggtttcc
      661 gttatcacca tatgtgttca tcaaatttcc ataaaggtgg gcgatattga gttcataatt
      721 agccacggtt cattcctcct ttgataaatc cttgagcagc tagttcttta cgaagctgca
      781 atacagcagt gtaagtagct aaaatataga catgctcagt tggcaattct ttgatttttt
      841 cgattacttc tggtaattct tttgtttctg tcaatttatt ttctggaatc ccggcaactt
      901 ttagacgtaa agtcatatct gtatgacggt ctcctccaga aataacagct gggatatcca
      961 tatcggcaaa gttttcaaaa ttaccatccc aaatccagct gacatcgatt ccgtctgcgt
     1021 aattggcatt taataaagcc actaaagaaa atggataagg agctagacca atcatatcga
     1081 ttacttgatt gatacctaca ggatttttga caagtactaa tgtacatttt ttcccattga
     1141 tattgatgac ttcttgtcgg ccgaatactt tctcgtcata tcctaaacct tgtttgattt
     1201 tatcaggatc gacattgaag tactctgcca ctgcagtagc tgctaaagca ttgtagacat
     1261 tatacatacc gccaacttcg attccgtatt catgaccatc gatcacgaaa tctgctgaca
     1321 cattgtccat ccgtaccata tcagttaatc gataatccaa ttcaggacgt ttgaagtcac
     1381 aatttggaca gtaatatttc cctaagtttg cataagtgat cattttataa tgcaagatgt
     1441 gatgacaatt tggacataag acaccgtctg tattgtaatg agccatttgt tcacgatctt
     1501 cttcatggtc aaagccataa tattttctag gattgactgt ttcgatagaa ttgaagattg
     1561 gtgaatcacc gttactgata atcgttgcat ttggtgactt tgcggcaccg tctacaatca
     1621 ttttataggt cgtatagatt tctccatacc gatccatttg atcacggaaa atatttgtaa
     1681 ataagaacag ttctggtttc atatattctg tgactttgct caaacttgct tcatctattt
     1741 ctaggacagc aaactttttc tgtccttttt ttggttttgc ctgcaagaaa gtcgatacaa
     1801 ttccctgtac catattcgca ccagtagggt tggttagaac ttcatcgaat tgctggcgca
     1861 aaatattgac tgttaaagcg gtagtcaatg ttttaccgtt tgttccggtg acaacgacga
     1921 cttggtagtc ttttgccaaa ctatctagta tatgtggatc gactttcagc gctagtttac
     1981 ctggtaaact tgatccgcct ttaaaaaaag tttttaatgc ccattgcgac gtttttccaa
     2041 ctgcaatcgc aaaatgactg cgtattccca ttatgtaacc tccaaaaacg atttgctgac
     2101 gaataaattc aactaatcat accatatgtt gaaaaaaagg ttaaattgct tttcttattc
     2161 ttaagaaaag gttacggctg aaagtgcatg aaatcggaca aaatcgcatg atttaacaaa
     2221 aaaacagctt gaaacaaaat gattgtttca agctgctgtg attttattat tccctcttac
     2281 ctatattccc attttaaccg ataataattt tctcgcttgg atattcatag cctagatctc
     2341 gtgtttcttt tggcacgaat aacatcaatg tatatagcat tccgatccgc ccaataaaca
     2401 tcagtgtagc aatcgtgatt ttgcccacaa cagacaagtc tccggtaatc cctaaagata
     2461 aaccagttgt tccaaacgca gaagttactt cgatgatgat cgagatcagc gtttgttctt
     2521 ctgtagcaga taaaaaaacg atacaaaaga aacacatacc aagagaaagc ataaagacaa
     2581 cgactgattt tcgcacatca tcttgatcga ttctccgtcc aaaaatattg atgttatctt
     2641 cacttttgag gaaggaatat agatacaaac cgatgattgc tacagtagtt gttcgaatcc
     2701 cgccgcctac agaactagga ctgcatccga taaacatcag taaagaaaaa atgatcagtg
     2761 ttgtgatttg aaaatcaccc aaatcgtgaa tctgcagacc agcattacga gtcgtaatcg
     2821 aataaaacat cgaattgatc catttgacac tcatattgga atcttggaat aaatggtcct
     2881 tttccaatag ataaatcaag acagttcccg aaacaaacaa gataacaaaa gctaacacag
     2941 ctaatttggt aaacaaggaa aaacgaaacg gaagcttcgc attggaacgt ttgtataata
     3001 gccattcccg gcactccatc aatacaggaa aaccaattcc tccgacaaaa atcaaaaaca
     3061 tgatcaggat caagaaaaaa tagtcatgtg caaagggctt gatggaatcc ccagtgacgt
     3121 caaacccaga gttcgtgaca gccgatatcg cctgataaaa accgtaaaaa acggcatcac
     3181 gccaacgatc aaaatagcct ctgtaataaa aataaataga aaagaatgtc ccaaaaagta
     3241 attgaaacca gattaaaata gcaaaagtga tccgaatcag tcggacgata ccagacaaac
     3301 gcggttgatt catatccgtc atgatcagct gtcgctgttt cagtgtgatc cgtcgtttag
     3361 aaaaaatgac aaaagcagta gaaatcatca tgatccccaa accaccaact tgaaataata
     3421 tttccagcag tataataccg ttatcattga acacagaatt gatatcaaaa gtcgatagtc
     3481 cagtcacact cactgtactg attgccatga acagcatgtc cacaaaagaa acatggctat
     3541 ctggttcgcg aaaaatcggc atataaaata gaacgagtga taagaccgtc atcaatatat
     3601 aataaaaaac gatgatttgg atcgatgaga aatgattggc agcaaacttc tgtcctcgcc
     3661 gctgcagcct tcgaaagaat aaatcaacat gcacagtact atcctcctga caatagtcgt
     3721 ttctaccagt atacaagggg aattaggaat actcaaagaa attatcaaac agataacaat
     3781 acagctttcg attaaaatag aaatggctta taataaggaa aatcctagaa aaaagaagga
     3841 ggaggaataa caaattgatt aatgctcaac aaatcgtaga aaaaatgaaa aatcaaaaaa
     3901 taaattatga tacagttctg aaaaaaatga tcatgcaatg ggagaaagaa gaagtaaagc
     3961 ctaagatatt aatacataca tgctgtgctc cttgcagtac gcacacattg gaattcatgt
     4021 ctcaatatgc agagatcacg ttattttttt caaattcgaa tattcatcct aaaagtgagt
     4081 atttgcgaag attacatgaa caaaaacgat ttgtagacca atttaatgag aaaactgacc
     4141 atcatgttgc gctaatcgtt gatgaatacc gtccaaatga attcaccaaa atggttttaa
     4201 caaaccatct tgaaaacgaa aaagaaggtg gtgctcgctg cagtgcatgc ttcaatatgc
     4261 gattagatct tgttgcgcaa aaagcgcaag aactaggata cgattacttt ggaagtgccc
     4321 tgaccatctc gccaaagaaa aatgcagctt tgatcaatca gatcggaatg gatatccaaa
     4381 agatttacgg gacacaatac ttgccaagtg atttcaagaa aaacaaaggc tatgaacgct
     4441 ctttgcaaat gtgcaaagag tatgatattt atcgccaatg ttattgtggt tgcgtgttcg
     4501 ctgccaagca gcaaggatgc gatctgaaag aaatcaatcg ggaagcaaaa gcttacttaa
     4561 aaacatcaac cgtagttttt gaggaaaatt aaagaaatct ttcggaaaaa cgtaagaaaa
     4621 aaataaagaa tttatggtag aaataaggta gactgtataa tttgtccgta gtaaagtaag
     4681 gaaacaagaa taaatttcaa tgaaggaaga gataagatgc gaaaagtagc acctattttg
     4741 aagccaatcg ctgtattaac aatctcgacg attgtcgcga ctactacagg tattgtgaat
     4801 agttccagct accgtgcacc aagttcaagt tctagtactg tagaagaaac aacaccaact
     4861 acttcagtct cgacatctga accaacaaca acggaaagtt tgaatgaaac cactacgaca
     4921 agtacatggg acgatacaac aactagttcg tcgtatgatg agacatatac aactgacagc
     4981 tcaagcgaaa caagtaatac ggtaccagat ggcactccat cggattcaac caatccaagt
     5041 acaagtccgg atacaagtgc tagctccgaa acgataccaa gcagtacgac tgtagatgat
     5101 aacacgcaaa atgatgatgc agcacgtaca gcacagcaaa accaagattc aggaaatacc
     5161 caaacgtata accagcaagc aggtgattct caatgacatt tttactagac cttatcaacg
     5221 gggtgcataa attccttggc tatttagaca tcagtcctaa atatcttaac cgtggatata
     5281 cgattttaag tgtgattccc acgctttatt tattgagaat tgtttatgga ctttggcaaa
     5341 accaaaacta tcttcaattt ttcttatatg gacttgcatt tctagtgctt gtttatttta
     5401 ctgttttaaa tgtattttat tactttttag ataaaaacac gaaagccgat gtcactcagc
     5461 tttttgtgaa gtatttacct gatgaagcgt tcaacatcca gacagaagaa tcgatagata
     5521 atggaaattc gatcgataaa gtgaatacac aagaggtcat ggtttcttat gacgaagatt
     5581 atcaattgaa attagccgag aatatgcggt atttgattgg aaatggtgag atcaaaacaa
     5641 atgaactcgg tcgcatggat ggatttctgg tcgatagaaa tacattgtat ccttattact
     5701 atgtcaagaa aacaggagag aaagagtatg ttctaaatat tggacgaagc tactcggaca
     5761 tggaaaaagt agggaccatt cgacttgact caacagaaga cacattggaa cctgtcggat
     5821 tgttcatcgt cggcggtgat tttgtcaaag acgggttacg ctatcatgag ccttatcgtc
     5881 tgaaattgat tgcaaaaaag gcacagtcgc aggaagaacc agtggtttat agtcgtagcc
     5941 aaagaagaag acattcataa tgtgatgaaa cgccgtgtat cagtggttgc gataaaaacg
     6001 tttattcgga ataaggggga tgtgacacgt cttttgtcac gttccttttt tatataacga
     6061 gaaagataag acgaacacat cacttattct ttcctttcat ttataaacat tgtagaataa
     6121 agaaagtaca acgaagtatt gtgaaaaaac gtaataaata acgaggaaaa tacaagtagg
     6181 aggtaagctg tgaaagaatt tttgaaaaat tgggggattt tgatccttgt attagcggct
     6241 attttattag ctagagtgta cgtgtttaca ccagttactg taaacgggca ttcgatggac
     6301 cctacattaa gtgatggaca acgattgatt tcatctaaga tctcaaacta tgaacgaatg
     6361 gatattatta ctacaaagga accgggggat gaagaacgga tgattgttaa acggatcatc
     6421 ggtatgcctg gagatacagt caagatggaa aatgaccagt tgaccattaa tggcaaaaag
     6481 tatgatgaac cttatctgga tgagttcaaa aaagaatttt c
//
LOCUS       DACEXQ010000153         6517 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.153, whole genome shotgun sequence.
ACCESSION   DACEXQ010000153 DACEXQ010000000
VERSION     DACEXQ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.153"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..699
                     /locus_tag="IXH04_002068"
     CDS             <1..699
                     /locus_tag="IXH04_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1852644.1"
                     /translation="ALKDLSLSIRQGEIFGFLGHNGAGKSTTIKSLVSIIEPSSGTIL
                     VDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAYELDTQKKEKRLAELT
                     ALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPLQGLDPQAAFDLKEMM
                     KAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSELLNGSPDETLEKIYL
                     KMAGRQATGDGPYV"
     gene            692..2272
                     /locus_tag="IXH04_002069"
     CDS             692..2272
                     /locus_tag="IXH04_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAQ1852645.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(2523..3002)
                     /locus_tag="IXH04_002070"
     CDS             complement(2523..3002)
                     /locus_tag="IXH04_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAQ1852646.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(3018..3770)
                     /locus_tag="IXH04_002071"
     CDS             complement(3018..3770)
                     /locus_tag="IXH04_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAQ1852647.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            3887..3957
                     /locus_tag="IXH04_002072"
     tRNA            3887..3957
                     /locus_tag="IXH04_002072"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3919..3921,aa:Gly,seq:ccc)
     gene            4318..5043
                     /locus_tag="IXH04_002073"
     CDS             4318..5043
                     /locus_tag="IXH04_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAQ1852648.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            5298..5564
                     /locus_tag="IXH04_002074"
     CDS             5298..5564
                     /locus_tag="IXH04_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAQ1852649.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            5561..5992
                     /gene="ruvX"
                     /locus_tag="IXH04_002075"
     CDS             5561..5992
                     /gene="ruvX"
                     /locus_tag="IXH04_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAQ1852650.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            6017..6322
                     /locus_tag="IXH04_002076"
     CDS             6017..6322
                     /locus_tag="IXH04_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAQ1852651.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
BASE COUNT         2113 a         1138 c         1291 g         1975 t
ORIGIN      
        1 gcattaaaag acttgtctct ctcgataaga caaggggaaa tttttggatt tttaggacat
       61 aatggtgccg gaaaatcaac tacgattaaa agtttagtta gtattattga accatcatcg
      121 ggaacgattt tagtagatgg aatgaaattg acggaaaatc gattgagtat caaacaaaaa
      181 atcggttatg tgcctgattc cccagatatt tttttacaat tgacggctgg agaatattgg
      241 gacttgattt cagctgccta tgaattagac acacaaaaaa aagaaaagcg tttagcagaa
      301 ttgacagcgc tttttgatat gtatagtcat caaaatgaaa caatcgcaag tttttcacat
      361 ggaatgcgtc aaaaaacgat tttgatcggc acgttgctgc cagatccaga tatttgggta
      421 ttggatgaac ctttgcaagg acttgatcct caagcagcat ttgatttgaa agaaatgatg
      481 aaggctcatg ccgcaaaagg aaaaacagtc attttttcta cacatgtatt ggataccgca
      541 caacagttat gcgataaact ggctattttg aagaaaggtg aattgattta tcagggggca
      601 gttagtgaat tattgaatgg ctcacctgat gaaacgctag aaaagattta tttgaaaatg
      661 gctggaaggc aagcgacagg agatggaccg tatgtataag cgacaattat tagaattaat
      721 tcgagttaat cttttatatg ttaatccaca gctcacgaat aaagcaagag agaaaggaaa
      781 aaagggaaag cgattaacta tctatctttt gtctcaaaat cttttttcag ggctgatttt
      841 tttactgatt tacggatttg ctatgtttgc tatgaacttt gttaaattgc caggtttctt
      901 tacttattac gtggcacttt ttggtatgtt aggtttaact caaagtatat cagtcattta
      961 taatatcttt tttgaaggaa atgacctgca agtatttttg cctctgcctt ttggacaaga
     1021 acaggttttt tcagcaaaga ttttggtagt tgctttgacc atcattcctt ttgtttttcc
     1081 gcttctagtc atgtttattt taaccggatg gcgagctgat ctagggattt tgcttactat
     1141 cattatctca atccttttgt tttctctatt tttgattatt cttttttgtc tggctagttt
     1201 gattgttttt ggtttggctc gaacagcagt gtttaaaaaa catagaaaaa cagtttctag
     1261 tatcttgctt ggcagctcga tgctgatcgc atttgctgga atcatgtgga tgaatcatca
     1321 aacggggact attgaaaatg agttaacaga tcggcatccg atttcgatat tattgccatt
     1381 ctatgatgct gccagtcagc cactttctca acaaggttta ttgggttggg gaatactagc
     1441 tatcacggct ctgggatttt tccttgctgt aaaacagctg atagtaccaa agcttacaga
     1501 gcaactgact gattcaaaca gtgagttcaa aataaaaaga acccataaaa agaaccaaaa
     1561 tgtgcatcag cttttattta gttacaatgc tcaattagta aaagaaccta atttgattat
     1621 gcaagttctt tcttcttcct tgttaactcc aatcattttt attcttgctt ttgccctagg
     1681 aggcgaaata aaattaactg atttagataa taagttaatc ggtgttgttt tcctggtagg
     1741 gatcgcattg gctttcttaa cagttaatca gacttctttt atcagcaatc ttatttcttt
     1801 ggatcaagaa aattttctgt ttatccgttc tttgccaatc tctatgaacc aatatttaaa
     1861 agaaaagttc agattcggat ggatattgca gagtttttta actggtggga tcgcattatt
     1921 aagcggactg tcttttcagc tgcctttgtt ttttatccct agcttgctat ttggttcttt
     1981 attcggctgc tacttattgt cactacgtta tttcgctaga gattatcggt tgctgctgct
     2041 gaactggacc aatattaacc aattatttaa cagaggttca ggaagtttgg gtttagtcgc
     2101 aactatgatg ggttcattga tcatcagtat tattcttatc gtcttatacg gaatgttggc
     2161 tttgttttat ccattttggt tagtgaatat tcctgtgatc ggtttagttc taatactttc
     2221 cgctctatgg atcgtttatt atcaacgaac gttttggaaa aaatttgaat aaagaagagg
     2281 ggccgtgaca aaaatcatgt cgcagcccct tttttacaga tagagggtgt tcaaaagtca
     2341 gtttctcagt attaagtcaa ttttatgtaa aaatgtgaga agctattttt acaaaacttt
     2401 ttttaatact cataattact tcttattgct cctgcggtta cttgtcgcaa tgtttgtgag
     2461 atattttgtc atcgctcaag gctgaacgtc tctttcacaa ccttttcttt gttacacttc
     2521 gtttatttaa aaagccgttt ttttcgtttc atcatgattg cgtcacgctt tactttgaaa
     2581 ttacggatgt tatggttaaa gacttctgat aatacaagac ctaaagcgat tgctccagca
     2641 atcataattg cttggaccgc ataatgagcc gcttctcgat aggattcagt aacgagattt
     2701 cgaaccgctt gataagctaa accaccaggg actagaggaa ccatcccggg aatattgaaa
     2761 atcgttacgg gcattttcaa acgtttagag aaaagatcgc tgatgaatgc tactcctaaa
     2821 gaaccaagca atgaaccaaa agcagcagat ccgcctattt gaacacagat ccagtagatc
     2881 atccagccaa aagcaccaga aagaccacaa caaatcaatg aacgcctagg tacatttgta
     2941 ataacggcaa atgccgcagt cgctaaaaaa ctaaaagaaa actgaataaa tatcgtcccc
     3001 atacttgtgt tcctttccta ataaaaaagc tggaaaataa aagcaatcgc aaaaccgatc
     3061 atcgtagcag tcatcatcgc ttctgttccg cgagaaactc ctgagacata atgtccagct
     3121 agcaaatcac ggatagcgtt cgtaatttga actccaggaa ctaaaggcat cacacatccg
     3181 ataatgatta gatcctgatt gcttcctaag ccaaaacgta cgctgtagat agcggctatg
     3241 ccaatcaata aagaagataa aaattccgat aaaaatttga ttctaaaaaa tttcaaacta
     3301 tatagataag ctgcgtatcc caatcctcca atgactaaag tcacaggtaa atcggtcatc
     3361 tttccgccaa ataacaccat aattgttcca ctgatcactg ccgcgctaac gatttgcaac
     3421 cagacaggaa agaaagaacg gtctcggtcg attgcttgca gttcgcagta aagttcttct
     3481 aatgttagct ctgaagcaac atatttacgt gaaagatcat ttacttttac tactttttct
     3541 aaattaatcg aacggttggt aatttgttcc attcggatgg tcgaggtacg atcaagacca
     3601 ataaagagac cggtttgagt gacatagctg accaaacgga aatttccaga agctaaagca
     3661 attcgactca tcgtatcttc tacccgatac atctccgcat tgctttccat catgattttc
     3721 ccagcgagta aacaagtgtc cagcaacaga tctaattctt ttcccgtcat agcatcttct
     3781 ccaataattg attgattagt tttattttac tacaaaacat accagaagcg taaagaataa
     3841 aaaaacttga ggaaggaata aaagaggcgt atactgtttt tgtattgcgg gtgtagttta
     3901 gtggtaaaat cccagcttcc caagctgatg tcgcgggttc gattcccgtc acccgcttaa
     3961 aataggttgt gaaaaagctg ctctgcaaca agtaataaga acagccaagc aaaaacaact
     4021 tcttatgttt ttaacttgga tggacttatt accgcagatg caagcttttg cgcaccgttt
     4081 accaagcggt tgtgaaatca gtgtattgtc ttgagtatga agaaaatttt tgaataataa
     4141 taaatacctt acagatagag tggtgactga ttagagacga ggaatccgtc tcttttttgt
     4201 atttttaagc catcttaaaa caggttttat tttttgctgt ttctatttct ttctagtatt
     4261 tatttatagg ggaacatatg ttaagattta gggagctgtt agtaaaggag taaagaaatg
     4321 agcaaaaaaa caaccaaggc gatcgtgggg atcgtcgctg ctgtgattgc aggggcattc
     4381 ggtgtttcca ccacacaaaa agattcagat atctttcagc agattacaac tatttttgat
     4441 tccacaccac aagcttctca agtagcggat gtgccagaat atgatggaaa acatcaagaa
     4501 atagaaatca atgggaatca gccggaattt acaaaagagg atcttagcct ttccaaagga
     4561 acatgggaaa gctattcaaa tattgataaa ttgaacagag taggacaagc caatgctatg
     4621 cttggaaaag atttgttccc tactgaaaag agagagcctt tgtatattga cccgacaggt
     4681 tggaaacaaa agaaattaag tgacgggcaa tggttatata atcgcagcca tttaattggt
     4741 ttccagctga ctggtcaaaa taacaacatt aaaaacttga tgacagggac acgttcgttg
     4801 aatgctccat acatgcttgc tcacgaaaat gatattgcgg catatatcaa agaaacaaat
     4861 catcatgtac gctaccgtgt cactcctcat tttgagggga atgaactggt agccagggga
     4921 gtgcaattgg aagcagaaag cattgaagac aaaaaaattg aattcaatgt ctttatctac
     4981 aatgttcaag atggctacac gatcaattat gagacaggac aagcaacaaa aacaaaaaat
     5041 tagtagcagc tattcgaatt ttttatgaat attatgaaaa aattgtgtaa aaacatacat
     5101 tcaaagaaat atagcgtata ctttatgaaa agagtacttt tgataaagct acagaaaaaa
     5161 ggaaaaaaac atacaaatag tgtttcttaa tttattcgga agtttggaat atttgtttgt
     5221 agttttccct ttcttcgagt aaaataaggt tgtagaaggt aagctggaat taccttgcgg
     5281 gaaagaggtg tagaatcatg ggttttacag atgaaaccgt acgttttggt tttgacgata
     5341 gtcgcaaaaa agaagtgagc gaaacattag cgattgttta tcgggctttg gaagaaaaag
     5401 gctacaatcc aatcaatcag attgtcggct acttgctttc tggagacccg gcctacattc
     5461 ctcgttatcg tgatgctcga aatttgattc gccgtcacga acgcgatgag atcatggaag
     5521 taattgtcaa ggactacctc tcaaatcatg gagtgaattt atgagagtaa tgggcttgga
     5581 tgtcggttct aagacagtag gtgttgctat cagtgacccc tttggctgga cagctcaagg
     5641 agtagagatc attcggatca atgaagcaga agaagagttt ggctttgatc ggttgaagga
     5701 attagtagca caatatgaag tagaccggtt tgtagttgga ttacctaaaa atatgaacaa
     5761 ttccatcgga ccaagggcag aagcttccat gtcatacgga gaaaagatca aagaactatt
     5821 ctccttgcca gttgattatc aagatgaacg attaaccact gttcaggcag aacgtatgct
     5881 agtagagcag gcaaatactt caagagccaa acgaaaaaaa gtcatcgata aattagcagc
     5941 tgtgatgatc ttacaaaatt atttagacgg ctcaggtaag caattatttt aataataaaa
     6001 gaaaagaggt aatcttatga cagaacataa ccattcccac gaccatgaag gacacgaaca
     6061 catcacattg gtggatgacc aaggaaatga aacattatat gaaatcctac taacagttga
     6121 cggacaagaa gaatttggac gtaactatgt tcttttatat cctgctggtg tttcagaaga
     6181 tgaagatgta gaattacaag cttatgcata catcgaaaac gaagatggaa cagaaggcga
     6241 gttagaacaa atcgaaacag acgcagaatg ggacatgatt gaagaagtat tcaatacatt
     6301 catggcggaa gaagaagaat agaacaaaag aatgaattaa taagatataa aaaaagggat
     6361 tgtgacaaac gtcttgtcac aatccctttt tccaaataaa cgatgttcaa tcagctgccc
     6421 ctcggcattc gctcgaaaaa cggcgaaata tgaagaacta ttttgccatt tgtttctcta
     6481 ataatcaggt caaaacgttg acttcacagt ctcttcc
//
LOCUS       DACEXQ010000154         6498 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.154, whole genome shotgun sequence.
ACCESSION   DACEXQ010000154 DACEXQ010000000
VERSION     DACEXQ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6498
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.154"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            89..1024
                     /locus_tag="IXH04_002077"
     CDS             89..1024
                     /locus_tag="IXH04_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1852652.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            complement(1109..2119)
                     /gene="gap"
                     /locus_tag="IXH04_002078"
     CDS             complement(1109..2119)
                     /gene="gap"
                     /locus_tag="IXH04_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ1852653.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            2407..3720
                     /gene="obgE"
                     /locus_tag="IXH04_002079"
     CDS             2407..3720
                     /gene="obgE"
                     /locus_tag="IXH04_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAQ1852654.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            3941..4528
                     /locus_tag="IXH04_002080"
     CDS             3941..4528
                     /locus_tag="IXH04_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAQ1852655.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            4705..5646
                     /locus_tag="IXH04_002081"
     CDS             4705..5646
                     /locus_tag="IXH04_002081"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAQ1852656.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            5643..6431
                     /locus_tag="IXH04_002082"
     CDS             5643..6431
                     /locus_tag="IXH04_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1852657.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            6432..>6498
                     /locus_tag="IXH04_002083"
     CDS             6432..>6498
                     /locus_tag="IXH04_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAQ1852658.1"
                     /translation="MKELKKQSNVILGFVLVLIIVL"
BASE COUNT         2099 a         1116 c         1486 g         1797 t
ORIGIN      
        1 gttataaaaa tgaacattgc atagtagaga ttctttgatt atactaattc aaggttatat
       61 gaaaaaagag aaatgagatg gatgatttat gaaactttgg atgaaaatcc ttttaggcgt
      121 cgctttagct ctgattgttg cgttagtgtt tgcagggaat tatttttata attatgctgt
      181 tgtgccttct gagaaagact ttttagacga agatacacct ggtacaacac agacatccaa
      241 agaaacggat gcacataaat ggtttagttc aaaagaaaac cgaagcgatt ggaccatcac
      301 ttcaaaagac ggtctgaaat tatcagcgat ttatcttcct gctgaaacga aaagtgaaaa
      361 aacagctctt gtggctcatg gctatatggg aaacgctgaa acgatgacca attacgcaaa
      421 gatgtttcat gatatgggtt acaatgtcct tgttcctgat gcaagaggac atggaaaaag
      481 cgaaggggat tacattggat ttggatggcc agagcgtaaa gactatgtac agtggataaa
      541 caaagtgcta gaaaacaatg gaaaatcgca agaaatcgtt ctatacggtg tcagcatggg
      601 agcagcaaca gttatgatga ctagcggaga aaaactacca gataatgtga aagcaatcat
      661 tgaagattgc ggttattctt ccgttcatga tgagcttgct tatcagctag acgatatgtt
      721 tagtcttcca gcatttccgc ttatgcaagt aacgagtcta gtgacaaaag tccgtgctgg
      781 ctactttttc ggtgaagcaa atgcagtgga acaattgaag aaaaaccagc gcccaatgct
      841 ctttatacat ggagacgaag atacatttgt accatttgaa atgttagata aggtctatcg
      901 tgcaacgaag ggaccaaaag aaaagtatgt tgtaaaagga gccgagcatg cggaagcata
      961 caagacagat cctgaaaaat atcagcaagt agtccaacaa tttttaagcc aatatgttca
     1021 ttgaacgagt ttaaaagtag gggatgaaaa gaaagaagcc tttgactgga tccagtcaaa
     1081 ggcttctttt atcgattgat ctatcaaatt acaagttagc gaatttttct agtaagcgaa
     1141 tcatttggca agtaaatcca tactcattgt cataccatgc aacagtttta acaagttgga
     1201 aatcaccagc agttgttact tctgtttgcg ttgggtcaaa gatcgaacct tgagttgttc
     1261 caatgacatc gctagaaacg atttgacggt catcataccc gaatgaagga ttgtcaactg
     1321 tgtgtttttt gattgcttcg tttacttgat cagcagttac ttttgttttc aatactgaaa
     1381 ccaattcagt taatgaacca tcaacgactg gaacacgttg tgcgtgtcct tgtaattttc
     1441 cttgtagctc agggataact aagccgattg ctttcgcagc accagtagaa tgtgggatag
     1501 tattgtcagc agctgaacga gcagcacgca aattaccgcc ttttacaggt ccatctaaga
     1561 gcatttgtgt ggaagtgtag gcatgaactg ttgtcattgt accaacttcg atcccaaatt
     1621 cattatttaa gaaataagcc ataggtgcta aacagtttgt tgtacatgaa cctgctgaga
     1681 tgattttgtc gttttcatct aatgtatcat cattgacgtt atagacgatg gttttcatag
     1741 caccagctgg ggcagaaata actactcgtt tgacaccagc atctaaatgc gcttttgctt
     1801 tttcttcaga tgtataaaaa ccagtacatt ctagtacgat atctacaccg ttttcttttg
     1861 cccaagggat tttgcttgcg tcgggttctg cataaacacg agttgcttcg ccatccacta
     1921 cgattgagtc ttctgttgca gtaactgtgc caggataagt accatgagta gagtcaaatt
     1981 gcaataattg tgctaacata gttggacttg ttaagtcgtt gattgctact acttcaatat
     2041 catctgagac ttctttgatg cggcggaaag ccagacggcc aatacgccca aatccgttaa
     2101 tacctacttt tactgtcata tcaaaagttc ctcctttatt aggggtgctg tgaaacggca
     2161 tcctcaatta atacaattaa tacactgtta ttctgctacg caaagaatat tttgtcaaaa
     2221 aattatactt ggtagcagaa aaaaggttgg aaaccttacc aaatgataaa aaaagaagaa
     2281 ggtaaacaat ctttttgctg aaatgataga caaaatcaga tttcaccgat aaaataagta
     2341 agacttttgt ttattgtgga aattcaggca gtcaaataaa taaatgaata ataggaggac
     2401 ttaattatgt ccatgttttt agatcaagta acgatcgatg tgaaagctgg caagggcgga
     2461 gacggaatgg ttgcatttcg tcgcgaaaaa tacgttcctg acggcggacc ggctggcgga
     2521 gacggtggtc gtggcggcga tgtcatcttg atcgtcgatg aagggttacg tacattgatg
     2581 gattttcggt tcaatcgcca tttcaaagcg cagccaggtg aaaatgggat gagtaaaggg
     2641 atgcacggtc gtggttcaga agatacctat gttaaagtac cacaaggaac cactgtccga
     2701 gatgcggaga ctggtgctct tttaggagat ttaattgaaa atggtcaaac actagttgtg
     2761 gcaaaaggtg gacgcggtgg acgagggaat atccgttttg cctctcctcg gaatcctgca
     2821 ccggaaattg ctgaaaacgg tgaaccagga caagagcgaa aaatcgaact agaattgaaa
     2881 gtattggcag acgttggact tgtaggtttt ccatctgtcg ggaaatcgac tttactatcc
     2941 gttatttcat ctgctcgtcc aaaaatcggc gcttatcatt tcacgacgct cgtacctaat
     3001 ctaggaatgg ttactacaag tgatggcaga agttttgccg cagcagactt gccaggcttg
     3061 attgaaggag cttcgcaagg tgttgggctg ggtacacaat tccttcgtca tatcgaaaga
     3121 acacgagtaa ttttacatgt gatcgatatg agtgggatgg aaggacgaga tccttatgaa
     3181 gactatcttg ccatcaacaa agaattatct acttataact tacgcttgct agagcgtccg
     3241 caaatcatcg tggcaaacaa aatggatatg ccggatgcac cggaaaatct agtgaaattc
     3301 aaagaacagc tgaacaagga aaaagaagat gaatttgctg atgacatccc agtatttcca
     3361 atttctggag tgactcgaca aggattggac gcgttattga atgctacagc agatttgtta
     3421 gaagtaacgc cagaattccc attgtacgaa gaagaactgg aagaagaaac tgtacactat
     3481 ggattcaatc cagaaggtcc tgagttccaa atcgatcgtg attcagatgc aacgtggatc
     3541 ttatctggtg agaagatcga aaaattattc caaatgacga atttcgatca tgatgaaaca
     3601 gtgatgcgtt ttgctcgtca attacgtggt atgggtgtgg atgaagctct tcgggcacgc
     3661 ggtgcgaaag atggcgacct tgtacgtatc ggtgagtttg aatttgaatt tgtcgaatag
     3721 gcaagcaaat aaaaacaaat accaaaaaat atagattcag atttaaggga ggttgtgaca
     3781 gaagtgatca gcttcaagaa cgccgtttat tcggaaaaag gagctgtgat acgacttttg
     3841 tcacagccct attttattgg aaaaaaagag tgcttttctt ttcttatttt tcaattaaag
     3901 gtaaaataaa taacaagaag gtatgaaagg ggcgttttta atgagcaaac gagtaatcat
     3961 tttaaatttt caagtagaaa gccaggcata tcaggcattt tcagaaatca aaaagttaca
     4021 tgctgttcgt tcgattaaag gagaacagat ggcagttgtc agccatgatc ctaatgggac
     4081 acatcaattc aaaatagaag attttatcga ctttaccggt aataacaaat cttctacggg
     4141 tggattaatc ggaatgttgg ttggtgtatt aggtggtccg ctagggattt tattaggatg
     4201 gtttgccgga ggaatgatcg gtgcgacaca agatgccaaa gaaataaaag atgcccagac
     4261 cgtgttcgac tttgtaggaa ataaaatcgg tgaaggagct acaggactga tcttgattgc
     4321 tgatgaagag gacaatcgcc cactgaatca attggtaatg atggaattag gcggagaaat
     4381 tactcgcttg gattatgaag atgtcgaaaa agatgtgcat gatgcgaaag aagctgaaaa
     4441 tcatgcgaaa gaagctgcac aaaagacttg gaaggacaaa catccgcaaa cttccaacga
     4501 caatgaaagt tctggatctt caaattaata aaaaacagca gaggatctca aacgggttcg
     4561 ctgctgtttt tatagtaatt ttcaaagaaa acgctatcgc tttagaacgt tatttttaat
     4621 ttgaacccat tctcgactta cggttgatta tcccttcttt ttaccctaaa attaaagagg
     4681 aacttaaaga aagtcaggta gattatggaa ttacaatttt taggaacagg tgcaggtgtt
     4741 cctgcaaaac atagaaatgt gacaagtata gcattgaaac ttttagacga gcgcaatgaa
     4801 gtttggttgt tcgactgtgg cgaaggcacg caaatgcaga ttttgcgaac gactattcgt
     4861 ccgagaaaaa tcggtaaaat tttcattaca catttacacg gcgatcatat ttttggactt
     4921 cccgggctca tcagcagccg gtcatttcaa ggtggggata cgccattaga gatttacggg
     4981 ccaaaaggga tagaggaata cataaaagtc tctttaggta tttcgcaaac gcgtttaagt
     5041 tatccgctga aattcattga gctaaatgaa acagccccga tttttactga tcagcaattt
     5101 tctgtttata caaaaaaact aaatcatggg atcgacagtt ttggttatcg tgtggttgaa
     5161 catgatcata aaggagaatt gcaggtggat cgtctaaaag aattaggtat tccagctgga
     5221 ccactgtacg gcaaactgaa gcaaggtgaa acgatccaac tagaagatgg gcggacgatc
     5281 aatggaaaag atttcgtagg tccagataaa aaaggacgaa tcgtgactat tttaggcgat
     5341 acgagaaaaa cacataattc cgttgtgctt gcggaaaata gcgatattct ggtacatgaa
     5401 agcacattta ataaagatga agcaagaatg gcgcacaact atttccattc cacgactcat
     5461 caagctgctg aagttgcaaa agaagcgcag gcaaaacggc tgctacttac tcatatcagt
     5521 gcgagatatt taggaaaagc agctttagag cttgaaaaag aagcacaaga agtgtttgaa
     5581 aacacgacga ttatgaaaga ttttgatagc attgagattc catttcgcga ggaggaagag
     5641 gcatgaattt agaagataaa gttgttttag taacaggtag ttcaggcggt ctgggcgcac
     5701 aaatctgtta tgaagcagca aagcaaggag caatcgttat aagttgcgca cgaagaatgg
     5761 catttgtaga aggtgtcaga gacgagtgca gacgcttgag cggcaaagag gcttatgctt
     5821 tcaaggtaga cgtcagcaat cctgaaagcg tggacgaatt atatgaaaag gtgatggaag
     5881 aagtcgggcg cgtcgatatt ctggtaaaca atgcgggttt tgggattttc gaagacttcc
     5941 ttacattcga ccttggaaaa gcatacgata tgtttgaagt caacatctta ggaatgatgg
     6001 ttttgacaca aaaatttgcg atcgatatgg cagaaagaag acaaggtcat atcattaata
     6061 tcgcctctat ggctggaaaa atggcaactg caaaatcaac ggtttattct gccacgaaat
     6121 ttgcagtcct tggcttctcc aacgctttgc gattagaatt gaaaccactt ggcgtagctg
     6181 ttacgacagt gaatccaggt ccaatcgaga caaatttctt cgataaggct gatccaagcg
     6241 gaagctattt ggaaaaagtc ggacaaatcg ttttagaacc tgccaagttg gcaaaaatga
     6301 tcgtccgcaa tatgagacat ccaaaacggg aaatcaatca accgtttatt ttagaagttg
     6361 catcaaaatt ttatacgctg tttccaacga ttggtgatta tttagccagc ggaattttta
     6421 acaaaaagta ggtgaaggaa ttgaaaaagc aaagcaatgt gattcttgga tttgttttag
     6481 ttttgattat tgttcttt
//
LOCUS       DACEXQ010000155         6441 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.155, whole genome shotgun sequence.
ACCESSION   DACEXQ010000155 DACEXQ010000000
VERSION     DACEXQ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.155"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(189..851)
                     /locus_tag="IXH04_002084"
     CDS             complement(189..851)
                     /locus_tag="IXH04_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1852659.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            1035..2681
                     /locus_tag="IXH04_002085"
     CDS             1035..2681
                     /locus_tag="IXH04_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAQ1852660.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            2672..3754
                     /gene="uxuA"
                     /locus_tag="IXH04_002086"
     CDS             2672..3754
                     /gene="uxuA"
                     /locus_tag="IXH04_002086"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ1852661.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(3862..5415)
                     /locus_tag="IXH04_002087"
                     /pseudo
     CDS             complement(3862..5415)
                     /locus_tag="IXH04_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
     gene            5563..>6441
                     /locus_tag="IXH04_002088"
     CDS             5563..>6441
                     /locus_tag="IXH04_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ1852662.1"
                     /translation="MRYSTILISTFAALSLSLTINQTVHTEETETAKWTETKWANVNR
                     ITFQDEFSSFNSGVAKLRKGQTATIKAKIDYSGDLAPILQSAVTFENVDPTLSIGYDE
                     NTLTIQHKQAIFTFTVTLNQDLLKPALFTVKVSDGLPNDHHLTPYSQRQTIEQDSAID
                     SGGDLVEEPTDKPENENKPEVPPTENPDGEQKPEIEPGEEPDTETQPEPDNKSKPEIT
                     PGEKPDVTPEPDTDSGNQTVPETNPDTDNETENPEKPEVDPEEKPDVTPEPDTDARDQ
                     GIPEKINKKTIQEDGKK"
BASE COUNT         2173 a         1125 c         1206 g         1937 t
ORIGIN      
        1 agcatgtgca ttaaattctt cgattttttt cccaatcaat gacatagtaa aacctccaat
       61 aataattatt tatttataat tattgtaatt tagttggagt tgcaaagtca atggatattt
      121 tgttatttaa taaagattac tcaaaaacga aggttaatta taaaaataga agaggtcgca
      181 agaaaatgct actgctggaa atactcggga taacgttgag taacagtttc ctgttctttc
      241 agcatcatgt gtaagtgcaa tcccgtttga aatcgagctt cttctggttt tttatcaact
      301 aaagccttga gtaaatcata atgttgttcc atgatcgttt cccatggaag cccctgaatg
      361 gtcactcgta accagcggaa acgttctaaa tgggtattat ggtcatctaa ccagttccaa
      421 acttcttttc ttcctgcaat ttcgaaacaa gcttcatgaa ataaattatc tgcacggaaa
      481 aaagcgcgtt cgtcatttat tttgactgct ttttcttgtt cttctaaaat cgtttctagc
      541 actattcgtg tttgtgtgtc caatttggaa caacattcaa tcatgatttg ctcttccaac
      601 tgttctcgga caaagcgagc attttctgca gaaacaagat taattttgga aacatacgta
      661 ccactttgag gtatcgtata cacgagttct tgctgacgta gttgaatcaa tgcttcgcga
      721 atgggagtcc ggccgatagc aagatgttcc tccagtgcct tttcagatat tttccttcca
      781 ggttctaagt cagcatagat aatttgttta cgaatcgctt gataagcttg tgattgcaag
      841 ctgcttttca tctttattcc tccaaaatca tttctatgaa cgtagtataa cagaagaggt
      901 ctgaaaaaca atctggtatg tcacattaac tagtaaattt gtataaatag taaaattttg
      961 gttgacatag cgatttttat tgcataaaat tcacttgaat acaaaaacta atatgttagt
     1021 ttaggagggg aaaaatgatt gatttaaaca actggaggaa ctttatccct gaattatctc
     1081 aaaaagaagt agaaatacct cattttgacc gtgcatcatt aagagaaaat ggacaaaaaa
     1141 atcctgtctg gatccatttc ggtggcggaa atctttatcg aggatttcat gcagaaatcg
     1201 ctcaacgttt agcggatcta ggcgaattgt ccactggtgt cattgtttgt gaaacattcg
     1261 atgaagaaat aattgaaagg gcttaccaag attttgatga tttgattctt gaagtggtca
     1321 tgccaagcag tggagagttc gtcaaacgta tattagctgc gacagctgac agtctgtatt
     1381 gtcatccaga ttgttctgaa cactttgata agatgattcg acactttgaa aatcctagtt
     1441 tacaattagt tacttttgct attactgaaa aaggttatcg attgaaagat acaaataata
     1501 aatatatagg accgattgca gtcgatataa aaaatggtcc aaagaagcca tcacatacaa
     1561 tgagtattct tgctgtgcta ctttataaac gttttttatc tggagcttgg ccgattgcac
     1621 ttgtctctac agataatttt tcacaaaacg gtagtcaatt acgtacttct ttgctaacga
     1681 tcgcaaaaga atggcaaaag gaaggttatg tacaaaatac atttatccaa tacttatcga
     1741 atgaagaaat tgttgcgttt ccatggacga tgattgatcg tatcaccccg aatccttcct
     1801 tgtcagttag caaaatgttg gcaaaagagg gcttttcaaa tatgtcgatt ttccaaaccg
     1861 caaaaggaaa taatattgct ccatttgtca atacagagga catccattat ttaatcatgg
     1921 aagatcattt tcccaatgga cgtccgaaat tcgaaaaagc tggggtattc ttgactgatc
     1981 gtgcaacggt agataaagca gatacgatga aggtgacgac ttgccttaat cctttgcata
     2041 cgtctttagc tattatgggt tgtttactgg ggtataggag catagctgag gagatgaaag
     2101 atccagattt atcgctgcta gtttctgaaa tcgggtacaa tgaaggcctc ccagttgtgg
     2161 agtctccagg tatcattgat ccaaagatct ttctggaaga agtaattact gtgcgctttt
     2221 ctaatccaaa tattcctaat atgcctcagc gaatcgccac ggatacctct caaaaggtgg
     2281 cgattcgtta tggtgagacg attaaaaaat atatggaaat agaagttaaa gacccgcaga
     2341 cacttgtatt cataccattg actctcgctg cttggcttcg ataccttatt ggcattgatg
     2401 atcaaggaaa accattcatc ccgagtccag acccactatt gacagacctt cagcataagc
     2461 tttccgatat tcaactgggt acgcaagata catcacatat tcatgagaaa atggctccta
     2521 ttttgtcaaa taaagaaatt tttggtgtag atctttacca agcagagctt ggcagaaaaa
     2581 tagaagaaat gttcatcaag atggtagcag gaccaaatgc cgtacgaaaa acattacagg
     2641 attacttaca agaacatgga ggaaatttat catggaaatg acttttagat ggtacggatc
     2701 acaggaagaa aagattacct tagatgaaat cagacagatt cctgcggtca aagggattgt
     2761 aggcacattg atggatatac cagtaggaga ggtatggcct agggagagga tcaaagaact
     2821 aaaagaagaa attgagtctc gtgggttaac actaaaagta attgaaagcg tcaatgtgca
     2881 tgatgccatt aaaattggat taccttcccg tgagcagtat atcgaaaact ataagcagac
     2941 aatccgaaac ttggcagaat atggaataga agtcatctgc tataatttta tgcctgtatt
     3001 tgactgggtg aaatctgacc ttgactaccg cttagaagac ggctcatcca cgttagcttt
     3061 tatatctgat gatattccag atgatccaag agaaatcgtc gcgcgcatcg aacattccag
     3121 caatgaattt ggattgccgg gatgggaacc tgagcgctta gctcctatca aatccttgct
     3181 tgaagcctat gcatcagtag acgaagaaaa acttcgagaa aattttgcct attttctcca
     3241 atcaatcatc ccaacatgtg aagaggtagg ggtcaaaatg gctgtacatc cagatgatcc
     3301 gccatactcg atttttggat tagctcgaat cgtaaagaat cgagaagatt tagattggtt
     3361 gtgtcgcata gtagacagcc cttataacgg aatcactctt tgtacaggga gtattgcaga
     3421 agatccagaa aatgatgtat atgggatttt agcagaattt tgccaaagag atcgaatccc
     3481 atttgcacat gttcgaaata ttaaattttt agctaatcga gatttctatg aggcaccaca
     3541 taaatccgaa tacggttcat tagacatgta ccgcatacta aaaacaatgt atgacaacgg
     3601 atttaacgga tacatccgtc ctgatcatgg aagaatgatc tggaatgaga caggaagacc
     3661 aggatacggc ttatatgatc gagcattagg tgctgtttat ctaaatggat tatgggaagc
     3721 aatcgaaaaa ggaaacaaaa gtaaagataa ttagtgaaaa aaactgtaat tttattatgt
     3781 aaataagaag gatgttcaaa ttcttgatac ataaatccgg aggttgttaa taagcattcg
     3841 gatttatgta tcttatttgg tctaggaagt aacttgattt tcttttttcg attgatccat
     3901 gagtgcttct tcttttaatt caaagatata ttctgagatc ctcataatca cttttttcca
     3961 gtcttctggt gtgatgattc cttttaaaat atagattttt ttgatatgag aaggcaattg
     4021 tttcaaaggg cagtcgctga taacgatgtt gcagttctcc ataaaatcat tttgtatttt
     4081 gatcgattca tttgaaaaaa tttgttctat cttaacgatc agatctttac tgatataaaa
     4141 atcacgcgta tagttcacac caatcacaat tcgcggctgt tttttgtagc gatcatacaa
     4201 atgtactagg tcttcaacca tccattgcac attttccttt tctaaaataa ctggtaacaa
     4261 gtgttgattt tgttctctga aatccatata aaattgttca atggcggttt ggacttcttt
     4321 caatacgcta tgttcttgat aaagcatgaa agtaggaagg ggcgtatttg gttgattgaa
     4381 gttaagaaat ccattataaa gtttatttcg tgcgaatgcc aatgagtaaa gaatcttttc
     4441 tttttcaggt ataaacaaat caaatacagt acaaagggac tccaccattt ctcttgaata
     4501 aagaacatga ggaacgtttg ctttttttaa aagtttgtat tgctccaagc ttgtttcttc
     4561 cgattcgatt ttcgtgatag aaagtcgagc taaaatattc actaagatgc gttgttcgcg
     4621 acaaattggc agattaggtc gcaaaatatc tacagagaga tcaataaaaa agtcaatata
     4681 ttcatactct gtgaattctt tttgtacgtc tttcacagaa gttgcaggaa aattctcttt
     4741 acatattttt aatagaaaat ataatttatc tatcataccg ctatttagtc ggtcaagtac
     4801 ttctttttta aaaaaagtat tcatcataaa agccactact tgcggatctt catcataaaa
     4861 ttctgtagca gtacttgaaa agatgttcca ataaatatct aatgctgcat attgaatttg
     4921 tacctctttt ccagaaaaag actttctgcc attattagaa aaggagatag acaaaccgta
     4981 ctatgctaaa aatgcattaa tctttttcat tttcgtatac aaataagagg cactatagtt
     5041 tgtttgctga ctgaaactga taatcgaatt tgtctgacag ctgaacaaag aagtcaagat
     5101 ctgataactg actgattcgt tgcagtatct tccaaataga cgataatagc agtaagccgg
     5161 atcaattcct tcaaaattca actttaaaaa aggtttttgc ttatcaatat gcagttctgt
     5221 tagattgaga cgctcaaagt cttgttctaa ttctttgatg tatagtaaaa aatttgattc
     5281 agacatatgg aaatgatctg ctaaatattg tgtagtaata aagaaacgat gttcagatag
     5341 gaaaagaaaa atatctaatt ttgtattgct ttttttcttc aagataaacg ttttaatcgc
     5401 ttcttgagtt tgcatgaaag aggctccttt ttacttaaat tgttaaaact tgaaaatatt
     5461 actgtttttt tcataaattt agacaaaaaa acagtaaaca tataggtaga atctctacta
     5521 tcttatttct tgcatacaaa aaaatcaagg agtgagagag ttatgagata ttcaaccatc
     5581 ttaatatcaa catttgcagc cttaagctta tcgcttacta taaatcagac agtccacaca
     5641 gaggagacag aaacagccaa atggacagaa accaagtggg ccaacgtcaa tcgaattacc
     5701 tttcaggatg aattttcatc cttcaattca ggagtcgcaa aattaagaaa aggacaaaca
     5761 gcgaccatca aagcaaaaat tgactattcg ggagacttag cacctatact acaatcagct
     5821 gttacatttg agaatgttga tcccactttg tcgattggct atgacgagaa cactttgact
     5881 atccaacaca agcaagcgat atttaccttc accgttacat taaatcagga cttgttaaaa
     5941 ccagcgcttt ttactgtcaa ggtatcagat ggactaccga acgatcatca tttaacgccg
     6001 tatagtcagc ggcagacaat tgagcaagat tcagccatcg actcaggagg agatcttgtt
     6061 gaagaaccaa cagataaacc agaaaatgaa aataagccag aagtaccgcc tacagaaaat
     6121 ccagacggag aacagaaacc ggaaatagaa cctggagaag aacccgatac agaaactcaa
     6181 ccagagccag acaataaatc aaaacctgaa ataacccctg gagaaaaacc agatgtaact
     6241 ccagaaccag atacggattc tggaaaccaa acagtaccgg aaacaaatcc agacacagat
     6301 aatgaaactg aaaatccaga aaaaccagag gtagatccag aagaaaaacc agatgtgact
     6361 ccagaaccag atacggacgc aagagaccaa ggaataccag aaaaaataaa caaaaaaaca
     6421 attcaagagg atggaaaaaa a
//
LOCUS       DACEXQ010000156         6418 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.156, whole genome shotgun sequence.
ACCESSION   DACEXQ010000156 DACEXQ010000000
VERSION     DACEXQ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.156"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(88..1218)
                     /locus_tag="IXH04_002089"
     CDS             complement(88..1218)
                     /locus_tag="IXH04_002089"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAQ1852663.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1867..3069)
                     /locus_tag="IXH04_002090"
     CDS             complement(1867..3069)
                     /locus_tag="IXH04_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1852664.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3129..3332)
                     /locus_tag="IXH04_002091"
     CDS             complement(3129..3332)
                     /locus_tag="IXH04_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ1852665.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3474..4706)
                     /locus_tag="IXH04_002092"
                     /pseudo
     CDS             complement(3474..4706)
                     /locus_tag="IXH04_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4869..5681
                     /locus_tag="IXH04_002093"
     CDS             4869..5681
                     /locus_tag="IXH04_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1852666.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1919 a         1313 c         1009 g         2177 t
ORIGIN      
        1 ataaaaggta aataaaaaag agtggatttc tccactccat gaaaagccga ctttttggga
       61 cgcttttttt tgccaagcct cacattttta tagccaaact tcttttgcga cttgctgcaa
      121 taattggatt ttcttccatt gttcttcttc tgttaaatgg tttccttctt ctgttgaagc
      181 aaacccacat tgaggactga tacttagatt ttccaatggt acaaattgtt gtgcttcttt
      241 tattcgttct tttattttct ccgtatcttc caaatctgga aacttagaag taactaatcc
      301 taaaacaact ttactttctt tgttactcca atgtcttaaa ggagtaaaat ctcctgctcg
      361 gtcagaatcg tattccaaaa agaaaccatc ataatttgtt gaaaacaact cttctgcaat
      421 gggttcatat ccaccggaaa acagccaaga agaagcgtga ttcccgcggc agatatgtgt
      481 tgtgatcgtc aaatcttctg gtttgtcttc cagtgcgtta ttgataatct ctgtacaaat
      541 atgggccatt tctttcggat caattccttt ttgaagaagt gcttctcttt tagtttgatc
      601 agctagaaac gcccaatttg tatcatcgaa ttgcaggtaa cggcaacctt tttcataaaa
      661 agcatgaatc gctgctttgt aagtagagga caagtcctca taaaaatcag atagatcagg
      721 ataaatctcc ttgatttttg aagaaccatc attagatagg atctcttgtc gtaaaatcat
      781 cgtgggacta gggatcgttg cttttgcaac aaaagtatca tctgtttcca ctacttcttt
      841 cagaaaagca aaatctttta aaaatgggtg atttggattg aaagataatt tatctactac
      901 ccgaatatta taagctggtg ctgcttttcc gtggaatgtt tgattatatc cagtttctgg
      961 aacatagcct tcgataccat ttagttcttc taaaaaatca agatgccaaa atccacgccg
     1021 aaattcacca tctgtaattc ctttgatgcc gactgctttt tggtcttcca ctaatttttt
     1081 tatttcctgg tcttcgattg cacgaagttc ttcttttgaa agtaagcctt cttgaacttt
     1141 ttttcttgct tctttgatcg attgcgggcg taagaaactt ccgacatgat ctactgtaaa
     1201 tggtctattt ttgctcattt cactcactct tttctttgtt ttttgtaaaa aaaaatcctt
     1261 ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt tttcagaaaa
     1321 aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa agactgttcg
     1381 tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc aacttctagc
     1441 tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct tctcatcgga
     1501 tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg gaataagaac
     1561 aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata aagcttttct
     1621 aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa tgatcatacc
     1681 cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa actaagtcct
     1741 ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca tcttgaacag
     1801 gagcaattcg actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg tagaaccgtg
     1861 gaaaactcag actggtaata aattgtcatt ttttgaaaga atagcgccct cttgaacatt
     1921 atcaatcgca cttaccactt gttgatctga ctttgcatat aagtgaccgt aagttcctaa
     1981 agtgatttga atattcgcat ggcccaatcg ttcttgaata gatagcggtt gaatattctg
     2041 ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg gtttgacacc
     2101 cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg taaatgttcc
     2161 gtccaactga gcaacatatt caaaatttcc aatacgtttt tgcaagtcat accactgtct
     2221 gagttttaag gaaagcttct tccccaaccc aatcgttcga taagcacatt tagttttggg
     2281 agtcgaaaag taccaatcat cttttgtatt ataaatcaat gtcttctcaa tcgttatcgt
     2341 gtttttctca aaatctacat tcgaccaaga caaagcttca agttctccaa tccgaatgcc
     2401 agtataaaag agcatttcat acacaatctt ccgatggtgg gtttgaatct tatcatcctt
     2461 caattcattc atcacttttt gaaactcact gtgttcccaa aactcaactt tcttattctc
     2521 agaaggaagc gaagaaacat cttcaacggg atttttagca atcaccccct ctttaacagc
     2581 ccgatccaat acaattttta ggtgccctcg aatccgattg atataagatt ttgataacgt
     2641 ttcgccatta acttttttcc gtgaattacg tgactctgtc tggcttagtt caaataacca
     2701 atcctcaata tcgtgacttg taatatctcg aacacgaaaa tgatagaaaa actgcatacg
     2761 cttttcgaaa atgaattgcg cattctcata tgtttgactt ttgacctgtc tctcgtacca
     2821 ttttacaaac ctatccttgt aaaactcttc gaaagtcatt tgattggtag aacacagttg
     2881 attaggaatt gtcttgttaa aatcatgtgt ttccatgtac ttgtcataag caattttggc
     2941 atctttttgt gaagcataac ctcgtttagt cttctgaacc tttcttccat tttcatctat
     3001 acctagatac gctcggaaga accatttttt tgtcttttta tcttggtaaa tatttttaat
     3061 ttttggcatc gttccattcc tccctgtact tgagaaaagc tactttaata gtaccgcgct
     3121 tgtactgttt attccgcaac ttgaatgtga aaaagatgtt caattactcg ggatggcacc
     3181 acaccaactt ggcgattatt ataaaaatca ataccttcaa tccttgcaag atatgccttg
     3241 ctctctttaa tcattcgtgc agcttgacta ggtttaaagc ccatctctat caaaattgca
     3301 cgatttacca tgaaatcaca ttttgtttcc attctctcac ctcctgattc tttactcctt
     3361 ttaaaataat cctctttata atctattgtt tctcattcga tggttgtgtt atactcgtct
     3421 tgtcgaggcg gagtacgttg cacaacttgt tgtgtaagca gtactttgct tttttagtag
     3481 cttgttttct catttgatat actttgtaat tttcgttcat aatactcaac ctcctcctga
     3541 taatactccg ggtctgcctt catattttgg agaagtttga tctgtcttgg attcatttct
     3601 ccttgttcaa taatcaaagg caacaaggaa gaatgaaacg tttgatcgac ctgtgcaata
     3661 aaaactaatg atggtgaaac accatgaatt aaccaactca tagttttctc aaaactttga
     3721 ggaattgtat ctaaagaaat ggtgattgcg ccataattgc taataaaggc tgcccatttg
     3781 gcatcaactt ttgcacctag atcatttgga tcacggtcat aaaaacaaat tgctccatta
     3841 agtaactcaa agaacagttt ggttaaatca tgggtttttg aaataatctt tgccaagctt
     3901 tgcgctttct cttttcgata ccgtagttca aagcgattct ttacttttgc ctcttctaaa
     3961 ggaattcctc gtcgcttccg ttgttcatgg tccttctgat aaaagcaaaa acgacactga
     4021 ctcttctttg agcctaaata caaggtctca ccatttgatt cattgtttat gatatccgta
     4081 ccgccatgag attcactcac ttgaaaagtg gtccacacat ggcctagtat taccttatgc
     4141 ttaagttctg gaatgctgag actccccaca aaatcatcta aagtaaaatc aatccgtgta
     4201 aagttcccct gataatctaa taccttacga aaaaactgtt gccaatttgt ttttcgtgtt
     4261 tttagccatc ttgctaagtg tttacagcct tgacctgaaa actccatcac agttccttta
     4321 accgcatcgt caatggaata ccgaatcgtg attacttgat tccacacgta ttggccaata
     4381 tagccctttg gcgcataatc cagttcctca aaacgttcaa tgggcatttc aataatctct
     4441 tcaattagct ttaaatgttg attcgagaaa aagtgaatgg ttagataatc aatcaaaaca
     4501 cggttaactg gaacgacctc ttcttgcaaa ctatccaaca caagattcaa tttttcttca
     4561 cttaattgtc tcgttccttt ttctactttc gataataact atctcgaaat ccccactttt
     4621 tctgctaaat atcgttgtgt ccaccccctt tcttctcgag aaactcgaat ggcttcaccg
     4681 agatcatgtt gattaacttg gttcatcgac aaacctcctt ctgctattta cttgtcaaag
     4741 aacctattga acttatttta agtacatttt tattgtacgc ctgtatttat tttaagtcaa
     4801 tatgttttga ctttttttaa gtacacacgt atactaaatt taagaactta tcctgaggag
     4861 gagttttcat ggagatagac aagcaagcag tcggcaatcg aattcggcag attcgccaag
     4921 aattaaagtt gagtatggag aaatttggca aactaattgg tgatttaccg cgaagtacag
     4981 tcaataactg ggaacgtggg atcaaccttc caaaaaccga aacccttcac caaatcgcag
     5041 agataggtca tgtcaccaat gaatatcttt tgtatggtga tcaagagaat caatacattt
     5101 tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa gattgaaggt ctcatagtag
     5161 acgaaatcaa acacgctgac cttgttagtg aaaaagaaat ggatcgaatg attgaatttt
     5221 ttattacaaa tctcattcca ccaactgaac aagaccagtt tactttccaa tgtatcgatg
     5281 aaaaaaagca gctttatctt ggttcaacta attttggtaa ggaagcccag ctctatttgc
     5341 aacatgataa tcaaaatcat attctgcaca tgatgccctt taccttctcc aactttagtg
     5401 tggatcgcat actcgtctat ttagcaaatc aaaaatccta ttcctatttt gggaaacatt
     5461 tacccaagga actaggtgag aaagccatcc ttctatactc aattaatcaa ttgaccagcg
     5521 atgtgcgaat tgccccgttg gtctattcag aagaaaccgc ttcttatcag tacacagagg
     5581 acaatcaata tctactagaa cagcaatatc tttatctacc attcgtgatg gaagtcgaga
     5641 aagaacgcct gttaaatgca gcgtatccat catcaaaatg aggtgacaaa attgataagc
     5701 acgtgacaaa gaaaatactg ctaggacact ccccaatggg tagttgtcac agtttcctgt
     5761 tctttgttag ccccctgtta gaaccggggg ctttattcct gcagtccggc tacgccttcc
     5821 tgcaatagac aaaactccag atcgggtaat tcgcttgtgg cgaaacccga cctggagttt
     5881 gtctctgtat tttgaaccct aaaaattaat caaacagact gatattctca taatcagcgc
     5941 gaaataagcc attaagtaca gttttcctac ctatttttga taacccgctg gttagttaat
     6001 attccttttc cgagagagat aaatgattgg ttaaatctat atacatctca acctttaaaa
     6061 atgttttcga aatacttcat cagtacatgt catacaaatt accagtgcat tctcaaattc
     6121 tagttcttta attgatttca gttgaggagc agttactttt ggcggaaatt tccatttttg
     6181 tccaaacctc ttcccccgat gaaatttctt tgttgcaatt attgcaatga aaaatttttg
     6241 tttcaaaaat atacataatt acttcctcca ataaaaacta tctacctctc aaattatttt
     6301 tctctaataa gaaaaacaga aaaggtaata agatacaaca gtgatgattc ctaataaaga
     6361 aaagactaca ataatatttt tctaacttat cgccttcaaa gaaatagttt cttatttt
//
LOCUS       DACEXQ010000157         6413 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.157, whole genome shotgun sequence.
ACCESSION   DACEXQ010000157 DACEXQ010000000
VERSION     DACEXQ010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6413
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.157"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            78..1382
                     /locus_tag="IXH04_002094"
     CDS             78..1382
                     /locus_tag="IXH04_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ1852667.1"
                     /translation="MLNDINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLS
                     QNSLFLKTCLLYLEEDEPDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPR
                     FTEMERRLLLLNVSYRLGGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILLI
                     GFAISFLRQQVCAVTIDSKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLF
                     NLCNYGFHSYQLIAEDFQQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIH
                     LMRSAWDNYQLFMPEKFYLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVI
                     ELSGILRLTKEHLTIYIVTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQ
                     QYSNRVLCERTLYTPDAVQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1474..1839)
                     /locus_tag="IXH04_002095"
     CDS             complement(1474..1839)
                     /locus_tag="IXH04_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAQ1852668.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1836..2201)
                     /locus_tag="IXH04_002096"
     CDS             complement(1836..2201)
                     /locus_tag="IXH04_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAQ1852669.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2393..2641)
                     /locus_tag="IXH04_002097"
     CDS             complement(2393..2641)
                     /locus_tag="IXH04_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852670.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2640..2768
                     /locus_tag="IXH04_002098"
     CDS             2640..2768
                     /locus_tag="IXH04_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAQ1852671.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2825..4519
                     /locus_tag="IXH04_002099"
     CDS             2825..4519
                     /locus_tag="IXH04_002099"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAQ1852672.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKMTNTLTQ
                     EEEHVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4601..4792
                     /locus_tag="IXH04_002100"
     CDS             4601..4792
                     /locus_tag="IXH04_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852673.1"
                     /translation="MDLLPQMQAFEHRLPRGCEKAALHQVIRAAKLKQLLMFLTWLDL
                     LPQMQAFEHRLPRGYEKVV"
     gene            5170..6303
                     /locus_tag="IXH04_002101"
     CDS             5170..6303
                     /locus_tag="IXH04_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAQ1852674.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQNGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYE
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
BASE COUNT         2123 a         1221 c         1169 g         1900 t
ORIGIN      
        1 tatgtgggca attctatgta aataaagaag tcaatctaga aaagctctcc aatctcctgc
       61 atgtttgcaa aaccactttg ctcaatgata tcaataacat aaaaaaagaa tttgaagaac
      121 agatcgctta tacacatcgg gaaaaggacc gttacacttt atatttttca gaacatatac
      181 ctcgatgcaa gatcatgcag caattgtctc aaaattcgtt gtttttaaaa acttgtctgc
      241 tttatttgga agaagatgaa cctgattatc ttcaattaac tgaatgtgaa ttcatctctg
      301 tcagcaaagc ttattcatta aaaaaacaag tattagctta ttttaatgac tgcgggatcg
      361 aaatcgaccg ctactcccct cgattcactg aaatggaaag acgtctgctg cttcttaatg
      421 tgtcgtatcg tttaggcggt tttaattctt gggagctacc tgaatcgttt tttgaacgtg
      481 ccgatcgttt cattgaatcc gtaacggaaa atagtggcag attttatgat aaagaaaata
      541 aagaaatctt attgattggt tttgctatca gtttcctacg ccagcaagtg tgtgctgtga
      601 caatagattc aaaatttatc gaagaaatca aaaaacgtcc tatttacaat tatgtcgaat
      661 cagcatggga aaacactgat ttccaaacct attataaaaa agaagaattc gcctttattt
      721 tgacactatt caatctttgt aattatggtt tccattctta tcagttgatt gcggaagact
      781 ttcaacagct acaccaagtg tttattgaca atactcccga aataaaagaa ctagttgcta
      841 cctttgaatc tcacttcaat caagaattat tcggaaacca accattcgaa cgtgcgctga
      901 ttcatctgat gcgttcggct tgggataact atcaattatt catgccagaa aaattttatc
      961 ttctaaatga ggaacagaca aatttatata aagaagtcca gacaattttt tccagctggt
     1021 ccagccagtt accttatgat ctacggttga atcctaattg catgcgggct tttgtgatcg
     1081 aattgagcgg aattctccgt ctgacaaaag aacatctgac tatctatatc gtcacaaatt
     1141 cggatgttca ttatctgatt tatcgggaag ccttggaagc tgtaactact ttcgattttc
     1201 aagttgcacc aacaatttat tcaagtatat ctgatataaa gaagtatgcg cagcagtact
     1261 caaaccgggt attatgtgaa cgtacattat atacaccaga tgcggtccaa tatgaaaata
     1321 ttattcctat ttcgatcaat acgatcgatc gagctatcat ttcagctgtg caaaacaaat
     1381 aagggactgt gacaaaaatc atgtcacagc ctcttatttg ttgaagatgc ccatttctgt
     1441 aacaatctct tgatacacat caggctcaca acctcactta ttttggacat ctacgggtcc
     1501 gatctcttta tttaaaaaat aagaaatgat ggttcggata actaccaacg ccgctagttg
     1561 gaaaatatcc tgcaaggtag gtttgataat cgattcgatg atcccagctg aaacaagaat
     1621 ttccaaactt agaagtatat agcctcccaa catcttttta atagcattgt tctcgctgac
     1681 tcggtcgctt tgcacttttg tatggacaaa taaatatttc aaaaaatcct tgattgatag
     1741 acaaacaccc caaaccaata aacagatagc aaagatatca agtcccagta caagtttttc
     1801 aaaatatgga agaagaaact gcatcaactc tgtcgtcatc ctacctctcc tcttctttgt
     1861 ttttctttgt cttctatttc ctgattcaag aaataagaaa tgactgttcg aatgataata
     1921 ataagtgcaa gcttcaaaat atctgtccaa gtcggcttga taactgactc gatgatatca
     1981 gcaacgacta gtacttccaa gcttaacaaa atataacttg ctagcagttt tcttgtatct
     2041 gcattacgag tattacgttc tgcatagttt ttcttgacat gagaccaatc aaaaagtttg
     2101 aatagaacaa gaacgatccc gacaattaag ataccgatag aacaaatatt taaaatagca
     2161 atgatccatt ctagaaattc aattaaattt gagcgaaaca tatacgctac ctactttctt
     2221 ttaatttgtt cctattcttt tattgtaaaa gaaagttacc ttgttcgtct atttaaaagt
     2281 tgttctaatg ataacggttc gcttaaattt ttctttttca ctctcccctt tttctccctc
     2341 catccataaa tcgtggaagt atccaaaaca acgtagcaag ttaaaataag gcttagtccg
     2401 tcatcagctt ttcaaagaag cactgtctcg gtgctttttt tgtacccaaa tttctaaaaa
     2461 tagaaccata cacctcctca aatcttttct tcttacctac cctattttta tttacaggaa
     2521 aatcagacac ttcttctttt ttgtcaaaat ttcctttttc ttctcgtcct atcatttggc
     2581 attctaataa aaacgaacta tccttggata tagaaagaga aaacaaggag gcgtttttca
     2641 tgagtaaaaa attaatcaaa gtcggcatcg gtttaggttt gcttgccctt ggcgctgcat
     2701 atctaggaaa aaagacagga ctattcgaag atgacagtca tttatatgat gaattcgaat
     2761 caatctagca tcctatttag ttgaatcact acggacatac ttggaagaca ttggaggaat
     2821 cacaatgaga aaaagtaaag caataatgat tggtgccggt attgccaata tggcagcggc
     2881 tgtttatttg attcaagaag gcaaatggaa aggggatcag atcacctttt attcattgga
     2941 tgatcacggt tcaaatgatg gtgcaccgac cgacactgta gtggatgaat attggaataa
     3001 aaaccatcca ttagaaaacc aaaaaggcta tattgctcga ggcggtcgca tgcttaatta
     3061 ccgtacctat gtagatttga tggatctgtt aagtcgaata ccttctgtta ctgagccagg
     3121 gatgactgct gaagaagata cgagagattt tgatgcaaaa caccgaacct ttgacaaagc
     3181 acgtttgatg gaaggtggca aaggaatcat tgatggcgga aaactgggat tcaataataa
     3241 agaccgtatt cttctgacta aactgattgc tatgcctgat tcagaagaag aaaaactaga
     3301 caatatcact atcgaagaat atttcaaaga tgatccacac ttttttgaaa caaacttctg
     3361 gtttatgtgg gaaacaacat ttgctttcag aacacgtagt tctgctcaag aattgcgtcg
     3421 ctatatgcat caaatgattt atgaattcac tcaaatcgaa catctagtgg gcgtcaatcg
     3481 aacacgttac aatcaatacg aaagtattat gcttcctttg atcgagtact tgaaagaaca
     3541 aggatgcaaa atcattttga accgccgtgt cactgactgg gaattcaaag aaacaccgat
     3601 gcaagatgaa atcactgtga ctggtttgaa aatgaccaat acactgactc aagaagaaga
     3661 acacgttgtg gtcgatgaag atacagcagt tattttcact aatgggtcca ttacagattc
     3721 agctacttta ggagattacg aaacaccagc tgtagaaaat atggattatg gtgcagcttc
     3781 tagtctctgg aaaaaagcca gtgaacgttt ctacaacttg ggaaatcctg acaaattctt
     3841 tgctgatcga gatgcaagcg aatgggtcag ctttaccctg acgacgaaaa accatttgtt
     3901 gttgaatgaa atcacccgta tcacgactca agaaccagga aatgccttga attcatttat
     3961 ttcaaccaca ccgatcacgc cgttaggcca aaaagatgtc aatatgtcta tcgtcgtgca
     4021 tcatcaaccg cattttacgt ctcagaaacc aaatgaaaca gttatttggg gttatttcct
     4081 ttatccaaga cgacgtggcg aatttgtgga taaacagtat attaaaatga atggaaaaga
     4141 aatgctagaa gaactgattg gacaactttc aaaagtagat cctggaccag tgaatattcg
     4201 tgaaaaagaa acagaaattt tcgacagcgt aataaataat atccctgtct atatgcctta
     4261 tgcttccgcg ttgttcaata atcgtgccaa aagcgatcga ccaaaagtca ttcctaaaca
     4321 ttcgaccaat ctggccttta caggagaatt tgtcgagcag ccttatcaga tgatctttac
     4381 tgaacaaagt gctgtacgtt ctggagaaat cgctgctttc cattttgcgg gtatccctat
     4441 gtcacgtctt gtcaaaacac cgcgttacga caaagatatt cctactctag ctcgtgccgc
     4501 aaaaaaaatg tttgaataat caagaggttg tgaaaaagct gccctgcatc aagtaataag
     4561 aacaaccagg ctaaaacagc ttcttatgtt tttgatttgg ttggacttat taccgcagat
     4621 gcaagctttt gaacaccgtt taccaagagg ttgtgaaaaa gctgccctgc atcaagtaat
     4681 aagagcagcc aagctaaaac agcttcttat gtttttgact tggttggact tattaccgca
     4741 gatgcaagct tttgaacacc gtttaccaag aggttatgaa aaagtcgttt agtttcgagt
     4801 atgaagaaaa attttgtgaa aatggttcct cacgtttttg cataaatttg acttaatacc
     4861 gagaaactga tcccttctgt cataacctct ttttatttat gtctgtttct ttttgaaaac
     4921 aaccattttt tcaacgttta ctctcttgcc aatccaacat catctaacag atacaactat
     4981 ttcaatgatt accattacac taaaaaatcg gaaactcacc tctccgaaca caaatacata
     5041 agactataga atgtttcatt taagaaaaaa agcgtttcat tatcaaaaag gtaagatctc
     5101 ctttaaaatg aaattatctg tagtactaat aatgcagaca attttatgaa tctgaaagga
     5161 gcatctccaa tgaaagcagt cacttggcaa ggaaaacaga aaatggaaat cagaaacgta
     5221 gatgatccaa aaatattaga accaacagat gcaatcatcc gtatcacagc aacagccatt
     5281 tgcggctccg atctgcatct ttaccatcat ggtgaagcag ttttggaaaa agggtatgtt
     5341 gtaggacacg aaccaatggg aatcgttgaa gaagtgggat ctcaagttca aacattgaaa
     5401 aaagggcaac gtgtggttat cccttttaat attagttgtg gtcattgtca tttctgcctg
     5461 aatcacatgg aaagccaatg tgatgaatca aatccagatc agttatatgg cgggcttttc
     5521 ggatttggca agttgaacgg taatcattgg ggtggtcaag cggaatatct aagagttcct
     5581 tatgcagact ctacttcatt tgttgtacca gataatgatc ttcctgatga aaaagtttta
     5641 tttctatcag atattttgcc cacagcttat tggagcgttg aaaacgcagg agtaaaaaaa
     5701 ggcgatacag ttgtgattct aggttctggt ccagtcggtc tattcgctca aaaattcgct
     5761 gttatggctg gagcagagcg cgtaatcgca gtagatccag tacagcaccg tctagataaa
     5821 gcggctaatt acaatggggt tgaaacttat ttcttagaag acacggcaca agctggaaca
     5881 gaattgtatg aactggctaa aggtggcgca gatgtcatta tcgattgtgt aggaatggac
     5941 ggattagagc ctgtcaaaga aaaagcgaaa aatttagtaa gcttacaaaa cggcacaatc
     6001 tctccattac agatggcatc gcaagctgtc aaaaaatttg gaacagtcca agtaactggt
     6061 gtttatatga ctccagcttc ttcttacccg ctgcaagaat tttttatgcg ggatatcgct
     6121 gtcaagcatg gacaggcacc aattatccat ctaatgccaa aaatttacga aatgattgca
     6181 gaaggactat tcgatcctac acaaatcatt actcattcgc tgccactatc agaagcagca
     6241 caagcatatg agatctttga caaaaaagaa gataaaaata taaaagttgt tttgaaacct
     6301 tgaattcagt aaaaaataca ggtgattttc tttatgccca aaatcaaacg aaatacccat
     6361 gagatcgaaa taggaaaaga agaagatatc gaaatacgag gacgtcgctt ccg
//
LOCUS       DACEXQ010000158         6348 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.158, whole genome shotgun sequence.
ACCESSION   DACEXQ010000158 DACEXQ010000000
VERSION     DACEXQ010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.158"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..816)
                     /locus_tag="IXH04_002102"
     CDS             complement(<1..816)
                     /locus_tag="IXH04_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852675.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDEKHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQ"
     gene            complement(819..1175)
                     /locus_tag="IXH04_002103"
     CDS             complement(819..1175)
                     /locus_tag="IXH04_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852676.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(1473..2447)
                     /gene="sgrA"
                     /locus_tag="IXH04_002104"
     CDS             complement(1473..2447)
                     /gene="sgrA"
                     /locus_tag="IXH04_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAQ1852677.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(2643..3482)
                     /locus_tag="IXH04_002105"
     CDS             complement(2643..3482)
                     /locus_tag="IXH04_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ1852678.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            3667..4554
                     /locus_tag="IXH04_002106"
     CDS             3667..4554
                     /locus_tag="IXH04_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAQ1852679.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(5138..5833)
                     /locus_tag="IXH04_002107"
     CDS             complement(5138..5833)
                     /locus_tag="IXH04_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAQ1852680.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(5817..6215)
                     /locus_tag="IXH04_002108"
     CDS             complement(5817..6215)
                     /locus_tag="IXH04_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ1852681.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         2010 a         1258 c          931 g         2149 t
ORIGIN      
        1 ttgaaaaatc aaccgtctgt tcttttcata ataagtatta agctctggaa ttctgcgagc
       61 taccccacta aaaaactgtt gcatgattgg atacatcact ggaaatatat gctgaaaatc
      121 tctgtcttct gctgattttc caaaagcatc caccttcttt tttgtcccaa aaacccttga
      181 atacaaatgg atcattgcca aattgacata tagataattt ttttctgaag ttgtaaaaca
      241 aatcttgaag tttttttcaa aattttctac aatagacgat tcaatccagc cccaactttc
      301 ttgtacttta tctggagtat ccaacaaatc ttcttccaaa taggtgttca ttacaccaaa
      361 cataaaataa aggtaagctt gctctgttgg agtgatgtga ttcatcgacc agaagtttgc
      421 ttcaaaaaaa ggctctagta tgtaatgcgt aaactcttct tttgttatca atggatgcac
      481 aaatttgttc atctcttctg ggacatacgt aatcgagtgg ccttgtttca ttcgttgcaa
      541 cgaaattctt agtaggctga tccatttctt tctatctata ctacgcgtat atggcgcatg
      601 tttcgcaaat ttttctaata gttgacgttt attttttata cttgataata tatcagaatg
      661 cttttcgtcg aatgactgcc aatacatcaa gtgaaaaaag tatcggatac ggtattcttc
      721 tccttctatc ggattacctt ttttcaagct gcaagataaa tgatagtttt tcaaaatagt
      781 attaagttct cttaattttc tttgaatcgt acgcaaactt atataatttt ttgactaaga
      841 ttttctaagg attgataagt acctttgtaa agtgaatcca gtaaaatata accaatcgtt
      901 ccctctaaaa gtatagaata aactttactc agtaaaaact gagaagaaaa ctcgactttt
      961 atcatcttgt tttctgaaag aaaagataat ttatattctg gatacctttc gcaaaacgat
     1021 tctgcttgtt gaatcaccga attcatctta gccagggtgt agtttagatc taaagcgata
     1081 tccttcacca catataattt ctcgtctctt tcataacaat agttcaaaaa ttgatatact
     1141 tgcctttcat tgatatcaat aaattcttca aacaaagtac atactccttt ttatctatta
     1201 tttagactta ttccgacagc tacatgaata acgtacaaat agattcgtta ctgatttttc
     1261 agagatacta ccatgcgcag tgtgcattgt caattattct tgctcttatt ttccatttgt
     1321 gttcctgtta tttcctgttt ctcaaatatg ttagtaaaca aaaatagatc tggcaaagct
     1381 acaacttcgc caggcaatac agcactctcg aatgaaatat tcactcaaaa atctatcgtt
     1441 ttattcaatt ttagatctgt tgcttttatt ctttatttca ctcgatggaa gagagaacga
     1501 aaatagcttg ctcctacaat caaaatgagt aacacaccca tccatgtaat tagcttgttc
     1561 aaggttctac taccagtttc tggtaaatcg ctttttctat tatctgtttc tttcgctttt
     1621 tcttgatgat caattccacg agaaacaaaa tcaggctgtt ttagcacact ttttaggttg
     1681 ctccctgcat ttctcttttg ttccttagtt ggtatgatag gtaccaaatc aagttcatta
     1741 tttacttttc ctgtgctgct ttcgctgctc gtgcttcctg tgctactctc actactcatg
     1801 cttcctgtgc tactttcact actcgtactc tccgtactgc tttcgctgct cgtgcttcct
     1861 gtgctacttt cactactcgt actctccgta ctgctttcgc tgctcgtgtt ttctgtgcta
     1921 ctttcactgc tcgtactctc tgtactgctt tcaccgctcg tgttttctgt actgctttca
     1981 ctacttgtgc tatccgtact gcttggcggt ccaacaactg tccaactgct tggtttcaaa
     2041 ttctccgtga tcgtcatctg tccatcataa aaaacaatca aatcaacttt atctttactc
     2101 ttgatctcat caatcgatag ttctgcagaa atcattattg tatactcaat accagcggtc
     2161 cattgtacgt cttttaaata aacttcccca ataacttttt ctgaacttcc attaccatcc
     2221 caaccatatg ttagaacagc atgtacatca ctagcattca cgcttttgtt tggtttgact
     2281 ggaatagtaa tgacttgttg tgtcatgtca taaacaatcc gatcagtttt cacagtaaat
     2341 gaaagattct catttgcccg ttcatttgca gaagtactga ttccaatcat tgctgcaatt
     2401 atactcagcc caagaataac aaataatacg gtcgctgttt tcttcatttc tatcactctc
     2461 catataatta ccttctattt gataaaggaa ttatataaaa tgaaaatgat gagaacacga
     2521 cagtcagttg tgaaaatcta acaaaagatg tcgtgtattc gcttgaattt gtttactttt
     2581 tcgtttaatc aatgacagac ttaaaaaagc aagaccgata tcagccttgc tttcttcatc
     2641 ttttattgat aaacagcagt gtatttcttc atccgctttg ttttatatgc atagagtaac
     2701 aaaccaacaa tcaagactaa caagaataat gtagttgata tagatgcttc agccccaaag
     2761 tttcccccag taattattgt attttgcgat tggatcagag tggagaaaaa accttgttct
     2821 tgcgttccgc tgactggtat acccaaaata gctccttgtg tccaattcca agtaatatga
     2881 gcaccaatag ccgtaaataa acttcctgta ctgtagataa gtgcgctgaa aaagcaacct
     2941 gctaaaaaga tattgaaaac tgccagaaat gtcatgttag gattcaatcc gtgcaaaaga
     3001 gcaaataatg cagcagatcc aatcattgct attaacggac caaatctaag catctgatgc
     3061 tgaaaaaagc cacggaataa aagctcttct gatgccccca ctgcaataaa taaaataaaa
     3121 ctcgttatca gcggcacaat gtttgcgttt cctgaatcta accgatacgc ccccgtcaaa
     3181 tataatggac tagtaataag cagaaacact ccaattccaa caacgagacc tcccgctaaa
     3241 tacccccagt cttttcctcg aacagaaaca ccataatctt ctattctttt tccttcgacc
     3301 ttactagcaa gaaataaagc tagtaaaaaa acagctaaat gtccgaccgt accggcaatt
     3361 gctccaatcc attcactatt tccaataaac atatccgcaa gtgcattatt gacaagaatt
     3421 cctaaaacaa aaccaactaa gactattaaa acagatccaa tcaaccgaat aatcctgatc
     3481 atctttcatc cctcctttat attaagttta acaacccttt tctttcttgt ctcgtgaaaa
     3541 ctatgtaaat gttctccatc atagagagaa tagattttta cgaactaaga atatctacag
     3601 caaccttacg aaaatgaaag actgtctccc taattcttct gttagactgt ttttaataag
     3661 gaaaggatgg gaaatttttt gaaaaaacaa ctactaatta tgtgcctttc agcatgtgca
     3721 attttactat tctcaggttg tagcaagtcc aatgaaacga ttgcaacgtt taaaggtgga
     3781 acacttacag ttcaggattt ttatgataac ccaaaagtta aatcaaataa tcaaacacta
     3841 ttaaaaaaaa tgattgtctt caaagcattt gatgatgtat acggaaaaga gatttcttca
     3901 aaggaagtaa caaaagaata taatgaacaa atcaaaaaac tcggtccaaa ctacaaggaa
     3961 caattaaaag cagttggcca aacagaagag acatataaga aactctttaa acagatgctg
     4021 gctttccaat acggtttgaa agccaatgta aaattaacag ataaagatct agacactgct
     4081 tggaaagaat tctatccaga agttagtaca cagatcattc tattttcgac taaagaagaa
     4141 gcagataaag caaaaaaaga agcaaatgaa ggggaaaatt tctccaagct tgttcaagct
     4201 tatggaaaaa ataaaacatt aaaggaaact gatggaaaaa tgaattttga ttcaaccaat
     4261 ccagagattc ctactgaagt aaaaaaagca gcatttaaac taaaaaacgg ggaagtttct
     4321 gatatcattc ctgtaacaga tccaacgact tatcagcaaa gctattatct agtaaaaatg
     4381 gtaaaaaaac aggataaagg ttctaataaa gacaaataca aatctgagct agaaaaaatt
     4441 gctaccgaag caaaaactct tgatacggaa ttcatggata aaacaatccg taaagtgatg
     4501 aaaaaagaca atgtaactat taaagatcct tatgtaaaaa atatattcaa ataaacaaat
     4561 ttgacccaat tggggatgtg gcaaaatttg agaagtgatt tttggaaatt ccttcttatt
     4621 tttgaggcaa accacttttg ccacaacttc atcggggcct ttttttattt gtaccaaaac
     4681 aaatcactgg atttgttaca atcatgagga tataatttaa aaaagttttt tatttctatc
     4741 acatgaatca atgcgccatt aagtagattc tttcatgctt ccctattttt tcacttgccg
     4801 tatcgcttca aaaacaacta tcataaactc aaactcgact gaaaagttat tttaaaaaag
     4861 actaaaaata tcccttctgg acttgttcct cttattctgt tatactagag aaacacgaaa
     4921 gatattttta tgcgacactt ttcatttgtt tttatgaaat ttattcaaaa ctggcgtaac
     4981 ttcagagtta caaggatgta ttagaggtta gggaatacat cgtttaaatg agaaataagt
     5041 aatagtcacc caaactttaa attaatgaat attgaatcgt cagatagcct cctcgagttt
     5101 tcgagaaggc tatcgtttat tttatggaga aattgattca gaagtaatca gcgacctttt
     5161 ttagttcacc aatcgtttct gcttggaaag tttgcaccca atcttcttgg aagcccctca
     5221 ctgctttatc aatcgagggc atctctaata cttgatctag tagctcttta cctaacgtta
     5281 tcgcatgagc tccagatcga atggctgtag tgacttgttc acaatttttg aaactagctg
     5341 ctaatatttt tgttggtctt tgcgtacgtt cgatctcagt ccgcaacgaa tcaatcactt
     5401 tcatagcatc aatgccttga ttttgcatcc tgttataata aggtgcaata taatctgctc
     5461 cattattaat agctaaatac ccttgtaatt ctgtgtaaat agctgtaccg gtaatcaaat
     5521 aaccttcttt tttcaacttt ttgatcgctt gaaggcctgc ttgattcacc ggcaccttga
     5581 taaacgtatc ttttcccagt tcttctatga taactcgagc atcctctatc atttcttctt
     5641 ccgttcttcc tattgtctga atatggatag gaatctgtcc gattattttt ttgatttctt
     5701 tcatatgcga gaagaagttt gacgctcctt cttttttcag aattgtagga tttgttgtta
     5761 cgcctgttaa attcaaaaac tgttgacaag aataaatact ttttgtattt gctgtatcaa
     5821 gcatgatttc catatagctt tcccccattc tgatcctctt gatcgatttt aatgattgtc
     5881 ccctttctta aatgcggcat ttccttatct tctacataca ttgttcctgg aagttccggc
     5941 tcatgagatc catcaaattt tatcgtgata tgtcctaatt cattcaaatt agtttggaca
     6001 acatctccta ctgctgtgat tacatacatt tgtttatcaa aacagagctt catatcacaa
     6061 gttatttctt ttcttacctg attcaattca atcttataac aataatctgt taatgtttcc
     6121 ggtgcttcat ctccaaataa aatcattatt ttttctttcc tgaatacttc tgcttctata
     6181 ccaacatctg ttacaaccgt ttcaaagatc gtcatctctg ttcctcctcg taaaatatca
     6241 tttgtctaat agtttttatt caaaatgttt ctagattata aaatttattg aataacacat
     6301 tcctaacgga atttgtttcg atattgtgtg atttctcaga gtacaatg
//
LOCUS       DACEXQ010000159         6257 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.159, whole genome shotgun sequence.
ACCESSION   DACEXQ010000159 DACEXQ010000000
VERSION     DACEXQ010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6257
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.159"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            107..796
                     /locus_tag="IXH04_002109"
     CDS             107..796
                     /locus_tag="IXH04_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852682.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            921..3272
                     /locus_tag="IXH04_002110"
     CDS             921..3272
                     /locus_tag="IXH04_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAQ1852683.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3352..3747
                     /locus_tag="IXH04_002111"
     CDS             3352..3747
                     /locus_tag="IXH04_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852684.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4072..4689
                     /locus_tag="IXH04_002112"
     CDS             4072..4689
                     /locus_tag="IXH04_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1852685.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            4730..5029
                     /locus_tag="IXH04_002113"
     CDS             4730..5029
                     /locus_tag="IXH04_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852686.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5063..5230
                     /locus_tag="IXH04_002114"
     CDS             5063..5230
                     /locus_tag="IXH04_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1852687.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5275..5838)
                     /locus_tag="IXH04_002115"
     CDS             complement(5275..5838)
                     /locus_tag="IXH04_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1852688.1"
                     /translation="MIFKLRNRTEGLNIDKLFQEKASGKDTERPEFQKLLNYIREGDC
                     VIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLDTAMFDMFLSL
                     LSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLVFKTVVDMLKQ
                     GIPVAEIAKENGISRPTIYKIKKANDL"
     gene            complement(5866..>6257)
                     /locus_tag="IXH04_002116"
     CDS             complement(5866..>6257)
                     /locus_tag="IXH04_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAQ1852689.1"
                     /translation="LTLDIWLRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSA
                     FRKLQSVGLYTKTEHRTVKYLNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGI
                     YKKNRRNGTLFGFSVSTEIKVLMGITA"
BASE COUNT         2098 a          907 c         1257 g         1995 t
ORIGIN      
        1 gctatttcga ttaaggatct aaaagagctt ttaaatagta aaagaaatga attaaagcag
       61 ataaaagaaa ctaattcagt ggaattagag taaaggaaga ttcattatga aaaagatttt
      121 tggtgtttct gttgggctac ttatgatgtt aacatttgga aatgcagtat atgctgctga
      181 gaaagaagtt atttcagatc cagagcagta cgcctcacga tactttgctg attttgaaga
      241 agaagataaa gtacttgcat tgccagaagg tggtttttta aaaggggaag ctaaaattgt
      301 atctatggat aatccagata taattcaagg ttcatataat tcagaaactg atcctgcttc
      361 agtttcagtg acagaagcaa aaaaaattgt tgctgaaaaa ttcaaaaatg atattgaaac
      421 tactccaatt atgcctcgag gagcatttcc acccgatgtt ggtactagcg gacaccttct
      481 tgtactagca ggaggaggaa gctataaatc acagccattt agtggtgcgg gatggcgttt
      541 tggaggtatg ggtgtaaaac cagctgatgg tacaggcaac tttttacgtt ggaaatccta
      601 cattgacgat ggaagagtag gaaatgtaaa tcaagccaac gcaactaaat ctggttctat
      661 tcaaggaatg gcattgtatg ctcgtgaagg attcaaatac tttgcaccaa acgggcctgg
      721 ttggacaatg aatataggta tttattacac ttataatcct gttgcaggta cgtattatgt
      781 tgttcaaaat atttaataaa acagcacaaa gagatttctt tgtgctgttt tattattttt
      841 agttgttgac aaacactaaa atataaaata taatcaacac ataaacgaac gtatgttcgg
      901 caagaaagca ggggattttt atggatgaga tggttttaag tactcagaaa tggctaaaca
      961 agaaatacag taatgttaca gggtttgata aggttccaga aaatgggcga acagggtggc
     1021 ctacaatcta tggacttatt gaaggactac aagtagaact tggtattact aatctagtag
     1081 ctaattttgg acctaccact gaaaaaatgt acgataatca ggttactcct aaatggggca
     1141 aaaatctacc taaaaatatt gtatttctta ttcaaggtgc cttttggtgt aaggggatta
     1201 atccaggtgg cttcgatggg gtatatactc catatttaga tactgctgta aaagagttgc
     1261 agactgatgc agggtttaat ggaagcgctg taactggagt tttggatgcg aaatgggcca
     1321 aagcgttatt tgatatgagt gcttttgttc ttgttcccgg cggagatagt aaaattagaa
     1381 aaatgcagca atggttaaat gtaaattatt tagaatacac gggaataatg ccttgtgatg
     1441 gtatttatca aagaaattca aatcaagcat taatttttgc attacaagct gaactaggat
     1501 attctcccag tgaagcaaca gggtcttatg gtaatgggac aactagtaaa acttatcctg
     1561 tgtctgaagg taactcggga aactatgtaa gaataattca atatggatta tatgttaatg
     1621 gttattttga agacggtaca ttcgatggta ttttcactaa atatatgggt ttagaagtac
     1681 tggcattccg aaaatttatg gtattgcctg aatatactga aatagcagat gttgttgtaa
     1741 taaaaggact attgtctagc gctggagata ttagaagatc tgcagatggc gcagatactt
     1801 ctacgcaatt aactagatct caaatccaaa cattggttga taatgatata aaaatagttg
     1861 gtagatatct tactggtact gttggaattg gaaaagatga acgaaataag tatctaacta
     1921 cagaggaatt gaataatatt ttttctaaaa atttatctgt gtttcctatc tatcaagatg
     1981 gaggtgcagc attagcttat tttacttatg atcgtggctt atcagatggt aagaaggcta
     2041 tagatgcagc aaaaaatcta aatataccat taacaacagt tatttatttt gctgttgatc
     2101 tagacatgtt aggggaagaa attcttgcat atgctggaga atattttaag ggggtatctg
     2161 ctgtaatgtc atatagcggg tatcaaacgg gtgtttacgg aacaagaaat gtttgttctc
     2221 aaataatcaa taacgggtat gcttcgtttg catttgtttc agatatgtcg actggttata
     2281 gtgggaattt aggttttcct atgcctagac aatggtcttt tgatcaattt gttgaattta
     2341 ccataggaag tggcaacgga gctgttggta tcgatttaat cgctacatcg ggtagagata
     2401 gcggatttaa tgagttatca aatgatacta ataatgataa ttacattgcc aaatacaatc
     2461 agaaggttat aaatcagatg gtacgtgcgt atcaatatct atcacaagct ggtcttgata
     2521 atccttggaa ccctcatttg acattttata gatatgtaaa ttattctggt ttgtcatggg
     2581 acatcatttc ttctcctgta acagagcatg atagaaaaat ttatgatgaa tataggaata
     2641 aattaactaa tagcgagggc ctgtataatt attttataga tcctaatact ggttcaataa
     2701 taggtttgcc tcacttaatt gtgacgttac aatcacagat gtttctagcc gacacaatca
     2761 atgatagtgt ttctgacttt gcaggttggt taggagattt aatgacttgc tggggtgaag
     2821 ttaagaaatt aggaatacaa atggagcaag gtgtatttgc attagtaggt acggctgcag
     2881 gtggaacatt tagtctagaa gatttatacc aagatgttga cgcggtaaat gtttatacct
     2941 tagcaaaaag aaattctgat tcaacagctt tatcggcttt aaaaacgtac tattgtgata
     3001 agggtgctat tgatagattt aatcaatttg ttaagaacag atttggttct tcagataaaa
     3061 tcgcttcagt aacaaatagt ttattagaaa atgacgaaac agttgatatc ttaggtgtag
     3121 ggataatttc atttttcatt gataaaatag ttaataattc acttgggaca gattttactt
     3181 atcttgattt tattggagat gaggggaaag ttgcaaatgg attttctgaa aaaattattc
     3241 aatttcaaga aaatagaagt aaacttaatt aagaatcgtt tactatttaa acagtgtcct
     3301 tttccttttt aaatatatac gataaaataa gtatatgaat tgtaaaagga catgatgatt
     3361 atggaagaaa tagaatggaa taatctgact caaaataata aaaataattc tggatgtgga
     3421 attatcttta tgtttgttat agttttggga atagtagtac ttttaataac tgtcaagata
     3481 gtagattact ttgcgaatac tcctgaagaa caaatacaga gaaaacttcc tgagtttgaa
     3541 agagtaccac cttatgatcc aagagataat tcatttcaag taaaaaaatt aaaagagtct
     3601 ccaaatgaaa tgtggtttga agttacagtt gaatcaagcg acttcaaaga aaaaaagaaa
     3661 gaatacaatt atattgtaaa ggcaaaagta tcaaaaagat ttatttttaa ccctaaatta
     3721 gaaatatata gtgttgtaat agaataatag tgttaaaaaa attgtaaaag ttaaagaagt
     3781 caaattctga tgtgaaaaat aaaatatacc atggaagaca taaaagaaaa actggataac
     3841 tagtttattt aatggcagac cgttaaggcc tgccattttt atgtatttta gcggaataaa
     3901 tataatcatg cgggagtgta aacttactgt ttcaaaaagt atactttttg acatgccttg
     3961 attttctttg tttatgtatg gcataataac cacataatac aatttgaaac gtttctgatt
     4021 taatctgatt gttttgaaac aattttagtg aaaaaagggt agataaaaac gatgagaaaa
     4081 tttttttata tccgggtcag tagttatgat caatcgatcg aacgacaact gatttccgcg
     4141 aaggaattag gaatccctga agaatatata tttgtagaaa aagccagcgg caaagatttt
     4201 aaacgccctg aatatcaatt aatgaaacgg atgtttcgaa aaggagattt actctatcta
     4261 caatcattag atagactagg cagaaataaa caaatgattt tagaagaatg gcgagaatta
     4321 actaaggaaa agcagattga tatttctgtg ttagacatgc ctctgttaga tactgctagt
     4381 tacaagcaac tagatgggtt agagacgctt atctctgact taattctgca attactcagc
     4441 tatatggccg aagacgagcg gaaacgaatt aatagccggc aaagagaagg cgtagaagct
     4501 gcgaaacaaa agggtgtaaa attcggcaga aagaaaattg ctttacctcc tgaatttcct
     4561 gcgatctatt ctgactggaa agcaggaaag ataactgcag tctatgctat ggatcaattg
     4621 ggcatgaaac gaaatacctt ttatcgacgg gtgaaagaat atgaacatac cttgcaccac
     4681 ttttcttgat agatataaca gctattagac gggaagggag agtttgaaaa tggacttaac
     4741 gaatcaaagg gtcgttttta ctggaacgct gcagcaaatg acacgaaccc aagccatcgg
     4801 tttggttcac tctttaaacg gccattgtca atcaagtgtt acttcaaaaa caaatttcct
     4861 ggtttgtggt cagtattcta agtcgttgtt tgatgattct ctctatacaa aaaaggaaca
     4921 aaccgcgcgg gacttgattg cgttaggtca tgagattacc attttatcag aagtcgaatt
     4981 ttatgcgtta attagtcaac agttgaaaga atggcaacgg tatttataat cgttagtaaa
     5041 atttacaagg aggaaaattt ttatgaagcg aaaatcttat gatcgttggg gcattgaaat
     5101 aactagtttt aatcgaaata ttgcttttca cgtggagtgt gactgtgggg aattagcaaa
     5161 aagtgtttat caaaaaaatt acttttactg tcctaaatgc catcgccgat acggggtaca
     5221 tggaacatag aaacgaaata gtgcggtaga aaagcgaatg aatgtagtat atttctacaa
     5281 atcattcgct ttctttattt tataaatagt tggtcgagag atcccgtttt cttttgcaat
     5341 ctctgcaact ggaatgcctt gctttaacat gtccaccacg gttttaaaaa ctaagcgttt
     5401 ttgcggatct gttgagttaa tagaatactc gattggtcgt cccttatatc ggccggcttt
     5461 ttgagccaat aatactcctt gccgctgacg ttcacgaatt ttctcacgct cgttttgtgc
     5521 aatataactt agcagtgata aaaacatatc gaacatagcg gtatccagga gttcatttcc
     5581 agtattaaaa tctaaaaact gtgcatctaa tatttttaac gctacttttt tctgtttcat
     5641 aaaagcaact gtatttttta tatcttcata atctcttcct aaacgatcta gtgatgtaac
     5701 gatgacgcaa tcaccttcac ggatataatt tagtaatttt tgaaactcag gacgctcagt
     5761 atctttacca ctggcttttt cttggaataa tttatcaatg ttaagtcctt cggttctgtt
     5821 gcgaagtttg aaaatcaaac gcgccctctc tgaactccaa atatcttagg ctgttattcc
     5881 cattaatacc ttgatttcag tagacaccga aaagccgaag agcgttccat ttcttcggtt
     5941 ctttttatat attcctcgaa tggtctccat gcccttaatc gtggaagagg ctgtacggag
     6001 actttgataa aatttatttc gtcgtttaat aggtcgatgg tcttgttcta ttaaattgtt
     6061 aagatacttc acagttcggt gctctgtctt agtatataaa cccacactct gtaactttct
     6121 aaaggcggag ccaagagaag gtgctttatc ggtcacaatt gctttcggct caccaaactg
     6181 tttatggagt cgttttaaga aagcataggc tgcttgcgta tcccgtttct ttcgtaacca
     6241 gatatctaag gttaagc
//
LOCUS       DACEXQ010000160         6194 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.160, whole genome shotgun sequence.
ACCESSION   DACEXQ010000160 DACEXQ010000000
VERSION     DACEXQ010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.160"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..696
                     /locus_tag="IXH04_002117"
     CDS             <1..696
                     /locus_tag="IXH04_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAQ1852690.1"
                     /translation="MYEQDIDDKYAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKK
                     QYPFFQECAEGTFDDIPFDKFYRSLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFN
                     EGVDVAELPEIWNQKYEEYLGIRPTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQL
                     LHSLQQEIDVENVLASDDYSPIKNWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLID
                     YMKDLYFNVYQVK"
     gene            777..1460
                     /locus_tag="IXH04_002118"
     CDS             777..1460
                     /locus_tag="IXH04_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1852691.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            2105..3295
                     /locus_tag="IXH04_002119"
     CDS             2105..3295
                     /locus_tag="IXH04_002119"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAQ1852692.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            3397..4350
                     /locus_tag="IXH04_002120"
     CDS             3397..4350
                     /locus_tag="IXH04_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAQ1852693.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            4419..5768
                     /locus_tag="IXH04_002121"
     CDS             4419..5768
                     /locus_tag="IXH04_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1852694.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(5951..>6194)
                     /locus_tag="IXH04_002122"
     CDS             complement(5951..>6194)
                     /locus_tag="IXH04_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852695.1"
                     /translation="TTIHYLGFILLIISGFMLVFNLQTWQQALLEMDKMTPHALSKVL
                     GYPVYLIWLPIFISGCLVLLNIYRGVRRIVQLRKSK"
BASE COUNT         2032 a         1054 c         1323 g         1785 t
ORIGIN      
        1 atgtatgaac aagacatcga tgataaatat gcgtatacac cggtttatga aggtacatca
       61 atgggtatcc atgaatcaca atcgttgttt aacgagatca ttattggaag tagtcgcagc
      121 ttctggaaaa agcaatatcc tttctttcaa gaatgtgcag aaggaacatt cgatgatatt
      181 ccttttgaca aattttatcg ttcattgaac catacaaaat caagtttggt tcgtatcgag
      241 gcagacagtt tgacttaccc tctccatatc atcattcgtt atgaaatcga aaaaatgatt
      301 ttcaatgaag gggtagatgt tgctgaacta cctgaaatat ggaatcaaaa atatgaagag
      361 tatctgggaa tacgaccaac aaatgatgtg gaaggcatcc tacaagacgt tcattggtca
      421 ggtgcaagct ttggttattt tccctcttat gcattagggt acatgtatgc ggcacaatta
      481 cttcattctt tgcagcaaga aatagatgtg gaaaatgttc tagcgtcaga cgattattct
      541 ccaatcaaaa actggatgtc agagcatatc catcaatatg gtgcatctag aaaaccaaat
      601 caattaatca tggatgcaac acatgaaccg cttaatccgc aatacctgat cgactatatg
      661 aaagacctgt attttaatgt atatcaggta aaataaaata caatttacaa agatagctga
      721 aattcatcta atctatagat gttttcagct atttacttaa gaaagaagtg gaacgaatgg
      781 ataaattaca agtgatcgct gattatagta tggaccaatt agcgcatgat caaacaggac
      841 atgggagcga tcataccaaa agagttgtca agttagctga aaaaattttg gagacggaac
      901 cgcaggcaga ccgctttgtg acgcttgcag cagcttacct acatgatacg attgatgata
      961 aagtcgttaa aaatgagaac gaggcaaagc aacagcttcg tgtattcctt cgctctttgc
     1021 caatagtaga ggaacagatc tcgatgattt tttccattat cgaaaatatg tccttttcta
     1081 aaaatctctc tgaagcggcc gaactatctc tggaaggtaa gatcgttcaa gacgctgatc
     1141 gtatcgaagc attaggagca atcggcattt tgcgtactgc ttatttcggc ggtggacatg
     1201 ggcacccgat ttttgatcct gaactgtatc cgcaaacatt caaggacaaa gaaaattacc
     1261 gaaaaggcac gacagtgatc aatcattttt atgaaaaact gttcttgctg tctgataaaa
     1321 tgaatactga ttacggatat gaagaagcca aacgaagaga aaccttcatg cgtgaattct
     1381 tagaagaatt ttttctggaa tggtctattg acgatgaaaa ttttactcca gacaattgga
     1441 tcaaaaataa caaaaaatga tggaggttgt gacagaagtg gtcagctcca agatatttcg
     1501 gatcattttc cgtaaaagtt acttctggaa cactgtttac caagaggtcg tgagcctgcc
     1561 gttcagctcc gagtcacaag gaacaatttc aaaaaatagc ttctcgtgtt tgatgaatat
     1621 tgtaacttgt gattcggaat tggcaggaga acaccgttta ttcggaagaa taggactgtg
     1681 acaagacttt tgtcacagtc cttttttcga attcaacaag taaaactttt aagaaatatt
     1741 cagagagtaa aattaaaaat tgctgataaa cgaagatgga aattttatgt atttttattg
     1801 gtctatgcaa taaataaatt aaatattcag aatacaattg attttaacga aagggcttct
     1861 atcaatggtt gtgttgtatt tatctgccca ggtagagtaa aatagatcat tatctaaaag
     1921 gtaattgttc atactatata agtcatttga aagtaaaaag atactgtaaa taaaaaatgt
     1981 ggttaaataa ttctcataat cagtgtaagc gttatcaaaa atatagcata cttgctttgt
     2041 agatgaatga cacgatatat gtttggtatg ttctgcaaga cagatgaata tggaggtgga
     2101 gacaatgtct tccaaagaac taacaatgta tttagagaaa gaattggatc aactgaaaga
     2161 aaaggggtta tacaatacga tcgacatact agaaagtgaa aatggtgctt gtatcatcgt
     2221 agacggaaaa aagatgatca atctagcatc caacaattat cttggatttg ccaatcgcga
     2281 agaattgaaa aaagcatgta ttgaagcaac cgaaacttat ggtgtaggag caggagcagt
     2341 ccgaacgatc aatggttcgc tgaaaatcca tcagcaatta gaagaaaaaa tcgcagaatt
     2401 caaagggaca gaagcagcaa tcgcctttca atctggattc aactgcaata tgggcgctat
     2461 ctctgcagta atgacaaaag aagatgctat cttatcggat gaattaaacc atgcatctat
     2521 tattgacggt tgccgcttat ccggagcaaa aatcatccga attaaacatc aagatatgaa
     2581 agaccttgag aaaaaagcaa aggaagcgat agaaagtaag aaatataaaa aaatcatgta
     2641 tatcacagat ggtgttttct ccatggatgg agacattgca cgattgccgg aaattattcc
     2701 gattgttgaa aaatatggac tgatcaccta cgtcgatgat gcgcacggct caggcgtaac
     2761 tggaaaaggt gcaggaaccg tcaagcattt tggattaagc gacaaaatcg atatgcaaat
     2821 gggcacgtta tcgaaagcta taggtgttgt tgggggttat gtagctggat cgaaaacact
     2881 aatcgactgg ttgaaagcta gaagtcggcc attcctattt tctacttcgc taactccagg
     2941 tgcagctgca gctgcattgg cttcgattac tttgatgcaa gaacatccgg aacttgtcga
     3001 aaaagtttgg gaaaatgcta attattttaa agaagaattg aaaaaagtag gctataacat
     3061 cggtatgtct gaaacaccta ttactccagt cattttggga gacgaaaaag tgactcagac
     3121 tttctctaag aagctgatcg aacatgggat ttatgctaaa ccaatcgttt atccaactgt
     3181 gcctcttggg acaggcagga tcagaaatat gccgactgcg gaacatacga gggaaatgct
     3241 tgatgaagca gtagcagttt atcaaaaaat aggaaaagaa atggaaatta tttaactaaa
     3301 tttagttttt tcgtctattt aaaattagag aacatgttca cgcatatatt gttaatcatc
     3361 tgaagatagt agtaaaaaag atcagggagg atcatcatga aaaaagtatt ggtgacagga
     3421 tgtttaggac aaattggttc agagttgatc acacggctac gtcaagaact aggcagtgaa
     3481 aatgtcattg cgacagatat acgcatgcct gaagtcattg agaaaaacga tatctttgag
     3541 atcctagatg taatggatta cgaaaaaatg cgcagcatag cagaacaata tcaagtggac
     3601 acattgattc atttggctgc tcttttgtca gctgttgcag aagcaaaacc acaattagcc
     3661 tggaatttga acgtgactgg gctggtcaat gcattggaag tcgctagaga attccagctg
     3721 aaatttttta cgccaagttc aatcggcgcg tttggtccaa atactccaaa agatcatacc
     3781 ccacaagata ctcttcagcg tcctacaacc atgtatgggg taacgaaggt agctggagaa
     3841 ctactatgtg attattatta tgtgaaatat ggcgtagata ctcgtggtgt ccgtttccct
     3901 ggattgattt cttatgcaac cttgccagga ggcggtacga cagactacgc agtcgatatt
     3961 tattattcag caatcaaaga aggaaagtac actagtttca ttgatcaagg tacatcaatg
     4021 gatatgatgt atatgccaga tgcgatcgat gccattataa aactcatgaa tgcagatcct
     4081 gatcgattgg ttcatcgaaa tgccttcaat atatcggcga tgtcttttga cccggaagag
     4141 atcaaagcat ctattcaaaa aatcatacca gattttgaaa tggattatga tgtggatcct
     4201 gtacgtcagt caatagcaga atcttggcca aattcactag atgtttcttg tgcgcaagag
     4261 gaatggggct tttcgccgaa atacgactta gatgtgatga caaaggatat gttggaaaaa
     4321 ctgaaagaaa aattggtaga gacggtataa aacaagaacc acatccaaaa agttttttat
     4381 acttttggga ttggctatgg tatgaaaggg gacaagggat ggaagaatat caagccacgc
     4441 cagtaaaaga agtaaaaata gaaaatgaat taaaacgtac gatgggattt tttactgctt
     4501 tatcgactgt tatgggcaca gtgatcggtg caggggtatt ttttaaagcc gcaagcgtag
     4561 cagaagttac aggatccgcc agtttgcata tgttttcttg gtttctcggt ggtatgattt
     4621 ctgtctgtgc aggactaaca ggagctgaac tggcagctgc tattccagaa acaggaggga
     4681 tgattaaata tatcgaacgg atttacggaa atacagcagc ttttttattg ggctgggcgc
     4741 aagtcgtgat ttattttcca gcaaatgtag cagccttgtc gatcattttc ggtacacagt
     4801 tcgtcaatct atttggtctt tcccagtcaa tgattgttcc cgtagctgtt acagctgctg
     4861 tatcgatcct gttgatcaat tttttaggtt ctaaagctgg aggggctttt cagtcgatta
     4921 cgcttgtctg caagcttatt cctttgtttg tgatcgtcat tttcggtttg ttccgacaag
     4981 aaggagtaga cttccaactg tttccgatcc aagcaggaga aaatctttca tttttctcag
     5041 ccttgggagc gggcttgcta gcaacgatgt tcgcatacga cggttggata cacgtaggaa
     5101 atatttcggg agaattgaaa aagccggcga aagatttgcc aaaagcaatt tctttaggaa
     5161 tcatcggaat catgatcgtt tatttattag taaatgcggt gttcttaaga accgcatcta
     5221 tcgacggagt cgcaggaaac agcaatgcgg caagtgacgt tgcaaaaatg atttttggcg
     5281 gcttcggtgg aagactggtc acagttggta tcttgatttc tgtttatgga accatcaatg
     5341 gctataccct tactggaatg cgtctccctt atgttatggc aaaagaaaac aacctgcctt
     5401 ttagcaagct ctttgctaaa cttcatgata aaactaaagt tcctgtagcc gcaggtatct
     5461 tagagctagt catcgctatt ggcatgatga tgatcggtgg ttttgataca ctaacggata
     5521 tgctgatctt tgtcatttgg attttttata cgatggtctt tgttggtgtc attcttcttc
     5581 gtaaaaaaga acctgatttg tttcgaccat acaaagttcc aatgtatccg tttattccac
     5641 tagtagcaat cattggaggg acttttatcg tatcaagcac gcttatcact cagacctttt
     5701 tagcttcgat ggggatagta ttgacacttg ctggtatccc gatttatctc tatttaaaac
     5761 gtagatagct aaaacattcg aattttatta gaactgcact tctctgccaa acaaaatatt
     5821 ttatttaaat gaggagtacg tggaagaaat gtcaaaagta ctcgttaatt ttgatcacgt
     5881 aaatgtcgtt ttgtctacga tgaaatagca aagtaaaaga cagaaatcgg gctcaaccga
     5941 tttctgtctt ttactttgat ttgcgtaact gaactattcg gcgtacgccg cgataaatat
     6001 ttaataaaac caagcaacca gaaatgaaaa tgggaagcca aatcagatag acagggtagc
     6061 caagcacttt agacaaagca tgtggagtca ttttatccat ttcaagaagt gcttgctgcc
     6121 aagtctgaag attgaagact agcatgaaac ccgaaataat caggagaata aatcctaaat
     6181 aatgaattgt agtt
//
LOCUS       DACEXQ010000161         6177 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.161, whole genome shotgun sequence.
ACCESSION   DACEXQ010000161 DACEXQ010000000
VERSION     DACEXQ010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6177
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.161"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..816)
                     /locus_tag="IXH04_002123"
     CDS             complement(<1..816)
                     /locus_tag="IXH04_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ1852696.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHH"
     gene            complement(913..1191)
                     /locus_tag="IXH04_002124"
     CDS             complement(913..1191)
                     /locus_tag="IXH04_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852697.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            1459..3468
                     /gene="metG"
                     /locus_tag="IXH04_002125"
     CDS             1459..3468
                     /gene="metG"
                     /locus_tag="IXH04_002125"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAQ1852698.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(3947..4534)
                     /locus_tag="IXH04_002126"
     CDS             complement(3947..4534)
                     /locus_tag="IXH04_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852699.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            4709..6031
                     /locus_tag="IXH04_002127"
     CDS             4709..6031
                     /locus_tag="IXH04_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1852700.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
BASE COUNT         2011 a         1098 c         1233 g         1835 t
ORIGIN      
        1 atggtgcccc ggcttgagct gatagatttt tgaaggacgg ccgattccta caggacgttc
       61 accgatctca tcgaaatatt ggagcatagc tttcttaaaa ttggaatgat cgatcgtttt
      121 ataatcgatc cctaagaatt tagcaaatac ttttcgagct tccgtaatcg tgaaatcttt
      181 acctaaaact tgtagaactt gcggctcgtg ttccatttta ttgactacac ggttgaatgc
      241 tttcaagatg atttgactat ggtcaaaagc caaagtatct ttgccagaag actctccagt
      301 gactaaatca agcgagatag ccacttctcc gctagataaa tggatcttgt tactatgacg
      361 ttctagtgtg aaccagcgta cttccttggc atcatctccg gcgattagcg gttcttctcc
      421 aataaaggct aagtagctga ctgtgacgac ccagcctcgc ggatctcgat taggcgtact
      481 gaatgtatgc agttgctcaa tattttcttt tgaaataacc acaccagttt cttcctttgt
      541 ttctcttaag acgctttctc cagttgactc atccttttgg acaaagccac caggcaatgc
      601 ccatgaattt cgataaggat gcccttttct ttggatcaac aaaactttca gctgatcttc
      661 ttctttgtta tagcacatca gtacgatatc gacagtcaaa gagggcttgt catattctgg
      721 acgttcctgt ttttgatacc agttaagaaa ttcttcttca cttgctttat gttcataata
      781 taatttttct tcttctttag aagcaaaacg gttcatctga ccacctccat tgacagtaaa
      841 agtacttttt accttattgt agcagactct gaaaaaaaac caaggattta cttgaaaaat
      901 ctaggttatc atttaaatat gaaaaataaa tggaagtatg agattgaata aaaagaaaag
      961 cagataaaac ggcaatactt tataccgctc ttcacgaatg gcaaagaagc cacaaataac
     1021 gatatatacg tcaatgataa aacgcaaaat ccaagctgct gtcataaaac tgttcaaatg
     1081 cccatggagg ttaaaataac catacatacg aatataaaaa tagctgaata ttaaatgtgc
     1141 taatgaaaga aaagcaatac taagtgtaaa aaaaagatgt ttcttctgca tgattgttcc
     1201 agtcccttac taagatagat agtctttatt ataccacgtg tgaggggaga aataaaaagc
     1261 cgttttttag tagatatgaa acaaaacaaa aacccgcatt tattcaacct attcatcctt
     1321 ttaaaggata gattgaacgt agatagaagt cgtttatcca gtctttttca ttttgaaaaa
     1381 aagctaacag catgcttttt ttatggtata ctagcaagga aacgaattcg catcatgcga
     1441 taaaggagga agagccccgt ggcagaaaag gaaacttttt atatcactac gccaatctat
     1501 tatccaagcg gcaaacttca tatcggcaac tcgtatacta cgattgcatg tgacgcaatt
     1561 gctcgttata aacggttgat gggctttgat gtgttttatc tcactggagt ggatgaacat
     1621 ggtcaaaaaa tcgaaacaaa agcagaagaa ctaggtgtaa aaccgcaaga atatgtagat
     1681 aaaatggcgg ctgatgtcaa gaaattatgg aaaacgcttg atatcagtta tgataaattc
     1741 attcgcacga cagatgatta ccataaagca gctgttcaaa aaatatttga tcgtttatta
     1801 gaacagggag atatttactt aggtgaatat gaaggctggt actctgtttc tgacgaagaa
     1861 ttctttacag aaacacaact tgcagaagtg tacaaagatg acgaaggtaa cgtgatcggt
     1921 ggtaaagcgc caagtggaca cgaagtagaa ttggtaaaag aagaatcata cttcttccgc
     1981 atgagcaaat acgcagaccg tctattggaa tactatgaag aacatcccga attcatccag
     2041 ccagaatctc gtaaaaacga aatgatcaac aactttatca aaccaggatt agaggatctt
     2101 gcagtttcac gaacaacttt tacttgggga gtaccagtca agagtgatcc gaaacatgtg
     2161 gtgtatgtat ggatcgatgc attatccaat tatattactg cactaggata tggctccgaa
     2221 gatgaaagtc tattcgaaaa atactggcct gcagatgttc agatggtcgg taaagaaatc
     2281 gttcgctttc atacgatcta ttggccaatc atgctgatgg cccttgattt gcctctacct
     2341 aaaaaagtat ttgggcatgg ctggctattg atgaaagacg gaaaaatgtc taaatcaaaa
     2401 ggaaatgtcg tttatcctga aatgcttgtg gaacgttatg gtttagatgc actccgttat
     2461 tatttgcttc gtgctgttcc gtttgggtca gatggtgtct ttacgccaga agattttgtt
     2521 tcccgtgtca attatgatct ggcaaatgat ctagggaact tattgaatcg aacgattgct
     2581 atgatcaaca aatattgtga tggactcgtt cctgactatg cctcacttgt cactccattt
     2641 gatagtgaac tgtctacgac ggctgctaac gttgtcggac gttaccatga tgcaatggaa
     2701 aaaatggaat tcaatacagc attggctgaa atctgggtgc tgatttctcg tgcaaacaaa
     2761 tacattgatg aaacagaacc atgggtatta gcaaaagacg aagaaaagaa agctgaattg
     2821 aatagtgtca tgatccattt agctgaatct ttgcgtatcg cagcgatatt acttcagcca
     2881 atcatgacag aaacgccaga gaagatcttc aatcaactag gattggactc tgaaacaatg
     2941 aatcttgaag gattacactt tggcgaattt ccttcaggaa caaaagtagt tgcaaaaggt
     3001 acaccaatct tcccacgttt agatatggaa gaagaagttg cttttattca agaaaaaatg
     3061 tctgaaggta ctcaaacaaa cgaagataca gtcaaatggg atccagaaga aacagaactt
     3121 gtttcaacga aagaaaaaca aatcaaattt gatgtatttg aaaaagtaga actaaaagta
     3181 gcagaagtca tcaactgtca aaaagtagaa ggggcagata agttgctgca attccgacta
     3241 gatgctggag acagccaaga ccgtcaaatc ttgtcaggaa tcgctgaatt ttatcctgat
     3301 ccaagcgaat tgatcgggaa aaaagtcgtc attgtagcaa atctgaaacc acgaaagatg
     3361 cgtggacaaa tcagtcaagg aatgatcctt tctgcagaag caccagatgg ctctttacaa
     3421 gtaatcgaag caccaaaatc catgccaaat ggctcagaaa tcgcatagaa attcaaaggg
     3481 ggtatgacac tagttgtgtc ataccccctt tttccgaaat ttgagaagca attttcgtaa
     3541 attccttctt attacttgaa gccaatcata taatacagaa acagagaact atccaaaata
     3601 aatcctagaa aaagaagtac gagcttcgct gatttttgtt aatttagaat aatactaata
     3661 ggaatcgtaa aaacagaaaa aaagcataat gataaagggt cgggcagatt ttaattgatt
     3721 catctggtac tcttttttat tgtccttaca ttcataaagt ttgaatgcaa tgactttatt
     3781 acatctatat ttatatctaa cagtagcgtt gatgtgttaa ttataagttc tatcacttta
     3841 tgttcaatta gtttgtgctc ttatatgtgt gattgaaaat atagataaaa aaacaatcat
     3901 cggctttgac ttcgatgatt gttttcttta ttttgtaaaa atcctatcac ttgattttat
     3961 ggtaaccttt tatttttgtc ttcaaacggc ttggtaagag agcagtaagc agtgcaaatg
     4021 ggatcgaaaa gacataccag aaaatcgtac gtccgatacc ataatgttga tagagctttg
     4081 tcaacatcac gtgtaacaag atcaacccta aaagcaagga gccagctata aaccaatgat
     4141 tatcccaatg catgacttca ctaagtgcca tcactccaaa aggatagaaa taaaaataga
     4201 tcccgactaa ggtcatccgc cagttctttg taaatgttcc gatattcaat aaaaagaaaa
     4261 tcgctgaaag caaaataccg ataggcggaa aaagcccaac caaaaaggaa tacaacatcc
     4321 cccacaagat catcacagga cctttgatca atgcaacgat tgcgatgaac aaagtcagca
     4381 atattagttt ttgtgataac gttgcaacag tcattaatcc aaataaaatt aatgtgaacc
     4441 aatgccaatg ggactgctgt tgttctttac gggaaaaatc gacccggttg gataaataat
     4501 ctgctaattt cggataagaa cgcttaaaat ccataaactg ttcctccttc gtggaaaagg
     4561 taggtttagt atacgaaaaa taaaaaagaa atacatccgt cttaagaggg agtttttcac
     4621 tgaaagtgga gaaaaaagaa tggtaaatta aaatattctc agttatactg aaaattatac
     4681 caaatttcaa agaagaaaga ggatgggaat gaaaggcaaa ctaaaacgag aaatcaatct
     4741 gtttggcgca ttagctaccg ttatgggaac cgtgatcggt gcaggcgtct tcttcaaaac
     4801 tgctgctgtt actgctagta ctcagtctac aagtttgaca ttgctagctt ggcttttggg
     4861 tggtttctta acgatctgtg ctggattgac agtggctgaa ttagctacag caatcccgga
     4921 aactggtgga gctgtaaaat atattgaagc tgcgtatgga aaattgccca gctttttatt
     4981 aggatgggcg caaagcttga tttactttcc tgctaatatt gctgcattat cgattatctt
     5041 tgcgacacaa ttgacgaact tattacaatt atctaccgat tatcttttgt tgattgcagt
     5101 gatcaccgct gtttctgtga cagggttgaa tttgttaggg acaaaagtcg gtgcttctgt
     5161 acaatcagtg acgttgattg ttaaattaat accaattgct gtgattgtca tatggggatt
     5221 attgacccca ggacaaggaa ctgtccagtt atttccaatc gaagctggaa aagatgttac
     5281 ttttgtcgaa ggattgagca gtgcgttgtt agcaacgttg tttgcttatg acggctggtt
     5341 aggtgtcggt gcaatggcag gggaaatgaa acggccggaa aaagatttgc ctaaagcaat
     5401 tattcttggt ttgagttttg taacagtcgt ttatctattg atcaattttg tttttttgaa
     5461 aactttgccg attgatcatc ttgccggtaa tttgaatgca gcttctgaag catcagacgt
     5521 catttttgga ggaatcggtg gaaagctggt cactattggg atcttgatct cggtatacgg
     5581 tgcattgaac ggttatacac taactgggat ccgcgtgcct tatgcgatgg ctttagaaga
     5641 cgagctacct ttcagtaaac agctgacaaa gctttcaaag aagtttacgg ttccttatgt
     5701 tcctgcggtt ttccagttag cagtagcttg tattatgatg agccttggtt ctttcgactt
     5761 tttaacagac atgttgattt ttgtgatgtg gctattcagt ttactgattt ttatcggtgt
     5821 ctttatttta agaaaaaaag cgccggaatt gccacgtcca tataaagtac cactttatcc
     5881 aatcgttccg attattgcga ttctcggtgc tattttcatt ttaggtatga cgatgatgac
     5941 gcaaacgaaa ttagcattga tcggtatagg tgttaccttg ataggtatcc cagtctatta
     6001 tcaaaaaaag aaaaaacgct cagaagagta aaatatttca acaacagaca gaaaaagtac
     6061 gaactaggtc ctctagatct tcgtgctttt tctatttcaa taaaacaagt tcttctattt
     6121 tgatttttgc tacttctgca agtagtgctt atgtgctaaa gtaagaatga aaaggag
//
LOCUS       DACEXQ010000162         6131 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.162, whole genome shotgun sequence.
ACCESSION   DACEXQ010000162 DACEXQ010000000
VERSION     DACEXQ010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6131
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.162"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(63..1268)
                     /locus_tag="IXH04_002128"
     CDS             complement(63..1268)
                     /locus_tag="IXH04_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ1852701.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
     gene            complement(1291..1444)
                     /locus_tag="IXH04_002129"
                     /pseudo
     CDS             complement(1291..1444)
                     /locus_tag="IXH04_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(1447..2832)
                     /locus_tag="IXH04_002130"
     CDS             complement(1447..2832)
                     /locus_tag="IXH04_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAQ1852702.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            complement(2861..3247)
                     /locus_tag="IXH04_002131"
     CDS             complement(2861..3247)
                     /locus_tag="IXH04_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ1852703.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            complement(3263..3577)
                     /locus_tag="IXH04_002132"
     CDS             complement(3263..3577)
                     /locus_tag="IXH04_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ1852704.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            complement(3930..4409)
                     /gene="rlmH"
                     /locus_tag="IXH04_002133"
     CDS             complement(3930..4409)
                     /gene="rlmH"
                     /locus_tag="IXH04_002133"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAQ1852705.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYGLAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            4837..6126
                     /locus_tag="IXH04_002134"
     CDS             4837..6126
                     /locus_tag="IXH04_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ1852706.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVMMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
BASE COUNT         1773 a         1243 c         1154 g         1961 t
ORIGIN      
        1 aggttttgtc caaaattcat aaaaatgttc tcctctctat attcaatgaa tttgtatttg
       61 agttattttt ttgttgttat cacgtcctgt tcttttactg actgttgctt caaaatctgc
      121 ttgtgtcggt ctgtcagttt cgcatggtcg agaatgtctt ttacaacctg cgtctggttg
      181 atttcatcaa gtttaatcgc aacctttaag gtcggggcaa cttgatgaga tagccagttc
      241 agcgtccttt ggaaggagta aggctctggt tttgtggtta gttttaatcg ttcacgattg
      301 ttcccaataa accaagccca ttcttcattc agtttccaat cagaacgagg tttggaatcg
      361 tctttatcta caaaacggat ataccgattg ataattttaa aggcggtatg ctctggattg
      421 tcatagacga gtaaatcacg gactgcataa taggcacgct catttttcaa tcgaatctca
      481 aaacggtttt ttacttctgc gtcttcaatg ggaatatcat ttttcttgta ctgctcgtag
      541 tccttttcat agatacagaa ataaacttca ctttgtaatg aaccgatata gagggtgttt
      601 cccatacatt ccttttcctc tttgcgtacc agttcgccac tgcgatagct tttaaaactg
      661 cggaagacgg agatacattc ttcctgttgg cacttttcag tgagtacagg gatatttaaa
      721 atccctgtct tatcgttaat ggcaaggtca aggcgtttca tcacaccgcc agccaccaaa
      781 acgtccataa agaactcata ccagcttctt tgttgtgcca gaagatagct ttcaaattgt
      841 ctgcacccac gacctttcaa ttccaccaga actcctttgt ccagttcatg ggagcaaagg
      901 acgaatatgt cgcctaaagc ataatgctct gaataagaat agaaaccata gtcctcatga
      961 agaaaatagg acagtttcag ttgtaagatg ttttcgacca cctgctgtac gtctgttgtc
     1021 ggaaagcgaa ttcttacata atcaaacagc atttcaaggg gagcgtcggg attgaagcgt
     1081 tccagagctt cccaaaggga ctgctgtaaa tcctctgatg gcttgacttt tcctgtttca
     1141 atatcgctta gatactgcct tgtaatacca gtcgcaacag ctaaacggtt ttgagatagt
     1201 ccataagcca agcgtttttc ttttaaatgc tgtaaccaag tttgttcatt cagtaaaaat
     1261 ccctccaatc aaaaaggcgt atgtcaactt ttaaagccca tttgacatac gctgaaattt
     1321 tgtaaatccc ttgtaaccaa aggattttct aatgtttttt tgactgtttc ctgtcgattt
     1381 gtacccccct gttagatacc gaggggttaa gtgctggcgt ggctattgcc acaccagcca
     1441 gcaagatcag tccacacctg cgacttccgc ttcgcacgtc gcctgcgtgg actgtctgct
     1501 gttggataac tttttaattt cctccaagaa atcatatcct tttggtacaa ggggagtata
     1561 aaactctgat atgacacttg ttcctacatc aacatagcca cgacctttga ttcgctttaa
     1621 gaagaaatcc ttttgtacgt cactgccaaa catcatgcca tagcccattt cagacatacg
     1681 acctaaagcc actctgaaat taaactgatc acggattccg tcgcctaaat attttgcgtc
     1741 tggacgttga caagccagta ttagaaagaa gccagcttga cgacctaaca tgacaatctg
     1801 tttcagctta ttcataactg cggtgttttc ttttgttccc agcatttcca tgaaagcgac
     1861 gtattcatca aagattaaga agtgtgccgg gagacctaag taagcataat ttttgccagt
     1921 cttatagttc ttcatctgct tcatttcctc actacgtttc atcatttctt catagaatgt
     1981 ttcaatgcaa gaaagcaagt cttcttttct atagtagaca tttgccatca cagaacctaa
     2041 gtccgcaagg tcagcatttt tcgggtcaag aatatacagt tttgaatctg tatgaagcaa
     2101 ggcttcaatc agtgtcagta taaagtaagt tttaccgcca cctgtaccac cagcaatcaa
     2161 catatgaggg agcttatcat attcccacca tacgtttttc attaagcgaa gtttaccatc
     2221 tttagcttct acttcatcaa tagaaatacg actggctatg gtgtcataga gcaaagtata
     2281 ttccacatag gaatccttta actctttatc cgtcagctca cagtacaagc cactctctaa
     2341 tttcttttcc aagtgtaaga gttggtcttg atattttccc agcgtgattt ccacccgtat
     2401 ctgtatcaag ccatttttaa gtcgataata cattttaggg aagtaggtta tcttttcctt
     2461 tgtacgacca gcactatctt taaagaaacc ctctgttttg acctgttcag attcatacca
     2521 cttgttttca agtatcatct ttgccagttt ttgacggtgg taaagttgtt taaccgtatc
     2581 atagcgaacc cgtttgaata caaacgctac cagcaagcag ataagaattg cgacactgaa
     2641 actgataatt aaatagggaa tgtcaatctt atctgcttgt gataggttaa aatcctgcca
     2701 gttgatctgc tggattgtct tcacatgaaa cagtccgaca accagcagga aaacaggcag
     2761 gagtgacgct atcgtaaaat gaaagactaa atctttacca gatgggcgaa tccttttacc
     2821 acgctgtttc atgcgaaaaa gtctcctttc tacctagcga ctatttgtct tgtgtcggtt
     2881 ctttctttgc ttgtggttga gctttgaatg aactagaatc ctttgtcagc acaatatcgt
     2941 ctgccttgat ataccagtca acatctgctc cttgataggt ggcagtagca acggtgtccg
     3001 caatgggatt gataagttcc acccgtgcgt tataatcaaa ctctttcaaa ggcacgctgg
     3061 caggaatact tacttgaatc atgcgtcctt gtcctttgga ttttaagtca taggtacgtt
     3121 ccttgatttc atctgaaacc gacccgtctt cattttggat tctcacttca cgacgtagag
     3181 cagagaattt caattctcca aaagtcgtgt ctttatctaa tacaatgcca tttgctaatc
     3241 tcatcatttt tcctctcttt ctttattctt ttatcatgtc gtcagcatgt aaaaggtaat
     3301 ttgtaaaacc acgagtgccg attttgtagc cctctgcggt aatacgtgga ttgactaact
     3361 tcacacgttc ctcaaagccg aaatgttttt cgccagcttc agcaggaagc accaccacaa
     3421 tatcatctgc tctttgaaca tcagaataga gattatagct tcttgataag acagttagcc
     3481 gtccgttgat tcttcgctga acgactttat cctcgccagc aaattctaaa ttgccgaatg
     3541 ttttttccat gttgggaatc acaaatttaa gttccatatt tttacctatc ctttcttttt
     3601 tattggctga atgaatgttt gatggtctta aagagtgggg aacgaccttt tgattcttga
     3661 ttttttgttt tcataagttc acttcctttc aaaatcgggt aaaaaaatag acacctcatt
     3721 ttttgaagtg tctacctatt aaatattcaa attttattgg aagtatcttt atatcttcac
     3781 ttttcaagga taaatcgtcg tatcaaagct cattcataag tagtaaatta gtagtaaatt
     3841 gagtggtttt gaccttgata aagtgtgata agtccagttt ttatgcggat aactagattt
     3901 ttatgctatt tttctcttaa aaaatatcat tatttgtggt atggagcccc cgcgttgatc
     3961 cggaaggcac ggtagatttg ctcgacgagg atcaggcgca tcaattgatg tggatacgtc
     4021 attttgccaa aagagatctg agcattgctt cttttcatga cggcttcgct gagtcctaaa
     4081 gaaccgccga taacaaaaac aaggtggctt tttccttgga tacctaattg gtcgatttgt
     4141 ttagcaaaag attcgcttgt gggatttttc ccttcaattg ctaagccata gacgtattct
     4201 tgttctttga ttttcgctaa gatccgttct ccttcttttt cttttacttg tctcatttgc
     4261 gcttcgctta aattctccgg ggctttttcg tcaggaactt cgatcaattc gattttcgca
     4321 taggcattca gccgtttgac atactcattg ataccttgga tcaagtattt ttcttttaat
     4381 tttcctactg aaataatttt gatattcatg ttttctctcc tttgatcttc acgctcactt
     4441 tgtttattat aatcaatttt ttcgtctata tgtttatttt gactattttt gttaaaaaga
     4501 tttccccaac agttatgcac aacatccaca ggtttatcca cagaaaacag cgttgtttta
     4561 ccttatttca tcacattcat taggcaaaca tacgttttat aagaatttat ccactttata
     4621 cacaggctgt ggatatgttt tatcccttat ttttccaatc ctatccgcgt agttttacac
     4681 aatatctgtg gaaaaagaaa atcaaatatt ttcatctgaa aacaatgatt tttcctaaag
     4741 tttttcataa aaagttcaaa gtctcaaagt agactaaagt caacaaataa gactatacta
     4801 acagtagata aatgaatgta attggaggag gaacacatgg atagaaaaaa tgtgacacca
     4861 aagatgaaaa aaaataaaaa taatagcctc tggcgtaagt tgggtctggg gcttgttgga
     4921 gggatcgttg gaggactact gacagctggc attttttatg cagcgatggg cactggcaac
     4981 aatagttcta ctgctacaag cggcaatcaa aattctgccg gtgaaacagt tgtggaaaat
     5041 gtcaaagtca atgtagattc tgatatcacc aaagcggtag ataaagttca agacgctgtc
     5101 gtttctgtta tcaacttaca aagtcaaaac caatcaagcg gttttggcgg attattcggg
     5161 cagcaagaag aaagtagcag ctcagatgat agtaatttgg aagcttatag tgaaggaagc
     5221 ggcgtaatct acaaaaaaga cgggaatact gcttatgtag tcacaaacaa tcacgtagta
     5281 gatggacagc aaggtttgga agtcatgatg aaagacggta cgaaggtaaa agctgaatta
     5341 gttggaacag atgcctattc agatttagcg gtgttgaaga tcaattcaga caaagtcgaa
     5401 acggttgcct cttttgggga ctcaagtgct ttgaaggttg gtgaacctgc gattgcgatt
     5461 ggttccccat taggatctga atatgcgaat tctgtcactt caggaatcat ttcttccttg
     5521 aaccgacaag taacaagtac gaatgaatcg aacgaaacag tcaatatcaa tgcgatccaa
     5581 acagatgcag cgatcaaccc gggaaattct ggtggtcctt tagtgaatat cgaaggacaa
     5641 gttatcggta tcaactcaag taagatcgca agtacttctg aatcgacttc taatgtaagc
     5701 gtagaaggaa tgggatttgc catcccaagt aacgatgtag tgaatatcat caaccaactg
     5761 gaaaaagacg gaaaagtgac acgtccagct ctaggtatca caatggtcga cttatctgcc
     5821 gtttctactc agcaacaaga acaaatcttg aagattcctt catctgtaac taacggtgtc
     5881 atcgtgacaa atgtccaaac agctacaccg gcagaaaaag ctgggttgaa acaatacgac
     5941 gtcattacta agatcgatga caaagaagtt tcttctggtg tagaacttca atctgctttg
     6001 tataagaaaa aagtgggaga ttctgtcaaa atcacttact atgaaggctc gaaagaaaaa
     6061 acaactactg ttgaattgac gatcgatcaa tcggcattaa aacaaaatca aaatagtgaa
     6121 aattaataaa a
//
LOCUS       DACEXQ010000163         6081 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.163, whole genome shotgun sequence.
ACCESSION   DACEXQ010000163 DACEXQ010000000
VERSION     DACEXQ010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6081
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.163"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..354)
                     /locus_tag="IXH04_002135"
     CDS             complement(<1..354)
                     /locus_tag="IXH04_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAQ1852707.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQ"
     gene            504..1550
                     /locus_tag="IXH04_002136"
     CDS             504..1550
                     /locus_tag="IXH04_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1852708.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            1552..3705
                     /locus_tag="IXH04_002137"
     CDS             1552..3705
                     /locus_tag="IXH04_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ1852709.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(4178..5629)
                     /locus_tag="IXH04_002138"
     CDS             complement(4178..5629)
                     /locus_tag="IXH04_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1852710.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(5626..>6081)
                     /locus_tag="IXH04_002139"
     CDS             complement(5626..>6081)
                     /locus_tag="IXH04_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ1852711.1"
                     /translation="KTITLKEELDFCEKYVYLYQMRYPNRVAYHFMIDPDLEKIEVPK
                     FVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKDNGKGMTEKRLKQIEEKL
                     SHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLTVSITFKEG"
BASE COUNT         1784 a         1208 c         1096 g         1993 t
ORIGIN      
        1 ttggtggcca gcaccgttag cttcttcgtt gaaggcattc gcatatggat cgatattgat
       61 ataaaaaaat tgctttttga ctaacccgct gatcatgtca gctaattcct catcttcttt
      121 tgcaatcact aaataaggac gaacttgtgc agtagagtct cttaaccaca tcgctggaat
      181 gtctcccgtc aataaaaagg tggttccgtc attttgcttt ttgactgttg tcaacagagt
      241 attggcaaat gctgctgtaa aattctttgc ccagtccgca tggatatccc cacatttttg
      301 tgtgatatct tccataaatt tctgcaccga ttttggtata tctttataag ccactaataa
      361 aaccttcttt ctatttgata tatctttaaa gattatactt catacagcca attatgtcta
      421 cgctttcaaa aaatatcttt ttatttactt tttcatccat tatggtatta tttgaatatg
      481 atatatcatt aagaggtgtt tatatgaatc aaccattgta ccagacaata tttaatgatt
      541 tgcaaacggc tattttaact ggtgaattac ctgtagacag ccaactacca actgaaaaag
      601 aactttcacg tacctataaa gtaagcagaa tcacttccaa acgtgcattg actgaattag
      661 agcaattagg acttatttat cgcgttcgcg ggaaaggcag tttcgttaaa tccgcttctc
      721 cggaacagat tcttgcttct cctacaaaaa cacgacggat tctgtttctt cttccatatg
      781 tcgctgattt gtctgttggt gattttagca agggattgaa ccctgtgatg caagataaac
      841 agattgatgt catgatgacg acgcttgact ttctacaaaa taaaacagca aaagagctaa
      901 tcaatgaatt tgacggattg atttattatg ctcttgatac agaacaacat cttgacttac
      961 tttttgagct ttcattgaaa gaattccctg tcgtcatatt agataaaaaa atctatgagt
     1021 taccttttcc taccgttctt tccgataatt tccaaggcgg tgcgcttgct gcctcttatt
     1081 tgatcgaaca gggacattca aaaatcgctt atttgtttgg tacgcagact catccacaat
     1141 ccgttcgaca gcgttacctt ggttaccttg aagcattgga caaagctggt ttgactttcc
     1201 acacttcact ggatgacaaa agcgcgacat tagatgaatt gctttcttat atcactaagt
     1261 atgaagtaac tgcttttgtt tgtgaaaacg atgttgtcgc tatccagacc atgaatctct
     1321 tgagacagca tggttatcat atccctgaag atttttcaat tatcggattt gatgatatcc
     1381 aagcagcagc tcttgttgac ccgccactga caaccatcgc acaagatttt gaacagttag
     1441 gtaagcttgc gggagatgct ttggtcactt ggttagagac aaaagatcga ccatccgata
     1501 gtaaatttcc agtttcattc atcaaaagac aatcaacaaa ggagaaataa aatgaatatc
     1561 ggtcaaatag atactagaca tgcaacagcg aatcagccaa atttctctaa tgggaactgc
     1621 cagccttata ctggcgttcc ttttgggatg aattattttg caccgcaaac tacggatcaa
     1681 aacggcagct ggtggtttca tcctgatgac cgcgtttttc aaggctaccg tcttacccat
     1741 caaccaagtc cttggatggg tgatttcagt catatgctgt ttacgccgat caatggaaaa
     1801 ctacaggaga acaccctttt ccatgctcaa agttcttacc gcccagaaga aagtatattt
     1861 tgcccgactc atctgtcgat ctgtcaatta cgttatggca tctgttctac attgatacct
     1921 agcatgtatg gcggaatctt atctattgat tacagcaaaa atgacagcgg tttactttta
     1981 tcctttccgg gacgccatca gctctttgtt atagatcctt acacagtgga aggaaaaatc
     2041 acacattttg ctggttcaga agatcctgat ttttcttttt attttatatt gagatttgaa
     2101 tatcctttaa caattgatga gttagctgtt tccaacaaaa atagcgactc gatcccgttg
     2161 tattttgaac agacaaaaaa acaaactatc cgatttggga catcttttat cagctcggaa
     2221 caagcagctt ataacctgga actagaacaa aaccaaagca aagagactta ccttgctcac
     2281 agtcaagaac aatggcagtc ctattttgat cgtatacagc tcacacacca tgatcaacaa
     2341 caattgcgga cgttttatca taatttatat cgggtgtttt tatttccaca aacattttat
     2401 gaaattgatc ctagtgggca aaaaatccac tatgatacga ctagtcgatc tataaaacct
     2461 ggtgttctct atacaaataa tgggttttgg gatacgtata aaacggttta tcctctttat
     2521 tctttgattg ctgtagaaaa atacgaagaa atgctggaag gttttttgaa cagctataga
     2581 gaaagtggct atttgccaaa atggctgtct cctgatgaac gtggacttat gcctgggaca
     2641 ttgatcgatg cggtcattgc agatgctgct gtaaaagata tccgacgaga cttgatgcct
     2701 gaatttctaa cggcaatgga aaaaggtgct acggttcaaa gcaaacatcc aaattatgga
     2761 cgccaaggaa cagaagatta cttgacatat ggctatgttc ctaatcacta ccacgaatca
     2821 gtcaatcaca cattagacta tgcttatagc gatttttgta ttagccaagt tgccaaggta
     2881 ctagaagaag aggaaatgac gaagaaatat cagcagcaat cattgaacta tcaaaatatc
     2941 tttgactatg agtcaggatt tatgcgttca aaagataaag atggcaagtt cagagaacca
     3001 ttttcagcta atcgttgggg acaagattat gcagaaggaa gtgcttggca aagcagcttt
     3061 gctgtttatc acgatttttc aggactgatc ggaaaatatg gtggcagtga gagatataaa
     3121 gaaaaactca ttgagctgtg caatcagccc ccaacgtttg acccaggagg atatggtttt
     3181 gagatccacg aaatgagtga gatggccgcg atagaatttg gacaattagc tatttcaaac
     3241 cagccaagtt ttcactatcc ttacttgttc agttatatag gacaacctga aatggcacag
     3301 ccactgctta aacagttgat gactcagacc ttcgacgatt ctccaatggg ttaccctgga
     3361 gatgaggaca atggaagtat ggccgcttgg tatatcttca atagtctggg attttatcct
     3421 gtcacgcctg gaaccggtga atatgttatc ggtatgccat taattgacaa ggctgttttg
     3481 cttttgtcaa atggaaagga attgacgatt gaagcaacgc ctaatcggcc gcagcaacaa
     3541 tttatccata aaatcaaacg gatggacaaa gaatatacga aattatattt tacccatcaa
     3601 gatttgctga aaggcggcac gatctcttac caattaggga tcatccctca ccctaagaaa
     3661 tatcaagcaa aagatttgcc tttttcctta tcaaactata attaacaaga ggttgtgaaa
     3721 aagctgtcct gcatcaagta ataagaacag ccaagcaaaa atagatcctc atatttttcg
     3781 tttggttggt cttattaccg cagatgcaag cttttgaaca ccgtttattg agaggttgtg
     3841 aaatcagcgt tctgacctga gtattagaga gacaaatggc aaaatagttc ctcatatttt
     3901 gccatttttt gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaggtcg
     3961 tgaaaaagct gtttagtttc gagtattaag aaaaattttg tgaaaatggc tttccacatt
     4021 tttgcataaa tttggcttaa taccgagaaa ctggcttttg aactccgttt atcgagaggt
     4081 catgaaaaag ctgccttgcc tcaagtaata agaacagccc aaaattagat ttctagacaa
     4141 ttttttataa gttatttcat tacattggct ttttctttta tgattctcca ctgttttctc
     4201 ggtactcttt aggggatctg ccgacgattt tcttgaataa tttagagaaa tacgtggtgt
     4261 tgttgtagcc aatctcactt gctatctcat tgatgttttg attactgtgc aaaagcagtt
     4321 gtttcgcttt ttccatacgt aaatggttca aataagctga aaaacttttt ttcgtttctt
     4381 ttttgaacag ctgtcctaga tacatgacat ttaaatgcag gttttctgac acttctttga
     4441 gtgtaagtgg ttcttgataa tgctggtgca ggattacgat tactttttcc acattgtcac
     4501 tgtacttttt ctgtctctga tgctttttga tgaatgacag aagtagctgc tgaagttctt
     4561 gcaccgtttt tgcctgatgg attttttcga ttccttgtaa atattcctca tcttccagat
     4621 caatcaattc ccgttgaatc tccataaaca gtaaaaaact ggaatatcgt atatcttccg
     4681 gcatcatggc tgctaactga aactgttcaa aaaaatcagt gatcattttt tgagcgactt
     4741 ccaattgttt attttgcagc acgcgtctga aacttgaaaa atcgatggtt tgatcttttg
     4801 tttctgcttg tactgcgtaa aaaacatgct cgtctttatc tccataaaat tgatgcaact
     4861 gtagattgtc tttcgcttgt tggaatgttt cagggatttt gtccaaagaa tctgtctctt
     4921 ccccgatact gatcagccat tcctgatcag gaaagttttc ttgaacaaaa caacagaagc
     4981 catcaacttc atctgcctcc aacaacaacg tgatcaatac acggtctccg taatttcgtt
     5041 gatagtaaaa gatttgttgc cgttcttgca accatctatt gataagagta tcatgagttc
     5101 ttgccagttg aatcaatact atcctatgct gcttctcact tatttgtcct attatttctt
     5161 cttcacttat ttcatccaat tcttcattca accattgact gaataacagt tcttgataga
     5221 tttcttgttg atttttttgt tcattttgct gatctaaccg tgttttgatt ttcttgaggc
     5281 tttcaaccaa ttcgaactta ttcacgggtt tcatcaaata attcacggcg cctaattgca
     5341 tacctccttt caaataatcg aattcttggt atcctgataa aatcataaat tcaaaattat
     5401 gacgttcttt ttgagccgct tcaataaatt ccagtccatt tatttctggc attgtcacat
     5461 ccgttactac taaatcaatc tgtcgctgtt caagtacagc tagtccttct ttggcattct
     5521 ctgctgttgc tactatttga aagcctaaac tttgccaatc aattatcttt tttaacccat
     5581 tcaaaatcat gtattcatca tctaccaata acacttgata cacgctcatc cctccttgaa
     5641 tgtaatagaa acggtcagtc cacctgtctc attattctca agcgacatat aataactgct
     5701 gccgaagctg gcacgtaaac gttcattcac gttttgcaga ccgatagaac cgtgaagttc
     5761 cactttagga tgagacagtt tttcttcaat ctgttttagc cgtttttcag tcatcccttt
     5821 tccattatct ttgatgatga tccttacccg tttgccttcc tgcaagactt tgacacttag
     5881 cgcattatcg aatcgagtaa aatcaatacc gtgtttaaaa taattttcaa ctaatggctg
     5941 gatcacaaat ttaggtactt cgatcttttc caaatcagga tcgatcataa aatgataggc
     6001 tactcgatta ggataacgca tttgatacaa atagacatat ttttcgcaaa agtccagttc
     6061 ttcttttaaa gtgatcgttt t
//
LOCUS       DACEXQ010000164         6042 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.164, whole genome shotgun sequence.
ACCESSION   DACEXQ010000164 DACEXQ010000000
VERSION     DACEXQ010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6042
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.164"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(76..1170)
                     /locus_tag="IXH04_002140"
     CDS             complement(76..1170)
                     /locus_tag="IXH04_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ1852712.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1359..2120
                     /locus_tag="IXH04_002141"
     CDS             1359..2120
                     /locus_tag="IXH04_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1852713.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2235..2675)
                     /gene="fabZ"
                     /locus_tag="IXH04_002142"
     CDS             complement(2235..2675)
                     /gene="fabZ"
                     /locus_tag="IXH04_002142"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ1852714.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            2895..3650
                     /gene="fabI"
                     /locus_tag="IXH04_002143"
     CDS             2895..3650
                     /gene="fabI"
                     /locus_tag="IXH04_002143"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAQ1852715.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(3760..4773)
                     /locus_tag="IXH04_002144"
     CDS             complement(3760..4773)
                     /locus_tag="IXH04_002144"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAQ1852716.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            4938..5858
                     /locus_tag="IXH04_002145"
     CDS             4938..5858
                     /locus_tag="IXH04_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAQ1852717.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         1847 a         1205 c         1083 g         1907 t
ORIGIN      
        1 cctataaaaa aacaaaataa aaaagcagaa ataactggct atttctagcg gattatttct
       61 gcaataacta attatctatt ttttattttc aattaattta aagcttgacc atggtttcaa
      121 aaagtcaagg aggaataatt cgtcatcgga gattcttcct acaacattga cacgtccgtc
      181 gtttttcata tccttcaagg cgatttgtgt ttctcctttg tattgaccgt acccttcatt
      241 gtcaatcagt acatccccat gatgcatatc tcgcgtatta tgaggcggga aatctttatc
      301 tttgtagtat acacgagtca tcgttgaacg aaggatatat tctgagcgat cccctcgata
      361 actatgaagg ttatcaaaca agcagattcg ttcgttttct gtaatacctt cttccgtatc
      421 tactttcaat gttggataat ccgcgttaaa tgcttctgcc atttctttca attcttcttc
      481 acttgcgtac gcatttccaa tagaaatatc atcaatcaat cctgtaagga ttaaatgctt
      541 cacttgtgtc gcaatttcaa gatctcggtg gtcttccagc gtgcacaagc catcttgcgt
      601 aggccaaggg ccaaactcag cagattgcga attgacgaat gccatcgtat tcaaattata
      661 ttttctgaat ttttctgaac aaaaaacaaa gtgttcgtat cctaaaccag agtatcgatg
      721 aggataaaaa ttatgagaac ctaataaatt atccgtgttt ggcgaataac tcatgatgtt
      781 gtcaacgtaa ttcgtgcccg aactcatatt gatctcgatt ttgataccaa aaggattgcg
      841 cgtcattttt gcttcttctg ctcctgtgaa tccgatatct aagcggaccc cgtaggcacc
      901 catcttatgg aaaaaagata agtcatcgta agaaatctcc aactgttcaa ataacgctgg
      961 attgatatca accattactt ccatgcctag gctattcgcg taatcaacta cttctttaaa
     1021 ttctgaaaga actttttctt tatcatcatt gatctgcagc aaactggtaa aaacccgttt
     1081 atacccatac ttatgagcta aatccaagta cgctttgtcc ttttcaaatg ttgaccgttc
     1141 tggataaatc gagattccta attttcccat attaccattt cctcctgcat cttttcgttt
     1201 ttgtatccac ttacattata gcaaacacaa agtaaatggt ctacaccttt tctttttctt
     1261 ttaaaataaa tgataaattc caataattta agtaggtatt ttccctctat tttgttatca
     1321 ttaaagataa caaaatagta aattggaaag gaacagttat gaagaaaaat aaagttattt
     1381 taagtattag tttagcagca gcattgctgt ttgctgctgg ttgttctgca aacaacacga
     1441 cttcttctag ttcgtctaca gaaagttcaa aaagtacatc tactgcggaa agcacgactg
     1501 ccagtagcgt cgatttgaac gcattggaat taccacaatt gacgaaagat gtcgccgaaa
     1561 atgaagatct ggtcgaaatg gtgacgaata aaggcacaat caaaatcaaa ttatttcccg
     1621 aacacgcacc aaaagcggtt gaaaacttta tgacacatgc aaaagacggt tactacaacg
     1681 gactgacttt ccatcgcgtc atcaaagatt tcatgatcca aggtggcgat cctaaaggcg
     1741 atggcactgg aggggaaagc atttggggag aaggatttga aacagagatc aacaaccacc
     1801 tttataatat aagaggtgcg ttatctatgg ctcgctcaca agatcctaac agtaacggca
     1861 gccagttctt tatcgttcaa aatacagatg atatgcatga tggtctttta aaagacgact
     1921 atcctcaagc aatcatcgac gcttataaaa acggcggtta tccttcacta gatggccatt
     1981 atactgtctt cggccaagtg attgaaggaa tggatgtcgt ggatgctatt gcaaatatcg
     2041 aaacagatag ttcagacaaa ccaaaagaag aagtaaaaat cgataaaatc aatatactgc
     2101 aagaagcaaa acaaaaataa aaaaataacg tctgaatcct gcatagatga caacgtatgc
     2161 taggattcag gcgtcttctt tttacataaa aagcaagtaa gcttcctttt gttttgctat
     2221 atcaggtttt atagtcaagc attttggttg acgatgaacg tgaactcgca ctcacagact
     2281 tttttatcat ctacatatgc tgcagataac gctgtaccga ctgatccctt cattttggtg
     2341 atttcaaaat gacatttcag cacatctcct ggaacaactt tttgacggaa tttggcctta
     2401 ttgatcccac cgatataagc tctttttcct agaaactctt ctgattttag gataagtatc
     2461 gaacctgctt gtgccagtgt ttctagaatc aacgcacccg gcatcactgg attgtcagga
     2521 aagtgacctt ggaaaaaatc ttcattgatt gttacatttt ttgtcgcaac gatctttttt
     2581 cctggttcta aagtatctac ataatcaata taacaaatag gataacgatt aggaatcaat
     2641 tccattactt cagtagcaga taatacgtaa gacataatta aactccttct ttcaggcaat
     2701 tgccattttc attactttga ctatcaaagt atcatattcc taaatattta gcaatctttt
     2761 tttaatttaa gaaaaaatag tttgacaatc aaaacatttt gactaataat taagaaatca
     2821 agaagttatt ttagctatta ttctctattt ttttacgact atcaaatttt tatcagaaac
     2881 aaaggagagt cattatgtca tttctagaag gaaaaaaaat cgtcattatg ggcgtcgcta
     2941 acaaaaaaag cattgcttgg ggttgtgctg aagcaatgtt gaaacaaggg gcagaaatta
     3001 tctatactta ccaaaatgaa cgaatgaaaa aatcattagt caaattggtc ggcgaagaag
     3061 cattcactgt agaatgtgat gtttctagcg acgaaagcat cgaaaaagca atgaatgcca
     3121 tcggtgaata tgctggggag atccacggcc ttgtccatgc agttgcttac gccaacaaag
     3181 aagaattgtc cggaaatgtg tctgatattt ccagagacgg ctatcaatta gctcaagata
     3241 tcagcagcta ctctttaatt gctgtatcaa aatacgcaca accgtttctt gcaccaaaca
     3301 gcgggatcgt tagcatgact tatctaggtt cagaacgcgc aatcccgaat tataacatga
     3361 tgggtattgc gaaagcttct cttgaagctg ctatccgtta tttggcagct gagttctcgc
     3421 ctaaaggaat tcgggtcaat ggaatctcag ctggggcaat caaaacatta gctgtcacag
     3481 gtgttaaaga ctatcaaaaa ctgatccagt tatctgaatc tcgtacgcct gatcagcaag
     3541 gtgtgacgat cgaagaagtc ggtaatgctt gtgctttctt aatcagccca ctttccagcg
     3601 gaatcatcgg tgatattatc tacgttgaca aaggtgtcca tctttcataa acttttgttg
     3661 cattctttaa agtaaaacat ataaaaatca ggaaaaaact ttttaaagtc tcttcctgat
     3721 tttttctttt tacgtttttt tcaactcatt actttgaaat tattccacga cttcaatcca
     3781 gccttctggt gcttctatgt ctccaaattg aatacctgtc aattcatcat acaaccgttt
     3841 tgttactggt cctacttccg tttcactata aaatacatga agattgttat tgtgataaat
     3901 cccaccgatt ggagaaataa cagccgctgt accacatgca ccagcttctg caaattgatc
     3961 tagatcatca atgaaaatat ctccttcaat tgcttgcagt cctagtcgtt cttcagctaa
     4021 ctgcaacaag gaatatttcg tgatacttgg taagatagag ggcgattgcg gagtgatgaa
     4081 ttttccgtct tttgtaattc caaagaaatt tgctgcacct acttcttcga ttttcgtatg
     4141 tgtggctgga tcaagataaa cacaatctga gtaatccagt tcttttgctt ctgttcctgg
     4201 aagtaaactc gctgcgtaat ttccgccgac ttttgctgca ccagtcccct taggagcagc
     4261 acgatcgtaa tcagatacaa taaaattcgt tggttttagt ccacctttga aatacgctcc
     4321 tactggcgta cagaagacga caaataaata ttctggagct ggatggacac caatgttttc
     4381 accgacccca atcagtaaag ggcgcaagta taatgtagcg cctgttccat agggcggcac
     4441 aaaatcttca tttgctttta ccacttgctt gacagcatca acgaacatct tttcagggac
     4501 cattggcatc agcaatcgtc tagcactttg attcatccgt cgactatttt gttcaggtcg
     4561 aaagagattg accttgccgt cttttcgacg ataagctttt agaccttcaa aacattgctg
     4621 accataatga agcgctggag agccttcatg aatcgtcagg taattatctt ctgtcaattc
     4681 tccttcttcc cattgtccgt ctttccattt ggcaataaaa cgccaaggag ttttgatata
     4741 agaaaaacct agattgttcc aatcgatttg catatactta ccaccttcgt tattttctcc
     4801 acattttatc attatccata ttatttgtca ttttattttc agcttttttc cttcattata
     4861 gttatttttg ctatttcctt ttttgaaaac ttcttggtac aatacagaaa gaatcaaatt
     4921 ttagaaaaga ggattttatg ttttcattat tagcaactgc cggtacgaca gactcttctg
     4981 gcgagacatt gacagatcaa gctgtcagca aagtaaatgc gtttcagcgg ttttgggacg
     5041 ggattgattg ggacgccatt ttggctgcct ttatccaaaa aagtttatat ctgatttttt
     5101 tgatcgtcct tttttggatt cttcaccgtg tgggaaaata tctgattgat aagtctttta
     5161 atcagtattc taaaaaagcc attctaaacg agagccgctt aaagacttta catagtcttt
     5221 taaatactat ttttcattat acccttggtt tcttttttat ctatgcaatt ttatctgcta
     5281 tcggtgtgcc gatcggatca cttttggctg gtgccggaat tgcaggtgtg gcaatcggtc
     5341 ttggtgccca agggttcatg agtgatgtta ttaccggatt ttttattatc atggaacaac
     5401 agatggatgt tggggactac atcaagctag caaacttaac gattgaagga accgtcgtat
     5461 ctgttggtat ccgtaccctt cagctgaaag caacggatgg tactgtccat ttcatcccga
     5521 atcgaaacat caccacgatc agcaatctct ctcgtgcaaa tatgcaagta cttttagata
     5581 ttcggattgt tcctgaagaa ggatatgaca aaatctatga gatcattgat cgagtaaatc
     5641 gtacattagc tgaaaaatac caagaagagc tccaaactga accttctatc tttggattag
     5701 tggatcttgg aaacagcaat tttgcgattc gtacggtctg ctacgcattg aacggaaaac
     5761 aatatgcctt aaaagaagag ttcttgagct cctatgtcaa agaattgaca gcttctggtt
     5821 tcacgattcc gaacagtcca attgctgtcg gaaaataatt tggacaaacg atgcaacgaa
     5881 tctcactaaa aagagcgtgg gacaaaagta aagaacactt ttgtcccacg ctctaaacac
     5941 gtataaacgg cggaagcaga agcaactcct tcggaaataa gctgaaattc acaaaaattt
     6001 gaaaagcaat tttcgtgaat tccttcttat ttcttggagt ta
//
LOCUS       DACEXQ010000165         6034 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.165, whole genome shotgun sequence.
ACCESSION   DACEXQ010000165 DACEXQ010000000
VERSION     DACEXQ010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.165"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..309)
                     /locus_tag="IXH04_002146"
     CDS             complement(<1..309)
                     /locus_tag="IXH04_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852718.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLA
                     D"
     gene            complement(818..1600)
                     /locus_tag="IXH04_002147"
     CDS             complement(818..1600)
                     /locus_tag="IXH04_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852719.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1665..2501)
                     /locus_tag="IXH04_002148"
     CDS             complement(1665..2501)
                     /locus_tag="IXH04_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1852720.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(2519..3928)
                     /locus_tag="IXH04_002149"
     CDS             complement(2519..3928)
                     /locus_tag="IXH04_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1852721.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3942..5765)
                     /locus_tag="IXH04_002150"
     CDS             complement(3942..5765)
                     /locus_tag="IXH04_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1852722.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
BASE COUNT         1844 a         1310 c          965 g         1915 t
ORIGIN      
        1 atccgctaag acgttgaaaa gatactctgg tgcaattgca tatcccaaaa tatcaacgat
       61 tgtggcaatc agatcttctt ttgattcttc atctgaaagt aattcacgat acaacattgt
      121 ttgtttgctt actgtgtcat attcctctaa ggattcatct gctaaaagaa ccacttgttc
      181 caacaatcga tcggataaat atttatagaa aatcagtccc aataaataat ttttatattc
      241 tgacgcatcc attttactac ggagattatc tgccgcacta aataattttt gatttaattc
      301 tgcacccaaa aagatacact cctatctctt atttaaagtt gctatatacc ctcttatttt
      361 agcatacccc cttttataaa aagcaaaaaa aaaagaaaga aacttattct tgctaataaa
      421 ctacgcgaaa ggagtctaac gttgtttgat ataacttaga ctgatcgtta acttattttt
      481 tatatttcta catagttatc tttagaacca tttcagatac atatgaaaca attacatatg
      541 attttttcat aaaaaaacag tactgtctag tagtataaaa tatacaaatt tcttaaaaat
      601 tttttcacta aaatcactac ctgaaacagt ctaaaacact tatatatcaa cgtttaatga
      661 aactatctcc gtttcatttc agatacaaat aaatatagtc gggctccaaa tccgaaaaaa
      721 aataccccca gtagcttatc ctaagctact ggagatgtat aagtacggtc atataacaac
      781 ggggtagggt aaataaatga aaaagatgga agaatcatta ttcaataact tttttaaata
      841 cttcgttgta atcagttacc tgtccgttct taaataccac agaataatct tttttcccat
      901 tttcaagtaa ccgaagtgga atgtctttac caacttgctc tgtcactgca ttactgatac
      961 ctgttagatt agctaataaa tctgataaag attgagcatc tgtagaaatc tctgtatcag
     1021 tatttatcgt gattccttta tcttcattta caagcttcca agtaggtggt atagctgtgt
     1081 atgtttcgat gaatgaatca attctttcat ctatcgtctt cccagtatct acttctgtct
     1141 cattactctt agatgaatcg attctttcat ccatcgtctg ctccgtaact gcttctgtct
     1201 cattattctc accggaaact ctcttcacac ccaaacttat atattgtcca tctttagtgt
     1261 gcatagttaa aattcccacg cttttactaa taaattcact ataaccagtt ctttcaatag
     1321 agacttcaat gtatttcaca ctatcatact caaaagaaaa atagacatgt aaaagcattt
     1381 tgttgtttac atctacataa taatcctttg actctacttg agaccttcct atacggaata
     1441 cagctttact gtctgatatc tctataccag catccttatt attaatctgc caatctccaa
     1501 gtagctcttc tggcaagcta gtagtttctt gtgaacactt taccacttca ctggtagagg
     1561 tcgaaacatt cacctccgca ctgttagaag aattttccaa ggaatcagga gccttagcac
     1621 aaccaacaag tctttatata ttgtgcagcc gatattttct tcattcagta cacccttgaa
     1681 atatgcagtg ttagataaag cagttcgtcc tgatttgctg aggcacccat ctcttcttga
     1741 acgtactcct tgattttcaa ggccgcccga tactctttcg gaaaacgctg cttcactgaa
     1801 gccagcaaaa aatcatcctt ctccggcaac tcttccttct taatcattcg ggcaataaaa
     1861 aactgtaaat gcgtcacaaa tcttaagtaa agcatcgaat ctttatcaac ctttttttcg
     1921 ggaaacagat atcctagcat agccaagata tcctttagtg ctcttaccga gagcatggtt
     1981 tcttccatat ttgcttgttc actttgggca tttacaatgt gaaacgcaat gtttccagcc
     2041 tcctcttctg gcaactgaac attcattttt ttgttgatca attggatagc atagcagccg
     2101 atctcaaatt cttttgggta atacttttga atttcaatca gcaggcgatt ttgaaaaaca
     2161 atgccttttt gactgcgttc aatggcatgc ttcaagtgat caaccaaggt aaaaaataga
     2221 cttttattca gcttaaagtc aaacttggcg ttggcataat cgataattcg cttgacgcaa
     2281 ataattagat catccggagc agcctcaaac agtttaatgt aatccttttt tagagcatca
     2341 gaaagtgaat ctaatttaaa tactttttgg attttgtcag ccggaaccaa atcgcctttc
     2401 tttagttgaa atccaatccc tttccccatc aaaattactt ctttgccttg cttatctaag
     2461 caaagaatta aactgttgtt taacgtttta attactctca ttagattcac cgttctctct
     2521 aagctaagcc atttcttgcg attacttttt gataccaaaa gaagctttct ttttttattc
     2581 gttttaattc tttaagattc tcatcggttc ggtcaacata tataaaacca taacgcttat
     2641 tgaagccttg atgggtacta acaacatcaa tcgctgacca aggtaaataa ccaaaaacat
     2701 ctacaccatc tgttaaagct gcttgaatag ccagaatatg tttggctaga taatcaattc
     2761 gatcctggtc attaattcgt ctatcttcct caactatgtc gggtgcccca tagccatttt
     2821 cagtaattaa aataggtaaa tcatagcgat cgtacaattt cctcaatgtt aatcgtagtc
     2881 cagtagggtc aactacccag ccatatggcg ttttatctac ataaggattt tcagctgccc
     2941 ggtagacacc ttcttctccc aacatgatct gttgatcccc cgctctagcg gaaacatctg
     3001 atccgtcatt tttgctggca gcgatggtgg cagttgaata ataattaatc gcaataaaat
     3061 cgggtttacc ggaggataaa attgcctgat cctctgcctc aattttagga tataatccac
     3121 gatcattcaa atagttttca aacaaataat tgtattttcc ccgaactgct acatctaaga
     3181 aagaccagcc tctcaatgtt tcccaattat gggctgcaat tgcatcttct ggactggagg
     3241 tagcttgata catagccgtt aaattgattg ctggaccgat ttttccttta agattcaact
     3301 gatgatacaa gtgaattact ctagcttgag ctaataaaac atggtgatta atttgaaaga
     3361 gttctttttt tgaaggcagc tctccacttg gtggcaaacc aatcgctcca ggatgaagga
     3421 tcatcgtgtt ttgctcatta attgtcagcc aatagttcac cttgtcacca tactcagtaa
     3481 gcaatacctg tacatagtcc acaaaataat cgatagttgc tcgatttaac caaccgccct
     3541 gctcctctag aaaataaggt aaatcgaaat ggtacatggt gacaatcggt tcgattccct
     3601 gattcaggca ttcatcaatt acttgatgat aaaaagaaat tcccttttga ttggttttat
     3661 tattttcaat atccataatt cgcgtccaag cgatggaaaa gcgaaaagct tttaagccta
     3721 attctttaaa cagctgtata tcttctttat agtgatggta aaaatccgag gcaacttcaa
     3781 aattagctac cccttctgta tgctgatact tgtcaataat agacggttgt ttcccatcct
     3841 cctgataggc accctctgcc tggtaggcac taatggaggc accccataaa aacttctctg
     3901 gaaatgctga aagctgtttg tattgcacaa aaatcctctc cttaactaat ttgcatcaat
     3961 ggttcgtgtt cctttaagct tccgcttgct tcactaattc ctgcgtaatt ttcactgttg
     4021 gtcactacca caatcactgt atcatcgtag ccagcgtttc taatggcagc gaaatcggcg
     4081 ttgattaata attgtcctgc tgacacctga tctcctgctt ttacaaatac ttcaaagggt
     4141 tcccctttta aattcacggt gtcgatcccc acatggatca ataattcaat accttcttta
     4201 gaacgtagac caatcgcatg tttagattct aaaatagtaa tgacttctcc ctcaacaggt
     4261 gaataaatgc tgttttcttc tggatgaatg gctacgccct ttcccaatag tcccttagaa
     4321 aagacagcat cgttcacttc agaaatagga cacacggttc cttttaccgg attttcaaca
     4381 atgactgctt cggctattgc cgtttgctct gattcatcag aagtatcgtc aaatccaatt
     4441 agataagcaa tgataaaggt tgcaacaaag gcgattacac acgtaataat tgcgtgaaca
     4501 atattcattg gattttctcc aataaaggct ggtaaggctg ctaagccagg ggaaacaaag
     4561 gcatatctga ctaaaccgct caagccagca tacaaaccag caattgcacc accaatcatg
     4621 gaagcaacta atggtttttt cagcttcaag gttactccat agagtgaagg ctcagtaatt
     4681 cccattaaag cagtgatccc agtggacaca gctagctgct tcaactcttt gtttttagtt
     4741 ttcgcggcta cagcaaaggt agcacctgat tgagcaatat tagaagccag cattcctggc
     4801 ccattgatca tttcagaacc cgtactgctc aattgcatag tagcgattgg cgtcattgcc
     4861 caagctgtac ccgtaaccac caaaaatggc tgcaaaccac ccatcaaaaa tggaattaac
     4921 caagctgctt tgccgttaat ccattcagcc cccattgcta taagatcgtt cagcaacgtt
     4981 ccaaacggtc caatgacaac caacgctaaa ggccccataa ttaaaaatac cagcatcggc
     5041 ttagtaaaga atttaataaa cgaaggcgaa attcgatccg caaagggctc tacataggac
     5101 atgatccaaa cagtaaagat aatcggtaaa actgatcccg cataatctgc agctaatacc
     5161 ggaattccta gaaaagaaac ggcatcgcca gtagaaaaaa gctgtgaaat atttggatga
     5221 agcaaaatgc cagcaacagt aatcgccaaa attgggttga catcaaactt tatggctgcc
     5281 ccataggcta atagtactgg cataaaataa aaggctgcat cagcaatagt attcaaaatg
     5341 atgtaagttt gactactgtc tgatagtaca ccagtcaaag tcaagacggc caaaatggct
     5401 tttaccatcc cggcacctgt tatagctgga atcatcggtg taaaggtggt agaaatgaca
     5461 ctaatcaatc ttgaaataat actttcttga ttgtctgaag aggggccctt tttaattgaa
     5521 gttcccactt ccttttgaat ttccttgaag gctgtttgga cttcattgcc aatcaccact
     5581 tggtattgtc caccgctatc caccacacta attacaccaa gtgttttttc taaggctttc
     5641 gtatcggctt tccttttatc tttgagttca aaacgcagtc tggtggcaca atgggttaag
     5701 ctgaccacat tttcttttcc cccaacctgt tgcaatacat ttcttcctac ttctctgtaa
     5761 tccattattc tctctcctta aagcagtaaa aaagaccaag tcaaaccacg ccccaatcaa
     5821 cattgaagtg ttagtttgtc ttggtcttgc ctgcataatc agtaacatgc caataacgtt
     5881 attttgctgt aatccaataa tagcacatta tgatagcgca atcaacaggg aatttaattt
     5941 tttatctttt cgtttgaccg tatagggtaa tataacagtt agatcacatc aactagggta
     6001 tatctgcgta tacttactgc taaaataagt atat
//
LOCUS       DACEXQ010000166         6007 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.166, whole genome shotgun sequence.
ACCESSION   DACEXQ010000166 DACEXQ010000000
VERSION     DACEXQ010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.166"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..763
                     /locus_tag="IXH04_002151"
     CDS             <1..763
                     /locus_tag="IXH04_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ1852723.1"
                     /translation="HTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIEKSLQL
                     SHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMEDPKEVQ
                     DKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYEWAKRF
                     LDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNEPGYTR
                     YTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            756..1325
                     /gene="rnmV"
                     /locus_tag="IXH04_002152"
     CDS             756..1325
                     /gene="rnmV"
                     /locus_tag="IXH04_002152"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAQ1852724.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            1318..2202
                     /gene="rsmA"
                     /locus_tag="IXH04_002153"
     CDS             1318..2202
                     /gene="rsmA"
                     /locus_tag="IXH04_002153"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAQ1852725.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            2653..3282
                     /locus_tag="IXH04_002154"
     CDS             2653..3282
                     /locus_tag="IXH04_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852726.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(3406..4515)
                     /gene="ugpC"
                     /locus_tag="IXH04_002155"
     CDS             complement(3406..4515)
                     /gene="ugpC"
                     /locus_tag="IXH04_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAQ1852727.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            4724..5146
                     /gene="mgsA"
                     /locus_tag="IXH04_002156"
     CDS             4724..5146
                     /gene="mgsA"
                     /locus_tag="IXH04_002156"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAQ1852728.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            5469..6005
                     /locus_tag="IXH04_002157"
     CDS             5469..6005
                     /locus_tag="IXH04_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ1852729.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
BASE COUNT         1902 a         1085 c         1245 g         1775 t
ORIGIN      
        1 tcatacccat ttaaatgctg aacagttcaa tgaagatatt ccagaaacga ttcagcgagc
       61 acaagaaatg ggggtaacga aaatggcggt cgtgggcttc gatacgccaa cgattgaaaa
      121 gtctttgcaa ttaagccatg actatccaaa tatatacagt attatcggct ggcatccgac
      181 agaggctggc agttatacga aagaaatcga aaataaatta caagaacaat tgactttacc
      241 aaaggttgta gcattagggg aaattggctt ggattatcat tggatggaag acccgaaaga
      301 agtccaagat aaagtatttc gcagacagat tgctatcgca aaagaaatga acctaccaat
      361 cagtattcat actagagaag cacttgaaga cacgtatcaa atcctaaaag aagaagatgt
      421 tcgagatatc ggtggaatca tgcatagttt cagtggtgat tatgaatggg caaaacgatt
      481 tcttgactta gggatgcata tttcatttag cggtgtcgtc actttcaaaa aagcattaga
      541 cgtccaagag gctgcagcaa atgtaccaat cgaacgatta cttgtggaaa cagatgcccc
      601 ttatctggca ccagttcctt ttagaggaaa gcgaaatgaa ccaggctata ctcgttatac
      661 agtagaaaag attgctgaac ttcggaatct tccgacagaa gaagtggctg cccaaacatg
      721 gacaaatgcg caccgattat ttggtctaag cgaacatgga taagatgaag atccaagaga
      781 ttatagtcgt tgaaggaaaa gacgatacaa gaaggctgcg ggaagtagtt gatgcagata
      841 cgatagaaac aatagggtct gcaatcaatg aagagatcct gatgcaaatt gaacatgccc
      901 aagagactcg tggagtgatc gtttttaccg atcctgattt ttcaggagaa aaaataagaa
      961 aaatcattat ggaagctgtt cctcaggcaa aacatgcttt tttgccgcgc acacaagcta
     1021 aaccaaagaa aaagggaagc cttggtgtcg agcatgcaag tgatgctgct attttagaag
     1081 cattgaaaaa ggtcgtgacg ccagacattt cccaaacggc tgtttctgaa atcactagac
     1141 aagacctttt agactatggt ttaatagctg gggcacaagc aaaagaaaaa cgagaaaaac
     1201 taggtgacga gttgcgcatc ggttacacga acggcaaaca attggaaaaa cgtttgaaga
     1261 tgtttcgtat tacgccgaaa gaactcgccc aagcaatgaa aatagtggag gaacaacatg
     1321 agtgaatatc gtgaaattgc gacaccctct agaacaaaag agattttatt gaaacatggt
     1381 ttttcattta aaaaaagttt aggacaaaat tttctgactg aaccaaatat tttaaggaaa
     1441 atcgtagaaa ctgcgcaaat agatgaacat acgaacgtga tcgaagttgg accaggaatc
     1501 ggtgcattga cggaacaatt agccaagcat gcaaaacaag tcgtagcatt tgagatcgat
     1561 gatcgtttga tccctgtatt ggctgatacg atgcaaccct atgacaatgt gactattgta
     1621 catcaagata ttttaaagac cgatttgtca acagctgtta gagaaacatt tcatgaggag
     1681 cttccgttaa aagtcgtagc gaatttacct tactatatta cgacaccgat catgatgcat
     1741 tttcttgaat cagatttggt tgtggatgaa ctagtcgtca tgatgcaaaa agaagtagct
     1801 gatcgtatct ctgctgaacc aggaacgaaa gcttatggtt cattatcgat tgctgttcaa
     1861 tattatatgg aagccagttt agcattcatc gtaccaaaaa cagtatttgt ccctcagcca
     1921 aatgtagatt cagcgatttt gaaactgaca agaagagata taccggcagt cgaagtgaca
     1981 gacgaaaaag catttttccg attgacaaaa gcggcatttc agcaaagaag aaaaacgctt
     2041 tggaataact tgcagcattc gtatggcaaa gatgaccaga caaaagcatg gttagcaaaa
     2101 agcttggaaa cagctggaat cgatccaaaa cgtcgtgggg agactttgtc attacaagaa
     2161 ttcgccgcat taagcaatgc gatgtcagaa aatatgcagt aaaaaaagag gttgtgacag
     2221 aagttgtgaa aaagctgtcc tgcatcaagt aataagaaca gccaaacaaa aatagttcct
     2281 catgtttttc gtttgtttgg gcttattacc gcagatgcaa gcttttgaac accgtttaag
     2341 aaagaggttg tgggcctgcc gtttaactct aagtcacaag gaacaatttc aaaaaacagc
     2401 ttctcatgtt tttgagttag ttggtcttat tacttgatgc aagcttttga ccactattat
     2461 gccgaacaag gagatgtgac acaaattttg tcacatctcc tatttttgtt tcttgatcaa
     2521 aaaattctta tacttgatac tcacactcat tttgtatgga tttagaaaag aatatgtgtc
     2581 ttgacagtta ttagtaagtg agttatacta agtcgacatt tatgtgtcaa gacatattag
     2641 gagagagaaa aaatgagaaa aaattcagtt cgaaaactaa cgatttctgc tttattgatt
     2701 gggatggggg tcattatccc aatggtcatg cctaaaatca tgattccacc agcttccttt
     2761 actttggcgt ctcatgtgcc attatttatt gcgatgtttt ttacaccagg agttgcagtg
     2821 gctgttgcgc taggtacgac atttggtttc tttttaacaa cgccggttat catcgcattg
     2881 cgagcattgt cacatcttgt gtttgcgttg attggggctt ggtacttgca aaagcatccg
     2941 agtatcgttt taaaaaatgg acagtttact tttgctaatt ggcgtttcca agggttcaat
     3001 tttgtgatcg gtgtgattca ttctttagca gaaatgctag tagtcagcat cttttacttt
     3061 attggtaata tgcctgaaac atattattct caaggatatt tttatacagt attcatcttg
     3121 atggggattg gtgggcttat ccacagttta gttgattata atattgctta ttttatagca
     3181 ggaacactca gcaaatattt tgatatcccc gtatttacgg cagcaaaaaa agaaaatcgg
     3241 aaagagtcga ttcaagggta cgcgaagaaa acgacatatt agacatgatc tctcaaaaaa
     3301 aaaactctca tctagtaatg gataataaat cctctaagat gagagttttt tgtcctaaaa
     3361 agagctgcac caacatcttc tggcacagct ctaacagggg gcaatttatg gaagtttgat
     3421 gacttgttct gtttctttat caaagaagtg gcctttgctg atgttgaagg caaggtcgac
     3481 tatttcgcct ggtttatgga aatctcttgc atctactttt gagatgaact cagtagaacc
     3541 agtttttgtg taaagcatcg tttcagctcc aagtaattcg gaaacgacaa cttctgattt
     3601 cactacggct tcaggtgaag cttcaagggc tacttgttca ctgtggatgt cttctggacg
     3661 aataccgaag ataacttctt ttccttcata acctttttca actaatactt tgttttttcc
     3721 ttcaggaata cgaagcttaa gcccatgtcc atctgtgata acaccatttt tcaatgtcac
     3781 attaaagaag ttcatggcag gagatccaat aaagccggca acgaacatat tgacgggtgt
     3841 atcataaact tctttcggtg taccgatttg ttggataaat ccgtctttca tgataacgat
     3901 tcggtcagcc atagtcattg cttctgtctg atcgtgagta acatagatgg tcgttgtttc
     3961 aagacgctgg tgaagtttgg caatctcagc acgcatggct acacgtaatt tagcatctaa
     4021 atttgataat ggctcatcca ttaagaagac ttttgcatcc cgaacgattg cccgtcccaa
     4081 tgccacacgt tggcgttgtc caccagataa agcagctggt tttcgtttca agtattctgt
     4141 cagacctaag atctctgctg cattgtccac acgttttttg atgtctgctt tatcatattt
     4201 acgtaatttc aagccaaaag ccatattatc aaaaacagtc atatgaggat acaatgcgta
     4261 gttttggaaa accatggcaa tatcacggtc tttaggtgcc acgtcattca tgacttttcc
     4321 gccgatattt agttctcctt cagagatatc ttccagtccc gcgatcattc gcaaagtagt
     4381 tgactttcca caaccggaag gaccaacaaa aacgataaat tcacggtctt tgattgatag
     4441 attaaaatca gttacagaat aattttctgc attgtcatat tttttatata cgtttttcag
     4501 agccatttct accataatac gttccacact ttctttttta gttttattaa ctatttaaag
     4561 tataaatgaa agcgttaact aacttcaatg tcatgttgaa caagaatttt ttcttttttt
     4621 tgtcaatgtg ctagttttaa gctttaataa atatccataa gaaataaaaa ctgatacact
     4681 ggagtaaata agtatgttaa caatatcaaa aggagtgtct cttatgaaaa tcgctttgat
     4741 cgctcatgac cgaaaaaaac ctatgatggt gaaattagca acagcgtata aaacaatatt
     4801 ggaaaaacat gaactttttg ctaccgggac gactgggcag aggatcacgg aagcaaccgg
     4861 cttgccgatc catcggttta aatccggacc gttaggcggg gatcagcaaa ttggagccat
     4921 gatttcggag gatcagttag atttggtgat ttttcttcga gacccgctgg ctgcacagcc
     4981 ccatgaacca gatgtaacag cacttatccg tttatctgat gtctatgaga ttccgttagc
     5041 tacgaatatc ggaacagctg aggtgctact aagaggactg gaagccggct tcctagattt
     5101 tcgaagaatc gtccatgaaa tagacaagca tccactttca ttttgatgga taagcgtatg
     5161 gaaaaataac gtttaatgtt cgcctatatg tagctataaa agctaaacaa actgataata
     5221 tgcgatataa tacgaacgta ttggataata taaacaataa tattagtttt tcgtatttgc
     5281 agtaaatgtg aaaatatgct atagtttctg tgggaaaaga tcccaaaaat aatacagagt
     5341 agaaaataaa gcgttaagtg ctaagaggat gggatgttgc ctcttggacg aaggacgtaa
     5401 tgttcgcggt tttattttcg cattcgctgc atgatacgat gtagcagctc ttaaaggaga
     5461 tgctaaaaat gaagaaacac cggttagacg cacgtatgat tgctattatg ggcttattga
     5521 ttgcattgat ggtcactttg tcccgactgg tagcaatcga gaccccattt attaaaatca
     5581 gtgtcacatt tattccacaa gtgatcatgg gcattctttt tggtcctttt tggtcaggga
     5641 taggcgcagt tttagctgat ttagtaggaa tggccctctt ctcgaaatct gcattcttta
     5701 ttggatttac attgaatgca tttattgaag gagcaatcta cggattcttt ttctatcgaa
     5761 aagagatcac ttggaaaaat gccattctgg ctacgcttag tgtcacgctg atcatcaact
     5821 tatttttgac accattatgg cttgcgttga tgtaccatgt gccattgttc agctgggtcg
     5881 tttgggcgcc ccgcctgtta aaaacagtaa tatggttacc gattcaatca attgccattt
     5941 attacgtagg acgtagtatt ccttacaaaa aaattttaag aagtttagcg atccacgcta
     6001 aataaaa
//
LOCUS       DACEXQ010000167         6003 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.167, whole genome shotgun sequence.
ACCESSION   DACEXQ010000167 DACEXQ010000000
VERSION     DACEXQ010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.167"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            30..233
                     /locus_tag="IXH04_002158"
     CDS             30..233
                     /locus_tag="IXH04_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1852730.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            249..587
                     /locus_tag="IXH04_002159"
     CDS             249..587
                     /locus_tag="IXH04_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HAQ1852731.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            574..753
                     /locus_tag="IXH04_002160"
     CDS             574..753
                     /locus_tag="IXH04_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852732.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(796..1266)
                     /locus_tag="IXH04_002161"
     CDS             complement(796..1266)
                     /locus_tag="IXH04_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852733.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            1353..2051
                     /locus_tag="IXH04_002162"
     CDS             1353..2051
                     /locus_tag="IXH04_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAQ1852734.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            2229..2570
                     /locus_tag="IXH04_002163"
     CDS             2229..2570
                     /locus_tag="IXH04_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852735.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            2563..3234
                     /locus_tag="IXH04_002164"
     CDS             2563..3234
                     /locus_tag="IXH04_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAQ1852736.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            3240..3926
                     /locus_tag="IXH04_002165"
     CDS             3240..3926
                     /locus_tag="IXH04_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852737.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            3929..4678
                     /locus_tag="IXH04_002166"
     CDS             3929..4678
                     /locus_tag="IXH04_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1852738.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            4690..4959
                     /locus_tag="IXH04_002167"
     CDS             4690..4959
                     /locus_tag="IXH04_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852739.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            5121..5423
                     /locus_tag="IXH04_002168"
     CDS             5121..5423
                     /locus_tag="IXH04_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852740.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            5420..5581
                     /locus_tag="IXH04_002169"
     CDS             5420..5581
                     /locus_tag="IXH04_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ1852741.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            5578..5883
                     /locus_tag="IXH04_002170"
     CDS             5578..5883
                     /locus_tag="IXH04_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852742.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            5883..>6003
                     /locus_tag="IXH04_002171"
     CDS             5883..>6003
                     /locus_tag="IXH04_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAQ1852743.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETG"
BASE COUNT         2256 a          916 c         1275 g         1556 t
ORIGIN      
        1 gaggaaattg gttagaaagg agttttagta tgactgacat tgcagaaatc actcaacgag
       61 atagagaaaa aatcaaagaa tatgtcgaaa gttcaaagtt cttaacttac accatgcttg
      121 ctgaaagatt tggaattagc aaaagctact tatctttaat tttaaacggt aaaaagactt
      181 ctgcagaagc aaacagaatt atagattcga ttatcactat gtacgaattg taaagggagg
      241 aacagctaat gcaatatcta gaagcaaaaa tcccaattcc agaaggctat gtaattatct
      301 cccaagtgga ttatgaggag ttaaaaaaag ctgatgatac tggtagatgg atgacgttgc
      361 cagaagtgct agaacggatt aacagaaaat atgattggtt tacttctaga gttttaaaga
      421 acccaagata tagaaatatt atcgatatcg agaaaaacaa aaatggattt gtctattatc
      481 cagttgaagg aagagacaca tatctatttt taagaagtaa aacacttgaa tttttagaaa
      541 caaatttttc ggaaatctta aggaggcaag cggatgggaa aatttaatag agcgctagtg
      601 ttcagcgcac cgctaatcat ctacgcttta ggactttggg gaagcaggca agcgttgata
      661 ggaacgattg tttacatggt ttggattttt atggggcttg atgaagctga agctgagtac
      721 agagcgaaaa agccaaccga gggggctgac taaaaatgaa tagaaaagag aaactagaat
      781 gtatattact attactcagt ttaattcttt cactaatttc tctattggga agtttgtatt
      841 tttgatatca acaattttgt ggatttgggt tctatttaga gtaaatatca tttttgtttg
      901 gtgattaagg agtttattaa aattaatttt ccctacttca aatgcaaggt aacaagatat
      961 agcactttta ggaggaatat ttagcggtac agcttcagag tatattctat tatcatcgac
     1021 tgatcctcct ttagcaatta atttcttaaa tggagtagct gaccatctgc ggtttaagtt
     1081 agcggatgaa agttctaaat taactaacat agctggttct gttgaatagt tagaaatgat
     1141 tacttttgta taaatcatat ctgcagcgaa gtaagaagca ttaagttcaa cttctagttg
     1201 agggcgcatt ccttttattt ttatccctgt taaaaacgta ctcaatataa aacttattat
     1261 tgacatccat tgaaaaattg ttaagtgaaa aaaattcaaa ataaacacca ccagttttta
     1321 actaaattat accaaaaagg agagaagaaa taatgcaaga attagtaatt ttgaaaaata
     1381 aagaagctgt gactacgagc ttacaagtgg cagaaagctt cgagaaaaaa catcaacatg
     1441 ttttaagaga tattgatgca ctaaaaaaag atgtgtccaa ttttggacag atgtttgtag
     1501 aaggtaatga accagattca tatggcagaa atcgacgagt tttcttcatt agtagagatg
     1561 gatttttctt gctggctatg gggtttacag gaaagaaagc tatctacttc aaacaaaaat
     1621 acattgaagc attcaacgaa atggaagatg ttattcgcaa gaatactgtt cctcaaacaa
     1681 ttgaagatat gatgatctat caattagaag aaatgaaaga tgttaaaaaa gatgtttcca
     1741 tgcttaaaga tactatgcga attagcggac aacaagagtt tgaaattaag caaaaaggaa
     1801 atatgaaagt tatgggagtt ctagggggaa aagaaagccg agcttatgaa gaaatcagca
     1861 aaaaagtatt ctcaaaattt tggtctgaat ttaaacgtac cttttcaatc ccaagatatg
     1921 gcgagttacc tcgtaagaga ttcgatgatg ctgtttcatt tattgaaatg tggttgccag
     1981 aaactgcgat ccgtatggaa attgatcaac tgaacagaca acagagactt tttggtgatg
     2041 acaatgaata gagctgaagc gctaagaata gggacggcaa ttgctaattg ctggtggaaa
     2101 tactataaac caatcatcct aagccaacaa catattgaca agcaaaaagc atggcaacaa
     2161 ataaaaaagt gactccgccg gcaagcaaag agtcacaaag aaaacacatc ataaggagat
     2221 tttagcatat ggaaaaagaa ctttccactc tagatcaata tttgattgat cctgattggg
     2281 gcaagccgaa aattgaggaa acaagtggtc gaaaaatcag acgaaatctt ttgacgaatg
     2341 aagaactagc ttgggatcaa gatgatttag gcaaccatgt aactatttgg gatcatgttt
     2401 atcttatcca tctatcgaag cattcgaata aacctgaata tatttacgtc atcgaagatg
     2461 gcttgattga tgcgctagaa gagtacgaca gagataactt gattgatatc tcttattacg
     2521 gaccaggtaa gaaatacatt gctgaaatgg aggcagaatt tgatgagtga aatcaaaggg
     2581 acaacgaact ttgaaaaact ttttagtcgt aagttaaata aaattctcaa gaaaaaagga
     2641 aattttgatt atttatcttg ggctcacgcg tgggagatta tgaaaaagaa tgatccacag
     2701 gcaacggtaa ctattaatga gtacaaacac tacagggttg tttctggaac tcatcaagac
     2761 tttcttgttg aggaatataa accttttctt atggatgaaa ctgggactta tgtatctgtc
     2821 tcagtaacgg ttaaaggaca cacggaaacc gaattatttc ctgttttaga ttatcgaaac
     2881 caaccagttg ttaaaccaaa tgcaatgcaa atcaataact cattgaagcg atgctttgtg
     2941 aaagcattgg ctctacacgg actgggatta tatgtatttc aaggggaaga tattccaaca
     3001 ccacctagaa tcgatacaaa gaaattaaac atgctagaga cgattctaga agctttcaat
     3061 gagcagatgg gtaaagatat gaccaaaacc ttaatcgaat atgttaatga gcagacagat
     3121 aaattagggc tcttagctga taacgttgaa actattgaac agttaagcta tgagcaatgt
     3181 gccttgatgg agcgagcaat agcagctaag agaaaagaat tagataagaa gtgatatgag
     3241 tgtttaaacc attaatcgat tcatattcag cggttctgaa aaagttcaaa ggaaaagaca
     3301 taagcgcaac catcaatgag gaagtgaaca ttgatcgact aaagacgatg tatgacggct
     3361 acgatggtga tcgagtcatt gaaattcgtt ttattgatcc tagacgtttc accgtacagc
     3421 aacgaaactt catctatgcg ctgataggcg atatttttat cgatacaggc atgccaacgg
     3481 acttctggaa ggaattcttc tactttcgtt ttgaaggtgt cacagggcgc aaaataagcc
     3541 tgaaagacga atcgaataca actgtgagtg atgccaatgt cttagcaaat atcatcttag
     3601 atttcatctt tgaacatcat attcctttca aagaaggcta tgagatttta cctgcgaatc
     3661 aagaatatta cttctacaaa tgcatcacaa aaagagtctg ctgcatctgt ggcaaaacag
     3721 gagctgacat cgatcacttt gacaaagcgc taggaagacg aaagcgcaaa gaagttgatc
     3781 atgcagagta cacatttgca gcactctgca gaatccatca cacagagaag cacaaaatag
     3841 gtgtgattaa tttcaaaaat aaatatcaaa tcaaagggat caaattaaac cagaaaacaa
     3901 tcaaaaagtt aaatattgga gggtaaaaat gacagaacat cgaagttatt atgcgattat
     3961 accagccaac gtaaggtacg acaaaagact taaaccaaat actaagttgt tatacggaga
     4021 gataacggcc ttgtgtaatg aaagaggctt ttgttgggca ggcaatgagt actttgcaga
     4081 tttatatggt gtgaataaag agaccatatc gcgatgggta agtgatttga ttaagtttgg
     4141 atacttgaat cgggaaatca tttacaaaga gggtaccaat caaataatca ataggtacct
     4201 acgaattaat caatacccta ttgacgaaaa acgcaatacc cctattgacg aaaaagtcaa
     4261 agataataat acatctatta ataatacatt taataataca aaagaatata taagagagtt
     4321 accgccttcg aaaaaatcga aggctaagcc cgtccgtcat aaatacggag agtataaaaa
     4381 tgttcttttg tcagatgagc agatggagaa actcaaaaca gaattcccta atgattacca
     4441 agagcgaatc gaacgactgt cagagtattg tgaatcatct ggtaagactt ataaaaacta
     4501 tttggcaact attcgaagtt gggcaaggaa agaaaaaagt gaacctaaga acgcaagcag
     4561 tggatacaag cgcacaggaa gacgagagaa gcttcctgaa tgggcaatcg accaagaagc
     4621 ctatctcaag aaaaaagcgc tagaacgagc taatagacaa tcaaaagcac cattctaaga
     4681 ggtggaaaaa tgaagatcga ttatctagaa ctaattaatg aaatagcgaa ttataaaaag
     4741 ggcgaggaat tagacgtcct gagagacgta tatgatcaac tcgaagaagc tggaattgaa
     4801 ggaattaaga atgatcgttc gagttggagt aagctcagat actatttcgc actttatatc
     4861 gatacaacac aattaagaaa tttagcatat accaaattac tatttgttga ttgtgttaaa
     4921 ggcttgcaaa aacatcttaa tgaacttgag caggtgtaat cagatggacc taaagacatt
     4981 tacagcacag attgaactaa tgcatcaaga agctttaaga caaagtgtat cgtacgaaga
     5041 caagtggctc aacacgttcc atggcggacg tgagagcgca cttgatcaag tactcaaatt
     5101 actgaaagga gaatgtcggg atggataaga aagcggcaat gcagcgaatt atcgaattga
     5161 cttattcaga agattggcaa aatgacaaag aagctgcttc agaagtgatg aggcttggaa
     5221 gagcgatgtg ggcagacaag agcaacaagc caagaccacg aaaaatcgca atttggcacg
     5281 gtgacaaact tctagtgata gggacagctg aacagttagc aagtctcaca ggcttgcacg
     5341 agaaaatcgt gaggaaaaga gcaaggtgtg gctacacaga cgctaagaag agaacgttta
     5401 gatacgtgga ggaatcatca tgacaacaga agaagtgatt caaatgcgta ttcgaagcct
     5461 tcagcgtgag attgacgatc tggaacgaac aaaggcagtg atggtcaatg aaacggcgag
     5521 aaaggcaatc gatttgcaca tagagaattt aagaagggaa atccatcgat tggaggaatg
     5581 agcgtggata agaaagcagc aatgaaaaga attgctgaat taaccaagtc agaatcttgg
     5641 caagaagaca aagaaatagt tgcagaagtc caaaagctcg gtaaatcaat gtggactgaa
     5701 aagcccaaac ggagaacgcc gagaaaaatt gcaatctggc atgatgaccg aattttagta
     5761 acaggtactg ctgaacagtt atctgaaatt actggattaa gcaaaaacat tatctgggat
     5821 agagctagga gcttatggat tgattcaaaa ggacgacagt ttaggtatgt ggaggagaga
     5881 taatggatct cattacacaa tacagtgaca tcatcctcaa gaaaatcatg atgaagattc
     5941 agaaagataa aaaatcaaaa gaacgagctg aattagttaa gttagaaatg gctgaaacag
     6001 gag
//
LOCUS       DACEXQ010000168         5990 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.168, whole genome shotgun sequence.
ACCESSION   DACEXQ010000168 DACEXQ010000000
VERSION     DACEXQ010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5990
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.168"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..512
                     /locus_tag="IXH04_002172"
     CDS             <1..512
                     /locus_tag="IXH04_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase II"
                     /protein_id="HAQ1852744.1"
                     /translation="TTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDL
                     SIEKRNQELVLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFA
                     ETQSRFVLTVAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCW
                     EDAIVCLMK"
     gene            497..1936
                     /locus_tag="IXH04_002173"
     CDS             497..1936
                     /locus_tag="IXH04_002173"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAQ1852745.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            1968..3011
                     /locus_tag="IXH04_002174"
     CDS             1968..3011
                     /locus_tag="IXH04_002174"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAQ1852746.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            3008..3586
                     /gene="purN"
                     /locus_tag="IXH04_002175"
     CDS             3008..3586
                     /gene="purN"
                     /locus_tag="IXH04_002175"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAQ1852747.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            3583..5118
                     /gene="purH"
                     /locus_tag="IXH04_002176"
     CDS             3583..5118
                     /gene="purH"
                     /locus_tag="IXH04_002176"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAQ1852748.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALVFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            5134..>5990
                     /gene="purD"
                     /locus_tag="IXH04_002177"
     CDS             5134..>5990
                     /gene="purD"
                     /locus_tag="IXH04_002177"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAQ1852749.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEF"
BASE COUNT         1949 a         1017 c         1464 g         1560 t
ORIGIN      
        1 tcactactca ggaattcaaa aaatcagggg atctaatcta tatattaggt aagacgtttg
       61 cagattttga tggcagtgaa ttacaaaaga tgcagcttgg aagaatcgaa ggtgtgatcc
      121 gaaactttga tctatccatt gaaaagagaa accaagagtt agtattgaca gccatccaaa
      181 acggattgat tgaaagtgct catgactgtt cagaaggtgg cttggcaatt gcactggcag
      241 aatcagcttt caaacatcag ctagggatat ctgtacagtt tgaattgtcc tctgctcaat
      301 tatttgcaga aactcaatca agatttgtgt tgacagtcgc accggaaaat aagacacgat
      361 tcgaagaaat gatgggagat gctgctgttt tagcaggtaa ggtgacggat gaagcgataa
      421 tcgaaatttc agcaactgac ggtcagatca aaatagaaac tgcagtagca agaaagtgct
      481 gggaggatgc aatcgtatgt cttatgaagt gaagagttta aatgaagaat gcgggatctt
      541 cggtgtttgg ggtcatccag acgccgctag tgtggcatac ttcgggctcc atagtcttca
      601 gcatcgagga caagaaggtg caggtatcgt gtccaatgat cgaggaaagc ttaaggggca
      661 tcgagatttg gggctgcttt ctgaggtatt caaagatcaa agaaaattgc agcagttaac
      721 aggagaagct gcaatcggac atgtacgtta tgcaacagca ggcaatggaa gtgtggacaa
      781 tattcagcct tttctattca aatttcatga tcagtcgatc ggcttagcgc ataatggtaa
      841 cttgatcaat gctcgttccc ttagaaaaga gttagaggaa aatggggcga tcttccattc
      901 taactccgat acggagatat tgatgcattt gatccgacga agtgaagaaa cagatttttt
      961 aaataaatta aaaagtgctt tgaatgaagt caaaggtggc tttgcctatt tattaatgac
     1021 agaaacggca atgattgccg ctttggaccc aaatggtttt cgtccattag caatcggaca
     1081 aatgaaaaac ggagcatacg tgattgcctc ggaaacttgt gcattagaag ttattggggc
     1141 acgttttata agagatgtcc agcctggtga gatagtgatc atcaatgacg aaggaatcca
     1201 gattgattgc tttacgaagg acacgcagct ttctatttgt tcgatggaat atatctattt
     1261 tgccagacca gattcaaaca tcgcagggat caatgtgcat actgcacgga aaaatatggg
     1321 acggaattta gcgaaagaat cgccagtaaa agcagatatg gtcgttggcg tacctaattc
     1381 ttctttgtca gccgcaagcg gttatgcgga agctagtgga atcccttatg aaatcggatt
     1441 agtaaaaaat caatacattg cccgaacatt tatccagcca acgcctgagc taagagagca
     1501 aggtgttcga atgaaattat ctgctgtacg aggagtagtg gaaggtaaaa aggtgattat
     1561 ggtcgatgat tcaattgttc ggggtacgac aagcagacgg atcgtccatc ttttgaaaga
     1621 agcaggggca aaagaagtcc atgtgaggat tgcttctcct cctttgaaat atccatgttt
     1681 ttacgggatt gatattcaaa cgcgtaaaga actcattgcg gcaaatcaca cgattgatga
     1741 aatcaaagaa tgtatcggtg cagattcttt aggtttttta agtgaagagg gtctgatcga
     1801 atcgatcggt ctggaaaaag atgctccgta ttcaggattg tgtatggctt atttcaacgg
     1861 agattatccg acgccgttat atgactatga agaaaaatat caggaatcac taaaagaaaa
     1921 agtatcgttc ttttaaaagc agaccaagaa gggagtatat gaattcaatg gcaaatgcat
     1981 acgcaaaagc tggcgtagat gtcgaagctg gatatgaagt agtagaacgg atcaagaagc
     2041 atgtcaaaaa aacggaacgt ttaggtatga tgggcgctat aggaggattc ggcggttgtt
     2101 ttgatttatc ggtctttcaa ttaaaagagc cagtccttgt ctcggggacc gatggcgtgg
     2161 ggacgaaatt agcagtggca atcgaagcag acaaacacga tacaatcggg atcgactgcg
     2221 tagcgatgtg tgtcaatgat atcttagcac aaggcgcaga gccgttatat ttcttagact
     2281 acttagcaac ggggaaaaat gatccgttac gtttggaaaa tgtagtaaaa ggtgtagcag
     2341 atggatgtgt tcaaggaaat atggcgctga ttggcggaga aacagctgaa atgcctggta
     2401 tgtactcagg taatgattat gatctagccg gatttgctgt agggatagct gaaaaatctc
     2461 aattattgac aggagaaacg atcaacccag gagatgtgtt gcttggaata gcatctagcg
     2521 ggattcattc aaatgggtac tctcttgttc gcaaaatttt ctttgagcag cagcagtttt
     2581 ctttatcatc aaaactgcca gaattgaaaa aaacacttgg cgaagaattg cttgagccaa
     2641 cgaaaatcta tgtttcttcc ttgcttccgc tgatcaaaga aaagaaaata aaaggagctg
     2701 cacatatcac tggtggaggg tttgtagaaa atatcccgcg aatgttacca gaatctatgg
     2761 ctgcacaaat caaacttggc tcatggccga ttcttccaat attcggctgt ttacaaaaat
     2821 atggtgaaat tcctgagatg gagatgtatg aaatcttcaa tatgggcatc ggtatggtcc
     2881 tggcagtcga agaatcgaaa gtaaaagaaa tagtacacga aattgagcaa gctggcgaaa
     2941 aagtttatga aatcgggaaa gtgatcgcca aagaaaaaca tcgtgtggtg tttataggag
     3001 aagaacgatg agaatcgctg tttttgcttc tggaaacgga agcaactttc aagcgctagc
     3061 cgattatcta tcgaaaaaag gattggaatc ttcgattgac tggttgtttt gtgatcagcc
     3121 tgaagcatat gttttgaaac gtgcaactgc gttgagtgta ccggcagatt gtttttcacc
     3181 taaagaattt gattcaaaaa aagagtatga agaagcaatt ttgcataaat tgaaagaaaa
     3241 aaagatcgat ttgatcgtat tagctggata tatgcgaatc attggaccgg tattgctgaa
     3301 aaattatgat aaaagaatca tcaatatcca tccgtctctt ttacctgcct tccctggttt
     3361 gcatggcata cgtgatgcct ttgaagctgg agtaaaagaa acgggtgtca caattcatta
     3421 tatcgaccaa ggtgtagata ctggaccaat catccggcag gaaaaagtga gaattgaaca
     3481 ggaggacacg ttcgattctt tagaagaaaa aatccataga gtagagcatc gaatctatcc
     3541 agaagtgatt agtgaaatta ttgaaaatgg aggctatctg aaatgacaag agcattgatc
     3601 agtgtatcag ataaaactgg gattgtagca ttcgcccaag gtttgattga acaaggagta
     3661 gaaatcattt caacaggagg aacaaagcat gcgttggata aagctgggat caaaacgctt
     3721 tcgatcgaag aagtgacagg gtttcctgaa atgatggatg gccgtgtcaa aacacttcat
     3781 cctaaaatcc atggtggtct tttaggaaga cgcgatctcc ctagccatat ggaggcaatg
     3841 gaaacggaaa atatccaacc aatcgacttt gtttgtgtca atttatatcc tttcaaagaa
     3901 acgattatga aaccagatgt cgaaatagca gaagctattg aaaacattga tatcggtggt
     3961 cctagtatgc tccgtagtgc agcaaaaaac catgaatttg tcacctctgt tgttgatcca
     4021 tctgactatg aagaagtact ttcagaatta cgtcagtatg ggcatacttc actttctttc
     4081 agacaaaaac tagcggcaaa agtctttcgt catactgcgg catatgatgc actgatcgct
     4141 caatacttaa cggattgggt ggaagaaaaa gaaccggaaa aattgacact tacgtatgaa
     4201 cgaaaacaaa cgcttcgtta cggtgaaaat agccatcaag ccgctgcctt ttatcagtcc
     4261 gctcttctgg aaaaatattc gatcgcagca gcaaaacaac tccatgggaa agaactttct
     4321 tataataata tccgagatgc agatgctgcg ctacggatca tgaaggaata tgacgagcca
     4381 acggtagtcg ccttgaaaca catgaatcct tgtggtattg ggacagcatc tacgatttta
     4441 gaagcgtggg aagcagcgta tgaagcagat cctgtttcaa tctttggtgg gatcatcgtt
     4501 ttgaatcgag aagtagatct gccaacagca gaacagatgc ataagctgtt tctagaaatc
     4561 atcattgccc cttcttatac acaagaagct ttggaattac tgcaaaaaaa gaaaaacatc
     4621 cgattactga cagtagactt tgcgcaggaa gaaaatcaaa atcgagaaga aacggtctca
     4681 gtcttaggtg gacttcttgt acaagatcaa gatctggcaa tagaaaatga agaagagtgg
     4741 aaagtcgtga caaaacgtaa gccgactcca gcagaagcaa aagcattagt gtttgcttgg
     4801 agagctgtca aacatgtgaa atctaatgcg atcgtactgg caaacgaacg tcagacagta
     4861 ggaatcggag ccgggcagat gaatcgagtc ggttcagtaa aaattgcgat tgaacaagcg
     4921 caagagaaaa tggaaggtgc agttttagct agcgatgctt attttccaat ggatgattcg
     4981 gttgaatatg cagccaaaca tggtataaaa gcaatcattc agccaggagg aagcatcaaa
     5041 gaccaagatt ctattgatat ggcgaataaa tacgatctag caatggtctt tactgatgtt
     5101 cggcatttta gacattagag aggagtcgga taaatgaaca ttttggtgat cggtgcaggt
     5161 ggtcgtgaac atgcaataag taaaaaactt ttagccagtc cgaaggtgga tacggtgtat
     5221 tgtgcgccag gaaatccggg gatgacaaaa gatggtatca attgtgtcgc tatcagtgag
     5281 cgagatcatt cagcgttaat ccattttgtt aagaaagagg ctattgattg gagtatcgtt
     5341 ggtcctgaag ttcctttgtt aaatgggatc gtcgatgatt ttatggcgga aggattgaaa
     5401 atcttcggtc cgacaaaagc agcagctctg atcgaaggtt cgaaaagttt tgctaaacaa
     5461 ttaatggata cttatcaaat cccgacagct aaatccagaa gttttgaatg ttacgaaaaa
     5521 gcagcagcgt atatcaaaga aaatggtgcg ccaatcgtag tgaaagcgga cggcttagca
     5581 gctgggaaag gtgtagtcgt ggctaccaca gtgaaggaag cgctggatgc ggcagaacaa
     5641 atgttgaacc agcatcgatt tggggatagt agcaaaaaaa tcgtcgtaga agagtttttg
     5701 gcaggagaag aattctccct tttggcgttt gtccgtaata ccaaggtgta tccgatggtc
     5761 atttcgcaag atcataagcg ggcatttgat aacgataaag ggccaaatac aggcggaatg
     5821 ggtgcttatt caccagtacc gcaaatttca aaaaagatga ttgaaaaagc tgtggaagaa
     5881 gttttaaaac cagcggcttt gggaatgaaa gaactaggac gctctttcac gggtgtttta
     5941 tatgcgggtt tgattgctac gaaggaagga ccaaaagtga tcgaattcaa
//
LOCUS       DACEXQ010000169         5886 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.169, whole genome shotgun sequence.
ACCESSION   DACEXQ010000169 DACEXQ010000000
VERSION     DACEXQ010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.169"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(43..681)
                     /gene="plsY"
                     /locus_tag="IXH04_002178"
     CDS             complement(43..681)
                     /gene="plsY"
                     /locus_tag="IXH04_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAQ1852750.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            808..1242
                     /locus_tag="IXH04_002179"
     CDS             808..1242
                     /locus_tag="IXH04_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAQ1852751.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            1408..3453
                     /gene="parE"
                     /locus_tag="IXH04_002180"
     CDS             1408..3453
                     /gene="parE"
                     /locus_tag="IXH04_002180"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAQ1852752.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            3465..>5886
                     /gene="parC"
                     /locus_tag="IXH04_002181"
     CDS             3465..>5886
                     /gene="parC"
                     /locus_tag="IXH04_002181"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAQ1852753.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDRSIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVI"
BASE COUNT         2099 a         1014 c         1318 g         1455 t
ORIGIN      
        1 atttgtcaca ctcccgtttt atctctttgg aatgaaaaag tatcaggatt ttttcttgct
       61 gctattcaaa ccaaacggga ttcgactttc agtatggttt ctgatacgct tgatattgtc
      121 tttatgccga taaaagataa aggaagaaac taaaaaggca atgatcgtca gtaaccaatt
      181 gggcttagcc aaaatcacag gagctacata aggaaggacg atcgtagata aagtgatcaa
      241 tactgcactg atcatactgc tcaaactcac catacttgta caaaataaac taacgataaa
      301 gatgatacaa gaataaacaa aaaatacagg attaaatcct aaaagcattc ctgcacttgt
      361 cgcaacagct tttcctcctt tgaacccagc aaaaatcgga aatgtatggc caagaatggc
      421 gcatacccca aaccaaagag ggttaatccc agaaaggtgg aagatcatcg gcaaacaagt
      481 agccaatgta ccttttaaaa tatccataag caggacaaca atcccggctg ttttgcctaa
      541 tacacgaaat gtattggtcg ttccagtatt accgctcccg tgttgtcgga tatctttttt
      601 gaaaaacaat ttgccaatcc agacaccgga aggaatagaa cctaaaagat atgctaaaat
      661 aagtaataag atcaatttca ttttttaccc tctttcaagt tgaaaactat cctttatttt
      721 agcatgaata ggtaacagga acaattgaaa aaatgaaaga tacaagtata atagatggaa
      781 taaaaagaaa agcaaggggt ggcagaaatg tctgtaaaaa atccaacaca agaaacgatt
      841 ttcaactatt tggaagaagc aaaaaatatt gccgtcgtag gcttgagcaa taaaccagaa
      901 cgaacaagtt acatggttgc acaggtattg caagacaatg gatataaaat cataccagta
      961 aatccattat tggctggtga agaaattcta ggcgagaaag tctatgcgaa tttgacagaa
     1021 atcgatgatc caatcgatat cgtagatgta tttagacgga gtgaattttt acctgaaata
     1081 gcaggtgatt ttttgaaaac tgatgctaaa gtgttctggg cacaattagg gattgaaaac
     1141 aaagaagcag ctgagatgct aagaaaagca ggacgtacag atatcgtaat ggatcgttgt
     1201 attaagattg aacttggtaa gttgaaagaa aaccaaaaat aaaaatgacc acaaagaaga
     1261 gatgatcgat agaggggaat gtcgaaaaag cggttaggag ataattaaaa ttgatttctc
     1321 gtctaccctc ttttttttca cgaccttttc tagtatgata atcgtggtgc ggttttgccg
     1381 caaagtatta ttgaaggagc gtttgccttg gcaaaaaaag taaacaacga atacaatgac
     1441 tcgtccatcc aagtattgga aggattagag gcagttagaa aaagaccagg gatgtacatt
     1501 ggatccactg acagccgagg cttgcatcat ttagtctatg aaatcgtcga taatgcagtt
     1561 gacgaagcat tatcaggtta tggcagtgag attgatgtga ccatccatga agacaatagt
     1621 ataactgttg cagatagcgg acgtgggatg cctgtaggaa tgcatgcttc cggaatacca
     1681 acagttgaag tgatttttac tgtgctgcat gctggcggaa agtttggtca aggagggtac
     1741 aaaacttctg gtggactgca cggtgtagga gccagtgtgg taaatgcctt atcgaaatgg
     1801 ctaattgtaa cgatcgttag agatggcgta gagtatcagc agaaatttaa aaacggcggt
     1861 aaacctgacg gtactttgaa aaagatagga aaaacaaaaa aagcaaacgg gacgactgtc
     1921 cattttttac cagatgatac aatattcagt acaacgaaat tttcatatga aattttagcc
     1981 gaacggttaa gagaatctgc ctttcttcta aaaggcgtta aaatctctct ttctgatctt
     2041 cgtggagaag aaccagtcaa ggaaattttt cattacgaag aaggaatcaa agaatttgtt
     2101 gattatctga atgaagaaaa agatacattg acaccagttg tttatttttc aggagaaaaa
     2161 gaaggtatcg aagtagaggt cgcttatcaa tacaatgacg gttattcaga aaatgtttta
     2221 tcttttgtca ataacgttcg gacaaaagat gggggaaccc atgaagctgg tatgaaagcg
     2281 gcgatgacga aatcttataa tgaatatgcg agaaaagttg gtttgctgaa agaacgggac
     2341 aagaatctgg aaggtagtga tttcagagaa gggttagctg ctgtactttc tatccgtgta
     2401 cctgaaaatc ttctgcaatt tgaagggcaa acaaaagaaa aacttggtac gccagttgct
     2461 cgtaccgtag tagataacgt catcagtgaa caaatgggct tttatttgca ggaaaatagc
     2521 gaaatgagcc agatgttggt ccgtaaagca atcaaagcca gagaagcgag agaagcagca
     2581 cgtaaagcca gagaagaaag ccgaaatggg aagaagcgta aaaaaggcga atctcttctt
     2641 tcaggaaaat tgacacccgc acaatcccga aaccctaaga aaaatgaatt gtatttagtc
     2701 gaaggggatt ctgctggcgg atctgctaaa caaggaagag acagaaagtt ccaagcaatt
     2761 ctacctttac gaggaaaagt catcaatacc gaaaaagcta aaatgcaaga tatcttgaaa
     2821 aatgaagaaa tcaatacgat gatctatacg attggtgcag gagtcggtcc tgagttttct
     2881 atcgaagact gtaattacga caaagtaatc atcatgaccg atgcggatac agacggcgcc
     2941 catattcaag tattactgct tactttcttt tatcgttata tgaaaccgct gattgaagct
     3001 gggaaagtat atatcgcgtt accgccttta tataaagtat ccaaaggaca aggaaagaag
     3061 caagtcattg aatatgcgtg gacagatgac gaattggcag caatgattaa aaaagtcggc
     3121 aaaggatata tgctgcagcg atataaagga cttggagaga tgaatgcgga acaattgtgg
     3181 gaaacaacga tggatccgac ttcccgtacg ttgatccgcg tccgtatcga tgatgctgcg
     3241 caagctgaac gtcgagtaac cacactgatg ggtgacaaag tagaacctcg aagaaaatgg
     3301 atcgaaaatc acgtgcaatt tacgctcgaa gaagatggca gtatcttaga taaaaaagag
     3361 gatactgaaa tctctccttc agtttctaat gatttattag atgaagaacg agcagataaa
     3421 aatgaaaaca atcagttatt tgaagtagaa taggaggtca cctgatggaa aatcgacaag
     3481 aagtacaaga attaacttta gaagaagtga tgggtgaccg cttcggacgt tattccaaat
     3541 atattatcca agaacgtgca ctgcctgata tccgtgatgg attgaagcca gtgcaacgcc
     3601 ggatattatt tgccatgaac aaagatggca atacgtatga caaaggattc cgtaaatcag
     3661 caaaatctgt cggaaatatc atggggaatt atcatcctca tggtgaccgc agtatttatg
     3721 aagcgatggt ccgcatgagc caagactgga agttgcggga agtactgatc gaaatgcacg
     3781 ggaataacgg gagtatggac ggtgatccac ctgctgcgat gcgttatact gaagccagat
     3841 tatccaagtt aagtggagag atgctagcag atatcgagaa agaaacagtc gatctcgtgt
     3901 ggaattttga tgatacagaa aaagaaccaa cagtccttcc agctcgatac ccaaaccttc
     3961 tcgtcaatgg atcgacaggt atatctgctg gatatgcaac agaaatcccg actcataact
     4021 tagctgaagt gatcgatggc acaatctata tgatcgatca tccacaagcg agccttgaaa
     4081 aattgatgga atatattcct ggacctgact ttccaacagg agggatcttg caaggaaaag
     4141 atgaaatcaa aaaagcttat gaaacaggac gaggaaaagt tattcttcgt tcgaaaacaa
     4201 agatagaggc aatgaagggt aataaacagc aaatcgtgat cacagaaatc ccatatgaag
     4261 taaataaagc gaccttggtg aaaaaaatgg atgagatccg tttgaataag aaaatcgatg
     4321 gaatagctga agtacgagat gaaagtgata gaacaggtct gcaaattgtc gtcgaattga
     4381 aaaaagacgc caatgctcaa ggaattttga attatttgtt caaaaacact gagctacaga
     4441 tcaattacaa tttcaatatg gtcgctatcg accatatgac tccgcatcaa gttggactga
     4501 aagacatttt aagaagttat atcgagcatc gaaagcaagt cataacaaaa cgtagtcaat
     4561 tcgatttagc caaagctcaa aaaagacagc atatcgtaga aggattgatg aaagctttat
     4621 cgattctgga tgaagtaatc gcaacgattc gtgaaagtaa agacaaaaaa gacgccaaaa
     4681 agaatttagt cgacgtcttt caatttacag aagaacaagc agaagcaatt gtcacattgc
     4741 aattataccg tttgacgaac acggatatca cagagcttca aaaggaatcc gaaagcttga
     4801 ttgcgcaaat cacagagttg aataaaatcc tttcaaatga caaagagctt ttctctgtta
     4861 tgaaaaaaga attgcgagaa gtgaaaaaaa attattcttc cgctcgtcta acgacaatcg
     4921 aagacgaaat cgaagaaatc aaaattgata cgcaagtctt agtggcacaa gaagacgtaa
     4981 ttgtcagtgt gacgcgagaa ggctatgtga agcgaacaag ccttcgttct tatagtgctt
     5041 ccaaaccaga agaaatcggg atgcgagaag gcgactactt gctatactca ggtgaattga
     5101 gtacattgga ccatgttctt ctgatcacaa ataaagcaaa tgtgatctac cgccctgtcc
     5161 atgaactacc cgatttgaaa tggaaagatg ctggtgagca catttctcaa acaatctcca
     5221 atctgtctgt agacgaatcg attttagctg ttttcccata tcaaaaaatt caagcagaga
     5281 aaacatttgt attcatcagc aagaatggat tgatcaagca aacacgaatg acagagttcg
     5341 aaccatggcg cacctataag agccgtccgc tcagctgtat gaaactaaag gcggaagaag
     5401 atgaactgat tgctgtgtat ctgaaagaag atcagccagg cttagatgtt ttcttagtaa
     5461 cgcatcaagg aatgggtttg cgctatcctt taacagatgt tcccgttgtt ggagcaaagg
     5521 cagctggagt gaagtctatg aatttgaaag atggagatag gattatcaac gggctgcttg
     5581 tgttagcaga aggtgatacg ccgatcgtaa tcgttactca gcgaggagct gttaaacgca
     5641 tgcttgctca agaaatcagc caaacttcac gtgcgaagcg gggagtgacg gtcatgcgtg
     5701 agctgaaaaa aaatccgcac cgtattatct atatgtcgga aggaagatca aaagaaataa
     5761 ccttgatgaa tcaaaaaggg cagcagttaa cgattgaccc aactgatttc cctataggtg
     5821 atcgaacatc gaatggatca tttgcgatgg atgagaaaaa aggtggagaa gtcatcgaag
     5881 ttatcg
//
LOCUS       DACEXQ010000170         5626 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.170, whole genome shotgun sequence.
ACCESSION   DACEXQ010000170 DACEXQ010000000
VERSION     DACEXQ010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.170"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(172..486)
                     /locus_tag="IXH04_002182"
     CDS             complement(172..486)
                     /locus_tag="IXH04_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAQ1852754.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(500..760)
                     /locus_tag="IXH04_002183"
     CDS             complement(500..760)
                     /locus_tag="IXH04_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAQ1852755.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            930..1244
                     /locus_tag="IXH04_002184"
     CDS             930..1244
                     /locus_tag="IXH04_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ1852756.1"
                     /translation="MNLLFQSIKSITVPELKEKILENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            1246..2901
                     /locus_tag="IXH04_002185"
     CDS             1246..2901
                     /locus_tag="IXH04_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ1852757.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            2984..5119
                     /locus_tag="IXH04_002186"
     CDS             2984..5119
                     /locus_tag="IXH04_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1852758.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            5294..>5626
                     /locus_tag="IXH04_002187"
     CDS             5294..>5626
                     /locus_tag="IXH04_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852759.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFE"
BASE COUNT         1909 a          971 c         1142 g         1604 t
ORIGIN      
        1 aagcccaacc aaacgaaaaa tatgagaagc tatttttgtt tggctgttct tattacttga
       61 tgcaggacag cgttttcaca acctttgatt cacggttttc cagaagtagc ttcttcgaaa
      121 aataagccga aatttcttga agctgatcac ttctgtcgca acctctactg actaaggctt
      181 gatataagca taaccttggg attgcagttc aactaaatca aggacacctg ctggtacaac
      241 gattacttgt tcaggtaaag aactttctga aatatgatta gcacgcatag cattggcgca
      301 agcgtgaaaa acgactcctt gcaaatctaa gaattctgca ttggcagagt ctagataacc
      361 ggtaatcgcg atgccattta cagatacgac aatcgtcgtt tctggagaag cttttatcag
      421 gttttttacg tttttccctg tttctgacca tttttctaat tcatcaatat ggaaaacgac
      481 tttcattgaa atcattcctt tactttttaa taatcatctt gatcgcttgt tctagtttag
      541 ccgcttgttc ttctggatta ctatcaggtt tctcaaagca atttttcaga ttatctgcaa
      601 cgatcatgcc cattacgcga tcaatactag aacgaacagc gcttaattga gtgatgacat
      661 ccatacattc tttttcgtct tcgatcattt tttggatacc acgaatttgt ccctctgtac
      721 gtttcaaacg gttcaggata tttttattat caatctccat aacacattct cctctatttt
      781 tataattaga taatacccct tatcggtatt ttatgcaaat ataaaaagta gtgaaaaaaa
      841 tgaagagtca ttctggttga catattaata aagctcctgt ttaatatacc ccatgaggta
      901 tttataaaaa tgtaaatgag gtgcagaaaa tgaatttgct atttcaatca ataaaatcga
      961 taacagtccc agaattaaag gaaaagattt tggaaaatcc agcgttactt gatgttagaa
     1021 cacctgcaga ataccggggc gggcatataa aaggagctaa aaatgttcca ttacaaagta
     1081 taaacagata tgatggagac aaagaaaaga ccgtctatgt gatttgtcaa tcagggatga
     1141 ggagtaaaca agcagctaaa gaattaaaga aatctggtta tgatgtagtg aatgtacgtg
     1201 gtggcatgaa tcaatggttt gatcgaacag taggagggaa ataagatgaa aatcgttatt
     1261 gtcggaggtg tagcaggtgg aatgtctgct gctacacggc ttcgccgatt aatggaagat
     1321 gcagaaatcg ttgtttttga aaaaggaccg tatgtttctt ttgcaaattg cggcttgcct
     1381 tattatcttt cgggagaaat cagtgaacgg gaaaatcttc ttgtccaaac gccagaatca
     1441 ttatccgctc gtttttgttt agatgtgcgt ccaaatcatg aagtgacagc catctttccc
     1501 gaaaacaaaa cggtagaagt cgtacatgag ggtcaaaaac acattgaaca gtacgatgca
     1561 ttggttttat ctcctggtgc aaaaccagtt gttccatcga ttccagggat aacagaagcc
     1621 gacaatgttt tttctattag aaatgtacca gatatcgata aagtgatgca tgcattagaa
     1681 aaacagccaa agcgtgccgt gatcgttggt gcaggattca tcggattgga aatggcagaa
     1741 aacctaaaaa gaagaggttt agaagtcatg gtgatcgaac aagcaccaca tattcttcca
     1801 acactggatg aagaaatggc agcttttata gaaaaagaat tgtctcatca aggagtagaa
     1861 gtgattactt ctcatgctgt cgctggattt gaagaccacg ggaaacgatt gcgactggat
     1921 gatgggcgta ccatccctgc tgatttagtt attttatcca ttggtgttcg tcctgataac
     1981 cagctagcag tgaccgctgg aatcgaatta ggcatacgcg ggggtatcct agtagacgaa
     2041 cgttatcaaa cgaatattcc tgatatttat gcggtggggg atgctatcgt tgtaaaacag
     2101 caaatcactg gaaaagatgc acttatttct cttgcttcac cagccaatcg tcaaggtaga
     2161 caagttgcgg acacgatttc cggaatttct cgaagaaatc aaggcggtat tggaacagca
     2221 attatacgaa cgtttggaat gactgccgca tccaccggtt taagtgaaag aacagccaaa
     2281 gaaaacgaac tgtcttttga agtcattcat gtatcaggaa aagatcatgc aagctattat
     2341 ccagaagcaa cagatatttt actgaagttg atcttccatc cagagactgg cgagatttat
     2401 ggtgcacaag gtgttggggc aaaaggtgtg gataaacgga tcgatatttt agcaacagca
     2461 atcaaagggc atttgacgat cttcgattta ccggaattag aattgacgta tgcaccgcca
     2521 tttggctcag ccaaagatcc agtaaacatg ctaggatatg cagcaatgaa cattgcagaa
     2581 gggcttagtg aaaccgtaca atggcatgaa ttgccgacag aactagcaaa aggaaaaatt
     2641 ttattagatg tgcgaacagc agaagaattg gaaaagggca aattcaagga agccaaacat
     2701 atccctttga atgaacttcg ggaccgatta aatgaattag acagccaaca agaatatatc
     2761 gtcagctgtc atagcggatt acgtagctat ctagcggaaa gaatcttgaa gcagtctggc
     2821 taccacgtaa aaaaccttga tggtgcattt tctttatatc aaactgtccg acctgaagaa
     2881 ctgatatatc ctaacaaatg atactcgtta aaaagcttgc attcgactct taaaagaggt
     2941 aaactaatcg gttccaccac tttaaaaaaa gaggagaatg tgtatggtca acacacaaaa
     3001 acagttagaa gaagcatatc tgaagaacgt gtatcaaaag ctaattgata aaaaaaacga
     3061 actgcgggaa tcgatggata cggcaaaaat agaaggaaac cagtcgttga aagaaatggc
     3121 tggcgagctt cgattgaatt ttgacagtta cttagataac ctggatactt tttcgatgat
     3181 tgaaatgaaa aatagagaaa tcgatcagat gaatatcaaa cagaaaaacg cagaaaatca
     3241 gctagcaaag atcgaacgtt tattgaaaag tccctacttt ggaaaagtcg ttgtagactt
     3301 tctggataat gagccaaaag aatcctttta tatcggtaca gcaaatttca cagatgatgc
     3361 agatgaaaat cttgtttatg attggcgttc accaattgcc gaactttttt acaataacat
     3421 gattggtcca tcttcttata cggttcgtca aaataaaatc gatacggtta ttaaagaaag
     3481 acgacaattt cttttggaaa gagatcgttt ggtcaagttt tttgatacgt ctgtagctat
     3541 tcaagatgac gtattattag aagtcttagg ccaagatgag accaatgaaa tgaaggctat
     3601 cactgctacg atccaatcgg aacaaaacac aatcattcgg gatgtgaaaa gtcaaaatat
     3661 cttggtaaat ggcgtagcag gaagtggtaa aacctctgct atcatgcagc gtattgcgta
     3721 tctgctctat ttatatcgag atacgatgac atccgatgac ttgctgattc tatcacctaa
     3781 tcatcggttt atcgactata tttcgaatgt cctaccatca ttaggtgaaa gaaatccact
     3841 gaatctaacc atcatacagc tggtttcaca gctttcagca gaggaaatag aaggtgaaga
     3901 ggcttatttc aaacgtatca cgggagaaaa tgtatcggag cagacagaac gtttgcgtag
     3961 caaaaagttt atcgataatc taaaacaatc agatcctttg tttttagatc atccgaattt
     4021 tatccgtgga cttactaaaa atggaaaaac agtcctttct aaaaaaacga tcgaaaaaat
     4081 atatgagaaa gttcctgctc atcctaaatt gatcgatagg cttcaagcaa cgaaaaaggc
     4141 actgatgagc gaatggaaga atcatctttt gaaacaagca aaaagtcctg ctgtgcagaa
     4201 tcaggtactc tcgttgacag aagataggca gttggaatta ttcggtaaat tgatttctga
     4261 tgattcggaa caaagtattg tagcttatgc aaggaaactt ttacaaaaaa aatatcgaaa
     4321 aataaccaga caaatcgaag aaatggcatg ggtggatgag catcagttgt ttgaaagaat
     4381 ttatgaaaaa cgatatggct cagcttatgc ttggcaaccc acacgtactg ttgatgaagc
     4441 agtcattata ttagctattc gtcatttact tgttgaaaag gtaaatgtac cagcatttcg
     4501 ctatctattg attgatgaag tacaagatta taccctggca caattaggtc tattgatcga
     4561 attatttcca aaaactcact ttactttagt aggtgacgag aatcaggcaa tctttaattc
     4621 ttccactact tttgctgata ttatgcgttg tttcgatgac taccatctgc cgatccatcg
     4681 gtatgatctt agaaatagtt accgttctag cggagcaatc acagagcttt tcaaaaccta
     4741 tgctgtggat caagagaaga tcgacatcgt atcgatccga ccgcaaggaa aagaacccga
     4801 ataccgctct tttcgatcct ctgaagaatt gttgaaaata ctagctgaga ttttggtttc
     4861 tctaaaagga gaaaaagcgg cagttatcac ccaaacagaa gaggaaaccc aaacgctaca
     4921 agaatcgatt aaaaaaaatt catatggatt agaaaactgc caaatcattc ctttgagtct
     4981 agcaaaagga ctggaatttg atcatgtaat cttatatcca tttgaaaacg atggagatga
     5041 gcaaagaaga cgcagacaaa tgtacacggc gatatctaga ggaatgaaaa gcattgtcgt
     5101 attggaaaga gcaacataat taagagataa aaaataaaaa gaacaactga taaaaagaac
     5161 aaaatatttt tcatttgctg atgagaaatg ttttgttctt tttcttagga aaaatttagg
     5221 aacactttat gtgaaaagaa atgaaatgta tggtaaactc ctacgtcgta acaaaaaaac
     5281 gtgaggtgag gatatgaaaa aagtaacaag agaagaagtt gcatcaattt tggtgaaaga
     5341 taagtatttt tcaaaaggta attactggtt aaagatttgg caaacgttag tggctctttt
     5401 aggatggatg atcgttgttc ttccttttat atgggtcttt ttcccattaa cgagaccaga
     5461 aagagccaag gatttttcct tgtacgcttt tttatcagaa aagaaaatgc tgtatttctt
     5521 aatcggcttt tttgtattga tttttttcag tttcctgatt acctcatttg tattgactag
     5581 gtggaacaat cgaaatctat acaaaaaagt aaataaatct tttgaa
//
LOCUS       DACEXQ010000171         5540 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.171, whole genome shotgun sequence.
ACCESSION   DACEXQ010000171 DACEXQ010000000
VERSION     DACEXQ010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.171"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(176..1159)
                     /locus_tag="IXH04_002188"
     CDS             complement(176..1159)
                     /locus_tag="IXH04_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAQ1852760.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(1164..1811)
                     /locus_tag="IXH04_002189"
     CDS             complement(1164..1811)
                     /locus_tag="IXH04_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1852761.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(1896..2528)
                     /locus_tag="IXH04_002190"
     CDS             complement(1896..2528)
                     /locus_tag="IXH04_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1852762.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(2544..3095)
                     /gene="coaC"
                     /locus_tag="IXH04_002191"
     CDS             complement(2544..3095)
                     /gene="coaC"
                     /locus_tag="IXH04_002191"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAQ1852763.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(3092..3829)
                     /locus_tag="IXH04_002192"
     CDS             complement(3092..3829)
                     /locus_tag="IXH04_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAQ1852764.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(3826..4815)
                     /locus_tag="IXH04_002193"
     CDS             complement(3826..4815)
                     /locus_tag="IXH04_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAQ1852765.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            5073..>5540
                     /locus_tag="IXH04_002194"
     CDS             5073..>5540
                     /locus_tag="IXH04_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1852766.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     GTFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQAT"
BASE COUNT         1626 a         1100 c         1095 g         1719 t
ORIGIN      
        1 agtaaagaac acttttgtct cacgctctaa acacgtataa acggcggaag cagaagaaat
       61 tccttcggaa ataagctgaa attcacaaaa atttgaaaag caattttcgt gaatttcttc
      121 ttatttcttg gagttaaacg cttctgtccc agcctctttt ttgatcagca tgattttatt
      181 cagttatctt caaaatgatt tttccgatgt ttttgtttgc ttccatatat tcatgcgctt
      241 gtttgactgc gctgaattgg aatgtacggt caatgattgg ccgaatcttg ccattttcta
      301 gaaaaggccc agctttttgc ataaaagttt gtgcgagctc tgctttatac tgatcacttc
      361 taggtgtcaa taatgtacca agcagttgga cacgtttccc aatcaataca ccaaggtcta
      421 tctcaggcac gatggtccca cctagcatac caatcaagac ccaacgtcca tcaacggcta
      481 tagaagctaa attcttttcc cagtaagaag cgccgatgaa atccaaaata acatcgactc
      541 cccgaccttc agtgattttt cgtacctctt cttgaaagtc ttgtttttta tagttgatca
      601 attcatctgc accaagttct ttacaaaagg cgagtttttc ttctgatcct gcagtaacga
      661 tgattttcgc gtgagaaagc tgtctagcta attggatcgc tgctgttccc acaccacttg
      721 ctcctgcatg gatcaagaca gtttcgcctt cttgtaattt tccaagccag taaagtgttt
      781 gataagcagt caaaaaaact tctgagattc cagcagcaga aacatagtct agcgtatcag
      841 ggagaaggat tgcacgatcg gcaggcatca cagcatattc ggcataacca cctagattga
      901 ctaatccgta tacttttgtc ccaggagtaa aagtggagat atcactttta ttctcaacta
      961 cctctccagc tacttcgact cccaaaatag ggtagggagg tttcaaagag actgattctc
     1021 ttttcataat atctgttcga ttgacagctg ctgtatggac tttgatcagt aattcgcctt
     1081 gttttggttt aggaacaggc acttcaattt gttctaatac atctacagaa ccaggatgct
     1141 tgattgaaat ggcacgcatg gatctattcc tcctttgccc attcttggaa tgctgtattc
     1201 aactctttgc taccttcaga aagtactaga gtcgtacgag aaatatccgt ttcttccact
     1261 agagcggcaa gtacagcagc aactgtagag cgagtgatcg tttttgatgc cttctcatta
     1321 ggcttagtta attgaatgga agtaacttct tcatcgttag tcaatgtgac tggttgaaga
     1381 atgacaaaat ctaagttagt tcgtttcagc cattcatctg cataatgttt agtaatataa
     1441 taatctgtca tggactcagg ccattttgct ggttcatctg cataaacagc actgaccatc
     1501 agatagcgag agacatgcgc attttcagct gcgatcactg ttttgattgc gccatccaag
     1561 tctacttgca gaagattctt tccttgagag ccggcagcaa aaatcaatac atcgatgcct
     1621 ttgaatgctt cggtcatttt ttccacggac caagttaaat caaaagagac atacgaaaca
     1681 ttctcttttt ttactaatgt ttgggaagca acatctctca cccctgcaaa aatttcatgg
     1741 gaacttggag ctaaatcttt tatcaggtgt cggccaattt gtccgtttgc acctacaaca
     1801 aaaattttca tttggatcat cctttcgacg tcaaatcttt ccattcaata gtaacaccaa
     1861 aagagaaaaa aatcagagat cggctacgat gactatcagt tagtaagaga agggtgcttg
     1921 aagtatctta acaagacatt gcatactgct gcagtcgcaa gacctgcgac gattccttca
     1981 ggtataccac ttgtaaaaat aaccgctaga atagctgaat aaacagcatt taaatttgtt
     2041 ccgatgactt tggcataatc ttgctggaat aaaaagtaga tcatattcat cactaaaatc
     2101 gtattggtca atgaaccgga aagtccagct aagaataaag aaacggaatc tgctttttgt
     2161 ttcattagtt tgcgcagacc tttataaacg aaataaggaa ccaccccaat caagatgcga
     2221 ggaataaacg cgatcagcaa ggctttccag cttccatgtt ctgttccgat gactgggata
     2281 aacggagaaa agacaaaaga caaaggtgtt tggataactg tgctacggat catgctgatc
     2341 agtccaaatg taccgccaag aaaagcacct agtcttggtc ctaaaacgat cgaagcaatg
     2401 attacaggaa tatgcatggt tgttgcattg attggaccga tagggataaa acctaaaggc
     2461 gtaacagcta ataaaatcaa aatagctaaa aacatcgcag ttaatgtaaa gttttttgta
     2521 tttttcataa gtactccttt tttctaagca tttctttcat taagagtcag catgacctgt
     2581 tcgatgatcg tctgaatatc agctaaggca ccttttccaa aatctccaca agctaaaaga
     2641 gattctcttg gttcaatttc ctgatagccg acagatctca atgtttccag attacgctga
     2701 gtgatcgggt tttgatacat attcgtgttc atggcagggg caatcaattt tggtgttccc
     2761 actggaagcg ccattgcgac tgttgaaaga agatcgtctg caatgccgtt tgctagcttg
     2821 ccgataatgt ttgctgtagc aggcgcgatg agaaacaagt cagcttgttt tgctagttcg
     2881 atatgattga ttacctgcgg atttgattct tgcatgacgt ctgtatggac tgcgcgcttc
     2941 gacagagatt gcaaggttaa tggcgtgata aaatgtgtgc tgcttttaga cataatcaca
     3001 tctacctggg cacctaattt gacgaattga cttgtaagat cagcgctttt gtaagcagaa
     3061 atacttccag taacgcctag taagatcttt ttcattttga ttcctccagt ctaattttct
     3121 cagtaatcaa tgcagcaatc tcagatttac tttgagcttc ttccactgtt ccatctttag
     3181 ataagagata gccgtggtgt gtctcgccat ggatttccgt caaatcattt gcgaaaataa
     3241 aatcagtttt gttatttatc agactatttt ttgcgactgc cagaagagat tcctttggta
     3301 catctactaa tagtttgaaa ccaatgattt ttgcttttgg ctgccattca cgtagataag
     3361 aaatgatttt tggtgttttc ttcaaaacga gcacaagatg ctcggtgtta gaagagatct
     3421 ttgaaacagt ggtatctgtc tgttcatttt ccgcaaacca attgtccagt tcttcttgcg
     3481 ttgaagaagt gggtaggttt tttgccagct gttcttctga aaaactaaaa gcaggagtaa
     3541 aatcactgac tgccatactg tggataatcg catcgtagtg ttcttttttc gttagttctt
     3601 ctaattgtaa aaataagtcc agtgcagatt cgatcgtata aatcgttata ttggagtgtc
     3661 ttttcggttt cagtgcttgt ttcgtagtca cgtaatcgat tgtggtatcc ggattggcag
     3721 ctaaataatc agctaatgct tgccctaatc gtcctgtcga atgatttgta atagcacgta
     3781 cttgatcgat tttttcagtc gttcctccag ctgtgattag aatcttcatt tatggtcacc
     3841 tacatttact agtgttcttc cttggtgtgt tccagcaagt agtttagatg ctgtttcagg
     3901 caattgatcc agagtaatct cttggacgac cagttcatct gcaatctgca aatccgccat
     3961 ctgttgccat aaagaaagac gttgatctat tggtacattg acagaatcaa ttccaatcaa
     4021 ctggatgttt cgcagaataa aaggcaagac agtcgcgttt agtttgattc ccccagcatt
     4081 gccacataaa gctactgcac cgttataaga gatcaatgga agaagactgc ttagctgctc
     4141 gccacctact gtgtcgatga catagtcgat ttgctgtttt cctaaagctt ttgttgtctc
     4201 tggcagaaat tcatcaggtc gtacgacttg caaagcacct agacttttta gccatgtgac
     4261 agactcttct tttctggaaa aggcaatgat tgactggaat cctagtttat gtaacagggc
     4321 tatcgctgta ctgcctacac cgccagaagc accagtaacg acaacttttt tatcggaggt
     4381 cataccttga cggattaaag cgttcacaca caacatagca gtaaaccctg ctgtaccaag
     4441 gatcatcgct tgacgcagcg tcatattttt tggtagagga actaaccagt ctcctggtac
     4501 tttttgatat tggctataac cgcctggatg ggtgacgcca agcccaaaac cagtagcaag
     4561 tacaagatct tcttttgaaa aacgtggatt gttactttgg acgacagttc cggataaatc
     4621 aatccctgga gtcatcggat atgttcgtat cacctgacca gactcgctca ttgccaatgc
     4681 atctttataa ttgacatcag aataggcaat ttttaccaag acttcatttt cagccaactc
     4741 agtaaatggc agatcactga ttgctgtatg gaaaacaggt tcctttatta cctgaaaaga
     4801 tcgaaaagcg ttcattcgtg agacactcct tttaaaattt tcttttatca taccataaag
     4861 cctactcttt tattcttgtt cttccgtaaa tttagacaaa aattaggtga aaaataaaga
     4921 atcaaacaac tagaatttat tccaaaagat aaagaaatat tcagcaaaaa aaattttaca
     4981 aagggttgcc tttttcaata aatttcgtat actaatacga gtggtagtaa acaggaagtt
     5041 tactattaga aaacttcgat tcggaggaaa ttatggaaat tggtgagaag ttaagaaatt
     5101 tgcgcatcca aaagaatttg acacaagaag aactaggcga acgcacagat ttgtcaaaag
     5161 ggtatatttc ccagttggaa cgagatttaa gttcaccatc aatgggaaca ttcttttcta
     5221 ttcttgaagt cttaggtgtg actccagaag aattttttca tcaagaagca gcgaatctgc
     5281 aagtggttta ttcaaaagaa gatcatacgg tttatttcga tgaagaaaac gggtatgaat
     5341 tggaatggct cgtggctgat tcgaatgaaa aagaaatgga gcctgtcttg ctgacttttg
     5401 aaggtgcagg agaatataag acgtttgaac cttcattatc tgaaacgttt atttttgtat
     5461 tagaagggga attagagtta agcttaggag agacggtcta tgtggcaaaa gcaggacagt
     5521 cgatctatta tcaagcgaca
//
LOCUS       DACEXQ010000172         5519 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.172, whole genome shotgun sequence.
ACCESSION   DACEXQ010000172 DACEXQ010000000
VERSION     DACEXQ010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.172"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="IXH04_002195"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="IXH04_002195"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ1852767.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            2714..2938
                     /locus_tag="IXH04_002196"
     CDS             2714..2938
                     /locus_tag="IXH04_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852768.1"
                     /translation="MSGTYLSIYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFL
                     IIAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            3055..3552
                     /locus_tag="IXH04_002197"
     CDS             3055..3552
                     /locus_tag="IXH04_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ1852769.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSISELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMSDVARYYIEETGALGEVPASLQNYIDYESYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            3639..4031
                     /locus_tag="IXH04_002198"
     CDS             3639..4031
                     /locus_tag="IXH04_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1852770.1"
                     /translation="MKKIRSYTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAV
                     MILGNLPPLSMIEGAFLKYFGIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRRKLTY
                     AGKKVTLGRNQPQEAITAVRSEFYGISN"
     gene            4015..>5519
                     /locus_tag="IXH04_002199"
     CDS             4015..>5519
                     /locus_tag="IXH04_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HAQ1852771.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSILSAQERSKNEVTGKLKTVAYDKIDQQTDALISM
                     IGENQVDYRFFIGFKLLLNDQEFSMKSLTVEAKNAFTDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYPLSKKKLEH
                     ETLIKTYDLIKPTRCLVEEKQRYLKIQQEDETAYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKA"
BASE COUNT         1912 a          943 c         1154 g         1510 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatcag gaacttacct atccatatat aactggtttc tatcaaacgc
     2761 tcaatcactg gtgcttttag caatcgttgt gattggcttg tatcttggct tcaagcgtga
     2821 gtttagcaaa ctgattggct ttttaattat tgcgattatt gcggttggct tagtcttcaa
     2881 cgctgctgga gtaaaagaca ttttactaga gctattcaat cgcattattg gtgcttaaat
     2941 aaaaccgttc ttttgtggaa tataagtggt tttcttatgt tccgcaaagg aatggtacac
     3001 caaacgaagt gcggtaggga tttttgaatc tctacaaaga aaggacgtga atatatggac
     3061 gatatgcaag tctatattgc gaatttaggc aaatacaatg agggcgaatt ggtcggtgcg
     3121 tggtttacct ttcccattga ctttgaggaa gtcaaagaga aaatcggctt gaatgatgaa
     3181 tatgaggaat acgccattca tgattacgag ttacccttta cggttgacga atacacttct
     3241 attagcgaac tcaatcgcct atgggaaatg gtatcggagt tgccagaaga actacaatcg
     3301 gagctatctg ctctgctcac tcatttttca agtattgaag aactaagcga acatcaagaa
     3361 gatattatca ttcattccga ttgtgatgat atgtctgacg tggcacgcta ctacattgaa
     3421 gaaacgggtg ctttaggcga agtgccagcc agtcttcaaa actatattga ttatgaatcc
     3481 tatggtcggg atttagacct ttcgggaacg tttatctcaa ccaatcacgg gatttttgaa
     3541 atcgtctatt aaatctgtcg gtacattctt actggcagat tttcttgttt acggggtagt
     3601 caaaagacta tccccatttt tatgaaagga ttgattacat gaagaaaata cgaagctata
     3661 ccagtatctg gtctgttgag aaagtgttgt actccatcaa tgactttaga cttccgtttc
     3721 ccataacctt tacgcaaatg acatggtttg tcgtgtcact ctttgcagtt atgatacttg
     3781 gcaacttgcc ccctctttct atgatagagg gagcatttct caaatacttt gggattcctg
     3841 tggctttcac atggtttatg tctacaaaaa cctttgatgg taaaaagcct tatggatttt
     3901 tgaagtctgt cattgcttat gcactgcgac gaaagctgac ctatgcagga aaaaaagtaa
     3961 cgcttggcag aaaccagcca caagaagcca ttacagcagt taggagtgaa ttttatggca
     4021 tatccaatta aatatattga aaacaatctg gtctggaata aagatgggga atgttacgct
     4081 tactatgagc ttgttcccta caattactca tttctaagtc cggaacagaa aatacaagta
     4141 catgattctt tcagacagct tatcgcacaa aatcgtgatg gcaagattca tgctttacaa
     4201 atcagtacag aatccagcat actttctgca caagaacgtt ccaaaaatga agtcactgga
     4261 aagctcaaaa cggttgccta tgacaaaatc gaccaacaga cagacgcttt aatatccatg
     4321 attggcgaaa atcaagtgga ctaccgtttc tttatcggtt ttaagttgct tctcaacgat
     4381 caggagtttt ctatgaaaag tcttaccgtt gaagcaaaaa atgcttttac tgattttgtc
     4441 tatgatgtga accataagct gatgggcgat tttgttagta tgagtaatga tgaaatcctg
     4501 cgttttcaga agatggaaaa gcttttagaa aataaaatct cccgtcgttt caaaatccga
     4561 agattagata aggatgactt cggctatctg attgaacacc tttacggaca gacaggcact
     4621 gcctatgaag agtatgagta tcctctatca aagaaaaagc tggaacatga aacactgatt
     4681 aaaacctatg acctcattaa gcctactcgc tgtctggtgg aagaaaaaca gcgatatttg
     4741 aaaatccagc aggaagacga aaccgcctat gtagcttact ttaccatcaa cagtattgtc
     4801 ggcgaactgg acttcccgtc ctctgaaatc ttctactacc agcaacagca atttaccttt
     4861 ccgattgata cgtcaatgaa tgtggaaatt gtagcgaatc gtaaagccct atctactgtc
     4921 cgcaataaaa agaaagaact gaaagacttg gataaccacg cttggcaaag tgataatgaa
     4981 accagctcta atgtggcgga agctctggaa agtgtgaacg agctggaaac caatttagac
     5041 caaagcaagg aatctatgta taagctgtcc tatgttgtaa gggtatcagc aaatgacctt
     5101 gacgaactta aacgtcgttg taatgaagtg aaagatttct atgatgattt gagcgtgaaa
     5161 ctggtacgac cttttgggga tatgctgggc ttacatgaag aatttttacc tgccagcaaa
     5221 agatatatga atgactatat tcaatacgtg acctctgatt tcctcgctgg tttaggtttt
     5281 ggtgctactc aaatgctggg tgaaaatgag gggatttatg ttggctacag cttagatact
     5341 ggacgcaatg tctatctgaa acctgctctt gccagtcaag gggttaaggg ttcagtaacc
     5401 aatgcgttag cgtctgcctt tgtcggttcg ctgggtggtg gtaaatcctt tgcgaataac
     5461 cttatcgtct attatgcagt gctttatggg gcacaagcag tgattgtaga cccaaaagc
//
LOCUS       DACEXQ010000173         5463 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.173, whole genome shotgun sequence.
ACCESSION   DACEXQ010000173 DACEXQ010000000
VERSION     DACEXQ010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.173"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..933
                     /locus_tag="IXH04_002200"
     CDS             <1..933
                     /locus_tag="IXH04_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ1852772.1"
                     /translation="RWKETLPEISHEINIVNLTSDEKNKGLLDPYVIMKNTKDSESLA
                     IDILTFLTGISSRDGERFPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHI
                     ESFTDYDFAHLLFSDGYVEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAM
                     LIVISTFALDFIHTDRSIFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVT
                     QNTDDLLDEKLKNNLGLKFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCL
                     ISDLYGRVGVIQFHPVFEELLHAFDTRPPVRKEV"
     gene            936..3119
                     /locus_tag="IXH04_002201"
     CDS             936..3119
                     /locus_tag="IXH04_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ1852773.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            3116..4117
                     /locus_tag="IXH04_002202"
     CDS             3116..4117
                     /locus_tag="IXH04_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ1852774.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            4132..5046
                     /locus_tag="IXH04_002203"
     CDS             4132..5046
                     /locus_tag="IXH04_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1852775.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            5291..5407
                     /locus_tag="IXH04_002204"
     CDS             5291..5407
                     /locus_tag="IXH04_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAQ1852776.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
BASE COUNT         1727 a         1048 c         1154 g         1534 t
ORIGIN      
        1 agatggaaag aaaccttgcc agagatttcc catgaaatca atatcgtcaa tctgacttct
       61 gatgagaaaa acaaaggctt acttgaccct tatgtaatta tgaaaaatac caaagattct
      121 gaatcactgg ctattgatat tttgacattc cttacgggga tttcctctcg tgatggggaa
      181 cgcttcccaa tccttagaaa agccattcgt gcagtaacca atagtgaagt gcgagggttg
      241 atgaaagtga ttgaagaatt acgggttgag aatacgccac taagtaccag catagccgac
      301 catatcgaaa gttttacaga ctatgacttt gcccatctgc tttttagtga tggttatgta
      361 gagcagtcta tcagccttga aaaacaactg aacattatac aggttgccga cttggtactt
      421 cctgacaagg aaacttcctt tgaggaatat accacaatgg agttactatc ggtagcaatg
      481 ctgattgtca ttagtacctt tgcgttggac tttatccata cagaccgaag tattttcaag
      541 attgtagact tagacgaagc atggagcttc ttacaggtgg cacaagggaa aacactatct
      601 atgaagctgg ttcgtgctgg tcgtgctatg aacgctgggg tatatttcgt gacccaaaat
      661 acagatgacc tcttagatga aaaactgaaa aataacctcg gcttaaaatt tgcctttcgt
      721 tccactgacc ttaacgagat taaaaaaacc cttgcctttt ttggtgtaga cccagaggac
      781 gaaaacaatc agaagcgatt gcgtgatttg gaaaacgggc aatgccttat cagtgattta
      841 tatggtcgtg tcggtgtgat acagttccac cctgtatttg aagaactgct ccatgccttt
      901 gataccagac cacctgtgcg aaaagaggtg taaatgtgaa accatcaata ttaaacagaa
      961 taaaatcaaa ctggacgctg aaacgtctag gtaaagtggc aatgacagtg gctttcacac
     1021 ttgtgattgc catttttctt ttagccatgc tgggaacggt ggttcaagct gcgggcttgg
     1081 tagatgatac ggtcaatgtg gcaaatgaat acagccgata cccacttgaa aactatcaac
     1141 tggattttta tgtggataat agctggggct ggctaccgtg gaactggtcg gacggaattg
     1201 gaaaacaagt catgtatgga ctatatgcca ttaccaattt tatttggaca atcagtcttt
     1261 atgtttccaa tgcgacgggt tacttagtac aggaagccta ttccttagac ttcatttccg
     1321 ctacagcaga ttccattggc aagaatatgc agacccttgc aggggttacg cctagtggac
     1381 tatcaacaga gggtttctat gttggattcc ttttactctt gattttggtt cttggggttt
     1441 atgttgccta tacaggactg ataaagagag aaaccacaaa ggcaattcat gccctcatga
     1501 attttgtgct ggtttttatc ctatcggctt cctttattgc ctacgctccc gactacatta
     1561 aaaaaatcaa tgacttttca tcagacatca gcaacgctag tttgtcactg ggtacaaaaa
     1621 ttgtcatgcc ccattcagat agtcaaggca aggacagtgt ggacttgata cgtgacagcc
     1681 tattttccat acaggttcag caaccgtggc tactgcttca atataacagt tcagacattg
     1741 aaagtattgg tattgaccgt gtggaaagcc tgctctcaac cagtccagat tccaacaatg
     1801 gggaagacag agaaaaaatt gttgcggaag aaattgaaga tagaagcaat accaatatga
     1861 cgattacaaa gacgattaac cgtttaggta cagtcttctt cctatttgtc ttcaatattg
     1921 ggatttccat atttgtattc ctattaacag gaatcatgat tttctcgcag gtacttttta
     1981 tcatctatgc tatgtttctg cctgtgagct ttattttaag catgattcca tcatttgatg
     2041 gtatgtcaaa acgagccata acaaagctct ttaataccat tttgacacga gctggaatca
     2101 cattgattat tacgacagca tttagtattt caaccatgct ctatacctta tcggctggtt
     2161 atccgttctt tttgattgct tttctacaga ttgtgacctt tgcaggaatc tactttaagc
     2221 tgggcgattt aatgagtatg ttttctctac agagtaacga ttctcaaagt gtgggaagtc
     2281 gtatgatgag aaaacctcgt atgcttatgc acgctcacat gcaccgtcta cagcggaaac
     2341 ttggacgctc cgtgactgct ttaggggctg ggtctgccat tgccagtgct acgggtaaaa
     2401 aaggacagtc gggttcggga agttctgcga gtacacaagc agatcactct cgaccaaacg
     2461 ggcaggaaaa atcaacacta ggaaagcgta tcggtcaaac catcggtaca gtagctgata
     2521 ccaaagacag aatggtagat actgctggta atttgaaaga acaggttaag gatttgccga
     2581 ccaatgcaag atatgcagta tatcaaggaa aatctaaagt gacggataat gtccatgatt
     2641 taaccagaag tatttctcaa accaaagcgg ataaagccag tggacgcaag aaacagcagg
     2701 aacaaagacg gaaaaccatt gcggagcgtc gctctgaaat ggaacaggtc aaacagaaaa
     2761 aacagccagc ttcttccgtt catgaaagac cagccactaa gcaagaacaa tctcatgatg
     2821 gacagacttc aaggcaaaca actgtacagg cttcctatag ggaatctcaa caagccaaac
     2881 aagagcgtcc aacagttaag tccgattctt caagtttaaa aacggaacgt caaagtaata
     2941 cagttcaaga aagaacggtt caaaagccag taacttcaac tgcaccaaca gatagagctt
     3001 cacaacgttc aatcacaaaa gaacgtcagt ctactgttca aagagtacca ctacaaaata
     3061 caaaaagtag accaccaatc aaaaccgcca ccattaagaa aggcagtaag aaaccatgaa
     3121 gctgaaaact ttagtgattg gtggttcggg attattcttg atggtcttct cactgcttct
     3181 gtttgtcgcc attttatttt cagatgaaca agatggcggt ttttccaaca ttcactatgg
     3241 cggtgtggat gtttccgcag aagtgctggc tcataagcct atggtagaaa aagttgccaa
     3301 agaatatgga attgaagaat acgtcaatat acttcttgcg attatacagg tggaatcggg
     3361 tggtacagca gaagatgtta tgcagtcctc ggaatccctc ggtcttccac ctaattcatt
     3421 gagtacagaa gaatccatta agcaaggtgt gaagtatttc agtgagttat tagccagtag
     3481 cgaaaggctt ggtgtagatt tagaatcggt tatccagtcc tacaattatg gtggtggttt
     3541 cttaggatat gtggctaatc gtgggaataa atataccttt gaactggctc aaagtttctc
     3601 aaaagagtat tcgggtgacg aaaaagtgtc ttaccccaat cccatagcca tacctatcaa
     3661 tgggggctgg cgatacaatt acgggaatat gttttatgtg caactggtaa cgcagtatct
     3721 tgtcacaaca gagtttgatg atgatacagt acaagccatc atggacgaag ctctgaaatt
     3781 tgaaggctgg aagtatgtct atggcggagc ttccccgact acctcttttg attgtagcgg
     3841 actgacacaa tggacgtatg gaaaagctgg aatcaactta ccacgaacag cacaacagca
     3901 atatgatgta acccggcata tcccattatt ggaagcaaaa gccggcgatt tggttttctt
     3961 tcattctacc tataacgctg gctcttatat tactcatatc gggatttatt taggcgataa
     4021 ccgtatgttt catgcaggcg accctatcgg ttatgccgac ttaacaagct cttactggca
     4081 acagcattta gtgggagcag gacgaatcaa acaataagaa aggacgattc aatgatgaaa
     4141 ttcggaaaaa atcagaatac agaaaaacag acaccaaagg aaaagaaacc tcgtgtctat
     4201 aaggtcaatc ctcgtaaaaa ggttgtgatt gtcctatggg tacttttagg acttagtttc
     4261 agctttgcga tattcaagca ctttacagcc attgataccc ataccattca tgaaaccacc
     4321 atcatagaaa aggaatatgt cgatactcat catgtggaaa attttgtaga gaactttgcg
     4381 aaagtctact attcatggga actaaacgac aagtccattg ataatcgcat agaaaacctg
     4441 aaagcatatc tgacagagga acttcaagct ctgaatgtcg atacagttcg taaagatatt
     4501 cctgtatcgt cttctgtaaa aggatttcag atatggacgg tagaagcaga tggcgacaat
     4561 caatttgatg taacctacag tgtagaccaa ctcattacag agggggaaaa tacaaagacc
     4621 gtccactctg cttatatagt gagtgtctat gtagacagta ctggaaatat ggtcctcatt
     4681 aagaatccga ccattactag cataccaaag aaatcagact ataaaccaaa agctattgaa
     4741 agtgagggta cggttgattc cattacaacc aatgaaatca atgagttttt aacgacgttc
     4801 ttcaagctct atcctacagc gacagccagt gaactttcct actatgtgaa tgacgggata
     4861 ttaaaaccaa tcggaaaaga gtacatcttt caagaactgg taaatcctat ttacaatcgt
     4921 aaggataatc aagtcacggt atcgctgaca gtggagtata tcgaccagca gaccaaagca
     4981 acgcaggtat ctcaatttga tttggtactt gaaaagaacg ggagtaattg gaagattata
     5041 gaataacaaa tattggtaca ttattacagc tattttgtaa tcacgtactc tctttgataa
     5101 aaaattggag attcctttac aaatatgctc ttacgtgcta ttatttaagt atctatttaa
     5161 aaggagttaa taaatatgcg gcaaggtatt cttaaataaa ctgtcaattt gatagtggga
     5221 acaaataatt ggatgtcctt ttttaggagg gcttagtttt ttgcacccag tttaagaata
     5281 cctttatcat gtgattctaa agtatccgga gaatatctgt atgctttgta tgcctatggt
     5341 tatgcataaa aatcccagtg ataagagtat ttatcactgg gatttttatg cccttttggg
     5401 cttttgaatg gaggaaaatc acatgaaaat tattaatatt ggagttttag ctcatgttga
     5461 tgc
//
LOCUS       DACEXQ010000174         5451 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.174, whole genome shotgun sequence.
ACCESSION   DACEXQ010000174 DACEXQ010000000
VERSION     DACEXQ010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.174"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..111
                     /locus_tag="IXH04_002205"
     CDS             <1..111
                     /locus_tag="IXH04_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAQ1852777.1"
                     /translation="ISREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            complement(569..844)
                     /locus_tag="IXH04_002206"
     CDS             complement(569..844)
                     /locus_tag="IXH04_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852778.1"
                     /translation="MYKPYMISYDLNNPGQKYDKVFEIIKEFGAYIKLQKSFWLVKTN
                     LNPNQMCEKLNTVLDNNDSLFICELQKNYQGRATEENWKFINEHIFS"
     gene            1492..1659
                     /locus_tag="IXH04_002207"
     CDS             1492..1659
                     /locus_tag="IXH04_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ1852779.1"
                     /translation="MYRPQYLEQKYEVITVHKGNGEKVYEYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
     gene            1685..2029
                     /locus_tag="IXH04_002208"
     CDS             1685..2029
                     /locus_tag="IXH04_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAQ1852780.1"
                     /translation="MTEEFYRWLLQLTREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            2034..2315
                     /locus_tag="IXH04_002209"
     CDS             2034..2315
                     /locus_tag="IXH04_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852781.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            2418..2732
                     /locus_tag="IXH04_002210"
     CDS             2418..2732
                     /locus_tag="IXH04_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAQ1852782.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            2710..4404
                     /locus_tag="IXH04_002211"
     CDS             2710..4404
                     /locus_tag="IXH04_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAQ1852783.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            4424..>5451
                     /locus_tag="IXH04_002212"
     CDS             4424..>5451
                     /locus_tag="IXH04_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ1852784.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGV"
BASE COUNT         1929 a          762 c         1095 g         1665 t
ORIGIN      
        1 attagccgtg aagtgggtta ttcagaaaga agtatacaac ggatgaagtc ggaagctcta
       61 atagagtttg cagaagcata taaacacgga aaaataattg cttataaata atttggcggt
      121 tttttggcgg aatgatggcg gtttttagac atttaccagt gttattatgg tagtgtcgaa
      181 agattaggaa acaggatcga caaaataaac gaagggaggc aatctccctc atcgttttaa
      241 acttcttctt agatagacag caactaatta tgaagaaaag gatgtgaatt caactcctcc
      301 taaattcttc atcggttgct gtttattttg attagacagc agcactcaca aaactaaaca
      361 tgttcttgtc gtctttcaac tgctgctgtc tattaattta tgtattggag ggaagaaaaa
      421 tgaaattatc agtagaaggc actccagaag aaatacaaga attgctccaa gctattggta
      481 gtagcaagga gcaaaaggta agtgtttcta attcagatat tgacggtatt tgcaattgtt
      541 taaacagaga aaaaatatct aatccttact aagagaaaat atgttcgtta atgaatttcc
      601 aattttcctc tgtagctcta ccttgataat ttttttgcag ttcacagatg aataaggaat
      661 cgttattatc aagtactgta tttaattttt cacacatttg atttggattc aaattagttt
      721 taactaacca aaaagatttt tgtagcttta tatatgcccc aaattctttt attatttcaa
      781 atactttatc atacttttgc cctgggttat taagatcata actaatcatg tagggtttat
      841 acatgttgtc ttgcacatcc ttgttttata tttcagcgga ccactcgctg ataaataaaa
      901 ttatacgctt agtatttatt ttcacaatat taatttatcg taataacttt tgagtaaata
      961 gaacaaaaaa cctgcactag tttccgctag tacaggcagt gactatatcg gttgataatc
     1021 tagcatatat taaaaataat tgcaagaaaa gaaatttata gtggtttata ccaaattatc
     1081 aaagcaatgt tattttgttg ctgtctattg ttttttaatt attcacacga taactaaagg
     1141 tgggtgaaga gaaatgattc cattaataat ttcaattttt gcgctctgtc ttaatgtcta
     1201 tatgattgga tttaaaaatg ggcaaaataa aaaatagtag cagccaagaa taattttata
     1261 gtgtcactgt ggcggaaagg gtagacgcta agcatgtgtg ctaggtcaat gcttcggcaa
     1321 ccatgcaagg ttcgattcct tgccagtgac tttggcaacc gaggcatcgg cggtttaaaa
     1381 atataggggt gcgcaatttc gtacgcgttt tgtgcatcgt gtaggttgct attacatatt
     1441 agatcactct ttgagtggtc tttttatttt tgcacaaagg aggaaacaac aatgtataga
     1501 ccacaatact tagaacagaa gtatgaagta atcactgttc ataaaggtaa tggcgaaaaa
     1561 gtatatgagt atagaagacc aataaagagc gatacatata aacgaaagga aaacaatgaa
     1621 gttattccat tgtatggcaa aagaatagct aagcattaaa taagattgcg aaaggagacg
     1681 gaacatgacc gaggaattct atagatggct attacagttg acaagagaag atcgtttggt
     1741 taagttctat cagtctccta aatggcgcag gcttagagag aaagcgatga aacgagatca
     1801 ctatgaatgc caagagtgta gaagactagg taagtatcat agagtagaga acgttcatca
     1861 tataaaggaa gtcaaggata gacctgactt agctttagat ttagataatc ttatttgttt
     1921 atgtgttgaa catcataatg aagttcatgg cagatatctt acagcattag ataaacaaga
     1981 gaagaagata gaaagctttg ctaacttcga tgcaagtgaa aggtggtaag tgcatgatca
     2041 tcaatgataa tggcagagag tatgatacag aaaagattga agagtattca tcttatactc
     2101 agggattaat taaacgtttg atatacgttc gctatgtagg tattagggat ctgttatcag
     2161 ataactgttg tagtaaatac aaagtgaatc aagtaagaga agcgttgaat aaagataata
     2221 acgtcgaaag aataaaaaat gtttttggat atagtattga agagattaat tattacattg
     2281 acttcgctga agctttcatt ccgatggtga gataaccccc ccttaaaata aatcgcaaat
     2341 tttttggggg tgatgaaacg gagggggctg tcaggaaaag agattttttc gaactttatc
     2401 atgaaaggag ggctaaaatg tttaaaaacg aattgtctca aaatcgctac agagaaaaat
     2461 tacgccgctc tttaataagc caattggaaa gtcagaaaac aaatattgag ccattcttag
     2521 ataatgttga tcgttatatc agtttatggg aaacggcgat atcactggaa gaagatatat
     2581 ccgagaacgg cattagactg gagaatggta aaaagaatga atcagtagcg ttgcttgttt
     2641 ctgtcaacaa acaaatggga ttgatgttgg ataaacttgc cattactcct gaattggtag
     2701 gtgaagcaaa tgaatcaatt cctgagttat aagcatattg aaaattggtt caaagctata
     2761 gaagaaggca ctatcaaggt atgcaaagag caattattgc taaaaaatta tctagaagaa
     2821 agagtcttta ctagagaaga tatttacttc gataagcaga tggtagagga ttcaatcaat
     2881 ataccagcac aatactttcc attcgaatta attccgtggg aaaaatttct acaatgtttt
     2941 atttatggtg ttcgatggaa aaaagataaa acactagtgt tcaatagata tctttcatta
     3001 atgggacgtg gtaatggtaa aactggtttt gcttcttgga acaacttctt tctactaacc
     3061 gctaaacacg gtattaaaaa ttatgatatt gatatctatg ccaataatga aagccaagca
     3121 aagactagtt ttgatgatgt atttaaagta attaaagatc atcctgattt agataaaaaa
     3181 gtatttaaag ctacgaagga agttattcaa aatatcgcta caaacagcaa acttcgttat
     3241 aacacggcaa atgctagaac aaaagatggg aagcgaccag gtgcaaaccg ctttgatgaa
     3301 attcacgaaa atgaagatta ttcaatgata aatgtggcta cttctggtgg tggtaaaatt
     3361 cgagattata gagaatttta tgatacaact aatggtcatg ttcgtggtgg tccgcttgat
     3421 gacattatag aagaatcaaa aatgattctt tctggagaac ttggaattga caaggatgga
     3481 gcagaatttt ctagtttgtt tccatttatt tgtcgcttgg ataacgataa tgaagttgat
     3541 gatcccgaca tgtgggaaaa agcttgtcca actattaatt acaatgcaga tttaaaacgg
     3601 aaaatgtttc aagaatactc tcaaatgcaa cgtaatgctg gtttaagact tacgttcatg
     3661 accaaacgaa tgaacagacc tatggaagat acacgatttg ctgttgcttc atatgatgat
     3721 gttctgcata cgaaagaaaa agaatttcct gaaaaaatgg atgaagtgat aggaacagtc
     3781 gattttgctg atagacgaga ttttgccagc gttgggttgc taggaaaata cgataaagat
     3841 gtttatttta cacaacatac ttttatccac gaatcagccc ttcgattaca aaacatcaaa
     3901 cgagaggtta tagatatttc tatagatcaa ggaaaatcac agatcgttca tggaaaaaat
     3961 atagaagctg attatattgt aggttggttt cttgaaatga gtaataaata ttatattaaa
     4021 aaaatcgcta tggatatgta ccgtgcaaaa atattgaagc ccgctttaga agaagcaggt
     4081 tttactgtgg aaattgttcg aagcggatct gttacacatg gtatgttaaa agatctggtt
     4141 gatgaccttt ttattaatca acgtttattt tttggtgacg atgcgattat gcgttggtat
     4201 tgcatgaatg tatatgaaga gcatatttct aatggaaata tacgctatga aaaaatagaa
     4261 cctgaaacta gaaaaacgga tggctttttt tcattccttc atggtttgaa ttttttagat
     4321 gatatttatg attctgctcc tgtaacagtc acaaatagct cagtagaaaa tacaggaact
     4381 ggatttactc ctctagtatt ctaacttgaa aggaggtgag aaagtgggga tttttcaaaa
     4441 ggcggtagga tacttcacaa aaaaagcaac ggttccttta gaagaatact tttgtaaatt
     4501 gcaagttgat tttgtgtatc gaaaatttgc gattgaaact tgtattgatt tgattgcaaa
     4561 tgcgatgagt aaagcggaat tcaagtcata tgaagatgga aaaaataaaa agaatgatct
     4621 ttactatagg ctgaatgtag ctcctaataa gaaaaataat gcaacagaat ttagaaaaaa
     4681 actgatcagg agattaatat tctacaatga agtattgatc gtttctccgt ctaataattc
     4741 tagcgaaata tttattgcgg atagttggga tgtcacagaa tatgcattga aagatgatgt
     4801 gttttctcaa gtgcaaatta acaacatagt ccttgataga gaatttctag aaagtgatgt
     4861 tatctatata aaatacgcag atcaacaaat taggcaacta gtcgatgcgt attatcaagc
     4921 gtatgggaaa ctcatttcta gtgccatgaa tgtttacaag cgctctaacg ctcgtagata
     4981 cgtactgaaa gggaatttat tccgaccgca agacaataca acacaagatc aaatcaataa
     5041 aatgatgaca tcacaattta aggcttttat ggaagctgat aatgcaggtg cggtatttca
     5101 attacaaaat gagtacacat tagaagattt cagcggaaac ttccaaagca attcaagaga
     5161 tataaaaaac ttaatagacg acatctttga gatgacagca gcagcgtttc acgttccgaa
     5221 aaacctacta aagggagaca tgagtgggtt atcggatcaa gtggacgctt ttttaatgtt
     5281 cgaaatcata ccgattgctg aacttattca ggatgcgttt aacgctagtc tctatgaagt
     5341 agaagaatac ttgtcaggga attttgtacg tgtggataca actatgatca agattactag
     5401 cttcaaagat ttggttgacg ctattgatgt aggcattaga aatggagtat t
//
LOCUS       DACEXQ010000175         5394 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.175, whole genome shotgun sequence.
ACCESSION   DACEXQ010000175 DACEXQ010000000
VERSION     DACEXQ010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.175"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..859)
                     /locus_tag="IXH04_002213"
     CDS             complement(<1..859)
                     /locus_tag="IXH04_002213"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAQ1852785.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIK"
     gene            complement(971..1303)
                     /locus_tag="IXH04_002214"
     CDS             complement(971..1303)
                     /locus_tag="IXH04_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ1852786.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            1451..2326
                     /locus_tag="IXH04_002215"
     CDS             1451..2326
                     /locus_tag="IXH04_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1852787.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(2408..2989)
                     /locus_tag="IXH04_002216"
     CDS             complement(2408..2989)
                     /locus_tag="IXH04_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAQ1852788.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(2995..4254)
                     /locus_tag="IXH04_002217"
     CDS             complement(2995..4254)
                     /locus_tag="IXH04_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ1852789.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(4256..5302)
                     /locus_tag="IXH04_002218"
     CDS             complement(4256..5302)
                     /locus_tag="IXH04_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852790.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
BASE COUNT         1517 a         1115 c          927 g         1835 t
ORIGIN      
        1 ctttaatcat ttggttagaa cacttccacg atttcaaaaa ggaacgaatc tcattttctt
       61 ttagatccag ttggctgatc aataatgccc atgcttggct ctcttctttc aatgggacgg
      121 atggtaattc agcaaaacgt aaaagtgctt cgccttcttc tttcaaaccg ggacagcaaa
      181 tgtagcactc tgtttcaata aatgcatgga ttcccgcggc ccgttcattt cccaagagca
      241 tcttgatgaa ttccacattt atacgttcta cagatatttt acctaatagc tcgtggtttt
      301 ctttgatggc gtagaaggtt tcttcctcta atttgaaccc tagttgactg acaaaacgca
      361 acccgcgcat cattcgtaag gcatcttcat ggaaccgctc atgaggattg cctacagctc
      421 gcaagatctg ttcttctaga tcttgcagac catcgaacaa atcgatgatt tctccatctt
      481 ctttcaaagc aaacgcattg atcgtaaaat ctcttctttt caaatcttct gccaacgaac
      541 gaacgaattc gacatgatct ggtctacggt agtcctgata agttgattcc gtccgaaatg
      601 tggtgatctc atattgttca tcattttcta ataccaacac agttccatgt tcgatcccaa
      661 tatctatcgt ttttggaaaa agctgtttga tttcttctgg aaatgcgctt gttgcgatat
      721 ccacatcatg gattggctgg ttcagcaaga tatcacgaac acttccacct acaaaataag
      781 cttcatatcc tgcattctct aattttttca gaactggaat tgccttttgg tattccagag
      841 gtaattgttt taatttcatt tttccaacct ttcctgcgtg tttcttgtcc tgagataaag
      901 actcagttgc atggtgactg tttgacaagt ccgtttcaga gtcttcaagg ccaggaccac
      961 ttcatcttgt ttactcgtcc ttacgctcaa agcgattatg gtctctttga ttgaattttt
     1021 ccatgttttt ttcaaaaact tcagttaagt cgatattcaa tgaattagcc atgatcatcg
     1081 taacgaataa aacatcacca agttcttctg ctaccgagtt aactggttca gaagcctttt
     1141 tgcttttttc tccataagtg tggttgactt ctcttgccaa ttcacctact tcttcagtta
     1201 atcgagccat ctgagctaat ggagagaaat accccacctt gaattgctgg atatattcat
     1261 ccacttcctg ctgcatactg tataaagaac gctttttatc catctttttc cctccaaaac
     1321 tatcttacca aaactttaac gcaaatccta gataagagca attgagaaaa tcgaatttcc
     1381 atgctacatt taggaaggaa tttctaaatg gaaattctgt gaaaatacat aattgaaagc
     1441 tggaattcat atggtcaaac gaaaaacagt aaaagaagtt ctttttatca ttataggaac
     1501 aagtatttat gcatttggtc ttgtttactt gaacatcgct aatcatttag cagaaggggg
     1561 agtctcaggg atcaccttga tcttaagagc tttatttggt attgatccag cttatacgac
     1621 tttattaatc aacatcccat tgattttact aggaggaaag atcctcggta aacgttcctt
     1681 ggcctatact gttctaggta cactttcttt gtcattcttt ttatggatct ggcaaagggt
     1741 tccactagaa atcaatttac agcatgatct actgatcgtt tctctacttg cgggtttagt
     1801 tgcaggtgta ggaagcggga tcgtctaccg catgggtgga acaacaggcg gcagtgatat
     1861 tatcgctcgg attttagaac aaaattacgg catcactatg gggcgttccc tattggcatt
     1921 tgatatcatc gtcttacttg cttcattaac ctatatcgac ttgaaacgta tgatgtatac
     1981 gttgattgtt tcgtatgttt tcagtcgtgt gattgattcg atcttagatg gcggatattc
     2041 tgcaaaaggt ctacttgtcg tctccaacaa aagcgaagag attgcaccac tactgatgac
     2101 cggtctgcaa agaggtgtga ctttcctttc tggagaaggc gcattctctg gtaattcaaa
     2161 aaaaatcatc tatatggtcg ttagctctag tgaattaact gaagctaaga ggatcgtcca
     2221 cgagattgac gaaaaagctt tcctttcaat catcaatgtg catgaagtgg aagggcaagg
     2281 ctttacttat ttgaagccac agacaagatt actgaaaaaa ctataagaaa aaagacaaag
     2341 ataggctatg aggaaagatt ccacatccag ctgacctttg tcttttttag tactatacta
     2401 tttttaacta attgttatgt tcacttaccc ggttactttt cttttgtgtc tgcaaaagga
     2461 tctttcggtt aacttcatca gataatttca aaaaagcatc cctatctcca tcttctagtg
     2521 cttgatcgat ttgagcataa agcattttta gttttgcctc ttcttctttt tcttgaaaat
     2581 atcgttcaat taattcagta tcttcttcac tgactgaaga atcccaagaa gaaaatggat
     2641 tgtcttctaa gatagataga tattctcttg tctgccaagc attttcgaat atacattcca
     2701 catataaagg ctttttccaa ttcaaacgga tttcatgaaa aatctggtct gtgtccgtaa
     2761 attcttttcc ctgcaaaaac aatttcatgg gttccccttc aacagataga tcactgatct
     2821 gcagtcctcg atccgcaaaa ttcgcccctt caacaaagtg gacattgctt aaaatcgctt
     2881 catgattggc aagataattc aagatccaaa aaacttctct tcggctaaaa gatacatgat
     2941 tgaccatcca cgttaaaaaa ttcttctttt cccgtacatc aattagcatt tttctcacct
     3001 ctctgttagg tcatctaata atgacagtgc ctccatatct cctggttcaa gtgttaaata
     3061 ataagtcaat aattcttgcg ctcttttcag ctgcccttct tctcttaaga aaaaggcata
     3121 ctctttcaag aaatctggtt catgtgatag ttcatgataa gcctgttcat agtgtacagc
     3181 tgcaacggta aagtcttcta attcattata agcatgggct aggttccatt ctgcataagg
     3241 attagcagtt tcttccatct gattgatcac ttgtatcaca tcttcatatc tttcatcatc
     3301 caagtaaaga ttacttaaag taagtaatgt ttcatcttgt ttgtctccta cttctaaagc
     3361 tttcagtaaa agttcttctg ctcgctgtct atcatgcaga cggaaagcat tttccgaggc
     3421 caggtgataa agttcgactt ggtaaggatt ttctttgatt ccttcttcta acactgtttg
     3481 tgcttcttca atcatttctt cttcttgaag agcttgtcct agatatagat ataacgattg
     3541 ataatgcgga ttaacttctc gaagttcttc cagatagttg attgcctgct gattttcttt
     3601 caattgaaga ttcgtaaaag ctagctggaa cagtcgatca tctgtccttt cttctttcag
     3661 tgcttcttct aaaggtacga tagctgcttc gaattctcct tgcatactta aggattgtcc
     3721 taaacgttcc tgcatcgaga ttcctgaaat ttcatttatc ccgcttgcca acaatgtttc
     3781 ataaaccact gctgcctctt tgaaacgatc aactgaaaaa tacaattcag ctagcgcaaa
     3841 ctggatcaat ggttcatcag gaagcaaccc agctgcttct ttcaattttg cttcactcac
     3901 ttcagggatt ccgataactt ggtacaaatc agctatcgcc aataagcttt gcggatagta
     3961 atcactttgc ttatccactt tttccaggta ttcaaaagct aagtcgatct cattatcttc
     4021 gattgcaatt tctgctaacg ggatgttcag tccgtcattt tctgggtatt ggttgatcag
     4081 ttgttcaaaa atctgttttg cttcgatcaa aaagccaata gccagcaatt cttcgcctag
     4141 ttcagctaat atatctgctg ggtcttcttt taatgctttt tctaaaaaga tttgtgcttg
     4201 tgacaagtct tcatctgata aagcttgcaa cattttttcg ctatttgtat tcatttcaat
     4261 tccctcttat ctcactgacg atttgttgaa ataccgtcat tgttctcaat aattcttttt
     4321 cgtttggatg ttggtatccg tctattatac ctattttttc taaaacaagt atttttttgc
     4381 ttttttctgc caaaaaaaga actttttcca aatactccat ctttgaaatc tgttccagcc
     4441 agtggattgg atagccaagc aagtccagcc acacaaaaaa gttttttagt tggaaatcaa
     4501 aaggatatgc ttggagattc cacacgaaat gaaacatcat tcctaaaaat tttttcatac
     4561 tagcagaaag tagatggcca ttttcggtca gataaaaagc ttgttcaaac agtttaccat
     4621 aatttgacag gttttctgcc tctaattgat aaaaatagat catttcttcg atcatcccgg
     4681 caaacggccg agtcgttaat tgctttttat ttggatagtt tttatacaga ttttgcaaaa
     4741 aacgatgatc acaaatcatt ccgcaacaga taaaagtcaa aaagtcaatc tgctttccgt
     4801 ccacctgccg ctcgctgatt gtttgatcat aaacgatgcg ttctggcaga ttgatggttt
     4861 gcagcactac acgatttggt agctgcaaaa tcgtccgctg cggtgttaat gctcggctta
     4921 gagaacggat cgatacaggg atcatccata gtgatgaatc aagggttgta tgtttttgaa
     4981 aaaaacctgc cagatccatg atcccttcat ttccaaatcc gatgataaac atggattgat
     5041 tttcgggata gcgtttgacg aaatcaagca ggttctttaa ttctgttaaa tgattgcatt
     5101 gagtgttggc acaaatatac cagtcattgt ttgcttgatt tgcaaataac gggttgatct
     5161 tatctgcaaa caaatcataa tagcgctgat tcgttaaaaa taagattggc tgatcaatgg
     5221 aaagttctct tagctgactg actagcgttt ctccgtaaaa cactttcgtc aacgcatctt
     5281 ctacttgata atcaagttcc atacgcatcc ctcctcttct tatttttatt ttaccattag
     5341 taagcaaatt ctagaaaaga taagagcaaa gctcacctca taacttggaa ataa
//
LOCUS       DACEXQ010000176         5287 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.176, whole genome shotgun sequence.
ACCESSION   DACEXQ010000176 DACEXQ010000000
VERSION     DACEXQ010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.176"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(709..909)
                     /locus_tag="IXH04_002219"
     CDS             complement(709..909)
                     /locus_tag="IXH04_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ1852791.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(1043..1651)
                     /locus_tag="IXH04_002220"
     CDS             complement(1043..1651)
                     /locus_tag="IXH04_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAQ1852792.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNAAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(1736..2206)
                     /locus_tag="IXH04_002221"
     CDS             complement(1736..2206)
                     /locus_tag="IXH04_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAQ1852793.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(2254..3168)
                     /locus_tag="IXH04_002222"
     CDS             complement(2254..3168)
                     /locus_tag="IXH04_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ1852794.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKNVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            3314..4117
                     /locus_tag="IXH04_002223"
     CDS             3314..4117
                     /locus_tag="IXH04_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAQ1852795.1"
                     /translation="MTTKQLIISSFIRFKDLKETRNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKELR
                     SFLSKASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            4295..5242
                     /locus_tag="IXH04_002224"
     CDS             4295..5242
                     /locus_tag="IXH04_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ1852796.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
BASE COUNT         1711 a         1033 c          798 g         1745 t
ORIGIN      
        1 tgttcaatca gcaaattcac tttttaatca taagaaaact ttaaaaaaca tttttgtgta
       61 acgattacta ttttttaaaa tttctcaaaa ttcttaacta tcataccata ccgtgaaaaa
      121 gatcgaaaga ccgaatacac gcagttcgca atcgttttat ctttttttac gtattttttc
      181 cagaaaaagc gaacaacaag gtattttcca aataatcgtg cagtaaaatg atcgcacata
      241 cctttcccat ctttttaaca gtctttgcct accgttcaga ttatcattcg atttcacgaa
      301 aatttcttcc tatgactggt ccttttgtta aacccatctt ctattcgttt tatttccagc
      361 tcactgcatg gctcttaaaa tgactcgaac tagtgatttc attaccatca tcttttaatg
      421 gatagatatg ctcctcttca aagtaaaacg attttctttt aaagtaatag aattgacatg
      481 gtaatgagta tgtatactag tggtttcaca aatggtcttt tattcaacat ccaaaaactg
      541 aaatttcttt aaaatccatt actaacgaag aaaatgttcc ttgcttgata attagttagg
      601 acatgtcaag tgaacaaatg catttactta gccaaaaaat ctaaaaactt cataaataga
      661 caaaaagaca cgactctcta agaagtcgtg tctttattca acaaattatt ataatttagt
      721 cacttcagca gcttgaggtc cgcgtgcacc ttcaacaatc gtgaattcta cttcttgacc
      781 ttcttctagg ctcttgaagc cgtctccttg aattgctgag aaatgaacaa atacgtcatc
      841 tccgccgtct actgtaataa acccaaatcc tttttcattg ttgaaccatt taaccgttcc
      901 gtgttccata aatgtatagc ctccaaaaaa gttcttgcaa gtaaccttgc aagaacatta
      961 tacccaattt agaaaacgct agcaagcttt cgctctttta ttttccaaat tatttttaag
     1021 actgatatga cggaggaaat ggttattttg ctgcttcgaa acgtttgttt acttcatccc
     1081 aattaataac attccagaat gctgcaatat aatctggtcg gacattttta tatttcaaat
     1141 aataagcatg ttcccaaacg tccaaaccta gaattggtgt ctttccttcc atcaatggag
     1201 aatcttgatt tgcagtagag gtaatagcta attttccatt ttccattaca agccaagccc
     1261 atccagaacc aaatcgtcca gcggctgctt ttttgaattc ttctttaaaa gaagaaaaat
     1321 caccaaaagc ttcattaatc gcttctttta tttctcctgt aggttcgcca ccagcatttg
     1381 gtgccatgat ttcccagaaa aatgaatggt tagcatgtcc gccaccattg ttacgtacag
     1441 ctgtcttgat atctgttgga atagcgtcca tatcagataa taattcttct attgtttttt
     1501 cgcctaattc tgggtatttc tcaattgctg catttaaatt cgtcacataa gtattgtgat
     1561 gcttatcatg atgcagatgc atcgtttctt cgtcaatata aggttccaat gcgtcatatg
     1621 cataaggtag atctggtaat gtgtaagtca taaaaaaatt cctcctcaat gttttcttta
     1681 taatgtctac attacaaaga gtaacaaaga gaaggaaatt cttcaattgt tatgcctagt
     1741 cgtctttttc actttgacct acttcttctt caaaaacctc agtttcttta aaaataaaca
     1801 acttgctgaa aatatagttc gctaggataa caataatttg agtgatgatt ttcgccacat
     1861 actcattgct gttcataaca ttgatcaaca ataccattgc ccccatgtcc aatccgtaag
     1921 aaaggatacg gtagaagaag aacttaccgc attcaataag tgcatgtgta aaattcggtg
     1981 attttgaatg gaatacccaa agtttgttcg ttacaaaagc aaacaataca gaacaaaacc
     2041 acgaaataga attggataag gccatactta aatcaaaata ttggcgtgcg agagcaaaac
     2101 taacaaaatt tacaatcgta gccaaaccac caaaaaagag atagactagt atctcccaat
     2161 atccttttgc ttctaaatat ccttttaact gtcgatactg cttcatgagc caacctacct
     2221 tattaaaaat tcttatttat catactctat atttcaataa ctttcatctt tatttaaaaa
     2281 aattaatttt ttttgatcct ttgcctttgt ctctgcttgg atcgtcacat gtgtaatacc
     2341 atattttaat tttaataaag aaattatacg aggatatatc ttttcaatct cgcttagtag
     2401 ctgatcttca aaatcaatat gtccgtcgaa aataattatc ttttcgctat actgccaaat
     2461 atggacatga tgaacattct ttaccccatc gattgcttca atttcttgtt ccattttttc
     2521 atagtcaata gcaggtgcac tttgtatcaa aatagcggca gcttttttga tcaccattat
     2581 cgcttctatc aatatatata tcgaaatgat cagtgtaata actggatcga tccagtagat
     2641 atcgaaaaaa cggatcaaaa ttgctccgat aatcacactt accgaagaca gtgtatcact
     2701 taacaaatgc aaataactag attttatatt caaactttca tctgcttctt gtcgtaataa
     2761 taatactgag aacaaattcg cgagcaatcc aataactgct actgttagca tcaaatgact
     2821 atttatcttt tctggtgaag tgaaccgctt gaatgcttca acggttaaaa acaaggataa
     2881 tatgatcaat atgaatgagt tgatgaaagc actcaatatt tctaaacgct ggtagccata
     2941 cgtacgtttt ttatttgctt cttttcgggc cattttttgt gccccgtaag aaaaaataat
     3001 tgccaatgta tcactaaggt tatggaaagc atctgaaaca agagacaaac tccctgacat
     3061 aatccctcca ataaattctg ataaagtgat caccgcattc aaaaccacta ctccaaatag
     3121 tttttttgat gatctctgtg gctgtgtagg cactattcct tcctccattt tatttatatt
     3181 ggatagtctt tagacaagaa taacattttt ttgatatttc accaatcatt cagatgtttc
     3241 cctaaagcac ttgtatttca cttgatactt cgctagaatt gcaagtgatg acactactag
     3301 atgaggagta cttatgacaa caaaacaatt aattatcagt tcctttatac gattcaaaga
     3361 cctaaaggag acgagaaatg tcccattttg gaaagccatt gtctatcttt tagttttgag
     3421 tataattatg gctttgccaa tctcttttca agtttttcaa gtcttaaaca acattcaatt
     3481 ggatgggcaa aagattgcta ctcgcattcc tgattttaca atccaaaacg ggcagcttca
     3541 aaccgaagaa aaagaaggat tcatttacca aacaaactcc attatcttca catttgatcc
     3601 agagggaaaa cgaacagaac aggacatctc tactgatttg atggggaatt ttgttagtgt
     3661 cgggatgtta aaaaacaaac ttatcatagc tcttcctaat acaggcacga cttctgcttt
     3721 gttgaacaat aaccaattag aacttcccta caccaatgaa tctttaaaaa acttaacagg
     3781 taaggagctc cgttcttttt taagtaaagc atctatacca ctttggataa aagtcctgac
     3841 atttatattt tctatttatc ctagttttct gaatctgctg atcactttgc tatttaccaa
     3901 cattgctgct tttctctatg cacgctttcg actaacaaaa gctacttttc ttgattgttt
     3961 aaaaacattg atctattcaa tttcattgcc tactataatt gcaacaatct tgatggtctt
     4021 tcttccttct tttgacacaa gcgccttcat tgcttttgca ggaatcttta tttttgctca
     4081 agctgtcaaa ggatgggcaa aaatatcgat caattaagaa ataaagacaa ctcgttattt
     4141 ctacttcaat agaccctttt agaggttgtg acattttttt gtcacagccc cttttttatt
     4201 tgaaaaatat ttctttctca aaaaacaagc aaggcgcttc aaagttcttc ttgtatgcgc
     4261 tacacttaga gtaaagaaag gcgggaaatt aactatgtca gaaagaatca tttatgcaga
     4321 tcgtccattt cgtgatgaat ttatcgaaga aatccatact atagcgcctg gttatatctt
     4381 taaaacagaa ttacaaccag aggatctaca aaatgtagag atcagtttgg gctggaactc
     4441 agactaccaa gaaaaactat tagcctcttc taccttgaaa tgggtccaat ctatctcagc
     4501 tggtgttgat cagctacccc ttgagacttt ttcaaagaaa catattttac tttcaaatgg
     4561 tagtggcatc cacagtgaat cgattacgga gcatgtctta ggaatcatcc ttgggtattc
     4621 tcgtggtctt tttcaagcac aaaaagcaca gagtgacatg aaatggttag gttcatcggt
     4681 ccattaccaa tctatccaag gaaaaaaaat actaatcatt ggaactggcc atattggaaa
     4741 aatgattgca aaaaaatgca aagcattaga catgaactgc tatggaatca atacatccgg
     4801 tcatccagca gaaggaatgg aaaagacttt ttctttaaat caactaaagg aagttcttcc
     4861 tgaagtagat ttcgtagtca atatcttacc tctaacggag gaaacaactg ggctctttga
     4921 tcagcatatg tttgaacact ttgatccaaa atctgtattc atcaatgttg gccgtggtgc
     4981 ttctgttaag acacaagatc tggtgcaggc tttaaataac cagcaacttt cctttgcagc
     5041 attagacgtt tttgaggaag agcctcttcc acaagatcat cctctttgga aaatggacaa
     5101 tgtactgatt acttcccaca ttgcaggttt gacacctgat tttcaaaaga aattaatggc
     5161 tattttttta accaatctga aaagctactt tgccacccat gagcttcaaa ccaatcaagt
     5221 aaaattgtct gcaggatatt aatagctgaa aacaatattt tcaaaagaac aaagagaagg
     5281 tgacaaa
//
LOCUS       DACEXQ010000177         5277 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.177, whole genome shotgun sequence.
ACCESSION   DACEXQ010000177 DACEXQ010000000
VERSION     DACEXQ010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5277
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.177"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(199..2838)
                     /locus_tag="IXH04_002225"
     CDS             complement(199..2838)
                     /locus_tag="IXH04_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ1852797.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(3457..4488)
                     /gene="queA"
                     /locus_tag="IXH04_002226"
     CDS             complement(3457..4488)
                     /gene="queA"
                     /locus_tag="IXH04_002226"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAQ1852798.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            4681..>5277
                     /locus_tag="IXH04_002227"
     CDS             4681..>5277
                     /locus_tag="IXH04_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1852799.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEA"
BASE COUNT         1502 a         1171 c          992 g         1612 t
ORIGIN      
        1 attttatgaa caattgccaa aagatattta aaagagagtg tgacttgtga ccgagggggt
       61 ggcaggagaa caccgtttat cgagaggcag taaaagaaac attcagccca ttagcaaaaa
      121 ggaggtgtga caacattcat gtcacacctc ctttttgtat agttccagta aaaagaaaat
      181 caacttagaa acagttaatc attcctcttc ttctgcttcc ggtaatcgat ggaacttgat
      241 cagttcattc accgcgattg gtacgataga taaaatccct accaagaacc agtgattcaa
      301 actgatgtgt actaggccga atagttcttg cgtaaatggc agcaaagcaa cagccgttgt
      361 aatagctaat gccagtaacg ccatttctac caaagcttta ttggtcgcta aattgacttt
      421 gaagatcgat tggctgctgc gtacattgaa cacatgcaga atcgatgaat aagctaagat
      481 caagaatgca acagtttgac cgacatgctg acttgcgtct acccatgggc tgatagtatc
      541 gacataacta cctaaataat aaccaaacaa ggttacgatg gtgaagaccc ctgcatttat
      601 ggcaattttt tgcaataaac ctcgagcaaa gatcccttca tttttcggaa tgggtggctg
      661 atccatgatg ccgtgttccg ctggctctct tcctaaagca aatcctggaa gtccatcagc
      721 aactacattg acaaacagta gttgcacagc tgtaaatggt gctccccatc ctaacagcat
      781 cgcaatcaaa acaataaaga tctccgaaat attacagctt agcaaaaagt tgatagcttt
      841 acgaatattc tgataaaccg aacgtccttg actgacagcg tctacgatcg tcgcaaagtt
      901 gtcatccgtc aaaatcatat ctgcggcact ttgcgccaca tctgttcctg tgatccccat
      961 ggcacaccca acgttacttg ctttcaacgc tggtgcatca ttcacaccat ccccagtcat
     1021 cgctacgact gcacccgtac gttgccaaga tttgacgatc cggatcttgt cttctggcgt
     1081 tacccgagca tagacagaga gagctttgac acgcgcatct aattcttcgt ctgataattg
     1141 atgcagttct gatcctgaaa gtgcttctga cggatcattc aagataccta attctttcgc
     1201 tatggcactg gcagtcacca catgatctcc agtgatcatg actgttttga tgccagcctt
     1261 tttcgctcgc ttgattgctc ctttactttc tggacgtggt ggatcgatca ttccgatcag
     1321 accaatcaaa cgtaagtctt tttccagtgc ttcagaagaa atatcttgcg gttcttcgtc
     1381 ataagtagca taaccaacag caatcactct cagtgcccgt ttaccaaagc gatcattaac
     1441 gatcgccgct tgttctacat cgcctgaact aaagcgcgga gcaagaacat cgaatgctcc
     1501 tttggtgatt gagatgtatt tttcgcccat ttggtgaact gttgtcatca tttttcgttc
     1561 agaatcaaaa ggaagctcgt ttactcgtgg atatttttct tctaattctg cctttgtatg
     1621 ataattctct tcaacagccc ttacgatggc tacttctgtt ggattacctt tgataacaag
     1681 gtcgtcatct tcttgctcga ccgtcacatc tgtacatagt gcagccattt tcaatatttc
     1741 catcgcgctg ttcgtcattg aatcttcgat attcgttact tcatcttctt tgctccatac
     1801 ccggcggacg cgcatttcat tttgtgtcag cgttcctgtt ttgtctgagc agataacatt
     1861 agcggttcct agcgtttcaa ctgctggcaa ctgtcggatg atcgcatgtt tcctcgccat
     1921 cttctgcaca ccgtatgcaa gtgtcaacgt aacaataaca gtcaaggttt ccggtacagc
     1981 tgcaacagcc aaggaaatcg aagtcatgaa catctcaagg agtggttctc cttgcagttc
     2041 accaatcaca aatactaaag ccgcagccgc taaagcgatc atactgattc gtttgcctag
     2101 ctgattcaat cggacctgta aaggtgtttt ttgcatagta ttttcgttca aaagatccgc
     2161 gatcttaccc atttctgtag ccatgccaat ggcagtgacg atcgctttcc cacgacctgc
     2221 agcaaccgta catcctttaa ataccatatt aagttggtca cctaatgact catcttcttc
     2281 agcaataaat gctgcatctt tttcaactgc ttcactttct ccagtcaacg ccgattcttc
     2341 tacacgtaat tgtgatgctt cgatcaaacg ggcatcggca gaaatagcat caccggactc
     2401 taaaacaaga atatcaccca ctaccagttg ctctgactca acagtggtca ctttgccgtc
     2461 tcgtatgaca gttgccgttt gtttattcat atcttgcaag gattccagtg ctttttctgc
     2521 gtttccttct tggacaatcg ccaataccga gttgatcaca acgatagcga tgatcagcag
     2581 tccttcaaat aaatccccat gttctgtagc aaatgcggta taaaaggaaa tcaccgctgc
     2641 tacaagaagg atgatcgttg taaaatcgga caagcttcga aagaattttt tgatcatcga
     2701 ttctttcggc gcctcatcga atttattcaa tccctgcttt tttcgccgtt catccacttc
     2761 ttgtgaagac aacccagtat ctggatctgc ttctgttacc ttctttactt cttctattgt
     2821 ctgcttgtat tcttccatcc tattcctcct ttaaaatctt cgtacaaaaa aaggtactta
     2881 ttttactata acatcctttt tttatttact tcaaataaag ggcttatcga ttatttgtta
     2941 agaaagcgta aaatttaaag tgcttatatt tgcaaacgct accaaaggat gctattatat
     3001 agatgtaagg aaggaggata ccatcaaata gacagaagaa tttctcgata gaacagtgta
     3061 agcaaacaaa gttgttagaa gtacaggttt cacccagaca tgcgtgtaga gtacaaacct
     3121 ataaatagtg aatgcgtatg gatgtttgga tcacgaagta tccgaaacaa gaagtaatgc
     3181 gatacgtaac aattgaataa cgtaaacgtc tgaaaattga ataggaacaa gagatgtttc
     3241 aaatgttgaa gatgtaacga aaacaaaatt tttgtttaac atgaagaaat tcaacaccgt
     3301 gctttgcatg tatgatggaa agttacaatc atttctgttt atttgaatcc ttccgaggta
     3361 caagaaagcc gagcttatcg agagctcggc tttttctttg tcataaaatc tttgtaaagc
     3421 ttttttgtta ttcaaaacta gcggcttttc ataaccttat ttcacgaaca tcgcatctcc
     3481 aaaactaaag aaacgatagc gctcttcaat cgcatgctgg taagcagata aagtcaattc
     3541 tcttcctgca aaggcactaa ctaacatgac taaagtcgat tttggcaaat ggaaattagt
     3601 tgagaaagct tctaccactt tgaattcata gccaggcgta atgaaaatat cagtccatcc
     3661 gctgtctgct ttgatttctc cattaaattt cgttccaatc gtttctaatg ttcgaattga
     3721 agttgttccg acagccacga ttttcccgcc atttttacgt acttggttca atcgctcagc
     3781 cgcttcttcc gtcaaacggt agaattcact atgcatatgg tgttcttcaa ttttatctac
     3841 gctgacaggt cggaatgtac ctaaccctac atgtagtgtc agatagacta attcaacacc
     3901 ctttgcttcg atctcatcaa gtaattcttt cgtaaaatgc aatccagctg ttggcgctgc
     3961 agcagaacca ttttctttcg catagacagt ttgatagcga tctgggtctt ccagacgttc
     4021 tttgatatat ggaggcaatg gcatttctcc caatgattca agaatctcta gaaaaattcc
     4081 ttcatactca aagtcaatga tccgtccgcc gtgttccaat tcttctttca ctactgcttg
     4141 caatctgcca tcaccaaaag agattcttgt cccaactttg gcacgtttcg caggtttgat
     4201 caatgtctcc cacgtatctc cttgtgtatt cgttagaaga aggacttcta aatgcgcacc
     4261 agtatctggt ttttctccat ataaacgagc aggcagaact cgtgtattat tcataactag
     4321 cgcatcacca ggctgtaact cttcaatgat ttcatggaaa tgtttatctt ccatctctcc
     4381 agttttccga tccaaaacaa gtaaacgaga atgatcgcgg tcttttaatg gtgtttgtgc
     4441 gattaattct tccggcaagt caaaatcaaa atcttctgtt gttaacatat cgtcactcac
     4501 tttctatcat gtgcttcatc attctatcat gtttttcgtt ctgttggcaa aatcagcact
     4561 ttttcacttc tattttttgt tttcctaaat aaaaaaacta ccctatttgt attttttcat
     4621 tgttttaggc atactagtaa tagatagttc ttcattgaaa atagaaaaag aggtggcacc
     4681 atgattgaat ttcaaaatgt gtcaaaaatt tataaaggtg gaaaagtcgc tgtagaggac
     4741 gtcaatcttt catttgaaaa gggtgagttc atttgtttta tcggtacgag cggaagcgga
     4801 aaaacgacga cgatgcggat gatcaaccga atgactgatc caacacaagg gaaaattttg
     4861 attgatggaa aatccatcca agaaattgat ccagtggaat tgcgacgtca gatcggctat
     4921 gttatccaaa gtattggatt gatgcctcac atgacgatcc gtgaaaatat cactcttgtc
     4981 caaaaattgt tgaaagtcag tcaagaagaa cggaataaaa cagctgaaaa aatgattgat
     5041 cttgtggaat tgccccgtga gatgttagat cgctacccac atgaattatc tggtggtcag
     5101 cagcagcgta ttggagtcgt tcgtgccctt gcagcgaatc aagatatcat cttgatggac
     5161 gagccattcg gtgcgcttga tccaatcact cgtgattcct tgcaagacct tgtcaaagat
     5221 ttacaagagc gtttgggaaa aacgatcgta tttgtaaccc atgacatgga tgaagcg
//
LOCUS       DACEXQ010000178         5243 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.178, whole genome shotgun sequence.
ACCESSION   DACEXQ010000178 DACEXQ010000000
VERSION     DACEXQ010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5243
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.178"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(161..1072)
                     /locus_tag="IXH04_002228"
     CDS             complement(161..1072)
                     /locus_tag="IXH04_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1852800.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(1475..2053)
                     /locus_tag="IXH04_002229"
     CDS             complement(1475..2053)
                     /locus_tag="IXH04_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1852801.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(2073..2741)
                     /locus_tag="IXH04_002230"
     CDS             complement(2073..2741)
                     /locus_tag="IXH04_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1852802.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(2756..3826)
                     /locus_tag="IXH04_002231"
     CDS             complement(2756..3826)
                     /locus_tag="IXH04_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1852803.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            complement(3997..>5243)
                     /locus_tag="IXH04_002232"
     CDS             complement(3997..>5243)
                     /locus_tag="IXH04_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1852804.1"
                     /translation="YVSNGINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELD
                     PDKHQLILASKEGKNETITYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAA
                     LKEAKEVVIFGAGYIGLELADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQT
                     KQINFYPNEFLIDWKKSEEKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYED
                     ETVIVNEYLQTSDPDIYAIGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPT
                     AYDLRQKITAAVIIDYFLGTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAK
                     LIYHPDTLEIIGGQLISQEFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHY
                     LNELAFKVLAKRANSNRVDKRL"
BASE COUNT         1486 a         1084 c          850 g         1823 t
ORIGIN      
        1 catgagaagc tgttttttga aattgctcct tgtgacgcgg agttaaacgg caggctcaca
       61 acctcttcct taaacggtgt tcaaaagctt gcatctgcga taataagccc ggccaaacga
      121 aaaatatgag gagctatttt tgtttggctg ggcttattta ttactgaaaa taagtggtca
      181 atgcttgtgt caaacttgca gcaatctgct gctgataact gtctgttact aattccttta
      241 aatcactatc attattcata taacccatct cgattaaaat agaaggttga agattttctc
      301 gaagaacttg atagttccca gtggagattc ctcttgaatc cagaggcaga tcagtgaagg
      361 agtcagcaat tgtctgtgct aatgtttcct cttgtccata gtaataatat gtcgtgattc
      421 cagttgcttc attcgcatat tccgtagagt ctgcgtgtaa actgataaag acatctgcct
      481 ttttttcttc actgtagtcg catatatctc caagctgtac taattcatct gtagtatggg
      541 tcaaataaac cgttgctcca gcatcctcta gcgccttttt tacttttatg gctgtactta
      601 atgttatatc tttttcttct gtttgttcat cattgcttaa tgccccagta tcatttccgc
      661 catgcccagg atctaataca atcactgcat cagatagctc ttccggtttt gtttgtatat
      721 tctttgtgac tttggttcca tcttcgaggg ctaaatatcc agtatagcca tcatttgtct
      781 tgatctgata agcatttttt gtttcagaaa gaaactccgc cagctctccg ccgtttatag
      841 ttgcaagtgg ggtagttagc gagtcatctg tatatagtgt acggtcattt tttccgattt
      901 tgattaatgt ctcttgttct gtatcatcaa atgattcctt tgtctttgta accgtactgt
      961 tcgtactgct gaccgtttgt ccaactatct ttttttgacc aaaaacatct atttgaaaac
     1021 agataataat gataatgatt gcaaatacaa aagctccaat tatttttttc attcttgact
     1081 ctcacctcgt agatccagac taagggtcaa aacttaaaat cagcttaagt acgcaaaaaa
     1141 ggggctgtaa aagatataac cgtagaaaat aacattactg ctaaaatgat ttttttcatt
     1201 tttggcatat cctgaaatct ccttttgact attcgtggtg attcatctac ccaataaata
     1261 gtttccttca ttctacaaac aattaatttc ttcgttatta aattaaaaca cttcctggaa
     1321 caccgacata ataaataaaa atataccctg ataatcatga gagaataaca aatttgtatt
     1381 tatgaaaaac tacctatata aaaaaaacaa gatataacga tcttcgtcat atcttgcttc
     1441 ttcacctaac tatttttctc cctatataac agctttacgc ttctcctaat acaccattca
     1501 aatataaatc gatcaattgc gccacattta gcggttcttc cacaggtctt ttcgaccgag
     1561 tgatgtaagg tgctaacact aagaaaaaat gccttgctgc gatttctttt gaaatcccat
     1621 ttcttagcac agattggttt gctgaaaaga tttcttcaaa aggttcacga taatttttct
     1681 tccatagaaa gaaaagatga tctcttgttt cctttgaaag attgctttca agatcgtgaa
     1741 gcatcagaaa caaatcgacc agatgtgtct caatcaaaaa ctgcgccatt tttgtcaagg
     1801 ttacttgaaa tcccgtttcg cttgtttgaa cgatttctct caaagctttt ccaacatctt
     1861 ttaagtggtt ttctaaaact gccgtatata gtttttcttt atcaccgaaa taatgataga
     1921 gattgggttg ggtgatccca actttttgtg cgatctctct tgttgaagtg ccgagatatc
     1981 ccttggacat aaacaagtca gtcgcttctt tcaaaatttt ttgctgtgta tctttgttgt
     2041 atcgatggac catttttcca cctgcttttt ggttattctt tttctttcaa tacaccatct
     2101 tccatcataa atattctatc gcatttgtca atcagccgtg tatcgtgagt aaccatgatc
     2161 gttgctttat ttttgtcttt tgattctttt gccaagatat cgactacttc tcgtgccttt
     2221 tctgaatcca aactagcagt tggttcatct gctagaataa tggacggatc attgtacaat
     2281 gcacgagcga ttgcgaccct ttgccgttct ccaccagata gttcttctgg atacttgttg
     2341 atcaacttat ccacgccaag ctgctcaaat aattcttttg cttctttttg acgatgttca
     2401 tgtttgatct tatctactaa taataactgc ttttttactg tcagaaaagg aaccagattg
     2461 gaagcttgca aaataaagcc aatttcttta aaacgtagtt taacgcgttc actttctttt
     2521 tgattactga attcttgatc attgataaat acagagccac tgctaggcgt ctgcaatccg
     2581 cctgccaaag tcaaaaaagt acttttacct gaaccagaag gaccgatgat tgcaacaaat
     2641 tcccctttat cgatactcat ggttgtttct ttcaatgctt taactacttg gtcgccatct
     2701 tggaaacttt tttctacttc tttaaattct attgctttca tgctcttcct ccttcttatc
     2761 caattgcctt caacggatcg atttttacga ttgtccgaac agaaaatagt gcgcctaaca
     2821 cagctactac cagcatcaac aagctaatga cgccaaaaaa cagccaattg ctttggaatg
     2881 gaaccgcatc aggtagaaca agggatgttc ccactgttcc taatagacca agaagaatac
     2941 caacaaatga taaaatgaat gtttgtgcca ctacagaacg cgcaataaat cctcctgtga
     3001 tcccttgtgc tttcattaca ccaaagatat taatcttttg catcgtcagt acataaatga
     3061 aaatcccgat cacaatagcc gcaataacga tcaaaaatcc gatcataaat ccaaaagtca
     3121 atacttgcgc attatatcca ggtaattcat tgatgaattt agaaattttg atctgttcta
     3181 aatcttcagg taaattactg attttacctc tgacaactat tgcgttaatc cgtgcatttt
     3241 cacttgtatc ttctttttcg aagcggatct cttggtaagc attgatggtt gtatagagaa
     3301 ctggtgagac attgaatttg gcatgatccg taaagccagt tagtttaaac gtcttgtcac
     3361 taccagataa cttaactgta tctcctacgg ctagaccata ttcttctttc aaactaatat
     3421 cgcctactgc ctcatcatcg ttatcaaaag cttttccttc aacaagattg ggcatgatga
     3481 attgattttt atcgataccg aagaagctaa cattgatttt tccttcttct gtactgtctt
     3541 tactagtcac gactccaggt gtctgggcaa gatacgccac tttcccacct tcgacttctt
     3601 ctgctatctt cttagtaatc atggacatcc ctagatttga atttgcatct ttcgaaagca
     3661 caatcgaatc tgcctcccac ttgtcgactg ctgttcgatt atcttgagct aagccataag
     3721 ctaacccagt caaaaaaaat accaggtagg cgatcaaaaa catcacccct gcaactaacg
     3781 cataacgtaa ctttgaatgc ataatttcat ttaatgctaa aaacatttaa ctcctcctcc
     3841 tgaaataatc attttataca acaattttat cacttgataa atttttggcg aatattatgc
     3901 tcaaaaaatt cttccgctat ttcattatat gaatttttta ttctcgattg attaatattt
     3961 attatatccc cacacaataa aagaccgtga tatttctcac agtcttttat ctactctatt
     4021 gctatttgct ctcttagcta gtactttaaa agctaactca ttgaggtagt gaaaacgaga
     4081 ggtgtgttca gacagaaatc caaaatcctc aactgctagc tgtggaatcg tagttttgtg
     4141 tttcacgata tccgcaagca ggttcaaatc tgataataag aattcttggc ttatcagctg
     4201 tccgccaatt atttctaatg tatccggatg gtagatcagc tttgcattta ctgttttgtt
     4261 ttcttcatcg tattgtggaa atagatcaaa ttctcctgaa cacgaaccgg tattttgttc
     4321 caagaatggc gcttcatctt ctgtcagccc tacagttcct aaaaagtagt ctataatgac
     4381 tgctgctgtt atcttttgtc tgaggtcgta agcggttggc tgaccactca aagttagcgc
     4441 tactgctctt gccatatgta cagctcgagt aactaaaggt aaaaagagat gccgtttatt
     4501 tttgtcaaaa ctgactggga cgatatctcc aatcgcatag atgtccggat cacttgtctg
     4561 cagatattca ttgactatca cagtctcatc ctcatacaaa gccacttttt cttttaacaa
     4621 tgtcgtatta ggacgagttt gggcagaaaa aatgaccata tctgccttta ttgcttgaga
     4681 tagaagttgt acggatacta ctttctcttc tgattttttc caatcgatca agaattcatt
     4741 tggataaaaa ttgatttgct tagtttgcag ctgattttga aaactgttga tcatatcctt
     4801 atcgaaatat cttgataaaa cattcggcat ataatcaact agatgaataa tgtatccttt
     4861 atttctcaaa gcatctgcta attccaatcc aatataacca gcaccgaaga tcactacttc
     4921 ttttgcttct tttaaagcgg caagtacttg cttagattgc actagatttt tataggtgta
     4981 gacattttca gcttcaattt gtttatacaa ggtgacagga gaggaacctg ttgctacgat
     5041 taatttatca taggttatgg tctcgttttt tccttctttg gatgcgagga tgagctgatg
     5101 tttgtctgga tcaagttcag ttactctcca ttcggtgatc agttttgccc cactttcttg
     5161 aagagtccgc atgttatggg caacttcagc taaactacgt atctttcctt ttaaataaag
     5221 gttgattcca tttgaaacat agc
//
LOCUS       DACEXQ010000179         5180 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.179, whole genome shotgun sequence.
ACCESSION   DACEXQ010000179 DACEXQ010000000
VERSION     DACEXQ010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5180
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.179"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            488..676
                     /locus_tag="IXH04_002233"
     CDS             488..676
                     /locus_tag="IXH04_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAQ1852805.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            754..1767
                     /locus_tag="IXH04_002234"
     CDS             754..1767
                     /locus_tag="IXH04_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1852806.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(1834..2868)
                     /locus_tag="IXH04_002235"
     CDS             complement(1834..2868)
                     /locus_tag="IXH04_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1852807.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            2983..>5180
                     /locus_tag="IXH04_002236"
     CDS             2983..>5180
                     /locus_tag="IXH04_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAQ1852808.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQT"
BASE COUNT         1632 a          961 c         1065 g         1522 t
ORIGIN      
        1 aatgatcgta aaaacatgag aaaaggccct aaaatcttta ataaatagac gtgctaaaga
       61 aagactaaga agtgttaact tttgttattg tttcttagtc tttttagtta gtctacatta
      121 ttttggcgat ttgccaataa ggactgttga gttgtgcaaa atcaaatctg ggtcagaatg
      181 aacgtcattc tgtctcagat ttttcgttat ttcaatttga ttaactgatt gatcaaaaag
      241 atttttattt tcatattctc aaatgattca acactaaata tacatgtttg acgcggaaat
      301 tattattcga ttttggcggc atcagggggg atgacgacgt agcagaccca ttattttact
      361 caagaaaatg tccgtgccta ttatttagaa tacgatagtg aacgtgctgg gagttttgtt
      421 cttttagaga aagtctctga agataaatta gttgtcttag attttgactg taacgaaaaa
      481 tggcgaaatg gaagagcacc agcaagtgat tgaccggatc tacgaagcga cacgatatct
      541 gcctttggat cgtttgtgct taagtcctca atgtggattt gcttcaacag aagaagaaaa
      601 tgttttgttt gaagaacaat ggaaaaaatt tcttttgtca aaagcattgc agaagaagtt
      661 tggggaaaat cattaaatca tctaggagag cttgatagga aataacacta tggtataatc
      721 agaatgctta atactaacta ctgagggagc cttatgaaaa agaaaattct taaatttatc
      781 ggatggttct tcggaatcat tcttgggatc gcgtttgcaa tctttcttgc cttccagctt
      841 tcccccagac ctggagcatt cttgatcaac catatgtttt caaatactgt tcaaatcact
      901 gataaaaaaa cgttcgatca agcagaaaaa aacgtggcga aaaaaacaga tttgacgtat
      961 gagtccaagt ataaagagaa tacttatgac atctactatc caaaaaacag caaggggcct
     1021 gttcctgttc tattttgggt acatggtgga ggattcgtcg gcggagataa atcgggtgtc
     1081 aaagaatttg cgacaaaatt agtttcagac gccaacatcg cagtggttgc gatgaattat
     1141 gaattagcgc ctgattcaga ataccctaat caagttcttc aagtgaatga actgatcaaa
     1201 gatttattag ctgaagaaaa agacgacaaa ttgctagaca tggaacaatt attttttggt
     1261 ggagatagtg caggttcaca aatcgcattg caatatgcag ctatacaaac gaatgatgcg
     1321 tatgctaaac aaatgaaaat caaacagcta cttccaaaag atagtctgaa aggaaccatt
     1381 tcttattgcg gacctgttga tttgaagcta acagcacagc agcattcgga tgatcgattt
     1441 atgaagtttt tcgtcaaaac cgttgcgtgg tcgttgattg gaacaaaaga ttggaaaacg
     1501 agtgagcaat tgaaagaagc tagtgtggct gaccatgttt ctaaggaatt tccgccaacg
     1561 tatataactg acggaaatgc atactcattc caggagcaag gtcaagcatt agaaaatcgg
     1621 ttgacagaat taaaggtacc cgtgcaatcc ttgttttata cagatacaaa aaaagagatt
     1681 acccatgaat accaatttga ttacacgcta aaagaatcgc aaaattgtta ccagcagaca
     1741 ttggattttg tgaataagta taagtaagag gttgtgaaaa agccgtttcg ttttgagtat
     1801 gaagaaacga agcggctttt cacaacctct ctttcaatac ctttttgttc ttgggataaa
     1861 actttcccta acaatcaatc gtgcaggata agtcaaaatg attggttcgt cccgtatacc
     1921 gtcaattcgc tccaatgcca ttcgaacagc tgcttgccca attgatttcg cattgatttg
     1981 aacactggtc aagccagggg agaggtttgc gattgtctcg atattatcaa agcttgctac
     2041 aacaatctct tctccaattt ggatattttt ttgtttcaat gcccgataga caccaattgc
     2101 cattggatcg ctcccgacaa gaatggcagt aggaagaggg gattcatttt ctaatagatt
     2161 agtcgcaagt acctcacctt gtttttctgt ccagccacct agatgataag acatatattt
     2221 ttctaagtga tgatcgatca taaaatgctg ataggcacgc atcctctttt catttataga
     2281 aatctgcttc ccaccgaatt catccatatc gactttatat cccccgatat aggctattct
     2341 tttatgacca ttttctaaga acaattccag tacggtttta gtcatccgtt caagatctgc
     2401 atacaccata tcgacctctt gctggatgtc agggttatcc acgacgacta tttgcatgtt
     2461 ttgttttaac aaggcggtaa tcgattcttt cgtaacagtc ccaacaacga tcaccgcacc
     2521 taactgatct agatcttccc attgtttagg attatcagat agattataga ttcgattcat
     2581 ccctaaatgc agacgtcgag cttcttcttc catccctact cgcaaatgtt tgtaatagat
     2641 atcctgttct tcgctcgctt tgccgatcgt agagatcaca ccaatcgaag gcattctctt
     2701 tttgatatat ttacgagtac tgatgtcata atccaacgca gcagctgcct ctactatccg
     2761 ttgccgagta gtctgagcca catggatcgt ctgatcgtta ttcaacacac gggaaactgt
     2821 agcaggagag acatttgcct ttcgtgcgac atctctaatt ccaaccattt ttacacactc
     2881 ctcaaaaaaa taaaaaattt ttattgacaa aattgtttag taaacagtat acaggaatta
     2941 taaaaaaaag taaacggttt ccgttttgtt gggagtgaaa tgatggatat tacgcataga
     3001 aaaacagata aaaatttcat cttatttgat gaaaagagcc gagtcttcca tttacgaaat
     3061 acatttcttt cttatctgat aaagatagaa gaaagcaatg tattggcgca tatttatttt
     3121 ggcaaaccag tcaagcagta caaagacaat aaaaattatc cccgaagaga tcgtggattt
     3181 tcaggaaatg ttcccttaaa tcctgaccgg tctctgtcga aagatacact accgcaagaa
     3241 tactcttctc atggcagcat ggactttcga acgcccgcat cgatcattca gcgcaagaat
     3301 ggatcagact tactggatct gagatatgac agccattata ttacagatgg gaagccagag
     3361 atcgaaggct tgcctcaaac ttatgtgatg gacaagtcag aggcacaaac gttggtgatt
     3421 tcacttaagg atagagaaac agcgatttat ttcgatttgt tctataccat atttactgat
     3481 agagcagtga tcacacgatc tgtaaagata agaaatgaaa ccggagaaac aatcaaacta
     3541 gaaaaagcgg ctagtttcca gctagacttt gcccatacta ggcgatttga tgaagtcatt
     3601 gctttgccag gagcacatgt caatgaaaga cagatttcac ggcaatctgt tttatctgga
     3661 acaaaagtat ttgaaagccg tcgaggaacg agcagtcacc atatgaataa ctttattgct
     3721 ttagttcatc atcatacgac agaaaatact ggagaagcga tcggacttca atttgtttat
     3781 tccggcaacc attctttcga acttgaaaaa gatcagatca atcaattgag agtcgtcggt
     3841 gggatcaaca gccatcgttt ttcgtgggaa ttgaatgcag gccagtcttt tcaaacacct
     3901 gaaatgatcc tttcttactc aagtcaggga ttgaacaaaa tgagtcagat ccaccatgag
     3961 ttgctacgtg aaagaatcgc aagaggtcgt catcaatttg ctgaacggcc gattctggtc
     4021 aataactggg aagccactta ctttgacttc aatagtgaaa aaatcaaggc gatcattgat
     4081 gaagcaaagg agctaggcat cgaaatgttc gtattagatg atggctggtt tggaaaaaga
     4141 gatgcggaca attcttcatt aggagactgg ttcgagtatg aaggcaagtt gacaaatgga
     4201 ttacgtgaaa tagcagatta tgctcatagc aaaggattga aatttggttt atggtttgaa
     4261 cctgaaatga tctcggttga ttctgaactg tatcgaacac atcctgattt tcttatgcag
     4321 gagccaggta gaatgccttc tgcttccaga tcacaacatg tactggattt tacaaggtta
     4381 gatgttcggc agacgattga gaaacaaatg agaaagattt tagatacgat tcctcttgac
     4441 tacatcaaat gggatatgaa ccgttcgctt tcagatgtct attcgattac ccttgaccca
     4501 cagagacaag gagaagtagc gcatcgttat atgctcgggc tttatgaact acttgaacat
     4561 ttgacaactg attatccgga aatcttatgg gaaggctgtt ctggtggagg tggacggttt
     4621 gatgccgggt ttatttatta tatgccgcag tcatggacaa gtgataacac ggatgcagtg
     4681 gagcgtatga aaatccaata tggaacaagc cttgcttatc cgatttcgtc catcacagct
     4741 catgtatcag ctgtaccaaa tcatcagacc gacagatcta cttcgttgaa aacaagagga
     4801 gaaacagcta tgagcgctgt ttttggttat gaactggatt tgacaaagct gtctcctgaa
     4861 gaaaaaaagc aagtaaaaga ccaaattata tcctatcaaa cgattcgtcc ggtgatccag
     4921 tatggccatt attatcgttt ggcgagtcct tttgaagaaa atattgccgc atggatgttt
     4981 gtgtctccta aacaagatga agctattgtg tttttaggga ggatactagc ttcagctcaa
     5041 ccagcgttcc atgaagtata tctgatgggg ttagatgatg aggcacttta tcaggaacag
     5101 acctcgaagc ggatattttc gggggccgaa ttgatgacag ttggacttta cttccccgat
     5161 tttcaaggtg atttccaaac
//
LOCUS       DACEXQ010000180         5179 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.180, whole genome shotgun sequence.
ACCESSION   DACEXQ010000180 DACEXQ010000000
VERSION     DACEXQ010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5179
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.180"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(2..799)
                     /gene="recX"
                     /locus_tag="IXH04_002237"
     CDS             complement(2..799)
                     /gene="recX"
                     /locus_tag="IXH04_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAQ1852809.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            906..2288
                     /gene="rlmD"
                     /locus_tag="IXH04_002238"
     CDS             906..2288
                     /gene="rlmD"
                     /locus_tag="IXH04_002238"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ1852810.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(2462..3040)
                     /locus_tag="IXH04_002239"
     CDS             complement(2462..3040)
                     /locus_tag="IXH04_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAQ1852811.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            3362..3832
                     /locus_tag="IXH04_002240"
     CDS             3362..3832
                     /locus_tag="IXH04_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAQ1852812.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGEYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(3940..5166)
                     /locus_tag="IXH04_002241"
     CDS             complement(3940..5166)
                     /locus_tag="IXH04_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAQ1852813.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
BASE COUNT         1534 a         1003 c          891 g         1751 t
ORIGIN      
        1 tttattcact ttcctcttcc ttttccgtaa tgaatcgctg gatcatatcc aaatcaaatc
       61 ctttttgata taagctttgc tttactttta gatttctttt tttaggttca aaccttaaat
      121 ttcttcgcca tagtttatct ccttgtttga ctaaataatc atattctatt tcttgatctg
      181 cttcttgcgg aagatcagct atcgcttgtt ggatcacttc ttgagaaaag cctttcgtca
      241 tcaatgtttg ttggattttt ctcaataatt ctttttggct ttttccgtga ttttttccaa
      301 acgctttttc ggctgtctga tgtgcatttt gaacttggtc ttcaaatgta tattggctga
      361 gcgcttgttc aatgatttca ggtttcaaac ctttttgctg catctgctgc gctaatttct
      421 ttggtccttt gtcacttaag cgcatattcg tgcgcacata actttcccca tagaccaaat
      481 catcgacaag gtccaattct tttaagcggg ctacgatttt atttcgatct tcttgaagga
      541 tttccttttc ttttaagtat gttcggattt ctttttccga tcgcaactga taacttaagt
      601 aattcattgc taggtgaaaa ccgaaatcag ctccgctact cttcttgatc tcttggatag
      661 tttcttcgtc taattcccgt ccttttaata atcgatagcg tactaataca tcttcagata
      721 cttgtaaagc ttctccttca gataaatcta cgcgatagaa agggcctttg cctttactga
      781 ttctcgtaac ggttaacata aacatcctcc tcgaacatat attctctatc ataccacaga
      841 aaatccccgt aatcaggtat tatatgttag aataaaccac agaaaaaaga aaaagggaga
      901 tacttatggc agaaacaatt gtcaaaactg gacaaacggt tcctttgaaa atcaagcgtt
      961 taggcataaa tggtgaagga atcggttatt tcaaacggtt gatcatattt gttccatatg
     1021 cactaccaaa agaagaagta ctagtgaaaa tcacgaaagc aactcctcgc tatgcagaag
     1081 gagaattaat aaaagtcaaa aaaacaagca aagatcgcgt agtggcacca tgtcctgttt
     1141 actatgaatg cggtggctgt caattacaac acttagctta tcaagcgcaa ttggatttta
     1201 aaaaggatct gcttttgcaa gcattggaaa aattcaaacc agcaggcttt cgatcttatc
     1261 aacttttgcc gacgatcggg atggatgagc cttggcatta tagaaacaaa gcacaatttc
     1321 agttacgaaa aaataagcag actcaaaaaa ttgaagctgg tttatacgaa gcaaactctc
     1381 atcagttagt ccctataaca gactgcttgg ttcaagaacc tcgaacacaa aaagtaatga
     1441 atactgttgt tcaactattg aataaatttg atgcacccat ctatgatgaa cggacaaaca
     1501 gtggaatctt ccgcacaatc atggttagaa tcggtgttaa aactggggag ttacaagttg
     1561 tttttattac tcgcagtaaa aaattcccgc aaaaaaacgc aatgatccgt gagatcaatg
     1621 aacgactgcc cgaagttgtt tcaatcatgc aaaatgtcca ggcacaaaaa acgtctctcg
     1681 tgatggggga tgaaacgatc cacttatggg gaaaagaagc cattgaagaa caaatcaatg
     1741 aagtgacttt cgatctttct cctcgtgcct tctttcaact caatcctcag cagaccgaat
     1801 tgctttatgg cgaaggaatc aaagcattag atgtccagcc taatgaaacg atcgtggatg
     1861 cttattgcgg agttgggacg atcggcttaa gtgtagcaaa acaagccaaa gaagtacggg
     1921 gaatggatac cgttcctcaa gcaatcgctg atgcaaaaaa aaatgcggaa agattaggat
     1981 atcacaacac tcgatatgaa gtcgggacag cagagcaagt tctgcctaaa tggttgaatg
     2041 aaggttttca gcctgatggt atcatcgtag atccaccacg tacgggtttg gataacgcat
     2101 tgatccaatc attattgaaa taccctccaa aaaaacttgt ttatatctcc tgtaatgttt
     2161 ctacacttgc cagagatctg gttaatttaa gtaaaaagtt caagattgaa tatttacagt
     2221 ccgtcgacat gtttcctcag actgcacgtt gtgaagttgt cgtgaagatg acgagactcg
     2281 ctgactaaat gaaagaggtt gtgaaaaagt ctgccctgta tcaagtaata agaacagcca
     2341 aacaaaaata gctcttcatg ttttatgaat actgtacttg tgaccgaaga gttaacagga
     2401 ggacactatt tatttaaaaa aagggttgtg acatagtttt tgtcacagcc ctttttgatt
     2461 attattggat attcacgtct atcacttgat aaaatgcatt tgtcgtatcg ctgatattcc
     2521 atactgctaa aatcacatta tatccttttc gatcctgcgg aagtgtgatc gtttggttga
     2581 ctgtttttgg tggaacagtt gctttgtcgt ctatttttgt gatcaattca aaatcactaa
     2641 atttcaaagg ttgattagga ttccaagttg gtttggtaat gtagtaatcc caagtacttg
     2701 tccgatgttg tgctgttaaa ttccaagtaa tatttaatgt tcctggatta accatgtttt
     2761 tatgccatct gttagatgtc tgttcatcta aaggctcaaa gccgctgact cctgcactag
     2821 caatttttcc atcaatgaat gtatttttgg tagcttcgat actttgcggt tcccattgtg
     2881 ctcttccgac attttgattt aagtttcctg ctgctgatgt tcctaggtat gcacggcttc
     2941 ctggagaagt aatataacca tgcgcaaaac ctggaatgct aaaaccgaac aaccctaaac
     3001 taactaaccc aataaacaat aacgtactaa tctttttcat gtagaaacct ccttcatttg
     3061 ataaccacat tgtaaacgct ttattgtatt aacacaaaca ttatgctttt ataattttat
     3121 gttgaagtaa aaaaggtgaa aaaagtaaga tgcagattat aacagaagta gacgagaaaa
     3181 gtacaaatag aaaacatgag aatcaaatca tatgatcttt ttcaaaaaca tcattcatat
     3241 aaacaaatgt tcaacttttt aataacaaca attatcaatt tttttcttac ttcacaaact
     3301 aaatacaggt cttgctgtga gccttcatgg taaactacta ttatagaaag gagttagtaa
     3361 aatgaagatg ataaaagata ttcgtttttg ggtttgtgtt ataggaattg tcatattagg
     3421 ctttttatca gggttgctta gcggaaatcc cggagaatat tactattctt tgcaacttcc
     3481 accatttgca ccacctagct ggatatttgg gccgatgtgg accttactgt atatattgat
     3541 gggtatttct ctttatttgt tacttaatca caacaacaaa aaacagcgaa acaatcttgt
     3601 cggtcttttt gtgattcagt tcatttttaa ttttatatgg tctgctctat tttttaattt
     3661 aagaaatatt ttcattgcag caatcgatat tacactttta gttattttct tgtcagtgct
     3721 tatgtatcag ttgtggctac atcatcgcct ggcaatgtgg ctcatgattc cttattattt
     3781 atgggtacta ttcgcaactc tattgaatta ttcaatttat tttttaaact aagcgctaaa
     3841 aagagacgtc ctgtaattta acacatagaa aaagtgccac ctaagttaga ttttttgtct
     3901 aatttaggtg gcactcttta ttaaccaaac tatttttttc tagtacccac ttgttgttcc
     3961 atactcatta ttctggtcat aaccataacc attttgttcc tgtccagcgt aaggatcata
     4021 actttgagta tcttgacttg gtgtatagta ctgacttgta tcttctgttt gctgataata
     4081 ggtattcgga tcttggtact gatcatatgt gttttctgaa taggaagaat aatcggtgta
     4141 cccttcacta ttcgatccat caccgtttgt cgaatcgcca tcttccgaat catcaaagaa
     4201 catcattgaa gcattctcat ttttcaaatt aggtaatgta tcgcttgttt ttatatttaa
     4261 ttgtttcttc agttcatttt ggatcgaaag aagatcattt tttcctaaaa tttgatagta
     4321 gatatcattg atcatttgtc cttcgccttg caactggtct tgtttgattg tttcaaacgc
     4381 aggggtgtag ttcgtcgcta catcaagcat gtcatcccaa tctaagtccg tcgtcacatt
     4441 gttctcaact gcttttaaaa tttttctata attgcttacg ccatctaaac ttaaaacttt
     4501 attgacgatt ttcgtcacta cttcccgctg acgacgttgt cgaccgatat cgccttctgg
     4561 atcttcgtgt cgcattcgag aataggctaa acctttttct ccattcaatg tttgttcacc
     4621 tactggtaca tggacaccat ccaaagtaaa gtcaatttta ttattcactt tgatgccacc
     4681 gaccgcattg atcaaatctt ccatgccatc caaattgatc gttacataat gatcaatagg
     4741 aatatcgagc agttgctcaa ttgaatccat ggccatttcg acaccaccaa atgcataggc
     4801 atgatttaat ttatctaccg ttccataacc tacgatattc gtataaatat ctcggtccaa
     4861 actgatgatc gttgattttt tttgtttcgg attaactgtt actaccatca tcgtatcaga
     4921 acgtccttgt tccgttcttc ctaatccacc ggtatctaaa cctaatagca aaaccgaaaa
     4981 aggttctttg tcatcaatgc ttactctctc agaacgtttg gaggatattc gattagagtt
     5041 tttagaaatt ttgtttactg tttggctcgc ttctcccata aatctgattc cgtatgctga
     5101 taatccggca atcagcaaaa caaatacccc taacaaggtt aatagaactt tttgccatcg
     5161 cttcataata cacctctgt
//
LOCUS       DACEXQ010000181         5166 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.181, whole genome shotgun sequence.
ACCESSION   DACEXQ010000181 DACEXQ010000000
VERSION     DACEXQ010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5166
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.181"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            154..2448
                     /locus_tag="IXH04_002242"
     CDS             154..2448
                     /locus_tag="IXH04_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ1852814.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            2441..3106
                     /gene="pgmB"
                     /locus_tag="IXH04_002243"
     CDS             2441..3106
                     /gene="pgmB"
                     /locus_tag="IXH04_002243"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ1852815.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            3099..4106
                     /locus_tag="IXH04_002244"
     CDS             3099..4106
                     /locus_tag="IXH04_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ1852816.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            4128..5147
                     /locus_tag="IXH04_002245"
     CDS             4128..5147
                     /locus_tag="IXH04_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1852817.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
BASE COUNT         1674 a         1061 c         1034 g         1397 t
ORIGIN      
        1 aaacctataa tacgtgtgtt cccttctata attttctcct aaaaatttat ttttttaagc
       61 ttttttctat gaaatatttt cttttgtcta ttgacttgtg caatcggttg cgtttattat
      121 gaacgtataa aacagctcat taggaggaga aaaatgaaac agatcaaacg actatttgct
      181 attgatccat ggaaaatcag cacgacacaa ttagataaaa aaaatcttcg actgcaagaa
      241 tctttaacaa gtattggaaa cggttatatg gggatgcgcg ggaactttga agaaacttac
      301 tcaggggacc accatcaagg tacttatttg gcgggtgtct ggtatcccga taagacacgt
      361 gtcggctggt ggaaaaatgg ttatccagaa tactttggaa aagtgatcaa cgccttgaac
      421 tttatcgcga tagatatttc gatcaacgat cagctgatcg acttggcttc tttggaacca
      481 gaagactttt actgggaatt agatatgaaa aatggtgtat tatctcgtac attcacaatc
      541 actacttcta ctaataaagt acgcatttcc ttcgaacgtt ttctgagcat tgtcaaaaaa
      601 gaatcagctt atatctattt aactatagaa atgcttgaag gtagcggaac agtcaaactc
      661 gtatcaaaat tggatggaaa tgttcaaaac gaagacagca actatgatga acatttttgg
      721 aatgagattg atcgtggaac tatagaagaa atcggctatt tgacgactaa aactattcca
      781 aacaactttg ggattgaaca atttactgtt actgcagcaa tgcgccattt cgttaatgga
      841 aaatcagtca tccctgacta tcacgaagat actttagcat tacatggttg cttgtctgca
      901 gaattatctc aaggtgagac atggtcagtg gaaaaagaaa ttatcgttct tactagtaga
      961 gatattccag aagcgcagca agcagaaaaa gctgtctcag aattaacgaa ccagacaaac
     1021 aattatatcc aagcaaaaga agagcaaaca gcggcttggg cagaacgatg gaagcttgcc
     1081 gatgtggtca tcgaaggaga cgaagaagca caacaaggta ttcgattcaa tttgttccaa
     1141 ctattctcta cttactacgg cgaagatgaa cgattgaata tcggccccaa aggattcaca
     1201 ggagaaaaat acggcggagc cacttactgg gatacagaag cgtatgctgt ccctctttat
     1261 cttgctttag ccaaacctga agtaactaag aatctgctga aatatcggca taatcaactg
     1321 ccgcaagcaa tccacaacgc tcagcaacaa ggattaaaag gcgcgcttta tcctatggtc
     1381 accttcactg gtgtcgaatg ccataacgag tgggaaatca cattcgagga gattcaccga
     1441 aatggcgcga ttgcctatgc aatctacaat tatacgaatt acaccggcga tacggaatat
     1501 ttgaaaaatg aaggactgga agttttagca gaaatcgcac gcttctgggc agaccgtgtc
     1561 catttttcaa aacgagcaaa taaatacatg atccacggtg tgaccggccc aaacgaatat
     1621 gagaacaata tcaataacaa ttggtataca aatacgatcg cagcttgggt cttgcgttac
     1681 acgcgagaaa attatttaac ttatcaaaaa gaagctgcta tcactatcag cgaagaagaa
     1741 ctagccaaat ggaaagatat cgcagagaac atgtacttcc cagttgacga agaattagga
     1801 atctttgtcc agcatgacac attcttagat aaagatctca tgcctgtatc cgatattccc
     1861 acaagcgatc ttcctctgaa tcagcattgg tcttgggaca aaatccttag atcttgcttc
     1921 atcaagcagg cagatgtact acaggggatt tatttcttca atgatacttt tacaaaagaa
     1981 gaaaagcaaa aaaactttga tttctatgaa ccaatgactg tccatgagtc ttccctttcg
     2041 ccaagtatcc attcgatttt agcagcagaa ttaggaatgg aaagcaaagc tgtagaaatg
     2101 taccagcgaa cagctcgctt ggatttggat aattacaata acgacacaga agacggcttg
     2161 cacatcactt ccatgactgg tagctggttg tcgattgtcc aaggatttgc tcaaatgaaa
     2221 actgcccatg atcagttgag attcgcacca tttctgccaa aaacttggaa agcttattct
     2281 ttccatatca attatcgcgg acgtttattg ttcatctcag tcacagatga acaagttact
     2341 ttcgagttat tagaaggttc tcctctagaa atttacgtct atgaacagtc ttattcttta
     2401 gcggatcgtc tagaaataac gctaagaaag gaggaagcaa atgtttaaag gggtattgtt
     2461 tgatttagac ggtgtcatta ccgatactgc tgaattccat tatcgtgctt ggcgaaagct
     2521 tggtcaggaa atcggcatta cgatcgacca cacattcaac gaacagctaa aaggcgtcag
     2581 ccgagaagac tcactctctt tgcttttagc ttatggcgga aaagaacact ctttttcaaa
     2641 ggaagaattt gcagaattag cgaaaagaaa aaacgattat tatttagaga tgatccagac
     2701 gatcgaacca aaagatgtct tccccggggt cgtgccttta ctggattctt taaaagaaga
     2761 aaaaatcaag attgctcttg cttctgcaag taaaaacggg ccattcctac ttgaaaaaat
     2821 gggtcttaca ccttactttg atgctattgc cgacccggca gaagcaacaa acggaaaacc
     2881 tgcgccagat atcttcctcc ttgcagcaaa agctgtcgga ctaaaagcag aagaatgtat
     2941 cgggatcgaa gacgcgcaag ccggtatcca agccatcctc tcaagtggcg ctcaaccaat
     3001 tggcgtggga cgtgctgaag accttggaga ggagatcccg cttgtccctg atacgacttt
     3061 tcttacgatc gattacttga aagaaaagtg gcatgaccat ggataatcca gagatctccg
     3121 tcgtaccagt tccagagact gaaataaaga aaatcacatt gaagaacgac tacttagaag
     3181 tcgttcttct caattacggt gcccgcctgc accaaatctt cgcaccagac aaagaaggaa
     3241 aatcagaaaa tgttttattg tcttacgatc gtttcgaaga tgttctaaca gacaaaagct
     3301 tttttggagc aactgtcgga cctgtagccg gaaggatccg ggatgcttct tggggtgagc
     3361 atagattgga gaaaaactgc ggatcacacc atatccacgg tggcacgaaa ggctggtctt
     3421 tccaattttg ggaagtagaa gttttcaaaa ctccatcatc aataggtgtg gtcttttact
     3481 taaaagatga attttctacc tatccaggac caatcactgc tacgatcact taccgtttaa
     3541 caaaaaacga attagagatg acaacctctg ctagtagcct tgttgaaacc atctgcaatc
     3601 cgaccaacca tgcctacttc aacctttcag gaaatggaac aagagacatc tatgaacatc
     3661 agttaacggt ctttttagat ggtgtgcttg aattagatca agaaaaactg cctactggaa
     3721 attggacaaa aaaagaaaaa ctgccaatcg actttagaaa atccccaaca ctgcaagaga
     3781 tccttgcatg ttatccagat ggactagatg atgtatttct tttgcatcat cctaggcttt
     3841 ccaaaacttc cttacaatta ttcgagaagc attctggtcg ccaaatgact atagcaactt
     3901 ctaataaaag tatggtatta ttttctacga ctggattcga agctgatttc tctgtcaatg
     3961 gaaaacagat gcattcaaac tacggattag caatcgaacc gcaagaaatc cctgatatcg
     4021 ttcatttccc caaatttgga tcgatcaacc tccatcctgg tcaagaacgg atcagtcaga
     4081 cgatttatcg gttctctgca caataacaag agaaaggtgt ttttatcatg gctattaccg
     4141 taaaagatgt ggcgaaaaaa gccggtgttg ccacctcgac cgtctctcgt gtcatcaacg
     4201 atcatcctag tatttccgag acaacaaaga agaaagtccg aaaaattatg gatgaactag
     4261 gttatgttcc taacatgact gcccgcaatc taggaaaacg catttccagt gccatcggcg
     4321 taatcttacc ccctcttgat tcaaaagagc ggatcggtaa cccattttat cttgagatca
     4381 tggaagcaat caacgaagaa gcgcggaatt atgatatgac tacagcaatc gctactgcta
     4441 aaaattttga attgctgtta gagaacgtcc agcgtatgca tttacaaaaa caagtagatg
     4501 gttttattct agtgtattca gatagtcaag atactgtcat tgattattta taccaaaacc
     4561 acatcccttt cactttgatc ggacagcctt atcatcatga gaacgaaatc gtttatgtag
     4621 ataatgataa ccagcttcta ggtaaacaag caacagaatt tttgatccag aatggacata
     4681 agaatatcct gtttgtgaca aacacgactc atgaaagttt gtatttcgaa cgatatttcg
     4741 gttatcaaaa agcaatgatg atggctggtc ttcctgcgtt atccagtgta gtactagagc
     4801 atccggagga ttatctgtcc tttgagaaaa cgttgaaaga aaacgaagcc acagcattgg
     4861 tggtcattga cgatatcttc gcccttcgca cgatgcagtt ggcgcaaatg tatggctatg
     4921 tcattccaga gacactctcc gtcattagct tcaacaactc gatcttctca acattaacac
     4981 acccttacct tacaagtatc gatatcgata tctccgaatt aggacgtgtc gctatgcaaa
     5041 agctgcgcga attgatccat gaagaattga gcagtggcgt acgtttggtc atccctcatc
     5101 gcttgatcaa aagagagaca gtcgttcctt tagaaaaccc atactaaata gctgactcac
     5161 taaata
//
LOCUS       DACEXQ010000182         5155 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.182, whole genome shotgun sequence.
ACCESSION   DACEXQ010000182 DACEXQ010000000
VERSION     DACEXQ010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5155
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.182"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..1414
                     /gene="hflB"
                     /locus_tag="IXH04_002246"
     CDS             <1..1414
                     /gene="hflB"
                     /locus_tag="IXH04_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAQ1852818.1"
                     /translation="KTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETAK
                     KNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATNR
                     SDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGFA
                     GADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYHE
                     AGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGGR
                     TAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQT
                     KAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKSL
                     FEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEENK
                     AEHDSDSQQSEDNHSDDQYK"
     gene            1631..2518
                     /gene="hslO"
                     /locus_tag="IXH04_002247"
     CDS             1631..2518
                     /gene="hslO"
                     /locus_tag="IXH04_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAQ1852819.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            2519..3523
                     /gene="dusB"
                     /locus_tag="IXH04_002248"
     CDS             2519..3523
                     /gene="dusB"
                     /locus_tag="IXH04_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAQ1852820.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            3634..5130
                     /gene="lysS"
                     /locus_tag="IXH04_002249"
     CDS             3634..5130
                     /gene="lysS"
                     /locus_tag="IXH04_002249"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAQ1852821.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
BASE COUNT         1784 a          913 c         1190 g         1268 t
ORIGIN      
        1 taaaacctta ttagcaaaag ctgttgccgg agaagctgga gtaccgtttt actcgatctc
       61 gggttctgat ttcgtagaaa tgtttgtcgg tgttggagca agtcgtgttc gtgatttgtt
      121 tgaaacagcg aagaaaaacg caccagctat cattttcatt gatgaaattg atgcggtagg
      181 tcgtcaacgt ggtgccggta tgggcggcgg acatgatgaa cgcgaacaaa cattgaacca
      241 attgcttgta gaaatggatg gcttcgacgg aaatgaagga gtaatcgtca ttgctgcaac
      301 caaccgttct gatgtactag acccagcgtt actacgtcca ggccgttttg accgccagat
      361 tttagttgga cgtccagatg taaaaggtcg tgaagcaatc ttgcgagtcc atgcacggaa
      421 taaaccgatg gcagatgacg ttgacttgaa agttgtggct caacagacac caggttttgc
      481 cggagcagat ttagaaaatg tcttgaatga agcagcgtta gtagctgcta gaagaaataa
      541 aaagaaaatc gatgcttctg atatcgatga agcggaagat cgcgtgattg caggtccagc
      601 taaaaaagat cgcgtaatca gcaaaaaaga acgcgaaatg gttgcttatc acgaagctgg
      661 tcatacgatt gtcggattag tattgagtcg cgctcgggtc gtccacaaag taacgatcat
      721 ccctcgcgga cgcgccggcg gatatatgat cgctttacca aaagaagatc agttcttgat
      781 gacgaaagaa gatatgtttg aacaaatcgt aggcttactt ggtggacgta cagctgaaga
      841 aatcatcttc aatgtccaat caactggtgc ttccaatgac tttgagcaag cgacagctct
      901 agcacgaagc atggtaacag aatatggaat gagtgatcgt ttaggtcctg ttcaatatga
      961 aggaaaccat caagtgtttg ttggccgcga ctatggtcaa acaaaagctt actcggaaca
     1021 agtcgctttc gagattgatc aagaagtacg aaaaatcttg atggaagctc atgataaagc
     1081 tcgagaaatc atcgaagctc atcgtgcaca gcataaactg attgctgaaa aacttttgga
     1141 attcgaaaca ttggatgcca aagcaatcaa gtcattgttt gaacacggtg tgatgcctga
     1201 aggtactgag tcaaatgatt atccaagcga aaaagaagcg aaaacttttg aagaagcaaa
     1261 acgtgctttg gaagaaaaag acgctgaaaa acaagcggaa gaaaaacgtg attttgaaga
     1321 agcaaaacat gaactgaatg aggaaaataa agcagaacat gattcagatt ctcaacaatc
     1381 agaagataat cattcagacg atcaatacaa ataaaaaaat ataggtcaat tgatctgata
     1441 tgactcccga aaaccggaac aaaaaccggt tttcgggaga tttctttatt taagttgtaa
     1501 atagggttat gtcctgtttt ctagatgatt ctgaattatt tttaaaagaa gcattccaaa
     1561 gttgaaaaaa gcagtagttg ctagtatgat tcactatgag tttaaaaacg gaaaaaggag
     1621 tacataacaa atgaaagatt atttggttaa ggcacttgct cacgacggat tcgtccgagc
     1681 atacgccgtt caagcaacaa ataccgtagc ggaggcacaa agacgtcatg atacttggca
     1741 cacctcctca gctgctttgg gacggacatt ggtcggtagt ctgctacttg gtgcgacgct
     1801 caaaggagaa gacaaactaa ctgttcgaat ccaaggcgat ggtccagcaa caggaattgt
     1861 agtagatgca aatggaaagg gagatgtgaa aggttacatc aaaaatccgc atctcagtct
     1921 tcctctaaat agtgaaggta aaatcgatgt gcggggagca gttggaacac aaggaatgtt
     1981 tactgtcaca aaagacttag gattaaaaga acctttttct gggcaaacgc cgattgtttc
     2041 tggagaaatc gctgaagatt ttacttacta tcttgctatc tcagaacaaa tcccttcagc
     2101 tgttggactg agtgtattgg tcaatactga cgacagcatc aagacggcgg gaggattcat
     2161 gatccaaatc atgccaggtg ccgatgaaga aacaatcagc aagatcgaag aacaattaaa
     2221 aacgacacca agaatctcta ccctcttaga tgaaggacag acaccagaag aaatattgaa
     2281 aggattgctt ggtacagaag aattgaacat tttggaaaaa atgcctgttc aattcaaatg
     2341 tgactgttca aaagaaaaat ttgcagaagc aattatcgcg ttaggtgcag aagaaatcca
     2401 agcaatgata gacgaagatc acggtgcaga agcggtttgt tctttctgta ataacaaata
     2461 caagtatagc gaagaagaat tagatggatt gaagaaagaa gccttaggag ggatttaagt
     2521 ggaacacaca tggaaaattg gcaatgtgga aatccctaat cgtgtcgttg ttgcaccgat
     2581 ggctggaata acaaattcag catttcgagt cactgtcaaa gaatttggtg cgggcttagt
     2641 cgtatgtgaa atgatcagtg accaaggtat tcatttcaga aacaaaaaaa ctttagagat
     2701 gctccacatt gaagaaacag aacatccatt aagtttacag atatttggcg gaaataaaga
     2761 tagtctagtt gaagctgctc aatttgtcga agaaaacaca gctgccgata taatagatat
     2821 caatatgggc tgtccagtaa ataaagtaat caaagcagaa gcaggagcaa aatggctttt
     2881 agatcctgag aaagtatacg aaatggtcca agctgtttct tcagcagtaa atatcccagt
     2941 aacaatcaag atgcgtattg gttgggatga agagcacgtc tttgctgtag aaaatgcatt
     3001 agcagcacaa tcagctggag caagtgccat cgctatgcat ggacgtacac gggttcagat
     3061 gtatgaagga aaagcggact gggaagtatt gaaagaagtg aaaaaacatt tgacgatccc
     3121 atttatgggg aacggggatg tcaaaacgcc agaagatgca aaatggatgc ttgactatgt
     3181 cggtgcagac ggtgtgatga ttggtcgtgc agcgctgggc aatccatgga tgattcaccg
     3241 cacacagcat tatctcgaaa caggagaact gattcctgaa ccaacaccag ccgaaaaaat
     3301 tgctacagct aaacttcatt tacaacgctt agcagatctc aaaggagaaa aagtagcttg
     3361 tagagaattc agaacacatg cggcttatta tttaaaaggg attcctcgag cagcgaaagt
     3421 gaaagtagca atcaatcaga cagaaaaaca gcaagaaatc aatgacctgc tggatcaatt
     3481 tgtagaaaaa gcggaaaaag tactattgaa acaaacgttg tgatttctaa gtttttcgtg
     3541 aattttcaag ccgaaattcc aagttcggct tgcttttttt gtgtttcatt ggcattatag
     3601 gaaagagatt aacatgagaa tggaggaact tttgtgacac aggaacaaca acatcaagta
     3661 gaagatttaa atgatcaaat gcttgtacgt cgtcaaaaaa tggaacaatt gcgtgaagat
     3721 ggaatcgatc catttggaaa acgatttgaa cgcacgcaca actcagaaga attgcatgaa
     3781 ctatttgatc caagaacaaa agaagaacta gcagaaatgg gattgaccgc tagtgtagct
     3841 ggtcgtatga tgactcgccg aggaaaaggg aaaactggat ttgcgcatct acaagatcgt
     3901 gaaggccaaa ttcaaattta tgtaagaaaa gaccaagtag gtgatgaggc ctatgaagta
     3961 ttcaaacatg cagaccttgg agatttcttt ggtgtaactg gacaaatcat gaaaacagac
     4021 acaggcgaag tttctatcaa agcttcagaa atcacgattt tgtcaaaagc acttcgtcca
     4081 ctgccagata aataccatgg cttaacgaat attgaacaac gctacagaca acgctacttg
     4141 gatttaatca gtaatcgcga aagctttgac cgatttatga aacgaagcca aattatcagc
     4201 gaaatccgtc gctatttaga tggcaatggc tatattgaag tagaaacacc agtattgcac
     4261 aatgaggcag gtggagctgc cgcacgccca tttatcacgc atcataatgc actggacatt
     4321 gatttatatt tgcgtattgc gctagaactt catttaaaac gactaattgt tggcggaatg
     4381 gaaaaagttt atgaaatcgg ccgcgttttc cgtaatgaag gaattgatac tacacataat
     4441 ccagaattta cgatgttaga agcatacaca gcatatactg atttcaatga tgtaatgaat
     4501 ctgacagaag gtattatccg taacgcagct gaaaaagtac taggaacagc aaaaatcaca
     4561 tatgatggac aagctgtcga cttagaaagt gactttaaac gcatccatat ggtagatgca
     4621 atcaaagaac aaactggtgt agacttctgg caagaaatga cattggaaga agcattagct
     4681 ttagccgaaa aacacaatgt agaagtaacc gaagctatgg gtgtcggaca tgttatcaat
     4741 gaattctttg aaacatttgt tgaagaaaca ttgacacaac cgacgtttgt atacggacat
     4801 ccagtcgaag tttcgccttt agcgaagaaa aatccagaag atcctcgatt cacagatcga
     4861 ttcgaactat tcatagtggg gcgcgaattt gctaatgcct ttacagaact gaacgatcca
     4921 attgatcaaa gagaaagatt cgaagcgcaa gaaaaagaac gtgaactagg aaatgacgaa
     4981 gctcacggtg tagacgaaga cttcttggaa gccttagaat acggtatgcc gccaactggt
     5041 ggtctaggca tcgggatcga cagattagta atgttgctaa ctgatgcaca atcaattcgg
     5101 gatgttttgc tattcccaac aatgagataa tcgaataaaa gctgtctaag gaatt
//
LOCUS       DACEXQ010000183         5153 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.183, whole genome shotgun sequence.
ACCESSION   DACEXQ010000183 DACEXQ010000000
VERSION     DACEXQ010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.183"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..182)
                     /locus_tag="IXH04_002250"
     CDS             complement(<1..182)
                     /locus_tag="IXH04_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1852822.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="IXH04_002251"
     CDS             517..951
                     /locus_tag="IXH04_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ1852823.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="IXH04_002252"
     CDS             974..1294
                     /locus_tag="IXH04_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852824.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="IXH04_002253"
     CDS             1435..1626
                     /locus_tag="IXH04_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852825.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="IXH04_002254"
     CDS             complement(1697..1954)
                     /locus_tag="IXH04_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAQ1852826.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="IXH04_002255"
     CDS             2112..2375
                     /locus_tag="IXH04_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852827.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="IXH04_002256"
     CDS             2399..2587
                     /locus_tag="IXH04_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852828.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="IXH04_002257"
     CDS             complement(2670..2855)
                     /locus_tag="IXH04_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852829.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="IXH04_002258"
     CDS             3070..3441
                     /locus_tag="IXH04_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ1852830.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="IXH04_002259"
     tRNA            complement(3591..3663)
                     /locus_tag="IXH04_002259"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="IXH04_002260"
     CDS             complement(3709..4137)
                     /locus_tag="IXH04_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852831.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="IXH04_002261"
     CDS             complement(4197..4955)
                     /locus_tag="IXH04_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAQ1852832.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT         1800 a          795 c         1002 g         1556 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt tcagctgttt
     4981 atctttccta ttataaatgt atttttattg gaaatggaag tattatattt taatatgtag
     5041 gaaataatta gtttttaagg aatatgtttt aattttttga aaaaaattct tctaaataca
     5101 acagctgttt taattgtgaa aacgaaaaca gtacgctata atgcctgtaa ggt
//
LOCUS       DACEXQ010000184         5081 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.184, whole genome shotgun sequence.
ACCESSION   DACEXQ010000184 DACEXQ010000000
VERSION     DACEXQ010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5081
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.184"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..1649
                     /locus_tag="IXH04_002262"
     CDS             <1..1649
                     /locus_tag="IXH04_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HAQ1852833.1"
                     /translation="KLAGISVPKGTKMLIAELEGVGPDYPLSREKLSPVLAMIKANNT
                     DHAFDLCEGMLNLGGLGHTAVIHSENEELHVKFGLRMKACRILVNTPSAEGGIGDIYN
                     EMIPSLTLGCGSYGKNSVSRNVSAVNLINVKTVAKRRNNMQWFKLPPKIFFEKNSLLY
                     LEKMENVERVMIVCDPGMVQFGYCDTVREVLARRKNDVKIEVFSDVEPNPSTNTVYAG
                     TKLMADFKPDTVIALGGGSAMDAAKGMWMFYEHPDTSFFGAKQKFLDIRKRTYKIDKP
                     EKTQFVCIPTTSGTGSEVTPFAVITDSETHVKYPLADYALTPDVAIVDPQFVMSVPAS
                     VTADTGMDVLTHAIESYVSVMASDYTRGLSLQAIKLVFDYLEKSVKTPDMESREKMHN
                     ASTMAGMAFANAFLGICHSVAHKIGGEYGIPHGRTNAILLPHIIRYNAKDPQKHAMFP
                     KYDYFRADTDYADIAKFLGLKGETTEELVEALATAVYELGKSVGINMSLKAQGVTQET
                     LDTTVDRMAELAYEDQCTTANPKEPLISELKQIIIDAYNG"
     gene            1878..2984
                     /locus_tag="IXH04_002263"
     CDS             1878..2984
                     /locus_tag="IXH04_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAQ1852834.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            3001..4025
                     /locus_tag="IXH04_002264"
                     /pseudo
     CDS             3001..4025
                     /locus_tag="IXH04_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            4031..4510
                     /locus_tag="IXH04_002265"
     CDS             4031..4510
                     /locus_tag="IXH04_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAQ1852835.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKARLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            4526..>5081
                     /locus_tag="IXH04_002266"
     CDS             4526..>5081
                     /locus_tag="IXH04_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAQ1852836.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGA"
BASE COUNT         1597 a          901 c         1148 g         1435 t
ORIGIN      
        1 caaaattggc aggaatcagt gtaccaaaag gaacgaaaat gctgattgct gaattggaag
       61 gcgtaggccc agattatccg ctatcaagag aaaaactttc tccagttctt gccatgatca
      121 aagctaacaa cacggaccat gcgtttgatt tgtgtgaagg tatgttgaat cttggaggat
      181 taggacatac agcagttatc cattcagaaa acgaagagtt gcatgtcaaa ttcggtcttc
      241 gaatgaaagc ttgtcgtatt ttagtgaata caccatctgc agaaggcggc atcggtgata
      301 tctataacga aatgattccg tcattgactt tgggatgcgg ttcttatggt aagaactctg
      361 tttctcgtaa cgtctctgct gtaaacttaa tcaatgtcaa aactgtagcg aaacggagaa
      421 ataacatgca atggttcaaa ttaccgccaa aaatcttctt tgaaaagaac tctctattgt
      481 acttagagaa aatggaaaac gttgaacgtg tcatgatcgt ttgtgaccct ggtatggtcc
      541 aattcggtta ctgcgataca gttcgtgaag tattggcacg acgtaaaaat gatgtgaaga
      601 tcgaagtctt ctcagatgtt gaaccgaacc catcgacaaa taccgtatac gcaggaacga
      661 aactgatggc agacttcaag ccagatacag taatcgctct tggaggcggt tctgcaatgg
      721 acgcagctaa gggaatgtgg atgttctatg aacacccaga cacatctttc tttggtgcaa
      781 aacaaaaatt cttggatatc cgtaaacgga catataagat cgacaaacca gaaaaaacac
      841 aattcgtatg tatcccgaca acatcaggga ctggttctga agtaacgcca tttgcggtca
      901 tcactgacag tgaaacacat gtgaaatatc cgttagcaga ttatgcattg acgccagatg
      961 tcgcaatcgt cgatcctcaa ttcgtcatgt cagttccagc atctgtcaca gcagataccg
     1021 ggatggatgt gttaacacat gcaatcgaat cctatgtttc agtcatggct tcagattata
     1081 cacgtggatt gagcctacaa gcaatcaagt tagtctttga ctatttagaa aaatcagtaa
     1141 aaacacctga tatggaatca cgtgaaaaaa tgcacaacgc ttctacaatg gctggtatgg
     1201 cgtttgctaa cgcattccta ggtatttgtc actcagtcgc acataaaatc ggcggggaat
     1261 acgggatccc tcatggacgg acaaatgcga ttcttttacc gcatattatc cgctataatg
     1321 caaaagatcc acaaaaacat gcgatgttcc ctaaatatga ctatttccgt gcagatacag
     1381 attacgcaga tatcgcaaaa ttcttaggtc tgaaaggcga aacaacagag gaattagtcg
     1441 aagctttagc aacggctgtt tatgaattag gaaaatctgt cggcatcaac atgagtctaa
     1501 aagctcaagg agtcacacaa gaaacactag atacaaccgt agaccgtatg gctgaattag
     1561 cttacgaaga ccaatgtacg acagctaatc cgaaagaacc attgatctct gaattgaaac
     1621 agatcatcat tgatgcttac aacggataag caaaagaaag cgtagaaaaa agataagaaa
     1681 atagatgaaa aagaacttgg aaaacgttgt ttctccaagt tcttttttta atttccaagt
     1741 aactgaaagt tacttttgtt atttaacatt taggtcaatt ttagacagtt tttgttcaaa
     1801 aagcgatatt tatagtggac ggtattactg ctatattaag gatataaaat gaaagcgttt
     1861 aaaaaggtgg cgtaaagatg attattaaag aagagatttt tgtacctaaa gaattagtga
     1921 aagaaaaaat tgacctgctc gttgaaaacc tgacaacgat caaagacgat aatggtgaat
     1981 ttttgttgga ctttgatgga ctgaaagtag atgacaaaag ctggacagtg tggaattggc
     2041 cacaaggtgt cggtctatat ggtatttata aaaattacca gatgacaaaa aatcctcgcg
     2101 cttatcaagt ggtcaatgaa tggtttgagg atcgtatgga agaaggcgct ccgcccaaaa
     2161 acgtcaatac aatggcaccg ctattgacta tggcttactt gtatgaagac acgaaagatt
     2221 cacgctattt gccttattta gaacaatggg cagaatgggt catgaatgac atgcctcgga
     2281 caaatgaaga cgggttacag catgccacat atggaccgga aaataagaat cagttgtggg
     2341 acgatacatt gatgatgaca gtgcttcctt tggcaaaaat cgggaaactg ttgaatcgcc
     2401 cagaatattt agaagaagct cgttaccagt ttatgatcca tatcaaatac ttgatggata
     2461 aacgaacagg attgtggtat cacggctgga cgtttgaagg aaatcataac tatgccgaag
     2521 cattttgggc tcgtggaaac tgctggttga cgattgctat tccagagatc attgaaatct
     2581 tagaattatc gaaagaagat gcattgcgca aattattgat cgaaacactt gaagcacaag
     2641 tgcgtgcatt aaaaacttat cagtctgaaa gcggtttatg gcatacgttg ttggatgatc
     2701 cttcttctta cgtagaatct tctgcaactg caggatttgc ttacggtatt ttgaaagcgg
     2761 tccataaacg ttatttacca caagaatata aagaagtcgc ttatcgtgct atccaaggat
     2821 tgcttgaaca aatcgatgaa aaaggagaag ttcaaaacgt ttcaatcggg acaggaatgg
     2881 gggacagttt agatttttat cggaatatcg gaatcacagc aatgccttat ggacagtcgt
     2941 taacggtgct ttgtttgaca gaattgctcg tctcttattg ttaaaagaaa ggaaacccat
     3001 atgaaaaaaa catcactgct gtttgcagca agttcgctta gcctaggtct gctaggtcta
     3061 agcggctgcg gaaataattc tagtgtggca actgtcgcag atgcagacgt aaatgagata
     3121 gttaccttgc gctttgctta tgcaagcaac agccagtaat tgatgcaatg aatgaatttg
     3181 ggcgtttagt aaaagaaaaa acagatggtt ccgtacagat ccagtatttc ccagatggac
     3241 aactgggtgg agaacgtgaa ctgatcgaat tgacacagtc aggagcagta gatttcacaa
     3301 aagtaagtgc ctcagcgttg gaaagtttct ctaaagatta ttcgattttt tccgttcctt
     3361 atctattcaa tgatgaagaa catttcttta aggtgatgga taatgaagaa ataatggagc
     3421 caatctacca atcgaccaaa aatttaggat ttaccggatt gacctattat gattctggtc
     3481 agcgcagctt ttatatggta gacggtccga tccatacgcc ggaagatttg aagggaaaga
     3541 agatccgtgt gatgcaaagc gaaaccgcta tcaaaatggt agagctttta ggcggttcgc
     3601 ccgtacctat gggaagtgat gaagtgtaca cgtctcttca atccaatttg atcaacggat
     3661 cagaaaacaa tgaatttgtc ttgtatacag ctggacatgg cggggtagct aaatactatt
     3721 cctatgatga gcatacacgt gtgccagata tcatcatcat gaatgatgcc atcaaagaac
     3781 agctaactga tgaacaggaa aaagccattg aagaagcagc gaaagaatca acggtttttg
     3841 aaattgaagc ttttgcaaaa gcaattgaag aagaaaaagc tttagcacaa gaagaacatg
     3901 gagtccaatt caatgatgta gataatacac cgtttcgaga ggcagttgcc ccacttcatg
     3961 atatattcaa aaacgatcct acctacaaag acctttatca aaagatccaa gagcaaggag
     4021 cgtagctgta atgaaaatga ttttaaacaa ggtattagag atcctgggag ctgttttgct
     4081 tgttgggatg gtcgctgttg ttttatggca agtattttcc agaacggttt taggaaatcc
     4141 aaatacggtc acggaagagc ttgttcgatt cggtcttgtc tggtttgcca tgttgtcttc
     4201 cgcgtatgtt gtgggacaaa aagcacgttt agccgtaacg gttttaagtg aaaaattaac
     4261 tggcaaaaaa agtaaaattt tagaagttgt tgttcaaagt ttatttctgg tatttgctgc
     4321 gatgatcatg gtgtatggcg gatggaatgc tgtcacattg acaatggggc agatctcgcc
     4381 ttctttaagt attcctatgg gatatgttta tctgtcagta cctgtatcgg gggtactcat
     4441 cattatctat agcctgatca atctaatcga tacattaagc aacaagccag caattcaaga
     4501 aaaaatataa aaatggagga ataaaatggc aatagaagcc ggattagtgt tattttttgt
     4561 atttgttgtg ttgcttgtat tagggatgcc gattgctata agtattgcat tctcatcgat
     4621 gatgacttta ttgttagtta taccgtttga tgtttctgct tttagttcag ctcaaaaaat
     4681 ggtgggaagt atcaatagtt tttcgctgat cgctattccg tttttcgttc tctcgggcat
     4741 tatcatgaat aatggaggga tagctaagaa actagtggat tttgcgatgt tgttcgtcgg
     4801 gcgcgtgccg ggggctttgg ctcatacgaa tgtgttaggg aatgctttat ttggttccat
     4861 ctctagctcg gccattgccg cttctaccgc gattggtgga gtgttgatcc ctcagcaagt
     4921 ggaagaagga tatgaccgga agtttgcgac agcggtcaat atcgcgtcag ctccgacagg
     4981 tatggtcatt cctccaagta ctgcttttat catgtattca cttgttgctg gtggtgcttc
     5041 gatctctgct ttgtttatgg gaggttatct cgtaggcgct t
//
LOCUS       DACEXQ010000185         5064 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.185, whole genome shotgun sequence.
ACCESSION   DACEXQ010000185 DACEXQ010000000
VERSION     DACEXQ010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5064
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.185"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            301..585
                     /locus_tag="IXH04_002267"
     CDS             301..585
                     /locus_tag="IXH04_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852837.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="IXH04_002268"
     CDS             588..1004
                     /locus_tag="IXH04_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852838.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            1318..1719
                     /gene="tnpA"
                     /locus_tag="IXH04_002269"
     CDS             1318..1719
                     /gene="tnpA"
                     /locus_tag="IXH04_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_045009167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAQ1852839.1"
                     /translation="MSEIHYGRGYVYSIQYHLVWCVKYRHDVLYGQVDIDVKQFLKQI
                     ADDNNIKIIEIESDKDHIHLLIECTPQHYIPSIVKVFKGASARLLFKKHPELKQLLWG
                     GHLWNPSYFVATVSENTEEQIRKYIQNQKKK"
     gene            1731..2876
                     /gene="tnpB"
                     /locus_tag="IXH04_002270"
     CDS             1731..2876
                     /gene="tnpB"
                     /locus_tag="IXH04_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAQ1852840.1"
                     /translation="MLQHRAYKFRIYPNQEQEILMTKTIGCSRFVYNYFLNLWNHEYT
                     TTGKGLSYNSCSAMLPQMKRNAETIWLKEVDSIAIQSSLKNLSDAFSRFFKKQNKRPQ
                     FKSKKHPIQSYTTKNVNNSIEIRNNFVKLPKLGFVKFAKSQEPKGRILNATIRKNSSG
                     KFFVSILCEEEIVEFPKSNSAIGIDLGITDFAILSDGQKIDNNKFTSKMAKKLKREQR
                     KLSKRALLAKKKGVNLFEAKNYQKQKRKVARLHEKVMNQRNDFLNKLSTEIIKNHDII
                     CIEDLNTKGMLRNHKLAKSISDVSWSSFVTKLQYKANWYGRKIIKVDKWFPSSQICSN
                     CGHKDGKKSLEIREWTCPVCHAHHDRDINASINILTEGLRLHVMGLA"
     gene            complement(3006..3725)
                     /locus_tag="IXH04_002271"
     CDS             complement(3006..3725)
                     /locus_tag="IXH04_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ1852841.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLED"
     gene            4378..4473
                     /locus_tag="IXH04_002272"
     CDS             4378..4473
                     /locus_tag="IXH04_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAQ1852842.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(4775..5020)
                     /locus_tag="IXH04_002273"
     CDS             complement(4775..5020)
                     /locus_tag="IXH04_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852843.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1764 a          883 c          824 g         1593 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttatggacta gccgagaaca cccgtgattt taatcatggg atgaatcgga ttcagttgtg
     1201 acctagcttt agctaggtta aacaactgac aaatataaac taaataatag tgaaatgtct
     1261 tgcaataatt gattttgtag aaatacatat attaaaataa ttgcgaggtg tttttgtatg
     1321 tcagaaattc attatggacg gggttatgtc tattctattc aatatcatct tgtttggtgt
     1381 gtaaaatatc gacatgatgt tttgtatggt caggtagata ttgatgtcaa acagtttttg
     1441 aaacaaattg ctgatgataa taatataaaa attatagaaa tagaatcaga taaagaccat
     1501 attcatttac tcattgaatg caccccacaa cactatattc ctagtattgt taaagtattt
     1561 aaaggcgcat ccgctagact gctgtttaaa aaacatcctg aacttaaaca actcctttgg
     1621 ggtggacatc tttggaatcc aagctacttt gtagctactg ttagcgaaaa tacagaagaa
     1681 caaattagaa aatatataca aaatcagaaa aagaaatgag gtgaatccgt atgctacaac
     1741 atagggctta taaatttaga atctatccta atcaagaaca agaaatatta atgactaaaa
     1801 ctattggctg ttcaagattt gtctataatt attttttgaa tttgtggaat catgaatata
     1861 cgactactgg aaaaggatta tcttataatt cttgttctgc catgcttcca caaatgaaaa
     1921 gaaatgcaga gacaatttgg ttaaaagaag ttgacagtat tgcgattcaa tcctcattga
     1981 aaaatctttc tgatgctttt tctcgcttct ttaaaaagca aaataaacga ccacaattta
     2041 aaagtaaaaa acatcctata caaagttata caactaaaaa tgtgaataat agtatcgaaa
     2101 ttagaaacaa tttcgtaaaa ctacctaagt taggatttgt taaatttgcg aaaagtcaag
     2161 agcccaaagg gcgaatctta aatgctacga ttcgaaaaaa ttcgagtggt aaattttttg
     2221 tttccatcct gtgtgaagaa gaaatagttg agtttcctaa atctaattcc gctattggaa
     2281 tcgaccttgg tataactgat tttgcaatcc tttctgatgg tcaaaagatt gataacaata
     2341 aattcacgtc aaaaatggca aaaaaactca aacgtgaaca acgtaagttg tcaaaacgtg
     2401 cattactagc taaaaagaaa ggcgtcaatc tctttgaagc taaaaattat caaaaacaaa
     2461 aacgaaaagt agcacgacta catgaaaaag taatgaatca acgcaatgac tttttaaaca
     2521 agttgagcac agagattatc aaaaaccacg atattatctg tatagaagac ttaaatacaa
     2581 aaggtatgtt gcgtaatcat aaactagcaa aaagcatttc tgatgtgtct tggtctagtt
     2641 ttgtgactaa gttacaatac aaagctaact ggtacggtcg gaaaatcatc aaagtagata
     2701 aatggtttcc atctagtcaa atctgttcaa attgtggaca caaagacggc aagaaatctc
     2761 tagaaattcg agaatggact tgtcctgttt gtcatgctca tcatgaccga gatatcaatg
     2821 ctagtatcaa tattttgacc gaaggtctaa gattacatgt catgggatta gcttagatcc
     2881 aatcatcaaa aaccgtagga actacgggga tagcttggta aataagagaa accgctgtga
     2941 gtaaagaaat acgcttgcaa gtatgctcta ttcccaagaa tctcgtgact ttagtcatga
     3001 gaggttcaat cttctaacca gttacgcaga gaaatctttg gcaaatctga cgttaggctt
     3061 gaaaaagtaa attctaattt ggcgatttta ttgccctttt ttgcttttat ttttgtgact
     3121 ttaagatcat tgaagtattt ttttagttct cgtaatgcag gatttaatac tttttgatca
     3181 atgttaccca tttgatagta ttctgggata tccattaagc tcctaaactc ttcaatacga
     3241 actacataat aaccagtaga acggtactgc ataagcagtc ggaatagagt ttttgcatac
     3301 gtagaacgca acgacgtgaa agcagataat tcaaatctac taaattcagc tgttaaacca
     3361 ttaataatat attctaggtc cggattaaca ctgacttcaa caaacttttt tgttttattg
     3421 atttcaaaac cagtaaacaa aacaaagtag cgaatagaat cctcatcttc agtacggtaa
     3481 gtaagattaa gcatatcttt atagagcttt tctaaatcac cgacaaaggc ttttgtagca
     3541 gtcatcttat aatcacttaa ctcttttaat tcctcgaaag aaaatctaac ttttttcaaa
     3601 ccacgatctt tcatacgagc gcataatgca aaaaataaat ccatctgctt agcattgaaa
     3661 tttttcagcg gtactaaatt caattcattt tgatatttga ctgttaattc cgttttttca
     3721 tccattcctt tcttcccccc ttacaaataa ataataacct ttaacaacca actcgtaaat
     3781 aggttggttg ttattataca aaaggatatt agtactcaga taaaaagttg ttagtactca
     3841 gataaaaagt tgttagtact cagataaaaa gttgttagta ctcagataaa aagttgttag
     3901 tacccttctg tatccgttgg gagagtaagg ctcaactgcc ccctaaaaag ggttctaaaa
     3961 aatctttaaa aaataaataa aataaacgcg cgcgcgcgag caaaatttcc cgccaaaaaa
     4021 tcacccactt tttaaaataa acaggttcgc gcatgatatt aaactgccaa tctagctccc
     4081 tatggtcgct gtaagaagga gtgatcgcta cgctctcaca ttttacttgc agcagtcgcc
     4141 tttaggctcc ttcgccaaaa ataattcatt agggactcct ttgtggcgtc ctgagagctc
     4201 gtctaagccg ttctaagaca agattcgata aaaactatgt ccttcgttct aaagcgcctt
     4261 acaggcggtc agggcaagcc ctaacaaccc caaggcaata aaaaacttag gcttattttt
     4321 gcttttttat tgtataattg attgtcgcct atccgacagt cttgcgactg gaaggaggtg
     4381 tgtttcgtgt tcactaactt tatttgctcc cttcttgttg ggcttttaag cgctttgttc
     4441 agttactggt tgaataaccg aaacaagaag taaaacttaa ttaaggcgac tcagcccaca
     4501 aaaaaagccc cagagaatcc gacctctctg gggctttcgt gtgtttcgtg ttcacttatt
     4561 tgcaatcaaa ttataacaga aataaatatt tatttcaaaa atatcctaca aaagtctaaa
     4621 atccgtaatt atttcttata tgcaacattt acaaataaag tcgctctgag agctcgtatt
     4681 tttcgattta agacgtttta tttagttttt ggtgtatttc attggtccaa gctaaaaaac
     4741 tcttagaagc gattttagag ccatttaaac ccacttaatt atattctcca tgaaattcgc
     4801 tccatgcgtc aaaagctgcg tctgaaattc cgccgtcttc gttcactagt tgcattaatt
     4861 tttccaacga tcgttttctt tcgggtgtca tatttttata ttccaacaat ttttcgtctg
     4921 ttaaatcgaa cttgattgct tgaattgctt gctctaattt ttctaagttt tgaaaatatt
     4981 cgtaaggaaa tttttccatt ttttctttct ccagtctcac aatttttcta gaaataaaat
     5041 cacttctttg ccgtctatac tctc
//
LOCUS       DACEXQ010000186         5058 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.186, whole genome shotgun sequence.
ACCESSION   DACEXQ010000186 DACEXQ010000000
VERSION     DACEXQ010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.186"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            211..1500
                     /locus_tag="IXH04_002274"
                     /pseudo
     CDS             211..1500
                     /locus_tag="IXH04_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(1567..2994)
                     /locus_tag="IXH04_002275"
     CDS             complement(1567..2994)
                     /locus_tag="IXH04_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAQ1852844.1"
                     /translation="MKKTVKDIFYNAIYQVFLIVLPLLTIPILSRRIGSTGLGIYGYV
                     FSISQFLMTVIAVGMNPFRIRNIAKSRKDKKALSLQFWNIYFIQFLIGLSVSFLYIAY
                     IYFFDVKYKNLYLIQLIFIVGLTLDISWFFQGIEEFSKVVIRNTVIKLSSVLLIIIFV
                     STKDDLWIYVFITSITNFLGSLVFWLSIHGKVGKPIFNSNVFKQLWKPAFIILTPQLF
                     MQVYTTLDKTIVGYFVDPTELSYYDQSQKIARIILAVLTSITIVMLPKISKAQAEGKS
                     ENILVYTKKSFDYTIIIALILFSVVFINTKEFVPWFFGENFKPMTINMLISCLIIVVS
                     PIGGVFANQFAIAIEKDKEYAYPMIIGAIVSIIGNVAFVPTYGAVAATIVLVVVELLV
                     FFFRVFFVRKEIKISYFFGKNVILFFIVNLTLTTWAYYFLPQLFSSSFLDMAIKSIMI
                     FIIYLPFVYFLFPDIRNEIRKITRK"
     gene            complement(2991..4133)
                     /locus_tag="IXH04_002276"
     CDS             complement(2991..4133)
                     /locus_tag="IXH04_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid biosynthesis protein F"
                     /protein_id="HAQ1852845.1"
                     /translation="MKNIKKFFSRNIFPVFIKILPIQKNLIVFSSFGGRNYSGNPRVI
                     SEKIHEKKLNYRIIWLIKKEAIITDLPDYIKRIDSDSFLAAFYLYTAKYWIDDSRKTI
                     KYAKRKKQLYFQTWHGTPLKKIEFDAKDKLPKRYLSYAKKDSESITYLLSGNRYSSKK
                     YVSAFNIVPSKILEVGTPRNDELASSKEQVFDLKKKQINVLFAPTFRNNIEDNGITQL
                     EWLGVDNLREFFKKQQQELNFMTKFHPNVHSKLATDSKSIEYMKKHQITLINDGIPLE
                     MLFEEIDVLITDYSSIFFDFALTKKPIILFNYDEKEYSLERGFYIQLSELPIISVQDS
                     YGLVDVFKNQQEKLLKSSQELLDYIGNYETGASTEKVLELIERDIK"
     gene            complement(4137..>5058)
                     /locus_tag="IXH04_002277"
     CDS             complement(4137..>5058)
                     /locus_tag="IXH04_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1852846.1"
                     /translation="NQTHSNIEIIVVNDGSPDRSGEIAEEYKKKDMRLKVIHQVNGGL
                     SAARNSGLDSATGDFVCFIDSDDWIEPDYLELLYFGTQTYGADISVLKIKKISSLEQL
                     KEHTIAERKWEIFDREQAMKNLFINNKIGYSANNKLFNRNLFDNIRFPLGKLMEDKAT
                     TYLLIDHSEKVAVNSSAKYHYYQSPKSILRGSFNPRIFDSFEVHEDILIFIDKFYPEL
                     SINVRTRYVYEAIRMMMTMIKNNYTNKEDYLRSINILQRFEKEVKIDPDFSPKLKKLV
                     KFLLINKNLPYYMSSSKIASKFLIKIESVR"
BASE COUNT         1772 a          853 c          686 g         1747 t
ORIGIN      
        1 cgaacttaat tctttaaata gtgataaata atatttttta aacaatgaaa gaacaatata
       61 tctggataag acgtaaaaaa atggctctat aatttctagg actgatagaa ttttctgtcg
      121 atcctagatt ttcttgtaaa aaaaacagac atacaaaaat ttcgataaaa tagagtcgtc
      181 taaaacatca atctgaaagg aagattttgt atatccattt ctcgttctat caaagagcac
      241 ttcactatta aagataaaaa tattgaaatt aattcttttc aaaaaacttt ttataaggac
      301 aagaaagcca tcgtaatcga aggtacatta gttcgcactt ttagacattg tctattttgt
      361 aaagaaactg cgaagcagct cgtgaaaaac ggcaagaaga tttcaatgat tatcttaaat
      421 cgcagtggga ataaataaac ctatttgcgt cttagaaaac aacattacca ctaccaatca
      481 tgcggaaaat attttactgc tcatacgtat cttgtcgttc cttcatgttt tatttcgaaa
      541 caagttcatt acacgatatt agaagagttg accgagcgtc aagcgttaaa gacacttacg
      601 aaacgttgtg atctttcagt aacgacattt caacggacat tatctgctct atcggcaatg
      661 ctgcgacaga tgctagacta tttacctcat tgtttactat tcgacgagtt tcgctctctt
      721 tcaatttccc atggaaagtt ctccttttct tgtatgaata gagagacagg gaagctcttt
      781 gacattttgt ccagccggag aaaaaaagat ttgattgctt atttcatgcg ttttacaaga
      841 aaagctcata tgggggttcg atacattgtc acaaatatga atgcccctta tttttcatta
      901 gtcaaagagt cctttccaaa cgcccaaatc atcattgatt gctttcacat tggacaacac
      961 ttgaacaagt ttttcgatcg tgttcgaaaa cgtgtgatga agcagttgaa tcaaaaagac
     1021 acttcacaag ctaagtacta tcgacaattg aaacctttgt ataaattact tctaaaatca
     1081 gatgacgagt tggattacac aacattcaag aaatggcaga attttcaatg gcgctattta
     1141 acagaaagtg aagtagttga tcgtctaccc tcaatctctt atgaacttaa aattgctaag
     1201 cagtttatca aaagctgctt agcaattatc acaagaaaca gctagctact tcgagaaacg
     1261 accaaagaag ttccctcgaa acttcactat ctcaaaaagc ctttttcata tctgaaaaag
     1321 gcttttaact agcattttcg aaaaagtatt ctaataacaa aattagagaa tcttcgcaca
     1381 catatcaaaa cattgaaacg atttgcttat gggtttcgca gttttaccaa tatgcgcttg
     1441 tggatttttc taatcaacag gttaatcagc tatgcattaa aagctagtaa ctcttactaa
     1501 ctttgttgtt cataaatgac cttatcagcc cgatttgaca aagaactact tttttacaac
     1561 attattttat tttcgagtaa tctttcttat ttcatttcgt atatctggaa aaagaaaata
     1621 aacaaacgga agataaataa tgaaaatcat tattgattta attgccatat ctaaaaatga
     1681 agaagagaat aattgtggaa gaaagtaata ggcccaagta gtcaaagtaa gattgactat
     1741 aaaaaataag ataacgttct tcccaaagaa ataggatatt tttatttctt ttcgtacaaa
     1801 aaatactcta aagaaaaaga ctaataattc tactaccacg agtacgatcg tagccgctac
     1861 agcgccataa gtaggcacaa aagctacatt tccaataata cttacaatcg caccaatgat
     1921 cattggataa gcatactcct tatctttttc tatagcaatt gcaaattggt tagcaaaaac
     1981 tccaccaatt ggactaacaa ctataattag acaagaaata agcatgttta ttgtcatcgg
     2041 tttgaaattc tccccaaaaa accatggaac aaattctttt gtattgataa acaccacact
     2101 aaaaagaatt aatgcaatga taatcgtgta atcaaaagat ttttttgtgt aaactaaaat
     2161 attttctgat ttcccctctg cctgtgcttt agatatttta ggcaacataa caatagtaat
     2221 tgatgttaag actgccaata taatacgagc aattttttga gactgatcat aataagataa
     2281 ttccgttgga tccacaaaat acccaactat cgttttgtct aaagttgtgt acacttgcat
     2341 aaacaactga ggagtcaaaa taataaaagc tggtttccac aactgtttaa aaacattaga
     2401 gttaaagatt ggtttaccta cttttccgtg gatacttaac cagaaaacta aacttcctaa
     2461 aaagttagta attgaagtaa tgaacacgta aatccacaaa tcatctttag ttgaaacaaa
     2521 aatgattatt aacaataccg atgataattt gataactgta tttctaataa ccactttcga
     2581 aaattcttca attccttgaa aaaaccaaga aatatctaat gttagcccta caataaatat
     2641 caattgaatt aagtataaat ttttatactt tacatcaaaa aaataaatat aggctatata
     2701 gagaaaggaa acactcaagc ctattaaaaa ttgaataaag taaatattcc agaattgtaa
     2761 agatagtgct tttttatctt tccttgattt agcgatattt cgaatacgaa aaggattcat
     2821 tcccacagca atgacagtca ttaaaaactg tgatattgag aaaacatacc cataaatccc
     2881 taaaccagtt gaaccaattc tacgactaag tataggtata gtaagtaagg gtaaaacaat
     2941 caaaaagact tgatatatcg cattataaaa tatatctttt actgtttttt tcactttata
     3001 tccctctcta tcaactcaag aaccttttct gtacttgctc ctgtttcata atttccaata
     3061 taatcaagta actcttgaga acttttcaaa agcttttctt gttggttttt aaaaacatca
     3121 acaaggccat aactatcttg tacgcttata attggtaact cacttaactg tatataaaaa
     3181 cctctttcta agctatattc tttttcatca tagttaaaaa gaataattgg tttttttgtt
     3241 agcgcaaaat cgaaaaaaat agatgaataa tctgtgatta aaacatctat ctcttcaaaa
     3301 agcatttcaa gaggaatccc atcattaatt aaagtaattt gatgtttttt catatattct
     3361 atacttttag aatcagttgc taatttacta tgaacattag gatgaaattt agtcataaaa
     3421 tttaattctt gttgttgctt tttaaaaaat tcacgtaagt tatccacacc taaccattct
     3481 agttgcgtaa ttccgttgtc ctcaatatta tttcgaaaag ttggagcaaa taagacattg
     3541 atttgtttct ttttcaagtc aaacacttgc tctttactag aggctaattc atcgtttcta
     3601 ggagttccta cttctaatat tttagaagga acgatattga atgctgaaac atacttctta
     3661 gaactatagc gattaccaga taataaatat gtaattgact cactatcttt tttcgcatag
     3721 cttaggtaac gtttaggtaa cttatctttt gcatcaaatt caattttttt taaaggcgtt
     3781 ccgtgccatg tttgaaaata aagttgtttt ttcctttttg catacttaat cgttttccga
     3841 ctatcgtcta tccaatactt agctgtatat aaataaaagg cagctaaaaa agaatcggag
     3901 tcaattcttt taatataatc tggtaaatca gttataatcg cttctttttt gattaaccaa
     3961 ataattctgt aattcaattt tttttcatga attttctctg aaataactct tggatttcca
     4021 gaatagttac gcccgccaaa acttgaaaat acaattaaat ttttttgaat aggtaatatt
     4081 ttaataaaaa ccgggaaaat attccttgaa aaaaattttt ttatattctt catctcttac
     4141 ctcacagatt ctatcttaat caagaattta cttgctatct tactacttga catatagtaa
     4201 ggcaagtttt tatttattaa taaaaacttt actaattttt ttaattttgg tgaaaagtcg
     4261 ggatcaattt tcacttcttt ttcaaagcgt tgtaaaatat ttatagaacg taaataatct
     4321 tctttattgg tatagttatt tttaatcatt gtcatcatca tccttattgc ttcatagaca
     4381 tagcgagttc taacattaat tgataattcc ggataaaact tatctatgaa tattagtata
     4441 tcttcatgga cttcaaaaga atcaaaaatt ctaggattga agcttcctct cagtatactt
     4501 ttagggcttt ggtaatagtg atatttagca gatgaattga cagcaacttt ttcggaatga
     4561 tcgatcagca aataagtagt agccttatct tccattaatt tacccagtgg gaatcttata
     4621 ttatcaaata aatttctatt gaataattta ttatttgcag aatagccaat tttattattt
     4681 ataaataagt ttttcattgc ttgttctcta tcaaatattt cccattttct ctcagcaatt
     4741 gtatgttctt ttagttgttc taaacttgaa atctttttta tttttagcac cgagatatct
     4801 gctccataag tttgcgtgcc aaaatatagt aactcaagat agtctggttc tatccaatca
     4861 tcgctgtcaa taaagcacac aaaatctcct gttgcagaat caagaccaga attcctagcc
     4921 gcacttaaac caccattcac ttgatgtatc acttttaatc gcatatcttt ctttttatat
     4981 tcttctgcaa tttccccact tcgatccggg gaaccatcat tgactactat tatttcaata
     5041 tttgaatgtg tctggttg
//
LOCUS       DACEXQ010000187         5038 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.187, whole genome shotgun sequence.
ACCESSION   DACEXQ010000187 DACEXQ010000000
VERSION     DACEXQ010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5038
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.187"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            179..775
                     /locus_tag="IXH04_002278"
     CDS             179..775
                     /locus_tag="IXH04_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ1852847.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            899..2698
                     /locus_tag="IXH04_002279"
     CDS             899..2698
                     /locus_tag="IXH04_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ1852848.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2976..3188)
                     /locus_tag="IXH04_002280"
     CDS             complement(2976..3188)
                     /locus_tag="IXH04_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852849.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(4052..5011)
                     /locus_tag="IXH04_002281"
     CDS             complement(4052..5011)
                     /locus_tag="IXH04_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1852850.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSAASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
BASE COUNT         1487 a          873 c          881 g         1797 t
ORIGIN      
        1 tttaaaaaaa acaagttatt aaataagtct ttagttggat gtttttttga gatcaatctt
       61 ttatacttat gatgaactta caaaattgtt tgttttctgt catatcaaaa aatggcgcag
      121 ttagttgctt ttctagtaaa gtatagttaa caaagaacct ctaaggagtt gatgaataat
      181 gaatattgct gcctctcgaa aactgataaa tttcatatcc attatcggat taggcctatc
      241 aatcgcgctg acgatttatt ttattaattt aggtgttttc aaagatttga atgcgttaag
      301 aggattagta ggtgattcca tcattttagg accgattatt tttgtcttca tccaaatcct
      361 gcaagtagtt attccaatca ttcctggagg gatcagtact gcagctggtg tcctgatatt
      421 tggcccttat gctggattta tctataacta tattggaatc tgcattggtt cgattatcat
      481 ttttctgcta gggcgtcgtt atggaaagcc attcatactt tccatgatta gcgataagac
      541 atataacaaa tatattggct ggctggataa tcaaaaccgt tttgagaagc tgtttgctct
      601 agctattttt cttccagttg ctcctgacga tgcgttatgt ttgatggctg gattgacaaa
      661 catgtcagtg aaacgatata cactaatcat tttgattgct aaaccactgt cgattttctt
      721 atatagtatg gccttgattt atggaggtca atatctaagt ggtcttcttg gataatattt
      781 attttgtaaa attagtcatt gattgacaat gttaaatctg ggaaagggtc tgcaaaacac
      841 actgcaccct ttcctttttc attagatttg cgctacaatg tgaagagcag gtgataggat
      901 gaattatttg aaacgtagct taggtaatgc ttgggacttt ttaaaaggaa cacaagctta
      961 tttccgtgat gtcttgctga tgcatggctt tattttattt atttgtttac ccttattgag
     1021 cagtacgacc cgttttattt tacaccgagg ggcaattgac tatttgtctt atgacaatat
     1081 tccttcaatt ttttctcaac atcctggggt tctactctcg ttaatcgtag ttttgatgac
     1141 gattttactg ttagtttatt tcgaatttac ttttcttttg atgagtgttt tttttattaa
     1201 aaagaaagaa ccaatctctt tgaagcaact cttacacttg acgatacttc agctgaagaa
     1261 agtgagaccc attacttttt tgtttttcct tgcttatttt ttattgattc taccgatcag
     1321 cggattaagt tttaattctg atctactgtc aaaaataaaa atccccgctt tcattatgga
     1381 ttttattttt gctaatcgtt ggatcatcgt cagttccttt ttactcgttt atattttcct
     1441 tggctatatt gggattcgac tgatttttgc tttaccggaa atgattctac gggatcgtcc
     1501 ttttcgagca gccattcggg aaagctggtc attgaccaaa tcgcgtcttt tggccatcac
     1561 gggccaattt attgtgattg gcggtacgat tttactcctt tcttctttag gatatatcgt
     1621 tgttattttg gcacaaagta tggttgaaca atttttccca gactatgcat tgatcagcgc
     1681 tgtgtttgct atgacgcttt tacaagggat tttattattc aatatcgtga tgtctaccgt
     1741 agggattttt tatatcattg ttgatttcat ggatgacgaa ggcttcttgc ctgagatccc
     1801 taagtggttt atcccacaag cccccaactt gcgtttctct gcgctgaaaa acactggttt
     1861 gactcttttt gcagtttttt tcggtattgg cgtctgtctt tacaatatgg attatctgac
     1921 ttcagctgtt cagacaaaac cagtgactgt ctctcacaga ggtgtgagta cccaaaatgg
     1981 ggcgcaaaat actttagaag cacttgaaaa aacaagtaga gactatcatc ctgattatgt
     2041 agagatggat gttcaggaaa caaaagatgg tcatttcgta gtgatgcatg atgcaaatct
     2101 tcgtcactta acaggggtaa atggaacacc acaggatctt acattgaaag aattgacaaa
     2161 tcttacagtt actgaaaacg gagtgcaagc ccctgtttgt tcttttgatt cttatttgga
     2221 aaaagcacat aagctaaacc aaaaattact tattgagatc aagacctcta aaaaagattc
     2281 ggatgacctt gtcgaacgat ttgtccataa ataccgcaaa gttatcttag agaaccatca
     2341 tattttgcaa agtttgacgt accaaaccgt ttcagattta aaagaagcag aaccaaactt
     2401 ttatgtgggc tacatcttac ctttcaatat tgttgggccc cctgtgacgc ctgctgattt
     2461 tctgacaatg gaatatagta cgatcaaccg taacttcatt aattctgccc accaagacgg
     2521 aaaagaagta tatgtatgga caatcaacga ttcagatgct atgagtcgca tgatgtttta
     2581 tggtgtggat ggtatcataa ccgatgagat gggactacta aatgaaagtg tgaaacaaac
     2641 agatgaagaa atcacttatt cagataaact gctaaatttt gttttgggag tcggctaatc
     2701 aaaaatcgtt aatgaaaaaa gtctttatac aacaaaaaac gcataacctg ccaccgcagg
     2761 tatatgcgtt ttttgtttga cgctcataat gtgtgcttaa ctttctcttg attccttcat
     2821 cgcacgaaca atagctaaaa tagcaggaat tgcagagatc actgcaagaa taattcccat
     2881 tttccctgaa ctttttcttt ttggcatatc ttctcgctcc cttctgtctt tatttatatt
     2941 ataagggagt tcttgcgaat tctcaaaaat actgtttaag aaaccaattc agcaggtgcg
     3001 ctaatatcac agaattttac gagcatgcga tacttgcttt cattgataag ttgatagtct
     3061 cgttggtcat aactttcaac atacacgaca gccgtgttga tatatgtacg ttcaatacag
     3121 ccgattagtt catgagatac tccgatcggt ttgcatatat aagatcttcc tacttctgct
     3181 tttaccattt cattccctct tttctttatt tttattttgt tttttcttta ttttataata
     3241 acaaattacc ttgtatttga aaacgttttt atccattttt ttatttttta ttttgagtat
     3301 ctttctgctt tttagataac tattttttaa cacgatctta gacgttgtga caaaaacgtt
     3361 tgtttatatt cctatgttgt tcgtgcgttt ttttctttat attgctaagt tatttataaa
     3421 gaggtgagca tatgatttat attcaagcaa tagtatttgt aaccttgatg attgggctac
     3481 ttatcggatt aaaagaaaat agctaaacca tttaagaaaa aataaataag gaaaatgata
     3541 aatgcttttt aattgcatca aaaaacgtac gatgctaaac ttggtataag ttcgaaagtt
     3601 taaaggaaac tttcaagaac taaattacat tacaaaggag atgaatttta tgacgtttac
     3661 aaaaacaaat atgcaatacc gcgtagcttt tgatacaact tctaaccgtt ttatggcgat
     3721 tgatgcacaa aacgaaaata acgttgcgtt tggtattaca atcgaacaag cagtccgagc
     3781 attaaacgaa tctagataag gagtgaacca ttccaaatgg cgactgatga gatctcggaa
     3841 aatcaataaa agacgtttgt aacggtaatc cgcgacaaac gtctttttgt attcattaca
     3901 tatatttgga aattgtctct ctttatctct agacctaaac aaaaacacaa gttctaatat
     3961 caaccatctc gttttttatg acaattttgt tagctgtttc gatgataata ttccgttatt
     4021 atctgtataa tatttaatat aatcttaatt gtcaaattaa gttagacaga tcatcaatac
     4081 ttacgtaact caaaaatact tccaaaggtg ttcgataatc cagtgatttt ctaggaatat
     4141 tatttcgttt agatgcggca gattgaataa aaaattcatc tactgaattg aaatccatag
     4201 atttcaataa accatctcta cgtaacaatc cgttagaatt ctcgtttagg cctctttgag
     4261 acggcgttcc tggatcagcg aaataaatgg caatatcatt gacattactg atctgtttcc
     4321 aatttgaaaa ttcctttcca caatcaaaag tgatggattt gaataggttt ttcggtacag
     4381 attcaaacca ttgatttaat tttttttcaa tatcaatcgc ttgtctacca cacggtttca
     4441 atgtgatgat aacttttgat aatcgttcaa ctaaggtaat tacagcactt ttatgtttca
     4501 gacctacgat agtgtcacct tcaaggtgac caaactcatt tgagaattgg ctataatcct
     4561 tttcacgttc atgaatagag cggcggaaag tttgttttcc acgtctttct tgatgtccat
     4621 tcggtttacg cttgcctttc atcggtaagt cagaagaatc aaatagcccc tttttgaaca
     4681 tacgataaat ggtacgagca gaacaactaa taggaaacgc tgcacgacca acaatcacat
     4741 cgggtgtcca tccttgaaca acctttcttt gaatatattc tgattgttcc tcgggtaaaa
     4801 caagcggacg tctaccacag tttgatttgt ttttcttata ctgttgataa tattctaagg
     4861 ctgattttcc ttgcttgaag aacagatata ctttatggat cgtttgtctt gaacgattca
     4921 agcaatgggc agttttagca acagattgat tcattttgaa ataagactct attattacaa
     4981 gttcatccgt tgtaagatgg gtataggtca tttgtactca ctccttataa ttctttgg
//
LOCUS       DACEXQ010000188         4987 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.188, whole genome shotgun sequence.
ACCESSION   DACEXQ010000188 DACEXQ010000000
VERSION     DACEXQ010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.188"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..682
                     /locus_tag="IXH04_002282"
     CDS             <1..682
                     /locus_tag="IXH04_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAQ1852851.1"
                     /translation="KDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTII
                     PEAMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQ
                     ALAEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKIS
                     AIETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLK
                     QKLLDLTQ"
     gene            complement(783..2159)
                     /locus_tag="IXH04_002283"
     CDS             complement(783..2159)
                     /locus_tag="IXH04_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1852852.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(2196..3062)
                     /locus_tag="IXH04_002284"
     CDS             complement(2196..3062)
                     /locus_tag="IXH04_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAQ1852853.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(3151..4176)
                     /locus_tag="IXH04_002285"
     CDS             complement(3151..4176)
                     /locus_tag="IXH04_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1852854.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(4194..4868)
                     /locus_tag="IXH04_002286"
     CDS             complement(4194..4868)
                     /locus_tag="IXH04_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1852855.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
BASE COUNT         1599 a         1128 c          846 g         1414 t
ORIGIN      
        1 aaaagacggt ctgctaaaag ataatctgat gttagaacag ctttaccaga aaaacaaaga
       61 ctactttgat gcactgaata tttatatcgc tgctggagaa ttgaaaatgg aagaacttca
      121 aacaacgatc attccagaag cgatgaaaag agcagaagaa actggcgatc agatggatgt
      181 tcagattgcc aatgattaca cgcaattcct agatcgtctg gataaacgta cgcatgatct
      241 tcgcttagct agacagatca cgatccagca agcaccacaa atccgtttga tccaaaacac
      301 aaatcaggcc ttggctgaaa agatccaagc atctatcgct acagccattc cgctgtggaa
      361 aaaccaagtc gtgatcgcat tgaccttgct tcgtcaaaaa gatgcagtaa ctgctcaacg
      421 ccaagtctct gaaacaacaa acgacttatt gaagaaaaat tcagaaatgc tgaaaatctc
      481 agccatcgaa acagcgaaag aaaatgaacg tggaatcgtg gatatcgaga cccttcaaac
      541 cactcaaaat gatttgatcg aaacaatcca agagacattg cgtatccaaa aagaaggaaa
      601 agaaaaacgt cgtcatgcag aagtagagct tggacacatg gaagaagatc tgaaacaaaa
      661 actgttggat ttaacccaat agatcacctt tcgcttgtta ctaaaacaaa cgcccaacaa
      721 cagtttatta caactgttga tgggcgtttt tcttatcttt cttctaactt ctccattgga
      781 gtctatttct cattcatctt gctgtgtcca tagccatagc caaaataaat gatcagtcca
      841 ataaccatta cgataccgaa cacgatccat gtcacagttt gcagacgaag catcaataca
      901 atacaagtca agatggagac gattggcaat agcggaacga atggcacttt gaattctcca
      961 gcttttggtt tgccaaacat ttttctcaac ttcagtaagc ctagactaac aagcatcaaa
     1021 taggcgagcg ccatgatatt cgttagttcc gctaatacat tcaatgggaa aaatccggca
     1081 aagaatacag ctgccacacc agcggcataa gtcgcttttt tcggtgtacg atgcttttga
     1141 tcgatttctg tcataaactt aggaagtaag ccatccttgc tgacagcaaa cagtaaacga
     1201 gcaagtgaat acatcatcga gatcgttacc gtcagtagcg ttaaaattgc ccctacagcg
     1261 atcaccgcgc ccactgttcc atgtcctaca tacctcattg caaaagccac tgggtcattg
     1321 actcctagtt cagtatacgg aacgattcct gtcaagatca gcgtcacgat gatgtaaagg
     1381 acagtagcaa tcagtatcga accaatgatt cctcttggca catctttttg cgggtttttg
     1441 acttcctcgg ctgccattgc aacggcatca aatcccaaga aagcaaaaaa cacaagagct
     1501 gcaccatcta gcacaccgga aaaaccaaaa ggcatgaatg gctgccagtt atcaggttta
     1561 acgaaaaata ttcctacaat gataaacaac gcgatgatac caaatttgac atataccatg
     1621 gcattattca aacgtaatgc tttcttggct tcaagcgaca cgatgtaagt gacgaatatc
     1681 actacgagca ccgcaatcaa gtcaatgtat gtaccatttt ccggattgta cccgccagtt
     1741 aatgcttgtg gaagttcggc ccctaaactt ttcaaaaaac cttgcatata gccagaccat
     1801 ccggaagcaa cagaggaaac cgctagtaaa aattccccga tcaacaacca ccctgtcagc
     1861 caggcagcaa attcaccaaa tacaacatac agataggcat atggcccacc aatcactggc
     1921 actcgcgaag caaattctgc gaaacttagt ccagacaaaa tgacaacgat tgcagctaag
     1981 acaaatgaaa gtgacagtgc tggaccagca ttttgattgg ccgcaacccc tgtcaccaca
     2041 aatattcccg taccgatgat cgcaccaatt ccaagcatga tcaaatcaga cgtttttagt
     2101 tcttttttca tctcactcgt atgattgaga ttgatctctt tcttacgaaa caaatccatt
     2161 gaagttcctc cccattttag gttggaaaac agcgattaaa ctgttttcat ataccctaaa
     2221 aggataccac ctaaaatgac taaaaggcaa ccaacaataa cataaatgag ctcttttttc
     2281 gtttttcttt cgccaaggat gaaaatccca cctaaagtag agataatgat ccccatttgt
     2341 gaaagtgaga agccaacagc taaaccaagt gatttgactg ttagaagcat gcagatgttt
     2401 cctaagcccc ataataatcc acagatcatg tttttccaca caaactgatc gactttaact
     2461 ttacggaaag caaagaagct tgcgcctaag atcattccga tactttgagg taagataatt
     2521 gacatcgcat ccaaattcgc ccagttgacg atgatggtat atacaccata accaatcgta
     2581 gagaagatca atgctctaaa acccttacca aaatcaagca ttttattatc tgtatctatt
     2641 tttccttcgt catcttgtcg ggcagtcaaa tacgcaccgc ttacaagaag agctaaagca
     2701 ataataccgt atacataatc tttcgtttgt gtccattcat ggaagaaaac ggctcccgca
     2761 atcgtattca aaattaattg aaaaccagta gacaatggta gccctacaga aacacccatg
     2821 tacttcattc catggaactg tccattttgt ccaacactcc aagctaagcc ggataaaaat
     2881 ccgatcaaga aaatccaagg ggtgattaca ggactaacaa cgaaaaagac aactaaagaa
     2941 aacagaaatg caccgatcgt cattcctagt gtttgttgat tggcatcgcc accaatttta
     3001 ccacttacta aaccaatact accccatgcc aacataggga ctaatgctat taaaatttcc
     3061 ataaccaatc tccttttatt tcacatgata agaaacttat cagcgaaaac gttaacaaca
     3121 caagcacttt ttttcgtttc taacgatttt ctaacgtttt tcattataaa aatttaagat
     3181 tgggaaaaat aaagtcattg ttgtcccttt tccttcagtt gattcagccg tcaaatggat
     3241 acctaatttt tcggccagat tttttgctaa ataaagtcct aatcctgttg aatgtgtttt
     3301 gctaagtcga ccatttctcc cagtaaatcc cttatcaaag atccgtttca agtcttccgc
     3361 tgggataccg atccctgaat ctttaactga aaggctgact ccttgcgctt ccttttctat
     3421 gctcacaatg atgtttccgt gatccggcgt atatttgatg gcattactaa gcaattggtt
     3481 gaagataaaa gcgatccatt tagcatcggt caaaacagta tgatcctcac caacgatctc
     3541 gtagcgaata tttttttgga taaaataatt tgcctgactg cgtatcacag gctgaatgat
     3601 tccttttagt ccatattctt ggatgaggta gtcccgcgag aaactgtcga gtcttgcata
     3661 atacaaaact tgttctacgt attcatcgat ttttgataat tcattttcta gtaatgtata
     3721 cttttgatcg tcaatatcga actcgatcga acgaactagc aatttagaag cggaaagtgg
     3781 cactttgatt tcatggaccc atgagtcaat ataatctttt tggtcttgtt gattactgat
     3841 tgctgattgc attacttctt ggtgttcacg gatcagctga ttgatatatt cttctaccag
     3901 tttttcttct tctgtcctcc caccactcaa ataattttgc aaaggggaag ttttttgtat
     3961 gtcggctaat tttccccacc atcttctctt gctcaagtaa ccacccagca gataaaaaat
     4021 cagaaagacg ccctcaatca aagctaaata agcaattgtg ctcaaatcaa ctttgatgtt
     4081 tggcgccagc cacatcacaa agatcgtcaa tgcaataaaa aacagccatc ccaagatcaa
     4141 tgtccactga tctttcaaat atttaaaaaa attcatctta accgctcatc ctttcaggga
     4201 acgatatatc cctgtcctac ttttgtttcg atatatcctt ggatacctgc ctgttcgatt
     4261 tttcggcgta accgattgat gttgactgtc aatgtattgt catctacaaa tcgttcgtca
     4321 tcccacagtg ctcgcagcaa tttttcccga cttaagatct ttccattgtg cttcatcaaa
     4381 ataaataata gacgatattc atttttgctc aaatcaatga tctctccgtt gatttccata
     4441 cttccgttgt caacattcaa cgtaatgcca ttgtggctca tcatttcact agataattct
     4501 gtgtagttgt aagaacggcg aagtaatgca ttgatttttg caatcagtac atcgatttgg
     4561 aaatgttttg tcacaaaatc atctgccccc atgttcatag ccatgatcat atccatattc
     4621 gtattcctgc tggagataaa aatgatcggt actttcgata cttcacgtat tttttgacac
     4681 caataatagc catccaaaac gggaagatta atatctagta atacaagctg tggttcttct
     4741 ctttcaaagt ctgagaacac ttgattgaaa tcggtcgttc caaaggtttc aaattgccat
     4801 ttctcaagtt cctccatgat caattgtcga atgatctctt cgtcttcgac taccatgatt
     4861 tttgccataa aaaatccctc catctttctt cttgctacta caatatcaaa gaagcccttg
     4921 tgaaacaagt tggcttacct ggaaaatgtc attttagcaa acgttaaaaa aaactggaaa
     4981 tatgcga
//
LOCUS       DACEXQ010000189         4910 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.189, whole genome shotgun sequence.
ACCESSION   DACEXQ010000189 DACEXQ010000000
VERSION     DACEXQ010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.189"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            253..1689
                     /locus_tag="IXH04_002287"
     CDS             253..1689
                     /locus_tag="IXH04_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ1852856.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVVTTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            1859..3055
                     /locus_tag="IXH04_002288"
     CDS             1859..3055
                     /locus_tag="IXH04_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ1852857.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(3117..3302)
                     /locus_tag="IXH04_002289"
     CDS             complement(3117..3302)
                     /locus_tag="IXH04_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAQ1852858.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            3533..>4910
                     /locus_tag="IXH04_002290"
     CDS             3533..>4910
                     /locus_tag="IXH04_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAQ1852859.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVE"
BASE COUNT         1487 a          863 c         1005 g         1555 t
ORIGIN      
        1 agcatatttt aggtaattaa caagagcaat cagctattcc taacaaaaac tacagttatg
       61 gctatataaa tagctaaaca taagctaaac acgaacgtta agtattattt tcgctttttt
      121 tgttaaaaaa acgttgaaaa gcgaatgaaa cgtctatata atttgaaatt gttcgatgga
      181 tttgccctat tttacttatt ttttattatt atagttttcc atcagaaaaa aattcaggga
      241 gtggaacttt ttatggaaaa gtttttcggt ttaaagaaaa acaatacaaa tgtatcaact
      301 gaaataatgg cgggagtaac gacattcttt gccatgagtt atatcttatt cgtaaatccg
      361 acgattttgt cagcatcagg tatgcccttc caagcagtat ttctagcgac aatcattgca
      421 tcgattattg gtacgctggt aatgggattg ttcgcaaatg taccatatgc tcaggcacct
      481 ggtatgggat tgaacgcatt tttcactttt acggtcgttt ttgggcttgg ttattcttgg
      541 caacaagcct tggcaatggt gtttatttgc ggattaatca atatttttat tactgtcaca
      601 aatattcgaa aaatgattat tcgggcgatt cccgaaagtc ttcaacatgc gattggtggc
      661 gggatcggta ttttcgttgc gtatgtcgga atcaaaaatg caggattctt atctttttca
      721 gcagaccagt cagctatttc gagctctgtc gttgaaggcg gaaaagcgac aaatgttacg
      781 ataaatggtg gaatcgtccc tgcgttagcc aactttgaca acgctcctat cttattggct
      841 gtgattggtt tggtactgac agccatttta gttgtaaaaa atgtccgtgg ggctattctt
      901 atcggtatcg tagtgaccac agtccttggt atttttatgg gtgttgttga tttaagttcc
      961 attgattggc aaacgaattc gttaggtaat tcatttaaag aattaggaac aactttcggt
     1021 gcagcatttg gttctgaagg catgcaatct ttattcagtg attcttccaa gattcctcaa
     1081 gtattgatga cgatcatcgc attcagttta tctgatactt tcgatacgat cggaacattt
     1141 atcggtacag gacgccgcac aggtattttc tcaaaagaag acgagatcgc gttggaagat
     1201 ggtcgtgggt tcaaaacaaa aatggataaa gcactatttg ccgatgcgat cgctacttct
     1261 gtgggggcta tctttggtac atcgaataca acaacttatg tcgaatctgc agctgggatc
     1321 ggggccggtg gacgtactgg tttgacttca gtagtagtgg cagtgctatt cgcattaagt
     1381 agtttattct ctccactgat cgccattgta ccaaatcaag caactgcgcc agcattgatt
     1441 ttagttggag taatgatgat ggcttctttt gcagacatca aatggctaga tatggaagaa
     1501 gctttaccgg ccttctttgc atcgatcttt atgggattgt gctatagcat ttcctatggg
     1561 attgcagcag gatttatttt ctatacaatt gtgaaagtaa tcaaaggaaa atacaatcaa
     1621 gtttctgttg ctttgtggat cgttgatatt ttattcatta tcaacttcat cattctagca
     1681 acaatttaac taaagagaag ggattggaac agtatttgtt ctgatctctt tttgttttac
     1741 ataaaaagaa cgttacatat aaaatgacct ttgcgggatt tttctctttt tactggcgag
     1801 atcgttgctt gtttattcga aaataggtat gataaaagag acgaaagagg gatgatagat
     1861 ggagattaat tggagaaaaa atttagtgat tgcgtggatt ggctgttttt tcactggtgc
     1921 aagtattagt ttagttatgc cattcatacc agtttatgtt gagcaactag gaacaccaaa
     1981 agatcaaatc gaattgtttt cgggactagc aatctctgtg actgcttttg catctgccgt
     2041 ggttgcacca atctggggaa atctagctga tcgaaaaggc cgaaaactca tgatgatccg
     2101 tgcagcagca gggatgacat taacaatggg atctttggct tttgtaccaa acgcgtattg
     2161 gcttttgatc atgcggtttt ggaatgggat tttgtctgga tatattccaa atgccacagc
     2221 gatgattgct tcacaggcac ctagagaaaa gagcggatgg gcgttaggaa cattgtctac
     2281 cggagcgatt gctggaaatc tgatcggacc ttcaatgggc ggtgcactgg ctcagtggtt
     2341 tggtatggaa aatgtattct tgatcacagg ggctttgttg atgatcacaa cggtattgac
     2401 cattttctta gtcaaagaag atttccatcc aatcgagaaa aaagatctga taagcaccaa
     2461 agaaattttt tcaaaaatgg atcatctctc tattttgatc ggtctcttca ttacgacctt
     2521 gattttacag ataggaatca ctacgatcag ccctattttg accttatata ttcgtgaatt
     2581 gagcggaagt acagaaaata tcctttttgt cagcggcttg attgtttcga tagctggtgt
     2641 ttccgctgtc ttttcttcac cgaaactagg aaaactaggg gataagattg ggaatcaaaa
     2701 agttctaatt ggaggactca ttttatcatt ttgctgttat ttacctatgg cattcgtcac
     2761 tacaccgctc caattaggta tacttcgctt tattttaggc ttttcaactg gcgctttgat
     2821 gccttctgtc aatacactga tcagtaagat tacgcctcaa gaaggagtca gccgaatcta
     2881 tagctacaat cagatgttta gcaactttgg acaagtttta ggtcccatgc taggatcgac
     2941 agtcgcgcat gcttataatt acagtgctgt ttttattgtg acaagtttgt ttgttctgtc
     3001 aaatattctt ctttctttat tcaactttcg taaagttctt cgcagcagat tataatggtg
     3061 gtgaggagta gaaaataaaa aagagcttga gaaatctcaa gctcttggct gtttttttac
     3121 gaatgggcag aggtttcttt tcttggcgca actgcaccaa tcaaaaggat aatgacaccc
     3181 gcaatgaatg aaatgattgt tgtcaaagta aaatcatatg ttccgcttgt taatgcagca
     3241 ccaatataac cgactacttg acctagaacg acagcccaga ataatgtaac gatatatcgc
     3301 ataaagaaca cctcttttct atcttaattt taccagattt tctttgaatg taaagaaacg
     3361 agaccaaatc tcaataaaat ttgagttctt gcgaaagaac tcattcctac agattacaag
     3421 caagcaaatg aagtggaata atcaacttca gcgcctgcta tcttaatgaa agtaaaagga
     3481 tcaaaccttt taacgctagt gaattttaac gaaaaaaaag gcggaatgca agatgttttt
     3541 cccacaactt tatacaatta cttcttattc tcttttacaa agtacgatac gtattcgaga
     3601 atatgtacaa gaagcaaaaa aacaaggcta cacttcatta gccataacgg ataaagatgt
     3661 cttatacggt gcacctgaat tctatcgtat ttgtgtagaa gaaggagtac agccaatcat
     3721 tggattacat cttagttata cttatgaaga aaaaaattat ggcttgcttg cgtacgcaaa
     3781 agatgaaata ggttatcagc agctgatgaa actttccagc cgaaaaatga tccaagaaaa
     3841 attggaactt gcagaaatga cagaaaccga ccatctagcg tttgttgttc cggcaaatca
     3901 ttctgtttct gaggctttca ataaagagga taactttttc ggtcattggc agtttttact
     3961 tgaaaatgtt tttactagaa atttttatat cggcttgaca gaaaacacgc ctgatcaatt
     4021 gcctgaatgg tatcagtatg tgataaaaga aaattattcg ctttgtgcat tacatcccat
     4081 cgctagttta caacaagaag aactttttgc acttgaagtg atgaaacata tcaaagaggg
     4141 aagccaaata cctttagaaa atttatctgc atctttacca ctaacagcaa atgtctatct
     4201 tctttcagaa caagaaatga tcaaacgatt cgaaaaagcg ggtctttccc aagctgtaat
     4261 aggcgcccag cgtttagctg aatcgatttc atttgaattc cagttccatc agaaattact
     4321 tccgcattat ccagttccta atggaaaaca agctggagaa tatttaagag agttatgcta
     4381 ttcgcaagtg gaacgccgaa tcactgacta tacaaaggaa taccaagaac gtttggacta
     4441 cgagttatct gtcatccatc aaatgggatt cgatgattat ttcttgatcg tatgggatgt
     4501 aatggctttt gctcatagaa acaaaatcgt taccggtgca ggtcgtggtt ctgctgcggg
     4561 ttcattagta gcctatgtgt tagaaatcac agatgtggat ccattagaat atgatttgct
     4621 gttcgaacgc tttttaaacc ccgaacgctt tacgatgcca gatatcgatt tggatattcc
     4681 tgacaatcga cgtgaagaag tattgaatta tgttcgagaa aagtatggac aatatcatat
     4741 ggcacaaatc gcaacatttg gcacaatggc agcaaaaatg gttcttagag atgcagctag
     4801 agtgtttggt ttatcgcaaa gtgaagcaaa ccgctggtca aaagcaatac ccaatcaatt
     4861 gaaaatcaca ttgacccaag cttataaaac ttcgcatcaa ttagtagaat
//
LOCUS       DACEXQ010000190         4859 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.190, whole genome shotgun sequence.
ACCESSION   DACEXQ010000190 DACEXQ010000000
VERSION     DACEXQ010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.190"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            209..1006
                     /locus_tag="IXH04_002291"
     CDS             209..1006
                     /locus_tag="IXH04_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAQ1852860.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            984..1397
                     /locus_tag="IXH04_002292"
     CDS             984..1397
                     /locus_tag="IXH04_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852861.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            1381..4026
                     /gene="mutS"
                     /locus_tag="IXH04_002293"
     CDS             1381..4026
                     /gene="mutS"
                     /locus_tag="IXH04_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ1852862.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            4044..>4859
                     /gene="mutL"
                     /locus_tag="IXH04_002294"
     CDS             4044..>4859
                     /gene="mutL"
                     /locus_tag="IXH04_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAQ1852863.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGY"
BASE COUNT         1662 a          878 c         1116 g         1203 t
ORIGIN      
        1 aaattacggc ttatttctga agaagtagat gaaacaacgt ttattcggga aatgggactg
       61 tgacaagatg tttgtcatag tcccattttt ttattttatt tttcaaaact aagaaatacc
      121 aggaaagagt tccttttgtt tgaccttttc tttcttattg aaaacaacta taatatagag
      181 tgatgttaaa agagaagagg aatttagtgt gcgggtattg tttattggtg atgtagtagg
      241 ttcaatggga cgagagatga ttactgaata tcttcctaga ttgaagaaga agtaccgtcc
      301 gcaagtaacg attgtaaatg gtgagaatgc cgcttctgga cgtggaatca cagaaaaaat
      361 ctataaaaaa tttttgcaag acggcgtaga tgtcgtgacg atggggaatc atacgtggga
      421 caaccgcgga atctttgagt ttgtaaatga agctaaaaag atggttcgtc cggcaaattt
      481 tccggaaggg acgcctggac aagggatggt ctttgtcaaa gtgaaccaag tcgaactggc
      541 agtgatcaac atgcaggctc gttcttttat ggtggacttg gatgatcctt ttcgtaaaat
      601 gaatgagctg gtagaagaag ccagaaaacg tacgccgatc atttttgtag attttcatgg
      661 agaaacaaca agtgaaaaac aagcaatggg ctggttttta gatgggaaag tttcagcagt
      721 ggtcggtaca catacacacg ttcagacaaa tgatgcgcga attttgccaa gaggaacagc
      781 gtatctgaca gacgttggga tgacaggtcc ctatgatggt attttgggta tgcgcagaga
      841 acctgtaatc gaaaagtttc tgactgcatt accaaaacgt tttgaagttg tggaacaagg
      901 ccgatcgatt ttaagcggct gtatcttgga attggatgat acgacaggtc atgcaaaaga
      961 aatccagctg attcagatca acgatgatcg accgttcatg gagtagaaag agaggcagtt
     1021 ttttgcagca ggagccggaa attcaaaaag aagtaaggaa actgacaaaa cttttaagag
     1081 agaatgaaac gatcattcgc tacaaagaac ttgaagaaaa gatccagcaa aaccagtatt
     1141 tagctgagtt gagagaaaaa atcaaacaag cgcaaaaaga tgctgttcat tttgcccact
     1201 atgacaaacc agcagcagaa aaagaagcaa tcaaacaagc ggatcaattc atgcaagaat
     1261 ttgatcagca tccgcttgtt gtagcttata gaaaacagct gttggaagca gatgatctgc
     1321 ttcatcattt aacaactatg atacaagaag aaatcaatgg acaaatagag gaggaaaaac
     1381 atgcctcaaa aaactaaaaa tactcctatg atggaacaat atctaagcat caaggcccaa
     1441 taccaagatg cttttttgtt ctatcgttta ggagactttt atgaactatt ttacgaagat
     1501 gcgatcaaag cgtcacagct tttggaatta acgttgacga gcagaaatcg aaatgcagaa
     1561 gaacctattc ctatgtgcgg tgtacctcac catgcagcac aaggatatat cgatgccttg
     1621 attgaaaaag gctataaagt agcgatttgc gagcaggtag aagatccaaa gacgactaaa
     1681 gggatggtca agcgcgaagt gatccagcta gtcactcctg gtacagtcat ggacagcaaa
     1741 ggattgtcgg caaaagacaa caatttttta acagctgtag ttagtcaagg aaatgaattt
     1801 ggctttgctt atgccgattt aagtacggga gaattgaaga cggctcggtt gcatgatgaa
     1861 gaagctgttt taaatgaagc ctctgcattg cagacaaaag aactcgtatt aggcagtgag
     1921 atcactgata cccttcgtga acagctaagc gggcgtttag ggctagtctt ttctcaacaa
     1981 aatgagatgg aagaaaacgc ggaatttagc tttttgacaa gcgaactagt cgatccgctg
     2041 gaaaaagaag caaccggcaa actattgact tatttatcgg tgacacaaaa gcggagttta
     2101 gctcatatcc aaaaagcagt agaataccaa ccagatcatt ttttgaaaat ggattattat
     2161 tcaaaattca acctagaatt aagccaatct attcggactg ggaaaaagca cggcactctt
     2221 ctatggttgt tagatgaaac aaaaacagca atgggtggcc gtttactaaa acaatggcta
     2281 gaccgtccgc ttatccaggc aaaacaaatt aaagcaagac aagaaatggt cgcttctttg
     2341 ctagacagct attttgaacg tatcgatttg cagtcagcat taacgaaagt ctatgatctg
     2401 gaacgcctag caggtcgagt agctttcggt aatgtcaatg ggcgagactt gatccagctg
     2461 aaaacttctt tagaacaagt gccaatgatc agagggttga ttgaaggaat cgatcaaggt
     2521 caatggcaaa gtctgctttc agaaatgcag ccgatggacc atctcgtcca attgatcgac
     2581 caagctatcc aagatgatcc gccgttacaa ataacggaag gaaacatcat caaagatgga
     2641 ttcaatgagc agctcgatat gtaccgttct gctatgagaa acggaaagaa atggttggca
     2701 gaacttgaag caaaagagcg tcaagaaaca ggaatcaaga acttaaaaat cggatttaac
     2761 cgcgttttcg gttattatat agaaattaca aaagcaaatc ttggaaatgc agaactggag
     2821 aagtatgaac gaaaacaaac tttagcaaat gcagagcgtt tcatcacacc agaactaaaa
     2881 gaattggaga cacaaatcct cgaagcagaa gaaaaatctg ttgatttaga atatcagttg
     2941 tttttagctg tgagagaaga agtcaaaaag gctatccagc cgctacaagt acttgcaaaa
     3001 gcaatcagtg cagtagatgt gctacaaagt tttgcaacga tcagcgaacg ctatcagtat
     3061 gttcggccag aacttgtcag tgacaagcat cagcttctga tcaaagacgg acgacatcct
     3121 gtagtagaaa aagtattagg acatcaggaa tatatcccta acagcgtaga aatggcagaa
     3181 gacgaaatga tcttgctgat tacaggaccg aatatgtctg gtaaaagtac gtatatgcga
     3241 cagttggcat taactgttct gatggcgcaa atgggctgtt tcgttccggc aaaacaagca
     3301 gttttaccta tttttgatcg gatttttacc cgaattggtg caagtgacga cttaattgct
     3361 ggacaaagta cttttatggt agagatgatg gaagccaatc aagccctgcg ttatgctacg
     3421 ccaaacagtt tgattttgtt tgacgaacta gggcgaggaa cagcaaccta tgacggaatg
     3481 gcgttggccc aagcaatcat cgaatatatc catcgtcatg tacaagcaaa aacactcttt
     3541 tctactcatt atcatgaact gacggtactt gagaaagaac ttccgcagct taaaaatgtc
     3601 catgttggag ccgttgaaaa agacggagaa gttgtcttct tgcataaaat gatggatgga
     3661 cctgcggata aaagctacgg catacatgtg gcaaagatcg caggattgcc ttccaatcta
     3721 ctagaacgag cagcagatat cctgcatacg ctagaaaatg gcgataacgg acaccatact
     3781 gttccagctg aagtagaaga gaaaactaaa acagccaaac aggagcaaat aaaaccacct
     3841 gcagaaaaag taaatcaaga ccatatacaa gaccgtgtaa aagaagaaaa ccaacagctt
     3901 tctttattca gtgaattatc cgaaaatgaa acagaagtca ttgaatcact gaagcaaata
     3961 aatttactag aaatgacgcc aatggatgca ctaaacacat tatatgaatt acaaaaacaa
     4021 ctgtaggaag aaggcggata tcgatggcca aaatccaaga attatctgaa cgtttagcta
     4081 atcagatcgc tgcgggagaa gtagtggagc gaccagcttc cgttgtgaaa gaattagtgg
     4141 aaaatgcaat tgacgccggc agtactcaga ttgatatctt agttgaagaa gcgggactgc
     4201 ggacgatcca agtgattgat aacggagaag gcattttggc agacgacgta gagaatgcct
     4261 tcaagcggca tgcgacaagt aagatccata atcgcgacga tctttttcgc atacgaacgc
     4321 tgggctttcg aggggaagct ttgccaagta ttgcctctgt ttctgaaatg actctggaaa
     4381 cggcgaatca ggaagaaaaa caaggcacct atctgtcgct gaaaggcgga gaagttattg
     4441 agcatcgacc agcaccactg cgtcaaggaa cgaagattac cgtcagcaat ctgtttttca
     4501 atacgccagc ccgcttgaaa tacgtgaaaa cactgcacac agaattagcg aacatcgggg
     4561 acatcgtcaa tcgactggca ttgagtcatc catcgattgc ttttcgttta gtacatgatg
     4621 gaaacaaaat gctggcaact gctggaaatg gcgatctgaa acaaacaatt gcgggaatct
     4681 acggattatc taccgcaaaa aaaatgctga aaatcgaagg aaaagatcta gattttgagt
     4741 tatttggcta tgtttcattg cctgaggtga ctcgtgccag taggaattac ttatcgacga
     4801 tcatcaatgg acgttttatc aagaactttt ccttgaataa agcaatcgtt gcaggttat
//
LOCUS       DACEXQ010000191         4810 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.191, whole genome shotgun sequence.
ACCESSION   DACEXQ010000191 DACEXQ010000000
VERSION     DACEXQ010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.191"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(174..926)
                     /gene="xth"
                     /locus_tag="IXH04_002295"
     CDS             complement(174..926)
                     /gene="xth"
                     /locus_tag="IXH04_002295"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAQ1852864.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            1039..1959
                     /locus_tag="IXH04_002296"
     CDS             1039..1959
                     /locus_tag="IXH04_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1852865.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2032..2967
                     /gene="murB"
                     /locus_tag="IXH04_002297"
     CDS             2032..2967
                     /gene="murB"
                     /locus_tag="IXH04_002297"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAQ1852866.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            3265..4743
                     /locus_tag="IXH04_002298"
     CDS             3265..4743
                     /locus_tag="IXH04_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ1852867.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT         1684 a          770 c          929 g         1427 t
ORIGIN      
        1 tttgtttggc tgttcttatt acttgatgca ggacagcttt ttcacaatct ctcgataaac
       61 agtgttcaaa agccagtttc tcggtattat gccaaattta tgcaaaatgt ggaaagccat
      121 tttcacaaaa tttttcttaa tactcgaaac taaacgactt tttcacaacc tctttatttc
      181 aaatctaatt ctactggaca atgatcactg cccataatgt ccgtatggat ctttgcatca
      241 gctagtcctg gctctaaatc tttgctcgtc aaaaagtaat cgatccgcca accagcattg
      301 tttttccttg aattgaatcg gtagttccac caagagtaga caccttcctg ggttggataa
      361 aaataacgga atgtatcgat aaacccgtta tccaacagtc gagaaagagc cgctctttct
      421 tctggagtga atccagcatt tttttgattc gttttccagt ttttcaaatc aatcttttga
      481 tgagcgacat ttagatcccc acacacgatg actggttttt gtttttttag attttccaaa
      541 taattataaa aggcttcttc ccattctaat cggtaatcta aacgttttaa ttcgctttga
      601 gaatttggcg tgtaacaggt tacaagaaag aaatcggaat attccaaagt aatcagacgg
      661 ccttctgtat catggatatc gatacccatt ccgtaagtgg cattcaatgc tggtttttta
      721 gcaaaaatcg ctgtgccgga atagcctttt cgttccgcgt aattccaata ttgataataa
      781 cctggaagat ctaaatcaat ttgtcctgct tggagtttcg tttcctgcag acagaaaaaa
      841 tcggcgtcta actcatgaaa aacttctaaa aaatttttat ttacgatggc acgaagcccg
      901 tttacgttcc aagagataaa tttcaataaa agcacactcc ttcgaattaa tttccttacc
      961 tttgcgatac tattgtacct cattttagta aaaaatggtg taagatgaaa gtaactaaat
     1021 gatccaggag gaaagtagat gaagattggt gtaatcggtg tagggaatat cgcagaaaaa
     1081 gcatatttac caacttatgc gaaaaaacaa ggaacacttg atttttattt tgcaacaaga
     1141 aataaagaaa caaaagaaag aattcaaaaa gagtacgggt tccaacatct atatgaaacg
     1201 atcgatgagc ttttagaaga aaaaatcgaa gcatgtatga tccacgcagc aacaaaagta
     1261 cattatgaat tagccaaaaa atgtctagaa aatggtatcc atgtttatat tgacaaacca
     1321 ctgagtgtcc atctaaatga agttcgtgaa ttgcaagaat tagctgataa gaaccaaata
     1381 attttgatga tcggtttcaa ccggcgcttt gctcctatgg tagaagaatt gaaacgtatc
     1441 ccagataagc ggttgatcca cctgcaaaaa aatcgaatag cggcaaaaga aacgacagag
     1501 ttcgtccttt atgatttgtt tttacatctt attgatacag ctgtgtattt attagatgaa
     1561 cccattttga gaacaaaata ccagataaga gaaacaaagg aatttttgga atatgccatt
     1621 ttacaattag aaacacaaaa tcaaacggct attttaacga tggatttagc aagtggcgct
     1681 aatatagaaa aatatcaagt aacaagtaaa aatggtactt atgaagtaaa cgatctgacc
     1741 cagcttgtca ttcaagatgc tgatggaaaa aaagtgaaag aatttggcga ctggacaaac
     1801 acgctagtca aacgcgggtt tgagccaatg gtagaggcct tcttttctca gatccgttta
     1861 ggtaaatcgg aaacagaatt gcttaaacaa aaagaagttg taagaagtca tgaattatgt
     1921 gaggaagtgc ttaaagatca ctaccgtcat caactttgaa caagactggc aagattgtat
     1981 taagcgtgat agaataatga ggacgtttga gaaaagaaag gaaaatcctc agtgaataaa
     2041 gaagaaatag ttgaaagctt agccacgatt actatattgt ttgatgaacc attgatgaac
     2101 tatacgttta cgaaaacagg tggaccagct gatgtccttg cattcccgaa aaaacaagaa
     2161 gaagtaaagc aaatcatcga ttattgccga atccatgata taccttggat ggtattagga
     2221 aatgcaagca atctgatcgt tcaagatggc gggattcgcg gtgtagtcat catgctgact
     2281 gaaatgaaac agattcatgt caaaggaacg atggtcattg cggaagcagg tgcctcactg
     2341 attgatacga cttatgcagc tttggcagaa tcattgactg gatttgaatt tgcttgcggc
     2401 atcccgggaa gtgtcggtgg agcggtttat atgaatgcag gtgcgtatgg cggagagatc
     2461 aaagatgttt ttgctgaagt ggatcttttg cttgaagatg gaacattgaa aactttgaca
     2521 aaagacgaaa tggtattttc ttatcgtcac agtaaagtcc aagaattacg tgctatcgta
     2581 ttggaagctc gattcagttt gcaaacaggg gattatgaag caatcaaagc aagaatggat
     2641 gagttgaccg aattgcgtca atcaaaacag ccattagaat acccttcatg cggaagtgtc
     2701 tttaagcgcc cagtaggaca ctatacaggt cagctgatcc aacaagcagg attgcaaggc
     2761 ctgaaatggg gcggtgcaca agtctctgaa aaacatgctg gattcatcgt caatatcgat
     2821 catgcgacag cgacagatta tattgaattg attgcgcaca tccaagaagt aatcaaagaa
     2881 aaatttgatg tgtctttaga aacagaagtg cggatcattg gagaaaaaca agcaatatca
     2941 gaaacaagca gaagtttagc caattaataa gtaatggaac ttaaaataag taaaagagca
     3001 ggacggtggc cgtcctgctc ttttttgtgg ttgtttacgc aaatggcgca aatcttgtac
     3061 attttaacac aataaaacaa aaaatataga atatttcctc ataaattttt ccattttttg
     3121 caatattctc aaaaaaaaga taaaaaatga gaataactga tgaaaaaaca agcataatcc
     3181 atcataaaat cagtataggt gaaaagatga actttcatct gcatatcaag taattggtat
     3241 gaggcatttt ttgaggtgta ggatatgaag ttggattacc ttatagaaga ggaagataga
     3301 gaaaaactgc tgctagctag atatttagaa tttgagaaaa agaattatgt agtgctgcct
     3361 aagttatatg aaacattagg tttgtctaaa tttaaaacaa gaaattatat catagaatta
     3421 aataaagaat ttactgaatt caaaacaaag ccagagatcc aaatttcaga agaagcagag
     3481 atccatgtcg aacgtctgaa tttgacggat atcaaaaatt taagaagaat ctactttcaa
     3541 cgatcaaaaa ttgctcgact gttaacggag atcgtggatt caaaagtaac gatcgaaaaa
     3601 tttgcagaga atcatttttt aagcatatca agagcctatg taaaaagaag agaattagtc
     3661 tcttttcttg aacagcatca aataaagtta aagaaaaaca tattagttgg ggaagaaata
     3721 aacatacgga acgtattatt tggggtatat ttcggggtct ttaatggttt ttctatgcca
     3781 tttagagaaa gcgtcatgaa tgaagcaaag agtatcgtgc aatattttga atatctgttc
     3841 aatttaaaac tgtctgcaac aaaacgaatc aaattcaatt tgctaaccac tattagtttg
     3901 ttgagggcct atgggaatca accaatcgga aaacgtttct tttcagatga atactttaag
     3961 agctcagaag gaatgaagat catcaaggat ttatcacata tttcttttct tcatatccct
     4021 aaagagcaga tcattgatga gatttcttat atcatgttgt ttttgatcac ggaagaagaa
     4081 tttgaattag atggcgcatt tggacttgcg gaagctcatt ttcatgatat ccacatgatt
     4141 agtcaaaaaa tgtcagtgga attgattgat caattaccat tttcgattga aatctctgaa
     4201 gaagaaagac tatatatacg aaaagaactt cgtatgggta tgatgcatgt caatcgaaaa
     4261 aattggatat tcgactttga gatgactagc tttacgtcta aaaagcagct acagtttttc
     4321 ttcgagacgt atccaatatt cagtacagca atccaaaagg tcatacaaga gaataaacgc
     4381 tatttcttgg gaaaagatga acgtttcatg attcgcttat tttatgatta tctcttttta
     4441 ttggtcggta tttgtccggt atcatatttt gaagcaccga ttcatgtctg tattgatttt
     4501 tcgcaaggat ctgcttatac cgattacatt atttctcaag tgaaaggatt taaaaattac
     4561 aatattgtga ttgagagtcg aatcacttct cgtacgaata tttatttatc tgattgttta
     4621 cttgaaaaat ttaataaaga acagataatt tggaaaaatc caccgactcc agatgactgg
     4681 gaatattttg gaaataccat catcagagaa aaaaatagac atttaactga aaaaatcagc
     4741 tagtatttat ggatggcagc tttgtctcac atatttatta gctgatttat gtttttttca
     4801 aagaaaaggt
//
LOCUS       DACEXQ010000192         4798 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.192, whole genome shotgun sequence.
ACCESSION   DACEXQ010000192 DACEXQ010000000
VERSION     DACEXQ010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.192"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(14..484)
                     /locus_tag="IXH04_002299"
     CDS             complement(14..484)
                     /locus_tag="IXH04_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAQ1852868.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(643..1101)
                     /locus_tag="IXH04_002300"
     CDS             complement(643..1101)
                     /locus_tag="IXH04_002300"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAQ1852869.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1272..2416)
                     /locus_tag="IXH04_002301"
                     /pseudo
     CDS             complement(1272..2416)
                     /locus_tag="IXH04_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
     gene            complement(2429..3346)
                     /locus_tag="IXH04_002302"
     CDS             complement(2429..3346)
                     /locus_tag="IXH04_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAQ1852870.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3774..4337
                     /gene="efp"
                     /locus_tag="IXH04_002303"
     CDS             3774..4337
                     /gene="efp"
                     /locus_tag="IXH04_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAQ1852871.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT         1513 a          862 c          888 g         1535 t
ORIGIN      
        1 tatttttatt aagttaaaga acggattcaa ttgcagcggt cattttcaat ggagaagttt
       61 gtggtgcaaa tcgttcgatt actttgccat ttctatcaat caaaaattta gtaaaattcc
      121 attttattgc tttagtgaca gtaccaggag cttcattttt aagataagta aaaagaggat
      181 ctgtattttc tccatttacg tcaatcaatt gatgcatagg gaaggtaacg ccataagtgg
      241 ttttacaagc ttctgctgct tcagtcgctg aatctagctc ttgtttaaat tgattggaag
      301 gaaagcctaa gataaccaga ccttgtgtct gataagcttg gtaaagttct tctaattgtt
      361 taaattgagg tgctagaccg cattttgttg cagtgttaac aatgattatc ggtttgcctg
      421 catatttttt caatgaatag acagtgccgt cttctaaagt agtttcaaaa ttataaatag
      481 acataaagtt ttctcctttc tattttatat aataaaaaga gcgctaaact gaaaactagc
      541 tatttgtttt cccgtttaga actcttttca gacaataaat atttaataaa atacgaagat
      601 aagatagcta tattgcttct catctttacg ttcttttaga aactatgctt ctacttcaga
      661 ccattcatcg cgttttgcta aaaattcacg agccaaagct tgtgcacctt ctagtgtatg
      721 gctggcagcc cagccacatt gtgtttcatt gcttgcaggt acttcagtag cttcaagaac
      781 atctttcatt gttgcttcaa gaacatctaa aatgtcttcg tagctttcat gattcaatac
      841 agtcaagtaa aagcctgttt ggcatcccat tggagaccag tctacgatgt aatctgcgtg
      901 attgcggatt agttcggcag tcaagtgctc aagtgaatgt agacttggca tgtccatgtg
      961 ttctttattt ggttgtttaa agcggacgtc gtatttaatg atgacatcgc catgcgcacc
     1021 cgtttttcga tcagctacac gtacataagg tgctttgact tttgtatggt caagattaaa
     1081 actttcgaca ttcattctca tttgtaagac tcctatctgt ttgttcattc gttttgatgc
     1141 ttttcactaa agaattattt agtgaaattt acaaaaacgt ctttctactt gattctaaca
     1201 cttatcgttt ccaaggtgta gcaatctgaa atatttttta gatttttctg ctaagtcgtt
     1261 tattgggact tctagttctt gattctgtca aatgcctgag ctaaatcgtt tatgagatcc
     1321 tgaccatact caattcctac agaaagacgg atcaagccgt cctttatgcc agcctcttct
     1381 cgtttttctt ttggaataga cgcatgagtc atgacagaag gaatttccac taaactttct
     1441 acgccaccta aactttctgc taaagtaaat agttggagtg attcaacaaa tggaatagca
     1501 gattcttcat tttttaaagt aaaagaaatc attccgctaa agccgctcat ctgctgctta
     1561 gcaactccat ggagttcatg atcgggtaat cctggatagt agaccttttc tactgctgga
     1621 tgagaaaata gaaaatcagc taccacaaaa gcattttttt gatgttcctc catacgaaca
     1681 cttagtgtct tgattccgcg ctgaagtaac cagctatctt ggggaccaag tacggctccg
     1741 atagcatttt ggtaaaatcc gatctgatcg gctagatctt ttggttagaa gtcactaaac
     1801 cagctactac atcgctgtgt ccgcctagat atttggtacc gctatggaca acgatatcag
     1861 aaccgagatc taaaggtctt tgaaaataag gagtagcaaa tgtattgtca gcaataacta
     1921 tcaatccatg ttgatgagcc agagtggcac ttttttctaa atcagtaatc tttagaagcg
     1981 gattgctagg tgtttcaaga taaagtgctt ttgtattggg tttgatggat tgttctattt
     2041 tatcaagatt actcgtatct atgatcgtgt actcgagacc atttttagta agaactttgt
     2101 caaatagacg gaatgttcct ccatagacat catctcctaa taagatatga tcgccagctt
     2161 gaaaaagaga aaaaacggca tgaatcccag aaagaccaga agaaaaagcg aatcctcgaa
     2221 caccgccttc caaatcagca atcagttctt ccaatgcaaa acgagtagga ttaccagatc
     2281 ttgaatattc atattgttta ggctggccga cgccatcttg ccgataagtg gatgtttgat
     2341 aaataggtac actgacagct cctgttgtgg gatcttcact gatgcctccg tggatcaatt
     2401 ttgtttgaat gtgcatgttt ctttcctcct ataagtaaat gttttttgat aaatagcggt
     2461 ctgctgcgtc tggaaaaatc gtaacgacac gatgaccagg tggtaaacga cgaatttctc
     2521 ttaatgctgc tgcaaaagcc gcaccactcg aacttccaac taatagtcct tgttctcgtg
     2581 ctaactgcct agtatagtta aacccttcta catcagagat tgtctcgatc tgattgattg
     2641 aaaggggaga aagaaacggc ggaatgaatt ctacaccgat accctcaatt tcgtgagggg
     2701 ctggatcacc accatttaac acagagccct ctggttctac accgattatt cgaatgtcgg
     2761 gatatttttc tttcaaaaag gtagaagttc cagcaaaggt tccaccactc ccaattcctg
     2821 ctacaaaact atggatattt tctttgattt catgaaagat ttctggccct aacgtatgat
     2881 agtaagcagc aggattatct ttattttcaa attgaagagg cagatagctg tttgatatct
     2941 cctctgcaag ttttttactt ttgctgatgg caccgatgat tccttgctca ctaggagaat
     3001 ggatgatctt tgctcccaat gctttcatca attgttgttt ttctaagcta aacttctctg
     3061 gaacaacaaa aaccgttctt aatttgtatg tgagtgcggc cagagcgatc ccaattcctg
     3121 tatttccggc agttggttcg atgatcgtcg ttttttcatt gatttttcct tgggcaaatc
     3181 cttctttgat caaataactg cccaatctat ctttgatact tcctccagga tttaaatatt
     3241 ctagttttgc ataaatagca gaacctttag gcatagaata gtgctgattg tcaaagcgat
     3301 aaagcggtgt ggaaccaata gcttctgtaa ttgtcgagat aatcatgtgg gcctcctaaa
     3361 aaaagataat aaaaaaagga gctgactatt aaatcagctc cttgaaagac atagagaaaa
     3421 attgagtaga taaaaagata agttacctgg tactcattct atgaagggca attaatagac
     3481 atgacaaata gtatgctgct tttatgcacc atacaacagc aacgattcaa ttgtttttgg
     3541 ttgctgactg tcatgtgtat ccctccaagt aaaattttta ttaacgatag catgaaaaaa
     3601 aataaaagtc aaaggcttga aagaaaaaaa tatttcgata gcatcgttta cccgtgttta
     3661 tttttctgac aaataggcat gagaccttaa aagatagaga atactctagt tttctggccg
     3721 gttgtctgat acaataaagt tgattatgat aaagaaaaga aggagcaaaa ataatgatct
     3781 cagcaagtga tttaagagcc ggtatgactt tcgtacaaga cggcaaactg attaaagttc
     3841 tggatgcaag ccaccataaa cctggtaaag gaaatacagt tatgcgtatg aaactgaaag
     3901 atgtccgttc aggtgcaaca tacgacacaa cgttcagacc agaagaaaaa tttgaaaaag
     3961 cacatatcga cacaaaacct gtacaatatt tgtacacgat ggatgataca gctttcttca
     4021 tggatttgga aacatatgag caatatgaaa ttcctgtaga aacagtagaa gaagaaatga
     4081 aatatatcct agaaaacatg gaagtaaaaa tccaattttt cggtagcgaa gtcatcggcg
     4141 ttcaattacc aacaactgtc gtattacgtg ttgtggaaac acaaccttcc atcaaaggtg
     4201 caacagtcac tggttcaggt aagccagcga caatggaaac tggcttagta gtcaatgttc
     4261 ctgacttcat cgaaacagat gaattgttgg aaatcaatac agcagaaggt tcttatgtaa
     4321 aacgtgcgtc aaaataattc gctaacggat tgataagaat ttgaaaacag agcttgacct
     4381 cttgaaaggc agatcatcgg atctgccttt caagaggttt tttgtggaat cagcaaattg
     4441 aaaaataaaa aagtatttga ttttatttat attttgaaag atgaatggca aaagctagag
     4501 aaagaaaaaa ctgcacttat ttatgaagaa attgaaatga ttcataaaat tgaaaatatc
     4561 cgaactattc cgaactatat tgaagcgaaa atattttttc atcactaaat agtgttgaat
     4621 ttatgttttt atgtataaat agcttctaat agttcgggta ttttgttttt ataaccattt
     4681 ttattcttat ttttaaaaaa atatttcgaa atatactaga caattcagaa aattttgttt
     4741 aatattattc caataaaatt caaatcttta tcaaatgaaa ttccaataaa aacggaaa
//
LOCUS       DACEXQ010000193         4784 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.193, whole genome shotgun sequence.
ACCESSION   DACEXQ010000193 DACEXQ010000000
VERSION     DACEXQ010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.193"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            544..1107
                     /locus_tag="IXH04_002304"
     CDS             544..1107
                     /locus_tag="IXH04_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1852872.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
     gene            complement(1386..1673)
                     /locus_tag="IXH04_002305"
     CDS             complement(1386..1673)
                     /locus_tag="IXH04_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ1852873.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(1663..1992)
                     /locus_tag="IXH04_002306"
     CDS             complement(1663..1992)
                     /locus_tag="IXH04_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007723369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1852874.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            2297..2626
                     /locus_tag="IXH04_002307"
     CDS             2297..2626
                     /locus_tag="IXH04_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1852875.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            complement(2913..3398)
                     /gene="vanZ"
                     /locus_tag="IXH04_002308"
     CDS             complement(2913..3398)
                     /gene="vanZ"
                     /locus_tag="IXH04_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HAQ1852876.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(3551..4462)
                     /locus_tag="IXH04_002309"
     CDS             complement(3551..4462)
                     /locus_tag="IXH04_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HAQ1852877.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT         1564 a          824 c          722 g         1674 t
ORIGIN      
        1 ttaaaacttt tttagcagat tttaaagttt cgtcaaaact aatatattct ggtagctgag
       61 cattcaaata gctttcagac ttcagaagaa aattacgagc atttttatag tccatctcta
      121 ataaagttat tcttttttta tccaatacat tcagctcaat tccttataat attctattcc
      181 ttagtatctc aacattattt actttataaa acgaataaat tttgtagttc taattcaaat
      241 attgcatgtt atatctaata tgggagcatt ttataatttt cattgtgata aactcgttat
      301 taactttagg atacactagt taggattaaa ttaggtatct agaaatttac ttaagatttt
      361 tttgtttatt tcgtatacac actcgtatca aaaacaatcg attatgagac aaaatgaaat
      421 gacctctttt attaaataaa agaggtcata ttttgtatca taacttgttg cattaactat
      481 gtatctataa ctccctttta tgatacaatc aagccagaat taaagtacga aaggaagaaa
      541 agcatgagtg gatacattta tggttatgcc cgagtgagta cacgttcaca agaactaaat
      601 cgtcaattag acttactcaa tgaacaaaat tgtaatgaaa tcctaactga gaaaatgacg
      661 ggaaccaaag cggttcgtcc ccaactaaat cgtttaaaag ataaaattcg tccgggagat
      721 acggtaattg tagaaagttt ttctcgattg ggtcgtagta caaaggattt gattgacctt
      781 gttacctatt ttgaagaaca ccatgttaag ctggtgagct tgaaagaaaa cttcgatacc
      841 agcacgcctc acggacgctt aatgatgact gtcttccaag catttagtca gttcgaacgt
      901 gatttaattg ttgaacgtac caaagaaggt ttgaaaagtg cacgtgcgag aggtcgtaaa
      961 ggtggccgtc cacgagtgaa ccagaaagat gttgatcgag cagtaaaatt gtataaaagt
     1021 caggtttata gcgtaaaaga aattacggaa atgactggta ttagcaaggc aacactctac
     1081 cgctacttaa aagacaatag ggaataatct attttaatct ttaggaatct ataatattta
     1141 tgggagaatt agagtgattt ttttattatt actaaaaaac aatataaaat aacgcatttt
     1201 aagccctctt tgtgcataag gatgaagctt ttaatttgaa agaggcttaa aatgcgtttt
     1261 aattttttct atttttcata aatatatagg ttagtggttt aaaattatag ggtattacaa
     1321 ctctcagctg ttaaaaatta tcttttaaag tgtcgtctat cctctcaagt tgaaacattg
     1381 ggttcttacc taatcttttt taattgttca taaaaatttt catgcgttcc acatagtaag
     1441 ataggtatca ctgcattatc tttaatttca tatgcaacgc gatatgtcgt acctgcatac
     1501 ttgaatccaa tagcccaaat tcctgataaa tcacccgttt tttgttctcc cgaatgagga
     1561 cgaactgcta tctcatcata aattatcgtt aaaaattgtt cttttaatgt tttctctttt
     1621 aattttttta gatactttat tacgtgttta gaaggtagaa tctcataagc caatttctgc
     1681 tgccaactcc tcttttgtca tcggtttaga atttgcaaca gtatcttcag caatagattt
     1741 aaatgcagaa gttaacgcat tttttctatt tctaaattct tttaagatct cttgtttgct
     1801 ataaccttct gaaagaatat cagccaaaat atcttcacta aaatcaaaaa attctttttt
     1861 aggctctaca aactcataaa ggataccatt ctctacaagc ttagcttcta cttcaacacc
     1921 attgggcaca tcgaattctt tagggaccgt taacataata gaatttcctt gttttcttgt
     1981 ttttgtaatc atattatttc ctccattatt ttacatttcg taattactta gtaattacat
     2041 tgtacgctat ttttagtaga aatacaaaat aataaaactc ctttagaaca attttcaaag
     2101 gagttttatt tgattaggaa attatgtaga ataataatat gtttttaact tggaatcaaa
     2161 aacgcacttg caagtaagtt tacttcaata catgaaattg cttataatag tgtattggaa
     2221 ttaaggtaga ttgacaaaag agtgatcaaa taggtatact tcattcaaat aatcgtttga
     2281 atgtgaggtg agatgaatga aaaaagtttg ccaagtgact gtcgtacata aagaaaaagt
     2341 aaatggaata aaaaataaat tggatcaaaa agatttttca aacttacttg tgttagggaa
     2401 atgtttcagc gatttttcta gaattaaaat tttctatgct ttagaaacct ataaggaaat
     2461 gtgcgtctgt gatttagcag aaatacttac tgctagtgtc gctacaacgt cacaccattt
     2521 acgtttttta aaaaaacatg gaatggcaaa atcacgtcaa gatggaaaag tagtttatta
     2581 ctctttagcg aatgaagaca tcctttccgc tattcgtgtt ttttaaagtt atcagaaaat
     2641 ctatctatga aatcttagtt tgggggtacc gtcaggaaaa tgcggattta caacgttaag
     2701 gaaatttatt aaagagacca attcctcagc actaaaattg ataattgggc tttctttgtt
     2761 actatagtgt taggtaagct ttttacttta tcaccggttt tcccctcact tcacacctac
     2821 gggcgagttt ccccacatcc cgttttccat ctaatccaat tcattcaata tctttatgtg
     2881 tatgaaagat cagattgctt gatttagaca tcttacacgt aatttattct taaatgggta
     2941 cggtaaacga gcaataatac gagcaaaagt atacctacaa aaataataac tctgtctaat
     3001 tttttttgat tcatatgctt attgcttaaa ccatataatt tcagtccaag aaagcctcca
     3061 acagtatttg taattacatc tgttatgtct gtcgctccaa tagcgaagat aaattgaatt
     3121 atttcaaaag taagacttaa aaccagtaca aaagcaaact taggtaaaaa tccgatttct
     3181 ttaaaattga cattcaaaag caagccaaat ggaataaaga ttataacatt atctatcatc
     3241 tctctgaaat tcccagtagc agtaaatgga gtcaagttaa gacttctttg atgataatta
     3301 aatactgata aaatattgta ttgtaatttg aataacacta accagattag tgtcactaaa
     3361 tataaagcta gcaatcctct agataatatt tttcccaata taatttcctc taattctttt
     3421 gtttaagttg tatcattagt atagcaatgt cctagttaac gtgggtttta aattaaaaag
     3481 ctaacgttac cccaaaagtg ctagtacaat aatgctgttc attaatttcg ttggtttcat
     3541 tccgccatcc ttacctcctt gaattagtat gtgttgatcc gggaaacact gtcacaatta
     3601 caccgtctat attgtttcct gatatctcat aacgaagatt agtcggcaca tgaatggtgg
     3661 tatttttagt aacaggataa taaaagatct catatttttc cccatttaca ctaacagaaa
     3721 tggttttttc ttcttttagg taatccatat attcctcgag aacgaaattc ttttctttca
     3781 taatcgcact atgtggtaaa ccaacatagc gaatatgcca tggttcatat tgaattcctg
     3841 ttaactctgt tttgtcctct ggataacgta aaatgaaccc gtatttccaa gcattttctt
     3901 ctatccactt tccttcaggg gctcgttcca ttttcgtcaa gcttgatcct acatctagtg
     3961 ataaacctga attatgctca ctataacctg ctggtaaggc atactcagcc cccatttctt
     4021 ggtaaagcac actttgctca tcaaagtctc gatagccact attaataata aaatgactaa
     4081 cgccaccctt tacagcatca ttgaccatct ctgaaaattt ttgtgctatt tcttttgaca
     4141 tataaatatt actatcaagc aacccgtatc catttattaa ttcgtcatgt ttagataaat
     4201 tcacgatatc tgacttcaca ctttcttggc gaacaggata tttactattg attaatagca
     4261 gatttccttg ataaacctgt tcttctgtaa ttgttttctc ttgggtattt tcagaagtcc
     4321 cactattttc taaatgttct ttgggatttt gatcataatt ttgaaattcg actttttcct
     4381 gagaaaacag tgcttcatta acgtagtcat aacctaagta tattaagaat aacaataaca
     4441 ataaaaaaaa caacttcttc attttcagtc tcctccttaa ctatatagtt ggaagtatga
     4501 aatatttcta gaatcttccc cttaaacggc acaatctcgt ttgagattgt gccgttttgc
     4561 cccgtaaatt acagtcaatt atttcgccct actggattta ctattatcac caatgtagat
     4621 gaattttcat aagtgatacc catgctgaat ttaacgaggc tgaactgcct gttgacaatg
     4681 ggcggtgtga aataccaccc ctctatgcga tcagtcttat tatcaggcta tcttgccgca
     4741 gcgcacgcag ataatgaccg caatatcgct ctgccttaca tctt
//
LOCUS       DACEXQ010000194         4679 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.194, whole genome shotgun sequence.
ACCESSION   DACEXQ010000194 DACEXQ010000000
VERSION     DACEXQ010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.194"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..1732)
                     /gene="pepF"
                     /locus_tag="IXH04_002310"
     CDS             complement(<1..1732)
                     /gene="pepF"
                     /locus_tag="IXH04_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAQ1852878.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQ"
     gene            complement(1908..2906)
                     /locus_tag="IXH04_002311"
     CDS             complement(1908..2906)
                     /locus_tag="IXH04_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAQ1852879.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3132..3785)
                     /locus_tag="IXH04_002312"
     CDS             complement(3132..3785)
                     /locus_tag="IXH04_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAQ1852880.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4117..4515)
                     /gene="spxA"
                     /locus_tag="IXH04_002313"
     CDS             complement(4117..4515)
                     /gene="spxA"
                     /locus_tag="IXH04_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAQ1852881.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1372 a          979 c          728 g         1600 t
ORIGIN      
        1 cctgtgtcat atcaactcct gcttttttca ttacttccac aggataatcg ctgttacctg
       61 cttttaggta agccaagtag ttctctagtg cctctggttc ctggtttagt attttcttcg
      121 ccagtgctga agcagcagaa aagccagtcg agtattggaa aacatagtaa ttataataaa
      181 aatgcggtat ccgtgaccat tcgaatttaa tttctgggtc ttcttcgact gctggaccat
      241 aatattttgc attcaattta ccataactat cacttaggta ttcactagtc aatggcacac
      301 ctttttcatc ttccgtatgc atgaaatgct cgaattcagc gaattgtgtc tggcggaaaa
      361 ctgttccttt aaacccatcc aagtaatggt tgagtacata agcccgtaca cgagggtctt
      421 tttctgtttc caataaatac tccgttagga tattttcatt cgttgtcgaa gcaatttctg
      481 ctaaaaagat ggagtagtcg ccgtacacat aaggctggtt cgaacgagtg aaataactat
      541 gaacactatg tcccatttcg tggacaagcg taaatagctg atccagcgta tcatgccaat
      601 tcaataaaat atatggattt gtgtcatagc ttcccgaaga ataagcaccg cttcgtttcc
      661 ctttattttc gacaacatcg atccaacgtt cagagaatgc tttttctacg atggccatgt
      721 attcttcacc cattggtttc agtgcttcta aagctttttc ttttgcttct tcgtacgtaa
      781 aggtaattgg agcttcccct aagaccggtg tataaagatc atacatgtgc agtttttcca
      841 cttctaataa acgtttccgt aattccatgt atcgatgtaa caaaggcaaa tgcttgttta
      901 ccacgtctac caaagtatcg tatacacttt caggaatatg attattgctc aaagacgctt
      961 ctctggcaga gctgtaatta cggactttcg ctttaaaatt atgtcctttt atatgtgtgc
     1021 ctaaagtaga agcaaatgta tttctaaatt gttcgtaaac actgtacaac cccttaaatg
     1081 ctgcttcgcg cacccttcga tccgtgcttt ctagcaactg accatacacg ccatgagata
     1141 attggactat ttcaccattt tctccttcaa tcgttggaaa aactagatct gcattattca
     1201 aaacagcaaa tgtatcactt gaagcatcaa agatttcacc tgctccagca agaagagatt
     1261 cctgctcagc agataagaca tgggctcgat tatctactat ttgctggata taatggcgat
     1321 agacttccaa ttttggttct tctttgaaat attgccaaat ctggtcatct gacaattgca
     1381 atatttctgg ttcaaaccag gaaacggctt cactaacttt cgaaaacaga ctgcttgctc
     1441 ttgcataaag cgcttggtaa tctgtatttc cagtgtcttg atcgttttta aggtgcgcat
     1501 atacataaat gacttcagtt tggcgataaa ccctcaatac gaattcaatc gcatttaaaa
     1561 attgagaagc gccttgatca agtgttcctt tgtgtttttc agattgtttt aactcttctg
     1621 ataattccaa atatttctca tcaaactctt ggtcgcttga aaagatcttg gtcaagtccc
     1681 aagttaaatt ttcaggcaat tcttctcgtt ttggcaactg ctttacttcc atgaaatcca
     1741 ctccttttct tcccttagct ctatctatca aaatagatca tagtctaaca tccacatgtc
     1801 acagcatcta acttttctac tcattcatgt gtctgcttcc atcaatttta gcataactaa
     1861 atcttgtttt acatagaaaa ccgattccta gcctattttt atacttacta ttttttcaaa
     1921 gaagacaagt gtatgcattc gttaaaaaac tgctgataga ccatctcttc atcaatcata
     1981 ggaaaaagcc attccctttt atgttctttt atttggcaaa gccatgagtt gaaatcagga
     2041 actttctggt tttccaaggc ttcttcgtac aacattcgat aaagaaaaac ctgttcttgt
     2101 aaataaaaga aaaaccgact tttttgatag atccacgatg atagatgcaa aagatgttta
     2161 tgacgcaagt agcattgttc ttgtatggat actgtattct tttgcttgtg aataagttgg
     2221 cgtgtcagcc atttcttccc atcttcactc actttttcta tttttttcat ttggttcatt
     2281 ttaatttcgt tactagtgaa tatttcttta aggtccctac tagaaaaagt ccagcagcta
     2341 ctgcaaaagg aaatatgccc tgatacagtc tctgtaatat gatgatacaa ataaagcttt
     2401 tgtttcttga gatcagcttt ccagcaatgc actcctcttt ttctattata gtagcagcaa
     2461 cttttttcta tcaatgagaa ttgcgattgt ccaagaaaag aattaccaag tatccaccat
     2521 acttggtaac tgttattcaa ataattttgg gttctttcaa taaatctctg gtgagacagt
     2581 ctagaacatt ggatttctat tgctagattc gccaacaaaa tatccggcct ttgccgcaac
     2641 ctcggcaaat aggcttccaa aaatactggt tctactgact gtcccagcca atccatgaag
     2701 cattccttta actgtaagtg ctcttttgat tctctttcac taaagaccga acagttatct
     2761 gatttttgat gggcaaaatg agcacatttt ttctttccct ttttcaaaat cactcttttt
     2821 cgacagccac gacaataata tttctttcct ttttctgcat gtttagcaaa acagcactta
     2881 ttatattcat tcattgcaag cagcattttt ctccctctga acttatttac gtctaagaat
     2941 ttatttacac gaaaaaagag ggcgtgaaaa agctattctg catcaagtaa taagaacagc
     3001 taaacaaaaa tagttcccaa tatttttcgt ttggttgggc ttgttaccgc agatgcaagc
     3061 ttttgaacac atttatacga aaaaaagact gcgacacaat ttttgtcaca gtctcttact
     3121 ataatgctga tttatacaaa ataaaatttc gttcgctcga tagcattacg cgccatcaga
     3181 cattgtccat attcattcaa tgtttctggt gtacgatcgc tcttttcagc aaactccaaa
     3241 attcgagcaa tctgattctc tacatccgct tggttgagat tttccatcca gaagtgaacg
     3301 actaggtaat agcgctcttc gaataaatac aaatcagtcc aagcagactc tagttcgaca
     3361 tctgttgcaa gttggatcac ttgttcaaaa tcttcaaaag caaaagttgc ttgtctttcc
     3421 tgttcttcta gggcgcttcc agataattct tcctccggat cgctgccaat aatctgctga
     3481 cgaagaaagt cactcacatc ttctgaatca acagtagaat cttctgattg agaaatttct
     3541 tcatccggaa gatttttact gataaacagt tctaatccgt ctccttttgg caaaacttgg
     3601 aaagtgacag cttcgcttcc tcggaactcc tcttcgatat caacttcttc caaaatacta
     3661 taaaagaaat tttcgatttc tttgtgattg ccaagtaaat ccaaaaatga aacgccccgt
     3721 tcctctaaat cttcatgacc aattagtaca cggatcgtat tttcattaat atgttccatt
     3781 tccattttgc tacacctcac tttgccacaa tgtttaatat agattcattg tagcgaaatc
     3841 agcttaaatg taaagaaaaa aaatctcgct gttaagcgaa tttaataaaa ccagcggatt
     3901 tttcaacctt tcaagctatc ttatttaact ataacatttt tggcagacga atactatata
     3961 gccgactttt cagctaattt tccctctcta tttttcgtgc aatcatcatc ttctactacc
     4021 tttttataca tctaatcaag aatacttttt gtaaaaacaa aaaaacttgg aaaaactatc
     4081 tttgtaatag tcttttccaa gtttcgtatt atccttctat aatccagcca tcaattgtgc
     4141 ttgacgtaat tctaattgtc gtacatcgcg cggtaggaac cgacgaattt catcttcatt
     4201 aaaaccaact tgcagacgtt tatcatcaat catgattgga cggcgaagca atccaggatt
     4261 gttttgcaca agttccaata attcttgtaa cggcagatca tctaaatcca tgttcagttt
     4321 ttggaatact ttggaacgtg tcgagatgat ttcttctgtt ccgtcttctg tcatttgtaa
     4381 aatcgctttt aattcagaac tgttcaatgg ttctgaaaaa atattacgtt caacaaatgg
     4441 aatctgatgt tcttgcaacc acgcacgagc ctttctgcag gaagtacaac ttggggaagt
     4501 atataatgtt aacaacgtgc accactcctt ttcgtgataa gaatcacttt tttgtaacag
     4561 aagatcataa taatctccca tctgtttcta ttatacataa tcaactttta aatagctacc
     4621 tttttttcat aattttttta aaaaaatgaa agttatctaa ggttaatata cagtaaaac
//
LOCUS       DACEXQ010000195         4618 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.195, whole genome shotgun sequence.
ACCESSION   DACEXQ010000195 DACEXQ010000000
VERSION     DACEXQ010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4618
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.195"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            79..999
                     /locus_tag="IXH04_002314"
     CDS             79..999
                     /locus_tag="IXH04_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1852882.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            1040..2497
                     /locus_tag="IXH04_002315"
     CDS             1040..2497
                     /locus_tag="IXH04_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1852883.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            2842..3456
                     /locus_tag="IXH04_002316"
     CDS             2842..3456
                     /locus_tag="IXH04_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ1852884.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            3449..>4618
                     /locus_tag="IXH04_002317"
     CDS             3449..>4618
                     /locus_tag="IXH04_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HAQ1852885.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTL"
BASE COUNT         1559 a          785 c          900 g         1374 t
ORIGIN      
        1 ctttgtcttg ttgatgatca caaatacgat cgcccgacgt tttgatagcg attctgcatt
       61 attctaagga ggaaaaaaat gaaaaaaaag aaagtggaga gagtccaagt ccgctctttc
      121 aataagacgg ctaatttttt ctctaatata atgattgcac tctttgctat ttcctgtatt
      181 ttgccattta tatttgttat cgccatctct tttaccgatg agtccatatt gtcgactcac
      241 gggtacagtt tttggccaga aaagttcagt atatttggtt atacgtactt gttcgaccag
      301 atgcaggata aaattttcca agcgcttttc gtcactgtac ttgttactgt tttaggtaca
      361 ctgatcaata gcacggctac ttctttatat gcttatgcga tttcacggtc aaactttcca
      421 ttccgtcgtt tttttaccgt tttttgtttg attacgatgt tgttctcacc agggatggtg
      481 gccaattatc ttgttatgac aaatcttctt cagttaaaag atacgatttg ggcattgatc
      541 ttgccaatgg ctgtcagtcc cttcaatatt attgtgatgc gcacattctt taaacgttcg
      601 gtctctgatt cgattattga atcggcacgg atcgatggag ctagcgaact gcgaatattt
      661 gttcagatcg ttttgccgtt agctgttcca ggtattgcga caatcagttt atttgccgca
      721 ttgggttact ggaatgactg gttcaatgct ttactataca tccaagatga taagctagtt
      781 ccactacagt atcttttgat gaaaatccaa agtaatattc aatatctgac acaaaattca
      841 ggagcaggaa gccaattgac aggcggatta gcctcagttc caggagaatc tgccagaatg
      901 gcgatcgtag tcatctcgac gttgcctatt gcgatcagct atccattttt ccagaagcat
      961 tttgtcaaag gtctaacaat cggcggagta aaagaataaa gaaacaaaaa tataaatcat
     1021 aaaattagga ggaagaaaaa tgaagttatg gaagaaacta gcctttacag gtgtttcagc
     1081 gttgttgttg ggaacattag cagcatgcgg aggttctggt tctaaagacc aggcagaagc
     1141 tagcaattcg gacactttgc agatgtatca aatcggagat aaaccagata atttcgatca
     1201 attgatggaa gtagcaaaca aacgaatcga aaaagagatc ggtgtaaaag tcaatatcaa
     1261 ttacattggc tggggcgatt acgaaaagaa aatgaacgtc atcatttctt ccggggaaaa
     1321 ttatgatatc gcctttgcga acaactatgt ttccaatgcc caaaaaggag cctttgctga
     1381 tttgactgaa ttagcgccta agtacgctga aaaagcttat aatgatttag atgaagcata
     1441 tattaaagga aatctggtca atggaaaatt atatgctttc ccagtcaatg gaaatgtctt
     1501 tgctcaacaa gtacttactt ttaacaaagc tttactagat aaatacgatc tttcaattga
     1561 tggaatccaa tcttacgctg atgcagaaaa agtcttgaag gaattccatg aaaaagaacc
     1621 aaacacggca gcttttgcca ttggacaagg cttcaaagtc caagggaact ttgattatcc
     1681 tttagggaat acgttaccat ttgctattga tctggatgga gatgattcga aaatcatcaa
     1741 ccaatacgat aatgaaacgt atatcggact gttacgtaca atgcatagct ggtacaaaca
     1801 aggactcatt ccaagtgacg ctgcaacgag caataaggat tatccactag aaggaaatac
     1861 ttggctaatg cgtggagaaa cacaaggacc gtatgattat ggcgatacaa ttttgacaaa
     1921 tgcagcaaaa caagaacttg tctcaaaagc aattactgtt ccactaaaat ctacagctca
     1981 agcacaaatg gcaaattttg tcgtatcaaa tacttcaaaa aataaagaaa aatcagtaga
     2041 acttctaggg ctgcttaaca gtgatccaga attattgaat gggcttgttt gggggatcga
     2101 gggagaagca tgggaaaaag tcgatggttc agatgataaa atcaaattgc tagatggtta
     2161 tcagccaaat acacatatgt ctgcttggaa tacaggaaac aacaagattc tatatacaca
     2221 agagtctatt acggatgaca tgattgccaa acgtgatcaa tcgattgctg atgcagaaac
     2281 ttcaccgatt ttaggattca gcttcaacac tgacagtgtc aaaacagaat taagtaatat
     2341 ttctaatgtc atgaatcaat atttagatgg attgaataca gggactgttg atccagatga
     2401 aacattaccg aaattgaaag acgctttgga caaggctggc tatgataagg tattgaaaga
     2461 aatgcaaaaa caatatgatg agttccgaca agaataataa tgaataaaga caaaggggtt
     2521 gtgacaaaag acgtgtcaca acctcttgtt ccgaataaac ggtgttcaaa agccagtttc
     2581 tcggtattaa gctaaatata tgtaaaaatg tggaaagcca tattcacaaa atttttctta
     2641 ctactcgaaa ctaagcggct atttcacaaa ctcttggtaa acggtgttca atcagctaac
     2701 cctcggtatt cgctcacaaa acgacaaaat atgaggaact atttttccgt ttgtttctct
     2761 aataatcagg tcaaggtgct gattgcccaa cctttttttg tataatgaga aagtataaac
     2821 ttaatgtttg gaggagaaga tatgttaggg aaggttttgg aagagttatt tatccggatc
     2881 tgggtcatta taaaactaac cttgtatttc tggatatata catttgccgg cgggatcatc
     2941 tttggattag gtgcggcatg gaaaacagtc aatgaactat tttatttgta cggattcgag
     3001 tataaagaaa tcacaataaa aagaggctgg aatatatata aaagaaattt tttaagaggc
     3061 aatctgctat ttagtttatt tttatctggt actgctttat tgagttacaa tttatattta
     3121 tctgttcaaa tcaaagggct cattttttta gctattgatt ttattttact ttttgccttg
     3181 ctctgcctat ttgctactta tcaatacagt atgattttag atagtgaata tacgatctct
     3241 gttcctaatc tgctaaagct aagttttatt tcggtttttt ccagcttttc gtcttttcta
     3301 aaaataatta ttggtagcgg tataatttta ggcgtgactt ggcaattcaa aggattgatc
     3361 ctgtttggtg tcattggtct tttgacggtt tggaatggta cgatgacaac acattggcgt
     3421 gaagaattag ataaacagtt ggaaagttat gaataagttt cggattctat tttcaaaagg
     3481 aaccttacta aatcgtctgt tgcggaaata cgcggttatc atgatcggtg taacgatgac
     3541 tgctaccgtt atttttagta tccatacttg ggaacaaaat caaaaacagg cagaaaacat
     3601 gacaagtgat gccgttcaaa gcacaagcag aatgctgaat gataaaacaa ccttgagtag
     3661 gatcatcaaa aaccagcttg tagggaattc cgaaaagatc gaaaatgtga cgacctactt
     3721 aacaaaaccc atagatcagt atctgatgta tgtatacgaa cagcaaaaca gtaccgatga
     3781 attagtttct ttcccaaatc aaatcaaaga tctctacgca aattatgaag aactaagtgc
     3841 gatatatatc gtgttgaatc aactaccgga gtattatgaa tcttctagag aaaacaaagg
     3901 tggggaaata aaaaagggga caccaaaatt actagacgcc ttttatatca agttgcctat
     3961 cacacaagga ggagctgagt caggaagtat ttttattggt tttcaaaaga aagaactaga
     4021 tatgatttta gaaaatctaa cgtcattcaa tggactctct ttatacatga tttctggaac
     4081 gacaaatcga ctatacacat tccatgataa taaaatagct aaagaaacgt ttgaaagaca
     4141 agaaatgatc atcacagaga gtctgaagaa aaattcacaa ttgccgattg acgatctgga
     4201 aaaaaacaat tttgttcaac accaagaact gtctgatgat tatcgtatat tagctgtact
     4261 agataaaaaa agtgtaaaaa tcgaaacggg aaaaaatctt gccccgctga tcattggtat
     4321 cattatttta gacaatatac tgcttatctt tctttataaa acattcaaac gatattcaca
     4381 gcaagtatct atcgtcatgc aggccatgga tcaaacagca gcagggaatt tagagacgcg
     4441 gatcgacata acaaacacgg aatatgaact gaaggaactt tcaatcggta tcaacgaaat
     4501 gttggatagc atcaatcagt ttgtagaaga catctacaaa ttagaaatca aacagcaaga
     4561 cgcacatatg cgggcgcttc aagcacagat caatccacac tttttatata atacatta
//
LOCUS       DACEXQ010000196         4574 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.196, whole genome shotgun sequence.
ACCESSION   DACEXQ010000196 DACEXQ010000000
VERSION     DACEXQ010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.196"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..1181
                     /locus_tag="IXH04_002318"
     CDS             <1..1181
                     /locus_tag="IXH04_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1852886.1"
                     /translation="ADKYEPTVEGEKVEIGGTVDLTDNVTNLPTLPEGTTVTDVTPGG
                     TIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTDLNKEPD
                     ASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVTVTDTRS
                     DADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGTVVV
                     TYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQYH
                     GTADKNGNFTVKVPKLEAGTKVRVTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKT
                     DNQDGNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE
                     "
     gene            1315..2658
                     /locus_tag="IXH04_002319"
     CDS             1315..2658
                     /locus_tag="IXH04_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ1852887.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKLILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDNTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GKEKIEKIVTTRDNKYAMDMVIQAIGFLSHSPIGKKEFLRDVSGAYLTDEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINDQSFTPSGSQFTNGFSIFG
                     YNFYAAGLNLKTARLLSEAIDYIEIEDYIRPIFSKTNNIVKLRISFEKKAKRIVGVQF
                     YSQEDLSGLLTMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNSGA"
     gene            2770..4173
                     /locus_tag="IXH04_002320"
     CDS             2770..4173
                     /locus_tag="IXH04_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ1852888.1"
                     /translation="MKKSLKLASAVLLMSHLLIATPFHVLASENSTYKIAKERTKTTE
                     MSNSDVTAEGSEFSTTSSSESSTTLNAESSTTSSSESSMTSNAESSTTSSSESSTISS
                     TESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFSTSQQIESTETFQSQPV
                     LSGNEEIHFDKNESVENFIMKIGDSARKIGQKYDLYASVMIAQAILESASGQSQLAQA
                     PNYNLFGIKGTYNGSFVIMVTNEDLGNGTLYKTQSKFRVYENYEESFEDYAKLLTKGI
                     SGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNGLIKTYDLTDYDKEVQGP
                     QMNKNGYQIPIHNYSISSLFGNRGDEFHRGLDMAAAQGEPIHAVKAGKVIKAEFHYSW
                     GNYVVIQHEDGSTALYAHQQQYLVKTGDNVVQGQMIGYVGSTGNSTGPHLHLEICKDS
                     SLSQSMLVDPKTLLFGN"
BASE COUNT         1804 a          643 c          845 g         1282 t
ORIGIN      
        1 acgccgataa atatgaacca acagtagaag gcgaaaaagt agaaattgga ggcacagtag
       61 atttaacaga taatgttact aatttgccaa cattaccaga aggaacaaca gtaacagatg
      121 ttactcctgg cggtacaatt gataccaaca caccaggcaa ttacgaaggt gtcattgaag
      181 taacgtatcc agacggtacg aaagatacag tgaaagttcc agtagaagtg acagacaatc
      241 gttctaatgc agataaatac aatcctgaag gacaaaaagt gacgacagac ttaaataaag
      301 agccagatgc atctgaggga attaagaaca aagaagattt accaaaagga actacttatg
      361 cttggaaaga gaaagtagat gttagcacag ctggaaacaa aaaaggtact attgtagtga
      421 catatccaga tggatcatct gatgaagttg aagtagacgt tacagtgaca gatactcgtt
      481 ccgatgcaga taaatacaat cctgaaggac aaaaagtgac gacagactta aataaagagc
      541 cagatgcatc tgagggaatt aagaacaaag aagatttacc aaaaggaact acttatactt
      601 ggaaagaaaa agtagatgtt agtacagctg gaaacaaaaa aggcactgtt gtagtaacat
      661 atccagatgg atctaaagaa gaagtagaag ttactatttc tgtagtagat aaaaaagcac
      721 cgaataaacc tcaagttgat cctattacag agggtgacca aattgttact ggtaaaactg
      781 aaccaaatgc agaggtaaca gttacattac cagacggaag tcaataccat gggacagctg
      841 ataagaatgg taattttaca gttaaagttc ctaaattaga ggcagggaca aaagtgagag
      901 taactgcaac ggatgaatct ggtaatacta gtgaacctac taatgtagtt gtttcttcca
      961 acaaaaaaga cagtggaaaa aacggtagta aaggtagtaa gacggacaat caagatggca
     1021 atagcaatca agacaaaaat agaggcaaaa gccagtcctc aaaagttttt cctaagacgg
     1081 gagagagtga cagtaatatc tttactatta gcggtggttt aatactgtta ggaacgttag
     1141 gattattagg gtataaaaat cgaaaaaaag agaatgaata gaaaatcaat ttcttaagtc
     1201 ttctctgaga agaggaagaa tctttttctt cctctttttg cttttttatg atgggaaata
     1261 agtgttagtt aaagatgaat atacttttta tagaaaggaa aagaatgtaa ttaagtggaa
     1321 aaaatagtaa ttattggttc aaaccattca gggctagccg cagctcgatt tctaaactta
     1381 agcgattcaa attatgaagt tattctaatc gataaaaaat cagaaattag ttacttggaa
     1441 tgtgggacat cgttatttat aaaaggagaa gtaagtaatt tagaagatct tttttatcaa
     1501 aatagaatga cactaaattc tgagataaaa aagttatttc tagagacaga agttgacaaa
     1561 attgatattc aaaataaaaa gattagttgt gtaaaaaaag atggcacaag attttcccaa
     1621 gaatatgaca aacttatatt agctacaggt tcaaagcaaa ttatgttaga tataccgggg
     1681 aataatttag ctaatatttt ttcgataaag aattttccaa atgccaaaga gattttcaaa
     1741 gctttagatg ataatactat taaaaaagta ggtattattg gagcaggata tattggagtt
     1801 gaaattgctg aagctataag aaaaagaggg aaagaagtat atcttttcga tgttgcagat
     1861 agagtgttgt cgacctatta cgaccgtagt ttttcagata aagttgaaga aatactatca
     1921 aaaaacggga tacatctttg tctatcagaa agtcctaaaa agtatttagg aaaagaaaag
     1981 attgaaaaaa tagtgactac aagagataac aaatacgcta tggatatggt catacaagct
     2041 ataggattcc tatctcatag tccaatagga aaaaaggagt tcttgagaga tgttagtgga
     2101 gcatatctaa cggatgaata ccaacaaaca aatataaaag acatatatgc tattggagat
     2161 tgtgctacaa ctttttttac atccattaat agaagtagcg ttgatttttc tatctctaat
     2221 gctctaagaa cggcttatat tgcaactcaa catattaacg atcaaagttt tacaccttcg
     2281 gggtctcaat tcacaaatgg cttttctata tttggttaca atttttatgc agcaggtttg
     2341 aatttaaaaa cagcacgctt attaagcgag gctatagatt atatagaaat agaagattat
     2401 attagaccga ttttttctaa aacaaacaat attgtaaaat taagaattag ttttgaaaaa
     2461 aaagcaaaga gaatagtagg tgtacaattt tatagtcaag aagatctatc tggattattg
     2521 acaatgttct ctcttgctat agaagaacaa attacattag acaaattgaa aatgttagat
     2581 tatttatttc atcctagttt tagtcaaccc tataatttta ttttaaaggc agtaacagga
     2641 ttaaatagtg gagcataatc gttattaaat gaaatcaaga aatatactgt attactcaat
     2701 tcattgaaaa tatttgtcat attttttaat agaaaacact attaaagaaa aaaaagagag
     2761 gtaaatcaaa tgaaaaaatc tttaaaactt gcatcagcag ttctattaat gtcccatctc
     2821 cttatagcaa cacctttcca tgtgttggca agcgaaaatt caacatacaa aattgcaaaa
     2881 gaaagaacca agacgactga aatgagtaat tccgatgtaa cagcagaggg tagtgaattt
     2941 tcaacaactt catcaagtga aagtagtacg actttaaatg ctgagtcttc aacaacttca
     3001 tcaagtgaaa gtagtatgac ttcaaatgcc gagtcttcaa caacttcatc aagtgaaagt
     3061 agtacgattt caagtaccga atcttcaaca acttcaagta gtacaagtga aataacttct
     3121 tcgaaagtta caaagccaaa ccatatagag tcttcaaaaa aggaagcgcc aaaaacggaa
     3181 ggacaaacaa aaactttttc gacatcacag caaatagaat caacagaaac tttccaatca
     3241 caaccagtac tatctggtaa tgaagaaatt cattttgaca aaaatgagtc tgtagaaaat
     3301 tttattatga aaattggtga ttctgcgaga aaaattggtc agaaatatga tttatatgct
     3361 tctgttatga ttgcacaagc aatcttagaa tctgccagtg gtcaaagcca attagctcag
     3421 gcaccgaatt acaatttatt tgggataaaa ggaacttata atggaagctt tgtcataatg
     3481 gtaacaaacg aagatttggg taatggaacg ctttataaga ctcaatcgaa atttagagtg
     3541 tatgaaaact atgaggaaag ttttgaagat tatgccaaat tattaactaa gggaattagt
     3601 ggaaataaag atttttatgc cggagcatta aaagctaata gtaaaacata tcgagaagca
     3661 actaaattcc ttacaggacg gtatgcaaca gatactcaat actatttaaa gctaaatgga
     3721 ttaattaaaa cctatgattt aactgactat gacaaagaag ttcaaggacc acaaatgaat
     3781 aagaacggat atcaaatacc tattcataat tattcaatat ctagtttgtt tggaaatcgt
     3841 ggagatgagt tccatcgagg acttgatatg gcagctgcac aaggtgaacc gattcatgca
     3901 gttaaagctg gtaaagttat taaagcagag tttcactatt cttggggaaa ttacgttgtt
     3961 atacaacacg aagatggatc aacagcgtta tatgctcacc aacaacaata cttagtaaaa
     4021 actggagaca acgttgtgca aggacaaatg attggctatg taggaagtac tgggaatagt
     4081 acaggaccac acctccattt ggaaatttgc aaagatagta gtctttctca aagcatgtta
     4141 gttgatccga aaaccttatt atttgggaac taaaaagaaa aagagcattt tattagaagt
     4201 atggattagg gaatatagta gtagtgggct ctaaagatta tgaaatcttt aagagctctt
     4261 tttaagacga ttgtttaaaa cgaattaacc aagtaggagc tttcctagaa tttataaaat
     4321 tatagtttct tgtatgacat gtatatagca tgctaaaata ccaataaata aggtttaaga
     4381 taaacgcaaa aagtcgaaaa attaacactt attttgtgat attaatttta atttgcaaaa
     4441 caagctatat ggaaaaagtt taaacttttt ctagagtccc tattactagt taaaaagacc
     4501 actgtagtcc tctgataaat catcataaag accacagtag tccccttaaa gtttttccaa
     4561 attaactatc tgag
//
LOCUS       DACEXQ010000197         4572 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.197, whole genome shotgun sequence.
ACCESSION   DACEXQ010000197 DACEXQ010000000
VERSION     DACEXQ010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.197"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(52..978)
                     /locus_tag="IXH04_002321"
     CDS             complement(52..978)
                     /locus_tag="IXH04_002321"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAQ1852889.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(1063..1824)
                     /gene="pflA"
                     /locus_tag="IXH04_002322"
     CDS             complement(1063..1824)
                     /gene="pflA"
                     /locus_tag="IXH04_002322"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAQ1852890.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(2068..4299)
                     /gene="pflB"
                     /locus_tag="IXH04_002323"
     CDS             complement(2068..4299)
                     /gene="pflB"
                     /locus_tag="IXH04_002323"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAQ1852891.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
BASE COUNT         1246 a          925 c          797 g         1604 t
ORIGIN      
        1 ttatatcaat cgcttatcca ttcattttga atgcttcata ctaccatttg attagctaaa
       61 tacagccgtc aattgaggta cgacttgttt tttacgtgaa acgacacctt taagcaaagc
      121 acggttgttg gtcaaagttg tttggaaagc ttcttctact ttttcttttc ctttaccagc
      181 aacaagtaat tctgaatcac tgtctaagat gtttgtgaca actaatacga acagatcgta
      241 gccatttgct ttcatttcat cttccattgc tgcttctagc tctgcttgtc gatccatcac
      301 ttcattaagg tccactgtat tgacttgagc gatacgaacg ttggaactgc ccattgggaa
      361 actttttgca tccaaatcaa gaagaaccgc agctgattta tcacttagat ttgttcctgc
      421 tttcaacatt tcaagtccat aagtgtttag atcgacttca gcgattgctg caagttcttt
      481 tgcagctgct tcatcttcag gcgtacatgt tggtgatttg aacaacaatg tatctgaaat
      541 gatagctgag agcatgattc ctgcgatttc tttaggaatt gttacttggt tttctttata
      601 tagttttaaa ataatcgtac ttgtacatcc aactggttct gcacgatagt acaatggatt
      661 agctgtttca aagtttgcaa tacgatggtg gtctactaca gctaaaatat ttacttcgct
      721 gatatctgaa atactttgtt ggaattcatt atgatccaca agcatgacat ctgcattttc
      781 tgctttagct gactctacta ctcgtggtgc agatagctga aagtggtcta atgcgtattg
      841 tgtttcttca ttaggtgtac ctagagcaac tgcttctgtt tctttaccta attcattttg
      901 caagtgtgaa aacgcaatgg ctgccccgat tgcatctgta tctggatttt ggtgaccaaa
      961 aactaaaatt tttgacatgt cattcgctcc tttgtgattt ctcctattta ataatagcaa
     1021 aaaaacggaa agaagcaata cttatcttct ttccgatgcg acctatcgcg ttaaatattt
     1081 tgtatactct tctgtatgaa gcaatcgttt agcattttcg acacgttctt ttgtaggtgg
     1141 ttcgatccct tctaatggat atttcaaacc aagtgtttcc cacttgtatt ttcccatcgt
     1201 atgataagga agaatctcta ctctgtctac atttgacaag gtttgaataa agttgtctag
     1261 tcggatcaaa tattcatcat aatcacttcg ctcaggaact agtacatggc gaatccaaac
     1321 cggtttgttg atttttgaca aatattgagc catttctaaa atattttcat tgtcccaatg
     1381 cgttagtttt ttatgctctt cattatcgat gtgtttgatg tcaaataaca gtaaatctgt
     1441 atacttcatc aattcttgaa aacgtgaaaa gaaaggttct tcatacgtaa aaggttttcc
     1501 gcatgtatcc aatgtagtat gcatcttccg ctcttttgct tttttaaata gttcgatcaa
     1561 aaaatcgatc tgcaatagag gttctccacc actaacagta ataccacctt tatctcccca
     1621 gtaaggtctg aagcgttctg ctttatctaa aagttcatct gctgtgtatt ctttccctcc
     1681 gccaatattc caagtatctg gattgtgaca aaattggcaa cgcatgcggc atccttgcat
     1741 aaacacgaca aagcgcagtc caggtccgtc tactgaaccg aaagtttcga tagaatgaac
     1801 atagccgatt gttttttctt ccatttaagt tcacatcttt tctttgattt cattttaagt
     1861 acaagttagc tgtcgaagcg aagctttctg aacaaaattt ttcgcaattg ccgcttcgtt
     1921 atgtgtaaaa tttacaggga cggaacatac aagtcctgcc cctgtaaatc gtttctttca
     1981 aataagcata ctgattattc gattttgatg cgatggctat tcaacgtcct ttcgacgcta
     2041 agtcgaaaaa cttttttcac gaacttttta cattctgtcg tgtgaagttc tcatcaagac
     2101 atctaattgt tgttctcttg tcaagtcacg gaatttaact gcataaccag atacccggat
     2161 cgtcaagttc ggatatttca ttggattttc cattgcatca cgcaataaat cattactgaa
     2221 tacattgaca ttcaaatgat aagctccttt tgagaagtat ccatctaaga cattacgcaa
     2281 gttttcgatt cttgtatctt gttcttttcc aagacctgaa ggattgattg tttgggtatt
     2341 tgaaattcca tccaatgcac agccgtaatt cattttagct gttgagttca ggctagctaa
     2401 taacccactt tgttccgcat tgtaacttgg atttgctcct ggcgcaagtg gttttccagc
     2461 ttcacggcca tcaggagtat ttcctgtatt tttaccgtaa acaacatttg aagtaatcgt
     2521 caataatgaa gttgttggtt ttgagttgcg gtaagtttta tgacgtttga tttgtcccat
     2581 gaagtattcc aagatccatg aaactaattc atctgcacgg tcatcatcat ttccgtattt
     2641 agggaagtcg ccatccactt caaaatcaac tgctattcct ttttcattac ggattgcttt
     2701 tacttttgca tatttgatcg ctgacaagct gtctgccgca tgagagatcc cagcaatacc
     2761 tgtagcaaat gtgcgttgca agtcagaatc catcaaagcc agctgagctg cttcataaga
     2821 atatttatca tgcatataat gaatgatgtt caacgtattg acatatagtt ctgctaacca
     2881 atccatcata ttcatgaagt tatgtttcac ttcttcaaag tctagtattt ctgatgtgat
     2941 tggacgatat tctggtccaa cttgggcgcc agtcacttca tcgactccgc cattgatggc
     3001 atataatagt gttttagcca agttagcacg agcgccaaag aattgcatgt cttttccgac
     3061 aactgttcct gatacacagc aagcaatcgc acaatcatca gaaccccatt gttcacgaag
     3121 ctgattatca ttttcgaatt gaatcgaaga actttcgata gcgattctgc tagcatatgt
     3181 tctgaatcct tctggaaggt gttcagaata caaaacagtc aagtttggtt ctggtgaagg
     3241 tcccatattt gttaatgtat gaagcagacg gaaatcattc tttgtaacta atgaacgacc
     3301 atctaatccc atacctgcaa tggataaagt agcccagata gggaatcctg agaataattg
     3361 gttatattca ggtgtacgcg cgaatttgac catacgtaat ttcattacta agtggtcgat
     3421 caattcttgt gcttcttttt ctgtgattac tccattatcc aaatcacgtt ggatatagat
     3481 atcaaggaat gctgagatac gtccgattga catcgcagca ccattttgtg atttgattgc
     3541 tgctaaatat ccgaagtata gccattggac agcttctttt gcatttgcag ctggtttaga
     3601 aatatcatag ccatatttcg cagccatttc tttcatttgc gctaatgcac gatattgttc
     3661 agtgatttct tcacgtaaac gaatcacttc ttcactcatt gtatgataac cagtcatatc
     3721 atgatctttt ttcttttgtt ccatcaaata gtcgattcca tacaatgcca cacgacgata
     3781 gtcaccgatg atacgtccgc gaccgtaagc gtctggaagc cctgtaatga ttttgttttt
     3841 acgagcagca cgcatttcag cagtataagc gtcaaataca ccttgattat gtgttttgcg
     3901 ccagtcagtg aaaatatgtg tcaattcatc gtttggtgta tagccattgc tttttagcgc
     3961 attgttagcc atattgattc cgccgaacgg catgaatgct tgtttcaaag gtttgtctgt
     4021 ctgtagaccg acgatttttt ccaaatcttt atccaaatag cctgcatcgt gtgaagtaat
     4081 agttgatacg atttctgtgt ccatatcata gacaccgtta cgctcgaatt ggatatcatt
     4141 taacttttgc aattcatccc ataatttttc tgttgcttct gttgggcctt ccaaaaagct
     4201 gtcatcaccg cgatattcag tgtagttatt ttgaataaag tctctagtgt ctacgccttc
     4261 tttccatttg gtacctttaa atccattcca ttgctccatt gtatggacct cctaaatttc
     4321 tgttttatgt aacagttttt acgtgattag tataacacag caccctatct ttgcaagggt
     4381 tttcttgttt tttatttaac aatttttatg ttttatttac atatatttaa atcaacgttg
     4441 acataccaac atttaagcat tgtgaacact ttcatctttt tttgcaaacg tataaggatt
     4501 tattaaaaat gaatgaaaaa gagacgccac ctgtattaat acagatggcg tctcttcatt
     4561 aaaatactac aa
//
LOCUS       DACEXQ010000198         4542 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.198, whole genome shotgun sequence.
ACCESSION   DACEXQ010000198 DACEXQ010000000
VERSION     DACEXQ010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4542
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.198"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..913
                     /locus_tag="IXH04_002324"
     CDS             <1..913
                     /locus_tag="IXH04_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HAQ1852892.1"
                     /translation="LLYLCIQINEIMPPHWQEKKKEFKEEIEIKSNDWIEYTWLPLKR
                     NSKRKEFWALQLWPIGKLFIYMASLMVTLIGYLVVVYGVAYYLLSEPKQIAASNIFTA
                     VMTLMCVMCFSIFSYLLVQFIRQNLTMSKLFKSLLDLLILGSMLTTLTDRFEQSEKTV
                     EGIKNFLTTIDQFINGYMYLFEVGFILTLFGCVFIKLIKRSIEKEMVIDLIPTLSGYE
                     YYVDFGKYEELIHTDIVHTSIHEVTVKGIRSTLKDGQETKTIDRRKEVYNFYKVEASL
                     RYGIGKLRLDGSNIQQNKPYVLGKVVI"
     gene            957..1226
                     /locus_tag="IXH04_002325"
     CDS             957..1226
                     /locus_tag="IXH04_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852893.1"
                     /translation="MILLYRIVLIVGTELKILKKEQLFKVKEHHNQLLCIKTGTMNIQ
                     YRTEYLELTNQTDFLSIREQHKQEKLMATYSQLIDEWEQFKESKT"
     gene            1502..1777
                     /locus_tag="IXH04_002326"
     CDS             1502..1777
                     /locus_tag="IXH04_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852894.1"
                     /translation="MEVKIRDIDEKTIQRIDRRCIELSERTGTKWSRNRYLKVLIEND
                     FDHELMDHRKDQFDRLLEKFVDIQTYNTKILEEYIATNNQLIALLIE"
     gene            1819..2241
                     /locus_tag="IXH04_002327"
     CDS             1819..2241
                     /locus_tag="IXH04_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852895.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKPINESVHARVDPFDPTKFLKR"
     gene            2261..3853
                     /locus_tag="IXH04_002328"
     CDS             2261..3853
                     /locus_tag="IXH04_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852896.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEKERRTPDE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPDKRSEWHYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQ
                     ISKSLKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQDSQIKPLIESMD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            3874..4209
                     /locus_tag="IXH04_002329"
     CDS             3874..4209
                     /locus_tag="IXH04_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852897.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLRNEQF
                     DPNQFLVVEYSATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            4355..>4542
                     /locus_tag="IXH04_002330"
     CDS             4355..>4542
                     /locus_tag="IXH04_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852898.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQSGDI
                     LTLNLTTKTFVDAAEDYP"
BASE COUNT         1761 a          654 c          911 g         1216 t
ORIGIN      
        1 gttactttat ttatgtatac agatcaacga aattatgccc cctcattggc aagaaaagaa
       61 gaaagaattt aaggaagaaa tcgaaataaa atccaatgac tggattgaat acacatggct
      121 tcctctgaaa agaaatagca aacgaaagga attttgggca ttgcaattat ggccaatagg
      181 aaaacttttt atttatatgg cttcacttat ggttacttta atcggttatt tggtggtggt
      241 ctatggggtg gcctattact tgttaagtga gccaaaacag atcgcggcgt caaatatttt
      301 cacggcagta atgacattaa tgtgtgtcat gtgcttttct atttttagtt acttactcgt
      361 ccaatttatc aggcaaaatt taacaatgag taaacttttc aaaagtttat tggacttact
      421 tatcttaggc agtatgttaa cgactctaac agatcggttc gaacaatctg aaaaaacggt
      481 agaggggata aagaacttct taacgacgat tgatcaattt ataaatggat atatgtattt
      541 gtttgaagta ggctttattc tcactttgtt tggttgtgta tttataaaac tcattaaaag
      601 atcaatcgaa aaagaaatgg ttattgatct aattccgacg ctgagcggct atgagtatta
      661 cgttgatttt ggaaagtatg aggagttgat ccatacggat atcgttcaca cgagtatcca
      721 tgaagtaacc gttaagggca tcagatctac tttaaaagat ggacaggaga cgaaaacaat
      781 cgaccgaaga aaagaggtct acaattttta taaagttgaa gcaagtctac gatatggcat
      841 tgggaaattg aggttagatg ggagtaatat tcaacaaaac aaaccatatg ttttaggaaa
      901 agtggtgatt tagaacttcc tatgaaagat gaataaataa ggagaatctt ttgaaaatga
      961 ttcttcttta cagaattgtg cttatagttg gcacggagtt aaaaatctta aagaaggagc
     1021 agttattcaa agtgaaagaa catcataatc agttactatg tattaaaacg ggtacgatga
     1081 acattcaata ccgaacagaa tatttagaat taacgaatca aactgatttt ttgtcgattc
     1141 gagagcaaca taagcaagaa aaattaatgg ctacttatag ccaattgatt gatgagtggg
     1201 aacaattcaa ggaatctaaa acataaaatt ccatttaaac accttatttt ttaaagagca
     1261 taaggaaacg tgtacccact ttcaaaataa agtgggtaga taggctgtgc ctatcacgtt
     1321 tctgaaaaaa ataaatgacc ccaaaatttt caatttatgt gtaggtttat gggtagcaaa
     1381 aatatgaatg tgggcagcaa aaagttcatt tgtgggtagt aaaattcaaa tgtgtgtagc
     1441 aatgtgtgta gctacacaca ttgctacaca cgctataaca tatagtgaaa aggagatatt
     1501 tatggaagta aaaataagag atattgatga aaaaacgatt caaagaattg accgaagatg
     1561 tattgaatta agtgaacgca ccgggactaa atggagccga aatcgatacc taaaagtttt
     1621 gattgaaaat gattttgacc atgaacttat ggatcataga aaagatcaat ttgatcggtt
     1681 gttagaaaaa tttgtggata ttcaaactta taatacgaaa attttagaag aatatattgc
     1741 aacaaataat caattaatcg ccttgttaat tgaataataa aaatagtatt gtgaatttat
     1801 aaaaggagga aatcaatttt ggcaaaagca acaagaaaac atgtatatct ttacgatatg
     1861 catatcgaag tacttgatga aataatttct actcatgaag atatatctaa ttatagtgaa
     1921 gcagtcagac atttgtgtat gatgtatgat ccaaaaatcg aagaaaaaat aaatccaatt
     1981 caaattaaat tgaatgcaat gggaaaagaa atttctatat taaccgaaat ggtttcagag
     2041 tttgcggatc ttcatttgaa agatacagga atactaattg gtacaaattc aactgtctat
     2101 caagaagcaa aagagcgtgt ggaagggaaa ataaaagcga atcaaacgaa aaagtattct
     2161 cctaagatca agaaaccaat aaatgaatct gttcatgcca gagtagaccc gtttgatcca
     2221 acaaagtttt taaaaagata aaaagtagaa ggggaacatg atgaataaat cgcctgcagt
     2281 cattctcact tgtcaattta ccttgcccaa taaaaaatct ttttcaacat acattgatta
     2341 tatgacaaga gaaaaagcgc tggaagaaaa ggaacgacgc acgccggacg aagaacagga
     2401 actgcaacga atcaagaatg ctttagagga tttttatatt cctgaaggag aaactttttc
     2461 ggagagtaag acagataaag aattcagtga aacgtcgaat gaagctcaat cgttgatgga
     2521 acatggaatc aattttgaca agttagagga acaagatttt accaaatatc tttcttatat
     2581 gacccgtcac tatgcgttag aacaaaagaa agaactgacg caaagggaac aaaaagaata
     2641 ccaacggttg aaccaagcaa tgaaaaaata tcaaacagag ccacataaaa aagcttcagg
     2701 aaatcttcct ggagtttttt ctatggcttc agacgaagtg aaaggaagtg atctgaagga
     2761 gatcaaacaa atcttccaaa gagggcaaca aaaaggctca atcatttatc aagatgttgt
     2821 ctctcacgac aattcgtatc ttgaaaaaat gggcttgtac gatcccaaaa cagatacctt
     2881 agatgaagaa ggattaaaag cagctggaag aaaaatgatg gagacattat tcgagaaaga
     2941 acaattgaat gaaacaggtt actggatggc aacgattcac cgtaacacta agcatatcca
     3001 tattcatttt gcagttgttg aaaaagaaaa tacgcgtaaa ccttacacgg aggttgaaaa
     3061 tggtgtgagt tatatcgtac cgaaaggtaa gagaagacaa gcaacattag acgcaatgaa
     3121 gaccaattat gtacatgagt tagaacgttt ttcttatgaa aaggatcgaa tgattggtat
     3181 ggagaaacga aatttattga cgcggaaaag tgatttgcgt aacaccttaa caaaagtggt
     3241 aaaagagcca gcgcgttacg atcgacacgc aatgaactta ttaaatgaag tatataaaac
     3301 cctacctgac aaacgttcgg aatggcatta tggggatgaa aaacgaacca aactttcacc
     3361 atacacaaga gagaaactcg atgagttaac caaatatgtg ttaacaaaag aacctgaata
     3421 tcaagaatat atacagattt caaaatcatt gaaagaagaa gcaaaaagtc tttatgggga
     3481 aagcaagaga gaatcaaagg atgcagaaaa aaatgctctc tttgatttga agaagcgcac
     3541 aggaaatgcc attctaacag aactgaagaa acaggatagt caaataaaac ctttaataga
     3601 aagtatggat cagatgcaat ctatttacaa tgaacgatcc tttcgaacgc ttaaacagtt
     3661 accaacgaat gaatacaaat atagttacga agaattcatg aagcgcagga aacaattcca
     3721 acaaagagtt gtcacgcaac ggagtttgcg tcaattaaat cgaacgatta atgcccatga
     3781 agaaaaatac cacgcaagta aagcctacga agagcaacaa cgaagaattc aaatggaaca
     3841 agaaagacag taaagacagt aggaggaata gcaatgaaac aatatttaga tcaatggaaa
     3901 gttatagaag gatcattaag agaagaacga atcgaacaac ttcccgattg tttagaaaaa
     3961 gagcatttgt ttcagataag agaaatgttg agaaacgaac agtttgatcc aaatcaattt
     4021 ttagtcgttg agtactcagc tactggtgtg tactgttgta atcatgtgaa gggtgaaaaa
     4081 tattttatca ttcaagaata tgagggcaag ttagcgcctt attatactac ttgggaaatg
     4141 aatgaagagg ggatcaataa cttcccgtgt aaaagcattg aagagtctat ttcactcaca
     4201 gaatgttagt tgttaagtga ataaaagaag taaaagagtc ctgaacgtaa ttgtccagga
     4261 ctcttttttt caatgaattg ttttccgcca attcaatgag tggtgcgatt tgcgactaca
     4321 ttatatcaaa agaaagagaa gggaggaaga agaaatggct tattcaacga ttggagttgt
     4381 tgttgaaaaa agcagagaca atctcgtttt tgtgacagaa attcaaacgg gcagagcttt
     4441 tgtcgtcaca gacaaagcgg caaaagcgta tcaaagtggc gatattttaa cgttaaatct
     4501 gactacgaag acgttcgtcg atgcggcaga agattatcca tt
//
LOCUS       DACEXQ010000199         4453 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.199, whole genome shotgun sequence.
ACCESSION   DACEXQ010000199 DACEXQ010000000
VERSION     DACEXQ010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.199"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..534)
                     /locus_tag="IXH04_002331"
     CDS             complement(<1..534)
                     /locus_tag="IXH04_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ1852899.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVF"
     gene            complement(585..4016)
                     /locus_tag="IXH04_002332"
     CDS             complement(585..4016)
                     /locus_tag="IXH04_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAQ1852900.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVKKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASEAEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLSKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDFIVAIGGLTAAFGILLPAILAVSYLFGPMML
                     IIGGIIAVIAGVIVAIKNWGAITDWFSGLWKKFTDWLGDTWESIKDGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQTFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISGIFDNLNISIPHIPLPEFIMEGSF
                     NPLKGQIPHLRVKWHAKGGIFTKPTLLGGMNGVGEAGPEAVLPLKRSVLQEIGDRILS
                     STSVSSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKIQNDNASAWR"
     gene            complement(4033..4215)
                     /locus_tag="IXH04_002333"
     CDS             complement(4033..4215)
                     /locus_tag="IXH04_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852901.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            complement(4218..>4453)
                     /locus_tag="IXH04_002334"
     CDS             complement(4218..>4453)
                     /locus_tag="IXH04_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852902.1"
                     /translation="VGKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMA
                     TASYIYQLMMFKQARDAYQKEFEKANKEKKLQK"
BASE COUNT         1279 a          958 c          681 g         1535 t
ORIGIN      
        1 aaacacataa tcatttaaga tgaactggca tgaagtagct gaaggagtga tgataatctt
       61 tggaaaactt tcttctaaac tattattcag caagttaaat gactgtggtt tatctacggt
      121 tataggtaca tcttcttgaa ctcttgcgaa tggtttcgca gtaatattta catcgaactc
      181 tccccattca acaatatcgt tttctgcatc cccaatatcg atagtctgga taacgtaata
      241 gacgttggga tcgtcagaga attctaattt ctttgcatag tttaaccaat gacgcatgat
      301 ataaaacgat tgcttgaacg cttgatggtc ttccacatcc tctaaatagt tatagtgcaa
      361 tgtaaacgac atatcttcaa acgagtaatc ttgtactaag ccacctaacc tacctaaaac
      421 agaagtttca actctctgtc tttttggaga aggtatggtt ggtctttcag ctaaagccaa
      481 tttatgcaaa taatcaggaa atccatcgat tatagaatgt atacaatcag tcattttttc
      541 acatcctttt taatactaaa aaaacaggag aaatactctc ctgtttaacg ccatgccgaa
      601 gcattatcat tttgaatttt tgtaatgcta tcaatgattt gttgagttgt ttgcttcata
      661 gtaacctcat ctgcgttacc atcaattgtg aaattgaatt cgtagttatt cacaggttga
      721 atcgtttgtg ccctagatga aactgatgtg ctactcaaga tacgatcacc aatttcttgc
      781 aaaacagatc ttttcaaagg taaaactgct tcaggtcctg cttcaccgac accgttcatt
      841 ccacctagta aagttggttt agtaaagata cctcctttag catgccattt tacacgcaaa
      901 tgggggattt gaccttttag cgggttaaag ctgccttcca tgataaattc cggtaacgga
      961 atatgtggta tagaaatatt caaattatca aaaatgccac tgattttgtc tctaatccaa
     1021 tcaattggag cgctaacagt ctttttgatg ccttcccata tgttagcaat tgtactttta
     1081 acattattga atatgtcgga aacaatacct gttagattgg accaaccgct tgaaattgca
     1141 ttttttccat cgtttacttt agagctaata gtgcttgtaa ttccattcca aagattcaaa
     1201 gcagtatttt tgataccgtt ccaaattccg ctaatccacg aagatatact attccaaaca
     1261 ctttgaatgg cacttttagc tgcgtttata gcattgctta tactactagt cacactattc
     1321 cagatatttg atgctgtaga gctgattgaa ttccaaattc cacctaacca actagataca
     1381 gttgaccaaa tattttgaat tactgtagca gctgcttgta ccaagctagt gattgtattc
     1441 ttgatactgt tccaaatact agaagctgtt gcactaattg aattccaaat atttgaagcc
     1501 gtagtactaa tagctgtcca tataccattc caccatgcca ctactggatc aaatatagta
     1561 tggaatgtag ttacaatccc attccaagcg atacttatcc attgtgtcat agtatcccaa
     1621 gtatttttaa ggaaatcaga aataggtgtc caaacagctt gccaagctgc gcccaataac
     1681 tgtccagcta catcaaaaat acccacgata atattaatac cagcttgaat caatgacgtt
     1741 attaatgtcc atggtatttg aacaattcct acaatgtctg cccaaataat cgaccatact
     1801 tctttgactc cgttccaaat atttgaaacc caatcaacga atgtttgcca agtttcttgg
     1861 actccttgcc agatgttgga agctccttca actaatccgc tccatagctc tccaaaccaa
     1921 tcagaaactc cttgccaaat atcttgaacc caatctacaa atccagacca ggtttcttta
     1981 accccatccc aaactgatga ggctccgtct tttatacttt cccaagtatc acccaaccaa
     2041 tcagtaaatt ttttccataa accactaaac cagtcagtaa ttgcacccca gttcttaatt
     2101 gccacaataa cgcctgctat aacagctata attcctccaa ttataagcat catcggacca
     2161 aataaatatg acacggctag tattgccggt aacagtatgc caaatgctgc tgtaagacca
     2221 cctatagcaa caatgaaatc ctgtactggt tgtggaagat tattaaacgc atcagccatc
     2281 tttcctagaa aatcaattac tggttcgagt gcatctatga ttgtgttgcc tataggagct
     2341 aatgaatcct ttaattcagc tattttcccg ttcaactctt gcaacggagt agtagaatct
     2401 tcattcattt tttgtgcaga tccactaaca tcatcaaatg tatggttaac atcagttaaa
     2461 gattggacaa ctttcatcgc attatcttcg ccaagcgcag accaaattgt cgaagcttta
     2521 tttaattggt cgtattgacc atccatattg ctaaaatctt gaatcatgga attaataacg
     2581 tctttttgtg ttcctccgcc atttttccac tcttcaaaag cttttctagt actttcacta
     2641 aacatatcca tgttttgctc aaatcgacca tctgttaacg atattcccat ttccttaact
     2701 aagtcattga ctttatcaag gttataagca cccgcatcta aaccattttg aagcattccg
     2761 aacgtttcat cagctgaata tcccatttga ctccataatt ggctatattc tgccatattg
     2821 tcgcctaatt cgtgcgtttt atctaaaccg ttttgagtac ccgaaaccat taaatccatt
     2881 gcatcttgag cactcaagcc gaaattgacc attaagccat ttacaccacg taacgtttca
     2941 tccatatcag cgcccatggt gttttctagg accatagctt gttccgtgat attttgtaaa
     3001 tcttgattat ttaaatctcc taaattacgc tttaccaaaa tcaatgcatc tgtggactga
     3061 tctaacgatt ctccaaaacc tttataataa atgtctctgg ctacattcgt taattcttca
     3121 gcctcttgtt tagacaaacc aaaattagct tgtatcttac tctgggaact acctacactg
     3181 ttagcagagt ccactgcttg tttccctaat tctgtaagct tatcgccaat gtcgcttaaa
     3241 acgtcagaag cttccattaa attattcata tctattttgc ttccgatatc gtccaagtta
     3301 gttgtatcta catttttagc agcttgtcct aactcttcaa attcacgttc agcatcatta
     3361 agcttcgctt ccatctgcat tgcttctgtg gatgtagcgc caaattcaga ctgtgtagct
     3421 tctaactgtc gtcttaggat atctatcgtt ttctccgcat tttcagattg ttgagaaaca
     3481 tattcttggg ctttcgctaa tttctcggct tcagaagctg attgaccagc agttgcttgc
     3541 cattttttgt attcggattc aatcagagaa gcactagctt gaacattttt ttgttcgcta
     3601 tccaactgtt gcattgtaga ctcgtacgtc tgtatttcgc cttttgcttg agctagtgca
     3661 ttactcgttt tatcaatttc gtttgacaaa cgttgttgcg ctgtttgttg attaatcagt
     3721 tctctttcaa gtttcttaac ttcggtggaa ttttctccat aatatttttt cgcattggct
     3781 aaacgttggc tagttacttc aactttttga ctttgtaatt catactgctt ttctaaagaa
     3841 gataatttac ttcctaactt gtctgattca gaaccagtct gttgtaattg agcttgttct
     3901 agttttaatt ctgctctatt tttagttaat tcagcactga tttcttttaa cgtagatttc
     3961 aatccgtcat cgttagctat gaaagttact tctgcttctg ttctcttttt agccattttt
     4021 tacctccttt ctttagtttt tctgggattg gtttattgca tagtttttcc atccttcata
     4081 agcactcttg ttgtaagcca tttgcaaaat gtcatctaaa cagatatcgc ttaaaaccaa
     4141 atctgaaggc atagaaaaaa cgtcggtcaa catcgaatag acatcgaccc acgtttcaac
     4201 taagagcttt ggaattttta cttttgaagc tttttttcct tatttgcttt ttcaaattct
     4261 ttttgataag catcgcgtgc ttgtttgaac atcatcaatt gataaatata gctggcagta
     4321 gccatatcaa aatcccattt atcgataaat tcatcgaatg aaatataatc agtcatgttc
     4381 gcttggcggt aagcaatata cacagccttt gcaccttgaa taacagaaat atccatggat
     4441 ccttttccca cag
//
LOCUS       DACEXQ010000200         4430 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.200, whole genome shotgun sequence.
ACCESSION   DACEXQ010000200 DACEXQ010000000
VERSION     DACEXQ010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.200"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(543..2048)
                     /locus_tag="IXH04_002335"
     CDS             complement(543..2048)
                     /locus_tag="IXH04_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1852903.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="IXH04_002336"
     CDS             complement(2045..2731)
                     /locus_tag="IXH04_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1852904.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IXH04_002337"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IXH04_002337"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAQ1852905.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1239 a          919 c          729 g         1543 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttcac atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat
//
LOCUS       DACEXQ010000201         4381 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.201, whole genome shotgun sequence.
ACCESSION   DACEXQ010000201 DACEXQ010000000
VERSION     DACEXQ010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.201"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..822
                     /locus_tag="IXH04_002338"
     CDS             <1..822
                     /locus_tag="IXH04_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAQ1852906.1"
                     /translation="HTNMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPV
                     DHAQKMREVLAAAGAGTQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKV
                     EVILPETIEKQVIQAMRSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVE
                     QVKEAFQLDGLRIVQPKNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTA
                     HDMQSAGLIAIDPGHYIESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            837..2069
                     /gene="pepT"
                     /locus_tag="IXH04_002339"
     CDS             837..2069
                     /gene="pepT"
                     /locus_tag="IXH04_002339"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAQ1852907.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            2201..3109
                     /locus_tag="IXH04_002340"
     CDS             2201..3109
                     /locus_tag="IXH04_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAQ1852908.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            3144..3416
                     /locus_tag="IXH04_002341"
     CDS             3144..3416
                     /locus_tag="IXH04_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAQ1852909.1"
                     /translation="MDVLLHNLLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            3427..>4381
                     /locus_tag="IXH04_002342"
     CDS             3427..>4381
                     /locus_tag="IXH04_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAQ1852910.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFL"
BASE COUNT         1385 a          753 c          956 g         1287 t
ORIGIN      
        1 cacacgaaca tggatattat ttgggatgga ctgaatgact ggttttgtga acttctcggt
       61 attgaagtgg agagttattt agtaaagact catgagattc attataaaaa actggccgtt
      121 tatgtacctg tggatcatgc acagaaaatg cgtgaagtat tagctgcagc tggtgctggt
      181 acacaaggtg attataccgg tacaagtttt acctcgattg gacacggtcg atttactccg
      241 gaagctggtg cacaaccagc gattgggaaa gtagggaaga cagaacaagt tcaagaggca
      301 aaagttgagg ttattttgcc ggaaactatc gaaaaacaag tgattcaagc catgcggtca
      361 gctcatcctt atgaggaacc tgcttatgat cttttcgcta tagatgaacc agtcgaaatg
      421 tttgggttag gacgagtagg tgaattgccg caagaaattt ctatagaggc atttgtagaa
      481 caagtaaaag aagcgttcca actagatgga ctgagaatag tccagccaaa aaatgcaaaa
      541 tcctctgtga agcgcattgc gatttgcggt ggaagcggtg aaaaatttta tccgcaagca
      601 atcgcccaaa gagcagatgt ctatatcacg ggagatatct attatcatac ggctcatgat
      661 atgcaaagcg ctgggttgat tgcaattgat cccggtcatt atattgaatc gttatgcaag
      721 caacgattta tcgagaaatt tgaatcttgg aaacaagaag aaaattggga tattaacttt
      781 ttcgtttctg aaacaaatac aaacccattt caatttaatt aaaaggagag aatactatgt
      841 acgaaaatct tttgccacgt tttttacgat atgtcaaaac agaaacacgc tccgatgcaa
      901 caagcaaaac gactccatcg actcagacac aagttgcttt tgcgcaagaa ttgaaaaaag
      961 aattagaaga gttaggctta gctgatgttt tctataatga agataacgga ttcgttatgg
     1021 gaacattacc aagcaatgtt gatcatgaag tgcgttccat tggatttatt tcccacatgg
     1081 atacagcaga ttttaatgca gtaggagtca atccacaaat cattgaaaac tatgatggcg
     1141 agtctacgat cgtactagat gcggaagaac gctatacatt gaatacaaaa gattttccaa
     1201 atttgaaaaa ttatgaaggc cacacattga tcacaacaga tggcagtaca ttactgggag
     1261 cagatgacaa atcagggatt gcggaaatca tgactgcaat ggagatcttg atcaataatc
     1321 cttctatccc tcacggtacc atcaaagtgg cttttggtcc tgatgaagaa atcggtgtcg
     1381 gagcggataa attcgatgtt gagcaattcg atgtagattt tgcttataca gtcgatggtg
     1441 gtcctttagg tgagttgcag tatgaaacgt ttagtgctgc tcaagcaaat attacgatcc
     1501 aagggaaaaa tgtacatcca gggacggcaa aagatacaat gatcaatgct ctacagttag
     1561 caatcgattt tcataatcag cttccagccg atgaagttcc agaaaaaacg gaaggatacg
     1621 aaggtttctt ccatttgatg gcattggatg ggaatcctga agaagcttct atgagttata
     1681 tcattcgcga tcatgatcgt gaaaaattcg aagcacgcaa agcacaaatc acacatatcc
     1741 aagaagagtt gaatcagcga tttgatcaag aacgaatcaa agtagatatg tatgatcaat
     1801 attacaacat gaaagaaatc attgaaaaag atatgagcat cgtggaatta gcgaaacaag
     1861 caatgatcga gcttggtatc tctccagtga tcgaaccagt tcgtggagga acagatggtt
     1921 caaaaatctc ttacatgggt attccgacgc caaatctatt tgccggagga gaaaatatgc
     1981 acggacgatt cgaatttgtt tctttacaag taatggaaaa agcaacagac gtgattgtaa
     2041 aaattgcaga attgaacaca aagagttaaa atcgaaaaag aacagcttat gacaaggcgt
     2101 tcgtcccaat gatggaatgt catctgtcta aggctaaagg gcaggacaat ggaattggtt
     2161 gtcccgccct cttttttgaa gagtaaatag gaggtaaaga atggaaaagc tgctagtttt
     2221 ttataacaaa agttcaggga aagatgaggg tgagaaatta gcacgctggt ttcaagatta
     2281 tgtaaaagaa aagcagccag aattatatgt agaactagct gaaactggcc catcgattga
     2341 tgatgcggaa ttagtcaaga aagcagaagc catagaagct gatactttag taattatagg
     2401 tggagacggc acgattcatc atatcgtcca agcgttccaa aaaacattat ctcaatacca
     2461 tgtcggatta cttccaggtg gaactgtcaa caaccttgca cgtgttcttg agattcccct
     2521 caaaaaagaa gaagctgctg acgtgatcct ggaaggcaat gtacggaaaa tagactatgg
     2581 tcaagtcaat gatgaagtca ttatctctac actgacaatc ggaatcttag cagatacagc
     2641 agtttggatc agtcaagaag aaaaacagcg ttacggctcg tggatcttta tcaagaagtt
     2701 ttttaagtta ttgctgaaga agaaaaaata tcatttggat ttagaaacag acgaggagca
     2761 ttggaaagga aaatcccaac tgttgacagt cacgatgtct aactccgtgg gcggttttac
     2821 aaattttgac gactcagcga ccctagatga tggaaaattt catgtaacga tcgtaccttc
     2881 cttagatatt ttccgattta ttttttattt gcctagaatc atgagaggca aattctatac
     2941 gatccccggc attacttatt tcacagccaa acgcatgaaa atccaagcgc aggaaaaatc
     3001 tgtgggcact cgaacggatg gagacacgac agatgatctt ccagttgaat tatctgttgt
     3061 atcaaaagga ttaacagttt attcaccaaa gaaagaagaa aaagcttaga aatagtagtt
     3121 aacagaaagt tggaagaaga gtaatggatg tattgttaca caatcttttg tggttttacc
     3181 ttttcattgt gaatttttac ttattttgtc tgatggggta tgataaacat caggctagaa
     3241 ctagcggctg gcgtgttcct gagaataatt tgatatttgt cagcctgtta ggcggtggtt
     3301 tgggaggatt gatctctcga aaagttttcc atcataaaac tcggaaaaag aagtttacta
     3361 taggattcgc tgcaggtgta cttgttgatt tgattctcat ttattttttc cattaaggag
     3421 caggagatgt cgtctaaagg aacgaaaact aaaatcattc tgaatctgct tttgctgatt
     3481 gtgatttttg ccattattta ttatttaatc aatcaatcat ttgcagatat ctttgccgaa
     3541 ttgctgtcta ccagtgttgt tgttttggca gcggctgtta tattagggac agtttaccaa
     3601 atcgctgaag ggcgctcaat caaagaaatt gctgaacctt tcgcgccgaa ttttacaacg
     3661 aaagatgggt tcttgacctc tagctatatt gcgttttacc gaatcgtttc gtttggtaca
     3721 ggaacgctga tctcggagat ttatttttat aacaaaaaag gaatgaagct atcccaggga
     3781 gcaggggtga cagcactcca tatgattatg tacaaactgg cagtgatcac ttatgcatta
     3841 gctggcttgg tcatccaatt ctctcttttt tattctaatg gtccatcaat gatctggttt
     3901 atcatcgctg gtatcctttt gacgatcgtg attgttgcct ttttattggc gatgtccgtc
     3961 agcttgaatg ttcaaatatt ttttgtcagc ttatcgaata agttatttaa aagtgagcgt
     4021 ttgcggaaat gggtggatac atgcaatcag cagatctact ctttaaggga aacggtccaa
     4081 accattatcc aagacaggac tgcgctattg cggatttatc tatggaatat gttaaaactt
     4141 gtattttggt atgtacttcc ttatttgatt ctgattgaaa accatcctaa aatagatttt
     4201 ctactgacca tttcgtttat cagctttgca gtagtgcttt caggggttat tcctacgcct
     4261 gctggtattg gttcctttga atttgtctat ttgttattat tcagaccagt tgttggaaca
     4321 gttgacgcag tttcttcgct tttactgtat cgtttcagtt cctttatctt gccatttctc
     4381 t
//
LOCUS       DACEXQ010000202         4379 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.202, whole genome shotgun sequence.
ACCESSION   DACEXQ010000202 DACEXQ010000000
VERSION     DACEXQ010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4379
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.202"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            171..515
                     /locus_tag="IXH04_002343"
     CDS             171..515
                     /locus_tag="IXH04_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAQ1852911.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            516..935
                     /locus_tag="IXH04_002344"
                     /pseudo
     CDS             516..935
                     /locus_tag="IXH04_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(1027..1377)
                     /locus_tag="IXH04_002345"
     CDS             complement(1027..1377)
                     /locus_tag="IXH04_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852912.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1343..2674)
                     /locus_tag="IXH04_002346"
     CDS             complement(1343..2674)
                     /locus_tag="IXH04_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAQ1852913.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVNN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2930..3496
                     /locus_tag="IXH04_002347"
     CDS             2930..3496
                     /locus_tag="IXH04_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1852914.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3641..3728
                     /locus_tag="IXH04_002348"
     tRNA            3641..3728
                     /locus_tag="IXH04_002348"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3673..3675,aa:Ser,seq:cga)
     gene            4062..>4379
                     /locus_tag="IXH04_002349"
     CDS             4062..>4379
                     /locus_tag="IXH04_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_465591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HAQ1852915.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWIL"
BASE COUNT         1594 a          737 c          846 g         1202 t
ORIGIN      
        1 taaaaaaagc gttagcaact atgcttaaaa agcatatcga ctgaataaac taagcattgg
       61 acaggctgaa agactacatt taaaatgaaa tgcgaagaaa caatagaaaa taaagttgcc
      121 ttagaaccat agttgaatca cctaacagtc gtgaagggag aataagagaa atggcagaaa
      181 aacaaaccgc aggacgagat caattaggag attttgcacc aaaatttgca gaattgaatg
      241 atgatgtttt atttggacaa gtatggtcca gagaagaatt atctgctcat cagcgtagta
      301 tcatcaccat atctgcatta atttcaggag gaaattttga acaattgcca gctcatctga
      361 agattggcaa gcaaaatggc attactagtg aagaaatagc agaaatcatt acccatttag
      421 ctttttatgt aggatggcca aaagcttggt cagcttttag tttagcaaaa gaaatctatt
      481 tagaagaaaa tagcgaagat aagaaagagg cgtaaatgac gacaaaacac gaagaaatac
      541 acaatggcgt gatttttcca gcaggtgaaa aaaacgaagc ttacgctcaa gtatttaccg
      601 ggtaaagcta tctgaatatg ttagtgaatg atcccgaagt ttctgttggt gtaggtaatg
      661 taacttttga accaggttgt cgcaatcact ggcatgtcca taaagacggc tatcaaatct
      721 tacttgtgac cggaggagaa ggctggtatc aggaagaagg aaagccagca agacgattaa
      781 tggctggaga tgtcgtcgta acgcacaaag ggattaaaca ttggcatggt gcgacaaaag
      841 acagttggtt tgaacatttg gcgatcactg ctggtaattc agagttttta gaacctgtaa
      901 cagatgaagt gtatgaagga ttggaaaatc aataatgtca agcttgaaca gctcctgaaa
      961 gtagatttta atcgaacttt caggagctgc ttttttgatt cagtctttta taaatagaaa
     1021 atagggttaa taataggaat gagaagcacg tcgaaagatc ttaatattga taatcggctg
     1081 ggcatacatg atttccacgt catcaatcaa actacctaac agatgataat cttcccgatc
     1141 atgtcgggga ttacctaata gttcttgata atagatttcc atactagggt cccgaggatt
     1201 gttcagcaaa tcagcaagta catctaaatc ttctggctga gtttctgccg taccctgtat
     1261 ctctatgaaa agcccttcct cagcaaattc tactccaata cgtatttcag ttatttcatg
     1321 ggaatataaa taagtaagaa gctcatcgat gattcttttt gataattctt tttccaagtg
     1381 tctttttttc ctttctgaca gcggtaaagt tatctagtat cggtacaagg atacctgcaa
     1441 cgaaaccagt tgaaaaacca ctgttatata gatttagtcc tccgtgcaaa gtgctgacgt
     1501 tattgacaag agcaatatgt agtacaccag ctattacacc gtaaaaactg ccgtaaaaac
     1561 cgcttatggg cgcgagacct gtcccaaaca cagctgcaac aagcatgcca gtactggtga
     1621 tctcatgaaa agttccaaac aaagaagcaa taaatattcc gactagtatc ggaaaactgt
     1681 tttttaaatg acaaccaaaa gcactgaaac ccaccgcaga taaaatagca ccgatgacag
     1741 gtccatttaa ctgaccgcca accagcaaaa cataagaaga cagcatcaga ccaactaagc
     1801 ctatgttgat caaagtagca ccaataccag aattagcaat gaaatcagtt gtcaattttc
     1861 cagatgaatc gaaaatttct cgaatcccca aaagccgaaa ggaattataa tagaaaccaa
     1921 ttccgactgt cagcgaaaaa aagccaaaaa taatccaaat gagaggaaag tgatattcat
     1981 tgaaaaccaa tgtattttcg acgattgaat agctgaataa cctcaaaaca gcagtaaaag
     2041 tcatcgcaat caacccagag gtgaatccaa tattataaag tgtgaatcca cgatggaaaa
     2101 ctaaagtttg agcagctaag ggcggaagaa gtagaccaat tgctatgcca gctaaattcc
     2161 ccaataaaat acctatcagt aaaggaaaac gcataccaaa agtgatataa ctaacgaccg
     2221 gtgataatgc gctaccaaac agtccgatca tgatgtactg agaaaaaggt ttattgacaa
     2281 atacagcgta tagatacaca cctaaaatga ttgagataga attataaagg tttttgccaa
     2341 aaaaagagaa accggtaact gttagaatcg aagcaaataa gggaccgttt aaaatcacct
     2401 tgtgccgata agctaaaagc acagaaagta aggtcaaaat ccctacattt atgaatgcgc
     2461 ttccgaaacc gcctaaagca aaataatctg tcaaaagatt gcttggcgag gtaagaatag
     2521 ttagcaatcc actccaatta tcagtaggag tattagaaag caagccaata aaaagaaaaa
     2581 aaacacctgt aaaaccaaga taaaaatact ttcttttctg tgaaacgatt cgtttttgag
     2641 gatgaatata aataacgtca gtttcgtttt ccattacaca ccttcttttt cattttcttt
     2701 aactataact tgaaatgaaa gatttggaca actatcattt aggtcaaaac taaaaatgta
     2761 agggaaccca ttgaatttaa ccaaattttt ttaattttgc aaaaaaaagt taaagaagac
     2821 tgaaaaaaaa tttaaaatat aacgttttga gtaaaatgtt tgcaaaaaat gctaaaaata
     2881 gggtacaata aaacaacata tactgcagta aagaagtggg ggagttttaa tgagaagaaa
     2941 agtctatacc aaggatcaaa ttctaaaagc tgcttatgat gttgttgcaa aggaagggtt
     3001 taaaggattt actgcgagaa atattgctaa aaagatgggg atttcgacac agccgattta
     3061 tctggaattc aaaaacatgg aagatttaaa aaatacgttg ctggattcta tctttgaaca
     3121 actaaaagaa gatgttttcc ctattgaacg tactggtgat aaaatcattg atctcggttt
     3181 gaactatatc tactttgcaa aaaagcatca tagtctttac atggcattgt acattgatga
     3241 acatggggac ggaaagaaaa tgcatgattt ttcatacaat tatttccgtg aaatagttaa
     3301 agaagatgaa agatacaatc aattatcgga tgaatatgtc gatgctctgc atactggtac
     3361 atgggtagca gtgactggct tagcgtcatt gatgtcatca aacatcatgc atccaacaga
     3421 agaacaaatc attgcactga tcaaacggac aattgactcg attttagcat tagataatcc
     3481 tgaaataatc agatagtgtt ctatcagtat gaatttgatc ggtaagtccg ttggctgcaa
     3541 actgtcaatg gacttttttg ctaaaaaaag cgatttctgt tatttatcta ttagaaatag
     3601 gcttgacatt tcgtgaaaaa agtttaaaaa tagtacttac ggaaggttgg cagagtggta
     3661 atgcaccgga ctcgaaatcc ggcgaaccgg ctaataccgg cgcgcaggtt caaatcctgt
     3721 accttcctta tatcaacgat tagaggttgt gaagaagctg tcctgcatca agtaataaga
     3781 acggccaaac aaaaatagct tttcctattt tttcgtttga ttgggcttat taccgcagat
     3841 gcaagctttt gaacaccgtt tgttcggaat aaggggctgt gacaagactt ttgtcacagc
     3901 cccttattgt tgtactttta acctacaata ttttttgatt ccccctgagt actatttttt
     3961 ataaatgcta gtaaatcaat cttctttggc ctttttttga taaaaaaaat actagatgta
     4021 agataaaagt gtaaaaacga tttgttttag gacgggaaac gatgtgtacc gcaatcactt
     4081 atgcaacgaa agaccattat tttggaagga attttgatta tgagatgtct tataatgaag
     4141 tggtcactat tactccaaga aattatcgtt tcgattttag aaaggtaaaa aatttagata
     4201 aacattatgc aatgatagga atagcagctg gggtatccaa ctatccactc tattatgagg
     4261 ctacaaatga aaaagggttg agtatggcag gactgaattt tcctggcaat gctgattata
     4321 aagaattaca agaaggaaaa gataacgttg caccatttga gtttattcca tggatacta
//
LOCUS       DACEXQ010000203         4360 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.203, whole genome shotgun sequence.
ACCESSION   DACEXQ010000203 DACEXQ010000000
VERSION     DACEXQ010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.203"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..377
                     /locus_tag="IXH04_002350"
     CDS             <1..377
                     /locus_tag="IXH04_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1852916.1"
                     /translation="WAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKLVFK
                     DDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILIVSY
                     LIVLVIPFTIYACRHKFQHAEK"
     gene            400..1326
                     /gene="glsA"
                     /locus_tag="IXH04_002351"
     CDS             400..1326
                     /gene="glsA"
                     /locus_tag="IXH04_002351"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAQ1852917.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            1534..2994
                     /locus_tag="IXH04_002352"
                     /pseudo
     CDS             1534..2994
                     /locus_tag="IXH04_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3215..>4360
                     /locus_tag="IXH04_002353"
     CDS             3215..>4360
                     /locus_tag="IXH04_002353"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAQ1852918.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILV"
BASE COUNT         1465 a          703 c          888 g         1304 t
ORIGIN      
        1 tctgggcagc cgtctttaca ttaagcggcg gggggaataa tctttcattt cttgcagcga
       61 tgtcattgac tgttgtaatc tacctgatgt tgtactttat gtttttcgcg ggctatttga
      121 agctggtttt taaagatgac agtcttgaaa gagcctatca ggttccaggc ggaaaagttg
      181 taaaagtaat cgttgcttgt ttaggtcttt tgacttcact ttttgctttt tgtatctcat
      241 ttgttccacc tgctagtatc gcagctggac agcatgggat ttatgaaacg attctgattg
      301 tttcttatct tatcgtctta gttattccat ttacgatcta cgcatgccgt cataaattcc
      361 agcatgcaga aaaataaaaa atgaaagctg gttattcaaa tggaagtaaa agaattagaa
      421 caattgattg gaaaaaattt ccactattat caaacaggac aagtggcgga ttacattcca
      481 gcattagctg aagtggatcc caaacaatta ggtatggcga tttatgattt ggatacaaaa
      541 caactgattg agggaggaga cgccgatgtg cgctttgcca tcgaaagcat gtcaaaagtt
      601 cccgtattgt tattagcaat caaagacaac ggaatagaaa aagtctttca aacaatcaac
      661 acggaaccaa ccggttttgc cttcaattct atcatgaata tggaaatcaa tcatcgcaaa
      721 catccaatga atccttttgt taatgcagga gcgattgcaa caacctcgtt gattgccggt
      781 aaagatgcag atgaaaaatt tgagcgaatc ttgtcattta tgaaagagat ctgtgatgat
      841 cctaatatca cattgaatga agaaatctat cattctgaat caagaacagg tgatatcaat
      901 cgctctcttg cttactatat gaaaggtaat caaatgatcg aaggagacgt accggagata
      961 ctcgatgctt atttcagaca atgctccctc aatgtcaccg ctacaggcat agcaaaatta
     1021 gctgctgtgc tggcaaataa aggaattgct ccttggaatg gcaaacaaat cattactgaa
     1081 gaaagtgcta caatcgtcaa atcaattatg acaactgctg gtttgtatga tgaatcagga
     1141 gagttttccg tccatgttgg tgtacctgca aaaagcggtg taggcggcgg gttgatggca
     1201 gctgtgccaa atcgttacgg cataggtgtc ttcagcccgg cactagatcc atttggtaac
     1261 agcgcagctg gtatccaatt gttgaaagat gtagtcaaag aactagacgc tgacatcttt
     1321 gaataaccat tcttgaaaga ggctggggta aaagtccaaa cggctcaaag ctacctacaa
     1381 tgaggctgaa attgtgagct tgtttgactt ttgtcccagt ttcttttttt attggttttt
     1441 gaaagggctt ttctaaataa aaaggaaaca aaaatacgat ttacagatat gattaaataa
     1501 aatcataaga aaaactggag gcgtgtatca ggaatgctgg aaaattacat cgagcgaaat
     1561 atatttagaa aagtgtatct gtgtgaacaa ttatttgaat tccaagaaat agacatcgaa
     1621 caaacagcta tttctctaag ggtgactaca ccaacgatcc ttcacgatct agaaagtcta
     1681 gccgagtgtc tagaatattg tataaaagaa caagtaagag agaagcataa gtataaactc
     1741 gtattcaaac atggtattgc tctttctgag ctcacacagt ttctgtacgg tcaatcttat
     1801 tttttaaagt ttttaagtta ttattttaat ggtatattca cgagtacaga actggctgat
     1861 cttgaattta tttctttgag taaagtatat acaatcaaaa aaatcgtatt ggattttttt
     1921 aaagaaaatg attatttgaa aaataaagaa atcattatcc cagaatttga ctcgcggaac
     1981 atcttgctag ctttagtacg ttatgtcaat tgggaaggct atgaaaataa aaatccgtat
     2041 gtagaagaag cttgtaacga gttgattgat tatgtagaga ctcatttttt taaaagaaga
     2101 tattcggaag atgaaaagtc ctttatcata cgagaaatag agatcgcaat cggaagaaag
     2161 gcatttcccc ttggactgaa agaagaagat aagaaaagcg cagaaacgaa gccgttattt
     2221 caattagtct gtcaaggatt ggaagagcaa aaaggaattc tagacttaca agaagaagat
     2281 gtctattata ttttttcttt gttcaacagt agaaattata ccaatgagaa catggagctg
     2341 ttaagaaaag atttcgaagt tgtttataaa aattttgttc tcaatgacct ataactgaat
     2401 gaattgattt tacagattat tagtatcgct gaaatcgacg atgcagaaaa ttttattttc
     2461 aagcaatcct ttttacaatt tgtccgtacg ttgtgggcag atggacaagt atttttaccg
     2521 gaaaggatct atcttctatc agaaaaagaa caacttctgt acggaaaaat tgtagaaata
     2581 ttggaagagt ggaaaaagaa aaatcaaatc caactaagat ggaatgaaaa tttcattcga
     2641 aaattcgtca aaagtctttc tttgatcaac agtgaacaag cggggagaca ggaaatagaa
     2701 atttttgttg tttcagaaag ttcagtcaaa caattttttt atcgaaccca atttcatttg
     2761 tatgcggaag aaggcatcgc tgaaataaat cctattttat ttcgttcatt agaagaattg
     2821 cctgatgaat gtctatgtgc gaaaaaaaga gtggttttgt gcgatgctgc tagctatcaa
     2881 aatggattag aaactgaaaa gacaaagatc tatccaatct cattaaaagg tgcaaacatc
     2941 catttgtatc atattgctca ggaacttcac ctattaaaaa aacatatttc ttgatgagac
     3001 cttaattagg agaccttcta caaagctttt taattgtctg tagaaggttt ttatttgagt
     3061 cctgattcgt gcgaagatct acttatataa caagctttct ctgaaatttt tttaaaatgc
     3121 tattgactta gagtcaactc taagtagtag aatagataaa gtgtaacaaa tctattttca
     3181 ttaaaagaag atagtacgga ggagagaatg tattatgagt acagtattac atgtattgga
     3241 tgcaatcaat agccccgcag atgtcaaaaa attatctgtt gaggaaatga aacagttggc
     3301 agaagagatg cgccaaaaaa tcttgcaaaa ggacagcgat gtaggtgggc acgttggtcc
     3361 gaatctaggt gtagtagaat taacgattgc gtttcattat gtatttgatt caccaaagga
     3421 taaaatcgtt tgggatgttt cccatcagtc ttatggtcat aagcttttaa ctggtcgaaa
     3481 aaatggtttt gaaaaaggac attaccgtga agtgagcgga tattccaacc aacatgaaag
     3541 taaacatgac tattttacag tggggcacac atctacatct atcagcttag caacaggttt
     3601 agctaaagcg agagatctga agaaagaatc aggaaatgtg gtagcattta tcggtgacgg
     3661 ctccctaagt ggtggcttag cttttgaagg attgaacaat ggtggtgttt tacaatccaa
     3721 tctaatcgta ttagtaaacg ataatgagat gtctattgat gagaatcatg gcagtttata
     3781 taaaaactta gctgagcttc gtcaaaccaa cggtaaaagc agcaataacc tatttaaagc
     3841 aatgggattt gattatcgtt acgtggataa tggaaacgat ctggaaacgg tgatccgttt
     3901 atttgaagaa gtaaaagata ttgatcatcc gattgtactt cacgtccata cggaaaaagg
     3961 tcgcggttat cagccggcaa tcgataataa aatgaaatat cattggcatg taccatttga
     4021 tctgtccacc ggggaggcaa agcaagtaac ttctggagaa acatttaata tgatcatcat
     4081 ggattatttg gataagaaaa tgcaaacaag tacagctcct attgccgcag tcaatgcagc
     4141 gataccaggc gcctttggac tgaaaacatt ccaagaaaaa catccagatc attattttga
     4201 cgtggggatc gctgaacagc actctatcac tttcgcttct gctttggctt ctcagggagt
     4261 acgtccagtc gtcttccaat caagtacttt cttgcaacgg gcatatgatc aattatcaca
     4321 tgatttagcg atcaacgaga atccagtgat tattttagtc
//
LOCUS       DACEXQ010000204         4328 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.204, whole genome shotgun sequence.
ACCESSION   DACEXQ010000204 DACEXQ010000000
VERSION     DACEXQ010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.204"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            36..1409
                     /gene="tet(L)"
                     /locus_tag="IXH04_002354"
     CDS             36..1409
                     /gene="tet(L)"
                     /locus_tag="IXH04_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAQ1852919.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            1973..3235
                     /locus_tag="IXH04_002355"
     CDS             1973..3235
                     /locus_tag="IXH04_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plasmid recombination enzyme type 2"
                     /protein_id="HAQ1852920.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            3480..>4328
                     /locus_tag="IXH04_002356"
     CDS             3480..>4328
                     /locus_tag="IXH04_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HAQ1852921.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQRFRRMMQYKKINKNLVGFMR
                     ATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPNVKV
                     QMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLISYG
                     GLLKEIH"
BASE COUNT         1503 a          589 c          893 g         1343 t
ORIGIN      
        1 aagatttgaa ttaaagtgta aaggaggaat taattatgaa gtgtaatgaa tgtaacaggg
       61 ttaaatacaa ccaaatttta atttggcttt gcgttttatc tttttttagc gtattaaatg
      121 aaatggtttt gaacgtctca ttacctgata ttgcaaatga ttttaataaa ccacctgcga
      181 gtacaaactg ggtgaacaca gcctttatgt taaccttttc cattggaaca gctgtatatg
      241 gaaagctatc tgatcaatta ggcatcaaaa ggttactcct atttggaatt ataataaatt
      301 gtttcgggtc ggtaattggg tttgttggcc attctttctt ttccttactt attatggctc
      361 gttttattca aggggctggt gcagctgcat ttccagcact cgtaatggtt gtagttgcgc
      421 gctatattcc aaaggaaaat aggggtaaag catttggtct tattggatcg atagtagcca
      481 tgggagaagg agtcggtcca gcgattggtg gaatgatagc ccattatatt cattggtcct
      541 atcttctact cattcctatg ataacaatta tcactgttcc gtttcttatg aaattattaa
      601 agaaagaagt aaggataaaa ggtcattttg atatcaaagg aattatacta atgtctgtag
      661 gcattgtatt ttttatgttg tttacaacat catatagcat ttcttttctt atcgttagcg
      721 tgctgtcatt cctgatattt gtaaaacata tcaggaaagt aacagatcct tttgttgatc
      781 ccggattagg gaaaaatata ccttttatga ttggagttct ttgtggggga attatatttg
      841 gaacagtagc agggtttgtc tctatggttc cttatatgat gaaagatgtt caccagctaa
      901 gtactgccga aatcggaagt gtaattattt tccctggaac aatgagtgtc attattttcg
      961 gctacattgg tgggatactt gttgatagaa gaggtccttt atacgtgtta aacatcggag
     1021 ttacatttct ttctgttagc tttttaactg cttcctttct tttagaaaca acatcatggt
     1081 tcatgacaat tataatcgta tttgttttag gtgggctttc gttcaccaaa acagttatat
     1141 caacaattgt ttcaagtagc ttgaaacagc aggaagctgg tgctggaatg agtttgctta
     1201 actttaccag ctttttatca gagggaacag gtattgcaat tgtaggtggt ttattatcca
     1261 tacccttact tgatcaaagg ttgttaccta tggaagttga tcagtcaact tatctgtata
     1321 gtaatttgtt attacttttt tcaggaatca ttgtcattag ttggctggtt accttgaatg
     1381 tatataaaca ttctcaaagg gatttctaaa tcgttaaggg atcaactttg ggagagagtt
     1441 caaaattgat ccttttttta taacaggaat tcaaatcttt ttgttccatt aaagggcgcg
     1501 attgctgaat aaaagatacg agagacctct cttgtatctt ttttattttg agtggttttg
     1561 tccgttacac tagaaaaccg aaagacaata aaaattttat tcttgctgag tctggctttc
     1621 ggtaagctag acaaaacgga caaaataaaa attggcaagg gtttaaaggt ggagattttt
     1681 tgagtgatct tctcaaaaaa tactacctgt cccttgctga tttttaaacg agcacgagag
     1741 caaaaccccc ctttgctgag gtggcagagg gcaggttttt ttgtttcttt tttctcgtaa
     1801 aaaaaagaaa ggtcttaaag gttttatggt tttggtcggc actgccgaca gcctcgcaga
     1861 gcacacactt tatgaatata aagtatagtg tgttatactt tacttggaag tggttgccgg
     1921 aaagagcgaa aatgcctcac atttgtgcca cctaaaaagg agcgatttac atatgagtta
     1981 tgcagtttgt agaatgcaaa aagtgaaatc agctggacta aaaggcatgc aatttcataa
     2041 tcaaagagag cgaaaaagta gaacgaatga tgatattgac catgagcgaa cacgtgaaaa
     2101 ttatgatttg aaaaatgata aaaatattga ttacaacgaa cgtgtcaaag aaattattga
     2161 atcacaaaaa acaggtacaa gaaaaacgag gaaagatgct gttcttgtaa atgagttgct
     2221 agtaacatct gaccgagatt tttttgagca actggatcca ggagaacaaa aacgattttt
     2281 tgaggaaagt tataaattat tttccgaacg atatggcaag caaaatattg cttatgcaac
     2341 agttcataat gatgagcaaa cccctcacat gcatttaggt gttgtgccta tgcgtgatgg
     2401 aaaactgcaa ggaaaaaatg tgtttaatcg tcaagaactg ttatggctac aagataaatt
     2461 ccccgagcat atgaaaaaac agggttttga gttgaagcgt ggtgaacgtg gctctgaccg
     2521 taaacatatt gagacagcta aatttaaaaa acaaactttg gaaaaagaga ttgattttct
     2581 agaaaaaaat ttagcagtta aaaaagatga atggactgct tatagcgata aagttaaatc
     2641 agatttagaa gtaccagcga aacgacacat gaaaagtgtt gaagtgccaa cgggtgaaaa
     2701 gtccatgttt ggtttgggaa aagaaataat gaaaacagaa aagaaaccaa ccaaaaatgt
     2761 tgttatatcg gagcgtgatt ataaaaactt agtgactgct gcgagagata acgataggtt
     2821 aaaacagcat gttagaaatc tcatgagtac tgatatggcg agagaatata aaaaattaag
     2881 taaagaacat gggcaagtta aagaaaaata tagtggtctt gtagagcgat ttaatgaaaa
     2941 tgtaaatgat tataatgagt tgcttgaaga aaacaagtct ttaaagtcta aaataagcga
     3001 tttaaagcgt gatgtgagtt taatctatga aagcactaag gaattcctta aggaacgtac
     3061 agacggctta aaagccttta aaaacgtttt taaggggttt gtagacaagg taaaggataa
     3121 aacagcacaa ttccaagaaa aacacgattt agaacctaaa aagaacgaat ttgaactaac
     3181 tcataaccga gaggtaaaaa aagaacgaag tcgagatcag ggaatgagtt tataaaataa
     3241 aaaaagcacc tgaaaaggtg tctttttttg atggttttga acttgttctt tcttatcttg
     3301 atatatatag aaataacgtc atttttattt tagttgctga aaggtgcgtt gaagtgttgg
     3361 tatgtatgtg ttttaaagta ttgaaaaccc ttaaaattgg ttgcacagaa aaaccccatc
     3421 tgttaaagtt ataagtgacc aaacaaataa ctaaatagat gggggtttct tttaatatta
     3481 tgtgtcctaa tagtagcatt tattcagatg aaaaatcaag ggttttagtg gacaagacaa
     3541 aaagtggaaa agtgagacca tggagagaaa agaaaatcgc taatgttgat tactttgaac
     3601 ttctgcatat tcttgaattt aaaaaggctg aaagagtaaa agattgtgct gaaatattag
     3661 agtataaaca aaatcgtgaa acaggcgaaa gaaagttgta tcgagtgtgg ttttgtaaat
     3721 ccaggctttg tccaatgtgc aactggagga gagcaatgaa acatggcatt cagtcacaaa
     3781 aggttgttgc tgaagttatt aaacaaaagc caacagttcg ttggttgttt ctcacattaa
     3841 cagttaaaaa tgtttatgat ggcgaagaat taaataagag tttgtcagat atggctcaaa
     3901 gatttcgccg aatgatgcaa tataaaaaaa ttaataaaaa tcttgttggt tttatgcgtg
     3961 caacggaagt gacaataaat aataaagata attcttataa tcagcacatg catgtattgg
     4021 tatgtgtgga accaacttat tttaagaata cagaaaacta cgtgaatcaa aaacaatgga
     4081 ttcaattttg gaaaaaggca atgaaattag actatgatcc aaatgtaaaa gttcaaatga
     4141 ttcgaccgaa aaataaatat aaatcggata tacaatcggc aattgacgaa actgcaaaat
     4201 atcctgtaaa ggatacggat tttatgaccg atgatgaaga aaagaatttg aaacgtttgt
     4261 ctgatttgga ggaaggttta caccgtaaaa ggttaatctc ctatggtggt ttgttaaaag
     4321 aaatacat
//
LOCUS       DACEXQ010000205         4272 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.205, whole genome shotgun sequence.
ACCESSION   DACEXQ010000205 DACEXQ010000000
VERSION     DACEXQ010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.205"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..259
                     /locus_tag="IXH04_002357"
     CDS             <1..259
                     /locus_tag="IXH04_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1852922.1"
                     /translation="ISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFM
                     SNITTLGQSYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            269..925
                     /locus_tag="IXH04_002358"
     CDS             269..925
                     /locus_tag="IXH04_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1852923.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            925..2301
                     /locus_tag="IXH04_002359"
     CDS             925..2301
                     /locus_tag="IXH04_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1852924.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2587..4209)
                     /locus_tag="IXH04_002360"
     CDS             complement(2587..4209)
                     /locus_tag="IXH04_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HAQ1852925.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVSQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYSFEEKIP
                     HFSTFGKNYVRQFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANANKHKFT
                     KEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPESGYYVK
                     GEREKQFAYSAHTSCDDNGFILSTIITPGNIHDSQVAFQLVKQSKRLFPEINCVVADA
                     GYKTPKFVHFLTHLNLRPCLPYSRPKGKKGLLSKNEFLYDEYFDCYICPQDQMLAFST
                     VTRERYREYKSNPKECVNCPLLNQCTKSKNHQRVITRHVWGDLMDEVEHLRLTDLNKS
                     IYKKRKQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKE
                     PLFFCSKIRNEVDKKLFQARVTSLEQLLSTVCDVGDISFSIRNQLRFKRSLPVSGNIN
                     VYFTKARFKRLIAITVSSIVMFLWLFRMGVIP"
BASE COUNT         1520 a          636 c          762 g         1354 t
ORIGIN      
        1 catatcgata ccaagtataa tatcttcctt atttttagga aatgtattac taaaagtaat
       61 tgtagatgga ttcattaact cagaggattc aatgatttcc ggtggaagtt taataaataa
      121 tagtagtttt atgtcaaata taacaacact tggacaaagt tatttaatat taataagtat
      181 tattgtttta tcagttgtat ttgcttcttc attaatgtta atcaagaagc ctaaagaaat
      241 cttatcaaaa ataagttagg agaaaataat ggatatatta gaaataaaga atgtagcata
      301 tagttatgca aattctaaag aaaaagtttt gtcaggagta aatcaaaaat ttgaacttgg
      361 gaagttttat gcgatagtag gaaagtcagg aacaggaaaa tctacacttc tttccttact
      421 tgctggactt gataaacctc aaacaggaaa aatattgttt aagaatgaag atatacaaaa
      481 taaaggatat agtaatcata gaaaaaataa tatatcttta gtatttcaaa actataattt
      541 aatagattat ttatcaccaa ttgaaaatat tagattagta aataaatcag cagatgaaag
      601 tatcttgttt gaactaggtt tagataaaaa acaaataaaa agaaatgtta tgaaattatc
      661 tggtggacag caacaaagag tagctattgc tagagcattg gtatcagatg ctccaataat
      721 actagctgat gagcctactg gtaatctaga cagtgttact gctggagaaa taattaatat
      781 attaaagaca ttagctaaag atagaaataa atgtgtaata gttgtaacac atagtaagga
      841 agtagcagat tctgcggata tcattttaga actaagtggt aaaaagttga aaaaagtaaa
      901 taaaatgaat ttggaggttg aataatgata aaaaatgcat ttgcttatgt aactagaaaa
      961 agtttaaaat cattaattat catattagtt attttatcta tgtcaacttt aagtctcatt
     1021 agtttatcta ttaaagatgc tacggacaga gcttcaaaag aaacatttgc taatataaca
     1081 aatagttttt ctatggagat aaatagacaa gtaaatccag gaacacctag aggtggggga
     1141 aatgtaaaag gtgaagatat taaaaagata tctcaaacag atagtataga ctcctatgta
     1201 aaaagaataa acagtgttgc agatttagtt gattatgata ttattgaaac tcaagagact
     1261 cttgcgaatc aatcacctga aagagcaaag aattttaaaa gaacagttat gttaactggg
     1321 gttaacgact caacaaaaga aacaaaattt gtatcagaag catataaatt agtagaagga
     1381 aagcatttag aaaatgaaga taaaaataaa atcttaatgc ataaagattt agctgaaaaa
     1441 aataacctta aagttggaga taagataaaa ataaaatcta atttatttga tgctgataat
     1501 gagaaaggtg cagatgaaac agtagaagta gaaattaaag gtctattcga tgggcataat
     1561 agcggtgtag taagtgcagc acaagaacta tacgaaaata cattaatcac tgatattcat
     1621 acagctgcta aagtttatgg taataccgaa gatacagctg tataccaaga tgcaacattc
     1681 tttgtaaaag gtgataagaa tcttgatagt gtaataaaag atcttgggaa attagatata
     1741 aattggagag aatataattt gattaaaagt tcatcaaatt accctgcatt acaacaatct
     1801 atatcaggta tttactcaat ttcaaataaa ttattttttg gctcattaat atttgcagga
     1861 gttgtagttt cattattatt attcttatgg atgaatgcta gaaagaaaga aatagcagta
     1921 ttactatcat taggtatatc aaaattagaa atttttggtc aattcctaat tgagatggta
     1981 tttatatcaa tccccgcatt cgttggttcc tatttcttag ctcaatatac agctgacaag
     2041 cttgggaata atatattgaa taaggttact ggtgatatag ctaaacaaat agctaaacaa
     2101 tctgcatcta gccaattagg tggtggggca gaagctgaag gatttaacaa gacattatca
     2161 agtttagata tcaatgtatt acctaaattc atgatttatg tagttatatt tatgagttta
     2221 gtacttcttg tatcattgat tatttcttca ttcaatatat taagaaggaa tccaaaagaa
     2281 ttattaattg acaataatta aaattttcgg tgctttttag caccaaaaat gagagtgtaa
     2341 tataaaccgt gtaagtaacc atcaatccta ggcgggtggt tgcttcacgg tttttatttt
     2401 tctaaaataa gatcaagagg tgattacacg atggctagaa agaaaagaaa tcctgatgct
     2461 gagaagttag ctgaatccat tttgaatact tatcaacccg aatctgtaga ggatatgcaa
     2521 gatgttttaa aagatgtgtt tggtccctta tttgaaaaaa tgcttcaagg agaattgaat
     2581 aaccatttag ggtatgacgc ccattctaaa gagccaaaga aacatgacaa tcgacgaaac
     2641 ggttatggca ataagacgct taaaacgagc tttggtaaag taaacattga tgttcccaga
     2701 gaccgggagg cttcttttga acctgagttg attcctaata gaaaacgaga tgtctccgac
     2761 atcgcagact gtagacaaaa gctgttccaa actcgtaact cgagcttgga acagcttttt
     2821 atctacttcg tttcgaattt ttgaacaaaa aaataaaggc tccttgcctt tccatagcca
     2881 ggtggctaat ttctttaaat tcattgcagc aaacactagc atcgtatgcg tcgcgacttt
     2941 ttccaagcct cgatacttgg tccaacgcat cccatgcttt tcttttgcat ccgcaaatat
     3001 tcgttcaatc gtttgtttcc gttttttgta aattgattta ttcagatctg ttaggcgaag
     3061 atgttccact tcatccatca gatctcccca aacatgtctc gtgatgactc gttgatgatt
     3121 tttacttttg gtacattggt ttagcagagg acagttcaca cattcttttg gattggattt
     3181 gtattctcgg tacctttctc gagtaaccgt tgaaaaagcg agcatttgat cttgcggaca
     3241 aatataacaa tcaaaatact cgtcatagag aaactcattt tttgataata gccccttttt
     3301 ccctttgggc cttgaataag gcaaacaagg tcgaaggttc aaatgagtca aaaagtgaac
     3361 aaacttaggt gtcttatacc cagcatcagc gacaacacaa ttgatttcag gaaaaagtct
     3421 cttcgattgt ttgacgagct gaaaggcgac ttgactgtca tgaatatttc caggagtgat
     3481 gattgtgcta agaataaatc cattgtcgtc gcatgaagta tgggcagaat aagcaaattg
     3541 tttttctcgt tcgcctttga cgtagtagcc gctttccgga tcagctttac ttatttttct
     3601 ttctttcagc ttcacttcct tttctaagtc caaagtaaaa gggcgttttc cagcctctat
     3661 tcgttgacga ttgatctcat cttctaattc atcctgatag gctttcgctt catcatgggt
     3721 catttctttc gtaaacttat gtttattggc gttagctttg atgtgggtag aatccagata
     3781 caaattatcc tctgttacaa aacctgcttt aactgcttgt tctaaaatat aggcaaaaat
     3841 atcctcaaac accgtcgttt ctctaaattg gcgaacataa ttctttccaa aagttgagaa
     3901 atgggggatt ttctcctcga aggagtagcc taaaaaccag cggtaagcga cattggtatc
     3961 gacttcctta atggtttgtc gcatggaacg gataccgaat aaatactgaa taaacaccaa
     4021 tttgatcaga atcactggat cgatacttgg gcgacctaaa tcggagtaag tggcttcgac
     4081 gattggataa ataaattcaa aatcaagcgc ggcatcaatc tttcgaacga gatgatcttg
     4141 agaaactaga tcatcaatcg taagcatcgc aaattgattt ctaccattcg tattgttttt
     4201 agacatcata gctatcactc cttgcattcg ttttcataaa tctattataa aagaaaagac
     4261 tgtagacatc at
//
LOCUS       DACEXQ010000206         4256 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.206, whole genome shotgun sequence.
ACCESSION   DACEXQ010000206 DACEXQ010000000
VERSION     DACEXQ010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4256
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.206"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..134)
                     /locus_tag="IXH04_002361"
     CDS             complement(<1..134)
                     /locus_tag="IXH04_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAQ1852926.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     T"
     gene            complement(550..1320)
                     /locus_tag="IXH04_002362"
     CDS             complement(550..1320)
                     /locus_tag="IXH04_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1852927.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(1313..2248)
                     /locus_tag="IXH04_002363"
     CDS             complement(1313..2248)
                     /locus_tag="IXH04_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1852928.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(2842..4236)
                     /locus_tag="IXH04_002364"
     CDS             complement(2842..4236)
                     /locus_tag="IXH04_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ1852929.1"
                     /translation="MNSTYSSVGSEALYQRLRSFDFGEDYQLEKLIAFYQENPQLRER
                     IQYQFARLGKKDHNFAKQYLADGKSKKIGRVGLFIFLGLLPLVGVGLLLLGQVLGIYL
                     AIGGLVFNTVYYLVKKQTLETELNSMSYLVSTISAANQLAKMTTPLQKELKQQTKPLR
                     DIPKLGFSFRMKSGSEAELIFEYVNIMFMLPFISYHFVLTKLEKHSTEAIKLWELLGE
                     LEVAAAVLNYRTYMPITCRPEFTEGGVTAKDIYHPLLKEAVVNPVNWQKNTLVTGSNA
                     SGKSTYVKSIAISCILAQTIQTAIAEQFTLQPGHVLTSMAIEDDLFEGDSYFVSEIKS
                     IKRLLDQVASKERCYCFVDEILKGTNTIERIASSASVVRWLAEYPSLAFVATHDIELT
                     EILKNYCENVHFEEQVTEGKGISFDFKLKEGPSRTRNAIALLKVLNYPKSIVEQAQKE
                     SLLFDEQRQWYPFD"
BASE COUNT         1284 a          923 c          736 g         1313 t
ORIGIN      
        1 gtaaactcag taaagaagac atccggtgct gctgcatgct ttacgacatg gcgaaataca
       61 acatctgtta catcttccat tggtgctaaa acaaaaaaag gctttggcag ctctttccaa
      121 aaattagtag tcatttattt gttactctcc attctatcaa gcagtgaagt cgcaatcttt
      181 ctcaaaaaca ttgtcattat actaggaaac aaaaaaagaa gcaaagtgaa cagcttaaaa
      241 gatgtttctg attgagcaaa aaaaagactg tgacaaaaat cttatcacag tccattttcc
      301 ggaataaatg gtgttcaaaa gcttgcattt gcggtaataa gtccaaccaa acgaaaaata
      361 ggaggagcta tttttgtttg gctgttctta ttacttgatg cagaacagct ttttcacaac
      421 ctctccacta aacggtgttc aatcagctga ccctcggtat tcgctcaaaa aacaacaaaa
      481 tatgaggaac tattttcccg tttgtttctc taatactcag gtcaaaacgc tgatttcaca
      541 acctcttttc taacttctca atcctcttcc tttattgatc aaataccaag taacggcgta
      601 caaaacaacg atgaataaca caagtacgat catcgatgtt gctactggca catcgatcgt
      661 tccaaggaat ccgtaacgga aaccggagat catataaacg atcggattga cttttgaaac
      721 agcttgccaa aagggcggta acatagagat ggcatagaaa acgccaccta gataagttaa
      781 cggctgcaag acaaatgtag gcacgatcga tacatcatca taagactgag caaaaatccc
      841 gttgagcagt cctgctaatg aaaacaagat tgccgtcatc aataatgtaa tgatgaccat
      901 gaaccatgaa tacacatgaa gtggtacaaa gaacaaggaa atgatcgtta cgagagagcc
      961 gacgagaata ctgcgaccca caccaccaat cacaaagccc caaataatca catgcgtagg
     1021 aacaggtgct accaagattt cttcgatatt tttctgaaat ttttgagaga agaaggacga
     1081 agaaacattg gcatacgaac tagtgatagc agacatcata atcaaaccag gaacgatgaa
     1141 ttccatatat gaaaaaccac ccatgtcacc gatccgccca ccgatcattt ttccgaaaat
     1201 aacaaaatac aaggatgtcg tgatcactgg cggaaccagt gtttggaccc atatacgcaa
     1261 atagcggttc gtttccttga tagctaaact ttttaatgca gtaaaataaa gattaaacat
     1321 ttttctcctc cgtatgatta ttttcttctg tgattttcaa gaacagttct tctaaacgat
     1381 tcgacttatt cctcatagaa agaactttga ttccttgtct tgtaagctga tcgaagattc
     1441 cgttcacccc ttgatttcgt tcaacttcga cagcgattgt ctgctcatct tcaaatgtac
     1501 aagcatatcc ttcaatcaca ggtttggtat catatggcgc taaatcaaag ataaatgttt
     1561 caaattgcag ttttgacaga agctctttca tacttgtatt ctcgatcagt tcacccgatt
     1621 ggatgattcc gatgttgcga caaagcattt cagcttcttc tagataatgc gtcgtcaaaa
     1681 tgatcgttgt accattttcg ttcaattctc gtagaaactc ccacatctcg cgtctcaact
     1741 cgatgtcaac tcctgctgtt ggttcatcca agatcagcaa acgtggttca tgcatcaatg
     1801 ccctagcaat catcagtctt cgcttcattc ccccggaaag catacgtgca cggacatttc
     1861 tcttttccca aagattggac tgcttcaaat atttttcgct gcgtttcatc gcttctttgc
     1921 gagagacacc gtagtagcct gcttgattca cgacgatttg ctggaccgtt tcaaaagggt
     1981 taaaattgaa ctcttgtgga accaatccaa tctgttgctt tgctcgaact agatccttgt
     2041 ccagatcata accgaagaca ttcacttttc cagatgtttt attgactaaa gaagtaataa
     2101 tcccgatcgt tgtagacttc ccagctccat ttggccctaa tagcgcatag aaatctcctt
     2161 ctttcaccga aagatcgatt ccccgcaatg cttcgacccc acttccatag atctttttca
     2221 aattatttat ttcaagcgca tttgccattt atttcctatc tccttataga catgattctt
     2281 aattgtatca tggaatacag cagatgaaaa aacaacttgg cttccacaca aaaacacacc
     2341 tgctaacaaa gaggagatgt gttttctagt tcatgatcat cttcgataga ataactatct
     2401 aaacaactct ggaactttta aaatcataaa agtaccgaca gcaactacta cgatgacata
     2461 tagaagatcc ttataactga ggttcttgtc ttttttattc atgttaagct ttcctccgtc
     2521 cttttatcga aagctttttt cttttatttt aaccgaaata aataaaatgg aaatagttat
     2581 tttcaatttc tgttttcgct gtttgtttta cggagaactt tgccatggaa cgtgcgtctc
     2641 gtcgctgatg ctaagttttt tcgataaaag ctgctagaag gaagacagcg tggtttattt
     2701 tgtatcgtct tttgctaaat cttccaagat catctggaca gcagactcgt tcccattgaa
     2761 ttctgccact tttttggcat caatgtactt cttttgataa tcgtcttttt ggacccacgc
     2821 atcatgcttc gtttgataaa gttaatcaaa cggataccac tgacgctgtt catcgaacaa
     2881 caaactttct ttttgggcct gttcaactat ggatttagga taattcaaaa ctttcagcaa
     2941 ggcaatcgca tttcgggtcc ttgaaggtcc ttcttttaat ttgaaatcaa aactgattcc
     3001 ttttccttct gttacttgtt cttcaaagtg gacattctca caataattct ttaaaatctc
     3061 tgtcaattcg atatcatgtg tggcgacaaa tgctaaagat ggatactccg caagccaccg
     3121 aacgactgaa gcggaagaag caatccgttc gatcgtattt gttcctttta aaatctcatc
     3181 tacgaagcag tagcagcgtt ctttggaagc cacttggtcc agcaaacgtt tgatcgattt
     3241 gatttctgaa acaaaataac tgtcgccttc aaataaatca tcttcgattg ccatggaagt
     3301 caacacatgt ccaggttgca gggtaaactg ttctgcaatc gctgtttgga tcgtctgtgc
     3361 tagaatacag ctgatggcta tacttttgac atacgtagat tttcctgatg cattggaacc
     3421 cgtgactaat gtattcttct gccagttgac tggattaact accgcttctt ttaacaatgg
     3481 atgatagata tctttcgctg tcacaccgcc ctcagtaaac tctggtcggc aagtgatcgg
     3541 catataggtg cgataattca aaacggctgc tgcaacttcc aattctccca gcaattccca
     3601 cagcttgatg gcttcagttg aatgcttttc taatttagtg agaacgaaat gataggagat
     3661 aaatggcagc atgaacatga tgttgacata ttcaaaaatc aattctgctt cactcccgct
     3721 cttcattcga aatgaaaaac ctaatttagg gatgtctctt aatggtttag tttgctgttt
     3781 cagctctttt tgaagtggtg tcgtcatttt tgctaactga ttggcggctg aaatcgttga
     3841 aaccaaataa ctcatgctat tcagctctgt ttctagtgtt tgtttcttca ctagataata
     3901 gaccgtattg aatactaagc caccgattgc tagatagatt cctagtactt gccctaaaag
     3961 aagcaaacct acacccacca gcggaagcaa gccaagaaaa ataaataaac ctacgcgtcc
     4021 aatcttttta ctttttccat ccgctagata ttgttttgca aaattatgat ctttttttcc
     4081 taaacgagcg aattgatatt ggattctttc tctcagttga ggattttcct gataaaaagc
     4141 aattagtttt tctaactgat aatcttctcc aaaatcaaag ctacgtaaac gctgatacaa
     4201 cgcttctgaa cctacactag aataggtact gttgatccct tcaaatactt cgaaca
//
LOCUS       DACEXQ010000207         4242 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.207, whole genome shotgun sequence.
ACCESSION   DACEXQ010000207 DACEXQ010000000
VERSION     DACEXQ010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.207"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..321
                     /locus_tag="IXH04_002365"
     CDS             <1..321
                     /locus_tag="IXH04_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1852930.1"
                     /translation="TIGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVKVESSL
                     KFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGILIVLAG
                     IGIL"
     gene            343..852
                     /locus_tag="IXH04_002366"
     CDS             343..852
                     /locus_tag="IXH04_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1852931.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            889..1290
                     /locus_tag="IXH04_002367"
     CDS             889..1290
                     /locus_tag="IXH04_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852932.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            1402..1755
                     /locus_tag="IXH04_002368"
     CDS             1402..1755
                     /locus_tag="IXH04_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852933.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            1789..2232
                     /locus_tag="IXH04_002369"
     CDS             1789..2232
                     /locus_tag="IXH04_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ1852934.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            2249..3334
                     /gene="uxuA"
                     /locus_tag="IXH04_002370"
     CDS             2249..3334
                     /gene="uxuA"
                     /locus_tag="IXH04_002370"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ1852935.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            3327..4040
                     /locus_tag="IXH04_002371"
     CDS             3327..4040
                     /locus_tag="IXH04_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1852936.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
BASE COUNT         1485 a          633 c          954 g         1170 t
ORIGIN      
        1 actattggcc gtaatgctac aggcctaaag caaaacactg gtgttcagac agcgattgaa
       61 atgctatcag ttttagggtt gtttatgatg ggggtgcttg cgggaaacta tgtaaaagta
      121 gaatcttcat tgaaatttgc gatatccgga cgtgagtttg ttatccagga aattcttgat
      181 caaatcgtgc cagggatatt acctttagct gtcgtaatgg gtgtgtactt gttctatacg
      241 aaaaaaggat tgaaagtcac acaagcatta ttatggctga ctggaatatt aattgtgcta
      301 gcaggaatcg gtattttata aaaagaaggg aacgtgaata agatgggtgt agtcaattta
      361 gcgcgtgtgg atgagcgttt gatccatggg caagtaatgg taacgttatc tcaaaaaagt
      421 ggagtgaatt ccatttttgt tgtagacgaa gttgtagcaa aagataaatt tatgagagat
      481 ctttacaaaa atgcaggaag ccgtacagga caaaaaacaa ttgtcattac accagagaaa
      541 gctaaattct attgggatga atatcaattt aaagaataca attgtatttt gatcgcaaaa
      601 acagtccaaa cgatttacga cttagtaaaa cacggaatcc ccatggaaga attgaatatt
      661 ggagggattg ctcaaaaaaa tcctgataca gatcttttag tgacgaaatc tgtttattta
      721 aataaatcag atgctgaaaa attgaaagaa atgaatgaac aatatggcgt aaagaatatt
      781 tattttcaag ccactccatc tgcacctaaa acaggattaa aagatgtatt gaacaagttt
      841 gatttgtcat aaacaaatgc ttagaaaaaa gataaagagg gcgaaaaaat gaagaagaac
      901 gagctgtttc gtgattggga atttcgttat cgctatattt accgtaagcg ccgtacaaaa
      961 aagagtaaac agcgttttct ttcagcatta gtatcagata tttacagtat gagaacagat
     1021 gtgacagtca ttgcttacga tacgctagcc taccgatcaa aaaatattta tgttggagat
     1081 atcgaaaaag cagagaaagt tatctgtact tattacgata cacccgttca tgcattagga
     1141 agttatttca tgtttgattg gaaagaccaa aggaaaaaaa caatctactc tatattattg
     1201 tccttcattt tgttgttcag tttaggttgg tggggaatga tgatatataa caaaaatcct
     1261 catcacgtat tcgatctgct atccgtataa acaggtatca ctgtactagc atttgggagc
     1321 tactttttct tattgggaaa agcagctcgc ggatggccta gcagacaaac ttttattcga
     1381 aatacttcat ccatattaac gatgctagaa atgatccgta cgattgatga tccgagtgta
     1441 gcgtatgcgt ttgttgatga aggctgttat ggagaaaagg gcttagacag cgtgagatcg
     1501 ggtatgaaaa aagaggctat tcttttttat ctagactctg ttggggcaga tacaccgcta
     1561 cagttttcgg gaaattattt ttcaaataaa gaacagtggc tgaaacaagt tgataagttg
     1621 aaagaaaaaa acgtcaatta tatttttagt gcaagaaaaa agcaagcgca gttcttctat
     1681 ttgaccaaaa cagatttacg agggaaaaca tttaattggc aaaatgccaa tcagattatt
     1741 gctctttttc gataagtaaa aagtgcaaaa caaggagtga gaagactaat gttggaaatc
     1801 atgatcgtta cacatggtaa gttgagcgaa ggactgaaag atgcggcgga agtaattgtc
     1861 gggacatcga gccatattga aacaatgtgc ttagagtctg gggaggacat ccaaacattc
     1921 ggagagaagg tcaaagaaaa attaactatg atcaacacag cagaacccat gctagtattt
     1981 gttgatctaa ttagcgcaag tccgtacaat caagcgctct tggcagtcaa tgaactgact
     2041 gaagaaaaga aagcagccac ttatgtcata gggggcgtga atctgccaat gttgttagaa
     2101 gcaatcaatc atcagatgct tcacacaccg atagaagaag cggtggaaca aattgtcaaa
     2161 caaggaaaag ataatttaac tgtttggcat gcttcagcgg aagaaaaaaa attagatgag
     2221 gacgattttt agaaggaagg ctgaaaagat gaagtgggga tttagatggt atggtgaaaa
     2281 aggggattcg atcccattaa aatatgttcg ccaaataccg ggaataaatg ggataattgg
     2341 aactttgctg actaaaatgc ctggagatgt ttgggagatt tcagaaattg aggagttgaa
     2401 agcatctgtt gagagccaag gactggaact gcttggaatt gaaagtgtag cgatccacga
     2461 tgcaatcaag gcagggacag atgaacgaga ttactatatt gataactata tacgaacaat
     2521 caaaaacttg tctcgatgtg gggttcgatt gatttgttat agcttcaaac caatttttgg
     2581 atgggcgaaa actgatttgt ttaatgaaaa caacgatgga agctattctc ttttatacga
     2641 tcagaaaatc gtcgatcaga tggagccagg agaaatgtat caattgatac acagccagtc
     2701 aaagggattc aaattgccgg gttgggaaga agaacggttg aaaaaattcg ctcagctgga
     2761 aaaactgtat gaaggaatga ccgaagaaga attatttgaa aatctgactt attttttaga
     2821 aaaagtgatt ccagtgtgcg aaaaagcaga tgtaaaaatg gctatccatc cagatgatcc
     2881 gccatgggaa ctttttggtt ttcctagagt gacgaaaaat cttggcgact tgaagaaaat
     2941 tctaaatgca gtagattctc catataatgg actaacgctg tgcacaggat ctttaggggc
     3001 aaatccggaa aacgatttag ttgaaatcat ccatgaggta ggttcgcgca tccattttgt
     3061 ccacttcaga aacgtggagt ttttaggaga aagaaagttc aaagaaacgg cacatccctc
     3121 tacagaaggc tcccttgata tgtatcatat tatgaaagcg ttggtgcaag aagggttcga
     3181 cggtcctata cgccctgatc atggtagaac cgtgtggggt gaagtggcta tgcctggata
     3241 tggcctgtat gatcgtgcaa tgggactcac ttacatgcaa ggtctatatg aggcgataag
     3301 taaggaaaaa ccactgggag agcattatga ataaaatcaa gctcttcatc tttgatatgg
     3361 atggcctttt attagaaact ggacgtttag cttatcgtgc ttatgtaaaa tcagcacaaa
     3421 agtatgatta tgaaatgcgt aaggaagtct attatttact aactggccaa acagaaatgg
     3481 cgataagaga acaaatgggc attttgtacg gagaagatgt tccatatatc aaatggcgag
     3541 aagcaatcaa tcaatataaa gaaaaaatcg tgaaagaaga taagagagta tatacgaaaa
     3601 aaggagcaga agaaatttta tcttttgcaa aagaaagagg aatccataca atcgtggctt
     3661 cgtctaatac acgggtaaaa gtagaaatgt atttaaaaat ggaaaatcta tatgccttgt
     3721 ttgatcaaat catttcaggg gatgatgtaa aaaaaggaaa gccggaacca gagatctttt
     3781 tgaaagcttg ttcaaaaatg aatattcctc caagtgaagc acttgtattt gaagactcga
     3841 ttgcagggat cgaagcagca aggcgagcgg gcattttgtc ttttctcatc aaagatcatt
     3901 tgatagggtt gcccgatcat aatgggaagc ataagttgaa agtagatgta agtttatatg
     3961 aatccaataa agcagactat gaattttatg acctgcatca agcaaaacga tttttagaaa
     4021 gtaaagggtt gtatctctaa taaagaggac tattttctag tcctctttat ttattgtcct
     4081 gcacgactga gggaacagat gaaaaagaag taatttttta ttttgaagta tctaccttat
     4141 ttgtagtgaa caatttagaa caaacacaaa acgcatctac tgttatctac cagtagatgc
     4201 gtttaaagag aagaacgaga atcgtccata tgagggtttc ca
//
LOCUS       DACEXQ010000208         4208 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.208, whole genome shotgun sequence.
ACCESSION   DACEXQ010000208 DACEXQ010000000
VERSION     DACEXQ010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4208
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.208"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(436..1749)
                     /locus_tag="IXH04_002372"
     CDS             complement(436..1749)
                     /locus_tag="IXH04_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ1852937.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCILMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLLGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFMGFMWILNVK"
     gene            complement(1994..2218)
                     /locus_tag="IXH04_002373"
     CDS             complement(1994..2218)
                     /locus_tag="IXH04_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852938.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(2238..2606)
                     /locus_tag="IXH04_002374"
     CDS             complement(2238..2606)
                     /locus_tag="IXH04_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ1852939.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGVLT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(2802..3512)
                     /locus_tag="IXH04_002375"
     CDS             complement(2802..3512)
                     /locus_tag="IXH04_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852940.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGDESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(3530..4026)
                     /locus_tag="IXH04_002376"
                     /pseudo
     CDS             complement(3530..4026)
                     /locus_tag="IXH04_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(4063..>4208)
                     /locus_tag="IXH04_002377"
     CDS             complement(4063..>4208)
                     /locus_tag="IXH04_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1852941.1"
                     /translation="ENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNK
                     RIY"
BASE COUNT         1237 a          890 c          683 g         1398 t
ORIGIN      
        1 aaatggatgt ttctattttt tgcttaatca tttattttag tctctctcac taaaaattgt
       61 aacgctttca aatagctatt gcaccttgtt atcggtaaca gaataaaaca aaaaaacagc
      121 tgaggaaatt ccctcaactg ttttttgaaa ggattgattt attcagggaa gaatgctgat
      181 ttcacaactt ctttataaac ggtgttcact cagctgtccc tcggtattag ctcaaaaaac
      241 ggcaaaatat aagaaactat ttttttcgtt tttttctcta atactcaggc cagaacactg
      301 atttcacaac ctctttataa acggtgttca ctcagctgtc ccttggtatt agctcaaaaa
      361 aaggcaaaat atgaggaact attttttcgt ttttttctct aatactcagg ccagaacact
      421 gatttcacaa cctctttatt tcacattcaa tatccacatg aatcccatga atacaaagaa
      481 cagaccatac atgatgggag atacttcttt tcctctttta gctgctaaca tggtaattgg
      541 atagaagata aaacctaatg caatcccatc tgaaatacta taagtcaaag gcatgccgat
      601 caagatcaag aatgaaggga tcgcaatctc taagtttccc cattcgattt gtcgcaaaga
      661 ttgtgccatc aatactccta ccataatcaa tgctggtgca gttacttgtg aagtaacaac
      721 tgatagtaat ggagaaaaga acaagcctaa caagaagaaa atccctgtag taatagctgt
      781 gatacctgaa cgtcctccaa cggcgatacc agctgatgat tctacaaatg caccaacagg
      841 agaagtacct aataaggaac ccgcaagcat agctgttgaa tcagaagcta aagctttccc
      901 tacacgaggc atcttgttgt ctttcatgaa acctgcttgg tttgctaatc caactaaagt
      961 tcctgctgta tcaaagaaag tcacaagcaa gaatgtcagc actacgaccc acatttgaag
     1021 agaattgatg tcccctacat gttttaatgc aactaaaaat gttggtttca aacttggtgc
     1081 tgcggcaaca atttcagaag gcattggaat caaacctgtg accagaccta aaatcgttgt
     1141 agctgccatc ccgatgaaga tgccacctgg tacacgccga acaagcaaga tagcagttac
     1201 gactaatcca aaaatcgtca accaagtaga accaacgttg agtgatccta atcctactaa
     1261 tgtagattca ttcgaaacga tgatcccacc ttcgctcaga ccaaggaaag caataaatag
     1321 accgataccg ccagaaatcg catatttcaa atctgctggg atcgcatcaa tgatcatttc
     1381 ccgcagcttg aagattgtga tcaaaataaa aatcaaagaa gcaacgaata cgccggctaa
     1441 tgctgtttcc catggaatcc ccattcctaa acaaacagaa taagcaaaaa aagcattgat
     1501 cccaagagca ggtgccgttc caattggata acgggctaat acccccatta aaatacagcc
     1561 gatcgcactg gctaatgcag tcgcagtaaa cactgctcct tcatccattc cagaagcacc
     1621 taatacagtt ggatttacaa ataagatata agccattgag ataaatgttg taaatcctgc
     1681 cagtacctca cgttttaccg tggtgttcaa tttttctatt tcgaagtagg aaagtatttt
     1741 ttccttcatc ttccatcccc ctaaatccga acattataaa taaagtccgt taaaatttcg
     1801 atttattaat aaaacaacgt gttttatcat atactaaaac aaaacataag taaacaaaaa
     1861 attcggattt taagcaaaat ccgaacgttc catctgatta aatcccttta tctcaaagtt
     1921 catgaacatt cactttggat tacaagaatt tctttattgt gttaataaag tttcttcatt
     1981 ctagtctcaa aaatcaatag tgtgatttga ttttaccatt tcgtttctgt tcttctttga
     2041 tgaattcttt tgtatcgaca ggattcagat tatttagtcc gccaaaacct gtatcgacca
     2101 tttttcccca gccttttgtc ttcttttttc ctgatttgag attctttgct cgaagcttct
     2161 cactatgttt tgctcgccga tacttcaaat aaccttctaa tcttcccatt agatccactg
     2221 tttctcctcc tttttattca atcagacgac tcagcttttc ctataacaaa ataattgcct
     2281 tcagggtccg aaaaattgaa agtcaagacg ccattgttgt ctgtgatttc tcctggttct
     2341 tccatgttct cgtagatagt ttcaaattca tccgtaaaaa atactaaaga aggcggtggt
     2401 cctaatacct ctggtgaaaa cttctgcaca aatacttttg ggaatacgcc taattctaat
     2461 tcttttgaga tgctgagtac aatactctta aattcttctg gcaattcgat tgtttcagaa
     2521 atttctgcac caattttttc tacaaagaat cggcaaatca tctctacgtc attgacataa
     2581 agcatcacac gtgttttttg tatcatcgtt tgacccttct ttctatttga ttttctttcc
     2641 tgtctataaa ctgattccat tatatcacga aagcgcaaac accttgtgtt tgatactatt
     2701 caagaaaaat aaagcaatag tttctaggat tgctttcctc cacaaacttt ttttacaaaa
     2761 aaaaaacggc aatgaatgcc gctcttctta tttcgattct tttataattc tgcttctgat
     2821 tcatctttgg ttagtgtgtg tctaacacta gttacgccaa atgtttgatc gattttttcc
     2881 ttcaacgctt cgattgcctg tgcgccacgt gccgcttgtt cagcattgat ggattcaatg
     2941 accagcaccg ctgcttgtgt ttcttccgtc agcatcgttc tttgggcttc acgccagttg
     3001 agacagcgac agatcgctcc ttgttcatca tagtagatga tttcttccgg aagtgccgga
     3061 gcatcggaat ctgcacctaa aggcaggaat gattcgtcac cttgggcttt tcctaaatgc
     3121 agatttcctg cgatcttttc gatatcttct ccgccgcaag gtacgccaaa agacaaggaa
     3181 atactattat agatatctac aagcggattg attgggaaaa attcccgttc ttgatttgca
     3241 cgtttcagta aagcttcaat agaagaacgt gcaccttttt tagtcttgaa tgtggtaaat
     3301 gcctgacgcc actgagcaat gacttcgttt tggctgaatg tttcttctgt aaggtaattt
     3361 cttgcttctt ttgctgcctc attcaataat tgatgatacg tttcttcact ttcttttgtt
     3421 acgtggttat cgataccatt tacggataag atttcgatgg ttgcttcagg aaatacttcc
     3481 cagaaattgg tatccactat tacttttttc atctgttcac acctcttgat tagaataatt
     3541 gcttgatgaa tagatagtct gtttggggat catctcccat ataaaaaaca tgttcgctgt
     3601 atttgataaa tcctttttta cgataaaaag cgagcgcaac atcgttgtgt tcccaaacac
     3661 cgagccaaat ggctgttttt tgcttttctt ttgctttttc aatagctgta tctagtaaaa
     3721 atgtaccgat accttgatgt ttaaattttt ttcgtacata gattcgctcg atttctaaaa
     3781 aatgatcagc catttcctca gattgactcg ttcctgtatt tagtttcagg taacccgcag
     3841 gctcttgatc gatactcact aggaaaaaat ctgtttcttt atcttttagc tcttctgtaa
     3901 gtttttctag tgcataagct tgtgtcaagt aatcagctaa atgctccggt aattatcttt
     3961 tccaaatgta tctgtaaatg tttcgatact aatttgttgt agtaagtcta catctgaagg
     4021 cgtcattttt cgaatttcgt atttattcac ggcttcttcc tttcaataga tccttttatt
     4081 gccttttttg acaaaattcc aatcttcgct aacattcttg catactttat ttaaaagata
     4141 ttccaattgt tttgcttctt cgtcagaaag accatttaat gcgactgcat tggagtagtt
     4201 gttttctc
//
LOCUS       DACEXQ010000209         4199 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.209, whole genome shotgun sequence.
ACCESSION   DACEXQ010000209 DACEXQ010000000
VERSION     DACEXQ010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.209"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(2..1222)
                     /locus_tag="IXH04_002378"
     CDS             complement(2..1222)
                     /locus_tag="IXH04_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ1852942.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1242..1847)
                     /locus_tag="IXH04_002379"
     CDS             complement(1242..1847)
                     /locus_tag="IXH04_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005234653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ1852943.1"
                     /translation="MKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELPLF
                     NKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVDKP
                     VMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLIDEE
                     QVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1896..2828)
                     /locus_tag="IXH04_002380"
     CDS             complement(1896..2828)
                     /locus_tag="IXH04_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAQ1852944.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLSNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3195..4109)
                     /locus_tag="IXH04_002381"
     CDS             complement(3195..4109)
                     /locus_tag="IXH04_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1852945.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLINEGKNTYFCYANFEKIHQR"
BASE COUNT         1191 a          849 c          693 g         1466 t
ORIGIN      
        1 tttattttac agaagcactc gaaactgcat ctactgcagt ttcatcgtgt gtgctggcgc
       61 tggttactgc atctgttttt ccaccatgaa cagatgcact tgaaaccgca tcttcatttc
      121 caacaagttg ttgacccgtt tgttgtaaat accactcaat gattggtttg aaatcaagaa
      181 tgtattcatt gattccagca tactctccat tttcatcatg cattgcttgg taattatgca
      241 caatatattt ttctggcact tgttttggta catgaacacg taccgcatct gttgctccgc
      301 ttcgcagttg ttgaattacc cactctacat ttttatacga tttttctggg tgacagttgg
      361 ctaatggatt ccctacctga ccgggcacac gagcagctaa catctctttc gcttcttggc
      421 gcaaattata atataaaaat tggttattat tatctgcaaa agttaattcc atcggcatcg
      481 aatctaaaaa gtaattgatt tgatccactg tcaataaccc acgatttaat ttgacgtaag
      541 tatttccaga aacggcaccg actgcttcag aagctttttc caaccaatga tcatctgaca
      601 tatcaacatc gggtattgtc gtagcaattg ttcctgtcgc atgcaaatct tctggtggaa
      661 gctttttagg ccctgctaag gtagacacga ctttaatgaa ttttacaaaa cttgctaatg
      721 tactgcctaa aaagtcaaca gttcgtggat caatcaagcg atcttcttgg tcaaatgctt
      781 cttttgcttt tcccaataaa aattcatttc cgggcatcac taatgcattc acgcctggtg
      841 cgtccaaaat ttggcgcaag tgtaattgcg cgcgtgaagt tccttgatcg tagtaagaag
      901 cacccacaac cattacaggt ttattttcaa gtggatggat tttaaatgat agccattcaa
      961 gtgcactttt caatcctgct ggaaccgtat gattgtgttc tggcgtagca ataattaccc
     1021 catcagccat taagattttg cgatttagct gttgaatagc aggactattt gtttgatcat
     1081 catcttgatt gaacagaggt aaatctttga tttcaatgat ttctaaatta aatagttcat
     1141 gatattgttt ttggataaat gttaataatt tacggttata tgaaagttca gctgctgatc
     1201 cgacgatacc tattaatttc atctttgttt gctcccctcg attaaatcgt ttcccatgaa
     1261 aagctttcgg cttcacgttt atttgcttgg tgcgcatgtt gcaattgact agtgattgtg
     1321 acaaagagta agaaatcttt gaatagttca cgtaattttg cgacttgttc ttcatcaatg
     1381 agtcgtcctt gctcatcaaa ggcttggagt gaatgaccca ataaaaattc tgagcttggc
     1441 ataattcgtg cctttaactc tggtgaatcc aatatttggc gcaaatgtgc ttgtgcgcgt
     1501 gaagaaccta acgtgccata agacgctccg gtaatcatca ctggtttatc cacaaacgga
     1561 aaaataccat atgaaagcca atttaatgcg ttaatcaatg aagcaggaac cgcatggtca
     1621 tactctggtg tgctgatgat tacgccatct gcttgttcaa tttttccagc aatttcttta
     1681 acaacatttg gtagttctgt gttttcaggt ttgttaaata gtggcagctc cttgatttcg
     1741 acaatttcga tttctgcctc atttacaaaa taatttttca taaactgtaa taattcacgg
     1801 tttgttgacc gcgttgaatt tgttcctact aatccaataa atttcatctt cattctcttt
     1861 cctttctaac atcgatttct gactgttcgc acgtactaaa ttttttcaaa tccttttgta
     1921 tacaagaatt gattctcttt tgttacaact gctccctcga tacccggctg ttcttcaagc
     1981 gctttcaaca cttctaaaga agatgctcca aataaacgtg tagtccagat ttctccatct
     2041 acagaagaat cagacaaaat cgtcaggctt gctacgtcat ttttaactgg aaagccagtt
     2101 ttcggatcaa tgatgtgatg atacactgac gaaccatttt gtagataacg ttcgtagatt
     2161 ccagaggtaa ctaaagattg gttgcttagt ttaaccaccg caagcgactc ttcacgtgaa
     2221 cctttaggat ttcgcaaacc aatacgccaa gcaccatccg aacgtcttgg tgattcacca
     2281 aaaacaacga tatttcctcc taagttaact aaagcaaacg ctgcatttcg ctctcggcaa
     2341 tgtgtaatta atttatctgc aatgtaacct tttgctaacg cccccaagtc aatcatcatt
     2401 cctggtttgg ttaaaaaaac ggttttcatt ttttcatcta attgaatttg gtgtggatcc
     2461 gttagcagaa gtaactcagt tatctctgct tttgttggaa ccaatgcatc ttcaaatcca
     2521 atgtgccatt tttgtacaac cggaccaata gcaatattca gataactatt tcttgcacaa
     2581 ctatgtgctt ttcctaattt gatgagctca aaaagttcag gatgtacaat cacagcttgt
     2641 cgtccagcag cttgattgat ggccattaat tcagaagttg catcatgtgc gctgaagcga
     2701 tgttcataaa cgtgcaacca ttcgcctaac tctgctagta atcgactagg ctcttcatgc
     2761 ccaacttgta atgtaattgt tgtccccatc atttgccaga ttttcgtttc ttcgatcacc
     2821 tgattcattt tgtaccttct ttacttgaac tacggattac atgagtaatt agtttttatt
     2881 tttgttactg ttttcacaac cagatgatac gcctgctaga tttatttgtc aaccgatttt
     2941 atatattttt ttatataata aggtaatttt aatataaaat tttatattgt aaaaaaacaa
     3001 ataagggaaa caaacaacac atttttgtgt gttgcttatt tccctctaac tatcgttttc
     3061 tatttgatgg ttaacctact taaaataaat actgcaacga caaacggaga tcaagcatgc
     3121 tttgtaacgt attttacgag aattacggct ttattttatt actctatctt gctgccatac
     3181 ccgtctatcc ctaatcatct ttggtgaatt ttttcaaagt tagcataaca aaaataagta
     3241 ttttttcctt cgttaatcaa tagtatcatt tttttcatat gatttaattc atcaattaat
     3301 tttttgtttt tattaatttt agcaaaagct agtgcatctt gaatcaattc gagcacttct
     3361 gccttcgact tatttttagc ctgtgcgttc ttcgctaatt gggcagcagc tcgtgctaag
     3421 taataagtca aatggttatc cgaacaaata tcgattgcgt aatttagtag tgcatcactt
     3481 gtttccaaat caccgtcttg cgcaaaaaaa acaccacaat gaaagacgat atttaacacc
     3541 cgccagatat cttctgttgt acgaatcgga tacgtataaa ttttttcaaa cactcgattg
     3601 aaataaactt ctgctttttc caactctttt tctctagcat agaccattcc aatacctgta
     3661 tatgccaata aacgaaacaa cgtgttggct gtttctgcca tcaacaactg atcaaaatgg
     3721 aacaacacat ccgtaattgg tgcattcgta aaaatcattt caaatccttt tagatagtag
     3781 taacgtgcaa tcagctcaga ctcgtcaatt accttgggat caatggctgc taaaatctgt
     3841 gtcaattgtt catattcact cgtaatcagt gaaaactctg ctttttctag ctgttcatca
     3901 agttgtgtat tgatcacgtt catcgaagaa aaaagttcaa ctaacggaag attgattcga
     3961 ttacataatt tgattaaaat ttttaacgaa gggacttgac cattgttctc aaatttactt
     4021 aatgttgctt gtgtacaaat tccttcagac acttcttttt gagataagtt ggcagccttt
     4081 cttgccgtaa taaatttttc aatgttcatt attttctctc caatcagacc tcacacgaaa
     4141 atcatttctg tctcgatttt agcatgtttt ctttgggaat gctttaattg gttaagata
//
LOCUS       DACEXQ010000210         4181 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.210, whole genome shotgun sequence.
ACCESSION   DACEXQ010000210 DACEXQ010000000
VERSION     DACEXQ010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4181
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.210"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..3049
                     /locus_tag="IXH04_002382"
     CDS             <1..3049
                     /locus_tag="IXH04_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ1852946.1"
                     /translation="GTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQP
                     MLEPVKATSEGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYL
                     RIGSLASPKKTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAG
                     SAQTHQLLGTNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQF
                     SDQNKPLSFKVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQ
                     LIRRLEKPEVVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKA
                     KGKIQARVDFVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETG
                     YERPLPSGEELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLD
                     IRFDVTGIEEQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRT
                     EEGTIHVPKNQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDY
                     QKQGFRWLKMLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIY
                     NWQAEVRKFAPSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYL
                     ILDEAQMVKNSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKT
                     KFRELAAEEIAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMR
                     EEITSMDSEAFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKE
                     NKRRVLLFSQFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLI
                     SLKAGGTGLNLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEE
                     RMDSLQQEKRELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(3195..3902)
                     /locus_tag="IXH04_002383"
     CDS             complement(3195..3902)
                     /locus_tag="IXH04_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ1852947.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT         1371 a          748 c          861 g         1201 t
ORIGIN      
        1 tggtacagca aaagagatag attactgcca atgtccctat tgggaggaac ataaatattg
       61 taagcataca gttgctgtgg aactttattt acggtcgaaa aatctatctc gtatcatgaa
      121 aaaagaccag ccaatgctag aaccggtaaa agcaacaagt gagggagaaa tcattgcaaa
      181 aggtttttct cgtctaaaaa cacaaaacaa tcctcaagcg gtgcaacctt tgattattga
      241 ataccaagta gaaacgattg agactaatca gtatcaccca gagctttcta ttttggcact
      301 ttacttgcgt atcggttcat tggcatcacc gaaaaaaaca tatatcgtaa aaaacatcta
      361 tgatttttta caagcttaca cagaaaaaga aacttacact gttaataaac agtaccagtt
      421 tcgtctagat aaacgggcgt ttcaaaaaga agatcaaaaa atactcacgc atcttgcagg
      481 aagtgctcag acgcatcaat tattaggtac gaacggctta caagtcaaag gaaaactaga
      541 taaaagatat ctgcttctcc cagtagaaca agcccgcttc ctgttagagc agatgaatca
      601 gactacacgt ttgcatctgc agataaatga acaaaacgat caaggaatac aattttccga
      661 tcaaaacaaa cctttatcat tcaaagtgga aaaatttgga gaagattatt tgctgcaagc
      721 attgaatgat tttgattttt tctttatgca ttatcagtgg ggagttatcc aagggacatt
      781 ttattctctg acgaaatatc aacaaacggt ttatcaaact tggaaacaat tgattcgccg
      841 attggagaaa ccagaagtag tgttcaagaa aaaagaatta ccgtctttgt ttaaagagat
      901 tatccctctg ctgtcagaga tcggcgacgt agctattgcc cctgcagtag aagaggaagt
      961 agcagatatc cccgtcgaat ttattttctt tttccgaaaa gcaaaaggaa aaatccaagc
     1021 tcgagtcgat ttcgtctatg gcgaagtcat ctattcgact gacccgaagc atgaggtctc
     1081 gacagaacag gcttttcagg tgttacgtga cacagtagaa gagaaacgag tacttgatct
     1141 attcagaatg tatcgctacc agaaaaatga aactggttat gaacgtccat tgccaagcgg
     1201 ggaagaacta tatgcctttt tccgcacaga actatctgtc ttccgtcagt ttggtgaagt
     1261 cagacttgga agaaaattgc gagagttgta tttagatgcg cataaacatc aaccgaaaat
     1321 cgaagtaacc gagaccggtt catggctcga tattcgattc gatgtgacag ggatagagga
     1381 gcaagaaatc gatcatgtcc tgcaaagctt gctgagaaat gatgcttttt atacattaga
     1441 aaatggtcag gttctttctt ttgattcaga agaatttcaa caaacaagcc aagtgttgca
     1501 acaattacgg gaatcgatac gtacagaaga aggaacgatc catgtaccta aaaatcaagg
     1561 attgatcatt caaaatcaat tggaaaaaag caatgctact tttagtgaat cgttccaaac
     1621 gatggtacaa gatctcattc atccagaaag ataccaggca cagcttccca aaggattgaa
     1681 tgcaacgatg agagattatc aaaagcaagg ttttcgatgg ttgaagatgt taggtcatta
     1741 tcagtttggc ggtattttag ccgatgaaat gggacttggg aaaacactac agacaattgc
     1801 ctttttactt tcggaaaaag aagaacggaa aagcttctct gctttgatcg tagcgcctgc
     1861 gagcttaatt tacaactggc aggcagaggt gagaaaattc gccccttctt taagtatcca
     1921 agtaatcaat gggaataaaa aagaacgtga agaattactg gctaaagaca cagatatccg
     1981 agtgacttct tatgccagct taagacagga tctagctaat tatcagtcgc aaaaaattga
     2041 ttatttgatt cttgatgagg cacaaatggt caaaaatagc agtacgaaaa ctgcacaagc
     2101 attgagagaa ttagctgtac ctcagcggtt tgcactgagt gggacaccaa tcgagaataa
     2161 tttagaagaa ttgtggtcat tgtttgcaac gattatgcca ggatttttcc cgactaaaac
     2221 aaaattcagg gaacttgccg cagaagaaat tgcgcaaatg attcgaccgt ttattttacg
     2281 gcgagataaa caaactgtgt tgaaagactt gccggaaaaa acagagatga atctatattc
     2341 tgcacttaca gaagaacaaa aaactgttta tttagcttac ttgcgtcaaa tgcgtgaaga
     2401 aatcacatca atggattctg aagcattcaa gaaaaaccgg atcgggatac ttgctggtct
     2461 gacaagactg cgacaaattt gctgcgatcc tcgtctgttt attgaagatt accaaggcgg
     2521 ttccggcaag ctcgaacaag taaaagatct actggtagct gccaaagaga acaaacgaag
     2581 agttttgctg ttttctcaat ttacagggat gctgactatc ttgcaagaag agttagccga
     2641 gctagggatc tcgacatttt atttaagagg cagtacgaaa ccacaagatc ggttaagtat
     2701 ggtagacgca tttaatgctg gcgaaaaaga tgtttttctg atttccttaa aggctggggg
     2761 aactggattg aatctcacgg gagcagatac ggtcatcttg tatgaccttt ggtggaatcc
     2821 tgccattgaa gaacaagctg ctggacgtgc acatcggatc ggacaaaaaa atgtggtaga
     2881 agtgtggcgt atgatctctg aaggaacgat tgaggaacgc atggattcac tccagcaaga
     2941 aaaacgcgaa ctgttccaaa aagtgatcca aggtaatgaa gaacagttga cgaaactgac
     3001 tgaagaagac atccgcatga ttttaagtgt aggagaaatc gaggagtaag agactcgaaa
     3061 aagcgttcag taatgggtaa gaaagccttt ctaaagaagc gattgtttat ttgatttaaa
     3121 aaatagcaag gaggctggga ttccatttta tcccagcttc cttgctatca tttttttatt
     3181 tctattttta aaggttatac aaagattgtc ttcattgtct ctaacagctg atcaaaagaa
     3241 gaaatctcga tatcgcaaat tggagtctgt tcaattttgc gttctcgatg gttgaaccaa
     3301 agcgcttgcc agttagcact ttttgcacct agaacatcat tgtcgtaatt atcgccaacg
     3361 tataaggtct cttctggaag catatgaaat tctttctcag ctagctgaaa aatctctttt
     3421 tctggttttt ggaaacctgt tgcttgagaa atgagcatat tgcgggaagg aatccaattg
     3481 ttcaattgaa gttgcttcag tttttttgtc tgatggtcag tcggcccatt tgtgataatt
     3541 cctaatggga cattcttttc cttcaagaaa tcgagggttt ttttgacctc ctcatgcaag
     3601 cagatattat caagttcttc ctcgtaaatt ttttgaaata agaggccatc ttcttccgta
     3661 atatgagggt agtctaaatc ttttagtgat tggctgatcc gatgtgctcg catatattct
     3721 agtgtccaat ctcctgccat tacttttggg aagttttcat cactatgatg gcggaaccgg
     3781 atatatagtg gatgcatatc tgcatctgaa acaagcggaa cgactctttt gaccgcgttt
     3841 cgaaatggct gctgctgatc atacatcgta tcatcaacat caaacacaac tgatttaatc
     3901 aaaattttac acgcctttcg gttttcattg attttttcac aaatccttga ctattgtaac
     3961 agaaaaaatt gtctaaaaaa gctgataaat aaaggttttt cttactatat ttctatttcc
     4021 ttgacagaat aaggttttta tatttcagtt tttttcacta aaaatcgtga aacacgtcaa
     4081 taagtttgat gaacaaatta atcattatgg tagaagtcga ttgatttatt ttaatgcaat
     4141 ctctgtggaa ttttcgaaag atttattttt ttaatacagt c
//
LOCUS       DACEXQ010000211         4174 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.211, whole genome shotgun sequence.
ACCESSION   DACEXQ010000211 DACEXQ010000000
VERSION     DACEXQ010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.211"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            78..2075
                     /locus_tag="IXH04_002384"
     CDS             78..2075
                     /locus_tag="IXH04_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852948.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSVEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(2151..2486)
                     /locus_tag="IXH04_002385"
     CDS             complement(2151..2486)
                     /locus_tag="IXH04_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1852949.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            2613..2828
                     /locus_tag="IXH04_002386"
     CDS             2613..2828
                     /locus_tag="IXH04_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1852950.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            2865..3227
                     /locus_tag="IXH04_002387"
     CDS             2865..3227
                     /locus_tag="IXH04_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAQ1852951.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            3273..3599
                     /locus_tag="IXH04_002388"
     CDS             3273..3599
                     /locus_tag="IXH04_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852952.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
BASE COUNT         1492 a          539 c          810 g         1333 t
ORIGIN      
        1 catatagtaa taaagagttt gtttttatta tctgtatgtt ttagtcttta acagaatttt
       61 ataaggagga aacacaaatg gaaacatcaa tgattaatat tccaatttat gtagatcaag
      121 aaaaattatt agagaagcca gttacaaaaa aagaaaaaac tgatgtaaga gataggtgtt
      181 tagataacta tagagaagtt acaagagatg agtttgtaga gatactaaat tcatcacaat
      241 catttattcc aagcaagaca aagaataaag gcaatgacaa ggagagtttt gtggaaacca
      301 gagtaattat tctggatgtc gataacactg ttaaagatga gaaggggaag gtagtagatt
      361 tgagtaaaga tgatagtaga tacttaagcg tagaaaaggt tttatctatc aatttagttc
      421 ataattctgc gtttgcgata caaaaaagta ttcggtactc tgaaaatcta gaaaaataca
      481 agattgtctt tctattaaaa gaagcaatta ctgactatgg taaaatggtt gctgtttacg
      541 aatacttgaa aaagaacatc cccgggtgtg atgacaatgt aaatgcttcg aatagaatgt
      601 tttttggagg atataagtca gatgcagtga taataattaa tgaagacaac atgttagata
      661 ttactgagct tccgattgat tttaccatga catgttctaa aaattgtcaa agtgattcag
      721 ggagagagta tgaactagtc aatgaaaccg attttgttaa gttgataaaa aatgacgaca
      781 aagaagaaat gaaacagtgg tttaaagact gcctgtttga ttcctccgac atgtctttta
      841 atgagattta tgaaagattg ttaaccattg atatgaataa attacttaag tcaagtaaaa
      901 acttaagatg tctgtttcac aacgatcata atccctctgc tagtattttt accagtaaaa
      961 ctgggcattc cattttttat tgtcattcca gtaattgtga tatcagtgaa aattttattg
     1021 gagttgtgat gctaatttta aagaaagaca gtcgggtaga aacatttaat tggctattag
     1081 agaacctaga cttgtatcct acacattttc gaaaattgaa agaggaatca aaagtacttt
     1141 ttgacactct tgaatcaatg aaagacaaac ttttcaggac aataaggaat tatctagagg
     1201 aaatgcgacg tgtctatgat gtcttgcttt ctgatgttaa tttttttgat gatagtaagg
     1261 aaagtgtgac ttgtattttg tcgggggaac aattagctaa aaaaatgagt tcttactatg
     1321 gtaaaactta tgacatagat aaatgtaata agttactatc acttatgact tttataggat
     1381 tgataaaaaa attagatgat gaagatgttc ctttgaagat gctcgaatac ctaaataggt
     1441 taaaggaaga gaaagcgtta ggaaacttta atcataagag aagcaatgtc tatcgattag
     1501 acgtttttaa tgcaacttct gtgattcaaa aattgaatga agatatcctt ccacttctgg
     1561 aaaaaattca ttttacatat caatattttt cttatgaatg ggttaagata tgttttaacg
     1621 aaaaagaggc taaaagagta tttcctcaaa attcccgatc agaactcagc aaggaaaaac
     1681 aattgatttt agatgaagca catgaagttt tacagggctt atcgtggcaa gggacatatc
     1741 taatgacaga gaaagaactt attaacactg tttgtgaacg cctacgtaat gttggggata
     1801 acagcgtgaa aaatgcttta ataaaaaaca gaggctactt tgttaaacaa ggattcactc
     1861 ttgaacgagc ttctaaggat attaagaaac tatacaatgt gaaagcaagt agtagttttt
     1921 taatatattt agcgaaaggg gcagggatag atgaaacaat aaaagaattg atattatcag
     1981 gagctgggat agttcctctg tatcaattta caagtgtaac aagagatgat ggacggagag
     2041 taaggaagag aaaagaccca attgtattaa actagacagg ttatccatag ctcttaatct
     2101 tatttcatct caatacaccc accgttaaag ttcggtgggt tatttatttt tcaatctttc
     2161 aatagctgat tgcgttcatt ttcttgcctt tttaatagct tcatatagtc aattgttcga
     2221 actgcatctt cagtgctaag agaacgatag agagccaaaa actcttcctc ataatctacg
     2281 acaacattgt ctagaaaatg gcttacaggg gttcctaggg ctctagaaag agctgttaag
     2341 ctatcgaggt taggctctgt tacagaattt tcccatcttg ctactgcctg ttgtgtagag
     2401 aagatttttt cagctaattc tttttgtgtc cacccgagag cttttcgctt ttctctaatg
     2461 acctttgcat attgtttctg tttcattaat acacccccat ctcagtatag tacaacaagt
     2521 tgttgtattt gtaatggtat ttgaagtttt ttatatttta tgagtttaaa tacatcatat
     2581 tgttgtatat aataaaacag gaggggagaa tcatgaaaaa agtaagtaat ctcagccgat
     2641 acagaaaaga aaaaggactt tctcaaacgg agttagcaaa aaaaatgaat gttacacagc
     2701 aatgtgtaag ttcgtggcaa acagggagaa cgattcctaa accctatcaa atgaagatgc
     2761 tttctgagat attaagtgta ccaatgaatg agctgttttc tgatgtattt aataaagtaa
     2821 acagttaact aataagcaat tgaatgattt taggaggaga agacatgtat tgtagaaaat
     2881 gtggcaaaga aataacagat gaaagtaagt attgccagtt ttgtggagca ctacaagaaa
     2941 cagatgagac aaccggaaca gacacagtaa agaaaagtac tgagcagaca attaatgaaa
     3001 tgaaggaaca aatcgtacat ggacaagaga cagggaagct gtatttgatt attggttgga
     3061 tttctatggt agtatcgtta gcatttattc ctgttgtatt tggtgctgtt gctgttatta
     3121 tgggctactt atatcgagaa aaagatgaga aaatgggtac gattttgatg atagcagggg
     3181 tagctggagc aatctttggt gtacttttag ggatgtcaat gtactgataa gagggataag
     3241 agcaaattat tttggagata ggagataaaa agttgaataa agtaaatatg aacaagaagt
     3301 ttgtcattat aggtattgtt gtcttggcta ttataggtat tattttttca aaaatgacag
     3361 gtacatctgg attagagaag tcatcaaaac cattggtaac acagatttta caagaacagt
     3421 acaacttaaa tcgatcatgt gatgatgtga cgattactca tgaaaatgga gataacacat
     3481 accgggctaa agctatatta gacaatggaa gtgctatcaa cataaatatt gagtactatc
     3541 caaagaaaga ccgtatatat gtggagatac catatgctga ggtgttaatg ttaaattgat
     3601 ttaaatgtat tatgcatacc gtccaaacag gaactcccaa aggtgtaaat tcactaggga
     3661 gtttttatag tttcctataa attaaatact aatacaggtg acaaaaaaat attacaatga
     3721 ttgtcgggaa tttttatgtt attcactgtt ataacaatat aaaagaaatg aaaaagaaaa
     3781 aattattatg gatatgtttg gtatttttat tgataattgg tggaattgaa atacttacta
     3841 catgtagatt agaaataaat cagtttaaac atttgctagt taatttttaa gaatactagc
     3901 ccaaatcatt ctatcgatac ctttatatct cggaatgatt gaaattggct attgcttact
     3961 gatttttacc aaaaaaacaa gtatcactat tgttgcctat attgttggaa tgctgtttat
     4021 tccatccttt actcaccaaa ttcatcaatt tttcccaaga gcagaattgt tgaagttgtg
     4081 tgatccgtta tatgcatttt tgatgctttc acgattttgg gaatttcccg ctggttatgt
     4141 aggaatggtt ttactggtat agttaatatt agat
//
LOCUS       DACEXQ010000212         4153 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.212, whole genome shotgun sequence.
ACCESSION   DACEXQ010000212 DACEXQ010000000
VERSION     DACEXQ010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.212"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            486..1409
                     /locus_tag="IXH04_002389"
     CDS             486..1409
                     /locus_tag="IXH04_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1852953.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAVSNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            1618..2196
                     /gene="pgsA"
                     /locus_tag="IXH04_002390"
     CDS             1618..2196
                     /gene="pgsA"
                     /locus_tag="IXH04_002390"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAQ1852954.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            2436..3677
                     /locus_tag="IXH04_002391"
     CDS             2436..3677
                     /locus_tag="IXH04_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAQ1852955.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            3786..>4153
                     /locus_tag="IXH04_002392"
     CDS             3786..>4153
                     /locus_tag="IXH04_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination/repair protein RecA"
                     /protein_id="HAQ1852956.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGE"
BASE COUNT         1332 a          760 c          938 g         1123 t
ORIGIN      
        1 atttcggctt attgccgaag aagctacttc tggaacaccg tgtaccaaga ggttgtgaaa
       61 aagctgccct gcatcaagta ataagaacag ccaagtaaaa acagcttctt aatgttttta
      121 acttggttgg acttattacc gcagatgcaa gcttttgaac accgtttaag gaagaggttg
      181 tgaaatcagc gttttgacct gagtattaga gaaacatacg gaaaaatatc tccttatatt
      241 ttgccggttt ttgagctaat accgaggaac agctgattga acaccgttta agaaagaggt
      301 cgtgagcctg ccgtttaact ccgagtcaca aggaacaagt tcaaaaaaca gcttcttata
      361 tttattggat attgtgactt gtgaccgagg agttggcagg agaacaatgt ttattcggaa
      421 aaaaggagct gtgacatgac ttttgtcaca gcacccaaac ataacgtctt tcgttgcaac
      481 agcagatgct tcggagaatc aaacagactg gagaagatga acgagtggct tttgaaacca
      541 tcggagaaaa attacggcaa gcacgcctga ataaaaaaat atctttagat gaattacagc
      601 agattacaaa aattcaaaaa cgttatttgg aagcaatcga caatgatgat tttgaccaat
      661 tgcctggtaa attttacgtg cgggcattta ttcggcaata cgctgaagct gtaggagaag
      721 atggagacca tttagtcgat gtttttgatg ggaaaagcaa attgacagga tcggtccctg
      781 aacgtccgca gcctgaaaca gtaagtactt ctagaaaagc aatgcatcag gaagaatcgc
      841 atccaagtcg cttttggaca agtcttcctg tcattttatt aggattggtt gctttggcga
      901 ttgtcgttgt cgtgggctat atgacttggc aagatcgtca agctgatcca ataatcggag
      961 acacagcttc ttcgcttaaa gtagaaggat cagtcgagga gtcctcttcc tcagagcaaa
     1021 gcacgcagac caaagagtct tctacagtag aatcaacaac aaaatcaagt gaagaaaaga
     1081 aaatggcaat agctacagga gaagattctg gcagtgccat cgcagtaaat atcacagacg
     1141 ctaccaagcc tgtaaccctt gaatttactg ctgtcaatcg tgtatggtta ggtgtgctag
     1201 tagacaatgc ctatgtttat caagggacac tggcagcaaa tgaaacacag agtaccgtgt
     1261 taccggaaac agcaacaaat gcgaccatca cgattggtgc agtaagcaat gcaacgatca
     1321 aagcgaatgg agaatccgta ccagttaatc caggggaaaa caatcagagt ccaaaaaatg
     1381 tgaaccttac tcttcaatac gcagaatgat cgtataaatt agtttacgca tctgtttact
     1441 tgtaggtata aaaaagaagg atcgtgacaa atctgtgtcg cggtccttct tcgttaattc
     1501 aaaaagatca atttcaataa aaatactcct ttgaataaat tcatttggaa aagtaaataa
     1561 tatcggtgga aacttcgctg atagtcacgt agaataggga agaaaggagt cgatagtgtg
     1621 aatttaccaa ataaattaac tgtacttaga atttgtatga tcccgatttt cattttggtt
     1681 gtaggtttac ctttagattg gggacaattt aacgtaggag acaccacact ggctgtcaca
     1741 cagctagtag gtgcagttat ttttgcttta gctagtatca cagactggtt agatggaaaa
     1801 attgcccgtg cgcgaggcct tgtcactaat tttggcaagt ttgcggaccc gttggcagat
     1861 aaaatgctag ttatgactgc ttttatcatg ttggttgacc aaggaaaggc ccctggttgg
     1921 gtagtagcga tcattgtctg tcgtgaatta gccgtcacag gcttgcgttt actacttgtc
     1981 gaacatggag aagtcatggc tgcagcttgg ccgggaaaag tcaagacggc tacacaaatg
     2041 gttgctatta tcttgctgtt tatcaataat atcccattca gcctgatcgg tattccagta
     2101 gatcaaatca tgctttatgc ttgtctgatc tttactatct attcaggtgt ggattacttc
     2161 atcaaaaaca gcggtgtgtt caaaggatca atgtaagtat cgtttagtgg agaggttgtg
     2221 agcctgccgt ctaactctga gtcacaaggg ataatttcaa aaaacagctt ttcatgtttt
     2281 atgtatattg tgacttatga ccgaggtttt ggcaggagaa cactgtttat ttggaacaag
     2341 gagctgtgac aagatttttg tcacagctcc ttgccctctt tttaaataaa aaaatataaa
     2401 aatacagcgt atactatata agggagcggg aagtaatgaa agcagaaata atagctgtcg
     2461 gaacagaact tttactagga caagtcgtga atacgaatgc cactttcttg tcagaacagc
     2521 tagctgatct aggtatcgaa gtttattatc aaacagttgt aggagataat cagcagcgtt
     2581 tagaagagtt gattgctcta gctgaaacaa gaagtgaact catactctta tgtggcggat
     2641 taggaccgac agaagatgac ctgacaaaag aagcaactgc ggctcacctc ggtaagtctc
     2701 tcatacaaaa tacggaagga tacaaaaaat tattggcata ttttgaaaca acccatcgaa
     2761 aaatgaccaa aaacaatctg caacagtccc agattatcga aggcggggtt cctctaccaa
     2821 atcgtacagg gcttgcttta gggacttttt atcaaacaga tacacatgcg tatatccttc
     2881 ttcccggtcc gcctaatgag ttgaaaccaa tgtttgtgga acaagtacgt cctttactag
     2941 aggaacgttt tccttctgaa gaaaaactaa tttctaaagt tttgcgcttt tatgggattg
     3001 gtgaatcgcg actggtcact gagctaaaag atctgattga gacacagatc aatccaacga
     3061 tcgcaccata tgccaaacca aatgaagtga cattacgact gacagtgaaa acaaatgatg
     3121 ttcaggcagg gaatcaagct cttttagcat tagaagaaaa aatccaagaa cgagtagggg
     3181 aatattttta tggatacggc gatgataatt cgctagcaaa agtcgtagtg gaactactga
     3241 aagaaaacaa gcagactgta acagcagcgg aaagtttgac agccggcgcc ttccaagccg
     3301 cattaggcga cattgctggt gtatctgaag tatttccagg cggattcgtt acctactcgc
     3361 tccaaacaaa agctggtttt ctggagattg atccggagct attagcagaa tatggaacag
     3421 tcagcaaaga atgtgtggag cagatggcca ttcaagcacg tgagatagca agaacagatt
     3481 acagtgtagc tttcaccgga gtggctggac cggacacgtt agaaggtcaa tctgcaggaa
     3541 cagtatggat cgctatttca tctaaagacg gcgtggtttc ggaaaaatat cactttgcca
     3601 gagatcgtgc tgctatcaga tataatgcgg ttatgcaagg atttgatctt ttgagaaaac
     3661 agcttttaag aaaatgataa atgcgaatat ttgttcggtt ttttcttgct ttttttcact
     3721 aaaaaagata tgataaggat aatgaaatgt tgctctgatt tgtaaatata aggaggatta
     3781 tcgtattggc agatgatcgt aaagcagcct tagatgctgc actgaaaaaa attgaaaaga
     3841 gctatggtaa aggctctatt atgaaattgg gcgaaaaaat cgaccaacaa atctctacaa
     3901 ttccaagtgg ctctcttgcg ttagatgtcg ctttaggtgt cggcggttac ccccgtggac
     3961 gtatcatcga agtatatgga cctgaaagtt caggtaaaac aacagttgca ctacacgcta
     4021 ttgcagaagt acaaaaaaat ggcggaacgg ccgctttcat tgatgctgag catgcgttag
     4081 atccgcaata tgcacaaaaa ttaggtgtga atatcgatga actacttctt tcacagcctg
     4141 acacaggaga aca
//
LOCUS       DACEXQ010000213         4143 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.213, whole genome shotgun sequence.
ACCESSION   DACEXQ010000213 DACEXQ010000000
VERSION     DACEXQ010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.213"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            85..972
                     /locus_tag="IXH04_002393"
     CDS             85..972
                     /locus_tag="IXH04_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1852957.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTITSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1112..2038
                     /gene="cysK"
                     /locus_tag="IXH04_002394"
     CDS             1112..2038
                     /gene="cysK"
                     /locus_tag="IXH04_002394"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAQ1852958.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2274..2720
                     /locus_tag="IXH04_002395"
     CDS             2274..2720
                     /locus_tag="IXH04_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ1852959.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2993..>4143
                     /locus_tag="IXH04_002396"
     CDS             2993..>4143
                     /locus_tag="IXH04_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ1852960.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGT"
BASE COUNT         1422 a          667 c          890 g         1164 t
ORIGIN      
        1 aattaaaagt ataatttaat taattaagtt tgttttaaga agagaacttt ttattaagtt
       61 tttttatttt gaggaggaat ttgaatgcaa gagctgaaga cccgcagtgc ccggcaccat
      121 agacctaaaa gaaaaatggt gatcaataaa aaagcgattg ctcctttaat tggaacgagt
      181 cttttagttg ggccgatgat ggttacagcg gtaccgcaac atgttcatgc agatgaacaa
      241 ggatctcaaa cagtcgctga tcctcaagcg tttatcgatc aaatcggctg gtctgcgtca
      301 gaagttgctg caagtaatga cttgtatgct tcagtcatga tcgctcaagc gttgttggaa
      361 agtgcatatg gcacttccgg attagctagt gcgccaaatt ataatctatt cggtgtgaaa
      421 ggaagctata acggacaggt cgtttatatg gcaaccaaag aatatgtaaa cggacaatgg
      481 gtaacgatga atgagccatt ccgaaaatat tcttcttatt gggaatcctt ccaagaccat
      541 gcgaatgttc ttcgttcaac cagtttttcc acgggcgatt atcattatgc tggcgtgtgg
      601 aaaagcaata caacaagttt ttatgatgca acagcgtact tgacaggtcg ttatgctacc
      661 gatccaaatt atggggccaa attgaactca ttgattaata cttataattt aacccgtttt
      721 gatacgccat caacaggttc ggctacaaca atcacaagta caacaacaac gactactacg
      781 acgacttcaa cgtcatcatc ttcaacaaca gatgtaaata caaataatgg tcagacatat
      841 acagtcgtgt caggtgatac gctatgggat attgcacaaa aatttggcat gtctgttgat
      901 gatctaatgg cgaaaaatgg catgtctatg tccagttatt ctttattagt tggtcaagaa
      961 ctaaacgttt gatttagata aaaaggcaaa gaaattacca tatatggtat atctttgtct
     1021 tttttttgct agtcaaagac gaatcactcg ccataaatga acagattgta ttatgataag
     1081 taagaagagc aaagacaagg aggaattgaa gatgacagct atctataact cagtcaccga
     1141 attgattggc aaaacgccaa tcgtcaaatt aaataaaatc gtaccagaag attcagcaga
     1201 tgtttttgta aagcttgagt tttttaatcc aggaggaagt gtaaaagatc gtattgcttt
     1261 aagtatgata gaaaaagcag aacacgatgg tctactgaaa ccaggtgata ctatcattga
     1321 acctacttct ggaaatacag gaatcggatt gtcgatggta ggagttgcaa aaggatataa
     1381 agtaatcatc gtgatgcctg agacgatgag tatcgaaaga cgtttattga tgaaagggta
     1441 cggcgcagag ttgattttga ctcctggtgc ggatggtatt tctggatcaa tcagagaagc
     1501 agaacgtttg gcaaaagaaa acggctactt tttaccatta caatttgaaa atgaagcaaa
     1561 tcctcttgtg catgagaaga cgactggtcc tgagatacat caagcatttg gagtaaatgg
     1621 cttggacgcg tttgttgcag gtatcggtac aggaggaacg atcactggcg ctggccgaga
     1681 attaaaacgt gtgtatccga aaatcgaatt aatcggggta gaaccagcag aatctgctat
     1741 tttagaaggg aaagaagcgg gacctcacaa aatccaagga atcggtacag gttttgttcc
     1801 taaaacactg gatacttctg tgtatgataa agtcctttct attagtggag acgaagcgat
     1861 ggaaacagct agagaagttg ggcgaaaaga agggatttta gtaggcatct catctggcgc
     1921 tgcaattgct gccgcgctaa aagtcgcaaa agaattagga aaaggcaaga aggtattagc
     1981 tgtggttccc gacaatggtg aacgttattt atctacagcc ctttaccaag agatataagg
     2041 cacttggttt ttctccagat aggtttatgt aaaaaacttt taaagatgaa aagaacacac
     2101 aaaaatttat gatgagaaaa attatttgaa tagagacgta gaatagtgaa cgcgactgtt
     2161 ttacgtcttt tttcattaaa atacaataaa attgaatttt cttttcatgt taagtggatt
     2221 ttaaaatgta aaaatagtat gataagacga gaataacaac gtggggtgtt tacatggaaa
     2281 ataagttagt cgaagaggtt atcgaaaagt taaaaaaaga gaacattcga atcacacctc
     2341 aacggtacgc ggttttagaa tatttgattg aacatcattc ccatccaacg gctgatgaga
     2401 tttataaagc acttgaacat cggtttccaa atatgagtgt tgcgactgtt tacaataacc
     2461 ttcgcttatt tacttcgatt ggatttgttc aagaaatgaa gtacggcgat gcttctagcc
     2521 gatttgattt cagttcaaag cgtcattatc atgtaatttg tgaaaactgc ggtaagattg
     2581 ttgattttca ttacccaggg cttgaagata tagaaatggc tgcaagcaga ctgactgatt
     2641 ttgagattaa tgaacatcgg ttggaattgt atggactctg tccggattgt aaagagtcag
     2701 caaaaaaggc gcaaatgtaa aaacagtttc tacaagtgtt atacctaaaa aaagctagct
     2761 gtgtaaaaaa aacgctagct tttttatttt tttgtctttt attcacaatc ttagatataa
     2821 aagtgaatat tattaaaata agaatcttgt agatattggg ttcttatgct tgaaaaggaa
     2881 tgaaactaca aacaaacctt tgtgaattca caagcattaa actgttgtat ccgttttatt
     2941 tctgttatag tagttttgtc gaaggacaaa atgttaatcg gagggaccat aaatgaaagt
     3001 agtagtaatt ggctgtactc atgcaggtac tgcagctgta aaaagtattt taaaaaatca
     3061 tccagaagca gaagtgacag tatatgaaag aaatgacaat atttctttct tatcttgcgg
     3121 tatcgcactt tatgtcggcg gagtagtaaa ggacccagca ggcttgttct actcaaatcc
     3181 tgaagaatta tcttcattag gagcaacagt taaaatggaa cacgatgtaa cagacgtcga
     3241 tacagaaaat aaaacagtaa cggcaaaaga tctgaaaaca ggagaaacag aaacggtttc
     3301 ttatgataaa ttagtgatga caactggttc atggcctatt atcccaccga ttagtggaat
     3361 cgatgcgaaa aatgtactcc tatgcaaaaa ctacaatcaa gcaaatgaaa ttattgcaca
     3421 agcgaaagaa gcaaaacgtg tagtaattgt tggtggtgga tatatcggta ttgaattagt
     3481 agaagctttt gtcgaatctg gaaaacaagt gacattgatt gatggattag atcgcatctt
     3541 gaataaatat ctagataaac cattcacaga tatcttagaa aaagaattga cagaccgcgg
     3601 tgttactctt gcgctaggcg aaaatgttac tgaatttatc acagatgaag aaggaaaagt
     3661 gaaacaagta gctacaccaa cagatacatt tgatacagat atggtcattt tatgtgtggg
     3721 cttccgtcca aatacaaaac tgatcgaagg aaaagtagat acactgccaa acggggcaat
     3781 caaagtgaat gaatacatgc agacaagtaa tccagacatt tttgcagctg gagactctgc
     3841 agtagtcaac tacaatccaa gtggcacgca aaactatatt cctttggcaa caaatgccgt
     3901 tcgtcaaggt ttgcttgtgg gaaataactt gacagatcat aaaatggcgt atcgtggtac
     3961 tcaaggaact tcaggacttt acctatttgg ctggacgatc ggttcgacag gtgtgaccaa
     4021 agaaagcgca actttgaacg atctagaagt ccaagcgact gtatttgaag ataattatcg
     4081 tccagagttc atgccaacta ctgaaaaagt aatgatggag ctagtctatg aaaaaggaac
     4141 gaa
//
LOCUS       DACEXQ010000214         4135 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.214, whole genome shotgun sequence.
ACCESSION   DACEXQ010000214 DACEXQ010000000
VERSION     DACEXQ010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4135
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.214"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..283)
                     /locus_tag="IXH04_002397"
     CDS             complement(<1..283)
                     /locus_tag="IXH04_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1852961.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTI"
     gene            complement(285..1724)
                     /locus_tag="IXH04_002398"
     CDS             complement(285..1724)
                     /locus_tag="IXH04_002398"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAQ1852962.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            1909..3855
                     /locus_tag="IXH04_002399"
     CDS             1909..3855
                     /locus_tag="IXH04_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAQ1852963.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1336 a          698 c          674 g         1427 t
ORIGIN      
        1 taatcgtttt atatccatgt ttaaataact ctatttcgag atactcaatg agttcagagt
       61 aaaagatatt actaatatta ggaaaaataa gaccaataag ctgaggtgac tttccttgaa
      121 gtccacgtgc taaattattt ggtttataac caagagtttt catagcttca tgaacttttt
      181 gttttgtttt ttctgataaa tatcccttat tattaatgac gcgtgaaacg gttgttgggc
      241 taactccagc taattcagcg acatcagtta attttgctac cattttaata ttttaattca
      301 aagtattttc cagtaggttc gcctgatttg atttgaatac cattttggtg attttcaggg
      361 aatacacgac tagtaaacac tttttctcct ttattaataa aaatctcaat gattgagtta
      421 tctacaaaaa tgttagcagt tgtttcttta gtatcaattg agcattcacg aatcgtacca
      481 aagtcagtgg catattgaat gcctgctttg ctgcgatcta aaataatttt tccttctttg
      541 gtatctacaa tcaagctaag tccatttcct ttactatcag agaaaagaag taactccgat
      601 ttctgatttg gaggaaaagt tagttcaagc tcataagtat tatttgtttc tgcttttgct
      661 gtaaattgtt cagaactagc acgtaatgat gtaatagctt ctactggata ttggtaaagt
      721 ttaccatctt ttattgaaag ttcttttaca agactcattg ctccctgata atcatactta
      781 tcagttggat aatcgatatc aggtagtcca atccaactga caatcagagc acgaccatca
      841 ggagtattga atccttgagt cgcatacgct tcaaatccat aatcaagatt ttgaatttct
      901 gaagcgccca caagttttac tttttctatg tcaagcgatt gacagatctt aaaagtattc
      961 ggatagatat tcccataatt tagctcagat ttatccaacc cttgaggaca ataaatgaga
     1021 acaggttttt tgtctacaaa tactagattt ggacactcaa tcatgtattc tgaaccagtg
     1081 ccgccaaaat caaggttgcc aacttcctcc catgtttcca cgttattgtc atccgctttg
     1141 taaagtttga taaagcctga tttttctaaa ttttgtgctc caacaatcgc atacaattgt
     1201 ccttcataat taaacatttg tggatcacga aaatgatcag ttacatcagc aggtttatga
     1261 ataagaacat tctcaccttt cgtaatgttg tagtctttat ccatccatgc tccgatttga
     1321 agtgtatcac gttcccaatt ttcatttcga acgtttcctg tatagagtaa gaatagattc
     1381 ccctctattt catatgcaga accggaataa gcgccgtggc tatctcgttt tgtgtctgga
     1441 agaagttctg ctcctgttac atgaaaatga actaaatcat ctgattcatt gtgtacccat
     1501 tctttcaacc catgagcagc tccaaatggc caattttgat aaaaaagcgt atattttccg
     1561 ttaaaaaaag aaaaaccatt aggatcgttt aacaatcctg ttttaggttc gatatgatag
     1621 ctagcacgcc aaggtgatcg ttttacatta tccttaattt tatccatctc atctttagtc
     1681 caatcagaat aagcacgata tcgtatttct cgaggtaagt tcatatttta ctccttcaat
     1741 ttatcttttt acaactagta tggtaaacgt tttccgttga aaaatcaatc atttcattta
     1801 aaaaaataaa ctatatgaca aacgtttgac atatttttaa gatatgctaa aataaatttg
     1861 catttcagtg aaccgttttc acgtaaaaat aaaaaaggag tgattcgaat gaattacagt
     1921 aaagtagctt ccgacgtaat acaagcggtc ggaaaagata accttattgc agctgcacac
     1981 tgcgctacac gtcttcgtct cgtactaaaa gatgacacga aagtgaatca aaaagcttta
     2041 gacgaaaatc cagatgtgaa aagcacattt aaaattaatg gacaatatca agtgattatc
     2101 ggtgcaggtg atgttaattt cgtttatgac gaacttatca aaaaaactgg actttcggaa
     2161 atttcaacag atgatttaaa acaaattgct agtaaagata aaaaattcaa ccccattatg
     2221 gcattaatta aattattatc agatattttt gttccaatta tacctgccct tgttgcaggt
     2281 ggtttattaa tggcattaca aaacttttta acagctgctg gactttttgg accaaaatca
     2341 attgaagaaa tgtatcctgc gattgaaggt atttcctcaa tgatccaact gatgtccgct
     2401 gcaccattca tgttcttacc aattttagta ggtatttcag ctgctaaacg ttttggagca
     2461 aaccaatttc ttggtgctgc aattggtatg atcatgacaa caccagatct tggtggctca
     2521 actgaatatt ggaatatttt tggttatcat gtttcacaaa caaactatgc ttatcaagtt
     2581 attccagttt tagcagctgt ttggatatta tctatattag aaaaattctt tcataaaaaa
     2641 ttgccatcat ctattgactt tacattcacg ccattattat cagtaatgat tactggattt
     2701 ttgactttca cagttattgg ccctgtaatg ttattgttgt ctaatggaat tacagacatt
     2761 attgtttggc tatataacac tactggattt attggaatgg gtatttttgg aggaacatat
     2821 tcacttatag tcatgactgg tttacatcaa tcatttccag ctattgaaac tcaattactt
     2881 tcagcgtgga caaatggtat cggacatgga gactttatct ttgtagttgc atctatggca
     2941 aacgttgctc aaggtgctgc aacatttgcc atttggttct taactaaaaa ttctaagaca
     3001 aaaagtcttg cttcctctgc gggtgtctca gcacttcttg gaattacaga gcctgccttg
     3061 ttcggagtta acttaaaata ccgctttcca ttcttttgtg ctttaattgg ttcaggaatt
     3121 gctacagcag tagcaggatt acttaaagta gttgctgttt cacttggttc agcaggtttc
     3181 ttgggatttc tttcaattaa tgcaacatct attccatttt acgtattgtg tgaattgatt
     3241 agctttgtag ttgcttttgc aattacttac ttctacggta aaacaaaagc tgctggtatc
     3301 tttaaagcag aagaaattgc tgaacaaaca ccagttaatt cttcaaaagt agcaacaaca
     3361 gatccaatat ctactgatgt tgaatcaaca caaacaaaag tttcatcaga aacaattgtt
     3421 agtcccattt ctggtaaagt gattagtctt tcttcagtaa atgatccagt cttttcatca
     3481 gaagctatgg gaaaagggat tgccattaaa ccaaatggaa acacggtcca ttctccagta
     3541 gatggtacta ttcaactcac gtttgaaact ggacatgctt atggtattaa atcaaataat
     3601 ggtgctgaag ttctaattca tattggtatt gatactgtat caatgggagg aaaaggattt
     3661 acacaaaaag ttactactaa tcaaaaagtt agaaaaggcg atattcttgg agcatttgac
     3721 cgttcaatga ttgagaaaga aggtcttgat gatacaacaa tgattattgt gaccaatact
     3781 tcggattatt cagaagtaaa tatagtagct gaaaatacag tcactgaggg tactgattta
     3841 cttgaaatca aataaatata tttccctgag taaagatgga attttctttc caaaatcaaa
     3901 taatatagat taatcaaata tttgagaata acattttatt taatcttttc atatatcaat
     3961 tgtatgaaaa attattagaa ttttaaaaaa tgataaaaat tcaaaaagaa aaaatagtag
     4021 ttatttactt gtcatgttac taaataaaaa aataatttaa atatgatata tagtcattta
     4081 tttagtgatt tcattctaaa ataaagggag aaatgtctat gctatacggt gcaat
//
LOCUS       DACEXQ010000215         4095 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.215, whole genome shotgun sequence.
ACCESSION   DACEXQ010000215 DACEXQ010000000
VERSION     DACEXQ010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4095
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.215"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(61..1215)
                     /locus_tag="IXH04_002400"
     CDS             complement(61..1215)
                     /locus_tag="IXH04_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAQ1852964.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            complement(1193..1888)
                     /gene="vanR"
                     /locus_tag="IXH04_002401"
     CDS             complement(1193..1888)
                     /gene="vanR"
                     /locus_tag="IXH04_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAQ1852965.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            complement(2102..2677)
                     /locus_tag="IXH04_002402"
     CDS             complement(2102..2677)
                     /locus_tag="IXH04_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016204422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1852966.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            2823..>4095
                     /locus_tag="IXH04_002403"
     CDS             2823..>4095
                     /locus_tag="IXH04_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
                     /protein_id="HAQ1852967.1"
                     /translation="MKIARGRELLTPEQRQAFMQIPEDEWILGTYFTFSKRDLEIVNK
                     RRREENRLGFAVQLAVLRYPGWPYTHIKSIPDSVIQYISKQIGVSPSSLDHYPQRENT
                     LWDHLKEIRSEYDFVTFTLSEYRMTFKYLHQLALENGDAIHLLHECIDFLRKNKIILP
                     AITTLERMVWEARAMAEKKLFNTVSKSLTNEQKKKLEGIITSQHPSESNKTILGWLKE
                     PPGHPSPETFLKIIERLEYIRGMDLETVQISHLHRNRLLQLSRLGSRYEPYAFRDFQE
                     NKRYSILTIYLLQLTQELTDKAFEIHDRQILSLLSKGRKAQEEIQKQNGKKLNEKVIH
                     FTNIGQALIKAREEKLDVFKVLESVIEWNTFVSSVEEAQELARPADYDYLDLLQKRFY
                     SLRKYTPTLLRVLEFHSTKANEPLLQAVEIIR"
BASE COUNT         1212 a          815 c          742 g         1326 t
ORIGIN      
        1 tattctaaga tttctcttta ccaacatcat ttgacaaaga gccaaaatta tatacatctc
       61 ttaggacctc cttttatcaa ccaagtctgg catcgctgga agctctaccc taaacgtcgt
      121 atagttatca ttgctttccg cgtaaatctg ccctccatgc tgaacaataa tttcttttgc
      181 aatcgccaat ccaagtcccg cgccacccgt atcggaagaa cgagcattgt ccagcctata
      241 gaacttttca aatatggcag ctagcttatc ttttgggatg cttccagtgt tcttgaattc
      301 gattgacacc acatccccgg agaggcccgc ggtaatgtca atgatgctgt tatcctcact
      361 gtatgcagcg gcgtttttca aaatgttgtt aaagactctc gcgagtttat cagggtcgcc
      421 ggacacggtc agatcctcgg gggcgtgaat aaccgcctgt tttccatgtg cggaaagctg
      481 aggataaaat tcatcggtca tctgcaccag catatagtat aggtctatgt gcgtttttgt
      541 tagcgttatc gtttgtaggt tataccgtgt aatctcaaaa aactcgtcga ttagctgttc
      601 gagtcgatac gctttgtcca acgtgatatg cacatacttt gccttttgat ctaccggcat
      661 gtctggagcc tcgtcaagca ggctcaaata accgataatg gatgtaaggg gcgttttaat
      721 atcgtgcgcc aagtacataa caacgtcatt ttttctttgt tcggccagct ttgcatcctg
      781 ctctcgcttt tccagagtcc gttttaatgt gttgagcttt tgttccataa catccatttc
      841 cgcagaaagc tcaatttgtt tatcttcgtt ctgaataagt acatcaatgc cggtatttat
      901 ctcgtcaaag tattttgcga attttgaaag catgacgcga catagaataa gaatactaat
      961 gacaatcgcc acataaataa agatatctat attgttccgt atggaatatt gatataattt
     1021 catcgcgtcc aggtgattta agtcatattt gttttccaaa atacttaaga tccaatcccc
     1081 aagtttccct cggatcattg aacgaatata caacacgaat acaattgcta ccacaacaat
     1141 tgcaacgata tacatgtaaa gttttcgttc tagtttggaa tagtcgtttt ttttattttt
     1201 caattttata accaaccccc cataccgttt ttatatattt cggattatca atggtgtcgt
     1261 tcattttttc gcgcaaatgc cggatatgca cggtgatggt gttgttgctc ttgctgaaat
     1321 attcgtcgcc ccatatctca tgaaatagca gctcggagct aaccacattc cccttgtttt
     1381 cacagaggat tcgcagtatt gaaaactcgg tgggagtaag ggataactgc ttctcgttca
     1441 gataacactc atgggtgtta acattaatga caaggccgga gtggacgata acattttcgt
     1501 tctgctcctt tactccactg aattttttgt atcggcgcaa ctgggccttt acccgagcaa
     1561 ttaactccag tgggcgaaag ggcttcgtta tataatcatc cgcgccgatt gttaaccctg
     1621 taattttatc tacctctgta tctttcccgg tcagcatgat aatcggatag gtgtgcttgt
     1681 cccttatttt ttgacagata gtaaggccgc ttgtgccggg aagcatgatg tccaatatgg
     1741 caaggtcaat ctcagacttg tctatacatt ccaatgcttc tttggcggta tagtatttga
     1801 aaaccgtata attctcgttt tttaagtata attcaaccaa atcggcaatt tcatgttcat
     1861 catccacaat aagtatttta tcgctcatag ttatcaccct ttcacataag tttgccctta
     1921 ataatttatc agattatagg gccgaattta taagcattta cataataaat gactaagaat
     1981 ttcttaagat gatgattctt tttttatcaa agggcttttc ggcatcgcta ggaggctgtt
     2041 aatattttat agtttccttt tcttctttaa aaatattgcc cattagcgga atacagaatg
     2101 gctaattatt cacttctgat aatttcctgt ataatgaagc cctagataca ttagtaattt
     2161 cacaaatttg atttacagtc atatttcctt ctttatatag ctttaccgca taattcattc
     2221 ctgcgtgatt tttatgatac ttctttaatc gacctttaaa ctttccttct ttcttagcca
     2281 attcaatccc ttcacgttgt ctcatccgaa taagatctcg ctctaattgg ttaacaccag
     2341 ccattacagt aattaagaat tggctgtatg gattatcttc tgataaatca agccatgtat
     2401 cttttagtga ttttaaactt gcctttttat ctcgtatgtt atcgattaat tcaaatagat
     2461 cttgtgtact acgagtgatt cgagttaagt ctgtaacata aatgatgtca tcttcctgta
     2521 aatcgtctaa cactttttga agttgctcgc gatcctttgt tgctcctgaa actttctctt
     2581 catatataat atccattccg atctcgttca actgctgaaa ttgtcttgaa ggattctggt
     2641 tagtcgaact gacacgaata taaccgattt tccgcaaaat atcacctcat ttttgagaca
     2701 agtcttatga gacgctctta actatgattt tatcagtcta ctacatttgt atcaatagag
     2761 tacactctat tgatatataa ttgaactaat aaattgaaaa tacagaaatg gaatgatact
     2821 gaatgaaaat tgcgagaggt agagaattgc ttacaccgga acagagacag gcttttatgc
     2881 aaatccctga agatgaatgg atactgggga cctacttcac tttttccaaa cgggatttag
     2941 aaatagttaa taagcgaagg agggaagaaa accgtttagg atttgctgtt caattagctg
     3001 ttcttcggta tcccggttgg ccatacactc atatcaaaag catcccagat tcggtcatac
     3061 aatatatatc gaaacagatt ggtgttagtc catcctcgct tgatcattat cctcaaaggg
     3121 aaaatacact ttgggatcat ttgaaagaaa ttcgaagtga atacgacttt gtaactttta
     3181 ccctgagtga atatcgaatg acatttaagt accttcatca attagctttg gaaaatggtg
     3241 atgccattca tctactgcat gaatgcatag attttctaag aaaaaacaaa attatactgc
     3301 ctgctatcac tacacttgaa agaatggtgt gggaagcaag ggcaatggct gaaaagaagc
     3361 tatttaatac ggttagtaaa tctctaacaa atgagcaaaa aaaaaagctt gaagggatta
     3421 ttacctcgca gcatccatcc gaatccaata aaacgatatt gggttggtta aaagagccac
     3481 cgggtcatcc ttcacccgaa acttttctaa aaataataga acgactcgaa tacatacgag
     3541 gaatggattt agaaacagtg caaattagtc atttgcaccg taaccgcctg ttgcagctgt
     3601 ctcgcttagg ctcaagatac gagccgtatg cattccgtga ctttcaagaa aataaacgtt
     3661 attcgatatt aaccatctat ttattacaac ttactcagga gctaacggat aaagcgtttg
     3721 aaattcatga taggcaaata cttagtttgt tatcaaaagg tcgtaaggct caagaggaaa
     3781 tccagaaaca aaacggtaaa aagctaaatg agaaagttat acactttacg aacatcggac
     3841 aagcattaat taaagcaaga gaggaaaaat tagacgtttt taaggtttta gaatcggtta
     3901 ttgaatggaa tacctttgtc tcttcagtag aagaggctca ggaacttgca cgtcctgccg
     3961 actatgatta tttagactta ctgcaaaaac ggttttattc actaagaaaa tatacgccaa
     4021 cgctattaag agtattggaa tttcattcta caaaggcaaa tgagccactt ttacaagctg
     4081 ttgagattat ccgag
//
LOCUS       DACEXQ010000216         4079 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.216, whole genome shotgun sequence.
ACCESSION   DACEXQ010000216 DACEXQ010000000
VERSION     DACEXQ010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4079
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.216"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            133..417
                     /locus_tag="IXH04_002404"
     CDS             133..417
                     /locus_tag="IXH04_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1852968.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            420..1337
                     /locus_tag="IXH04_002405"
     CDS             420..1337
                     /locus_tag="IXH04_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1852969.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            1330..2112
                     /locus_tag="IXH04_002406"
     CDS             1330..2112
                     /locus_tag="IXH04_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1852970.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            2113..2793
                     /locus_tag="IXH04_002407"
     CDS             2113..2793
                     /locus_tag="IXH04_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1852971.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            2786..3682
                     /locus_tag="IXH04_002408"
     CDS             2786..3682
                     /locus_tag="IXH04_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1852972.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            3877..>4079
                     /locus_tag="IXH04_002409"
     CDS             3877..>4079
                     /locus_tag="IXH04_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein"
                     /protein_id="HAQ1852973.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDS"
BASE COUNT         1252 a          704 c          908 g         1215 t
ORIGIN      
        1 gttggcagga gaacaccgtt tattcggaag aagggattgt gaaaagtctt ttgtcataac
       61 ctcttatttg gtagctaatt gtaaatccca aacggcatgt attatttttc ataagtccgt
      121 ttttaaggat taatgttttc ttttgctttt tatatggtct ggcttcagcg agaagttcca
      181 acgcatatac aaggacgtct gatgtcaacg ataacaacga ttaccatggt ggccagtcca
      241 cttggattag cactgtttgg tgcgctcttt gattggcaga caactcaacc acgtctgcga
      301 gacagtctga tttttcttga aattcaattg gaaaatttac actttcttta tacatttctg
      361 gctgctttct ttatacccgt caactatact tcacttatca ataagaaaga aggataagta
      421 tggattttgg aaatgaaacg gtcttgaaag caactggtat aaccaaaaaa tacggtgctg
      481 ccaaagcatt agataaagta tcgatcgaaa tcaaacgtgg aatgatctat ggtttgattg
      541 gggagaatgg agcgggtaag tctaccttta tgagaactat tatgggtttg atctcgattg
      601 atgaaggttc gatcgaatta tttggaacga cggatttgca ggcagcaaga cgacgtatgg
      661 gtcaatcgat tgaaacccct gctctttatc cagaattaac tgcaagagat aacctacgta
      721 tccaagcagc gaacggcggt gtgtcggata gagaaatcga agacctgctg aaaatgatgc
      781 gattggaaaa tacagggaaa aagaaagcaa agaatttctc tttaggtatg cgccagcgtt
      841 tagctattgc aaatgcactg atcacgaatc cagaattttt gattttggat gaaccgacga
      901 atgggatgga tccagcaggg atggcagaaa tgcgagaaat catccaacgt ttggtaaaag
      961 aacggggcat caccgtcttg ctttctagcc acttgctaga tgaattgtca cagatcgcca
     1021 ctcactacgg tattttgcat gaaggacact tgatcaaaga attatcgaaa gaagaattgg
     1081 cacaagaatc tcgtcaattt ataaaaattg acacttcggc gacggaacaa gcagtaacgg
     1141 ttctcgactc tcttggttat agggattatt ttgtccaatc tagccgtgtt atccagcttt
     1201 ttgaaggaat tgaccaagta gcagccatca atcaagcatt ggtagaggca aaagttcctg
     1261 tggatggtat ccacctagtt gggcaaaaac tggaagatta tttcttacaa ttaactggag
     1321 gcaatccaca tgtttaatct attaactgct gaaaaaataa aagtatggaa gagccgtaaa
     1381 atgtgggtct cactaggact gatggtcgta cttccgcttt tttattcttg gaacgaatgg
     1441 tatatgcata caaaatacgg caatgaatta aaccaagcaa cggatacagt catcaacggt
     1501 gcaacaggga ttttaatggt ggaaaaaatg gctgcatgga ttcttctggc tttcgcagca
     1561 tttgcgtgct tttatattgg ggaagaattt caaaacggta cgattcgcaa tacgttatct
     1621 ttaggacgga atcgaatgac ttattatgtg tcgaaacttc tcgttacgct tttgatcaca
     1681 acacttggcg tggttttgat tacggggcta gccatgattg cttacacact tgctttcgga
     1741 tttggtgaag ttgagggaat caaaaactat ggaaattatg ttctaaaagt atttcctgta
     1801 ttgcttctgc tgatattagc gacattatct gttccagtgg ctttgacatt catcactaga
     1861 agtaccagtg tcagtttgct tctttctttt ctttatatca tgggaacggc cttcgttccc
     1921 ggtgttttcg caaaaatcaa aggcttggaa tttctaacgg agtggtttac ggaaacgtgg
     1981 ctcatgtata cggactttgc gcaacaagca acttattcgc aagcaccgaa aatgattctg
     2041 gtcagtctag taacaatcgt ggtatcatca gctctaggaa tgtttatttt ccaaaaatct
     2101 gacattaagt aggtgttcct gctggaaact attttgatta tcgaagacga tgaagctgtt
     2161 cattctttac tggaggaggt tcttgaacag agatacaaac ttcttgatgc ttattcaggt
     2221 acggaaggaa aattattgtt aagcacatat cctgtcgatt tgatccttct agatctcatg
     2281 cttccagggc tttcaggtga agcattgtta gcagaaatcc gacagacatc gaatgttccg
     2341 atcatcgttc tttcagcaaa aagcgatcaa agagataaag tttccttgtt ggcagcagga
     2401 gcggatgact atgtgacgaa accatttgat atcgaagaac tgttgttacg aatcggcatc
     2461 caacttcgtc atcagacttc tgtccaagta aaagatctgt cgcaacgcat ctattacaag
     2521 gaaatcttac tggacaaaga gtcgagggat gtaaatgtga atggtgtggc tgtccatttg
     2581 acaggaagag aattcgatct gctgaagctt tttttagaac atccgaaaaa agtattttcg
     2641 cgagcaaatc tttatgaaac agtttggcaa gaaccatatt ttgatagtga gaaaacagtg
     2701 aatatgcata tttccaatct tcgagggaaa ctagcaaagg cacatgctgt gtatattcat
     2761 acggtgtggg gagtggggtt tagatttgat taagtggctg ttgcttctcg ttgttatctt
     2821 tatgggaagt tttattttct tcctgcaaag ggaaatcaag agattaacta agaaaatcgc
     2881 cactctttct gaacgacaag aatatggtgg tcgtctttca gtcgaatttc gagaaaagaa
     2941 tctaatggac ctagtagatg cactgaatac atgggtggat gaaaatgaac aaaaaggaca
     3001 agcatttcaa gaaatggaag aaaacatcag actatcgatc gtcggccttt ctcatgatct
     3061 cagaactcct ttgactgcta ttaacggtta tgtacaatta ctggaacagg tagaggatac
     3121 ggagaaacgg gagaagtatt taacgatcat caaacaatct gttgcaagat tactcagtat
     3181 gacggatcag ttttatgatc ttgctcgaat agagacgaag caaaaagaaa tggatctcca
     3241 tacgctttct ttttctaaag aggtggaaga agtatttttc tctttttatg aacaattttc
     3301 tgagaaaaat atcgaggtag cgttcgctga acactcgata aatacaactg tattagcaga
     3361 tccgctcatg ttgctgcgag tgctgcaaaa tatcatacag aatttattgc gttatgcaga
     3421 tggacaagca aaggtgacat tttcaaaaga ggattcattc attcgattaa cagtggccaa
     3481 tcctatcaaa ccaggagcgc aaacctctgt tgaaaaagtc ttccagcgtt tttataccga
     3541 aaataccagc cgcacaaata cggaatctag cggaatcggg ctttacctgt caaaaaaatt
     3601 ggtggaagga atgggaggag agatgaccgc tagattagac gggggcatct tctccatctc
     3661 cgtcaagttg cggagggttt aattggctct aatttacctg aagtgaatga caaaaaattg
     3721 tatctcgttt taaagacatc ttcttttttg gaaggtgttt ttattttggt gtgattgata
     3781 cgaattaggt aacgaagaca gttatttttg tacccccttt tcaaatagcg caacagtgct
     3841 atactaaaac taacaaatca aggaacggag agggatatgg gccaaagaga atttatcgtt
     3901 ttaattatta ttgcagttgt tattttatta gttgttttag acattttttc acgtttgaaa
     3961 gtaaaggaaa cagttcgtag taattgggga aaaattcctt atcaaccgag atttgataaa
     4021 gaagaaagtt tgaaggatgc gtggctcacg gagaaaaaat tccgttcatg ggatagtga
//
LOCUS       DACEXQ010000217         4033 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.217, whole genome shotgun sequence.
ACCESSION   DACEXQ010000217 DACEXQ010000000
VERSION     DACEXQ010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4033
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.217"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(175..1461)
                     /locus_tag="IXH04_002410"
     CDS             complement(175..1461)
                     /locus_tag="IXH04_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ1852974.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(1454..2722)
                     /locus_tag="IXH04_002411"
     CDS             complement(1454..2722)
                     /locus_tag="IXH04_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ1852975.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            2974..3435
                     /gene="mscL"
                     /locus_tag="IXH04_002412"
     CDS             2974..3435
                     /gene="mscL"
                     /locus_tag="IXH04_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAQ1852976.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(3494..3940)
                     /locus_tag="IXH04_002413"
     CDS             complement(3494..3940)
                     /locus_tag="IXH04_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1852977.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
BASE COUNT         1138 a          821 c          710 g         1364 t
ORIGIN      
        1 attacttcgt ttgattttat tttaatataa gttgattaaa agagagcatg atttgtttta
       61 ttttctcact aatttttcca acacaactaa gaataccata aaagctgatt ttggaggagg
      121 ttcctactac aagtctaaaa cgaaatttaa gacttttcct cataaaatac tggtttattt
      181 tggtctcatg taaaactcgc tgaaggcctc ttcacttata aatgcttccc cagctgccag
      241 tacgtctttc atttggatcg aatcgatgat ttcaggaaga tcaaataaag ttcgatcgcc
      301 aaataaactt tgcgtaaatt gattggcaat atattcgatc gaatttaatg attggaaata
      361 ttgtccaagc attttcttct tcagcaaatc gagatttgtt tctgacactt ccggatcatc
      421 agcgaatcca agaatgattt ctttgacttt ttctgctgct ttctctggtt cgtccgtatc
      481 tccgctgaag tccgcaaagt gaaattcacg atccaagctg aattcaaaac caaagctgtc
      541 atcgataatt ccttgattgt acatcgctag atagtttcga gaagtattgc ctaaaatcat
      601 ttggaacaac agattgattg ctgttttata gcgtaacagt tctgttcctt cttgcggcaa
      661 tgtgtctaat cctttaatac caagtacaaa tttatctcgt gtaatggctg cttccaatgc
      721 actttgtttg atgatttctt ttgtgttttc agggaaataa cgtacaattt cttgctttgg
      781 agggaaattt tttgcttctt ggttttcccg aataagtttc atcaatttct caggctccat
      841 cttaccaact acaaataaaa ccatattgct tggctgataa aacgtccgat agcacgtata
      901 caagtcttga gctgtgattt tgtcgatact ttcaaccgtg cccgcaatat cgatatgcaa
      961 tgggtgtgta ggatacaagt tatttagtat gccaaaaaac atccgccagt tagggtcgtc
     1021 ttcatacatt tggatttcct gaccgatgat tcctttttcc ttgtttacag tttcttctgt
     1081 gaagtaaggt gcttgtacaa aatcgatcag agtcgtcaga ttcttttcta cctgatccgt
     1141 cgtagaaaac agatagctgg ttttcgtaaa acttgtgaaa gcattggcgg aagctccttg
     1201 tttcccaaat aattgaaaga catcaccatc ttctttttca aacaacttat gttctaaaaa
     1261 atgggcaatt ccatctggaa ctttcacctt ttctttttca ccatagggaa taaattcgtt
     1321 atcgatcgaa ccataattcg tactgaacaa accatatgtc ttgtgatagt catttttagg
     1381 caacaaatac acggtcaaac cgtttggcag tacttcatga taaagtgttt cattgatttg
     1441 ctcatattct gttttattca tttgcatttc ctcctgctaa aaagaagatt gcctgcaatt
     1501 cgatttgctc agctacttgt tgtatctctt tgatcgttac gctctccata cgtttcaacc
     1561 attcttcatc agataatttt gtttccggca gccagctgtc caaaaaagaa gcttcgattg
     1621 ccgcttgcgg agagtctaga gaaagcagat attggtttct cagcattgct tttgtctgag
     1681 acagttccaa ctctgttatt tcaccatttc gcaaactttc taattgctcg tgaatcagat
     1741 gaagtacttg atttcggttc ttttcatcga taccggtttg gacactcatg aatcctcgga
     1801 acgtatccac gctactagag gcataataag ctagactttc tttctctctg acattcatga
     1861 aaagtttaga gtgaggaaaa ccgccaaata aaccgttaaa gaccattagc gcaaaacgtt
     1921 ctggttcatc gtaatacaca tttgtctgat aagctaggtt cagctttgct tgaacgattg
     1981 gctcacgtac ttgtcgttcc ttcacgatat tcacttgagg ttgggtataa aacagatctg
     2041 gataaactgt ttcccgcgac tcaaatggta attgcccaac tgcttcagct gctttgtccg
     2101 gatcaacatc gccgacaaca aaaatgtcga tttgatcatt tgccatcatt tcttgatagt
     2161 aggacgctag cgaactagca gtgatcttgt ccaaatcttc gactacgccc aaccctggga
     2221 tcttttgttc cgggctgttt tcaaaataaa gttcttgcaa ggcataggaa gcaaaagttt
     2281 gtttatcttc cttcaaactt tccaaatata gacgcagatt gttcttttcc aagtcaaacg
     2341 tcaactgatc aaactgatga tttttaatat ttggataaaa taaaacctct tttaaaaaat
     2401 ctaccgcttg agaaaaaagg tttggatcat tgacatattc accattcaca aaactgatcc
     2461 ctgcgtttag ccaatgtaaa tttccttttt ttctaactga taatccaaag cttgcgccat
     2521 aaagctctgc aagttgcgca cttaactttg tctgatccgg ataattcaaa ctatttgttt
     2581 ccaacatgct gctgagaagt gaacgtttcg tgatgacttc ttgttgcaga cgcgcagtaa
     2641 aacggataaa aatccggatc gttttgtatt gttttgttgg taagacatga agattcacgc
     2701 cttctgccaa ttgatacacc atatttatat gctccttttc acagttgatt gcccgcttac
     2761 tcttttaatg ggtcaacctg ccttttttac ttattcctct gcttgaaatt tataagttca
     2821 ggcaattcgc cgttttttct aaatgatgtt acactttata gtatagcata gatgtttttt
     2881 atcaaaagaa acagacggct aaaagcgtat attgtttttt tattttttat ttttcgatat
     2941 actaaagaca aataaaagaa gggagggtca ttcatgatca aagaattcaa agagttcatc
     3001 atgcgtggag atgtcttgga tcttgctgtc ggcgttgtta ttggtagtgc gtttacaagc
     3061 atcgtaaacc aagtggtgca aggcttgatc acaccactaa taggttggtt tgtcgcaatg
     3121 atcacaggta cttctgattt agaaggagca ctttcaattc ttgactggtc gccgacaaaa
     3181 ggcgtcacat ttgcgtttgg taacgttatt agtgctatca ttacatttct gatcactggt
     3241 tttgtcttat tcttagttgt caaagcggca aaccgtgcga agaatttacg ctccaaagaa
     3301 gagcaagtgg aagtggaaga aactcctact gcagaagatt atcttagtga catacgcgat
     3361 cttcttgcca aacaagtggc agaagacaat acacatccag tagagcaaac aaatctttcc
     3421 gatacttcta aataataaag aatctaaaaa aaagaaagcc gattttcagt gtcggctttc
     3481 tttttttaga ttcttaactt gctacttgtt cctttttcat gtcgatatgt ggaatatcat
     3541 cttccaaata cacatctgag attgccttga atccaaacga ctcataaaaa gcttgtaggt
     3601 attcttgtgc gccgatcacg attggttttt ctggaaaatt tttctggatc atgttaatcg
     3661 tttcttgaac aatctttctt cctaaaccat ctttacggtt gtcctgatga acaaggacac
     3721 gtccaaaatg gatactctcc gcttcttcat atactcgcgt gtaggcaagg atcttcccat
     3781 cagtatcttg aaaaaacgta tgccaagctt gtccatcaat gtcatcaact tcttgataag
     3841 gacactgttg ttccacaaca aaaactgcga ttcgttcttt gacgatcgcg taaaactctg
     3901 ctgttgacaa ttcttcaaat ttctttacct gatattccac taagcaaacg ccccctgctt
     3961 ttttattctt agcataccga ttcgcacaca aaaaagcaaa cgaaccttac gaatctcagg
     4021 ctcgtttgct ttt
//
LOCUS       DACEXQ010000218         4023 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.218, whole genome shotgun sequence.
ACCESSION   DACEXQ010000218 DACEXQ010000000
VERSION     DACEXQ010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4023
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.218"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..129)
                     /locus_tag="IXH04_002414"
     CDS             complement(<1..129)
                     /locus_tag="IXH04_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
                     /protein_id="HAQ1852978.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSR"
     gene            complement(298..882)
                     /locus_tag="IXH04_002415"
     CDS             complement(298..882)
                     /locus_tag="IXH04_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1852979.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            complement(1093..1296)
                     /locus_tag="IXH04_002416"
     CDS             complement(1093..1296)
                     /locus_tag="IXH04_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852980.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            complement(1306..1728)
                     /locus_tag="IXH04_002417"
     CDS             complement(1306..1728)
                     /locus_tag="IXH04_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852981.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            2042..2656
                     /locus_tag="IXH04_002418"
     CDS             2042..2656
                     /locus_tag="IXH04_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1852982.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIITSVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            2806..>4023
                     /locus_tag="IXH04_002419"
     CDS             2806..>4023
                     /locus_tag="IXH04_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ1852983.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNA"
BASE COUNT         1332 a          753 c          662 g         1276 t
ORIGIN      
        1 tcgactcata acaacaagtt ctgttgttaa tggatctaaa tttctttcta tacattcgac
       61 acttgcgtaa aagcttttca tatcgattaa aaagtaatca tttactggct cttttgaata
      121 atccatcata aataacactc cattcttcca cattatacaa acatatgttc gatgttgtaa
      181 agaagaaata gctaaatatt tgagaataag ttatcagtag acacttacaa aaaattctca
      241 tactattgtc acttttctag ataaatggca aaaattttca gcattaaatg acaaaaacta
      301 atgagaattt gtctttccat ttttctcctc catttttgcc aaataacgat aaatggtggt
      361 tcttgagacg ttccaccgtt ccgcaatagt cttaataggt gtaccgctat ccactaaagt
      421 cttcagtaat tccaaatctt gttcagacaa tttttcagga cgaccaccaa accgtcctct
      481 tgcacgagca gctgctcgtc ctgctgcgga tcgttccagg atcaaattac gctcaaattc
      541 tgcaaatgcc gcaaacaaat gaaacatgag ctgtcctgta gaacttgatt tgtccatcgt
      601 aatattttct tgaatactat gaaaactcac accacgctca ttcagtcgat tgacgattga
      661 aatcaaatct tccatatttc tacctaaacg atctaagcgc caaacgacaa gtgtatcgcc
      721 agagcgaaca aaatcaatgg ccatttctaa tcctggtcga ttactttttg ccccactcat
      781 atgatcagta aaaatttttt cacaaccaaa tttatttagg ctgtcttctt gtaaatccaa
      841 attttgtaaa ccagtagata cccgggcgta tcctattttc aaaaaaatca ctccttcatt
      901 aattttattg taacataact ttatccatat agtttaactg aacatagata ttgttatggg
      961 tttatgaaca tgaaatgaat tgtttttttg aaaaataaag caaaaaaaga aagtgtccag
     1021 aaatgggtga ttttatgaac actttcaatt agaaaaaaag aatgtcgact agacactctt
     1081 tttttaaaat cattattttt ctttttgata tgttttttgg tctctttttt ttcttttttc
     1141 ttcagaatct tctagattag aaattgattt ttgaattttt tgtgtctcct gtaaagttcg
     1201 ctgaacttgt aaagctgatt cttctttcgg taaattaatt ttacttagaa tatcgctatc
     1261 ttgataaaaa tttggtgtgt tcacaaattc tggcataaaa aatccctact ttctagttaa
     1321 ggttcctgaa taagtcgcaa tccaattgtt tccagctgat ttataacttt gtaaatacaa
     1381 ttttccatag taccaataac catttctttt ttctttccaa ttccgtgatt gaggaggtct
     1441 agaaggccaa ccaactttat caaaaatcat cgttcctgtc actcgtatcg ttttagaagc
     1501 tgctcgagca agtaatggag aaactattaa ataattttca gtaacagtta agtcgttagt
     1561 tgggttacca atggctattc cgttcctatc taaaggagtt aattcttggt tattatctac
     1621 tacagtagtt ggttcatttg tactatcagc aaatgtagga attgtagttc cagttacact
     1681 aattccaata gttaaaatac ctaaagataa aataaactta tttttcattg tctatcacct
     1741 cgaaatcaaa aataagaaaa cgttttctta ataaatctta acagataaat caagttatat
     1801 actagaaaat tcataaaata tatttttaat gtcataatcg tttaaatttt cttgattgac
     1861 atttatttac aaaaaatatt gccatttaaa aagaaaagtg acacatacta tttcctccat
     1921 cattagtttt tgtctatata aggtttaccc taaatagaca ctggtataac attgaaaaat
     1981 atggcttcct cgtgtatgat tagggtgtat tttaaatagt tgcagaaagt aggtaaaagt
     2041 tttgagagtt gcatacatac gtgtctcatc gatcgatcaa aatgaacaac gccagattga
     2101 agaaatgaaa aaattcggtg cagagagaat tttcatcgag aaacaatctg gcgcaacgat
     2161 tactcatcga cctgttttcc aagaagcatt agattacgtc cgagatcaag atatttttat
     2221 tgtggaagct attgatcgac ttggaagaaa ttatgacgag atcatcactt cagtcaatta
     2281 cttgaagaag aaaaatgtga agctgatcat tactagttta ccgatcatgg ctgaagcaat
     2341 tggcaatcct ctcctggata aattcattaa ggatctgatc atccaaattt tggccatgat
     2401 cgctgaacaa gaacggactg aatcgaaacg tcgccaggca caaggaatta agatcgcaaa
     2461 agcaaacggc gtctacaaag ggcggcccaa attatacagt gcagacgcaa aagatcccca
     2521 acgtcgcctt gtttacaaaa gcatcgttga agatcttaaa aatggtgttg cgattgctaa
     2581 gattgctaaa gactacaatg ttacgagaca aaccgtgtat agaataaaaa aagacagcat
     2641 ggtcaatgac aaatagttga tcatgctgct tttattatcg gctctatact ttttgatgtt
     2701 ggtggatgac accagcatct tttttatttt ccaaaacata tagaaaagac accttgaaat
     2761 cctttagaat taatttggcg aaccaaccca aaaggaggaa tcaagatgtc ttacactcac
     2821 cttatcaaag aaacattaga tattctagac ttatcagtta cttttaacga aaattgtctc
     2881 acaaaagaaa aatacaaagg gcaaatctgt catatttatc gtggaaactt gatttataca
     2941 gcccaagaat gcatccactg taaacaccag attgcttcag atattgtgcg ttggggtaca
     3001 acaaccgtcc ggttgttaat gaacgatgtt tctgaatacc gtacctacct cgaattgaaa
     3061 aagcaacgct tcaaatgcaa agcttgtcag cggacctttg tcgcagatac ttctgttgcg
     3121 aaaaaacatt gtttcatcag tgagaaagtc cgctggtctg tcgtgactcg tctgaaaaaa
     3181 aatacctcaa tgacagagat cgccgctcag aaaaatctat cggtctcttc tgtttaccgt
     3241 attatgaagc gattttatcg tccgttgaat ccttttcgga caccgttacc taaggtactt
     3301 tgttttgatg aattcaaatc agttcggggt gtttcaggtg ctatgagttt catcatgatg
     3361 gatggacaaa cacagcgctt attggacatt gtcgagaacc gacaactgcc ttttttaaaa
     3421 cgttatttta gtcatttttc acgagaaatt cgtgaagcgg tcgaatggat cgtgattgat
     3481 atgtacgctc cttatgtatc gctcgttaaa aagctgtttc caaaagcaca gctgattatt
     3541 gaccggtttc acatcgtcca acatattggt cgtacctttc gcaatcatcg aatcaaagaa
     3601 acgaaccaac tattaaaaag caaagaacaa aaacactatc aattaggtaa gcaattaaaa
     3661 agatactgga aacttcttca aaaagatgaa cgcaagcttg attatactcg acgcctttgg
     3721 cgacctggct tcaaagctca tttaacagag accgatattg tggatcgctt actgaaaggg
     3781 agccctgctt tgcgtgtagg ctatcagtta tatcaagact ttctttacgc tgtaaaggaa
     3841 cgagactacg tatcgtttga agaactactt accaataata tcatgcttcc cgaaggctat
     3901 cagacgacct tgagaacgtt ccagaaattt ctgccacaaa tcaaaaatgc tttacaacaa
     3961 agttattcaa atggcccact tgaatgtctg aacaaccata tcaaagtgtt aaaacgaaat
     4021 gca
//
LOCUS       DACEXQ010000219         4015 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.219, whole genome shotgun sequence.
ACCESSION   DACEXQ010000219 DACEXQ010000000
VERSION     DACEXQ010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4015
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.219"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..502)
                     /locus_tag="IXH04_002420"
     CDS             complement(<1..502)
                     /locus_tag="IXH04_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1852984.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVH"
     gene            complement(596..1546)
                     /gene="manA"
                     /locus_tag="IXH04_002421"
     CDS             complement(596..1546)
                     /gene="manA"
                     /locus_tag="IXH04_002421"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAQ1852985.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            complement(1585..2610)
                     /locus_tag="IXH04_002422"
     CDS             complement(1585..2610)
                     /locus_tag="IXH04_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ1852986.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(2819..3688)
                     /locus_tag="IXH04_002423"
     CDS             complement(2819..3688)
                     /locus_tag="IXH04_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAQ1852987.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
BASE COUNT         1119 a          859 c          678 g         1359 t
ORIGIN      
        1 agtgaacact atttaaaact ggttcaataa aaacgattcc aattatacaa ataacgctac
       61 cagtcagtcc tactagtagg ttgctgacaa gcatttcata tccagccttg aatttcacat
      121 ggaatctaga aacaaattgt tcatatacga tgccggcaag cggcccgata atcatcgctc
      181 ctacgatttg tggagcattg gaacgagcga tcaaccctaa agaagcaatt gcaccggcga
      241 ctgccccttt ttgtccccct accgatttcc cgcctgaata actgatcaag atgggtaaaa
      301 gcagctggat catccaatgg catacctctt ctaatgtggt gtgcaaatcg ccagacacac
      361 cttttgttgc taatgacaag attccccaag cgataaacag acttagattt ggcatgatca
      421 tttcatttaa gtgatgtcca gcttttctca gttgtttcat agacacgcga ctgctgttgt
      481 ttgctttcca tttgtattcc atctttttca ccagacttct atttattgat ggacggattt
      541 tatgtagaaa aagcagcaaa cgaaaaaccg tctgctgctt tcagctatct gggaactatt
      601 tgcctggttc agacgcaatg attgctagtt caccttggac cgcccactca gaaacgccat
      661 ttggaataat acagcttgtt cctttttcta atggatatgt ttttccgtca acgacaaggt
      721 ctcctgctcc ttcgattacc gtcatcaatg tataaggggc ttgttttttg aaagatgtga
      781 ttcctttaat atcccactca tatacattga aaaactcagt ttctaaataa gtgacgatcg
      841 atgaattccc ttttcggact tctttgattt gtaattctgg tgttttcgca gggacagttg
      901 ttacatcgat cgattgttgg atatgcagct ctcttgtttg tccttgcgca tcttttctgt
      961 cgtagtcata cacgcggtaa gtcgtgtcag aactttgctg tgtttctaaa atcatgattc
     1021 ctttaccaat agcatggatc gttccgcttg gtacataaaa gaaatcacct tttttaactg
     1081 gtactttctt caaaaggtca tcccagcgcc catctttgat catctcagct agttcttctc
     1141 ttgtctttgc atgatgacca tagatgattt ctgcaccagg ttcagcatcg atgatatacc
     1201 agcattcagt ttttcctaat tcaccttcat gtttcatccc ataggcatca tctggatgta
     1261 cttgtacaga tagatcatct tctgcgtcta agatcttgat caataatgga aacactggtt
     1321 ctgaaggatg gccaaacaat tccttgtgtt ctgaccatag ttcatctaat cttttccctt
     1381 tgaattcgcc attttctact acactgacac catgaggatg cgcactgatc gcccaatctt
     1441 ctccgatttt atcgttaggg atatcaaaac caaagactga acgtaaacga cttcctcccc
     1501 aaattttttc ttgaaaaact ggttttaaaa acataggttc ctgcataatt caaactcctc
     1561 tcatttttca tagctattat agcattattt cggtaatacc gctttctctt tcaaaaagtt
     1621 ttcaacaatt tcatctcgac atatgagata ctccgcacga tgatcgacag gatgtacgcg
     1681 atttgacaaa aatatcaacg cttcttgtgt ttttatatca atcaagaaaa acgtacctgt
     1741 ataaccagta tgaaaaagaa tcgcatgacc gtcttctaca tcctttttta agtcccatcc
     1801 taaagaacga tagccatttt ttttcggtgt ttgatcttgc agtaaagaat caatcgtctc
     1861 ttcttctaaa aaacgaccat tacggatttt tcccttgttc aaatacattt ggacaaaagt
     1921 aatgctgtct ttcaaatcaa taaagagacc cgcgttacct gcatgttcag ccaaaactct
     1981 tgctttcgga tcatgagttt gtccacgcaa aacctcaccc gaaggcagtt gttctgtggg
     2041 aacgattttt ttggaaggaa aaggaagaaa taagctgttc atcattccta atggttccaa
     2101 tacttgttct tgaaagattt ccactgcagt ttcttggtaa atttcctcta gcatcaaccc
     2161 aagaagaatc gtaccggcat ctgtatattt tacttctttt cctagttgct cacctggttc
     2221 tagcttcaaa taagcggcga tcaattcttc ctgagatagc ttatctctgt ttggtatcca
     2281 tgttttgata tcagaagtat gtgtcagtaa atgacgcaaa gtgattgtct gattttcaaa
     2341 ttcaggaaga tatttatgaa gcggttgatc gagttctatc tttccatctt ctaatagacg
     2401 taaaactaat gaagtggttg caacgacttt cgtcagtgag gctgcatcaa acaacagatc
     2461 ttctgtcatc agctgctttt caggaacgac ttgggcaaag ccggctgtaa atgtttctac
     2521 ttgattttct tttatgaagg cgactgctac acctggataa aatccttctt ccatcttttt
     2581 caaaataagc tgtttcgtgt gtggatacat ctttttctcc tgcttttcta tcagattctt
     2641 acagtccaag cttaaagctt aatctatgag gaatcaacct tttttacaca aaaaaactgc
     2701 gcgaaaaacc gcgcagtttc ccccggtata ctattttctc tttctatctc tagaataagc
     2761 gatagtatga ccgttcataa aatgacgaga catgcgacaa tcgttaagaa agaaaaagtt
     2821 aagcgtcgca tcgaatgtca gttttattct tttcttttgg ctcactgacg aaccaccatt
     2881 cgattcctat agcatcatag attgtgagta ctgtgtgctt tttgtctaag aagaaatcaa
     2941 ctgatagttg ctgcaagtga gcaatcagtc cttccatgct ttcagtatcg atcatgaatt
     3001 tcagaaaatc aagtccaatc acttcatctg taggcaaatc aattgcgcct ccgctagctt
     3061 ctgtcaaacc aacatggaaa tctccttcat tcaatacatg gatgccctct tcttcattat
     3121 gtaccttcaa accaagtaca tgattaagaa attggcgttc ttttgttaag ttagtaacat
     3181 ttaaatggat tttttcaaaa taagaacctg ttgatagttt actgaatttt tcttcagatt
     3241 ttcttaataa tgcttcttga tccattgttt ctggaatttc tctttggtcg ttcttattct
     3301 cttcatggta aatttccaat tcattctctt ctgggtctac gagcaaaatc cccatacgtc
     3361 catgatcttc taaagcttct ttgattggat aattcatttt acggatacga caaaggatat
     3421 ctgccatttc ttctgtgcta gggataatca atgaaaaacg gttcagcttt ttgatcaccc
     3481 ccatgtgatc attcgctcta ggactttctt ccagcagcaa catttcactt ccagcttctt
     3541 ctgttcctaa aatggctaat tcgttttctt ctcttttcaa atcaaagcca acaacgttct
     3601 tgtaaaaatc aatcatttta tctcggtctt tcacccgaat ggctacactt tttaaatagg
     3661 tggcagcccc caattgaaaa tctttcatat ttcttgctcc cctctacgtc aagctttatt
     3721 ttaacgatgg attcttcttc tttcaagcaa taactttgat tttgttaaaa aaaatgtgag
     3781 aaaattcgca ttttgcttgt tttttgtaaa ttaatgagta aaaactctta taaaaagagg
     3841 ttgtgaaaaa gctgttctgc atcaagtaat aagaacagcc aaacaaaaat agctcctcct
     3901 atttttcgtt tggttgggct tattaccgca gatgcaagct tttgaacacc gtttaaggaa
     3961 gaggttgtga aaaagtcgct tagtttcgag tagtaagaaa aattttgtga aaatg
//
LOCUS       DACEXQ010000220         3974 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.220, whole genome shotgun sequence.
ACCESSION   DACEXQ010000220 DACEXQ010000000
VERSION     DACEXQ010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.220"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..1101)
                     /locus_tag="IXH04_002424"
     CDS             complement(<1..1101)
                     /locus_tag="IXH04_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAQ1852988.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGF"
     gene            complement(1095..2993)
                     /locus_tag="IXH04_002425"
     CDS             complement(1095..2993)
                     /locus_tag="IXH04_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAQ1852989.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(2987..>3974)
                     /locus_tag="IXH04_002426"
     CDS             complement(2987..>3974)
                     /locus_tag="IXH04_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ1852990.1"
                     /translation="KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESAL
                     YHFSDVHHDVGFLFLPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDH
                     KPGWMIIDSMMNLPLLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDA
                     ESGKYIRLLGGQGYNENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKI
                     EKTGYIPLIDFAVKDSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEK
                     HYANWNMDEDGIIGGGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
BASE COUNT         1155 a          858 c          593 g         1368 t
ORIGIN      
        1 aaagccatct tcttcttccg ttgtagcata tctttctttc aattgtcgca taattttcca
       61 aacagcagac tggtataagt tcttttctgt tttgtctaat agacctgatt ctattaattc
      121 taataatcca caaaccatga tcaccatact agatgtatct ttttcttctc tgctagaatc
      181 atcaaagatc aaatcccaat aacaaatggt atcttctggc aaccgattca taaaataatt
      241 ggctaatatt ttgcttaaat caaaatattt ttgttctttc aaataacgat aacttaatgc
      301 aaaaccgtaa atcccccatg cttggcctct agcccaacag gaattatcac tataaccttg
      361 atgcgtactt ccatatcttg gttttccatc tttgacatcc atataaaatg tatgataagt
      421 tgaaccatct tctcttacta aataatctgc tgcattttga atatgagcct gagcagcttt
      481 tttatatttt tgatttccag tttcttcact cgcccaatac aataatggaa gattcatgca
      541 acaatccatg atcattctac cttgttgtga aggatcacta ggatctcccc aagcttgtat
      601 aactcctgcc ttctcccaaa aacgctgaag taatgtttct gcagcatcaa ttgaagcttc
      661 cttcattttt cgatttcccg ttattttata atctgctacg gttgataaag aatacaaaaa
      721 accaagatcg tgatgattag tgataatttg acagtcaatc ctgtggataa aatctatatc
      781 tgctttttca gctgtttgtt gaaataaagg atcacccgtc ttctcaaata acaaccaatt
      841 aattcctgtc caaaatcctt ctgtccaacc aatattgtct cttaatggat aacgtccttt
      901 tataggacac tcgcttggga attgatactg actgttactg atacgtttga ttaacctctc
      961 cgtgtgtctg cgacaacgct cgatctcatt attccattct ttttccatta attctttata
     1021 acattcattt atccattttt ctggatgtat tatctcttca aacatcactt tatcaagcat
     1081 ttccccacca gcctttagca taattttagt ctttttttat tgtttttatt ttggatagtt
     1141 agcccggttg tcccatcaaa atcaattttg ggacgattgt tccatacttc taaactataa
     1201 tcccgatctt gatgagcaac cactcctgtt ccgagccaat gggttccttt tccaaatgta
     1261 ccctctaatc caggaataaa agtgttttcc catgtcatca agtttgtatt gggcaaaccc
     1321 ataatagctg tggattttcg tttaatagta tcgtcactta aacagataat acctgaaaag
     1381 ccagcttcac tcaccctata gttttccttg tcttcttcta tgattattcc ttctttctca
     1441 ggagaacatt caatagcaaa ccctgtctca tatagaacat attctgtatc taattctact
     1501 ttgtgtacgc gaatctgcca gccttcaaaa ggaataagcc aagttctgat ttttgcctgt
     1561 aaagtaggtt cccatgtaga ggcaagtacc tctttcttac atacaatgtc tcgtacatta
     1621 tttcgaacga tgaactgtcc ataggttttg atgctctcat tcttaaaact ggtcgtgata
     1681 tatgtgtccg ctcgctgaat ccctagcatt gaatccattg ccatttctgc tttcaattga
     1741 ttcgctcgcg gtatcgaaaa accaaattga ctagaataag caaattttaa atatttttca
     1801 ttggtcaatg tatggaaatt tggccctaat tgtcccgcat tcaaaaaaaa tgtatgtcca
     1861 ttatcatgtt gtatgagcaa attggcttct gaaagaagtt tagttccttc acgaataggc
     1921 ataggcattt ctttagccgt ccaatactca tggtcttcag gtagcgccaa caataaaaaa
     1981 atcttattca gccataatgg agataacgta ctattatagg gttcagtcaa tgtcaattga
     2041 gggtaggtat aaccaatatt caggatacct ttttcatcaa atatttcttt ttgcatccac
     2101 catcttatat tccgattgac taaccccttg atttcaccta atttaaaagg caaaagatcg
     2161 gcccacaaca tagctgacca aaagcttgaa actgcaaaac gataaatcat gcttcgacca
     2221 aaaggcacat ttgcaccatc ttccgcaaaa aaatgaatat agtctctaga aaaatcaata
     2281 gctcggttct tatatatctg gctaatgggc ttttctggtt ctagtacgct ataaatcaaa
     2341 ccataataat gaaatgcaaa aggattataa taatcttggc gacctcttga actgtctcga
     2401 taccagccat tttgaagata acaattttct atattatcaa atgcttcttt cagtgctgat
     2461 tctttaactg gataaccaaa tttttggata attgttccga caattatctt aaaaaattgc
     2521 caattaccat ctgcacattc atattcaaaa atttgcgaga accaatgaat aatctggctt
     2581 ttttctcctg tatctaattt tttccataat aattgttcat gatgatataa cgctaataac
     2641 attgcgggca tttctaccat acgctggtct ctttcatgta tttctcccca ataatcggga
     2701 tgggaaggat ctgttccagc ttttattcct gatagaatta atttcaacca tcgttcatct
     2761 aattggtcaa ttgcgggccc cgctccccat aaaacccgag agaatccttc cattcctgca
     2821 attttatttc catatcctac cccttgatgt atataatcaa tttgtgcgcc acttgctcga
     2881 taaaacggct ggagtggttg atttatcttt ttaaataagt caaagtaatc ctgtctggtc
     2941 cttaatagtg gtactggatg tttcattgta ttttctcctc tttttactac caaatagcta
     3001 attccgactg ttctaatcga agtaagcctt caataaaaaa ataatcagaa tagatcagtg
     3061 gcacattata agtcagttct ctatggtatg cttctgctcc gcctccaatg atcccatctt
     3121 catccatatt ccaatttgca tagtgctttt ctgtcttttg gagtatattg atagcagctt
     3181 catgatacat ttttgatcgt acagaggaaa catatcctgc taattccaga agaccgcatg
     3241 cagcgcatac tccagcactg gtatcaatat tcgaatcttt tacagcaaaa tcaatgagag
     3301 gaatataacc agttttttcg attttctgaa taaaatttac tgctatcttt tctgctatag
     3361 ctagatagta cggatttttt gtgtaacgat aacttaacgc gaatccatat aaagcccatg
     3421 catttcctct gctccaacaa gaattctcat tgtatccttg cccgccaagt aaccgaatat
     3481 atttccctga ttctgcatca aattctgcga tatgcccggt tgatccatct ttcctcacta
     3541 gatacttagc tgttgtatct gcatgctgcc gagcaatttt agagaacctc ggatcaccag
     3601 tttccctcga agcccaatac agtaatggta aattcatcat actatcaata atcatccaac
     3661 caggtttatg gtcattccat gctactaaat agttccctga aatattatag cgtccagcaa
     3721 gtaccgtagc tgcatgtaac cctagttgtc gactatgttt gttttgagac tgttgataat
     3781 gtgcaatagc cgatggtaag aataaaaaac ctacatcatg atgaacgtct gaaaaatggt
     3841 acagggcaga ttctaataat tgttcttgtt gtattgctgc ctctttgaac ttttctcgtt
     3901 ttgtcttgtg aaagagctgc cataaaatac cactccaaaa accattggtc caccagtcga
     3961 ttcctctgtc cttt
//
LOCUS       DACEXQ010000221         3943 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.221, whole genome shotgun sequence.
ACCESSION   DACEXQ010000221 DACEXQ010000000
VERSION     DACEXQ010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.221"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..326)
                     /locus_tag="IXH04_002427"
     CDS             complement(<1..326)
                     /locus_tag="IXH04_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAQ1852991.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMG"
     gene            complement(323..1636)
                     /locus_tag="IXH04_002428"
     CDS             complement(323..1636)
                     /locus_tag="IXH04_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ1852992.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            complement(1717..2037)
                     /locus_tag="IXH04_002429"
     CDS             complement(1717..2037)
                     /locus_tag="IXH04_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1852993.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            complement(2145..2432)
                     /locus_tag="IXH04_002430"
     CDS             complement(2145..2432)
                     /locus_tag="IXH04_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1852994.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            2559..3203
                     /locus_tag="IXH04_002431"
     CDS             2559..3203
                     /locus_tag="IXH04_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAQ1852995.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            complement(3263..>3943)
                     /locus_tag="IXH04_002432"
     CDS             complement(3263..>3943)
                     /locus_tag="IXH04_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1852996.1"
                     /translation="QIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSRMTLRQAIQN
                     LADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTISYFLTSPSSS
                     EMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITESLYRTLEEK
                     GGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFEYVRTQYVGN
                     RFEFYLEK"
BASE COUNT         1171 a          838 c          690 g         1244 t
ORIGIN      
        1 cccataaact tgtattgatt gagccaggcc ttcatatttt catcgtacac aaaaaaggca
       61 cggtgctgaa aatcataggt cggatatttc ttcttccatt cttgacagtc actgcagtaa
      121 acgggaatag actttacttt ctttttcgta ctctctagat tgaaatggat tggtttttga
      181 cagccagaac agcctcttgt ttccaataaa tgaaattgtg cgcgacaaac tgaacaagtc
      241 tgaggtaatc tgtatttcga aacaccaata atctcgctga atgttaaatt ttggacgata
      301 tctttactgc agcaactgca cctcacagca tcttcttccc ttcttcattc atttctttga
      361 tttcctgaca agcttttcga atagctgcag ttttttcttc atagaaaaag tatacttgac
      421 tgttcgtata aggcccttta cgatcggctc ttcctgcgat ttgtaccaaa gaagatttag
      481 taaaaaccgg atgatccgct cctaaaacga taacggatat tctttcaaat gtgactccac
      541 gttcaaggat catcgtggtt aacagtatgt catattttgc ttctcgcatt ttctggactt
      601 tttccgcccg ctgattgtct cttgaagaaa catctgtgat ctcatatctc ggaagagcct
      661 cttgaagtgt ggctttcaaa cgatgcatca tcgtgatact tggacaaaat aaaaggatat
      721 ggttttcttt aattaattga tgaatgatcc gcaccaaaga ttttatcttc ttcttgttct
      781 tgcatttttg tcgccaatta ttcatgaaaa ccaagacggg ttcaggaagt aaacgccgat
      841 gaaagcgcaa agcaagtttg tggatgtcaa acgtctgctt gatttccttt agtaactttt
      901 tatcaggtgt ggcacttaag tagatgaatc gtccgtctgg tttaatcgct gtttgcacag
      961 catgagctaa tcctgcatcc ccgttataag gaaacgcatc gatctcatcg atcactatta
     1021 aatcaaagga ccgataaaaa cggtatagct gatgaatcgt acagataaca aaagaagtaa
     1081 aggtataagc cgttgctgta ttcccatgga gcaacgtgat tttttcttct ggaaaaacat
     1141 aacgatagcg taaaaacaat tcattacaaa catcgactcg cggtgtacaa atcgctactc
     1201 ttcctccagc ttttaaagtt tccactaaaa taggataaag catctctgtt ttccctgcac
     1261 ctgttacagc ccaaataaga tgatgtcttt tttctgaatg gcaaagtatt ctctttgcga
     1321 tttccgtttg tttttcagta agctgtcctg tccaaaaaaa gacgacttct cgaggttttg
     1381 cttcaggctg atcaaacaaa tataaatctt gttgagtgtc gcatcgccct aaatatagac
     1441 aatgtgtaca aaaataaccg taatctgcaa gctgtccatc aatgacggac gtcctcattc
     1501 cacagcgatg acaaagaatc cagttgccca aacgttccac tgctgcgatc ttctcataat
     1561 ttggtaaatt tacctcctca cagtctattg ctttaatatc tctcgtcaga agccttctac
     1621 cccaaaaatg attcatatta ttcgccctaa aaaaatatac gaaaaaacaa gcgatctatt
     1681 ttttggatcg cttgttcgat agttatactg ttctatttat tcacctatga gcgtgattgc
     1741 ttgatctaac actttttcgc cattcatcat accataatca gccatattga tgacgtctaa
     1801 aggaatgcct tttggttcca gacgtttttc aaaatcagct ttcataaagc gaacttgagg
     1861 tcctaataac agtacatcta catctttatt tgcaagattg ttatctgcgt cagaagcgga
     1921 cacagcgaaa atatccgagt cgattccttt tgcttcagca gctttttgca tttttgttac
     1981 tagtaaactt gtgctcatac cagccgaaca tacaagcatg attgtttttt tagccatact
     2041 tttctctcct ttttaaaaca aactacgcaa acgttttctt cgcgtctgtt actctattat
     2101 ataaaaagaa agaaatttgt caacgttttc taaacgcggt ttatttaatc aaatagccaa
     2161 ctagatttca ctaatttatt gaacacgaca tattctttgt ccaaattgat gacaactact
     2221 tcatagcctg catttatcca gccataggca cctttttcag ggtgtttata atcatttgcc
     2281 caccaatctg tactttccct tgctgttttg ggaagtccgt tactgggaaa cagcagttcg
     2341 tcaaattggg taaaggtcaa cgttacttga gagccgccat tgttcttcaa ataatgcgta
     2401 attgcatcgt actttttcat tcgttcctgc atccccagat ccacctacct ttgtctttat
     2461 tatcgcatac tacagcgatt gcgcaaccgt tacagtgttg aaacaaccta gaactttatt
     2521 tatactgaat aaagaaaatg aaaaaaagag gtgacaagat gatcgagcgc tatcgtacaa
     2581 taaaagaaaa tggacaaagt gagattgaga tcaaaaaatc acgctttatc tgttctctta
     2641 gacgaataga atcagaggac caagcaaaaa gtttcattca agaaataaaa aaagaacatt
     2701 ggaaagcgaa ccataactgc agcgccttca tcattggaga aaaaaatgaa atccagcgaa
     2761 gcagcgatga tggagaacca agtggaacgg ctggcgtacc gatgttggaa gtcctcaaaa
     2821 aaaatcaact gatcaatgta gctgcagtag tcactcgata ttttggagga acgaaactcg
     2881 gcacaggcgg attaatacga gcatacactc atgctgtctc tcaagcattg tctgacattg
     2941 gaatcgtaga aggtaccttg caacaggaga tctctgtgat gatcacgtat ccgcaactag
     3001 gaaaattaca gaattatctt gaactccaac atttttttat aaaagaaatc caatatactg
     3061 atcaagttag cgtcatctgt gctgtagatg aaaaaagtgc tgaccacttt aaagaatcca
     3121 tcattgagct gctgaacgga caagtccaat ttgaagaggg tcctgtttct tatcacgagc
     3181 aactggtaac tgatcatata taaaaacagg tgattctgat tctcttttat ttctaagtaa
     3241 ggaatcaggg atcacctgtt ttttattttt ccaaataaaa ctcaaaacga tttcctacgt
     3301 attgcgtacg cacatactca aacggcgtac cgtcttcaaa ataagaaatc tggcgcaaac
     3361 gaagaactgc atctcctcgt tttacctcta aatagtcagc aattttctca gaagccaaca
     3421 cagcagtaat cgtttgattc gctggaccga tctttttccc gcctttttct tctagcgtgc
     3481 gatacaacga ttcagtaatc tctgctttgc tgtagccatc gatcagcttt tgtggcacac
     3541 tggcaacttc aaaacaaata gggacatcat ctgcgaaacg aatacgttcc attcgcaaga
     3601 tcgtgtcatc tttactcaag tttagttttt ccatttcact tgagcttggc gaagtcaaga
     3661 aataagagat tgtccgacta gacggtactc gattttgcga ttccataatt tctgtaaaac
     3721 ttgttgcacc agacattttt tcttgtactt tttctcgcgc aacatatgta cccgaaccga
     3781 tctttctttc taagattcct tcatctgcca agttttgaat tgcttgtctc aacgtcatac
     3841 ggctgacatt gaatttcact gctaattctc tttctgaagg aagacggtct ccgactttcc
     3901 atataccttg ttcgatttca ttttttaatt ggtcatgaat ctg
//
LOCUS       DACEXQ010000222         3918 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.222, whole genome shotgun sequence.
ACCESSION   DACEXQ010000222 DACEXQ010000000
VERSION     DACEXQ010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.222"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(34..639)
                     /locus_tag="IXH04_002433"
     CDS             complement(34..639)
                     /locus_tag="IXH04_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAQ1852997.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVASYGMICSARELHLPNAPEKKGILVLPEDAVVGEVFPK"
     gene            complement(835..1299)
                     /locus_tag="IXH04_002434"
     CDS             complement(835..1299)
                     /locus_tag="IXH04_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ1852998.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKALEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKQMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYAIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(1321..1641)
                     /locus_tag="IXH04_002435"
     CDS             complement(1321..1641)
                     /locus_tag="IXH04_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ1852999.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VIKG"
     gene            complement(1784..2863)
                     /gene="pepA"
                     /locus_tag="IXH04_002436"
     CDS             complement(1784..2863)
                     /gene="pepA"
                     /locus_tag="IXH04_002436"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAQ1853000.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            3063..3413
                     /locus_tag="IXH04_002437"
     CDS             3063..3413
                     /locus_tag="IXH04_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ1853001.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKTSFLKEGAIEGAWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLDISRKKGAID"
     gene            3598..>3918
                     /locus_tag="IXH04_002438"
     CDS             3598..>3918
                     /locus_tag="IXH04_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1853002.1"
                     /translation="MESREKIAVLIPCYNEEATISTVIEDFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAAHGLLEK
                     LRSGE"
BASE COUNT         1103 a          801 c          724 g         1290 t
ORIGIN      
        1 aagaggttgt gacaaagact tgtcacaacc tctttatttt ggaaatactt ctccgactac
       61 ggcatcctca ggcaatacta agattccttt tttttctgga gcatttggca aatgcaattc
      121 acgagcagaa caaatcattc catagctggc tacccctcgc aaagttcctg gccagatcat
      181 cattccatct ggcatcatgg ctcctggttt tgcaacgaca actttttgtc cagcttcgat
      241 atttggtgca ccgcaaacaa tttgcaatac tgtcccattg tccacttctg tctcagtaat
      301 cgataaatgg tcagagtcag gatgcgggac gcatgttttt acatacccga caacgatctt
      361 cggttcatga tcagcttcaa gctgttcttc gaatcctgct tctttaatca atcgattcaa
      421 tttcgctaat tcttcatctg ttaagcttac ttggccgttc ccttcaattg ttagatgatc
      481 ggagatatgg aaaaagttcc aagcaacaac ttgaccttgt tcatctttga tccgagcaac
      541 attccattta cgttcgacag tgttttctgt tcctttatcg tcggctatga taaccatcaa
      601 agtgtctccg acgtgtgctt tgttataagc aaaaatcatg ttctttcctc cattatcctt
      661 tgctgagatt tatcttaaca aaaaattgca ttcactgcat gagatttttt tcatgcttta
      721 tttccgaaga agcttcttct ggaacaccat ttattcgtaa caagggactg tgacaagact
      781 tttgtcacag tccctttact gtttgattaa aaaggttata caagttccac ttgcttaatt
      841 actcggctgt tcttcttcgt cagaaacgat caagacatca caaggtgaat gacgtatgac
      901 gtagctggct acacttcctg tcataaagcg ttcgactgca tttagtccag actgcccaac
      961 catgattaga tcgattgcat atttttttgg caatttgact gccatggatt cacgaacaga
     1021 gccgtatgca ataattcctt ctatatttgt gtaatcaaca gattttccat attctttaca
     1081 ttcttcgatc atttgcttgg ctgtttcagt ttgctgatcg actagatttt gatcaatagg
     1141 agcatagccc ataaaattat aaaattgctg atcgattacg tttgctacat atactttccc
     1201 attattacgg cgagccactt ctaacgcttt tttgaaagca gcaagtgctt gttcactacc
     1261 gtcaatacct actaaaatgt tcttatattc ttgattcatc ttgacccctc ctaacagatg
     1321 tcagcctttt attacacttt ctaggaacga ttcgatttcc tgttttgttt tacgatcttt
     1381 attcactaga cgacctaatt cttttccgtc ttggatcacg acaaaacttg ggataccaaa
     1441 aatgttccat tcagcggcta catctatgta cttgtcacgg tctacttcaa taaattgcca
     1501 atcagaaaag tcattttcga tttcaggcat ttgcggtttg ataaaacggc agtctccgca
     1561 ccagccggca gtaaagaaaa atacactttt tccagtttct acgtagcctg ctaattcttc
     1621 taaactattt gggataatca ttctttttcc tctactttct gtttatgtat ttttcaatta
     1681 aattatacac ctttctttcg aactaacaaa cattgatcct ctttttataa aaaaaatctg
     1741 acaaaaggtt gcttttgcca gattttatag tttcactatc gcgttatttc ccgtatacga
     1801 tcgtgttcac tgttgattta tctaaacctt tcaatgtttg aatcaacatt tctcgtgcag
     1861 cttcaaaatc gcgaatactg aacatcgttt gatgcgtatg gatataacgt cccactacgc
     1921 caatcactgt acttgggatt ccttcatttt gtgtatgagc agcacctgca tctgtaccgc
     1981 cttttgaaac aaagtattga taagggatgt tgtttgtttc agcgatatct agcaagtact
     2041 cacgcaaacg aggaagcatg atcaaacctg gatcttggat acgcatcaat gtaccttctc
     2101 ctaaatgtcc gaatgtgcca tttttcgtca ctgtgtcatc tgctgcagag cagtctactg
     2161 caaagaacag atctggcttg aatttattga cagaagcttt agaaccacgc aagcctactt
     2221 cttcttggac attcgcgcct gcaatcaatg tatgacctaa tgtttcattt tgtaacgctt
     2281 ctaaagcttc taataccatt gtacagccat agcggttgtc ccacgatttg ctgatgatat
     2341 tcttcccgtt ggctgttttg atcgtttcag cgtaaggaac gattgtgtct cctggaagaa
     2401 caccgtaact cattgcctct tctcttgatt caaacccagc atcgaacaaa atatctgtta
     2461 cttctactga tttttgtccg cttgttcctc gtaataaatg tgggggaacg gaagaagaaa
     2521 tacatggata attcttacca gtagaggttt taagtgtaaa acgttgagca gaaacaacgt
     2581 aagggttcca tccgcctaga ggtacgactt taaaaagccc gttttcttgg atttgtgtca
     2641 gcatgaatcc gacttcatcc atatgtgctg cgaccattac acgaggtgca tccgcttctt
     2701 catggtggcg cagcccaaaa atgccgccca aaccatctaa ctgtaactca tctaccagcg
     2761 gttccatatg actttccata taggcgcgaa cgtcttgttc aaaaccgctt gttccttgta
     2821 attctgttaa ctctttgatt cgttgaaatg ttttttcttc catatgctat tcgtctaaat
     2881 ttcagactaa actcctctca gacttatatt gatctgcgga taaaatatga ttttttctgc
     2941 agaccgatcg tatttattat agcttatttt ttctggtagc ggttatttat ttcaaagaaa
     3001 atatgagagt gtggtaaaat aaacacaaag agtagcaagt gagaagaagg aggtatatcc
     3061 acatgtatga agaaagcgaa ttaagctatt ttaaaggcgg gttagccgtt gggatcggtc
     3121 taggattatt gagtggggta gcttcaacga tttggtatca tcgcaaaaaa gtgttatcag
     3181 cagaccaagt gcttgaaaat atcaaaacat cttttttaaa agaaggcgca atcgaaggtg
     3241 cttggatctc ttttgaaaaa gaacctattc ggaaattcgc cgttcggtcg agtgcttatc
     3301 ggggtgggct ttcccggata gaagatggcg aaactgttta ttatgaattt ttagcagatg
     3361 ctcatacagg aaccgtatta gacatttcaa gaaaaaaggg cgcaatagat taatacgtca
     3421 aaaaaatgaa ataacgaata cgcacccact atctaaaaga gaggtatcct atccttcttt
     3481 tatagtgggt gatttttata aaaatagggc tatttcctct tggaatagct agtatttgta
     3541 gttattatac ggtattcttg aaataagatt agttttatta tataaggaga gtgacaaatg
     3601 gaatcacgtg aaaaaattgc cgtgctcatt ccttgttata acgaagaggc gacaatttca
     3661 actgttattg aagattttaa aagggaatta ccagaagcag atatttatgt ctatgataat
     3721 aattcgaccg atcgaacata cgaattggcc gttcaaggcg gagcaatcgt aaagaaagaa
     3781 ccacgtcaag gaaaagggaa tgtaatccgt caaatgtttt ttgatattga tgccgattat
     3841 tatttgatgg tagatggtga tgatacttat cctgcagaag ctgcacatgg tttgcttgag
     3901 aaattacgtt ctggcgaa
//
LOCUS       DACEXQ010000223         3913 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.223, whole genome shotgun sequence.
ACCESSION   DACEXQ010000223 DACEXQ010000000
VERSION     DACEXQ010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3913
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.223"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..121)
                     /locus_tag="IXH04_002439"
     CDS             complement(<1..121)
                     /locus_tag="IXH04_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HAQ1853003.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPA"
     gene            complement(485..3151)
                     /locus_tag="IXH04_002440"
     CDS             complement(485..3151)
                     /locus_tag="IXH04_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ1853004.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            3492..>3913
                     /locus_tag="IXH04_002441"
     CDS             3492..>3913
                     /locus_tag="IXH04_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ1853005.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSI"
BASE COUNT         1097 a          855 c          687 g         1274 t
ORIGIN      
        1 cagcaggcag gactttctca gttttcagat aattttttac atcttcagca atagttactt
       61 gcgattcttg ctggatttca taatattctt cagcaaattc atcccattct ttttcattca
      121 tttgatttcc tctaattttt tgttatatgt tttcactttt cggaataaat cattctcctc
      181 ttttatctat tctatctctt ttgatgaata ataaaatgat tggctttccg tctatcataa
      241 ataaaaaaag ggactgtgac caaagtcgtg tcaaagtccc tctttccgaa taaacagtgt
      301 ccaatcagat gaccctcagt attagctcaa aatgctgatt ttacaacttc ttccttaaac
      361 ggtgttctcc tgccaactct tcggtcacaa gtcacaatat tcaacaaaca tgagaagctg
      421 tttgttgaaa ttgcaccttg tgactcagaa ttaaacggca ggctcacaac ctcgttgatt
      481 cgttttattt atttccttgt actcgtacaa tgatcaattt agtgatttcc atcaaaacaa
      541 ttgtcacaag tgcgattccc atacatgtaa agacttctgt caatccaaat gctgatggga
      601 tagagaagac ttctttcagt cctgggatca ttgttaagca gtaaagcgcg ctacatacaa
      661 ctacagctag taagacactt ctgttagaga agaaccctgc ttggatagct gtttgtgtgt
      721 ttgaacgtgc tgggaatgtt tgcaatgtcc gacaccaaat caatgttgaa aatgacatgg
      781 ctacaccgaa ttcaggtgaa tgctgcatgc caagccattg agcaaagata acagcaatcc
      841 caatcagcac accacgatac gttacagaaa tcaatgtttt gtccgtaaag attcctgaat
      901 ttggatctct tggcgggcgg ttcatcgtag caggttcagc tttttctgta cctaatgcaa
      961 ttgctggcag tgaatcattg actaaattga tgaacaacag ttgtaatgct gtaaatggat
     1021 tggaccagtc agcaactaag gcaaagatga tcgcaatgat cgcacctaaa tttcctgcaa
     1081 atagataaga aatagctttc ttgatattat caaacacatt acgtcctact tctactgcgc
     1141 tgacaataga agcaaagtta tcatccgtca ataccatcgc tgctgcatct tttgccacat
     1201 ctgtacccgt tcccatagca attccgatgt ctgcttgttt caatgctggt gcgtcattta
     1261 caccatcacc tgtcatagct gagatatgac ctttattttg ccaagcacga acgatacgaa
     1321 ttttattctc aggtgaaaca cgtgcataaa cagtgacttt ttctaaaact tcgtttagtt
     1381 gatcttctgt caattcatct agttccgtcc cggtcaaagc caggtttcct tcttcaaaaa
     1441 tcccgatttg ttttgcgata gcaacagctg ttgttttgtg gtcaccagta atcatgattg
     1501 ttttgattcc tgcacttctt gcttctgtta ccgcctgagt cacttcagca cgtggaggat
     1561 cgatcatagc aagcaaacca acaaggatca aatcgtcttc atcttcaaat gataaatcaa
     1621 aattatcaag tggacggtaa gcaaaagcta gtacacgtaa tgcgcgtgtt gaaaaagctt
     1681 cattttgctc ttggaatttt ttcatcaatt catccgtcat cggaacgact tcaccattta
     1741 gcaatacttt tttactgcgt tggaagacga tatctggtcc gcctttaaca aacaagtaat
     1801 ttttgttatc aattgtgtgt ccagtagaca ttaattttcg atctgagtca aaaggaagtt
     1861 ctgcttgtct tgggtagttg tttcggattt cctgataagg ctggtttaat ttttcactaa
     1921 agtcgataaa cgctacttcc gtagggtcgc ccaatttagt accgtcttca ctgatcgatg
     1981 catcattcgc taaaacactg atttcgatca agcgacgttc gtcagctgtc cattttgaag
     2041 ggtcatttgt gaaatcccca gtatcgccat tacctaaaaa gtagtcaaca actgtcattt
     2101 tgttttgtgt caatgtacca gtcttatctg tacagatgat acttgtcgaa ccaagtgttt
     2161 caactgctgg aagcttccga ataatggcat gacgttttgc cattttattc gttcccaaag
     2221 ataacacgat tgttacgata gactgtaatg cttctggaat agcagctacg gctacggcta
     2281 cggcaaacat cactgcgctg acgatatctg cagtcatatc tcctgtacct tctccaagat
     2341 aaatacgaaa cagttggata cctaaaatca ataaagacaa tgccaaaata gccaaactta
     2401 gttttttact gaatttatcc agacctcgtt gtaacggagt gacttgctca gttgttgatt
     2461 ccaaaaggcc agcaacttgt ccgatctccg tattattccc agttgaagtc actaagaatt
     2521 taccgcgtcc gtaagttacg atcgttccac tgaataccat atttgtacga tctccgattg
     2581 gtaccgtact tgtgatatcc gttacttctt tttctgcaga agtcgactct cctgtcagca
     2641 taccttcatc tacttttaat gatcccgcat ctagtaaacg tccatcagca ggtacataat
     2701 cgcctgcgtc taacaacacg atatctccta ccacaagttc gtttgccgga atcgtttttt
     2761 ccttaccatc acgaatcact cgagcatcgg gcgctgaaag gtttttcaac gcttctaaag
     2821 aaccttctgc tttttttgtt tggacaacac tgattataga attcaacatc aatactgcaa
     2881 aaataactaa gaattcgatg tagtcaccca tgaccatctg aatcagtgcg acaatcagca
     2941 atacgataac cattgcatct ttgaaagttt ctaagaacaa tttccaagtt ggcttcttcg
     3001 gcttttcttt tagctggttc aagccatctc tttctaattt ttccttcgct tgcgtggtgc
     3061 tcaatccatc ggttgagctc tgcgtttgct gcatcaacga gtcgatcgtt tctttataga
     3121 ccgcttgttg tgcttgttcc ttgttttcca aatttcatcc cccatttttt gatcttggcc
     3181 ctagtcctgt ctgaaaaaaa agagacctaa gacaaatacg catttgtctt aggtctcacc
     3241 atttcataca ataccagaaa accatttggt taacttactg ttgatattat cgcaacaaac
     3301 gttgccggtt actcccctaa ggagatattc aactgtacta atatcctacc ttattaacct
     3361 acaaaaagca actactttag cccgatttac ctacattttc atgaaaggag tgaaaagtat
     3421 attcaccttg gtaggcttct ttcttctatc atgctatact aatgttattg tatgtaagga
     3481 ggaacatttt tgtggtatct agtattataa ccgcccttta tctattaaat gcacttattg
     3541 ctttggtggc tattttgatc aaaccccgag atgtagcagc catttgggca tggctcttag
     3601 tattgtttgc ccttcctggg gtgggctttg tattatattt atttttcgga cgtggattaa
     3661 cggataagaa aaaattctat ctccgacaaa gtgacttgaa agaattagaa aactttcagt
     3721 cttttagagg agataccatt gaacattacg atcctgacat gggcgataaa gacaaacagc
     3781 aatttgttga cttcttctct tcattaaatc gtatgccgct gacaagaatg aattctgtca
     3841 ctcttctcac agacggacaa gagaaattgg attcactgct tcaagatcta aaaaaagcca
     3901 aacattcgat cca
//
LOCUS       DACEXQ010000224         3906 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.224, whole genome shotgun sequence.
ACCESSION   DACEXQ010000224 DACEXQ010000000
VERSION     DACEXQ010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3906
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.224"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            90..308
                     /locus_tag="IXH04_002442"
     CDS             90..308
                     /locus_tag="IXH04_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853006.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(597..1109)
                     /locus_tag="IXH04_002443"
     CDS             complement(597..1109)
                     /locus_tag="IXH04_002443"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAQ1853007.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(1123..3420)
                     /gene="recJ"
                     /locus_tag="IXH04_002444"
     CDS             complement(1123..3420)
                     /gene="recJ"
                     /locus_tag="IXH04_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAQ1853008.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3562..>3906)
                     /locus_tag="IXH04_002445"
     CDS             complement(3562..>3906)
                     /locus_tag="IXH04_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAQ1853009.1"
                     /translation="SFGFTSFSAPLILVIIGSALIGALIVFLTASATLWQQKKQIKQL
                     TQEMSEYQTELQKKIEEAKEEQQREFRNERAELEAAYQAELQAKTAQISQLKAPEEQK
                     TQSPKQSFNYFD"
BASE COUNT         1079 a          865 c          689 g         1273 t
ORIGIN      
        1 tttatcactt ccctctttac aaaccgaaca aatattcgta tactaaatat acgaacaatt
       61 gttcgcataa attaaagagg tgagaattca tgaaatcaat gagacgtctt ggcttagtat
      121 taactgtttt gtttttaggt attattattg gtaatttcgg catacgtcca gctttacttg
      181 tgtttgttcc gttatttact ttgtggttca tgttgtggga tgaaaagaag taccgtcaat
      241 ctgagcggcg cagaattcat gaagatcatt tctatcatga gccctactac agatccaggc
      301 aatcataata aggattgact gctaattatt caaaagacaa gaagttaaac ttttacgggg
      361 gtgtgacaaa aatcggtcac atcctttttt tcgaataaac ggtgttctaa agccagtttt
      421 tcggtattaa gtcaaattgc ggaaaaagtg attttttaaa cttctactgg tctttgcttg
      481 tttccattta taaaaaaata gcgaccctca aaagtcagat agagaatctg acttttgagg
      541 gtcgctttaa aatcgcttct ttaagaataa catcaagaat agcttctttt ttctgcttaa
      601 tattccatta atgtaatgat gtcatatcct ttgattttgt cgcgtccatg gagatccatc
      661 aattcgatca aaaatgcgca gcctacgact acaccgccta attgttcgat cagttcgatc
      721 gtcgctttga ttgttcctcc agtagctagc aaatcgtcac aaatcagcac acgttgacct
      781 ggtttaatgg catccttatg caatgtcaat gtatctgttc catattctaa tccataagtc
      841 acttcgatgg tttctcgagg taacttccct tttttgcgga ctggggcaaa accgactcct
      901 aactcataag caacaggaca tcctacgatg aatccacggg cttctggccc aactaccata
      961 tcaattcgtt tttcttttgc ataatttacg atttgtttag tagcttcccg ataagcgtca
     1021 ccatctgcca tcagtgggga aatatctcga aacacaattc cttttgacgg atagtcggga
     1081 atactggcaa tataatcttt taaatccact ctttgtttcc tcctaggttg ttaaccattt
     1141 ctttatggta ggaatatcgc tcaaaagcaa aaattcctca actttgattt tattgagccg
     1201 tcgctggtat aatctgctct cagtcaaagg atgactttct gggctagcga ctttttgtaa
     1261 aacaccgttc tctattgtaa caaatttcaa ttcaaaaaac acctgtatca taaaaattaa
     1321 taatttttca gggatacgca aatattgagc tattgctttt attttatgac gtatatcaat
     1381 tcgttcttga gaatggatca atttaaacaa ttttgcgtac tgttctcttg tgcctacccc
     1441 attcaaatag gcctcatcag gtgaaatccc catcagataa acacgattga atgaataaga
     1501 ccgaaaaact tctttcagat ccgctaaatc atctggacaa tccataacaa ctagagagtt
     1561 tggttttctt ttttcaatta ttgcctttac agaatctagt ccttcgaaaa caatcgcagc
     1621 ttcctggata atctcaggca cgaatttcaa cgtcgaagaa tcaaatgcta aataaaggct
     1681 accttccgac gttcggctct gttcacggaa acgttttgcc cgccaatcaa atacttgcag
     1741 accacccaca gaaaaatcag ataccatcag ctgcggtttc ttacgcccgt tccattcatt
     1801 gatcgaaagc tttcctacca catctacctg attattcagc agttcttctt cttgagtacc
     1861 aaaaccaaaa gcgaccgcat caagctgact cgatgcatcg gaaaggacaa atttcagatg
     1921 ctgttggttt gctccgattc ttttgatatt ttctgcttgg acttgtcgaa ataaaaactg
     1981 aggcaaagga ttatccgtac caaatggcgc caatagtttc agctcatcaa ttcgctctac
     2041 tgttacttca tttggcagca atacttcatc aattctcaac tcaggccctc tcatcaagtc
     2101 aatttgatga tcgactatat attggttcat cttctcctgt agtatagtga cattttcact
     2161 tggcatcgtt aaaccgactg ctgcatgatg tcctccgaag taagtaaata aatctcgcat
     2221 ctggtcaagc atttcaaaaa gattcaaggc ttcaatacta cgtccagatc ctttagctga
     2281 tccatcttct tttaaagtaa ggacaagcgt tggtttaccc gtttcattca ttattttccc
     2341 agcaacaatg cccaaaacgc cttcatgcca tcctcggttt accaatagat ggatatgatt
     2401 ctccttattg accatcgcta atgcttcttg tgttatctgc tctacgattg cttttctttc
     2461 ttcattgatc tcattcaatt tttttgcttg aacggatgct tcctcttcat caaacgtcgc
     2521 aagtaacgag acggcaggac gtggatcgcc catccgaccg attgcattta gtctgggagc
     2581 aatcgaaaaa ccgatcgttg tttcgtctgc gtccctcatt tttacgccgc tttcttcaaa
     2641 tagcgcctga agtcctatcc gttctgaatg atgaatggca tcgattccta atgaaacaag
     2701 gattcgattc tcatcagtca atgaaactaa atcggcgatc gttcctatcg ctactaaatc
     2761 caaaaattct gcaggcggtt cttccaataa tgcagaagct actttgaatg ctactccgac
     2821 tcctgccaaa tctggaaatg gatagtgccc cgctggatga cgagggtgaa caatcgcata
     2881 agcgtctggt aaattctcag gtagttcgtg atgatctgta atgatcacgt ctacaccagc
     2941 ttgctgagcg tatgctaccg catcatttcc tgctaccccg ttatcaacag tgatgatcaa
     3001 ctggattcct tcttcgatct tttcttggta taccgattgg tttggtccat acccatgctc
     3061 aaatcgattt ggcaggaata cttctacttc tgcaccaagt aattccagtg tttctttcat
     3121 taccgttgca ctggtgatcc catctgcgtc ataatcaccg taaacgagga ttttttctcc
     3181 actgattaca gcttcttgga ttcggctgat tgctttatcc atatcgtgta tcagatacgg
     3241 atcatgtaat tcttgttcct ttggatgtaa aaatcggttg atatcctttt cctcatggta
     3301 acctctttgc cacagtaact taccgacaaa tggagataat ttcagttcac ggatcatttg
     3361 gagaaaggat tcagaaggct ctgtctcgct taacttccaa tcaaaatttg cttgtctcac
     3421 tttctcactc ctaacttagt tattatagca aaggaaatca gcaaaaaaaa gcagaaagaa
     3481 agaataaaaa cagatcagat ccgctagaca gatgttcagc aggacctgat cttttgtacg
     3541 accatttatg aaatatccct attagtcaaa ataattgaag ctttgtttgg gcgattgagt
     3601 tttttgttct tccggcgctt tcaattgact gatttgtgcg gttttcgctt gtaactccgc
     3661 ctgataagca gcttccaact ccgcacgttc gtttctaaac tcgcgttgct gttcttcttt
     3721 tgcttcttca attttctttt gaagttctgt ttgatattct gacatttcct gagtcagctg
     3781 tttgatttgc tttttttgct gccatagtgt tgccgaagca gtcaaaaata caatcagtgc
     3841 accaatcagc gcagaaccga tgatcactaa gatcaatggc gcagaaaagg aagtgaagcc
     3901 aaaact
//
LOCUS       DACEXQ010000225         3853 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.225, whole genome shotgun sequence.
ACCESSION   DACEXQ010000225 DACEXQ010000000
VERSION     DACEXQ010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3853
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.225"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(503..1060)
                     /locus_tag="IXH04_002446"
     CDS             complement(503..1060)
                     /locus_tag="IXH04_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853010.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1239..1850)
                     /locus_tag="IXH04_002447"
     CDS             complement(1239..1850)
                     /locus_tag="IXH04_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853011.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(1963..2220)
                     /locus_tag="IXH04_002448"
     CDS             complement(1963..2220)
                     /locus_tag="IXH04_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853012.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2817..3095)
                     /locus_tag="IXH04_002449"
     CDS             complement(2817..3095)
                     /locus_tag="IXH04_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853013.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3129..3380)
                     /locus_tag="IXH04_002450"
     CDS             complement(3129..3380)
                     /locus_tag="IXH04_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853014.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1173 a          784 c          509 g         1387 t
ORIGIN      
        1 aaattatctc caccaaataa tttattcata tttttgactt gcgcatacta actaattctt
       61 aaattttcat acaaaatttc tcaatttccc ttaacgagta atatttatgt acggtgattc
      121 tcactagtat cccaatcact ttttaagtta tcccgatttc aattctactt caaaaatagt
      181 tgcttttatg tatgctgtta gttgattaga tggtcatttg ttgagctagg tcgtttattt
      241 taaacaagtt gtttctttag cttataccta tctcgtctac caaacgcaag atggcaaaca
      301 caaatgcata cgcatctgta tttgccactt tgattcgtat aaagctaaca actattaact
      361 cctctcagct aatagttgct ctctttttta ttcagattca tttttgctga tcacagccta
      421 gtatttcaat ggttttgaac actaaaccaa attgttccat tacaacattc actggctgat
      481 gaaatggatt agtagatttc gattattcaa agcgattttc tattggtgga tcgtgttccc
      541 aactaataag acggattcca aacctatcgt acagatcttc taaaccactt ataaattttg
      601 cacctatttc aggtgtttta ttgcgtggat attctattgt tacgccatta aatggatacc
      661 aattaggatt aggctctctt tttttctgta catctcccat agaatagtta gcactttctg
      721 ggacataata tattcttcga ccatcctcta attcatctaa taagatattt cgtgatcgtc
      781 tatcacttgc taaatgtaga cgccacgtgc gataccatgc catgctacct gcatctgcag
      841 aaaatccatc aaataatgca acaattgtat cttctatcac aatcctttcc ccatctactt
      901 catattctaa tctaaaatca aactcccctc gggtaatttc tggtcttggt ggcttaggac
      961 ccaatattat tctacttaaa accgcattcg ttcccagatg aaacacagct ataacaacaa
     1021 taatgattaa tattttgttt ctatttgata aatttctcat tattcgcaca tcacctctac
     1081 attctcacat attagttcgt aatactcctt taatatcaaa atatctactg gtcgtaataa
     1141 tattcgctat gaattcctac tactcgtata agaaaggcca aatcatcagc ttttacttgc
     1201 tgatgatttg gcttgtttct atttatcgta attgttttct aatcaccaaa attgttaaca
     1261 atcggttcag atatttccca gttgattaag gtaattccgt attgtgtcaa aacttcttgc
     1321 gcttctccga aattaagtac agttcttatg tatccacttt ctcctaatat aaagtctacc
     1381 tctcttttaa aaccttcctc gagatttcta agggctatat gtggttttaa tgacattgat
     1441 tgtacatttt cccccatata atattgcgca cttcctcgac taaaatagat tcctagatcg
     1501 tccccaaaag gtagatcttc tctaccacta gctaatctac ttctccatct tcgttctctt
     1561 ttcatagaac ctgcgctgaa ctcacacccg ttaaactcag ctatgacagt atcttcaatt
     1621 atgttaatct gtccatctag ttcgtattca atcctaaatg gaaattctcc ataggtgatt
     1681 tccggttcgg gtattttagg tgagattaat gcaaagaaag gaaatattaa gcttggcaac
     1741 atcaaaaaaa ctcctcctat gatcacaatt cccgatgtaa tccaaaatat aatttgtaca
     1801 attctcattg atccattgta ttccatttta ttccccctat tactattcat atccgcctat
     1861 ctatatcgat cttccaatgc agagataatt gcttccatac cttgattttt tactgagtta
     1921 cctatgcgag atatcattct ccactaaggt gcatcatatt gtctatctaa catgattaat
     1981 tctccaatag gtgtatactc ctccaaaaac aacatttaat gcatctccct cgacaacata
     2041 agctaccatg tttgctgtat atgtgtttga gtctaattca taagcagata aatttactgt
     2101 cgctggatta aatataatcg cattcgtatt tatagctact gaatttgctg cttcagcacc
     2161 accttttgaa tgtccgatca ttgtgatttc aatagtagga tttacgtgga cagccctcac
     2221 cgtttaccct gaccattata gaaatcgctt acttttttga actacgcaac aaaggataca
     2281 atgctcctaa cttcactgtc tccatttttg tccactaagg aatataaaaa ataggcatta
     2341 attcatttaa ttcggtcaca acacgttacc ttttaaatca ttatactgta tctcatacat
     2401 tattatcaat aaactattat aaaaatgcaa aaaatgcttt atcaatctat tttgtaattg
     2461 gtcataatat cattgaaatc ataccctgta tcgatttttc ctatgaatct cgtctatttg
     2521 tgattgacga aaaagaggca agacagacac tggaatggta attttaccca ctattttgct
     2581 ccaactattt gtaatcgtac ccacttttga aataccgaga tgatcatttt aattgatatg
     2641 ctgattccca agcaaaaccc caagaaatta aatgctctat cgcatctagc ttcttgggtt
     2701 ttgcttatat aaattccaac atttaaattt tttatgctgt tcggtctcag aaatgccgtt
     2761 tttgcttata tcacaatatc aagatgtcaa attattcgtg ttgccgatga ctttgattaa
     2821 tttttttcaa attgtttatt aagactaatg ctgtcttttt gcagatacca atgttcgaac
     2881 tttttaacat tatcatatga tcctgcaagt tttaacttct taccattaga tgttatcaaa
     2941 gttataatta accttgcctt tttcccatta ggatctttta ttttatgaat tttctgcagg
     3001 taatctctcc taatggcagc tatttttacc tcatcagtgc tgataaacca ttgatcatcc
     3061 acatacatac tgcctgttat gttgtgtttt tccatttctt ccgagaacga aaagtttaag
     3121 gctcgttctt acttccaaag ttgaatgagt ccatggattg gacttagtgt gagaattgag
     3181 aacaacaata agaatccata tcctcccgca aacatcccta aggctatact gtctctccat
     3241 ccataagtag gattagaaga gaaaagagtc accgtttgta ataatatgaa aaggaataat
     3301 ccagcacaaa caccaaagat aacaactcca aacattgaat ttttatacaa tgaccattta
     3361 tttataggct tttctttcat tttacttccc cctactgttc tgttcacacg gaatgaattc
     3421 aaagttcata agccctcctc tttgaacata tgaatacgac ttgctaacta tactttaaaa
     3481 aagcttgttt taagcggtta taggcgagtt gtaatctcta ttcatctcat tagcaaataa
     3541 cttgccgaaa agtctactga aattaaagca gatagtggaa gaattattta gccaggatag
     3601 acactatttg tgaaacacat gtctttactt ttgaaattct atgatgaaaa gccttggaat
     3661 acagtagctc ttaattggta atactttttc cattataaat aacctctttt cattttatat
     3721 ttatcccctc taaaaataat atctaatcta atattttaaa cttatatttc cctcgctgtg
     3781 ctaatagagc attactaaat aaatagaaac tagaaaaaag cattacgcct gccagaataa
     3841 accaattatt tgt
//
LOCUS       DACEXQ010000226         3806 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.226, whole genome shotgun sequence.
ACCESSION   DACEXQ010000226 DACEXQ010000000
VERSION     DACEXQ010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3806
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.226"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            93..2192
                     /locus_tag="IXH04_002451"
     CDS             93..2192
                     /locus_tag="IXH04_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ1853015.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            2388..3251
                     /locus_tag="IXH04_002452"
     CDS             2388..3251
                     /locus_tag="IXH04_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ1853016.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            3356..>3806
                     /locus_tag="IXH04_002453"
     CDS             3356..>3806
                     /locus_tag="IXH04_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ1853017.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEK"
BASE COUNT         1520 a          636 c          772 g          878 t
ORIGIN      
        1 gtaaatccta aaaataagct attggcgtga cgtatagaat atgatagatt aaatggtagc
       61 gtaaacctag taaaggagcc gatcgctaag tgatgaaaaa atgtccaaat tgcggatcga
      121 aaaaaatgga gcagaatact atctgtccag actgcggtca tcaaataact gcagaagatc
      181 agcaaataaa agaatcaatt gaagaaacaa atacagatgc agctatgcag gataaatcaa
      241 ctgaagatgc agtctttcct gatacagaat taaacgatcc aatcgaatgg tcggaattga
      301 aagatttacc tctagaatct gtaatggaat tattcgaaaa agcagaacca gaagacgaag
      361 aaccacctgc tcacgatcaa gtaactaata tagagaaaaa tgaacagaag aaagcaactg
      421 ttcatgagac ttcggaagaa tcaggaaaga atcaagaaga aacgcttgaa tcaaaacaaa
      481 aaagaagagt agctgaactg aaagaaatcg tcgataatga ggaagaaaat tcgatactat
      541 ccgcttatat caaagcacat cgtgaagata caaaagaaga gcacgcgaaa gagctgctca
      601 aaatgatcag tgaaaaaatt gcacaggaaa acaaagtaga gccagaaaag gaacaagaaa
      661 ctgaaaaaga ctctgataga gaaaacgcca caggtaaaga aacagtaaaa ataaaagacg
      721 taaaaacgga tatcacagaa aaaactgaag tatttactga aaaacaatta gaaaaggaaa
      781 aaacgaataa actgccggaa gcagagaaaa aagaaatact cgaaaacaca gacaaaaagt
      841 caacagagcc cgatgaaata aatagtgaat ccgatgctga aaatcaatct gtaagcaaag
      901 agggtactga tcttgaagca aaaatagaaa aagaagagaa ccagcctaag ctgtttgaag
      961 cagaagcgaa atcaataaca atcgtgccag atactgaaaa aacacatcaa gaatcaaact
     1021 tagaaaaaac agatccagat aaagcgcaaa agaaaaaatc aaaaaaagta ccttatctcg
     1081 ttttagcagc agtacttcta ctcggtggag gaagctgggc ttattatgac catcatcaaa
     1141 aagtagaagc cgaagtagca gcagaagcaa aaagacagca acaaatggaa gaacttcatg
     1201 agaaacttgc tgccttttat acagatgatc accatcaatt tattcgtgca gatatgattc
     1261 atcaagattt atcaaaacta aaaaatgatt tatccagtct taagaatgaa aaaggatatt
     1321 cagagttaga aacaacttac caagatatcc aatctaagat ccgagatatc caaaaagtga
     1381 atgagtggtt catagcacct gccatcgtcg atgatcatct agcagatgac atcaagttga
     1441 aagcagataa agagatccaa gcaatcgaaa cggataacac agattttgga aaattgattc
     1501 aacaagcaca aacagaagct aaaaatcaat atgctgaatt gcaaacagct aaagaaaaaa
     1561 cagctatagt ctttaaagac ggaaaagtag tggattcagc aacacgcgac caatacaaga
     1621 cggcaaaaga agcagtagaa aaagtgaaaa atacagatct atctgctcag ttgaatgaac
     1681 aattgaaaca agtagataag atgctcactg ataaagagaa aaaagaagaa gaggaaaaga
     1741 aaaaagcaga agaagcgaaa aaagcagctg aagaacaagc aaaacaagcc gctcaagcag
     1801 cagctgcagc tcaagccgct caacaagctg cggtcgcaca agcagcacag caagaagcgg
     1861 catcctcgac cccttcctct tcaaactcag cgaatcagcc aatcaaatct gccagagcaa
     1921 gtgatattgc cgatgcatct aatccagcat ggacgtgggc accaggtgtc caaagcaaag
     1981 tgattgctac atgtatccaa cgtggatata tcgttgaagg aggttacaga ttagaaaaag
     2041 ccagaatcga aaatggggaa ggctattata atctatatgc cacttcaaca aaatctgcat
     2101 tgatgaaagg cattggagaa agtgctttgc cattttacat cgtaaccatc aactgtaaaa
     2161 ctggatggtt cggaggaaat gggagcaatt aattttaggc aaatgaaaga ggctgtgagc
     2221 ctgccgttta acaccgttta ttcgaaaaag gggagtatga cacaactgtt gtcatactcc
     2281 ttttttcacc tttccaaacc aaggctcagc tattcaagta gataacatta tgttatgata
     2341 gcaaagaaat gtattaagaa aacctttcac tatagaggga gaaaaaaatg aacgatttat
     2401 tagcaactat atttacaggt attattattg atgaaaatga aaaaaactac tttgtccaaa
     2461 aaaacggaca aacctttggc ttaagcaaat cagaaggtga acacaaaatc ggtgaagcag
     2521 ttgaaggatt cggttaccaa aatcaaaaac atgaaaatcg gatcactaca caaattccta
     2581 aaagccgtat cggtcattac gcttttggaa tcgttactgc tacacgaaaa gatttaggtg
     2641 cgtttgtcga tatcggtctt ccagataaag actttgtcgt atctcttgat gaattgccga
     2701 caatgacaga gctatggcct aaaaaagggg atcgcttgat gatttccctt agagttgatg
     2761 caaaagaccg tatttggggc agtttagcag atgaaaaaat attcaaatct ttaagcaaat
     2821 atggaacaga agaaatgaaa aacaaagatg tgataggaac agcataccgc ttgaaattaa
     2881 caggtacgta cgttttaacg gaagatttct atcttgggtt tatccatcct tctgaacgat
     2941 ataatgaacc tcgtctaggc gagcagataa aaggtcgagt gattggtgtc cgaccagacg
     3001 gtgtactgaa tctttcattg aagccaagag catatgaagc tatatcagat gatgcacaaa
     3061 tgattttaac cattttagaa cgttctgcag atcagcaaat caactttaca gataaatcgg
     3121 atccagaaga aatcatgaaa gcttttggta tcagcaaagg agcatttaag cgcgcattag
     3181 gtaatctttt gaaacaagga ttgattacac aagaaaacgg tgtgacaaaa ctagtcaaaa
     3241 aatctaattg agaatccctt gcatgttttt cttaactagc ttataataat tttaattaac
     3301 atagtgttat atcttttttt atttgtaatt attataaatt gttggggttg agtaaatggt
     3361 catgaatgct atttcggcga tgaaaaaaac aaaacagcag ctacatgaag cggggtttaa
     3421 gctgacaccg caaagagaag ctactctgct tgtgcttttg gaaaatgaga aagagcattt
     3481 atctgcagaa gaaatcttct ttttagtgaa aaaaaagagt ccggaaattg gacttgctac
     3541 tgtttatcga acccttgaaa tcttgacaga attgaaaatt gtcgataaag taagttttaa
     3601 tgatggatta gcccgttatg atttgagaaa agaaggcgcc aaacatttcc atcatcattt
     3661 actatgcttg aactgcggca cgatcgagga agtagaagag gatcttttac ttgaagtgga
     3721 gcaagtagta gaagaacgtt atcatttttt agtaaaagat catcggctaa cgttccatgg
     3781 aatctgtcag gaatgctatg aaaaag
//
LOCUS       DACEXQ010000227         3798 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.227, whole genome shotgun sequence.
ACCESSION   DACEXQ010000227 DACEXQ010000000
VERSION     DACEXQ010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.227"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(559..1599)
                     /locus_tag="IXH04_002454"
     CDS             complement(559..1599)
                     /locus_tag="IXH04_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAQ1853018.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(1948..2274)
                     /locus_tag="IXH04_002455"
     CDS             complement(1948..2274)
                     /locus_tag="IXH04_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853019.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2261..3049)
                     /locus_tag="IXH04_002456"
     CDS             complement(2261..3049)
                     /locus_tag="IXH04_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ1853020.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(3417..3707)
                     /locus_tag="IXH04_002457"
     CDS             complement(3417..3707)
                     /locus_tag="IXH04_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAQ1853021.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
BASE COUNT         1160 a          626 c          534 g         1478 t
ORIGIN      
        1 cgttgcgggt aataccgtaa cactgaaggg cacgcattaa ggcgtcatct taatgctttc
       61 ctgtaggaca ctgacggaga ttaccagtct cccgagcctt acaggggcct ttcttttttt
      121 tgtattttca attatcacaa tttatcgtcc aaaagtgcag aacgaaaatc aattcatcac
      181 aagcatatca gacaattaaa tttaaaacaa ctaaaaatat acaaaaaagt gtaattgaat
      241 attagcagca ctgcctgaaa agaagtaata tcaggctttt cagcttgggg aaataagtga
      301 attacctctc ttcatgaatt attataactc aaaaaagcac atatgcaaac actagttccc
      361 tatttttttg aatgaaaaat aactaaaata tctaacgatt atgactttaa aacctacgtt
      421 tgtgattgtt ttcataaact tttaatctaa aaactccgga ataatagcta tttatttgta
      481 acatttgtaa taaaattaaa atcataaaaa agttctaatt taaaattgat catttttata
      541 gtgatcgctt tttatcaact actctagcca attttccgta ctaactgttg gtaattcgtt
      601 aaatgtttct ctttgttcgt ttgcaagctt tgccgcaata gctttttcga caaaccaatt
      661 ttttacataa acgaaaatta gattctgcaa attgtttact ttttcagttt tttgtttttg
      721 ataagctttc agcaaagtca tatataatcc tttgtcagca tcaattccat aagattccag
      781 ttcttcataa acaacaactg tttgctctaa ttcttcagct tttttctttg cattatgaat
      841 agtttggacg gttttttgag cgtcttcgta agtactagaa aatgttttta tcaagtttaa
      901 tacattttct ggtacaaatg tatttatact gtcctctttc atgatgtcga cgaagttatc
      961 tagtaaaact tgattttgta actgatcagt ttcagtgtct attatgtgtc tattagtgtc
     1021 tatagaatta gaatagaata gattgtctgc gattttcgca attacggttg agtccttgat
     1081 atgactggat tttgccgtat ttgcgatttt cgcaattacg gtggtatcat tgatttctcg
     1141 cggttttgcc gtatttgcga ttttcgcaat tacggtggta tcagtattgt cttcctcttc
     1201 catgatttta aagacgtctt gattcgttac ttctggcttg cctaagtaca ataaattagg
     1261 catggattca atcttaccgt cacgtttatt tactcttcct ctcttctgtt tcagaagacc
     1321 tgctttttct aattcctttt tgattttaga tactttacct tcgcggcatt gtaacaattt
     1381 cattaattct tcaactgtga atatgaaaaa tatattatca tcagaatcta cccagttatt
     1441 tttgactgaa taatcaaatc gatctttaag tagcatataa gcaatttttg catcatttga
     1501 tagatccatg tatttttcgt tagtaaaaaa taccttaggc atttgataat atttttcacg
     1561 ataaatttct tttgatttgt aataattaaa atcattcata gccaatcctc tttctgtagg
     1621 atagcgtttt gttcaaaact atgttacaat gaattaaaca aatatatcta tccactatat
     1681 atttttctac tgctgataag tcgccaaact ttgttaaagc agtagtcttt ttttatcttt
     1741 ttttagttta ccttaaaaaa taaattaaag caaaccgctc tatttcaagg tttaagctca
     1801 tagtaactat atcacgatat tacaataata ttatatcacg atatcgtaat attacaatat
     1861 gaagataagc gacttatcag ctttgatact actgattttt ataaaaaaaa tatcacgata
     1921 ttatgatatc gtgatattga atgtaaatta aattttattt tgtttctttg ctagtttata
     1981 tccttcaatc aagtattctt caggtatgga ctttaatagc gcatcttcta taacgtctgg
     2041 tttacttttg tctgattcag caatagtcgt ccaatatttg agtgctgaca ttagaattgg
     2101 atttatacga atgctatctg ctgttcttac actacttcct tgatctttta tgactttact
     2161 tatatttttc ttggttaatt cgatttcttc tacttctttt gatttaaaat tctttttatt
     2221 tttatttcct agtaatctac ttactgcagc ttgactgcga ttatcttctg ccatcaaaat
     2281 caccaacttc ttttaaatat tcttctgtaa atgctttata gtcatcttca gctgttgagg
     2341 gtttattaaa attgattaga tctgatccag cagcttgcgc ttcgtcaacg gttattgtcc
     2401 tacgaatata agttttgaaa atttttgcac caatgactct agaaatttcg gttgctgcag
     2461 acgtcatttc tttagaaatg ttataacgct tattatactt agtaaataga actcctcgta
     2521 cttcaagctt gtcgttcgta tattctttta ccgtttcaat agtttcactt aactttatta
     2581 gtccttttaa tgagaaatac gcagcttcaa ctggaatgat aatttcatca gaaacagtaa
     2641 atgcgttgac tgtaagcatt cctaaacttg gtggacaatc aattataata taatcatagt
     2701 tgtccttgat tggttgaagt actcttttaa gcctgtgttc acttcctact tgagtaaatt
     2761 ccctctctgc agaagaaagg gctatgtcgg ctggcaaaac atcaattcct ttatagtcca
     2821 gaatagcatc tgtaatgtca gcctcccctt ttaatacttc ataaattgta ggttggtttg
     2881 ttccatctat atcgaaacct acattatctg tagcatttcc ctgaggatca atgtccacta
     2941 aaagtacatt tttaccgtaa acaaatttca aacatgctgc taaattaacg ctagtcgtag
     3001 ttttagaaac tccacctttt tgattggcta ctgacaatat tcttgccatt aaaatatccc
     3061 ccattcatct tatattataa tataattata tcataatatt gtgatattgc aatatcgtat
     3121 atgcattata ttgtaatatt gtgtgattat tttgttttaa agatatatat cggttcggat
     3181 ataaagatta ttcatatgtt attcgaaatt aaaataatca ctttttatta ctttaaaatt
     3241 ttttattaat gataagaaat atgttttgcg agcagcaact agaataattt aaacattact
     3301 aaactgtcta tattgttact aataattccc ttcttatagt gcattttctt agaaaaaaac
     3361 aatattacga tatcatgata ttaagatatc gtaatattat acaattgtga ttgatattat
     3421 atatgttttt ttattttttt atcatgttta atcttgaagg aatcatattc tttttctaaa
     3481 gacaggagct tttcataaac atcagacatg tttaaactag aggccaggct taaatcataa
     3541 acgaatgaag caggtaatga ttttattcta cgttgtcggg ttatccagct tgcaaccgtt
     3601 ccttgtgggt aggcatataa ctcacaaaat tgttctacag tgattcctaa attcttgata
     3661 atgtaaacat tgatcgggtg cgagtaaaca aatgtattga tagacatggt gcgtaagcct
     3721 actttctttt attattgcgt ttgcaataat agtttaacac acgcattatt ctagtatcta
     3781 ctcctatttg taaaaaca
//
LOCUS       DACEXQ010000228         3785 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.228, whole genome shotgun sequence.
ACCESSION   DACEXQ010000228 DACEXQ010000000
VERSION     DACEXQ010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.228"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..171
                     /locus_tag="IXH04_002458"
     CDS             <1..171
                     /locus_tag="IXH04_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ1853022.1"
                     /translation="SLFPLYHGSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFK
                     IEYTKKRQRLAY"
     gene            346..642
                     /locus_tag="IXH04_002459"
     CDS             346..642
                     /locus_tag="IXH04_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853023.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            649..2601
                     /locus_tag="IXH04_002460"
     CDS             649..2601
                     /locus_tag="IXH04_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAQ1853024.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2673..3170)
                     /gene="dfrG"
                     /locus_tag="IXH04_002461"
     CDS             complement(2673..3170)
                     /gene="dfrG"
                     /locus_tag="IXH04_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAQ1853025.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            3400..>3785
                     /locus_tag="IXH04_002462"
     CDS             3400..>3785
                     /locus_tag="IXH04_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ1853026.1"
                     /translation="MKYILSFCTPKLNYLLAYIRLYSGVLHLRDSVRVSEKEKIKVTE
                     MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRKKIENPHPLLQTTVE
                     PSKPEQREMLLDALLEISDSDPLLRY"
BASE COUNT         1344 a          614 c          655 g         1172 t
ORIGIN      
        1 tctctgttcc ctctttatca tggaagtgca aaaagtaata tagggattga taaccttata
       61 gaagttatta ctaataaatt ttattcatca acacatcgag gtccgtctga actttgcgga
      121 aatgttttca aaattgaata tacaaaaaaa agacaacgtc ttgcatatta agtagttcag
      181 ttttggagta caaaaagtga aaatgtaaac ctaaatgagt atcaaattgc aactgaattt
      241 gatactcgtt ttttattgtt aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag
      301 ttaaaatgta tataaaaatg cgaacgtgaa ataattattg taggaatgca aattggtgtg
      361 ttaaaatggt tacaatttac taagcaaaaa ggggtgcatt ttcctatgca atttcttgag
      421 ccaaaaaata agaacgctaa gtctgtggat tgggaaatat cagagcaagt aagggtgata
      481 gttaagcaat acgctgaata tgcagagcgt actgaaagcg aagcagtgga tgaattctta
      541 ttgaacattt tagacgataa gaagtttatt gaatggattg ccaacaagcg aagtaataag
      601 cggattgttg aaaaaatggg tatcaaagat agagtgggtt gatagacaat gaccaagctt
      661 aaaagattag ccacgaagga agatgaaatt gtagatgtta aaattccgat ttcaaacgaa
      721 gaattaaaag atagggctaa acaatatgat ttattaactc caaaacgatt tgcaacaagg
      781 tataacaaaa tgttgttctt gccaacgtct tttaaatgga acggttctga atatcccatt
      841 caatataatt actgcatcaa tccgttttgt tgtaattttg ggaaagaaca gcacaaattc
      901 aaagatgtaa aagggaaacc aagtcgatat aaaatgactg gtagttccaa agataaaggt
      961 cataaaggta tgtactgcaa tgacaatcct attggcagag gtgtatcgca aaactgtaca
     1021 gtaacccctt tatctaattg gtctgttgtt gaagaaatta aacgccttat agaaatcaat
     1081 agcatccaag atgttgagcc agattatcaa tttcataaag aaggctgctc ggaagaagaa
     1141 tcaaccccat tcaatgagcc gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct
     1201 caacgctatc aatgtaaggc gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa
     1261 accactactt atcaccaaca aaagaacacc attcttccaa tgttcgctaa gatggttgtt
     1321 ggaagggttt cagtcagtcg tacttgtgat attttaggaa ttggagttgg aacctactat
     1381 cataagctgg agtggctgta taggcgttgt ttggagtttt tagaacgata tgagacacaa
     1441 ccccttcaaa caaagaaatt taacgagatg tggcttaata cggacaagat gcactactac
     1501 ctaaataacg tccgtaagaa aggacaaggt tcaaagaaat atacggggtt tgaagacctt
     1561 aatatgcaaa cgtatatagt tgtatctgct gaggtcttat caagatatgt gttccgttct
     1621 gatgttgcct atgattggaa tatttcaatg gatgaattaa atgaagatac aagaaaattc
     1681 aaagaagacc atctcaatac ttttagtcgt aaaaatgata ggttagattg gtcttattat
     1741 ccacaagaac catccgctaa cgatagcgaa aataggaatg cttatcttca cgaattaggg
     1801 aaaattacta ataggagtag atttgtcgat ggtttaaatg ttgatgctcc ttatactaca
     1861 actgctcact attggttaat taaacaaatg gttaatgccg acgaatggcg aatgattagt
     1921 gatgatgact tctctattag aaacgccttt tatagagtat tcacgaaaga attaaggttg
     1981 agcgatgcac atcattttat ttgccaagtt aacaagacga aaagcagaaa acaatgctta
     2041 aaagaattcg gacaagcaaa agcagaactg ctcgattggg gagatatcag aggattcaaa
     2101 acaaagtttc tacggacttt ggcatctcat tatctaactg aattactaac aagtcatcaa
     2161 ttccacgaag aggctattag taaagatgga gaaagatatc gcaaatatgc cgataaccct
     2221 atcaaacatc cattagccac taaggataaa ggattttatt cagttgattg tagaacggat
     2281 ttatctgctc tggaaccaaa cgaaatcgca aagatgctat taaatgttaa tgaccattcg
     2341 accaatagct ttattcaaca gataagacgt tatatatcat ctcttgaaag accattaaca
     2401 acagctcgtg gtgataagaa gagctatatt tacgctaact tcaatccgaa gtatgctcaa
     2461 tttgcgatta caattctaag aacttactac aacttttgtc gcccttttaa atcagcggat
     2521 aaaaaggttt tgacacctgc tcaaagatta gggattacag ataaacaatt tgattggaaa
     2581 gatataattt atttcaaatg acaaaaagag cttggagtaa tcccaagctc ttgtgcttta
     2641 tctttactgt tcttcttcaa ttaacgcacc cgttaactca ataagttttt tctttcatat
     2701 acatgaaaat agtagttata cggattttta tcattcttta tccctttttg ggcaaatacc
     2761 tcattccatt cctcataatt cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt
     2821 atttttgtga tgtacatttt ctcaacataa gggaaaaaca aattataaat ctgttctcct
     2881 ccgaaaataa aaatttcttc ttcgttttta cataactcaa aaacatcttc tattgaatga
     2941 acaatttcac aaccattaaa ggtaaacccc ttatctctcg tcagaataat atttcttctg
     3001 tcaggtaagg ctcttccgat tgattcaagg ttcttcctac ctaatattat cggatgtccc
     3061 tttgtagtat ttttaacata ttcccagtcc ttgggaatcc tccaaggaat gtcattctct
     3121 ttgccaatca ctctattctt atccatcgca gcaatcaaag aaactttcat ttataacaca
     3181 gcctttttaa ctaatttacc atctaatttc ttatttctat caaaaaacct tttctcatta
     3241 tcctgcccgt tagttgaata agcaattttt tctataagtt atattatcat aataccacaa
     3301 aatcacaaat tttcattcaa ttatcatttt caattgcatt ttataaatca ttttgtatgt
     3361 aatttggtat gtaagttaaa atgaaaaaag tagtccattt tgaagtacat tttgtcattt
     3421 tgtactccaa aactgaacta cttacttgca tatatacgcc tttatagtgg agtactacat
     3481 ttacgagatt cggttagagt atcagaaaaa gaaaaaataa aagttacaga aatgtatact
     3541 tcaataaatg gtgaattatg taagattgat agagcttatt ctggagaaat tgttattttg
     3601 caaaatgagt ttttgaagtt aaatagtgtt cttggagata caaaactatt gccacagaga
     3661 aaaaagattg aaaatccgca ccctctacta caaacaactg ttgaaccgag taaacctgaa
     3721 cagagagaaa tgttgcttga tgcccttttg gaaatctcag atagtgatcc gcttctacga
     3781 tatta
//
LOCUS       DACEXQ010000229         3746 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.229, whole genome shotgun sequence.
ACCESSION   DACEXQ010000229 DACEXQ010000000
VERSION     DACEXQ010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.229"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..532)
                     /locus_tag="IXH04_002463"
     CDS             complement(<1..532)
                     /locus_tag="IXH04_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1853027.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQ"
     gene            complement(550..1383)
                     /locus_tag="IXH04_002464"
     CDS             complement(550..1383)
                     /locus_tag="IXH04_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1853028.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            complement(1397..2194)
                     /locus_tag="IXH04_002465"
     CDS             complement(1397..2194)
                     /locus_tag="IXH04_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1853029.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            complement(2227..2511)
                     /locus_tag="IXH04_002466"
     CDS             complement(2227..2511)
                     /locus_tag="IXH04_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1853030.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            complement(2508..3509)
                     /locus_tag="IXH04_002467"
     CDS             complement(2508..3509)
                     /locus_tag="IXH04_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ1853031.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            complement(3511..>3746)
                     /locus_tag="IXH04_002468"
     CDS             complement(3511..>3746)
                     /locus_tag="IXH04_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="similar to full-length Gnd, these proteins seems to
                     have a truncated C-terminal 6PGD domainin; in
                     Methylobacillus flagellatus this gene is essential for
                     NAD+-dependent oxidation of 6-phosphogluconate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase
                     (decarboxylating)"
                     /protein_id="HAQ1853032.1"
                     /translation="DQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLF
                     MRNLSQEEDSFSAKVVSALRNGFGGHEIVEKGE"
BASE COUNT         1079 a          855 c          626 g         1186 t
ORIGIN      
        1 cttgtcttgc gaaaaatacc cctagtaaaa cctgatacaa cccaaagcag ataaaaggaa
       61 aaagtgcgcc taaaggactt ccctgttgtg cccaaggtac cgcgattgcg ataaaaatat
      121 attggacggt tcctgaatcg attgcatctc cgattcctgc tagtgccccc atcaatcccg
      181 ctttcgtgtt atacatcaag tcatcctgca tcaagatctc tttttcttca tactcttctt
      241 ctgctctttg ctgttccata gaagacatta ggccagtaat cacaccgcca ccccagctta
      301 attgagtgtt gaagaacagt aactgccgtt tatatgctgc ttttaaagag tcttcatctt
      361 tataaagttt tttcaagatt ggcatcattc cgtatagtag tgatggcgcc aagtaacgtt
      421 caaaagagtg aggaatttcg tttgcaaaat gccatctcag ataggctttt gttacatctt
      481 ttttcgtgat ttcttcagga gcacctgttt gattatccat tatttcagcc atttcccatt
      541 cctcctaaat taaaatccgt catcatagtc atcttcgtca tcaaaagatg ctgaattgtt
      601 attgcttgtg gcaggctgta cttgtttccc tttttgcgac tggacaaaga tcaaagcgat
      661 aataatccca aaaatggcat aagtaaccat cgtaatatct aaagatttca aaacaacact
      721 cataaaataa gcaaggaaga aaaagaccag ataatctttt cgaccgataa cgtatacagt
      781 tgtcgcgata ccgattgccg ctaaccctcc acctactact tcaagaatgt ggataacgac
      841 tgttccctct aaagctttga ttacatcagc gatcaatggc gcccctaaat ataaagcaat
      901 gaaaacaagc ggaacgaata ataaaaatga ggcaaccgtt ggataaagaa taattgatcg
      961 ggccatagct tttgtattca aatcttctac agctttgtct gtatatttac ctaaaaaagt
     1021 attgatgaaa aaacgaaact gatacaagta acttccgagc aatccgactg gcaccgcaac
     1081 ggcaatcgct gcttctggcg acagatcccc taataatgcg actggtactg cgatagctgc
     1141 tgcaatagat ggttcagctg gcattgatcc tcctggagaa aaaaccccca tataaatcat
     1201 ttgcaaagcg gctgtaacaa tcatcgcttg tgcaacttcc cccatgatca acccgacgac
     1261 taatccagtc atcaaaggag aaaaacgtaa ggtcaacgtg gctcctccac ctaaccagcg
     1321 ggacataacc gcggctaccc ataaagcaat caacaaactt tgtactacac ttaatgtttc
     1381 catgtgtcct cctccttcag atctttctga tataaccttc cagttccatc attgactgta
     1441 tcagtgattt ctgtgttacc tctataggaa tcaaatgcac tttctctttc gaatcaataa
     1501 attcagccat tttatctatt atctgtttat ctttcggaaa taatcccatt tgttctagag
     1561 aaaccggcag ttctaattcc cgatacaaag gatataactc gtctattttt tcccagcgac
     1621 cttctagtgc cagttgatag aaaatcccat acgctacttt ttctccatgt agaaaacgat
     1681 ggctttccgg aatatattta ctgatagcgt catgcatagc atgtgctgcc acatttcttg
     1741 cgtatttatc tcctaatcct ccaactaaac cagcaacagc aaagataatc tctgaaagct
     1801 gtttgaactc cgatgtcagt tttctttgtg ccatatcttg gattgcttta gcagaatgat
     1861 tcaaaatctc ttctttgcag atcttcgcag tatcttgtgc tagcttcaaa aatgattcat
     1921 cttgcaaaaa atcttgttct aagatgagct cagattcata ccattttgct agtgtatctg
     1981 ctaacccggc aatgaaatat ggaacagggg aatcaatcac taattgggga tctgttataa
     2041 aaaaagcagc ttgtctgtaa aagtgttctg ttttcccttc tgcttgtcca ttctccttat
     2101 acatgacagc aagaggtgtc catggagcac aattactagc taaagtaggg atgactccga
     2161 aatcgattct tgcaagattg gcactataac ccactagatc ggtcagtttt cctccgccaa
     2221 cgccaactac gaaatcgatt tcattttttt gatgattgta gctattcgtc tggcttcagc
     2281 ataactacat tctccagaat aacgatgata aaaaaattga atcgtttcgt cattcaaaaa
     2341 tactaattta ggtagcgcct tttcccatga taccgttccg tgggtgacca gtactctttt
     2401 cgcgccgtat gatttcaaga gatcagaaac ttttgaaata ccaccttctt catataaata
     2461 aaattgaggg cctgttttca cttttaaacc gttcaccatt ttcttcatca tccttttata
     2521 tagttgctct ttgtctggac agaacgttca ggaagaaggt gctgactgat ctgttcgaca
     2581 tctctgacac atttttcacc cattgcttct aaacattccc ttgtgtaggc cgatatatgt
     2641 ggcgtaatca ctacttgttc aaacgctaaa tacggatgat cttgacgtcc cggttcttct
     2701 tctaaaacat ctgtggcata tcctgcgatc tttccgcttt tcagaccatt aatcatcgct
     2761 tcttcatcaa ctagagcgcc tcttgcacta ttagatagat aaacattttc tttcatctga
     2821 tcgacttctt tatcagaaat catgtgatag ttctcttctg tcaaattcgc acataaacaa
     2881 atgatatctg attgttccag taattcttga agttctactt tttttgcacc aaagctttcg
     2941 atgtataaat ctgatttgta aggatcataa gctagaacat cacagcgaaa accgttacgc
     3001 aaaatttcga ttactccgct tccggtattt cctgcaccaa tcacaccaac tgttttgtta
     3061 aacagcgtcc taccgacgaa ctccgcccga tcttcccatc gatcgtcctt gactcttgct
     3121 tgtgcttgtg tcgtgcgtcg aagcatggac aacaaattag ttatattatt ttctgctaca
     3181 gcatttcgtt caactaaagc aggtatgata gatacgattg tctggtgttc ttttgctgct
     3241 tttaaatcaa tattgttgta accaatacca tgtcgggaaa tcaatagcag ttcatctttg
     3301 ttttcaaaaa aatctcgtcg aaaaaatggc gtaacacttg caataatgat attgaatcct
     3361 ttaagtaact ccgctagttc ttttcctgga atctcttgat caacagtaaa gaaatgaacg
     3421 tctccaactg actttaatct ttccaagtgg tgtggaaaga ctcttccgaa actgctggaa
     3481 ttaacgattg ctatatgata atctggcatt ttactcaccc ttttcaacta tttcatgtcc
     3541 gccaaaccca tttcttagtg ctgaaactac ttttgctgaa aaactatctt cttcctgtga
     3601 aagattcctc atgaacaaac tagttgcaat tactggaact ggaacagcca tttctagcgc
     3661 tgtttcaacc gtccattttg cttcacctga tgcagaaacg attccccgat aattacttaa
     3721 attgggatgt tcgtttaact gatctt
//
LOCUS       DACEXQ010000230         3684 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.230, whole genome shotgun sequence.
ACCESSION   DACEXQ010000230 DACEXQ010000000
VERSION     DACEXQ010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3684
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.230"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(326..688)
                     /locus_tag="IXH04_002469"
     CDS             complement(326..688)
                     /locus_tag="IXH04_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853033.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(692..1000)
                     /locus_tag="IXH04_002470"
     CDS             complement(692..1000)
                     /locus_tag="IXH04_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853034.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERMMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM
                     "
     gene            complement(1009..1428)
                     /locus_tag="IXH04_002471"
     CDS             complement(1009..1428)
                     /locus_tag="IXH04_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853035.1"
                     /translation="MTDLPEKMTIKNLADELGISKNTVRNQLVNAGYDLARYKDNGVI
                     MLDKELIGVVRSIYRSKGGQLTGDLPVNDAGNDFPLEKENEFLREQLAEKDRHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(1421..1654)
                     /locus_tag="IXH04_002472"
     CDS             complement(1421..1654)
                     /locus_tag="IXH04_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853036.1"
                     /translation="MAEKQKHFGKKNWLVNLTGDNVVVVFLDSKKMSGKLLDVTQYEL
                     ILKNNKIDSPIIIPKHAVKYVYGVAESNGEDND"
     gene            complement(1657..2625)
                     /locus_tag="IXH04_002473"
     CDS             complement(1657..2625)
                     /locus_tag="IXH04_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAQ1853037.1"
                     /translation="MSKNGIIVNEWENLTALSNRRLNVQNMYTPLKMQWSIDRITIVG
                     KLKENIYYHTQNDVLILDFEQLMRVNEGNGYLKAVGNNGWQLLDQYEENIAYIEILKW
                     QDGKGRIDFNPNKIGHFLASSMKNFIHDLFLEPHFSRADIACDIIDVPDEFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRLYNKKLEQETKRKIVPPEIETWWRLELQ
                     LRRGKATDWHAMVHESLNSFASPHFLPLDVKTNDKVMIFGLIANPEMWGFLERRMKYK
                     LRDILKRESQNDELTNHLRETFSESADVLKKELDTWLQGLDVTEEE"
     gene            complement(3072..>3684)
                     /locus_tag="IXH04_002474"
     CDS             complement(3072..>3684)
                     /locus_tag="IXH04_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAQ1853038.1"
                     /translation="ELRYISRQREENERDKALMASERAKIEEEKKNMLNVKKEMAVIF
                     QKDMVPLESFKRLPDGSYHLSGNDFVDLYQRATRATNAKRNADNMSEQYLQMLEQNTE
                     LNEYVATLEEERHLSGRKQLADLQVENKKLKEENRSLRQRLERLKNAFQKSITRLSIR
                     FGLAEKDMGLSEELEVLEQEKTLFYRQNVSQRKLERSEDEIEW"
BASE COUNT         1052 a          841 c          520 g         1271 t
ORIGIN      
        1 tgcttatgcc aatcattggt tttctggcga tcagttaact cacgattatt atgagaaata
       61 cttgtattct tcgttccttt tttaaatgaa atactaaacc ccaagttaaa accctccttc
      121 gcgctgtcgc taataaaaag attgtaaacc tttctgttac tctttgtcaa agagtaacat
      181 tttcctaatt gtgacatggc tgtcacaatt ccaaatgctc tccgagggta ttcgctgtca
      241 caagtgacaa cgaaaaaagg agccgaaagc gtgtcacgct ctcaactcct tttacgcttt
      301 attttgcttc acgcaaaata atcgcttatc gtaaattcac gtcattaaat attgacgtaa
      361 atttactctc actcatctga cttttcttat tcggtatcac tccgaataag ccgtcagatt
      421 catcgggata atctcgttcg cttggcgctg atttttcatc agcaagccac tcattatcca
      481 ctgatttttc gtgttgctga tcgtcagaaa tcgacgatag ccaccgctca aaatcagcaa
      541 aagtttcaaa cccaaaacgc ttgcgaagag ccttgcccat tgcagcattt tctttctcaa
      601 tcaagcgttt tttctgttgg cgaagtttcg cttgttcttt ttttaatttt tcttcttgcg
      661 ctttaatatc tgctaaagtc attcccataa actacatctc cttttctttg gaattaataa
      721 cggaaatttg gtttaactgc ataacatgac tcattaattt caaccaaaat tctttttctg
      781 attcatcagt tgtttgcgct aaatgcttat gaacaaattc attagtcaaa actgaagatt
      841 ttgctagatc agcattgata accgagttga gaacatccat cattcgttca agcatttcac
      901 tataattatc ttcatccggc aaattcaaat aatcagctaa ccgttcatac atcaattcaa
      961 ttctatcgac tgtttcttct ctcaaatcaa ttttatccat gtcacacctc acttccagaa
     1021 ttgccaccat tttcgatttt ttaattcttg attttctagt aacaactgat cgtttttttg
     1081 ttgctgaagc aaagtcaatt tttgttggtc acgaatcatt tccgttaatt cagaaatatg
     1141 cctatctttt tccgctaatt gttcacgcaa aaattcattt tctttttcta acgggaaatc
     1201 attgcctgca tcattgaccg gtaaatcacc agtcaattga ccaccttttg accgataaat
     1261 tgaccgcaca acaccaatca attccttgtc taacatgata accccattat ctttataccg
     1321 agcaaggtca taacctgcat tgaccagttg attgcgcact gtatttttag atatacccaa
     1381 ctcatcagca aggtttttta tggtcatttt ttctggcaaa tcagtcatta tcttctccgt
     1441 tactttcagc taccccataa acatatttaa cagcatgctt agggataatg attggggaat
     1501 caattttatt atttttcaaa atcaactcat actgcgttac atcaagtaac ttaccggaca
     1561 tttttttgct atccaaaaaa acaacgacaa cgttatcacc tgttaaattc accaaccaat
     1621 ttttcttacc aaaatgcttt tgtttttctg ccattcttat tcctcctccg ttacatctaa
     1681 cccttgaagc caagtgtcta attctttttt caaaacatct gctgattccg aaaatgtttc
     1741 tcgtaaatga ttcgtcaact catcattttg actttctctt ttcaaaatat ctctcaattt
     1801 gtatttcatt ctacgctcta aaaaccccca catttcaggg ttagcaatca gtccaaaaat
     1861 catcacctta tcatttgttt ttacatcaag tggtagaaaa tgagggctag caaaactatt
     1921 taaactttca tgaaccattg catgccaatc tgttgctttc ccacgcctta actgcaattc
     1981 cagtcgccac caagtttcta tttctggtgg cacaatttta cgcttggttt cctgttccaa
     2041 ttttttgttg tatagccgaa tctggcgttc gctggcacga ctgccccaat aagctgtttc
     2101 cagcttccca gaccgcccat aaattggctt gaaagaaaca ggatcaacca ctcgatattg
     2161 tgtaataaac tcatcaggca catcgataat atcgcaagca atatccgcac gtgagaaatg
     2221 tggttctaga aataaatcat gaatgaaatt tttcattgaa ctagctaaaa aatgaccaat
     2281 cttattcggg ttaaaatcaa tccgcccttt gccatcttgc catttcaaaa tctcaatata
     2341 agcaatattt tcttcgtatt ggtctaataa ttgccagccg ttattcccaa ccgcttttaa
     2401 atagccattg ccttcattca cccgcattaa ttgctcaaaa tctaaaatca acacatcatt
     2461 ttgagtgtga taataaatat tttccttcag ttttccaacg atagtaattc ggtcaattga
     2521 ccactgcatt tttaaggggg tgtacatatt ttgtacattt aaccgcctgt tagatagggc
     2581 tgttaaattt tcccactcat ttacgattat gccattttta ctcaaatttt gttcctcctc
     2641 aaagaaaaag agacttctgg aaagaatttt caaaagttca cgtaactctt aaaaattcat
     2701 taccaaaaaa tctctagtct tgaagaaatc taaaaatatt cgtataatga ttaagaagtt
     2761 acggagctag tgcgattagt ttccgtaaag gtcgagaacg ttggtagcgt tgctcgacta
     2821 tttttttact cttttttcag ttcatttgtt tgctaactca ataatagcac ttctctttag
     2881 ctatgcaaat atccaaaaaa taaaaccgca ccctttgaaa agggcgcccc caaacttcgc
     2941 taacgctagg ttggccaaaa aatggactgg ccatttttta gcaaatgtca gcttaatagg
     3001 ctagtggttt agccgtcaac tccttatcct cggcacaaac cgcctccggt accgttgact
     3061 gcacatcgta actaccactc gatctcatct tcagaacgct ctaatttacg ctgtgagacg
     3121 ttttgacgat aaaataaggt tttctcctgc tctaacactt ctaattcctc agaaagcccc
     3181 atatcctttt ctgcaagacc aaaacggata gacaaacgag tgatcgattt ttgaaatgcg
     3241 ttttttaatc gctctaaacg ctggcgtaaa gagcgatttt cttctttcag ctttttattt
     3301 tccacttgta aatctgctaa ttgttttcta ccagacaaat gacgctcttc ttccaatgta
     3361 gctacatact catttaattc tgtattttgc tcaagcatct gtaaatattg ctcactcatg
     3421 ttatcagcat ttctcttggc atttgttgct ctcgtagctc gttgatacaa atcaacaaaa
     3481 tcattcccag ataaatgata cgaaccatca ggaagcctct tgaaactttc taaaggaacc
     3541 atatcttttt ggaaaatgac cgccatctct ttttttacat tcagcatatt tttcttctct
     3601 tcttcaatct tcgctctctc agaagccatc agagccttat cacgctcgtt ttcttctctt
     3661 tgcctagaaa tatacctcaa ctca
//
LOCUS       DACEXQ010000231         3656 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.231, whole genome shotgun sequence.
ACCESSION   DACEXQ010000231 DACEXQ010000000
VERSION     DACEXQ010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.231"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(67..1269)
                     /locus_tag="IXH04_002475"
     CDS             complement(67..1269)
                     /locus_tag="IXH04_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853039.1"
                     /translation="MKLFKKIFFAFVLLIASYCVLLTITFFIPQHSIESNAEKSLQMV
                     EKEGMYPSINQGNMLGTRLDNFTDHLMIRKTKADPELNSLENAMSMADYPRYWHGYQL
                     FLRPLLLGMSLGSIRMIYAAILFLLIGLTSYFLTKCSDIYLTTALLISLAIGNAATFF
                     FSMQFSNIWILTLLSVLLFLTKPQLSNTNQQLFLYFFVIGSLANFFDLLTTPVISWGI
                     PVIIIYYVTNKYLMDKRSSLSKQVGSFVFTGIFWGLGYGLTWVAKWILSSIILNKNIV
                     KDAINQILFRTEGNDKYPLHRLEMLKSNIRLMYPKVAILLLLITCLVFLYFAYRKRNS
                     WRPFQLLVLFVLAAVTPYIWYNILANHSQIHYWFTYRTQIITSFAILSAFAFLIPPEK
                     SKDELNYF"
     gene            complement(1256..3043)
                     /locus_tag="IXH04_002476"
     CDS             complement(1256..3043)
                     /locus_tag="IXH04_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853040.1"
                     /translation="MHKKKENLFPTFFATLFASGFAFFTSYGLGTLNPTVIIGLAMVL
                     LLAFKQYGIELFSIKNKVAAIARLFFSAAFALTLSIGRQIQFQYVSANYTKTTIADGS
                     IFYFLVYFSIFVYSFLFLQSIYLSMPKLETFFTAQNTQAFSSRKYFLFFTVFLVLCWL
                     PYLLALYPGVVLPDSLSSVAQSMGDVPISNHHPVLFTLLVKLFVHDLPTDTINHGVFL
                     FSIFQTLITASAISYSLCWLLKRNIHFFPVLISLCYFAFAPVFPIYALNMQKDTLFSI
                     ACFLFSLAVFDFIFQREKPTILNQIYVIILALLTTYLRNNGIYVVTGTGLICGLIILK
                     QKRYQAFFQVVLGYTVIAGLLIHPLMARYAIPTETAEQLGVPLQQISRTIVMDGDLSK
                     KDKAFMNQLLPIKDYEAYSPSLADSIKWHKNFNNEFLNTHKKEFLSLWARNLPSNIKI
                     YTDAYILETFGFWVPGTKNSYGFLDTRVNTNNYGMKQVDLIEHLTGLSGMKKIIDARD
                     FFGSGTLLWLAIGSALLAIFKKKYRYLLLFLPGMLVFATIMIATPVAFSLRYVFVLAL
                     GLPVYILSPFLLDKREKMKTDIGDTHETI"
     gene            complement(3056..>3656)
                     /locus_tag="IXH04_002477"
     CDS             complement(3056..>3656)
                     /locus_tag="IXH04_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAQ1853041.1"
                     /translation="GTYFDENKRPFHDFGNNLVKNTITYLYKTKIRDVMTGYRGFNRI
                     FVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKVIM
                     MIIKMCKDYKPLLFFGIWTIFFFLFGLVMGIPVIREYMLTSFITRIPSAILSTGLMTL
                     ALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYQNKEMK"
BASE COUNT         1330 a          634 c          639 g         1053 t
ORIGIN      
        1 aaaactccgg ctatctctga cagctggagt ttttatgttt aaaaatgaat tagtacatcg
       61 gtattcttaa aagtaattta gttcgtcttt tgatttctca ggagggatca aaaaggcgaa
      121 agcactaagt atagcaaaac ttgtgatgat ttgcgtacga tacgtaaacc aataatgaat
      181 ctgtgagtga ttagctaaaa tattgtacca aatatacggc gttacagcag ctaaaacaaa
      241 caaaactaga agttggaacg gacgccagct atttcgttta cgataagcaa aatagagaaa
      301 aacaagacaa gtaatcaata gcaataatat tgctactttt ggatacatca atctaatatt
      361 gcttttcagc atctctaatc gatgcaacgg gtatttgtcg tttccttccg ttcgaaagag
      421 gatttggttg attgcatctt ttacaatgtt tttgttcaat atgatcgatg ataggatcca
      481 tttagccacc catgtcaaac catagcctaa tccccaaaag ataccggtaa aaacaaaact
      541 gcctacttgt ttgctcaatg aactgcgttt atccattaaa tacttatttg ttacataata
      601 aataatgatc acaggtatcc cccaagagat gaccggtgtt gtcaacaagt caaaaaagtt
      661 agctaaagaa ccaatgacaa agaaatacaa gaataattgt tggttggtgt tggagagttg
      721 tggttttgtt aaaaatagta atacagacaa taaagtcaaa atccaaatat ttgaaaattg
      781 catactgaag aaaaaggttg cagcatttcc tatagcaagc gaaatcaaca aggctgtcgt
      841 taaatagatg tcgctacact tggttaaaaa gtagctcgtc aatccaatca gcagaaaaag
      901 aatggctgcg taaatcattc gaatactccc caaactcatc cctagcagta aaggtcgtaa
      961 aaaaagctga taaccatgcc aataacgggg ataatccgcc atagacatcg cgttctccaa
     1021 ggagttcaat tcaggatctg cttttgtttt tcgtatcatc aaatgatcag taaaattatc
     1081 taaccgagtg cctagcatat ttccttgatt gatagaagga tacattcctt ctttttcaac
     1141 catctgtaga gatttttctg cattcgattc aatagagtgc tgcggaatga aaaaagtaat
     1201 tgtcagtagt acgcaatagc tagcgatcaa gagaacaaaa gcaaaaaata tctttttaaa
     1261 tagtttcatg agtatctcct atatctgttt tcattttttc tcttttatct agtaaaaaag
     1321 gagagaggat ataaacaggt agacctaatg ctaaaacaaa cacataccgt aaactaaaag
     1381 ccacaggagt agcaatcata atagtagcaa aaacaagcat accgggaaga aaaaggagta
     1441 aataccgata ttttttctta aagattgcta aaagtgcact gccgatagct aaccacagta
     1501 atgtgccgga accaaagaaa tcacgagcat cgatgatttt tttcattcct gacaatccag
     1561 taagatgttc aatcaaatct acttgcttca taccataatt attcgtgttt acacgagtat
     1621 ctaaaaagcc gtaactattt tttgtaccag gtacccagaa cccgaacgtt tctaaaatat
     1681 aagcatctgt atagattttt atattacttg gtaaatttct tgcccatagt gatagaaatt
     1741 cttttttatg tgtattcaaa aactcattgt taaaattttt atgccacttg attgagtctg
     1801 caagactagg agaatacgct tcataatctt ttatcgggag tagctgattc atgaatgctt
     1861 tatctttctt agacaagtca ccatccataa caatagttct agaaatttgc tgcaaaggaa
     1921 cacctaattg ttcagcagtt tctgtaggaa tagcataacg agccatcaac gggtggatga
     1981 gtagtccagc aatcactgta taacccagca ccacttgaaa gaatgcttga tacctttttt
     2041 gtttcaaaat aatcaagccg caaatcaaac ctgtgccggt tactacataa atcccattat
     2101 ttcttagata ggtggtcagt aaagcaagga tgattacgta aatctgattg agaatcgttg
     2161 gcttttctcg ttgaaaaata aagtcaaaaa ccgctaagga aaataaaaag caagcgatag
     2221 agaacaaagt atctttttgc atgttcaagg cgtagattgg aaaaaccggt gcaaatgcaa
     2281 aatagcataa actaatgagt accggaaaaa aatggatgtt tctttttaac agccagcaaa
     2341 gagaataact tatcgctgaa gctgttatca atgtttgaaa aatcgaaaat aaaaatacac
     2401 cgtgattgat cgtatcagtt ggtaaatcat gaacaaatag cttgaccagt aaggtgaata
     2461 agactggatg atgattgctt ataggaacat cacccataga ttgggcgacc gaacttaacg
     2521 aatcaggcaa aaccactcca ggatacaatg ccaacagata aggaagccaa cacagaacta
     2581 aaaagacagt aaagaataaa aaatattttc ttgaagagaa agcctgtgtg ttttgagcag
     2641 taaaaaatgt ttccagtttt ggcattgata aataaatcga ttgtaaaaat aaaaaagaat
     2701 acacaaaaat ggagaaataa acaagaaagt aaaaaatact tccatcagca atcgttgtct
     2761 ttgtatagtt tgctgagaca tactgaaatt gtatctgtct gccaatagaa agtgtaagag
     2821 caaaagctgc agaaaaaaac aatcgggcaa tagctgcaac tttgtttttt attgaaaaca
     2881 actctattcc atattgctta aaagcaagta gcaaaaccat ggctaaacca atgattactg
     2941 ttggattcaa agttcctaga ccataagaag taaaaaaagc aaaacctgac gcaaataaag
     3001 ttgcaaaaaa agtaggaaat agattctcct ttttcttatg cataaaaaag gcctcctact
     3061 tcatttcttt attttgatat tcgtggtaga catggtataa attcaattca tattctttct
     3121 tggcatttgt gacgaccgta tccaaaatca accctgttac taatgaaaga agggctaaag
     3181 tcatcaatcc agtacttaaa atcgcagaag ggatgcgtgt aataaagctt gtcagcatat
     3241 attcacgaat cacaggaatc cccatgacca atccaaataa aaagaagaag atggtccaga
     3301 tcccaaagaa aagcaaaggt ttataatctt tgcacatttt gatgatcatc atgatcactt
     3361 tgaatccgtc agagaatgta ttcaattttg attcgctacc ttcaggacga tcgcgataat
     3421 cgattggaat ctcaacgaac ttgaattttt tatctaaagc atgaatcgtc aattctgttt
     3481 cgatttgaaa accagaactc ataataggga agcttttgac aaaaattcga ttgaatcctc
     3541 gatagccggt catgacatcg cgaatttttg ttttatataa gtaagtaatt gtatttttaa
     3601 ccagattatt cccaaaatca tggaaaggac gcttattttc atcaaaatag gttcca
//
LOCUS       DACEXQ010000232         3651 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.232, whole genome shotgun sequence.
ACCESSION   DACEXQ010000232 DACEXQ010000000
VERSION     DACEXQ010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3651
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.232"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            407..1447
                     /locus_tag="IXH04_002478"
     CDS             407..1447
                     /locus_tag="IXH04_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAQ1853042.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
     gene            2271..2603
                     /locus_tag="IXH04_002479"
     CDS             2271..2603
                     /locus_tag="IXH04_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAQ1853043.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            3222..3563
                     /locus_tag="IXH04_002480"
     CDS             3222..3563
                     /locus_tag="IXH04_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAQ1853044.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1348 a          482 c          674 g         1147 t
ORIGIN      
        1 aatctattgt tttctagcct aaagccttgt cttataagaa taatttaata tatactactg
       61 aaataaggta gtatatatta ctagtaatgg tagtatgttt tatatgcata aggtagttct
      121 taatttattt tttgttttga cactggtagt acttaattat ctttaatttt aaaactaaat
      181 tttattcata cgtttttctg gtagtattta attataaata atatttattt aaagtagttc
      241 ttaatgactt taaaggtagt acataattca ttttatttct gattttaggt agtatttaat
      301 taaaaaacag ttttaatgcg tttgcatttt ttatttatct ttgctatttt cgaaaagagt
      361 aggtaatttt acataaataa aacaacgtga aagtagagga tctacaatga ctgattttaa
      421 attttttaag gcggatagag tttacaacga attattttat caatttccaa aagtctttat
      481 tgtttctgac gaatacaaaa aaatgaaaga ttcaactaag attgcctata tgcttttaaa
      541 agcaagatta gagatcgcaa tcagcaaacg gcaaatcgat gaagaaggta atgtttattt
      601 tacttatacg acaaatgaac tatgtagagt attaaactgc caaaaacaaa aagcgatagc
      661 aatcaaaaaa gagttggaat cctttggttt attattacaa aagcagatgg gatttaacaa
      721 acagttaggg aaaaataatc ctaatagact atatctagca gaattaaaag tctcagaaaa
      781 tgatatctac ttactcgaaa aatttgatag agagaatagg gaaaacgttg ataaatcaga
      841 gggtatgaaa atcataccca ccctcgacga aaaatcagac gctgaatccc ttggggctca
      901 agagggtatg aaaatcatac cgtgccaaaa cgttgataaa tcagagggta tgaaaatcat
      961 accagaactt aataataata tattagacac taatagacac aatatagaca ctgaaaaaga
     1021 ccgcctacaa gatcaattgt tgttagacaa ttttgagaca attatgacaa acgacagcat
     1081 tgctacgttt gtccctgaac gagtattaaa tttgataaaa acattttctt caagttacag
     1141 tgaagctcaa aaaaccgtcc agactattca taatgcaaag aaaaaagctg aaatagaaag
     1201 tggtatttcg atagtttttg aagaactcga tagttattat gtcaatgcag aacaagaatt
     1261 atacacgaca ctgttaaaag cctatcaaaa attaaaaacc gaaaaagtcg aaaatatcca
     1321 gaacctgatt tttgtctatg taaaaaattg gtttatcgaa aaaccaatag ctgctaaagt
     1381 atcaagtgaa aaacgtttga attatgaaag ctccccaagc actattacga aagactggtt
     1441 agagtgaaac tttactgaca aagtgattgt tgcttcatgg agtaacatta aaattaaaaa
     1501 ttttttatta ttctaattat attacgaata ttacaaaata atagctattt taatagaatt
     1561 tataagttaa aatattatga taaaaatcac aaacgtagcc ttttacgtca taagtgttaa
     1621 aagaaataat taatttcaaa tatagaaaag ggaacgtatg tttgcatact cctttttttt
     1681 gagttataat aatacgcgaa gagagataat ttcattattt aaacaagcta aaaatcttga
     1741 tattacttct tttcaggcaa tacgaatatt attcgcttac attttttgta taaatttaat
     1801 tgtctgctat gcttgtgatg aattgatttt cgttctgcac ttttggatga taaatttgga
     1861 caattgaaaa tacaaaaaaa agaaaggcgg tatgaactcg gaaggctggt aaccttcgtc
     1921 agcgttcata cacggcatta agatttggcc cttaatgcgt taacctttca gtgttacggt
     1981 attacccgca atggatttag tataacagat attcaaaagt gataccactt tttttggctg
     2041 tgttaaggtt aaaaatacgt gatattacga tgaatctatg cctaagcaca atgttataaa
     2101 aatgtccgtg tggctctttg ccgtatgacg aacgctgaaa ttagagggaa cgcatgtttg
     2161 gatgcgttcc ctcttttttg tttgtcatga acgaaaatca attctctatt tttgaaaaat
     2221 aacaagaaaa acttgttaaa aatatttaaa gaaattgggt ggtttatttt atgtcatata
     2281 caggaatttt gagtctcaaa gatatttgtc actacggtaa acggtgtacc gctacagaga
     2341 aaatcacgaa gaaactatca actggccaaa gtaaaaccgt tgtgcagtgt aagaaatata
     2401 taatccagaa agacaaagtc tcagaggaaa tgatttatta cattgggaaa caaaagcaaa
     2461 taattttaaa agatcctatc cccttaaaag agctatatcc aacaatcaaa catgtttatg
     2521 accaaaacgg tgtactgatt ggtcgaagaa aaaatggagt cttgcgttgt accgcaaaag
     2581 ggatggggcg tttgatcggc tgaattgatc attctcataa cttgtatttt atcgtttttc
     2641 gcttgcttga aaatattgtc gagacttcta aaggaactta gagtcactag aaaaataagg
     2701 agtagacttg atctatcaac aattcccttc ggggagtgac ggggtaggtt ttaacgattg
     2761 gtaacgtaaa aaattggtca atcgagtaaa tgcgcttgtt tggatagtgg tgaaatgaat
     2821 ttgtgtaaat aataagcgca gagtaaacaa gagggaattt agaagttaga caagttagaa
     2881 tagcgtctag attaatcatg agcagctact aagggatcta gactttgacg caaactcatg
     2941 gaataaggag taggtcttct cgtaatgggg aggacgcaca agaaattgtg tcgtgtgctg
     3001 cagtacatgt agatacttgc gaggacgttg attattggat caatcgagca tttaccgctg
     3061 tctactaggg tttctcgttt cggacgccat aaacgagtcg aaatttttta tgtattttta
     3121 agtaaacgaa aaacgataaa atatgaacaa gtcctgaata aagtgaaaaa attgatataa
     3181 aacatagaga aaatgaaatt tacgtaatgg aggaatgaca aatgaaaaat tatgatccga
     3241 atatacgatt gggcacacat actattaagg tttctttcca acgttgggat tacaaaggat
     3301 ttgtgacgtt tagaagaggc ggaaattgta aaggattaga tgttttagca ttagacgagg
     3361 atgatttata cgatcaaaca ttgactgata atccaattgg attcggcttg ctacctgaag
     3421 atgatgaagg taatgaatgg tttaaaatga cgcttatgaa tgataatgga gacgaattat
     3481 ctgttgaaga tacttggtca tatttgagtg attatattgt cagctttgaa atcattgaat
     3541 ttgtagcgga taaagaagaa taaaaactgg aggaataaat tttacagata aaggcttaga
     3601 aggagtcacg atgattaata aaaaggtagt attaccttag gcattgagta a
//
LOCUS       DACEXQ010000233         3614 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.233, whole genome shotgun sequence.
ACCESSION   DACEXQ010000233 DACEXQ010000000
VERSION     DACEXQ010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.233"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            454..660
                     /locus_tag="IXH04_002481"
     CDS             454..660
                     /locus_tag="IXH04_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAQ1853045.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(862..1863)
                     /locus_tag="IXH04_002482"
     CDS             complement(862..1863)
                     /locus_tag="IXH04_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1853046.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1868..>3614)
                     /locus_tag="IXH04_002483"
     CDS             complement(1868..>3614)
                     /locus_tag="IXH04_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAQ1853047.1"
                     /translation="SSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSN
                     DESSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELL
                     VTELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPN
                     TKQLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAG
                     YNLLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMM
                     SVLAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDL
                     FMGLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGG
                     YKDLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEI
                     QLYGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPT
                     ENADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFL
                     SVSTIITWIWRRKRG"
BASE COUNT          962 a          720 c          557 g         1375 t
ORIGIN      
        1 tagtatacag tagtaaaaaa tttatctttt ctttttttta ttatttttgt ttcttatttt
       61 ctaaaaaatt aaaaaaaatt ttggtgaatt aagtatttcc ttgataacac tggcttttgg
      121 cctcatttta ggttatcatt tgtaatttta tttcacagtt gatatgattg ttatgtaaaa
      181 taaaaaagtt taggatacat cgaatttttg gggaaattct tttgggggta tcctaaatct
      241 tttctattat attcccatat tttctattat tggtctagac gtatgatggt aaactttgta
      301 cctatctgat tgctgaatca ttaattgctt atcattagcc atctttgttt caagttcgct
      361 agatgatcat tttattcaca tcctaacaaa cttgtcacat ttatagattc ttaatctatt
      421 tatcatacaa ttataaaaaa ggaggtgctg cttatgaaag ctatccatat ttctttgctt
      481 tttttcgcta gtgcattgat tttatttgct attcatcttt gttcccagcc ttatatcaat
      541 aacaacggaa tattagtgga accagcattc ttttgcttac cactcggctt tttgtccctt
      601 cttgcctccg caggcttcgg atttgttgct ttttttaaac gatcaaaaca acaaatctga
      661 cttataaaat ttacgtaaaa gagatcgtga caataatcgt gtgatattcc caaatttcga
      721 ataaaaggtg ccccttgcca tctctaactc ctcagtcaca agttacaata ttcataaacc
      781 atgaaaagct tgtttattga aattgctcct tgtgacttgg agctaagtga cagattacaa
      841 cctcttttgc atcataagac tttactcctt gaaaaacatt actaactttt gatatgtttc
      901 ttgcgcttgt ttattgctga aatcaaactg atattcatgg ttcaaaacag catttttctc
      961 cgcataatct acttttgtga ctttcacccc cgctttctca aatacagaag ttacttcttc
     1021 catttgtttc gtaaacgtat ttgtatttcc atccgttaag aatgtgtttt gcgtccaacg
     1081 atccgcatgt ctgaccaagg ctaaactttt gaccttctct gattgttcaa agtccttatt
     1141 atcaaagtaa gcccatccac atttttcata aagaaagcta gagattttat cacttacttg
     1201 agtaagtgca gaaggatcca acagaccaga caaagaaata aacttcgtaa tctgcttgtt
     1261 ctccaaagtc ggatcaagat gatcagcttt tttaatttct tcattttgaa gtgctaacaa
     1321 atattctcca ctaatttgtg cacctgccga atttccacca atcgaaatat tcgaccaatc
     1381 tgcttgatct tttatctctt ttttagctgc ttgaatggct tcgttcaatt ggataactgg
     1441 tgtaggatgt tttgcttccg gtgatcgttc ataatctata tttacgatga tttgctgcgt
     1501 gtctgaacat agatattcca aataatcacg acaatcattt ttatcccctc ctacatatgc
     1561 gcctccatgc atccagaaaa gaatcggtaa ttttttcttt tccactttcg gtgtgtagat
     1621 atccatatta ttatttttgt actttgaagg ataggatatt tcttttttta tatcgatttg
     1681 ttcgattttt tgctggaaat catctggtcg ggcatatact tgttcttttt cacttgtgtc
     1741 aaattgtttt ctaacaagca agctaatcgg ctttggtgtg atttgattaa tcaaaaatac
     1801 agtgactaaa ataactacga tgataactgg tactgcaatc actattcgct tgaaccattt
     1861 cattcattta ccctctcttt cttcgccaaa tccatgtgat aatcgtgcta acagataaaa
     1921 atccacctga ataaatgaga agcgaatgtt tcttttctcc agttttcggc aacattcttt
     1981 tttttgcatc tgtctttttt tgtactttca ctgtcatctg aatcggttgc gtatcatcac
     2041 ttgattgatc ctctcgaggt tcattctttc ccttattgtc tgcattctct gtcggatctt
     2101 gcttttttgg tggagctact gggatttttt tgtaatagaa aataatagtt ggtatcgtct
     2161 cattaaaaat cccttcagtc ggtccttcaa catttgctaa ttcgtatcct tcgaatgtct
     2221 tttgctctgt catataattt tctccataag ctccgtaaag ctggatttcg tcctggtctt
     2281 tgagtagact tttattgttt tcatccacat actttacgtt tatctgatca tggaacacta
     2341 ctttcaaaaa gtccgttaca tgtttcgtgt aggcagcatg gtcaacttcc tgtgcctttc
     2401 catgccctgc tccttctact aataaaagat ctttataccc tcctgataac tcatataatt
     2461 tttgcgcgtt agacacaggt actacatcat ctgctgttcc atggataatc aatttaggca
     2521 atgaatctga cattccaatc gtaggtagag cagcttttgt atccaaatgc atctcctgat
     2581 caaaatagta ttgtttcaaa aggcccataa ataaatcttc atgctcttga tccccaatga
     2641 tgtctaactg attttttatg gcatccggta gtgcgggggc tactgtctgg ctgtacgtaa
     2701 aacgtagttg ttcatcaatt gatgcaaatc cgcaattttc aataattcct ttgacattga
     2761 caggaatatc tttcgctaat acagacatca tcgtagctgc tcccatcgaa ccaccataaa
     2821 gtaaaatctt ttgagatggc cgctctcgaa cttcttgatt tatccagtaa aggacgtcat
     2881 ctgactcata ttgtccatag gtcacataat cgcctccgct attaccagtc gctctggaat
     2941 caacaaataa taaattatac ccggccttat aaaaatcatt gtattcttct gtaacgatcc
     3001 cgttattcca attccctcga aatccgccat ggatcatgac tgttttatct gaaccttgat
     3061 cgacataata tgcatataca tccatcattt tgccatcttg tttgtttagt acagtttgat
     3121 gaagttgttt tgtatttgga tagaagcctg tgttttctgt aggcgttgtc ccatcatctg
     3181 tacctccaag catcgacatc aacacactgt tttttgcaaa agcgatatcg tacaatagat
     3241 aagtagcaat cagatgtttg ataatctctt tatctacacc ataatcatta acagcaagct
     3301 cagtgactaa taactctatt aattttgact tgatgtcttc tgttggtcca ttaagtattt
     3361 ctgtcatctg tttaccgaag taattcaaat attctttacc attgaaattt tcacctttac
     3421 cgatatcatc attttctgtc ccattctctt tgtcacttac tttagcagaa gaggattcgt
     3481 cgtttgacat ttttgtagct gttgcttctt ctgtttgttg gttatctgtg gtagcggtcg
     3541 tttcttgatc tgtgctagta acagatgtct caaccgttgt ttcagtattt gtttctgttt
     3601 gtgaagtact cgat
//
LOCUS       DACEXQ010000234         3590 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.234, whole genome shotgun sequence.
ACCESSION   DACEXQ010000234 DACEXQ010000000
VERSION     DACEXQ010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.234"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..447)
                     /locus_tag="IXH04_002484"
     CDS             complement(<1..447)
                     /locus_tag="IXH04_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ1853048.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
     gene            complement(754..1851)
                     /locus_tag="IXH04_002485"
     CDS             complement(754..1851)
                     /locus_tag="IXH04_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAQ1853049.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            complement(1959..>3590)
                     /locus_tag="IXH04_002486"
     CDS             complement(1959..>3590)
                     /locus_tag="IXH04_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HAQ1853050.1"
                     /translation="SQRGAIWSNKKISLLSDFTFKSYLYLGNQRDRAGDGMTFTLTGD
                     PRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYKNSGSSDRMDREITADN
                     RGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLIIKWDAEQRSLTYDLEGV
                     GHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLPSNVTSTADVKVNDSDY
                     GAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTIPEHILINGNQIDAKDI
                     LITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHFEYLENGNLIEQSNEIN
                     LKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETPIDIEGYVFDSDSANTE
                     GIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSEFGQFSGGLKIIDERAF
                     GSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTIFESDQKGESDLSSLLD
                     STAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
BASE COUNT         1040 a          702 c          556 g         1292 t
ORIGIN      
        1 caactttctc ttttcaaaaa aaatttgata tttttttaga tttcttttta gtctttgtaa
       61 tactttataa gccacagaat aactcacgtg atgctgttta gcaaaatttt ctactgagat
      121 atatttttcc tgaaagaaag ccaataccat tttcatagat aaagaatccg ttatataacg
      181 agataacaga gctttgctag aacattttcc attaatttcc agacatatat ccaattcact
      241 gacggttact cgcaaatttt tctctagatc gaacttttgt aaatcatgtt tcagcttatc
      301 gatcgttttt ttcaagagaa atgtcgatag cgacaactct tcttgaacat tcgatattga
      361 ctctgataaa tttgagcttg cttctaaata tttaagtaat gaataacttc tgtgatcttc
      421 tttgcttaaa aaaaactcat aattcattag tcatcagctc tttctacgta tattactttc
      481 catgcattca ccaactagct ggggataaaa ctgatttcct ggatccgagt cactgttgtc
      541 agcgattctt gtttgtataa tatacatcgt cctgtctttt ttttaacatt ttggagagcg
      601 ttttatttgg cctttctcgc gatcaaacct gttattagaa ttatatcatt tactagtatg
      661 catttttatt aataaaatgg tatattttat attgttataa aaagagccca accatcctac
      721 aataataaaa atttactatt tgttatctta accttacttt tctttctctt ctttttttct
      781 caaaacaaac aaaagtatgc ataataaaat aaacaataag aaaagtgaag ctactaagat
      841 ataaatccaa ttattgtctt ttttaatcgt aacatccgtc gtgttcaatt ccttggctac
      901 ttttccgtct attgtaaaat ctttttcaaa tacccattga ttggtatagt tgatcgtagt
      961 atttcctttt tttctcgaaa attttccatt ttcattttca tttcctaaaa cagtcattgt
     1021 aaggtgatat tcacctgggg ttagcggttg accatttaaa gcagttggaa aagaaaaatt
     1081 tgtattcggc gcaatctgca atccttcttt ttcctcttga tataaaactt cagaatgtcc
     1141 tttttttgta atttttgttt caataactac ttgattgata taggtttttt gatcattttg
     1201 aacgtttgct aagatgacat ttcttgcatt gatttgatct ggtttaactt catgaagagt
     1261 aaggttcgga gcaacttcgt tcagtgtctg ttgcatcaac aaagcaataa tataggtgta
     1321 ttcattctca atcgataagc tttgatcctt agtttctgtt tggtcctttt ccgtctggat
     1381 ttcttggaat gtgatcccac cagccaatac accatcaaat cgctcattcg gcatcctgac
     1441 atgaaaagca acggtttgga cacttttagg ttttagcgtc actatctgtg gatagctcac
     1501 ataatctacc agatcaaaga tcaggctcat gtctttttca atctcgtttt ttccgtattc
     1561 aaccatcaca tttgaattgg tggttgcgct attcacagaa atatcgattt tcacttcttt
     1621 ttctgtatca tttctcaatt ccgcttttac ttctacttcc tgatccggat caagttgaag
     1681 atcgaagtaa gtttttctct tgtcgatttg attctctgaa gttatcggcg tcactgaaaa
     1741 attaaatgca ctagctgaca ctgatatatt gttaaaaagt gcccctaaaa taactaggag
     1801 tgttataaat aattgttgtt ttggtctcaa gaaaatcgct cctttaatca tatgtaagag
     1861 aagactataa caaaatcttg tctctgtggg acacaagagc ctatggtacc tgtgcccttc
     1921 ttgaaactca ataattttgt tatattcttc tcttcctgtt aatttcctgg gacatctgca
     1981 agtatccaag atagtttccc ctcaaaggtt ccgattcttt ggttttctac aggaatatct
     2041 attctgattc cacgatgagc tgtagaatct aaaagagagg acaaatcgct ttcacccttt
     2101 tgatcacttt cgaaaatcgt aacagcggat gtccctataa ccgttgtatt ttcagaggta
     2161 acaaaggaaa gagcttctgg aagctcaaaa tctccatttg taagaacttt ctcttgtttc
     2221 aattgtaatt tccaacttcc aaaagcccgt tcatctataa ttttcaaccc acctgaaaac
     2281 tgtccgaatt cacttaatgg tctactggat attttatgtt ttccaaaatt gacatattga
     2341 ggagcagttg agagataaag tttgcctttt ctgtaataaa aatagatatc ctctgtattt
     2401 tcggagaaga tgccttctgt attcgctgaa tccgagtcaa aaacataccc ttcaatatca
     2461 ataggagttt ctttatactt ctctcctatt ttcccagtga ttgttttcat ttcagcaata
     2521 tcaatctgac tgtctttatc tttataataa acatggatat ctatctcctt cagctttgcg
     2581 atctttaggt ttatttcatt cgattgctca atcaaattcc cgttttcaag gtattcaaaa
     2641 tgcatagtga gtactttttc aggaattact tcctgcaaag ttgtctccaa ttcgattatt
     2701 aacgtatctc cagcaataaa ataatctgtc agatttagcg tgactcgatt tccggttatc
     2761 aagatatctt tggcatcaat ttgatttcca ttgatcaaga tatgctcggg aatagttaat
     2821 tcttgtggga ggtcgatact agtctgcatt ttcttatatt caagaaaatc cccctcgacc
     2881 ttcaatgtat ttctgatagt tatcttatcg tttcctccag cttcagcgat ggccccataa
     2941 tcactatcgt tgacttttac atcagctgtt gatgtgacat tcgatggaag tcgcgtgaca
     3001 gcgacagcat tttcttgaaa aaaagtacct gttgaagaag taaaccccca atttacttct
     3061 gtagaaccaa actgcctaat gagatcaggt ataatccgag aagagtgacc gacaccttcc
     3121 aaatcataag taagacttct ttgctccgcg tcccatttaa ttattaacaa gcgccatgtg
     3181 ccattagaaa gaaatgtagg agctaccgtc acgccagtat gctctccaga ataattatta
     3241 ttatttggtt taggcgtgac aaaagcgaca tgtccatgcc ctctattatc agcagttatc
     3301 tctcgatcca ttcgatcaga agaaccacta tttttatacg tatcaaactc aatcgagagc
     3361 gcattcgcaa tgtaaggttg gccagaacga gtagaatatg agccaattcc catacctggc
     3421 gatccaataa cttgactggg attactagcc attctaggat cgccagtcaa tgtaaaggtc
     3481 atgccatctc ccgctctatc tcgttgattt cctaagtata aataagattt aaatgtaaaa
     3541 tccgagagta aactaatctt cttatttgac caaatagctc ctctttgaga
//
LOCUS       DACEXQ010000235         3551 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.235, whole genome shotgun sequence.
ACCESSION   DACEXQ010000235 DACEXQ010000000
VERSION     DACEXQ010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.235"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..2178
                     /locus_tag="IXH04_002487"
     CDS             <1..2178
                     /locus_tag="IXH04_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853051.1"
                     /translation="INQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYESQQQQLLA
                     QLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKRYQQTNER
                     LEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRLQIETRET
                     NSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQIAKEQQEM
                     IEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLKVILLLIG
                     AGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKRTGEEKQI
                     LQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQEKLRQID
                     QKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEINQLKKKQK
                     VWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPEELTKEKL
                     SVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQKAKLQEL
                     LEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLKNDTLTLT
                     DGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQRKYQLAE
                     LLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            2162..3106
                     /locus_tag="IXH04_002488"
     CDS             2162..3106
                     /locus_tag="IXH04_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1853052.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(3175..>3551)
                     /locus_tag="IXH04_002489"
     CDS             complement(3175..>3551)
                     /locus_tag="IXH04_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1853053.1"
                     /translation="GEISDVNTTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDI
                     ATETKLSDNAFTTKVIGEELKDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKS
                     SDTKSTDSTKESSTEETTDSSK"
BASE COUNT         1277 a          584 c          706 g          984 t
ORIGIN      
        1 atcaatcaaa aattaaatga atggaaagag ctgcaagaaa aaattcatca aaaacaagaa
       61 caggaagctt attttggcaa gttgatccaa caagaacagg aatacgagtc gcaacagcag
      121 cagttattgg cacaattaaa agaggcgaac aaacaatttt cattagccag acagcaagaa
      181 cttaattttt ctttatatga agaatggcaa tctctcaaaa aaaacagcac tacaggtctt
      241 cctgatgagc aacttctttt agatctggag tctttctcca aacggtatca gcaaacaaat
      301 gagcgtttgg aagaattaga agcaacactg gaaaagcata gcgggatgga tcagcagtca
      361 gccagatatt atttttattt agagcaggag caggaactaa aagatctcca gcaacttcaa
      421 tttacagcgg aacaattaaa cgaagaatgg caaagattac aaattgagac tagagaaaca
      481 aattcagaat tgaagatttt ggaacaaagg tggcattggc agaaaaatcc gttaccggaa
      541 gaatttttat atgaagaaga atggaagcat cttctggctg aagaaagaag gattgctgat
      601 ctagaaagcc agatttctgt gcgtctccaa atagctaaag aacagcaaga gatgattgaa
      661 gcagaagtga atgacttgga agagacctat ccagcattat tacaaaatca gtcagagata
      721 gcaccagtct cttcatctaa accgtatatg ctgatggctg gcggagcaat aatcattgca
      781 ggtatatttg taccaatgcc cttgaaagtg atccttttac taattggtgc aggcatgatt
      841 ggctatttct tctacaaaag gcaaccaaaa attaaaacta aaaatgattt ctctgagata
      901 aaaggaatgt ggcaagaaaa actaggacaa cttgatctag ccttagctca attagctgaa
      961 gcagaagaaa aacaaaagag aactggagaa gaaaaacaga ttcttcagca agaagtatat
     1021 cgttttgcgg atcatcggca cttaggaaag atggatacgc tcaaactcat gaaagaatat
     1081 ggacaggaag tcatagatta tcagaaatta tatggcgtat ttgagcggaa acataaaaga
     1141 gaacaggaaa tacaagaaaa actacgacaa atcgaccaaa aatttgagtt tgttaaagaa
     1201 tggttaccga tacaagataa gacaatttca gaaaaaatgg tattgctttc ccgttttata
     1261 caagagatgg aacagatgaa atttgccaga agctatcagc aaaatacttt attgaagcaa
     1321 gaaatcaatc aattgaaaaa gaaacaaaaa gtatggatac aagaatacag cgaacaattg
     1381 actcaagcag gcttatctta tccttcggaa gtacctgtct ttttacaaaa agccaaacaa
     1441 gccgaacagc agatgaaaag atacaatgaa ttatcggaaa tgcttactcc actatttcct
     1501 gaagagctga caaaagagaa attatctgta agactgcgcc aactgaaaga aaagcaatcc
     1561 caactccagc aagaagaaaa agaagcaagt gaaaatcggc aacgactcca acttcaagta
     1621 gaagagttgc aaagagacgg cacattggac gaactttatc aacaagctag ccggcaaaaa
     1681 gcaaaattac aggaattact ggaggaatgg ggagcacttg aaatcgccgg gacattctta
     1741 ggtgatcttt caacagagat gagtgaaaga caattacccc agttgttaag agcagcaggt
     1801 gattatttag gtattttaac aaaaggaaat tatcagaaga tattactgaa aaatgataca
     1861 ctgactttaa cagacggata tacagaattt cccatctatg agttatctac aggtacgaaa
     1921 gatcagctga ttatggcgat gagatttgct tacctctcat tagaagctaa ccgttcgata
     1981 tgcccgatca tcattgatga tggctggctt cattatgatc atcagcgaaa gtatcaatta
     2041 gctgaacttt tagctaaatt tggagaaaag tatcaaataa tttgtttttc ttctgataaa
     2101 gaaatggtaa gctattacca tgagatgaac caaatcgtaa aacaattaga aggagcaaat
     2161 aatgaaaaaa attcgtgatt tagtaatcga tgagcagttt gaacaatttg tcctgatcaa
     2221 aagtgcagat gtaagaatcg ctaaaaacgg aaaaaaattt attgctttta ctttccaaga
     2281 tacttcaggt actatcgatg gtaaatactg ggatgcttcg gaggaggaaa tcaataaatt
     2341 tacagccggc acagtcgttt ttctaaacgg taaaagagag gtttatcaag gaaaccctca
     2401 agtaaagatt cttcatatgc gcttaacaag ggtagatgag ccaggagagc ctagtttata
     2461 catggaaaga gcccctttga agaaagaaga aatggaagaa gaaatcaatc aattgttgtt
     2521 tgagatcacc aatgcgcatt ggaatcgtat tgttcgctat ctacttaata aatatcaaaa
     2581 acaatttttt gagtttccgg cagcaaaaag aaaccatcat gcatttgcca gtggattagc
     2641 ttatcatact ttgacaatgc ttcacttagg gaaatcaatc gccgatgaat atcctgagct
     2701 gaatcgagca ttgttgtatg ctggtatcat tttgcatgat ttaggaaagg tgattgaact
     2761 ttccggtcct atgtcgacag aatatacgct tgcaggaaat ttgatcggtc atctggtatt
     2821 gattgacgaa gaaataacta aagcctgtct tgcgttgaaa atcagcgaag ctgacgaaga
     2881 tgttctcgta ttacgtcata tggttttagc acaccatggc ttattagaat atggctcacc
     2941 tgtacgtcca agaataatgg aagcagaaat attacatcaa atcgacaata tcgatgcttc
     3001 gatgcagatg atgttgactt cgattcgtca aacagaacca ggaaaataca ccgatcggat
     3061 tttcggtatg gacaaccgaa gtttctatgt accaaaagat ttatagcaaa aatttatttt
     3121 tttcattcaa aacaaaaaaa gcgagacata agtcttcgct ttttttgttt tcttttattt
     3181 tgatgaatca gttgtttctt cagttgaaga ttcttttgtt gaatcagttg attttgtgtc
     3241 agaactcttt gttgaagctg tagtttcact tgagtctttc gtacttgatg aagttgttgt
     3301 gaatgcactc aacacatttt caaaggcatc atctttgatt tttacatttg catcttttaa
     3361 ttcttcaccg attactttag ttgtgaatgc attgtcactt agtttagttt cagtagcgat
     3421 atcttttagt tgatctttgt atttatccat gtcattgcct ttattttggt ttttcaccat
     3481 tttgacaacg tagtattctg tcgcataaga agtaggattt gttgtagtat taacatcgga
     3541 gatctcaccg t
//
LOCUS       DACEXQ010000236         3527 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.236, whole genome shotgun sequence.
ACCESSION   DACEXQ010000236 DACEXQ010000000
VERSION     DACEXQ010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.236"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..250
                     /locus_tag="IXH04_002490"
     CDS             <1..250
                     /locus_tag="IXH04_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853054.1"
                     /translation="RVDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETS
                     KPNVLVRWNLVAHRLGYEDIRLKEDTAYDSTVLDQKRF"
     gene            274..2511
                     /locus_tag="IXH04_002491"
     CDS             274..2511
                     /locus_tag="IXH04_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853055.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVIFSLDGTFMSEAGYYKHCYVEYRKGNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGGYDYSGNPNLMANINADSFS
                     QGSGALSVVDDGDEVVITLDPNHKLEVFKPKSQPALLTGKTYTVSVEIMLEGDFTGDP
                     SKIGLRYFKMPNWVPALTTPNALTTAKGVWQKLTVTAKMTTAIDNAESWYVTLYNKDA
                     NNSLSGKLRLRHAKLEEGSTATPYQPNLLDAPYYLSKVALGENLVTNAKFPIITPNNP
                     ISGFDISEELIIGETYTVSLKGTKPANKEFHLYYGDANYQQSQSQYQATLLPVEGLAN
                     VWSATFTARNTSETTNLLRVALWQKPNSATYGTVQIDWLKIEKGNTRTPNISEYKYRG
                     TGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANDYTDGKVSEINSQLTASIN
                     EVDKKVTANTKSITTINSTLTEMQKPVKYEAWFSGGTELRPANANSRFRVSEERLTIG
                     TKLNVAMNESPLEWNADRWEATFTRECSILVDVQTMVEFADQWGTYVYINMWKDTNQT
                     QIWDTGYGMGIASGANFWFRTELSVSMYCMNAKVGDKFSIGLGMASGKSLTSARIHKL
                     HIMEL"
     gene            2593..2835
                     /locus_tag="IXH04_002492"
     CDS             2593..2835
                     /locus_tag="IXH04_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAQ1853056.1"
                     /translation="MKDEAIQDVVERLVRIETKLDNYESLREKAESAKDRADQAYSIA
                     LNNAEDIKEMKANNKWSWGYMIGLGITIIGYFLTKL"
     gene            2851..3042
                     /locus_tag="IXH04_002493"
                     /pseudo
     CDS             2851..3042
                     /locus_tag="IXH04_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
     gene            3058..>3527
                     /locus_tag="IXH04_002494"
     CDS             3058..>3527
                     /locus_tag="IXH04_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1853057.1"
                     /translation="MKKKIILSLSLLTALFLLPINGFAYTINNEFNLGANEGSSQVAN
                     NQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSY
                     ANANSPVQIELQHTHNKATFEKNYKAYVELARDSAKKYGIPLTLDTPYNQPGIKS"
BASE COUNT         1220 a          570 c          750 g          987 t
ORIGIN      
        1 tcgtgttgat attgacccca tgtttgctga gacagtaaat ctaagtcggt atatgacaca
       61 tgtgacacct acagaactag ttttgtgtgc tgttactcat gaagatattg accatttcat
      121 cattgaaact agtaagccaa acgtattagt tagatggaat ttagtggcac accgtctagg
      181 gtatgaagat attagattaa aagaggatac agcatatgat agcacagtgc ttgaccaaaa
      241 acgtttttaa aacgaagaca aggaggtata taaatggcta gcagtttata taatttggct
      301 ttagatttca gcaaagaatt aaactacacc aaagctatta tggctcgtca aggtgataaa
      361 gggattacgg tgactgttaa accattttta aatggcttgc agatggatac gagtggcgga
      421 acatttactt taaaaggaac aacaccatct aaccgttatg tagatagtgt tgcaactagc
      481 gtaactagtg aagaagtcat attttctctt gatggcacat ttatgagtga agcaggatat
      541 tataaacact gctacgtaga atatagaaaa ggtaatcaaa ttttaacaac gcaagatatc
      601 atttttttct cactaggagt gtctgacatt tcgcaaggtc aagccgatga atacgtttcg
      661 caattggaag agttgattcg aaagtataac gaaacttttg atgcttttat ggctgaaatt
      721 aaaggtagag tggatagctt aaatcaacag attactgatt taactggtca agctaaaacg
      781 ctacaagaca agttagatgc tctgaaagaa gaaatttcta aattaggtaa cttgcaagtc
      841 atgtacagta acagcatcga cttcgggggc tatgattata gcgggaatcc gaatttaatg
      901 gctaatataa acgctgatag cttctcgcaa ggtagtggtg ccttatctgt tgtagatgat
      961 ggtgacgagg tagtgattac gcttgatcca aaccataaat tagaggtatt caaaccgaaa
     1021 agtcaaccag ccctattaac aggcaaaaca tataccgtga gtgtagaaat tatgttagaa
     1081 ggcgatttca ctggagaccc tagcaagata ggcctaagat attttaaaat gcctaattgg
     1141 gtcccagcac taactacgcc taatgcatta actactgcta agggtgtatg gcaaaaacta
     1201 actgttaccg ctaaaatgac tactgcgatt gataacgctg aaagttggta tgtaacacta
     1261 tataataaag acgctaataa cagcctatcc ggcaaactac gtttgagaca cgctaaactc
     1321 gaagaaggct caacagccac accatatcaa ccaaatttac tcgatgcgcc gtattatttg
     1381 agtaaggtgg ctttgggtga gaatttggta acaaatgcta agttcccaat tataacacca
     1441 aacaatccta tttctggctt tgatatttca gaagaattaa taataggtga aacttatacg
     1501 gtatctttaa aagggactaa gccagctaat aaagaattcc acttatacta cggagatgca
     1561 aattaccagc agtcgcagtc gcagtatcag gcaactttac ttcctgtgga aggattggca
     1621 aatgtgtgga gtgccacttt tacagccaga aatactagtg aaacgacaaa tttacttaga
     1681 gtagcgttat ggcaaaaacc taattctgca acatatggta ctgttcaaat cgactggctc
     1741 aagatcgaaa aaggcaacac acgaactccg aatattagtg agtataaata tcgtggtact
     1801 ggtatgcgtg attcaaacaa tccaaaagat tatgtttggg atctagcacc agaatatgtc
     1861 gaagataatt tggccacaga tattaaaatt tctgaaatca caggcaaagc aaacgattat
     1921 accgatggga aagtatcgga gattaattcg cagttaactg cttcaattaa tgaagttgat
     1981 aaaaaagtaa ctgccaatac gaaatcaata accacaatca acagcacgct gactgaaatg
     2041 caaaaaccag tgaaatatga ggcgtggttt agcggtggca ctgaattgag accagcaaac
     2101 gcaaattcta gatttagagt aagtgaagaa aggttaacca ttggtacaaa actaaatgtt
     2161 gctatgaatg aaagtccttt agagtggaat gctgatcgtt gggaagcaac atttactaga
     2221 gagtgcagta ttttagttga tgtacaaact atggtagaat ttgctgatca atggggtacg
     2281 tatgtttata tcaacatgtg gaaggatact aatcaaactc aaatatggga tacaggttac
     2341 ggtatgggta ttgcaagtgg cgctaacttt tggtttagaa ctgaattgag tgtatctatg
     2401 tattgtatga atgctaaagt tggcgataaa ttttcaattg gtttaggaat ggcttctggg
     2461 aagtctttga caagcgcgag aatacacaaa ttacacatta tggaattata aaatttccaa
     2521 ttaattaaaa actgctacta gcgtactcaa tcgagtactt tttttatttt gcaatgaaag
     2581 gaggctagtt ggttgaaaga cgaagcaata caagatgttg tagaacgttt agtgcgtatt
     2641 gaaacgaaac tggataatta cgaatcatta cgcgaaaaag cggaaagtgc aaaagataga
     2701 gcggatcagg catattctat tgcgcttaat aatgcggaag atatcaaaga aatgaaagcc
     2761 aataataaat ggtcgtgggg ttacatgatc ggtttaggca ttacgatcat tggctatttc
     2821 ttgactaaat tgtaaaggag gtgagaagaa atgattttac cagataagta ttatcaagtc
     2881 attaaatgga cggttttaac agtattacca gctgcatctg ttttagtagc cacgttaggc
     2941 aaagcatatg gatggaatgg aacagatatg acagttctga ctatcaatgc agtagcgacg
     3001 tttttaggtg ttatcactgg tgtgtctgca tataacttaa aaaataggag gaaaaaaatg
     3061 aagaagaaaa tcattttatc attgagccta ttaacggctc tttttttatt gccaattaat
     3121 gggtttgcct atactatcaa caatgaattt aatttgggcg caaatgaagg tagctcacaa
     3181 gtagcaaata atcagtacat tttactgcat gaaacggcta atgaaacagc aacaggacgc
     3241 aatgaagcgc agtatatgca acgttcatgg actagtgctt acactgctta cattgtgggt
     3301 gatggcggaa ttgtttacca agttggacaa cctggttatg tacagtacgg tgctggttcg
     3361 tatgctaatg ccaacagtcc tgtgcagatt gagttacaac acacacataa taaagcaact
     3421 tttgaaaaga actataaagc atacgttgaa ttggcgagag attccgctaa gaaatacggt
     3481 attccgctta cattggacac gccttataac caaccaggaa tcaaatc
//
LOCUS       DACEXQ010000237         3525 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.237, whole genome shotgun sequence.
ACCESSION   DACEXQ010000237 DACEXQ010000000
VERSION     DACEXQ010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.237"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            119..2179
                     /locus_tag="IXH04_002495"
     CDS             119..2179
                     /locus_tag="IXH04_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853058.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2262..2831
                     /locus_tag="IXH04_002496"
     CDS             2262..2831
                     /locus_tag="IXH04_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1853059.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSQQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3006..>3525)
                     /locus_tag="IXH04_002497"
     CDS             complement(3006..>3525)
                     /locus_tag="IXH04_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853060.1"
                     /translation="YFSMWDTFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAV
                     VSFCFSMTVGVMWEFVEFSADKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRT
                     IIESQNADGKTDKTIIQNGYLDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFT
                     FIKNFIPKKIKR"
BASE COUNT         1253 a          639 c          656 g          977 t
ORIGIN      
        1 aatcaaaaaa aatactctcc taatagtcca tcttcttttg attttattta tataatgaaa
       61 tcatcttatg aaaattacta cttcatgttt agacaagagt agggaggact aagaataaat
      121 gtgttcaaaa agacgttcaa aacaactaaa taaacacgct tgtcgtttag ataaggaacg
      181 gtcgtgttct gaaaaacact acgttaagtt tatgatgcgc acgatgctga tcagtttatc
      241 tgtaggtgtc tatatgcatg tacccgttca ggcaacgaca caaaccatca aggtcgtacg
      301 tacctctgtt tctgagacca ctgattcccc tatcactatt actcatactt tcactgatgc
      361 aaatggaaaa cagctacttc ctgcattgac ttcctataat aaagtcagtt atcctccaac
      421 gataaaccac tactggttaa ttcctaacaa aacattatgg acatcggaaa ccggaagcag
      481 tgtgccgctt tctatctttt ccgatatgac tgatcctaca aaaggtgatc agttaaaaca
      541 gatcactgat attttgaatc atgctttgac agatataaag gacggtggaa gcgcacaagt
      601 ttcttatgta tatgaaaaag attattcaca gtttgaaaga aagaaaatcg atgttctggc
      661 agggacaaaa gtttcgatat ctgatctggt agaaaggctg aagaacgcag atggaacaca
      721 aggagatatg tccaaagtaa tttcaaacaa tgggaatatc gtggatacga ctaatacagg
      781 aacatctact atttccttgt cctattttga tccaattgct ttaaaaacaa tgactgcatc
      841 tgctgtgatc catgttcgtg ctgatcaaac agctatcaaa ggaaaagatc ttcacttaag
      901 aacaggagag agttacgccc ctaaagatct tgttgaatat gtaacaaaaa gcgatggaac
      961 ttcaggaaat aaagaagaaa tcaaaatgaa cgggacagta gatactacaa ctccgggaga
     1021 atatcccgtt gtattaactt atatagactc gaaaacatca aatgtggcag aaacaacagc
     1081 tgtagtttat gtagaagcat ctgctgcttc atcgactgtc atcactcatt ttatagatag
     1141 tcaaacaaat aaagaacttc aagcagaaac acatatgagt agtgtaaatc gtattcctga
     1201 tttgcgccta cctgctgaat tttctgatcg agtgccaaat atagatttat cgacttatga
     1261 acaaaatggg aaagtcactt ctatttctga ttggatgcaa gcaaacggat tagacagaaa
     1321 aagtcactgg tcggagatcc ttgaagtact tcactcagaa ctgatgaata ataggaagca
     1381 atcaggaggg gaaattacct ttacttatgt ttatatacca gacaatcgtt cattagaagg
     1441 tcatgatctt attgttcatc caaacgaaaa aataaacaaa gaagagttga tccaatcagc
     1501 aacaaattcc ttaggtaaac ccgtaccgtt aaaggatgta tcggttacaa tcaatgaagt
     1561 accattggag gatggttatt tgaccaaaca aacaggtgat tttacgatta tgtatagtta
     1621 tcatgacccg tattcactcc aagaactcca tgctcagtca ctattacatg ttttaccatt
     1681 aaacaaacag atagatcaaa aagaacaaaa aagacaagaa gaaagcttgt cagaaaaaaa
     1741 acaacaaatg gctcatgcag tttctccaac agggaaagta aaacaaacaa gaagcctaga
     1801 gttagcaaga atatctagaa aaatctcaga atctaataaa acgataaggc aagaagctca
     1861 tttggcccca caagtttctg gattggaaaa tgaaatttta gagcctaaaa taaaacaaca
     1921 atcagaaaaa gaatttgttg ctaaacagtc ggaagatcag aaggaatcag atgaccagaa
     1981 ggaaacgaag aaaaaacgct tgtcaactat tgattccacc gatgagcatc tgcagccgca
     2041 agtaccactt cctagtccgg gaacacccgc acctggtgga ggtattcctt cttctgcttt
     2101 aggagatttc atgcgaggta tggcaggaac atgggtatac gctaatcgaa atgaatttga
     2161 cgcagatgac tggttctgat ttatatctct atctgttata gtcaaataag tagctgagtc
     2221 atatgttatt tggcataatg gagacgagga gggatagaaa aatgttagat aaaacgttac
     2281 cttacgcaga aatctggatg acaagaccac ttcaacaaaa tgttccaatc acgcctttag
     2341 cggaaggttt tgaatttcga ttttatcaaa aagaagatga atctgcttgg gcggagattg
     2401 agacttccgt tggggaattt caaacgacag atgaagcact tgagtatttt caacaaaact
     2461 ttgctcctta tcctgaagaa cttgaaagaa ggatgttttt tgtagagaca aaaaaaggag
     2521 aaaaagtagc aacctgtacc gcttggcgaa aaaaatcaag agagcaagtg tatccggttt
     2581 ttcattggtt agcagtgaaa ccagattacc aaggaaaagg gctcgcaaaa gcattgactg
     2641 ccagagtctt gcaagaattt cctaccctcc atacggacgg accaatctat cttcacacac
     2701 agacatggag ccaacaggcg attgctcttt ataaaaaaat gggttttact tttattgctg
     2761 aaaatctaga cggcacacct aatccagatt atgaaaaagt tatgcaggtt ttaagtaaat
     2821 tagataaata acgaaatggt gaaataagtc ctcagtattg ggataaaaaa cgaaatagtc
     2881 attcctcata tcttactttt ttgagttaat actatcggat aactaatggc atgccataca
     2941 ttcaaaaaag aaggggcagt gacaaaagta ttgtcactgc cccttctctt gttttttagc
     3001 atgttttatc gtttgatttt tttaggaata aaattcttga tgaacgtaaa tttttggtaa
     3061 tcgtttcgac tatagaggta accaaataca ctgaaaacaa ctgctccaat aaaattaaca
     3121 aataaatcct tcatagtatc catgattccc acgtctaagt aaccattttg gatgattgtt
     3181 ttatctgttt taccatctgc attttgactt tcaatgattg tccgagtgat atctggaaca
     3241 tgatagacag tgtttccgtc tttacttaac tccacactat tgatttgctg gatgatcgta
     3301 tctttttgca tatcgaattg cagtaattta tctgctgaaa attccacaaa ctcccacata
     3361 acaccaactg tcatcgaaaa acagaaggaa acaactgcta gtaataaagg agtcaagttc
     3421 atggtttttg tatttttatt gagcaaataa acaagggaaa agccgacacc cccagctaaa
     3481 aatccgctga atgcatgtag aaaagtatcc cacattgaaa aatag
//
LOCUS       DACEXQ010000238         3515 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.238, whole genome shotgun sequence.
ACCESSION   DACEXQ010000238 DACEXQ010000000
VERSION     DACEXQ010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.238"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..204)
                     /locus_tag="IXH04_002498"
     CDS             complement(<1..204)
                     /locus_tag="IXH04_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1853061.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMK"
     gene            complement(201..425)
                     /locus_tag="IXH04_002499"
     CDS             complement(201..425)
                     /locus_tag="IXH04_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAQ1853062.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            complement(422..715)
                     /locus_tag="IXH04_002500"
     CDS             complement(422..715)
                     /locus_tag="IXH04_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853063.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            complement(753..890)
                     /locus_tag="IXH04_002501"
     CDS             complement(753..890)
                     /locus_tag="IXH04_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAQ1853064.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            complement(892..1338)
                     /locus_tag="IXH04_002502"
     CDS             complement(892..1338)
                     /locus_tag="IXH04_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853065.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            complement(1501..>3515)
                     /locus_tag="IXH04_002503"
     CDS             complement(1501..>3515)
                     /locus_tag="IXH04_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853066.1"
                     /translation="LNGLQMDTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGT
                     FMSEAGYYKHCYVEYRKDNQILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNE
                     TFDAFMAEIKGRVDSLNQQITDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFG
                     DYDYSENPNLMPYITEPWVGPLLGNGHTVKDSVKRVITHTKTRTANSGDILSLGLGIP
                     CTAEANNRYLITTLRPSTTYTLSVTMSVGSDWTGETNTIGVRLRYLNEQGGIELPINA
                     LIPANVERDKMVTHTFTGITKDNVTSITNCYVEIFSLNSEYKGTVSVSYDVKLKAHYP
                     NLLDGPYWLGKVPLGENIADPTVVFPHKTSEYMVYGRRNTENYIADQTYTISMKATKL
                     TVQSFAVYIAAGRVKVGDMKPTEGLANTWELTFTVTKQHIDSGVTNYLEIYQYPSATK
                     GAVQIEWLKLEKGNTRTPNISEYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKI
                     SEITGKANNYTDGKVSEINSQLTASINEVDTTAKDAQTKANANATAIDELDNKIDERI
                     NDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALNGKSSGGNDSTILTIPEGYRPPIS
                     FEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAITYTI"
BASE COUNT          962 a          722 c          584 g         1247 t
ORIGIN      
        1 tttcatgttg gcggctacgt ttcttgctgg tgcatctatt ccagtttcgt gcaaaataat
       61 tttgttagga attgctaatc tggagtctcc ttgattcggc gctaaattat actcgtcatt
      121 aatagtataa gcaaacgtat taatgggtaa taaaaaaaga gccgttaaca ggctcatcgc
      181 agtaatagta attttctttt tcatttgttt cctccttctt cgctttcagc cgagaacatt
      241 ttgtaggttc gatttgatac acccaacaca ctccctaaaa acgcgccaaa accagtaatg
      301 atgacaacac agatatctgt gtactgccaa ttgagcgctt taccaactaa ccccacgaaa
      361 gtagctagtg cgggaataat taccagtgcg aaccatttta gtacttcgaa cgttttatta
      421 ttcattttct tctctcccta aataaagttt taatttgttg cgtgtgttct accaattttt
      481 ctgcatgtgt atctaatctt tcatcgtgtt tctttagttc ttcatgaatc atcaatcgat
      541 ctgatttgct cgattctaaa tctttagtca gcaaatctaa attgtgactt acttttgaaa
      601 gagtctcagt aatcttcgag aaagatgcag taattggttt tattactaat aaaatcaaag
      661 aaacgatagc ggttattgat cctgctatcg ctccccattc ccctaaatta atcatgtgac
      721 aactccttga atcaaaataa aaagcacatc aattaagatg cgctctcttc tttgctaatg
      781 attttatctg ctttttcttc agtaatgcac aacggaacga aaaccattac ttgttcgtta
      841 gtgaaacagc cccaatcata catcattttc acatcgctaa aactaaacat actactcacc
      901 tccctttgaa gctggattta gttgctcttt aatttctgaa atgtctttgc tattttgtaa
      961 cgaagcaagc atcatttttg aattgatttg tgctaaacta tccgcttttt ctttcaatgc
     1021 agtattttcc tgtttaattg ctacatcgct tagcatgagt ttggcattga tctgttttaa
     1081 atcgccgttc tcattttcta acgactcata cattgctttg agattgttta aatcgttgtg
     1141 atctagtgcg ttcgctaaaa taatccattg attcaattta ggatcaaaca tttgatcagc
     1201 gattgttaac ggttcgccat cagcacgaat tccttcaagc ggtggctgat ctgtgtaagg
     1261 aacggataca agcatgtcgt ccaatacttt tcctgcgtac tctccgccag tacgtccata
     1321 tttccaaatg tttttcattt atttccctcc cagtaattga atttcggttt ccaatttgga
     1381 atcggtggtt cgacttctgt gcattcttcc ggtaaatgtt cttcatcatt cacaatgatt
     1441 tgctcgaatc cgtaaggttc aattggtcta tatgctgcct tcatatcgat tcacgctttt
     1501 ctaaatcgtg taagtaattg caaaggtata atccgatcca taacttgagt ttcttcgcca
     1561 tttaatggct ccatctgcac caatagataa ctgagcactg ttcaaagtag aacggtctat
     1621 cgagccaacc agttgctcaa aactaattgg tggccgatag ccttctggaa ttgttagtat
     1681 cgttgaatca tttccaccac tgctttttcc gtttaaagcc acaaaatata tagaaactgt
     1741 ttttccttca cgataaagct ttgctgatcc ggtattcccg tttgtaactg ttaatgtggt
     1801 agtagctgta tcattaatgc gttcatcgat cttattgtct aattcatcta tagcagtcgc
     1861 attagcattc gcttttgttt gagcatcctt agctgtggtg tctacttcat taattgaagc
     1921 agtcaactgc gaattaatct ccgatacttt cccatcggta taattgtttg ctttaccagt
     1981 aatttcagaa attttaatat ctgtggccaa attatcttcg acatattctg gtgctagatc
     2041 ccaaacataa tcttttggat tgtttgaatc acgcatacca gtaccacgat atttatactc
     2101 actaatattc ggggttcgag tattgccttt ttctagtttg agccactcaa tttgaacagc
     2161 tccttttgta gctgacgggt attggtatat ttccagataa ttagttaccc cactatctat
     2221 atgttgctta gttacagtaa aagttaactc ccatgtatta gctaacccct cagtaggctt
     2281 catgtctcct actttaacgc ggccagcagc aatatacacg gcaaacgatt gaactgttag
     2341 tttagttgct ttcattgata tggtatatgt ttggtcagcg atatagtttt ctgtattgcg
     2401 tctaccataa accatatatt cactagtttt gtgtggaaag acaactgtag ggtcagcaat
     2461 attctcgcct aaaggaactt tacctaacca gtaagggcca tctagtaagt tagggtagtg
     2521 tgcttttaac ttaacgtcat agctgacact aaccgttcct ttatattcac tattaagtga
     2581 gaatatttca acataacagt ttgttatgct agttacatta tcttttgtta taccagtaaa
     2641 tgtatgagtg accattttat cgcgttctac atttgctggt attaatgcat taatgggcag
     2701 ttctatacca ccttgttcat ttaaatatct aagcctaacc cctatagtat tggtctctcc
     2761 agtccaatct gaaccgacac tcatagtaac gcttaatgtg tatgtcgtag atggtcgtaa
     2821 agtggttata agatacctat tattagcttc cgctgtacac ggaataccta agccaagcga
     2881 taatatgtcg ccggaattag ctgttctagt tttagtatga gttataaccc ttttaacact
     2941 atctttaact gtatgtccat tacctaacaa aggtccaacc caaggttctg taatatatgg
     3001 cattaaattc gggttctccg aataatcata gtccccgaag tcgatgctgt tactgtacat
     3061 cacttgtaag ttacctaact tagaaatttc ttctttcaga gcatctaact tgtcttgtag
     3121 cgttttagct tgaccagtta aatcagtaat ctgttgattt aagctatcca ctctaccttt
     3181 gatttcagcc ataaaagcat caaaagtttc gttgtacttt cgaatcaact cttctaattg
     3241 cgaaacatat tcatcggctt ggccttgcga aatgtcagac actcctagtg agaaaaaaat
     3301 gatatcttgc gttgttaaaa tttgattgtc ttttctatat tctacgtagc agtgtttata
     3361 atatcctgct tcactcataa atgtgccatc aagagaaaac gtgacttctt cactagttac
     3421 actagttgca acattatcta cgtaacggtt agatggtgtt gttcctttta aagtaaatgt
     3481 tccgccactc gtatccatct gcaagccatt tagaa
//
LOCUS       DACEXQ010000239         3478 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.239, whole genome shotgun sequence.
ACCESSION   DACEXQ010000239 DACEXQ010000000
VERSION     DACEXQ010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3478
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.239"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(155..502)
                     /gene="rplS"
                     /locus_tag="IXH04_002504"
     CDS             complement(155..502)
                     /gene="rplS"
                     /locus_tag="IXH04_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAQ1853067.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(638..1399)
                     /gene="trmD"
                     /locus_tag="IXH04_002505"
     CDS             complement(638..1399)
                     /gene="trmD"
                     /locus_tag="IXH04_002505"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAQ1853068.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            complement(1389..1910)
                     /gene="rimM"
                     /locus_tag="IXH04_002506"
     CDS             complement(1389..1910)
                     /gene="rimM"
                     /locus_tag="IXH04_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAQ1853069.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            complement(2080..2871)
                     /locus_tag="IXH04_002507"
     CDS             complement(2080..2871)
                     /locus_tag="IXH04_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853070.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            complement(2996..3247)
                     /locus_tag="IXH04_002508"
     CDS             complement(2996..3247)
                     /locus_tag="IXH04_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ1853071.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            complement(3259..>3478)
                     /gene="rpsP"
                     /locus_tag="IXH04_002509"
     CDS             complement(3259..>3478)
                     /gene="rpsP"
                     /locus_tag="IXH04_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAQ1853072.1"
                     /translation="IVVADSRSPRDGRFIETVGTYNPLKDPAEVVLKEDLVLDWLSKG
                     AQPSDTVRNILSKEGVMQKHHEAKFSKK"
BASE COUNT          938 a          724 c          629 g         1187 t
ORIGIN      
        1 tttttttact actacttata ggtagattta ttctaatcca ttattctccc ctaccaataa
       61 ttaaagaaaa aactggatca gggtgttcaa attacatgct aagaaaaaag aacccttgaa
      121 taccaagggt tctttctcag tctgataaca atgattaacg acggatttct ttgatacgag
      181 cagcttttcc gtgtaatgca cgtaggtagt acaattttgc acgacgtact ttaccgtagc
      241 gaactacttc gatttttgca acacgtggtg tatgcaatgg gaatgtacgt tcaacgccca
      301 ctccgttaga aactttacgt actgtataag tttcgctgat tccagcaccg cggcgtttga
      361 taacaacacc ttcaaataac tggatacgtt cacgagtacc ttcaacaact tttgcgtgaa
      421 cacgcacagt gtcaccaggg cggaacgctg ggatgtcaga acgtaattgt tcttttgtta
      481 attcttcgat taatggattc atggttttct tctccttatt ccaaatattc ttatgagctt
      541 ttctcacagc ggaatatcgt aatagttaag tgcttttgca ctcaccataa aatactaaca
      601 tattatcaac ctactgtaaa gaagatttta gaaaaagtca cttggcttca tctgctgttt
      661 tttcttgttc ttctttcttg atttcttcta aaaatttcgt catttgaggt gtccattcaa
      721 gtgtttccaa aagatcaggc cgacgttgat aggtccttct tagtgattct tttaattgcc
      781 attcttcaat caatttatga tttccatttg tcaatacttc tggtactttc atccctttat
      841 attctgccgg gcgagtatat tggggatgtt ccaataaacc ggttgaataa gaatccgttt
      901 gagctgaagt ctgattccct aacacatctg gcaaaagccg aactgttgca tcgatcatga
      961 ccatcgcacc taattctccc ccagtaagga cataatctcc taaggaaact tcatctgtca
     1021 ctaatgtgcg gatacgttca tcatatccct cataatgacc acaaatgaat actaagtgtt
     1081 cttcttgtga aaattcttct gccattttct gatcgaatcg tttaccagct gggtcaagaa
     1141 gaattactct ttttttgaca tccggtgcag ctttttcaat ggcttggatg ttatcataga
     1201 tgggctggac tttgagcagc atacctgctc caccgccgta tggataatca tctactgtct
     1261 gatgtttgtt atctgaataa tcgcggaagt tagagatatt gatatctaat aatcctttat
     1321 ctacagcctt accgatgatg gattccccca taggaccttc aaacattctt ggaaataaag
     1381 tcaatacatc aatcttcatc gtcaatcagt ccttctggaa tttctacacg gaccactcca
     1441 gcttctaaat cgacttcttt gacgacagaa gcgatatatg gaagcaatat atcagctttt
     1501 ttcggacgtt gaacgaccca aacatcattg gcacctggtg acaagatttc ttttacttta
     1561 cctaactcag ttccatcttc taatacatgc agaccgatga tctcatggta atagaattca
     1621 ttttcagcta gatctacaga gttgtctttt gacactttta atatgccatc ccgatatttt
     1681 tccacatcat tgatagaagg atgtccttca aaactcaata agtcaaaatt tttatgtttg
     1741 cgatgacttt tgactgttaa ttccacaggt gcttttccgt cttggaatag tgttagcacc
     1801 gttccttttt tgtaacgttc ttctgggaaa tccgttgttg agataacacg gacttcgcct
     1861 ttgatacctt gtgtgtttac gatttttcct acattcaagt attcagtcaa cttatatccc
     1921 catttctttt ttgtctgttt tattttagca tgtgtgaaga ggcaattcta gttttctttt
     1981 aaggtattaa gccaaaatta tgcaaaaatg cggcgtgcta tttttgatta cttcctgtga
     2041 cttggagtta aatgatgggc tcagatcagc gcgctgattt cacaacctct tcatagaaac
     2101 tgccatcttc aacgcagaca taggatcttg atgagtgatc gtataggcat ttttcggttc
     2161 ttgccagctt acttctacgg ctcgattcgt cgtatcaccc atatcaacag taattttacc
     2221 aaattgttca ggtgctggta gactgttttc ttccaaataa gctacgattt ctttagctaa
     2281 tagtaaggga tcttggcctt ctatattatt cccacggatt cgaatacgcc agtttccttc
     2341 ttgccattca agaaaacttg aacctgctgc accttgctga taccctgtga ttcgagaacc
     2401 taagtccact tgctgaccat ttgtgtcgat ctcaaatggc tgcaaaactt ggattgtttc
     2461 ttgactagaa gcaaacccat attgatatag ataggttgca ataggcgttt cttgattcaa
     2521 ttcgtcagca ttcaacccga ggggacgatc tgttctgtaa tataaaatag aataaccttg
     2581 ctttgtatgt gtcgctgcga tattgagata gcctgaagag actggtacat ctagaggcat
     2641 gggaacattt ggctgttgtt ctttcaactg gtctaaagca gtctttttct cattatttga
     2701 ggtagccatt gtttgttcag tggtggaagc cgttttggta ctactttttt ctggactggt
     2761 aacagtttca gatatactgc ttgatagatg tgtattgtct gattcttgag taaccgaact
     2821 acaagcgctt aacaatagag caaatggaat aaataacatt ttctttctca ttggctgttc
     2881 actccctcct cctataagga tacgctttct tgcttgtttt gccaaataat catttcttca
     2941 attaataaaa aaaacaaaaa agctgcactc gaaaattcga gtacagcacg tttatttact
     3001 tttccttatc atcgatgata ttcaaacgca cttttttcgg tgcattgatc cgaacaccgt
     3061 aaacgattgt gcggattgct ttagcaacac gcccttgttt tccaatgata cggccaatat
     3121 cttcaggagc cactgttaga ttgtactcgt aaaagtcttt cgactctacg acgtctaact
     3181 tgacttgatc aggataagtg actagcggac gaacgattgt taagactaag tcgcttaaat
     3241 ctttcatatc cggtcacctt atttctttga gaatttagct tcgtggtgtt tttgcataac
     3301 gccttctttt gaaaggatgt tacgtacagt gtcagaaggt tgtgcacctt ttgataacca
     3361 gtcaagaact aagtcttctt ttaaaactac ttctgcaggg tctttcaaag gattgtaagt
     3421 acctacagtt tcgatgaaac gtccatcacg aggagaacgt gaatcagcta cgacgata
//
LOCUS       DACEXQ010000240         3462 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.240, whole genome shotgun sequence.
ACCESSION   DACEXQ010000240 DACEXQ010000000
VERSION     DACEXQ010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.240"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..277
                     /locus_tag="IXH04_002510"
     CDS             <1..277
                     /locus_tag="IXH04_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HAQ1853073.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
     gene            420..641
                     /locus_tag="IXH04_002511"
     CDS             420..641
                     /locus_tag="IXH04_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1853074.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            657..1499
                     /locus_tag="IXH04_002512"
     CDS             657..1499
                     /locus_tag="IXH04_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853075.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1562..3364
                     /locus_tag="IXH04_002513"
     CDS             1562..3364
                     /locus_tag="IXH04_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HAQ1853076.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYCWKYKD"
BASE COUNT         1250 a          481 c          712 g         1019 t
ORIGIN      
        1 agaactcatc aagctggcta ataagggtca agattatgaa catctagcag atgagattga
       61 tgagctgaga gacaagcgac agctcctttt agtagaagat gcctccctca gtggcgagaa
      121 cgagcgaatc aatgagctga ttgaatttat ccgcaagaac aaattccgta ccttagagta
      181 cgatgataag cttgtaagga agataatcca gagcgttaca gtctatgaag aacacttcgt
      241 catatccttc aaatctggca tcgaaatgga aatatgaaaa ccagaagtaa atagcccatg
      301 attctacagt agagtcgtgg gttttctttt gcttgctgat tataataata ttttaataac
      361 tattgttatt ataaaccaaa tgatttataa tattcttagc gtaaacttgg aggtgctgta
      421 tgactacggc agaaatgatt aaagaactgt gtgagcaaat gaatataagt gtttccgaac
      481 ttgctagacg tattggacag actccacaga atttcaataa gaaattacaa cgagaaacgg
      541 taaccttaga tgagttgaaa gcgattgctg atgtgctagg tgtcaagttt gtgcaggctt
      601 ttatattgcc tgatggcaat gaaataaaga taggtaatta ggaggtgtaa tttgtattga
      661 aaatcataac aataaatgat gttgagtatg ctgtatttgc tgctaatgaa ggtacttcca
      721 aaccacaacc tcatatcata gaaacgaaat ctggtactat tccagaagga aaacaactga
      781 gtttattaaa agaatatctt aagcagaatg atatttcccc aataaaagga gcaacaacat
      841 attggtgcat tgacaaggtt cttaagcttg attcaagtaa agaaaaaact ataagtgaaa
      901 caatccataa acaaaaatac ttatctttaa ctgaagaaaa tattgagaaa cagcataagt
      961 ttgtaggtgc aagctctaat tatgggaaag aaggtctaat aatacatgat gttttgaatg
     1021 catttcctct tcataatgat ttaaatacta ttgcgatgaa gattgcggta attgatgtaa
     1081 caaattcaac acatctttct caatataaga gcaggttgtc tctatatgat ttggctaaag
     1141 taattctgga gattccaaat tttgatgatc ggttggcaaa aggagatcct cagttgataa
     1201 atattatcgc aaggaacatt ggtgcagtta atatgttctc atttgcatcc aagtactgta
     1261 catatcataa tgtggaagtg tgtgggagag atgattactc catctttgat ggtatcgtta
     1321 aaaatactct tccacactat atccaaggac taactacgaa taagattgat acgtggcgaa
     1381 gaagttttga ttacgaggca tttaacgaat gtgttggaaa actacttgat gaaaataata
     1441 tacatattcc ttttagaaga agaaagttag atcacttctt gtggtatgcg aataggtaga
     1501 atataagaaa aaggcatcgt aaaagtgatt ctggataaaa ttaatggggg aggaaataat
     1561 agtgggatta tttgattttc taaagaaaaa acaaggaaag gttagtaaac acgttgatta
     1621 tcaactggct tctagtctat ttcgtactgg taaagttttt gctgaaaaat tggaagtgga
     1681 catatcaaat gtagatgagt ttcgtaaaac ttttatcgct tttgatgtgg aaacaactgg
     1741 cctaaattct tcttcagata gaattgtaga gattggtgcg gtgctattta aagaaggagt
     1801 tccaattcaa tcattcggaa ctcttgtcaa tccaaaagtt agaattgcag cctcagcttc
     1861 ggcaataaat aatataacta atgaaatgct aaactctgct ccaaatgagc aggaagttta
     1921 cccccagttg gtagaatttt taggagatgc actagatgga aaaatcacta tgtgtgcaca
     1981 caatgcaagt ttcgattttg gatttttaag taacacattt tctagacttg ggtatgatgc
     2041 taaaattaag tatgttgata ctctgagtct ctctagaaaa catataaaag gcattgtaaa
     2101 tcataagcaa gagactgtag cggaatattt cggattaata aatgacactg cccatagagc
     2161 ggaatcagat gcagatatat gcggaaagat tttttggggt gttttagata ttattgagga
     2221 gtcgttggaa gaacaaaaaa agcaaataga aaagatgact ccagacataa aagaacttga
     2281 agcttgtgct tttattcaag atattattga gaaaaaagga tgcgacacca gttggatcag
     2341 gtatcgaaaa aacagtaata attatgtcga tgtaacttgt ctatatacat tcttaaagtt
     2401 taagtttgct aagaagggaa attacatcat tattagaaaa gatgctacaa atggaattga
     2461 tttacctata gaaccttgta ctgtaagtga aggcggtaca acaaatgtgc gagtctattt
     2521 tcgtagcccc tttgacttaa cgcctctttc aaattatatt ttcagtgctt atagcgattg
     2581 ttataattct atgaatgaat atatgagtgt tagtaattac actaaaaaag aagcagagca
     2641 aagccttagt atgctgaaat ctatttcaaa atcagcgatg aaagagttat tattcgatgc
     2701 aaaaaatcgt ggatatgatg agactgaagt taacctaaat attgaaaaag tgatatctag
     2761 agaagatgta attattaacg ggttgcataa tcgagtttca ttaaacaaga tacgaaatat
     2821 agggaattgg gagaaaggat ttgatgcagg ttatccatat tgggaacgtg gagaaactgc
     2881 taggaaaaat ggcaaagtag atgaagctat tgcacttttt gataaagctc gatataacgg
     2941 ctatgatgct cctgcgctat atgattctta tgctaaagct tatcgaaaaa ttaaggatta
     3001 cgataatgaa attctcattc ttgatgaagg aattatgcgt aaaacacggc acgatgttgg
     3061 tactttagag gctcgtaggg ataaggcaat taagctatta tttgctaaac aagaggcaga
     3121 gcggatagct aaggaaaaat ctgattttct aaaagcgaac aaaaaagagg atgcgattag
     3181 tgagcctagc aagaagcgtg gaagacagat tattcaaatg actgatgatg gaacaataat
     3241 aaaagaattc gatactatag cttctgctgt aaaagaagtt ggcgttaact caaaaagtat
     3301 tagggatgct gcaaatggag ttcaaaaaca tgctggagga tactgctgga aatacaaaga
     3361 ttagcagata atctaagata acctaggatc ttcttaatag atacccgtgg tgctagttaa
     3421 ttttaatatc ctaaagaata tctcaaagtt ccttcaaaac gt
//
LOCUS       DACEXQ010000241         3429 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.241, whole genome shotgun sequence.
ACCESSION   DACEXQ010000241 DACEXQ010000000
VERSION     DACEXQ010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.241"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            9..827
                     /locus_tag="IXH04_002514"
     CDS             9..827
                     /locus_tag="IXH04_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1853077.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            1057..1287
                     /locus_tag="IXH04_002515"
     CDS             1057..1287
                     /locus_tag="IXH04_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAQ1853078.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(1510..1608)
                     /locus_tag="IXH04_002516"
                     /pseudo
     CDS             complement(1510..1608)
                     /locus_tag="IXH04_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(1873..2328)
                     /gene="tnpA"
                     /locus_tag="IXH04_002517"
     CDS             complement(1873..2328)
                     /gene="tnpA"
                     /locus_tag="IXH04_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAQ1853079.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            2571..3429
                     /locus_tag="IXH04_002518"
                     /pseudo
     CDS             2571..3429
                     /locus_tag="IXH04_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         1136 a          586 c          596 g         1111 t
ORIGIN      
        1 gagtttgtat gagttatatc tatttaatta aagaaaatta tccattttta acgaagtctg
       61 aaaaaaaagt cgctgatttt attttaaatt ctgggaaaac tattatttat agtacgatga
      121 gtgatattaa gaaaaacaca aaagttggcg atgctactat tattcgcttt tgtcaaaaaa
      181 ttggtttttc tggtttttct gatttaaaaa ttgaaattgc aaaggaagac tttagtcaaa
      241 aaaaagaaca tccgactagt gtaaagtact atgatgaaat tgctaatagt ttaacagaag
      301 ctctctattc cactattagg cttttagacg aagaaaaatt gaatgaagcc attaaaatga
      361 tcactcaatc aaaaaacata tatatatttg gagtgggatc tagtggaaat actagtcttg
      421 atttagaaaa tatgtttctt cgcgtaggag ttcaagcaaa agctgtacta gaccctcatt
      481 ttcaagccca agtcgcttct ttattaacag ttaatgattt agtaatcatt ttttctttat
      541 ctgggaaaac gaaagatact tatgattcat tgaaaatagc taaaaaaaat ggcgcaaaaa
      601 ttattgctat aactaactat attcattcac ctataggaaa gtctgcagac ttagttttac
      661 aaacagccat tgaagaattt cttaatggtg gatctttagc tggaaaaata tcacaacttt
      721 atatttgtga tctattagtt catggttatg aacaaaacaa taatattgat tcagttgaat
      781 taagagaaaa agttttgcgc tctattattg ataaaagtat tgagtgaact acccccactt
      841 agctaaacct aacggttttt aacgattgaa gtaggcaatt cttgggatga gagcatactt
      901 gatagcttat tttttactaa tagcggtggc tcttattgac caaatatccc tgtagttcct
      961 acggttcttg attttatcta aagatatttt tatcgtggaa gctattgatc aacttgaaag
     1021 aaaaacgtgt agctcattat cactagttta tcgagtatgg atgaagcaat ggatcatcct
     1081 ctcttaaata aatttattaa ggatctgatc cttcaaattt tttccatgat cgatgagcag
     1141 gaacctactg aatctaaacg acatcgggca caaggaatta ttaagattgc taagcgaatg
     1201 ttttctataa agggtgttct aagctataca gtgttaattc aaaagattcc caatcatctt
     1261 gtttataaga acaacgttga agattaaaaa agtgtttcaa tgctaaaatt gcaaaatact
     1321 tcaatgttat gagacaaact gtctataaga atcaatttcc acccatttca tcacttgtta
     1381 aaagtttatg atccttttga tttttatatt aatttatctg tatttaatac agattttatt
     1441 tattagttca tctgcctatt gtattttatt ttgttgcctt accatctgaa gtaaatatac
     1501 tcttaattgt caaattaagt tagacagatc atcaatactt acgtaactca aaaatacttc
     1561 caaaggtgtt cgataatcca gtgattttct aggaagatta tttctgcgcc agcattttct
     1621 aatgtttgaa tttgtgtttt gtaatcttgg ttgcttgtgc taattcttga atagccgtaa
     1681 attgccaaaa tttcatcacc ctttctggac ataagtttct gacaccagtt aaaatcaagt
     1741 ctatcagtaa cccatacacc cgtgaggacg ggtgtatttt taatccccct agaaggggtc
     1801 gacactcgct gccccttaaa aggggatttt taaaagaccg ccaactgctc atcttcactc
     1861 acttacttct ctttattttt taaatggttc tacgtactct cttttactta taatatatcg
     1921 aatgcgatca tcagcttctt gttcacggat gtatttttca tttgtctttt gattcaatcc
     1981 gactgtactc acatagtatc tcttcgacca aaaggatttg tttccgtatt tatatttgag
     2041 atttgcgtgc tgctcgtgga ttagtaccgc actacgacct ttcagatacc ccataaaact
     2101 ggatatacat atttttggtg gtactcgaac aagcatgtga atatgatcta gcattgcatg
     2161 tgcttctatg atttccacat ctttcatttc gcacaacttc ctaagtattt ttccaagatc
     2221 ttttcgtaat tgcccataga ttacttttcg ttgatatttt ggtgtaaaaa taatatgata
     2281 cttacaattc caacgagtat atgctaaact tttatcgtca tttgacatga aaaaactcct
     2341 ttcgatctga tgagcggttg gtagccactc actagtatat cgaaaggagt ttttatcgct
     2401 aaagatactt cttccacgcg tagaaacgcg cggttttctt gtttcgcacg ctttacgcac
     2461 aaaactgact gaagtcatta agaaaataaa agggtctaaa acttttaagt tttgtaccga
     2521 cccccaaaag ttagaccaaa aaatctaact tttggaggtc ggtatttttt atggctaagt
     2581 atacttttga acttaaatta aagatcgttc atgattactt ggatggtaaa ggcggaagtg
     2641 attatctggc aaagaaatat tccattaagg ctccatcaca agtgaagcgc tggatcaatg
     2701 cctatcaaga atttggtgaa gagggtctag taagaaagcg acaaaagaaa aaatattctg
     2761 ttcaatttaa gctggatgcg atagagttat atctaacaag tgagctgtcc tatcgtgaag
     2821 ttgccaacac acttaacatg aacaacccta atttgatcgc tagttggatg cgtcaattcc
     2881 gtgagggtgg gatcgatgga ctctcaaaaa cgaaaggatg tccacctatc ttgtctaaga
     2941 aaaatgaacc caaaaataag aaaaaatcta caactaaagc aacgtctaaa gaacgtgaac
     3001 gcatcgagga gttggagaaa cgagtccgtt cccttcaaat tgaaaatgcc tttttaaaag
     3061 aattgaggaa gctgcgaaaa caggaagccc aacaacgacg aatgaagcaa tcgcacgaat
     3121 catcgcaagc ctccgaggac cgtttaaatt agttgagctt ctagaaactt taaagttccc
     3181 taaggcaatc tttatgtact ggcaaaaacg attggaccgt aaaaatccag atcaagagat
     3241 tgaagcggaa atgcttaaag ttcgggagaa gcacaaggat tatggctgtt tacggatgac
     3301 caatgaacta agaaaccgcg gtttctctat caataaaaag aaagttcagc gcctgatcaa
     3361 gaagctagga ataaaagtaa cgacatacac acgtaaatca cggcgctaca attcttatcg
     3421 aggacaagt
//
LOCUS       DACEXQ010000242         3394 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.242, whole genome shotgun sequence.
ACCESSION   DACEXQ010000242 DACEXQ010000000
VERSION     DACEXQ010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.242"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(49..1515)
                     /locus_tag="IXH04_002519"
     CDS             complement(49..1515)
                     /locus_tag="IXH04_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAQ1853080.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1902..2498
                     /locus_tag="IXH04_002520"
     CDS             1902..2498
                     /locus_tag="IXH04_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ1853081.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(2556..3038)
                     /locus_tag="IXH04_002521"
     CDS             complement(2556..3038)
                     /locus_tag="IXH04_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853082.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3144..3341)
                     /locus_tag="IXH04_002522"
     CDS             complement(3144..3341)
                     /locus_tag="IXH04_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853083.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
BASE COUNT         1150 a          689 c          562 g          993 t
ORIGIN      
        1 aaacaggaac tgctgcaaat aagcaattcc tgtttttatg ttcattgatt aatgaataga
       61 gcccattagt tttttgattg gttctttcac agtaaatagc aagataccac cgacgattgc
      121 tactgcaccg ataataccga agtaagcaga ttctgttccc ggtgtgtaga agcgtgcaat
      181 ttgtgcattg attgcttgtg aagaagcatc agctaagaac caaatagcga ttgtttgtga
      241 ttcaaatgct cgtggtgcta attttgttgt cacagacaaa ccaactggtg aaagacacaa
      301 ttcagcaata accacgatga agaaacttcc caacaaccac aatggactta cttttccact
      361 tgtaccataa agcatacccg gcaacatcaa tagcaagtac gataatcctg caaataaaag
      421 tccaagagag aatttcacta ctgtagatgg ttgacgttca ccaagtttcg tccacatcgt
      481 tacgaatact ggtgtcagaa taaagatgaa aattggattc aatgattgat accagcttgc
      541 ttggatcgtt acaccaaata gcgtagattg tgtacgttca ctagcaaata aagctaagat
      601 agatgaacct tgttcttgta atgaccagaa aatgattgct gcaaggaata atgggatata
      661 agcccatact cttggttttt cttcagcagt tacatctttt gaagtcagca ttttgataaa
      721 gtaatacaac ggtaagataa tccccaatac actgattgta ttgacaaaaa agtcaattgt
      781 cagatgacca gttagttgtg ccccgccgaa tactgcagcg ataacaacaa gtgcgatgat
      841 caagttacgg atgaatcctt tacgttcttc agttgtcaaa ggattcggcg gtgttgtacc
      901 cactccgtct aatgtcttgc gtccttggat gatgtattgt aataatccaa agaacatccc
      961 aatagcagct aaagagaacc ctaaatgata gttatattct tgaccgattg taccaacgat
     1021 tagtggtgct aataatgccc ctaagttgac acccatgtaa aaaatcgaaa atcctgcatc
     1081 tcttcgcaag tcagtctttg aataaagatg tccgaccatg ccagaaacat ttggtttcaa
     1141 aaaggcggtt ccgataataa tcaatgccat cgaaacaaac aatgtactta caccaaatgg
     1201 taaagccaag acgatgtggc caagcataat gaaaactcca ccgaagaata cagtgcgttt
     1261 tgctcctaac actcggtcag atacccatcc gccgacgata ctagacatgt agatcaaaga
     1321 tccgtagatc gacatgatag ctaatgctgt tgctcgtggt aatccaagtc ccccgttggc
     1381 aactgtatcg tacatataat agataagtaa tgcacgcata ccgtaataac taaatcgttc
     1441 ccacatctca gtgaagaaca aagttgacaa tcccttcggc tgcccgaaga agcccttgtc
     1501 ttgctgtttt tccataatct ataccccaat tctttaaatt ttcagaaaat attatacatc
     1561 tttaccgaaa aatttaacta aaaaacttat ttaagaaata taaactaatt ttctataaaa
     1621 gcaagtaatt tttaatggca atatcataaa aaaaagaaga tagagattta ataattatat
     1681 atcagctgtt tttttcaaat aagcaaaaat tcctaaaaca caacaatgaa agcgataact
     1741 aagaaaatcc ttacataacg atttttctta ttaaaattgt tttttgaact tcgcttattt
     1801 tttgtgagca tatctgttta cttacttttt gtaaggtgct atacttcatt tcgtaatcaa
     1861 ttaaaaaaat gcaaaacgca aatcaaagga gaaattttat tatgacaact tttactgaca
     1921 ctttgaaaaa ccgccgttca atctatcatc taggacgtaa tgtttcactt tctaatgaag
     1981 aattaacaac tttgatcaaa gaagcaatta aagaaagccc aactgctttc aatgctcaat
     2041 caacacgcgc agttatcctt ttcggagatg cacatgaaaa actttgggaa atcactgaag
     2101 aagcacttcg cccattgact ccagcagaag ctttcccaaa tacacaaaac aaattagctg
     2161 gtttcaaaaa tggttatggt acagtattat tcttcaaaga tactgacgtg gtaaaaggtc
     2221 tacaagaaca atttgaatta tatgcagaca acttcccaga ttggtcagaa caatcaaacg
     2281 gaatcgcaac agctaatact tgggtagcat tagttgataa aggattgggc gcaaacttgc
     2341 aacattacaa cccagttatt gacgaagcag ttgcaaaaga atggaatatc ccttcaaact
     2401 ggaaactacg ttcgcaatta gtattcggtt caccagaaac accggctggg gaaaaagaat
     2461 acatgaacga tgccgatcgc ttccgcgtat ttggataatt ttatttgaaa taaaaaacag
     2521 aggttgtgac aaacatcttg tcgcaacctc tgtttttatt ctggatcaat cataatatca
     2581 atctctgcct catcgtttaa gcgttgggac aaaaatggaa ctaattcttc taatgatgca
     2641 gaaaatttca gcatcggtcc tcgccatccg tttcggaaaa aattcatttt tacgatttta
     2701 gaaccttttg gctgatataa tacaatttta ttgatctcag aaaaaggaac accttttttg
     2761 tcaaagctat tcaaaacaag tccttcttct gtgattcctc tactgtccaa caaaaaacta
     2821 ataaaaatca ttgctgacaa gatccctttt gccaattcat ctgctgtagg atgactgatg
     2881 agtgtatacc aaatgactag tgacactaag aagggtatca ccaagtagac ccatcgattt
     2941 cgcacagtcc gataaataat ttcttttcga accagccaaa gaaataaaaa catcacaaca
     3001 acgaataaaa acaatagaac cgtaaagaat gttaccatga caactcgcct cgctttttct
     3061 tctccagaaa ataggaaaag aatactgatt taccttctca aagaaattgg acttgcagca
     3121 cgccgcacgt ccaattgttt tctttattca aatgtatcat attttgcaac aaccatgctt
     3181 gctttttcat ctaataattc ttggtcctct gcagcacatt gggcaacttt gaccacggcg
     3241 cagtttgtca tttttgatac aacttcacca atgaccgttt ttgtaaaacc aattggtttg
     3301 catgcatatt gttctcctaa attaatcatt tcttccgtca tcaaaatcct cctaaactgc
     3361 tggaactttt ctccacatta ccactgctat ttct
//
LOCUS       DACEXQ010000243         3390 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.243, whole genome shotgun sequence.
ACCESSION   DACEXQ010000243 DACEXQ010000000
VERSION     DACEXQ010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.243"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            647..964
                     /locus_tag="IXH04_002523"
     CDS             647..964
                     /locus_tag="IXH04_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAQ1853084.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNSSIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(1055..1420)
                     /locus_tag="IXH04_002524"
     CDS             complement(1055..1420)
                     /locus_tag="IXH04_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853085.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1628..2110
                     /locus_tag="IXH04_002525"
     CDS             1628..2110
                     /locus_tag="IXH04_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853086.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            2307..3197
                     /gene="pstS"
                     /locus_tag="IXH04_002526"
     CDS             2307..3197
                     /gene="pstS"
                     /locus_tag="IXH04_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HAQ1853087.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT         1172 a          519 c          619 g         1080 t
ORIGIN      
        1 ttattgcttt tgcaaaattt atttacctgt tttctggatc aacgatagtg tacattatgc
       61 tatccttgtt tggattttag gacgtttaaa gaggcacttt tttacataac ttaatccata
      121 gaccttttaa gcatgctgtg agataataat aaagaagagt ttaaagcgca ccccaaacca
      181 cttccccata agtgtattac gctttaaact cttttatatt tgaattcact aagaagcata
      241 ccatatattt gaatttacta agaagcatac catatttttg aaaaaaagtg agaaaaaagg
      301 cttataatta ggatttagag taattaatta gatgtttatt ttataggact gatactatag
      361 attacaaata gtatcgtatt tcgcaatctt aaaattatct tcttactgtg aataagataa
      421 taaaatttgt atttgtcctt tttaggtaac ttattttaat ctatatacct ttctggatca
      481 acgaaagtat actttatata gtcataacgc cgatggtaac tatgcactgt aacgataatg
      541 ctgctaaaat atctttttac gaaaaacatc acaaggaaaa acttaactat ggtattatct
      601 aaacataatt ttttcggtat ttaatattta attaggaggt aatattttgc ttttttatag
      661 taattttatc ttaatagtag cgatattatt acttctgaat atatggattt ttgataggag
      721 tagaaattca agtataggtt ttagaactaa acgaagtttg tcttctaaaa aaaattgggt
      781 atactcacaa actatttttt atggaggaat tgttttaatt agtttacttt cttcaacact
      841 ttattctttg aatattatag atgtttccac ctcaaattca atatctatta ttggaatcat
      901 tattgctgca attattacac aactgttttt agtgtttgga gagaagaaaa gaagtaaaaa
      961 atagaaaaac tagtgatagt tttggtgata taagaaaaac atatatgtat aaaaagaaga
     1021 aagtagcttc tctttgtaca tatatggttt gtttttatgg atgccgagct tttttataag
     1081 ataaggctac cgcccataac gacccaagga tttgtggaac taagtaaata cctgcaagaa
     1141 gcaagatcag ctgggtcggt tttaaaggat gatagaagta acgccattca gtaaaattgg
     1201 cgcaaaataa ataggaagtg acagtagata taatattggc aatgaataaa gatataatac
     1261 gcctgttcct ttgaaaataa tatccagcaa aaagagataa aaatatcaat gtgatgaacc
     1321 cgcttacgga atgataacga aaatcaagca gtaaggcgaa atgcacataa ggaacaaaag
     1381 ataaaagcaa cggaaggttt ttccagcaag tttttatcat ctttcatcac cacctaacaa
     1441 gtgttattat tttgttagtt ttattgtaaa ttgaatcctg caataatcaa ttactttttc
     1501 attatttcta gattaaatga ataaactctc atgccattat ctctaaaaaa aaagtttttc
     1561 cccctaattt ttttagatag aaaattgtat agtatgatga gagaaaaagt agtttggggg
     1621 aacgttaatg agaagaaaag cagtaatcga tcggctttat ttattttttt ggttttttcc
     1681 tatcgttttg ctacctttta catgggtaaa tgctgttgta caaggtgcgg agatattagg
     1741 cctgatccaa ggaagcggat ttgtggtttc taggaatttt ccagctttga ttgttttcta
     1801 tttagtgata agtgtaggtc tcttattacc aaaatctctg atcaatcact acaattggca
     1861 gctgctttac ttttcttgct tattagtatt tacatctatg ttttctttgc tgcctcgctt
     1921 gattatcggc cctaatggtt tgagcgagag tggattgaag gatttagctt attcctccat
     1981 gtatttcttg acgatcaaac catggttcta tttagtaatt gcctcattta ttcttgcaac
     2041 atttttttac ttctggacag aaatcaggat aaaaagtgaa cgatctgtat ctataaaaaa
     2101 taactattga tggtggatca tcagtaaaaa aacatatgag ctgcgtaaaa tgcatgtttc
     2161 tcatatgctt tttttattat tatgtaaaga atgcgaaaaa cgtttacatg atttttacat
     2221 aacttaacta tatttttaca gcaaccatac gagggattta cagtcgactg ataaacttta
     2281 gttgtaaata agaaaggagc aataaaatga aaaaagtatt gtttactcta ggtatgatgg
     2341 gaattctttt agccggatgc ggctcaggat caggaaatac agatggctct gccacacaaa
     2401 ataattcaag tgactcaaat gaacaagtaa aaatcgttgc ggtaggttcg acagcattac
     2461 agccgttagt cgatgcagca caagaaagct ttgtacaaga aaatccaaat tatcaaatct
     2521 ctgtacaagg aggaggaagc ggaactggtt tgagccaagt cgaagctgga gcagttacta
     2581 tcggaaactc tgatgtattt gcagaagaaa gagacggagt tgatgcttca aaattagtgg
     2641 atcataaagt agcagtggtc gggatggcac caatcgtcaa taaagataca gatgtcaaag
     2701 atatcaccaa acaggaattg attgatatct ttacaggaaa aatcactaac tggaaggaag
     2761 ttggcgggaa agatcaaaaa atcaacgtcg tgaaccgtgc aaacggcagc ggtacccgcg
     2821 cgacattcga aaaatgggga ctggatggtg ctacccctgt gcagtcccaa gaacaagatt
     2881 catcaggaac agttcggcag ctggtaagtc aaacaccagg agcaatcagc tatttagctt
     2941 tctcatatct cgatgattcc acacaagcac taagtatcga cggtgtagaa ccaaaagaag
     3001 agaatgtagc agataacagc tggggaatct ggtcatatga acatatgtac acgaacggaa
     3061 aaccttcccc tgaagtccaa aaattcttag actacatgat gacagaagaa atccaagaag
     3121 gacctgtcaa agaattgggc tatttaccaa tcacaatgat ggaagtagaa cgtgatcacg
     3181 aaggaaatat caaataacta gcagataaac atgccacggt agtcgttact attaaggaaa
     3241 aatcaactat gtttccccac atatacattt ttcctttaat tgtgaaaaat agttcctcat
     3301 attttgccgt tttttgagct aataccgagg gtcagctgat tgaacaccgt ttattcgaaa
     3361 aaaagagcta cgacatagaa ctgtcgtaac
//
LOCUS       DACEXQ010000244         3376 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.244, whole genome shotgun sequence.
ACCESSION   DACEXQ010000244 DACEXQ010000000
VERSION     DACEXQ010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.244"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(326..688)
                     /locus_tag="IXH04_002527"
     CDS             complement(326..688)
                     /locus_tag="IXH04_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853088.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAEMGKALRKH
                     FGFESFDDFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYLNESDGLFEVIPNNK
                     SQMSESKFTSMFSDVDLR"
     gene            complement(692..1000)
                     /locus_tag="IXH04_002528"
     CDS             complement(692..1000)
                     /locus_tag="IXH04_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853089.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLKLPAEDNYSEMLERMMDVL
                     NSTINAGLAKSSVLTNEFVHRYLAQTTDEAEKEFWLKLMSHVMQLNQISVINSKEKGM
                     "
     gene            complement(1101..1335)
                     /locus_tag="IXH04_002529"
                     /pseudo
     CDS             complement(1101..1335)
                     /locus_tag="IXH04_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1332..2306)
                     /locus_tag="IXH04_002530"
     CDS             complement(1332..2306)
                     /locus_tag="IXH04_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAQ1853090.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLSVPFMDTPLKIQWSIDRITIVG
                     NLKENIYYHTTHDVLILDFEQLMRLNEGNGYLRSVSNNGWQLLDQHEENIAYIEILKW
                     QEGKGRIDFNPNKISQFLASSMKNFIHDLFIEPHFSRADIACDIIDVPDEFITQYRVV
                     DPVSFKPIYGRNGKLETAYWGSRSSERQIRMYNKKLEQEKKRKIVPKEIVSWWRLELQ
                     LRRGKATDWHAMVQESLDSFASPHYLPADTSVADKMMITALTTEHDYWGQINRKTKYK
                     YRNLLKQESQNDELTNHLRETFAESADDLKKELDTWLLGLDVTEEEEK"
     gene            complement(2755..>3376)
                     /locus_tag="IXH04_002531"
     CDS             complement(2755..>3376)
                     /locus_tag="IXH04_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAQ1853091.1"
                     /translation="EMRYISKQREKNEREKALVASEKAKIEEEKKSIIDLEKELAVVF
                     QKDPVPLTSFEKLPDGSYRLSSADFVDLYQKASNATNSKKYADMANESLGNTLDKNIA
                     LFDKNMALEQENSFLKNQQKVDLSAVVAENKKLKEENRSLRQRLTQLKNAFQKSITRL
                     SIRFGLVEKDMGISEELKILDQEKTLASQQNAPERNIGRSENEMEW"
BASE COUNT          965 a          750 c          481 g         1180 t
ORIGIN      
        1 tgcttatgcc aatcattggt tttctggcga tcagttaact cacgattatt atgagaaata
       61 cttgtattct tcgttccttt tttaaatgaa atactaaacc ccaagttaaa accctccttc
      121 gcgctgtcgc taataaaaag agtgtaaacc tttctgttac tctttgtcaa agagtaacat
      181 tttcctaatt gtgacatggc tgtcacaatt ccaaatgctc tccgagggta ttcgctgtca
      241 caagtgacaa cgaaaaaagg agccgaaagc gtgtcacgct ctcaactcct tttacgcttt
      301 attttgcttc acgcaaaata atcgcttatc gtaaatccac gtcactaaac attgacgtaa
      361 atttactctc actcatctga cttttgttat tcggtatcac ttcgaataag ccgtcagatt
      421 cgtttagata atctcgttcg cttggcgctg atttttcatc agcaagccac tcattatcca
      481 ctgatttttc gtgttgctga tcgtcagaaa ttgacgataa ccaccgctca aaatcatcaa
      541 aagattcaaa tccaaaatgc ttccgaagtg ccttacccat ttcagcattt tccttctcaa
      601 tcaatcgttt tttctgctga cgaagttttg cctgttcttt tttcaatttt tcttcttgtg
      661 ctttaatatc tgctaaagtc atccccataa actacatccc cttttctttg gaattaataa
      721 cggaaatttg gtttaactgc ataacatgac tcattaattt caaccaaaat tctttttcag
      781 cttcatcagt tgtttgcgct aaatacctat gaacaaattc attagtcaaa actgaagatt
      841 ttgctaaacc ggcattgatt gtggaattta aaacatccat cattcgctca agcatttcac
      901 tgtaattatc ttcagccggc aatttcaaat aatcagctaa ccgctcatac atcaattcaa
      961 ttctatcaac tgtctcttct cttaaatcaa ttttatccat gttacacctc atttccaaaa
     1021 ctgccaccat ttccgattct ttaattttcg atacacctaa ttcatcagca agatttttta
     1081 tggtcatcct ttctgacaaa tcagtcatta gcttctccac tactttcagc taccgcataa
     1141 atatatttaa cggcgtgctt agggataata atcggagagt caattttatt atttttcaaa
     1201 atcaattcat attgcgttac atcaagtaac ttaccggaca tttttttgct atccaaaaaa
     1261 aacaaccaca acgtaatcac ctgttaaatt taccaaccaa tttttcttac caaaatactt
     1321 ttgtttttct gtcatttttc ttcctcctct gttacgtcta gccctagaag ccatgtgtct
     1381 aattcttttt ttaaatcgtc tgccgattct gcaaaggttt ctcgtaaatg attcgtcaat
     1441 tcatcatttt gactttcttg cttcagcaaa tttcgatatt tatattttgt ctttcgatta
     1501 atttgccccc aataatcatg ttctgttgtt aaagcagtaa tcatcatttt gtcagctaca
     1561 cttgtatcag caggcaagta atgaggacta gcaaaactat ccaagctttc ttgaaccatt
     1621 gcatgccaat cagtcgcttt ccctcgtctt aattgtaatt ctaaccgcca ccaactcacg
     1681 atttcttttg gcacaatttt tcttttcttc tcttgttcca gttttttatt atacatccga
     1741 atttgacgtt cagaagagcg agatccccaa tatgccgtct ctaattttcc attccgtcca
     1801 taaataggct taaaagaaac tggatcaact acccgatatt gtgtaataaa ctcatcaggt
     1861 acatcgataa tgtcgcaagc aatatctgca cgtgaaaagt gaggttcgat aaacaaatca
     1921 tgaataaagt ttttcatgga actagctaag aattgactaa tcttattcgg attgaagtct
     1981 atacgccctt tcccctcttg ccattttaaa atttcaatat aagcaatatt ctcttcatgt
     2041 tggtctaaca actgccaacc attattacta acagatctta aataaccgtt accttcattt
     2101 aggcgcataa gctgttcaaa atctaaaatc aacacatcat gtgtagtgtg ataatagata
     2161 ttctctttaa gatttccaac aatggtaatt cggtcgattg accactgaat ttttaggggg
     2221 gtgtccataa aagggacact taaccgcctg ttagaggggg ctgttaattt ttcccactca
     2281 tttatgatcg tgcctttttt actcaaattt agttcctcct caaagaaaaa agagattggt
     2341 ggaaagaatt ttccagagtt cacgtaactc taaaaaattc ataaccagca atctctagtc
     2401 ttgaagaaac ctgaaattat tcgtataatg aatttgagtt acggagctag tgtgattagt
     2461 ttccgtaaag gtcgagaacg tgtccagcgt tgctcgactt tatttttttg ctcttttttc
     2521 agtttattta tttgataact caataatagc acttctcttt agctatgcaa atatccaaaa
     2581 acataaaacc gcaccctttg aaaagggcgc ccccaaactt cgctaacgct aagttggcca
     2641 aaaaatggac ttgccatttt ttagcaaatg tcagcttcag ggattagtgg tttagccgtc
     2701 aactccttat cctcggcaca gaccgcctcc ggtaccgttg actgcaccgt ataactacca
     2761 ctccatctca ttctcagaac gccctatatt acgttctggg gcattctgct gactagccaa
     2821 cgttttctct tgatctaata tttttagctc ttcagatatt cccatatctt tttctacaag
     2881 cccaaaacga attgataaac gagtgattga tttttgaaat gcgtttttca gttgagttaa
     2941 ccgctgacgc aaagaacgat tttcttcctt cagctttttg ttttcagcaa caaccgctga
     3001 caaatcaacc ttttgttgat ttttcaaaaa agaattttct tgttctaacg ccatgttttt
     3061 atcaaataaa gcgatatttt tatctaatgt atttccaagc gattcattcg ccatatcagc
     3121 atattttttg ctgttcgtag cattagacgc tttttgatac aaatctacaa aatcagcact
     3181 agataagcga tacgagccat ccggtaattt ttcaaaactc gttaaaggaa cagggtcttt
     3241 ttgaaaaaca acagccagct ctttttccaa atcgataatc gattttttct cttcttcaat
     3301 tttcgccttc tcagaagcca ccagagcctt ctcacgctcg tttttttctc tttgcttaga
     3361 aatatacctc atttcc
//
LOCUS       DACEXQ010000245         3361 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.245, whole genome shotgun sequence.
ACCESSION   DACEXQ010000245 DACEXQ010000000
VERSION     DACEXQ010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.245"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..190)
                     /locus_tag="IXH04_002532"
     CDS             complement(<1..190)
                     /locus_tag="IXH04_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853092.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEA"
     gene            complement(207..398)
                     /locus_tag="IXH04_002533"
                     /pseudo
     CDS             complement(207..398)
                     /locus_tag="IXH04_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            506..1732
                     /locus_tag="IXH04_002534"
     CDS             506..1732
                     /locus_tag="IXH04_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003555353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAQ1853093.1"
                     /translation="MRKDVYEGVRFFMINDIKPNFNELGRQYNCDPRTVKKYYLEGSE
                     NSVKKDKDKPKRPSKLDPFKEIIDQKLELNCSAMAIFKFLKKIGYQGGYTIVKDYCRN
                     KKHTEIKKATLRVETVPGTAAQVDWKEDMTLHDKFGKSYTFNLFLYVLHFSKLKFITL
                     TWDRKQDTLFSCLYEAFNHTGGVPKEIWFDNMKTVVDRARTQYRKVVFNTRFYEFSKD
                     AGFEAIACRSYRPQTKGSVESLAKLVERLKPYDYEFYDAVDLISLVNTLCYDLNYEEI
                     SQSTYQRPVDLWEREEKGKLHPVNEALLTPYFEDNIVRIVTNESMVNFRKSKYSVPTK
                     YIGCEVEIEIDPTEKFIEIFYQGEVVQSHELTGKRLNYKETDMFEILKSDVMKHKDDE
                     EIYEYIQRTLTQYDEL"
     gene            1748..2506
                     /locus_tag="IXH04_002535"
     CDS             1748..2506
                     /locus_tag="IXH04_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ1853094.1"
                     /translation="MTTNHQKVLTNLDELGLLKMKEYYPHYLDSVRNQTISLTDALYN
                     LTQKQLDYQKEMIDKRAIKLARFPTIKSLEEFDFEFQPSINKHDILQLKSFHFLESST
                     NICFLGNSGVGKTHLATAIGIEACIQGVKTRFILFNDLIEKLTRASQRGTLVRTLKIF
                     TNVPLLIIDEIGYTPISKTQADWFYQLMARRYERASTIITTNIPFSMWAKNFDNTTAS
                     AAILDRLIHHSKVFKITGNSYRLKNYHLDKETIG"
     gene            complement(2583..3361)
                     /locus_tag="IXH04_002536"
                     /pseudo
     CDS             complement(2583..3361)
                     /locus_tag="IXH04_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
BASE COUNT         1176 a          559 c          521 g         1105 t
ORIGIN      
        1 tagcttcttc acataaactt tttactttat taattactac tttgtcatat tcatttaaca
       61 cactattatt accattactt agacgctcag attctacgaa acaactcaaa tcagaaattt
      121 cccaaaaaaa ttttttgtta aaatccaatt tcctcaactc ctccaaacta cgtaaatgat
      181 atagtctcat aataaaataa aacctctcag ctaatgttgt tgaaattgtc cactaaacct
      241 aattgatatt gcttaggaga tacacctgcg tactttttga atactctcga aaagtgtgaa
      301 gaatctgaaa atcctaaacc taaagaaact tcactgatag tatggtttga tttcaacatt
      361 attttagccg tagaaatttt tcgcttagag atataatgta aatggcggct ataaaatgta
      421 ggaaaacggc ggaataaaaa tgtcgttttt cctacatttt tttctataca cttttcttaa
      481 ctcaatctga atggaggtta attgattgag aaaagatgta tacgaaggag ttcgattttt
      541 tatgattaac gatattaaac ctaatttcaa tgaattggga cgccagtata attgcgaccc
      601 acgtactgtc aaaaagtatt atctggaagg ctcagaaaac tctgtaaaga aagacaaaga
      661 taagcccaag cgtccctcca agttagatcc tttcaaggaa ataattgatc agaaattaga
      721 attaaattgt tccgctatgg ctattttcaa gtttttgaaa aaaattggtt atcagggtgg
      781 ctatactatt gttaaggatt attgtcgcaa taaaaaacac acagaaatta aaaaggcgac
      841 acttcgtgtt gaaacagttc ctggaacagc tgctcaagtc gattggaaag aagatatgac
      901 cttacacgat aaatttggta aatcttacac gttcaatctc tttctgtacg ttctacattt
      961 ttctaaattg aaattcatta cattaacttg ggatcgaaag caggacacct tattttcatg
     1021 cttgtatgaa gcatttaatc acaccggagg tgtcccaaaa gaaatatggt tcgacaatat
     1081 gaaaactgtg gttgatcggg cacgtacaca atatcgtaaa gtggttttta acactcgttt
     1141 ctatgagttc agtaaagatg ctggttttga agctattgcg tgccggagtt atcgacctca
     1201 aactaaaggc tccgtagaat ctttagctaa gctagttgag agactgaagc cttatgatta
     1261 tgaattctat gatgccgttg acttaattag tttagtgaat acgctctgtt acgatttgaa
     1321 ttatgaagaa atttctcaat ccacttatca gcgccctgtt gatctatggg aacgtgaaga
     1381 aaaaggaaaa ctacatccag ttaatgaagc gttgttaact ccctattttg aagacaatat
     1441 tgttcgtatt gtcaccaacg aatcaatggt caattttaga aaatcaaaat attctgtccc
     1501 tacaaaatat attggttgtg aagttgaaat agaaattgat ccaaccgaaa aatttatcga
     1561 aatattctac caaggagagg ttgtacaatc tcacgaatta actggtaagc gcctgaacta
     1621 taaagaaact gatatgtttg aaatattaaa atcagacgtg atgaaacaca aagatgatga
     1681 agaaatttat gagtatatac aacgaacatt gactcaatat gatgaacttt aaggagctga
     1741 aataataatg acaacaaatc accaaaaagt gttaaccaat ttagatgagc taggcttatt
     1801 aaaaatgaaa gagtattatc ctcattacct tgattccgtg aggaatcaga caatttcttt
     1861 gacagatgca ttgtataatc ttacgcaaaa gcaactggat tatcaaaaag aaatgataga
     1921 taagcgtgca attaaacttg cgcgcttccc aactattaaa tcattggaag aatttgattt
     1981 tgaatttcag cctagcatta ataagcatga cattctgcaa ctcaaaagtt ttcacttcct
     2041 agaaagtagt acgaatattt gttttcttgg caatagtggg gttggaaaaa ctcacttggc
     2101 tacagctata gggattgaag catgcattca aggagtcaaa acaaggttta ttctttttaa
     2161 cgatcttata gaaaaattga ctcgtgcgag tcagagggga acattagtta gaacactaaa
     2221 aatatttacc aatgttcctt tactgatcat agatgaaatt ggttatacgc cgattagtaa
     2281 gacacaagct gattggtttt atcaactgat ggcacgtcga tatgaacgcg cttctacaat
     2341 tattacaaca aacattccat tttctatgtg ggcgaaaaat tttgataata ctacagcttc
     2401 agcagcaatt ttagatcgat taatccatca ctctaaagtt tttaaaatta ctggtaattc
     2461 ttatcgattg aaaaattacc acttagataa agaaacgatc ggctaataac aggcatttat
     2521 taccgccgaa aaacaacatt tttaaaccgc cataaaacag catttttatt ccgccattga
     2581 caataattat aaatcgtaat atgcatttca ttcttaaagc tttctcttaa tttagtttca
     2641 cttacagaaa aataattagc tatttccttt acagtaattc ttttatagat atttaagccg
     2701 atgaattgaa cgacagaaga aattaaggga gaaagatgct ctatcttagt ttttccgatt
     2761 tccttagtat aataaacaat cccagaatcc ctcactttta agacctcagg taacgataca
     2821 gctaattcgt tttcttgtat caaagcatct ctactttgat acgcttctat tatatccatt
     2881 cctaatgtta tagacgtttg tgctaacttt tcaaaaacaa tgatagaata atttttttca
     2941 gaccgaatcg tatctccact aacacttgga attattccat tactcagatt aaaaaccatg
     3001 ttttctaaat ttcgaatatc tccattttta acatattcca gtattttatt ttcataaaga
     3061 aacagatata tttctggatc gtaattttgt gataacagat tatctatttt gatttgctcg
     3121 ataattttac ttagattttc tgtatattca attaacggtt tatggaatag atcttctatt
     3181 tttccagtaa aaaaataatg aactaaaatc agaatatctc gaatgtctcc taaagaatac
     3241 aaagtcaaat tttgcaatga attatagagt cgttctctat cagccattgc aacattcaaa
     3301 ttgttcattt ttagtttaaa aaaatcttta tcaatattat ttgctctaaa tggaccaaat
     3361 a
//
LOCUS       DACEXQ010000246         3326 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.246, whole genome shotgun sequence.
ACCESSION   DACEXQ010000246 DACEXQ010000000
VERSION     DACEXQ010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3326
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.246"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..75
                     /locus_tag="IXH04_002537"
     CDS             <1..75
                     /locus_tag="IXH04_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ1853095.1"
                     /translation="KVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(209..841)
                     /locus_tag="IXH04_002538"
     CDS             complement(209..841)
                     /locus_tag="IXH04_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853096.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(989..1531)
                     /locus_tag="IXH04_002539"
     CDS             complement(989..1531)
                     /locus_tag="IXH04_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAQ1853097.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            1666..2055
                     /locus_tag="IXH04_002540"
     CDS             1666..2055
                     /locus_tag="IXH04_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853098.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            2059..2823
                     /locus_tag="IXH04_002541"
     CDS             2059..2823
                     /locus_tag="IXH04_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ1853099.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            2952..>3326
                     /locus_tag="IXH04_002542"
     CDS             2952..>3326
                     /locus_tag="IXH04_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1853100.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWID"
BASE COUNT         1139 a          551 c          644 g          992 t
ORIGIN      
        1 aaagttagat ttttaaataa tcaatattat gattcagtca agtatggcat tttatgggaa
       61 gaactagctg attagattag gaagtccatt tgataatcca gaatcactaa gaaacgaatg
      121 gcgagtcatt aataatttat ccaatcttgg gttattcttt tagattgaag tgtttaaaac
      181 taaaaaagca tttatgaagt cgtaatagtc aggcttcaaa atgcttttta ttttgtttgt
      241 tttttctttg ccacataaat acacctagac tgacgatgaa gagcatatcc aacagatttc
      301 ctgcgacttg gatcgtcgaa gggttggtca gtggcattaa agcaaggaca aaaaaggaaa
      361 acaagctgta aataaaacag gtgacaggaa ctaatcgaaa gaagagcgga catttcaatt
      421 ggctaaaaat gagtgaaaaa ataaaataag taaaaatgga taaaatcacg actaaaccat
      481 tcatgataaa gaagaaaagc gtattgtgat taaacgaata aaaaagaatt gctaaaaaag
      541 aaaacgtttt aaatgcaatc aatacgcttg aagcttcgtt taacgtacca ggatgtcgat
      601 gaaaaaaata ggcaccgaaa taaacagaaa acagtaagcc gcaaagagaa gacaaaatca
      661 aaaaaaacgg attttcttcg gcttctgtga tatcagaatg atccaaaagc aaccagccta
      721 atgcactaga aggcgcatgt gaggtcttaa ggtatgaata acatagaaaa aataagatcg
      781 tcaaagttgc aaaaaaagag cctaaatgta aaaaattagt ggttctacgt tgaatttcca
      841 taatatctcc aaattaaatt gataagtata taaagtataa taacaaattg atgagtatat
      901 gtcgatgttt tttaatcaaa ataataaata aaaactgtct aacatttaaa atttaattta
      961 gatattagac agtttttaag tttgtgagtt attttccttg tcgttttctc acttttttag
     1021 tccagaaacc acctgaaata tattttggct cataagaaat gataaaagca cgctcttcga
     1081 tttctttgat caggcgatac agttcacgtt cgctttttct aggagaaagt atttcgagaa
     1141 ctagccgctc accttccagc cccattgcag aagtctgtgt cacaccataa ccgtgttctc
     1201 tcagtatacc cggcaaactt ttatcttctg cgctatttgc tggcaaaatc gttgtgacca
     1261 tgatgtatcc taatgccaat ttgtcttcaa tcaaaattcc gacactgata cctactgcgt
     1321 aacctaacgc atagacaaat aaattcagtg gattatctaa acgatctaaa acaagggata
     1381 agccgacgat ataaatcgtg atctcgacca tactgacaag tggagcaatc agtcgatagc
     1441 ctttcattgt caacatgaaa cgtaacgtgt tcagagtgat ataagcaaaa ttaatgagga
     1501 agatcatccc caacattttt ggatcaatca tttgtaaaac tcctttcatt tatataacaa
     1561 caatatcaaa aaaaatcgta agtgaagaaa aattgaaact ttatacatac aaaaaaatga
     1621 taaaatgatt ataatagaat tataaagata ggttaggtga ttaaaatgaa aacggtcaat
     1681 cgtgtgtttt gtgccagcca cgtgctaagt ctccttctca gtatatactt aggtgtctat
     1741 tcgattcaac agccaattga ggtagagaac attgtttttc agatgtcgct gacagatggt
     1801 ttaattttaa gcgttttttt ctttttgata ctgtttattg gtaatatttt cggtagcgtt
     1861 gccagttttt tgaatttttc tatttatcca ctcctttcgc tagcattagg agtggtggga
     1921 ctttgttctc tgtgcttatt tcccgagcca ttcagtcctg catttatatt atttggtaca
     1981 ctgaacctgt tccagacaac tgttggagca tggttgctat ggcgatcagg aaatttaatg
     2041 aaaataggtg agtaacaatt gaatccaatt atattgaaac gttctgtcca tcatattcca
     2101 gtgttagaag ttgttctgaa agaattaaga gaggaacgta ttcccgttgt catctattat
     2161 cacggatggc agacaagtaa ggagttagtt ttgacgcaag gaagaaaaat tgcgcagcag
     2221 ggcattcgtg tgatcctgcc agatgcgatg aatcatgggg agcgcaaaca gccggtttct
     2281 tctattccat cctatacatt ttgggatagt atctacggaa atctgtttga atttgacaca
     2341 cttatcaagc atttagaaaa aagaggtctt ctacatgatc agcttgcagt tggcggggtt
     2401 tcgatggggg gaatgacaac atgcgccttg ttggctaaac atcctcatat agtagcgggc
     2461 gtgtgtttga tgggttcccc tgcacctttg gaatatggaa aagaaatcgt aaggcgcgca
     2521 tcggaatata attatgtcgt accaaaagac tattttgatt tgatttcatg ggttaagagc
     2581 tatgatttgt cattgcagcc ggaaaagtta gctggacgtc ctctgttctt ttggcatgga
     2641 caagctgacg aaaaaatccc ttatatacaa acggaacgat ttgtagaaaa aaatcggcat
     2701 aagtcttacg gaaaagaaat tgtttttcgt tcctcaaaaa cacagcgaca tatggtacaa
     2761 ataccactaa tggaagaagc ttccactttt ttagcaaaaa atttgataaa aaagtataaa
     2821 taattgaaac ggaaaatcac tcatgacgat agaggatttt ccgtttttta ttttgggcta
     2881 taataaactc tgttaggaaa atttggtata attgatagta agaatgacaa catgttagaa
     2941 aggaagagag catgcccact ttagcagatg ttgcaaaacg tgccaatgtt tcaaaaatga
     3001 cggtctctcg agtcatcaat catccggaac aggtgactga cgaattgaaa gagttagttt
     3061 tctcagcaat ggctgaactg gattaccgtc caaatattgc tgcaaaagca cttgtcacga
     3121 atcggtcgca gatcatcaaa ttatttatac tggaagaaat cgacacaact gagccctatt
     3181 acatgaattt gctgatggga atcgctaaac ggatcggtaa agcgcattat tctctccaat
     3241 tagttacaaa tgatgccttc gacgtcggtt cttgtgatgg atacatcatc acaggtgtca
     3301 gaaaaaaaga cttcgattgg atcgac
//
LOCUS       DACEXQ010000247         3300 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.247, whole genome shotgun sequence.
ACCESSION   DACEXQ010000247 DACEXQ010000000
VERSION     DACEXQ010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3300
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.247"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            99..1040
                     /locus_tag="IXH04_002543"
     CDS             99..1040
                     /locus_tag="IXH04_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAQ1853101.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKTFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            1030..3201
                     /locus_tag="IXH04_002544"
                     /pseudo
     CDS             1030..3201
                     /locus_tag="IXH04_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         1021 a          652 c          607 g         1020 t
ORIGIN      
        1 gtgttatttc tggtatattt catcattgtg ctttttgctc ttgtattcaa caattcttta
       61 ggctggttct tgttggcctt ttttactctt tatgtatcat ggagcttgat ttcattaagc
      121 agctttctaa agaaactttc ggttacttgt acattgcagc cactcgttta tcgcaaaaaa
      181 agtatgcagg ttattttttc catagcaaaa aagagaactt tttctttacc tctccctcgt
      241 ttacaaatcg gcttcccagc aatctttgca gaaaaaaaca agagattttg attcttacaa
      301 ataaaccgca agaagtccaa ttgctttggc agccaaaaga aagaggatgc catgaaacat
      361 taactgtcat ttttacagcc tatgatagtc taggactttt cagaaaaagt gcaaaaagag
      421 agatctcttt tcctgtgact gttctgcctg ctttttgtaa gattcaagca gaaaaattac
      481 agagagttct ggaaaaaaaa cagcagctga acgcgtacca aagaggtctt gattttcggg
      541 aacatcgcgc ttatcgtcca ggtgattctt tcaaccgaat cgattggaaa ttgtctgctc
      601 atactcaaga gtggttatac cgtgaatacg attatcaaga agaagaatgt tctcctcttt
      661 tttgcttttg gggttcgccc agtccaaaat ttgaacctct gctagacttg tatttttctt
      721 tatggcattt gagaaaagaa aagcagccgg aacttctgat attaggcaac cgcgcctttc
      781 atggcaaaga ccccggtcat acctattttg cttctttgga agctggcgat gagaaaactt
      841 ttttcgcaca cttgaaaaaa tatgcaaaaa aacaaatcgt cctctttgtt cctgagaatt
      901 ctcctgaagt ctcagaagct attgaaacat tgtcaaaaga tcgaacggtt tatcttgttt
      961 atttcgtaga aaaacaactc atggtccaag aaaaagacaa gatatgttca ttgccaggag
     1021 gtgaatggat tgacgattaa acgggaaaaa tggctgcgtg gcgtttactc ctttgtgata
     1081 ctaagtgtag gtgctccgat atttgtagta gctaatcaat tgcaaaacag ctaccttctt
     1141 atcctaacta ttggtgtcct atgccttatt gtctgctgta ttcctaaaaa atgggtacgt
     1201 ttgctgcttt ctttgccagt aatgataggt tgtttgtttt tatattttcc ttcgaagaca
     1261 ttttccatat tatggatctt acagtttttt tcgtcagtta cagaggaatt cttccatcta
     1321 tcccaaggta aactttatta tttgccagaa aagacagctt tttttctcat aatgttgctg
     1381 atctatttat ttattacatt gaccgtggat tacgactatt ggtacgcacc ctttctttct
     1441 ttgatggctt acttcatgat gcttaccgtt tttcggaaaa aagatttact gtacgaaatg
     1501 attgcggtag taactgtttt gttcgtgtat ctggcagcaa ggcaattttt ctccatccgg
     1561 ttgttgtcga gaaacactcg attccagtca ttgactacag ttctgcttat ttgtttctta
     1621 acttttagcg ctatcttgcc tttatatctt ccgaaagtac accaatctct cgaggaaacg
     1681 agtgaaccga tccgagaata tcttaatgat gaagggcttt atgacacact ccatgagtat
     1741 caattaaccg gaagtgcaag aacaggcttc agtgagaatg atgaacagct tggtggacct
     1801 ttatatgata atggcgaagt tgtttttaca gcgaaccaaa aaggaaatgg ctattggaag
     1861 atcgaaaata aaaatcatta caccggttct ggatgaatag aagatcagga cgcacccatc
     1921 caaccaatcc aagaattccc tgttgacatc acgagctacc aaaccgttat agaagacaaa
     1981 gaaaacatta cattgaccct aaaaaaacaa gctttcttac cttatcctta tggaaatact
     2041 accttgactg atttttcatt agctagtgga caaacgcttt actatttgcc tgaaagccag
     2101 cgattcattc ctgtcccgaa tgaatcgaca cgttctgcaa ctttacaaac agaaaggtta
     2161 gtttttacga gagaagaatt aaaaagcagt cagttaaaag ctgatgattt agaaaatcag
     2221 cagacaaatc ttcaattgcc aaaaagtttg cctaaacgta ttgaggactt ggcggtcgaa
     2281 ttgaccaaaa atgaagcaac tttaattcgt aaagtcgaag ctgtctaaca gtatttaaaa
     2341 acaagcggaa atttccgcta ttccaaaacg gataccgggc atactccaaa agaacacgat
     2401 tatgtggatt atttcctctt tgattcaaaa gtcggctatt gtgacaactt ttcaacttct
     2461 atggtcgtcc ttcttcgaac acttggtatc ccagctcgct ggacaaaagg ttttacaaat
     2521 gggctcagaa caggcacaga taatcaaggg aatgaggaat acagcattct caatagtcat
     2581 gctcattctt ggccagaggt atactttcct ggatttggct ggcttccatt tgaaccaacc
     2641 ccttcatttg ccaatccaat gggttcacaa ccagttgctg aaacaaccga aaccgtccag
     2701 cctgctatcg aacaaccaaa tgaaagcgaa aatatggcag aaactatttc cagtgagaga
     2761 tctgaggtca ttccatcaga agaaacaaca ctcacaccag cttataaaat cgtcttattc
     2821 agccttggag tgtgtttatc cgtcttgttg ttctttctat tgttattcag aaaatctctg
     2881 cgctggacaa gttatcttat ctatcttcat ttttttgctt ctactaatac gattgcatat
     2941 caaaaacttc ttaacccttt ggaaacattg gtaccgcgca aacccgcaga atctttgcag
     3001 gaatatagcc tgcgaataga acaagaattc aagctagatc tgcgtttcat tcgattaact
     3061 actcaatatg aagcagaaat atatggacga caagcagatc cggtaaaatt cgataaaaaa
     3121 acctatttag aaataacgca tataataaga aaaacggtat acaaaaaaat tctgacaaaa
     3181 gggagccgct tcaaaaaata agataaaatt tctggagaat ctacttctcg aatgccgaga
     3241 accagctgat tgagtatcga ttactcgaaa aaaaggttgt gacacgtttt ttgtcacaac
//
LOCUS       DACEXQ010000248         3281 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.248, whole genome shotgun sequence.
ACCESSION   DACEXQ010000248 DACEXQ010000000
VERSION     DACEXQ010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.248"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            32..1156
                     /locus_tag="IXH04_002545"
     CDS             32..1156
                     /locus_tag="IXH04_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ1853102.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            1383..2300
                     /locus_tag="IXH04_002546"
     CDS             1383..2300
                     /locus_tag="IXH04_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1853103.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            2293..3126
                     /locus_tag="IXH04_002547"
     CDS             2293..3126
                     /locus_tag="IXH04_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1853104.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
BASE COUNT         1099 a          530 c          631 g         1021 t
ORIGIN      
        1 ataagaactt gaaaagaaag aagggataga catgaaaaaa agatacaaat tggtgatagc
       61 tttgctaaac attaccctat tattcagctt aagtgcatgt ggaaaaacta acgaagaagt
      121 aacagcaaac atcgcagatc caatgataaa aaaagccact acattagaag tatatgtcgc
      181 aaacggcaaa ctgacaaact atgttgaaaa tgctgcaaaa atctataacc gcaaaactgg
      241 agcaaatatt gacttaaaaa ttgtgaatgt agcttcggga acagcaacag tacaaatgat
      301 caccccaaaa ttggtaacac acgaagatat gccggatatt atctccatgg cagacacttc
      361 agcagcaggt gtgctggaaa aatttcaaga tgctttttat agtgctgatg aatatggatt
      421 ttacgacaaa tatggcgatc agttttatga tcaaaaattg aatattttgc gtcaacaaac
      481 gagtaatgaa acgattattc cttgggctag tgattttacc ccagcacttt gttattatca
      541 accaaaaaaa tttgctgaaa taggtgtaga ttttgaagaa atcagttctt gggaagaata
      601 tatccaggtg gcaaaagcat taaaagaaaa aactggtagt tttggaattg ccttgccaga
      661 atctggggac caagaattgt ttatcaacat gctagcccag cagaatcaat ctttgcttga
      721 tgaagaagga aatatccgtc tgaatactaa agaagcgaaa catgctgcaa agttaatcaa
      781 agaaatgatc gactcagata ttgtacattt ctacggtgca caagatgctg agaaagcatt
      841 tcaagaatct gcaatgtttg ttgctggcgg ctggtatgct acgaatatgt ctttgaattt
      901 tcctgatgca tctgacaaat ggcgcatggc tccactgatt cctttttcaa aagaaaatcc
      961 aggaaaaggt gcagtatcag gaggatcaag tttttatgtc cctaaagatg ccaaaaatcc
     1021 acaagtggca caacaatttc ttacatttat gctgacagat gaagaatgct tagccaacgc
     1081 actggagctg ggcgtggcta catctaactc attggcctat caaacagaag cagctgagaa
     1141 aaagtttgac tactaaggag gacaaaaata ctatgaacta ttgaataaaa tcaatgaaaa
     1201 tacatcaaat actgttttac cagccactta ttcagatgct acgtcttatt tgactaccgc
     1261 tgcatataac tattggcaaa caggtgattt tgaaaattca tatctaaaag aagcagaaaa
     1321 ttttgctcat aagtacggcg taaaattaac tggtgattaa aaaatattag gaggacttaa
     1381 cgatgatgca atcttcgtta ccaagcaaga caataaagaa cagcaagaaa aaagcaacag
     1441 atacgtctca taaaaaaagt gcttatggat tggtcacacc ttttgttatt ttctttattg
     1501 tgacgatttt gattcccatc attatctcgt ttgcttttag ttttatgaaa attggctact
     1561 catttgaatt tgttggattt gacaattatc tagctatctt ccgagatcca ttgtttatta
     1621 aatcttacac gaatgttttg attatcatgg caggatcaat tccaataacg atgctattag
     1681 ctatttactt tgctgtttta ttgaattcag cacagttaaa aggaaaagga ttttttagaa
     1741 ctgtctatta tattccaacc attacatcaa cggtggctgt agcttccgta ttcatgactt
     1801 ttttcaatcc gactgggtta ttcaatggat ttttgaatgc gctaggaatt aacggtgtac
     1861 agtggttaac aaatcctttt tggatacgaa tatcaatgat catcacgatg atttggctta
     1921 gtgtaggata taacactatt ttatttttag ctggtcttca agggatttct aaagagatat
     1981 acgaaagtgc tgaaatagat ggagcatcga aatttcggca attcttttta ttaacgatcc
     2041 ccatgctaaa gccaatcatt ttaatggcca cagttttagc cactatcaat ggcttgggta
     2101 atttcaatat cccaaatata ttctttggga cttccaatgg accagaaaat tcagctttgg
     2161 tcgtaggagt caatttatat aaaacaagtt ttgaaatggt tgattttgga aaagcctctg
     2221 ctattgcctg gacgatggtg tttgtatctg ttatattatc ggctattcaa tttaaattcg
     2281 gaggtgataa aaatgactaa acaaaagata caaagcagaa tttttacgta cgtcattatt
     2341 gtgtttggtg ccttaatatt tttgatgcca tatttatata tgattgtagc ttcaacgcaa
     2401 agtaatcaag aaattttaag tggcggaatc aatttcaagt ttggggatat gctattaaaa
     2461 aactggcaag ccatcatggc gagatataat tatcccttga ttatttggaa ttcgattgtt
     2521 attgctgtaa tcagtacagt tttagctaca gccagttcta caatggcggg ttatgcatta
     2581 gcaaagtatc gttttatggg gaataaactg ttgttcaata cagttatgct atctaggatg
     2641 gtgccttttt ttaccacatt gattccttta ttttacatta tgagtcgttt aggattatcg
     2701 aatacttatg caggtgtgat catcccttcg attgccagta caacttcagt gtttatgatg
     2761 cgacaatata gcagtcaatt tcctacagca ctgatggagg ctgccagaat cgatggtgcc
     2821 aatgagtggg tgatttttac gaaaatcgct gtaccgattt tatcacctac gattattact
     2881 agtggtttac ttatctttat gggaagttgg aatcaatatc tatttccatt agtcatgctt
     2941 tcagataaag ataaatatac ggttccctta attatcaaaa atatgacggt ttctagtaca
     3001 ggtgagccaa ttaattatgg cgcaattatg ctaattttag caacttcagt gatcccaatg
     3061 gtcttgattt atgtgtgggc gcagtctaaa tttaaggaga atgacattgg agctgcaaat
     3121 aagtaaaaaa aataacgaat gttaaccatc tagttcgttc taggagcaac tccacattca
     3181 taaaaattaa aagaatggcg ggatatccat gaatgaacag acgaaggaat ggtcaagaaa
     3241 aattttgaat gtagcttctg aaaagataga tgcggaatta g
//
LOCUS       DACEXQ010000249         3264 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.249, whole genome shotgun sequence.
ACCESSION   DACEXQ010000249 DACEXQ010000000
VERSION     DACEXQ010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.249"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(1..803)
                     /locus_tag="IXH04_002548"
                     /pseudo
     CDS             complement(1..803)
                     /locus_tag="IXH04_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            complement(838..2451)
                     /locus_tag="IXH04_002549"
     CDS             complement(838..2451)
                     /locus_tag="IXH04_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4368 domain-containing protein"
                     /protein_id="HAQ1853105.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFDRPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAHYGA
                     YAPLGYKKHPDIKGKLLVDDETKWIIEKIFSLAYQGYGSAKITKQLRAEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNMQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     GTNKTNKTPYSYYACSYYGQFGKGNCSMHYIRYDVLYQAVLERLQYWAKAVQQDEEKV
                     LNKIQKAGNAERIREKKKKASTLKKAENRQNEIDRLFAKMYEDRACEKITERNFVMLS
                     SKYQKEQIELEQQITSLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEATTNEDNERIQEIEIYYRFIGRVE"
     gene            complement(2563..2772)
                     /locus_tag="IXH04_002550"
     CDS             complement(2563..2772)
                     /locus_tag="IXH04_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
                     /protein_id="HAQ1853106.1"
                     /translation="MNCLERFYRKGVCVIDNAVKTIQKKNGKCGIGNRGKTKIVVKEH
                     FSEKGKTMEELLTDVMLEKAKQTIA"
     gene            2863..3051
                     /locus_tag="IXH04_002551"
     CDS             2863..3051
                     /locus_tag="IXH04_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853107.1"
                     /translation="MAVREYHYLTVQVMAIKEDKSFTRTQIGFRSLLQGKGRGVFVST
                     ASCLSNVLNSFIQLSKND"
BASE COUNT          889 a          725 c          545 g         1105 t
ORIGIN      
        1 tgagcaggaa caccgtgtgt gttttctttt gcaaaatatt caggaagtaa tccttcttct
       61 gctgctacgt gaagtgcttt tgaaggagag gttacccaag aagcaatttc tccgatgaca
      121 cctaaacaaa caagaatcgc aagaattttc gcaatccatg caagatttgg atcaatgtgt
      181 cttaatagga attctaacga ttgaatcacc cctgtattaa gagacaaacc atgttgaggt
      241 actgtagtag cgacggtcaa tccgccaaaa gtatctaaaa tgattgccag aataaccaat
      301 acaaacattg ctaatggata atccttttta gggttttcca tttcatttgc atgactggca
      361 gatgtttcta cgcccatata tgctaaaata aaggaaacaa aaacaaccag cgtaggcaat
      421 ttcgaaaaat caggaatcaa tgttttcata gacagtatcg tttgtagtgg agcgcctgaa
      481 gcgacataat ggatccctaa gccaaacaag actaatgctg gcaacaagat accagctaca
      541 aaaccgatcc gagcaatcaa tgaagtgtat ttggttcctt ttaactgtga gagtaaaata
      601 gcccagaaaa taatcaatag gattgccaat ttcagatatt tattgttttg tatagcacca
      661 atacccgttg cataggataa tgcaccagtt aagaaataca gcatggtcaa gtagccaact
      721 gttatctcaa accattggaa aaagattgcg gcaaaaccga atcgttttcc caatgtgcga
      781 ctgacccaag tatagacacc gccggtttcc cttagttaaa aatattactc ttgttgatta
      841 ctcaactctt ccaataaatc ggtaatatat ctcaatttcc tgtattcttt cgttatcctc
      901 attcgttgtt gcttcatgta tgaggatttt ttctatcatg gcatttaata atggtgctgt
      961 cagttccttt ggtacggaat attccttgat taactcaatc cacttttcag cacctatcat
     1021 atcctgttcc attttactta gttcttctct taggcttgtt atctgctgtt ccagttctat
     1081 ctgttctttt tggtacttgc tggacaacat cacaaaattt cgctctgtta tcttctcaca
     1141 ggctctatct tcatacattt tcgcaaataa acggtcaatc tcattttgtc tgttctcggc
     1201 tttcttcaat gtacttgcct ttttcttctt ttcccgtatt ctctctgcat tgccagcttt
     1261 ctgtatcttg ttcaatacct tttcttcgtc ctgctgtact gccttagccc aatactgcaa
     1321 gcgttccaat acggcttgat acagcacatc ataacgaatg tagtgcatag aacaattacc
     1381 tttgccaaac tgcccgtagt aactgcaagc ataataactg taaggcgttt tatttgtctt
     1441 attcgttcca aatctcatag accaaccaca atccgcacac ttgacaagcc ctgcaaaaat
     1501 cggcgttgcc ttttcctttg tctgtctgcg tcttgatttt atctgctcct gcacacgata
     1561 gaacacttcc ttgtcaataa tcggctcgtg agtgttttct actcgaaacc attcactttc
     1621 gggcttacgc actttctttt tgctcttgaa cgataccgta gactgcatat tgtgaacact
     1681 gtttccgata taaacctcac ttttcaatat cgccttgaca tgagcaatcg tccactcata
     1741 acgcttactt tcgggttttc cctcaaagat atgtgcaaaa gtaccgtatc ttgtaaaatt
     1801 tagccatgac gctgtcggaa ctttttctgc tcttaactgc tttgtgattt ttgcacttcc
     1861 gtaaccttga taggctaggg aaaagatttt ttcaataatc cattttgttt catcatcaac
     1921 caacagtttt cctttgatgt cagggtgttt cttatatcct aacggagcat aagcaccgta
     1981 atgagccccc tccgcaaact ttgtcttaaa tgctgatttg actttacggc ttgtatctct
     2041 tgccacccat tcattgatga tgttcttaaa tggtgcaatc tcactttcgc ctttttcgct
     2101 gtctacaccg tcatcaatcg ctatgtagcg gacattatga ctagggaaat acaattctgt
     2161 atattgtcct gtcataatat aatttctgcc aagacgggaa aggtctttcg ttacaacaca
     2221 gttgattttt cctgcctcta tatcctcaat cattctttga aaactcggtc tgtcaaaatt
     2281 tgttcccgac cagccgtcat caatatattc atcaatgaca ttcaaatgat gttcttttgc
     2341 atataaacgc aacatacttc tctgtgtggt aatgctggaa ctttcgccct gtaattcatc
     2401 atctcggctt aacctaagat aaagtgcagt attgtaaatc tgttgtttca ttgtcagtta
     2461 tcctccttta taactaaccc gtgtttacaa tacctgcttg caagtaaagt ataacacggg
     2521 tactttttgt cattcaatct atttcttaca actgcaccga ttttatgcga ttgtctgctt
     2581 tgctttttcc agcatgacat cagttagaag ttcttccatt gttttgccct tttcggaaaa
     2641 atgctctttt actacaatct ttgtctttcc tctgttgcca atgccacact tgccattttt
     2701 cttttgaatt gtttttacag cattatcaat aacacatacc ccctttctgt aaaatctttc
     2761 taagcaattc aacggctttt gtcatagccc acaggagttc cacctctgca tagttcttat
     2821 gtagccgtac ccattgcgtg cgacgctctt aatgctcaag ctgtggctgt acgggagtat
     2881 cattatctga cagtacaggt catggcgata aaagaggaca aatcttttac aagaacacaa
     2941 ataggttttc gctcgctctt gcaggggaaa ggtcgtggcg tatttgtttc aacggctagc
     3001 tgtctgtcaa acgtattgaa tagctttatt cagttgtcaa agaacgattg aaagaaaaaa
     3061 atcgtctttc ctatatatcg cgttggaaaa gaggagaaac gtaaccaaaa tccaaaactt
     3121 tttccattat tagtacaaat ggaacggttc attttggcaa tcaacggttg attttctcct
     3181 ttttcactac tatctgtaca gcaaacgtcc atttgctaag tttaaatgga aaaattttaa
     3241 cttcttttca tgcaccatat aggg
//
LOCUS       DACEXQ010000250         3256 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.250, whole genome shotgun sequence.
ACCESSION   DACEXQ010000250 DACEXQ010000000
VERSION     DACEXQ010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3256
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.250"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(441..1751)
                     /locus_tag="IXH04_002552"
     CDS             complement(441..1751)
                     /locus_tag="IXH04_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAQ1853108.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1763..3181)
                     /locus_tag="IXH04_002553"
     CDS             complement(1763..3181)
                     /locus_tag="IXH04_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1853109.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
BASE COUNT         1000 a          667 c          504 g         1085 t
ORIGIN      
        1 ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt caaaggtcta
       61 cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt atatcatatc
      121 agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg tttttcgaaa
      181 tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa cttttttgaa
      241 gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta gttatcttat
      301 cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt tcaatgaagc
      361 gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa atattattta
      421 agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc tgtgttgcca
      481 gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc gattcgcccg
      541 aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg cttttcagga
      601 aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag gcatgcctgt
      661 acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg tatttcttca
      721 gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt tctattgtat
      781 tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat attcagattt
      841 tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat tcgaatacca
      901 cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc agcactaact
      961 tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac ctcgattctt
     1021 gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat agcaatacaa
     1081 tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga atcaatatca
     1141 atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac taatacatcg
     1201 ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa atcacgaaca
     1261 acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc ataatccgca
     1321 atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg atcttttccc
     1381 caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc ttctataaga
     1441 atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc tacgccggct
     1501 actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag gactgcacct
     1561 tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa ataaatgcct
     1621 tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt ttgcagtatt
     1681 attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat aatccctttt
     1741 tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct tcaccaataa
     1801 atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg gatacaaata
     1861 agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact aaaactgatt
     1921 ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata aatccgccta
     1981 ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca tatactgttc
     2041 catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta atgaatgccc
     2101 agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga atcagatata
     2161 cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa gaagcaagta
     2221 acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta gcaaatggtg
     2281 ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct tgtttattat
     2341 ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga ttgatcacaa
     2401 tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa ccttttacca
     2461 actgagaagc agatactttt tcgtttttga caactcttac tctttcagta gtcaatttac
     2521 aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc caaaagaaat
     2581 gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa atcggaatga
     2641 agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg gacaacgagg
     2701 tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg atattgattg
     2761 ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc ttcaccgtca
     2821 ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa aatggatact
     2881 tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag acatcacata
     2941 atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag aataacgtac
     3001 ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag aaactattga
     3061 ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc ttatcggaaa
     3121 tcccaaattc ctttttttct ttttttattg tatttgctgt aacagcattt tgtgaaagca
     3181 tttgaattcc tcctacctct tctagtaaac gttttcaaaa ttataactct ttttttcttg
     3241 tttttatatt cacaat
//
LOCUS       DACEXQ010000251         3249 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.251, whole genome shotgun sequence.
ACCESSION   DACEXQ010000251 DACEXQ010000000
VERSION     DACEXQ010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.251"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..544
                     /locus_tag="IXH04_002554"
     CDS             <1..544
                     /locus_tag="IXH04_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF334 domain-containing protein"
                     /protein_id="HAQ1853110.1"
                     /translation="LEASTSNFNNKLDVFEEKTKHARLNFETTAKHYIKRLDEDNLKL
                     DFQQAIQDELSDTKDEIREVTKQAREETKEYKEILESKIKDHNKVVDKSNTALKVMTK
                     GVTNIFFVLIIFVLVMLVTGPIGHFFGIEHLYSFINGFIDDHESAWRYLMLILYAVPY
                     VFFAIILWLIALFFRVFNDL"
     gene            1220..1486
                     /locus_tag="IXH04_002555"
     CDS             1220..1486
                     /locus_tag="IXH04_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853111.1"
                     /translation="MAKITKNEQENLVENYTKKPAYSNSRLDAHFVCTSNPKLSFDAM
                     TIVGNLNKNSAKKLSDFMSLDPQIRLWDILQTKFKAKALQDPLC"
     gene            1544..3091
                     /locus_tag="IXH04_002556"
     CDS             1544..3091
                     /locus_tag="IXH04_002556"
                     /inference="COORDINATES: protein
                     motif:HMM:NF013677.1,HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ1853112.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLC
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGGPRGRMISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTREGTIA
                     HDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIASESSF
                     YRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRLYLII
                     DLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATFQVLL
                     EKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVDWYND
                     SHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQSVALN
                     PLKETES"
BASE COUNT         1191 a          547 c          643 g          868 t
ORIGIN      
        1 actagaggct tcgacctcga actttaataa taagttagat gtttttgaag aaaagactaa
       61 gcatgcaaga ttaaactttg aaacaactgc aaagcactat attaaacgac ttgatgaaga
      121 taatttgaaa ttagattttc aacaagctat tcaagatgaa ttaagcgata ctaaagacga
      181 aatacgtgaa gtgacgaaac aagcgagaga agaaacaaaa gagtataaag aaatcttaga
      241 aagtaaaata aaagaccata acaaagtggt tgataaatcg aatacagcgc taaaagtgat
      301 gacaaaaggc gttacaaata tattttttgt gctaattatt tttgtacttg taatgcttgt
      361 gacaggtccg ataggtcatt tctttggtat tgaacattta tatagcttta ttaatggttt
      421 tattgatgac cacgaaagtg catggcgata tttgatgttg atactttatg cagtacccta
      481 tgtattcttc gctatcattc tgtggctcat agcattgttt tttagagtat ttaatgacct
      541 ttaggtagat ttatatataa aaagggctaa atacgcaaaa taccccctta aaatgcaaaa
      601 taaatgcaaa taagggggta actccttatg aaaaaacaac taatagtatt ataacacgtt
      661 tttatgccga gaaaacttat tggttggaat gggctatgtg ttagctaact tgttagcgag
      721 ttggttggac ttgaattggg attaatccca agaaagtacc aactcaacaa cacataaagc
      781 cctgtaggtt ccgaccaata aggaaattgg aataaagcaa cgaaaggagt tgagtaaaat
      841 gagtgagaaa acgaagaaaa tcattcgagt atcattagct ttttcacaag tagtagtgca
      901 atcattgata gtttattata cttaccagca atataaatta tctaaagaga attaagtttt
      961 gattatgtaa taagttttgg tactaaaaaa gacttgatct aattagacca agtcttttgt
     1021 tagtgttata ttaataacaa aataaaaata agaagtcgct cactccccga ccaaagtttg
     1081 tgagcaaaat aaacagtcga tgtttgtttc ttaattgtat atcttgatat taaacgattt
     1141 aagattattc ttcaagatat atattcgcaa atgtaattgg aaaagcgact tcttaaacaa
     1201 aattaaggag tcgcttttta tggcaaaaat aacgaaaaat gagcaagaaa atttggtgga
     1261 aaattacaca aaaaaaccgg cgtactctaa tagccggtta gacgcacatt tcgtgtgcac
     1321 ttctaatccg aaattgagct ttgacgctat gacgatagtc ggaaacctta ataaaaacag
     1381 cgctaaaaag ctatccgatt ttatgagttt agatccacaa attagacttt gggatatact
     1441 tcaaacaaaa tttaaagcta aagcactaca agacccccta tgttaggcta gttgtcgtat
     1501 aggataataa gttataataa agactatacg gaggatgatt accatgacaa ataaaaacaa
     1561 tgaacgttac tctaccgaac tgaaagcgac agccatcaag aaaatgatgc cacccgaaaa
     1621 tagggggatt cgccaattaa gtcaaacttt gggcattcca gaagcgactc tttgttcttg
     1681 gcgtaaaaaa gctagaattc aagggaatac tacacccgga acgaaaaaaa atgtcgaaaa
     1741 atggtcaagt gaagataaat ttttaattgt gatggaatca ttctcaatga accaagtaga
     1801 acttgcggaa tattgccgaa agaagggctt atttaaagaa caaattgagg cttggaagaa
     1861 aacgtgctta tctgcgaatg aacaggaaga aaatcgaacg agagaacttg caactgaact
     1921 aaaagaagag aaaaaacaag cgcgtcaatt agagaaagaa ttacgtcata aggaaaaagc
     1981 attagcggaa gccgctgctc tcttattact aagaaaaaaa gcacacgcga tttggggggg
     2041 accaagagga agaatgattt catcttcaga tcgcaaacaa gctgttgaac ttattcaaga
     2101 agccaatcgc aatggggccc gtctcaccta tgcctgtaat gagctgaata ttagcgttcg
     2161 aacctacgaa cgatggacgc gtgaaggtac gatagcgcat gatcaacgcc ctttggcgaa
     2221 acgtcctgta cccaaaaata aactgacgga tcaagaacgc gaaaaaataa tagaaaccgt
     2281 ttcaaaaaaa gaatttatga atttgccacc tagccaaatt gtaccaaaac ttgcggattg
     2341 ctcgatttat attgcatcag agtctagctt ctatcgtgta ttgcgagaaa aaaatatgca
     2401 acatcaccgt ggccgaagtc aagtttcaca aaaacgcata ccgccaagtc accttgcgac
     2461 gaagccaaat gaagtttgga cttgggatat tacttggcta aaaggtccaa taaaaggact
     2521 cttttaccgg ttatatctca tcattgattt atttagccgt aaaatggtag gctgggagat
     2581 ttgggaatca gaagaagctc gtcatgccga aaaacttatt aaacagacgg tgcttaagga
     2641 acgtatccaa ggacagccgt tggttttaca ctccgataat ggcagcccta tgaaagccgc
     2701 gacttttcaa gtcttactcg aaaaattagg aattcaaagt tcttattcaa gaccacgagt
     2761 gagtaatgac aatccttatt cggaagcggc tttccgaacg ttaaaatatc gaccagaata
     2821 tcccaataac ggctttaaaa caatagatga tgctcgaaaa tggacgaaag agtttgtcga
     2881 ttggtataac gattctcatt tgcatagtgg gattcaattt gtgtctcctg cagcgtgtca
     2941 tgaaggacga catctcgcta ttttagagaa acgaaagcag gtttacgagg acgctaaaaa
     3001 gcgccatcct gaacgctggt caaaaggtgt tcgaaattgg actccacatc aatcggtggc
     3061 gttgaatcct ttaaaagaaa cagaaagtta acaggagcat aatctttcaa gattatcaaa
     3121 taaccctcgt cacccaatag gggaaaaagt tattcttccc ctattggata acgaggccaa
     3181 ccgaccatag ggaggttctg ctttttgata ggtggaaaat aaaaatacga caactatatt
     3241 gacaaacac
//
LOCUS       DACEXQ010000252         3232 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.252, whole genome shotgun sequence.
ACCESSION   DACEXQ010000252 DACEXQ010000000
VERSION     DACEXQ010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.252"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            49..1437
                     /locus_tag="IXH04_002557"
     CDS             49..1437
                     /locus_tag="IXH04_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HAQ1853113.1"
                     /translation="MYKERDDLNYTLIINKDGNYSWRPLVLLHPILYVDLVNCLTKED
                     HWEELKKRFSEFKKDKNINCYSIPVEASKGSKRTDLGETILNWWEYFEQKSLAQNIKY
                     QYCMFTDIADCYPSIYTHSITWAMYGKEKVKNDENKCSGSLGDKIDKKISGMQHNQTN
                     GIPQGSVLMDFVAEIVLGYVDLRLSKELKKLENLKDYKVLRYRDDYRIFSNNRNDLEQ
                     IMKVLSEVLYDLNFKLNSSKTKLSDDIIIDSIKKDKLYWEEIKASIRMNATNLDGDRE
                     IRYSINLQKHLLEIKKLADKHPNSGQLKKALSEFNKERIIKITSKPNDLLPLIGILVD
                     IMINNPGSLPQCVLIIGQLLNFENESKAREIVEMIINKYALKPNTEFMMIWFQRLTTI
                     FGEMFDSVLSSEFVSKIKDPVSNQMFSVDWLNEDAKKSFYEGTIKDEEKYIKMSKKIY
                     ITEFDDFNDYPF"
     gene            1775..>3232
                     /locus_tag="IXH04_002558"
     CDS             1775..>3232
                     /locus_tag="IXH04_002558"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAQ1853114.1"
                     /translation="MTEQIEKNSKSLYQALWNSADILRSKMDANEYKSYLLGLVFYKY
                     LSDNMLRYVSVLLEEETDDLNKAQDLYVEAFKDADIKDDLLYELKDEFSYTIAPALTF
                     TAQVAAIHDGSFQLEDLVQGFRDIEQSSENFENLFEDIDLYSKKLGSTPQKQNKTIAD
                     VMKELEGLNMAGHAGDVLGDAYEYLIGQFASESGKKAGEFYTPQPVAKLMTQIVLQGK
                     EDKKGFSVYDATMGSGSLLLNAKKYSHQPGTISYFGQELNTSTYNLARMMARMNMILH
                     GVPIANQHLHNADTLDQDWPTEEPTNFDGVLMNPPYSAKWSADKGFLDDPRFSAYGVL
                     APKSKADFAFLLHGYYHLKDTGVMAIVLPHGVLFRGGAEGKIRKALLENGAIDTVIGL
                     PANIFFNTSIPTTVIILKKDRAKKDVLFIDASQDFEKIKTQNTLRDYHIDAILEAYKT
                     RTIKDKYAYVAEFDEIVENDYNLNIPRYVDTFEEEE"
BASE COUNT         1187 a          507 c          588 g          950 t
ORIGIN      
        1 tcaaaaaaaa accaaccgaa agatataaaa gatattgtag atacacgaat gtataaagaa
       61 cgtgatgact taaactatac gcttattatc aataaagatg gaaactattc atggcgtcct
      121 ctcgttctac tacatcctat tctatatgta gatttggtta attgtctcac taaagaagac
      181 cattgggagg agttaaaaaa gaggtttagt gaatttaaaa aagataaaaa tattaactgt
      241 tattctatac ctgttgaagc ttccaaaggt tcaaaaagga cagatcttgg tgaaacaata
      301 ctaaactggt gggaatattt tgaacaaaaa agcttagctc agaatattaa ataccaatat
      361 tgcatgttta ctgacatcgc tgattgttat ccatcaattt atactcactc tataacatgg
      421 gccatgtatg gtaaagaaaa agttaaaaat gatgaaaata agtgttcggg ctcacttggc
      481 gataaaatag ataaaaaaat atctggaatg caacacaatc aaacaaatgg tatccctcag
      541 ggttccgttc ttatggattt tgtagcagag atcgttctag ggtatgttga tttgagatta
      601 tcgaaagaat taaaaaaatt agaaaattta aaagattaca aagtactcag atatagagat
      661 gattatagaa tattttcaaa taatcgtaat gatttggaac aaattatgaa agtgttatca
      721 gaagtcttat atgatttaaa ttttaagtta aattctagta aaacaaaatt atcagatgat
      781 attatcatag attctatcaa aaaagataag ctctattggg aagaaattaa ggcaagtata
      841 agaatgaatg ccacaaactt agatggtgat agagaaattc gttatagtat aaatttacaa
      901 aaacatttat tggaaataaa aaagttagca gataagcatc ctaattcagg ccaattgaaa
      961 aaagcacttt ctgagtttaa taaagaacgc attattaaaa tcacttcaaa gccgaacgat
     1021 cttttaccac ttattggtat cctggtagat atcatgataa ataacccggg ttctttgccg
     1081 cagtgtgtct taattatagg gcagttatta aatttcgaaa atgagagtaa ggctagagaa
     1141 atagttgaaa tgattataaa caaatatgct ttaaagccaa atactgaatt tatgatgata
     1201 tggtttcaaa gattgaccac catttttgga gaaatgtttg atagtgtttt atcatccgaa
     1261 tttgtatcta aaatcaaaga tcctgtctct aatcagatgt tttctgtaga ctggctaaat
     1321 gaagatgcta agaaatcttt ttatgaaggg accattaaag acgaagagaa atatattaaa
     1381 atgagtaaga aaatatatat tactgaattt gatgatttta atgattatcc tttttaagtg
     1441 aaaatttaga atgaatataa aaaacattac aatctaattt ttttaaaaaa atgttatatc
     1501 ggaagtaatc tcttgatttc tatactataa gataagaaaa ccactattta acaaggttat
     1561 tcgaacctta ttttaagtta cttattattt aatgtatcga aaaaggtgaa ataggtttgt
     1621 tataataaga attaagtaag acatgaattg tctaagcaaa tgtagcttat ccatttcatt
     1681 aaaatacata tggtgaaaaa acaagttcaa atagtaacta attttttaaa aaactgattt
     1741 ataaaaaata caacacgaat gaggaaaata accaatgaca gaacaaatcg aaaaaaattc
     1801 aaaaagctta tatcaggcac tgtggaattc cgcagatatc ctgcgttcaa aaatggacgc
     1861 taatgaatac aagagctatt tactaggctt ggtcttttat aaatacttat ccgacaacat
     1921 gctgcggtat gtctccgttc tattggagga agaaacggac gacctaaata aagcacaaga
     1981 cctttacgtt gaagcattta aagatgcaga tataaaggat gatctcttgt atgaactaaa
     2041 agatgagttt tcatatacca tcgcacccgc attgaccttt accgcgcaag tggcagccat
     2101 tcatgatgga tcttttcaat tagaagacct ggtacaaggt ttccgtgaca ttgaacaatc
     2161 cagcgaaaac tttgagaact tatttgaaga cattgatctg tattctaaaa aactggggtc
     2221 aaccccacaa aaacaaaata aaaccatcgc agatgttatg aaagaattag aagggttaaa
     2281 catggcgggg catgctggcg atgtcttagg cgatgcctat gagtacttaa ttggccaatt
     2341 cgcatctgaa tccggtaaaa aagccggtga attctatacc ccacaaccgg tcgcaaaatt
     2401 gatgacacaa attgttttac aaggaaaaga agataaaaaa ggcttttctg tttacgacgc
     2461 gaccatgggt tcgggctcgt tacttttgaa cgctaaaaaa tattcccatc aaccgggaac
     2521 catttcttat ttcggacaag agttgaatac gtctacctat aatttggcgc gtatgatggc
     2581 gcgtatgaac atgattttgc atggggttcc cattgccaac caacacttgc ataatgcgga
     2641 taccttggat caagactggc caacagaaga gccaacgaac ttcgatgggg tgctcatgaa
     2701 cccaccgtat tctgcaaaat ggtcggctga taaaggcttc ttagatgacc cacgcttctc
     2761 tgcctatggc gtattagccc ctaaatcaaa agcggacttt gctttcttat tacatggcta
     2821 ctatcatttg aaagatacgg gcgtcatggc cattgttttg ccacatgggg tgctctttag
     2881 agggggcgca gaagggaaga tacgcaaagc cttgttagaa aatggtgcca ttgacaccgt
     2941 cattggctta cctgctaaca tcttctttaa tacgtccatt ccaacgaccg taattatctt
     3001 gaagaaagac cgtgcgaaaa aagatgtgct ctttattgat gcgtcacaag attttgaaaa
     3061 aataaaaacg caaaatacgc tacgagatta ccatattgac gctattttag aggcctataa
     3121 aacccgtaca attaaagaca agtatgctta tgtcgcagaa tttgatgaaa ttgtggagaa
     3181 tgattacaac ttaaacattc cccgttacgt ggataccttt gaagaagaag aa
//
LOCUS       DACEXQ010000253         3172 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.253, whole genome shotgun sequence.
ACCESSION   DACEXQ010000253 DACEXQ010000000
VERSION     DACEXQ010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.253"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            111..800
                     /locus_tag="IXH04_002559"
     CDS             111..800
                     /locus_tag="IXH04_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAQ1853115.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            814..2316
                     /locus_tag="IXH04_002560"
     CDS             814..2316
                     /locus_tag="IXH04_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1853116.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            2329..2805
                     /locus_tag="IXH04_002561"
     CDS             2329..2805
                     /locus_tag="IXH04_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ1853117.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(2902..3012)
                     /locus_tag="IXH04_002562"
                     /pseudo
     CDS             complement(2902..3012)
                     /locus_tag="IXH04_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1046 a          425 c          618 g         1083 t
ORIGIN      
        1 aaaaaaactc ctaaatactc catttttatc tttactttta tgaaagcgga tgctatattg
       61 tttttggagt tgttatccaa aaataataaa aaattggagt gtttaggata atggataaaa
      121 agaggttttt agatcttatt tcaaaaggtt tgattgtttc ctgtcaagca ttacctggtg
      181 aaccatttta caaagaggaa gaaagtttaa tgccttattt tgctttggct gctcaaaggg
      241 ctggagcagt tggtatacgt gcaaattctg ttcgagatat tattgaaatt agaaagtgtg
      301 tatcattacc aatgattgga attattaaaa gagattatcc accagaatct ccattcatta
      361 ctgccactat gagagaagta gatgaattag tagaaaccgg tgttgaggta atcgctcttg
      421 attgcactct tagaaatcga catgatggac tatctataaa ttcttttatt actaaaataa
      481 aagaaaaata tcctcatcag ttatttatgg cagatatttc tacatttgaa gaaggcaaaa
      541 atgcatataa agcaggaata gattttgtag gaactacttt aagtggatat actgaggaaa
      601 gtaaaaaaca agatgggcca gatatagatt tgattaaaaa attggtagaa gaagggattt
      661 gtgtaattgc tgagggaagg attcatactc cagaacaagc aaaagagata aaaaaaatag
      721 gagttgcagg tattgttgtg ggtggtgcta ttactcgacc acaggaaatt gcatcaagat
      781 ttgttaaagc attaagttag ggggagaaat aaaatgttta aaaaattttc acaattagga
      841 cgcgctttca tgttaccaat tgctatatta ccagtagccg gattactatt gggactggga
      901 ggagcgttaa caaatgagag tgctataaat gcttatccat ttttagatca accgtggttg
      961 catacaattt taagtattat gagttatgct ggaaatgcag tttttgcaaa cctagcacta
     1021 atttttgcaa ttggtattgc tgtaggactt gcaaatggag ataaaggaac agctggttta
     1081 gctggtggag tagcttatct agtatatact gcaactatta gtgggtttct tgaacttttt
     1141 tcagcaaaag atgcaacact agatacaggt gtagtgggat caattgttgt tggtggtact
     1201 gttgcatttt tacacaatcg ttatagaaaa attgaattac cacaattctt aggttttttt
     1261 ggaggatcaa gatttattcc gattgtttct agtttagctg caatcattat tggttctttc
     1321 ttttatttaa tttggccacc aattcaaaat ggtttagtag ttgctggtga acaaattgcc
     1381 caaatgggta gtttaggaac ttttttatat ggttttttat tgcgtttaac aggtgctgtt
     1441 ggactacacc acaccattta ccctttgttc tggtatacat ctttaggtgg taccgaaact
     1501 gtagctggat caacaattgc tggagcccaa aatattttct ttgcccaact agctgatcca
     1561 aaccacactg gtctgtttac ttatggtact agattttttg caggaagatt tgcaacaatg
     1621 atgtttggat taccagcagc atgttatgca atgtatcgtg caattcctaa gaaaaatcgt
     1681 aaaaagaatg gtgggcttta ttttagcggg gcccttacat catttttaac tggaattact
     1741 gaaccagttg aatatatgtt tttatttgtt gctccttggc tttatgtgat tcatgcattc
     1801 ttagatggat tatcttttta ttttgctgat atcttaaata ttcgtgtagg gaattccttt
     1861 tcaggtggtt tgatcgactt tttattattc ggtgtacttc aagggaatga caaaactaat
     1921 tggttaaaat taattccttt tggtattgca tgggcattta tatatttctt tgtttttaca
     1981 ttctttatca aaaaatttaa ggtagctatt cctggaatgg aagaagaatc agaagttcat
     2041 tctgaaaaag taataaacaa aaatacatca ttacatgaag agtctcttat cattattgaa
     2101 gctcttggtg gaaaagaaaa tattgagtct gttgaagctt gtgcaactcg tttgcgtgtt
     2161 gctgttacga atggggagat tattcaaaaa caaacaataa aagacactgg tgcaactgct
     2221 gtcttcgaag tgaagggtgg catccaagct gtttttggag ggaaagctga cttattaagt
     2281 caagaaatca atcaaatatt aggaaaagat aattaggagg tttttaatat gtttgaaatt
     2341 ttcaaaaaga aaaaacaaga aactttttat gcaccttgtt ccggaaaaat catttccatc
     2401 acagaagtaa atgatcctgt cttttcacaa aaattactag gtgatggttt tgccgtcata
     2461 cctgatggtt caaaaattta tgctccattg gctggtcgag taacaaatat atttcctact
     2521 aaacatgcta ttaatataaa aagcaactca ggcattgatt atttgattca tattggaatt
     2581 gatacagttg aattacaagg agagcctttt tcgatttgtg ttcaagaaaa tgatgaggtt
     2641 actcctgaaa cctcgttggt tgatgtggat tttgacaaaa taataaaatc tggtaaagat
     2701 acttctgtta tagttgtttt tccggagaaa aatcagttca atgatttaat aatagataat
     2761 ggtcgaattg ctcatgggaa ttcatgtgga aaaatcactt tttaaaaaag tgtatataaa
     2821 aagctataaa ataagctaaa taggatattt gtcaaatgaa gttggtgaac agaaagaact
     2881 gttttctttc tgttcatttt tttattttat tagtaatgat tccccatgcc aaatcatgat
     2941 ccgtaattta aagttataaa agcttcgaaa tccattggca tttcgttgga ataccttgat
     3001 atgattattc aaatataaaa tatagagaga agagagagag ggaagatatt tataaagggc
     3061 aaagttatgt ttactcaact taggaaatag aaatagatca atttatattc tcttgtcaat
     3121 cgaatatgaa ccttagcact atataccaaa tatagtaaaa tgctgaaaga tg
//
LOCUS       DACEXQ010000254         3143 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.254, whole genome shotgun sequence.
ACCESSION   DACEXQ010000254 DACEXQ010000000
VERSION     DACEXQ010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.254"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            290..1135
                     /locus_tag="IXH04_002563"
     CDS             290..1135
                     /locus_tag="IXH04_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1853118.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1416..2636
                     /locus_tag="IXH04_002564"
     CDS             1416..2636
                     /locus_tag="IXH04_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAQ1853119.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2633..>3143
                     /locus_tag="IXH04_002565"
     CDS             2633..>3143
                     /locus_tag="IXH04_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1853120.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQ"
BASE COUNT         1154 a          452 c          582 g          955 t
ORIGIN      
        1 tttaaatctt ttgctaagtt gaactaatag agtcttttat gaatatcgaa gttagttttt
       61 cacctaactt cgttttttat agttggttat tataaaatag taatatagct gatttggatt
      121 caattcatat ttatttataa atcaaaaata cagagaatag tatataaatg tcctttccta
      181 ttcttaaaat gatgtttgta cttacttaaa ttaggtataa ttagtatatg ttttgtgaga
      241 aatttagact tacaataagc agtgaattta taatcaacgt ataggagaca tggatgaaat
      301 gaaactaatt ggtatagatt tagacggtac tcttttgaat tctgaccaga gcattagtca
      361 aaaaaatgca agaacaatga aacatttacc ggaagactgt tttccattca tttgttctgg
      421 aagagaagta gaagacatta ataatattct caaaaaatct ggtttaagga taccagcagt
      481 agggttaaat ggttctgttg gatttgatgg agaaaaaaag atatttgagt cttccttcga
      541 taaaaatgat gtaaaaaaaa ttgattctgt cctatcgaaa tttccaacca aaatatatac
      601 aaatctcgga agttatgaat ctcagaatta taaaaataaa atgaaaaaaa tttttcacga
      661 ggtagggagt gaattttcta tcaaagagct aaactatgag cttgagtatg aaaaatctat
      721 ttgttcaatc ccttataata atattgagga aattatagaa aaggatgcta tcagcattta
      781 caaatttttt atttttattc ctaatcgtca attgaaagct aatatattta accatttgaa
      841 aaagatgcta aatgtgtctg ttactgaatc ggcacctgta aatattgaaa tcgtacctca
      901 aaatgtatct aaaggtgtgg tgtttgatcg tttggaatca atctacaatt taaagaaacc
      961 tttgagaatc gcgatcggag atagtttaaa tgacttatct atgtttgaaa gtgcagatat
     1021 tagttttgca atgggaaata gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt
     1081 ttcaaatgat aaagacggag tatctcaagc atttgaaaga attcttacca tataaactaa
     1141 ttaaggtagt atagaacata gaattctaaa tacggaaaaa tggagcataa attccagtac
     1201 aaatagtact tcttcagtgt caaacgaagc gaatataaca tagatattta ggattgtgac
     1261 aaaaatcatg tcacaatctt tttttcgaat aaacttattt tttgaagtcg gccatttatc
     1321 gtaatttctt tgcataatac gaacaaattt tcggttattg atgaaaaaaa acattgaaaa
     1381 tggtatgata aaagaaaaaa aggaggaaat ttttaatggt gaaatttatc catgcggcag
     1441 atttacatat ggatcggtca tttgaaggac tgacaactct tgataaaact gtacaggaaa
     1501 aactattgaa gacgaatctg accgttttat caaatatcat tgaacaggca ataataaata
     1561 acgttgattt tgttttactt gcaggagata attttcatca aaatcgtcct tcattaaaaa
     1621 tacagaaaca tttttcagaa cagatgaaac ggttagaaaa gaaccatata cctgtattta
     1681 taatattcgg aaatcatgat ttctatcaaa aagagcggta ttggttctcc tttcctaaaa
     1741 atgtccattt atttacatcc gaaacggtag agacaaaaaa aataacgata aaaagtggag
     1801 aaactgtctc tctcagtgga tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga
     1861 tggaatttcc ttctagagaa ctcacagatt accatattgg gttgtaccac ggggaacctg
     1921 gcgtctctca aaaaggaaac tatgctcctt tccaaccctc aaagatgcaa gaaaagggat
     1981 acgattattg ggctttaggg catatccatg tgccaacggt cttaaatgaa aagcaaacaa
     2041 ttgtttatcc aggtgcgcct caaggtcata ctaaaaagga acaagcttct acatctattc
     2101 tactagttga attaagtaaa ggaagttgcc aaatacatcc gattaaagta gcagaggtct
     2161 attggaagac aaaggaaata tcattgagga aagcaagaac gacaaaagag gtcattgttc
     2221 acattcgtca gcagctttca aaagtagagg atggatttag tttaattgag ataaaattaa
     2281 aagattatga ccatctcaat acagatgttt tggaacggat acaatcagga gagctattag
     2341 actatttgtt agaggaattc tcagatagga taaatgattt ctttatttgg cgtatttctc
     2401 tcatagagaa tactttttat acgaagaccc cacttgctgc ttctgctaaa ttgatggaac
     2461 agttatttca atactatcag acacctcaag atttccaaca aattttaaat gaagtatata
     2521 gtcatcaaga agcggctaga atattgagcg aactgccgga atatcaagaa gaaacacttg
     2581 aaaaagccaa gcagctgctt aatcaagatt ttttatttga ggaggatcag gaatgaagat
     2641 tttaaggatc gagattgctg cttttggaaa atggcgtcaa aaaagttttg acttttattc
     2701 aggcaatcag ctgatctatg ggggaaacga agcaggaaaa tcaacgattt atcaatttat
     2761 tcaagcaata ttatttggtt tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa
     2821 agacggctca gcctatggcg gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc
     2881 tgtcgagcgc tacaagcagc aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt
     2941 gggaagtgat gagttactag aaaaactgat cgcacctttg acgaaagagt tattccagca
     3001 ggtatttact ttccagcaag agcagttgat ggaactagat catttgagag aaaaagagct
     3061 acatgatgcg ctgatttctt taggcatcac gggcagttct caagtattcc aaaagcggca
     3121 agaatattat cagaaagcac aac
//
LOCUS       DACEXQ010000255         3131 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.255, whole genome shotgun sequence.
ACCESSION   DACEXQ010000255 DACEXQ010000000
VERSION     DACEXQ010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.255"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            172..1476
                     /locus_tag="IXH04_002566"
     CDS             172..1476
                     /locus_tag="IXH04_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1853121.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEDTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            1572..2441
                     /locus_tag="IXH04_002567"
     CDS             1572..2441
                     /locus_tag="IXH04_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1853122.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(2553..2873)
                     /locus_tag="IXH04_002568"
     CDS             complement(2553..2873)
                     /locus_tag="IXH04_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAQ1853123.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
BASE COUNT         1043 a          596 c          622 g          870 t
ORIGIN      
        1 tgaagatgaa aaattgaaaa aaagacaaga attacttgaa gaagtgtata cggaacgttt
       61 tggaacaaaa gaagagagag aaacggtccg tttttattct gtctccgaag agaaaaatct
      121 agatacgaca ttcattgcag atttgtataa agaaaatggg gtggaactga aatgatctca
      181 atcacccata caggtaattt attcttagat acttgtttgt ctattatgta tttttttctc
      241 gtttcttatc cgattttagg cggatttgta tggtttattg gtgtctggtg ttatgtgttt
      301 ttatacaaac ataagcaaaa agaatgggta gatgttccct tgagtgtcga accgttcatc
      361 acgatcatgg tgccagccca taatgaagaa attgtgatcg aagatacaat cgagtatttg
      421 atgacgaaat tgaattatca caactatgag gtattagtga cagacgatgg ttcaacggac
      481 caaacaccag aaatattagc tagactgatg aaaaaatacg ctaatcttcg tgtggttcgg
      541 atcgaaaaaa ataaaggaaa agcccacgcg tttaatatcg gacttgcctt tgcaaaaggg
      601 aaattgattt taagcaatga tgcagacacg gtaccggagc cagatgcact gattaggtat
      661 gtgaattatt tcattcgccc tggtgcaaga catattgctg cggtcactgc aaatatggat
      721 gtccaaaatc gtaccaaatt gattgcaaaa tctcaaacgg ttgaattctc aagtatcgtg
      781 ggcatcatta aacgaacaca gtcagctgtt ttcggtggtc tttatgcgta cagtggagca
      841 aatacgatgt atcgaaaaga agccttgatc gatgtgggcg gattcagaca agatcgagcg
      901 acagaagaca tcagtattgc ttgggatcat cagttaaatg actggttatc tgtttttgct
      961 cctgagatta tctttttcat ggaagtacct gtgacgttga agatgctata tcgacacaga
     1021 aaacgttggg caaaaggtgg aacagaagtc tggctgacga atttcaaaaa agtgatgctc
     1081 catcctttta aacatattgg aagaacgatc atcttcattg atcaaacatt gagtattgtc
     1141 tggtcaatat tcttttgtat ttccgtagtt ctttttgctg gattaatcgg gcattatgta
     1201 tatcaaggaa actacgaaca gatttatatt acttttacat tcagttttgt gttcatctgc
     1261 tttgaaatgg tcgcaggatt tttccagttg ctagcttcgc tgattgtaga tgatcgacga
     1321 agaaaattga aataccttct tttcgcacca ctatatatgc ttttgttctg gatagtgaac
     1381 gcgataacga tcgtgacgac tttcattcca gcagtcaaaa cgattttagg ttatggaagc
     1441 gggacatgga aaagcccgga acgtacaaaa aaataaaaga tgcaccgttt tttagacaaa
     1501 cagcctgttg tgtttatggt tcttccaaac atttttgctt ataataagaa attagttaaa
     1561 aagtagtaag gatgggaaaa gtgatgaaaa aaagaatgaa gccgttaatc tggccaattg
     1621 ctgtttccag tattttctta ttgggacaga cagcttttgc ggaaacttct gaacctgctc
     1681 aaacagaacc ttcaacaacc ataagctctt ctggtgctga aaacggtgcc tcggaaacag
     1741 aaatgacttc caatgtaccg acagataatt caggagaaac gatttcatct tcagaagata
     1801 catcaataaa tagttctacc tcttctgacc aaatcgatca tttgcaagac ttggaaaata
     1861 aaagaaaaga actcgaagaa gcattagaag ctgcaaaaaa caaattgaaa gaagaccaag
     1921 cagcaatcga tcaagattta gctgatctta aaaataaagt gggaaatgac cgtgcacaac
     1981 ttgagaaaaa gcttgccgaa gcgaaagcaa aagcggaagc tgtccaaaca gatcttgacg
     2041 ggaagatcca acaagcaaaa gaattcaatc agcaattaaa tcaggttgct caaacagctt
     2101 ctggaaagct aagtcaaact caagcggata tcacagcggc cagtcaagca atccacggaa
     2161 agatcagcga agcacaaaca gttgctaatg attttgaagc aaaaaaagct gacttagcag
     2221 ctagtttcca acgaataaaa gatattttgc attcggcagg agaaaaatca gtgacctctg
     2281 gaagcacgac agaaacagtt cagcaaacga cagagccatc caaatcaaca agtcagactt
     2341 tgccaaaaac aaatgaaaaa acagcttctg tcagttattc cttagcaggg gcgggtgtgt
     2401 tagtcgcagc agcttacgga atcatccgca aaaagaaata aaaaagagag gatgggaagg
     2461 tagagtacct cgaagttaga ctgaaaaatc taattcttga gtgccactac atgtcccatc
     2521 tctctttagt tattaaaaca gatgtcccct acttatctcg tggagacagc tgttccacta
     2581 catgtgacca tcagcatgcc attgtcgctg cctaatactt catagtccat ttttacgcca
     2641 atgatcgcat cagctcctaa tgcttttgca cgttcggcca tttcggcaag tgcttcttct
     2701 cttgcaccca ttagttcgtt ttcatagctt tttgagcggc cgccaaagat atttcgcaac
     2761 cccgcctcca agtctttaaa tgcatggatc ccagtaatga cctcaccaaa aacgatccct
     2821 ttataagcta ctacggcttt tccctctaca ctgttcgttg ttgttgtaat cattcgatca
     2881 ctcctctctt ttaattaaag tataacaaac ttcaagaagc actacacata ttttcccaaa
     2941 attagaaatc gccgattgta aaattaagct agacaactaa aaaagtcatt tgtgctacac
     3001 tcaaaatagt tccgtcgaaa gaattataag gagtgagtac aaatgaccta tacccatctt
     3061 acaccaactg aactcgtaat gatagaagct tatttcaatc aatcacactc tgtttcaaaa
     3121 gtagcccatc t
//
LOCUS       DACEXQ010000256         3126 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.256, whole genome shotgun sequence.
ACCESSION   DACEXQ010000256 DACEXQ010000000
VERSION     DACEXQ010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.256"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            169..423
                     /locus_tag="IXH04_002569"
     CDS             169..423
                     /locus_tag="IXH04_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853124.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            493..738
                     /locus_tag="IXH04_002570"
     CDS             493..738
                     /locus_tag="IXH04_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1853125.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            835..1179
                     /locus_tag="IXH04_002571"
     CDS             835..1179
                     /locus_tag="IXH04_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853126.1"
                     /translation="MISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFHYSPYRLF
                     QYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHDEVYLAEC
                     LANLEQKGIYLT"
     gene            complement(1240..1803)
                     /locus_tag="IXH04_002572"
     CDS             complement(1240..1803)
                     /locus_tag="IXH04_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853127.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(2371..2571)
                     /locus_tag="IXH04_002573"
     CDS             complement(2371..2571)
                     /locus_tag="IXH04_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ1853128.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT          957 a          553 c          496 g         1120 t
ORIGIN      
        1 gatagataag acacattcag cggaacagtt cctgcttatc agcagaaaac tgttctgctt
       61 tttcattttt cattgatcta ttcttctttt attttcctag catttcattt gtgatgtatg
      121 caataacatg ttatctttta ttatatgaat cgttgaggag tccgttacat gattacgttg
      181 gtgagagttt tgttttgggt gcctgcggtc gcactggtcg caagtattgt ttatcttatg
      241 aattggaaca aagaacggtt ttatttagct attttgactc ttccggctat ctactttatg
      301 tggaaagttt ttaattacaa ctactttgaa cccgatagtg tttttataga agaattatca
      361 ggactggttt tgtctttatt gatcgtcatt ctttatttga tacgtttaaa taaaaaacat
      421 tgagtactat tgattgcatg tgtagctttc tatagtcatt ataactattc tactataatg
      481 aaaggtgatt aaatgagtat cgaagaagct attttactgg atactttacg ttgtcgtctg
      541 aatttcttaa gaactggccg atctttatct caagtagctg tttctagcgc gttaagtatt
      601 cctttattca aatatcgtaa gtacgaagaa ggagaagaaa ttccaagcat cattgaatta
      661 atctatatcg ctgactttta cgatatttcc gttgattacc taattggtcg ctcagaaaca
      721 gcaagcaaac tgaagtgatt ttttccagcc ttggtgctgg ttttttcatt taacgtctaa
      781 ggttaacaat ctatatataa aggagacatt tctatgtgat tatggcatga aaaattgatt
      841 tcaaagcttc ctcgtcaaca gcttttagga caacatcggg aatgctgtgc attaagagga
      901 aatggctggg gaaagaaaca tgctactgtg gattatgttt ttcactattc tccttataga
      961 ttattccaat atcatcagct tgtcatggat gaaatggaaa aacgtggtta cagcccagca
     1021 ccagagtgga aaaatcctat gtatcgtggc aaatcatgcg aagcattttt tgatttacct
     1081 actgtagagc gcaacgtgcc gatttatccg gaacacgatg aagtttatct agcagaatgt
     1141 ttagcaaatt tggagcaaaa aggaatttat cttacctaat ataactagaa aagccttcta
     1201 tctcctctta cttcattaaa aagctttaac catcttagat caagctaaaa tgtcgtaagt
     1261 tttagggtta tcttcatcct cacgtgcaaa actaaaagcg tactctagtt ttggttcatc
     1321 gccaacttcc ctagacataa atgaacatct cctgacgtgg tttaaaagta ccgcatcttc
     1381 gacgtctgta tacgatgcac agcacagatt gcttcttttg atctttcttt tgatttttct
     1441 gttgcttctt ctacaccttc ttcgattact acatcgatgc ttacaattgc ttctgcctaa
     1501 tattcccatg catcgtcgat ttaagcctct gtagtcactg tgatcgtttc cacattttaa
     1561 ctcctctgtc gtggcttcag ttgattttgt gatcagttca gttgctaagc cagagtcaga
     1621 acaacctgcc attgctagcg ttaatattga tagtaaaaat agttttttta tcataaggtt
     1681 ccccccctga aaatagacta aaataataac gctttctttt tttgttttca gacgaaaatt
     1741 aataatttct aaatattctt taaaaaagct tataactatt atgccttttt taatatataa
     1801 cacagttatt tttaaaaata agaatgctaa atcactctac aataacggcg aaagcacaaa
     1861 aaataaaaaa attattacgt tattaaaaaa acagatgaaa gatcaggttt tattagatct
     1921 cattctttca tcacttgaag ggatttaatg aactactttt tcctctttgt cgtttttata
     1981 tattcgtatt ccttcaaaca gtaggatagc ctctttcaat gcttgatatt tgaactgtcc
     2041 gttacttaaa aatttagatg tgccgccctc ttcctagaac atattctttt ttactttttc
     2101 attaattgac agaccaatcg aacgaaactg taaatttcga taggataatg attggtcaaa
     2161 ctcattcatt ttcaagacat ctcgattcat attcaagata ggaatctttt tatcaacatc
     2221 actttattcg tattttcctc taccataaaa gcttcacgct ccacaaaatt gttttataca
     2281 ctactattca gcccattcaa cgaccaatga attttttcgt acaaaaaaac cgtcttttct
     2341 ttcgaaaaga cggttaaaag tcaattttta ttaagcttta acaatgtttg ttgcttgagg
     2401 gccacgttga ccatcttcaa catcgtaaga cactgcttgg ccttcttcta aagttttgaa
     2461 gccgtcacct tggatagctg aaaaatgtgc aaatacatca tttccatctt caccagtgat
     2521 aaagccaaaa cctttgtctg cgttgaacca ttttactgta ccgttattca taaataaatt
     2581 cctcctgata cgtatcaatg tacgtttttt cgttgcaata aatatttgat aggcaaaagg
     2641 agttttatct atgtagatat atcgctcaaa ttacgtttca aaatagatta ctcatttaat
     2701 atatcatgct ttttctaata acacaaggat aatgcaatga aatataaaat tcattgtctt
     2761 ctattgtctt cgaatcgaag aaaaagttga ataaccgcga ttgattcact ttttgttttc
     2821 ttgtttattt gcttttatct gcaacttttg actttaaaat ttcttaacta tgaaaacctt
     2881 tcaattggtg gtatacttat aaatggaatt tcgttatcaa cgcgttcgtt gcctttcagc
     2941 aagtatttaa agaaaggaga atggctatga cattctataa caaagatata caatatcgag
     3001 taaccttgga tacagatttg aatctatttg tcgtttttga caaagaaaat accaatcatg
     3061 tagcgacagg tgttacgatc gaacaagcag tgcaagaatt aaaaaaatct gcataaggag
     3121 tggaaa
//
LOCUS       DACEXQ010000257         3113 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.257, whole genome shotgun sequence.
ACCESSION   DACEXQ010000257 DACEXQ010000000
VERSION     DACEXQ010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.257"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            92..388
                     /gene="yidD"
                     /locus_tag="IXH04_002574"
     CDS             92..388
                     /gene="yidD"
                     /locus_tag="IXH04_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAQ1853129.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            572..1039
                     /locus_tag="IXH04_002575"
     CDS             572..1039
                     /locus_tag="IXH04_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1853130.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            1124..2008
                     /locus_tag="IXH04_002576"
     CDS             1124..2008
                     /locus_tag="IXH04_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1853131.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            2125..2358
                     /locus_tag="IXH04_002577"
     CDS             2125..2358
                     /locus_tag="IXH04_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAQ1853132.1"
                     /translation="MSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            2446..2526
                     /locus_tag="IXH04_002578"
     tRNA            2446..2526
                     /locus_tag="IXH04_002578"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2480..2482,aa:Tyr,seq:gta)
     gene            2533..2604
                     /locus_tag="IXH04_002579"
     tRNA            2533..2604
                     /locus_tag="IXH04_002579"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2565..2567,aa:Gln,seq:ttg)
BASE COUNT         1103 a          459 c          694 g          857 t
ORIGIN      
        1 tgaagcagaa catgacaaaa gaaaacagaa gggaacgttg acgttagtcg cgatcccttt
       61 ttttagtaaa agaaaagaag gaatctaatt gatgaagaag atattgattt atttagttag
      121 aggatatcag cgctttattt cgcctgcatt tccacctagc tgccgttatt acccaacctg
      181 ttccaattat atgataaaag caattcaaac ccatggtgca ctaaaaggga cggtaatggg
      241 gactgcacgg atcttacgct gtcatccttt tgtcaaaggc ggtatcgatt atgtaccatt
      301 gacattccaa ttaacgcgaa ataagaaaga taaaaaagaa ccagattata aaaaggttgt
      361 gaaaaatacg agtagcactg agcgataaag aaacaatttg gaaaatagct tcttatattt
      421 tgcaaaattg tgagttattg ccgaagtgtt ggattttgaa caccgtcaaa aaaaggttgt
      481 gaaaaatacg ggtagcactg agcgataaag aaaaagagga aaattattgt caaagtaaca
      541 actccgcaac tacttatatg ggggacaaaa aatgatcgaa gaactaaaca agaaagattt
      601 accatggtcc ttgttattag atgcagatcc tgataaagaa aaagttcaag tatatgtttc
      661 cagagggagt ggctggatat ggaaagaaaa agaaaaaaca ataggtgtac tgatctatgt
      721 ggcaagggaa aaagaatacg aaatcgtcaa tgtagcagta gccccaacgt atcaaggaaa
      781 ggggattggc ggaaaattac ttgagacagc ttttcagaag ctttcacaac tcgcttccag
      841 tcaaactcag atcatcatac gaaccggcag tactagcagt gcggcgttac atctctatca
      901 aaaaatgggc tatgtggaga ttggtcgagt caaagactat tttatccatc attactctga
      961 gcccattttt gaaaatggag atcaattaag ggatcaagta attttagcaa ttcacttacc
     1021 taaaaatagc tttaactaat aaactttcaa ttcgagaaaa tgctttcaaa tgctaaatag
     1081 atacattaca atagctttag caaacaaaaa ggagaatatt tttatgggaa tattagtttt
     1141 tgattttggt ggatcggctg tcaaatatgg ttgttgggat ggcaaagaga ttaaaggtaa
     1201 gggacaattt gcaacgcctg aatcttggga agaaatgaaa gcgcaattat ttcaagttta
     1261 taaaaaaata ctcgtttctt ttgaagtcga aggaattgga atcagctctc ctggagtagt
     1321 gaataatgag aaacaggtga ttgagggaat cagtgcgatt ccttatattc atggatttaa
     1381 tattttccga gatttggaaa ctctgtttca actcccagta acgattgaaa atgacgccaa
     1441 ctgtgccgga atggctgaat tttatgaagg tgctgcccgt gattatcaag atgttgcttt
     1501 tgttgtggca ggaactggga ttggcggggc gttgttcacg aaaggacaaa tcaataaagg
     1561 ggcacatctt tatggtggag aatttgggtt gatgttctta aatggcgata agacattcag
     1621 taaacttggt acagcggtac agatggcttg gcgttattgt gaacgcaaag gtctcgataa
     1681 aaattcttat accggaaaag atgtctttga attagcagaa actggagatg caatagcaaa
     1741 agaagaagtt gagagttttt atacgtatct tacaaaggga ttattcagta ttcaattttc
     1801 gtttgatcca gaagtgatcg tattaggcgg cggtgtttct gcaaaagaag ggctcattga
     1861 tgagatcaaa agccgaatga agaagctgac agaacaattc gatctgcatg atttcgaacc
     1921 gcagatttta ttatgtgaat acagaaacga tgctaatctt gtaggtgcag cagcaaacta
     1981 tctcgctgta cgtcaagggg agaattaaga gctttagaga aggtgcagct tgcataaaag
     2041 aagtgaatga aatgaatagg agatttttac ctaaggaatg ttttatttgc tgcagtatga
     2101 taaaatatct gagaggtgaa aatgatgtca aaaaaaaata aagatgttga agtgcgtata
     2161 gaagaagtga aaaaagatat caaaggtaag aattatgaag tcactcagtt atttataggg
     2221 aaaaaaatga ttggtgagat cttagcctat gggccaaaag aatatgaaat tttctttggt
     2281 gaagaagatt ttggaaaaga aaaaagtctt gaaaatgcga tagaaacagt gattcgacac
     2341 tggaatctgc acgaataaaa aaatatcaaa aaaactttca ataaagggtt gcaaaaatct
     2401 ggaaaattag gtattataac taagtcaggt tgatacaaac ctttcggagg agtagcgaag
     2461 tggctaaacg cgacggactg taaatccgtt ccttagggtt cagtggttcg aatccactct
     2521 cctccatttc attggggtat agccaagcgg taaggcaagg gactttgact ccctcatgcg
     2581 ttggttcgaa tccagctacc ccagtatacc acaatcgtaa gctctcgtta gttcgagagc
     2641 tctttttttt gctttgctac cttgttcctt gaagattctg ctgatttaag atagagttaa
     2701 gttaactaca agaattggag ggatccacat gaaatggaaa gaaaaagtag acgaagcagc
     2761 tgaaaagtta tatgatttgg tcaaatcaga aaaatacaat attgaagtga atattccgaa
     2821 aaaaggcgga aaagcggtcc aggtaaaatc aaaacgccca acgaatcata caaaaaaatg
     2881 gatggctaaa aaccgctaat aataacaaaa gatccataag tcggcatttt catcgatttt
     2941 agggtctttt gttattttct aggttgaaca tatctaaaaa attgtttctg acaataggag
     3001 taggaagaac gatgtgtgtc taaatcctct gttgatggat agaaaccttt atctttttca
     3061 gttgtgaatc aggtatatac cagttataat agaagaaacg aaaggacggg tgt
//
LOCUS       DACEXQ010000258         3096 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.258, whole genome shotgun sequence.
ACCESSION   DACEXQ010000258 DACEXQ010000000
VERSION     DACEXQ010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.258"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..208
                     /locus_tag="IXH04_002580"
     CDS             <1..208
                     /locus_tag="IXH04_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853133.1"
                     /translation="QTLFNLIKLDAKISSYLFFLIHDEFMDYQKLDQLIERESWEDYY
                     VGLTFSEKLNNYKLIDYKYLSESN"
     gene            825..>3096
                     /gene="esp"
                     /locus_tag="IXH04_002581"
     CDS             825..>3096
                     /gene="esp"
                     /locus_tag="IXH04_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAQ1853134.1"
                     /translation="MISKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLVGVGLVLGTT
                     ELVKAQDEISPSTPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTDNWEIRLQLDERLAKYVENIQVDSKKVAGISYRRTF
                     VRINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKNLDEIFQEIGED
                     NLKSDRLMYRIYLVSHQDNDKIVPGIESTGYFITDQDNFYNKLDVSENNPDQFKHGSV
                     STKYEKPNVQTKDGSGSTGANGAIILDHKLTKEKNFSYSVSATGTPWYANYKIDERLV
                     PYVAGIQMHMVQADRVVYDVSFESGTKVADLAIERREGHENYGVGSITNNDLTKLIDF
                     SNASPRPVVIRYVLQLTEPLDEILAEMKTAAQVEGNEPFGEDFIFDSWLSDTNKKLIQ
                     NTYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAG
                     TGHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSNKLIAKDELKVQIVS
                     PGFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTS
                     NVADSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTIQSGKPTFD
                     KPDVPLANDAFSILDVYNKDFGNASVDANTGIVTFTPAKGVGETEPITGTIPIKIVYQ
                     DGSVGTTDLAVTVSKDI"
BASE COUNT         1121 a          447 c          602 g          926 t
ORIGIN      
        1 gcaaacatta tttaatctga ttaagctaga tgctaaaata tcaagttatt tattcttttt
       61 aatacatgat gaatttatgg attatcaaaa attggatcag ttaattgaaa gagaaagctg
      121 ggaagattat tacgtgggtc taactttttc agaaaagtta aacaactaca agttaataga
      181 ttataagtat ttgagtgaaa gcaattaatt attcatgtag agtactagac actttaaata
      241 tggacaattc aattataaag aaaaagaatc aataatttaa tgaggctgtg acaaatgtca
      301 ttttttgttt cgctaaaaac gagtgaaaaa atgttgataa ctccaatcat tcgtttaaga
      361 ttctaggaat cattcagaaa tgctgtgaaa ccgatacttt ataattataa agaaggtgat
      421 ttcaaattcc gaagaaggaa tttgaagtca cctttttact tatgtcacag tctcattcag
      481 taatgcggga taaggctcct tattccgcta tttgaatact ctgaagttta atctaagaaa
      541 gagtaatatt ttgaaaacat gcttcatcgc atagaaatga acttaatttc agaaagaata
      601 catacatatc tttttatatg ttttcagctg ctgaatggtt aaaaagcaca aatatccgaa
      661 aaaaacatga agaaatgtac tttttttgtt gtaatgtgca tgaaattctt taagtaaatt
      721 agttaacata agtttaataa gattcctatt ctgtgaataa tacgtatcag atttatcttt
      781 tattcttact aggaaaataa ctattaaggg agttgatttg aataatgatt agcaagaata
      841 ataagagagt atttctagaa aaaacaaaga aacgagtatt gaagtacagt atcaaaaaat
      901 taagtgttgg ggttgcatct gttttggttg gtgttggtct tgtcttggga acaactgaac
      961 tagttaaagc acaagatgaa atttctccaa gcaccccact tgaaacagcg ataagctccg
     1021 tacaagtagg tgacaaagta gcttcgggga atacttttca ggagaatcct ggttatacga
     1081 aaaattataa cttttcggat ttacaattta gtcctcaaga actaactgga gacacactaa
     1141 aaggcaatac aattggattt gaagtttacg gaaaacataa tattgctgct tctaccgata
     1201 attgggaaat ccgcctccaa ttggatgagc gattagccaa gtacgttgaa aacattcaag
     1261 tagattctaa aaaggttgcg gggattagtt acagaagaac ttttgtccga atcaatgatt
     1321 cattaggtag acccacgaac atttggaaag ttaattatat tcgtgcaagc gatggattat
     1381 ttgctggggc agaaacaact gatacacaaa ctgctcctaa cggggtaatt acatttgaaa
     1441 agaatctaga tgagattttt caagaaattg gtgaagataa ccttaagagt gatcgtttga
     1501 tgtatcgaat ctatctggta agtcatcaag acaatgataa aattgtacct ggtatagaaa
     1561 gtactggtta ctttataaca gatcaagata atttctataa taagttagac gtatctgaaa
     1621 ataatcctga tcaatttaaa cacggatcgg taagtaccaa atacgagaaa ccaaatgttc
     1681 aaacgaaaga tggatcgggg tctactggtg ctaatggcgc tataatcttg gatcataagt
     1741 taacaaaaga gaaaaatttc tcttactcgg tttctgcaac gggaacgcct tggtatgcta
     1801 actacaaaat tgatgaacgt ttagttccgt atgtagcggg tatacaaatg catatggttc
     1861 aggctgatcg agtagtatat gacgtttcat ttgaatcagg aacaaaagta gctgatttag
     1921 caatcgagcg acgggaaggt catgaaaatt atggtgtagg ttcaataact aataatgatt
     1981 tgactaaact tatcgatttt tctaatgcaa gtccacgtcc agttgttatt cgttatgttc
     2041 ttcaattgac tgaaccatta gatgaaattt tagcagaaat gaaaacagct gcacaagttg
     2101 aaggaaatga accgtttggt gaagacttca tctttgattc ttggttgtcg gatacgaata
     2161 aaaaattaat ccagaacact tatggaacag gttattatta tttacaagat attgatggtg
     2221 atggaaaccc tgacgataaa gaagagagtg gagacacgaa tccatatatc gggaaacctg
     2281 aattagaaga agtatatgat gttgatacaa cagttaaggg gaaagtattc atccacgagt
     2341 tagcgggaac aggtcacaaa gctcaacttg ttgataaaga aggtactgta ttagcagaaa
     2401 aaactattgc tccaaatgaa aaagatggag ctccaatttc ggatactgta gaatttgaat
     2461 ttacgggtgt ggattcaaat aaattaattg cgaaagatga attaaaagtt caaatcgttt
     2521 ctccaggttt tgataaacca gaagaaggtt caaccgttat caaggaatca ccaaaagcgg
     2581 ttgataaaca aaccgtggta gttggattta aaccagatgc taaagaatca attcggaata
     2641 ataaaaactt acctgaagat gcagagtatt catggaaaac agagcctgat acttctaacg
     2701 ttgctgatag tacgaaaggt attgtaactg ttaaaatcgg aaatcgaact tttgacgtag
     2761 atgtagagtt tgctgtaaaa gcttctcaag ctatggaaaa tgatgcaaca tacgtaccta
     2821 taacaacaac cccagaaacg acaattcaaa gtggtaaacc tacatttgat aaaccagatg
     2881 ttcctctagc taacgatgcc ttttcaattt tagatgttta taataaggac ttcggcaatg
     2941 caagtgttga cgcaaatact ggtattgtta cattcactcc agccaaaggg gtaggagaaa
     3001 cggaacctat tactggaaca attcctatca aaattgttta ccaagatggt tctgtaggta
     3061 cgaccgattt ggcagtaact gtaagtaaag atattt
//
LOCUS       DACEXQ010000259         3081 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.259, whole genome shotgun sequence.
ACCESSION   DACEXQ010000259 DACEXQ010000000
VERSION     DACEXQ010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.259"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(202..2811)
                     /gene="clpB"
                     /locus_tag="IXH04_002582"
     CDS             complement(202..2811)
                     /gene="clpB"
                     /locus_tag="IXH04_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAQ1853135.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
BASE COUNT          812 a          658 c          526 g         1085 t
ORIGIN      
        1 gatgagcgtt actttgaaag ttataaatta gaaaaagtga aaaataccta ataaaagagg
       61 ttgtgacaga agcgggcagc ttcaagaaat ttcggcttat ttttcgaaga aactacttct
      121 ggaacactgt ttatttgaaa aaaggaggtc tgacactagt tgtgtcagac cccttttttc
      181 ttttatagtc tcaatggaag attagacgat ttcttctagc tcttctgttt tgaaatgaag
      241 ttgtccatca agtaaagtaa tcgttacttt tgatttaggc ataacatgtc ctgcaacgat
      301 ttctttagct agtggtgttt ctacttcctt agtgataaat cgtttcaatg gtcttgcacc
      361 atatgctggt tcataagcat tttcagcaat ccacgttttg gcttcatcac tgattgtcag
      421 caggatttct tgatgttcca gacgttgtgc caattgcgcc accattttgt caacgattcc
      481 tttcacgttg tctaggctca atggtgtaaa taaaatcgtg tcgtcgattc ggttcaagaa
      541 ttctggtttg aagtttccac gtaatagcgt gttgacttgt tcagccactg cttctggaat
      601 cgttccatca gcagtcacgc cttccagcag taactgtgaa ccgatattgc tggtcatgat
      661 cagaaccgta tttttgaaat ctactactcg cccttttgaa tcagtcaagc gtccgtcatc
      721 taggacttgt aacaagatat tgaagacatc gggatgcgct ttttcgattt catccaacaa
      781 gacgattgta taaggatttc gtctgacagc ttccgttaat tgaccacctt cttcgtaacc
      841 tacatagcct ggaggagctc cgactagacg agacacagca tgtttttcca tatattcact
      901 catatcgatc cgtaccatat gatcttctga gtcaaacaag ttctcagcta acgctttcgc
      961 taattcggtt tttccgacac cagttggtcc taggaaaagg aaagaaccta gaggacgatt
     1021 tgggtcttgc aatccagccc gtgaacgaat taccgcgtca ctgaccgcat caactgcttc
     1081 atcttgacca atcactcgtt tatgaagtgt ttcattcaat ttaattagtt tttctcgttc
     1141 accttctaca agtttcgtta cggggatacc cgttaatctg ccaacgactt gagcaatctc
     1201 attttcggta acggactctt gaaccatctt aatctcgctg tcttttgctt tagcttctaa
     1261 ttccttcaat tctttttcta attgaggtat cgtaccatga cgcaatacag cagcgcgttc
     1321 taaatcatag ttattttctg catcttctaa ttcgtgtttt gctttatcga tttcagcacg
     1381 ttttgctgaa acagaattga cttcttcttt ttctgtttcc cactgcatct tcatcgcatt
     1441 tgcttcttca cgaagttcag ctaattcttc ttgcaaattt ttcaagcgtt ttttacttgc
     1501 atcatctgat tcttttttca gtgcagcttc ttcgatttcc aactgcatca aacgtctagt
     1561 aacttgatct aattccgttg gcatagagtt catttctacc cgaatcgttg cactggcttc
     1621 atcgatcaaa tcgattgctt tgtccggtaa gaaccgatca gtaatataac ggtcagataa
     1681 agtagctgct gctactaatg cgttgtcatg gatgttaaca ccatggtgga tctcaaaacg
     1741 ttcttttaat ccacgcagaa tactgatcgt atcttccact gttggttctt tgactaatac
     1801 cttttgaaag cgacgttcca acgctttgtc tttttccata tattgacggt actcatctaa
     1861 agttgtggca ccgatcaaat gtaattcacc gcgagcaagc attggtttca acagatttcc
     1921 ggcatccata cttccttctg tttttcctgc ccctacgata ttgtggattt catcgatgaa
     1981 caagaggata cgtccatcac tttttttgac ttctttcaag actgctttta gtctttcttc
     2041 aaattcacca cggaattttg ctccagcgat caatgcaccc atatctaatg aaaaaatcgt
     2101 tttatctttt aaattttcag gcacatcttt gcgtacgata cgttgagcaa gcccttcaac
     2161 gattgccgtt ttccctacac cgggttctcc tatcagaaca ggattatttt ttgtttttcg
     2221 tgaaagaata cggatgacat cacggatttc ttcatcacga ccgatgattg gatccatttt
     2281 tccgcttttg acttgctgta ctaaatcaac gccgtatttt tctaatgctt tgtattgttc
     2341 ttcttggttt tgtgaggtca ctctttctcc tcctctcatc tcttcgatgt ttttacgcaa
     2401 ttctttttca gataatccgt ttttattcaa ataaacagtt aacggataat tttttaattt
     2461 catcaacgct aaaataacga cttccgttga aagaaactcg tcgccgaagc tttcacgtat
     2521 ttggtctgct tcattcaata atcgaaagaa attctggctg aggttttgtc catattggac
     2581 gtttccacca gaaacaactg gatactcatc cagtagacga tcaatttcat gttcaaaaga
     2641 ttcgacatcc aatccggcat ccgtatagaa atttcgacca aaatgattag gttgtaaaaa
     2701 gattttccat acatgggcaa tgtcgatatc ttgatgtttt cgagtgaccg caatctgttg
     2761 agcttcagca atcgcttctt gaagcgtcgt agtcattttc tcaatattca tacaggtata
     2821 ccctcctaat taaaggtaag atcacttatt tctttgatct ttacaaaaac tattatatcg
     2881 ctttggtcaa ttttggtcaa aatatacaca tggttttttg actttttttc tcgacttata
     2941 taaatcttaa aaaaacaaaa ttgattgtat gtatataaaa aaagagccaa acaaacgtaa
     3001 aattatctta tccataacgt ttttctttga tttgataaaa agggtagatc ttataaaaga
     3061 cctacccttg attttcttta t
//
LOCUS       DACEXQ010000260         3073 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.260, whole genome shotgun sequence.
ACCESSION   DACEXQ010000260 DACEXQ010000000
VERSION     DACEXQ010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.260"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..1002)
                     /locus_tag="IXH04_002583"
     CDS             complement(<1..1002)
                     /locus_tag="IXH04_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ1853136.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRIGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LIASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWITFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFTTIPKKNSKSFRE
                     AVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRL
                     "
     gene            1676..2488
                     /locus_tag="IXH04_002584"
     CDS             1676..2488
                     /locus_tag="IXH04_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853137.1"
                     /translation="MILLAVSMSLLIYTHILSTFMSVIVIGFLFIGSLFSKRKERKEM
                     SNLISNMIKAVGWTILLTAYVWYPMLQQVLHQKINRPFRTKLQERALNISDSLIGAVN
                     NDLVTFTMGTIGVVSFILPLFFKKKFANKEKIINFCAVTTWFLSTNLFPWEFLQKTPI
                     QIIQFPWRILGFQVLFGSLILIIVFLKWKTSNKKSMCSLVGIVLLIFTVTVATEANYS
                     QKIQSYKGRLIMTKKDVTLYTTSRTGGLYDYAPLDALKYKDHLKKHEVKVYD"
BASE COUNT          977 a          466 c          519 g         1111 t
ORIGIN      
        1 tagtcgattg tgggaatttc cttgtacggt atattgaaag gcgtcttcga atccatcatc
       61 caatgatgcg caagcttttg aatattttgg ttgatcgata taatcatgaa tcaatcgatt
      121 tttagcctca cgagctaagt taatatctgt gaacttaaaa attcctttaa cagcttctct
      181 gaaagatttt gaattttttt taggaatggt ggtaaagata tttcttagga agtgaacttg
      241 gcatctttgc caacttgcgt tggtgaagga ttttctaatg gcagagacta atcctttgtg
      301 cgcatcagaa ataacgagtt ctgtaccttg taaaccgcgt tcttttaggt attcaaaaaa
      361 tgttatccag gtctcttcgc tttcgccact ttgaatcatg aagccgataa tttcacggtc
      421 gccatctttg gttattccaa tcgctatatg acagcttttt gagagtactc gattttcttc
      481 tcgtactttt atatagagta catcggtcat taagtaagga taattttttt ctgataataa
      541 acgattctgc cactcgttaa ccattggttc tagctgttct gttaagctag aaacgaagga
      601 cttagagacg gatttaccac aaagttcttc cacaattttt gatactttac gagttgaaac
      661 gcctgataca tacatttcca acattgaagc aatgagggct ttttcgtttc gttgataacg
      721 ttcaaacact gtgggtgaaa aatggccatc acgtgttctg ggtactttta attctagcgt
      781 gcctatacgt gtcgtaaagc tgcgctcata atagccattt cgttgacttt gtcggttttc
      841 tgttcgttca tattcttttg cttgaatata ttctgttcgt tgattttcca ttagttgatt
      901 aaataccgtt gttaaaatat ttttagaaac gtcatccttt acagaatatt caataatgct
      961 ttgaatctct tcgcttttca gtgtaaaatg tacttgggtc atgtaaaagt cctcctgggt
     1021 atgtttttgt cgttaaaaac attgtaccgt aaaaggactg ttacatggcc tttttacttt
     1081 tacacaatta taaggacttt atcgaaaaaa agcagttatt tgttaaaatc attgatatta
     1141 ttaataataa gtattttttg aaaggggaaa tataaaaggt ttttgaaaat aaataaaaaa
     1201 tagtattact tgttctattg ctagttattg ctagtcttta ttcttttttg ttttttaggg
     1261 atgtacaaaa gatctcaacc acaggagaca ttgcttttca cttttctaga ataaaatgct
     1321 tgtcctcagt attttctggt ccaataaatt ttaccacatt taatagctat ggcggaggcg
     1381 tgaatttttt tatccatatc taacatcttt ccagccgtaa tattttactg gacaacaaac
     1441 aatttgattc taagttatat acttactata cgttgggata ctgaatatct gtacaattct
     1501 agtgagctat caatatggac aaaagaatag atgcagcctt cttattcagc tgtttgtata
     1561 gcttttatgg attgatatat attagcgtac tgcaatttca gaatcaattg cgttgacgat
     1621 tgtaccagtt gtcttgtatt acacatatac tcttctttat gaaagaaaaa actgcgtgat
     1681 tcttttagct gtcagtatga gtttgctcat ctatacccat attctctcca cctttatgag
     1741 tgtgattgtt attggcttct tgtttatcgg ctctcttttc tctaagcgga aagagagaaa
     1801 agaaatgtcg aatctgatat caaatatgat aaaagctgtt ggttggacca ttcttttaac
     1861 agcttacgtt tggtatccga tgttacaaca agtcctacat caaaaaatta atcgtccttt
     1921 tcgcacaaaa cttcaagaaa gggcattaaa catttctgat agtttaatcg gtgcagtaaa
     1981 taatgattta gttacattta caatgggaac aattggtgtg gtttcattta ttcttccact
     2041 tttctttaaa aaaaaatttg ccaataaaga aaagattatt aatttttgtg cagtgaccac
     2101 ttggttctta tcaacgaatc tttttccatg ggaattttta caaaaaacac ctattcaaat
     2161 cattcagttt ccttggagaa tcttgggttt tcaagtgctt tttggttcat tgatcttaat
     2221 tatagtgttt ttaaagtgga aaacaagcaa caaaaaaagt atgtgtagcc ttgtaggaat
     2281 agtccttctt atttttacag tgactgtagc aacagaagcg aattattcgc agaaaattca
     2341 atcgtataaa ggtcgtttaa ttatgaccaa aaaagatgtt acactttata caacctcgag
     2401 aactggaggt ctttatgact atgcaccatt agatgcattg aaatataaag atcatttaaa
     2461 gaaacatgaa gtaaaagtct atgattgatg ggagaatgca tctcataaag cacataattc
     2521 taaaataatc tatacagtag gagatgcgaa tgagcaaaat atcacgttac ctgtatttaa
     2581 ttactgggaa cagttatgaa agtaaatggg aaaaagagca ctatagaaaa tgacaatggt
     2641 ttagtgtcta ttaaaggtac gggaaaaaga actatagttg aggtttcatc tacgtatcca
     2701 actacgatga ttctatcttt attaattagt tcaataactt ttatattctt atttacacgt
     2761 tatttaataa gatgctgcgg ttaaatgaaa atagattaga tagatattat aaatatgttg
     2821 attctatttt catagcaatg gagaaaatac attagcaata tataaaagaa ggtatcaaga
     2881 gtcacggtga atttattccg aaaagtgaaa agaacaaata taaaatttaa taaaaatata
     2941 gaactaaata ggaaaacgta gacgtaattt tctacgtttt ccttttcata tatattaaat
     3001 attacggaaa tttatataaa ctaatttagt gttttgtttg taattcctaa ttttgctcca
     3061 cttttagata att
//
LOCUS       DACEXQ010000261         3027 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.261, whole genome shotgun sequence.
ACCESSION   DACEXQ010000261 DACEXQ010000000
VERSION     DACEXQ010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.261"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(1056..2396)
                     /gene="glnA"
                     /locus_tag="IXH04_002585"
     CDS             complement(1056..2396)
                     /gene="glnA"
                     /locus_tag="IXH04_002585"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAQ1853138.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(2424..2816)
                     /locus_tag="IXH04_002586"
     CDS             complement(2424..2816)
                     /locus_tag="IXH04_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1853139.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT          929 a          640 c          481 g          977 t
ORIGIN      
        1 atacaagctg actaagggaa agtcaagtac tcaaatgaat actatccgct tacaattttt
       61 tctttttcga tatcctttga gaaattattt cttaagtcct ttatgagtgc aaaaaaaatt
      121 taacccacat aataagcttg atataaacat agctaacgtt aaacctaagc aaatcagaaa
      181 atcgttactg aaaaatttta agtgaaataa tagataaaaa aataaaacaa acattatgat
      241 agataatatc ccacctaaca aagaactact ttttcctact tgcagcaaca gtttatatag
      301 cttatcgtag aaacaatctt tttctttcac ccttatttac ctcctttacg taaaaaacgt
      361 agtccacagt tctcggattt ttggcatatc ttttctatta aataatatca aaaacaaaac
      421 aattccacta attttttcaa agatgacctt tttttcaaca aaagtgcctt gtagggattc
      481 attgaaggta acgaatcaca taagtaaaaa ataaggagat gcgacaaaag tcgtgcctcc
      541 ttatttctaa ataaacggtg ttctcctgcc aactcctcgg tcacaagtca caatatccat
      601 caaacatgag aagctgtttt tgaaattatt tcttgtgatt cggagttaaa tggtaggctc
      661 acaacctctg gataaacggt attcaaaagc cagtttctcg gtattaagcc aaatttatgc
      721 aaaaatgtag aaagccattt tcacaaaatt tttcttaata ctcgaaacta aacgtctttt
      781 tcaaaacctc ttggtaaacg gtgttcaaaa gcttgcatct gcggtaataa gttcagccaa
      841 ttcaaaaaca agaaaagctg tttttgcttg gctattctta ttacttgttg cagggcagct
      901 ttttcacaac ctctccacta aacggtgttc aatcagctga ccctcggtat tagctcaaaa
      961 aacggcaaaa tatgaggaac tatttttccg ttagtttctc taatactcag gtcaaaacgc
     1021 tgatttcaca gcctctaatt cacggtattc ctgttttaat aaagttctag atattgttct
     1081 ctttcccact cggaaacagt ttgtctgaat gccgcccatt cgagacgttt tgcttcaaca
     1141 aaatttgaga agatatgttg tcctaatgcg tcaatcatta cattatcctt acgcaattct
     1201 ttgatagcat tatggatagt tgaaggtaag tcctggatat gtgcagcttc tctttcttcc
     1261 tcatccatta cataaatgtt acggtcaaca gctgctggcg gtgtgatctg attacggata
     1321 ccgtctagtc ctgcttgcaa caatactgcc attgccaagt atgggtttgc agatggatca
     1381 actgaccgca attccaaacg tgtggataaa ccgcgagatt ctggtacacg tactaatggc
     1441 gaacgattac ggccactcca tgcgacataa acaggcgctt cataacctgg aacgagacgt
     1501 ttataagaat tgactgttgg attgcaaacg gctgtgtagg cacgagcatg tttcagcaaa
     1561 ccacctaaga aatgataagc tgtttcactt agttccaatt caccgtcttt atcatagaaa
     1621 acatttcctt catcattgaa caaagacata ttgcaatgca taccagaacc attgatccca
     1681 aataacggtt tcggcatgaa agtcgcgtga agtccatgct tgcgtgcaat cgttttgaca
     1741 acgagcttga aggtttggat attatcacaa gcatctacca catctgcata tttaaagtca
     1801 atttcatgct gtcctggcgc tacttcatga tgtgaagctt ccacttcaaa gcctagactt
     1861 tctaattcca acacgatgtc acggcggcaa ttttcaccta aatctgttgg tgcaaaatca
     1921 aaataaccac ctttatcatt caaggtcgtt gtgatcccac cgtcttcgtc taatttaaat
     1981 aagaagaatt ctggttcagg tcctaaattg aaagaggtaa aaccaaggtc gcgcatatct
     2041 ttcaatgctc gtttcaaatt cccgcgagga tcgccggcaa atggtgtccc atccggatta
     2101 tagatatcac aaattaaacg agcaactttt ccatgagcac tttcccacgg gaagatcatc
     2161 caagtggaga gatctggata caaatacata tcgctttctt caattcgaac aaatccttca
     2221 atcgaagaac cgtcaaacat cattttattt tctaatactt tgtcgatctg gctcactgga
     2281 acttctacat ttttgatcgt acctaagatg tctgtgaaca tcaatcgtaa aaatcgtacg
     2341 ttttcttcct caatgattcg tttgatttct tcacctgtta tctgtttctt taccatggta
     2401 tccctcttcc ttttttaatt tattcagtca aagaattgtc tttgcaatcc ttgcgactga
     2461 taatgacctg ttttgcttaa cccaccttgc gccaaaatct catcacgaaa aatttgtcga
     2521 acgtcatgat ctgtcaacgg cttcttgttt tttgcttctt gttttttcgc ctgtgccatt
     2581 tcgtatacac gtttgatccc agccatattc aatccctctg aaagatagtc tttaatctca
     2641 agcaacatat caatatcatt cagcgaatac atacgtcggt ttccttcgct tcgttccgga
     2701 tgaactaaat cttgttcttc ataatagcga atttgccgag cagacaaatc agtcagcttc
     2761 ataactgtac caattggaaa tacggacata gatcggcgca attccttttc tcccatcaga
     2821 cgtccccttt cgttttataa gttggctaaa gtgtaccaat taaaaaaaat attgtcaact
     2881 gcatatgtta ggttttctaa catgagatct ctatttttta taaaataaca aaaaaataag
     2941 aacacaaaaa aggtatctag ttgattttcg taccagatac cttttatttt ttattatact
     3001 gtactatcct ttattagtgt ttttatt
//
LOCUS       DACEXQ010000262         2974 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.262, whole genome shotgun sequence.
ACCESSION   DACEXQ010000262 DACEXQ010000000
VERSION     DACEXQ010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.262"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(14..706)
                     /locus_tag="IXH04_002587"
     CDS             complement(14..706)
                     /locus_tag="IXH04_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1853140.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEIGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            791..967
                     /locus_tag="IXH04_002588"
                     /pseudo
     CDS             791..967
                     /locus_tag="IXH04_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1143..1712)
                     /locus_tag="IXH04_002589"
     CDS             complement(1143..1712)
                     /locus_tag="IXH04_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ1853141.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1786..2109)
                     /locus_tag="IXH04_002590"
     CDS             complement(1786..2109)
                     /locus_tag="IXH04_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853142.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2263..>2974)
                     /locus_tag="IXH04_002591"
     CDS             complement(2263..>2974)
                     /locus_tag="IXH04_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853143.1"
                     /translation="LKIDVKTVDRHLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYL
                     LFVFLIQSPPFCLLKNLLLNEPYEQKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSER
                     KVRYLLFCFLKDFPHFFADDGEQSFSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPE
                     LLFREEQILPIKEKNYLAILRLSCNQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLN
                     SEFESESKSKLTKVLYQE"
BASE COUNT          817 a          602 c          531 g         1024 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acctatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aacgtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtgtgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1021 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1081 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1141 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1201 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1261 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1321 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1381 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1441 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1501 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1561 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1621 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     1681 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     1741 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     1801 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     1861 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     1921 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     1981 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     2041 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     2101 taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa
     2161 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     2221 ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac
     2281 ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     2341 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     2401 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     2461 tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt
     2521 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     2581 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     2641 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     2701 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc
     2761 gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga
     2821 ttggatcaga aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg
     2881 aacggttttc ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag
     2941 atgccgatcc actgttttaa catcgatttt cagt
//
LOCUS       DACEXQ010000263         2918 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.263, whole genome shotgun sequence.
ACCESSION   DACEXQ010000263 DACEXQ010000000
VERSION     DACEXQ010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.263"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            205..1173
                     /locus_tag="IXH04_002592"
     CDS             205..1173
                     /locus_tag="IXH04_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAQ1853144.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1166..2197
                     /gene="vanA"
                     /locus_tag="IXH04_002593"
     CDS             1166..2197
                     /gene="vanA"
                     /locus_tag="IXH04_002593"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAQ1853145.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2203..2811
                     /locus_tag="IXH04_002594"
     CDS             2203..2811
                     /locus_tag="IXH04_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAQ1853146.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          863 a          559 c          708 g          788 t
ORIGIN      
        1 taatttttta ggaaaatctc aaggttatct ttactttttc ttaggaaatt aacaatttaa
       61 tattaagaaa cggctcgttc ttacacggta gacttaatac cgtaagaacg agccgttttc
      121 gttcttcaga gaaagatttg acaagattac cattggcatc cccgttttat ttggtgcctt
      181 tcacagaaag ggttggtctt aattatgaat aacatcggca ttactgttta tggatgtgag
      241 caggatgagg cagatgcatt ccatgctctt tcgcctcgct ttggcgttat ggcaacgata
      301 attaacgcca acgtgtcgga atccaacgcc aaatccgcgc ctttcaatca atgtatcagt
      361 gtgggacata aatcagagat ttccgcctct attcttcttg cgctgaagag agccggtgtg
      421 aaatatattt ctacccgaag catcggctgc aatcatatag atacaactgc tgctaagaga
      481 atgggcatca ctgtcgacaa tgtggcgtac tcgccggata gcgttgccga ttatactatg
      541 atgctaattc ttatggcagt acgcaacgta aaatcgattg tgcgctctgt ggaaaaacat
      601 gatttcaggt tggacagcga ccgtggcaag gtactcagcg acatgacagt tggtgtggtg
      661 ggaacgggcc agataggcaa agcggttatt gagcggctgc gaggatttgg atgtaaagtg
      721 ttggcttata gtcgcagccg aagtatagag gtaaactatg taccgtttga tgagttgctg
      781 caaaatagcg atatcgttac gcttcatgtg ccgctcaata cggatacgca ctatattatc
      841 agccacgaac aaatacagag aatgaagcaa ggagcatttc ttatcaatac tgggcgcggt
      901 ccacttgtag atacctatga gttggttaaa gcattagaaa acgggaaact gggcggtgcc
      961 gcattggatg tattggaagg agaggaagag tttttctact ctgattgcac ccaaaaacca
     1021 attgataatc aatttttact taaacttcaa agaatgccta acgtgataat cacaccgcat
     1081 acggcctatt ataccgagca agcgttgcgt gataccgttg aaaaaaccat taaaaactgt
     1141 ttggattttg aaaggagaca ggagcatgaa tagaataaaa gttgcaatac tgtttggggg
     1201 ttgctcagag gagcatgacg tatcggtaaa atctgcaata gagatagccg ctaacattaa
     1261 taaagaaaaa tacgagccgt tatacattgg aattacgaaa tctggtgtat ggaaaatgtg
     1321 cgaaaaacct tgcgcggaat gggaaaacga caattgctat tcagctgtac tctcgccgga
     1381 taaaaaaatg cacggattac ttgttaaaaa gaaccatgaa tatgaaatca accatgttga
     1441 tgtagcattt tcagctttgc atggcaagtc aggtgaagat ggatccatac aaggtctgtt
     1501 tgaattgtcc ggtatccctt ttgtaggctg cgatattcaa agctcagcaa tttgtatgga
     1561 caaatcgttg acatacatcg ttgcgaaaaa tgctgggata gctactcccg ccttttgggt
     1621 tattaataaa gatgataggc cggtggcagc tacgtttacc tatcctgttt ttgttaagcc
     1681 ggcgcgttca ggctcatcct tcggtgtgaa aaaagtcaat agcgcggacg aattggacta
     1741 cgcaattgaa tcggcaagac aatatgacag caaaatctta attgagcagg ctgtttcggg
     1801 ctgtgaggtc ggttgtgcgg tattgggaaa cagtgccgcg ttagctgttg gcgaggtgga
     1861 ccaaatcagg ctgcagtacg gaatctttcg tattcatcag gaagtcgagc cggaaaaagg
     1921 ctctgaaaac gcagttataa ccgttcccgc agacctttca gcagaggagc gaggacggat
     1981 acaggaaacg gcaaaaaaaa tatataaagc gctcggctgt agaggtctag cccgtgtgga
     2041 tatgttttta caagataacg gccgcattgt actgaacgaa gtcaatactc tgcccggttt
     2101 cacgtcatac agtcgttatc cccgtatgat ggccgctgca ggtattgcac ttcccgaact
     2161 gattgaccgc ttgatcgtat tagcgttaaa ggggtgataa gcatggaaat aggatttact
     2221 tttttagatg aaatagtaca cggtgttcgt tgggacgcta aatatgccac ttgggataat
     2281 ttcaccggaa aaccggttga cggttatgaa gtaaatcgca ttgtagggac atacgagttg
     2341 gctgaatcgc ttttgaaggc aaaagaactg gctgctaccc aagggtacgg attgcttcta
     2401 tgggacggtt accgtcctaa tcgtgctgta aactgtttta tgcaatgggc tgcacagccg
     2461 gaaaataacc tgacaaagga aagttattat cccaatattg accgaactga gatgatttca
     2521 aaaggatacg tggcttcaaa atcaagccat agccgcggca gtgccattga tcttacgctt
     2581 tatcgattag acacgggtga gcttgtacca atggggagcc gatttgattt tatggatgaa
     2641 cgctctcatc atgcggcaaa tggaatatca tgcaatgaag cgcaaaatcg cagacgtttg
     2701 cgctccatca tggaaaacag tgggtttgaa gcatatagcc tcgaatggtg gcactatgta
     2761 ttaagagacg aaccataccc caatagctat tttgatttcc ccgttaaata aacttttaac
     2821 cgttgcacgg acaaactata taagctaact ctttcggcag gaaacccgac gtatgtaact
     2881 ggttcttagg gaatttatat atagtagata gtattgaa
//
LOCUS       DACEXQ010000264         2853 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.264, whole genome shotgun sequence.
ACCESSION   DACEXQ010000264 DACEXQ010000000
VERSION     DACEXQ010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.264"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..1024
                     /locus_tag="IXH04_002595"
     CDS             <1..1024
                     /locus_tag="IXH04_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1853147.1"
                     /translation="FQWLFFGIVCILIVPITFFITSLTTPKILAVDLLMLFFLSGIIF
                     VSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNCDIAFENVSFSYK
                     EECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQIKIGGKPLNHYSK
                     DTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGCESILEKFPEREH
                     TLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEYELQKAFQYLMKE
                     KTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHRVYKQLVTNYENANDWRL
                     DNEKFL"
     gene            978..2750
                     /locus_tag="IXH04_002596"
     CDS             978..2750
                     /locus_tag="IXH04_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1853148.1"
                     /translation="MRMQMIGGLTMKNFYKKKFALTDQGAEALTKASISSFFVYCINM
                     VPAFIIMMLIDELVLENAKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     VQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVTMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
BASE COUNT          987 a          404 c          532 g          930 t
ORIGIN      
        1 ttttcaatgg ctattttttg gcatagtttg cattttgata gttcctataa ccttttttat
       61 aacttctctt accactccca agatattagc tgttgacttg ttgatgttgt tttttttaag
      121 tggaattatt tttgtttctt tcatgagaat aatgtatgta agtatgcact tgtttgaagc
      181 aagatatgca gtcgaaacac tagactccct ttatttagaa atgaagcaag accaactaaa
      241 atatgggaac gagacacaga ttaaaaattg tgacattgca tttgaaaatg tcagcttttc
      301 atacaaagaa gagtgtgttt tagaaaattt caacttagtt ttagaacaaa aaagaattta
      361 tgcattagta ggaaaatcag gaagcggaaa gagtacgata gctaaactaa tttcaggttt
      421 ctacaatgtg gataagggac agattaaaat tggtggtaag cctttaaatc actattcaaa
      481 agacacaatt attaacacaa ttgcttttgt ctttcaagat acgaaactat tcaatcagag
      541 tatttacgat aacgtttcta tagcaaatcc tcaagcgcca gcaagtaaag tatttgacgc
      601 gctaagatta gccggttgcg aatcgatttt agaaaaattt cccgaaagag agcatacctt
      661 aattggtagt aaaggagttt atctgtcagg aggagaaaaa caacgtatag ctattgccag
      721 agcaatttta aaagatgcaa agattgttat tatggatgaa gccagtgcat ctattgatcc
      781 agataatgag tatgaactac aaaaggcttt tcaatatttg atgaaagaaa aaacagttat
      841 tatgatagct catcgattat ccagcattaa atctgtagat gaaattcttg tcttgtcaga
      901 aggaaaaata gtagaacgtg gaaatcataa tgaattaatg gctaaacatc gagtgtataa
      961 acaattggtc actaattatg agaatgcaaa tgattggagg cttgacaatg aaaaattttt
     1021 ataaaaaaaa atttgcttta actgatcagg gcgcagaagc tttaactaaa gcttctattt
     1081 ctagtttttt tgtatactgc attaatatgg taccagcatt cattataatg atgctgatag
     1141 acgaattagt tttagaaaat gcaaagcccc gttggcttta ttttgcagta tcgtttgtta
     1201 ctttgctttt catgtattgg ttgttagata gggagtatga aaacttatat aatagtactt
     1261 ataaagaaag tgcgcattta agagtgcaaa ttgcagacga tttgtcaaat ttaccattat
     1321 cctatttttc aaaacataat ttatcagatt tatctcaaac tatcatgtct gacgttgaag
     1381 gtattgagca tgcgatgagt catgcaatac ctaaagccgg cggtatggct ctgtttttcc
     1441 cttttatttc agtgatgctt ttggttggta atgtcacaat gggattagct gttattttgc
     1501 caacgttatt tagttttgtc ttaatcttgt tatcaaagaa atcccaaacg aaagccaata
     1561 ctaaatatta cgatactttg agagaaaact cggaagaatt tcaagaaact attgaattgc
     1621 agcaagagat taatagcttt aatctatcta aaaaagttca agacagactt ttcaaaaaaa
     1681 tggaagagag tgaaaggatt catttaaagg tagaattaag tactttttca gtcatggcct
     1741 tatcctctat tttctcatat gttagtttag cagtagtcat tctagtaggt gttcacttac
     1801 tgttaacggg tgaagtgact atactctacg tcgttggtta cttactagcc gcaataaaaa
     1861 taaaggattc cttcgattct atgaaagaag cagtgcttga aatattttat ttagctccta
     1921 aaatacagcg aattagagct atgaaggaaa ccagtatcca agagggatca gatagtcctc
     1981 tgaaatcttt tgacattgag ttgcgagatg tctctttctc atatgataac aataccccaa
     2041 ttttagatca tatctctttt acggctaaac aaggagaagt gactgcgtta gtaggtgcta
     2101 gtggttccgg gaaaactagt atcttgaaat tagtgtctag actgtatgat tatgatgaag
     2161 gctgtatttt aattgatggg tatgatatta aacgtgtatc tccagcttct ttgtttagca
     2221 aaatcgctat tgtttttcaa gaggtaactt tgtttaatac aagtattttg gaaaatatac
     2281 gtattggaaa tagtcaagcg tcagatgagg aagtgaaaaa agcagctcgt ttagctaact
     2341 gcgaagactt tattgaaaaa ctacctgacg gttatcatac attggtcgga gaaaacggaa
     2401 gttcactttc tggaggagag cgacaacgcc tatccatagc acgggctttt ttgaaaaatg
     2461 cgccaatttt aattttagat gaaattactg ccagtttaga tgcggaaaat gaaaaacgta
     2521 ttcaggaaag tttaaatcgt ttaatacaag ataagacagt actaattata tcgcatcgct
     2581 tgaagtctat tgaaaaggta aataaaattg tagtgatgga tcaaggaaaa ctagttgatc
     2641 aaggtactca taatgagttg tatcgaaggt cagaaatata caaaaatctc ataaagaaaa
     2701 ctaaactatc tgagaaattc gtctatgaaa aagaagctca atcacgatga tgaaataggc
     2761 tttaaattaa cctacaattt ttgggatata gttataattc tttttcaaaa agaataattt
     2821 tattaaacag catctaattt tgaatacacg aaa
//
LOCUS       DACEXQ010000265         2763 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.265, whole genome shotgun sequence.
ACCESSION   DACEXQ010000265 DACEXQ010000000
VERSION     DACEXQ010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.265"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..614
                     /locus_tag="IXH04_002597"
     CDS             <1..614
                     /locus_tag="IXH04_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1853149.1"
                     /translation="GGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFI
                     KQSLEEFIQSNETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPI
                     EKQLGMIEEDPNTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYA
                     KDPVVTRYIRKLFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(636..1790)
                     /locus_tag="IXH04_002598"
     CDS             complement(636..1790)
                     /locus_tag="IXH04_002598"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAQ1853150.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            1975..>2763
                     /locus_tag="IXH04_002599"
     CDS             1975..>2763
                     /locus_tag="IXH04_002599"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HAQ1853151.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNN"
BASE COUNT          889 a          510 c          557 g          807 t
ORIGIN      
        1 ctggaggatc tggcagattt ctttccttag caaaagaaat taatcgctat gatttgatcg
       61 atttttcttc aggaatgcta gaaaatgcaa aaaaagaagc agaaaaaaat gagttgccaa
      121 atgtttcctt tatcaaacag tcgttagaag aatttatcca atcaaatgag acatatgaaa
      181 tggtttttac agcagcaaat ccagcattgg tacatcttat tcaattagaa aaattgatga
      241 aaaagtcaag caaatggtgc gtgataatca gagtagtgga atcaatcgat gatgtttttt
      301 atcctatcga aaagcaacta ggaatgatag aagaagatcc taatacatcg ccagttgtaa
      361 tggatcaatt tgaaacatac ttaattgatg aaggattcaa agtgaagaaa aaagatttta
      421 cttattttac acaggaatca attactgaag aattgatgaa gctatactat gacgcgtacg
      481 caaaagatcc tgttgtgacc aggtacattc gcaaactatt cagtgaaaaa cgagaaatcc
      541 ttagtacgac taagctgacc tatagactgc tcctttggtc cgcagataaa aaaagaacct
      601 ttcacgactc gtgaagggtt ctttttatga ttttattata ttttgtagta acgaatatca
      661 ttttcgatct ttgtaatcga aaaaggtaca tctgaagtat attctgcaca ttctccgtgt
      721 tcaggaagcg tctcactgaa aatcgtttca tattcgtcaa cagtgatccg tgttcgagaa
      781 tctagcatct cttgatgaga ttgtgcgaag agatattctt gatagttttc ttccagataa
      841 cctgtaaaaa attcacttac tgcaccagaa ccgtagctga atagaccgat gcgatctcct
      901 ggctgtagtg atttcgaatt ttccagtaga gaagttagcc ccaggtataa tgaaccagtg
      961 taaagattac cgattcgtcg gctgtaacgg atgctttctt catagcgagc cataagacgt
     1021 tcctgatttt cttcgtctgt ttggtctaat acgctttgca atgccttttt tcccatctta
     1081 gtatacggaa tatggaaagc aatcgcctga taatcttcga gtcctcgacc cgacaattct
     1141 ttatgtcgat tccaaacttt ttggaatgat tcgatatacg tagaattaga taaaggacca
     1201 tcaacaacag gaaattcgct ataatctgga cgccagaaat catagatatc ttctgtcaga
     1261 aatacgctgt cgtcttcaat cgataaaata cgcgggtttt gagtgatcat catcgcaaca
     1321 gcaccgacac cttgcgtcac ttcaccaccg cttgccaagc cgtaacgagc aatatcactt
     1381 gctatgacta gtacttttcg ttctggatga tttttgacat attctttcgc catgtgcaaa
     1441 gctgcggtgc caccgtagca agcctctttt acttcaaaag aacgagcaaa agattggatt
     1501 ttcagtaaat ggtggataat taccgcactc gcttttgaat gatcaatccc ggattctgtt
     1561 ccgacgatga ccatgtcgat tagctcacgg tctttttctg ttacgatttt gctggcagca
     1621 tttgctccta gtgtaatgat gtcttcattt gcacgattga ctgccatttg gtcttgacca
     1681 atccctatat ggtattttgc aggatcatcc ccacggcttt ctgccaactc tgtcatatct
     1741 aaatataaat taggaataaa aaaggaaaga cgatcaatcc ctattttcat ttcaacacct
     1801 ctttacttat gataaaaaac ttactttact aaggtatcat aaaaagatta aaagaaatat
     1861 aaaaaagaga atatttaagg attgttaaaa tttaatctta gaaaaaaata actggaacaa
     1921 gagctatttg tttttacagc aaagaatggt atatttttgt aggaggtggc ttacttgaaa
     1981 gaagtcgtta tgatagatgc tgcaagaacg ccgataggaa agtatcgcgg aagtttaagt
     2041 ccatttactg cagttgaatt aggcacactt gtgactaaag gattattaga taaaacaaaa
     2101 ctgaaaaaag ataaaatcga tcaagtaata tttggaaatg tcctgcaagc tggtaatggt
     2161 caaaatgttg ccagacagat tgctttgaac agcggtctcc ctgtagatgt cccagcaatg
     2221 acgatcaatg aagtttgcgg atctggtatg aaagcagtga tcttagcacg ccagctgatc
     2281 caattaggtg aagcagaact tgtgatagcc ggcggaacag agagtatgtc tcaagcacct
     2341 atgctgaaac agtatcagtc agaaacaaat gaatatggtg aaccaatttc cagtatggtc
     2401 aacgacggat tgactgacgc attttcaaat gcacatatgg gattaaccgc agagaatgtt
     2461 gcaacacaat tttctgtgag cagagaagag caggatcgct atgccttgtc gtcccagttg
     2521 aaagcagcac atgctgtcga agccggtgta ttttctgagg agatcatccc agtcaagatt
     2581 tctgatgaag acgtgttatc tgaggatgaa gcagttcgtg gaaatagtac attggaaaaa
     2641 ctgggcacgt tacgtacagt attctcagaa gaaggaactg taacagcagg aaatgcttcc
     2701 ccgttgaatg acggtgcctc tgtggtgatc cttgcatcca aagaatacgc agaaaataat
     2761 aat
//
LOCUS       DACEXQ010000266         2736 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.266, whole genome shotgun sequence.
ACCESSION   DACEXQ010000266 DACEXQ010000000
VERSION     DACEXQ010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.266"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(137..1528)
                     /locus_tag="IXH04_002600"
     CDS             complement(137..1528)
                     /locus_tag="IXH04_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAQ1853152.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            complement(1741..2127)
                     /locus_tag="IXH04_002601"
     CDS             complement(1741..2127)
                     /locus_tag="IXH04_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAQ1853153.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            complement(2183..>2736)
                     /locus_tag="IXH04_002602"
     CDS             complement(2183..>2736)
                     /locus_tag="IXH04_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAQ1853154.1"
                     /translation="QALKDFGISMDKKEIYFKMKKYSTSYLINESNLNAREFNELFHR
                     YEKESTEVSRPFPETKQVLEMLKDNGGRHFILTHRLTESTWGLLKEHRLAHLIEEVVG
                     IDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDRRLDIEAGKNAGVATCLYDIDHFLG
                     EIPADYVVGNLNEILTLQSIIKK"
BASE COUNT          821 a          595 c          462 g          858 t
ORIGIN      
        1 caacctctta ttcacggtgt ttcagaagta gcttctcgga aataagccga aatttcacaa
       61 aaatttgaga aactattttt aggaaacttc ttcttatttc ttaaagctga ccacttctat
      121 cacaacctct ttttgactat gcattcacat cgatcacttt agaatcatca ttgatgacat
      181 cgcgatctaa atctttgatg cgtgcacttg tcacgatacc agctaccatg ctaccgctga
      241 cgtttactgc tgtacgtgcc atatcgatta atggttcaac agagataact aacccaacga
      301 ttgctactgg caagttcatt gcccctaaaa cgatcagtga agcaaatgtt gcgcccccac
      361 cgactccagc tacaccaaaa gaactgattg ttacaattgc cacaagcatc aaaataaatt
      421 ccaaactaaa gacattcact ccagctgctg gtgcgacgat cgttgccaac atagctggat
      481 aaacgccagc acagccgttt tgtccaatag acaaaccaaa acttgctgag aaattagctg
      541 ttgcatcatc tactcctaaa gcttttgtct gagtctcgat attcaatggc aaagctccag
      601 cacttgaacg tgaagtaaac gcaaagctca ataccggaaa ggcttttttg aaatagttga
      661 ttggattgac ttttgcacca atcaaaatca gtgagtggac aatcaaaaca gcaagcaatg
      721 ctacgtaaga agcaacgaca aatttaccta aattgacgat agctgcaaaa tcacttgttg
      781 ccaaaacgtt tgtcatcaaa gcaaatactc cataaggtgt caaacgcaaa acaagcgtca
      841 cgatccgcat aatgatttta tacagactgt cgactaaatc tgaaaagaat gctgcttctt
      901 ttggcgcttt tcttttcacg cctagataag cgattccgac aaatgccgcg aaaataacga
      961 ctccaatcgt acttgttgca cgactacctg ctaaatcagc aaaaacgttt ttagggataa
     1021 aggataaaat ctgctgtgga atcgataagt tttcaactgt ttcctgacgt gttgccagtt
     1081 cagagattcg tgctgtttct gcagctcctt gtgtgaattt agctccttgg agtccgaaca
     1141 gcattgttga accaattccg atcaatgctg caatagctgt tgttccaagt aaagaagtca
     1201 atacagtcgc actaattttc cctagctttt cagaaacttt catttttgtg aatgcaccaa
     1261 tgatcgaaat aaacactaaa ggcataacta gcatttgcaa gaatgataca tatccttgac
     1321 ctacaatccc gatccattcc attgattgag taacgacttt gtcgtctaca ccaaagatca
     1381 attgaagcag accgccaaag acgataccaa ctcctaatgc tgtaaagacg cgtgttgaaa
     1441 attttgtatg gcgcttttgc atacgataga acacatataa taatgccgca aaaactagaa
     1501 ccactaaaac tgtgatgagt gttgtcattt ttctcctcct catattatta atgacaagtc
     1561 catcgaagga agtaaaggtt cccgagaatg attcttcaac catcctcctg aaaaaatttt
     1621 tttcagtgac ttttatattt tagacgattt ttatcgaaat gacattcttt ttgcttacaa
     1681 ttagtaataa aaaaacatac ctgtgatagc tttaatctat cgcaggtatg tttttactgt
     1741 ctattcttgt ttttctgatt gaaaattcaa ttttacccca ggctgatcta aggcgatctc
     1801 tgtcacctca taagacattc ggcgtacgat atcgaataac ggtgctacta gctcgtccac
     1861 ttcttcttgt gtgacatcat tttgaccttc aattttccga ttgaggatat gattgatctg
     1921 actcacactt ccagataaaa catatgtatc aaacactaaa cggaactcta agcgtgctcc
     1981 tacgatgcta tgttctttcg gatagttttc gatcgtttct tttaaaggcg agaagcctac
     2041 tttcaaatct gtttctactt cttggttcgg caatctcata tcataatgaa atgcttctac
     2101 aacttctttt tgtcttctga tttccatata attttatcac tcctttatga aaattataac
     2161 atatctcctt tgaaagggga tactactttt taatgatgct ctgcaaggta aggatttcat
     2221 tcagatttcc taccacataa tctgccggga tctcacccag aaaatgatcg atgtcgtaaa
     2281 gacaagtagc gactcctgca tttttccctg cttcgatatc taaacgacga tcgccgatca
     2341 tcatcgtgtc tgttctttct aaatgaaaag tatcgatcaa ataatttaaa gatgctggat
     2401 caggtttacg aggaaagtcc tgatcgatcc ctactacttc ctcaatcaga tgtgccaatc
     2461 gatgttcttt taacaggccc catgttgatt ctgtcaaacg gtgggtcagg atgaaatgac
     2521 gaccgccgtt gtcttttagc atttccagta cttgtttcgt ttctggaaat ggacgggaaa
     2581 cttccgtact ttctttttca tatcggtgaa ataattcatt aaactctctg gcattcaagt
     2641 ttgattcatt gatcaaataa ctagtagaat actttttcat tttgaagtag atttcttttt
     2701 tgtccatact gataccaaaa tcttttagtg cttgcc
//
LOCUS       DACEXQ010000267         2732 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.267, whole genome shotgun sequence.
ACCESSION   DACEXQ010000267 DACEXQ010000000
VERSION     DACEXQ010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.267"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(60..1673)
                     /locus_tag="IXH04_002603"
     CDS             complement(60..1673)
                     /locus_tag="IXH04_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAQ1853155.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(1814..2722)
                     /locus_tag="IXH04_002604"
     CDS             complement(1814..2722)
                     /locus_tag="IXH04_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1853156.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
BASE COUNT          844 a          481 c          496 g          911 t
ORIGIN      
        1 tttttactcc tcaagtaaaa ataactccta gaccgcggct acccaccgcg gtcgtttttt
       61 tattcttcaa tcaaaatcgc tttgataccc aaacctgcca agattcgggc agaagtataa
      121 gtattcaatc ctcttctgaa agtaatgtaa acttttttat cttttgggat ttcattgatt
      181 cgatctcgta actcattcat tggaatattt tttgttgccg agatactgcc agaaggtgct
      241 ttattaggtt cacgtatatc taaaaagaac gcttctttca aatcgttttc gggtatatca
      301 cttgctttga tcgtttctac gatattcgtc atttgattga ttgcgacata accagctata
      361 ttcaacgggt cacgggtagt agaatacggt ggagagtaag gcaactccaa atcaggcaaa
      421 tcaaagactg tcaagtttcc ggtgattgct acagaaagtt ctcccatccg tttatcgaca
      481 cctttttctc caaatgcttg tccacctaaa atacgaccgc tatctgcatc aaaaatcagc
      541 tttagattta atcttgtagc ttctggatag aaataggcat ggtcaaaggg agtgataaaa
      601 acagttttat aatttgttat gcctgatgct tttaattcat gttctgtcat tcccgcataa
      661 cttgctgtat gatcaaagat ttttgcgaca cctgcaccaa tatatccgcg atagcgcata
      721 ggcgtaccat ttaaaatatc tgccagcata tgtccttgac gattggccgc actggaaagc
      781 atactttgga tcggctgacc agtcactaca ctagtcgttt cgataatatc gccaatcgca
      841 tagatgtccg gcagattggt ttgtaactgg tcatcaacaa tgatctgacc tgtatctgat
      901 aattgtatgc cggttgcttt caccacttca ttcttcggag aaacaccaac agcaaaaatt
      961 agcatatcag cagaaaggac ggagccatct gataatacta tttctcgacc tttatcacgg
     1021 atctcagtaa ctcttgtttc taaatgaacg gctaatcctt ctttcagcaa tttgtcataa
     1081 actaaattag caatttctgg atcataaggg aaagcgactt gtggcaattg atcgattaaa
     1141 gtcacgtcta agccgcgatg cttgaaattc tcagctaatt ccagacccat aacacctgca
     1201 ccaagaatga cgacatgttg tggatttttt tcttcaagga acgatttgat tcgatccgca
     1261 tccgtcacac tacggaggac aaagccgttt tctgcttcaa aaactccggg aatatccgga
     1321 taatctggtc tagcaccaga agagatgatc aacttatcgt aatctgtttt tatttcttca
     1381 tttgtggata ggtctttaac tgttaatcgc ttagtagaag gatcgattgc tgttacttcg
     1441 tgtttagtaa agacatcgat gttgtttttt gttttcaata tttctggtgt acgttcgatc
     1501 agcgagtcac ggtcagtgat cacacctcct aaataataag gcaaagcaca acttgcataa
     1561 gaaatatttt ctccgcgttc atagataatg atttcgtgcg cttcattcaa tcgacggaaa
     1621 cgagtggcaa atgaaggacc gccagctaca ccgccaatga caacaacttt cataaaaaca
     1681 tctccttttc tacgttgatc gtactatgtt atggtaaaaa acagcaaata attgtgccgg
     1741 aaaattattg tttattttga atagacagaa aatatagaga aataatttgt ggataaaacc
     1801 taaaaatttt tttctatttt aaggatagat aatactttga aggggataac cctgtatatt
     1861 ttttaaaagc tgaagaaaaa tgatggatcg aatgaaatcc taatatatct gcaatttctg
     1921 tataggttaa ttcattttct agcacgagac gttttgcttt ttccattttt agttctttaa
     1981 aatagctaat aacacttttt ccattcactt ttctaaaatg tttttttaag ctggctggac
     2041 tgataaaaaa aatttgggct aattgttcaa gcaaaaaatt ttctgaaaga tgttcttcta
     2101 aatatcgtat gattggatca actaattcat ctgaataaag aatatgcttt tttttaggaa
     2161 tgactttctc atttgaaggt attgtcaata aagcaagttc ctgttgataa tgccttggta
     2221 aggaagacaa ttgaattttt tgcagacaat aagtaactaa taaggaaagc aaatgaatgc
     2281 tcatctgttg gcatagtatg aaaaaagaat tgatttcctg atcataggaa atagctggct
     2341 tactatgttt tagataatca attttgaact gttcattttt cagaaaagta atgatagatg
     2401 ttatttcttg atctatagaa aaagattgtc ccgtaatatg tgctaataat tgctcatcaa
     2461 aataaaactg gataatgata aaagtttgtg tttccttttg agtagaaaag atttggactt
     2521 cttgctgttt atcaatgaat aaaagggaag gattatctag ttctatagat ttatgtgcaa
     2581 attcagcata taaattagcg ctttcattta ttagtagtcg aaaataggga gcagcagata
     2641 aatgaagatg ggtatctatg gaataggtta gtgttttttt gacaagattg gaggattggt
     2701 tgtttgttaa caaaagaacc actttccttt ct
//
LOCUS       DACEXQ010000268         2720 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.268, whole genome shotgun sequence.
ACCESSION   DACEXQ010000268 DACEXQ010000000
VERSION     DACEXQ010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.268"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..247
                     /locus_tag="IXH04_002605"
     CDS             <1..247
                     /locus_tag="IXH04_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002384409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HAQ1853157.1"
                     /translation="IEALYPLIEFNIRNIKEKGIIESLTGPVERCDISTIKGHCEVLS
                     DEDRALYMLLSKNVLEIAMLKNINRDYSELEKYLGES"
     gene            259..1086
                     /gene="panB"
                     /locus_tag="IXH04_002606"
     CDS             259..1086
                     /gene="panB"
                     /locus_tag="IXH04_002606"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HAQ1853158.1"
                     /translation="MKNTAVTFKESKLRNEKLTMLTAYDYSTAKIIDEAGINGILVGD
                     SLGMVCLGHEDTLSVTMEDMIHHTRAVTRGAKNTLVVADMPFMSYQTSVYDSVVNAGR
                     LIKEGRAQVVKLEGGIEVCDKIEAIVKASIPVMAHIGLTPQSVNAFGGFKVQGKDKEA
                     AKELIRAAKAVEKAGAFAVVLECVPTKLAELISKEISIPTIGIGAGAGCDGQILVYQD
                     MLGMYSDFTPKFVKKYANLSEEMNKAFTKYIEEVKDGVFPGPEHGFKISDDVLEKLY"
     gene            1101..1949
                     /locus_tag="IXH04_002607"
     CDS             1101..1949
                     /locus_tag="IXH04_002607"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HAQ1853159.1"
                     /translation="MVIFKTVNDVRSQVKEWKKQGLKVGLVPTMGYLHEGHESLIRKA
                     SKENDKVVVSIFVNPTQFGKNEDLGSYPRDLERDIEVCTRGRATAIFNPEVEEMYCDN
                     ASTFVNITGLTEGLCGASRPIHFRGVCTVVSKLFNIIPADRAYFGEKDAQQLAVIKRM
                     VRDLNIDIDVVGCPIIREEDGLAKSSRNTYLSLEERSSATILNKSLTLAKEALNNGER
                     DSLKIIEIISKNINTYNLAKIDYVEVVDSLSLQRVNYIEKSVLVAIAVFIGKTRLIDN
                     FTFELQ"
     gene            1959..2354
                     /locus_tag="IXH04_002608"
     CDS             1959..2354
                     /locus_tag="IXH04_002608"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HAQ1853160.1"
                     /translation="MKVTMLKGKIHRATVEQAELDYVGSITVDMNLLEAAGIYEYEKV
                     QIVDVNNGNRFETYTIAGERGTGMICLNGAAARCVSTGDKIILMAYCELNENEVKDHR
                     PKVVFVDDNNKVERVTSYEKHGRLSDIIL"
     gene            2556..>2720
                     /locus_tag="IXH04_002609"
     CDS             2556..>2720
                     /locus_tag="IXH04_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1853161.1"
                     /translation="MSRRQRRTYSKEFKQQIVNLYLAGKPRAEIIREYELTPSSFDKW
                     MKQAQSTGSFK"
BASE COUNT         1072 a          281 c          565 g          802 t
ORIGIN      
        1 aattgaagct ttatatccat taattgaatt taatattaga aatataaaag aaaaaggaat
       61 tatagaaagt ttgacagggc cagttgaacg ttgtgatatt tcaacaatta aaggtcactg
      121 tgaagtttta tcagatgaag acagagcttt atatatgcta ttatcaaaaa atgtattaga
      181 gatagctatg ttaaaaaata ttaatagaga ttatagtgaa ttagaaaagt atttaggaga
      241 gagttaggag agaaatagat gaaaaataca gcagttacat ttaaagaatc taaattaaga
      301 aatgaaaaat taacaatgct tacagcttat gactattcaa cagcaaaaat aattgatgaa
      361 gctgggataa atggaatatt agtaggagat tctcttggta tggtatgtct aggacatgaa
      421 gatacacttt cagtaacaat ggaagacatg attcatcata ctagagcagt tacaagagga
      481 gctaagaaca ctttagttgt tgcagatatg ccatttatgt cttatcaaac ttcagtatat
      541 gattcagtag ttaatgcagg aagattaata aaagaaggac gagctcaggt tgttaaatta
      601 gaaggaggga tagaagtatg cgataaaata gaggctatag taaaggcttc tatacctgtt
      661 atggcgcata taggtttaac acctcaatct gttaatgcat ttggaggatt taaggtacaa
      721 ggaaaggaca aggaagctgc taaagaatta ataagagctg ctaaagctgt agaaaaagca
      781 ggagcattcg ctgtagtgtt agaatgtgtt ccaactaagc ttgcagagtt aatttctaaa
      841 gagatatcaa tacctacaat tggtattgga gcaggtgcag ggtgtgatgg acaaattctt
      901 gtatatcaag atatgttggg gatgtatagt gattttaccc ctaaatttgt gaagaaatat
      961 gctaatttat cagaagagat gaataaagca tttacaaaat atatagagga agtaaaagat
     1021 ggagtttttc caggtccaga acatgggttt aaaataagtg atgatgtttt agaaaaatta
     1081 tattaaaagg gtcaagaaat atggttatat ttaaaacagt gaatgatgtt agaagtcaag
     1141 taaaagagtg gaaaaaacaa ggcttaaagg taggattagt tcctactatg ggatatttac
     1201 atgaaggtca tgaaagttta attagaaaag catcaaaaga aaatgataaa gttgttgtaa
     1261 gtatctttgt aaatccaact cagtttggga aaaatgaaga tttaggtagt tatccaagag
     1321 acttagaaag agatatagaa gtatgcacaa gaggtagagc aacagcaata tttaatcctg
     1381 aagttgaaga gatgtattgt gacaatgcaa gtacttttgt taatataact ggattaacag
     1441 aaggtttatg tggagctagt aggccaatac attttagagg ggtttgcact gttgtaagta
     1501 aattatttaa cataatacca gcagatagag cttattttgg agaaaaggat gcacaacagt
     1561 tagctgtaat taaaagaatg gttagggacc ttaatattga tatagacgtt gtagggtgtc
     1621 caattataag ggaagaggat ggactagcta aaagttctag aaacacatac ttaagtttag
     1681 aggaaagatc ttcagctact attttaaata aatctttaac tttagctaag gaagctttaa
     1741 ataatggaga gagagacagc ttaaaaataa ttgagataat aagtaaaaat ataaatactt
     1801 ataatttggc aaagattgat tatgtagaag ttgttgattc tttatcatta caaagagtaa
     1861 attatattga aaaatcagtt ttagttgcaa tagcagtatt tataggaaaa acaagattaa
     1921 tagataattt tacatttgaa ttacaatagg gggctataat gaaagttaca atgttaaaag
     1981 gaaaaataca tagagctaca gttgaacaag ctgagttaga ttatgtagga agtattactg
     2041 ttgatatgaa tttattagaa gcagcaggaa tatatgaata tgaaaaagta caaatagttg
     2101 atgttaataa tggaaataga tttgaaactt atacaatagc tggagagaga ggaacaggga
     2161 tgatttgctt gaatggagct gcagctagat gtgtatcaac tggagataaa ataattttaa
     2221 tggcatattg tgaattaaat gaaaatgagg ttaaggatca tagacctaag gttgtttttg
     2281 tcgatgataa taataaagtt gaaagagtaa catcatatga aaaacatgga aggttatcag
     2341 atataattct ttaggttttg taatgttatt tatatatagc tagactatta ttgaaatatt
     2401 tattgattac taattggata gatgtcaata aagtagacac aaaaagagaa gatacagact
     2461 gtagacaaag tccattttat gtgaatcggc tacactagac tagacagaaa aaataaggtg
     2521 tgtagaataa taaaaaacac actggaggat ttttcatgtc aagacgtcaa cgaagaacct
     2581 attcaaaaga attcaaacaa caaatcgtca atctctatct cgctggtaag cctcgcgcag
     2641 aaattattcg agagtatgag cttacgcctt cttctttcga taaatggatg aagcaagcac
     2701 aatcaacggg ctcattcaaa
//
LOCUS       DACEXQ010000269         2655 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.269, whole genome shotgun sequence.
ACCESSION   DACEXQ010000269 DACEXQ010000000
VERSION     DACEXQ010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.269"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(58..300)
                     /locus_tag="IXH04_002610"
     CDS             complement(58..300)
                     /locus_tag="IXH04_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853162.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKY"
     gene            complement(485..982)
                     /locus_tag="IXH04_002611"
     CDS             complement(485..982)
                     /locus_tag="IXH04_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAQ1853163.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(1112..1822)
                     /gene="budA"
                     /locus_tag="IXH04_002612"
     CDS             complement(1112..1822)
                     /gene="budA"
                     /locus_tag="IXH04_002612"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAQ1853164.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(1835..>2655)
                     /locus_tag="IXH04_002613"
     CDS             complement(1835..>2655)
                     /locus_tag="IXH04_002613"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="catalyzes the formation of 2-acetolactate from
                     pyruvate in stationary phase; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAQ1853165.1"
                     /translation="YEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDL
                     LTGSLEPQQVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMT
                     VTVDVGSHYIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGD
                     GGFLFSAQDLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYA
                     EAFGAKGIRATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
BASE COUNT          757 a          569 c          405 g          924 t
ORIGIN      
        1 cttggtcttc tcgcttttga aggcacttta tttaaatctt tatgaaacta aagtatgtta
       61 atatttgttg ctatagttat atagatattc tgttgtttcc aactcataaa cgtgagtata
      121 ttttgccact acttttccgc atttttctaa tatttcattg cgatcgacgt catgatattc
      181 gtctacgcag atcacaacac aattttctaa tttactgatt acttctccaa ccactttttg
      241 tttcaagcca ataggttggc aaatatattt ttttcctaaa atgatagttt cgttattcat
      301 atttcatcca tccttaatgt ttttccatta tttaaaatga taataccatt aaaatatgat
      361 tatgaaaatc aataacactt gtgattttat aaaaaaataa aaaaaccgaa aaacagaaat
      421 gcccactgtc tttccggctc tttttattct tgatttctct tcttttttgt ttcaatagac
      481 gattttagtt attaataaat gaaaaagcat ttatattaaa atttgctaat aaaagatcta
      541 catatttttc tagaaatgct tgatcgattt catcataccc tttcgttact ccgcaatcga
      601 tatctaaaac accgattaaa ttaccttctt ttaccattgg cactacgatt tctgattgtg
      661 cggctgaatc gcaggaaatg taatttgcat gggttttgac atcatcaacg atcaatgttt
      721 cttgtttttc agctgattct ccacaaactc cttttcccat cttgatacgt gtgcaagaaa
      781 ctcgcccttg gaacggtcct aaaatcaatt cattaccatc gaataaataa tatcctgcaa
      841 agacagtctc aggcaaagtc tcattcaaca atgcagatga atttgctaaa tttgctaacc
      901 agttatcttc tgcttctaat aatccttgtt gctgtaataa taaaatttcg tatgcatctt
      961 ttttctgttt ttctgtccac atgataaaaa ccgctccttt ttgatctatg gagaagatta
     1021 tagctcagtt tttttgtaaa cgctaggttt cctcctcaaa aaaacgagat acgtcaggtt
     1081 aatcaccttg aacgcatctc gctgtctttt tctattctgc ttgttggatt tctgctgcta
     1141 aatcagcata atcaatcgtt tcgtgaacaa aagtcggatc atcaactgga aaatgctgta
     1201 caagggacga gatgcggcta atctcaactt ttccttcagc cacttcaaaa tccattacat
     1261 gtccgccaaa tgtatgtgta tcatcgataa aatgcaagtg aaatcccgct gcagcaactc
     1321 cttggaataa ttcaggcgta aagaacccga ctaatgttcc ttggacattg ctttgttgga
     1381 attctggttg agactctgaa atttttgcta gtcttctgta aggtttttct tgcttcggca
     1441 tgatacggat gtgcatattt ttgaataatc cagttatctt cactgccaaa aataggtttt
     1501 ccccggcttc tcttactaat atgtcttttt tcaattgttc tccatctgca gtttttgaga
     1561 cagaaaaact agtatctggc gtaaaatctg taattgctgc atagggggta agttcacttc
     1621 catctaattg ggcaactgag ccatctgatc ttccttgata agctacgcca tctaaaaaga
     1681 tgacttcacc atcaagccca tgcaatgttc ccaatcccag agttcctttt tctaatatat
     1741 gagcaatcgt ttctgttcca tccatcaagc cagccatcaa agctcctaat gtcccgtgct
     1801 ggtataagat tttttcagtc atgtcgacac ttccttaata gaattgatct ggtaagattg
     1861 tttctcccaa tttctcatta tcacggtaat caatcggaat atcaataatt acgggtcctt
     1921 ctgtcgcaaa tccttcttct aaagcttttt ctaattcctc tacacttgta gctcggattc
     1981 ctttagcgcc aaaagcttct gcatatttca caaaatcaac tggaccaaag tctacacctg
     2041 acgcccgctg atatttcatt ttttcttgga actctaccat attgtagtgt ccatcattcc
     2101 aaatcaaatg aacgatattc aattttttac gtaccgccgt ttctaaatcc tgggcagaga
     2161 ataaaaatcc cccatcgccg gaaactgata cgatttgtgt atttggacgc accaatgcag
     2221 cagaaatcgc ccatggcaat gcaacaccaa gcgtttgcat accattactg aataacaaat
     2281 gacgaggttc ataacttcta aagtggcgtg ccatccaaat ataatgacta cctacatcta
     2341 cggtaacagt catatcatct gttacttttg actgaagcgt gttaataact tctaaaggat
     2401 gcaagatacc tgcttctcct tttgattgaa caatgtcccg ctccgttaat tctgcttgaa
     2461 gagatgctaa atattctttt gcatcttctg acacttgttg aggctctaat gaacctgtca
     2521 ataaatcaag agtggctgag atatcaccga tcaattctct ttcaggctgc ataaaaggat
     2581 cgatttcagc tggcgcttca tcgatcacaa tgatccgcgc atctttttcc gcattccagt
     2641 ttcttgcttc atatt
//
LOCUS       DACEXQ010000270         2650 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.270, whole genome shotgun sequence.
ACCESSION   DACEXQ010000270 DACEXQ010000000
VERSION     DACEXQ010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.270"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            155..2344
                     /locus_tag="IXH04_002614"
     CDS             155..2344
                     /locus_tag="IXH04_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1853166.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYKETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            2341..>2650
                     /locus_tag="IXH04_002615"
     CDS             2341..>2650
                     /locus_tag="IXH04_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HAQ1853167.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     F"
BASE COUNT         1002 a          358 c          441 g          849 t
ORIGIN      
        1 agccaactga ttgagaatta gaaacaatct gcagtaggga agaatcattt acacaaaatt
       61 attgacgctc ccttataatg agaactttta ctaacataat ttatgataaa cttactatat
      121 aatcatatta attaaatagt taggagatct gcttatgttt atatctactc ttcaaattcg
      181 aaattttaga aattatgaat ccgaaatctt tcgattcgat aatgagacaa atactattat
      241 aggtgagaat gattcaggga aaaccaatgc tcttactgca ttgagaatct tattagatga
      301 tagttattac tatagtagta aaacactaaa agaatcagat ttttttcatg gaattcaaaa
      361 tgggtggcaa gggcattgga ttattatctc tgcgacattt gaaggaatca gcgaagaaga
      421 atttgacaat gagatttgtg cttccatttc tttaaattct gaaagtcaaa caatcttaga
      481 agaattgatt tcaaatgcag ataaaggtgt aggtagtatt tctctattta ttagacctaa
      541 caaagctatt agaaagcaat tatttgatat ttccactgaa ttagactctc atcaatttaa
      601 cgaatttaga aattctattc gtctatccga ctatgaattt tactacacat ctaaatcaaa
      661 tctaaatttc tgtatcaatg aaaattatga taaattggta ggaagactca atgacaatat
      721 agctagtaat cctgaagaag acgatgaagc tctgttgggc tcaagaattg acatgtcaga
      781 tattttcaag catatttcgg ttgtttatgt tgacgctctt cgagatgttc ttagagaaat
      841 gaaaaacaat cgaaacccag tcaaaagaat catggaaaca attgaatcaa aaatatcatc
      901 agataatgtt gagtcattaa aaacaattat acagcaatta aatgagacta ttaccagtgt
      961 tcctgaaatt aggaaaattg gcgaaaatat taatcggcaa ctaaatagca taattggttc
     1021 agtgtattct cctaatcttc ttctttcctc aactatgtca gatgatatgg gttctttagc
     1081 aaaatttctt tcaatgaagc cagaacaaaa tatcgactta gacttactag ggttaggaca
     1141 tctaaatatg atttatttag ctttgaaaat agtcgaattt gaagcgtgtc gttcaagaga
     1201 actacttaat attatgttga ttgaagaacc agaagctcat attcatcatc atattcaaaa
     1261 aactctgttc gaaggattaa atcttcaaaa aaactatact cagattctca tgaccacaca
     1321 ttctgtacat ttagctgaag tgtctgaaat atctcggatg aatgtattaa aatcgtttaa
     1381 tggcaaatct atcgttatga atccagcaaa taatctgaac tcatttgcta aagaaaagtt
     1441 aaaaaagaac aatctaaatc taataaacgc agtagagaga tatttagatg ttaaaagaaa
     1501 tggattgctt ttttctaaag gtgttatctt agttgaagga gatgctgaag aaatacttat
     1561 tcctcaaata gtaaaaaaat tatttggtat aaacttagac gaacttggaa taggtttgat
     1621 taatgttggt agtacttcgt ttgaatatat cgctagcttg tttgacgatg atagaataca
     1681 gagaaagtgt gctattgtaa ctgatttaga tgaacaggca atcccatcag aacatcgatt
     1741 gtataaaagc actgcacaag agaaaggtca agcaagaaaa gaaaaattat cctccctttt
     1801 taatgaaaac aattgggtta attgttttta tgctaataca acatttgaaa ttgagttttt
     1861 agatgtgaat aaagaaaata ttctaactta tgtcagtcca attatagatg acgcattcaa
     1921 agataaaaaa acaataactg cttataaaga agaaatacaa agtgaacaat atttagaaag
     1981 aaatttagcg atgttaaaac tagccaatca tgttggtaag gggtggtttg caattgagtt
     2041 aggtgaaaaa attgactttt taatcaaaat tcctaagtat attcttaatg ctattgcttt
     2101 tgcatgtcag gaaatagtag atctctcaat ttataaaaaa attctactcc atacaatcga
     2161 cttgtacaag gaaactacag aattgtcagc tttatcagct agtttaaaaa caagtgatac
     2221 agctgatttg aatatagcaa taatttcttt attagagtac tttggtaaag atgaggatgt
     2281 tgtgtatcta atcaatcaga tagaaaatta cacaaaaata ctttatgctg aggtttcaaa
     2341 atgacaaata tcttcgaaaa aatcgtaaat gatttaaatt tagaacaaaa agatgttgtg
     2401 gaaagtgaag ataaccttta tgtaaatgca tgtcctggga gtgggaaaac aagggtactt
     2461 acaaggaaaa ttgcttacca agcaattaaa cacgatggat cactaaaaag aataattgct
     2521 atcacataca ctaatagagc tgctgaagaa attaaggaac gcctaagttt acttagtata
     2581 gatgatgata ttaatatttg ggtggggaca atacatcagt tttgtcttga atttattatt
     2641 tatcctttta
//
LOCUS       DACEXQ010000271         2646 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.271, whole genome shotgun sequence.
ACCESSION   DACEXQ010000271 DACEXQ010000000
VERSION     DACEXQ010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.271"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            724..2550
                     /gene="ltrA"
                     /locus_tag="IXH04_002616"
     CDS             724..2550
                     /gene="ltrA"
                     /locus_tag="IXH04_002616"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ1853168.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
BASE COUNT          953 a          461 c          585 g          647 t
ORIGIN      
        1 aaaagagtag gcacagcact tcattaaatc aatatttaat gtgtgttgga gaactaaaca
       61 cacgaatagt cgaatgacgg ggtaacgccc tgaaaggcta ctttaatact ctgactggca
      121 gaaacttgag taaacaaggc tctgtcagaa gctcggtgaa gtcggcagac agacaccgta
      181 acagataatg ctgacgaaag attcccagag gtgggcgttg tgtcctatat gccggaagtc
      241 tataaaatat ctatggttag aatgtagatg agaggtctac tgacgaatcc ttgaatgtac
      301 aggtctatat cggggctgga tagaaatgtc cagtgacaca aacagtgtcg ctactgtggg
      361 tgagtaagac tccgcattta tgaaaatcca tataacgtta aaggcgttat caaggtagca
      421 ggcttataga ggaaacctaa ggatatatgt acagatagtg tgtttggaac gttgaaagct
      481 gacaacatgg agattttact gtctgtgaag tgttcacatc agaaagttat tgtgagatta
      541 gcaatgatat aaggtgtgtt aatgtcagtg agagtggtgg cacagtacct atgaaaccgt
      601 gataacaagc ggtggaggga tagccactag tcatatgaag aatagaatta ctaagaaaaa
      661 tggtatgggt tcgagtaaga ctaagagatg tacttgctcc tgcaaacaag gagggtacag
      721 accatgctaa agaaaaacaa gctccgctat aacgagtatt atgatatgca acatatttat
      781 gacgagttat acgcacagag caagaacggt aataactttt ataagttact tgaaattatt
      841 ggttcggaac aaaatatttg tttagcttac cgtaatctga aatcaaacag tggcagtaaa
      901 acggctggaa cggatggaat gacgatagac gatataaaac aactcagcaa tgctgagatt
      961 gttgctaccg ttcgggaaag ccttagcaac taccgcccca aatcagtcag acgtgttttc
     1021 attccaaaag ctgggagcga taaaatgcgt ccattgggga ttccgtgtat ctgggacagg
     1081 ctggtacaac aatgtatcct gcaagtccta gagccgatat gcgaaccaaa gttccataat
     1141 cattcctatg gattccgtgc gaatcgaagt gcccaccatg cagtcagcag agtaacgaca
     1201 ctgataaatt tatcaaaata tcattactgt gttgacgtag acattaaagg atttttcgat
     1261 aatgtcaatc atggaaaact attaaaacaa atctggacac ttggtattcg ggataaaaga
     1321 ttgatttgca ttatcagcaa aatgctgaaa gccgaaattg acggcgaggg tgtcccagaa
     1381 aaaggaacgc cacaaggcgg tctgttaagt ccactgctct ccctcatagt ccttaatgaa
     1441 ttagactggt gggtaagcag tcagtgggaa acattccagc cgaagaaccg cagtaaaaat
     1501 gggtggcttc aatacgcaaa gaaatatacc aaactgaaaa gcggttttat tgtacgttac
     1561 gctgatgatt ttaaaatcat gtgttcaacc tatggagaag cacaaagatt ttaccacagt
     1621 accgtagatt ttcttaataa aaggctgaaa ttagaaatca gtccagagaa atcaaaggta
     1681 gtaaatctga agaaaaattc atcagatttt ctggggttca aaatcaaagt cattccaaaa
     1741 ggaagaacga aacacggtta tgttgccaaa acagatatga accaaaaagc attaaagaaa
     1801 gcgaaaacaa atctaaagtt aaaggtaaaa gatatagcca gacatacgac tggcttcaat
     1861 atcagcagat ataatctgac agttatcggt atgcagaatt actactgcat agcaactaac
     1921 gtatataaca acctgacaga agtaagttac gctctcttac cgaccatacg aatccgttta
     1981 aggaacattg caaaatcagt tccatttgaa agtacaagtt cagaatttca gagcaggaca
     2041 aaaggaatca gaccaaaaac aaaaatagtg atgattgcgg ataatccgct attgccgatt
     2101 caaggagtac aacacaaaaa tcctatgaat ttctcgcaag atatttgtaa ttttacaaag
     2161 caagggagaa acaaggtgca tgaggacgtt gtagttgtaa caaaagaaga aatccgagcg
     2221 ttgttggaaa atgaaaaccc tgccgatagc gtagagttta acgacaatcg tatttcggca
     2281 tatatcgcac aacaaggcaa ctgctatgta atgaatcgtc gaggaactcc gtccactctg
     2341 atatgtattc acaaatcaga caaaggagat aaccttgaca gatattctaa tctggcattt
     2401 gtggaaattc ccattgcaaa ggcaatccta acagaatcag cagacgaagc aaaatcactg
     2461 ttaaaaggtt atgttcttaa tagtcagcag aagaaaaaac tgaacagaat acggacgaat
     2521 tacggatacc agacaataac tgggaaataa cttataaatc tatttggata tgatggaacg
     2581 ccgtatgaag cgaaagtttc atgtacggtg tgaagcgggg gaaaacttgg agataacatc
     2641 aaaaag
//
LOCUS       DACEXQ010000272         2600 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.272, whole genome shotgun sequence.
ACCESSION   DACEXQ010000272 DACEXQ010000000
VERSION     DACEXQ010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.272"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..236)
                     /locus_tag="IXH04_002617"
     CDS             complement(<1..236)
                     /locus_tag="IXH04_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853169.1"
                     /translation="MDSFQRLSLVNQFELLAKFEDQNRSYYERKIEILREGYEYHYDD
                     EIWSDLSEPFPKEDSRFVLDVLNMYRDINFSKSKL"
     gene            698..2080
                     /locus_tag="IXH04_002618"
                     /pseudo
     CDS             698..2080
                     /locus_tag="IXH04_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2316..>2600)
                     /locus_tag="IXH04_002619"
     CDS             complement(2316..>2600)
                     /locus_tag="IXH04_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1853170.1"
                     /translation="EVAEDDPYWEIEASYIAQCAYNTTLMFSPDVIIFGGGVMKQRHM
                     LQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGTLGCLALAREAVLHS"
BASE COUNT          922 a          471 c          445 g          762 t
ORIGIN      
        1 agctttgatt tactaaaatt gatatctcga tacatgttca atacatctaa tacaaatcga
       61 gaatcttctt tcggaaatgg ctcagaaaga tcactccaaa tttcatcatc ataatgatac
      121 tcgtaacctt ctctcaatat ttcgattttt ctttcataat aagatctgtt ttggtcctca
      181 aatttagcca ataactcaaa ttgatttact agtgacaatc tctgaaaact gtccattaaa
      241 tgtttcttcc tatcttgttc attaacaaaa aattataagc ccttacaacg aatattatat
      301 attaaattta attgtaggtg aacacgaatt ttataaatat ttatggtgtt aatgacaaat
      361 gatatccgac tcctcttcat acgttctgga ctatttgcta atagattaaa atttcagcat
      421 ctatttctga ccataaataa atgagctgcc gaaaagctgc caaatcagac ttgaaaataa
      481 aggatcaaaa acttttaagt ttctgacccc gtaactcaca cttcaaaatc ttacttaaaa
      541 aggagtctga aagaaaaaaa gagtattcaa aatttttttc taactagcta catcacatct
      601 atgcattgac actcaaagaa ccagaataag tcatcagaaa gcaagggggt gcggacataa
      661 tcctggacga aaaaataaac ttaggaggaa tgtccgtgtg ttttcccaca aagagcgtgt
      721 aagggcaatt cagttatttt taaaatacga ttgctcttat gctgcaacca ttcgagaatt
      781 agggtatccg tcgattggtg cactacggaa atggtataaa gagtatttgg aatccggaga
      841 gctccatcag gagcaccgaa aaaaatcaaa gtattctgaa gaacaaaaac gaatagcggt
      901 gaatcattat tttgaatatg gtcaatgtta tgcacgaacc attcgcatgc ttggttatcc
      961 taatagagag tccttgcggc agcggtgtga agaacttgca ccaggagcta gaaaactccg
     1021 aaaaagtgcg gtaaagttaa cacaagatca aaaagaagct gtgttgaaaa aattctataa
     1081 accgcaaact aataggaaaa atttagccga atctggagga attagtagag ttactctata
     1141 tcaatggaaa aacacggttc ttggaaagga ttttccattg tgtatgactt taaaaaatca
     1201 ggaaaaacaa aaagagctac ttttaaagga agtagaagac ttacaaaaac aagtacacca
     1261 attacagctc gaaaaagcat tactcgaagg agcggctgaa ctgctaaaaa aagaaaaagg
     1321 cgtcaatctt ctttgtctat caaatcaaga aaaaacgata ttgattgatg ccttacgaaa
     1381 tcagttcacc ttgaaagagc ttcttcaaca gctccagttt cctaaaagca gctattttta
     1441 tcaaaaacaa gcacttgaga aaccagataa gtattacaaa gaacgtcaaa ttaattatca
     1501 cgattttcaa tcataacttc tgtgcgtatg gttaccgtag aattcaccaa gctttaaaaa
     1561 atatgggtaa aaagctttct gaaaaagttg tacgtagatt gatgactgag gaaaatctat
     1621 ttgttaaatt ttctagacga aaaaagtatt cttcgtatgc tggggaaatt tctcctgcac
     1681 accctaattt attaaatcgg aattttaaag caaagaagcc aaatgaaaaa tggttaacag
     1741 atattactga atttagaatt ctcgcaggga aaatctactt atcaccactt gtcgattgtt
     1801 acgatggtgc tattgttagt tggactattg gaacaaagcc aacttgcttc actctatgtc
     1861 taaaaagggt tattcaccag ataattcagc gtgcgaaggt ttctttggac gattaaaaaa
     1921 cgagatgttt tatcaacgtg actggaagaa tacaacaatc aatcagttta taaacaaatt
     1981 ggatgattat attcattggt ataataatca acgaattaag ttatctttag gagggctaag
     2041 ccctcttcaa tatcggaaga agcaaagata tatttgttaa aaaggtccaa aaaaaatatc
     2101 cgcaccccct tttttttgta aaaaactatt gcacaataca agtagcctac ggctatcgaa
     2161 atttccataa ctttcgagca agaacttatc tcattcaagg cttgatcttc caagtaaaac
     2221 aaaaaccagt taagcactct gcctaactgg ttatgactaa attttcagtt gtatctttct
     2281 ctaccaacac taaatgtcga agagcctttt ttcgtttatg aatgtaagac cgcttctcga
     2341 gccaatgcca aacaaccgag cgttccagcg ttgtcttcta atgctggggt cacgatatat
     2401 ttgcttaact caggtgtttc gacataccca ttgaccaatt ttgcaaatgc gtcgtggacg
     2461 ttttgcagca tatggcgttg tttcatcaca ccaccgccaa aaatgatcac atctggagaa
     2521 aacatcaagg ttgtattgta ggcacattga gcaatatagg aggcttcgat ttcccaataa
     2581 ggatcatctt ctgcaacctc
//
LOCUS       DACEXQ010000273         2570 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.275, whole genome shotgun sequence.
ACCESSION   DACEXQ010000273 DACEXQ010000000
VERSION     DACEXQ010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.275"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..177)
                     /locus_tag="IXH04_002620"
     CDS             complement(<1..177)
                     /locus_tag="IXH04_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAQ1853171.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSA"
     gene            complement(195..515)
                     /locus_tag="IXH04_002621"
     CDS             complement(195..515)
                     /locus_tag="IXH04_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1853172.1"
                     /translation="MITFERIKELAKKQGKSLNKVEEDLGYGKNVLYRLKNSNPSTER
                     LQEIADYFDVSVDYLLGREEKETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            1247..1504
                     /locus_tag="IXH04_002622"
     CDS             1247..1504
                     /locus_tag="IXH04_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HAQ1853173.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(1475..1783)
                     /locus_tag="IXH04_002623"
     CDS             complement(1475..1783)
                     /locus_tag="IXH04_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853174.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            1861..>2570
                     /locus_tag="IXH04_002624"
     CDS             1861..>2570
                     /locus_tag="IXH04_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAQ1853175.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSAKKFKRWVTSEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEG"
BASE COUNT          912 a          456 c          443 g          759 t
ORIGIN      
        1 ggcgctgaaa tgtgcaagtt catgagataa atgaaaggct tctgcagttt cgccatatag
       61 attttcattc aaaaaaatga ttctaggctt cggataatag aagccaggct ctttcatttc
      121 catgtaaact actttcaaat tgtattcgct caacatttct ttcaatttca aatacataca
      181 acccatcact ccaactattc attttcctct aaagctttag caattgcaat cgctttacgc
      241 attgtctcct tagatatttc ttttccatca aaagaaaaaa cagtatcgtt ttctgataaa
      301 tccacatgtt taggggtttc tttttcttcg cgccccagaa gatagtctac agagacatcg
      361 aaatagtcag caatttcttg caaacgttct gtagaggggt ttgagttctt aagtctatat
      421 aagacgtttt taccatagcc taggtcttct tcaactttat ttaatgattt tccctgtttt
      481 ttcgctaact cttttattct ctcaaacgtt atcatatcaa tattcctttc gttcaagaaa
      541 aaatatttaa ctttatttgt taaaattcgt tgactatttt aacaaaaggt gttaatatca
      601 ttcttgtaaa caagtttaac aactaaaaag acaacaaaaa acactattga ttaataaatg
      661 ccaaccgcca agaaagcttt taaaatcaat gtttatatgt cttatttaac tatgttctga
      721 ttttaacact gtatgttaat ttagtcaacg cagtttttta aaaagttgtt aaatttgttt
      781 acgaatataa aagaaaggag agaagaatat ggaaaaaaca gtctcggcaa aaatcgaaga
      841 tttgaaatta gacatactga aacaagcaaa agtggcgatg gaacacgcgg tagataaaga
      901 agactctgcc atggttgcag ccatagcaga gattttagct cacgtttagt catcgttttt
      961 acggtcttct ggaagctgac catagataac agcataatga atatctaaat aagcttgaac
     1021 aatttctttt ggcgaaattg attcgccttt agtagatact tgtgattcgt gatttagcca
     1081 tgcaacaact aaatccgcag cgatttcaaa cggcgttaca ctttgtgtca gttgccataa
     1141 agaatttcac aatatgtatg gtcgtggtaa taataccaaa gagcaatttg aggaatttat
     1201 ggaaattaaa aaatacaaaa aatcatttat taaaggaggc taaacaatga acaaggaaat
     1261 gagccaaaca atcaaggtac aaaaaatgat agatgatcta acacatggaa ttgatagcca
     1321 agccgataaa atcatcaagg aattgcaagg acaaaaagtt aaggatgcaa aaatgcttct
     1381 caagactatc aactttgaaa tgaatccaac aaaaagaaag ctcgccgatg tgttggaaga
     1441 aaagttggct tccgctatca acgagcaaga attactattt gaaaccgata cttttaacag
     1501 ctgatgtttt atagatgaca gtttcatccg aatctatatc atcgccgaga gaaaagaatg
     1561 ttgcttttga caaaaagccg ataagaccta caagcggtct tgatgttgca agagtagata
     1621 ccccaatatc accttgcaag gtattcacgt aaatttgaga agaatagaac attccatctt
     1681 tttcttcgat atttgacgca tcatcgtatc caacgaccaa agtatcggca aatattttga
     1741 ttttttctcc atcaagcata gtaattacaa attctggttt cataaatttt cacctcctta
     1801 acaattattt cagcctgtca cactgataag gaaattatac caaagaaagg aatgagaaat
     1861 atgaatacac cacaaatttt taatttcgag caaaacgaag ttcgaactat tttagtaaat
     1921 gatgaaccat attttgtagg aaaagatgta gcagacgttc taggatattc gaatccgcaa
     1981 aaagctattc gagatcatgt ggacttggag gataagacgc agaacgattc gttcaccgtc
     2041 aatggaacag cagttgtttt gattaacgag tcaggccttt acagtttaat cttaaaatca
     2101 aaacttccct ctgccaaaaa atttaaacgt tgggtaacga gtgaagtcct tccaacaatt
     2161 agaaaaacag gtagctattc aaacgtacct caaagttttg cacaagcatt gcgtttagca
     2221 gcggatttag aagaaaagaa ccaattactc gaacaacaaa ttgccgagta cgaaccaaag
     2281 attagctact tagatacgat tctttcatcg acagatacgg tagctacttc tcaaattgca
     2341 gctgattacg gaatgtcggc aattgctcta aacaaattgc taaacgagtt aggtgttcag
     2401 cataaagtta gcggacaatg gatactttac cgaaaacata tgaaccaagg atacacaaaa
     2461 tcgcacacaa gtgagatacc gaaagccgat ggcggcacta aagttgtaat gaataccaaa
     2521 tggacacaga aagggcgagt gtttatttac aacttattaa tcgcagaggg
//
LOCUS       DACEXQ010000274         2561 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.276, whole genome shotgun sequence.
ACCESSION   DACEXQ010000274 DACEXQ010000000
VERSION     DACEXQ010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.276"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(429..728)
                     /locus_tag="IXH04_002625"
     CDS             complement(429..728)
                     /locus_tag="IXH04_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ1853176.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            1079..1711
                     /locus_tag="IXH04_002626"
     CDS             1079..1711
                     /locus_tag="IXH04_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAQ1853177.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            1834..2472
                     /locus_tag="IXH04_002627"
     CDS             1834..2472
                     /locus_tag="IXH04_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ1853178.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT          864 a          404 c          348 g          945 t
ORIGIN      
        1 tcaattcctc ccccactcat ttccaatctt ttaactaaaa aattgatagt tattttacaa
       61 atatttataa aaaataaata tgccaaaaaa ctgtttttac aaggtttttc ctaatgtaaa
      121 ttattttttc gcatatattc attttatttt acttattgct ttcatttttt caatctatat
      181 ttccaatttt taacttacaa aaatgcaaca ttaataaata acgatagttt cataaaatac
      241 acttttaatt aacattatta gtttatacta gctgtttttg tgttaattaa aggataaaat
      301 acaatataaa gaaacatctg tttctttatt tagaataaaa aaatacagac tctgttcaaa
      361 taaatgaacc tagcctgcat tatgagattg actatacttt ttaattcatt gagatgaata
      421 actcgttttt ataaatgccc aaaagtaatc ggtgccgaag ccattcctcg acctaattct
      481 ttttcaaatt ttcctattgt tgatatctcc atataaaacg gtgatactgg tttagacaat
      541 tctcccctga ttgctaacgg acgtaaacta cgttccaaat tatttatgac tttaggataa
      601 tactgacctt tttctaagcc tgtctttgcg tctatcaaaa gtatacgttc cttttctttg
      661 atctctggat ttaaaacgag atcatataat tttagtacca tgttttctat ttcttcactt
      721 ttagacatca cgatttcctc ccaaaaatat atttcttccc actacattct ataaacaaag
      781 tatacgctac atctagattt ttttaaatca ttactggatt gttaaaacct ttcaatacac
      841 tgttatggca acgattttta ttttaaaaac attctaaatt aaatcagtaa aaatttttat
      901 tctaagattt ttattagaat tacgcctaat ttgtgtctct cagggcaatt ctaagaacgt
      961 ttcaagagat agcgacacta tcgttatcga aaaaggcgaa aacctatctc attttctgct
     1021 aatgtaagtt ggtttagctt ttgttaatat gactttatga aaatggaggt aattaaatat
     1081 gatttttcct aaagttaatg aagcaagctt gaataaagga tccttttttc atatctatga
     1141 aaacgaagat tttgaactgt ataatatcct ttcacaaaaa gaggtcatcg aaaaccaaat
     1201 aatctgccat ccaattattt acaaatctat tttcaagtat gcattttttg cttccaatga
     1261 ttttcaaaga atggagatta ccgactgtat attcgaaaac tgtgattttt caggctgtat
     1321 attgacaaaa agcatgttgc atcgcgtgaa atttgtcaat tgtaaattaa caggtactaa
     1381 attgatggaa agttatattg ggaacacgtt attcgagaat tgtaaaatgg actatgtaaa
     1441 tttatctggt tcaaacataa aagaaagtac ctttgagcac tctgtcttat caagcgcaga
     1501 ttttgtagac tgttccctca ctaaaataat gttttctact aatgatttag aaaatgctag
     1561 cttttttgat actgatttaa aaaatatcga tttaagtagc aactcctttg aaatgattga
     1621 agtacgctcg gaacatctaa aaaattgtac gcttaatcag ttgcaagcac ttggttttgc
     1681 tagaaaattt ttacaaataa aagtagtata atcaaaatga atatataaga taaattttta
     1741 gcaatacaaa tatccataaa aaaacattgc ctattttaac tcttcatgta tactgactct
     1801 atcttcaaaa actgaatagt aggagttatt atcatgaata acattttagg aaaaaaaata
     1861 gaacttaaaa aagggatatt gggcactatt gtcgttgtac tcggtttgtt agggttgcta
     1921 tttgcgccat ccattattag cattttttct ttaactatca ctggatttat agcatttaca
     1981 cttgtttttg gtaaaaaaga tacgaaaaaa atgttctcga aacctgtcgc tcctgtcaaa
     2041 aatatagcca aatatttttt tatcaatgta gtaatcagtg ccgcagtctc ggtatttttg
     2101 caacaaattt tgaaatgggg tttaacaggc aatccaataa atgaggcttt cagcccgtta
     2161 ttatttatta ttttgcctat catgatactt ggagaagaat tgttctcagt gtattttctc
     2221 gctatttttt ctagtaagtt cagcattcct gtcgctagtt tcttgtcagc aatcatcttt
     2281 ggatttatcc attacagtac ttatgataat ggaaatattt tacacactgt agcacacatc
     2341 cttttgatac aaggggtggc aagattgtta ttcaaccaag ctgccattaa aagcaactcg
     2401 atcattacaa gttgggcagt tcatgtgata tttgatttca cagctatatt gctagtcgtt
     2461 ttattctcct aaatttttat agaaataata catgctctat tgctaagaaa gcatacttta
     2521 tttcctttaa acaaaaagac agatgtatca atggattaaa a
//
LOCUS       DACEXQ010000275         2537 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.277, whole genome shotgun sequence.
ACCESSION   DACEXQ010000275 DACEXQ010000000
VERSION     DACEXQ010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.277"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            553..1725
                     /locus_tag="IXH04_002628"
     CDS             553..1725
                     /locus_tag="IXH04_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HAQ1853179.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDFSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFRE
                     AVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRL
                     KSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
     gene            complement(1855..>2537)
                     /locus_tag="IXH04_002629"
     CDS             complement(1855..>2537)
                     /locus_tag="IXH04_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021424053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HAQ1853180.1"
                     /translation="NVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNL
                     LKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFM
                     ERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDS
                     EEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKE
                     IYKRTYKD"
BASE COUNT          889 a          459 c          354 g          835 t
ORIGIN      
        1 acactcctct taatatacag tcctattgta taaagtgtta ctatatacat agtaactgta
       61 tacataaaaa aagtcaagta ttagaaaaaa gttcaaaaga tcaagtgtgc gcttcgctcc
      121 cactcccctc gacgttttag tagcctttcc ctcacttcgt tcagtccaag ccaactaaaa
      181 agttttcggg atacattctc cttctcttcc taataatcaa ttaaaatttt aatctttata
      241 tttctgctaa tcactcagga aacagtacag aaaaacaaac acgtatcata gatataaatg
      301 taatggcata gtgcgggttt tattttcagc ctgtatcata gctaaacaaa tcgagttgtg
      361 tgtccgtttt tgggcgttct gctagcttgt ttaaagtctc ttgaatgaat gtatgttcta
      421 agtcaaaaaa ctctgacagc gcctttatat agataaagtc cgtataattg tgtaaaagta
      481 aaaaggccat ataacagtcc ttttacggta caatgttttt aacgacaaaa acatacccag
      541 gaggactttt acatgaccca agtacatttt acactgaaaa gcgaagagat tcaaagcatt
      601 attgaatatt ctgtaaagga tgacttttct aaaaatattt taacaacggt atttaatcaa
      661 ctaatggaaa atcaacgaac agaatatatt caagcaaaag aatatgaacg aacagaaaac
      721 cgacaaagtc aacgaaatgg ctattatgag cgcagcttta cgacacgtgt aggcacgcta
      781 gaattaaaag tacccagaac acgtgatggc catttttcac ccacagtgtt tgaacgttat
      841 caacgaaacg aaaaagccct catggcttca atgttggaaa tgtatgtatc aggcgtttca
      901 actcgtaaag tatcaaaaat tgtggaagaa ctttgtggta aatccgtctc taagtccttc
      961 gtttctagct taacagaaca gctagaacct atggttaacg agtggcagaa tcgtttatta
     1021 tcagaaaaaa attatcctta cttaatgacc gatgtactct atataaaagt acgagaagaa
     1081 aatcgagtac tctcaaaaag ctgtcatata gcgattggaa taaccaaaga tggcgaccgt
     1141 gaaattatcg gcttcatgat tcaaagtggc gaaagcgaag agacctggac aacatttttt
     1201 gaatacctaa aagaacgcgg tttacaaggt acggaactcg ttatttctga tgcgcacaaa
     1261 ggattagtct ctgccattag aaaatccttc accaacgtaa gttggcaaag atgccaagtt
     1321 cacttcctaa gaaatatctt taccaccatt cctaaaaaaa attcaaaatc tttcagagaa
     1381 gctgttaaag gaatttttaa gttcacagat attaacttag cgcgtgaggc taaaaatcga
     1441 ttgattcatg attatatcga tcaaccaaaa tattcaaaag cttgcgcatc attggatgat
     1501 ggattcgaag acgcctttca atataccgta caaggaaatt cccacaatcg actaaagagt
     1561 accaatctaa ttgaacgact gaatcaagaa gtacgcagaa gagaaaagat tattcgcatc
     1621 ttccccaatc aaacatcagc caatcgctta attggagccg ttcttatgga cctacatgat
     1681 gaatggattt attcttcaag aaaatacatc aattttgata agtagaaatg gtaaaaacat
     1741 tgtatagcat tttacacagg agtctggact tgactcactt cctttattat ttttcatttt
     1801 tttaaactat ttaatactaa tgtcttttat aatagctttt catatattat ataatcaatc
     1861 tttataagtc cttttataaa tttcttttct accattttcg ataaattcct gtttaatatt
     1921 tttaattcca taaacaatag tttcaatagg ataatattct tcaactatat cttgatattc
     1981 ttttgctttc tcaatatcta tatttccata cattcttaat atatcttctc caaaatttgt
     2041 tcctatttct tcttcactat cttcaagtaa gtatataaaa tcacaatatt catctataat
     2101 tccagaatct ccaaaatcaa ttattccagt taatctatta ttgccatcta acaatagatg
     2161 attacaacta aaatcattat ggcataaaca ctttttaccc tcaaaaactg ttgttgcatt
     2221 tagtctttcc ataaaacttt ctatataatc tttttctata tcagttaaat cattataaat
     2281 agtttcacgc aacaatatat actcttctaa tacattttgt ttattatcaa tagtacattc
     2341 actaatatct gtataatcta aaccgtgcat ttgtcttaaa aaactggcaa tatctcgttt
     2401 taacaaattt tgttcttctt ctgacatagt agaataaatt tctggtgtta aaaaagttcc
     2461 tttaatttct ttataaccta gtatagataa ttcatcacta atatacgaat attcaatatt
     2521 aggaattttt acattag
//
LOCUS       DACEXQ010000276         2499 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.278, whole genome shotgun sequence.
ACCESSION   DACEXQ010000276 DACEXQ010000000
VERSION     DACEXQ010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.278"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..236
                     /locus_tag="IXH04_002630"
     CDS             <1..236
                     /locus_tag="IXH04_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853181.1"
                     /translation="MGKGSMDITVIQGAKAVYVAYRQANMVDYISFDEFIDKWDFDME
                     VAVAVYSTMMFKQARDAYQKEFEKANKEKKLQK"
     gene            239..421
                     /locus_tag="IXH04_002631"
     CDS             239..421
                     /locus_tag="IXH04_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853182.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            439..>2499
                     /locus_tag="IXH04_002632"
     CDS             439..>2499
                     /locus_tag="IXH04_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ1853183.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNDIDKTSKALAQSKGEFKTYESTMKDLDNEQQHLKSSASLVESEYKKWQ
                     ATAGQSATESEKLAKAQEYVGKQSDIAEQKIEVLKKQLDATQKEFGETSTEALEMKTK
                     LNDAEREFEELSNAAKNVDTSTVDDIGKKLDMGNLMEATDHLSVIGDKLIDVGSKSIE
                     AAGKAQAMQAQFKQVFGSLEGEAQDAVEGMAEEFGMLPNTIKPVFTQYTSMFKGLGYD
                     TKEAMELAGDSTQLAADAAAFYDKSMDDASESLNSFIKGNYEGGEQIGLFANDTQMAA
                     YAVKHNLIPATEGAKKASEESLLAVEKAQSKYADAIKKHGEGSLEAREAALKLKDAQD
                     KINEELGPQTQKWSDLDEATKQAVRVQYAEDMQKLAGATGQASRESDGLENQMTRAKQ
                     ALEDFYASLGEDILPVFIKGLQAGAKALQGLAEWWSKLDGPMKNFILALGGILALLST
                     LAPVITAVVTIVGTFGSTVLLPIIGIIAGVAAVIAIVITAFQNWGAITDWFSDLWKKF
                     TDWLGDTWESIKESASSVWDGVKETWSGFVDWVQEIWQGVSDWFGELWSGLVEGASNI
                     WQGVQ"
BASE COUNT          912 a          369 c          568 g          650 t
ORIGIN      
        1 cgatgggaaa aggctctatg gatattacag ttattcaagg tgcaaaagcc gtatatgtag
       61 cttatcgtca agcgaacatg gtcgattaca tttcattcga tgaatttata gataaatggg
      121 attttgatat ggaggttgca gtagctgtat acagtactat gatgttcaaa caagcacgtg
      181 atgcctatca aaaagaattc gaaaaagcaa ataaggaaaa aaagcttcaa aagtaaaaat
      241 gccaaagctc ttagttgaaa cgtgggtcga tgtctattcg atgttgaccg acgttttttc
      301 tatgccttca gatttggttt taagcgatat ctgtttagat gacattttgc aaatggctta
      361 caacaagagc gcttatgaag gatggaagaa ctatgcaata aatcaatccc agaaaaacta
      421 aaggaaagga ggtaaaaaat ggctaaaaag agaacggaag cagaagtaac tttcatagct
      481 aacgatgacg gattgaaatc tacgttaaaa gaaatcagtg ctgaattaac taaaaataga
      541 gcagaattaa aactagaaca agctcaatta caacagactg gttctgaatc agacaagtta
      601 ggaagtaaat tatcttcttt agaaaagcag tatgaattac aaagtcaaaa agttgaagta
      661 actagtcaac gtttagccaa tgccaaaaaa tattatggag aaaattccac cgaagttcag
      721 aaacttgaaa gagaactgat taatcaacaa acagctcagc aaaggttatc taatgatata
      781 gataaaacga gtaaggcatt ggctcaatct aaaggtgaat ttaaaacgta tgagtcaact
      841 atgaaagact tagataatga gcaacaacat ctaaagtcaa gcgcatcatt agtagaatcc
      901 gaatataaaa aatggcaagc aactgctggc cagtctgcta cagaatctga aaaattagca
      961 aaagcacaag aatatgtcgg caaacagagt gatattgctg aacaaaaaat cgaagtgcta
     1021 aaaaaacaat tagatgccac acaaaaagaa tttggagaaa catctaccga agctttagaa
     1081 atgaaaacta agcttaatga tgcggaaaga gaattcgaag aattaagtaa tgctgctaaa
     1141 aacgttgata cctccacagt agatgatatc ggcaaaaaac ttgatatggg taatttaatg
     1201 gaagctactg atcacttgtc agtgatcgga gataagctta ttgatgtagg tagtaagtct
     1261 attgaagcag ctggaaaagc acaagctatg caggcccaat ttaaacaagt ctttggctct
     1321 ttagaagggg aagcacagga cgccgttgag ggaatggctg aagaatttgg aatgttacca
     1381 aatacgatca agcctgtttt tacacaatat acgtcaatgt ttaaaggact tggatatgat
     1441 accaaagaag ctatggagtt agctggtgat agtactcagt tagcagcaga tgcagcagct
     1501 ttttacgata agtctatgga tgatgctagt gaatctctta attcatttat aaaagggaac
     1561 tacgaaggtg gggagcaaat aggtttattt gctaatgata ctcaaatggc agcttatgct
     1621 gttaagcata atttgatacc agcgactgaa ggagcaaaaa aagccagtga agagtcattg
     1681 ttagctgttg aaaaagcaca atctaagtat gctgatgcta ttaagaaaca tggtgaagga
     1741 tctttagaag caagagaggc tgctttaaaa cttaaagatg cgcaagataa aataaatgaa
     1801 gaattaggcc cacaaacgca aaaatggtct gatttagatg aggctaccaa gcaagcagtt
     1861 cgagttcaat atgctgaaga tatgcaaaaa ttagccggtg ctacaggtca ggctagtagg
     1921 gagtctgatg gtttagaaaa ccaaatgaca agagcaaagc aagcgttaga agatttttat
     1981 gcctcattgg gtgaagatat actgcctgtg tttatcaaag ggttgcaagc aggagcgaaa
     2041 gctttgcaag gattagccga atggtggagt aaacttgatg ggccaatgaa aaatttcatt
     2101 ttagctcttg gaggaattct agcattatta agcacattag ctcctgttat aaccgcagtt
     2161 gttacgatag ttggcacatt tggttctaca gttttgctgc caataatagg aattatagcg
     2221 ggcgttgcag ctgtaatagc gattgtaatt acagcgtttc aaaactgggg cgcaatcact
     2281 gactggttta gtgatttatg gaaaaaattt accgattggt taggtgatac ctgggaaagt
     2341 ataaaagaaa gtgcctcatc agtttgggat ggagtcaaag aaacctggtc tggatttgta
     2401 gattgggttc aagaaatttg gcaaggagtt tctgattggt ttggagagtt atggagcgga
     2461 ttagttgaag gagcttccaa catctggcaa ggagtccaa
//
LOCUS       DACEXQ010000277         2487 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.279, whole genome shotgun sequence.
ACCESSION   DACEXQ010000277 DACEXQ010000000
VERSION     DACEXQ010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.279"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(185..1504)
                     /locus_tag="IXH04_002633"
     CDS             complement(185..1504)
                     /locus_tag="IXH04_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HAQ1853184.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDKRAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            1634..2455
                     /locus_tag="IXH04_002634"
                     /pseudo
     CDS             1634..2455
                     /locus_tag="IXH04_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
BASE COUNT          754 a          434 c          469 g          830 t
ORIGIN      
        1 taattctttg gcacatttta ttgaaatatg tatcactaat atccctatca actgattttg
       61 actaaaaagt tacttcaaac attcactgtt caaaaatatt gaggaataaa ttttgggtgt
      121 aaggcatttt gagcatactt ctccctggat aacttcccag taaaaaaatc gttagattgt
      181 tggattagat ccattgactg gctcgcctca ggcgagcaaa gaccctgtag aattcattct
      241 gatacacatg ataactagat aatttgagat agactcgtct acccgtttgg actaatttcc
      301 cagcaacttt cagaaacttc aatcgaatag aatgaatggt cattcccttt tggacttctt
      361 caaagccaat cgttcttaag aagttgacta agttgtaagc tatcaagctg agcatcattc
      421 ggacatgatt ctccaaaaag cgaggactgt ctgttttgtc aaagtaaaag ccagctttcg
      481 cttctttaat gaagttctcc attgtgccac gtttggcata gatagaaaag atggtatcag
      541 gagaaacatt ttctgataga ttcgtcacga taaattcatg tcgaaagagt agttcgcccg
      601 cttcacgtgt tgagcgtata cacacgcgac gactttgtga ccaggattgt gcttgataag
      661 tggtcgagaa gtactgaact tctcgttctt cccacttttg attatcgcca taaagtactg
      721 atttctcagc tatttgacct agtctacgat tattcttcag tcgaataaca taatgactct
      781 tttttgattc acacgaatca tacacaccag gtgtagcgaa cccgctgtct ccacgaacca
      841 agatgtcagt gtttggtaga gagtgattat agtgctctaa taaaggtgtg agaaactcct
      901 ttacgccttt tgatgtatat tgatttcctg aacgtagttc agcttttaag aagtcaccag
      961 tcaatccgtc aaaagctacc aatggatggt aaccataggt ttggtagtgt gtattataat
     1021 tcgtttgttc ttggtgacca aatgtatctg aatgggtcga atctaaatca atgattaatt
     1081 ccgtatcatt acggatgagg cgtgctttat cgataagttc ttggttcaaa gcttgaagtt
     1141 catggatatt ctcctcagat agtcgatcta aaaatcggga aagtgaagat tgagaagcga
     1201 gttctttcct atctaaaaca gctttaaaca caggatcttg tctcaggaga ttagctgcag
     1261 aatcagctga gtaaccagca attaattgca taatgaactg ctcaagtatg gataagttgt
     1321 catgagtaaa atacgctcgt ttgtcttcaa tgtgaatgtg ttgcttagcc agttcagaaa
     1381 aaccaaagat gttcatcagc tctttcacta ggacgagacc cgaatcactc gataattgac
     1441 cacctgtatg agaaatagtg atatttgaat tgaattttac ttggtttttg tgtaagctag
     1501 tcatgaaaag aactcctttc ttttggttgg tttagtcact ttaaccatag cagaaagggg
     1561 ttcttttttc atcacttaac gggtgaagat gaaaaagtaa ttgtaagctg ccgtgaggca
     1621 gtttcacatg gttttaatta aaagtatgaa ttattcagga attaaaatgc cggcaagcgt
     1681 tccaccaaat gtaagccgag ctttcgttgc tattattcct ggcttttttg tcgttctact
     1741 atggtttatt gttgttgtaa tttgttataa acttggaatt gaaaatgttc atgctcttat
     1801 tgcagataca ctcgcaaaac cactatcttt actgacgaaa actttaccag gaattatatt
     1861 ggttatactt attcaatgtt tcttttggat gtttggtatt catggtgcac aggtaacggg
     1921 gccaataatt gaaccgttat tattgcaaaa ctctgatgta aatagaattg cttatcaagc
     1981 tggaaaagaa cttcctaaca ttataactta tgaattctta tataactttg ttttttctgg
     2041 tggtgcaggt tgtttatttg cacttgccat tttaacattt ttcttttcaa agagtcagga
     2101 aaataaaact ttaggtaagt tatcaatcgc tccagtaagt tttcaagtag cagagcccct
     2161 tctatttggg tttccaacta tattaaatat aaaaatgtta attccatttg taactgcacc
     2221 agtagtaaca acattaatta cttatttttc aatgagtatg ggattagttg caaaacctgt
     2281 aggagcaaca atcccatgga caactccacc gataattgcc ggatttttag cttctggtgg
     2341 acgaataagt ggtgcagtga ttcaagtaat aacaattgca ataaatgttt tgatatacta
     2401 tccattcttt aaattagatg ataatgccaa gctaaaaagc gaaaaaaatg attagaacta
     2461 cctatatgtt tctaactgta gttaagt
//
LOCUS       DACEXQ010000278         2476 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.280, whole genome shotgun sequence.
ACCESSION   DACEXQ010000278 DACEXQ010000000
VERSION     DACEXQ010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.280"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            234..476
                     /locus_tag="IXH04_002635"
     CDS             234..476
                     /locus_tag="IXH04_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HAQ1853185.1"
                     /translation="MENELLENLGRLHTTELGVIRIKKNLSLNVENVIEWCKEKISLS
                     NAEITRKGKNWYITIDNCIITVNAYSYTVITAHKVK"
     gene            473..943
                     /locus_tag="IXH04_002636"
     CDS             473..943
                     /locus_tag="IXH04_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1853186.1"
                     /translation="MINFSIKELNAFAVIGQEVELTNYQKKNIQISTQFWRKFNSSLK
                     KAYLSQSGNWVKYAFMERRNGKLYYFCSIPQRTITPEGFLYKEIPSYKYLVVEHIGAM
                     DKIYETYGKIYEEIIPSTSYIPIKDIILHFEKYDYRFHWNSDNSVIEIWIPIQD"
     gene            1071..2285
                     /locus_tag="IXH04_002637"
     CDS             1071..2285
                     /locus_tag="IXH04_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ1853187.1"
                     /translation="MNDKNFIEELRQKREEYGVTQTRLAVACGISREYYNRIEKGKQP
                     LNDELREIIEKQIERFNPQEPLFLLIDYFRVRFPTTDALAIIRDVLQLKSNYMLYEDY
                     GKYGYESKYVLGDINIMCSMQEHLGVLLELKGKGCRQMECYLLAQERSWYDFMLDCMT
                     AGGVMKRLDLAINDKAGILDIPKLKEKYKAGECMSYFRMQKDYSGTEKCGSDLPKNTG
                     ETLYLGSTSSELYMCAYQKNYEQYVKNGTEIEDTEIKNRFEIRMKNERAYYAVVDLLT
                     YRDAERTAFSIINHYVRFVDREDDKPKSQWITNDDWAWFVGENREPIRLTTKPEPYTL
                     QKALHWLQRQVAPTIKMVQALDRENRTTILKDMIEQAELKDKHKHLLQLEKSTIEERI
                     DTAVPQENDGIF"
BASE COUNT          893 a          354 c          505 g          724 t
ORIGIN      
        1 aagaaattga ttgtcgtttt cttttgcaga aatttaagtg caggcaatta tggtagttgt
       61 cgtaattgct gtcagctcag tgtttatcac tgatggtgtg aaagatttac ggttggagat
      121 gttcaataag attattggtg cttaattatt tttattgtag tgctgatatg agaatgttat
      181 aattggggta taaaacatac aaataaattt gctgaaaaat gaggtatcaa aatatggaaa
      241 atgaattact ggaaaactta ggaagacttc acacaactga acttggcgtt attagaatta
      301 aaaagaactt atctttgaat gtagaaaatg taattgaatg gtgcaaagaa aaaattagcc
      361 tttctaatgc ggaaatcaca agaaaaggaa aaaactggta tataacgatt gataattgta
      421 ttataacggt aaatgcgtac agttataccg ttatcactgc tcacaaggta aaatgataaa
      481 tttttctata aaggaattaa acgcatttgc agttattggg caggaagtag aactgactaa
      541 ttatcaaaaa aagaatattc agattagtac acagttttgg agaaagttta acagtagttt
      601 gaaaaaagcg tatctttcac aatcaggaaa ttgggtaaaa tatgctttta tggaaagaag
      661 aaatggaaaa ctttattatt tctgttctat tccccaaaga accattaccc cagagggctt
      721 tctgtataaa gaaataccgt cttataaata tttggttgtg gagcatattg gtgctatgga
      781 taaaatatat gaaacttatg gcaagattta tgaagaaatt attcctagta catcgtatat
      841 tcctataaaa gatattatcc tacactttga aaaatacgat tatcgttttc attggaatag
      901 tgataattca gttattgaaa tttggatacc tattcaagat tagaagcaat tttatatcca
      961 tacacacaaa gcagttagtc ttaggattga ctgctttttc tatacagatt tttaatgaca
     1021 ggcaattatc aattatggta gttgcctttt ttgattggag gaattgaaga atgaatgata
     1081 aaaactttat tgaagaacta agacaaaagc gtgaggaata cggagtaaca caaacaaggc
     1141 ttgctgttgc ctgtggtatc agccgtgaat attacaaccg cattgaaaaa ggaaaacagc
     1201 cattgaatga cgaattaaga gaaatcatag aaaaacagat tgagcgtttc aatccgcaag
     1261 aaccactatt cttattgatt gattactttc gtgtccgctt tcctactacg gacgcattgg
     1321 cgattatccg tgatgtatta caactgaaat ctaattacat gctttatgaa gattatggaa
     1381 aatacggata tgaaagcaaa tatgtacttg gcgacatcaa tatcatgtgt tccatgcagg
     1441 agcatttagg tgttttattg gaactgaaag gcaaaggctg tcggcaaatg gaatgttatc
     1501 tgctggcaca ggaacgctca tggtatgact tcatgctgga ttgtatgacg gctggtggtg
     1561 tgatgaaacg gcttgatttg gctatcaatg ataaagcagg aatattggat attccaaagt
     1621 taaaggaaaa atacaaggct ggcgaatgta tgtcctactt ccgtatgcag aaagattaca
     1681 gcggtacaga aaaatgcggt agcgatttac caaagaatac aggagaaacc ttatatctcg
     1741 gttcaacaag tagcgaatta tatatgtgtg cttatcagaa aaattatgag cagtatgtca
     1801 agaatggcac agaaattgaa gatacggaga ttaagaaccg ttttgaaata cgcatgaaga
     1861 acgaacgagc ctattatgcg gttgtagatt tactgaccta tcgggacgct gaacgcaccg
     1921 ctttttctat catcaatcat tatgtccgct ttgttgacag agaggacgac aagccaaaaa
     1981 gtcaatggat aacaaatgat gattgggcat ggtttgtagg ggaaaacaga gagccgatac
     2041 gcttaaccac aaagccagag ccttacactt tacaaaaagc gttgcattgg ctacaaagac
     2101 aagtcgcacc aaccataaaa atggtacagg cattagacag agaaaatcgt acaacgatac
     2161 tgaaagatat gattgagcag gcagaactaa aagacaagca taaacattta ttacaattag
     2221 agaaatcaac catagaagaa cgtatagata ccgctgttcc acaagagaat gacggtattt
     2281 tttaatgtaa ttgaaaaatt tcagatttag tcagcaaaga gctgttttca ttccctatgt
     2341 gaagtgaaag aagaaaaata aattttttat taaatctgca acaaaatggc tacttgcgta
     2401 cagatatggt gcgagaaaag gaaatctaaa ctttttttga aaataggtca ttaaatcggg
     2461 gcttgctgtc cctata
//
LOCUS       DACEXQ010000279         2439 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.281, whole genome shotgun sequence.
ACCESSION   DACEXQ010000279 DACEXQ010000000
VERSION     DACEXQ010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.281"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            25..402
                     /locus_tag="IXH04_002638"
     CDS             25..402
                     /locus_tag="IXH04_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853188.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            1008..1622
                     /locus_tag="IXH04_002639"
     CDS             1008..1622
                     /locus_tag="IXH04_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1853189.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
BASE COUNT          839 a          351 c          450 g          799 t
ORIGIN      
        1 tataaaaaaa ggtgtgtgtt cactatggga aaattagtcg ctgttgcaca gtcgctgctt
       61 gatatactca agtggctgat ggaaggaagc aattggatat ttgtgttgcc actattgctt
      121 attagtctat ttatcatgtt tcgcatggtc agaaaaaaag aaacgtttct gatttttatc
      181 tggtatgcag catccgttgg ggttgctacc ttgatggttg aattggcgtt aggtagttgg
      241 gaagcttgcg tatttgcttt attttttgct gtactgtatc taatcattta tatcccatat
      301 atgaaaagaa agtctcggat aggcagaaaa ggtagacata caactacttt ttctagtaca
      361 gattctctcg aacaaagtag aagaacgaga agaagacggt aatatttagt aggtatacgc
      421 aaaaaaagat aaaataatgg ataaactatt gagatgagag atttatttaa ttggtggaaa
      481 aaagcttttt tcacttatat aaaagaatct tttttaaagg aatgcttgtt gtatcgggat
      541 aagccattcc tttttttatg ttatttatat atatataaat cataccaatg tttttttgta
      601 ttttcttgct tgcacttgga taagagatta cttacaataa attcagatag aaacagctga
      661 ataaaaaaag gctccctgac ccttagttgg cgctaagaat cagggaatga ccagtcacac
      721 acatacaccc tgaccgatca ttttgccata gtcaatgcag tgcatcgacc taatttattg
      781 tacctaatct agggtgaaaa ttctacccct atgtagatat tttccatggt ctgcacgttg
      841 ttttatttgc tatgtcaagg tattgggcgt attcgttcaa tgcctttttt gtgtttgaaa
      901 cagcaaagac cacatgtgtt actgtctaac tactgttttt ttatgtactt ccttagatta
      961 ggcagctgtt tctattcatg tcaagtatag ctaggcggga gagataaatg agtttatgtt
     1021 attttgacta ttatcaccaa ttggaagaga aattaaggat agaagagccg ctgatgcttc
     1081 aagctggcac ttggatcgcg aaacaaatta atcaaggtgg tagattgcag atatatgcta
     1141 gccgcacact aagtggtgta gcatttgaat tttgggatca aaccccggat cttgtaccaa
     1201 gtatattgat tgaaaatcca gccggaggtg tctatgaaac gctagaaggt gcaggacagg
     1261 caattattga tcagctttct gtcaaatcag aagacatttt tcttcttctt tcgaatgaag
     1321 gaagaaaccc ggcaatcatc gaactagctc agtggatcaa agaaaatggt catccattaa
     1381 tcattgttac tggatttgat ttgtcacgaa gtatcaaatc gcaacatagc agtggtttgc
     1441 gattatttga atttgctgat ttggttttgg acaaccacgc caatttacat gatgcagtct
     1501 tgactttgcc aaatctagat ccagcgatct gcggaacagc ctctcttgca acgatctttt
     1561 tgctgcagca agttttgtat tttacagcaa aacagctagt ccaggacaca agtggaaaat
     1621 agttatccac atgttgacaa agttccttaa aaaaataatg ttccacataa aaatggagcg
     1681 tttgaaaaaa agaaaacgat tattaatgag gattaacaac gactactttt tttgttttag
     1741 ttattttaag atggatcatc tttgatttta agcttgtttt gtttaaagtt tgttattttt
     1801 ataagttgtt tcacagttat gagaaatgag ttataagaat atgtagtgtc agaaaaataa
     1861 aaagtagtgg aaaaagagac aagaaagaat ccatatggat tctttcttgt ctcttaatac
     1921 acgttgatga aagagtcttc cccaaaactc tttacaaagt aattatagta catttgttat
     1981 cattattgca atgaagttta cctgatataa caaaattgtc caacaggata cctttttcta
     2041 atagaaataa aatacacaaa aattgaagga tagataatgg atatttataa gtgtttatta
     2101 ttttttttgc aatgaaattc aatttttcaa tgattgtaaa atgaaaaaaa caggacgaga
     2161 ttttctctaa tctaaaaata aaaaaagaat tttaaggaaa aacagagaaa taaaatactt
     2221 aatataaatg gaaagagaaa aaaataatca tacataggag aaaaaaaaca aaatagaaaa
     2281 aagtgatttt ctaataaatt attcatgtgg aaatgtcatt tttactgtat aattcatttg
     2341 attatccatt ttttattcat caaaattact gaaaaaagaa aggaagaaat aaatttgaag
     2401 aaaacaaatt cttcaggaaa accgaaacgc gacttcact
//
LOCUS       DACEXQ010000280         2422 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.282, whole genome shotgun sequence.
ACCESSION   DACEXQ010000280 DACEXQ010000000
VERSION     DACEXQ010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.282"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..467
                     /locus_tag="IXH04_002640"
     CDS             <1..467
                     /locus_tag="IXH04_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853190.1"
                     /translation="KENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            539..934
                     /locus_tag="IXH04_002641"
     CDS             539..934
                     /locus_tag="IXH04_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1853191.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            918..>2422
                     /locus_tag="IXH04_002642"
     CDS             918..>2422
                     /locus_tag="IXH04_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HAQ1853192.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKA"
BASE COUNT          840 a          397 c          459 g          726 t
ORIGIN      
        1 taaaggaaaa cgatttttca gatatgctag ctgtctatgc taattcggta aaattatata
       61 cagacacgaa aacaataaga gaatattttg gtatcgaata cagtagtaac ttgggagatg
      121 ttttatttca atttagccaa actcttgctc gatttattcc gacagaagtt agtgagaaga
      181 aaaatgaaga ccaaaaagaa gccatgtgtt tctcattaag tcaaagtgat tcagaagatc
      241 ccagaaaaaa atattgtttt tcagttagaa gaaatccttt aaagggaaat ggtgagctgg
      301 gaaaacgaag tccttttaat gacaataaaa cgagattgta tagacccagc ttatatgaac
      361 gattagggtc agataccaat ttaagtttca gatattcaat gaatccagat gatgaagaaa
      421 ctgatgaagg tattattgca aaatggacaa aaaataaaat tgaatagtta aagcattgaa
      481 agagcaatca cctattttga ttgttctttt tctattttta cggaaaggac gtgaagacat
      541 gaaaaaaata agaagttaca ccagtatttg gtcagtagaa aaagtgattt atgcgattaa
      601 tgatttacaa ctccctttcc caattacctt tacgcaaatg acatggttta tcgtttcttt
      661 gtttgtcgtc atactctttg gcagtttacc accactttct ctgattgacg gagcactact
      721 caaatactta gggattccca ttgttctcac tgggttcatg tgccaaaaga cctttgacgg
      781 aaagaaaccc tatggatttc taaagtctat cattacctat gctctacgcc ctaaagtaac
      841 ctacgctggc aaacaaatac gattaaaaaa agaaacaatg aatgaatcga ttacgattgt
      901 aaggagtgaa caactagatg gcatatccga ttaaatatat ggaagataat ctcgtattta
      961 atcatgatgg cgaagtattc gcttattatg aattaattcc ctataattac agttttttaa
     1021 gttccgatga aaaaattcaa gttcatgata atttctgtca attgattgcc caaaatcgtg
     1081 atggcaagat tcatgctctg caactagcaa ccgaatcaag tattcgagcc acacaggaac
     1141 aatcgaaaaa agaaatcact ggacgattaa aagagatagc ttacgaaaaa atagatgaac
     1201 agaccgaagc gttaatttct atgattggtg acaatcaagt agactatcgc ttttttattg
     1261 gttttaaact ctacctcaat gaacaggaag tatccgtaaa aaatgttggg aaagaaattc
     1321 aaaaagcatt tgcggaattc tttcatggtg tgaatcacaa attaatgggc gattttgttt
     1381 ctatgcctat ggcggaaatc gaacgctttt ctaagatgga acagttgtta gaaagtaaat
     1441 tatccagacg ctttcagatt cgtaaattgg agaaagatga ttttggttat attattgaac
     1501 atctttacgg gcaaacaggt gtcccttttg atgactacga ctaccattta ccgaaacgat
     1561 acgaagataa aaaagtgttg gtaaagaaat acgatattct caaacccact cgctgtttaa
     1621 tcgaagaaaa tcaacgctat ttacggattg aacaggaaga taccactact tatgtggcgt
     1681 attttacaat taatagtatt gttggtgaat tggatttccc taatagtgaa attttttatt
     1741 accagcaaca gcagtttact ttccccgttg ataccagtat gaatgttgaa attgtcgcca
     1801 ataaaaaagc cttatctacc gttcgcaata aaaagaaaga actgaaagat ttagatagcc
     1861 acgcatggga aagcgacaac gaaaccagta ctaatgtggt ggacgcttta gaatctgtcg
     1921 atgaattaga aagtaattta gaccaaacca aagaatctat gtacaaacta agctatgtgg
     1981 tacgggtatc agcaaagaat ttggacgagc tgaaacaacg ttgtgatgaa gtaaaagatt
     2041 tttatgatga tctgtctatc aaattagtac gaccttttgg cgatatgcta ggcttgcata
     2101 atgaatttat tccctcaagc aagcgatata tcaatgacta tattcaatat gtcacgtcgg
     2161 attttttggc tggtcttggt ttcggtgcta cacaaatgat tggtgaaaat tctggtattt
     2221 acgtcggtta caatctcgat acaggcagaa atatttatct ccaacctagc cttgcaagtc
     2281 aaggaattaa aggctctgta acaaatgcat tagcgtccgc ttttattggt tcattaggtg
     2341 gcggaaaatc attctctaac aatatgctgg tctattattc agtactcttt ggcggacagg
     2401 caaccattgt tgacccaaaa gc
//
LOCUS       DACEXQ010000281         2421 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.283, whole genome shotgun sequence.
ACCESSION   DACEXQ010000281 DACEXQ010000000
VERSION     DACEXQ010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.283"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(862..1380)
                     /locus_tag="IXH04_002643"
     CDS             complement(862..1380)
                     /locus_tag="IXH04_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAQ1853193.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT          785 a          447 c          353 g          836 t
ORIGIN      
        1 cacctacaac ttataaattg atggtattta gataacacga aagaataaaa ataacagctg
       61 atagtaaagt ttgttgacga ttgctactca atcgaaagtg agttagtgcg tatgaacttg
      121 cttctgaaaa cttatgaaat gaaagacctt ttacatcttg cagaaatcgg tactgggatt
      181 aaactaagca ataaaaacta atgttcccct cattctttga acttctacta tttgatttat
      241 ttattctgat aaatgacgat ttcaccttca ggatcttcaa atcccgtata gaatttattt
      301 aagatctctt cagtatcagt aataggatag gcgataggcc acataccctt tgatggttag
      361 aataaactgc tcaaaatctg tatcgtatgt tggctttagc atattaacaa aggctatctc
      421 caaattataa accataagcg ttgaatcgtt cttaacaatt tcaattcttc ctaacccaaa
      481 gtcagaagat ataattaccg gacttgcctt acccttcatg tgggttgttg agattccata
      541 tttaaaggga attgtcggta ctatcggcgg tctgaaaaac atcccaaaaa taaaaaaatc
      601 tttcccctgc tagtctccta agtccctttg tagcggatat ggcaccataa tctcatttta
      661 tcgaagtgag tcatggttta tctgttggcc aaccaaaatt gatacaatct aacgatgcac
      721 cctctacggc attctagggg agtgcacttg cattcaccca ataaaaaccg gcagttagca
      781 aagaacttat gtagtcgtat gttcttactg ttaacataca agaggtctcc tttccacaat
      841 tgcttagcag taaacgggaa tttacaaagc taatctcttc cattttctta tctttgttct
      901 aaaagtccca aatggagcaa ctgtatttac atgaataaat ttatatactt cccatacagc
      961 cgttttggtt gcctcgtctg cccattttct tttgtgtgca gtgaatagat cttcatcact
     1021 cattgaatca atcatcatgt taatatcatc aatattacga ttgagaattt cctctaattc
     1081 tttcaatggt agatgtgcat aggtatttgt aaaccactta tataactctc ctaactgatt
     1141 ccatttaaat tgatcagaag gcgttttaac agataatcca tttctttcat cattttccca
     1201 ttttagaatt aaattcgtcc aaccaacttg atatgctaaa ttttcagcag gtgttctatc
     1261 cacttcctca actcttttat ccttcaattc ttcaggaata gtttcaaatt ctaaaatata
     1321 tttagtataa gtcctattta tttctttttt taattcttct ttatttttat atgttctcat
     1381 tatattacct ccataaattc ctaattgtct cctttaattc atctataatt tcatccacga
     1441 tattttaatc cgttctacaa tgcatatctg atatgcattg actgtctaac tcatatgtga
     1501 atgatttata aaatatccca acgtctcatc taaagaaatg ttgatcgtca gcaaggagct
     1561 aaaaaatacg aatatatttc ttttgtagta aaatcggact ggtttgcata tcattttctt
     1621 cgtaaatatc aaaatcatgt taactactat caattactaa taaaaactac cactttaagc
     1681 gttaagaact gttaagttta gttaagtgag gatagttcac aaaatccatt gttaaacttc
     1741 acgaaagtgg taaaactcaa aatgctttat ataggattac agcatcgctc tttcttcgat
     1801 tgcgcgttgg gtaaaaaata ttatgaagta aaaattgacg atgacacgtt ccttacggct
     1861 catcagatga aacagctaca aaaacgaaat gatcaactcg aagaaaattt aattctaaaa
     1921 aagcgattgc catattcacg cctcgctcga cgaactatta aaggtcaaga tttacctgaa
     1981 gatcatcctt tttaggaaat tgaagctttt tatgttgcac aatgcattta taatactaca
     2041 atgatgtttt caccagatat tcttctaggg tgcggtgtaa tgaaacagat acacttgcaa
     2101 aagaaaattc accgcgaatt tactaaaatt atgaataatt atgttaatct tccgaaattt
     2161 ttttccaaaa ttaagggatc aagctggtgt tatagattgt ttaaggttgg caaaaaaata
     2221 acttcttttt aaacaattat agaaaaccga tactctgtta agttggttga cacggtgtcg
     2281 atttttatga ctttcttatt ctgaaacttc actaacggac agtacctaaa aagtctatag
     2341 tatggacgtt tacgagtact attttatcaa aaatgctgct catttcaact tatatgtttt
     2401 agtgaactac ccccacttag c
//
LOCUS       DACEXQ010000282         2397 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.284, whole genome shotgun sequence.
ACCESSION   DACEXQ010000282 DACEXQ010000000
VERSION     DACEXQ010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.284"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            206..2068
                     /locus_tag="IXH04_002644"
     CDS             206..2068
                     /locus_tag="IXH04_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1853194.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2068..>2397
                     /locus_tag="IXH04_002645"
     CDS             2068..>2397
                     /locus_tag="IXH04_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1853195.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          864 a          353 c          466 g          714 t
ORIGIN      
        1 aaaatttgaa aaagcgtttg attatttata ttacgactta gtgcaataaa aaacgctttt
       61 taacagtaca tgtagtcgtc tttataatag ataaactctg taatattagt taaaaagcta
      121 taagttgcac ttagttaagt gcaaacattt catattgtcg ttagtttaga aaacgcttat
      181 attagcaatg taaggagaga tgataatgca gcagctaaca aaaagagaaa ggcagctttt
      241 gatttacttg gtggaaatgt ctgaaccggt aacaatcaaa aagttggctg aaaaagaaaa
      301 tgttagtgtt cggacgataa aatacgatct agatgatata agagagtggc tgaatgagcg
      361 gaagcaagtc ctatatagca aacgtagtca gggaatttgg atgaatatta gtagctctga
      421 tcgaatcaaa ttaaaaagcg aattgatgga tgttaatcgt tttgaaatat ttgcagatca
      481 aagtctccga atcaaacgtt tactaattat gcttttagta acaaaagaag ctattactgg
      541 aatgaaaatg gccaacgcat tagatgtcag caaagatacg atcatgaatg atttagatgt
      601 gctggaaaat aattttctta aggaaggatt gaccttgaat cgtcaggcgc gaaagggttt
      661 ttggatcata ggagaagaac gtctagttcg attaacgatt gaagagattc ttcaaaaaga
      721 gttcacggat tatgacattt ataaattaat gtcattgtta ttgaatggcg gtgagactga
      781 atactttgaa atgtattctg ctactgctac acctatccaa gaggtattta atcaagtaat
      841 cattcgaatg cgacacttac tggaatttga aaatctagag aaactgaatt acgcggaatt
      901 actaaatatt ctgattcgag tgacaattgc taccgttcgc ttacgcaaag aggctacgat
      961 tggtcgttat caattggttg ctgaacaaga attagacaaa aacgatcttt cttatcgcgt
     1021 catgcagcag gtctttgatt attatcagtt gccactgttc gaggatgaat atcattatat
     1081 ctatagtgat acattcgaca cgattcacca gcaagacgtc atgaaactag ttgagaatgt
     1141 gatttataaa gtaagtaaag caatcaacca ttctttttat caagatccac aattgttgac
     1201 gaatctgtat gctcatttat ccatgcgatt atcgagaaag aaaaaatttg tcaatgaata
     1261 taatcctttt aaagaagata tcaagcagaa atacccggaa ttatttgatg cgatcaagca
     1321 agcagttcga caagaaataa gtgaacaaaa tattctgatc aatgattctt tcgtggcata
     1381 tattgcctta cactttttgg tttcctacga aagagaagaa gatctacgaa gcgttagagc
     1441 cgtctacatt tgttcaacag ggttgggtgt gactagtctg atcaagcaaa agatcaatga
     1501 agaattaaat aatatcacta tcgtggcatt tgcttcagta ttgaatgcgc aagaaatgat
     1561 tgctaccaaa gatcctgatt tggtgattag tatttttcca attaatggca tcgatcgtcc
     1621 tttcatcaaa gtccatccat tacccactga acaggacttg aaaaaaattc aacaaataat
     1681 aaaaaaaata cttgcttctt ccaatcagca ggaaaggcat aacttgaatc ttagagaaac
     1741 gtccatcaat aaagatactt tagaagactt tagcaaggaa ttgattttga aagcttatac
     1801 gatttatgaa cgtttacttg tcattttgga agcagaattg atccaagaat atcgagaagc
     1861 ttttttgctt catgtattct taatggttca tcgtatcaca tttgatcaac aatatatggc
     1921 agaaggtcgt caagtagacc agattatttt agacaaagga acactggtag gaaaaatcga
     1981 agcagttttt gcagaaaata atttaggaat caatcagtca gaaatcagtg cattattctc
     2041 atatataaaa gaaggagcgt caagttagtg aaaatgtcag acgaggcttt aaaaattatc
     2101 gaggaaagtc aaaatcggga aaagttaaaa caagtgattg atctaattga agagcgatta
     2161 gtagaacatg ctattgtacc aaccgagttg cagtggacca ttctaattaa tcacttgaat
     2221 gagatgctga aacgttctca aagaaaggaa acgattcccg tagtcgatcg ggatttgttc
     2281 aaagaagtat ccaaagaagc cataactatt tctgatgaaa tagttagaag tatcggtaat
     2341 ttaaccgatg atgaaatata tgttttgtcg attcattttg aaacagcaaa aaataat
//
LOCUS       DACEXQ010000283         2373 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.285, whole genome shotgun sequence.
ACCESSION   DACEXQ010000283 DACEXQ010000000
VERSION     DACEXQ010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.285"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            133..>2373
                     /locus_tag="IXH04_002646"
     CDS             133..>2373
                     /locus_tag="IXH04_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ1853196.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVP"
BASE COUNT          815 a          411 c          497 g          650 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta cca
//
LOCUS       DACEXQ010000284         2355 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.286, whole genome shotgun sequence.
ACCESSION   DACEXQ010000284 DACEXQ010000000
VERSION     DACEXQ010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.286"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            143..325
                     /locus_tag="IXH04_002647"
     CDS             143..325
                     /locus_tag="IXH04_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853197.1"
                     /translation="MNKKDIVPVQFSSAAQKSQIDTKPKLACRIKSEDKEIFIYNGMD
                     KYLLYTLLKELNANAH"
     gene            309..494
                     /locus_tag="IXH04_002648"
     CDS             309..494
                     /locus_tag="IXH04_002648"
                     /inference="COORDINATES: protein motif:HMM:NF017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HAQ1853198.1"
                     /translation="MPMLIDATALKHIFIVCGKTDLRKGIDGLAAFVIEEYDLDVYDQ
                     ALFLFCGTKKDRFKALY"
     gene            737..2257
                     /locus_tag="IXH04_002649"
     CDS             737..2257
                     /locus_tag="IXH04_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAQ1853199.1"
                     /translation="MTKMESELLVQIKQLTQLLEIQQQENTLLREQIAEMNRRLFGRK
                     KETPPVDGQIDLLDDSTFNEPEHTGQESQEPITVSSFKRRKRKGLKALSLEGLPEVPI
                     HYELKGADCLCEDCGHTLHDIGATRLREEPLYVPAHIEKQVIYQHAYHCRECEALGET
                     KIKKAPVPKPLINNSLGSSSIVTQSIWEKFVKKVPAYRQEKEWHALGFPLDRKKITNW
                     HIKVSEMVLEPIYNCLHQELSNQEVLHADETSYRVLNTHKEKTFYWLYASGKSEEKQV
                     VLYEHANSRSVDEPKSFLESFSGFLHTDGYAAYGKLPVNNVYCWAHVRRKFFEALGKQ
                     PSPKSHAKIGLDFCDKMFRIERTFNKLSAEERFVARKETLQPVMTEFFHWCESFAYLP
                     KSQLGKAVQYAVNHKDGLQIVLLDGRLELSNNRAERAIKELVIGRKNWLFSKSLKGAR
                     SNGIILSIIQTAVANGLNIRKYLNHLFTEIPNLSSMTPEALRAYLPWNQQIQEICK"
BASE COUNT          808 a          399 c          481 g          667 t
ORIGIN      
        1 ttttcctctt agttctataa aaagtgtggc taatttgagg caaaatatta aattaaatgt
       61 aagcgtgtca taggatagta tctcgtacgc cgattatctt tcttctataa ttaaactatc
      121 aaatatagaa ggagaaaaac gaatgaataa aaaagatatt gtaccagttc aattttcaag
      181 tgcggctcaa aaatcgcaaa ttgacaccaa gccaaaacta gcttgcagaa tcaaatcgga
      241 agataaggaa atatttattt ataatggaat ggataaatat cttttgtata ccttgttaaa
      301 agagttgaat gccaatgctc attgatgcaa ctgcattgaa acatattttt atcgtttgtg
      361 gaaaaacaga tcttcgaaaa ggaattgatg gactagcagc tttcgttata gaagaatatg
      421 accttgatgt ttacgatcaa gcgcttttct tattttgcgg tacgaagaaa gatcgcttca
      481 aggctcttta ctaggatcaa aatggatttg tattaatgta caaaagattt gaaagtgggt
      541 accttcaatg gccgcgttcc agaaaagaag tcttactgac tggcctttca atcgaagaaa
      601 aatgtacgct aaaagaaacg aaaaaaggtg cgatatatta gacgaaaacc ttgttaaatc
      661 aaggattttg tctgtttttc gttgtcataa aatggtataa tagatgtaag aaaatcgcta
      721 gaatggtggt gtaaaaatga caaaaatgga atctgaactt cttgtacaaa tcaagcaatt
      781 aacacagttg cttgaaatcc agcaacagga aaatactctt cttagagaac aaattgctga
      841 aatgaatcga cgcttgtttg gacgaaaaaa agaaacacca cccgtagatg ggcaaatcga
      901 tttattagac gactcaactt ttaatgaacc agagcacact ggacaagaaa gccaggaacc
      961 cattacggta tcttctttta aacgcagaaa acgcaaggga ctcaaagcac tttcattaga
     1021 aggtctgcct gaagttccta ttcattatga attaaaaggt gccgattgtt tgtgtgaaga
     1081 ctgtggtcat actttacatg atatcggtgc gacaaggctt agagaagagc cattgtatgt
     1141 tcctgcccat atagaaaaac aagtcatcta ccaacatgcc tatcattgca gagaatgtga
     1201 agcattagga gaaacaaaaa tcaaaaaagc gcctgttcct aaaccgttga tcaataatag
     1261 tctaggttcc tcttcaattg tgacacagag tatttgggaa aaatttgtta aaaaggtgcc
     1321 ggcatatcga caagaaaaag aatggcatgc acttggtttc cctcttgatc gtaaaaaaat
     1381 cactaattgg catatcaagg taagtgaaat ggttttagag ccaatctaca attgccttca
     1441 tcaagaatta agcaatcagg aggtcttaca cgcggatgag acaagctatc gggtattgaa
     1501 tactcataaa gaaaagacct tttactggtt atatgcttca ggtaaaagcg aagaaaaaca
     1561 agttgtgctc tacgagcacg caaattcgcg ttccgttgat gagccaaaaa gttttttaga
     1621 atcatttagc ggtttcttgc atactgatgg ttacgcggcc tatggaaagc tgccggtaaa
     1681 caatgtttat tgttgggcgc atgttcgcag aaagtttttt gaagcattgg gtaaacaacc
     1741 gtccccaaaa agtcatgcca aaataggatt agacttctgt gacaagatgt ttcgaatcga
     1801 acggaccttt aataagctta gcgcagaaga aaggtttgta gcgaggaagg aaacgttgca
     1861 gcctgtgatg actgaatttt ttcattggtg tgagagtttt gcttatttgc caaaatcgca
     1921 gcttggaaag gcggttcaat acgcagtcaa tcataaagac ggacttcaga ttgtgttgct
     1981 tgatggacgc ttggaacttt caaataaccg agcagagcgt gccatcaaag agttagtgat
     2041 cggtcgaaaa aactggctat tttctaaaag cttaaaaggc gctagatcaa atggaattat
     2101 attgagtatt atccagacag ctgttgcgaa tgggttaaac atccgcaaat atttaaatca
     2161 tctttttaca gagattccca atctgtcttc gatgacacct gaagcgctac gcgcttattt
     2221 gccatggaat cagcagatac aagaaatctg taaataatat aagccacaaa taacctgatt
     2281 tgagtgtatc aaatcgggct atttgtgttt aggtaccatt ctatgacacg cttacgctca
     2341 aaactactga attat
//
LOCUS       DACEXQ010000285         2354 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.287, whole genome shotgun sequence.
ACCESSION   DACEXQ010000285 DACEXQ010000000
VERSION     DACEXQ010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.287"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(193..468)
                     /locus_tag="IXH04_002650"
     CDS             complement(193..468)
                     /locus_tag="IXH04_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAQ1853200.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            576..1340
                     /locus_tag="IXH04_002651"
     CDS             576..1340
                     /locus_tag="IXH04_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAQ1853201.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            1463..1966
                     /locus_tag="IXH04_002652"
     CDS             1463..1966
                     /locus_tag="IXH04_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1853202.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSPSMRKRWQRY
                     FGKESSI"
BASE COUNT          736 a          426 c          515 g          677 t
ORIGIN      
        1 tcactgcaag tgatagatca gctttttcac tgctgtcctt aatttttgag gcaaagcgcc
       61 tcaagaataa ccttatcgac ggtgttcaaa agcttgcatc tacggcatta agtccagcct
      121 gttgaaaaat atggagagct atttttcaca acctgtcctt aatgcttgat gcagaccagc
      181 tttttcacaa ccttatccca ccacatcaaa tttctttcgt tcctcaatca tcgggtcttc
      241 ttggacatcc acatactgca ctcgtccact tgggctcgga gatttcttga cttcttcaat
      301 aaatttctgg atgatgtcgt cattgccgat tgcttcgatc gtgactgagc cgtcatcttc
      361 gttttttacc gttccagtca cgcctagttg atcagcgacc atcttggtca tgtagcgaaa
      421 tcctacgcct tggacgcgtc cttggacgtt cattttaact ttgcgcatgt tcgtcacctt
      481 tctttcctat tttataaatt aatgataccg atttttttag gatgaaacaa gctcaagcac
      541 ctattccatg atataatcat ggggaggtga ggaatgtgaa agagatccaa tcagcaaaaa
      601 atccaagaat caaagaatta aaaaaactac ataaaaagaa atatcgggaa gaaaaggaag
      661 aatatctttt ggaagggttc catctgattg aagaagctgc gtcgtctgat gcagaaataa
      721 aagaactttt tgtgacacag cgaggcatga aagaatgggg agactggctg gccgcaaaca
      781 aagagatttc tacttttcta gtttcggatg aggtcatgga tgctgtatcg gatctaccta
      841 caccacaagg aatcttagca gtgatggcgt taacggctga aggcacgcca gatttttcag
      901 gaggatggct gttgttagat aatgtacaag atcctggaaa tgtagggacg atgatccgta
      961 cggcagatgc atttgatatg gctggtgttt tattaggcaa aggaacggca gatatttata
     1021 gtacaaaggt gctgcgcagc atgcagggaa gcaattatca tttgcctgtg ttgcaaaaag
     1081 atttgacaga agtgattcct ttaatgaaag acaaagggtt tacagtgtat ggcacagaat
     1141 taaatgaaga agcacacgca ttagacaaag tagaaaaatc agataacttc gcaataatca
     1201 tggggaacga aggtcagggg gtaagtcagg aaatcttgtc tttgactgat cagaacgtat
     1261 atatcccgat gaaaggaaat gcggaatcgt tgaatgttgg ggttgcggca gggattgttc
     1321 tttatcattt ttctttatga ttaaggaact attaagcttt ggctatttta tccaaggcga
     1381 ctagaataat ccgaacactg gtggtatact tagatcaatt tcagaagaaa atgaaattca
     1441 aaaaggtggc agatcgataa ttatgacaga aaaatggcgt gaagacgaag aatatatgtc
     1501 ttatgtagaa gatcttttag aaacagaagc agtgaaaaaa ttagcgaatt acacacagca
     1561 tgttcattcc actcgtttgg agcattccat cagtgtttct tactatagtt atctgctcgc
     1621 aaaaaaatgg ggtggcaatg ctaaagcaac agcgcgtgct ggacttttgc atgatctgtt
     1681 ctactatgat tggcgtacga cgaaatttga tgaaggcaca catgcgtata tccatccaag
     1741 aattgctgta aagaatgcag aaaagatcac cgaattgtcg gatcttgagc gagatatcat
     1801 tttgaagcac atgtggggcg caacgattgc accgcctaag tacaaagaag gatatatcgt
     1861 aacattcgta gacaaatact gcgcagtcaa agaagccgct cagcctatgt caccaagtat
     1921 gcgtaagaga tggcaacgtt actttgggaa agaatcctcg atttaataga taaaaccaat
     1981 aatagaccat attgaggttg tgacagaagt ggtcagcttc aagaaataag aaggaattta
     2041 cgaaaattgc ttctcaaatt tttgtgaagt ttcggcttat taccgcagat gccagctttt
     2101 gaacaccatt cattcgaaac aagggacttt gacacgactt ttgtcacagt ccttttttct
     2161 gtttgcagtt gactatctgt tcaagatacg gtttactttt atttatcgaa tgaatcatgt
     2221 acatagaata ataagcatta aagggggtga tgataagatg caaaacgtat cttatttaaa
     2281 agagtccggg atcttcaggt aacacatact ttgcagaaga tcactttttt ctacaaagta
     2341 tgcactttgg ttag
//
LOCUS       DACEXQ010000286         2338 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.289, whole genome shotgun sequence.
ACCESSION   DACEXQ010000286 DACEXQ010000000
VERSION     DACEXQ010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.289"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            12..250
                     /locus_tag="IXH04_002653"
                     /pseudo
     CDS             12..250
                     /locus_tag="IXH04_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209247.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            252..1550
                     /locus_tag="IXH04_002654"
     CDS             252..1550
                     /locus_tag="IXH04_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAQ1853203.1"
                     /translation="MKHTHKIILAVTSVCTVIILGVSIFFVTQAATNNKLENTSQTTQ
                     HSSSSSKPVEDKQTTKQLDQAKQLAASYHYDEAIALLEKDDAKEAQQLLATLKKEKES
                     LVKWEDPTKISHVFFHSLIVDPAKAFHTQQAQGYKDYMVTISEFNKTIDQLYKNNYVL
                     VNLNGLVKKGTDGKLTFTGVSLPEGKKPLILSQDDVSYYEYMDNSGFPSKLIVDKQNQ
                     IKNIYIDNKKETVGDYDMVPLIDSFIKKHPDFSYQGAKGTLALTGYNGVLGYRTSKSE
                     YGDNEKTHKEIEAAKKVADQLKEDGWSFASHTWGHLNMTQASLADIQQDNERWQNEVA
                     PILGKTNILIYPFGADISDWQPYSEANQKFAYLKQQGFDIFCNVDASTPAWGQLGTDY
                     YRNARINIDGIRFEADLKGENPILDHFINVKEMYDQKDRG"
     gene            2197..2283
                     /locus_tag="IXH04_002655"
                     /pseudo
     CDS             2197..2283
                     /locus_tag="IXH04_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
BASE COUNT          835 a          399 c          425 g          679 t
ORIGIN      
        1 tttagttttt cttggaaata aaaacagcga aaatccgaac attttcacct ctctcacttt
       61 ataactttta caaaatagta cttctcataa aaagcgctct agttaaagaa aataagagaa
      121 aaatcaaaaa aaacaaaagt ttcttttaca cttgtttcta tattcgttac aatggttatg
      181 ggaagttttt aaaaaggtta agaaaagctt acgggggttt ttgaaagcaa aaagtagtag
      241 gggacgataa aatgaaacat acacacaaaa tcatattggc tgtcacttct gtatgcactg
      301 ttattattct aggtgtaagt atcttttttg ttacacaagc agcaacaaac aataaattag
      361 aaaacactag tcaaacaact caacactcaa gcagcagctc aaaacctgtt gaagataaac
      421 aaacgacgaa acagcttgat caagcaaaac agcttgctgc aagttatcat tatgatgaag
      481 ccatcgcctt acttgaaaaa gacgacgcca aagaagcgca acaattgtta gcaactttga
      541 aaaaagaaaa agaatccttg gtaaaatggg aagacccgac caaaatctca cacgtcttct
      601 ttcatagtct gatcgttgat ccagctaagg cttttcatac ccagcaagca caagggtata
      661 aagattatat ggtcactatt tccgaattca ataaaaccat tgatcagtta tataaaaata
      721 actatgttct tgttaatcta aatggattag tcaaaaaagg aacagatgga aaactgacct
      781 ttacaggtgt ttctcttcca gaaggaaaga aaccgttaat tctttcgcaa gacgatgtca
      841 gctattatga atacatggac aattcaggat ttccaagtaa gttgattgtc gataaacaga
      901 accaaatcaa gaatatttat atcgacaata agaaggaaac tgttggtgac tatgatatgg
      961 ttccattgat cgattcattc attaaaaaac atcccgattt ttcttatcaa ggcgccaaag
     1021 gaacattagc tttaacaggc tataatggtg tattaggtta ccgtacatca aaatcagaat
     1081 atggtgataa cgaaaaaaca cacaaagaaa tcgaagcagc caaaaaagtt gctgatcaat
     1141 taaaggaaga tggttggagt tttgcttctc atacttgggg acacttgaat atgacacaag
     1201 cttctttagc cgatatccaa caagataatg aacgttggca aaatgaagtt gcacctattt
     1261 taggcaaaac aaatattttg atctacccat ttggcgctga catcagcgat tggcaacctt
     1321 attctgaagc aaatcaaaaa tttgcttact tgaaacaaca aggatttgat atcttctgca
     1381 atgtcgatgc ctcaactcct gcatggggac aactaggaac tgactactac cgtaacgcca
     1441 gaatcaatat tgacggtatc cgtttcgaag ctgacctaaa aggagaaaat ccaatacttg
     1501 atcactttat caacgtcaaa gaaatgtatg accaaaaaga tcgaggatag aggttgtgaa
     1561 agaagcgtcc agctccgagc aaataagaac tagatttcta aaatagtttc tcatattttg
     1621 gaaatctagc gcttattgct gaggagttgc ttcttgaaca ccgtgaatag aggttgtaaa
     1681 aagagcgttt tgacttattg ttgaaggtca gctctttgaa caccgcgtgt taggttgtga
     1741 aaaggctggt ctgcatcaag caataagaat gaacagagaa aaacagcttc ttgaataccg
     1801 cagataggtt gtaaaaaaac cgttttgacc tgacgataac cagtaaaaca gaggtgagac
     1861 cattgctaaa agtagcagtg gtctcatctc tttttttgca caagtcatat ttgattgttt
     1921 cgttatttta atcaggataa agttcttttt ttcatgctaa agagtgattc tttcttctcg
     1981 attttcactt tcttcgttta acttttggta aatacttctg tcagaaaaag ctctaggtag
     2041 acacacgaaa gcagataaaa agcctaactt ttgcaacagt ttatgctgtc gttcaaagtt
     2101 aggcttttac agtttataaa tagggttctg ttgcaaagtt ttccaaaaaa tctattttag
     2161 tgtaaaattg agaaaaaaga cagagaggac agagtaatga atcattttaa aggcaaacaa
     2221 ttcaaaaaag acgtcattat tgtcgctgtt ggttactacc tgcgttacaa tctaagctat
     2281 cgttaagttc aggaattgtt atatgatcgt ggaataaatg tttgtcatac tacgattt
//
LOCUS       DACEXQ010000287         2310 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.290, whole genome shotgun sequence.
ACCESSION   DACEXQ010000287 DACEXQ010000000
VERSION     DACEXQ010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.290"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            5..1087
                     /locus_tag="IXH04_002656"
     CDS             5..1087
                     /locus_tag="IXH04_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1853204.1"
                     /translation="MNDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTDT
                     RVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINAE
                     FKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKIIS
                     LEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVKN
                     DGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKEDG
                     ELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISIT
                     MDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1181..>2310)
                     /gene="lepA"
                     /locus_tag="IXH04_002657"
     CDS             complement(1181..>2310)
                     /gene="lepA"
                     /locus_tag="IXH04_002657"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAQ1853205.1"
                     /translation="NGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQDTRVG
                     DTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQFEPE
                     TSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTATVDNP
                     ADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVNVVYN
                     IPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFIVHRD
                     FAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGGDVSR
                     KRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
BASE COUNT          726 a          462 c          443 g          679 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaaccac cggtggcaaa ccggtggcaa atccatgtaa
     1141 acatggttta ttttttacac tgttttcaaa caaacccttg ctatttcttc ggttcgtcct
     1201 cgtccatttt cagaacggcc atgaaggctt cttgcggaac ttcgacggag ccgatttgtt
     1261 tcatccgctt cttcccttct ttttgtttct ctaagagttt acgtttacga gaaacatctc
     1321 caccataaca tttagccaag acgtttttac gcaaggcttt gatgtctgaa cgggcaacga
     1381 ttttttgtcc aatagctgct tggattggca cctcaaattg ttggcgtggg attagttttt
     1441 tcagtttttc aacgattgct tttccacgtt cataggcaaa gtctcggtga acgataaagc
     1501 ttaatgcatc gactttttct ccattcagca agatgtccat cttcactaac ttgctttttt
     1561 gataaccaga catttcataa tccaatgagg catagccttt cgtgcttgat ttcaatttgt
     1621 cgaaaaagtc aaagacaatt tcagaaagcg gaatattata gacgacattt actcggtaat
     1681 catctaaata atccatcgta atgaattctc cccgttttcg ttgagaaagt tccattactg
     1741 caccgacaaa atcatttggg accatgatct gtgctttgac gaatggttct tctacatcct
     1801 gaatcgttac aggttcagga aaatctgccg ggttatctac tgtagcagtc gtaccatccg
     1861 ttttattaac atggtaaata acggatggtg cagtcgtgat caattctaga ttgaattccc
     1921 gttccaagcg ttcttggacg acatccatat gaagaagtcc gaggaatcca cagcggaacc
     1981 caaaacctaa tgcttgagag gtttctggtt cgaattgtaa agcagcatca ttcaattgca
     2041 gtttttctaa agcttcccgc aaatcgttgt aacgagaagt atcaattgga taaagaccac
     2101 aataaaccat cggattcatt tttcggtaac ctggtaaagc ttcagcggca ggattgtctg
     2161 ctaacgttac tgtatcccct actcgtgtat cttggacagt tttgatactt gctgtgatat
     2221 aaccgacatc accgaccatc aaaaagtctc tagcaactgc ttttggcgag aagaccccga
     2281 cctctgtaac atcaaatgtt ttgccattac
//
LOCUS       DACEXQ010000288         2307 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.291, whole genome shotgun sequence.
ACCESSION   DACEXQ010000288 DACEXQ010000000
VERSION     DACEXQ010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.291"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..548
                     /locus_tag="IXH04_002658"
     CDS             <1..548
                     /locus_tag="IXH04_002658"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="catalyzes the formation of 1-deoxy-D-xylulose
                     5-phosphate from pyruvate and D-glyceraldehyde
                     3-phosphate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAQ1853206.1"
                     /translation="PNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            632..1090
                     /locus_tag="IXH04_002659"
     CDS             632..1090
                     /locus_tag="IXH04_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAQ1853207.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            1199..1882
                     /locus_tag="IXH04_002660"
     CDS             1199..1882
                     /locus_tag="IXH04_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAQ1853208.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            2029..>2307
                     /locus_tag="IXH04_002661"
     CDS             2029..>2307
                     /locus_tag="IXH04_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAQ1853209.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEE"
BASE COUNT          828 a          367 c          488 g          624 t
ORIGIN      
        1 tcccgaacat caaatatctt gctccagcaa caaaagagga tatgatctct atgctagatt
       61 gggcgttcga acaaacagcg cagcctgtag caatccgaat tcctacacat ggtgtagaac
      121 atggtacagc tatcgcaaat gattatagta ttccagccta tcaaacagta aataatggag
      181 gaaaagtagc aattttagct ttaggcggtt ttcttgaatt aggaaaagaa acagtaaaag
      241 cttttagaaa aaaaggaatc gaagcaacgt tgatcaatcc aggaacgatc acagaaaatg
      301 acgtagaaac attagataaa ttaaaagaaa cacacgaatt agtcatcaca ttagaagatg
      361 gtagtttgtc aggaggattt ggcgaaaata tcagtcgtta ttatggtgat tcttcaatga
      421 acgtattaaa ttttggtgca gagaaagaat tcacggatag cgttcctgta gggcagcttt
      481 atgaacgtta tcatttaaca ccaaaacaaa tcgttgaaga tagtctgcgt gtcttgaata
      541 ttcaataatt cattatgctc accatccaga taattgttta tactagtatc tggatggttt
      601 tttcattata aaaagcaaag gggtggatgt tatgtatcag caagtagaag aatatgtcaa
      661 aaagttccaa gaaaaatatc ctagttatca atcagaaaaa ttaggtttct tttcatttgg
      721 ggatacagaa aaaatggcag acgagttgag cgaactagtt gtaaaaaaga taaagactgg
      781 tacatcaagt ggttacgatc tgtataaaca agagaatgag cctttaccaa aagttggtca
      841 attagacgtg gtcctgaatg gaaaaaatga accagtttgt atcatcaaaa atataaatgt
      901 gaccatcttg ccttttagtg aagtcacgaa agagcatgct tggaaagaag gagaagggga
      961 tcggacatta gcttattggc ggaaagtcca tgctgagttt tttaaacatg cttataaaga
     1021 agcgttgaac atagaattta cagaagagaa gctggtcgta tacgaagaat ttgaagtaat
     1081 tttcttttag taagaaaggt ctgctgaaag aaatagctca ttattgtctc ctgaatagaa
     1141 aagggttaca tttttatatg aagctgtatt tttatataac attactggag gagatattgt
     1201 ggatacactg gaacaaatca aaggcggagt cgtcgtttct tgtcaagctt tggaaaacga
     1261 gccgctacac agttctttta ttatggggag aatggctttg gctgcaaaag aaggcggagc
     1321 agttggtatt cgagcaaata cgacagaaga tatcaatgaa atcaagcgaa tcgttgattt
     1381 accggtaata gggattatca aaagagatta cgatgggtca gaagtgttta ttactgcgac
     1441 agaaaaagaa gtggaagaac ttttggagac gcaagcagaa atcattgcac tggatgcaac
     1501 ttctcgtaaa cgtccgacag aaaaaacaac tgctgaactt gtccaaatga ttcacgaaca
     1561 cggtcggtta gctatggcag atatttcgac ttttgaggaa gcgcttcaag cgcaagaaga
     1621 tggttttgat cttttgtcta cgactttagc aggctataca gaatattcac gaaagacgca
     1681 gacacctgat tttgatttat tgaatcaaat catagaacaa gtcgatatcc cagtaattat
     1741 ggaaggacat actgactctc ctgaacaagt agagaaagca tatgaattag gtgctttttc
     1801 agtcgtagtt ggatctatta tcacccgtcc gcaattgatc actaaaagat atgtcgaagc
     1861 cgcaagtaaa gcaaataact aaaaaacacg cacggcacag attcttgaca aaaaagaaaa
     1921 attgatagat agtttaaagc agattctaag aagatgaaaa aagcattact tttcattttg
     1981 tcttcaagat ctgcttttta acgaatttta tagaaagaag gcgaaaccat gtttgaacgg
     2041 agtccttcag aattgacaga acgtgaaaat tacaagttgc tgattggttc agtcatccct
     2101 agaccagtag cagtagtaac gactaaatca gcaaaagata tagtgaatat cgcacccttc
     2161 agttacttca atatcgtcag ttcgaatcga cccatcttat ccttggcgat ccagagaaaa
     2221 caagatgcag taaaagacac agcaaagaat attcttgaga caagagaagc tgttatccat
     2281 atccttgatg aggacaacgt agaagaa
//
LOCUS       DACEXQ010000289         2296 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.292, whole genome shotgun sequence.
ACCESSION   DACEXQ010000289 DACEXQ010000000
VERSION     DACEXQ010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.292"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            69..638
                     /locus_tag="IXH04_002662"
     CDS             69..638
                     /locus_tag="IXH04_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1853210.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(816..1256)
                     /locus_tag="IXH04_002663"
     CDS             complement(816..1256)
                     /locus_tag="IXH04_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAQ1853211.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLSGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            1414..2178
                     /gene="map"
                     /locus_tag="IXH04_002664"
     CDS             1414..2178
                     /gene="map"
                     /locus_tag="IXH04_002664"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAQ1853212.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
BASE COUNT          768 a          387 c          473 g          668 t
ORIGIN      
        1 aaaacatgtt acaatgaaat taatcaatgg tggaagcgtt ataaaaaaat taaggagatt
       61 aaaaaaatat ggcaagaaaa aagacgatta ctagagaaca gattttggca gctgcttatg
      121 aagtagctgc caccgatgga tttacacgat ttactgcgag aaacatcgcg aacaaaatga
      181 attgttccac gcaaccgatt tatcttgagt ttaaaaatat ggatgacttg agacaagctc
      241 tttttgagca gatctatgat catttacaat atgaagtgtt cactgttgag catacaggga
      301 atacgattat cgatcttgct tttaattata tccattttgc aagaagagag aagaagttgt
      361 atcgttcact gtacttagaa gaatatggtg gaggaaaaga gatgcagaat ttctcttata
      421 cttactttgt gaatacagtg aaacgcgatc cagcttatgt gaatctttca gaagagcaga
      481 tcgaatcttt gtataatggg acatggatca tagctactgg gatcgcagct ttgatgtctt
      541 caagcatcat ccatccaact gatgaacaga ttgaaaagat gatacaagac agtatagatg
      601 ctatcttgaa attcaaagaa ccaatagaaa tcgattgaat atactcaaaa gttccacaaa
      661 tcagtagata acaagagatg tgtcaaaata aggaaaagtt cgtttttctt acagcgtatg
      721 catttttaac tcccgaagaa ctcttccatg aatgcaatga ataaaagagc cgtacagatt
      781 gaataaatct ggatggctct tttattgcaa gtatattaaa tttttgcgac tagttcttta
      841 gcgaaagctt ctaaattttc gatatcttct tcttcagcat tcagatccac tttaacattt
      901 tcggctcctt ttgatgcgcc tgtttttgca aaagcagcat cgaaatcatc caccgatttg
      961 cagaattcat cgtaaaaagt atcaccagat ccgcagacac cgtaaatttt tcccgataga
     1021 tccaattctt gcaagtcttc ataaaagtct acgatctcat caggcagatc gccatcatcg
     1081 tatgtataag tagctactac gcaaatatct gcctcctcga attcatctgc atctacttgg
     1141 gtacattcgt tgatttcgac ttcgatatcc agattttcaa atgcttctgc aacaatatcg
     1201 gcgatttctt cagtgtttcc tgtcatgcta gcatatacga tttttgctaa agccatgatg
     1261 tgcctcctcg tttataatta tttaacaatt atgaattata ccaaaacatc attgaaaaga
     1321 aaataaaacc tagagacacc tggggaatgt catttggcta aatgtatgcc gctatgttaa
     1381 aatgagaggg atttgtaaaa aggagacgaa aatatgatta ctttaaaatc agcacgtgaa
     1441 attgaaatga tggcagaatc tggagcttta ttagcagatg tacataaaaa tctgcgtgat
     1501 tttataaaac caggaatcac aagttgggat attgaggtat ttgtcagaaa ctatatcgag
     1561 agtcatggcg gaattgctgc gcaaattggc tttgaagggt atgaatatgc gacttgtatc
     1621 agtataaatg atgaaatttg tcatggattc cctcgtaaaa agcctttgaa aaacggtgat
     1681 ttggtaaaag tcgatatgtg tatcgatttg aaaggcggca tttctgattc ttgttgggcc
     1741 tatgtagttg gagaatctac acctgagatc gaccgattga tggaagtaac gaagaaagct
     1801 ttgtacatcg gtattgagca agcacaagta gggaatcgga ttggtgacat cggtcatgct
     1861 atccaaactt atgtagaagg cgaaaatcta gcgatcgttc gtgattttat tggtcatggc
     1921 gttggtccta cgattcatga agaaccagca gttcctcatt acggagaagc tggaaaaggg
     1981 ttgcggttga aagaaggcat ggttatcacg atcgaaccta tggttaatac cggaacttgg
     2041 aaaatgaaaa tggatcctaa cggctggact gcgtatacaa gagacggcgg actttcttgc
     2101 caatatgaac atacattggc tattactaaa gatggtccaa gaattttgac atcacaagga
     2161 gaagaaggca cttattgatc tatccagtca ataggtacgg gagaggtaca tgaaagtttg
     2221 gaacaaaata aaacaaaatg aaaatctgat gcgctttatt gagacgactc aaaagcgcat
     2281 cactgattca gaaatg
//
LOCUS       DACEXQ010000290         2279 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.293, whole genome shotgun sequence.
ACCESSION   DACEXQ010000290 DACEXQ010000000
VERSION     DACEXQ010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.293"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..1069)
                     /locus_tag="IXH04_002665"
     CDS             complement(<1..1069)
                     /locus_tag="IXH04_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAQ1853213.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQAS"
     gene            complement(1171..1422)
                     /locus_tag="IXH04_002666"
     CDS             complement(1171..1422)
                     /locus_tag="IXH04_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAQ1853214.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            1569..2192
                     /gene="lexA"
                     /locus_tag="IXH04_002667"
     CDS             1569..2192
                     /gene="lexA"
                     /locus_tag="IXH04_002667"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAQ1853215.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
BASE COUNT          607 a          495 c          420 g          757 t
ORIGIN      
        1 ggcttgcttg actgcttcca acttcataag ttggcaattc tgcatcccag ttttctggta
       61 gtttgccatc aaaagcatct tcaaattgtt gtgctaattc aggataagct tttttataat
      121 tggcaaacat ttcattccat gcatcttcag ctttttgtcc ttcttctatc atcgtttgat
      181 ggaagcgggc agctacttct tctggaacag tgaaatctgg atactcccaa ccataaactt
      241 ctttagccat cttgatgcca tcttcaccaa gaggagcacc atgaacagca gatgttcctt
      301 ctttaggagc accatagccg atgactgttt ttacttcgat caatgttggt ttgtctgttt
      361 cagcttttgc ttcttcaatc gctttagaga ttgcttctag gtcatttccg tctttcacta
      421 agatatgttg ccagccatat gcttcatagc gagcaccaac gttttctgtg aaagcttttg
      481 aagttggacc gtctagtgaa atatcgtttg aatcgtataa aacgatcaat ttccctagtt
      541 tcatatgacc tgccatcgaa cttgcttctt gagatacccc ttccatcaag tctccgtcac
      601 cgcatattgc atacgtatag tgatccatca cattaaagtt ttctttgtta tacgttgctg
      661 caaggtgcgc ttctgccatt gccataccga cagccattgc aatcccttgt cctaatggcc
      721 ctgtagtggc ttctacacca tctgtatgat gtacttcagg atgtcctggt gttttagaat
      781 cccattgacg gaattgtttc aaatcatcga ttgtcacttg atagccagct aaatgaagca
      841 agctatacaa cattgctgac ccatgtcctg ccgaaagaac gaaacggtcg cggtcagccc
      901 agttttttga tgtcgttgga tttactttca ggtgttttgt ccataacgca tatgccatag
      961 gtgctgcgcc catcggcaat ccaggatgtc ctgagtttgc tttttggact gcttcaatac
     1021 ttaatgtacg aattgtgtta acgccaagtt ggtcaatttt gtcaaacaaa ataacccctc
     1081 ctattaattt aggttttttt tacctttatt tcttacactt tcattctata agatgtcaca
     1141 cgaaaaagca atctttttca gtatttttta ctaaaagttg tcatttcggt tgtgtaagcc
     1201 ttttttcttt tggatttctt ttaatttttc cggggtcacg tcattgccct caggatcgac
     1261 gactttcatt ccttcgatat gatgacgcat accggaacga aaagcttcca aatactcttt
     1321 gcgcaagttg atttgttctt gttcttcggc ttttgagaga ccattttcct tctttttacg
     1381 tgcaagttca ttgatccgct ctattttttc aggtgacagc atgctcagcc ctccttttac
     1441 tctccttcat attatataaa aactgaaaaa aaaacaacta gataaactaa ataaaatacg
     1501 aacattcgtt tgatttagcg ctttttatat ggtactattt atttatcaag tgaaagaagg
     1561 tgtaccttgt ggcccgacaa actgaatcaa gacaaatcga agtcttacga tatatccatg
     1621 aacaagtaac ccaaaaagga tacccgccga ctgtccgtga aatcggggaa gctgttcagc
     1681 tttcttctac ttctactgtt cacggtcatc ttgcacggct tgaaaaaaaa gggtttatcc
     1741 aaagagatcc gacgaaacct cgtgcaatcg agttgacaaa acaaggttta gaaaagattg
     1801 ggattcgtcc aacaactatt ccagtacttg gcgtcgtaac agctggagaa cccattttag
     1861 cagtagagga tgcttcagac ttctttccaa tccctcctaa tctttcttcc gaagaaggca
     1921 gtctctttat gctgacaatt cgtggagata gtatgatcaa tgctgggata ttggatggtg
     1981 acaacgtgat cgttcggaag caagaaactg ctcaaaatgg cgatattgtc attgcaatga
     2041 ctgccgagga cgaagcgact tgcaaacgat tctataaaga aaaaaacttt ttccgtctgc
     2101 agcctgaaaa cgacttgctc gaaccaatca tcttagatca agtatctata ttaggacgag
     2161 tagtaggact ttatcgtagt catatctatt aattgctact aattaaaaga aaacttttta
     2221 aaaagagttt gagacgttgg tctcaaactc ttttttcgcc cgggttcttc ttctttcaa
//
LOCUS       DACEXQ010000291         2255 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.294, whole genome shotgun sequence.
ACCESSION   DACEXQ010000291 DACEXQ010000000
VERSION     DACEXQ010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.294"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..507
                     /locus_tag="IXH04_002668"
     CDS             <1..507
                     /locus_tag="IXH04_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HAQ1853216.1"
                     /translation="KLLKIMKSHPDYNAWRYTRRMRITSYYYSKRKKNIIYAIMYIVS
                     CRRMNSLGRKCGIECGENVFDEGVRFYHTQGIVINGNAIIGKNCYLYGNNCIGNDGID
                     PKCPVIGNNVRVCVGAKIIGNVKIANNVVVAAGAIVIKDCIENGAILAGVPAKIIGYV
                     NLTKVFLE"
     gene            534..1844
                     /locus_tag="IXH04_002669"
     CDS             534..1844
                     /locus_tag="IXH04_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAQ1853217.1"
                     /translation="MRISIKRWSGADWTLFTLAFFAISIVLVYTFNAPRAILYLGDII
                     NIFVLLNALSFRRRKGIYASKNEMLIVLFLLVSICSSVINLENIGLIIWGIRNNTRMF
                     IVYINCATYLTIKDYYKVFAIIEKLFWISLPLCLIERFMVTYPTGTIVGDMIGGVFWN
                     YSGCNMPLNLILCVTVTVVTIGYFKGEKNFIHFILCVCAALFMSALAELKVFIPELFI
                     IFVAIVFTLKVDFKVVIKILSCVILVSIVLSFFITIFITLNDNGQSYADNFTIQGLLE
                     YASRDSGYDGVGDLNRITGISRVVDEIFNNNFRLVLMGVGIGNAEFTNFFVSEFYKSF
                     GNLHYQYFHHIWLFIEVGILGVSIFLFFIIKTFFNAKRSFMKDKDASLVKVIAIMSVF
                     FFVYNTTLRGETTGPLIYMILAMPEVVKRKEHYYSEKNTKGEIL"
     gene            1841..>2255
                     /locus_tag="IXH04_002670"
     CDS             1841..>2255
                     /locus_tag="IXH04_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAQ1853218.1"
                     /translation="MIPKKIHYCWFGGNPLPKKDRKCIESWKKYCPDYEIIEWNESNY
                     DLNKNRYMADAYKERKWGFVPDYARFDIIYNHGGFYLDTDVELIKSLDILRENHAYMG
                     FEGEIWVNGGIGFGAEEGHSVIKGLRDIYDSLSFYQ"
BASE COUNT          762 a          240 c          451 g          802 t
ORIGIN      
        1 aaattactaa aaataatgaa atcacatccg gactacaacg cttggcggta tactcgtcga
       61 atgagaatta catcttatta ttattcaaag agaaagaaaa atatcattta tgcgattatg
      121 tatattgtgt cttgcagaag aatgaatagt ttgggaagaa aatgtggtat agagtgtggt
      181 gaaaatgtat ttgatgaagg tgtaagattt taccatacac aaggaattgt tattaatggt
      241 aacgccataa taggaaaaaa ttgttactta tatgggaata actgtattgg aaatgatgga
      301 atcgatccta agtgtcctgt aattggaaat aatgtacgtg tatgcgtggg tgcaaagatt
      361 attggtaatg taaaaatagc taacaatgta gttgttgcgg caggagctat tgttatcaaa
      421 gattgtatag agaatggtgc aattcttgca ggtgtaccgg caaaaataat agggtatgtt
      481 aatttaacaa aagtattttt agaataattt ttagcgaaag aaggtgaata acgatgcgta
      541 tttcaataaa aagatggtca ggagcagatt ggacattgtt cacgttggca ttttttgcaa
      601 tctccatagt tcttgtatat acttttaacg ccccaagagc aattttatat ttgggagata
      661 taattaatat ctttgtgtta ttaaatgcac ttagctttag acgcagaaaa ggaatatatg
      721 ctagcaaaaa tgagatgctg atagttttgt ttttactagt ttctatttgt agctcagtta
      781 ttaatttaga aaatatagga ttgattatct gggggatacg aaataacact cgtatgttca
      841 tagtatatat aaattgtgca acatatttaa caattaaaga ttactataaa gtattcgcta
      901 taattgaaaa attattctgg atttcactgc cactttgttt aattgaaaga ttcatggtca
      961 cctatccaac tggaacaatt gtaggagata tgataggtgg agttttttgg aattattcag
     1021 gttgtaatat gccattgaat ttaattttgt gtgtgacagt tactgttgtt acaatcggat
     1081 atttcaaggg agaaaaaaat ttcatccatt ttattttatg tgtttgtgca gcacttttta
     1141 tgtctgcatt agctgaattg aaagttttta ttcctgaatt atttataata tttgttgcaa
     1201 tagtattcac tttaaaagtt gattttaaag tagtgatcaa gatattatca tgcgtaatac
     1261 ttgtgtcgat agtattatcc ttttttataa caatatttat tacattgaac gataatggac
     1321 aaagctatgc tgataatttc acaattcaag gtcttttaga atatgcgtca agagattctg
     1381 gatatgatgg tgttggtgat ttaaatcgaa taactggtat ctctagagta gtagatgaaa
     1441 tatttaataa taattttaga ttagtcctaa tgggagtagg cattggcaat gcagagttta
     1501 ccaatttttt tgtcagtgag ttttataaat cgtttggtaa tcttcattat cagtattttc
     1561 atcatatttg gttatttata gaagttggaa tattaggagt ttcgatattc ctatttttta
     1621 taataaagac atttttcaat gcaaaaagat cttttatgaa ggataaagat gcaagtcttg
     1681 ttaaagttat tgcaattatg tcggtattct tttttgtgta taacacaaca cttaggggtg
     1741 aaacaacggg gccactgatt tatatgattt tagccatgcc tgaagttgtg aaaaggaaag
     1801 agcattatta tagtgaaaaa aatacaaaag gagagatatt atgattccta agaagataca
     1861 ttactgttgg ttcgggggaa atccacttcc gaaaaaagat agaaagtgca tagaaagctg
     1921 gaaaaagtat tgccctgatt atgagattat tgaatggaat gaatcaaatt atgatttaaa
     1981 taagaaccgt tatatggccg atgcatataa agaacgtaaa tggggttttg ttccagatta
     2041 tgcaagattt gatattattt ataatcatgg tggattttat ttagatacag atgttgagct
     2101 aattaagtcg ttagatatac ttcgtgaaaa tcatgcctat atgggatttg aaggggaaat
     2161 ctgggttaat ggcggtattg gttttggtgc agaagaagga cattctgtta ttaaaggttt
     2221 aagggatatt tacgattcat taagttttta tcaaa
//
LOCUS       DACEXQ010000292         2254 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.295, whole genome shotgun sequence.
ACCESSION   DACEXQ010000292 DACEXQ010000000
VERSION     DACEXQ010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.295"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(231..611)
                     /gene="yajC"
                     /locus_tag="IXH04_002671"
     CDS             complement(231..611)
                     /gene="yajC"
                     /locus_tag="IXH04_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAQ1853219.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(708..1853)
                     /gene="tgt"
                     /locus_tag="IXH04_002672"
     CDS             complement(708..1853)
                     /gene="tgt"
                     /locus_tag="IXH04_002672"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAQ1853220.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            2071..>2254
                     /locus_tag="IXH04_002673"
     CDS             2071..>2254
                     /locus_tag="IXH04_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853221.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEA"
BASE COUNT          635 a          463 c          373 g          783 t
ORIGIN      
        1 gtaaacactt ttttgtgatt tttttcacag ttatttttaa atataaaatt gctttattta
       61 caggtgtttg tatagaaaaa tatataaagt tatttattat tatttatatg acacacccga
      121 ttattcaata ttgagcaata tatcccatgc tcttatctct ttcttttttg aataaaaaat
      181 gaggtaggac atctttctgc cctacctcgt ctttaaataa gctttgatac ttatttttct
      241 tccttctctt ctgtctcttc cagcgtttct gttgtttcag tttttacttc aactgtctca
      301 gctggttctt ctgttgtttc aacagtagtc gttgcatcat ttgttacgac agtccctggt
      361 ttgaccgttt tgattgctgc acgatcgtac tcaaggaaga tcccttcgca gtctaaaaca
      421 actgtccgtt tgtcattgtc gatttctgaa acaactccat gtaatccgcc gatcgttacg
      481 actgaatcac ctggtttcat gctatctaac aagttttgac gttcttgctg ttgtttcttt
      541 tgcgaacgtg acataaaaaa ccacattgct aatagcgcga taaacatcac aagcattggt
      601 aatgttccca tttttttcac ctcactaatt ctgtatctac aaaaaacact ttagcagaaa
      661 aatgctgata acactagttt tttacttaat ttttaaaaga ttttccttta gaagcttttt
      721 gcgttttctt tattgaaccc gtattcttca aaaaaggctt ctctaaattc aagcaaatta
      781 tcatccatga ttgcttgacg tacttgtttc attagattca acaagaagta taaattgtgg
      841 taagaagtca aacgaatccc aaatgtttca tcacatttga tcaaatggcg gatataggca
      901 cgtgtatagt ttttacatgt ataacaatca catttctcat caattggacg gaaatcatgg
      961 gcatatttcg catttttcac tactagacgg ccttgagaag tcatacatgt gccatttcga
     1021 gcgattcgag taggcaagac acagtcaaac atgtctactc cacggataac accatcgatc
     1081 aatgaatcag cagcaccgac acccatcaag taacgtggtt tgttttcggg gatcaacggt
     1141 gtagtgaatt ctaatacacg attcatttct gatttaggtt ccccgacaga taacccgccg
     1201 attgagtaac cagggaaatc catcccgact agatctttcg cgctttgacg acgaagatct
     1261 tcaaaccctg ctccttggat gattccaaat aatccttgac ggtctggatt agcatgagct
     1321 ttcaatcctc gttctgccca tcgagacgta cgttctactg attttttcac gtagtcgtaa
     1381 ctttcatcga atggtggaca ttcgtcaaaa ctcatcatga tatcagagcc caatttattt
     1441 tggatgttga ttgctttttc tggtgataaa aacatacgcg aaccgttcaa gtgatttttg
     1501 aaatgcacgc cttcttcttc gatattccgc atatcactta atgaaaaaac ttgaaagccg
     1561 cctgaatctg ttaaaatcgg ttgatcccag ttcataaatt tatgcagtcc gccagcttct
     1621 tctactaagt cttcaccagg gcgtagccat aaatgatatg tattacttaa aataacgcca
     1681 gcacccattt ctttcaactc ttctggggac attgttttta ctgtagctaa tgttcctact
     1741 ggcatgaaca ttggtgtggg gaaagttccg tgtggcgtga tcaattcccc taaacgcgcg
     1801 ccagtatgtt tttctttctt gattaaacga tagcgaatgg caggttgcgt cattcatttc
     1861 cctactttct atataaaata tcttgacaac tcccaccatc ataatcgttt caagtgcttt
     1921 ttgcaagttc cctcggcttc cttctacttt atttaatatt ctttttttta tccttttttt
     1981 atgaataaca aaaaaaagaa aaacattatg acagaaatat tttttgatac atggtatatt
     2041 ggtgttatta aagaaaagga gggaaaaatc atgcaatctt ctgcagaatt aaaacgccaa
     2101 gcaaaagaga gtctaaaagg acgttgggga caagctgttt tactaaatct aatccctaca
     2161 ttgattggta ttgctactat tcttcttatt atgattccta ttatcttgtt tgccaccacc
     2221 atatatgatg gatcagcgag cgtctcagaa gcga
//
LOCUS       DACEXQ010000293         2244 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.296, whole genome shotgun sequence.
ACCESSION   DACEXQ010000293 DACEXQ010000000
VERSION     DACEXQ010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.296"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(1014..1304)
                     /locus_tag="IXH04_002674"
     CDS             complement(1014..1304)
                     /locus_tag="IXH04_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853222.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1512..2203)
                     /locus_tag="IXH04_002675"
                     /pseudo
     CDS             complement(1512..2203)
                     /locus_tag="IXH04_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
BASE COUNT          637 a          426 c          328 g          853 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattac tgttgcttct ttactaattt aggtttactt
     1501 gttaagaaag attatttacg caaatgttct tcaacaattt gctgagattt atcagctgta
     1561 tcttccactt catcttttgc tttttctaac ttttgaatat attgtttcaa gcttgagttt
     1621 tcagcagttg cttcgtctaa tttcttttgt gtatctgtta actgatttct acatttgttt
     1681 tttcttgtgt cagtgttgcg atttgttgat tcaaaccatt tacagtattt tgtaattctg
     1741 aaagttttgc ttgtgcttct tgtagtcttg ctgtccaatc tgcattcaga tgattgattt
     1801 catcattttt ttggttgatc tcatctagtt tgacattgat ttcattttgt ttgctttgta
     1861 gttgtgcatc cttgctgact aattgacttt ctaagttttg tttttctgct gccgattgat
     1921 ttttttggtt ttctaattca tttacttttg tttggtattc ttggacatct gcctttgaat
     1981 cttgcaatgt ctgttctttt tgtgctagtc gagcggtaag atctgtaatc gcttgctctt
     2041 tatctgtcaa tgtatgatca agctgtgtca gattctcatt gattgtatca atatctgtct
     2101 ctccacccca caaaataatt tcatttgcgt accgcgtgcc tgtgtagcca attagacata
     2161 gaaatgcaac gacaatccca ccagtaataa atttcttttt catttgcata gctccttaat
     2221 ccaatacttt tacatgctaa aagt
//
LOCUS       DACEXQ010000294         2233 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.297, whole genome shotgun sequence.
ACCESSION   DACEXQ010000294 DACEXQ010000000
VERSION     DACEXQ010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.297"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            199..1149
                     /gene="yidC"
                     /locus_tag="IXH04_002676"
     CDS             199..1149
                     /gene="yidC"
                     /locus_tag="IXH04_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAQ1853223.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     IDVTESAKPADSKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(1328..1528)
                     /locus_tag="IXH04_002677"
     CDS             complement(1328..1528)
                     /locus_tag="IXH04_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853224.1"
                     /translation="MGGISALSFMENGVLCGRATWAKGVAPFVTEGEEKASSWMLEEG
                     KQNIKELSTLLEETATPVYVDR"
BASE COUNT          690 a          458 c          375 g          710 t
ORIGIN      
        1 ttaaagtaag aaagaaacac ttggaatcat tctacttttt cctagtattt ctttcttgat
       61 tttttgtctt tttttcaaaa tttttcgcta cgtaaaagaa cgaatattta tatgaaattt
      121 ctatgaagtt gattgaattc catgcgcttt tttagtatag ttaagtttgt gtaaaaatta
      181 tagaagagga agtattctat gaataaatgg aaaaactggt tattgggatc cggacttgtc
      241 agtatcctgc tctttttatc tggctgtgtg cggatggatt caaacggaaa ccctgacact
      301 tcgggaatcg tttatcgagt actcgtccat cctatgggcc aagcgattac atatctagtt
      361 gaaaatttca attggtctta tggctgggca gttattgcaa tgactgtgat cgtccgtatc
      421 atcatcttgc cactaggtat tagtcaatcg aaaaaaacaa tgatccaatc tgaaaaaatg
      481 caagcgttaa aaccacaagt tgaagcagcc caacaaaaat tgaaagctgc aacaacacgt
      541 gaagaacaaa tggcagcaca agcagaaatg cagcaagtat accgcgaaaa cggattgagt
      601 atgaccggcg gaatcggctg tctgcctcta ttgatccaaa tgccgatctt ctctgccttg
      661 tactttacgg cacgttatac agaaggtatc cgggaatcat ctttctacgg catcgattta
      721 ggctcaccaa gtatggtact tgttgccatt gcaggggtcg cttatctgct tcaaggatac
      781 atctcaacga tcggtattcc tgaagaacag aaaaagacga tgcgtacgat gctgatcgtc
      841 tctcctgcta tgatcgtctt catgtcgatc agtgcgccag ccggtgttac actttactgg
      901 gtagtcggtg gtgtgttcag ctgtctgcaa acattcatca caaacgttat gatgaaacca
      961 cgtttgaaag cacaagtggc agaagagttg aaacaaaacc caccaaaaca agtcgtcact
     1021 ccaagaattg atgtgacaga atcagctaaa ccagctgaca gcaaacaagc aaccaaacaa
     1081 atcaagcaac ccaaaaacag tacaaacaat aaaggccgca acgcaggaaa gcagcagaag
     1141 cggaagtaga tagtaaataa gagtgtaata aaaattcatt aacagtttta gcaattaaaa
     1201 atatcgacca agatttttga aaaagaaatc tggtcgatat ttttatttaa gtgaatgttt
     1261 aatttgaatc atcgattgat cgctatctaa ttgcagcagc atctttttat gaagaaacaa
     1321 ctttttctta tctatcaacg tagacaggtg ttgctgtttc ttctaatagt gtacttaatt
     1381 ccttaatatt ttgttttcct tcttctaaca tccaactact cgctttttct tcgccttcgg
     1441 taacaaaagg cgccactccc ttcgcccatg ttgctcgccc acataagaca ccattctcca
     1501 taaaggacaa cgcggagata ccacccatct aaataacatc cataaacctt tctagtagaa
     1561 gaatagtgat agtatccatc cctttttcta ctgggaatac aattacacga ttcaaagctt
     1621 gatacacaac atttcccttc ttataaaccg ctattgtaac cgaattgttt tgaatcacct
     1681 ttttgggata gaaactggct cctttccgca acttcttaaa tgttggtttc ttttatactt
     1741 tctttgtagc aactacaaat ttcttcaaat ctctaaatgt ttgcataaca accgcctttc
     1801 tgacttttac tcaggatggt atttaaaaat cagcaaaata aggcttctct ccatcttttt
     1861 tatttcctag ctgctttaaa gacactcttg ctgtttttta atctctatca agggggctct
     1921 gaatctattt tgatgaactt atcttattaa tttctcaaat gagctgtcag acacaactgt
     1981 attttttcaa tctcataccg gacaactatg ccagcttaaa aaaatttcct ctggcaaaac
     2041 ttctttattg aaaaatgaca atgaaaaaag accattcttt ttgttgaatg ctcattgtgt
     2101 aaacatattt cctgcgactg ctcttcgaac cattctcagt gcaatcccta atttatctat
     2161 taaatcttct tttagttcct catctttcat attcaaaatt tcctttgtat gttacacaag
     2221 tttgttctct aaa
//
LOCUS       DACEXQ010000295         2214 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.298, whole genome shotgun sequence.
ACCESSION   DACEXQ010000295 DACEXQ010000000
VERSION     DACEXQ010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.298"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(26..982)
                     /locus_tag="IXH04_002678"
     CDS             complement(26..982)
                     /locus_tag="IXH04_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853225.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
     gene            complement(1107..>2214)
                     /locus_tag="IXH04_002679"
     CDS             complement(1107..>2214)
                     /locus_tag="IXH04_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HAQ1853226.1"
                     /translation="HLEKAINTLLRTELTAFLDYEKYDRTGFNSGNSRNGSYFRSIKT
                     EYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLETTIIHLFEKGVTMSEIADLIEKMYG
                     HHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAIFMDATYIPLKRQTVSKEAIYIAIG
                     IREDGTKEVLSYAIAPTESTYVWNELLQDINSRGVQEVLLFITDGLKGMKDTIHQIYP
                     KAKYQHCCIHVSRNIAHKVRVKDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNY
                     PKVTQSLIENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLER
                     FLVSIFDTYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          666 a          369 c          375 g          804 t
ORIGIN      
        1 agtcggactt tctaaaagaa gttcactatt cgatcctgtc gatgatttct tcaggaaaat
       61 cgtctatttg cttgtcaatt ttgctgtctt tgtatttctc ttcttggata gtcaacaaaa
      121 tctttgtaag attcccccag tctactgctc ttggcggatc gagccaaacc actacttgag
      181 atgcactttc ttcatataga ttattcctat tcgtgattat aacatccggg aactcaggct
      241 ttcctgcctc tatcacttcc gtgttcagat taatgtacaa gcttaaattt ttcttgataa
      301 attgattata ggcagcacca taagaaaaat cgatgatgat ctttactggt tcagaaatca
      361 gttgaatggg tatgtgattg atcaataata ataaataact gaaaaaaaga aaaaatttat
      421 cttgaagcaa ctctttgcgt tttgacgtta gatcatttat atactctatc aaatagcaat
      481 aaaatgctgg atagttttct ctaaaaaaag aaacatcaag tggaaattca tcagctttat
      541 taatagtgta atgcaaaagt ttaaaatgta ttttggttaa ctctcttcgg atcaattggc
      601 tcgtctcttc tcgcaacaga ttaaactgat tttcaaaact ttcaatgaat cgttcattca
      661 accattcgat tgtttcatct tcattttttc tcaagtattt ttgatccaat ttatcatgaa
      721 gataaagcca agagagtgtc ttattgataa aaataacgga tttttctcct aatactcctt
      781 ttaagtggta aaatcctata tccgaaaaaa atagcgactg attgctagca atatctaaag
      841 aatcagcttg ttcagccaac aggttgattg ctaaaaagtg gaataatttc tttttttcat
      901 aagcagacag aggataatac tgttttaaat cttttaacat tttttctgtt tttataatta
      961 taggtagcgt atacaattcc acaaaactta tctctgttga gttaaaaaga ttatataaaa
     1021 aaagccgaat gtttttagga ttccctgtca acttgggagc gtcaataatt ttgtgtaaat
     1081 aaattgtcct cctgcaaaat aattagttac tcagtaaaca ttgaaactaa tgtatcggtt
     1141 acctgttgaa aacctttatg gcttctgttt agaaattttt gattgtatgt atcaaaaatg
     1201 ctgactagaa agcgttctag tgattcttca ttttgaaact gctcttttct acggctgtat
     1261 cttttaattt gcttattgaa agactcgatt agattggttg agtaaatggt tctacgaatg
     1321 ctaggtggaa aatcataaaa agttaataag tcttggtttt ctatgagtga ctgcgtcact
     1381 ttaggatagt ttttcttcca tttctcaatc atgccggata agaaggtatt cgcttcttct
     1441 tttgagttag cttgataaac agccttaaag tcatcacaga tttcttttcg gtctttgaca
     1501 cgtactttat gagcgatgtt acgagataca tggatacaac aatgctgata ttttgcttta
     1561 ggataaattt gatggatagt atctttcatg ccttttaagc cgtccgtaat aaaaagcaag
     1621 acttcttgaa ctcctctgga gttaatatcc tgtagcagct cattccaaac gtatgttgat
     1681 tcagttggag caatcgcata actcagtact tctttagtgc cgtcttctcg tataccaatg
     1741 gcaatataaa tcgcttcttt ggatacggtt tgacgtttta gtggaatgta agtagcgtcc
     1801 ataaaaatag cgacatactt atcatttaag gctctggatt taaaggcatt tacttcttca
     1861 gtcagaactt tagtcatgtt ggacatggtt tgtggagtat agtgatgacc gtacattttt
     1921 tcgatcaaat cagcaatttc agacatcgta acaccttttt cgaataaatg gataatagtg
     1981 gtttccaatg tatcgtttgt tcttttgtag gctggtaaag tttgttgttt aaactcacca
     2041 ttacgatctc taggtatttc caatgttaat tcaccatatt cggttttgat tgatcgaaag
     2101 taagaaccgt ttctcgaatt acctgaatta aaaccagtgc gatcatattt ttcgtaatct
     2161 aaaaaagccg ttaattcagt ccgtaggagt gtgtttatcg ctttttctaa gtgc
//
LOCUS       DACEXQ010000296         2197 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.299, whole genome shotgun sequence.
ACCESSION   DACEXQ010000296 DACEXQ010000000
VERSION     DACEXQ010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.299"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            278..802
                     /gene="infC"
                     /locus_tag="IXH04_002680"
     CDS             278..802
                     /gene="infC"
                     /locus_tag="IXH04_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAQ1853227.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            874..1074
                     /gene="rpmI"
                     /locus_tag="IXH04_002681"
     CDS             874..1074
                     /gene="rpmI"
                     /locus_tag="IXH04_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAQ1853228.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            1127..1486
                     /gene="rplT"
                     /locus_tag="IXH04_002682"
     CDS             1127..1486
                     /gene="rplT"
                     /locus_tag="IXH04_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAQ1853229.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            1838..>2197
                     /locus_tag="IXH04_002683"
     CDS             1838..>2197
                     /locus_tag="IXH04_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAQ1853230.1"
                     /translation="MATVWMGLALVGNTFSPNSAMEGQGVIDVLTHIYTTGPAEYAKS
                     ILVNVFFGAFFGRVLVDSGIAATLIRKVVELGGDKPRITMSLLCIVTAVIFMSMTGIG
                     PVISIAVIVLPILMSLGI"
BASE COUNT          736 a          384 c          479 g          598 t
ORIGIN      
        1 aatcactgct agaaagcctg ctaatctaat cattttcaga aaaggaagac aaactgtatg
       61 aaaaacattg acaaaaagat tagtttttgc tatactataa tggttgagaa ataaaagcag
      121 aagcacccgc ttctcgcctt agttgataat gtcactgggc tagatagttt tcactggttc
      181 tattaggtga agtacgcgcg ggttctgtaa gagaactcgt ttttttattg ccgtttattc
      241 ggtaagcttt tctttctctc ataatatttg gaggtgaatg accatagcaa aggatatgat
      301 ggttaacgac ggcattcgtg cacgagagtt acgattgatc ggttcagatg gtgagcaatt
      361 aggagttaag acaaaagcag aagcattgaa tattgcagaa caagcaaact tggatcttgt
      421 gttagttgcc cctggtgcga aaccaccagt tgcgcgaatc atggactacg gtaaataccg
      481 tttcgaacaa caaaagaaag accgcgaagc gcgtaaaaaa caaaaagtga tcaatgtaaa
      541 agaagttcgc ttaagtccta cgatcgactt gaatgacttc aatacaaaac ttcgtaatgc
      601 acgtaagttc ttggaaaagg gcgataaagt gaaagcttct atccgtttca aaggccgtgc
      661 cattacccat aaagaaattg gtcaaaaagt cttgaatcgc ttagctgaag aaactgcgga
      721 tattgcaaca gtggaacaaa aagcgaaaat ggacggacgc agcatgttct tgacgctggc
      781 accgaaaaac gacagtaagt aatttgaatg actaactggc tgttggacag ccgatagtcg
      841 gataacgaaa gattttgagg aggaaatata gtcatgccaa aacaaaaaac acaccgcgga
      901 tcagcaaaac gtttcaaacg tactggtaaa ggcggattga aacgcttccg cgcgtttaca
      961 agtcaccgtt tccacggcaa aacaaaaaaa caacgccgtc aattgcgtaa agcaagcatg
     1021 gtttctagtg gcgacttcaa acgtattcgc caacaactag caaaaatgaa ataagaagta
     1081 caaataacag aatctgaaga tttattttta ttaggaggaa ttatacatgg cacgtgttaa
     1141 aggtggaaca gtaactcgta aacgtcgtaa aagagtgcta aaattagcaa aaggttacta
     1201 cggttcaaaa cacactttat ttaaaacagc aaaagaacaa gtaatgaatt catacaacta
     1261 cgcatatcgc gatcgtcgtc aaaagaaacg cgacttccgt aaattgtgga ttgctcgtat
     1321 caacgcagca gctcgtatga atggcttgag ctactcaaaa ttaatgcacg gtttgaaagt
     1381 tgctgaaatc gacatcaacc gtaaaatgtt agctgacttg gctgttaacg atgcagcagc
     1441 atttactgca ttggctgacc aagcaaaaga agctttggct aaataagcta aacaattata
     1501 aagaggaaag aagataacga aaagttatgt tctttcctct ttttttattt acaatcctcc
     1561 atcaaatgaa acaggacaat aacgtataag tctaaaataa cataattaag gttttttaat
     1621 tgaaaactga acatttatgg tcatccttta atgaaagcgc taactacata agttagaatg
     1681 agtttgactg atatattagt ttgcttatca gaaaaacaaa atggacggag gaataacatg
     1741 agtgatatta ttattggtat tttgttagtt atctcattct ttttcatggt ctggtactgt
     1801 gtaaaaggct acaacttgat ggtcggcttt tgtcatcatg gctactgtat ggatgggatt
     1861 agcgctagtc ggaaacacgt tttcaccaaa ctcggcgatg gaagggcaag gagtaattga
     1921 tgtgttgacg catatctata cgacggggcc agctgagtac gccaaatcga tcttggtaaa
     1981 tgtcttcttt ggtgcctttt tcgggcgtgt tctagtagat agcggaatcg cagctaccct
     2041 gattcgaaag gtagttgaac ttggagggga caagccaaga atcacgatga gcttactatg
     2101 tattgtaacc gctgttatat tcatgtcgat gacaggaatc ggaccagtga tttctattgc
     2161 agttattgtg ttgccgatct tgatgtcatt aggtatt
//
LOCUS       DACEXQ010000297         2191 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.300, whole genome shotgun sequence.
ACCESSION   DACEXQ010000297 DACEXQ010000000
VERSION     DACEXQ010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.300"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            353..1081
                     /locus_tag="IXH04_002684"
     CDS             353..1081
                     /locus_tag="IXH04_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ1853231.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRTNESDYNKRDQVLL
                     VKLNEEISVEEAVRTFNASDMVNYAEPNYVVELEPLNDVEEPNTTDSLGNRSIILNDP
                     NWCKQANLREISMPSAWEETKGSKDVKVAVIDSGIDYQHEDLSGNVDESLGYDFFDDD
                     TDPMDTMGHGHILPGLLVLKQTTN"
     gene            1111..>2191
                     /locus_tag="IXH04_002685"
     CDS             1111..>2191
                     /locus_tag="IXH04_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HAQ1853232.1"
                     /translation="MVPIRVFGSSGGTSLAILTEAINYASQKGFPIINFSIGSVVYSS
                     TEKAIKNYPGLFVAAAGNNGIDMTDKPFYPASLDIPNIITVGNSSGYERSSDSNYRKT
                     GVDLFARGFRVFSTFLGSKYTYMSGTSMATPHVTGSAALALSLDPDLSTDELKKIILN
                     SVDSFYELSDLCLTGGRLNTNNVVRQVTGKLTASNITLRLNSEWSDEIAKEMCHVHWI
                     DQSGNDLTDQVVVLKNEVDTSNFGDYEVVFASPDLTDQISVTVTVPRLRKLTLGKETA
                     TIDIGSAWDSVIAIDTFQVKAIDDFGNDVTNQVQVTINADIDTINSIQAVIKFEVPEL
                     GLAQIGRLNITAKNSTINGLNEVSIE"
BASE COUNT          769 a          290 c          470 g          662 t
ORIGIN      
        1 cgtggagaaa taataagaac taccagtcag ataaaacaag ataccgaaga ctcactgcgc
       61 aaagggtggg gaatgaataa taaagttaga taataatcaa agaagaacta agtaactacc
      121 tttaaacggt agttacttag ttcttctttg atttgcgtga aatagagggg atttattttt
      181 ctggttacgt gattaaaaaa tccaaaaaat gagcatagga acggttatga ttttagggtg
      241 tgttttaatg agatattgtg atttcattaa caagtgtatg ataatctgtt gttgagttag
      301 taaataagta atagagctaa ctctaaaata aaactattag gaggagatgt aggtgaaaaa
      361 gtttaaaaag aaaatatatt taggattggt tgttggggtg agtatgtttg ggttcatggc
      421 tcatactaaa caagtaactg cagatactaa ccaaaaagaa aggattatga gccaattaga
      481 tgactctcaa atagagtatg tacccaatga gctaattgtc agtttaaagg ctacacaaag
      541 aatttcaaca gagaaaaata aaccgcaaaa tttattgggt gtaaaagtta tggtggaaaa
      601 agatttgact cttaggacaa atgaaagcga ttataataag cgagatcaag tattattagt
      661 gaaattgaac gaagaaatta gtgtggaaga ggcagtaaga acatttaatg cttctgatat
      721 ggtcaattat gcagagccaa actatgtggt tgaactagag ccgttgaatg atgttgagga
      781 gccaaataca actgattctt taggaaatcg atcaattatt cttaacgatc ctaattggtg
      841 taaacaagct aatttaagag agattagcat gccatccgct tgggaagaaa ccaagggttc
      901 taaggatgtg aaggtagcgg tcattgattc aggaattgat tatcaacatg aagatctttc
      961 tggaaatgta gatgaaagtt tgggatacga tttttttgac gatgatacag atcctatgga
     1021 tactatggga catggacaca tattgccggg attattggtg ctcaaacaaa caacgaatta
     1081 ggtgttgctg gaataaattg ggatgttacg atggtaccta tacgtgtatt tggtagtagt
     1141 ggaggtacca gtttggcaat cttaactgaa gctattaatt atgcatccca aaaagggttt
     1201 ccaattatca attttagtat aggtagtgtt gtttatagca gtacagaaaa agcaattaaa
     1261 aactatcctg gattattcgt agcagcagct ggaaataatg ggatagatat gactgataaa
     1321 cctttttacc cagcttcttt ggatatacct aatattatta ctgtaggaaa ttctagtggt
     1381 tatgaacgaa gttctgattc aaattatagg aaaacaggtg ttgatttatt cgctagaggg
     1441 tttcgtgttt ttagtacatt tctaggaagt aaatacacgt atatgtcagg tacctcgatg
     1501 gcaacacctc atgtcactgg tagtgctgca ttagcgttgt cactagatcc tgatctatct
     1561 actgatgaat taaaaaaaat cattttaaat agtgttgatt cattttatga actatctgat
     1621 ttatgtctta cagggggaag actaaatacg aataatgtcg tacgacaagt aacgggtaaa
     1681 cttactgcat ctaatatcac attaagatta aatagtgaat ggtcagatga aattgctaaa
     1741 gagatgtgtc acgttcactg gattgaccag tcaggaaatg atttgacgga tcaagtggtt
     1801 gttctaaaaa acgaagtgga tacatctaac tttggcgatt acgaagtagt ttttgcatca
     1861 cctgatttaa cagatcaaat ctcagttaca gtgaccgtac ctcgattacg taaacttacg
     1921 ctaggaaaag aaacagcaac cattgatatt ggttccgcat gggatagtgt catagccatt
     1981 gatacttttc aagtgaaagc tatagatgat tttgggaatg atgttacaaa tcaagtacag
     2041 gttactatta atgctgatat agatacaatt aattcaattc aagcagtgat taagtttgaa
     2101 gttccagaat taggcttagc ccaaattggt agactgaata ttactgcaaa aaatagtaca
     2161 atcaatggat tgaatgaagt tagcatcgag c
//
LOCUS       DACEXQ010000298         2187 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.301, whole genome shotgun sequence.
ACCESSION   DACEXQ010000298 DACEXQ010000000
VERSION     DACEXQ010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.301"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..261)
                     /locus_tag="IXH04_002686"
     CDS             complement(<1..261)
                     /locus_tag="IXH04_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853233.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSRE"
     gene            complement(271..1431)
                     /locus_tag="IXH04_002687"
     CDS             complement(271..1431)
                     /locus_tag="IXH04_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAQ1853234.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            complement(1431..2117)
                     /locus_tag="IXH04_002688"
     CDS             complement(1431..2117)
                     /locus_tag="IXH04_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAQ1853235.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
BASE COUNT          605 a          475 c          329 g          778 t
ORIGIN      
        1 ctcacgcgaa tgatcttctt ctaaatattt ccaccataaa tatggtttaa tcgaaggaat
       61 atactctttg tcgtcccgca gatcatcgta cacatgaact tcaacaccat attccttctc
      121 aaaaaaaata ggaatctgat catcgtgtct gctgaaaagc aatacgatat ctttgattcc
      181 tagtttcttc agattagtta aacaaacttc aagctcccat ttaaaccgat tgattgccgg
      241 ctgacaaaga atatacttca ttctgatcac ctacgcttgt gttgtagttg ttgtggttgt
      301 tggttttgta gttgtagtag tagttcccaa agcgctaata tctaatacaa tgaaactatc
      361 gttacgttta ggttgaccgt ttgcatattg tttagctaga taaatgcgtt cgtcttcaac
      421 aaaatggtat tcatctgaag cttcaatttt tagtgtagat cctacaccca tgaagtaatc
      481 tgaggctacc ccaataactg cttttccttc tggcacagcc gttgactgca aatctgaaac
      541 tggtactggc aatacttgta cgtattctcc attagcagtt agtacagtct tagctgggaa
      601 tactttagac cagtaatcag ttggattcac aattaggacc acatcagaag gattcacatt
      661 acgataaatc ggatcattca caccttcgat attgaatttt gatagtcgcg ccatcaaacc
      721 gcccatagtt acagcatcta aagctgtaat aggttctgct tttttttcag catattctcc
      781 gctagtttgt ttgctcatgt cacgcatcat tccgactggc atatctttac cagtaccatc
      841 aacaattgct tgttctaatg caattctcaa tgattctact aaaacagtac ggacataacg
      901 atctaaccat actggaccta aatcaagcat tgccttacat acaggaatat aacctgatag
      961 cttgaactgc ttcatgttaa ttacatcaaa gccattatct aaaacttttt taacagcttc
     1021 gcaaagttta ccccaccatg ctggattgac tccacgtgac acaatccatt ctgttacacc
     1081 agttgtgtta acaaaagtaa ttttttgcaa tagtggatga gattgttcta aatcttcaaa
     1141 tacacgttca aatacagtag ctggcactaa ttcttcgacc cctgcaaaac cttcgttttt
     1201 cactacttcg ttatagaatt ttgtttcttg tgtagttaat acacgctgac cacggttcat
     1261 taatactaat tgatcttgat tttttgctgt tgcttcttct aaaattttat cctgaatttc
     1321 cttagataag cttaccatag ctgcgctaaa agattcttcg ttaccatctt taaaagcttt
     1381 catcaattgg tcgcttgcag ctgttacacc ttttaaattt ttaactgtca ttattttaca
     1441 tctccttgtc caaatgtttt atttaatgct gctgtaaatg cagcaatttt ttctgctctt
     1501 ttttctttaa cgtcattcaa aatttcttca acgctttgtt cttttttagc ttcagtacct
     1561 gagctatttt ctgcatcgat aatttcatcg accaatccat aactcaaagc tgtttctgca
     1621 tccataaacg attctttttc aagaagttct tgcaatgctt catctgtgcc attgaatcgt
     1681 gttttatatg aagcctttac cgatttatca attgattcca gttggtcagc aatcgtacgg
     1741 aagtcatcga catttccttc tccgtatgtg gaagcgcggt gaatcatcaa ttgtgcattg
     1801 ttgtagattt ttatagtatc gccagccatt gcgataattg aagcagcact agcggctaag
     1861 ccgttaatca caacgttaac ttttgcttta tttgacttaa gtaagttccc aatagcaatc
     1921 ccttgaaata cgtctccacc gtttgaatta attactactt caatttcttc ttgatcacct
     1981 agactatcca aaatattttt gattcccttg tcagtattcc cttcaaagaa ccaactagaa
     2041 ccaataaatc cctgaataaa aatttgcggt actgcgcctt cattctttac tgctagaaat
     2101 gttttcattg tcgtcattcg cctcacctcc tttcgatact tgttgattgt ttttagttat
     2161 aaatatttca tctgccatcg ccttatc
//
LOCUS       DACEXQ010000299         2156 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.302, whole genome shotgun sequence.
ACCESSION   DACEXQ010000299 DACEXQ010000000
VERSION     DACEXQ010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.302"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            983..2068
                     /gene="xerS"
                     /locus_tag="IXH04_002689"
     CDS             983..2068
                     /gene="xerS"
                     /locus_tag="IXH04_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAQ1853236.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
BASE COUNT          785 a          358 c          310 g          703 t
ORIGIN      
        1 tagtcatggc atgttttatt gtgctggttc atcaatatat tgaaatgtac taaaatgaaa
       61 ttttcagtta tggtataata tttatgctgt ctgctacccc ggcctattat ggcaagggga
      121 ggaggttgta ttatgaatta ccttttcgat atgttttttg caatcttagc agaagtatcc
      181 gcgcattaca tctgtaaatg gcttgattgc aaaattattg ccaaaaaaga cagtaagcct
      241 acccgttaat ctgaatagaa cgtaaaagaa aaccactagc ccgccagcta gtggtttttg
      301 gtgtatcatg aattaccttt tcgttaaaac aattatacca tatttcctaa aaaatacaat
      361 taatagacga attatcatta ccactttgat gaaaaaagct gacataaatt tttcttttat
      421 ttttataatg aataaacatt tatcaataag cttttgaaag gcaggatcgt actttgttga
      481 tgattgagat attgctacat caatctcagc agttaagatt tttttgaatt gttgcttgaa
      541 ttttttcttt atctaaaaaa attgtatctc caggatgcaa cccattttct ttaagaaatt
      601 ccttttaaat tgttaatact actgagtgcc ccacttctct gaattttctt taacttctcc
      661 ctcccctttt ttatttgtat atacattcat atacgcttgt taatatcaaa tcaaccgtag
      721 tctctcttat gtctaattat atagaaagat tgttctttct atataattta gacaatttaa
      781 aaaattaaga ttgcctcctt ctgtttcctt tcctattcat gtataccatt tattttaaat
      841 tattaaatct atacctattt atctgctttt ttgaaataaa ttaataaatt tcagagtata
      901 ccaattgaca aaacactcta taattggtat agtccaatta tagagatact cctctcccct
      961 acttatcgaa aggatgaata acatgaaaaa tacagaaata tatcaaaaaa tagatacaat
     1021 catacttcat atgcccgatt atataaaaga ttttgttcaa gatcgtgagg ataaagatca
     1081 atcacctcga actactttag aatatctaaa aaattataag ctcttttatg aatggcttct
     1141 gagtgagtct atcgttcccg atacttcaat cacctcgata agacagttaa ctgcctatga
     1201 tctaaacttg tacaaatctc atttgaaacg tcgcacaaag gaaaatgtaa aaaaagagac
     1261 aacaaaaact aagctcaaag ataataataa cttaggtcta tctacttcta ctatcaatag
     1321 aaatatcaca gcattaaagg ttctatttaa atatctttcg aaaagttcca ataatccttt
     1381 aggaaaacca tacttagaaa ataatcctat ggatcaagta gcaactatca cagataaaac
     1441 tacgttagct gcccgtgcaa acgcaatcga gaaaaaatta tttctagacg aagatacaca
     1501 aaattatcta gattatattg ccaatgaata taaaaaaact ttgagcaaac gagctttgat
     1561 ctattatcaa cgagatgtag aaagagacct ggcgatcaat gcattgattt taggatctgg
     1621 attaagactc tccgaggttg taaatattaa tttagaggac ctatctttag aaaagaataa
     1681 tgtggtagta actagaaaag gaaataagag agacgcagta aatatagcag catttgcaat
     1741 ggaatattta gctaattatc tagctattcg gaaagaacga tacaaagtaa cagaaaacga
     1801 aaaagcttta tttctagcaa tttatcaagg agaagccaag cgtatatccg gtattgcaat
     1861 cgaacggatg gtagcaaaat attcaaaagg attcagagtc caagtcagtc cacataaatt
     1921 acgtcatact ttggctacta gactctatca acagacaaat tcattggtat taactgccca
     1981 acaattggga catacaagta caaatacaac aactttatat actcatatag acaatgcagc
     2041 tacaattgat gctttgaacg ctttgtaaat gtagtaaaaa atagtataat aatgaagcct
     2101 gtctctaaaa ttagataaaa gcccaacttt aaaggcaagc ttcattatca ttttta
//
LOCUS       DACEXQ010000300         2151 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.303, whole genome shotgun sequence.
ACCESSION   DACEXQ010000300 DACEXQ010000000
VERSION     DACEXQ010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.303"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(137..1666)
                     /gene="ahpF"
                     /locus_tag="IXH04_002690"
     CDS             complement(137..1666)
                     /gene="ahpF"
                     /locus_tag="IXH04_002690"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAQ1853237.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(1676..>2151)
                     /gene="ahpC"
                     /locus_tag="IXH04_002691"
     CDS             complement(1676..>2151)
                     /gene="ahpC"
                     /locus_tag="IXH04_002691"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAQ1853238.1"
                     /translation="GNWSILCFYPADFTFVCPTELEDLQEQYSTLKSLGVEVFSCSTD
                     THFTHKAWHDTSDAIGKIEYVMIGDPSHQISRIFDVLDEEQGLAQRGTFIIDPDGIVQ
                     AMEINADGIGRDASALIDKIRAAQYVRTHPGEVCPAKWKESGETLKPSFDLVGKI"
BASE COUNT          637 a          458 c          372 g          684 t
ORIGIN      
        1 ttcaaattat ttactactta tagtatataa gaaaaagaga agaaaaagcg attattcaaa
       61 aaaacaaaaa ggattgtgac aaaattcttg tcaccatcct tttttccgct ctctgacttt
      121 ttacaatctt catagctcaa gtgcgcatca gataatcgaa tgctcctaaa gctgcagtag
      181 ctcctgaacc cattgaaaca atgatttgtt tgtatggact atctgtacaa tcaccagctg
      241 cgaaaacgcc ggggatattc gttgcgccat ttttctcggt cactatttca tttcttggat
      301 tcatttcgat cgttccatct agccaatccg tattagggag taagccgact agaatgaata
      361 ctcctgaaac agctagtgta tgcatctcca tcgtacggcg atcgatatag gagagagatt
      421 ctacctgatt cgtaccgctg atttctttcg tcgctgcatg tttgataaca gtgacattcg
      481 caagagagac tagtttatcc tgtaagattt gatctgcttt taattctggt aagaattcta
      541 gaacatatac atgttttgct aacccagcca aatcgattgc tgcttcaaca ccagaattac
      601 caccaccgat aacagcaatc tcttttcctt taaataaagg gccgtcacag tgtgggcaat
      661 aagcaattcc tttattttta aactcttttt cgcctggtac attgatagaa cgccagtgcg
      721 cacctgtcgc gatgatggcg gtttttgctt taagtgatat accgttagct aagccaatat
      781 taataaaatg ttgctcttta tttaaggaaa cggcctgttg attcatcata atgtcgatag
      841 ggtaactacg cacatgactt tctacttgct gcatcaattt tggaccttct gtgtaagagg
      901 taccaatcat attttcaatc cctagtgttt caacgacttg tccgccgaaa gaatcagcga
      961 caagtcctgt acggattcct ttacgtgctg catagatagc tgcactacta gctgctggtc
     1021 caccgccaat cactaataca tcataaggtt ctttttcaaa caactcttct tcaatcaaag
     1081 ggccagtgat tttttcaaca atttgttcga tcgtcatgcg tccgctacta aattcttctc
     1141 catttaaaaa cacagttggc actgccatga tttttttatc atcgatttct ttttggaaca
     1201 tcccaccttc aatcatcgta tgagtaatat ttggatttaa tacagctaat atattcaatg
     1261 cctgtacaac gtcggggcaa ttatgacaag tcaagctaac ataactgtca aaatgcaatg
     1321 tttgtgttat ttttttgatt ttaagactca aagattcact gattttcggt gcccgtccac
     1381 taatttgtaa catagccagt aaaaaagagg aaaattcatg accgaggggg ataccagcaa
     1441 aaacgattcc actttctttg ccaactggat tgatagaaaa actaggtgtg cgcatcgaat
     1501 ctcttttttc ccaagagatt ttaggagaca tttctgctat ttccttcgta aattcagcta
     1561 gtttttgact attttcacta ttatccaaac taacagaaaa aacaatagga gattcaatca
     1621 gtgctaaata ttgttcgaga ttttgtttag tttctttgtc taacatatcc accacctaaa
     1681 tctttccaac aagatcaaaa cttggtttca atgtttcgcc actttctttc cattttgccg
     1741 gacaaacttc acctgggtgg gtacgtacat attgagcagc gcgaatctta tcaatcagtg
     1801 cactagcgtc acgaccgatt ccatctgcat tgatttccat agcttgaacg attccgtctg
     1861 gatcaataat aaatgttcct cgctgagcta atccttgctc ttcatccaaa acatcaaaaa
     1921 tacgagaaat ctgatgagac ggatcaccga tcatcacgta ttcgatttta ccgatagcat
     1981 ctgatgtatc atgccatgct ttgtgagtaa agtgggtatc ggtagagcaa gaaaagactt
     2041 caacacctaa agatttcaat gtactgtatt gttcttgtaa gtcttctaat tcagtaggac
     2101 aaacaaaagt aaaatcagct ggatagaagc aaagaatact ccagttcccc a
//
LOCUS       DACEXQ010000301         2144 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.304, whole genome shotgun sequence.
ACCESSION   DACEXQ010000301 DACEXQ010000000
VERSION     DACEXQ010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.304"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            35..1336
                     /locus_tag="IXH04_002692"
     CDS             35..1336
                     /locus_tag="IXH04_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ1853239.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
     gene            complement(1434..1784)
                     /locus_tag="IXH04_002693"
     CDS             complement(1434..1784)
                     /locus_tag="IXH04_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ1853240.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(1778..2041)
                     /locus_tag="IXH04_002694"
     CDS             complement(1778..2041)
                     /locus_tag="IXH04_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HAQ1853241.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
BASE COUNT          670 a          428 c          343 g          703 t
ORIGIN      
        1 gaatcaccac aacacaatca aaggagcgtt ctgtatgcct gatcttcatc atatcaaact
       61 actacttgga atcaaagata aaaacatttt catcacaaat gtggaatcga agagcattaa
      121 gaatatcaag agtcttgttg tttctgcgac gttaagtaaa gaaattcgtc gctgtccttt
      181 gtgtaaacag atgaatcatg aaggcatgat cgttaagaac ggaaagaaaa aatcgctcat
      241 tcaacttaat aaatgtgcga accaactgac ttatcttgct ttagcgaaac aacgctacca
      301 ctgtcgtggc tgccacacct attttacggc gaatacctac attgtcgata gaaactgctt
      361 tatcgctaaa caagtgcgct ataaaatttt agaagagcta actgaaaaac aagcaatgac
      421 gacaattgct aagcattgcg gcgtttcttg gtcgaccgtt tctcggacac tcgcttctct
      481 gatccccatg acaaaagtaa aaagaaattg gttaccgcgt tgtttattaa tggatgaatt
      541 tcgttcactg aaaaatcaag tcggtcccta ctccttttct tgtatggatg gcgatactgg
      601 aaaattactg gatattcttc cttcacggaa gaaaaaggct ctcgtttctt attttatgca
      661 atttgaacga cgcgcacgat tgaaggtaaa ggtcatcgtg acggacatga atgtttctta
      721 tgcctctctg ataaaggaat gttttccaaa ggcaaaactc gttgtggatc gttttcacat
      781 tgtcaaacat ctcattcgta aatttgagga tattcgcgta cgaatcatga aaagctttga
      841 tcgaaacgat ccaacacaag ccaaatatta tcgacaatta aaggcgttgt ctcggcttct
      901 gatcaaacga caggacaagc ttgtttatga caaatggacc aaatggcgca attttggttg
      961 ggcgtatctc accgaaagtg aagtggtgga tcgtctgctt tcgatctctg acgaattacg
     1021 aatcgcctat ttttactatc aagaaattct tcaagcgttt cacgacaaag aggcggacac
     1081 tttctttaaa cttgtaagaa cgatgcccaa ttccgttcca aaagaactgc attctataaa
     1141 aaaagctttt ataaactatg aatccggcat tcgtttagcc ctcgaattgc cttattcaaa
     1201 tgccaaaata gaaaatctgc atacccacat caaagcattg aaacgagtcg cttatggctt
     1261 tcgaagcttt cggaaaatga agaccagaat tttcttacta aataatctga ttacctatga
     1321 atcaaaaaac atctaaagta gaaaaggaat tctaccttag atgttcttat ctcactctat
     1381 cagtcctatt tgacaaagag ccacgaatag ttgatcatgc tgctgtttct attttatttg
     1441 cttacttttt gaaaaactgt tctaactttc atcaataact cctcaactaa actatcatgc
     1501 gctttttcca aaaaaataca ctctctggct tcataatcaa ttgtcgttag ttgatctaat
     1561 aatacttttc ctgttgtctt catttcatat tctgagttta tatttacata tagcggatag
     1621 tttctagttg tactagaaat tggagcaact gtaacaacgt tactataatc cgcaataata
     1681 tcatgactta aaacaatata aggtctttta cctgtctgtt cgtgtccaga tcgaggtgct
     1741 gtattcaata atagaatatc gccttgtcta ggttttctca ccatttttca ttacctactg
     1801 cttcacctaa attttgaatc gtcacatgat ttctttcatc agtgtaattt tcaaataact
     1861 gttcaattgt taattcattt tctatattag ccgcttcgat aattagccgc ttctgaccgt
     1921 ttacttcttc ttgtattatc ttcactttgt cttctgtatg aattccaaga tattccataa
     1981 tatactttgg caatctaagg ccattactat ttccccattt ttttataggt aacatttcca
     2041 tagtagacac ctctttctgt atatacatag tataacatac ataagaaaaa acgtatatac
     2101 ttaattatcc ttttctatta aattagatta acattccatg aatc
//
LOCUS       DACEXQ010000302         2119 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.305, whole genome shotgun sequence.
ACCESSION   DACEXQ010000302 DACEXQ010000000
VERSION     DACEXQ010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.305"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..1380
                     /locus_tag="IXH04_002695"
     rRNA            <1..1380
                     /locus_tag="IXH04_002695"
                     /product="23S ribosomal RNA"
     gene            1474..1589
                     /gene="rrf"
                     /locus_tag="IXH04_002696"
     rRNA            1474..1589
                     /gene="rrf"
                     /locus_tag="IXH04_002696"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            1603..1675
                     /locus_tag="IXH04_002697"
     tRNA            1603..1675
                     /locus_tag="IXH04_002697"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1636..1638,aa:Val,seq:tac)
     gene            1679..1751
                     /locus_tag="IXH04_002698"
     tRNA            1679..1751
                     /locus_tag="IXH04_002698"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1712..1714,aa:Lys,seq:ttt)
     gene            1759..1840
                     /locus_tag="IXH04_002699"
     tRNA            1759..1840
                     /locus_tag="IXH04_002699"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1793..1795,aa:Leu,seq:tag)
     gene            1858..1930
                     /locus_tag="IXH04_002700"
     tRNA            1858..1930
                     /locus_tag="IXH04_002700"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1891..1893,aa:Thr,seq:tgt)
     gene            1942..2013
                     /locus_tag="IXH04_002701"
     tRNA            1942..2013
                     /locus_tag="IXH04_002701"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1974..1976,aa:Gly,seq:gcc)
     gene            2030..2115
                     /locus_tag="IXH04_002702"
     tRNA            2030..2115
                     /locus_tag="IXH04_002702"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2064..2066,aa:Leu,seq:taa)
BASE COUNT          554 a          468 c          621 g          476 t
ORIGIN      
        1 atgcttcttt tcttaaggac aagctgtgat ggggagggaa ataatagtac cgaagttcct
       61 gatgtcacac tgccgagaaa agcttctagt gagaaaacag cggcccgtac cgcaaaccga
      121 cacaggtagt cgaggagaga atcctaaggt gagcgagaga actctcgtta aggaactcgg
      181 caaaatgacc ccgtaacttc gggagaaggg gtgctgatca tacgatcagc cgcagtgaat
      241 aggcccaagc gactgtttat caaaaacaca ggtctctgca aaatcgtaag atgaagtata
      301 ggggctgacg cctgcccggt gctggaaggt taagaggagt gcttagcgca agcgaaggta
      361 cgaattgaag ccccagtaaa cggcggccgt aactataacg gtcctaaggt agcgaaattc
      421 cttgtcgggt aagttccgac ccgcacgaaa ggcgtaacga tttgggcact gtctcaacga
      481 gagactcggt gaaattttag tacctgtgaa gatgcaggtt acccgcgaca ggacggaaag
      541 accccatgga gctttactgt agtttgatat tgagtgtctg taccgcatgt acaggatagg
      601 taggagccgt agaaatcgga acgctagttt cgatggaggc gctggtggga tactacccct
      661 gcgttatggc cactctaacc cgcaccacta atcgtggtgg gagacagtgt cagatgggca
      721 gtttgactgg ggcggtcgcc tcctaaaagg taacggaggc gcccaaaggt tccctcagaa
      781 tggttggaaa tcattcgaag agtgtaaagg cagaagggag cttgactgcg agaccaacaa
      841 gtcgagcagg gacgaaagtc gggcttagtg atccggtggt tccgcatgga agggccatcg
      901 ctcaacggat aaaagctacc ctggggataa caggcttatc tcccccaaga gtccacatcg
      961 acggggaggt ttggcacctc gatgtcggct cgtcgcatcc tggggctgta gtcggtccca
     1021 agggttgggc tgttcgccca ttaaagcggc acgcgagctg ggttcagaac gtcgtgagac
     1081 agttcggtcc ctatccgtcg cgggcgttgg aaatttgaga ggagctgtcc ttagtacgag
     1141 aggaccggga tggacttacc gctggtgtac cagttgttct gccaagggca ttgctgggta
     1201 gctatgtagg gaagggataa acgctgaaag catctaagtg tgaagcccac ctcaagatga
     1261 gatttcccat ttctttaaga aagtaagatc cctgagagat gatcaggtag ataggtcagg
     1321 agtggaagta cagtgatgta tggagcggac tgatactaat cgatcgagga cttaaccaat
     1381 aggttaaaag aaaagaactc ggaaaaaaac ttatgtgact tcaaatccag ttttgagtga
     1441 acaaaattta ctcaataaaa gataacacca cagtgtggtg gcgatagcga gaaggataca
     1501 cctgtaacca tgccgaacac agaagttaag cttcttagcg ccgattgtag tgaggggttg
     1561 ccccttgtga gagtaggacg tcgccacgct taagttatcc atggaggttt agctcagctg
     1621 ggagagcatc tgccttacaa gcagagggtc agcggttcga tcccgttaac ctccatatga
     1681 gccgttagct cagttggtag agcatctgac ttttaatcag agggtcacag gttcgagccc
     1741 tgtacggctc atatttttgc gggtgtggcg gaattggcag acgcactaga tttaggatct
     1801 agcgccttac ggcgtggggg ttcaagtccc ttcacccgca taatttaagt tttacatgcc
     1861 ggcttagctc agttggtaga gcatctgatt tgtaatcaga gggtcgaggg ttcaagtcct
     1921 ttagccggca tctttaaaca cgcggaaata gctcagtggt agagcaccac cttgccaagg
     1981 tgggggtcgc gggttcgaac cccgttttcc gctttgccaa agcaatcgcg ccggggtggc
     2041 ggaactggca gacgcacagg acttaaaatc ctgcggtgag tgatcaccgt accggttcga
     2101 ttccggtcct cggcatttc
//
LOCUS       DACEXQ010000303         2118 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.306, whole genome shotgun sequence.
ACCESSION   DACEXQ010000303 DACEXQ010000000
VERSION     DACEXQ010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.306"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..341)
                     /locus_tag="IXH04_002703"
     CDS             complement(<1..341)
                     /locus_tag="IXH04_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAQ1853242.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIED"
     gene            491..973
                     /locus_tag="IXH04_002704"
     CDS             491..973
                     /locus_tag="IXH04_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAQ1853243.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            1010..2020
                     /locus_tag="IXH04_002705"
     CDS             1010..2020
                     /locus_tag="IXH04_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAQ1853244.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
BASE COUNT          736 a          330 c          432 g          620 t
ORIGIN      
        1 ttatcttcaa tattaaatga aatcaattgt tcaatcaatt cccagttgat tacacttttc
       61 caaggaactt tgatgacgtt atccgtatat acgtagcctg cttcttggat atttttttca
      121 agggcacgaa tcgctgcttt ttccggtgcg atagaaaaat gatttttagc aaaactaaat
      181 gcaataataa aggtaccatg gtcagtaaac ataggttggt tccattttac agttgtgtct
      241 agctggggat acgtttctgt tatcttagca aaaaggtctt tgatcttttg ttgatgttct
      301 ttttctttga tttgattgat gatcggctga aatttttcca ttctaaaacg cctccattaa
      361 gtggtttgat ttattttacc agaaaaacaa tagttatgta taaaaaacga acaaaacaca
      421 agtaatgttg taaccgctat atttatatga taagatgtct caaataataa aatagaaaag
      481 agacataaaa atgattgact tggcaaagaa attcttacac gaaaatatat attttgagtt
      541 gtatcatcat cgagccattt atacaaatga agatgctttg gtaataaaga aagaacaagg
      601 atttacaggt acagagacca aaagcttatt cttaaaagat aaaaaagata ataactacgt
      661 atatctgact tttactacga aacaaacgga tttcaaagca ttagggaaga tcgtcggtaa
      721 aaggctttct attgtttcat cagaacgcat ggaagaacaa acgggacaaa agcagggcgc
      781 tgtttctccg tttggttatg aaaaacagat tccgattatt attgatgaag agctcttatc
      841 aaatgaaaaa cttgtttttg caccaggtag accagatcaa acgatggtca tccaaacggt
      901 tgacttaaac aaaatcatcc agctacttga gatgccgata tttttgatgc cggcaaacga
      961 aaaaaacaat taaccaagta aaatagtgca tacttggagg tgaagcaaca tgataagtta
     1021 tccattatta gaaagaaatg caacgattgg ggttactgca ccatcctcag gcgtatcaga
     1081 ggaacaccat tcgttactcg aatcagcttt aaagagaatg gaagaaaaat ctttccaagt
     1141 agttactgga aagacgcttt ggacgcaaga taaggaaaaa tcaggtccag caagaaaacg
     1201 agcagatgaa ttcaatcaaa tgatgcaaga tgaaaatatc caaatgatct ttccgacttg
     1261 gggaggagaa ttattgattg agattctcga ttttgtcgat tttgatgctc tacaaccaaa
     1321 atggatagta ggttattcag atattagtac gttgctactt gctgtcacat taaaaacagg
     1381 gatagccact gcacatggaa ctaaccttat tgatttaaga ggagagtact tggatgaaac
     1441 gacagcaatg tgggaaagtg tactgaaaac aagagaagga gaatcgattc tacagcaatc
     1501 ttctgaaaag tatcaaaaag tgtggcccca tgagcattca tcatcttctg ttttccatct
     1561 cactgaacca acaaattggc aatcagtcag cggagatgca gtaaggatga agggacgatt
     1621 attaggtggt tgtattgata cgatcaggca tttaatcggt actccttatg gagatgtcca
     1681 atcttttaaa agaaagtata atgatggaaa gccgatcttg tggtttctag aaaactgtga
     1741 gctatcgtca gttgatttaa gaagatcact tgttcagatg aaactggcag gctggtttga
     1801 tggcagctcg gggatcatgt tcggtagaag tgcggcagag gatttgttag attataaaat
     1861 gaaggatgta tacgaagatc tgtcaaagga attaaaacta ccgatcttgt atgatattga
     1921 ttgtggtcat gtaccgccac aactgacatt gattaatggt gcctatgcag aagtagcggt
     1981 agaagataga aaagcgactg ttttgcaaac gtttatttaa aaaatttttt cctgccttgg
     2041 agtctatcaa agaccaataa atctaaatag aaaagaaagt gaaagaagct gacaaaatgc
     2101 tgtcagcttc tttttgta
//
LOCUS       DACEXQ010000304         2043 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.307, whole genome shotgun sequence.
ACCESSION   DACEXQ010000304 DACEXQ010000000
VERSION     DACEXQ010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.307"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..356
                     /locus_tag="IXH04_002706"
     CDS             <1..356
                     /locus_tag="IXH04_002706"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="With PurL and PurS catalyzes the conversion of
                     formylglycinamide ribonucleotide, ATP, and glutamine to
                     formylglycinamidine ribonucleotide, ADP, and glutamate in
                     the fourth step of the purine biosynthetic pathway;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HAQ1853245.1"
                     /translation="SLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDE
                     ETLQELKKNNQIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGK
                     RFFASILKNFGKVTV"
     gene            357..>2043
                     /gene="purL"
                     /locus_tag="IXH04_002707"
     CDS             357..>2043
                     /gene="purL"
                     /locus_tag="IXH04_002707"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAQ1853246.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMV"
BASE COUNT          659 a          378 c          488 g          518 t
ORIGIN      
        1 agtctttgca ttttatttgc aaaactgtac cattgaaagt caaccccaag acggccttta
       61 cttcagagta tgaagccgac gaaataatct atcttccagt tgctcatggg gaaggaaatt
      121 atttctgtga cgaagaaaca cttcaagaac taaaaaaaaa taatcagatt gcctttactt
      181 ataatgacaa catcaacggc agcgtggaaa acattgcagg gattacgaac aaagcaggga
      241 atgtgttagg aatgatgcct catccagaac gtgcagtaga agacttgcta ggatcagaag
      301 atgggaaacg gttctttgct tccattttaa aaaacttcgg aaaggtgacc gtatagatga
      361 aaacattgaa agaaccgaca gctgaagaga tcaagaaaaa aagaatctat gccgaatggg
      421 gattgactga tgaagaatat cgaatgatcg aagaagatat cttaggaaga atgcctaatt
      481 atacggaaac tggactgttt tctgttatgt ggagcgaaca ttgttcctat aaaaattcta
      541 aaccggttct acgcaaattt cctactagtg gacctcatgt gttgcaaggc cctggagaag
      601 gcgcaggcat cgtcgacatc ggtgatggac tagcagttgt cttcaaagca gaaagtcaca
      661 atcatccttc agccgtggag ccttatgaag gagctgcgac aggagttggc ggaatcatca
      721 gagatatttt cagcatgggg gctcgtccaa ttgcgttgct agattcattg aggttcggcg
      781 aattagataa tccaagaacg aaatatttac tagaagaagt agttgcaggt atcggcggat
      841 acggcaattg tatcggcata ccgactgtag gcggcgaaat tgcatttgat ccttgttatg
      901 aaggaaaccc attagtcaat gcaatgtgcg taggtttgat tgaacacaaa catatccaga
      961 aagggcaagc cgctggcgtc ggtaattcga tcatgtatgt aggtgcaaaa acaggtcgcg
     1021 acggtatcca cggagcaact tttgcctcag aagaatttaa tcaagaagaa gaacagcaac
     1081 gttctgctgt acaagtaggc gatcctttta tggaaaagct gctgcttgaa gcgtgtttgg
     1141 aattaatctt gcatcattcg gattctctaa taggtataca ggatatggga gcagcaggac
     1201 ttgtttcatc tagtgcggaa atggcttcaa aagctggctc aggacttata ttgacattag
     1261 atgaagtccc acaaagagaa actgggatga caccttatga aatgatgtta tcggaatcac
     1321 aagaacggat gttgatttgt gttaaatcag gagaggaaga aaaaatccaa gaactatttc
     1381 agaagtatga tctagatgca gtcactatcg gtaaagtaac agacgacggg caatatcggc
     1441 tatatcatca aggagaagaa gtagcaaatc tgccagtcga tgcattagct gaagatgcac
     1501 cagtttatca taaagaaatg aaagaaccag ctcgaatcgt agaatttcag cagttagctc
     1561 cttatcagcc agttatcgaa gaacctggac aagtattgct tgatctgctg cagcaaccga
     1621 cgattgcttc aaaacgttca atctacgaaa cttatgattc acaagtacaa accaatacag
     1681 ttgtccgtcc aggaagcgat gctgctgtga tgagggttag agggacgaat aaagcgttag
     1741 ctatgaccac agactgcaac gcgcgctatc tgtatttgga tccaaaaatc ggaggacaaa
     1801 tagcagtggc agaagctgct agaaatatta ttgctagcgg tggtaagccc ttggcgatca
     1861 ccgactgcct gaactatggt tctcctgata aaccagaagt tttttgggaa ttatcgactt
     1921 cagctgacgg aattgctgct gcgtgtgaaa cgttaggaac tcctgttatt tcaggaaatg
     1981 tctctttata taatgaaacg gatggtcaag caatctatcc aacaccaatg attggtatgg
     2041 tcg
//
LOCUS       DACEXQ010000305         2016 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.308, whole genome shotgun sequence.
ACCESSION   DACEXQ010000305 DACEXQ010000000
VERSION     DACEXQ010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.308"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..293
                     /locus_tag="IXH04_002708"
     CDS             <1..293
                     /locus_tag="IXH04_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAQ1853247.1"
                     /translation="NLKPSPYYITDYLESIGLVRDNSEQIINGLHIYPTDYFAAKDYD
                     TGKIYKTSNTISIHQYNASWKTPKQKFVYRLKSTIGQKKFNRLLALKKFLHL"
     gene            308..1393
                     /locus_tag="IXH04_002709"
     CDS             308..1393
                     /locus_tag="IXH04_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ1853248.1"
                     /translation="MKKICFISGVISRSGGTERVGSIIANSLSDLGYDVTILSFWNSG
                     EPYFGLDKTIKVDYMLNPKTEGKLYRTYLYPVWKLHKYICKNEFDIVIDIDTLLSLYT
                     TRAIKGTKCKLISWEHFNYWTMEMLKEKKRFKAKKLIKKYAKRLVVLTEEDRQKHIDE
                     YSLSEDFVITMPNPCISNIEYEYHYDKKRFLAVGRLVEEKNFADMISAWKIVEKKVDD
                     WDLIIVGKGELEKDLRQQIKDLNLQRVTIAGHSDHVEEYYKSASCYVLSSVYEGFPMV
                     ILEAQSYGLPVISYDCKTGPRDLVHHNFNGMLVEDKNIDQLAKSMIMFTKNTDLAMRM
                     SLNAYNNVQKFNLKEITKQWVALIENV"
     gene            1529..>2016
                     /locus_tag="IXH04_002710"
     CDS             1529..>2016
                     /locus_tag="IXH04_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ1853249.1"
                     /translation="MIINRKQNAKRNIITGTIFKLYQIIIPFFMRTLMIYQMGMEYAG
                     LNNLFTSIFQMLNLAELGIGAALTVSMYKPIAEDNRKEICALLNAYKKYFSIIGWIVF
                     TLGLLCIPFLRFLVKGSIPGNLNMTVLYLMYLINTVLSYWLFSYRKSLIYAHQRNDVI
                     NVS"
BASE COUNT          744 a          222 c          380 g          670 t
ORIGIN      
        1 tgaacttgaa accgagtccg tattatatta cagattatct tgaatctatt ggattggttc
       61 gcgataacag cgaacaaata ataaatggcc tgcatatcta tcctacggat tattttgctg
      121 ccaaggatta tgatactgga aaaatttata aaactagtaa tacaataagc attcatcaat
      181 ataatgcatc ttggaaaaca cctaaacaaa aatttgttta tagattaaaa tcaacaattg
      241 gacaaaaaaa atttaacagg ttacttgcac tgaaaaaatt tttacactta taaaggagaa
      301 aagtactttg aagaaaattt gtttcattag tggtgtaatt tctcgttctg ggggaactga
      361 aagagtaggg agtattattg ccaatagctt gtctgattta ggttatgatg tcactatttt
      421 aagcttttgg aatagtggag agccttattt tgggttagat aagacgatta aggttgatta
      481 tatgttaaat cctaaaacgg agggaaaatt atatagaact tatttatatc cggtttggaa
      541 attgcacaag tatatatgca aaaatgaatt tgatatagta attgatattg atactttgtt
      601 gtctttgtat actacaaggg caattaaagg aacaaagtgc aaattgattt cttgggaaca
      661 ttttaattac tggacaatgg aaatgctgaa agaaaagaag agattcaagg ctaaaaaatt
      721 gataaaaaaa tatgcaaaga ggcttgtggt tctcacagaa gaagatcgtc aaaaacatat
      781 tgatgaatat agtttatcag aagattttgt tataactatg ccaaatccgt gtatatctaa
      841 tatcgagtac gaatatcatt atgataaaaa gagatttctt gcagtagggc gattagtgga
      901 agagaaaaat tttgctgaca tgataagtgc ttggaaaatt gtagaaaaaa aagtagatga
      961 ttgggatctt atcattgtag gaaaaggtga attagaaaaa gatcttcgcc agcagataaa
     1021 agatttaaat ctgcaaagag taactatagc agggcattca gaccatgtgg aggaatatta
     1081 taaatctgca tcttgttatg tgcttagttc tgtttatgaa ggatttccta tggttatatt
     1141 agaagcacag tcttatggat taccagttat ttcttatgat tgtaaaactg gtccgagaga
     1201 tttggtacat cataatttta atgggatgtt ggttgaagat aagaatatag accaattagc
     1261 taagagcatg ataatgttta ctaagaatac tgatttagca atgagaatgt cactgaacgc
     1321 atataataat gtacaaaaat ttaatttgaa agaaattaca aaacaatggg ttgctttgat
     1381 tgaaaatgta tagttttttg tggttgtcca aacatgaaaa ttagcgtaat taatcaaaga
     1441 gctagaagaa acctattaca tttcaagatt ttagaaatta tatagtatta ggtaacaata
     1501 aaaaatagaa agtttggagc catatgatat gataattaat cgtaaacaaa atgctaaaag
     1561 gaatataatt actggaacaa tttttaagtt atatcagatt atcattccct tttttatgcg
     1621 tacacttatg atttatcaga tgggaatgga gtatgctgga ttgaacaacc tgttcacatc
     1681 tatatttcaa atgttaaatt tagcggaatt gggaataggt gctgcactaa ctgtaagtat
     1741 gtataaacct atagcagaag acaatagaaa agagatttgt gctttactga acgcatataa
     1801 aaagtacttt tcaattattg gatggatagt gtttacatta ggattgttat gcattccttt
     1861 tttgagattt ttagttaaag gttcgattcc tggaaattta aatatgacag ttttgtattt
     1921 aatgtatttg ataaatactg ttttgtcgta ttggttattt tcatatagaa agagtctaat
     1981 atatgcacat caaagaaatg atgtaattaa cgtgtc
//
LOCUS       DACEXQ010000306         1997 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.309, whole genome shotgun sequence.
ACCESSION   DACEXQ010000306 DACEXQ010000000
VERSION     DACEXQ010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.309"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            2..247
                     /locus_tag="IXH04_002711"
     CDS             2..247
                     /locus_tag="IXH04_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853250.1"
                     /translation="MIKKLIQFSMDLYDIESGATVSVESDHLIISFADERQIIIWVVD
                     DMLYPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSK"
     gene            244..642
                     /locus_tag="IXH04_002712"
     CDS             244..642
                     /locus_tag="IXH04_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853251.1"
                     /translation="MIPKFRAWDKRENTMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVEIFEGDVVITSKGAIGYVAYLQQEAGFVVVLKKSDYRLG
                     HRNTGESYDVATAHEVIGNIYENSELLEVE"
     gene            639..977
                     /locus_tag="IXH04_002713"
     CDS             639..977
                     /locus_tag="IXH04_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853252.1"
                     /translation="MIILATDYIDSLKDENGNVPALLNCGIKSKNRIILLRISADLAK
                     YLYLSKTAVYYTAATRYQYKGKTISQDYYDRLISPDMHGKSKSAIKLFGAIEIYADDF
                     PNLWLKEEAE"
     gene            974..1204
                     /locus_tag="IXH04_002714"
     CDS             974..1204
                     /locus_tag="IXH04_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853253.1"
                     /translation="MKYEIPLSEAGIQAIINGREVNKKLPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSINDTFRLGS"
     gene            1211..1414
                     /locus_tag="IXH04_002715"
     CDS             1211..1414
                     /locus_tag="IXH04_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HAQ1853254.1"
                     /translation="MNTRHRRVAKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASETIRKFGISILNDDRKWEAER"
     gene            1411..1704
                     /locus_tag="IXH04_002716"
     CDS             1411..1704
                     /locus_tag="IXH04_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853255.1"
                     /translation="MKLKDGFYTDAYGIGGLMMDLPTKNPIKQEESEIKVGDMVRCEA
                     EGFIYPFRGYVEHLYNHSAIIRIENTMECDKRLAKSKENLAVVRLVDMEVIKS"
     gene            1734..>1997
                     /locus_tag="IXH04_002717"
     CDS             1734..>1997
                     /locus_tag="IXH04_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1853256.1"
                     /translation="MSVIPQLEGEEAIVNILENIDIKQTRKNARRLLKRYRNLERLVG
                     PVKIDFSVMTVTKNLKFTIDSQNEEIIEAISTRDSVIEALTRLS"
BASE COUNT          737 a          280 c          444 g          536 t
ORIGIN      
        1 aatgataaaa aagctcattc aattcagcat ggatttatat gatatcgaat caggagcaac
       61 agtatctgtg gagtctgatc atttaatcat aagttttgct gacgaacgtc aaattatcat
      121 atgggtagtt gacgatatgc tatatccaga aattgtacat gattttgaag aatcaaaagc
      181 ggttgagttt gaaatagtga aaaaagtaat ggaattgatt gaaaaatacg aggaggacag
      241 caaatgatac cgaagtttag agcgtgggat aagcgagaaa acacaatgag agatgtagct
      301 gtcttacatt ttactaaaaa cggcaaaaca aactttattg aatattggat aaatcctacc
      361 gaattgaaat catatcacgt gcgaaacatc gacctcatgc aatccacagg aatgaaagat
      421 aagaatggtg tggagatatt tgaaggggat gtagtaatta catcaaaagg agcgattgga
      481 tatgttgcgt atcttcaaca agaagccggc tttgtagttg tactaaagaa aagtgattat
      541 agattaggcc ataggaatac tggcgaaagt tatgatgtag caactgctca tgaagtcatc
      601 ggaaatatat acgagaatag cgagttattg gaggtagagt gattatctta gctactgact
      661 acatcgattc tttaaaagat gaaaatggga atgtcccagc attgctaaat tgtggaatta
      721 aaagcaagaa tagaatcatt ttgttacgga tatcggctga tttagcaaaa tatctttatc
      781 tgagcaagac agcagtatat tacactgcgg ctacacgtta tcagtacaag ggaaaaacaa
      841 tcagtcagga ttattatgat cgtttaatca gtcctgacat gcatggaaaa tcaaaatcag
      901 cgatcaagtt atttggcgca atagagatat atgccgatga ttttccaaat ttatggttga
      961 aggaggaagc ggaatgaaat acgaaatacc actaagtgaa gcgggcattc aagcaattat
     1021 caatggtcgg gaggttaata aaaaacttcc tgatggtact gaattagtca tcagacaaag
     1081 ttatttgaaa gatatggcag ctccagtatt aattgatcgt tttaacgtga ctgattctgt
     1141 ggtagagaac cacttgaaag aatttcgatc aagtataaac gacactttca gattagggag
     1201 ttgattgaca atgaacacca gacaccgcag agtagcaaaa ctaagaaaac aggaactgaa
     1261 tgtactaaag acaaagtttg aaaaagaata tggaatttca gcagaagaaa catataaagt
     1321 ggcaagtcag tttgttgctg atgcgagtga gactattcgt aagtttggga tttcgatatt
     1381 aaatgatgat cgtaaatggg aggcagaaag atgaaactaa aagacggatt ttacactgat
     1441 gcttatggaa ttggtggttt gatgatggat ctaccaacta agaatcctat aaagcaagag
     1501 gaatcagaaa ttaaagtcgg tgacatggtt cgctgcgaag cagaagggtt catctatcca
     1561 tttcgtggat atgtagagca tctctataat cactcagcga tcattcgcat tgaaaacacg
     1621 atggaatgtg acaagcggtt agcgaaaagc aaagagaatt tagcagtggt gagattggtg
     1681 gatatggaag ttataaaatc ttaattttgc ttttttacaa ctatcctaca acaatgagcg
     1741 ttattccaca actggaagga gaagaggcaa ttgtgaacat tttagagaat atagatatta
     1801 agcaaacaag gaaaaatgct agacgattac taaaaagata cagaaattta gaacgtttgg
     1861 tcggaccagt gaaaatagac ttttccgtga tgactgttac caaaaatttg aaattcacaa
     1921 ttgacagtca aaacgaagaa atcattgaag cgataagtac tcgagattcg gttatcgaag
     1981 cattaacacg gctaagt
//
LOCUS       DACEXQ010000307         1989 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.310, whole genome shotgun sequence.
ACCESSION   DACEXQ010000307 DACEXQ010000000
VERSION     DACEXQ010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.310"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            161..391
                     /locus_tag="IXH04_002718"
     CDS             161..391
                     /locus_tag="IXH04_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAQ1853257.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            488..1789
                     /gene="murA"
                     /locus_tag="IXH04_002719"
     CDS             488..1789
                     /gene="murA"
                     /locus_tag="IXH04_002719"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ1853258.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1802..1978
                     /locus_tag="IXH04_002720"
     CDS             1802..1978
                     /locus_tag="IXH04_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ1853259.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
BASE COUNT          632 a          328 c          414 g          615 t
ORIGIN      
        1 tttgtctcag tccctttttt tgcatatttt tagcatttat ttcctaaaaa atgtaagggg
       61 attcctatat ttttaagtat acttgttttt ccttttatac attttatggt attatcattt
      121 tattagttat tataaaacaa tcgttagaaa ggacgtcatt atgcaagtat acggaattga
      181 tgcggtgctt cgaatcgtga gtcatttggc gtttatctac ttagccttct ggtctttacg
      241 ttcgttacgc attgaaactt ttttcagacc aatgcataat acgcaaatta gaatggcaat
      301 cgtacttttt tcaatttttt tagggtacac tgcaagtaat tttttcttag aagttatcgc
      361 tttatgccga aatcttttcg ttagcctgta aattttaaaa aaaggtacta ttttaaaagc
      421 aaggatgtta taatagaaaa gattttgagt aagtgtatgc tcattggaat tttttggagg
      481 gaacaacatg gaagagatca tcgtaagagg tggcaatcaa ttaaatggaa cagtaagaat
      541 tgaaggagct aaaaatgctg ttttgcctat tttagctgct agtttgttag cagaagaagg
      601 tattacgaca ttagacaacg ttccgattct ttctgatgtc ttcactatga atcaagtgat
      661 tcgtcactta aacgtagatg tagattttga tgaacaaaag aatcaagtaa caatcgatgc
      721 ttcaagacaa ttagaaatcg aagcgcctta tgagtatgtt agtcaaatgc gtgcttcaat
      781 cgttgtgatg ggtccgctac ttgccagaaa tggacatgca aaagtagcta tgcctggtgg
      841 atgtgcgatt ggtaaacgac caatcgattt gcatttaaaa ggatttcaag ctttaggagc
      901 aaaaattatt caaaaaaacg gctatatcga agcaattgcg gatgaattga tcggtaacac
      961 gatttattta gatttcccta gtgtaggtgc tactcaaaat atcatgatgg ctgccgtaaa
     1021 agcaaaagga acaaccatca ttgaaaacgt agctcgcgaa ccagaaatcg ttgatttagc
     1081 aaacattttg aataaaatgg gcgctcaagt ctatggagct ggtactgaaa caatgcgtat
     1141 tgaaggcgtg gatcatttac atgcagtcaa ccactcaatc gtacaagatc gtatcgaagc
     1201 aggtaccttt atggttgctg ctgcaatgac acaaggcaat gtgctgattg cagatgctat
     1261 ctctgaacat aaccgcccat tgatttctaa attgatcgaa atgggtgcag aaatcattga
     1321 agaagaaggc ggagtccgtg tcatcggacc aaaacatatc ttacctacag atgtaaaaac
     1381 gatgcctcat cctggtttcc ctacagatat gcaagctcaa atgacagcta tccaattagt
     1441 tgcagaaggt acaagtgtag tgactgaaac agtgtttgaa aatcgtttcc aacatttaga
     1501 agagatgcgc cgtatgaacg ctcatgtcaa aatcgacgga aatgtagcaa tcatggatgg
     1561 aaaccatgaa ttgcaaggtg cagaagtata tgcaacagac ttgcgggcag cagcagcatt
     1621 agttttagct ggcttgaaag caaatggaat cacacgtgtg cgtaacttga attatctaga
     1681 ccgtggatat tataatttcc atatcaaact acagcaatta ggtgcagacg ttgaacgtgt
     1741 agacatggat caaacttctg ctgaaaaaac agcacaaacg attgcttaag gaagtggctg
     1801 aatgagtaac caacgatata tcttattaac tttgataaag attttagtgg tgatcttatt
     1861 gcttatcctt ctttttgtgg ccggaacgat gatcggatat ggtgtcatcg gtggcggcaa
     1921 tccatttaaa gtattccagc caagtttgtg gattcatatt cgagactttt ttcattgata
     1981 aataagaat
//
LOCUS       DACEXQ010000308         1979 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.311, whole genome shotgun sequence.
ACCESSION   DACEXQ010000308 DACEXQ010000000
VERSION     DACEXQ010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.311"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(325..528)
                     /locus_tag="IXH04_002721"
     CDS             complement(325..528)
                     /locus_tag="IXH04_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853260.1"
                     /translation="MSQLGKCYSLSKSNRKVYTLFISDSVKEGFTLGFSISFKKGTKN
                     TSISHNNRELTDSQKINGLSGRK"
     gene            complement(728..1744)
                     /locus_tag="IXH04_002722"
     CDS             complement(728..1744)
                     /locus_tag="IXH04_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAQ1853261.1"
                     /translation="MANENQLSEIQSKIEGRGAEKRTTINQLEKLNRISNRRLSVQGT
                     TTPLKLNWSIDRITIVGKLNDTIAYWTKNGVVFVDFEQLMRLNENQGYLKSVGINGWQ
                     LFDQYGEDIAYIEILKFHEGKGRIDFNPNKIGQFLASSMKGFIHDLFLDPHFSRADIA
                     CDIVDVPDDFITQYRIVDPISFKPIYGRSGKLETAYWGSRASERQVRLYNKKLEQERK
                     KVIVPKEIDTWWRLEMQLRRGKATDWHAMVQESLDSFASPHFLPIDIKPIDKIVIDGL
                     IAEPSNWSIIARHTKYKYRNLLKQESQNDELTNHLRETFKESADELKKELDTWLLGLD
                     VTEK"
BASE COUNT          557 a          459 c          309 g          654 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatggaaa gggcgcaccc gctgtttatt tcattcagct atcggttttc acaatctaac
      121 acgatcgttc cgctaccagt cgccttacag gctccatttt cgctcctcta acgtgtttta
      181 acgattgtgc cgataactaa acgaaataaa cgctaaaacc gctcagaacg caaaatatgc
      241 cattttagag ctattgtaga aaatgtagtt tgaaatgcaa ttttcttctt tcagcttttt
      301 atttcccact tgtaaatctg ctaattattt tctaccagac aaaccattga ttttttggct
      361 atcagttaac tcacgattat tatgagaaat gcttgtattc tttgttccct ttttgaacga
      421 aatactaaac cctaaggtga acccctcctt cacgctgtcg ctaataaaaa gagtgtaaac
      481 ctttctgtta ctctttgaca aagagtaaca ttttcctaat tgtgacatgg ctgtcacaat
      541 tccaaatgct ctccgagggt attcgctgtc acaagtgaca acgaaaaaag gagccgaaag
      601 cgtgtcacgc tctcaactcc ttttacgctt tattttgctt catgcaaaat attcgcttat
      661 cgtgaattta cgtcactaaa cattgacgca aatccactct cactcatctg acttttctta
      721 ttcggtatca cttctctgtc acatccaacc ccaaaagcca cgtatccaat tcttttttta
      781 attcgtctgc agattcctta aaagtctctc gtaaatgatt cgttaactca tcattttgac
      841 tttcttgctt cagcaaattc cgatatttat attttgtatg tctagcaatt atcgaccaat
      901 tagaaggctc agctatcaaa ccatcaataa ctattttatc aataggtttt atatcaatcg
      961 gcaaaaaatg cgggcttgca aaactatcta agctttcctg aaccatcgca tgccaatccg
     1021 ttgctttgcc acgtcttagc tgcatttcta accgccacca cgtatcgatt tcttttggca
     1081 cgattacttt ttttcgttcc tgctctagct ttttgttgta cagtcgcact tggcgctcac
     1141 tggcacgact gccccagtac gctgtttcta gcttgccaga acggccgtag attggcttaa
     1201 aagaaatcgg gtctacaata cgatactgag taataaagtc atccggcaca tctacaatat
     1261 cgcaggcaat atccgcccgg gaaaaatgcg gatctaaaaa taggtcatga atgaatcctt
     1321 tcatggaact agctaaaaac tggcctatct tattcggatt aaaatcaata cgaccttttc
     1381 cttcatgaaa tttaagtatt tcgatatacg caatatcttc tccatattga tcgaataatt
     1441 gccagccgtt tatcccaact gatttcaaat atccctgatt ttcgttcaaa cgcattaatt
     1501 gctcgaaatc gacaaaaaca actccatttt tcgtccaata agctatggta tcattcagtt
     1561 ttccgacaat tgtaattcga tcaatcgacc aatttaattt taggggggtg gtcgtacctt
     1621 gcacacttaa ccgcctgtta gaaatacggt taagtttttc gagctgattt atcgttgttc
     1681 ttttttctgc ccctcgacct tcaatttttg attgaatttc actcaactga ttttcatttg
     1741 ccattttcgt cctccaaacg aaaaaagaga cttttggaaa gaatttccag agttcacgta
     1801 accctaaaaa ttcatatcca aaaatctcta gcttgaagaa acctaaaaat attcgtataa
     1861 tgaacctgag ttacggagct aggtgaagta gtttccgtaa atagtcgaga gtgttagcgc
     1921 actgctcggc ttttattttg ctcttttttc tcgttttctt tgattatagc actcctcct
//
LOCUS       DACEXQ010000309         1955 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.314, whole genome shotgun sequence.
ACCESSION   DACEXQ010000309 DACEXQ010000000
VERSION     DACEXQ010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.314"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            201..1166
                     /locus_tag="IXH04_002723"
     CDS             201..1166
                     /locus_tag="IXH04_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAQ1853262.1"
                     /translation="MSKNGTIVNEWENLTALSNRRLNVKTFYTPLKIRWSIDRITIVG
                     KLKENIYYHTQNDVLILDFEQLMRLNENNGYVKSVGLNGWQIVDKHDENIAYIEILKY
                     QEGQGRIDFNPNKINQFLASSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQVRMYNKKLEQERKKAVVPKEIETWWRLELQ
                     LRRGKATDWHKMVHESLDSFASPHFLPIDTKHTDRIMIAGLMSDHSLWSGLTRPTKYK
                     YRNLLKQESQNDELTNHLRETFSESADNLKQELDTWLLGLDVTEK"
     gene            1366..1568
                     /locus_tag="IXH04_002724"
                     /pseudo
     CDS             1366..1568
                     /locus_tag="IXH04_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352749.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          645 a          293 c          433 g          584 t
ORIGIN      
        1 aaaaaaatag ccgattggtg ttggaagcac ctttcgacta attacggaaa ctatcgcact
       61 agctccgtaa ctcataatca ttatacgaat atttttaggt ttcttcaaga ctagagattc
      121 ttgttaatga attttaaggg ttacgtgaac ttttaaaatt ctttccaaga atctcttttt
      181 tctttgagga ggaacgaaat ttgagtaaaa atggcacgat tgtaaatgag tgggaaaatt
      241 taacagccct atctaacagg cggttaaatg taaaaacttt ttacaccccc ttgaaaatac
      301 ggtggtcaat tgaccgaatt accattgttg gaaaactgaa agaaaatatt tattatcata
      361 ctcaaaatga tgtattgatt ttagactttg aacagttaat gcgactgaat gaaaataatg
      421 gttatgttaa atctgttggt ctaaatggtt ggcaaattgt ggataagcat gatgagaata
      481 ttgcttacat tgaaattttg aaatatcaag aagggcaagg gcgaattgac tttaatccga
      541 ataaaattaa tcagttttta gctagctcga tgaagaattt tatccatgat ttatttttgg
      601 agccgcattt ttcacgtgca gatattgctt gcgatataat tgacgtacca gatgatttca
      661 ttacccaata tcgggtggtt gatcctgtat cctttaaacc aatttatggt cggtctggca
      721 agttagaaac tgcttattgg ggcagtcgtg ctagtgaacg tcaagttcgt atgtacaata
      781 aaaagctgga acaggaacga aaaaaagcag tcgtgccaaa agaaatcgag acttggtggc
      841 gtttagaatt acagttgcgc cgaggaaaag caactgattg gcacaaaatg gttcatgaaa
      901 gtttggatag ttttgctagt ccacactttt taccaataga tacaaaacat acagatagaa
      961 ttatgattgc tggtttaatg tcagaccatt ctttatggtc ggggttaact agaccaacta
     1021 aatacaaata tcgaaatttg ttaaaacaag agagtcagaa tgatgaattg acgaatcatt
     1081 tacgagaaac attttcggaa tcggcagaca atttgaaaca agaattagac acgtggcttt
     1141 tggggttgga cgtgacagag aagtgatacc gaataagaaa agtcagatga gtgagagtgg
     1201 atttgcgtca atgtttagtg acgtaaattc acgataagcg aatattttgc atgaagcaaa
     1261 ataaagcgta aaaggagttg agagcgtgac acgctttcgg ctcctttttt cgttgtcact
     1321 tgtgacagcg aaaaccctcg gagagcgttt ggaattgtga cagccatgtc acaattagga
     1381 aaatgttact ctttgacaaa gagtaacaga aaggtttaca ctctttttat tagcgacagc
     1441 cgaaggaggg ttttaacttg gggttcagta tttcgttcaa aaagggaaca aagaatacaa
     1501 gcatttctca taataatcgt gagttaactg atagccaaaa aatcaatggt ttgtctggta
     1561 gaaaataatt agcagattta caagtgggaa ataaaaagct gaaagaagaa aattgcattt
     1621 caaactacat tttctacaat agctctaaaa cggcatattt tgcgttctga gcggttttag
     1681 cgtttatttc gtttagttat cggcacaatc gttaaaacac gttagaggag cgagaatgga
     1741 gcctgtaagg cgactggtag cggaacggtc gtgttagatt gtgaaagccg atagctgaat
     1801 gaaataaaca gcgggtgcgc ccttccattt cggcaggagg aggctcaagg ggtttgggga
     1861 atgacattcc ccaaggtctt gtgtctttta tactcgggag tatttgtcag tttttgtttt
     1921 ctggtgttat aattatcaaa aatcgaaaaa agagc
//
LOCUS       DACEXQ010000310         1947 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.317, whole genome shotgun sequence.
ACCESSION   DACEXQ010000310 DACEXQ010000000
VERSION     DACEXQ010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.317"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            15..284
                     /locus_tag="IXH04_002725"
     CDS             15..284
                     /locus_tag="IXH04_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853263.1"
                     /translation="MKIDYLELINEIAKYKTGEEIEILRDVYDQLEEAGIEGINNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLSELEQV"
     gene            446..748
                     /locus_tag="IXH04_002726"
     CDS             446..748
                     /locus_tag="IXH04_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853264.1"
                     /translation="MDKKAAMKRIIELTHSENWQEDKEIVTEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEKK"
     gene            745..1056
                     /locus_tag="IXH04_002727"
     CDS             745..1056
                     /locus_tag="IXH04_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853265.1"
                     /translation="MNELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGTNVQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDS
                     K"
     gene            1053..1271
                     /locus_tag="IXH04_002728"
     CDS             1053..1271
                     /locus_tag="IXH04_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr domain protein"
                     /protein_id="HAQ1853266.1"
                     /translation="MIGLTIFWCAIGGLLAFLGYYLICESRKGRKYTVTGIVLLIIGI
                     LVAVGAELNLYVNGTQEDLVRFMFWMKN"
     gene            1282..1825
                     /locus_tag="IXH04_002729"
                     /pseudo
     CDS             1282..1825
                     /locus_tag="IXH04_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          727 a          288 c          462 g          470 t
ORIGIN      
        1 taagaggtgg aaaattgaaa atcgattatc tagaactaat taatgaaata gcaaagtata
       61 aaactggtga ggaaatagaa atcctgagag acgtatatga tcaactcgaa gaagctggaa
      121 tcgaaggaat taataatgat cgttcgagtt ggagtaaact cagatactat ttcgcactct
      181 atatcgatac aacacaatta agaaatttag cttatacaaa attactattt gttgattgcg
      241 ttaaaggatt gcaaaaacat cttagtgaac ttgagcaggt gtaatcagat ggatctaaaa
      301 acatttacag cacagatcga actaatgcat caagaagctt taagacaaag cgcctcgtac
      361 gaagacaagt ggctcaacac gttccatggt ggacgtgaga gcgcacttga tcaagtactc
      421 aaattattga aaggggaacg tcgggatgga taagaaagcg gcaatgaaac gaatcatcga
      481 actgacacat tctgagaatt ggcaagaaga caaagaaata gttacagaag tccaaaagct
      541 cggtaaatca atgtggactg aaaagcccaa acggagaacg ccgagaaaga ttgcaatttg
      601 gcatggtgat cgaattctag taacaggtac tgctgaacag ttatctgaaa ttactggtct
      661 gagcaaaaac atcatctggg atagagcaag gagcttatgg attgattcaa aaggacgaca
      721 atttaggtat gtggaggaga aaaaatgaac gaactaatca caaaagtaga gcagtgggct
      781 aaagacaagg gactggatca agcagatcca aaagcacagt ttttgaaagt agctgaggaa
      841 ttcggggaaa tcgcttcagc aatggcaaga agtaatgatg agctatttaa agatagcgta
      901 ggagacgtaa tcgtcacgtt gattatcctt tccatgcaaa aagggacaaa cgtacaagag
      961 tgtttagaaa tggcatacaa cgaaatcaaa gggcgcacag gaaaaatggt agatggtgta
     1021 ttcgtgaagt cgagtgattt ggaggacagc aaatgatagg tttaacaata ttttggtgtg
     1081 cgattggtgg tttactagca tttctaggat actacctcat ctgtgaaagt agaaaaggaa
     1141 gaaaatatac agtgacaggt attgtcctgt taattatcgg aattctagtt gcagttggag
     1201 cagaactgaa tctttatgta aatggtacac aagaagattt ggtgcgattt atgttttgga
     1261 tgaagaatta ggaggaaaaa aatgaacgaa aacaaattaa tcaaattagg tgtagcagga
     1321 gcagtaatcg taggtattgg agttatcgga ggatttaagt tcttcgaaaa aatcgataat
     1381 ggatatgtgg gtgtgcgcta ttcaatgaac ggcggtatca aagatgaagc actgacgcaa
     1441 ggtgtgaaat ttgtagggat tgacaaagtg atccaatatc caattcgctt gcaaactatc
     1501 caatcaaaaa atatttgtcg aagacgtaac acttggtgtt ccagatgtcg ataaagaaac
     1561 acaaaaatca atcgatgcga tcattcgagc tggtcaagaa aatgaaaaag cgaagctaga
     1621 tgcagaaact gcaaaaactc aagctgatag tgaagcttac aagaaaacaa aagctgcaga
     1681 agcagaggca gaatctaatc gcaaagtcgc cgaatcagta acagacaatt tgattcgtta
     1741 tgaagaagct caagctcgca aaaagcatgg atgggtaaca gtaaatggag cagatactgt
     1801 agttacggat gaagcaggca aataatatgg gattctttat ggctaaaatt ctcttgttct
     1861 taggtttagt aggagcagca tatctcgtgt atgcgctcct ttcccaaact gatgacaaag
     1921 aagatgacaa caacgatgaa atgaaat
//
LOCUS       DACEXQ010000311         1942 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.318, whole genome shotgun sequence.
ACCESSION   DACEXQ010000311 DACEXQ010000000
VERSION     DACEXQ010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.318"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..153
                     /locus_tag="IXH04_002730"
     CDS             <1..153
                     /locus_tag="IXH04_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAQ1853267.1"
                     /translation="NKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFSFSVSTEIKV
                     LMGITA"
     gene            159..575
                     /locus_tag="IXH04_002731"
     CDS             159..575
                     /locus_tag="IXH04_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853268.1"
                     /translation="MEFREGAFDFQTSQQNRKFIPEDFQNLQLENMRLEDLFRLADDD
                     KYRFEVLKIKKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNR
                     NSTDVVMITDIYVEDEVIIKYMKPVIKLVNKNKGNG"
     gene            681..944
                     /locus_tag="IXH04_002732"
     CDS             681..944
                     /locus_tag="IXH04_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ1853269.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDLKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            944..1216
                     /locus_tag="IXH04_002733"
     CDS             944..1216
                     /locus_tag="IXH04_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ1853270.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTYEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            1293..1622
                     /locus_tag="IXH04_002734"
     CDS             1293..1622
                     /locus_tag="IXH04_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1853271.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            1612..1899
                     /locus_tag="IXH04_002735"
     CDS             1612..1899
                     /locus_tag="IXH04_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ1853272.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
BASE COUNT          734 a          238 c          386 g          584 t
ORIGIN      
        1 aataaatttt atcaaagtct ccgtacagcc tcttccacga ttaagggcat ggagaccatt
       61 cgaggaatat ataaaaagaa ccgaagaaat ggaacgctct tcagcttttc ggtgtctact
      121 gaaatcaagg tattaatggg aataacagcc taagatattt ggagttcaga gagggcgcgt
      181 ttgattttca aacttcgcaa cagaaccgaa aatttatacc ggaagatttt caaaacttac
      241 agttagaaaa catgcgttta gaagatttat ttagattagc agatgatgat aagtatagat
      301 ttgaagtttt gaaaattaaa aagaatgaac tattgaataa gttatatcct gagaatgcga
      361 taaaagcaca ttttggtgat gctaatcata tgtatagttg gttcattcct aaaagattta
      421 gagggaaaat caatgctgga gatcaagtga aagtactcaa tcgaaatagt acagatgtcg
      481 taatgataac agatatctac gtagaagatg aagttataat caaatatatg aagcctgtga
      541 taaaacttgt taataaaaat aaaggaaacg gataacactt tacaatgctg ttttggtact
      601 ccaatttaaa caaaacaatc gaatttacgt acttaattaa gtacgttaga atggagatat
      661 aaattagagg gggagtagat atggcaaaaa cagtcttaaa tcctagctca gcacggaaaa
      721 atttttataa gttgttgaaa gatgtaaatg agaatcatac ggaaattgaa atcattagtg
      781 agcgcagtga aaataatgcg gtgcttattg gtttggatga tttgaaagca atcaaagaaa
      841 cgctattact tgagcaaata ggcactttgg gtgttgttcg taagcgagaa aaagatgata
      901 gtggatttac taatgttgat gaaattgatt gggataactt ataatgaata actattctgt
      961 gatgattaaa aactcggcta aagctgattt aaagaaaatc aaacagtcta atttgaaacc
     1021 tcaatttgag aaggttattc agactttaaa ggaagatccc tatctcccta cccaatcatt
     1081 tgagaagtta aaacctacat atgaaggtag gtattctaga agattgaata gacaacaccg
     1141 ggttgtttat aaggtagatg aagatgaaca cgtggttgaa atttattcag caaggacgca
     1201 ttacgagtca cagtaattac ttgtaaatac attatctcgt ggtataatgt atttactaag
     1261 taattacata ctgttagaaa tgagggattt aaatgattac aaaaacaaga aaacaaggca
     1321 attcaatcat gttgacagta cctaaagatt ttaatgttcc taatggagtc gaagttgaag
     1381 caaagttggt agagaatggt attctttatg aatttgttga accacaaaaa gaattttatg
     1441 attttagcga agatattttg tctgatataa ttgcagaagg ctacgataaa gatgagattc
     1501 ttgtagaatt taagaataga aaaaacaaaa tgcattcttc ctttagagat attgctgaag
     1561 atacacttac taattctaaa gtaatgacta aagaggagtt ggcaacagaa attggcttat
     1621 gaaatcgttc cttcaaaaca tgtcattaag tatttaaaaa agctaaaaga gaagccattg
     1681 aaagaaaaat ttttgaattt aatttatgat gaaattgcga ttgatccata taagggtgaa
     1741 caaaaaactg gggatttatc aggtatttgg tctagtgggt tcaaatatgc gggcacaaca
     1801 tatagagtag cttacgagat tatagaaaac aaggttattc cagtattgtt atgtggaacg
     1861 catgaaaact tttatgaaca gttgaaaaaa ttaatctagt gatttgttgt cgttcttaag
     1921 aaggacggct tcttttttat cg
//
LOCUS       DACEXQ010000312         1928 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.319, whole genome shotgun sequence.
ACCESSION   DACEXQ010000312 DACEXQ010000000
VERSION     DACEXQ010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.319"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..367
                     /locus_tag="IXH04_002736"
     CDS             <1..367
                     /locus_tag="IXH04_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAQ1853273.1"
                     /translation="KKQIVPKEIETWWRIEMQLRRGKATDWHAMVRESLDSFASPHYL
                     PGDVKPVDRIMIKGLNQDHSEWAYISRNLKYRLRKLLKEESQNDELTNHLRETFKESA
                     NDLKIELDTWLLGLDVTEK"
     gene            567..770
                     /locus_tag="IXH04_002737"
     CDS             567..770
                     /locus_tag="IXH04_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853274.1"
                     /translation="MSQLGKCYSLSKSNRKVYTLFISDSAKEGFNLGFSISFKKGTKN
                     TSISHNNRELTDSQKINSLSGRK"
     gene            1330..>1928
                     /locus_tag="IXH04_002738"
     CDS             1330..>1928
                     /locus_tag="IXH04_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAQ1853275.1"
                     /translation="MSKKGIIVNEWEKLNRISNRRLSVDDKVHPLKLSWSIDRITIVG
                     KLNDTIAYWTQNGVVFVDFEQLMRLNENQGYLKSVGTNGWQLFDQYGEDIAYIEMLKF
                     HPDKGRIDFNPNKIGEFLKGSMKQFIHDLFLDAHFSRADIACDILGVPDDFITQYRIV
                     DPVSFKPIYGRSGKLETAYWGSRASERQVRMYNKKLEQER"
BASE COUNT          627 a          290 c          453 g          558 t
ORIGIN      
        1 aaaaaaacag attgtaccaa aagaaatcga aacatggtgg cgtatagaaa tgcagctaag
       61 acgtggcaaa gcaacagatt ggcatgcgat ggttcgggaa agtttagata gctttgcaag
      121 tcctcattac ttgcctggag atgtaaaacc tgttgataga attatgataa aagggctgaa
      181 tcaagatcat tcagagtggg cgtatattag cagaaatttg aaatatagat tgcgcaaatt
      241 gttgaaagaa gaaagccaaa acgatgagtt gacgaatcat ttacgagaga cgtttaagga
      301 atctgcaaac gacttgaaaa tagaattaga cacgtggctt ttgggtttgg acgtgacaga
      361 gaagtgatac cgaataagaa aagtcagatg agtgagagta aatttacgtc aatgtttaat
      421 gacgtgagtt tacgataagc gattattttg cgtgaagcaa aataaagcgt aaaaggagtt
      481 gagagcgtga cacgctttcg gctccttttt tcgttgtcac ttgtgacagc gaataccctc
      541 ggagagcatt tggaattgtg acagccatgt cacaattagg aaaatgttac tctttgtcaa
      601 agagtaacag aaaggtttac actcttttta ttagcgacag cgcgaaggag gggtttaact
      661 tggggtttag tatttcgttc aaaaagggaa caaaaaatac aagcatttct cataataatc
      721 gtgagttaac tgatagccaa aaaatcaata gtttgtctgg tagaaaataa ttagcagatt
      781 tacaagtggg aaataaaaag ctgaaagaag aaaattgcat ttcaaactat attttctaca
      841 atagctctaa aatggcatat tttgcgttct gagcggtttt agcgtttatt tcgtttagtt
      901 atcggcacaa tcggtgaaac acgttagagg agcgaaaatg gagcctgtaa ggcgactggt
      961 agcggaacgg tcgtgttaga ttgtgtagcc gatagctgaa cgaaataaat gctggcagcg
     1021 ccattctatt tcggcaggag gaggctcaag gggttcgggg aatgaaattc cctgaggtct
     1081 ttttattttt tctagaggag tgctataatc aaagaaaatg agaaaaaaga gtaaaaaaat
     1141 agtcgagcag tgttgacgca ctctcgacct ttacggaaac tacttcacct agttccgtaa
     1201 ctcaagttca ttatacgaat atttttaggt ttcttcaagc tagagatttt tggatgtgga
     1261 tttttagagt tacgtgaact ctggaaattc tttccaagaa tctctttttt cgttgaggag
     1321 gagctatttt tgagtaaaaa aggcataatc gtaaatgagt gggaaaaact taaccgtatt
     1381 tctaacaggc ggttaagtgt tgacgataag gtacaccccc taaaattaag ttggtcaatc
     1441 gatcgaatta caatcgtcgg aaaactgaat gataccatag cttattggac gcaaaatgga
     1501 gttgtgtttg tcgattttga gcaattaatg cgtttgaatg aaaatcaagg gtatttgaag
     1561 tcggttggta caaatggttg gcaattattt gaccaatatg gagaagatat cgcctatatc
     1621 gaaatgttaa aatttcaccc tgacaaaggt cgtattgatt ttaatccaaa taaaattggc
     1681 gagtttttaa aggggtcgat gaaacaattc attcatgatt tgtttctgga tgcgcatttc
     1741 tcacgtgcag atattgcttg cgatattctc ggtgttcctg atgattttat tacccagtat
     1801 cgaatcgttg accctgtgtc ttttaagcca atttatggcc gttctggcaa attagaaaca
     1861 gcgtactggg gtagtcgtgc cagcgagcga caagtgcgga tgtacaacaa aaagcttgaa
     1921 caggaacg
//
LOCUS       DACEXQ010000313         1921 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.320, whole genome shotgun sequence.
ACCESSION   DACEXQ010000313 DACEXQ010000000
VERSION     DACEXQ010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.320"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..473)
                     /locus_tag="IXH04_002739"
     CDS             complement(<1..473)
                     /locus_tag="IXH04_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1853276.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKI"
     gene            742..1638
                     /locus_tag="IXH04_002740"
     CDS             742..1638
                     /locus_tag="IXH04_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAQ1853277.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1788..>1921)
                     /locus_tag="IXH04_002741"
     CDS             complement(1788..>1921)
                     /locus_tag="IXH04_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ1853278.1"
                     /translation="RLTEIDYINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
BASE COUNT          656 a          350 c          350 g          565 t
ORIGIN      
        1 ctgattttag caaaagccag ccaatattct gtttctgcga ataaccgtac cgaataaaga
       61 ttgatgatcg tagttaaaac agccaacata aaaacaaata cccaggctgg aatgtgcggg
      121 aaccacaact gcaaaaagga agcagctgca acagcttcag cgatgatatt gataatccac
      181 atagaccagt agacccaatc agtaaaatca gcagaatgag cacctagata agggcgtatc
      241 aaaccagata atccgtgatg cgtgtcgcca ccgcttaaaa cgatttttcc cagacctttc
      301 atcacgatat ataaaatcag cccaccaatc aaaaaagcaa tcaatacaga agggccggcg
      361 atcccaatcg cttcgccact tcctttaaat agacctgcac cgattgctcc acctaaagct
      421 aacatggtga tatgtctgga gctaagttgt tttttcaatt ttttatcttc cataaatgac
      481 ctctttcttt taaattcaca atcaaaattt atattaagaa taaatagcaa tatttaacaa
      541 tgactaatga taacaaattt tcagacaatt acaactattc agaaaacttt taggcgaatt
      601 ttaatagaag aatgatttat ttcatcatat tttcatacaa atgttaaatt ttcatacaaa
      661 tataggaaac gttttactgg ctttttacta tttaaacagg ctatcatagg aaaaagtgac
      721 agagaaagtg aggcggaaaa gatgaacaaa aatgacgtac tgctagttgc aaaaaataaa
      781 tcactttatt ttttgccttc catccgctct ttagatgttt ggtccggaaa agatttacta
      841 cagtcggaat tcattaaatc agctagacaa cagttggcaa attatcagct gatcgttttt
      901 ttagattatg gatttgatat ggagatggct cgacgcgtgc gcccatttac caatgcaaaa
      961 attgtgttat ttttttggaa tcacttcaaa gcagaacacg aggcgatgct gaagcaggca
     1021 caagatgaac cggcaatcga tgagatttat cattttgatt ttttggaagc aaaagaactt
     1081 gggctgaaac ataatagttc attttattcg aaacatatga atttttcttc tagcgaacca
     1141 aagacggatc tattttttgg tgcaacgaat aatgggagaa aagaacgagc agaaagctat
     1201 aagcaggaat ttataaaaag agatgttacg accaactatt tcattcttcc acatcgaggg
     1261 aatgaacaac ctggatatct tacttatgaa gagtacctga aaaaaacagg ggaatcgcgc
     1321 gggattctgg aactgttgcg ggagggtcag caaggagtga ctttgcggac atttgaatcc
     1381 atgtattttc aaaagaaact agtgacggat aatcaagcga ttgctcatta tcatttttat
     1441 cgtcctgaaa atattttttt gcttcgagaa cgtgatttag atgaattacc ggaatttctg
     1501 catacaccat ttaagccaat cctatcagag ctattggaat tttatgatgc agaaaattgg
     1561 gcaaaacgat ttatcactaa cgattccact atttatgagc aatacgaata tgacctctcc
     1621 tctgttgaaa acaaataaaa aacctacatt ttgaagagat tatgtactga aacaaacaag
     1681 cacaattctt atggcatcat gagagttgtg ttttcttttt gatcttttat tgggcttaaa
     1741 aaaagagaac cagctgctga ttcagctgat tctcatcaga gagcctttta ttctgcgcca
     1801 aggattcctt cttttgcatg gactaattgg gtcaaaaagg cacaatatgg tgtagcaata
     1861 tcatattttt gtccttttcg ccaaatggca ccattgatgt agtcgatttc agtcaaacga
     1921 t
//
LOCUS       DACEXQ010000314         1911 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.321, whole genome shotgun sequence.
ACCESSION   DACEXQ010000314 DACEXQ010000000
VERSION     DACEXQ010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.321"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            256..1770
                     /locus_tag="IXH04_002742"
     CDS             256..1770
                     /locus_tag="IXH04_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAQ1853279.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
BASE COUNT          676 a          310 c          458 g          467 t
ORIGIN      
        1 tccagacctg atccactagc ataataacga cgatgatacg taataaaata tagattccgt
       61 ttgtgattct acctgcagtt tttgtactgt tcgtaaaaaa ggttttcaaa tgattcataa
      121 aataactcct tcatgtatat ccttaattat actaagaagc gtgtgtttac caaagctata
      181 tgacttaaag aaaaacagag ttgatattgt gaaaaaaatc acttaatata gtataaggag
      241 aaaaggggga tacaaatgaa aaaattagtt gctggtttat taacagccgt tgcacttctt
      301 gctatgagcg catgcggcgc agatcagaaa aatacagcgc agacaagttc tagtacgaag
      361 gaatctgccg aagtatcatc aggcgcttct gaacaagtat atacagatcc aaaagaaatg
      421 aaagaatctt atgatgtcgt gattattgga gctggtggtg cagggatggc agcagctatc
      481 gaagcaaaag atgctgggtt gaatccagta attttggaaa agatgcctgt tgccggagga
      541 aacacatcta aatcatcaag cggtatgaat gcttcagaaa caaaatttca aaaagaagaa
      601 ggaatcaaag actcgaatga tgccttctat gaagaaacgc taaaaggtgg tggaggaacg
      661 aatgataaag aaatgcttcg atactttgtc gatcactctg ctgatgcggt cgattggcta
      721 gatcaaaacg gtatcaaact ggacaactta acgatcactg gagggatgag tgaaaagagg
      781 acacatcgtc cgtctgatgg ctcggctatt ggtggatact tagtggaggg gttattgaat
      841 aacgtccatg agcgggaaat ccctattttt gtcaacgcag atgtgacaga cattaagaaa
      901 aatggggaaa cggtcaatga agtgactgta catgttcaag gagaagaacc aaaagaggtc
      961 accggaaaag caatcgtagt taccacaggc ggttttggtg ccagcaaaga atttattgaa
     1021 aaatatcgtc ccgatttagc tgattatatt acgacaaatc aagaaggatc aactggagat
     1081 ggtatcacca tgattgaaaa attaggtgga caaaccgttg atatggataa aatacaaatt
     1141 catccaactg tgcagcagga tgagggggtc ttgattggtg aggttgttcg cggagaagga
     1201 gcaatcttgg tcgatcaaga ggggaaaaga tttgtcaatg aaatggatac aagggataaa
     1261 gtatctgcag caattacagc attgcctgaa aaatcagctt atttgatttt tgaccaaggc
     1321 gtgcgtgatc gtgccacagc tattgaattt tatgaccaaa aaggatatgt cacaactgga
     1381 aagacaatgg aagaattagc agaaaaaatc gatttaccaa aagaaacact agcaaaaaca
     1441 gttgatggat ggaatacgga tgttgcggag aaaaaagatg ctcaattcaa ccgtgccaca
     1501 gccatggaac atgacttatc gtctccgaat tattatgcga tcaaaatcgc accaggaatc
     1561 cattacacga tgggcggtgt gaaaatcaat acgaacactg aagtattaga taaagaaaac
     1621 cagccaatta gggggttata tgctgctggt gaagtcacag gtggcttaca tggaaacaac
     1681 cggatcggtg gaaattcagt tgcggagatc attatttttg ggcgacaagc gggcatacaa
     1741 gcagctcgat ttgcttcgga aaatgaatga aatagaacgt aaataaaaag aggttgtgaa
     1801 atcagcgttt tgagctaata ccgagggtca gctgattgaa cactgtttag tgaagaggtt
     1861 gtgaaaaagc tgccctgcat caagtaataa gaacagccaa gtaaaaacag c
//
LOCUS       DACEXQ010000315         1799 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.324, whole genome shotgun sequence.
ACCESSION   DACEXQ010000315 DACEXQ010000000
VERSION     DACEXQ010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.324"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..635)
                     /locus_tag="IXH04_002743"
     CDS             complement(<1..635)
                     /locus_tag="IXH04_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAQ1853280.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGS"
     gene            799..1647
                     /locus_tag="IXH04_002744"
     CDS             799..1647
                     /locus_tag="IXH04_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1853281.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
BASE COUNT          571 a          354 c          289 g          585 t
ORIGIN      
        1 gaaccatgat tccaaacacg tgcgacttgt tccaagtcaa aagcatactc acttttttgt
       61 acaagttgga atccttcacc gattgcttgc atcattccat attcgattcc attatggatc
      121 attttcaaga agtgtccgct tccggatgca ccagcataca gatatcctct ttcaattgct
      181 acatctttga ataattgttc cacggataga aaagtttctt tatctcctcc aatcattaag
      241 caggcttgat gtcgtgctcc ttcgattcct ccagaagtcc cacaatccaa gtaaccgatt
      301 tcatttttct gaaactcttc tgctcgacgg attgaatctt tataataaga gtttccgcct
      361 tcaataataa tgtctcctgg tccgagtttt ggaaaaagtt ttcttaacat gttttcggtt
      421 atctctccag caggaagcat taaccagatg atttttttct gctcaaaagt agctagaaat
      481 tcagagatct cttgataagc aggaatgtca ttttctcttg cctgactaac agctgtactt
      541 gaaatgtcga gtccttgtac tttgtgatgg tgctctttta agtttaaagc taaattcaat
      601 cccatttttc ccaaaccaat tacaccaatt tccatgatag cgcctccatt tcttataccc
      661 tcattataca aaaaaatttt tttgtaaata gattttacaa aaatattttt tgtttgattt
      721 tttcctactc tccttgtatc tttaacatta atgagaacgg aaaaaatgat aatcttatta
      781 tgaaaatgag gaattctcat gagcgtctcc ataagactac aaatcaaaac aatgtacaac
      841 gacttcagcc caaaagaaca ggctatcgcc gattatattt tagaaaatcc aagcaaagta
      901 agtcatagtc ctattagcaa tttagcaagt gaactgggca ttgctgactc tacttttttt
      961 caatttacaa aaaaacttgg ttataatgga tttaaagatt ttaaaatggc catgttgatc
     1021 caagaaaatg atctatctgc tatttctatc cacgaaaata ttcagaaaac agatactgag
     1081 ctcactatgg cgcaaaaggt gttcgagtct agtatttcaa ctttaaatga cacaaaaaag
     1141 ttgttacaac aaaaggatct aaaaaaagcg gctactatta tcaatgaatc taaacgtgtc
     1201 tattttttcg gtgttggcgg ttcagagatt gtagcaactg acgcttatca taaatttttg
     1261 cgttccccta tcgcaacttc tcacagcacg gattaccata ttcaattgat ggaagcttct
     1321 ttattgacgg aaaaagactg tgctgttttg atttctcaca cagggcaatc aaaagaaaca
     1381 atccatattg ctgaaaccgt aagaaaaact ggagcaaaaa caatcgttat tactagccaa
     1441 gctaattctt cccttgctaa acttggcgat gtcgttttta tttctatttc tgaagaaaca
     1501 gaattccgat ctgaagcttt agcttcccgt atttctcaac taagcatttt ggactctcta
     1561 tatgtgattt tgatgtttta caacgaaaaa gaatcacgtc gttctatcgc taaagttcga
     1621 catactattt ctgaaatcaa agaatagcac cataacagaa aaagcgaaaa atccataaaa
     1681 caaaaaaagc cactaccaaa tatttggtag tggctttttt ggttctgcaa gggtggtaca
     1741 gaacatcaat agtcggtggg ttagaacgta tgtcatcgca tctttggaga aggttgtat
//
LOCUS       DACEXQ010000316         1785 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.326, whole genome shotgun sequence.
ACCESSION   DACEXQ010000316 DACEXQ010000000
VERSION     DACEXQ010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.326"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..374)
                     /locus_tag="IXH04_002745"
     CDS             complement(<1..374)
                     /locus_tag="IXH04_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ1853282.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKR"
     gene            complement(635..>1785)
                     /locus_tag="IXH04_002746"
     CDS             complement(635..>1785)
                     /locus_tag="IXH04_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAQ1853283.1"
                     /translation="GRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQE
                     AKAGDLIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKF
                     VTARKIEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVS
                     RPEVIEKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTF
                     LTPARGLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTY
                     SIMSIEERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVI
                     KKPKQLTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
BASE COUNT          487 a          380 c          315 g          603 t
ORIGIN      
        1 cgcttcattg cttggataaa atccggcatg ataaaacgtt caggagcgat ataaagtaaa
       61 cggtactgct gtctatcgat ttcttggaag cgtctagctg tttcgtaacc atctaacgag
      121 ctattgataa aagtggcggg tatacctaat tgattcgccg cgtccacctg atccttcatc
      181 aaagagatta aaggggacac aaccacagta agcccatcta atagtaaagc tggaagctgg
      241 taacagattg atttcccact tccggtaggc atgattccta atacattttc ctgatcaatg
      301 actttctgga taatttcttt ttgaccaggt ctaaactcat catagccaaa gtattgtttt
      361 aataagtcgt tcatcttccc gctcctttct cttgccattt tacttcgatt ttcttcgaag
      421 gacaagttga tctcctttcg tgaaacatta tcttattatg aaacaaaaac tctctagaaa
      481 taagtttttt aaaagtcttt taaacgcctt tcggcgtatt ttctgaattt atgtccattg
      541 acaaagatga cttctccaca gaaaagagtt ggacaaaaaa agaaaccagc tattttttat
      601 aacaagctgg tttctctctt catcctttta cagattattt tttcttttta gatgcttttt
      661 cacgagcatt tttctctaaa atttgttttc tcaagcgaat gctttctggt gttacttcgc
      721 aatattcatc atcattcaag aattccaaag attcttccaa cgtcaattgt tttggttttt
      781 tgatgacaga tgtttggtct ttagtagcag accgtacgtt tgtcatttgt tttgctttcg
      841 tgatgttgac agtcaaatca ttatcacggc tgttttcccc gataatcatt ccttcatata
      901 cttctgtacc tggttctacg aagactgtac cgcgttcttc gatactcatg atagaataag
      961 tcgttgcttt tcctgtatcg atcgaaacaa gcgccccttg gtgacgtccg ccaatttgac
     1021 caggaagcat tggcagatat tggtcaaaag tatggttcat gattccgtaa ccacgtgtca
     1081 ttgaaaggaa ttcagttgaa taaccaatca aaccgcgggc aggtgttaag aatgtcaaac
     1141 ggatttgtcc gtttcctgta tggaccatat cttgcatttc acctttacgt aagctcaagg
     1201 actcgataac actacccata tattcttctg gtgtatcgat ttgcacgcgt tcaaacggtt
     1261 cgcattttac accgtcgatt tctttttcaa taacttctgg acgagatact tgcaattcgt
     1321 atccctcacg gcgcatgttt tcaatcaaga tagacaaatg caattctcca cgaccagaaa
     1381 ctgtccatgc atctggagag tttgttggtt caactcgtaa ggatacatct gtttgaagtt
     1441 ctgccatcaa acgttcttca atcttacgag cggttacgaa tttaccttct cgaccggcaa
     1501 atggtgaatt attaactaag aaagtcattt gcaatgttgg ttcgtcaatg tgaaggatag
     1561 gtaacgcatc ttgatgatcc acaggagtaa ctgtttcacc gacaaagata tcttccatac
     1621 ctgagacagc aatcaagtct ccagcttttg cttcttgaat ctctagacgt tttagtccaa
     1681 agaaaccaaa taatttcgtt acacggaatt tcttaactgt tccgtctaat ttaattaatg
     1741 ctacttggtc tcctactttg attgttcctc taaatacacg tccga
//
LOCUS       DACEXQ010000317         1771 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.327, whole genome shotgun sequence.
ACCESSION   DACEXQ010000317 DACEXQ010000000
VERSION     DACEXQ010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.327"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            226..405
                     /locus_tag="IXH04_002747"
     CDS             226..405
                     /locus_tag="IXH04_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853284.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDVPALKK"
     gene            464..820
                     /locus_tag="IXH04_002748"
     CDS             464..820
                     /locus_tag="IXH04_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853285.1"
                     /translation="MLKTKKNSYKLKLSILSIICLLGMIISGHLYRVAYTQFIEYKVH
                     SLGLAGDRYVEYVHYSLKCSSYILLWLIFSLFELLNLIAISSYHLDKLNTKSSRYNEV
                     FKENVKLESDLNKLKK"
BASE COUNT          611 a          227 c          335 g          598 t
ORIGIN      
        1 gtgatccatt tgaacaagat tattccctta ttgttagtac tgtttgttgg cgaaatactt
       61 atgcgagata gtgcagctgt acagttaaac ctcgctgtat ttttggtaat actggtgttt
      121 tgtttgatct tttggcggtt tgtattagtc ctcgtgagtc tactattcaa gatgattgca
      181 gccatatttt tcatagggtt atttcgatga aaggagtgag taccaatgcc aattgcaata
      241 ggaaataaac ggttacccgt gacattagat gaaaagagac aaaaagaatt gcagcaacta
      301 aagcagaagt atggcaaaag tgaatctaag attatgtgta ttgcgttaga tctattgatc
      361 gcgcaagaaa aagcaggatt tgatgtacca gcgctaaaaa agtgacgtca ctttttaatt
      421 tgatctatat aagtgatatc acttttacca aggagggaat taaatgttaa aaactaaaaa
      481 gaattcgtac aaattaaagc ttagtatact atctattatt tgtcttttag gcatgataat
      541 tagtggtcat ttgtatagag tggcatatac gcagtttatt gagtacaagg ttcactcttt
      601 aggattagct ggggatcgat acgttgaata tgtccattat tcattaaaat gttcttcata
      661 tattcttctt tggcttattt tttcattgtt tgaattgcta aatttaatag caatatcttc
      721 atatcactta gataagctaa ataccaaatc tagccgttat aacgaagtgt ttaaggaaaa
      781 tgtgaagtta gaaagtgact taaacaagct aaaaaaataa aaagaagaaa acataagtta
      841 actaaaatta ttttattaag aatgatggaa tataaggata aggttaccaa cataacgatc
      901 attacttctc tgatatgcta tggacttcta tcaatcagta ttttgtctat agattatgga
      961 gtaactatac tgcaagcctt agttttggct atgcctatgt atctatcttt gtttatcatg
     1021 catgaaatat ataagctatt tgttttatca aaaaatgtag aaatacgggt aaatattttg
     1081 gtgaatagtt atcaaaaaga actcaaagaa agaagacggt ggcggtattt attgaaagga
     1141 tgaaaaagta ggatttgaca tactggtatt cagaaagtgc cgtcactttt aaaacaatgg
     1201 aaaaggtgga gataagatga agaaaaaaca aactaaacta tcattagcag atatttaaca
     1261 cagttaaccg caactgaaga tggtgttgtt gaatttgaac aatctgaaat tgcggtagta
     1321 gatgatcgtt accttaaaat gccttacttc tttgaccaag caaaggtcat ttgcctttgt
     1381 cactacgatg gggtacgtga ttttaggcgt taaaaagtag tgattatatc aactttacga
     1441 gtaaatataa atttagacat gaaactgaat tatccaatgg aaaatcatgg tttataatct
     1501 agaaaaatta tagtaatgat tggattatat atattctttc gtattttcgt acaactgtag
     1561 cttgtcaaaa ttgagaaatt taaccgatta ggtactttac atcgttattt gggtaatttc
     1621 taaaacatga agcttaccaa cgaataaact gagactatct tattaattga tcaatataag
     1681 aaaatatctt tgaggagatg atgtactatg ttttcgtcta tccacgattg gttaggtggt
     1741 cttttcggcg gtggaggtaa aggtttttaa t
//
LOCUS       DACEXQ010000318         1753 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.328, whole genome shotgun sequence.
ACCESSION   DACEXQ010000318 DACEXQ010000000
VERSION     DACEXQ010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.328"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..933)
                     /locus_tag="IXH04_002749"
     CDS             complement(<1..933)
                     /locus_tag="IXH04_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1853286.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPF"
     gene            1080..>1753
                     /locus_tag="IXH04_002750"
     CDS             1080..>1753
                     /locus_tag="IXH04_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ1853287.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDG"
BASE COUNT          537 a          350 c          344 g          522 t
ORIGIN      
        1 aaacggaaac cgttgaagga attcttttac atcaacgatc cgagccataa aatatggttc
       61 gatctgctct ttgatctgac tatcttcaat caaaaatgct aaaggctcat ttttataaat
      121 atctccatgt acccagtaga tcatggaaaa atgtgcggaa ataaagttcc ataagccatt
      181 tcgagcttct tgatcaagat agaacatttc ttttacgtga aatatttctt cagctaccca
      241 gtagaacaga acacctcttg gttcatggtt gctatcgtag tagacagcgg ctgtgcgctc
      301 atcttcattt tcgaaacgcc aatactcttc ccaatgaaaa tcactgcgga ataaagcacc
      361 atggttttgt tgggcaaatt tgtcataaac ggaaaatacg tctggatgat caactggtaa
      421 tcgctcaacc ataccactta ctgggatttg tttaggtagc tgtgtatctc tgattttaaa
      481 ggaaagttta tcagacatga tttcccatcc tttacgccga tagtagggga tactgtaagg
      541 atacaaataa gagatccatt gcttattctt gcgcatatta tccaatgcaa cgataattag
      601 atcttgcatc aatccatggt tagcatattc tggataagtt ccaactccag tcacaccacc
      661 cattttataa cgtgcaccgt gaatattgac ttcacaagga tagatagcga tttgagagat
      721 cagtttattt tcgtggaacc atccgaacac tttcgatact tccaaaatcg gttttttgga
      781 acggataaaa gctcgtttat tctcaaaccc gctttcctcg atgtctgctt cagtcacttg
      841 gaacacatag gaaagcaatt cattgaattg atcgatatgt tcttgagtga ccggttttaa
      901 ttcaagattt tctctaaaat ctttttgatc cattttttca tctccattta ttagcttgat
      961 aacagtatag caaaattcta caaaaaaaac ttctatagct gactaattaa aaactcttta
     1021 aattactgct tttcgttggt ataatggaaa gtgctgatta tatgaaaaga ggagacgcta
     1081 tggatataaa gaaaatgaaa gaacgccaag agcagattcg taatttttcg atcatcgctc
     1141 atatcgacca tgggaaatct acattggccg atcgtatttt agaaatgacg cacactgtga
     1201 cgtctcgcga aatgcaagat cagctgttag actcgatgga tttggaacgt gaacgtggaa
     1261 tcacgattaa attaaatgct gtagaactca attacacagc gaaaaatgga gaaacatata
     1321 tctttcactt gattgacacc ccagggcacg tcgacttcac ctatgaagtc tctcgaagcc
     1381 ttgctgcctg tgagggtgcc gttttagtgg tcgatgcagc acaaggaatc gaagcacaaa
     1441 cattggccaa tgtttattta gcgttagaca atgatttgga gatcctgcca gtcatcaata
     1501 aaatcgacct acccgcagca gatcccgaac gtgtccgtca agaaatcgaa gacgtaatcg
     1561 gaatcgatgc tagtgaagct gtattagcca gtgccaaatc agggattggg attgaagata
     1621 ttctcgagca gatcgtggaa tatgttcctg caccaagtgg agacttagat gcaccgctaa
     1681 aagctttgat ctttgactct atttatgata gttaccgcgg tgttgttcta aatgtccgga
     1741 tcacggacgg tat
//
LOCUS       DACEXQ010000319         1739 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.329, whole genome shotgun sequence.
ACCESSION   DACEXQ010000319 DACEXQ010000000
VERSION     DACEXQ010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.329"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..818
                     /locus_tag="IXH04_002751"
     CDS             <1..818
                     /locus_tag="IXH04_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1853288.1"
                     /translation="LGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELIA
                     ITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFEK
                     MNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQR
                     AVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLWD
                     KGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1036..1479
                     /locus_tag="IXH04_002752"
     CDS             1036..1479
                     /locus_tag="IXH04_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAQ1853289.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
BASE COUNT          505 a          295 c          365 g          574 t
ORIGIN      
        1 tgttgggcac aggcgttttc cctacccttc agcaattgac tgaccatggt gggtttactc
       61 ctcatggtat gaagggaatc atcagttctt tgcttgtagt tatctattct tacggcggtt
      121 ctgaactgat tgctatcacg gtaagcgaag cagatgatcc taaacgtgcg attcctaaag
      181 caatcaaagg tgtcatggga agaatcattt ccttttacat cattcctttg tttttgcttt
      241 tgattctttt tccttggaat actttagcag gcacgaatgt cagtcctttt gtgatggtat
      301 ttgaaaaaat gaatatccca tttgcagcgg atatcgtcaa ttttgtgatt gtccttgctt
      361 tgttctcatc tatcaattcg ggtgtgtatg catcttcaag aattttgttc ttccgtttga
      421 aggatcgaaa aggttcctct agaaaactag ctgttttaaa caagcatcaa gtaccgcaac
      481 gtgctgtttt attttgtacc agtgttcttt atcttggtgt agctctttct tattttgtcg
      541 gtgacaaatt atttggctat ctggcaggtt ccctttcgta tacagtatta ttgatctgga
      601 tcattatcag tgcggcatcc tttgtactgg cactaagaaa aggaacatta tgggataaag
      661 gaatcagctt ttttgcatta gctgtcttag gattgatttt ccttggtatc ctgttcacga
      721 actccattgg ggtaactgta ttgactggcc tgctttacct gttcatttat ttcagttatc
      781 aaaaaaagaa tgatgcgttc gtattgacga atgaatgaaa tcgatagaaa attttatttt
      841 atttatgtgg aatagatgga gctattgaag aaaggagttg tgacaaaagt cttgttgcaa
      901 ctcctttttc cgaataaaca gtctcatgac ctcttggtaa acggtgttca aaaaccctct
      961 gtgcaaaata attgattgtt ctccatgctc atgacatgat agaagaaagg aatgaaaaaa
     1021 aggagtttgt tttaaatgaa aaagtattgg aagtttttgt tgatgatcgg tgtttcaacg
     1081 gtagttatgt ttggtatgat gtacttgaat gtctatgcgc tcgatcatat cttcttcagt
     1141 cgtacacgag tattcatggc gttgatgatg ggtgcagtca tggcagtcat catgctgttg
     1201 ttcatgtgga aaatgtatga aaacaaaaag ataaatgtta tgatcttagc cgctagtctt
     1261 cttgtttttg ccggttcctt gtttatggta cgaagccaaa ctactgtcaa cgacacagct
     1321 tggatgaagg ccatgattcc tcatcattct atcgctatcc tggcaagtaa acgagctgat
     1381 ttaaaagatc ctgaagtaaa aaaattagcg gaagaaatca ttcaggcaca agaagaagag
     1441 atcgacaaaa tgaaaaaaat gatcaaagaa atggaatgac cattatccgt ccaaataaaa
     1501 aaagaggatg ttccagaagt tatcagcttc aagaaataag aagaagctac ttctggaacg
     1561 ccgtgaataa ggggttgtga aaaagctgcc ctgcatcaag taataagaac agccaaacaa
     1621 aaatagcttc tcctattttt cgtttggtta ggcttattac cgcagatgca agcttttgaa
     1681 caccgtttat taagaggttg taaaatcagc gttctgacct gagtattaga gagacaaat
//
LOCUS       DACEXQ010000320         1728 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.330, whole genome shotgun sequence.
ACCESSION   DACEXQ010000320 DACEXQ010000000
VERSION     DACEXQ010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.330"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..1243
                     /locus_tag="IXH04_002753"
     CDS             <1..1243
                     /locus_tag="IXH04_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAQ1853290.1"
                     /translation="PEDVKELLNFMSRFPERSFRNQLLIEEQFPGATACLGRAALKKE
                     GIYIKKGEQGNKIFVRKTVKGFYEKGRGFVRETEATPEDKKRIESGQIEVIKKPYYTI
                     EKVFDITQTQLKPEDYPKIFPNRVFDFQLDKKGQSELQAGIQAVANNIGIQIRDMTES
                     EVYQRELGQARGAYVRNEFTGEEEIVMNTRNTPTQHLATSIHELAHARMHKFSELDTA
                     TKELQAEMTSYIVCKHFGMDTSEKAIPYIAAWTKNGQTLDDKEAAERGKIMNDVSRVA
                     NEFIQTISNEINQYREREPEIQPAEVKQEHKNTVETAENKNIVPVGSLWIDKKDGKVM
                     EEDTGRTLHLKDAAFIEDPQHNQVILKGKSYGKEIQTALPSEEFALGNPFRFSLETVS
                     EVAKRKNEIYYEQQLSQKERV"
     gene            1304..>1728
                     /locus_tag="IXH04_002754"
     CDS             1304..>1728
                     /locus_tag="IXH04_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853291.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYA"
BASE COUNT          662 a          273 c          369 g          424 t
ORIGIN      
        1 tcctgaagac gtgaaagaac ttttgaattt tatgtctcgt tttccagaac gatcctttag
       61 aaatcaatta ttgattgagg aacaatttcc aggtgcaacg gcttgtttag gtcgtgctgc
      121 gttgaaaaaa gaaggaatct atatcaaaaa aggggaacaa gggaacaaga tctttgtcag
      181 aaaaacggtc aaaggatttt atgagaaagg acgtggcttt gttcgagaaa cagaagctac
      241 accagaagat aaaaaacgaa tcgaaagtgg tcaaatcgaa gtgatcaaaa aaccttacta
      301 cacgattgaa aaagtgtttg atatcacaca aacacagtta aaacctgaag actatccgaa
      361 aatctttccg aaccgtgtct ttgattttca attggataaa aaaggacagt cagaacttca
      421 agctgggatt caagcggtag ccaacaatat tggaatacaa attcgagata tgacagaaag
      481 tgaagtctat caaagagaat taggacaagc acggggggcg tatgttcgta acgaatttac
      541 tggcgaagaa gaaatcgtca tgaatactcg aaatactcct acgcagcatt tagctacaag
      601 tattcatgaa ttagcccatg caagaatgca caaattttca gaattagata ccgcaactaa
      661 agaattgcag gcagaaatga cttcttatat tgtgtgcaaa cattttggaa tggacacttc
      721 ggagaaagct attccttata ttgcagcatg gacgaagaat ggtcagacgt tggatgataa
      781 ggaagcggcg gaacgtggga aaatcatgaa tgatgtcagc cgtgtcgcaa atgaattcat
      841 tcaaaccatt tctaatgaaa tcaatcaata cagagaaaga gaaccagaga tccaaccagc
      901 agaagtgaag caggagcata aaaatactgt agaaacggca gagaataaga atattgtgcc
      961 cgttggttca ctttggatcg acaagaaaga cgggaaagta atggaagaag atacgggtcg
     1021 aacgttacat ttgaaggatg cggcgtttat tgaagatccg cagcataatc aagtgatttt
     1081 aaaaggaaaa agttatggaa aagagattca aacggcgtta ccttcagaag aatttgcgct
     1141 aggaaatcca ttccgatttt cacttgaaac agtgtcagaa gtggccaaac gaaaaaatga
     1201 aatctattat gaacaacaat tgtcacaaaa agaacgcgta taaaacaagt aaaaaagact
     1261 atctgaaaaa cggatagtct tttttctttt gggaggtttg aaaatgaccg aaaaaaaaat
     1321 gagtcttatt gatcgatgta aacagatcga tatagtggat tttgctcgaa acaatggaat
     1381 ggctgtagtc aataaaggac gagactatcg cttagaagac catgattcat ttgtttttga
     1441 tcgaagaaaa caacgttttt attggaacag tcaaaatatt agtggcgaca ttattgaatt
     1501 agctaaactt tttttcatag acaaagagat ccaagactct aagcaacaat ttaaagcagc
     1561 cacagatttt attctaaaga atgaagacaa aacagaacga gtagaaaatc ttcattttga
     1621 gacagaaaag tacaaagatc accccgttga ttatcaacca ctcacagaaa aaggaaggaa
     1681 ttatctgaag gaagaacgaa aactaccaga gtggctgatt gattatgc
//
LOCUS       DACEXQ010000321         1723 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.332, whole genome shotgun sequence.
ACCESSION   DACEXQ010000321 DACEXQ010000000
VERSION     DACEXQ010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.332"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(278..1339)
                     /locus_tag="IXH04_002755"
     CDS             complement(278..1339)
                     /locus_tag="IXH04_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1853292.1"
                     /translation="MNSSDLISVIIPVYNVEKYVGSCLESVCNQTYKNLDIIIVDDGS
                     KDSSNKICEDYALKDSRIRIIHKNNAGLGLARNTGLEHVKGKYVAFIDSDDFVAANYI
                     EAMLNGIKRSNADTCYAGYYEYYDDNNIISKPARYDSTIFTNNAIIEKVLLGMIGTEP
                     SYKVDFILPMSVWHALYSSEIIQQHNIKFPSERDYISEDIIFHISYLQHSKITSFIKD
                     CIYYYRCNNNNSLTHNFRDNEFDCNIELVKKIEGDLSYILENEIFVNKTRRLLLGRCR
                     TCIQKAVRYASFNKEFDLNSYLSKILMNDELIACIHSYPYKQNPIKQRIFNYFLLNQS
                     IIGVKTLAYLSGMGRNRRF"
BASE COUNT          586 a          310 c          187 g          640 t
ORIGIN      
        1 ttcggatttc ttgcaacaag agcatcattc aatgctactg aatctacaac gatggatatg
       61 ttatcttcat cgttgattcc accggataca atattactta taaaaatcgt aacatttttt
      121 ccaaaagaag ggatgtccgt gatgtataat gcccattttc cgatatttcc agttatttaa
      181 acctaacgaa tttttacaat agtacaagta tcacaatcat taatatataa ctggaatata
      241 aatctccaaa aattcactat agtacagttt tttatgatta aaatctccta tttctaccca
      301 ttccgcttaa atatgctaat gttttaaccc caataatgga ttggtttagt aaaaaatagt
      361 taaatattcg ttgtttaatt ggattttgtt tatatgggta tgagtgaata caggcaatca
      421 actcatcgtt cataagtatc ttagacaaat aggagtttaa atcaaattct ttattaaaac
      481 tagcatatct aactgccttt tgaatacaag tacgacagcg tcccaacaaa agtctccttg
      541 ttttgtttac aaatatctca ttttctaata tatagcttaa atcgccttca atttttttaa
      601 ctaattcaat attacaatcg aactcattat ctctaaaatt atgtgtcaat gaattattat
      661 tattacatct ataataatag atacaatctt taataaaact tgttattttt gaatgttgca
      721 aatatgaaat atggaatatg atatcttcag atatataatc cctttctgac ggaaacttga
      781 tattgtgctg ttgaattatc tcagatgaat acagcgcatg ccaaactgac attggcaata
      841 taaaatcgac tttataactc ggctcagtgc caatcattcc aagcaacact ttttcaatta
      901 tagcgttatt tgtaaatatc gtactatcat atcttgctgg ctttgaaata atattattat
      961 catcataata ttcataatat ccagcataac atgtgtctgc attactcctt tttataccat
     1021 ttaacattgc ttcaatataa ttagctgcaa caaaatcatc agaatcaata aaagcaacat
     1081 attttccttt tacatgttca agtcctgtat ttcttgccaa tcccaagcca gcattatttt
     1141 tatgaataat acgaatcctt gaatccttta atgcatagtc ttcacatatt ttattagatg
     1201 aatccttaga tccatcatcc acaattataa tatctaggtt cttatatgtt tgattacaca
     1261 ctgattccaa acaacttcct acatactttt caacattata tacaggtatg attacactaa
     1321 ttagatcact cgaattcatc cttctatcct ttcactcacg ttttctcaat tagtcaatga
     1381 cggcatcaac gattttctta aattctctga aaaaaataga ctctgatctt gatgatgtga
     1441 atgacatact atcttcttct agaaaaatta tttcctttaa attatttatt aaatcaataa
     1501 tatgatcctc gtaagtatgc tttcccttgg aataatgaaa atatactttt gtattacaca
     1561 atattgcaat tttatttctt aatatgatta tttcactcat tcattactgc cacatataca
     1621 caacttataa ccaccccctg caaagtttag ggtaagttat cagataatat cttcctcttc
     1681 atcatccaga atacaaaatc tatttattta tagaaatatc att
//
LOCUS       DACEXQ010000322         1695 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.333, whole genome shotgun sequence.
ACCESSION   DACEXQ010000322 DACEXQ010000000
VERSION     DACEXQ010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.333"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..252
                     /locus_tag="IXH04_002756"
     CDS             <1..252
                     /locus_tag="IXH04_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853293.1"
                     /translation="WAKKRILSMTERPIKYSASADQLPDIVISDYPIDKKAYDQTNTQ
                     IFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(278..718)
                     /locus_tag="IXH04_002757"
     CDS             complement(278..718)
                     /locus_tag="IXH04_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ1853294.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            878..1384
                     /locus_tag="IXH04_002758"
     CDS             878..1384
                     /locus_tag="IXH04_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1853295.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
BASE COUNT          609 a          251 c          332 g          503 t
ORIGIN      
        1 tgggcaaaga aaaggatatt atctatgact gaacgcccaa taaaatatag tgcatccgct
       61 gatcagttac ctgatattgt cattagcgat tatcctatcg acaaaaaggc atatgatcaa
      121 acgaatacac agatatttta ctggaatgga caacctacaa aaaatgattg ggcgcgtctg
      181 aaagctgaaa tcataaaaat caggaatgag aaaaatagat ccatattgga aaaattggat
      241 ttcgttaatt aagaaaagca gattgtttat gccaaaatta agacattgca aaaagattat
      301 tttctttaaa atcgacaaga tgaccagaaa gcaagagttc gttgagctct tgctttcttt
      361 tagttttaga aaggaacatc ctgattttat cttcgctaaa tccagtcaaa agttttgcag
      421 attggtcgta gataatcggt tttttcaata aagaagggga tgtacgaatc tcaatatata
      481 gttcactcaa actgagttca tctattctgt gaacaagatt tttataacta atagaatgag
      541 tagagagcat ttcggtcacg ccgttgtttg tcaattttag taagtggata aattcatttt
      601 tggttagtgg ttgacgaatt aagttctgtt cgataaacgg tatatcatgg acgataagcc
      661 aacgttttgc gcgtcttgaa gacgagcagc cagaacctgt atacaattta atcatcatac
      721 attcctccaa tagctaagta tttatgaaat aataacatga agaaatagtc gaaaagtgcc
      781 atttagaggt gtaaaataag aaaatgttgt cacgtataat ggaactagct agataagaaa
      841 tactattaaa tcaagaaaaa agaaagaagg cacaagtatg tcaaacacag ccgacgcaat
      901 agtagtgatc gatttacaaa aaggggtatg ttttgattct tcgccgatac acgaattaga
      961 aacagtgaca gcattagtga ataaaagaat caaaacatac gaaacgattg ggaaaccagt
     1021 tattttcgtt caacacgaag atgctcaatt agtgccacaa acaaaaccat gggagctatt
     1081 gtcggaatta tctattcctg agcacagtta ttttgtgaga aaaacccatg cgaattcatt
     1141 ttataagact aatttgagtg ttttattaac tgaattggac tgtcattcga tcgaattatg
     1201 tggggcacaa gcagagtatt gtatagatac gactgtgaag tttgctcatg gattaggata
     1261 ccagcttatg atgtgtagag gagcaacgac aacttatgat aatcctttta tgaaagcaaa
     1321 ggaaacaatc gctttttacg agaaaatttg ggataaacga tttctgacat ttttggagca
     1381 ataagttgtt tcttcgttta aaaaaagcag aatggatact ggtttatcca ttctgcttta
     1441 ttacgtaaaa tgagaaaata atcctgttct atttttatgg gataaaccat ttattttttg
     1501 tataaaaaat gtttgcgttt acatataatt ctcctagaaa cataaaaagg agtgaatagc
     1561 ttgagaacaa aacaagtatt aaagggagcg ttggtcatct tatttgctgt aagtacaagc
     1621 gtcccgcaat ggacagtata tggagagagc atttcttccg aatcggaatc tcgggaggta
     1681 gaaaccacag aaact
//
LOCUS       DACEXQ010000323         1658 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.335, whole genome shotgun sequence.
ACCESSION   DACEXQ010000323 DACEXQ010000000
VERSION     DACEXQ010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.335"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..378
                     /locus_tag="IXH04_002759"
     CDS             <1..378
                     /locus_tag="IXH04_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853296.1"
                     /translation="HLTAAELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNL
                     IKTTQLLKKYPGLLHTSIQFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKK
                     KVIVIDFTRNRNVLHQVAMEKAN"
     gene            437..877
                     /locus_tag="IXH04_002760"
     CDS             437..877
                     /locus_tag="IXH04_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853297.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            889..1557
                     /locus_tag="IXH04_002761"
     CDS             889..1557
                     /locus_tag="IXH04_002761"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAQ1853298.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
BASE COUNT          610 a          271 c          335 g          442 t
ORIGIN      
        1 catctgactg cagcagaact gcaaaaacaa atgcgttatc aaatagatga cgaaaattct
       61 ttttctattt ttagacgtca gcaaaagatt ctaaaaagct ttttaaaagt gatcagtaaa
      121 aaacggaatc tgatcaaaac gacacagctg ttgaaaaaat atccaggtct gcttcacacg
      181 tctattcaat ttcctcaatt gattacgatg atccatcgtt ttatacgtat gcaacagcta
      241 ccatctaaga agctgtttct tccattgacg gacacatttc gagtagtcaa cagagaagat
      301 aaaaagaaag tgattgtgat tgactttacc agaaatcgga atgtccttca ccaagttgca
      361 atggaaaagg ccaattaagg ctttttcttt ttggattttt ttgtcataat aaatattaga
      421 tgatggagga gataatatga tgcaaacatg gttagaaagg ctaatacgat ttcagcagaa
      481 ctatcacaaa cttatgcagg gaagatatgc gaaatttgat cagttgaatc gcggtctatt
      541 gatcgctgcg cttgtcttta gtttgctaag tcgttggttg ccctatagaa tcggacaatt
      601 gttgttcatc gtctttttcg gatggatgat atttcgtttc ctatcaaaaa aaatctaccc
      661 cagactaaat gagaatcaac gttatttgaa atatcttgat agagtcaaag aaaaatatca
      721 tcagataaaa aacaaaaagc aagtcaaaat ggatcgaaaa agttatactt tttttgagtg
      781 cccgaattgt catcaaaagc agcgtgcacc taaaggaaaa ggacgaatac gtgtgacatg
      841 caaaacatgt ggtataaaat tcgaaacgaa tgtttaaggg agaaaagaat gaaattagta
      901 ttattacgtc atggcgaaag cgaagcaaat tttgaaaatt actggacagg atggctagat
      961 gtcgccttaa cagaaaaagg aaaagaacaa gcagcagttg ccggtcaaaa aatgcttgaa
     1021 gccggacttc aattcgatgc cgtttatacc tctgttttga aacgtgcgat tcttaccgga
     1081 caagtcgcac tagaagaaat gggtcagtta tggcttccta taataaaaac atggcgtcta
     1141 aatgaacggc attatgggga tcttgtgggt aaaaacaaag ataagatgaa agaaatatac
     1201 ggaaaagatc aagtaaaaac atggcgcaga ggatatacgg aagttccgcc gttaaccgca
     1261 gaaaatagat ttgaccgcag atatgatcac ttggatccgc atttgatccc atacggagaa
     1321 agtctggaaa tgacagtgaa tcggatcatt cccttatggc aagatcatct agcaccaaaa
     1381 ctaaaagatc ggcaagattt actagttgtg ggtcatggta atagcatccg agcactgaca
     1441 aaatatttag aagatgttcc tgaagatcaa atggatacga tcgatatccc aaatgcacaa
     1501 ccaatccagt atacattagc agatgatttg acaattctgg acaaaaaaat actttaaaga
     1561 acacctttat ctaaaagtga gagatagaaa aaagaagcaa aggaatggaa caaaaggaac
     1621 atgaaaaatt atatatggga ctttgatggt acattgtt
//
LOCUS       DACEXQ010000324         1657 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.336, whole genome shotgun sequence.
ACCESSION   DACEXQ010000324 DACEXQ010000000
VERSION     DACEXQ010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.336"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            260..910
                     /locus_tag="IXH04_002762"
     CDS             260..910
                     /locus_tag="IXH04_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853299.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            1097..>1657
                     /locus_tag="IXH04_002763"
     CDS             1097..>1657
                     /locus_tag="IXH04_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HAQ1853300.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
BASE COUNT          529 a          199 c          337 g          592 t
ORIGIN      
        1 ctgctcgatc aaccttgaga actgtttttc ttacttctgg tcaagattct cgtggttggt
       61 ggataaggat ggttgataag ttcattggat tagctagtcg aaaacaacca ggtgtaatta
      121 atgtagaagt tttaacaagg ggatggagtg atttattcag tgataatcat aagttcataa
      181 atagtaattg ggatgtatgg ttggtatacg ttatacctga acttgaacta tcaagatttg
      241 gtttagaatt tgagaatcga tgtttataaa gggagggcaa aaaatgtgct tttttacaac
      301 ttttactatc attatgattg cagtactttt tatttttcta gatatagcaa agcgaaatac
      361 agcattttta ttatatcggg ttttacttcg agcaggtctc ataacattta tatcgatcgt
      421 tggatttttt ctattcacag ttattgtatt tatttggaga actacagcac caccattgcc
      481 agagattaca tatggagaat ttccgtttag tcttgaatat gaaatagatg aggaaagaat
      541 tttgattgaa gatacggtga ttgcagaatt tataggaagt tttagaggga atgtgacaac
      601 tcaacctaga cgaagttgga gtataaaact tttaaataat agtggagagt tgaaagcatt
      661 tcattatgaa ttcttaataa tagaaagtga agatgtaaca atcactttaa gtagtggctc
      721 tgctggatat tttatgaatg atggtggaag caagccacag ctcacagtcc gtgatcgtat
      781 tgcaggtttt atgcattttt ctcactatga cttagaagaa gctcgtaaaa tacttgcaga
      841 atatgggatc acattaatca attgggaata tgcgccaccg ataagaaatt ttttcaatga
      901 ttattattag tcttataaaa aacagaatgt aaaatttatg tgcgcgagta tttagttttc
      961 cattttttgt ttgatagata tatagcattg ttgaagcatg ggatagtggt gccaatttta
     1021 tcaatgaggt tggtcaggca gttaacataa ttggtgatgc tataaatcca tttaattgga
     1081 attggaggaa ttaggtgtgg aaacagtgag attatggata ttcatgaaaa attttcgttc
     1141 ggctttgaga tatgttattc tggaatcaaa atcaggttat tatctgattg acacagatca
     1201 ctatactttt tggggaggct acctttttcc gatttttaat tggataatcc cccataaggt
     1261 tatgaaaatt aacttgcctg cagaagaaat aaatagatta atactcaaca aagatttaca
     1321 aaggaaaaga gagaaagaac attctaatgt tgcttggata ggtccagcat ttgcagtagt
     1381 gacttcttca attgtcggtc ctatgctaga gagtatttat agtgattttt ctattggaat
     1441 acgttattta tttgttactt taagtatagc tgttcttttg tgcataaagg gttatgttag
     1501 caagaagaat aaagatattt taacactaat cggtgtaaac aatttatcta gtactaaaat
     1561 tatactgttt cctgaatcta taaaagaagg atcaaggttg ttgataattt ttataatgct
     1621 tatactgttt tctgttttat ttggggtgat gtttata
//
LOCUS       DACEXQ010000325         1634 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.337, whole genome shotgun sequence.
ACCESSION   DACEXQ010000325 DACEXQ010000000
VERSION     DACEXQ010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.337"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            1..565
                     /locus_tag="IXH04_002764"
                     /pseudo
     CDS             1..565
                     /locus_tag="IXH04_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
     gene            695..1435
                     /locus_tag="IXH04_002765"
     CDS             695..1435
                     /locus_tag="IXH04_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853301.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT          590 a          258 c          335 g          451 t
ORIGIN      
        1 aaaaccagtt gtcttgtttg gggaaaattc tgaatgatat gattatgtag acagcgataa
       61 tgaatatggt acagcaaaag caacgcaata tgccttagaa agagattatc aaacgattat
      121 ttatatcggt atcgaagtgg acgagccttt tgaaatttca agggaaactg gttatttatc
      181 tgtgatgaag gaagttgatc ttcctagtaa aatcttgcgg tttccaaatc attcgacaga
      241 agtagaaaga tatatgacag aaaactgggc agattttaca gataaaactt gttttgtatg
      301 tagttcagat cggctagcac taggagcagt aagagggatt ataaatgctg gaggacactt
      361 gcccgaagaa tttggtgtga ttggatttga tggggtattt ttagaccaaa tcagttcacc
      421 taaattgaca acgatcaagc aagatattat acgtatgggt gaagtatgtg gagaaatgtt
      481 gttaaaaaaa atagaagaaa aaggggcgag tcaaggttat cgtcgttttt ttcctgaact
      541 agttatcaga gagacaacga attaagtctt attactatat atatatttat gaaattcatc
      601 gctattttct taaaataatc agcgcattgc tactgaaaac aatgaatctg tgctattatt
      661 gagaaaagtt ccatttttga gaaggatggt ttgaatgaaa gaaatcagac gaaaacattt
      721 ccgttttcct gcgtacgatg atgagttagg cgtaaaatta accaacgaca agaagcgagt
      781 attatttgac ggacaagaag atcttttgac tgaacagaat cagtccccgt ttgaacagat
      841 gaaagaattt catttcacga acacgaacac cgagaaaatc cgtcgcaagc gtcgtgcaac
      901 aaatggattg aagtcagctc cgcgtaagga aaatttggaa aagcataaag gaaaattacc
      961 ggattatacc ggccattacc aatcagaaat cacatcgaca ggacgaaaaa aactttttgg
     1021 tgattcctct cgttctgtca gacaaagtga aacaaggaca gagtccaagc atactacacg
     1081 aaacgcatct gttgacacga aggtgcggga gcgttcttat tttgttccta aatatgtacc
     1141 cgcatcgatt atcccagacc ctagagaaag caaaatttct gaggatcatt taatcgaatc
     1201 gatgaaaaaa acggatgact cttatctgtt gttcgataca gaaccgacgc cgtatcaaga
     1261 aaaaagaaat ggtgagccgt ctgtacgaac attcaatcga acaaaaacgt tagaggaaac
     1321 aaagcagtca gaagaaaaaa aatcgaaaaa aagccatggt atcttagaac gctcattaca
     1381 aggattgatc gatgatcaag gaaactcttt gggagaaaat agctatttta aataagtgat
     1441 atacaaattt atattcggct acagttggaa aggaaacgct gcggcgtttc cttattttat
     1501 actctatccg aaagtttata tcgtatcctc aaattttttt gagtttaagc gaattttttc
     1561 tcccaataag taagaaaata atggcaaaat aaaaaaacaa actattttcg cagatcgttt
     1621 gcaaaaatag ttta
//
LOCUS       DACEXQ010000326         1631 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.338, whole genome shotgun sequence.
ACCESSION   DACEXQ010000326 DACEXQ010000000
VERSION     DACEXQ010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.338"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..302)
                     /locus_tag="IXH04_002766"
     CDS             complement(<1..302)
                     /locus_tag="IXH04_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HAQ1853302.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
     gene            661..915
                     /locus_tag="IXH04_002767"
     CDS             661..915
                     /locus_tag="IXH04_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1853303.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            916..1233
                     /locus_tag="IXH04_002768"
     CDS             916..1233
                     /locus_tag="IXH04_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ1853304.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          556 a          259 c          311 g          505 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttatatc aactcatttc ttgtatagac tgattgtaac atgggacaaa ataaaaaggc
      361 aatacgcctt agttgtcggt cttcgagtta ccaacggtaa ccctcatacc gaacggcatt
      421 catcacccac ctatagagga tgggcgactt ctgcctaatt ctgttaaatg gatgttaacg
      481 gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt tttcagcgtt
      541 ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga aacaccgttg
      601 gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga gtgtgataac
      661 atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat ccttccaagc
      721 tctcttggat taaaaagcgg agacattatc caagccaaac aagaagacaa cttattcata
      781 ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga aagtttccaa
      841 gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt cggaaaatac
      901 ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt tcagaccagc
      961 cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc cagcataaaa
     1021 agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc aactatgcat
     1081 gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt gatcggcaaa
     1141 agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt aggacgtatt
     1201 ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag tcgctcatcc
     1261 tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt actcgaagat
     1321 tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa ggtctaaaga
     1381 tacaatcatt agtctagaga aaaattaaga accgtaggaa ctacggggat cgcttggtga
     1441 ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat tcccaaaaat
     1501 ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat tgacgtaact
     1561 tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa gaggaagtcc
     1621 aatatatcta t
//
LOCUS       DACEXQ010000327         1607 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.339, whole genome shotgun sequence.
ACCESSION   DACEXQ010000327 DACEXQ010000000
VERSION     DACEXQ010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.339"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            373..972
                     /locus_tag="IXH04_002769"
     CDS             373..972
                     /locus_tag="IXH04_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ1853305.1"
                     /translation="MKTVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLADVLEISRGRVVVKDRLAMKEQKTAKHNSVYISNKLRKTILEYVQQEFPDQ
                     LLQQDFDGYLFPSRKGTNEPLNRQSLWRIIHDAGTAIGLKDIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            1036..>1607
                     /locus_tag="IXH04_002770"
     CDS             1036..>1607
                     /locus_tag="IXH04_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853306.1"
                     /translation="MSTLYIIGNGFDLSHGLKTRYTDFSKWVEKSHSEIFDKIDTMIN
                     ENNEVRSTADDKIGWFNFESSLHELIQSPVFEDYVEESKDYLIGYGHEDWSDSAHHDF
                     QNMVYDYVVSLEELQQLFKEWIRKEIQPTIEHKIPKYQFSSDVLFFTFNYTDVLEILY
                     KQPKENIYHIHGSYDDIIIGHSENFDNFIG"
BASE COUNT          568 a          244 c          311 g          484 t
ORIGIN      
        1 atatctactt tattggatcc gtaacatgac taaattctga atacttttta aagcttatca
       61 tttggtaaaa ctgaatataa gttaattata tagactgaaa attttgaatt ttcagaatag
      121 aataacttaa aagtactgac aaaatttttt aaaggggcaa tattaatgtt ctgagataat
      181 caaatcctac cgaaaatatc aaaaatccaa tgtgacacaa tcagattgtg tctcattcga
      241 gggaatcaca caatcccacg tggctctagg cagaattgaa tttttgcttg gggctttttc
      301 cttatattga ttttgaatga gacttattat atgttataat gcaattaagt atcattggag
      361 agggcgaaga aaatgaagac ggtagaacct attcgagaca aaaaaaagat cgatgcgatg
      421 aaggcaatct tggcttcggg aaaatatggc caacgaaatt tggttctttt ttcgattggg
      481 atcaacaccg cctatcgcat ttctgatttg cgtcaattga agctggccga cgtgctggaa
      541 atttcacgcg gtcgcgtggt ggtcaaagat cgattagcga tgaaggaaca aaaaacagct
      601 aaacataatt cggtctatat ttccaacaaa ttgcgaaaaa cgattttgga gtacgttcaa
      661 caggaatttc ctgatcaact ccttcagcaa gattttgacg gttatttgtt ccctagtcga
      721 aaagggacga atgaaccgct caaccgccaa agtctgtggc gaatcattca tgatgctggc
      781 acggcaatcg gattgaaaga cattggccca cattccatgc gaaaaacgtt cggttacttt
      841 ctctataaac aggggaccaa aacagaaatc attcagtcct tgttgaacca ttcttctcag
      901 cgcgaaacat tgcggtatat tggtattaca caagaagaca aagatacagc tgtaaaaagc
      961 ttagatttat aatataaaag ttaagtttaa acatggatag ttttaaatta gtagattgag
     1021 tgttgggagg aaagaatgag tacattatat attataggaa acggttttga tttaagtcat
     1081 ggtttaaaaa ctagatacac tgatttttca aaatgggttg aaaaaagtca tagtgaaata
     1141 ttcgataaaa tagacacaat gataaatgaa aataatgagg tgagaagtac agctgatgat
     1201 aagataggtt ggtttaattt tgaaagtagt ttgcatgagt taattcaaag tccagtcttt
     1261 gaagattatg tagaagagtc taaggattat ctcataggtt atggacatga agattggtca
     1321 gattctgccc atcatgattt tcaaaatatg gtttatgatt atgttgtttc tctagaagaa
     1381 ttacaacaac ttttcaagga atggatacgc aaagaaatac agccgacaat agaacataaa
     1441 attccaaaat accaattttc gtccgatgta ctattcttta cattcaatta tacagatgtt
     1501 ttggaaattc tatacaagca accgaaagaa aacatatatc atattcatgg cagttatgac
     1561 gatataataa tcggccactc cgagaatttt gacaacttta taggaga
//
LOCUS       DACEXQ010000328         1572 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.340, whole genome shotgun sequence.
ACCESSION   DACEXQ010000328 DACEXQ010000000
VERSION     DACEXQ010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.340"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..424)
                     /locus_tag="IXH04_002771"
     CDS             complement(<1..424)
                     /locus_tag="IXH04_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAQ1853307.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQI"
     gene            complement(533..1564)
                     /locus_tag="IXH04_002772"
     CDS             complement(533..1564)
                     /locus_tag="IXH04_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ1853308.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          489 a          272 c          275 g          536 t
ORIGIN      
        1 gaatctgttt tgattttgtt ccatttgaaa tttctctcac tcgctttttt gtctcatcat
       61 caaataaaat gaaagggtct tctaaacgat cgataaatac ttccattgtt gttgttggta
      121 gatgataaga agtcatgaca ggcactgcac caaggtaaga cacagaaaca gctagaaggt
      181 atgtgtcaaa agcggaactc ttataaataa taactttgtc tccggatttc actccaagat
      241 gagccaattg ataagcccgt ttcaacacac tttcgcagca ttttttgtaa gttgtttcaa
      301 gtcctaattc tgggaaagct gaaagtggct catcaaagat aatcggggtc tcggaaaagt
      361 tagctgcagc ttcttgaaaa ttcgtaaata agttcaaagg ggaatacgtt atggtctcca
      421 tcattcacac tccattatct tttgttaagt aatatacaaa ttcaagataa tcgtaacata
      481 atcctttata taataacata gtttattgtg aaaaaaagat catacatttg ttttatatgg
      541 aagaaacccg aacgaatgcc gctaattcgt gataagaaaa atcaaccatc ttttcccaga
      601 tatcttgaaa agaatgggat gagaggtatc gattaccaca taaataattt accgcgtcag
      661 catcatctaa atatagttct gcataataac gatatgtttt tctgcatagt tctggcgata
      721 cgtttttata tactgtaatt ttgctcaaaa tttggcgaat agtccgagga gaaatttgct
      781 gatcatgtga ggaacgaaaa acaaaagaat tttcgtccgt attttgtgct gttaacttaa
      841 aatagttctc cagaaatggc atcgcttctt ttggtattgg tactttattc agtctgtttt
      901 cttttctatt cagattaaga atctgcttgg tcaaagatac atctttacgt ttaagttcta
      961 ctacttcatg gatctttaat ccgcaataca gaagaatggc agtaattgca aggttcctcg
     1021 gtctattctt ttcttcaaag ctagcttctc tgcttgtttg aaagccttca atcgggataa
     1081 acaaatgctc aaaatctttg aaaagttctg catcgatata tcgcggagct gctttggttg
     1141 tttccggtaa acttgtaccc aaaagagaaa tctcttcttc aatcagtcct ttttgataca
     1201 aaaatcgtaa aaaatgattt aatgaatatt gcttccttga aatcccgtaa tagctatttt
     1261 caaacaattg ctccgtttct tggttcgctg tagtttggaa cgaacgagta tgtcgataga
     1321 ttttagctaa gaaattctga taatcttgtt tttctaaatg gataatcagc tgatcatcca
     1381 cttcttcaga aaatactttt ctcaaataat cagtaaacag atgaaaatct tttgcgtatt
     1441 catagcgact gcctaatgaa agggaagaac gattttcttc taaaaactgt tgaaagacgg
     1501 aagggagcaa gtgtgtacgt tgctttaact tttcttgcag ttgttttcta gttttgctgt
     1561 ccattacaaa tt
//
LOCUS       DACEXQ010000329         1551 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.341, whole genome shotgun sequence.
ACCESSION   DACEXQ010000329 DACEXQ010000000
VERSION     DACEXQ010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.341"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..175)
                     /locus_tag="IXH04_002773"
     CDS             complement(<1..175)
                     /locus_tag="IXH04_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAQ1853309.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFS"
     gene            complement(193..513)
                     /locus_tag="IXH04_002774"
     CDS             complement(193..513)
                     /locus_tag="IXH04_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1853310.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            812..>1551
                     /locus_tag="IXH04_002775"
     CDS             812..>1551
                     /locus_tag="IXH04_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAQ1853311.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEG"
BASE COUNT          573 a          266 c          246 g          466 t
ORIGIN      
        1 cgctgaaatg tgcaagttca tgagataaat gaaaggcttc tgcagtttca ccgtatagat
       61 tttcattcaa aaaaatgatt ctgggttttg gatagtagaa accaggctct ttcatctcca
      121 tataaactac tttcaaattg tattcgctca acatttcttt caatttcaaa tacatacaaa
      181 ccatcactcc aactattcat tttcctctaa agctttagca attgcaatcg ctttacgcat
      241 tgtctcctta gatatttctt ttccatcaaa agaaaaaaca gtatcgtctt ctgataaatc
      301 cacatgttta ggggtttctc tttcttctct acctagaagg tagtctacag agacatcgaa
      361 atagtcagca atccgactta attcatctga attaggagta ttatttttcc actttgctag
      421 ataaccgtta gaatatccaa aattaatttc taactgccgt atagatatct ttttcctctt
      481 cgccaagtct tttattattt cataggtatt catcgataaa acaacctttc taaatgttta
      541 caaaaaaagt ttagaaaaat acgcagaaat aatttgacta attctgagta atgagctata
      601 ctatatcttg taaacagatt taacaactaa aaagacaaca aaaaacacta ttgatatgta
      661 aatgcagacc gccaagaaag ctttaaaatc aatgttttta tgtcttattt aattatgctc
      721 ttagtataga gtattactca gatactgtca attgaattta gaaaaaagtt gttaaatttg
      781 tttacgaata taaaagaaag gaatgagaaa tatgaacaca ccgcaaattt ttaatttcga
      841 acaaaacgaa gttcggacaa ttttagtaaa tgatgaacca tattttgtag gcaaagacgt
      901 tgcgagtgtt ttgggttatt caaacactaa agatgctttg tcgcggcatg tagatttgga
      961 agataagatg gggtcgcgaa tcacgacctc aggtcaatca agagagatga caatcatcaa
     1021 cgaatctggt ttgtacagtt taatcttaaa atcaaaactt ccctctgcca aaaaatttaa
     1081 acgttgggta acaagtgaag tgttgccagc aattagaaaa catggaggtt atctaactcc
     1141 agaaaaagta gaagaagctt tgcttaatcc agatacaatc attcaattag caactcaact
     1201 aaaagaagaa agaactggaa gattaatcgc agaacaaaag attgcagagt acgaacctaa
     1261 aatctcctat ttagatagca tattatcttc tacagattca gtaacaatta gtcagattgc
     1321 agcagattat gggatgtctc cacaacagat gaataaatta cttcataaac taggtattca
     1381 gaaaaaagtc ggtaaccaat ggttattgtg caaaaaacac atgaaccaag gatacacaaa
     1441 atctcataca actgagatcc cgaaagccga tggtggcact aaaattgtaa tgaataccaa
     1501 atggacacag aaagggcgtc tatttatcta cgaattacta aaaaaagaag g
//
LOCUS       DACEXQ010000330         1532 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.342, whole genome shotgun sequence.
ACCESSION   DACEXQ010000330 DACEXQ010000000
VERSION     DACEXQ010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.342"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(62..280)
                     /locus_tag="IXH04_002776"
                     /pseudo
     CDS             complement(62..280)
                     /locus_tag="IXH04_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(546..740)
                     /locus_tag="IXH04_002777"
     CDS             complement(546..740)
                     /locus_tag="IXH04_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853312.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
BASE COUNT          538 a          234 c          205 g          555 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattgta ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      961 aagaagatat atatcatttc ttaattacct actaccttaa accaaaattt aagtactacc
     1021 taatttaagt attacttaaa caatctaaaa aatagttatc ccaattgtta tgcttttata
     1081 atacgattat ttgtaacaaa atgaatatta tataattaaa cactaccata tttaggtagt
     1141 gtttaattat tagcaaaaca aaaaatattt attctattct cttaacttca actaagttat
     1201 gctgcacacc tttatataaa tcatatgcaa ctcattagct agacaaacta caagcatgta
     1261 gttttatctg ttaattatgt actacctaaa taaagtagtt tataattagc aagtatatat
     1321 atttaaaaat ttaagattaa tttttttatg acttgtacaa atttatctga tattctctta
     1381 ttaagaacta cctaatttgg gtagttctta ttttttacat tttaagtagt atttaattgc
     1441 aatttggtag tattttttta ttatcttatt cttagaacgg tagtacttaa tataaatata
     1501 ggtagtatct aatttaagta ttaatggtag ta
//
LOCUS       DACEXQ010000331         1526 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.343, whole genome shotgun sequence.
ACCESSION   DACEXQ010000331 DACEXQ010000000
VERSION     DACEXQ010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.343"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            290..1108
                     /locus_tag="IXH04_002778"
     CDS             290..1108
                     /locus_tag="IXH04_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ1853313.1"
                     /translation="MDTFQGTTFTVGNFKGGVGKTKIVSMLAYDNAMIRNRKTLIIDL
                     DPQANLTQVIARTFGITDIKTTITNGVANGELASSIIKVNENLDLLACDTSFRSFSAF
                     ANKHENELDQVTVLKKLLLPIKNNYQEIFIDVPPTISEFSDNAMAASDYSIISFQTAE
                     ESLEGVNKYVNYQKFMVDRYDLDLQIIDIVPCMVEPNDDFDEEILEEAIEKYGTVVSK
                     NLIHYQKRLRRYSKIGIHLRKYKNGNFDQWDYKAHQVFINILAELDARQEFLNK"
     gene            1127..>1526
                     /locus_tag="IXH04_002779"
     CDS             1127..>1526
                     /locus_tag="IXH04_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853314.1"
                     /translation="MPKDFDIPKMMENGEFEPLLPNNKRQDVKISGEYNFSNSALSNT
                     RNITLKKRGRPVSIPDKRNKVSVPKKISPALNSKLSILQDYMTELQSEKGRISFEKIV
                     NTLADSYIQHRLGVAKEEHIREEIQEEFEKL"
BASE COUNT          620 a          210 c          248 g          448 t
ORIGIN      
        1 agaaaatgaa tatgatcaat tcaattcaaa tcagcagcga aaagtaaaga aacagataga
       61 ctagaatagc tattttcgaa aagcaatatt taaggtataa gttatacctt taataataaa
      121 agtataagtt ataccataaa tatatatggt ataacttata cttttattat taaaaaccgc
      181 tatatccaaa cgtttttaga tattattatt taagttatac ttttaatgtt gaccatttcc
      241 gatatataag ttatacttta attatcaatt aaagattggg aggatttaca tggatacttt
      301 tcaagggact acatttacag ttggcaattt taaaggagga gtaggtaaaa ctaagatagt
      361 cagcatgctt gcttatgaca atgcaatgat aagaaataga aaaacgctta ttattgattt
      421 agaccctcaa gcgaacttaa cacaagtcat agcaagaact ttcggtataa ccgatatcaa
      481 aaccactata acaaatggtg tcgcaaatgg agaattagca agtagtatta ttaaggtaaa
      541 tgaaaatctt gaccttctgg catgtgacac tagttttcga tcattctccg cttttgctaa
      601 taaacatgaa aatgaattag accaagttac tgtcttgaaa aaattacttc ttccaataaa
      661 gaacaattat caagaaatat ttattgatgt accaccaacc attagcgaat tttccgataa
      721 tgctatggca gcttcagact attcgataat ttcatttcaa acagctgaag aatccttaga
      781 gggagtcaac aaatatgtaa attatcaaaa attcatggtc gatagatacg atcttgattt
      841 gcaaattata gatattgtac cttgtatggt agaaccaaat gatgattttg atgaagaaat
      901 cctagaagaa gcaattgaaa aatacggaac cgtggtatcc aaaaatctca ttcattatca
      961 aaaaagattg agaaggtatt ctaaaattgg tatacattta agaaaataca aaaatggaaa
     1021 ttttgatcaa tgggattata aagcacacca agtatttata aatattttgg cagaactaga
     1081 tgctagacaa gagtttttaa ataaataata aggagttgca taagtaatgc ctaaagattt
     1141 tgatattcct aaaatgatgg agaatggtga atttgagcca ttattaccca ataacaaacg
     1201 ccaagatgtg aaaataagcg gtgaatataa ctttagcaat agtgcattga gtaacacaag
     1261 aaatattaca ttgaaaaaaa gaggccgacc agtttcaatt cctgataaaa gaaacaaggt
     1321 atcagttcct aaaaagatct ctccggcatt aaatagcaaa ctctcaattt tacaagacta
     1381 tatgacggag ttacaaagtg aaaaagggag aatttctttc gaaaaaattg ttaatacatt
     1441 agcagactca tatatccagc atagactagg agtagcaaaa gaagaacaca taagagaaga
     1501 aatacaagaa gagtttgaaa aattac
//
LOCUS       DACEXQ010000332         1511 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.344, whole genome shotgun sequence.
ACCESSION   DACEXQ010000332 DACEXQ010000000
VERSION     DACEXQ010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.344"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            108..1040
                     /locus_tag="IXH04_002780"
     CDS             108..1040
                     /locus_tag="IXH04_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HAQ1853315.1"
                     /translation="MLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLD
                     EIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            1112..1468
                     /locus_tag="IXH04_002781"
     CDS             1112..1468
                     /locus_tag="IXH04_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853316.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQDMRV"
BASE COUNT          668 a          173 c          236 g          434 t
ORIGIN      
        1 atgaaataaa tcaaaagtat gatgaaacat taaaaaatgt ctattcttca cttatcgttt
       61 caaatataga agaaacttca tcttctgaaa taaaaaataa gctttctttg ttaaaattaa
      121 aagaaaagga atatattatt gacgatgctg tttctgtccc tcttatttta aataaacgag
      181 agaaaaataa tttaaagaaa aaaatgctca ttccatttga ttcagaagtg aaaggattac
      241 tcaataaact aactatttct aatatagact cgactaggga aaaaatcata aaaatatttg
      301 ataaaattaa taatttattc aagaatgaca atgctgaatt aaggaagggt cttttagagt
      361 ttaaacttca agaactagaa cttcattact cttatttagt gaaaattacc gaagaaaaag
      421 aacaacaaaa agcaataaaa gaacagatga tagaagagga aaaagttcgt agagaaattg
      481 atcgtgaaaa gaaacgtatt gataaagaac aacgtcaatt caattctgaa atttccaaat
      541 taatcgcata tatgcaaaaa gctaatgcag atgtggaaaa agaattatat gcaaataaaa
      601 ttcaagaact ggaagaaaaa ttaaaagaat tagaagttat acgtgaaaat gtattacaaa
      661 gagaattaaa tactagagcc ggttatgtgt atgtaattag taatattggg tcttttggtg
      721 aagacatata taaaattggt atgactagaa gattagaacc tatggataga ataaaagaat
      781 tgagtagtgc atcggttcca tttgaatttg acgttcatgc gatgattttt tcagaggacg
      841 ctccgtcttt ggaaactaaa ttgcataatc attttagaaa acaagaagtt aataaaatta
      901 atcaaagaaa agaatttttc aaagtttcac tagacgaaat agagaaagtt gttttagaaa
      961 attataatgg aacagtaaca tttacaaaat tagctaaagc tgaacaatat agacgatctt
     1021 tagaattgtc aaaagattaa atatataaaa agccttcggg ctttttcttt taacaattaa
     1081 taggaacgta tattcgaaag gaatagaaat aatgtggtta gaaaaaataa atgacaaaaa
     1141 atttaagtac accgaacgtt atacggatcc cttaactgaa aaaaagagaa aagtgtccgt
     1201 tactttatct agcaattcac gccaagcttg gaatcaagct aacttacttt tgaatgaaaa
     1261 aatcgcagaa aaaataaaac gaaatgaaga attaccacta acttttggag aattaaaaac
     1321 taaatgggat gaaaaataca agccaactgt aaaagaaagt tcctatcgaa caactcaggt
     1381 ttatctatca ttaatttcaa aatatataaa agatgatgta cttgtaaaaa acgttaatag
     1441 taatcttata caagatatga gagtataaaa tattttgtgt aaatgaaaaa atccatacaa
     1501 aaaaggaagt c
//
LOCUS       DACEXQ010000333         1501 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.345, whole genome shotgun sequence.
ACCESSION   DACEXQ010000333 DACEXQ010000000
VERSION     DACEXQ010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.345"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(81..1313)
                     /locus_tag="IXH04_002782"
     CDS             complement(81..1313)
                     /locus_tag="IXH04_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ1853317.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSANPDAFAGVDSHRVASYQSAAGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVIDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
BASE COUNT          409 a          320 c          276 g          496 t
ORIGIN      
        1 gaaataaagg atctaagcca atagcttaga tcctttattc tatgctttat attatttttt
       61 gcatgtgtgc gaaagtatac tcaagcccaa tcgccatttc ggaaaatcgg tactgtagag
      121 ccatcttcac ggattccgtc gatatccatc ttgtcagatc ctaccatgaa atctacgtgg
      181 gtttgacttc tattcaatcc agcttctgct aattcttcat cggacatttc tgtaccgcct
      241 ttcacactga aggcataagc agaacctagt gctaagtgat tcgatgcgtt ttcatcaaat
      301 aatgtattga aaaagatgat gccagattga gaaataggtg aagggtcagg tacaagtgcg
      361 acttctccta aacgacgcgc accttcatct gtatcaagca gttttgctaa gacatcttct
      421 ccttgttcgg cagaaaaatc aatgactttt ccatctttaa aagtaaattt catacccgag
      481 atgatggtgc cagcataact cagtggcttt gtacttgaaa tgtaaccatc tactcgatga
      541 ctgtcaggtg ctgtgaacac ttcttccgtc ggcatatttg ccatgaattt ttctccacga
      601 gcattatagc ttccagcgcc ttcccaaaga tggtttttag gcaatccgat aatgatatct
      661 gtaccgggag cagtatagtg cagggcagaa aattgttctc gattcaattc ttctgctttt
      721 tttgctaatt tttcatcatg cttcttccat gctaagacag ggtcttcttc atagacacga
      781 gtcgttttga aaatctgatc ccataatgcc tcaacttgtt tttcttctgg cagttctggg
      841 aagacttttg ctgcccattg tttaccagcc gcagccacga cagtccaact gactttattt
      901 gcttgagttg ccttgcgtaa attcattagt gctttgccag ccgctgattg ataagacgct
      961 actcgatggc tatctacacc agcaaatgca tcgggattgg cagaaacaac actgatcctg
     1021 cttgctcctt tttcaagcca ttcatccgct tgatcgattt tagattgtgg tacattctca
     1081 atacgatccg ttgcagcatg taaaagaaat tctctttgga tctggtcgtc agtccattga
     1141 acgataacct cagcggctcc tagtttataa gcttcttgtg tgatcaatcg agcaaggggt
     1201 gcctgttcca cgctgatctg taaaacaact gtgtgtcctt tttctgtggc tacgccagtt
     1261 tctgcaatta agcgtgcata tttttttaga agttcattaa aatcagataa cataataaaa
     1321 attcctcctc accatttttt ctggtaacta ttagcatgat accatttaat tatgattcaa
     1381 acaatgaggc ctttaaataa aaaaaagagg ttattttttt aaggttaagt ttcatgatat
     1441 atgttactac ttgctatttc agcttgtgaa gaaaatcaaa aactaatttt gcatatcaaa
     1501 a
//
LOCUS       DACEXQ010000334         1498 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.346, whole genome shotgun sequence.
ACCESSION   DACEXQ010000334 DACEXQ010000000
VERSION     DACEXQ010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1498
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.346"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..691
                     /locus_tag="IXH04_002783"
     CDS             <1..691
                     /locus_tag="IXH04_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HAQ1853318.1"
                     /translation="DVIIGRKERPVSCGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQ
                     EKRGKQQYQRSIHDRPDNYPDFNSEFGHLEGDTIVGIHHKSAVITLVERLSKVIITIK
                     PNGRKALDIETALNQWFSRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTP
                     SQRPLNENSNGILRRNGLPKSIDFREVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLS
                     YVQEAFYSSLI"
BASE COUNT          475 a          293 c          215 g          515 t
ORIGIN      
        1 tgacgtcatt atcgggcgaa aagaaaggcc tgtttcatgc ggtatgagaa cactttatcg
       61 tttattttct aaaggaatat ttgatattga cacactaccg atgaaaggta aaagaaaacc
      121 caatggccat caggaaaaac ggggaaaaca acagtatcag cgctcaatcc atgatcgacc
      181 tgataattat cctgatttca attctgagtt tggtcacctt gaaggtgata cgatcgttgg
      241 cattcatcat aaaagtgccg tcattacttt agttgaaaga ttatctaaag tcattatcac
      301 gattaaaccc aacggccgta aggcattaga tattgaaact gcccttaatc aatggttttc
      361 tcgcttccct aaaaacttct ttaaatctat tacgtttgac tgtggaaaag aattttctaa
      421 ctggaaagcc attagtaacc aacatgatat tgatatatat tttgcggacc ctggaacacc
      481 ttctcaacgc ccattaaacg aaaattctaa cgggattctg cgtcgtaatg gactgccgaa
      541 atcaatagat tttagagaag tgaatcagac atttatttcc agtgtcagca atcaacgcaa
      601 tcatattcca agaaaatcat tgaattacag aacaccaatc gaaatatttt tgagctatgt
      661 acaagaagca ttttattcta gcttaatttg acaaatcata taatttaaaa ttaacattga
      721 ttctcctatg aaaatatctt ttacctttct cctgtgaaaa ggaaatttcc ttttttatag
      781 tgttattgat ataatcagaa tattcatcaa actctgtatc tggagttaaa aatctacagt
      841 aacatctata agctttgcta gtaatcttct tgggttctct ctccattgtc aagcaacatc
      901 aaaacaccta cttactattc gattcttttc tgccaaaaac gagtcagcat cactcacaac
      961 ttatgtgttg ctgactccga ctagtccttt gttcaatatc aacttattaa acagatataa
     1021 tatttttctt tatttcgtat actccaagac ttccatcttt ctttcatctt atctctgaaa
     1081 aaaacacagc aaagtgccct taataaatat acattagaat ttgcaaattt tttcatctta
     1141 agatatatcc tattcatatt ctttacaatc ctaatgggtg attacaaatc tatttagttc
     1201 aagttctaca ttataaacac ctttcttgat agtatcatct gctgcacaag cgatgtcgat
     1261 ccacttatta ggggatttac gtgctcaaaa gtggctattc cttttgggaa gtccatccat
     1321 aataatggtc catggatttt tttagggtct tgtcgttcaa atttctcaaa aactttatta
     1381 tacttccata gttttataat tatttggttt cactttggct cgttcaaaca ccaacttgta
     1441 tttcttttca acgttcgaac tggagccccc cctataatct cattattatt ttcatgac
//
LOCUS       DACEXQ010000335         1495 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.347, whole genome shotgun sequence.
ACCESSION   DACEXQ010000335 DACEXQ010000000
VERSION     DACEXQ010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.347"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            57..410
                     /locus_tag="IXH04_002784"
     CDS             57..410
                     /locus_tag="IXH04_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853319.1"
                     /translation="MAKIDDYRSDMIEYIELFAKNSNNNIIIQTGGATICGTPIDFDA
                     EVKVNPLIDAMMDSFAEFRSKKIDDIEKDDEQSLVVKSIFLKDVTIIGERTTNIPFLV
                     VFADQISAISLGNLE"
     gene            complement(810..1277)
                     /locus_tag="IXH04_002785"
     CDS             complement(810..1277)
                     /locus_tag="IXH04_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HAQ1853320.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQPR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
BASE COUNT          472 a          267 c          247 g          509 t
ORIGIN      
        1 gtataatttt atttatcagc gagtggtccg ctgaaataat ttaaggtggt aaaaaaatgg
       61 caaaaattga tgattaccgt tcagacatga ttgaatatat tgaactcttc gctaaaaatt
      121 ctaacaataa tattattatt caaactggag gagcaacaat ttgcggaact cctatagact
      181 ttgatgctga agtaaaagta aatcctctta ttgatgctat gatggattct tttgcagaat
      241 ttcgctcaaa aaaaattgat gatatagaaa aagatgatga gcaaagtttg gtagtcaaat
      301 ctatctttct gaaagatgtt accatcatag gcgagagaac tactaatatc ccatttttag
      361 ttgtttttgc tgatcagatt tctgcaattt ctctcgggaa cttggagtaa caatagattc
      421 atttaaatta tctatattga ttaaattgct aatctctcgg ctattacaaa tagctgagag
      481 gatttctttt atttctcctg aagtaccttc aatcgataat ttcatattct taccctccaa
      541 cacataattt aatagacagc agcatacgaa gaattcagaa ggagttgagt tcacatcctt
      601 attcttaata tttccgctgc tgtctatcga ggcttaattt aaaacgatga gggagatttc
      661 ctcccttaca ttttattttg tctaagacct atcactaatc tttcgacact atcataatac
      721 aacgttgaat aggtaagtga ttggtataaa aaaggtataa aatggaaacc aaattggtaa
      781 taaaagggta taaaaagtgt aaaaactggc tacttgaaag caaccagttc taacgatgaa
      841 gcaaattgga tgataattct gtttgattct actttaaccg attcttcgct agtattattc
      901 ctttgagcag ttacataaat gggcagacca ttgatataac gatcatagaa tatcttcttg
      961 cgcctttcag tcacatcagg cttatgcgga tgctgtatcg ctgaatagcc tcgaacaaac
     1021 aatttatgca gatactcaaa ttcttcctgt gcttcttctt tatcgatcag cattctttct
     1081 gcttcaaata tatgatcagc tgtagaaggt ggaaccaagg aataagatgc tgtcactttt
     1141 ggttctcgag gttgccctac tctacatcta gctgatagat atgctgaaag aaacacagcg
     1201 acattatgtt ttgtgcgttc catatcaaca tctttcgcgc ttggtgtctc atatttcttt
     1261 acatcgaaaa gtaccatcct ctgattcccc caattatgat ataatactta tgtcggaaat
     1321 attattcata gtcggaggaa tccgactttt tttattttgt acgtgaaatg agttctcctg
     1381 atttatacgc ctcagcaaat tcaaccaaag ctactgcctt cattctctcg atagttctat
     1441 cggaatatcc taatttttct cctatttgat acattgacat cttattagga aaaca
//
LOCUS       DACEXQ010000336         1471 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.348, whole genome shotgun sequence.
ACCESSION   DACEXQ010000336 DACEXQ010000000
VERSION     DACEXQ010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1471
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.348"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            16..>1471
                     /gene="acm"
                     /locus_tag="IXH04_002786"
     CDS             16..>1471
                     /gene="acm"
                     /locus_tag="IXH04_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAQ1853321.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRLADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWED"
BASE COUNT          561 a          229 c          291 g          390 t
ORIGIN      
        1 ggggagatag aaaaaatgaa aaagtgtaga aatattattt tatcaatgtt atttattatt
       61 actaatataa catcactgat tcctgttcat gtctatgcgg atgcaggcag agatatcagc
      121 agtaacgtca cttcgttgac cgtggaccca acgaatatta cagatggagg aaacataaaa
      181 gtaaaattct cgtttgacga aaaaaaacaa aatatccaac caggtgatta tctttggatc
      241 aattggccaa gtgaaggaaa tattcgcggt gaaggattcc aaaaagaaat cccattaatg
      301 attgaaaata aaaatgttgg aactttaacg gtcagaaaag attctgcgca ggttgtgttt
      361 aatgaaaata ttaaaaacct agactcagta gaaggttggg ggcaatttga gatacaggcc
      421 agaaacgtaa ccgataccgg tgaagaaaat acgggaccat tcaccgtgac gagcggtgat
      481 aaaacagcta ctgtaaatgt gacaaagcca gcttctggtt cttcttctag cgttttttat
      541 tataaaacag gagacatgct acctgaagat actaagcaca ttcgatggtt tttgaatatt
      601 aacaataacg gcacttatgt tgaacaacca gttaaaatca gtgatgaaat ccaaagtgga
      661 caaagattag atccaagtac gtttgaaatt aatcaaatac acttaggtga acagaaggtt
      721 tatagaggag aagagggaat ccaacaattt ttacaagatt tccccagcgc tacatttaat
      781 ttttcagtaa ctgataatta cattgaaata actataccaa aaaactttgt caatcttagg
      841 aaaataatgg tgagttataa gacgatcata gaaaacccag aacaaataaa ttttgagaat
      901 cattcggaag cttggtttaa agaatttaat aaacctgctg ttgatggaga aagtttcaat
      961 catacagtta aaaatatttc agcttctggt ggagtcaacg gtacagttag aggcgaattg
     1021 aagattttca aatatatcaa tgacacagag attggtatcc caaatgtgac tttcgaatta
     1081 agactagcag atgaacagcc tattcaagga cagtctagta tcctgttgac ttcgaatgaa
     1141 cagggagaag ctagtatcaa aggcctgcaa gtaggagact atgttgtaaa agaaaaagaa
     1201 gcacccaatt ggattgattt tgatccacta agttcaaatg aactcaaatt ctccataaac
     1261 gaaaatgata cagagggagt cagtttacct atttataaca agaaaaaggt tacgaacatc
     1321 acagcaacta aaatttggaa cggtggaacc actccaagac cgagtattta ttttaaatta
     1381 ttcagagcaa gtacaaacaa ttgggaacca gttcctgatg cagaaacaaa acgattagac
     1441 aatggaataa cttcagtgac ttgggaagat a
//
LOCUS       DACEXQ010000337         1468 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.349, whole genome shotgun sequence.
ACCESSION   DACEXQ010000337 DACEXQ010000000
VERSION     DACEXQ010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.349"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..518)
                     /locus_tag="IXH04_002787"
     CDS             complement(<1..518)
                     /locus_tag="IXH04_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAQ1853322.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKE"
     gene            complement(642..1304)
                     /locus_tag="IXH04_002788"
     CDS             complement(642..1304)
                     /locus_tag="IXH04_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAQ1853323.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
BASE COUNT          382 a          284 c          273 g          529 t
ORIGIN      
        1 ttttctttat ctaatttcac tgaaatcttg tcaatttgtg caccaatctt ttgatatttt
       61 gctgtcactt catagatcga ttgtttgacc ttcccgaaga cacgctggaa gatgttccct
      121 tctccggcac gcagttcgtc aggattggct tcttggagac gatacatcag ttcagttaag
      181 gaatctccta caggaccaat atcttgtgct tgcacatgat tcaacatcga ttgtgagaat
      241 tcacttaatt ttgcttgggc agccgatccg tagctgatga cagactgtgc atcattagca
      301 tcgatttttg ctgctagctg tttagcttgt tcttggcgtt ccgccggtag tttgtcaatt
      361 aaacgagcag ctgtctgttg ctcttggagc tgatcaatct ccgcttgttg tgaagtggtc
      421 agcgtatctg ttggagttga aaacgggttg ttcaataaat cttcaagtgt atcgttaact
      481 ggtgtaacat cttttgtttc agtcggttta tgatccataa aaaattcctc cttgttgaat
      541 ctgttctcga ttcttacatg aatgtctcta ctttagaaaa agtactcgaa acagccagac
      601 atcatctgaa tgttccgagt cttcttttat gaacaaaaga gttaatcctt ttgttctggt
      661 tcatatttat tatcacgctt taagctctgt ttcgcaacag atatctctac gtcgatatca
      721 tctagatcct cagcaacgaa tttttgataa tcttgtgcga ttaaagctga cagctggtcg
      781 atgatctgcg cgctttcttc tagtttttca taggtctgtt tgtttttgat ctcatggtca
      841 ttgatctcat tatacttatt tgtcaaatca actagattcg gcagatgcgt ataaagaaac
      901 tgacttgctt catgcagtcg gtttggttct ttcaccaatt ctttgaataa agctttcgct
      961 gctttgactg gatcatggcg gagatcgatt gctttaagtt tggctgtctg ctgcatattc
     1021 atttgcaatt gattgatctc attttttgtt tgattcattg tttgtcgaaa aaatgaaatt
     1081 tcattcgctg tcataccgct tttttcatag tgagtttcaa gttcgtcagt cagtgcagga
     1141 atttcttctt gttttccctt tttcctgcgc gaaccaaaga gtccccacaa aactaaaact
     1201 acacctatca ataaaagtcc tagaacaaaa gaaaaactat tgtttcggac aaagagaaat
     1261 agcaagacca atgctagaag ccacggccaa tttcgtttta tcataataaa ccctccatgt
     1321 catttatcgt tgagtattga attcttttcg ttattcgatt acatacttat tttagcatac
     1381 ggactactat aaagagtatc ggactaaaga aggattttct atatttagag aaaatctaag
     1441 gtttatctat ggtaaactaa taaggagt
//
LOCUS       DACEXQ010000338         1455 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.350, whole genome shotgun sequence.
ACCESSION   DACEXQ010000338 DACEXQ010000000
VERSION     DACEXQ010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.350"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(43..1032)
                     /locus_tag="IXH04_002789"
     CDS             complement(43..1032)
                     /locus_tag="IXH04_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1853324.1"
                     /translation="MNQNLEKQTKLSIIIPFYNAKNTLKRAVDSVLTQGYPNIEILLI
                     DDGSTDGSSDIGDEIANLESKVACYHLENAGPSVARNCGIKEATGNLVGFLDSDDYFL
                     PDCFNNVLKDYHRNYPDIIAFGLSKGKNIENANLFVPEKKIGCSSEEAIEQMFRSKAV
                     DFYVWNKFFKRELFNHIVFPENKLYEDMVPMYEAFRAASKIDILPFSGIFYYQNNDSI
                     VYQSFNSKQYDNILQRKILVDEVKKDYPRLINLANARLVDGYLSTGFKITGKNQNQKE
                     KQKYYNQSKREIKSIYSKIRSNNEISIAKKLALFLYLINPKTYFILYKKVLKK"
     gene            complement(1004..>1455)
                     /locus_tag="IXH04_002790"
     CDS             complement(1004..>1455)
                     /locus_tag="IXH04_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ammonia monooxygenase"
                     /protein_id="HAQ1853325.1"
                     /translation="EFTGTLSNVIGRMIVPEYNDEIRVFSKRRNPFNHMTVMFDKNAI
                     LEVGNYRPLIGFEDYYLWVRLLKAGYKGYNIQEILVHARAGADMYARRGGSKYLLPGI
                     KGRYRVWKDGLGSLKDFLIVICGHLVISLLPNKIRGKVYESKLRKTN"
BASE COUNT          482 a          269 c          165 g          539 t
ORIGIN      
        1 ccgaagttaa taaacataaa aaataacata tcgaaaaaaa ctttattttt ttagaacttt
       61 tttatataaa ataaaataag ttttaggatt aattaagtat aaaaataaag ctaatttttt
      121 agcaattgat atctcattgt tacttcttat ttttgaatat atacttttta tttctctttt
      181 agactgatta taatattttt gcttttcttt ctgattttga ttcttgccag taattttaaa
      241 ccctgttgaa aggtatccgt ctactaatcg agcattggcc aaattaataa gtcgaggata
      301 atctttcttt acttcatcaa ctaatatttt tctttgcaaa atattatcat actgttttga
      361 attaaaagac tgataaacga tgctatcatt attttgataa taaaaaatac ctgaaaatgg
      421 caaaatatca attttactag ccgctctaaa agcttcatac ataggaacca tatcctcata
      481 taatttattt tctggaaaaa ctatatgatt gaataattcc cttttaaaaa atttattcca
      541 tacgtaaaaa tccactgcct tacttctaaa catttgctca attgcttctt cacttgaaca
      601 accaatcttc ttttcgggaa caaataaatt tgcattttct atattttttc ctttacttaa
      661 tccaaaagca atgatatcgg ggtaattcct atggtagtct ttcaaaacat tattaaaaca
      721 atcaggtaaa aaataatcgt cactatctaa aaatcctacc aaatttccag ttgcttcttt
      781 tatcccacaa ttcctagcaa ctgatggacc agcgttttcc aagtgataac atgctacttt
      841 actctctaga ttagcaattt cgtcaccaat gtcactacta ccatctgtag atccatcatc
      901 aattaacaag atttcaatat tgggataacc ttgcgttaag acgctgtcta cagctctttt
      961 taaagtattt tttgcattgt aaaaaggtat gattatagaa agtttagttt gtttttctaa
     1021 gttttgattc atatactttt cccctaattt tattcggtaa taaactaatt acaagatgtc
     1081 cgcaaataac tatcagaaaa tcttttagtg atcctagacc atctttccat acgcgatatc
     1141 ggcctttaat tcctggtaaa agatatttac ttcctccacg tctagcatac atgtcagcac
     1201 ctgctctagc atggactaga atctcttgga tattataccc tttatatccc gccttcaaca
     1261 gtcttaccca caaataataa tcttcaaatc caattaatgg gcgatagttt cccacttcta
     1321 aaatagcatt tttatcaaac atgactgtca tatgattaaa gggatttctt cttttcgaaa
     1381 acacacggat ttcatcatta tattcaggta caatcattct tccgataaca ttgctaagcg
     1441 tgccagtaaa ttcct
//
LOCUS       DACEXQ010000339         1427 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.351, whole genome shotgun sequence.
ACCESSION   DACEXQ010000339 DACEXQ010000000
VERSION     DACEXQ010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.351"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(138..509)
                     /locus_tag="IXH04_002791"
     CDS             complement(138..509)
                     /locus_tag="IXH04_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853326.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(511..912)
                     /locus_tag="IXH04_002792"
     CDS             complement(511..912)
                     /locus_tag="IXH04_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853327.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            complement(926..1333)
                     /locus_tag="IXH04_002793"
     CDS             complement(926..1333)
                     /locus_tag="IXH04_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853328.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
BASE COUNT          451 a          252 c          188 g          536 t
ORIGIN      
        1 ttgtttgata tcgtgactga cgtgccggac gcacgtagcg atcatcggcg ttatgactat
       61 ataaagtata ctttcgttga tccagaaaga tacaaagatt aaataaaaaa ataacaaaca
      121 aactcgcttg aaattcatta ggtgagtttg tttattattt tttctgcttc tcgattcatc
      181 agcgccatat ctgttttcgt aatggtttca ttaggaatat agtcagcaca ttttcttttc
      241 tctaacattc tatacccact attatgtatt cctaaggtat tcaattcctt aactgttgaa
      301 aacaaattcc aaacggtttg gtggcttcct tttccgctac ttccattata aataatatca
      361 taattttctt tcaaatactc atcagatttt ttaaaagcac tgtagtatgc tcttgaaaca
      421 gcagttcttc tataagattc tttagaatta tatttatcat ctgattcaat ttgtttggaa
      481 atagataaat attcattaaa gtcaaacatt ttattccccc aaagttgcta ctacagattt
      541 ccccatatat ttatagtcag ataatacatt atcgagagta tccaaaacat cgtaatctga
      601 ggaaaacaat tcagatgttg ttttgataac aaagaaatac aagtttaaat cttcatcata
      661 ttttatattg atttcagctt ttctttttaa aaccgtattt atcacatcaa aaattttgcc
      721 atcattactt gaaattgtgt taatctcatt ttttgtagta attacaagtt gattatcatt
      781 tttagttttc ggagtagaac ttgcatccac aacaactgga gatgctagaa ttaccgatgc
      841 gcaagcaaaa atcgcagcaa aatttattaa gctaccactc cgatgtgtat ttgatggtac
      901 tatatcaagc atccgtatcg tctccttaat ttttttccac tggtttgttg accttgatat
      961 caaagatttg ccttccaatc tcagttccat taaatttaac aacagcataa tgctctcctg
     1021 cttcttcaac caagacattt ctggcgttta tattaaagtt tatatcgtta acttgaggag
     1081 gcaagcttac cgatacttca cccgtagaaa aaatttgttt ttcattcttg tttaaaactt
     1141 caatttctac tatttttatc ttttctgtat cctctatctc agacaccaca gctgttacag
     1201 aaaatgataa agcagtagga attaaaggcg cattaataga aagagctgga ttaattactt
     1261 tattaataac attagtgcca aattgctgac tttctacatc ttctgaaatt attaattgta
     1321 ctctataaga cattgatttc tcctaatttt tacatttatt taataaattt atgtttcact
     1381 tttgctagtc aatttagttt aacaatagta acaaagcttt gtcaacg
//
LOCUS       DACEXQ010000340         1424 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.352, whole genome shotgun sequence.
ACCESSION   DACEXQ010000340 DACEXQ010000000
VERSION     DACEXQ010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.352"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(150..1337)
                     /locus_tag="IXH04_002794"
     CDS             complement(150..1337)
                     /locus_tag="IXH04_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAQ1853329.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          414 a          309 c          249 g          452 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact ttta
//
LOCUS       DACEXQ010000341         1403 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.353, whole genome shotgun sequence.
ACCESSION   DACEXQ010000341 DACEXQ010000000
VERSION     DACEXQ010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.353"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            168..362
                     /locus_tag="IXH04_002795"
     CDS             168..362
                     /locus_tag="IXH04_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853330.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            631..930
                     /locus_tag="IXH04_002796"
     CDS             631..930
                     /locus_tag="IXH04_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853331.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1027..>1403)
                     /locus_tag="IXH04_002797"
     CDS             complement(1027..>1403)
                     /locus_tag="IXH04_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1853332.1"
                     /translation="TNYTKDTARLARAFEQSESLIKRNGEGKIIGIVRWITDFATIAF
                     IQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELLTDDTEKTKKFYESVGFVQVK
                     EMGGISYVIDTRRKKVLFGNDK"
BASE COUNT          520 a          208 c          219 g          456 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta
      601 taatgtatca gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa
      661 ctacttaagc aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa
      721 gtgacagagt atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca
      781 aaaggaatat acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct
      841 ttaaaaaaaa atcatgtgtt cattttttat attagttcga atttgcaaaa aattgtattt
      901 gataaagagg tgagtaaaaa aataaaatag ctgagcattg gaggatagat tgagtgatac
      961 taagtctatc ctcttgtttt tctaaagaac taagctagaa ttttttcttt tgtacgttat
     1021 tatatgttat ttgtcattac caaaaagcac cttttttctt ctagtatcta tgacataaga
     1081 aataccaccc atttccttta cttgaacaaa tcctactgat tcataaaatt tttttgtttt
     1141 ctcagtatca tctgtcaata attcgatttg gactggtcca taactagtga tcttctccaa
     1201 agcttcattc aaaagcactt tgcctattcc ttggcgctga tacctcggat gaatgaggat
     1261 atcttgaata aatgcgatcg tcgcaaaatc agtgatccaa cgaacgattc cgataatctt
     1321 cccttcccca ttacgcttta tcagagattc agattgttca aatgctctag ctaaccttgc
     1381 agtgtctttt gtgtaattcg tcc
//
LOCUS       DACEXQ010000342         1380 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.355, whole genome shotgun sequence.
ACCESSION   DACEXQ010000342 DACEXQ010000000
VERSION     DACEXQ010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.355"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..620)
                     /locus_tag="IXH04_002798"
     CDS             complement(<1..620)
                     /locus_tag="IXH04_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853333.1"
                     /translation="MLEVMIIMNFDFDLKSFIDIFVPVLTSFAVCFITLFFTNKQHKK
                     AMKQQERQHEESLDLSKQQYADEKQRNEEQDRLSYYPYLKLIPKISEHRFAGKMVKSD
                     ENDVFKLPFELINEGLGMAFSIDFVYLEETINTNDHEVNLLSLLSAQKHIDDSLDALG
                     VSAPIDTDILRVGKKTEFELYWMKYNNEVMSIPKYSVSKWEIKIRF"
     gene            complement(669..1172)
                     /locus_tag="IXH04_002799"
     CDS             complement(669..1172)
                     /locus_tag="IXH04_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ1853334.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFSPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPIGEVALEIQHAFFNSFEEMVEH
                     VDDIICYPDCNDMSDVAYYLIEETGAFGEVPTHLQNYIDYEAYGRDLELEGNYLVTNR
                     GTFEFIG"
     gene            complement(1184..1345)
                     /locus_tag="IXH04_002800"
     CDS             complement(1184..1345)
                     /locus_tag="IXH04_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002392227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853335.1"
                     /translation="MAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGVKDVLL
                     NLFNRIVGA"
BASE COUNT          444 a          299 c          186 g          451 t
ORIGIN      
        1 ctaaaccgta tttttatttc ccatttactc actgaatatt tagggatact cattacttcg
       61 ttattatatt tcatccaata taattcaaat tctgtttttt tccctactct tagaatatct
      121 gtatcaatag gcgctgaaac tcctaacgca tccagactat catcaatatg tttttgagcc
      181 gaaagtaatg ataatagatt tacttcatgg tcgtttgtgt tgatagtttc ttctaaatac
      241 acaaagtcaa ttgaaaaagc cattcccaga ccttcattaa taagctcaaa tggtaattta
      301 aaaacatcat tttcgtcaga ctttaccatt ttaccagcaa aacggtgttc agatattttg
      361 ggtataagtt ttaaataagg gtaataactt agacggtcct gctcttcatt tctttgtttt
      421 tcatcagcat attgttgctt tgataaatca agtgattcct catgttgtct ctcttgctgt
      481 ttcatcgctt ttttatgttg tttatttgta aaaaaaagtg tgataaaaca tactgcaaat
      541 gaagttagta cagggacaaa tatgtctata aatgatttaa ggtcaaagtc aaaattcata
      601 ataatcataa cctccagcat tatatattta tcaataatta tatccctaaa cctatatcat
      661 gaaaatgttt atcctatgaa ttcaaatgtt cctctatttg tcacaagata atttccttct
      721 agttccaagt cacgtccata tgcttcatag tcaatatagt tttgcaagtg tgtaggcacc
      781 tctccaaaag cacctgtttc ttcaatcaaa taatacgcca catcactcat atcgttacaa
      841 tcgggataac agataatatc atccacatgc tccaccattt cctcaaaaga gttgaagaaa
      901 gcatgttgga tttccaaagc aacttcacca atcggtgttc cttctagttc ctctgctaaa
      961 ccacacaacc gattgatttc ctcaattggt gtatattcat caatctcaaa aggcaactca
     1021 taatcatgga tcgcatactc ttcgtattcg tcatttaaac caatccgttc tttcacttcg
     1081 tcaaaatcga caggtgggct aaaccaagcc cccaccaatt caccttcgtt atattttcca
     1141 aggttcgcaa tatatacacg catctgctcc ataatggtcc cccttacgca ccgacaatcc
     1201 gattaaacaa gttgagtaag acatctttca caccagaagc gttgaagacc agtcctaccg
     1261 caatcagcgc aatcaccaaa aaaccaatca gcttggaaaa ctcacgtttg aagcctaaaa
     1321 ataaaccaat gaccacaatt gccaataaga ctaaagattg ggcattggtt aaaaaccatt
//
LOCUS       DACEXQ010000343         1380 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.356, whole genome shotgun sequence.
ACCESSION   DACEXQ010000343 DACEXQ010000000
VERSION     DACEXQ010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.356"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            6..512
                     /locus_tag="IXH04_002801"
     CDS             6..512
                     /locus_tag="IXH04_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853336.1"
                     /translation="MDIKWLVQQNDSNLELAIKYLEETIFEDEHLTDNFLQVLKYLEI
                     YSVKKNKLIGENDSPIKTPIELSLRNRMGILQRSEIVKELFYHKFSYEIRLDDTYEHY
                     RIVFFVYNSIEDATATTALTFGFTKNGTINSDKTRQAATESDDICKKVCNGEENYWIG
                     EEKLNEIY"
     gene            499..951
                     /locus_tag="IXH04_002802"
     CDS             499..951
                     /locus_tag="IXH04_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853337.1"
                     /translation="MKSINDLLKKHPDLYKKLNSEQRNLSKQLLKIRLKKQKNVTEFS
                     KMLGISEKKYLEFEFGDMHIPVAEYNKLLKQVENLSYKDSMNFTIKVAGGPIKYDRIE
                     NKPYIRNKSYSVFKSRKRKEVSSNMVYASVNYQDTSRYIVEKEVYVHG"
     gene            944..1342
                     /locus_tag="IXH04_002803"
     CDS             944..1342
                     /locus_tag="IXH04_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853338.1"
                     /translation="MDKGNSVTFALENISLNKIHFELTDNFSYGDLEFNIELGYKEDE
                     AEYLEKKLVSLRAHIVGGMEGAPVDILLFSVIRVSTNEQPYKKLVEESMGELAKPILN
                     KASNLISNLYSEVGVMPTVIDLYQLYSQQK"
BASE COUNT          552 a          160 c          240 g          428 t
ORIGIN      
        1 gtgagatgga cattaagtgg ttggttcaac aaaatgatag caatttagaa ttggcgatta
       61 aatatctcga agaaactatc tttgaagatg aacatcttac agataatttt ttacaagtat
      121 taaaatatct tgaaatttac tctgttaaaa aaaataaatt aattggtgaa aatgattctc
      181 caataaaaac acctattgag ctaagcttaa gaaatcggat gggaatactc caaagatcgg
      241 aaatagtaaa agaacttttt tatcataaat ttagttatga aataagacta gatgatactt
      301 atgagcatta tagaattgtt tttttcgtat acaattctat agaagatgct actgcgacta
      361 ctgcattaac attcggtttt acgaaaaatg gaacaatcaa ttcggataaa acccgtcaag
      421 ctgctacaga aagtgatgat atatgtaaga aagtatgcaa tggggaagag aattactgga
      481 taggggagga aaagttgaat gaaatctatt aatgatttat taaaaaaaca tcctgattta
      541 tataaaaaat taaattctga acaaagaaac ctgtccaaac aattacttaa aataagatta
      601 aaaaaacaaa aaaacgtgac agaattctca aaaatgttag gaataagtga aaaaaaatat
      661 cttgaatttg agttcggaga tatgcatatt cctgttgcag agtacaataa acttttgaaa
      721 caagttgaaa atttgtccta taaagattca atgaatttca ctattaaggt tgctggcggc
      781 cctattaaat atgaccgtat tgaaaataaa ccttatattc ggaataaaag ttactcagta
      841 tttaaaagta gaaagagaaa agaagtatcg tcaaatatgg tctatgctag tgttaattac
      901 caagatacga gccgctatat tgtagaaaaa gaggtatatg tacatggata aaggaaatag
      961 tgttactttt gcacttgaaa atattagttt gaataaaatt cattttgaat taactgataa
     1021 ttttagttat ggagatttag agtttaatat tgaacttgga tataaagaag acgaagcaga
     1081 gtatctagaa aaaaaacttg tttctttacg agctcacatt gtagggggga tggaaggagc
     1141 accggtagat attttactct tttcagttat tcgtgtttct acaaatgaac agccatataa
     1201 aaaattagta gaagaatcta tgggagagct agctaaacct atcctgaata aagcttctaa
     1261 tttgatttct aatttatata gcgaagtagg agttatgcct acagttattg atttatatca
     1321 gctatatagt caacaaaaat aaaatacaag taatagagtt atctatttaa atttgcaaga
//
LOCUS       DACEXQ010000344         1374 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.357, whole genome shotgun sequence.
ACCESSION   DACEXQ010000344 DACEXQ010000000
VERSION     DACEXQ010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.357"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(190..1125)
                     /gene="whiA"
                     /locus_tag="IXH04_002804"
     CDS             complement(190..1125)
                     /gene="whiA"
                     /locus_tag="IXH04_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAQ1853339.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(1159..>1374)
                     /locus_tag="IXH04_002805"
     CDS             complement(1159..>1374)
                     /locus_tag="IXH04_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853340.1"
                     /translation="LVNTEKVPEDYMDPEIYDEYLVQVAHDFNGLRNEGCRVISTDFL
                     ELKDGGVFHDGDKVVEELFRLVFGSKY"
BASE COUNT          393 a          302 c          220 g          459 t
ORIGIN      
        1 actaaaatct tcattcagtt gtctccccgc tttttatacg attgtattaa aaaacaatag
       61 aagcctttca acattatgtt actccagtaa agttggaatt atagtttgac ggaagaaaag
      121 caagtatagc ttttaaaaaa aactgataca ttcgcatctc ctgcttcatg tatcagtttt
      181 ttctttgaat taaactgctt cttcacgcag tttttccgca aattcattta ttttgcgaat
      241 tcggtgatta attcccgatt ttgatattgc acctgaggga atcatttccc ctaattcttt
      301 caagctgacc tctggatgtt gaagccgaag ttctgcgatt tcctgaagtt tttcaggaag
      361 ggcatgtaga ccgacctttg cttcgatcaa ttcgatgttt tcgatttgtt tagaagccgc
      421 atcaatcgtc ttgttcagat ttgctgtctc acaattgact agacgattga ccgaatttct
      481 catatcccgt acgattcgaa catcttcaaa tcggagcatt gagttggttg caccaatcaa
      541 tgttaagaaa tcagcaattt tttctgctcc ttttaaatac gaaatatagc cattgcgccg
      601 ttcaagcgtt cgagcatgta gaccgtaata atttaacata tcgcaaatgt cttggttatg
      661 ctgttcataa atagaaaata tttccaaatg ataacgactc gtttccggat tattgactga
      721 acctgaggcc ataaaagcac cacgtaagta agaacgcatt ttttgcgaat ttcccataat
      781 ctcgtccgaa acgtgtccat tgaacatcat accatccatg atatcaagat ccatcaacac
      841 atcttttgtg ccttgcttca accggacgat atagacattg ttctttttta atttcatttt
      901 tttacgaacg agtagctcac ttcgtacctg ataatgatct ttcaaaagag aatagatacg
      961 tctggcaatt gcagcatttt ctgtttggac gttcaaaata aactgctggt tcacaatacc
     1021 taaagagccg ttcattcgca gcaaagcagc taattccgct ttcgcatgtt ctcgatgaac
     1081 ttccaaacca gttagttctt tcttcacatc tgcagcaaat gacatcctat tctcctcctt
     1141 tctccaacaa taaattgctt aatattttga accgaaaact aaacggaaca attcttctac
     1201 tactttgtct ccatcgtgga agacaccacc atctttcaat tctaaaaagt cggtggaaat
     1261 cactcgacat ccttcatttc tcaatccgtt gaagtcgtgt gcgacttgta ctagatactc
     1321 atcatagatc tctgggtcca tataatcttc aggcactttt tccgtattga ccaa
//
LOCUS       DACEXQ010000345         1368 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.358, whole genome shotgun sequence.
ACCESSION   DACEXQ010000345 DACEXQ010000000
VERSION     DACEXQ010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.358"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            82..>1368
                     /locus_tag="IXH04_002806"
     CDS             82..>1368
                     /locus_tag="IXH04_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAQ1853341.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQLIN"
BASE COUNT          467 a          263 c          257 g          381 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taatcaat
//
LOCUS       DACEXQ010000346         1368 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.359, whole genome shotgun sequence.
ACCESSION   DACEXQ010000346 DACEXQ010000000
VERSION     DACEXQ010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.359"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(9..473)
                     /locus_tag="IXH04_002807"
     CDS             complement(9..473)
                     /locus_tag="IXH04_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853342.1"
                     /translation="MAAKRIKPNTNLKETQNHTAGIPRDAATLNYHSLGKQNADQEHP
                     CLNLKYYDFNFECFSELSQEDLKSFTALNRKFRDLTWLQIKQQSGKTNKSGLAPTKIP
                     RNKLPKSPILDAISEDIEILELRLSKKARVFGFRSAATFFLIFLDSQHRICP"
     gene            complement(479..946)
                     /locus_tag="IXH04_002808"
     CDS             complement(479..946)
                     /locus_tag="IXH04_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SocA family protein"
                     /protein_id="HAQ1853343.1"
                     /translation="MHKPKDVAKWFVANTDVASGSQITPLKIQKLLYYAQAWYLAIYN
                     EPLMDTEFEAWAHGPVVPEVYHGLSDFKYNPVSISAEYFSDAEEVSCEQEVDLLQQIM
                     DIYGIYDGKYLEKLTHQEDPWIQTRGDLPPEARCNEVISREVMKDFYRNMQGE"
     gene            complement(1222..>1368)
                     /locus_tag="IXH04_002809"
     CDS             complement(1222..>1368)
                     /locus_tag="IXH04_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1853344.1"
                     /translation="YHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLN
                     IPKR"
BASE COUNT          411 a          256 c          194 g          507 t
ORIGIN      
        1 ttcaaaaatt atgggcatat tctatgctgt gagtctaaaa aaattagaaa aaaagtagct
       61 gctgatctaa acccaaaaac tcttgctttt ttagataatc ttagctccaa tatttcaata
      121 tcttcgctga ttgcatctaa aataggactt ttcggcaatt tattccttgg aattttcgtt
      181 ggagctaatc cagatttatt tgtctttcca ctttgttgtt taatctgtag ccatgtcaaa
      241 tctctgaatt ttctatttaa agcagtaaaa ctttttaaat cttcttgtga caactcagaa
      301 aagcattcga agttaaaatc ataatacttt aaattcaaac aagggtgttc ttgatcagca
      361 ttttgttttc ctaaactatg ataatttaaa gttgctgcat ctcttggaat gccagcagtg
      421 tgattttgcg tttcttttaa attcgtgtta ggttttatcc tttttgctgc cacaattatt
      481 attccccttg catattacgg tagaaatcct tcataacttc tctactaata acctcattac
      541 atcttgcttc aggtggaagg tcacctcttg tttgaatcca cggatcctct tgatgagtca
      601 acttttcaag atactttcca tcgtatattc catatatatc cattatttgt tgcagtaaat
      661 ctacttcttg ctcacagctt acctcttctg catcagaaaa atactctgca ctaatactca
      721 cagggttata cttaaaatcg gatagcccgt gatatacttc cggtacaacg ggaccatggg
      781 cccatgcttc gaattcagtg tccatcaagg gctcattata tattgctaaa taccaagctt
      841 gggcatagta caacaatttt tgaattttta atggggtgat ttgcgaacct gaagctacat
      901 ctgtatttgc tacaaaccat ttagctacat cttttggttt atgcacgctt atccctccta
      961 tttttgttta cttctactat acaaattatc cgatacctat aatataaagt caatatatat
     1021 aagttaattt attagctaat tttgttaaca attttgtttg tatatcggat agtatataaa
     1081 tattaaaatt gcttgtatat aagaaacaag ctatattttt actatctaaa tagtatgcag
     1141 ttttactaac aaattagcta acaatattag caactatatc aacaactata tgtacttccc
     1201 ctcaaaaatg aggggctatt tttatcgttt aggaatattc aagtaccagc gtttatcatg
     1261 aaaatcttgt gctccgcctt tagtgttccc ttctggatca ttcgttgccc gcatcattac
     1321 atagactttc ttactaggaa aattacgcat attgaaagat acatgata
//
LOCUS       DACEXQ010000347         1365 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.360, whole genome shotgun sequence.
ACCESSION   DACEXQ010000347 DACEXQ010000000
VERSION     DACEXQ010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.360"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..650
                     /locus_tag="IXH04_002810"
     CDS             <1..650
                     /locus_tag="IXH04_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HAQ1853345.1"
                     /translation="REYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPN
                     VSIVSSDNFEGGKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFK
                     IPNNLSTIRREMEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYD
                     GTSFIEKFFPQLTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(820..>1365)
                     /locus_tag="IXH04_002811"
     CDS             complement(820..>1365)
                     /locus_tag="IXH04_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ1853346.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IKQKLNWQSPVQFRKTTVTVV"
BASE COUNT          449 a          230 c          224 g          462 t
ORIGIN      
        1 agagagaata cattgaaatg cttgaagcca accaagttga tggcataatc tcatcaagcc
       61 ataatcttgg aattgctgat tatgagcgtg tgagagcacc tattgttgct tttgatagaa
      121 atttagctcc taatgtttcc attgtttctt cagataactt tgaaggggga aaacttgcag
      181 ctaagacttt acaaaaaaat ggttgtcaaa ataccattat gattacagga aatgataaca
      241 cggattctcc cacagaatta cgtgcactag gtttttcttt tcaaaatcca aatggaaaaa
      301 ttttcaaaat tcctaataat ctgtctacta tccgtcgtga aatggaaata aagtcaacta
      361 ttataaatca caaaccagat ggtatttttg tttctgatga tttaacagcc attctcacta
      421 tgaaaattgc tcatcaactt aaacttaata tcccagaaga tttaaaaatt attggatacg
      481 atggtacctc ttttattgag aaatttttcc cacaattgac aacaataaga caaccaattg
      541 atgaaattgc aagtcttatt gttgatattc ttctgaaaaa aattaaaggc gaaaagacaa
      601 ataaggatta tattttacca atctcaatac tttctggagg aagtatttaa cgttaaatta
      661 attttttaat gtctataaaa ttcgttagaa aatacaattt gtagatatcc aattgatttg
      721 taaacaaaaa ggagttgtca acttgtagtg acccccaaaa gttagacttt tttggagtgg
      781 agaaatccac tccttttttc atgctctaaa atgatatttt tacacaactg ttacggttgt
      841 tttacgaaat tggacgggac tttgccagtt taatttctgt tttatccgtt cattattgta
      901 gtaatagata tagctatcga tggctgattt taattcctcg aaacagttat aaactttccc
      961 gtgaaaaatc tcttgtttta ataagccaaa aaaattctcc ataagtgaat tatctaagca
     1021 gttccctttc cgagacatgc tttgaaagat tttgtactcc ttcaatttgt ttctataagc
     1081 tttcatttga tacgcccaac cttgatctga gtgaaacgta cgacgatagg gacaatcgtt
     1141 agtggtctgg atagcttcct ccaaaccttc cattaccgct aatgcattgg gcttttcaga
     1201 gatacgatag gataaaatct cactattgaa catatccata aaaggatcta gatacaattt
     1261 cttttgacgg ataatgcctt tggcatccac ttcatggtat ttaaattcag ttgtatccgt
     1321 cgtgattttc tgatggcaga tactggtgta gaaacgacga tggat
//
LOCUS       DACEXQ010000348         1352 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.361, whole genome shotgun sequence.
ACCESSION   DACEXQ010000348 DACEXQ010000000
VERSION     DACEXQ010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.361"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            11..1350
                     /locus_tag="IXH04_002812"
     CDS             join(11..452,452..1350)
                     /locus_tag="IXH04_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAQ1853347.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT          565 a          175 c          235 g          377 t
ORIGIN      
        1 ggtgcttttt ttgcggaaat atacatttgt ttttaagaaa aaagtagttt cagactattt
       61 aaataacgaa ggcggctaca aatatcttgc acataaatat caaataaatc gtacactggt
      121 tagacattgg gtaaggattt ataactatca tggttgggaa ggcttggttg gaggtggcaa
      181 aagctacact acaaaattta aacttgatgt tatagaatat atggaaacaa atggtctttc
      241 tatccaagaa actgctaaaa aatttaatat cggttcaaat agaactctaa gtaaatggat
      301 agagcaatat gaagaaggcg gtgcttcttc acttgagagc caaaaaaggg gcagaaaaat
      361 tagtatgaat tccaagctaa acattcctaa aaaacttaaa gatgagtctc ttgaagaaga
      421 agttattcgt ttaagagcag agaacgcata tttaaaaaag ttagaaacct tgattcaaga
      481 acaagatttg agcaagaaga aatcaaggtt aaaacaatcc ttcagctcaa aaaagaattt
      541 aaattgaatc tattactttc aattgcacaa ttagcgaagt caacttatta ttactgggta
      601 aaaaaattag ataagccaga taaatatagc aagataaagc aagaaattac agcgattgta
      661 aaagaatcaa gaaactctta tggttatcgt agagtcactt tagcgttaaa gatgaaagga
      721 tatacaatca atcataaaac agttagaaaa ttaatgtccc aaatgggact tacctgtcaa
      781 atccgaataa agcgatataa atcttataaa ggaacagtag gaaaaattgc caagaatgtg
      841 ttaaaacgaa atttttcagt agatacaccc aataaaaaat gggtgacaga cgtcactgaa
      901 ttcaagataa aaggaagaaa aatctatcta tctcctatac ttgatttatt taacggagaa
      961 ataattagtt atagtatatc aactagtcca acatataaac tgattgaaga aatgcttcaa
     1021 caagcaatta aaaaaaaggg aactgagggt tcactgattc tacattcaga tcaaggatgg
     1081 cagtatcaga tgccacaata tcaaaaaaaa ttaaaagaga ataacattat tcaaagcatg
     1141 tctagaaaag gaaattgctt agataattct gtaatagaaa atttttttgg tgtgcttaaa
     1201 tcagagtttt tttatcgaga aaaattccga tcaattgaga tatttcaaag taaattaaat
     1261 gagtatatta ggtggtataa taacaaaagg ataaaactaa agttaaatgg actatctcca
     1321 gtagaatatc ggaaacagtc cattaaatag tt
//
LOCUS       DACEXQ010000349         1292 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.362, whole genome shotgun sequence.
ACCESSION   DACEXQ010000349 DACEXQ010000000
VERSION     DACEXQ010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.362"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            415..1263
                     /locus_tag="IXH04_002813"
     CDS             415..1263
                     /locus_tag="IXH04_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAQ1853348.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
BASE COUNT          419 a          244 c          229 g          400 t
ORIGIN      
        1 ttagattgat tagtgaaaat aaattctgtg cattttttga taatagtttt tgccatctca
       61 tcgatcattt ctcacctgat ttctaaacat attgtttgat caaaatgggt tcactcctaa
      121 aaagataaaa aactcccttt ctatttattt aaatacgcaa atcttcctca ttatttttta
      181 ttttactcaa tttaatcgta ttctcatttc aaaacgaatg tactttaaaa aaacataata
      241 agatcacttt gcttgtagga tgtattccct tccattacaa tgaaataaat agcgatttta
      301 tgagaaggtg atgtgaaaat ggaagaaaaa atagaagaat ccaaacaaaa aatcgatcag
      361 ttgatataag aattagaaaa agcggtcatc ggcaaacgtt caactatcca attgatgatc
      421 gtggccctat tagcaggtgg tcatgtctta ttcgaagata ttcccggtgt aggtaaaacg
      481 ttactggtca aagcactagc aaaagctgta cagggaacct tcagcagagt ccaatgtact
      541 cctgatctgc tgcctagtga tattcttggt ttttccgtat acaattcaca atcaaaagag
      601 tttgaatttc gtccgggacc gatctttacc acgatcttgt tagcagatga gatcaatcgt
      661 acgactcctc gtactcagtc agcactttta gaagcaatgg ccgagaatca tgcaacgatc
      721 gacaacaata cataccctct agacaatcat ttttttgtct tagctacgca aaatccaatc
      781 gagtacgaag gaacatatcc tttgccggaa gcacagctcg atcgtttttt attccgacta
      841 caaatcggtt atccttcttt tgatgacgaa cttttgctac ttttggataa attcgaaaaa
      901 gtgttggata acttaaatga agtattgaac agttatgaaa tagaagaaat aaaaacaaat
      961 gtggcgaatg tctttgtcag tcccgaagtg gcatcttatg ccttgcagtt agtgtcagca
     1021 acaagaagtc atgctgccat ccagcttggc atcagtccac gaggaagcct ttcctttatt
     1081 caggcagcaa aagcttatgc tttaatccat gggagaaatt acgtcacgcc caaagatttg
     1141 caggatttag ttccttacgt gttttcccat cgcctgattt tttacgatcg ttcattgaag
     1201 gaagacgaaa agaaacaaat cattcatact cttgttgcac aaattcctat tcctgtgagg
     1261 tagattgagg tagacaatgg aaaaaataaa ag
//
LOCUS       DACEXQ010000350         1289 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.363, whole genome shotgun sequence.
ACCESSION   DACEXQ010000350 DACEXQ010000000
VERSION     DACEXQ010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.363"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..675)
                     /locus_tag="IXH04_002814"
     CDS             complement(<1..675)
                     /locus_tag="IXH04_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ1853349.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEF"
BASE COUNT          399 a          229 c          229 g          432 t
ORIGIN      
        1 gaattctttt ggaggtgttc ctaatgagat agctaagaag ttataaatgc tttctagcat
       61 ttcttctttc ttttcttcaa tagcgtctaa tgtttctcct tgagcaacca ttttccgtaa
      121 cactactgca tctttacgca gtaatttgtt caagtagtta ttcaaatctc gagaatttga
      181 actattcgca ctttccggca tcaccgattt aggcacaacg ccatattttt ggaataatga
      241 aacgatcata tcccattggc cgccatcttg ttgaggagta gcgagtaaga aagctacttt
      301 ccggctatcg attgcctctt ttcctgttgc aatgatgttt tcatagaaat aatttgcctt
      361 ttcatattta tcccagaaaa aagtgtaatt ttgtgataat tcaaactctt ttaaatggaa
      421 ttgattcagc atcttatgac ggaatgtgtt cagtgcagca aacatccagc aacgccccga
      481 ttgcatttga ttagccactt tccccgtagc caaatctata gaaaataccg gtgtattctc
      541 cacatgtgct gaaacatttt cagcagaagc agtaattccg ttcttcacaa ctgcccgctg
      601 caatgcctgc tgtttgatat ttgcttgaaa ctgtttgtta aattttgttg tcatatcagg
      661 cgtaatagct ttcatccaat catccttcca aatccaagtt atacttcgta ttttactctt
      721 aagaatttag aaatacaatc tcttctatga tgaaattttc atttgaatga gaatatactt
      781 acaacttcat gaagaaaaat gtcatacggt atacaatagt tttcagataa aaaggaatat
      841 ttctatgaga taacgaaaat ggcctgtata ataaagaaga ggcctagaag aacatcatac
      901 aataagatag ctttcaaaat actcattgaa agaaggaaat actataacgt aagaaaggag
      961 atgaataaaa tgaaaaacct gattgctggc ggctttttgt ttgtgggtgg tggcatactt
     1021 atgaatacag ataactttgg gaatgtcatc ccttttcttg gtgcagcttg tgctttgatt
     1081 ggtgtaggaa tgctgattta tttcgtattt agcgatgatg ggaagtatgg ttgattatga
     1141 gtagccaact ctactctttc gtaaagatta caacatacgt gacaaaaaca gaaagcttga
     1201 ttcatcataa taataaaaga aaaagaaacc caagtgattg gatttctttt tctttgcata
     1261 agctttattt actccggcag taggactcg
//
LOCUS       DACEXQ010000351         1267 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.366, whole genome shotgun sequence.
ACCESSION   DACEXQ010000351 DACEXQ010000000
VERSION     DACEXQ010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1267
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.366"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..1160
                     /locus_tag="IXH04_002815"
     CDS             join(<1..274,274..1160)
                     /locus_tag="IXH04_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ1853350.1"
                     /translation="SKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKWV
                     KQSKTSGSFKEKDNLTPEQKELLELRKRNQQLEMENDILKQAALIFGPKRQVIDANKH
                     LYPISAMCRILGLSRQSYYYQSKPKKDESELEEVVAEEFIRSRKAYGSRKIKKALSKR
                     GIQISRRKISRIMKNRGLKSSYTVAYFKVHHSTCNEAKTTNVLNRKFLRDNPLEAIVT
                     DLTYVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLVKKAFSRIKQPLTEVEIFHT
                     DRGKEFDNQAIDELLTTFDINRSLSHKGCPFDNAVAESTYKSLKVEFVYQYTFETLQQ
                     LDLELFDYVNWWNHLRLHGTLGYETPVGYRNQRLAQRILDNELGCANASEAV"
BASE COUNT          453 a          229 c          251 g          334 t
ORIGIN      
        1 tctaagagaa cacgaagaac tttttcacaa gaattcaagc aacaaatcgt caatctttac
       61 ttagctggaa agccacgtgt agaaatcatt cgagaatatg aactaacggc ttcagcattt
      121 gacaaatggg taaagcaatc taaaacgagt ggttcattca aagaaaaaga taatcttacg
      181 cctgaacaaa aagaattgtt agaactacgt aaaagaaacc agcaattaga aatggaaaat
      241 gatattttaa agcaagcagc gctgatattc ggacgaagag acaagtaatc gatgcgaata
      301 agcatcttta ccctatatca gcgatgtgca gaatattagg tctatcacgt cagtcctatt
      361 attatcaatc aaaaccaaag aaagacgaat cagaacttga agaagtagtc gctgaagaat
      421 ttatccgcag ccgaaaggcc tacggctcaa gaaaaataaa aaaagcctta tcaaaacgag
      481 gcattcagat cagccgacga aaaattagta gaatcatgaa aaatagagga ttaaaatcga
      541 gctatactgt tgcttatttt aaagtacatc attctacttg caatgaagcc aaaacgacaa
      601 acgtattgaa tcgtaaattc ttaagagaca acccattaga agcgatcgta acagacttga
      661 cttatgtacg agtcgggaaa aaatggaatt atgtctgttt cattttggat ctgttcaatc
      721 gagaaattct cggctattct tgtggagaac ataaagatgc cgttctagta aaaaaagcat
      781 ttagccgtat caaacaacct ctgacagagg ttgagatttt tcatactgat cgtggaaaag
      841 agtttgataa ccaagctatt gatgaattat taacaacttt tgacatcaat cgatcattga
      901 gtcataaagg ctgtcctttt gataatgccg tagctgaatc aacttataag tcgttgaaag
      961 tagaatttgt ctatcaatac acatttgaaa ccttacaaca attggatttg gagttatttg
     1021 actatgtcaa ttggtggaac caccttcggt tgcacggtac acttggctac gagacaccgg
     1081 ttggttaccg taaccagaga ttggcgcagc gaatccttga taatgagctc ggatgtgcta
     1141 acgctagcga ggcagtctaa ctttaactgt tagctcctgc cgaagatcgt cacatccgag
     1201 gaggctcatt gtcaaggaca atcggaatag catacggaaa gaagttctgc accttataaa
     1261 atttgtc
//
LOCUS       DACEXQ010000352         1261 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.367, whole genome shotgun sequence.
ACCESSION   DACEXQ010000352 DACEXQ010000000
VERSION     DACEXQ010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.367"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..1225)
                     /locus_tag="IXH04_002816"
     CDS             complement(<1..1225)
                     /locus_tag="IXH04_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1853351.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYY"
BASE COUNT          461 a          274 c          209 g          317 t
ORIGIN      
        1 gataatagat aataaatgct atcaatgtgt tgatcatgat caagataaca acacgccagt
       61 catttcctgc tgaaagataa ccgccgattg gagctggtaa tgtccatgtt ggcaaaacag
      121 taaatctatt gaccatttct aatttgactg caaaataact gataatagtc aaaactaaag
      181 gaactaagtt gaacgggatg aacatgtaag gattcaacat cataggagct ccaaaaatta
      241 tgggctcatt gatattgaag ataccaggaa taatagaaaa acgaccaacc tctttcaaga
      301 attttgattt acagaagaat ataaacaaaa cagataccac gatcgttgct cctgcaccac
      361 caattgttac cgtccattga taaaactgtt caggagcgat atagggtaat tgggtggcag
      421 ctgtaccatc tgccaatgcc tttgcattct catccaacat gatcagccac attggacgaa
      481 caacagcccc tacaatactc atgccatgga taccaaatga ccacaacaaa tggatcaaaa
      541 acattggtag taaaacacca agcaaattat tccctaaaat attggttaaa ggctcgaata
      601 acgataataa ggcagcgttt acatcaaagc ccgcgacgct tcgaatcagc caagtaattg
      661 ttaaaatgac tgcacctgga atcaatgcct caaaactacg ggcaacagca ggtggcactt
      721 gttctggcat tttgatagta atatttcgat ttttaaagaa acgatatact tccactgcaa
      781 aaatcataga taagatcccc gcaaacatcc cagatgctcc caaagaactc ataggtaaga
      841 cccagccaag aggtgcctct tcaggagtta aaacgtttac aggaacttgt aacataaaga
      901 acgttcctaa agataatata ccacctgtca cactatctaa tccatatgat ttagctagat
      961 ccgaacccat aacaaaagtt gcataaatag ccatcaatcc catcgtcatc ctaaagggta
     1021 aaacaatcgt tgccgaataa ggggcaataa attcattcca tccaggaaac ggtaaattga
     1081 taaagaccat aaaaaatgtc ccaatcaaaa tcaatgacaa tgttgaaaca accccattac
     1141 gaattgctaa taaatgacgc tgtgtaccaa tttttgccat tggtgggaca agtttctctt
     1201 cgacccattt catcagcttt tccactaaaa ccactccttt tcaataaaat atgaaccatt
     1261 c
//
LOCUS       DACEXQ010000353         1246 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.368, whole genome shotgun sequence.
ACCESSION   DACEXQ010000353 DACEXQ010000000
VERSION     DACEXQ010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.368"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            200..1246
                     /locus_tag="IXH04_002817"
                     /pseudo
     CDS             200..1246
                     /locus_tag="IXH04_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180785.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
BASE COUNT          514 a          141 c          237 g          354 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tcttgatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DACEXQ010000354         1237 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.370, whole genome shotgun sequence.
ACCESSION   DACEXQ010000354 DACEXQ010000000
VERSION     DACEXQ010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.370"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(390..>1237)
                     /locus_tag="IXH04_002818"
     CDS             complement(390..>1237)
                     /locus_tag="IXH04_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HAQ1853352.1"
                     /translation="ITRYKALKYIEDAREKQLVSISINSPYARNYELEEIFKNYFKTN
                     IYILKSSEDLSQHDLLFSKFAADYIQELIKKANVVALSWGDTVYKVIDQFKMGAREEL
                     IFTQFIGEIGKYNSLAGSTRLVQKAAERYESSYLTLSIPLYVINETARELMALEPSLA
                     NTLATASHADMLISGIGTLSSIDSVEPWNKHRKILFPSMTDTVGFLYGRPFNINGEFI
                     KASEDKTFGLSLKEIFDIPKRIGICKSKFKAESCLGALNGNFFTDLFLDEKTAWKILS
                     LIKKE"
BASE COUNT          455 a          197 c          205 g          380 t
ORIGIN      
        1 cgtggagaaa taataagaac taccagtcag ataaaacaag ataccgaaga ctcactgcgc
       61 aaagggtggg gaatgaataa taaagttaga tagaaccccc tttttatagc ataaaaaaga
      121 tttagaagga tatatgatcc tattgcatga gaagtacaat aaaattaata agaagtaaca
      181 ttaacgaaaa aacgtggaaa ttattcagtg aatacaaaga aattaaatct tgaaaagtaa
      241 tggagtatgg cttggaaaag ttcaaaaaat atcaagacaa gcttctcaaa aaaagggatt
      301 ttgatatcca ataaaaagag tgaatcattg tactgccctc caaaaaagta gtaccaaaaa
      361 tctaaatttt ttggaggatt ttacatgact cactcttttt ttatcaaaga aagtattttc
      421 caagcagttt tttcatccaa aaataaatct gtaaaaaaat taccattcaa ggcgcctaaa
      481 caactctccg ctttgaactt acttttacaa ataccaattc gttttggaat atcgaaaatt
      541 tcttttaaag aaagaccaaa agtcttatcc tcagatgctt tgatgaattc accattgata
      601 ttgaagggtc ttccgtaaag aaatccaaca gtatccgtca tgcttggaaa taggatttta
      661 cgatgcttgt tccagggttc gacagaatca atagaggata aggtgccaat tccagaaatt
      721 aacatatctg catgcgaagc ggttgctaag gtattagcta aagaaggttc tagagccatt
      781 agttcacgtg cagtttcatt gatgacatat aaaggaatac tgagagtcaa ataagaagac
      841 tcataacgtt cagcagcttt ttgaactaat cgtgtggagc cagctaagga attatatttt
      901 ccgatttcgc caatgaattg agtaaaaatt aattcttcac gagcacccat tttaaactga
      961 tcaatcactt tgtaaacggt atctccccaa gatagtgcaa ccacattggc tttcttaatt
     1021 aattcttgaa tgtaatcggc agcaaatttt gaaaataata aatcgtgttg tgataagtct
     1081 tcactacttt ttaatatata gatattcgtt ttaaagtaat ttttgaagat ttcttctagc
     1141 tcataattcc tagcatacgg agaattaatt gaaatactaa ctaattgttt ttcacgtgca
     1201 tcttcaatat attttaaagc tttatatctg gtgatcc
//
LOCUS       DACEXQ010000355         1223 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.371, whole genome shotgun sequence.
ACCESSION   DACEXQ010000355 DACEXQ010000000
VERSION     DACEXQ010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1223
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.371"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            170..>1223
                     /locus_tag="IXH04_002819"
     CDS             170..>1223
                     /locus_tag="IXH04_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853353.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHC"
BASE COUNT          379 a          206 c          184 g          454 t
ORIGIN      
        1 aataacgata aatttttttc aaattatagc tgtaaaaatg tttcccttta ataattatat
       61 gtttctaatt ctatttcctc atctttatct cattttttat tataaatagt tgacgttagt
      121 ttaaaaaacg tttaccctaa cattacaagt tttagaaagg aataccttta tgtctttttt
      181 taataacata tataataaaa aagaatcttt ttatttacgt ataaagtgga gcataatcat
      241 tatattatca ttgttatttt taacaggaat tgcttatttc ttttatttta ttttttctcc
      301 tgcggacagc aatcaaatga aagaaataaa agattcgtgg gttttctata caaaagatga
      361 tcctgatttt aagtttgaca gcaagtacac tcgtacgatt cctaccgtgg ataaagacga
      421 aacattcatt atggaaacga cacttgaaaa accgttggac gaagcaaatt tactaatcaa
      481 aggaaaccat caatggatta ctgtatatct aggcgaaaag attttatacc aacggaaaga
      541 ttatcaggaa gaaagcaatc ccggactctc attggctgtg atagatttac cagcaaatta
      601 tatcggaaaa aaattgaccc ttacagtctc atctccttat cagaactatg ctggactgcc
      661 accgaaagtt tatctaggaa caactggacc attgattagt tttatttatt cacaatcagt
      721 gccgcaagta ttgacgatga taatcgctgt ttttatcgct atagggacct ttttctatac
      781 gatctacttg atgtataaac aaaaacgtct tgaatactca cttattctct taagttgctt
      841 tgctttggca ttaggatttg aagctgtatc agaagatatt ttaagtggta ttctttttga
      901 accattcgtt cattcttttt tatctcattt atttattatc ttaacgtcag ctttcatcat
      961 ctgctactat tggagccgaa tgttttacta taaaaaatgg tacggaattt tttgtacgtt
     1021 ccaacttttg atttttatcg gcgttctgct ttatgcaact tttactcctg cagaacttcc
     1081 tgaattaatg ccgattgcta atattgctag tgttataagc actttagtca cttcacttgc
     1141 atgcattggc gaagcatata agaataaccg tttttatgta gcttgtacac cttggatcgt
     1201 cttggtagcc attatccatt gct
//
LOCUS       DACEXQ010000356         1220 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.372, whole genome shotgun sequence.
ACCESSION   DACEXQ010000356 DACEXQ010000000
VERSION     DACEXQ010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.372"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(234..>1220)
                     /gene="cls"
                     /locus_tag="IXH04_002820"
     CDS             complement(234..>1220)
                     /gene="cls"
                     /locus_tag="IXH04_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ1853354.1"
                     /translation="VLHVLEEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHT
                     FVTSQRALLRFRLNYHDHRKIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIF
                     GAASSLLQLRFLTDWNVSVPEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNE
                     REQIKLSFIKLITSAKKRVWIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPF
                     IYRATQYYARQLIKENVQILVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETS
                     AVIYDPEFLEELATQFKEDETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
BASE COUNT          361 a          227 c          245 g          387 t
ORIGIN      
        1 ccgcagatgc aagcttttga acaccgttta agaaagaggt cgtgagcctg ccgtccagct
       61 ccgagtcaca aggaacaagt tcaaaaaaca gcttctcatg tttgttgaat attgtgactt
      121 gtgaccgagg agttggcagg agaacaccgt ttgttcggaa gaagggactg tgacaagatt
      181 tttgtcacag tccctttaat cttagtatat ttttctctac ttgaataaaa aaattatagg
      241 attggagaaa ataatcgaga aatttgttgc ttgaataata gccagttaga catttccttg
      301 actgtttcag ttgtcatgga tgaacaatgt gtctcatctt ctttgaactg agtagcaagt
      361 tcttctaaaa actcaggatc gtaaatgaca gcacttgttt caaaattcaa tcggtagcta
      421 cgaatatctt gatttgctga acctaccatg cagatttcat catccattat caatgttttt
      481 gcatggagga agccgttctc atagacaagg atttgtacat tttccttgat cagctgccga
      541 gcataatatt gtgttgctcg ataaataaaa ggatgatccg gtttgttggg aatcatgatt
      601 ttcacatcta caccagaggc agttgcgatt tttaaagcag caatgacact ttcatcagga
      661 acaaggtaag gtgtctgtat ccaaacacgt tttttagcag aagtaatcaa tttgataaat
      721 gaaagcttga tttgttccct ttcgttattc ggtccgcttg aaacaagctg gatggatgta
      781 tcagcaagct tagattcatc tcgatctgct tttttaaaga aataattcaa atgatagccg
      841 accttttttt cttcaggtac cgagacgttc cagtctgtta agaagcgaag ctggagcaaa
      901 gaagaagcgg caccgaaaat ccgtatatgc gtatcgcgcc aatagccgaa tttttttgtt
      961 gtatttacat attgattggc aatattgaaa ccaccggtat aactaatctt tccatcgatc
     1021 acaacgattt ttcggtgatc atggtaattc aatcggaaac gaagtaatgc cctttgtgag
     1081 gtaacaaatg tatggacatg tccaccattt ttgattagac gattgaaatc ttttgctttt
     1141 gtgccatgag agccaaatgc atcatataat attcgaactt ccacgccttc tgcggctttt
     1201 tcttctaaaa catgtaagac
//
LOCUS       DACEXQ010000357         1218 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.373, whole genome shotgun sequence.
ACCESSION   DACEXQ010000357 DACEXQ010000000
VERSION     DACEXQ010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.373"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            235..>1218
                     /locus_tag="IXH04_002821"
     CDS             235..>1218
                     /locus_tag="IXH04_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HAQ1853355.1"
                     /translation="MLDEVLEIHKKNVGVLRMETTEAVQDDQDLAKMYTPGVAELSLM
                     IEKNHALARELTISGKLVAVITDGSAVLGLGNVGNQAGLPIVEGKALLYKNLAGVNAI
                     PLAIEQKSVDEIVQTIVNLQNSFAGIHLEDIAAPKCFEIEEKLQEKLSIPVYHDDQEG
                     TAIVVLAGLINAAKIQRKTLTELRVLINGMGASGVATARLLIAAGIKNLTLVDKQGRL
                     KKQDSTLNKYQRELLDHIHDTNNNASVLEDAIVGQDVFIGLSIGNVLTPEMIGKMNDN
                     PIIFALANPVPEITPDLAKESGAKLIATGSSKYPNQVNNILVFPGLFKGLLL"
BASE COUNT          444 a          167 c          242 g          365 t
ORIGIN      
        1 caaattttta caaggagtga ttcatagtca gagaattcca gatgcatagt atagctccta
       61 actttttcaa atagatattt tttctcatta taccaaaaaa attgaattta gtcttttttt
      121 aaatttttaa aaataagcat cattttttta aaatttaaaa tgaaagggtt tttttatttt
      181 aaaaaaagtg ttaaagtggt tttgtaatta aaaaatgtaa aaaggagtga aggtatgctt
      241 gatgaagttt tagaaatcca taaaaaaaat gtaggtgttt tacggatgga aacaacagaa
      301 gccgtgcaag atgatcaaga tttagcaaaa atgtacaccc caggtgtagc agaactaagt
      361 ttaatgattg agaaaaatca tgctttggca agagaattaa caattagtgg taaattagtg
      421 gccgttatta cagacgggtc ggcggtatta ggtcttggaa atgtaggaaa ccaagctgga
      481 ttaccaatcg tcgaagggaa agcgctgctt tataaaaatt tagcaggtgt caatgcgatt
      541 ccattagcca tcgaacaaaa atcagtagac gagattgtac aaacaattgt caatttgcaa
      601 aatagttttg cagggatcca tttagaagat attgcagcac cgaagtgttt tgagattgaa
      661 gaaaaattac aagaaaaact ttctattcct gtgtatcatg acgatcaaga aggaacagcg
      721 atagttgttt tagctggatt gatcaatgct gctaagatcc aaaggaaaac attaactgaa
      781 cttcgagttt tgatcaacgg catgggagca tctggggttg caacagctag actattaatc
      841 gctgctggta tcaaaaatct aacattggtg gataaacaag ggagattaaa gaagcaagat
      901 agtacattaa ataaatatca aagagagttg ttggatcata tacatgatac aaataataat
      961 gcaagcgttt tggaagatgc aatcgttgga caagatgttt ttatcggtct ttcaatagga
     1021 aatgttttaa cgcctgaaat gattggaaaa atgaatgata atccgattat ttttgcttta
     1081 gccaatccag taccagagat cacacccgat ttagcaaaag aatctggtgc aaagctaatc
     1141 gctacaggtt cttcgaaata tcctaaccaa gtaaacaata ttttagtatt tcccggattg
     1201 ttcaagggac tattacta
//
LOCUS       DACEXQ010000358         1212 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.374, whole genome shotgun sequence.
ACCESSION   DACEXQ010000358 DACEXQ010000000
VERSION     DACEXQ010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.374"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            28..1095
                     /locus_tag="IXH04_002822"
     CDS             28..1095
                     /locus_tag="IXH04_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ1853356.1"
                     /translation="MQRVLMICNDSSVKGGITSIITQMLNFNWEKKNIEMTFIPSYKG
                     GNAIFKSLYFMYAFAKIIGISLLSKPNIIHMHMSYRGSFSRKYVLNKWFRLLNIPVII
                     HLHGSEFKKWYDSVSVKKQNQIRLLLRESDSTIVLGEKWKKIIKEIEPNCKTVVVENS
                     IVVPEYTIKWNSKMCRFLFMGVLIKRKGVHDLVEAVKNIAEQYPEKKFIFEIAGAGDE
                     EISLKELIEEYNLQSYFEFTGWVVGEKKEECYRRSNVMILPSYNEGLPISILEAISYG
                     MPVIATDVGDISSAVRDGENGYLFQPGDVSALTSAILKINDKDKYVKFSRCSKDLAIT
                     KFNDGNFFDRILNLYQKGSKE"
BASE COUNT          455 a          127 c          249 g          381 t
ORIGIN      
        1 aaatataaat ataaggagta aagcgttatg caaagagtac ttatgatttg taatgacagc
       61 tcggtaaaag gtggaattac aagtataatt acacaaatgc tcaattttaa ttgggaaaaa
      121 aagaatatcg agatgacttt tataccgagt tataagggtg gaaatgcaat atttaaatct
      181 ttatatttta tgtatgcatt tgctaaaatt ataggtatct ctttattgag taaacctaat
      241 attattcata tgcacatgtc gtacagaggg agttttagta ggaaatatgt gctgaataag
      301 tggtttagat tattaaatat tcctgtaatc atccatcttc atggatcgga atttaaaaaa
      361 tggtatgata gtgtttctgt aaaaaaacaa aatcagattc gcttgctgtt aagagagtct
      421 gattccacga ttgtacttgg agaaaaatgg aagaagataa ttaaagaaat tgagccaaat
      481 tgtaaaactg ttgttgtaga aaattctatt gtagttccag aatataccat aaagtggaat
      541 tcaaaaatgt gccgatttct gtttatggga gttttgataa agcgaaaagg tgtacacgat
      601 ttggtagaag cagttaaaaa cattgcagaa caatatccag aaaaaaagtt tatttttgag
      661 attgctggag caggtgatga agaaatatcg ctcaaagaat tgatagaaga atataatttg
      721 caatcgtatt ttgaatttac aggttgggtt gtgggagaaa aaaaggagga gtgctataga
      781 agatcaaatg tgatgatttt accgtcctat aacgagggat tgcctatatc tattctagag
      841 gcgatttctt atggaatgcc tgtaattgct acagatgtag gagatatttc ttcagcagta
      901 cgtgatggag aaaatggata tttgtttcaa cctggtgacg tatcagcgtt gacatctgca
      961 atactaaaaa tcaatgataa agataaatac gtaaaatttt cacgttgttc gaaggatttg
     1021 gcaataacga aatttaacga tggaaatttc tttgatagaa ttttaaatct gtatcaaaaa
     1081 gggagcaagg agtgatgaca aggaaaataa aaaatgtttc gattatgtaa tgaacttatt
     1141 aagtgaataa aatagagaga aaaaatattg aaatagacga tgtaaatttt tatccgtgta
     1201 agaattcaaa tg
//
LOCUS       DACEXQ010000359         1210 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.375, whole genome shotgun sequence.
ACCESSION   DACEXQ010000359 DACEXQ010000000
VERSION     DACEXQ010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.375"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            2..247
                     /locus_tag="IXH04_002823"
     CDS             2..247
                     /locus_tag="IXH04_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853357.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            244..663
                     /locus_tag="IXH04_002824"
     CDS             244..663
                     /locus_tag="IXH04_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAQ1853358.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            660..>1210
                     /locus_tag="IXH04_002825"
     CDS             660..>1210
                     /locus_tag="IXH04_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAQ1853359.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVV"
BASE COUNT          439 a          171 c          277 g          323 t
ORIGIN      
        1 aatgataaaa aaactcgttc aattcagcat ggatttatat gatatcgaat caggagcaac
       61 actatctgtg gaatcggacc atctaatcat aaattttggt ggaaagcgcc agattatttt
      121 gtgggtagtt gatgatgtac tgtttccaga aattgttcat gatttcgaag aatcaaaagc
      181 ggttgagttt gaaatagtga aaaaagtaat ggaattgatt gaaaaatacg aggaggacag
      241 cgaatgatac cgaagtttag agcgtgggat aaacgaaaga acgtaatgag agatgtagcc
      301 gtcttgcatt ttactaaaaa cggcaaaaca aactttattg aatattggat aaatcctacc
      361 gaattgaaat catatcacgt gcgaaacatc gacctcatgc aatccacagg aatgaaagat
      421 aagaatggtg tggatatatt tgaaggggat atagtattag tcagcgtgcg aaatggcttc
      481 gattacttag ataataaagt ctgtattgtc aaaaattcaa tagattattc cggattagtt
      541 tgtgccactg ttgatgaaga cttagagtat caaattttta acacagagct gtttgaagaa
      601 tacacgtacg aagtcaccgg aaatatatac gagaatagcg agttattgga ggaacagcga
      661 tgaataaaca ggaagcaatc aaaaagttag aaagtattaa agcgatagga aatgatgcaa
      721 tagctgcttg ctataacgag agtataaatt caggtattac gttaatgaaa aaaatagacg
      781 aaccgcagaa accagttatt ccacaactcg tggccggttg gcttgagaaa tctacggacc
      841 cttttacaaa agctgaaaaa atagcgtatt taatcaaatc taaagatggt gattcatatt
      901 atttctgtga ttggtttgta cgagatggca tagtgacgca agagcaagga gaagaattac
      961 tcgcttgggc aacaagacaa tcatatgaaa cactattgag cctatacaac ggctacgagg
     1021 ttgagaaaga gccgttatgg gcaataaaga atgccgatgg aaactatctt actaaatgtg
     1081 ctttatgggg aaaagatgga gtaaattata gttttgaatg caatccatct catcgattgc
     1141 ttttcactga taaagcaaca gcggatgctg cagcattgtt ggtgaatgga acagtggaag
     1201 aggtggtaga
//
LOCUS       DACEXQ010000360         1203 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.376, whole genome shotgun sequence.
ACCESSION   DACEXQ010000360 DACEXQ010000000
VERSION     DACEXQ010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.376"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..158
                     /locus_tag="IXH04_002826"
     CDS             <1..158
                     /locus_tag="IXH04_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1853360.1"
                     /translation="RLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLERIIPNME
                     AYFSDYF"
     gene            178..672
                     /locus_tag="IXH04_002827"
     CDS             178..672
                     /locus_tag="IXH04_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAQ1853361.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            665..>1203
                     /locus_tag="IXH04_002828"
     CDS             665..>1203
                     /locus_tag="IXH04_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853362.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAA"
BASE COUNT          349 a          209 c          253 g          392 t
ORIGIN      
        1 ttcgactgga tatccaaaca ttgacttgct tgatgatgaa ttatcgccgt gcttcttatt
       61 tggcaagaat cgaacggtta gaaacagatg aagaaacact gaagtcacta gagcgtatca
      121 ttccaaacat ggaagcttac tttagcgatt atttttgata aaaaggatga tgtttagatg
      181 aaaatttatt ttgccgcacc aatgtttgct aaaagtgatt tgttatataa ccaacagtta
      241 gttaaagaaa tcagagagct ctcttctgag ctttcgatct atcttcctca agaaaatgaa
      301 gcaatcaatg acaaaactgc ttatgcagac agtcgaatga ttgctcttgc tgacacagaa
      361 aaagtgttgg ctagtgattt aatgattgct ttactagatg ggctgacgat cgatgccggg
      421 gtagcatcgg aaatcggtgt ggcttatgcg aaaggcatcc cagtgatcgg cttatatact
      481 gatactcgac agcaaggcgg aacccacccg aaaaaaatcg ctgcactaca agaaacagct
      541 gaaaatcaat ttcactacct gaacttatat acaataggtt tgatcaaatt gaacggaaaa
      601 gtggtttctt cagaaattga gttgctttca gaagtaaaaa gatttttaga tggagggact
      661 ttcagtgatt aaagaaatca aaaaggtgca aatagcttta cttgcttttg gtgtattcgt
      721 tgtctgctat aatttttatg aatttatcac acaaaaatat tcgacttctc aaggaatcac
      781 atttatcgtg gaatctttat tagggattgc attgattttc atgccacaag tcattttgaa
      841 agttttcaaa cttaaaatac cagcagcaat cgttttgttt tactggtttt tcttgttcat
      901 ctctgtcttt ttaggcacag ggatgcattt gatcagtatc atctcttttt gggacaaaat
      961 cttacatgcg gtgagtccaa tggtcctgac agctcttggc tatggattga ttggttactt
     1021 gatgaaagat gctgagatat caaaaaccag tccttggttg ttcctcttat tcggctttgc
     1081 gtttgctgga ctttgcggcg tgttttggga gttctgggaa ttcttatgcg accagttctt
     1141 aggcatgaat cttcaacgtt ttgctgcttc tgatggcaca ctgtttgtcg gacgagcagc
     1201 att
//
LOCUS       DACEXQ010000361         1195 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.377, whole genome shotgun sequence.
ACCESSION   DACEXQ010000361 DACEXQ010000000
VERSION     DACEXQ010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.377"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..1076
                     /locus_tag="IXH04_002829"
     CDS             <1..1076
                     /locus_tag="IXH04_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ1853363.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          211 g          332 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tcttg
//
LOCUS       DACEXQ010000362         1193 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.378, whole genome shotgun sequence.
ACCESSION   DACEXQ010000362 DACEXQ010000000
VERSION     DACEXQ010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.378"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..1180
                     /gene="pepV"
                     /locus_tag="IXH04_002830"
     CDS             <1..1180
                     /gene="pepV"
                     /locus_tag="IXH04_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAQ1853364.1"
                     /translation="TLGIFGHMDVVPAGDGWDTDPYEPVIKDGKIFARGSSDDKGPSM
                     AAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMAYYFEHEEEPDFGFSPDAEFPII
                     NGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVPGTATAALQVPSADAAIAMEEAF
                     YQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQSGINAGSFLALFLDNYEFLGSAK
                     QFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAGLFAFEENGTEEANFINMNFRFP
                     KGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVPMDDPLVATLLQVYEDHTGEKGY
                     EQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEFMSLDDLFRATAIYADAIYRLAK
                     "
BASE COUNT          391 a          216 c          282 g          304 t
ORIGIN      
        1 aacgctaggg atatttggac acatggatgt cgttcctgca ggagatggct gggacacaga
       61 tccttatgaa ccagtaatca aggatggaaa gatctttgca cgcggatcaa gcgatgacaa
      121 aggtccaagt atggcagctt actatgcgat gaagatcatt aaagaacttg acttgaaact
      181 atcaaaaaaa gttcgcttcg tagtcggcag cgatgaagaa agcggctggg gcgatatggc
      241 ttattatttc gaacatgaag aagaaccaga ttttggtttc tcaccagatg ctgaattccc
      301 aatcatcaat ggtgaaaaag gaaatgtttc tttagcttta cgcttcaaag gcgacaatgc
      361 aggagattat gtcttgaaat cttttgtttc cggacttcgc gaaaatatgg tgccaggaac
      421 agcaactgca gcacttcaag taccaagtgc agatgcagct atcgcgatgg aagaagcatt
      481 ttaccaattc atcgaagcaa atccagtaag cggaacgatt gaagcggaca atacgtatgt
      541 aaaaatcgaa ttagttggta aaggcgcaca tggtgcaagc ccacaatctg gtatcaatgc
      601 aggttctttc ttggcattgt tcttagataa ctatgaattc ctaggatctg ctaaacaatt
      661 tatccatgta gcagctgctt atgtccatga agatttttat ggagaaaaac taggagtcgc
      721 ttatgaagat gaaaaaatgg gcaaattgac aatgaatgct ggactatttg cttttgaaga
      781 aaatggtaca gaggaagcga acttcatcaa tatgaatttc cgttttccaa aaggtgtaac
      841 agtagacgga ttgcaaagtg acatcgaaca aacagtgggg caagaaggcg caacagttac
      901 tcgaggtgct cgcgtaatgg aaccacatta cgtaccaatg gatgatccat tggtggctac
      961 attattgcaa gtctatgaag accacactgg agaaaaaggc tacgaacaaa tcatcggtgg
     1021 cggaacttac ggacgtttgt tgaaacgtgg cgttgcttat ggagcgatgt tcccaggata
     1081 tactgataca atgcatcaag caaacgaatt tatgagtctt gatgacttgt tcagagcaac
     1141 agcaatctat gcagatgcca tctatcgttt ggcgaaataa aaatactaaa aaa
//
LOCUS       DACEXQ010000363         1190 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.379, whole genome shotgun sequence.
ACCESSION   DACEXQ010000363 DACEXQ010000000
VERSION     DACEXQ010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.379"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..715)
                     /locus_tag="IXH04_002831"
     CDS             complement(<1..715)
                     /locus_tag="IXH04_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ1853365.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFTLNDYVFSFEGLVVGTDIVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQ"
     gene            complement(766..>1190)
                     /locus_tag="IXH04_002832"
     CDS             complement(766..>1190)
                     /locus_tag="IXH04_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ1853366.1"
                     /translation="WIRDKISSVFDNLNISIPHIPLPHFKLSGEFNPLKGKIPTLGVD
                     WYAKGSVFNSPNIIGVGEAGPEAVLPLKRSVLQEIGDRILSSTSVSSRAQTIQPVNNY
                     EFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
BASE COUNT          368 a          237 c          191 g          394 t
ORIGIN      
        1 tttgtatttt gctcaaacca gtacaattaa aatgattaat atccacttgc aacgttggat
       61 attgcatggt cttcattttg ttggaccgat ctaaaatatc tccgtccgat tgctcttcgt
      121 agcaaagcat caaatcacta tcaatgacta tgtcagttcc cactactaag ccttcaaaac
      181 taaacacata atcatttaag gtgaattggc atgaagtagc tgaaggagtg atgataatct
      241 ttggaaaact ttcttctaaa ctattattca gcaagttaaa tgactgtggt ttatctacgg
      301 ttataggtac atcttcttga actcttgcga atggtttcgc agtgatattt acatcgaact
      361 ctccccattc aacaatatcg ttttctgcat ccccaatatc gatagtctgg ataacataat
      421 agacgttggg atcatcagag aattctaatt tctttgcata gtttaaccaa tgacgcatga
      481 tataaaacga ttgcttgaat gcttgatggt cttccacatc ttctaaatag ttatagtgca
      541 atgtaaacga catgtcttca aacgagtaat cttgtactaa gccacctagc cgtcctaaaa
      601 cagaagtttc aactctctgt ctttttggag aaggtatggt tggtctttcc gctaaagcca
      661 atttatgcaa ataatcagga aatccatcga ttatagaatg tatacaatca gtcatttttt
      721 cacatccttt ttaaaactaa aaaaacagga gaaatactct cctgtttaac gccatgccga
      781 agcattatca ttttgaactt ttgtaatgct atcaatgatt tgttgagttg tttgcttcat
      841 agtaacctca tctgcgttac catcaattgt gaaattgaat tcgtaattgt tcacaggttg
      901 aatcgtttgt gccctagatg aaactgaggt gctactcaag atacgatccc caatttcttg
      961 cagcacagat cttttcaaag gtaaaactgc ttcaggtcct gcttcaccga caccgataat
     1021 atttggagaa ttaaacacac tacctttcgc ataccaatca acacccaatg ttgggatttt
     1081 ccccttcaat ggattgaatt ctccgctcaa tttaaaatgt ggtaacggaa tatgtggtat
     1141 agaaatattc aaattatcaa agacactact gattttatct ctaatccaat
//
LOCUS       DACEXQ010000364         1182 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.380, whole genome shotgun sequence.
ACCESSION   DACEXQ010000364 DACEXQ010000000
VERSION     DACEXQ010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1182
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.380"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..119)
                     /locus_tag="IXH04_002833"
     CDS             complement(<1..119)
                     /locus_tag="IXH04_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
                     /protein_id="HAQ1853367.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVV"
     gene            569..>1182
                     /locus_tag="IXH04_002834"
     CDS             569..>1182
                     /locus_tag="IXH04_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1853368.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
BASE COUNT          423 a          207 c          198 g          354 t
ORIGIN      
        1 acaacaagtt ctgttgttaa tggatctaaa tttctttcta tacattcgac acttgcgtaa
       61 aagcttttca tatcgattaa aaagtaatca tttactggct cttttgaata atccatcata
      121 aataacactc cattctttca cattatacaa acatatgttc gatattgtaa agaagaaata
      181 gctaaataca taaaaaaaga agcataggta tgctatcacc ctgcttcttc ttttgatttg
      241 tatgatcaat ttattctatc actttactaa ttaatttgaa aatctaataa aaactctcac
      301 tctaaaagag tgagagactg caaattagag tacggacttt tttggagttc cgttctttaa
      361 tattaacaaa tgaataaaat ccttacaaca tttgagaata agttatcagt agacacttac
      421 aaaaaattct catactattt cctccatcat tagtttttgt ctatataagg tataccctaa
      481 ctagacacga ccgcgacatt gaaaaatctg gttttctcgt gtatgattag ggtgtttttt
      541 aaatagttgc agaaagtagg taaaaatttt gagagttgca tacatacgtg tctcatccat
      601 cgatcaaaat gaacaacggc agattgaaga aatgaaaaaa ttcggtgcag agagaatttt
      661 cattgagaaa caatctggcg caacgattac tcatcgacct gtttttcaag aagcactgga
      721 tttcgtccga gatcaagata tttttattgt ggaagctatt gatcgacttg gaagaaatta
      781 cgacgagatc atcgcttcag tcaattactt gaagaagaaa aatgtgcagc tgatcatcac
      841 aagtctaccg atcatggccg aagcaattgg caatcctctc ctggataaat tcattaaaga
      901 tttgatcatt caaattttgg ccatgatcgc tgaacaagaa cgtaccgaat ctaaacgacg
      961 tcaggcacaa ggcattaaga ttgcaaaagc gaacggtgtt tataaaggac gtcctaagct
     1021 atacagtgcc aatgcaaaag atccccaacg tcgtcttgtt tataaaaaca tcgttgagga
     1081 tctaaaaaaa ggtgttgcga tcgctaagat tgcaaaagac tacaatgtta cgagacaaac
     1141 cgtatataga ataaaaaaag acagcatggt caatcacgaa ta
//
LOCUS       DACEXQ010000365         1181 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.381, whole genome shotgun sequence.
ACCESSION   DACEXQ010000365 DACEXQ010000000
VERSION     DACEXQ010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.381"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            81..599
                     /locus_tag="IXH04_002835"
     CDS             81..599
                     /locus_tag="IXH04_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAQ1853369.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            706..>1181
                     /locus_tag="IXH04_002836"
     CDS             706..>1181
                     /locus_tag="IXH04_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ1853370.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEEL"
BASE COUNT          376 a          192 c          262 g          351 t
ORIGIN      
        1 aaaacaagat cagtcatcgg gctcaagcaa tgaaaaaatt gagtgccgag tggaaacaat
       61 ggctggaagg agagagatag atgcggtatt tagtagtaag tgataatcat ggagacagag
      121 atatcctcgt tgacttagta gatcgttatc gttctgaagt ggatatcttt ttccattgtg
      181 gtgattcgga attagaagct tctgacccat tatgggatac attccaagtg gttcaaggta
      241 attgtgatta tggaccagga ttcgaacaga aaaaagtgat ccaaactggt caggatacag
      301 tattcatgac tcacggtcat ttatcgaatg tccgatttgg tttgacacag ttagcaatcg
      361 aagctcgagc tgcaactgcc gatatggtgt tttttggtca tacccatcaa ataggctgtg
      421 agatggatca gaaaatcctg tttttgaatc ctggtagcat ttctcagcct agaggcccga
      481 tccaaatccc atcatatgct cttatcgaaa gtttagatga ccaaatttct gttcaatact
      541 ataaccgtgc ccatcaggca gttgaagagt tggccttttc tttttcgaga gagcgctgaa
      601 taaaaaaact ctaattttta ataaaaataa gctttatttt atgaattttg catccggttt
      661 cggtaaaata gtagtgttga atgaaataga aatggaggcc atcagatgat tggacctata
      721 gtaagagaaa tgctgctgga aaatcaggaa acgtttttga ttcctgcaga aaatgtggca
      781 aatgttatgt atcatcatcc attgtcccat ggtttacttg tgttatcgaa ggtcggttat
      841 tccaaaattc ctgtattagg aaaagatgat cactttgtag gactagtaag tttatcagat
      901 gtggtcaata aaatgattga cttgcaaaca atcagcatgg acccgttaga aggactaacg
      961 gttgctgatg tgacagaaac agatgttccg acaattgatg aaaattggga actagaagaa
     1021 gtactccatt tgttagtcga ttcgcctttt ttaccggttg tcacagaaga tcggattttc
     1081 aaaggaatca ttacaagaaa agagctgctt aaagccgtaa acttcatggt gcatgaatta
     1141 gaacgtcgta atatattgtc tccgaaaatc gaagaattaa a
//
LOCUS       DACEXQ010000366         1149 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.382, whole genome shotgun sequence.
ACCESSION   DACEXQ010000366 DACEXQ010000000
VERSION     DACEXQ010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.382"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(19..549)
                     /locus_tag="IXH04_002837"
     CDS             complement(19..549)
                     /locus_tag="IXH04_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HAQ1853371.1"
                     /translation="MRLGLIIKNILNLPKTIYFNYKFLHISDAWRLPILVHRKVRITR
                     KSRGKIEIVLQSGKIVYLGFPVLNTAGNEETIISIEGILRIQGPAFIAQGAHIDIRKN
                     AVLDIGSKFNSTGKMSIICFKEIQFKDGCTVSWDTLFMDTDSHTIFELNTHRILNKNN
                     KIVIGKHCWIGAKSVI"
     gene            complement(546..>1149)
                     /locus_tag="IXH04_002838"
     CDS             complement(546..>1149)
                     /locus_tag="IXH04_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ1853372.1"
                     /translation="AEIITDILYKLYEPFGFAVLLTILGLVYFMYATDGIAAGEGYKR
                     ILMELYLHLKKDRKYRKVCALIFYTAMILFTTLINRTIWLNPLSDVMGDWWIYKYNTT
                     TGTKELSTACLENIILFIPYVYMVMSIKYENIKKPKIWSSIVYGGKIAFYFSAIIEFL
                     QLFLRLGTFQISDFVYNTLGGILGVILYKLILITKRDGER"
BASE COUNT          392 a          217 c          127 g          413 t
ORIGIN      
        1 tgctaaatga acacctttct atatcacgct ttttgctcca atccaacaat gtttacctat
       61 aactatttta ttatttttat tcaaaatcct atgagtattc aattcaaaaa tcgtatgtga
      121 atcggtatcc ataaataatg tatcccacga tactgtacag ccatctttga attgaatttc
      181 cttaaagcaa attatactca tttttcctgt cgaattaaac tttgacccaa tatctaaaac
      241 tgcatttttc cttatgtcaa tatgagcccc ttgtgcgata aacgctggcc cttgtattct
      301 tagtattcct tcaatactta taatcgtttc ttcatttccc gcagtattta atactggaaa
      361 gcccaaataa actatttttc cagattgtaa tactatctca attttccctc tcgatttgcg
      421 tgtaatccgt actttacgat gcaccaaaat aggtaatctc cacgcatctg aaatatgaag
      481 aaatttataa ttgaagtata tcgttttagg taaatttaat atattcttta ttatcaatcc
      541 gagtctcatc tttcaccatc tctttttgta atcaaaatta gtttataaag gattacaccc
      601 aatataccgc ccaacgtatt atatacaaaa tccgaaattt gaaacgttcc tagcctcaaa
      661 aaaagttgta aaaattcaat tattgctgaa aaataaaatg caatttttcc tccatatact
      721 attgaggacc atattttggg tttctttata ttttcatatt taatagacat taccatataa
      781 acatatggaa taaaaagaat aatattttct aaacacgctg tagataactc ttttgttcca
      841 gttgttgtat tatacttata aatccaccag tctcccataa catcagataa aggatttaac
      901 caaattgttc gattgattaa tgttgtaaat aaaatcatcg cagtatagaa tatcaaagca
      961 caaacttttc tatattttct atccttcttt aaatgaagat aaagttccat tagtatacgt
     1021 ttatagccct cacctgcagc aatgccatct gttgcataca taaaatagac cagtcctaaa
     1081 attgtcaata aaacagcaaa cccaaagggt tcatataatt tatataatat atctgtaata
     1141 atttctgca
//
LOCUS       DACEXQ010000367         1148 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.383, whole genome shotgun sequence.
ACCESSION   DACEXQ010000367 DACEXQ010000000
VERSION     DACEXQ010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.383"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            79..234
                     /locus_tag="IXH04_002839"
                     /pseudo
     CDS             79..234
                     /locus_tag="IXH04_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
     gene            253..>1148
                     /locus_tag="IXH04_002840"
     CDS             253..>1148
                     /locus_tag="IXH04_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1853373.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGG"
BASE COUNT          481 a          100 c          199 g          368 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcagga taaggataag tgtattaatt gtggaaaatg taaaaaagtt
      121 tgccaaatga ataaagattc agttgagaat tgcaatcatc tggagtgtat cagatgtgga
      181 cgatgtaaaa atgcttgtcc agtggatgca attagttatg gagtaagaaa atagagagga
      241 gtagttagga aagtgataaa aaatgctata gcatatatta caagaaagaa aaatagaact
      301 ttaataattt ttatcatatt aactatagtt ctttcttgct tgtattcttg tttaacaata
      361 atgaaatcaa gcaatgaaat agaaaaggct ttatatgaaa gttctaattc ttcaatatca
      421 ataacaaaaa aagatggtaa atattttaat gttaatcaat ttaaagatat tgaaaaatta
      481 aaagaaattg aagaaaaaat aattcaatat gatggattag caaaattaaa agatgctaaa
      541 gtagttagcg gagaacaaag aataaataga gaagatttat ctgacgaatt taagaatgtt
      601 gtttcactcg aagctacaaa taatactaaa agaaatattt tatttagtag tggagtattt
      661 acaattaaag aaggtaaaaa tatagaagaa aatgataaga attcaattat tgttcatgaa
      721 gaatttgcta aacaaaacaa tctaaaatta ggtgatgaag ttgatcttga attactagat
      781 attgaaaaaa gtggaaaaat aaaaagtcat aaatttaaaa ttatagggat cttttctggt
      841 aaaaaacagg aaacatatac aggattatct tctgatttta gtgaaaatat ggtgtttgta
      901 gattattcaa caagccaaga gatattaaat aattcagaaa ataatgaaat tgcaaataaa
      961 attttaatgt attctggtag tgcagaatct acagacttag ccttaaacaa attgaaagag
     1021 cttaaaattg atgagtcaaa gtattttgtt gaaaaagatt ctaatgcgtt tgaagagtct
     1081 ttagagtcag tgagtggaat aaaatatata ataaaagtaa tgacttattc tattatgtta
     1141 ggtgggat
//
LOCUS       DACEXQ010000368         1148 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.384, whole genome shotgun sequence.
ACCESSION   DACEXQ010000368 DACEXQ010000000
VERSION     DACEXQ010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.384"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..>1148
                     /locus_tag="IXH04_002841"
     rRNA            <1..>1148
                     /locus_tag="IXH04_002841"
                     /product="23S ribosomal RNA"
BASE COUNT          350 a          227 c          329 g          242 t
ORIGIN      
        1 ttggggagct gtacgtaagc tatgatccag agatttccga atgggggaac ccaacatctt
       61 taataggatg ttacgattgt gtgaatacat agcacattcg aggtagacgc agagaactga
      121 aacatctaag tacctgcagg aagagaaaga aaattcgatt ccctgagtag cggcgagcga
      181 aacgggaaaa gcccaaacca gcaagcttgc ttgttggggt tgtaggactc caatatggta
      241 gttctttcag atagtcgaat gacttggaaa agtcagtcaa agagggtaaa aaccccgtag
      301 acgaaatgtg gaagacacct aggaggatcc tgagtacggc ggaacacgag aaattccgtc
      361 ggaatccggg aggaccatct cccaaggcta aatactccct agtgaccgat agtgaaccag
      421 taccgtgagg gaaaggtgaa aagcaccccg gaaggggagt gaaatagaac ctgaaaccgt
      481 gtgcctacaa caagtcaaag cccgttaatg ggtgatggcg tgccttttgt agaatgaacc
      541 ggcgagttac gattgcatgc gaggttaagt tgaagagacg gagccgcagc gaaagcgagt
      601 ctgaataggg cgtttgagta tgtagtcgta gacccgaaac catgtgatct acccatgtcc
      661 aggttgaagg tgcggtaaaa cgcactggag gaccgaaccc acgtacgttg aaaagtgcgg
      721 ggatgaggtg tgggtagcgg agaaattcca aacgaacttg gagatagctg gttctctccg
      781 aaatagcttt agggctagcc tcggaattga gaatgatgga ggtagagcac tgtttggact
      841 aggggcccat ctcgggttac cgaattcaga taaactccga atgccattca ttcatatccg
      901 ggagtcagac tgtgagtgat aagatccata gtcgaaaggg aaacagccca gaccaccagc
      961 taaggtccca aaatatatgt taagtggaaa aggatgtggg gttgcacaga caactaggat
     1021 gttggcttag aagcagccac catttaaaga gtgcgtaata gctcactagt cgagtgaccc
     1081 tgcgccgaaa atgtaccggg gctaaacata ttaccgaagc tgtggagtac acctttaggt
     1141 gtattggt
//
LOCUS       DACEXQ010000369         1125 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.386, whole genome shotgun sequence.
ACCESSION   DACEXQ010000369 DACEXQ010000000
VERSION     DACEXQ010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.386"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..343
                     /locus_tag="IXH04_002842"
     CDS             <1..343
                     /locus_tag="IXH04_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853374.1"
                     /translation="NIKTSTKLICKLFKLDKISNIDDVKSKILTRKVSEPVKLTAQDK
                     KNGIVLYGDDAKYNYNWDTSSEAEVLSIPTTDTSGEKVTFKINFITAISKLEYGKQKK
                     RDNNDQYLPKK"
     gene            424..1044
                     /locus_tag="IXH04_002843"
     CDS             424..1044
                     /locus_tag="IXH04_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853375.1"
                     /translation="MNINRVIKLIGAKNINKVRSEFIFPEDFFNWFFYQFSECKRLLG
                     ENYYLKGIGGFMGNVADEQNIIKGISEQTSELTVTKAFISNGESLKNVTARIRKENEV
                     DIVFGIDHECNSVIYVTQSANLILFENGCKDDFMVVYLYDILIPKLRSLYMSSESIFV
                     SEQKGSFSKKIGIEVIESIMCHNHITLEELEILIGQKNNNTKIQVS"
BASE COUNT          442 a          131 c          196 g          356 t
ORIGIN      
        1 aaatataaaa actagtacga aattaatttg taaattattt aagcttgata aaataagcaa
       61 tatcgatgat gtaaagtcca agatattaac aagaaaagta agcgaaccag taaagctaac
      121 ggcacaagat aaaaaaaatg gaattgttct ttatggtgat gatgcaaagt ataactataa
      181 ttgggatacc tcctcagaag ctgaggtatt aagtattcct actaccgata cttcagggga
      241 aaaagtcacc tttaaaataa attttataac ggcaatttct aagttggagt atgggaagca
      301 aaaaaagaga gataacaatg atcaatatct acctaaaaaa taacgaatta atgatgcata
      361 tgttaaagtt gtttttttga attaaacaac tcaatttatt cagttatatg ttcatcgcag
      421 caagtgaaca ttaatcgagt tataaaatta ataggagcta aaaatataaa taaagttcga
      481 tcagaattta tttttccaga agattttttt aactggtttt tttatcaatt cagtgaatgc
      541 aagcgactct taggtgagaa ttattactta aaaggaatcg gcggatttat ggggaatgtg
      601 gctgatgaac aaaatattat aaaaggaata tctgaacaaa catcagagct tacagtaact
      661 aaagcattta taagtaatgg tgaaagtttg aaaaatgtaa ctgcgcgaat tagaaaagaa
      721 aatgaagtag acattgtttt tggaattgac catgaatgta attcagtgat atatgtaaca
      781 caatcagcaa atttaattct ttttgagaat ggttgcaagg acgattttat ggtggtctat
      841 ttgtacgata tactaattcc aaaacttcgt tccttatata tgtcttctga aagtattttt
      901 gtgagtgaac aaaagggatc tttctcgaaa aaaataggta ttgaagtaat tgaatcgatt
      961 atgtgtcata atcatattac gctagaagaa ttagaaattt tgatagggca aaaaaataat
     1021 aacacgaaaa tacaagtttc ttagaaatca ttttttattg agatatctag ataaatgatt
     1081 tattcttcaa tcaaccaact cgccaagctg aggtaatgtt ttgag
//
LOCUS       DACEXQ010000370         1109 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.388, whole genome shotgun sequence.
ACCESSION   DACEXQ010000370 DACEXQ010000000
VERSION     DACEXQ010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.388"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            5..>1109
                     /locus_tag="IXH04_002844"
     CDS             5..>1109
                     /locus_tag="IXH04_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853376.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQAL"
BASE COUNT          473 a          176 c          247 g          213 t
ORIGIN      
        1 ggacatggca gaagaaaaag aaccactaga caatagtcgc ttgggaaaat ccaaaagaaa
       61 attagtacga ttgcaaaatg aattaaacga acaaattgag aagatgtttg aacaccagag
      121 aaagaccaac ggtcagccaa tgaacgataa gagaaatggg cattcttggt ttaggcaaca
      181 agaacgttta gaaaacaaag tgcatagtct gagagaagag atcaagcaac aagaaaaaca
      241 ggtagaaaaa cttgaaagac aagaagagct aaaggaaatg ggctataaca agtacggcgg
      301 cctggatatg acaatcgaaa atattccaag aatcaaagaa gaaatcgaac gctttgaaaa
      361 aggtgaatcg actttttcag ccgctacgat ccgaaaatac caacggaaat tggaaacatt
      421 agaacagctg aaagaaagat cagaaaaagg aaaagaaaat cttcttcctg aagtacaagc
      481 aattattgac agcggacgag tgacgcaatg gaaaaaaaat ccaaccattt attttttgaa
      541 agactatcga aaagttgctt tagaactgaa tgaaaacggg ggatttgagg aatcaaaaaa
      601 ataccaagcc acgtcggaag aggaaaaagc agctgttcgt gaattacttg aagaggctgc
      661 gccaactgat tctttgaagg agaaagaaaa cgatcatttc tatcgagtag aattcaacga
      721 aaatgatcca gaaaaaggat tgaaaagtta tggtggggag atcgtaacat tagaattgat
      781 cgatcaactc aaaacgttgg ataaccaata cgccgcagaa gcaggttatt ataaattcta
      841 ttttgaagaa gtgaagaacg gagaagtagt cgatcagatc cgaatggatc taggggatgg
      901 cgtcaacagt aatcaaccca tctatttaga gttagcaaaa ttgtgcgaac aagtacctga
      961 aaaggaacaa aaacaaacgg tcaaaaatga acctgtttcg aaagaacaaa tgcagtcgaa
     1021 agaaacaaaa acaaaacaaa agggctatca aaaacgaacg gcagaagaga ttaagcaaga
     1081 agtcaaatcc ctttcgcaac aggcactgg
//
LOCUS       DACEXQ010000371         1102 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.389, whole genome shotgun sequence.
ACCESSION   DACEXQ010000371 DACEXQ010000000
VERSION     DACEXQ010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.389"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..484
                     /locus_tag="IXH04_002845"
     CDS             <1..484
                     /locus_tag="IXH04_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1853377.1"
                     /translation="TKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLV
                     APQFESPNRLLLTFQNIYFYCVPSKIFEKLGLSKKQTLPLLRSLETLDCKKQLRTSRL
                     PKLILCGTKDHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS
                     "
     gene            complement(598..972)
                     /locus_tag="IXH04_002846"
     CDS             complement(598..972)
                     /locus_tag="IXH04_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ1853378.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
BASE COUNT          376 a          198 c          153 g          375 t
ORIGIN      
        1 aaccaaatac atcgaagtac aattgaatca gataaaggaa ccttttattt tatgtggatt
       61 atctttaggc gctgttattg ctttaaacta tcttgcaact ccttctccat atttaaaaaa
      121 ggttgtttta gtcgctccac aatttgagtc tcctaatcgc ttattgctta ctttccagaa
      181 tatttatttt tactgcgtac cttctaagat atttgaaaaa ttaggtctat ctaaaaaaca
      241 aacccttccc ctacttcgtt cacttgaaac attagactgc aaaaaacagc ttcgtacatc
      301 acgacttcct aaattgattt tatgcggtac caaagatcat gcgaataaac cagcagcaaa
      361 acgtttatat aaactttcaa cgaatggccg gctactcttt attaaaaacg gaaaacatga
      421 attgaataga gaaaagccag aaataatggc acaagctatg cgatctttta tcgaaaatag
      481 ttaatttaaa aaaagacgac aaaatgtgtc gtctttttat ttaccatggt aagaatgttg
      541 tcttgttttt tcaatacagc ttgattgaga ctagttttat ggacaataga gagaaagtta
      601 ttgttttatt ccagagaatt tttctggtcg tagatctact aattgaacat gactgtctaa
      661 agaaccactt gggctttcaa gagtaaatac acctagacgt ggatctaaag aaagggtcat
      721 atgttcatcc atgtacattt ttgaataatc ttttatataa acatccccaa tatcactatc
      781 taattggagt ttataatcat tttttctctg ttctttatca agaagaagta aacgaaaact
      841 agtatctaaa gagcaaacac ccattttaga atattcccca acgccatcat ctaaatccaa
      901 tataactgtt gcgccttcat caatatattt ttgtaatctt tcttgtgctt gttccgtaat
      961 tttaagatac ataactaata cgccctcttt ctttttctta caaaagcatg ctagcatata
     1021 ttaataaaat tatctaaagt cctgctcaaa acaaaaagat gtaagaaata tcttatgata
     1081 tttcttacat ctcagagtgt ag
//
LOCUS       DACEXQ010000372         1054 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.391, whole genome shotgun sequence.
ACCESSION   DACEXQ010000372 DACEXQ010000000
VERSION     DACEXQ010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.391"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..894
                     /locus_tag="IXH04_002847"
     CDS             <1..894
                     /locus_tag="IXH04_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAQ1853379.1"
                     /translation="KAIPSFWGGSADLSGSNNTMVAADKDFTPEHYEGRNIWFGVREF
                     AMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAIQHTPVVYVLTHDSVAVGEDG
                     PTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAMETTDAPTILVLSRQNLPVLPS
                     TKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLAVKAQKELAEKGKDVSVVSMP
                     SFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERYVGTEGATITIDHFGASAPGT
                     KILEEFGFTVENVVNTYNQLS"
BASE COUNT          314 a          201 c          237 g          302 t
ORIGIN      
        1 aaagctatac caagcttctg gggtggttca gcggacttgt caggttccaa taatacaatg
       61 gtggctgcag ataaagattt cactcctgaa cattatgaag gacgcaacat ctggttcggt
      121 gtccgcgaat ttgcaatggc ttcggctatg aatggtatcc aacttcatgg cggaactcgt
      181 atctacggtg gaacattctt tgtctttgtc gattatctaa gaccagcagt tcgtttagca
      241 gcaatccaac atactcctgt tgtttacgtc ttaacacatg actcggttgc tgtcggagaa
      301 gacggaccaa cgcacgaacc gattgaacaa ctagcatctg ttcgctgcat gcctggtgtt
      361 caagtgattc gtccggcaga cggaaatgaa acacgtgctg cttggaaagt agcgatggag
      421 acaacagatg cgcctactat ccttgtattg agtcgtcaaa accttccagt attgccttct
      481 acaaaagaag tagcagatga aatggtgaaa aaaggcgcgt atgttctttc tccatcacaa
      541 ggagaaacac cagaaggtat tttgattgca acaggttctg aagttgattt ggctgtgaaa
      601 gctcaaaaag aattggctga aaaaggcaaa gatgtttctg ttgtttctat gccaagcttt
      661 gacctctttg aaaaacaatc ttctgaatat aaagaaagtg tacttcctaa atcagtgaag
      721 aaacgtgtgg ctatcgaagc tgctgcatca tttggatggg aacgctacgt aggaacagag
      781 ggcgcaacga ttacaatcga tcatttcggt gcatctgccc ctggcactaa aattttagaa
      841 gaatttggat ttacagtaga aaatgtcgtg aatacgtaca atcaactatc atagatccaa
      901 gcaattgttt gactgataag aacagctgtc tgcaagttgg agacagctgt tctttatgtg
      961 ttagaagaat aaatcaatat tacttttcgt tttcggctat gttacattta aatgacaaaa
     1021 caaagaattc ttcggattag tttgtgtttc ttga
//
LOCUS       DACEXQ010000373         1037 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.392, whole genome shotgun sequence.
ACCESSION   DACEXQ010000373 DACEXQ010000000
VERSION     DACEXQ010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.392"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(20..91)
                     /locus_tag="IXH04_002848"
     tRNA            complement(20..91)
                     /locus_tag="IXH04_002848"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="IXH04_002849"
     tRNA            complement(113..200)
                     /locus_tag="IXH04_002849"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="IXH04_002850"
     tRNA            complement(225..298)
                     /locus_tag="IXH04_002850"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="IXH04_002851"
     tRNA            complement(312..382)
                     /locus_tag="IXH04_002851"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="IXH04_002852"
     tRNA            complement(384..459)
                     /locus_tag="IXH04_002852"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
     gene            complement(478..551)
                     /locus_tag="IXH04_002853"
     tRNA            complement(478..551)
                     /locus_tag="IXH04_002853"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(515..517),aa:Met,seq:cat)
     gene            complement(564..653)
                     /locus_tag="IXH04_002854"
     tRNA            complement(564..653)
                     /locus_tag="IXH04_002854"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(615..617),aa:Ser,seq:tga)
     gene            complement(677..750)
                     /locus_tag="IXH04_002855"
     tRNA            complement(677..750)
                     /locus_tag="IXH04_002855"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(714..716),aa:Ile,seq:cat)
     gene            complement(764..837)
                     /locus_tag="IXH04_002856"
     tRNA            complement(764..837)
                     /locus_tag="IXH04_002856"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(801..803),aa:Met,seq:cat)
     gene            complement(865..937)
                     /locus_tag="IXH04_002857"
     tRNA            complement(865..937)
                     /locus_tag="IXH04_002857"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(902..904),aa:Ala,seq:tgc)
     gene            complement(957..1030)
                     /locus_tag="IXH04_002858"
     tRNA            complement(957..1030)
                     /locus_tag="IXH04_002858"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(994..996),aa:Pro,seq:tgg)
BASE COUNT          281 a          285 c          246 g          225 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca gtgtttttag tttttaattg
      481 cgggagcagg atttgaacct acgaccttcg ggttatgagc ccgacgagct accagactgc
      541 tccatcccgc gataatatga aaaaggagga taagggattc gaacccttgc acggttttac
      601 ccgcctgacg gttttcaaga ccgttccctt cagccggact tgggtaatcc tccatatgaa
      661 acttacaacg ccacaatgga ccttgtagga ctcgaaccta cgaccggacg gttatgagcc
      721 gtctgctcta accaactgag ctaaaggtcc aggctcttaa aaatcgcggc ggaggggatc
      781 gaacccccga cctcccgggt atgaaccgga cgctctagcc agctgagcta caccgcgaaa
      841 aagatattca ttatttaaat gaaatggagc ctagcgggat cgaaccgctg acctcctgcg
      901 tgcaaagcag gcgctctccc agctgagcta aggccccgtg tttaaattta taattatcgg
      961 gaagacagga ttcgaacctg cgaccccttg gtcccaaacc aagtgctcta ccaagctgag
     1021 ctacttcccg tttaatt
//
LOCUS       DACEXQ010000374         1035 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.393, whole genome shotgun sequence.
ACCESSION   DACEXQ010000374 DACEXQ010000000
VERSION     DACEXQ010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.393"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            116..1009
                     /locus_tag="IXH04_002859"
     CDS             116..1009
                     /locus_tag="IXH04_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAQ1853380.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
BASE COUNT          381 a          166 c          187 g          301 t
ORIGIN      
        1 atactaaagg ccgaagaaat ggaggagaat aacagtcaat tatgactctt gttcttcctc
       61 ctttttacgt aaaagccttt taagtaaaat aaaacggata gaaaagagaa catatatgaa
      121 ctattattca aaaaataagg aaaactttta tcaaaaactg accgatgatc cgcttttttc
      181 ttcattgacc gattatctat atgaacatcg agaacaagaa acgattctaa gagaattgaa
      241 aaaagaattt tcgcaaaata aattttccca ttttcttgat ttattgatcg atgcaggatt
      301 aatcaagagg gaagaacgaa gatatcattt gaactttcca atatttgatc ctaaagatta
      361 cttgcaacaa gccacttcgg ctgctgaaac catagctgaa caattgaaga gactctctgt
      421 tgatgaacaa aaattagcga tgggagaagt tatttgggcg tattgtttcg aagatgaacg
      481 gaaagaagcg tatttttatg gagtaagaaa cagccgagag acagaattgc tgaggacaac
      541 tgcaggaaat gagaagtacc gttttatcac actttcctct atagaacact tcccgcttac
      601 tttggctaat tacttttttg tacagaagaa tcagcttcct gtaacgaagg cattcaaaga
      661 attagccgaa cttattggag acgtaaatga atcgtacttt tttgatcaaa tagaagttat
      721 cgttgacagg atacgtaaga ataaatataa aaatcgccgg cctagtattt tccatcagtc
      781 tctacttgtg acgaatacta taaaggaaga agaatcattc acattagaat tacctattgt
      841 cgaaaaaaac aattttgaga ttgaattacc aacattggat ccttcattga caatggagga
      901 aatggccttt cttaaacgac aaatcttttc tgagctgagc aagaaattta taccgcatgc
      961 attttcttat atcaaagaat atggaacagt tttagtgagt aaaacctaag agagcagtaa
     1021 gcaaaaatta tactg
//
LOCUS       DACEXQ010000375         1033 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.394, whole genome shotgun sequence.
ACCESSION   DACEXQ010000375 DACEXQ010000000
VERSION     DACEXQ010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.394"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..342)
                     /gene="rbgA"
                     /locus_tag="IXH04_002860"
                     /gene_synonym="ylqF"
     CDS             complement(<1..342)
                     /gene="rbgA"
                     /locus_tag="IXH04_002860"
                     /gene_synonym="ylqF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="essential GTPase; functions in ribosome assembly;
                     binds a unique part of the 23S rRNA; interacts with
                     ribosomal protein L25(Ctc); Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAQ1853381.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAAR"
     gene            602..1000
                     /locus_tag="IXH04_002861"
     CDS             602..1000
                     /locus_tag="IXH04_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ1853382.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
BASE COUNT          318 a          168 c          186 g          361 t
ORIGIN      
        1 ccgagcagca tctcttgccc gtttttcttt caatgcttct ttagccgcag gtactagacc
       61 tttaattcct ttactttctt gtgccgtgat agctaatgca agatggcctt gtttacgaaa
      121 atattcttgc cacagctttg tctgttgtgg atctgccaga tccgctttat tcaataaaac
      181 aaggcgaggt ttttgctgaa tgatctgatc tagcattgga ttacgagaag ataaaggtaa
      241 tcgtgcgtct accaattcaa atacgatgtc cacatatttc attttttcag aaacttctcg
      301 tcgggctttt gccatatgtc ccggaaacca ttggatggtc attgttctct ccattccttt
      361 cataccggaa tattccccgg ctttacttgt ttttttcttt ttcaatataa attcactttt
      421 ttattataaa gaaattccat ctattagtta agtgaaagtg aaccagtcct cattctatca
      481 catcttcact tttttgatga tgcaaatttt tattttggtt tctatatatg aatctaaatt
      541 tttgacagcg tttcctaaat aagagatgat agacgaacaa aacagaaagg agacgaaaca
      601 aatgaatagt atggtttttg ttaattttcc tgttgctgat gtcaatcgtt ccgttgcttt
      661 ttatgaaaag ctgggtttta aaaaaaatga agaattttct actgaagaag ctggttcaat
      721 ggtatgggat gacacttttt ggataatgct tctctctcat gatttttata aaaaatttat
      781 aaaagataaa gaaatagctg ataataaaaa aacaagtggt gctttgattt cgtttagtgt
      841 tgaaaatgcg gatgaagtaa aaaaatttgc tcaaattgca aaagaaaatg gtggtacttt
      901 ccacgaagta gatatgggaa tgcctgaaga agaaatgttt tcattagaag tccaagatct
      961 agacggaaat acattagaac ccgtatggat gaagatgtaa gcagataact aatctgacgt
     1021 tttttataac aaa
//
LOCUS       DACEXQ010000376         1026 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.395, whole genome shotgun sequence.
ACCESSION   DACEXQ010000376 DACEXQ010000000
VERSION     DACEXQ010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.395"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..>1026)
                     /locus_tag="IXH04_002862"
     CDS             complement(<1..>1026)
                     /locus_tag="IXH04_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1853383.1"
                     /translation="SVLGIIIILVHSLIFFIFPKTYLETRKEKIHNMANEISSNMNGK
                     EIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLESDSNSLIIEEREIKLN
                     DGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISLIYAKSIKNNIQEIKI
                     VTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDLEFKNKEILKLEKLKY
                     DFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIEIVDSLTKNISQILSV
                     HSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILDENVYIGKTALKIILS
                     NLISNAV"
BASE COUNT          343 a          132 c           86 g          465 t
ORIGIN      
        1 ttaccgcatt actaatcaaa ttagatagaa taatctttaa agctgttttt cctatataaa
       61 cattctcgtc taatatataa ttattgacag ttattttctt ttgatttgct aatatttcat
      121 acttttttaa tatgtcttct aatgtatcat ttattttcaa atattcttca tcattattca
      181 aattttctat agaatgaact gataatattt gagaaatatt ttttgttaaa ctatcaacga
      241 tttcaataca ttcattaata taaatatctc tttctttata ttttccaata ttgtatttca
      301 tgttttccag tattatttta agactagcaa gaggggtttt aagttcatgg gatgcacctt
      361 taaaaaagtc atattttaac ttttctaatt ttaaaatttc tttattttta aattccaaat
      421 cgtcaattgt tcttaataaa gtagaataca aatcattaat ttgttgtttt aattctccaa
      481 cttcatcatt agagctaact tttaaactca tttttttatc aagtttcatc attttatcag
      541 taacaatttt tatttcttgt atattatttt ttattgattt tgcataaatt aaagaaataa
      601 tagcagaaaa taaaattgat attaataaag aatatggtaa aaatttaagg cttaaatctt
      661 tcgcatcttt ttgcatatca gctgtagaaa caaattgtag atttgttttt ttaccatcat
      721 taagctttat ttctctttct tcaattatca aagaattact atcactttct aaattaacat
      781 ttatattatc tttgatttgt atttcattct tattattttt ttcttttatg aatactttta
      841 tttcactact cttggaataa aactccaaag tttgttctat atattttatt tcttttccat
      901 tcatattact agaaatttca tttgccatat tatgaatctt ttcttttctc gtctccaaat
      961 atgttttagg aaagataaaa aaaattaatg aatgaactaa tataattatt attccaagaa
     1021 cagaaa
//
LOCUS       DACEXQ010000377         1024 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.396, whole genome shotgun sequence.
ACCESSION   DACEXQ010000377 DACEXQ010000000
VERSION     DACEXQ010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.396"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..307
                     /locus_tag="IXH04_002863"
     CDS             <1..307
                     /locus_tag="IXH04_002863"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="catalyzes the addition and repair of the
                     3'-terminal CCA sequence in tRNA; these proteins belong to
                     the CCA-adding enzyme subfamily 2 which does not have
                     phosphohydrolase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAQ1853384.1"
                     /translation="ALEPFELYFLGKKHALQVEELMIYFDETPQMDAVGESYDRLPIK
                     SLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQVLDFLSKEKLI"
     gene            461..916
                     /locus_tag="IXH04_002864"
     CDS             461..916
                     /locus_tag="IXH04_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853385.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
BASE COUNT          373 a          164 c          212 g          275 t
ORIGIN      
        1 agctttggaa ccattcgaat tgtacttctt aggcaaaaaa catgcactgc aagtagaaga
       61 attgatgatc tattttgacg aaacacctca gatggatgcc gtaggagaat cctatgatcg
      121 tttacctatc aagtcattaa gcgaattagc agtagatggt aaaatcttgc tagaagaaac
      181 tggaaaaaaa cctggtaagt gggttagtga agctttgcaa ttagctgaaa aagccgtcgt
      241 agaacaaaga ataaaaaatg ataaaaaaca ggttttggat tttttatcga aagaaaaatt
      301 gatttaaaaa acactagact gtatctgctc agctttgttg gctacttaaa gtgggcagat
      361 acgtcattag aagaaaaaac ataagatgtg accacttaca taaatttgtg ctaaactgag
      421 gtagtacaag aataaattac atcataaagg agtcttttta atgagtaaag aaatgacagc
      481 gctaaaattt tattttagaa atggagaaac ctggacgatc aaccgccgtc atatcggcga
      541 tctttggatc aaacaaatca caacaagttt tggacgaatc aatggcagtg aatttgttga
      601 aatccatccg tgtgctggat ttaaaatcga aatcttccat gaaggggacg cagttgcgac
      661 acatgatatc aacctaggcg gactggaaat gggcatgttt aatcgcgcat tgaaatacga
      721 agatatcgag cgaatggaaa tcctttaccg aaatggcaca cctgatcttg tgtacttccc
      781 atatctagat aaaggaacag aaggattaga caatcaatat caatcaacaa aaatcagcga
      841 aaaaacaggt aacttgtata tcgtgatcaa tccagatcaa cgtgtagaag atgtatacgg
      901 ggaatttttc gaataaatcg atttttacgc aaaaatgagg ctgtgaaaga gatgtgtctt
      961 attactactg ataggctgtc tcttccacac cgtttattca gaataaggga ctgtgacaag
     1021 attt
//
LOCUS       DACEXQ010000378         1012 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.397, whole genome shotgun sequence.
ACCESSION   DACEXQ010000378 DACEXQ010000000
VERSION     DACEXQ010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.397"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..195
                     /locus_tag="IXH04_002865"
     CDS             <1..195
                     /locus_tag="IXH04_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor protein"
                     /protein_id="HAQ1853386.1"
                     /translation="ITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIY
                     ADYDNFYIYFYLESVGEYGD"
     gene            185..586
                     /locus_tag="IXH04_002866"
     CDS             185..586
                     /locus_tag="IXH04_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853387.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            579..983
                     /locus_tag="IXH04_002867"
     CDS             579..983
                     /locus_tag="IXH04_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853388.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
BASE COUNT          371 a          155 c          191 g          295 t
ORIGIN      
        1 ataacagcta tggatgcgat gggatcgaaa ttaactaaga aagtaaagac tccatttcat
       61 ccaatcgcca agaaatttaa taaagatcaa tattttatcg taatcgatag tatgcgttac
      121 aacgttatct atgccgatta cgataatttt tatatctatt tttatcttga aagtgtgggt
      181 gaatatggtg attgataatt ctaaagaaaa agaacgttta aataagcaaa tttctgctat
      241 caaaacttcc ttagaagagc attttggcct caaactcttt caagactccg taggcgagga
      301 tgagctacct gatgatttta attacttcat tctcgaaaca ggagaaatag aaatgatcac
      361 tgagccaaaa tatagcgtgg gtcaaaatct atatctaact ttctattcag aaaatagaga
      421 agatttaaca ggagactcac tagatattat ttcattgatt caaaatcgtt cgattcgttt
      481 tcagagaatg gatcccaatc atttaaaact agagaaccaa gatcgctata tcgatcaatt
      541 ggtatttacg tttagacgat tattgaagag tgattgtcat ggctaaaaat agttgggagc
      601 taaaaataaa tggacatgat gaacttcttg tgcggatgga acgctattca agcgagagcg
      661 aacgactgat taacgaagca ttgaaatcaa aaggttcggc tattgcagtg gataggatta
      721 cagaaaaaat tcctgtttct gaagcagatt taagaagagg gcaccaacac gcaaaaaata
      781 gtcgtccact taagactcaa tacattaatt tgggtttcat cattagacct acaagaaaat
      841 ttgagtattt aaaatatcct gatttgggga taggtacttc taaaagaaat cagccggacg
      901 aatttatgag aagaggatta ggtcttgcac ttgatccaat tacagaactt ctgattcgtc
      961 aattcgataa attaaataaa tagggggaac aacaatggct aaaacaaaaa ct
//
LOCUS       DACEXQ010000379          985 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.398, whole genome shotgun sequence.
ACCESSION   DACEXQ010000379 DACEXQ010000000
VERSION     DACEXQ010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.398"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..572
                     /locus_tag="IXH04_002868"
     CDS             <1..572
                     /locus_tag="IXH04_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853389.1"
                     /translation="RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANI
                     VNVTVESLETINTNSGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEM
                     WAQLWNMMYFNIGPKVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVT
                     STPFDEDLSFVNKKKCSYAYVKAIKAVVR"
     gene            569..880
                     /locus_tag="IXH04_002869"
     CDS             569..880
                     /locus_tag="IXH04_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853390.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            870..>985
                     /locus_tag="IXH04_002870"
     CDS             870..>985
                     /locus_tag="IXH04_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor protein"
                     /protein_id="HAQ1853391.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTE"
BASE COUNT          359 a          135 c          206 g          285 t
ORIGIN      
        1 aaagaattaa tttgcgcaaa ttgccttcta aagatgatgt ttggtattgt agcgactgct
       61 gtagttatct aagtcttgat tatattagaa gctgtgaaaa cggagaaaat attctaaaag
      121 atatggcaaa cattgtaaat gttacagtag aatctttgga aactataaac actaattcag
      181 gaggcgcaca gtgggttatt aaccgtccta aagcgaatta ttggaaaaag gtttatctgg
      241 attctaatcg gctatatcgc taccttagag ggcaaaaaac aaatatacaa atctggacag
      301 ccgagatgtg ggcacagctt tggaacatga tgtatttcaa tattggtcct aaagttcacg
      361 aggaattaga cttttgtttt gctactgatc caatagaaaa agttaaagaa gtaaaaatct
      421 tacacaatgc tggagtaaca acaaacgatg aagatttatt tttcaaaggg agatacgtga
      481 cttctacgcc ttttgatgaa gatttatcat ttgtaaacaa gaaaaaatgc tcttacgcat
      541 atgttaaagc aattaaggcg gtggttagat gacgcctgaa caagtgactg aagaattgct
      601 aatagctgtg aaggataata tttacgttac ctggaacgaa gaagatgagt caattaaaaa
      661 gatgatagct aaaaatgctg tttatcttca aagtaaagtg agtacaacac tttctttttc
      721 tcctgaaagc ttagaatacg gattgctaat cgaaagatgt agatacgact ggaatcgtgc
      781 tttagatgag tttgaacaaa atttcgctag tgagttatta ggtttcattc aacattatgc
      841 gctacaagaa tatattgcag gtgatgtgaa tggcgaataa tcgtagactc gaagaaacat
      901 tcaacgatgg ttggttaaag attttgacgc aaactaccaa aagaaatgaa ctaggaaaaa
      961 agattggtgt agaagataca gaaat
//
LOCUS       DACEXQ010000380          978 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.399, whole genome shotgun sequence.
ACCESSION   DACEXQ010000380 DACEXQ010000000
VERSION     DACEXQ010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.399"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..>978
                     /locus_tag="IXH04_002871"
     CDS             <1..>978
                     /locus_tag="IXH04_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
                     /protein_id="HAQ1853392.1"
                     /translation="RNMQVNNIFRRYVADEDLLIYSIDESILKVTKSLNLFTTEETRS
                     QRRKKLAQMIQERIKEELGLIATVGVGDNPLLAKLALDNEAKHNEGFIAEWTYENVPE
                     KVWNIPEMTDFWGIGSRMKKRLNQMGILSIRDLANWNPYTIKNRLGVIGLQLYFHANG
                     IDRTDIAIPPEPTKEKSYGNSQVLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGC
                     VHLHIGTSILETRPGFSHQMKIPITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKL
                     VYTDSLQLDLFSDPQKQIDEENLDKIIDKIRQKYGFTSIVHASSMLEGA"
BASE COUNT          346 a          156 c          218 g          258 t
ORIGIN      
        1 gcgaaatatg caggtaaata atattttcag aagatatgtg gctgatgaag atctactgat
       61 ttactcgatc gatgaatcga ttttaaaagt gacaaaatca ctgaatcttt ttacgactga
      121 agaaacacga agtcagcgaa ggaagaagct cgctcaaatg atccaagaac gtattaaaga
      181 agagttaggt ttgattgcta cggtaggcgt cggcgataat ccccttttgg ctaaattagc
      241 cttagacaat gaagcaaagc ataatgaagg atttattgcg gaatggacgt acgaaaacgt
      301 tcctgagaaa gtatggaata ttcccgaaat gacggacttt tggggaattg gttcgcgaat
      361 gaaaaaaagg ctcaaccaaa tgggaatttt gagcattaga gaccttgcta actggaatcc
      421 ttatacgatt aagaatagat taggtgtgat tggattacag ctgtacttcc atgctaatgg
      481 gatcgatcga acagatatag caatcccacc agaaccgaca aaggaaaaat catatggaaa
      541 ttcacaagtg ttaccacgag attatactag aagaaatgaa atcgagctgg tggtaaaaga
      601 aatggcggaa caagtagcga tacgcattag gcagcataac tgtaaaacag gttgtgtcca
      661 tttacacatt ggcacgtcga tcttagaaac gagaccaggg ttttcacacc agatgaagat
      721 ccctatcaca gataatacaa aggaattgca gaattattgt cttttcttgt ttgataagta
      781 ttatgaagga caagaagtaa ggcatgttgg aataacctat agcaagcttg tgtacaccga
      841 ttcgcttcag ctggatttat ttagtgatcc acaaaagcaa attgacgaag aaaatttaga
      901 taaaattatt gacaagattc ggcaaaaata tggttttacg tcgattgtac atgcttcttc
      961 aatgttagaa ggcgctag
//
LOCUS       DACEXQ010000381          973 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.400, whole genome shotgun sequence.
ACCESSION   DACEXQ010000381 DACEXQ010000000
VERSION     DACEXQ010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..973
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.400"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..630
                     /locus_tag="IXH04_002872"
     CDS             <1..630
                     /locus_tag="IXH04_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAQ1853393.1"
                     /translation="PNKISQFLAGSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQ
                     YRVVDPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQETKRKIVPPEIKTWWR
                     LELQLRRGKATDWYAMVHESLDSFASPHFLPTDTKHTDRIMIAGLISDQNLWAGLARH
                     TKYKYRNLLKKESQSDELVNHLRETFSESADVLKKELDTWLLGLDVTEE"
BASE COUNT          307 a          155 c          234 g          277 t
ORIGIN      
        1 ccaaataaaa ttagccaatt tttggcgggt tcgatgaaga actttatcca tgatttattt
       61 ttagaaccac atttctcacg tgctgatatt gcttgcgaca ttatcgatgt gcctgatgat
      121 tttattacgc aatatcgtgt ggttgaccct gtttcattca aaccaatcta tgggcggtct
      181 gggaagctgg aaacggcata ttggggtagt cgtgccagtg agcgacaaat aaggatgtac
      241 aacaaaaaat tggaacaaga aaccaaacgg aaaattgtac caccggaaat aaaaacttgg
      301 tggcgactgg aattgcagtt acggcgtgga aaagctactg attggtatgc gatggttcat
      361 gaaagtttag acagtttcgc aagtcctcat tttttgccga ctgatacaaa gcatactgat
      421 agaattatga ttgcaggatt gatttcagat caaaatttat gggctggctt ggctcgtcat
      481 acaaaatata aatatcgaaa tttgttgaaa aaagaaagcc aaagtgatga gttagttaat
      541 catttacgag agacattttc ggaatcggca gatgttttga aaaaagaatt ggatacgtgg
      601 cttttggggt tggacgtgac ggaagaatga gtggatgaat ttttgtccac ttttttattt
      661 tcagcacaag tcggcatggt gggtcgtgcg tttgcagcaa gagcgaaaaa acgctaacga
      721 atccgcaaat gccgacttgg ataaaacagt tgcgcaaaga gacttggctt tttataataa
      781 atttagggat atcccagtgt cactctttgg ggtaattcgg gggaaggagt tacccctcac
      841 tctggaaaat tagagcgaag cagacgagat tatcccaatg aatatgacgg cttactcgga
      901 gtgataccga gtaaaaaagt cagataagtg agagtaaatt tacgtcaatg tttaagataa
      961 gcgattattt tgc
//
LOCUS       DACEXQ010000382          971 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.401, whole genome shotgun sequence.
ACCESSION   DACEXQ010000382 DACEXQ010000000
VERSION     DACEXQ010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..971
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.401"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            207..407
                     /locus_tag="IXH04_002873"
     CDS             207..407
                     /locus_tag="IXH04_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1853394.1"
                     /translation="MITLNDQFIRSLRRHRADLILTKNDAAKLIGINRKTYVKIENGS
                     KESIRASTYQKLVNWLLNDLKI"
     gene            419..745
                     /locus_tag="IXH04_002874"
     CDS             419..745
                     /locus_tag="IXH04_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853395.1"
                     /translation="MASWLIEEIENERRKIMDAGITVMLDKQQTNQLKNYVFEMTKEA
                     IDQARIDTGLERPFLKGKEMAKYLNVSYTTFLKFKRMGLPVILLEKMELFSKEECKKW
                     ILSHQI"
BASE COUNT          405 a          107 c          171 g          288 t
ORIGIN      
        1 actaatttgt ttaatggtct ttttactgga atcaatataa aattttattc tattcaaagt
       61 aacgccccct atagtaaatt ccaaactatt atagcatgaa aaccacaaat tgagtaatat
      121 tttaattgat attactcgtt ttgtggttta aaatgtgctt attaagataa aaaccacaaa
      181 gtgaataatt tgaaaggagg cgctgaatga taactcttaa tgatcaattc attagaagct
      241 taagaagaca tagagccgat cttatactta ctaaaaacga tgcagcaaaa ttgataggta
      301 taaatcggaa aacatatgtc aaaatcgaaa atgggagcaa agaaagtatt agagcatcta
      361 cttatcaaaa gttggtaaat tggttactta atgatttgaa aatctaaata aatacaactt
      421 ggcgagttgg ctgattgaag aaatagaaaa tgagaggaga aaaataatgg atgcaggaat
      481 tacagtaatg ttagataaac aacaaacgaa tcaattgaaa aattatgttt ttgaaatgac
      541 aaaagaggct attgatcaag caagaattga taccggatta gaaagaccat ttctaaaagg
      601 aaaagagatg gctaaatatt tgaatgtatc atatacaact tttttgaaat ttaaaagaat
      661 gggattgcca gttattttat tagaaaaaat ggagttgttt tcaaaagaag aatgcaaaaa
      721 atggatattg agtcatcaaa tttaaaaaac acctcgtttt ttaaatttga tgaagtagaa
      781 aaaattaaaa agagtgttcc cgtcagtttg cagatcgggg gaacactcaa taataaagtt
      841 ctgagaaaac tttatttgaa tctattatag caaataaaat cagaagcagg tgtgacaatg
      901 aatgaaaaag aagttgaaag cttagttgaa aagcaaggct attctaattc tgatatatat
      961 gcagaattat a
//
LOCUS       DACEXQ010000383          967 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.402, whole genome shotgun sequence.
ACCESSION   DACEXQ010000383 DACEXQ010000000
VERSION     DACEXQ010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.402"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..492
                     /locus_tag="IXH04_002875"
     CDS             <1..492
                     /locus_tag="IXH04_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAQ1853396.1"
                     /translation="TLTGVSNRTIEKAITLSKSIFNGLIIAGKMHGAGVDEPIMNLDI
                     AKRFIKTGVDILLVPAPYTVPYFMESDLRGIVDYIKDFNRNKSIEEKVLVLTANGTSQ
                     DSSDQETIKKIALASKACGADIQHIGDSLNGICLPENIFALGQAIRGYRHQIIMLGKS
                     NYR"
     gene            511..>967
                     /locus_tag="IXH04_002876"
     CDS             511..>967
                     /locus_tag="IXH04_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1853397.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNIT"
BASE COUNT          330 a          137 c          175 g          325 t
ORIGIN      
        1 actctcacag gagtatctaa tagaactatt gagaaagcaa taacattatc caaatcaatt
       61 tttaatggat taattatcgc tggaaaaatg catggtgctg gtgttgatga accaattatg
      121 aacttagata tagcaaaaag atttattaaa acaggtgtag atattttatt agttcctgct
      181 ccatacactg ttccgtattt catggaaagt gatttgcgag gaatcgttga ttatataaaa
      241 gattttaata gaaataaatc tattgaggaa aaagttctag tactaacggc taatggaaca
      301 agccaagact cttctgacca agaaactatt aaaaaaattg cattagcatc aaaagcatgt
      361 ggagctgata ttcaacacat aggggattct ttaaatggta tttgtcttcc agaaaatatt
      421 tttgctttgg gtcaagcaat tcggggatat cgtcatcaaa ttattatgtt aggaaaatct
      481 aattatcgtt aattaaggag gaaatgaaag atgatcgaca aagttcaaaa aatagctgca
      541 aaaattagta atcaaagaca tctactagct ttaagagaca gcatggctgc agttatgcca
      601 ttaatcatta ttggttcctt ttttatgtta ttagcaaatt tcccgataga aggatttaca
      661 aaatggttat ctgaaattgg agtacaatcc tttttgaaca aagcatcaga ttcaacattt
      721 ggtattattg gattagctgt tacctactct ttgggacaca atctagcaaa gacttatggt
      781 gtagactcat tatcagcagg attattatct ttgagtggat atgttttact tacaccttta
      841 ttggatgcag atggttctat aggatttcca acaaaatact ttggtacctc gggtcttttt
      901 gttggtatta ttgtagcact tgtatctaca gaaattttcc ggtggtttgt acttaaaaat
      961 attacaa
//
LOCUS       DACEXQ010000384          964 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.403, whole genome shotgun sequence.
ACCESSION   DACEXQ010000384 DACEXQ010000000
VERSION     DACEXQ010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..964
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.403"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            32..>964
                     /locus_tag="IXH04_002877"
     CDS             32..>964
                     /locus_tag="IXH04_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HAQ1853398.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGH"
BASE COUNT          346 a          171 c          215 g          232 t
ORIGIN      
        1 caaataacca tgacccatta ggaggataat gatggctaaa gtaatagaaa aagaaaaaac
       61 gaagacaatt gacgtacaag caatgattga tgatttggca gaaaaagcaa atgcagcatt
      121 gaaagaaatg gaaagctttg atcaagaaaa agtagaccat atcgtgcatg aaatggcaat
      181 ggcagctttg gatcaacata tgccattagc gaagatggct gtagaggaaa caggccgcgg
      241 tgtttacgaa gataaagcaa tcaaaaatat gtatgcgtcc gaatatattt ggcataatat
      301 caaacatgat aaaacagttg gtgtgatcaa tgaagatgtc caaaaagggt tgattgaaat
      361 tgctgaacca gtcggtgtgg tttgcggagt aactccaaca acgaatccga cttccacaac
      421 gattttcaaa tcaatgatcg cgttgaaaac aagaaatcca atcgtttttg ctttccatcc
      481 aagtgcccaa aaatcttctg cagaagcagc acgagtggta cgtgatgctg ctatcgctgc
      541 cggagctcct gaaaattgta tccaatggat cgaacatcct tcgatcgaag cgacttctat
      601 gctaatgaac catccaggta tcgcgatcgt cttagcgaca ggtggagctg gaatggtcaa
      661 gtctgcttac tctacaggaa aaccagcttt aggtgtaggt ccaggtaacg taccagctta
      721 tattgaaaaa acagcaaaaa ttaaacgtgc agtgaacgat ttgatcgttt cgaaaacatt
      781 tgataatgga atgatttgtg cttctgaaca agctgtgatc gtggataaag aaatctatgc
      841 agcagtaaaa gcagaattcc aagcacacca agtttatatc gtaaaaccag atgaattgca
      901 aaaattagaa gatgcagtga tgaacgaagg aaaatatgcg gtcaatccat caatcgttgg
      961 acat
//
LOCUS       DACEXQ010000385          946 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.404, whole genome shotgun sequence.
ACCESSION   DACEXQ010000385 DACEXQ010000000
VERSION     DACEXQ010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..946
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.404"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..>946)
                     /locus_tag="IXH04_002878"
     rRNA            complement(<1..>946)
                     /locus_tag="IXH04_002878"
                     /product="16S ribosomal RNA"
BASE COUNT          201 a          299 c          210 g          236 t
ORIGIN      
        1 tgcccaactg aatgatggca actaacaata agggttgcgc tcgttgcggg acttaaccca
       61 acatctcacg acacgagctg acgacaacca tgcaccacct gtcactttgc ccccgaaggg
      121 gaagctctat ctctagagtg gtcaaaggat gtcaagacct ggtaaggttc ttcgcgttgc
      181 ttcgaattaa accacatgct ccaccgcttg tgcgggcccc cgtcaattcc tttgagtttc
      241 aaccttgcgg tcgtactccc caggcggagt gcttaatgcg ttagctgcag cactgaaggg
      301 cggaaaccct ccaacactta gcactcatcg tttacggcgt ggactaccag ggtatctaat
      361 cctgtttgct ccccacgctt tcgagcctca gcgtcagtta cagaccagag agccgccttc
      421 gccactggtg ttcctccata tatctacgca tttcaccgct acacatggaa ttccactctc
      481 ctcttctgca ctcaagtctc ccagtttcca atgaccctcc ccggttgagc cgggggcttt
      541 cacatcagac ttaagaaacc gcctgcgctc gctttacgcc caataaatcc ggacaacgct
      601 tgccacctac gtattaccgc ggctgctggc acgtagttag ccgtggcttt ctggttagat
      661 accgtcaagg gatgaacagt tactctcatc cttgttcttc tctaacaaca gagttttacg
      721 atccgaaaac cttcttcact cacgcggcgt tgctcggtca gactttcgtc cattgccgaa
      781 gattccctac tgctgcctcc cgtaggagtt tgggccgtgt ctcagtccca atgtggccga
      841 tcaccctctc aggtcggcta tgcatcgtgg ccttggtgag ccgttacctc accaactagc
      901 taatgcaccg cgggtccatc catcagcgac acccgaaagc gccttt
//
LOCUS       DACEXQ010000386          923 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.405, whole genome shotgun sequence.
ACCESSION   DACEXQ010000386 DACEXQ010000000
VERSION     DACEXQ010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.405"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..838)
                     /locus_tag="IXH04_002879"
     CDS             complement(<1..838)
                     /locus_tag="IXH04_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1853399.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGD"
BASE COUNT          332 a          185 c          148 g          258 t
ORIGIN      
        1 aatctcctgt ggatgtcagg ccattgtaga tatacgtatc caaaacttga gtcacatcat
       61 ataatgcacc ggaattccta ggaatgttat aaaacaggcc aaaatcagct cggaaaatgt
      121 ttcctacggc taaaatcgtt aaaatcgtca ttagtggaat aagttgtggt atcgtcacat
      181 gtttgatctg ctgccatttg ctagctccat caaccatagc agcttcataa taagtcggat
      241 cgattcccat aacagatgca aagtaaataa tgctgttata accaatccct ttccatgtac
      301 caatgaacac caagataaac ggccaatatt tcggttcatt ataccaattg acagcttctc
      361 ctccatgtcc agtgatccac tgattgaata ttcctttgtc aggacttaaa aaagcgtaaa
      421 caaagtaact gataatgacc caagatagaa aataaggtag taaggacatc gtttgataaa
      481 ctttaacgag tcgtttattt ctcaactcgc tcataatgat tgcaaatacg actgagatca
      541 gcagattcaa aaggataaat gctacattat ataaaattgt atttttagtg atgagaaatg
      601 cttgatccga agaaaataaa aatttgaaat tttcccaacc cacccaagga ctttccctca
      661 agctagccaa gaacccatct gctgaaatat gaaaattttt gaatgctacg acattcgcca
      721 ttacaggtat gtaaaagaaa aaaatcatcc aaagcatacc cggtacagcc atcaggacaa
      781 gtgctttata acgccataga ttcataaaaa aatgattgaa cccctttcgt tttttcatct
      841 aaaaacccct ttcgattcat tcgcaacgta attatagcgc tttcaataat gagaataaat
      901 aaacaaagta tagggaaaat gaa
//
LOCUS       DACEXQ010000387          915 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.406, whole genome shotgun sequence.
ACCESSION   DACEXQ010000387 DACEXQ010000000
VERSION     DACEXQ010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..915
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.406"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(749..>915)
                     /locus_tag="IXH04_002880"
     CDS             complement(749..>915)
                     /locus_tag="IXH04_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAQ1853400.1"
                     /translation="FDHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDG
                     RAYQMMGIEN"
BASE COUNT          294 a          144 c          138 g          339 t
ORIGIN      
        1 ttttttctct ttttattcta aggttatttt agtcttgtcc tttaatacaa agattttatt
       61 tttaaataaa aaatactctt tttttacgat tattgaaaaa aaaagataaa acttgattaa
      121 ttgaagtatt gcttaaattt ggaagctatg agggaagaat aaaagagtta ggacgggatt
      181 atctactatt tttctttgtt ttaaatattt gttatctttg aacataaaag agaaatgtgt
      241 cgactgcagt gttgtgtccc tcatcacgaa taaacggtgt tcaattagct gatcttgtat
      301 tcacttaaaa aatggtaaag tacgagttgc tattccccat tttcaatttt ctaacgctca
      361 ggacaaaata ctgttttcac aacctcattg gaataaaagc atatatattg tttcaagaac
      421 gaaatcttgt tatcttttta aagggcatct gtccaaaatc taattttagg aggtacgttt
      481 tggtatttat tcgtacaaat aaaagagaat tatctttatt catgtaacgc atccaattga
      541 ttttttcatg tacgctcctg gaacgatttt actgacgatt gcttatttaa ctgcgaatcg
      601 ctcattagct tttatcatag ctatccatat tttgaataac gttcttggct ttgtattata
      661 gatacagatg acaaaaaaag ttcaagtatg cggtgtagaa ccaatatcgc atacttgaac
      721 ttttttcaat gagtataaat taaaacttct aattttcaat tcccatcatc tgatatgccc
      781 gaccgtctga acttcttttg aaaaagccaa atgtgattag ataacgtctt tgttcgacat
      841 aatcgccact agaaaatacg ttcagcagta tcgaattaac ctctgtttct gtataagtca
      901 ctccatggtc aaagc
//
LOCUS       DACEXQ010000388          891 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.407, whole genome shotgun sequence.
ACCESSION   DACEXQ010000388 DACEXQ010000000
VERSION     DACEXQ010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.407"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            6..>891
                     /locus_tag="IXH04_002881"
     CDS             6..>891
                     /locus_tag="IXH04_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAQ1853401.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGETKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQIT"
BASE COUNT          303 a          120 c          162 g          306 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaaact aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgatga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgttggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact g
//
LOCUS       DACEXQ010000389          891 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.408, whole genome shotgun sequence.
ACCESSION   DACEXQ010000389 DACEXQ010000000
VERSION     DACEXQ010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.408"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            10..>891
                     /locus_tag="IXH04_002882"
     CDS             10..>891
                     /locus_tag="IXH04_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
                     /protein_id="HAQ1853402.1"
                     /translation="MTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIATVGVGDN
                     PLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRLNQMGIMS
                     IRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQVLPRDYT
                     RRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLNIGTSILETRPGFSHQMKIPITDNTK
                     ELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQINEENLDKIIDK
                     IRQKYGFTSIVHASSMLE"
BASE COUNT          320 a          144 c          194 g          233 t
ORIGIN      
        1 atcttaaaag tgacccgatc actgaatctt tttacgactg aaggaacacg aagtcaacgg
       61 agaaagaagc tggctcaaat gatccaggag aatattaaag aagagctagg attgattgct
      121 acagtaggtg tcggagataa tccccttttg gctaaactag ccttagacaa cgaagcaaag
      181 cataatgaag gatttgttgc ggaatggacg tacgaaaacg ttcctgaaaa agtatggaat
      241 attcctgaaa tgacggactt ttggggaatt ggctctcgaa tgaaaaaaag actcaatcag
      301 atggggatta tgagcattag agatcttgct aactggaatc cttatacgat taagaataga
      361 ctaggtgtga ttggcttaca gctgtacttc catgctaacg gaatcgatcg aacagatata
      421 gcaatcccgc cagaaccgac aaaggaaaaa tcttatggta attcacaagt gttaccacga
      481 gattatacta gaagaaatga aatcgagctg gtggtaaaag aaatggcgga acaagtagcc
      541 attcgtatca ggcagcataa ttgtaaaaca ggatgtgttc atttaaacat tggtacgtcg
      601 atcttagaaa cgagaccagg attttcacac cagatgaaaa tacctatcac agataataca
      661 aaggaattgc agaattattg tcttttcttg tttgataagt attatgaagg acaagaagta
      721 aggcacgttg gaataaccta tagcaagctt gtgtacaccg attcgcttca gctggattta
      781 tttagtgatc cacaaaagca aattaacgaa gaaaatttag ataaaattat tgacaagatt
      841 cggcaaaaat atggttttac gtcgattgta catgcttctt caatgttaga a
//
LOCUS       DACEXQ010000390          891 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.409, whole genome shotgun sequence.
ACCESSION   DACEXQ010000390 DACEXQ010000000
VERSION     DACEXQ010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.409"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..778
                     /gene="tnpB"
                     /locus_tag="IXH04_002883"
     CDS             <1..778
                     /gene="tnpB"
                     /locus_tag="IXH04_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAQ1853403.1"
                     /translation="DSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSSGKYYISLL
                     CKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQRKLSRRAL
                     LAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDIICIEDLNT
                     KGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICSECGHKDGK
                     KSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT          332 a          139 c          160 g          260 t
ORIGIN      
        1 tgatagtaaa ttcatcaaag ttcctaaatt gaagtcatta gtaagaatta agcttcatag
       61 acaaccaaaa gggattatca aatctgctac aatatcacgt cattctagcg gtaaatacta
      121 tatttctttg ttgtgtaaag aagaaattat cgaattacct aaaactaatt ccacaattgg
      181 tattgactta ggtattatgg actttgcgat tctttctgat ggacaaaaaa ttgataataa
      241 taaattcacg tccaaaatgg aaaagaaact aaagcgtgaa caacgcaaat tgtctaggcg
      301 tgctttattg gctaaaaata aaggtaccaa tctatttgaa gctaaaaatt atcaaaaaca
      361 aaaacgcaaa gtagctagat tgcatgaaaa agtaatgaat caacgtactg attttctgaa
      421 taagttgagt acagaaatga tcaaaaatca cgatattatc tgtattgaag acttaaacac
      481 aaaaggtatg ttgcgaaatc ataaattagc taaaagtatt tctgatgttt cttggtcaaa
      541 gtttgtgact aaattacaat acaaggctga ctggtatgga cgaaaaatca tcaaagtaga
      601 taaatggttt ccgtctagtc agatttgttc agagtgcgga cataaagatg gcaagaaatc
      661 tcttgaaatt cgagaatgga cttgtcctgt ttgtcatact catcatgatc gtgatatcaa
      721 tgctagtatc aatattctga ccgaaggtct aagactacat tctatgggat tggcttagat
      781 ccaatcatca agaaccgtag gaactacggg gatagcttgg taaataagag acaccgctgt
      841 tagtaaagaa atacgctatc aagtatgctc tattcccaag aagctcccac t
//
LOCUS       DACEXQ010000391          877 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.410, whole genome shotgun sequence.
ACCESSION   DACEXQ010000391 DACEXQ010000000
VERSION     DACEXQ010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.410"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            76..609
                     /locus_tag="IXH04_002884"
     CDS             76..609
                     /locus_tag="IXH04_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAQ1853404.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          275 a          164 c          166 g          272 t
ORIGIN      
        1 tctatgtttt ttgttaaatt tatttgcttt tttcaaaagt atgttacagt aatttcccaa
       61 ggaggtttta cagaaatgaa ttttgttgcc atggattttg aaactgccaa tcatcagcct
      121 tatagtgctt gttctcttgc gctagtcatg gtaaaaaata gccagatcgt tgatgaattc
      181 tatacattga tacagcctga gactcctttt ttctggcgaa atgtccaaat tcacggaatc
      241 catcaagaag acgtacgcaa tgcgccgaaa ttcccagaag tctggcaaac tatccaacca
      301 tattttcaaa aaaatcgttt agtcgttgca cataatgctg cttttgatac aaaggtctta
      361 gcgggatgct tagattatta tcatcttgaa cagcctaatt atctttcttt atgtacagtg
      421 aaaagtagcc gtcgcctgtt tcctgaattt ccaaatcatc gattgaacac tgtgtgcgaa
      481 aatttgaaca tccaattaga tcatcaccat gatgcattgg aagatagtcg tgcatgtgcg
      541 gaaatcctgc tttatcaaga aaaaaaattc gggacagatc cattgaaaaa acttgtgact
      601 ataaagtaaa agaatactta tgaaacacac aaagaggtcg tgagcctgcc gtttcactcc
      661 gagtcacaag gagcaatttc aaaaaatagc ttctcatgtt tattaaatat tgtgactggt
      721 gaccgaggag ctggcaggag aacaccgttt attggagagg ttgtgaaaaa gctgttctgc
      781 atcaagtaat aagaacagcc aaacaaaaat agcttctcat atttttcatt tggttggact
      841 tattaccgca gatgcaagct tttggacacc gtttacc
//
LOCUS       DACEXQ010000392          870 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.411, whole genome shotgun sequence.
ACCESSION   DACEXQ010000392 DACEXQ010000000
VERSION     DACEXQ010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..870
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.411"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..>870)
                     /locus_tag="IXH04_002885"
     CDS             complement(<1..>870)
                     /locus_tag="IXH04_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ1853405.1"
                     /translation="EKLTILELKRTRFLCHDCGRTFSAKTDLVDEHHQLTKELKQAIL
                     MELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVLCMDEFKSMNSVSG
                     SMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLVMDMNASYCQLLKT
                     VFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYRRLKRYWKLLLKDS
                     TTWEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLRYYFVKRGTEGFQE
                     TLKTISSTLPQSFK"
BASE COUNT          246 a          168 c          186 g          270 t
ORIGIN      
        1 tttaaacgac tgaggtaacg tagatgaaat tgttttaagg gtttcttgaa agccttccgt
       61 acctcgttta acgaaataat agcgtaataa ttgacaaaaa tgatacgcag ttcgcaattc
      121 ttcgttgtag cttaacagct catcaacgat ttctgtttga ctaattggtc gtttaaacag
      181 tcgatgataa tgccgtttga gtggctccca tgtcgtggaa tctttgagta agagtttcca
      241 atatcgcttg agccgacggt acttcttctg attttcagag tgtgaggttc gaaaacgatt
      301 catgatctgg acacgtaatt gattgaagct gcgattgatg tgttggacaa tatgaaagcg
      361 gtcagttacg atctccgcat ttggaaagac tgttttaagc aactgacaat agctggcatt
      421 catgtccatc actaggtact tgacctttaa acgggccttt ctaggaaagc gcaggaagta
      481 ttgagttagt ttgtaaagtc tgcgatcttc aaggatcgta aatagctgat tggttgtccc
      541 atcaacacaa ataaaactca tggaaccaga aacagaattc attgatttga actcatccat
      601 acacaacacc gtcggaagga agttcatacg tggctggtaa tgcttcgttg cttcacgcaa
      661 gatccgtgtc accgtcccat ccgagacgaa gtacttctta gcgattagtt tacgtgattg
      721 attttcgtac agttccatga gaatcgcctg cttcagttcc tttgttagtt gatgatgttc
      781 atcaactaag tccgttttcg cagaaaaggt ccttccacaa tcatggcaga gaaaacgggt
      841 acgttttaat tctaaaatag ttaacttttc
//
LOCUS       DACEXQ010000393          852 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.412, whole genome shotgun sequence.
ACCESSION   DACEXQ010000393 DACEXQ010000000
VERSION     DACEXQ010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.412"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            455..538
                     /locus_tag="IXH04_002886"
     tRNA            455..538
                     /locus_tag="IXH04_002886"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:489..491,aa:Leu,seq:cag)
BASE COUNT          350 a          112 c          161 g          229 t
ORIGIN      
        1 atttttattt attgtgtcat atcgtattaa gaatcatcga acaatacaaa tatgaattac
       61 aataaaaaaa ttatttgaag ttcgttcatt cgttacttat gatctctaga agaaagaaaa
      121 cgtgaattac ttcagcgtct agaattccag aaggatagta atcaacagaa gaaataatca
      181 atatccaaat gaaagaagat agctgaatcg agaaaaagag gaaaaaagaa atgaagaaaa
      241 aaataaaata gttactaatc ccagagtcgt tcgtttaaca ttgaaatcga tcatatggat
      301 aaaacagatt ttttcaccaa ccctcaatag atgatggagt tgctcggatg aattatttgt
      361 gtagatggca taagggaggg cgtacagaaa tgaaaaagaa caaaaatatg ttcttgacaa
      421 aaaacacatg taatcctaaa ctaaaagatg tgatgcggct atggcggaat tggcagacgc
      481 gctagcttca ggcgctagtg agggtgacct cgtggaagtt cgagtcttct tagccgcata
      541 atttgttttt ttataaagag aaggtacacc tggagcgatg agaaagaaaa taacatatta
      601 tctaaaagaa tataggaaaa attttaaaca acggaaccag attcaaataa atcaaaaaaa
      661 atttgtgtga aggtgggaca gtttaaaacc taatagattg attcaaatag tagatttata
      721 gattctgata caagaaatac tggcaaacga tagttaagga ataaagacta aacttaaact
      781 caaaaagagg tcatgcgaaa gaataaatcg catgacctct tactatattt tgaatgtgtt
      841 taaacgttca aa
//
LOCUS       DACEXQ010000394          845 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.413, whole genome shotgun sequence.
ACCESSION   DACEXQ010000394 DACEXQ010000000
VERSION     DACEXQ010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..845
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.413"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..674)
                     /locus_tag="IXH04_002887"
     CDS             complement(<1..674)
                     /locus_tag="IXH04_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="HAQ1853406.1"
                     /translation="MLNEKFLKYVKGWYFIYFGFILMLRAIGPYLLLPTRYDTLLFWP
                     AGIAGVVLIIIDAIIAFREKNLKKYDWLLIAFIGAMLVSSLMNLKYGYSENIKLIMWQ
                     LIFFFMIYQFGKDYSSNFFFKVFTWILSLVWFLANLVSLYWFVIQYGIKIPIKYKYYP
                     VRLGWLGNRLFGVYTDPNFGAVISLIVIIIAIYYLVTYKHLNLLFKIFFAINIFIQFS
                     FIALSGS"
BASE COUNT          339 a          140 c          100 g          266 t
ORIGIN      
        1 gagcccgata aagcaataaa cgagaattga ataaaaatat taattgcaaa aaatatttta
       61 aatagaagat ttaaatgctt gtaagtgacc aaataatata tagcaataat gatgacaatt
      121 aatgatataa ccgcaccaaa atttggatct gtatatacac caaataaacg gttgcctaac
      181 caccctaaac gaactgggta atacttatat tttattggaa tttttatgcc atattgaatg
      241 acaaaccagt ataatgatac taaattagct aaaaaccata ccaatgaaag tatccaagta
      301 aatactttga aaaaaaagtt actactgtag tctttgccaa actgataaat cataaagaaa
      361 aagattaatt gccacatgat taacttgata ttttcgctat atccatattt taaattcatt
      421 aaactgctta ctagcatcgc gccaataaaa gcaattaata gccaatcata ttttttaaga
      481 tttttttctc taaatgcaat aattgcatct ataatgatca agacaactcc cgctattcca
      541 gcaggccaaa aaagtaaagt atcatatcta gtaggtaata gtaaatacgg gcctattgct
      601 cgtaacatta gaataaaacc aaaataaata aaataccaac ctttcacata ttttaaaaat
      661 ttttcattca acatttttcc tccatccacc tatttctcat aaacatttct tatccttttt
      721 aggataaact ggttctttca attctaacaa aaaaatgcta aactaacaat gtatattaat
      781 gaatttcgag aggtgaaaga tgaaaagagt aattacttac ggtacctttg atttgcttca
      841 ctacg
//
LOCUS       DACEXQ010000395          839 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.414, whole genome shotgun sequence.
ACCESSION   DACEXQ010000395 DACEXQ010000000
VERSION     DACEXQ010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..839
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.414"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
BASE COUNT          288 a          153 c          110 g          288 t
ORIGIN      
        1 aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacagcgg aagcagaagc
       61 aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttc cgtgaatttc
      121 ttcttatttc ttggagttaa acgcttctgt tccagcctct ttttatctgt ctttcactaa
      181 tgatcagttg tagataagca agttgctttt tcttacatca taccgcccat caagacaaac
      241 gtttttagct tgtacgtact gggcaaaaat cccattatat caaggaaaaa caacattgat
      301 agtacttaaa attattaggc tagaacggac tagtaagagt tttttgcccc ttttttgccc
      361 cctctatcta caataaaaat attatccatt cttatatgac cctatataaa taataaaaag
      421 aggagaaaaa atatctcctc ttttttcata ttactacacc aacctaaaaa ggaggcgaag
      481 cgaggtttct acctcataag ttgaattaat actatcattc aacacagaat tcgtcaagaa
      541 tttttgttaa tgaattaaaa aaattcttaa tgtgcatatt ttgttcatgt agttctaaag
      601 ttttatttac tctttctaaa tagttagaaa aatctttatt tctgtgtgaa cgcatccctt
      661 ctgaacacat aattctatgt agttcaaaca aagcaaatat ccttgcaaca ttcaaatttg
      721 taatctcatc atgtgtaata ccaatccgtc tagcattagc atatacagaa ttttttatta
      781 tgcttttccg ctgtaaattc aataatatag gtttattatg agcacaagaa tttctaatg
//
LOCUS       DACEXQ010000396          826 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.415, whole genome shotgun sequence.
ACCESSION   DACEXQ010000396 DACEXQ010000000
VERSION     DACEXQ010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.415"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(232..305)
                     /locus_tag="IXH04_002888"
     tRNA            complement(232..305)
                     /locus_tag="IXH04_002888"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(269..271),aa:Ile,seq:cat)
     gene            complement(494..>826)
                     /locus_tag="IXH04_002889"
     CDS             complement(494..>826)
                     /locus_tag="IXH04_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1853407.1"
                     /translation="IDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRAD
                     GIYRSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKR
                     WYLNIPKR"
BASE COUNT          269 a          160 c          131 g          266 t
ORIGIN      
        1 aaactcttct ttattatcat ctcacaattt aacccaaatg tctttctatt taaaaatcaa
       61 agtaaaactt ttcaaatata cagaaatata actatgtgaa acatcctttc attaatccat
      121 aaaaggatac ataaaaaagc cactcatttg agtggcaatg aagaaaagct ttagcttgta
      181 taatactctt caaaaaattc taacacagaa cgattcaaat ggctacgtta atgtaccctg
      241 taggactcga acctacgacc ggacggttat gagccgtctg ctctgaccaa ctgagctaag
      301 ggtactggtt gttgccacat aaagccataa acaatcaacc agtagaatgt gtggcaacaa
      361 acctgttatc gcatagcttg gagtgtgact attatgggtg atagtgaaga tatgcgataa
      421 catcactatt ttatcgaatg atttttatag ttgtcaatat agttatgtaa tgctcctcaa
      481 cgaggagcta tttttatcgt ttaggaatat ttaaatacca acgtttgtca tggaaatctt
      541 gcgcaccgcc tttagtgttt ccctctggat cattcgttgc ccgcatcatg acatagactt
      601 tcttattagg gaagttacgc atgttaaaag atacatgata gccaacattt ccagaagtat
      661 tataagcttg atttacatct gatctataaa ttccatcagc tcttactcga gctaattctt
      721 tcccagtatt gtaatccata atgaagatat actcgtattt atagttagca atgtgccatc
      781 cagctacatg caaatttgca ttttcgattt ctccaaactg atcaat
//
LOCUS       DACEXQ010000397          811 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.416, whole genome shotgun sequence.
ACCESSION   DACEXQ010000397 DACEXQ010000000
VERSION     DACEXQ010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.416"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..473)
                     /locus_tag="IXH04_002890"
     CDS             complement(<1..473)
                     /locus_tag="IXH04_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1853408.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKS"
     gene            complement(484..690)
                     /locus_tag="IXH04_002891"
     CDS             complement(484..690)
                     /locus_tag="IXH04_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAQ1853409.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
BASE COUNT          223 a          181 c          130 g          277 t
ORIGIN      
        1 gatttgattc ccggttggtt ataaggagtg tccaacgtta atggaatacc atatttcatt
       61 gctgaatctc tagccaattc aacgtatgcc ttgtagtttt tctcaaacgt tgctttatca
      121 tgtgtgtgtt gtaactcaat ctgcacagga ctgttggcat tagcatacga accagcaccg
      181 tactgtacat aaccaggttg accgacttga taaacaattc cgccgtctcc cacaatataa
      241 gcagtataag cgctagtcca tgaacgttgc atatactgcg cttcattgcg tcctgttgct
      301 gtttcattag ccgtttcatg cagtaaaata tactgattat ttgctacttg tgagctacct
      361 tcatttgcgc ccaaattaaa ttcattgttg atagtatagg caaatccgtt aattggcaat
      421 aaaaaaagag ccgttaacag gctcatcgca gtaatagtaa ttttcttttt catatctatc
      481 ctcctattta tctgatgaaa ttttcttata cgttcgattt gatacaccta aaatactccc
      541 tagaaatgct ccaaaacctg taataatcat caccgttata tctgtgtacc gccagttaac
      601 accttttccg acaagaccga caaaagtcgc taaagcgggg ataactacta tagcgatcca
      661 tttcaaaata tcaaaggttc tattgttcat actatccacc tcccttcatt ggttgggcta
      721 ctttcttttt caatcgcttg ttttcttcta tcaatatttc tctttctgaa agcaaggcat
      781 caatttgccc gtccagttgt tcaattttat c
//
LOCUS       DACEXQ010000398          802 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.417, whole genome shotgun sequence.
ACCESSION   DACEXQ010000398 DACEXQ010000000
VERSION     DACEXQ010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.417"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(142..363)
                     /locus_tag="IXH04_002892"
     CDS             complement(142..363)
                     /locus_tag="IXH04_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HAQ1853410.1"
                     /translation="MGSSERIKIGNNVFIGMKSRILKGVHIGNNVVIGANSLVNKDVS
                     DDCVVAGNLAKVIMSLDEYYKKINNMKNP"
BASE COUNT          273 a          173 c           73 g          283 t
ORIGIN      
        1 ttataatccc ctactaaatt ttgcattgct cccaactctt cgacaaattg tcatctccat
       61 ctgtaaacag ccctaaaaac tcatataatg ctttttcatc aatatttttc ccatattatt
      121 ctcggtatag tttcacaagt tttacggatt cttcatattg tttatctttt tataatattc
      181 atctagggac ataattactt ttgctagatt tcctgcaaca acacaatcgt ctgatacgtc
      241 cttgtttact aaactatttg cacctataac tacattattt cctatatgta caccctttag
      301 tattctagac ttcattccta taaaaacatt attacctatt ttgatcctct ccgatgatcc
      361 caaagcttct ccatactact tttaaaaccg atcaatcata aacatgagtc aaaattgtaa
      421 ctccttcata tcatctccaa tttcaacaag agtcttcgtt ggaacaaaaa ctatacatcg
      481 ttcgcctatt cggcagccta aaaattgtag ttactttata tatcattctg atgtctcttc
      541 tcatcttttt accaataccg acgtaagaac tttcaaaata tacttaaact tcttattacg
      601 gatattaaac agaagaataa ctaaaatcga tatgacaacc gatataacta aatttatcac
      661 taaacttatc actttgaaat ttccgatatt aacatacgaa caagcaaaat aacttaaata
      721 agaagcaaca aaagctatta cacacaaact tatgagcttc caaacatatt tacctgccga
      781 ttcaaattta aatatatttt gg
//
LOCUS       DACEXQ010000399          801 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.418, whole genome shotgun sequence.
ACCESSION   DACEXQ010000399 DACEXQ010000000
VERSION     DACEXQ010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.418"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..>801
                     /locus_tag="IXH04_002893"
     CDS             <1..>801
                     /locus_tag="IXH04_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ1853411.1"
                     /translation="GNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLETT
                     IIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVIF
                     MDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAPTESAYIWKEVLQDLRKRGL
                     EEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIAHKVRVRGRKEICEDFKLVY
                     QANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTI"
BASE COUNT          271 a          137 c          157 g          236 t
ORIGIN      
        1 gggaattatt cacgtacatt taaaacagaa tatggcgagt tgaatttaac tattcctaga
       61 gatcggaatg gcgaattttc tcaacaaaca ctaccagcat ataagcgaac caatgattca
      121 ttagaaacga ccattatcca attatttcaa aaaggcatta ctatggctga aatctccaaa
      181 ttaattgaaa aaatgtatgg tcaccactat acgccacaaa caatctccaa catgagtaaa
      241 ttggtggctg aagatgtttt agcttttaaa gaaagaacgt tagaagccaa ttattccgtt
      301 atatttatgg atgcgacgca tatccctgtg aagcgacaga ctgtttcgaa ggaagcagtg
      361 tatattacga ttggtattcg tttggatgga accaaagaag ttcttgggtt tactattgcc
      421 ccaactgagt ctgcttatat ttggaaagaa gttcttcaag atcttagaaa acgtgggtta
      481 gaagaagttt tattagtagt gacagacgga ttaagctgta ttgaagaaag tatccatagt
      541 gtgtatccga atgcccaatt tcaacaatgt tgtgtgcatg tatctagaaa tatcgctcat
      601 aaagttcgtg ttcgaggtcg aaaagaaatt tgtgaggatt tcaaattggt ttaccaagcg
      661 aattcaaaag aagaggcatt ggatcacatc gactttatga ctaggaaatg gaaaaagcag
      721 tatccaagag tcgtcaattt actcttgaat cctgccctat taacctttta taatttccct
      781 cacgccatca gacgaacaat t
//
LOCUS       DACEXQ010000400          799 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.419, whole genome shotgun sequence.
ACCESSION   DACEXQ010000400 DACEXQ010000000
VERSION     DACEXQ010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..799
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.419"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..223
                     /locus_tag="IXH04_002894"
     CDS             <1..223
                     /locus_tag="IXH04_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAQ1853412.1"
                     /translation="RFETSLYEHVEIRNKNKVTADLFLLEVQKYHLNETVYDDKTGRI
                     DPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            239..445
                     /locus_tag="IXH04_002895"
     CDS             239..445
                     /locus_tag="IXH04_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853413.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
BASE COUNT          311 a          116 c          137 g          235 t
ORIGIN      
        1 tcgttttgaa acatccttat atgaacatgt cgagatacgg aataaaaata aagtaacagc
       61 tgatttattt ttgctagaag tacagaaata tcatttaaat gaaacagtct atgatgataa
      121 gacaggaagg atcgatccaa gaaaattgaa tgctgtaagc cgtttggcag gaaatgatta
      181 tgcagcaata ggagaaattt tcacgattgc acgtcccgaa taaaaaggag cactagttat
      241 gttaaaaata agctataaac catcgacaga ttcaaaagaa atgaaaaaag aatatgaaac
      301 agtgaatgat tttctgcaag ggcagtactt agaagttccg ccattgcaag accattttgt
      361 cgttactact gttactttag atggtaagga aatagaaatg ccagatcaaa cgattagcgg
      421 attgtttaat tactttaaca aataaatatt tattccgaaa agtgaaaaaa catataaaaa
      481 aacacataaa actaaaaaat tatcaaagaa tcctggtgca actgaaagaa tttgcaacgg
      541 gattctttca acttatttgt tatttttttc taaaaaatat taaagattgt taaatttcac
      601 tatttcattt caaattccat tgcctaatca ttaggaaaag tctatcatga actcgtaaat
      661 agaatatagt aataaccttg ttaggtgagg ctcctataca aacataggct gttgcccaga
      721 aacgtcgaaa gtcgccaatg ggtaaaacag aaactgttgg attaaggctt tttccaatag
      781 ctaagaatgt tttctttac
//
LOCUS       DACEXQ010000401          792 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.421, whole genome shotgun sequence.
ACCESSION   DACEXQ010000401 DACEXQ010000000
VERSION     DACEXQ010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.421"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            104..177
                     /locus_tag="IXH04_002896"
     tRNA            104..177
                     /locus_tag="IXH04_002896"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:138..140,aa:Ile,seq:cat)
     gene            221..>792
                     /locus_tag="IXH04_002897"
     CDS             221..>792
                     /locus_tag="IXH04_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HAQ1853414.1"
                     /translation="MSLKDDKPILRFNEQLEKLNDLGIYVADNEIHEAKNILSNHNYF
                     FKLLAYRKNYDKNEQKKYNISFLSIVDLAKLDMQLRYVLLPYCLDIEHSLKTYLMRLI
                     TNRKNKNGEYAENGYEIVKSVLADEDTPKDLKDQIFNGVRYRDSDGNLAFQEGFEKYY
                     NEPPIWVVLDLASISKIKFFVNYLANKQPN"
BASE COUNT          292 a          103 c          138 g          259 t
ORIGIN      
        1 tcactatcac tcataaatag tcacactcca agatatgcga taacaggttt gttgccacac
       61 cttctactgg ttgattgttt atggctttat gtggcaacaa cctgtactct tagctcagtt
      121 ggtcagagca gacggctcat aaccgtccgg tcgtaggttc gagtcctaca gggtacataa
      181 gaaatgttca atataaaggt ctaacaagga ggatattggt ttgagcttaa aagatgataa
      241 acctatactt agatttaatg aacaactaga aaagctaaat gatttaggaa tctatgtagc
      301 agataatgaa attcatgaag caaaaaacat tttgagtaat cacaattatt ttttcaagct
      361 attagcttat agaaaaaatt atgataaaaa tgagcaaaaa aaatataata tttctttttt
      421 aagtatagta gatttggcta aactagatat gcaacttcgt tatgtgttgt taccttattg
      481 tttggatatt gaacactctt taaaaacata tcttatgaga ttaattacta atcgaaaaaa
      541 taagaacggt gaatatgcag aaaatggtta tgaaattgta aaaagtgttt tagcagatga
      601 agatactcca aaagatttga aagatcaaat atttaatgga gtgagatata gagattctga
      661 cggtaatctt gcttttcaag aaggatttga aaaatattat aatgaacctc ctatctgggt
      721 agttctagat ttagcttcta ttagtaaaat taagtttttt gtaaattatt tggcaaataa
      781 acagcctaat aa
//
LOCUS       DACEXQ010000402          789 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.422, whole genome shotgun sequence.
ACCESSION   DACEXQ010000402 DACEXQ010000000
VERSION     DACEXQ010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.422"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..782
                     /locus_tag="IXH04_002898"
     CDS             <1..782
                     /locus_tag="IXH04_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1853415.1"
                     /translation="DMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNG
                     VSLARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGE
                     LISLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKL
                     IKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNIDKKVSSHMLRHSHISLLTELGI
                     PLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT          340 a          106 c          102 g          241 t
ORIGIN      
        1 aagatatgct agattactac tattttgaaa aaaatttatc atataattat gtaatacatt
       61 taaaaacttt tacaggaatg atttttaaat tcgccaaccg tcgatatgga ttaaaatata
      121 atcccgtaaa tggggtttct ttagcaagaa aacctaagac aaacgaagaa ataaaaaaag
      181 aaaaagatgg ttatttatca aaagaagaag ttcaaatgat tgctagtaaa caaaaaagtt
      241 cacatcaaca gtcacgttat tctttaatta ctaaattttt gtttttaact ggactacgtt
      301 atggtgaatt gatatctttg actgaatctg attatgatgg taataaaatt acggtttctg
      361 gtacttatga ttacgagtta aaaattaaaa caacaactaa aaatacaggt tcttatagaa
      421 ccattgaatt atcaagtaat gctaaagaaa tcatagatca attaattgaa gaaaacaaat
      481 taattaagaa taataaagat aaatacattt tcatctctaa aaatggtaac ccgatatcta
      541 ttcaagcata taatcaatct ttaagagcag tatccaaaga attaaacata gataaaaaag
      601 tttcttctca catgttaagg cattcccaca tttctctact tacagaatta ggaatcccat
      661 taaaagccat tatggatcgt gtaggacatg aagattcaaa aacaacatta aagatttata
      721 ctcatacaac taaaaatatg caaaatcaac tagtagaaaa actaggaaaa atagaaattt
      781 aaattgccc
//
LOCUS       DACEXQ010000403          781 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.423, whole genome shotgun sequence.
ACCESSION   DACEXQ010000403 DACEXQ010000000
VERSION     DACEXQ010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.423"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            123..>781
                     /locus_tag="IXH04_002899"
     CDS             123..>781
                     /locus_tag="IXH04_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853416.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PT"
BASE COUNT          256 a          152 c          133 g          240 t
ORIGIN      
        1 acactttttc tgacatttat caaagaagat acactctatt tatattcata gacaaggttt
       61 tacggattca aaaattcagg attatgctat actggtatta aaaaaataat gaggtatttt
      121 atatgaaaaa gttaggtttt attttattgt tggtctcttt attccttgta ggatgtacgt
      181 ctcctagtaa agaaaatagt gatccatctg cgaccacaga caaaagttca atcagacaaa
      241 ccagttcagc tgaaaaatca agtactcaga aatcagcttc tactgaaacg aatacatctt
      301 caacaagcag taaagtctca gagcccaata cttcttctac atctagtagt acagttgaga
      361 caaatatttc ttcttcaaca atagacacaa cctcctcaca gtcttctcag tcaatagatc
      421 cgttagcagg atattctgat ctacaaattg agtacgcacg tgtttggcta gctattagag
      481 gtaatacttt tagagataca tcgggtgatt tcgaattaca tgttattcat tatcctgcag
      541 gaacacctat cgccccttat gatgcaggga gtgcagtcta tccgaaagat gtggttatgc
      601 tcactgggaa atacagttac cagggagttt tagtgtattc aagtaaccat gacgggacaa
      661 tcactagtta tccagttcct tcacattggc aaatgcctgc agatcaaaca tcttatccag
      721 cgttcattag agaaacaact caagaaatca ttgataatgc atctgtagta gctattccta
      781 c
//
LOCUS       DACEXQ010000404          744 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.424, whole genome shotgun sequence.
ACCESSION   DACEXQ010000404 DACEXQ010000000
VERSION     DACEXQ010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.424"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(316..516)
                     /locus_tag="IXH04_002900"
     CDS             complement(316..516)
                     /locus_tag="IXH04_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ1853417.1"
                     /translation="MKINYTVDLVSFSLVINFCLFSLRLFIEGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
BASE COUNT          267 a          119 c          127 g          231 t
ORIGIN      
        1 aatctttgat aaatctattc gaagggtcaa atttaaacca taattgtgtt tttccttcca
       61 tcagcataaa agttcacctc aaaaagagta tacgaacgaa tgttcttttt gtgaaggcgg
      121 aatttaatat actgaataat agaaaacaag tacttgtgcc aaattatgtg ccaaaaaaat
      181 tcgaattcaa caaaatgtaa acaaatagaa aaccttctat attaagattt cctgcaataa
      241 caaacacaac agaagacgtg taatagttag tcaggaacgt acaaataacc cctgtatcct
      301 ttgcggtaca gggggttatt ttgtataagt agacattgta gtttctaaac tgctatgtcc
      361 taaacgtttc gatacgctaa gtatatttac tccttgataa agtaaaatag atgcatgcgt
      421 atgtcttagt ccctcaataa acagtcgaag agaaaatagg caaaaattga ttactaaaga
      481 aaaacttact aggtcgacgg tgtagttgat tttcatactt caacagattc gaattaaacg
      541 atttcgattg gcttatacca gtcctatccg acgaaagata tatcgttctt aactctagcg
      601 gtataacttg aagctttacg taaagccaat gggaatgaac tggagatgac tttttattat
      661 ttttagtaat agctgatggg ttaatactta caactttcct tttcaaagta ccatataaaa
      721 aaacggtggg gttgtttagc gaaa
//
LOCUS       DACEXQ010000405          736 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.425, whole genome shotgun sequence.
ACCESSION   DACEXQ010000405 DACEXQ010000000
VERSION     DACEXQ010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.425"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..197)
                     /locus_tag="IXH04_002901"
     CDS             complement(<1..197)
                     /locus_tag="IXH04_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HAQ1853418.1"
                     /translation="MNTRHRRVAKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASDAIRKFGISILNDDRKWEE"
     gene            complement(204..434)
                     /locus_tag="IXH04_002902"
     CDS             complement(204..434)
                     /locus_tag="IXH04_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853419.1"
                     /translation="MKYEIPLSEAGIQAIINGREVNIELPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSIDNTFRLGS"
     gene            complement(431..>736)
                     /locus_tag="IXH04_002903"
     CDS             complement(431..>736)
                     /locus_tag="IXH04_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853420.1"
                     /translation="ELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSNDE
                     LFKDSVGDVIVTLIILSMQKGTNVQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDVK"
BASE COUNT          179 a          180 c          103 g          274 t
ORIGIN      
        1 atttcctccc atttacgatc atcatttaat atcgaaatcc caaacttacg aatagcatca
       61 cttgcatcag caacaaactg acttgccact ttatatgttt cttctgctga aattccatat
      121 tctttttcaa actttgtctt tagtacattt agttcctgtt ttcttagttt tgctactctg
      181 cggtgtctgg tgttcattgt caatcaactc cctaatctga aagtgttgtc tatacttgat
      241 cgaaattctt ttaagtggtt ctctaccaca gaatcagtca cgttaaaacg atcaattaat
      301 actggagctg ccatatcttt caaataactt tgtctgatga ctaattcagt accatcagga
      361 agttctatgt taacctcccg accattgata attgcttgaa tgcccgcttc acttagtggt
      421 atttcgtatt tcatttcaca tcctccaaat cactcgactt cacgaataca ccatctacca
      481 ttttccctgt gcgtcctttg atttcgttgt atgccatttc taaacactct tgtacgtttg
      541 tccctttttg catggaaagg ataatcagcg tgacgataac gtctcctacg ctatctttaa
      601 atagttcatc attacttctt gccatcgccg aagctatttc cccgaattcc tcagctactt
      661 tcaaaaactg tgcttttgga tctgcttgat ccagtccctt gtctttagcc cactgctcta
      721 cttttgtgat tagttc
//
LOCUS       DACEXQ010000406          731 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.426, whole genome shotgun sequence.
ACCESSION   DACEXQ010000406 DACEXQ010000000
VERSION     DACEXQ010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.426"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(138..491)
                     /gene="tnpB"
                     /locus_tag="IXH04_002904"
     CDS             complement(138..491)
                     /gene="tnpB"
                     /locus_tag="IXH04_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAQ1853421.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(481..675)
                     /locus_tag="IXH04_002905"
     CDS             complement(481..675)
                     /locus_tag="IXH04_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853422.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          220 a          156 c          122 g          233 t
ORIGIN      
        1 ttgaagcaac tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa
       61 ctgattttgt tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt
      121 tgtgttaacc ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga
      181 tcgatactta atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg
      241 tctttcgtcc gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa
      301 ccatcaccgt cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa
      361 acggcatctt cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca
      421 attccttttc gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa
      481 tcataaagca ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc
      541 gttatagatc aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc
      601 cgcgggttta cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaatctc
      661 atttaattta tacatagaaa acctccagac ttcttgatag ctcaagtata tcttgaggtg
      721 catttgttag t
//
LOCUS       DACEXQ010000407          711 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.427, whole genome shotgun sequence.
ACCESSION   DACEXQ010000407 DACEXQ010000000
VERSION     DACEXQ010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.427"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            51..704
                     /locus_tag="IXH04_002906"
     CDS             51..704
                     /locus_tag="IXH04_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1853423.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
BASE COUNT          297 a           59 c          132 g          223 t
ORIGIN      
        1 ccataatatg ctgtataata tctagtgtat aggcttaagt ggaggtatat atgaaaatat
       61 taactgttga agatgataat atgataagag agggaataag tgaatatctt tcagaatttg
      121 gatatactgt tattcaagct aaagatggaa gagaagcctt gtcaaaattt aatagcgata
      181 taaatttggt tatcttagac attcagatac cttttataaa tggtttagaa gtgttgaaag
      241 aaattagaaa gaaaagcaat ttaccaattc taatcttgac tgcatttagt gatgaagaat
      301 ataaaattga tgcatttact aatttagtag acggatatgt agaaaagcca ttttcattgc
      361 cggtcttaaa agctagaata gattctttaa ttaagaagaa ttatggacat ttagagaagt
      421 ttgaatataa ggatctatcg gttaatttta atagctatac agctaaaata aatggtgaag
      481 aaatagatgt aaatgcaaaa gaactagaag tattaaaatg tttattggat aataatggac
      541 aagtgttaac aagaatgcaa attattgatt atgtatggaa agatagcgaa gaaactcctt
      601 atgatcgagt aatagatgta tatataaaag aacttagaaa aaaattacaa ttagactgta
      661 taactactat aagaaatgta ggatataaat tggagagaaa atgaaaaaat t
//
LOCUS       DACEXQ010000408          706 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.428, whole genome shotgun sequence.
ACCESSION   DACEXQ010000408 DACEXQ010000000
VERSION     DACEXQ010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.428"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..>706)
                     /locus_tag="IXH04_002907"
     CDS             complement(<1..>706)
                     /locus_tag="IXH04_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853424.1"
                     /translation="WQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKANFLEFFGGKEG
                     SILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQSVLVGVYPFIS
                     SSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAAKDWLKTNIDK
                     QKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSARIVEYTFNVL
                     TNSYDKLVVGNVKTNF"
BASE COUNT          211 a          147 c          104 g          244 t
ORIGIN      
        1 aagaagtttg ttttgacgtt tcctacaact aatttatcgt atgaattggt tagaacatta
       61 aacgtatatt ccacaattct cgctgacata ttcactttta actgtggatg atacacatct
      121 actccgtcac ccatcgaaac tttttctaga tcaacaaatt tttcatagcc tctttgatgc
      181 ctcaatggta ctaattcaat cgaaccactc acttgtggtt tttgtttatc tatgtttgtt
      241 ttcaaccagt ctttagcagc ttcccttaat gtggctacat cagtcgcttt gtctttaaaa
      301 tcaacaaaag aaacatatcc agcaggataa tcatccacgt aatccgtgaa aataacttct
      361 tctggtagag tgatctcgtc ttctccttct gaagagctgc taatgaatgg ataaactcca
      421 actaaaacac tttgagcatc aatctctaag tcaagaccag ttaagttttt agtataaatc
      481 gctttgattt tatgatccgt acctagactt ttttcatgac gtaatgtgtt attatctttt
      541 agaaactcac catgaaatcg atctagaata gatccctctt ttccaccaaa gaattctaaa
      601 aaattcgcct tttctatctt cacattagca agcgtatcta ctaatgacga gaaagaaaac
      661 tgcgaaggga tagctggttt cgctaaaact tttgcgtttt gccatg
//
LOCUS       DACEXQ010000409          679 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.429, whole genome shotgun sequence.
ACCESSION   DACEXQ010000409 DACEXQ010000000
VERSION     DACEXQ010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.429"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
BASE COUNT          241 a          113 c           80 g          245 t
ORIGIN      
        1 caaattactt catcaaaaca ttgatataat ttttattata tcagtattta ttcttgatac
       61 ataaatgaga aaataaatac tttaaatcta ttgactacaa gtagtcatta agtcttcata
      121 agatgtacaa tatgacatta aaaaaataaa agcttacgac catccgttaa atctgacact
      181 aaataatgga ctgctttctt ttgtattagc aggttacgtt gactatgaat attcaccaga
      241 ttatttggag aaacctcttc aacacgagaa caatgatatt aattttagat ttgtctacga
      301 gtcaaaaagt aaaacatggt tagttgatca attaagcacg ataaacacaa gcttccatga
      361 attacctaaa cctgaaaatg aagttgagat ggtcattaaa atcttctaaa aaagaagcgt
      421 tgaaggggaa atattccttt caacgctctt ttttatatgc ttagaaacat aaataaattg
      481 tatactttct ttttagcccc tattactttt cacgaaaaat atcttttttt cgacgctagc
      541 gtttctctac ttctaatact gtacacagtt tttgtattat tgaaaaatta ctcattattt
      601 agcattaatc cacatcgttt tatcaatcga ccttcatact aaactacttt aaagtcatct
      661 tccttctcgt ttttcatta
//
LOCUS       DACEXQ010000410          679 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.430, whole genome shotgun sequence.
ACCESSION   DACEXQ010000410 DACEXQ010000000
VERSION     DACEXQ010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.430"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..>679
                     /locus_tag="IXH04_002908"
     CDS             <1..>679
                     /locus_tag="IXH04_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ1853425.1"
                     /translation="LLASILGQIVLNLMCAVVSQKMYPDYTPVGELEKETIDDIIKKV
                     KGLVTNKVGGTILRSSDSIIISSFLGLSVLAIYQNYYFIISAIMSIFAIIYESCIAGI
                     GNSLIVESQEKNYIDFCDITTIIGWFLAISCACFITLFQPFMIIWVGSDNLMSISFVI
                     FMIVHFFLYEIDQLIGLYKDAAGIWYADRFRPLITAMINIVLNIILVNWFGLYGVLAA
                     TIVSVLLV"
BASE COUNT          208 a           79 c          126 g          266 t
ORIGIN      
        1 ttattggctt ctattttggg acaaatagtt cttaacttaa tgtgtgctgt tgtttctcaa
       61 aagatgtatc ctgattatac tcctgttggt gagttagaga aagaaactat tgacgatatt
      121 ataaaaaaag taaaaggatt agttacaaac aaggtaggcg gtacaatatt acgatcttca
      181 gattcaatta ttatttcatc tttcttaggt ctttcagtac ttgcgattta tcagaattat
      241 tactttatta tttctgccat aatgagtata tttgcaataa tttatgaatc atgtatagca
      301 ggtataggta acagtcttat tgtagaaagt caagaaaaaa attatataga tttttgtgat
      361 attacaacaa ttataggttg gtttctggca attagttgtg catgctttat tacattattt
      421 cagccgttca tgattatctg ggtgggaagt gataatctta tgagtatttc atttgttata
      481 tttatgatag ttcatttttt cttatatgaa attgatcagt taattgggtt atacaaagat
      541 gctgctggaa tatggtacgc agacaggttt agaccattaa ttacagcaat gataaacatt
      601 gtattaaata tcatcctcgt taattggttc ggattatatg gagttttggc ggcaaccatt
      661 gtttcagtat tgttggttg
//
LOCUS       DACEXQ010000411          678 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.431, whole genome shotgun sequence.
ACCESSION   DACEXQ010000411 DACEXQ010000000
VERSION     DACEXQ010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.431"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            18..206
                     /locus_tag="IXH04_002909"
     CDS             18..206
                     /locus_tag="IXH04_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14 like
                     family protein"
                     /protein_id="HAQ1853426.1"
                     /translation="MIPICLITKMMGKKLIYIESFAKVTSPTQTGKLMYHFADQFYVQ
                     WETMLQFYPNAIYLGGIY"
     gene            223..>678
                     /locus_tag="IXH04_002910"
     CDS             223..>678
                     /locus_tag="IXH04_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta(1,3)galactosyltransferase EpsH"
                     /protein_id="HAQ1853427.1"
                     /translation="MIFITLGSQKFQFDRLLKAIDDLIEKGVIIESTFAQSGYSDYKP
                     LNYSYKAFLNRDEFTETMQKAEMVITHGGTGAIIGAVKKGKRVIAVPRLAKYGEHVDD
                     HQLQLIRQFDEMNIIYPCYELENLEDAVKETRVRMFNKYVSNTETIINSI"
BASE COUNT          250 a           82 c          131 g          215 t
ORIGIN      
        1 gtacaggtgt tttagctatg atccctatct gcttaataac aaaaatgatg ggaaaaaaac
       61 tgatttatat tgaatcgttt gcaaaagtaa ccagccctac tcagacagga aaattgatgt
      121 atcattttgc agatcaattt tatgttcagt gggaaactat gctacaattt tatcctaatg
      181 ctatttattt gggtgggata tattaaataa taggagaaaa aaatgatatt tataacatta
      241 ggttcacaaa aatttcaatt tgatagattg ttaaaagcta ttgatgattt aattgaaaaa
      301 ggagtaataa tcgaaagtac atttgcacag agtggttata gtgattataa accactaaat
      361 tattcctata aagcatttct aaatagggat gagtttactg aaactatgca aaaagcagaa
      421 atggtgatta ctcatggagg tacaggtgct ataattgggg ccgtaaaaaa aggtaaaagg
      481 gtcattgctg ttcctcgatt ggctaagtat ggtgagcatg tcgatgatca ccaactgcag
      541 ttaattagac aatttgatga gatgaatatt atctatcctt gttatgaatt agaaaattta
      601 gaagatgccg ttaaagaaac aagagttcgg atgtttaaca agtatgtatc taacacggaa
      661 acgattatta attcgata
//
LOCUS       DACEXQ010000412          650 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.432, whole genome shotgun sequence.
ACCESSION   DACEXQ010000412 DACEXQ010000000
VERSION     DACEXQ010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.432"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(92..505)
                     /locus_tag="IXH04_002911"
     CDS             complement(92..505)
                     /locus_tag="IXH04_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HAQ1853428.1"
                     /translation="MDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVLSASIPI
                     LIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEKLLYITK
                     TSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
BASE COUNT          223 a           85 c           72 g          270 t
ORIGIN      
        1 actttacatt tttttaggaa tgtaaagtct tatctctttt cttaattgta caattagtat
       61 tatctgcact actttctaat taaataaatt tttagttatt aacttgatta aaatcagaga
      121 taatatcatt taattcagtt tgaagggttg taacgattaa atggaaattt tcttcatctg
      181 ttttattata tggggatgtt ttcgttatat ataacaactt ttctttttca attttcatta
      241 ataactctgt cgaagtctgt actttatctt gataattaaa tgcagacata ccactttgta
      301 taattgtaat tattgctgaa gcaattgaaa caatcaatag taaagacctg tgttcggaag
      361 cctgatcaat taatattggg atactagctg ataagactat ttttataata tttccgatac
      421 caattactct attataaaat cgaatttctt tttttaatct atcgattgta gaatcaatag
      481 attcgataaa cgctttttga tccatcattt tctctccaat cattttatta aaatcattaa
      541 caaaaatatt ataataggat ttaagtaagt tctaactaat cacatataaa aataaaaaaa
      601 catttgtatt ttatcaaagt tttattatac ttgtcaattt gtgtacatcc
//
LOCUS       DACEXQ010000413          648 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.433, whole genome shotgun sequence.
ACCESSION   DACEXQ010000413 DACEXQ010000000
VERSION     DACEXQ010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..648
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.433"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(320..622)
                     /locus_tag="IXH04_002912"
     CDS             complement(320..622)
                     /locus_tag="IXH04_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853429.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
BASE COUNT          229 a          111 c           90 g          218 t
ORIGIN      
        1 gggttatatt gtgagataat aataaagaag agtttaaagc gttccccaaa aaccacttcc
       61 ccataagtgt gttacgcttt aaactctttt atatttgaag ccattaaaaa gcataccata
      121 taactgtaaa aaataatggg aaaaagactt ataattggag tggtagttaa ttagtgactt
      181 atttttgatt ttatagcact gatactataa aatatagata tcatcatatt acacaatctt
      241 aatactaact taaaaaatat cccttttata agtatggtga taaaatccgt tccgggctac
      301 cttttttagg tagcctactt taatctttat acctttctgg atcaacgaaa gtatacttca
      361 aatagtcata acgccgatga tcgctccgtg cgtctggcac atcagtcacg atatcaaaca
      421 aaaaatacac atctttcttc attctggttt ttgttgccgg aattttaaag tagttcttat
      481 tagagtaata gagattgatt aataagctat cttcgattgc taaaaagaaa acttctgaat
      541 cccacacctt ataaaaatct ttgacaaatc tattcgaagg atcaaattta aaccataatt
      601 gtgtttttcc ttccatcaac ataatgttca cctcaaaaag agtatacg
//
LOCUS       DACEXQ010000414          646 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.434, whole genome shotgun sequence.
ACCESSION   DACEXQ010000414 DACEXQ010000000
VERSION     DACEXQ010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.434"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..>646
                     /locus_tag="IXH04_002913"
     CDS             <1..>646
                     /locus_tag="IXH04_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAQ1853430.1"
                     /translation="ENIYLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVR
                     KDKKLEPQREFIVQIGILDDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQE
                     RNPNLAVYNAVIHNDEISPHLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNG
                     RVLFNNFRNQEVDSIERLMNDYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAV"
BASE COUNT          243 a           92 c          141 g          170 t
ORIGIN      
        1 gaaaatattt atttagagca gacgaatcta gcagataaat atgaggaatt gtttggcgaa
       61 gtggtagaaa aatataatgc gaaacaaaaa agagccgata gaaaaatcga ggactattta
      121 gcgaaagtgc gcaaggataa aaagttggaa cctcaacgag aatttattgt gcaaataggc
      181 attttagatg atttccagac aaatcgagca gatggttctt ctactggcat atcgaaacaa
      241 caagctgaac aaaatcgagt gattgccaat aaaattttgg ttcagtatta caaggagttt
      301 caagagagaa accctaattt ggctgtttat aatgctgtta ttcataatga cgaaataagt
      361 ccgcacctgc acttgaatat tgtgccggtt gctgaaggtt ataaacgggg tgttcaaaag
      421 cagccgagct ttaataaagc actggttcaa caagggatac acgctgataa agataatggt
      481 cgagtgcttt tcaataattt tagaaatcaa gaagttgact ctatagaacg attaatgaat
      541 gattacggtt ggaaaaggga tattgtcggt accaacaaaa ttaaagatat tcacgaatac
      601 aaagaaatca tgagtgaaat ttctgaattg cgccaaacgg ctgtaa
//
LOCUS       DACEXQ010000415          645 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.435, whole genome shotgun sequence.
ACCESSION   DACEXQ010000415 DACEXQ010000000
VERSION     DACEXQ010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.435"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            64..137
                     /locus_tag="IXH04_002914"
     tRNA            64..137
                     /locus_tag="IXH04_002914"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:98..100,aa:Met,seq:cat)
     gene            156..228
                     /locus_tag="IXH04_002915"
     tRNA            156..228
                     /locus_tag="IXH04_002915"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:189..191,aa:Asp,seq:gtc)
     gene            232..304
                     /locus_tag="IXH04_002916"
     tRNA            232..304
                     /locus_tag="IXH04_002916"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:265..267,aa:Phe,seq:gaa)
     gene            309..379
                     /locus_tag="IXH04_002917"
     tRNA            309..379
                     /locus_tag="IXH04_002917"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:341..343,aa:Gly,seq:tcc)
     gene            392..465
                     /locus_tag="IXH04_002918"
     tRNA            392..465
                     /locus_tag="IXH04_002918"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:426..428,aa:Ile,seq:gat)
     gene            477..548
                     /locus_tag="IXH04_002919"
     tRNA            477..548
                     /locus_tag="IXH04_002919"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:510..512,aa:Glu,seq:ttc)
BASE COUNT          140 a          144 c          168 g          193 t
ORIGIN      
        1 aaaccgtcag gcgggtaaaa ccgtgcaagg gttcgaatcc cttatcctcc tttttcatat
       61 tatcgcggga tggagcagtc tggtagctcg tcgggctcat aacccgaagg tcgtaggttc
      121 aaatcctgct cccgcaatta aaaactaaaa acattggttc cgtggtgtag gggttaacat
      181 gcctgcctgt cacgcaggag atcgcgggtt cgattcccgt cggaaccgtt tggctcggta
      241 gctcagttgg tagagcaatg gattgaagct ccatgtgtcg gcagttcgat tctgtcccgc
      301 gccattatgc gggtgtagtt tagtggtaaa accacagcct tccaagctgt tgtcgcgagt
      361 tcgattctcg tcacccgctt ttagttttta tgggcctata gctcagctgg ttagagcgca
      421 cgcctgataa gcgtgaggtc gatggttcga gtccatttag gcccatttat tttattggcc
      481 cgttggtcaa gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt
      541 acgggtcatg gaagatactt tttgagtatc tttttctttt tatctgaaag aatgatttta
      601 aaaagcttag cagagttatc tttatggatg attgtgctaa gcttt
//
LOCUS       DACEXQ010000416          645 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.436, whole genome shotgun sequence.
ACCESSION   DACEXQ010000416 DACEXQ010000000
VERSION     DACEXQ010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.436"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            124..363
                     /locus_tag="IXH04_002920"
     CDS             124..363
                     /locus_tag="IXH04_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853431.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT          212 a          118 c          101 g          214 t
ORIGIN      
        1 cctctccata tatgtattac taagactaat agatactata aaagacttga tagttttgca
       61 ttgattttgc ctaatactga gcataggtgt atgctaaacg atactataag gagtgtgtac
      121 ataatgaaaa gaattctgtt tatcagttta tcaaatttat tgttacttac tgcttgtggg
      181 acttctgcat caaagaacat accttcagat acgcaaattt cgaatagtat cgattcaaca
      241 atttctacaa atgagaaaaa ttctccggta gaaacttccg atataaaagt ttctacctct
      301 gcaaaaaaca gggtaaagaa actgtcagca cgtctccttt atttcaaaca aactctgaat
      361 tagcttcttc atcttcttca gcaaataatg atgatagttt cgcgtttact cctaactaaa
      421 tgcaaccttt acaaatgctt tacgataaca agcctgagct aaatgatcct gatatcagtt
      481 ttgcattttt tgaaatgatt ggatcggatg atctttttaa aactactagt ttgtctatcc
      541 gaaaatagga cagttctggc agtataggtt tttatcgagt ttctccacca ggcctcgtta
      601 cagagacaga ttctctaggc gaaccttttg aataaatcaa aaatc
//
LOCUS       DACEXQ010000417          635 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.437, whole genome shotgun sequence.
ACCESSION   DACEXQ010000417 DACEXQ010000000
VERSION     DACEXQ010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.437"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
BASE COUNT          241 a           80 c           78 g          236 t
ORIGIN      
        1 tctgtgtaga tatttcgtta tatcactaaa gataataaga tttaatgacc tcattaatat
       61 tgtttgctag gtccaaactc ttattaaact ttccttgcta taaaacccaa acatagtaat
      121 aaaagaataa cttattacct ttaaattata ctaccaaaaa atggtagtat attattaatt
      181 tatataatat gaaacttaaa atatagtagt atgttaattt atactaaatt aaataaagta
      241 ctacctttaa taggtagtac tttattatca agccattatt ccttcttcta ctagttcttt
      301 ttgtcacata attttgcaat caataaatgt tgcgcaataa gtataagaat tccattttct
      361 aaatttgaaa ggtgatccat aatttcatgt tcaagtagtt tattaattgt ccacttgtcg
      421 cttcatctaa aaattttata ggttttagga atgctattcg cttattttag gaagatttta
      481 actgtttgct atagatatat atcatactag tatcgtaata aaagtactct aaaagaagaa
      541 ttttcaaata cgcaaaatta agtacgaaag agttgaaaca aatttggaga aaatgagcat
      601 agaatgtctg atgagttaaa tagacctaaa aatta
//
LOCUS       DACEXQ010000418          630 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.438, whole genome shotgun sequence.
ACCESSION   DACEXQ010000418 DACEXQ010000000
VERSION     DACEXQ010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.438"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..>630
                     /locus_tag="IXH04_002921"
     CDS             <1..>630
                     /locus_tag="IXH04_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAQ1853432.1"
                     /translation="SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHY
                     PKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIR
                     AYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDS
                     IEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETN"
BASE COUNT          267 a           54 c          107 g          202 t
ORIGIN      
        1 tcattaaaaa aacattatac agagccttgg gaagatgaag tttttagagt aattattgaa
       61 tataacaatg ttcctattgg atatggacaa atatataaaa tgtatgatga gttatatact
      121 gattatcatt atccaaaaac tgatgagata gtctatggta tggatcaatt tataggagag
      181 ccaaattatt ggagtaaagg aattggtaca agatatatta aattgatttt tgaatttttg
      241 aaaaaagaaa gaaatgctaa tgcagttatt ttagaccctc ataaaaataa tccaagagca
      301 ataagggcat accaaaaatc tggttttaga attattgaag atttgccaga acatgaatta
      361 cacgagggca aaaaagaaga ttgttattta atggaatata gatatgatga taatgccaca
      421 aatgttaagg caatgaaata tttaattgag cattactttg ataatttcaa agtagatagt
      481 attgaaataa tcggtagtgg ttatgatagt gtggcatatt tagttaataa tgaatacatt
      541 tttaaaacaa aatttagtac taataagaaa aaaggttatg caaaagaaaa agcaatatat
      601 aattttttaa atacaaattt agaaactaat
//
LOCUS       DACEXQ010000419          608 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.439, whole genome shotgun sequence.
ACCESSION   DACEXQ010000419 DACEXQ010000000
VERSION     DACEXQ010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.439"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..484
                     /locus_tag="IXH04_002922"
     CDS             <1..484
                     /locus_tag="IXH04_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ1853433.1"
                     /translation="EMQTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQ
                     DGRIKPGIYSYGPDSVGEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADE
                     VAALELETKVMQDEFGKFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ
                     "
BASE COUNT          232 a           90 c          126 g          160 t
ORIGIN      
        1 agaaatgcaa acggtggtta aaaaatgtgg agcgacagaa gtaaaatcaa aatctaaacc
       61 aatcaatatg acggtaacaa ttactgcaca tgtaccgatg gaagtttatc gacgtttcaa
      121 tgggttgaaa caagatggac gtattaaacc aggcatttac tcttacggtc ctgattccgt
      181 aggcgaagat ttctcacttg ctgcagagat cgtggatgac ttcgaagaaa atagaaagtt
      241 agttggtatg ttagcatgca cttcgaatac aggattaaca ttctctattg aaaatggtgc
      301 ggatgaagta gctgcgttag aactagaaac aaaagttatg caagatgaat ttggtaaatt
      361 ttatcatgaa gcaattgttg cagaacttga agaagactta acagatcaat ggatgacgaa
      421 tctatctgct gatgtgatta aaaaaacttc agttgtgaca actacggcca ctcaatcaca
      481 gtaaaaaaaa cggaggtagc gaaatgaacg aagattactc aaaaattgaa ctaaacgatg
      541 gaacaatttt gaatttagaa cctaaactga atatcaagaa attattgatg atcaatagag
      601 attttaac
//
LOCUS       DACEXQ010000420          586 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.440, whole genome shotgun sequence.
ACCESSION   DACEXQ010000420 DACEXQ010000000
VERSION     DACEXQ010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.440"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..>586
                     /locus_tag="IXH04_002923"
     CDS             <1..>586
                     /locus_tag="IXH04_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ1853434.1"
                     /translation="RELHDRYAAIYMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVL
                     SYAIAPTESITIWEEILLDLQERGLKNVLLFITDGLKGMVGAISRFYPKARFQHCCVH
                     VSRNISHKVRVDDRKEVCDDFKMVYQASSKEVALEARGTFAEKWKTSYPKVVESILSN
                     DHLLTFYDFPLAIRKSIYSTNLIESFNKQIKKYS"
BASE COUNT          166 a          116 c          137 g          167 t
ORIGIN      
        1 ggcgggagct tcatgaccgt tatgctgcta tttatatgga cgcaacgtat attccgttaa
       61 agcggaaaac cgtcgccaag gaagctattc atatcgcagt tggcattcgc ccggacggat
      121 caaaggaagt attgagctat gcgattgcac cgactgaatc catcacgatt tgggaggaaa
      181 ttttattgga ccttcaagag cgcggtttga aaaatgtcct cctgttcatc acggatggct
      241 taaaggggat ggtaggagcg attagtcggt tctatcccaa agctcgtttt caacattgtt
      301 gtgtacacgt ttcccgtaat atcagtcata aagtgcgtgt cgatgatcgt aaggaagtct
      361 gtgatgattt taaaatggtg tatcaagcct catctaaaga ggtggcgttg gaagcacgtg
      421 gtacttttgc ggaaaaatgg aaaaccagct atccaaaagt ggttgaatcg attctttcga
      481 acgatcactt gctcactttc tatgatttcc ccttggccat acgcaagagt atttattcta
      541 cgaacttgat tgaatccttt aataaacaaa tcaagaaata cagcca
//
LOCUS       DACEXQ010000421          586 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.441, whole genome shotgun sequence.
ACCESSION   DACEXQ010000421 DACEXQ010000000
VERSION     DACEXQ010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.441"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            25..411
                     /locus_tag="IXH04_002924"
     CDS             25..411
                     /locus_tag="IXH04_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ1853435.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT          220 a           93 c          118 g          155 t
ORIGIN      
        1 taaaaataaa aaaggagatc tttcatgaaa aaaattttag ggttgatcgt tatcatattg
       61 attgctttta taggatggaa aggttgggac tactatcagt ctacttatgt aggtaaagat
      121 tattatgcgg tagtcaaagc tccaatgcct gcagaaactg atatcaaagc agataatggg
      181 gaagtaattg gaagaggttt taaatacaac gtagatgctt atgctgaaaa cggtgaaaaa
      241 agacagttag atttcgatgt cattacttct ggtgactctg ccaatggttc tgcttacccc
      301 gaaggaacga tcttgcagtt gaaagcaagc gaaaagcgga tcatagagaa aaaagtaatt
      361 accagcgata aggtaccctc atctgtaaaa gaaaaattag gcctatctta agctaagaat
      421 atatcgcaaa aaagcactcg aagtgagtgc ttttttgctt actgattcta gaaaacaaaa
      481 aaacagaatt aaccttcgaa gtcagcctca ctgttcttaa agtcctaaaa caaactaaag
      541 agcaaggcaa tcaatcgatt tctatacaaa caagggactg tgacaa
//
LOCUS       DACEXQ010000422          585 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.442, whole genome shotgun sequence.
ACCESSION   DACEXQ010000422 DACEXQ010000000
VERSION     DACEXQ010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.442"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(313..>585)
                     /locus_tag="IXH04_002925"
     CDS             complement(313..>585)
                     /locus_tag="IXH04_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1853436.1"
                     /translation="ANPDVLILDEATSSVDTRTEILIQKAMNRLLENRTSFVVAHRLS
                     TIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQFSEEEAS"
BASE COUNT          169 a          112 c          124 g          180 t
ORIGIN      
        1 caagaggttg tgagcctgcc gtttaactcc gagtcacaag gagcaatttc aaaaaacagc
       61 ttctcaggtt tgttgactat tgtgacttgt gaccgaggag ttggcaggag aacaccgttt
      121 aaggaagagg ttgtaaaatc agcggtttga cctgagtatt agagatacaa aacggaaaaa
      181 tagcttctca tattttgccg ttttttgagc taataccgaa ggacagctga ttgaacaccg
      241 tttattcgga aaaagggact gtgacacgac ttttgtcaca gcccctaaaa aaaataataa
      301 atttgtacta ttctatgaag cttcttcttc agaaaactga ctgttataaa gatctgcata
      361 gaatccgtct ttttccatca atgtatcgtg gtttcctgtt tctacgatcg aaccatgatt
      421 catcacaata atgttgtctg catcgcgtat tgtagacaag cggtgagcaa caacgaaact
      481 tgtccggttt tccaataggc ggttcatcgc tttttgaatc agtatttctg ttcgtgtatc
      541 tacactagat gttgcttcat caaggatcag gacatctgga tttgc
//
LOCUS       DACEXQ010000423          583 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.443, whole genome shotgun sequence.
ACCESSION   DACEXQ010000423 DACEXQ010000000
VERSION     DACEXQ010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.443"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(308..571)
                     /locus_tag="IXH04_002926"
     CDS             complement(308..571)
                     /locus_tag="IXH04_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circular bacteriocin, circularin A/uberolysin
                     family"
                     /protein_id="HAQ1853437.1"
                     /translation="MKKNLLLVLPILGFAGFFVGVPMLSANIGISSYAAKKVIDIINT
                     GSTVATIISIVAAVVGGGLITAGIVATAKSLIKKYGAKYAAAW"
BASE COUNT          224 a          100 c           78 g          181 t
ORIGIN      
        1 tttgtatgga ttttttcatt tacacaaaat attttatact ctcctgaatt ttttcatgtt
       61 gaaaacatca ctaatcaaag gcaaattata atttagaaat agaatgaaaa cactaaatga
      121 aaatagaaca ttagaaaaaa tagaatcaaa aaaacccaag actagtgccg aaataaggga
      181 aacaatcgaa gctacaaaaa ataatgattt cgttgaacag gaccaattta tttgtttccg
      241 atatttctca aaaagaactt tctttgggaa agttaaaggc gactgtaaag tcgcctttag
      301 tcctttttta ccaagctgct gcatatttag ctccatattt tttaattaat gatttggctg
      361 tagcaacaat accagctgta attaatccgc ctccaacaac tgcagcaaca atagaaatga
      421 ttgtagcaac cgtgctacca gtattaatta tgtcaataac ttttttagct gcataagaag
      481 aaattccaat gttagcactt agcattggta caccaacaaa gaatcccgca aaacctaaaa
      541 ttggtaaaac taataataaa ttctttttca taataattcc tct
//
LOCUS       DACEXQ010000424          563 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.445, whole genome shotgun sequence.
ACCESSION   DACEXQ010000424 DACEXQ010000000
VERSION     DACEXQ010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.445"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..129
                     /locus_tag="IXH04_002927"
     CDS             <1..129
                     /locus_tag="IXH04_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ1853438.1"
                     /translation="SGITMEKTKKGLLKQWDRKSHELFISILKELEEHENWYAEVE"
     gene            130..>563
                     /locus_tag="IXH04_002928"
     CDS             130..>563
                     /locus_tag="IXH04_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853439.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYP"
BASE COUNT          233 a           80 c          119 g          131 t
ORIGIN      
        1 tcaggtatta cgatggagaa gacaaagaaa ggtctcttaa aacaatggga tcgcaaaagc
       61 cacgaattat ttatcagtat tttgaaagaa ttggaagaac acgagaattg gtatgcggag
      121 gtggagtaat tggctgacat caaaggactt gatatttttg ggaacaacaa aggactcgcc
      181 aaaataaaaa aagaagcaga agcaaagaaa aagcagcaag caattacacc atctgaaaat
      241 aagcaattag aagtgcacca agttcaagaa ataaaaactt cctccaatgt tgaaagacag
      301 agtagaggaa gaacaaaaaa agttggcgca ccaatcagaa agtttgataa ggtttattcc
      361 accaagcatc ctttaaaact gtcgactttg ttaaatgcga cttccagagt tttagtagaa
      421 aagtatgaga ctaatatgac aagagatgaa ttgcttagaa aagctttaga tgagtatatt
      481 aaacagaatc ttacgcaaga agataagcaa gatttgttta atgacgtggt gaaagaacta
      541 gacttgttca gagaaaaata tcc
//
LOCUS       DACEXQ010000425          557 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.446, whole genome shotgun sequence.
ACCESSION   DACEXQ010000425 DACEXQ010000000
VERSION     DACEXQ010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.446"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..527
                     /locus_tag="IXH04_002929"
     CDS             <1..527
                     /locus_tag="IXH04_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HAQ1853440.1"
                     /translation="EYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDAD
                     GLTLDIWLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGT
                     EHRTIKYLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGF
                     SVCTEIKVLLGIPA"
BASE COUNT          202 a           98 c          107 g          150 t
ORIGIN      
        1 aagaatatgg caaactactc tatcaaattt ggaaaaagaa aaataaaaaa tccttttatt
       61 catggaaaat ggatgaaacg tacatcaaaa ttaaaggaaa atggcattat ttgtatcgag
      121 ccatcgatgc agatggttta accttggata tttggttacg taaaaaacgg gacacacaag
      181 cagcctatgc ttttcttaag cggttagtga agcagtttga tgaaccgaag gttgtagtca
      241 cagataaagc cccctctatt acaagtgcct ttaagaaact aaaagaatac ggcttttatc
      301 aagggacaga acatcgtacc attaaatacc tgaataattt gattgaacaa gaccatcgtc
      361 cagtaaagag acgcaataaa ttctatcgaa gtttacgcac tgcctctacc acgattaaag
      421 gcatggaagc cattcgagga ttatataaga aaacccgaaa agaaggcact ctcttcgggt
      481 tttcggtctg tactgaaatc aaggtattat tgggaatccc agcttaaatc atagataccg
      541 taagggattt tattctt
//
LOCUS       DACEXQ010000426          537 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.448, whole genome shotgun sequence.
ACCESSION   DACEXQ010000426 DACEXQ010000000
VERSION     DACEXQ010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.448"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            79..>537
                     /locus_tag="IXH04_002930"
     CDS             79..>537
                     /locus_tag="IXH04_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1853441.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYR"
BASE COUNT          202 a           83 c          106 g          146 t
ORIGIN      
        1 aaaataggca tggctattta caacaatagt gctatagtag aaaacaaatg aataaggtta
       61 cttaaagagg tgaatacgat gaaactgatc aaactatggg aagcagattt aaagaaagct
      121 tatcagctac aaactagctt tggaccaaat gagagtggat ttatcaacaa tgcttatggc
      181 tatacatttg ctgaattttt agattatgta aaaaaatgcg aaaaaacgtc tttaggaatc
      241 gatcttcctg agagttacgt tcccgcaacc gtctttattt tagaaagtga tgaacatgag
      301 tatgtaggaa tttttaattt gcgccataaa ctgaacgaaa cattaagaaa cggcgctggc
      361 catattggtt acggcatctc ccctaattat cgtaaaaaag gatatgccac acaaggatta
      421 gcattagtac tacaagaggc aaaaaaaatc ggcattgatg aggcactttt gtctgtaaat
      481 aaaaaaaatc ttggcagtct taaagcacaa caaaaaaatg gggctgcgat ttatcgt
//
LOCUS       DACEXQ010000427          536 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.449, whole genome shotgun sequence.
ACCESSION   DACEXQ010000427 DACEXQ010000000
VERSION     DACEXQ010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.449"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..>536
                     /locus_tag="IXH04_002931"
     CDS             <1..>536
                     /locus_tag="IXH04_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853442.1"
                     /translation="NDAIDQLKNGGEISDLINDIVDHQTDMITGQNGGYVLLDPKEAP
                     SRILIMDTPDKNTARNVLQINNAGIGFSKTGINGTYETAWTLDGGFNASFITAGEIVG
                     ITIRGTTLISDGADYRTSIANGKMTWYSKKVNKDIMELEARDYVSADAGIVSYTMKTG
                     GGFMIRNPQGNLVFSTWD"
BASE COUNT          178 a           70 c          121 g          167 t
ORIGIN      
        1 ttaatgatgc cattgatcaa ttgaaaaatg gtggcgaaat cagtgattta atcaatgata
       61 ttgtagatca tcaaactgat atgattactg gtcaaaatgg tggttatgtt ttattagatc
      121 ctaaagaagc gcctagtcgt attttgatta tggacacacc agataagaat accgcaagga
      181 atgttttaca aatcaacaat gctggtattg gtttttctaa aactggcatt aatggaacat
      241 atgaaacggc atggacgtta gatggcggat tcaatgcctc gtttattacg gctggtgaaa
      301 tagtaggaat tactattaga ggtactacat taattagtga tggtgctgat tatagaacaa
      361 gtattgctaa tggcaaaatg acttggtact caaaaaaagt taacaaagat attatggagc
      421 tagaagcacg tgattatgta agtgctgatg ccggtattgt atcatacacc atgaaaactg
      481 gtggtggttt catgattaga aatccacagg gtaacttggt ttttagtacg tgggat
//
LOCUS       DACEXQ010000428          527 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.450, whole genome shotgun sequence.
ACCESSION   DACEXQ010000428 DACEXQ010000000
VERSION     DACEXQ010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.450"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            258..527
                     /locus_tag="IXH04_002932"
                     /pseudo
     CDS             258..527
                     /locus_tag="IXH04_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350974.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
BASE COUNT          181 a           94 c           76 g          176 t
ORIGIN      
        1 ttttacactc tctattattc ttttcataaa tattgtaaat agaagtatta aaaatatata
       61 caaaaagtat tatttcgaga aagataaata ttttcaaaaa ccttttcata atcctcaccc
      121 tttcttacac tgataaatca tgcgtctaat attcaaatta tactcgatat gctttagcta
      181 atcagccaac tctgtttgtc tactatcatt atatcaatag acaatatctg ctcccgtctt
      241 tatgatcgtg ccaaagcaat caaaagaaaa ttgcgaatca cttagatcga aagtttactc
      301 aaacaaaagc attgtcagcg gttgtcacgg atttaactta tgttcgggtc gggacaaatt
      361 gggcttatat ttgtttgatg attgatttat tcaatcgtga aattattggc cattcttgcg
      421 gaaataaaaa agatgcgcag cttgtaaaaa gggccattca aagcattcct tactcacttc
      481 aagaaatcga actcttccat acggatcgtg gaaaagaatt tgataac
//
LOCUS       DACEXQ010000429          525 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.451, whole genome shotgun sequence.
ACCESSION   DACEXQ010000429 DACEXQ010000000
VERSION     DACEXQ010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.451"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            239..>525
                     /locus_tag="IXH04_002933"
     CDS             239..>525
                     /locus_tag="IXH04_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853443.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVL"
BASE COUNT          151 a           81 c           75 g          218 t
ORIGIN      
        1 tacgaccgga cggttatgag ccgtctgctc taaccaactg agctaaaggt acatcataaa
       61 taaaatttat ttttgttact tttcaactat agcacgaatg acaaactctc acaagtttca
      121 aaatataagt aaaataatat tgttattctt atttctttta cttgtgtcat aactattttg
      181 ttactttaat ttttatttgc ttattttaga aaaaatgtta ttcttttatt tattctcaat
      241 gttaggagtg attattttgc catttattgc gattttattc gatttagttg cggcagcagc
      301 ttattttctc caattaaatt atcaaacctc aggaattctg tttattggaa tgatttttca
      361 aggtgtgatt acccttttgc ttcttgtcat gatgctcagc tataaaggaa aaagatatgc
      421 ccgtattcaa acaaaagtat ttgttaaata tgtttccatt cgctatggga tcatcattct
      481 ttccttttta gtgaatgcga tcgtcctctt tttatatgta ttgaa
//
LOCUS       DACEXQ010000430          523 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.452, whole genome shotgun sequence.
ACCESSION   DACEXQ010000430 DACEXQ010000000
VERSION     DACEXQ010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.452"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(241..313)
                     /locus_tag="IXH04_002934"
     tRNA            complement(241..313)
                     /locus_tag="IXH04_002934"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(278..280),aa:Thr,seq:ggt)
BASE COUNT          198 a           82 c           76 g          167 t
ORIGIN      
        1 ttgaacaatg aaagactttt gcaagaataa ctattgggta gataagagat tacatctttc
       61 catgattgtt cgtcttggcc tagaccatgc aaaaaaacaa ttttcatatt gctctccttt
      121 ttatagttat catatagact ttctttgtac tattaaaata taaacattca tttcttacgt
      181 cataaaataa aaaaacagcg cagacacatt gttaaaccaa catgttgcac tgtttaagga
      241 agccacttgc cgggcttgaa ccggcgacct cttccttacc atggaagcgc tctaccagct
      301 gagctaaagc ggcattatca ttaatacaaa taataggata acgaatatta atgtatttgt
      361 caacagaatt ttttcgaaaa aatgtgtttc tctaatagga aaacagattt tagaaaaaat
      421 aaaaataaca aaaaatgcga acggtttctt tgataatata ataaaaaaat tcaaaaaaag
      481 tattgcgtta tttagaagaa attgatatta tattcatgta ctt
//
LOCUS       DACEXQ010000431          517 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.453, whole genome shotgun sequence.
ACCESSION   DACEXQ010000431 DACEXQ010000000
VERSION     DACEXQ010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.453"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            60..>517
                     /locus_tag="IXH04_002935"
     CDS             60..>517
                     /locus_tag="IXH04_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HAQ1853444.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLG"
BASE COUNT          233 a           58 c           85 g          141 t
ORIGIN      
        1 ctttcctcat atatgagctt ctacaaaaaa aatcatatca cagaaatgag gaagaaaaaa
       61 tggaaaaaga gattttaata aaatctccag gaaaaacctt gattaaagta actgatgatt
      121 ctatttcaat tattagaaaa ggatttataa atttagttaa tcaaggtatt aaaggagaaa
      181 aaactatccc ctttaaaaat atatcagccg tacaattaaa aaaaccaggt atgagtaatg
      241 gttatataca atttaccttg ctaggcggaa acgaaagtcg cggaggtata ttagcggcca
      301 cgaaagatga aaacaccgtt atgtttacaa aaaaatattg gaatgaaatg gaaaatttaa
      361 aaaaatatat agagaagcaa caatctatat tagataataa ttccaaagaa acacaaattt
      421 caagtgcaga tgaaattaaa aagtataaag agttgttaga tgaagggatt attaatcaag
      481 aagaattcga ttttaagaaa aaagaattgc ttggttt
//
LOCUS       DACEXQ010000432          514 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.454, whole genome shotgun sequence.
ACCESSION   DACEXQ010000432 DACEXQ010000000
VERSION     DACEXQ010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.454"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
BASE COUNT          176 a           67 c          105 g          166 t
ORIGIN      
        1 ggtatgtggt taaaaaagaa aaatagctag gagatttttg agagatgact ggtaaattta
       61 aaaatgatag aaatggagag tatgtatgag taataaacaa tttttatggt cttcttttgc
      121 atttattgtt gttgatattt ttttccgata ttttaataac gatatgatat ttaactggca
      181 aagaattata atcgattact gtattttttg tataatatat tttttagtga aaacatttct
      241 aaaatacgca aaaaaataaa attgaagatg ttattaaatc gcgattattt acggagattt
      301 atataaataa aaaccccctg acgaagtcga agggggtttt tattttggtt tgaggtttgc
      361 atacctaccg aaaaggtagg gcagcaaatc gtcaaacagg tatcagctaa ttgcaccata
      421 agggtgcgct gatactcggc tcaaaagagc cgaaagagcc gtctgcaaga cccctcggcg
      481 agcccacact ttgcttgcaa agtatagtgg gtta
//
LOCUS       DACEXQ010000433          514 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.455, whole genome shotgun sequence.
ACCESSION   DACEXQ010000433 DACEXQ010000000
VERSION     DACEXQ010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.455"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..287
                     /locus_tag="IXH04_002936"
     CDS             <1..287
                     /locus_tag="IXH04_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ1853445.1"
                     /translation="HELYNDISISKDPKYSDILEVLQKVYLKLEKQKYELDPSPLINR
                     LVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQF"
     gene            391..>514
                     /locus_tag="IXH04_002937"
     CDS             391..>514
                     /locus_tag="IXH04_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HAQ1853446.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVF"
BASE COUNT          215 a           47 c           85 g          167 t
ORIGIN      
        1 ttcatgaatt atataatgat atatctataa gtaaagatcc taaatattct gatattcttg
       61 aggttttaca aaaggtatat ttaaaattag aaaaacaaaa atatgaatta gatcccagtc
      121 ctttaataaa tagattggtg aattatctat attttactgc ttatactaat aaaataagat
      181 tcactgaata tcaagaggaa ttaataagaa atttgagtga aattggaaga actgctggaa
      241 taaatggttt atatcgagca gattatggag ataaatctca attttaatga tacttataat
      301 attaaatatg tctcaaaatt ttttgattag aaaaataggt atattgaatc agtgacataa
      361 aagtatttat agtttgtagg gggaatcgta atggaagaaa aaaatagact aaatgctaaa
      421 caatgttcag atcaagagct taaaaaaatt aaaggaggtg ctggaacaaa acctcaagga
      481 aaaccggcat caaatttagt ggaatgtgtt tttt
//
LOCUS       DACEXQ010000434          512 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.456, whole genome shotgun sequence.
ACCESSION   DACEXQ010000434 DACEXQ010000000
VERSION     DACEXQ010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.456"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(17..>512)
                     /locus_tag="IXH04_002938"
     CDS             complement(17..>512)
                     /locus_tag="IXH04_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAQ1853447.1"
                     /translation="TDTVGFIQDLPTQLIDAFHSTLEESQNMDLLLHVVDASSPDRIL
                     QEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLISTQTKEGKMALIEA
                     IKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEYEVKGFAKTNSKWL
                     RKNQ"
BASE COUNT          131 a          104 c           95 g          182 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg tt
//
LOCUS       DACEXQ010000435          507 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.457, whole genome shotgun sequence.
ACCESSION   DACEXQ010000435 DACEXQ010000000
VERSION     DACEXQ010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.457"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..>507)
                     /locus_tag="IXH04_002939"
     CDS             complement(<1..>507)
                     /locus_tag="IXH04_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAQ1853448.1"
                     /translation="LLLSLFPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGP
                     AIKDLLTQSSGGLLSVSALAALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIV
                     IVLFMIAISGVTIVLGLGQLILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLI
                     YLIVPNAQ"
BASE COUNT          189 a           96 c           95 g          127 t
ORIGIN      
        1 actgagcatt agggacaatt aaataaatca agaacatgat gacaaataaa gctaccaatg
       61 tgattggcca cttcaaagtt tggaactgat cgataaagtt cacgggtatt tgtacgattg
      121 gctgaatagc atccaaaatc agctgtccca acccaagaac gatagttacc ccactaatag
      181 caatcatgaa caagacaatg acaattagtg agaagaaacg gacgatcaaa aaattttttc
      241 tattttctac gccatacgct ttattcatcg caatttgcaa agcattgata ctttgacttg
      301 cagaccataa agccgctaat gcagaaacgg ataacaagcc tcctgaactt tgtgtcaaaa
      361 ggtctttgat tgcaggaccg ataaactggt aaacggtctc aggaatcact tctcgtaaat
      421 aggtcagtac agtctcttga tcgatgtgga gaaacggtaa aatattacca aaagcaataa
      481 tcaatggaaa taaagacaag agtaaat
//
LOCUS       DACEXQ010000436          502 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.458, whole genome shotgun sequence.
ACCESSION   DACEXQ010000436 DACEXQ010000000
VERSION     DACEXQ010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.458"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(207..437)
                     /locus_tag="IXH04_002940"
     CDS             complement(207..437)
                     /locus_tag="IXH04_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853449.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
BASE COUNT          172 a           94 c           55 g          181 t
ORIGIN      
        1 agctaattaa gaaacaaact ttagaaactc tagaagaact tctgaattta tatagaagtg
       61 tgtagctact tactaaatat acaaaagtta aaataaaata ctctggatac tctttatact
      121 gcattaaagt tagtaatatt aacagcaaag atatcgaaaa aacaataaca taatatgtgt
      181 caaaattttt cataataaga actcccttac tgttgcatac taacataatc caaaaactct
      241 tctacttcag ctaaagacat tccactttct ttagtccaaa aaacagtttt ttctatcgat
      301 ccttctctgt aaactgtaaa tgtcggagtc gagttaattg agtagcgact cttgaattct
      361 ttccactcat tttcatcttc tctaactttt gctacattca cgtaataaat attttctttt
      421 cccttttcta ccagcatatc gaatagcttc ctgcttttga tgatcgattt cttgtgctac
      481 atctagtaaa ttttcatcaa tt
//
LOCUS       DACEXQ010000437          493 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.460, whole genome shotgun sequence.
ACCESSION   DACEXQ010000437 DACEXQ010000000
VERSION     DACEXQ010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.460"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
BASE COUNT          150 a           76 c           74 g          193 t
ORIGIN      
        1 gaaaaagtat atagggttgt tggttggtat gtatatagaa tagaagataa atgtgaagaa
       61 attgtgcatt atttcttcac atttattagg ttatagttta agaaagttta aacattgcgc
      121 aatttaatta ttttagctgt ttcttttcat aaaaaaacat gttgtgactc tgagacaaat
      181 catagtccat agtgatgtct ctagagtaac tacgcctaat attacgaggc atcatattaa
      241 tctatcagca ctagtaccta aactagcatc cctttcgaat attttttctt atataataaa
      301 gtttgctttc actcctctaa tctcaccaaa tcgactggct tgcgttgtgt gatactagga
      361 actgattggt ttagaacgac ttttctattg taatcatatt tttcgccatc ctcgttgtct
      421 tctaatcctt caatgaatga tccctaatgt gtgtattaaa taatttaatt tttttgttcc
      481 tatttattta aca
//
LOCUS       DACEXQ010000438          489 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.461, whole genome shotgun sequence.
ACCESSION   DACEXQ010000438 DACEXQ010000000
VERSION     DACEXQ010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.461"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            127..>489
                     /locus_tag="IXH04_002941"
     CDS             127..>489
                     /locus_tag="IXH04_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853450.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNA"
BASE COUNT          178 a           60 c           83 g          168 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgct
//
LOCUS       DACEXQ010000439          479 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.462, whole genome shotgun sequence.
ACCESSION   DACEXQ010000439 DACEXQ010000000
VERSION     DACEXQ010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.462"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..405)
                     /locus_tag="IXH04_002942"
     CDS             complement(<1..405)
                     /locus_tag="IXH04_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853451.1"
                     /translation="MGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEINRKIYWVS
                     IPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELIFSLTTPGN
                     ALRAAHEINKWFPEYKNFNFLNKLDLGISSFGK"
BASE COUNT          179 a           77 c           60 g          163 t
ORIGIN      
        1 ctttccaaat gatgaaatac ctaaatctaa cttattaaga aagttgaaat ttttatattc
       61 aggaaaccat ttattaattt catgtgcagc acgtaatgca ttccctggag ttgtcaaaga
      121 gaaaattaat tctgctaagc ttattattga aaatactgat aacttataat tatatcgtcc
      181 attgacaatc aaatataaac taactatact tgttagaaca aaaaaacagg cgttgacttg
      241 ttcttgatta gttaaaaata tcaataaagg aatacttacc caatatattt ttctatttat
      301 ttcttctcct cttaagagtt gaaaaaacgg ataaaaagtt agtaaactaa atgctactgg
      361 ccactgatag ttcaaggtgg ttgctctcca accggtttcc cccattactg tcaatggtat
      421 taaacagtat atagagcaaa ccaataataa atttttaaca tttattttct caaaaacat
//
LOCUS       DACEXQ010000440          475 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.463, whole genome shotgun sequence.
ACCESSION   DACEXQ010000440 DACEXQ010000000
VERSION     DACEXQ010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.463"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..>475
                     /locus_tag="IXH04_002943"
     CDS             <1..>475
                     /locus_tag="IXH04_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ1853452.1"
                     /translation="TPISDFLKNTWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIW
                     TSISTTASNIWNSISATASSIWNSIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSI
                     SSTASNIWSSVTSSISNAINAAKSAIQSVWNSISSWISGIWNGIKNTALNLWNGIT"
BASE COUNT          155 a           76 c           97 g          147 t
ORIGIN      
        1 gacacctatt tctgatttcc ttaaaaatac ttgggatact atgacacaat ggataagcat
       61 cgcttggaat ggaattgtaa ctacattcca tactatattt gatccagtag tggcatggtg
      121 gaatggtata tggacatcta ttagtactac ggcttcaaat atttggaact caattagtgc
      181 aacagcttct agtatttgga atagtatcaa gaatacaatc actagcttgg tacaagcagc
      241 tgctacagta attcaaaata tttggtcaac tgtatctagt tggttaggtg gaatttggaa
      301 ttcaatcagc tctacagcat caaatatctg gagtagcgtg actagtagta taagcaatgc
      361 tataaacgca gctaaaagtg ccattcaaag tgtttggaat agtatatctt cgtggatcag
      421 cggaatttgg aacggtatca aaaacactgc tttgaatctt tggaatggaa ttaca
//
LOCUS       DACEXQ010000441          474 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.464, whole genome shotgun sequence.
ACCESSION   DACEXQ010000441 DACEXQ010000000
VERSION     DACEXQ010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.464"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..193
                     /locus_tag="IXH04_002944"
     CDS             <1..193
                     /locus_tag="IXH04_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ1853453.1"
                     /translation="YSTNLIEGFNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKF
                     LGRIHKGFKEIQDTLESMI"
BASE COUNT          173 a           76 c           54 g          171 t
ORIGIN      
        1 ttattcgacg aacctgattg aaggctttaa taagcagcta aaacgatata ctcgaagaaa
       61 agaacaattc cctaatgaag aatctctaga gagattcttc atttctcaat ttaatcaata
      121 taaccaaaaa tttttaggta gaattcacaa gggatttaaa gaaattcagg atacattaga
      181 gtcgatgatt taactgtaat taacagaatg gattttccat ttacatataa ttcttgacgc
      241 taccataatc taaataacca tcatattcat ctactactta tataattgag ttttattaaa
      301 ctttttaagt ttctattcta tcaaatattt aaacaatctc tgcatccgaa aagactacct
      361 cgtatagttt tactaacaat atttttatta gcgtgttcaa atcctgattt cttgtatcaa
      421 ttattttctg ttcactttca ataagaaaaa agtctttact tccatggata ataa
//
LOCUS       DACEXQ010000442          461 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.465, whole genome shotgun sequence.
ACCESSION   DACEXQ010000442 DACEXQ010000000
VERSION     DACEXQ010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.465"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..445
                     /locus_tag="IXH04_002945"
     CDS             <1..445
                     /locus_tag="IXH04_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAQ1853454.1"
                     /translation="KIVEFINLACPYCRQWFEESYELLEEAVQSGQLQRVIKLFDKEK
                     ESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQDEWKHLSLQEVADFAVDKLKLTEQKD
                     EQLSQAVINEAEQAHIRFVPTVILGKEIFDESISIEELKELIQTK"
BASE COUNT          178 a           81 c           97 g          105 t
ORIGIN      
        1 aaaaattgtc gaattcatca acttagcttg tccgtattgc cgacaatggt ttgaagaatc
       61 ttatgagcta ttagaagaag cagtccaaag cgggcaattg cagcgagtga tcaaattgtt
      121 cgataaagaa aaagaaagct tacagcgagg gaacgtgatg caccggtatc tcacaatcag
      181 cgatggccaa aaagcgatca aagagatcaa acaaatattc gatactcaag acgagtggaa
      241 acatctttct ctccaagaag tagcggattt tgcagtagat aaactaaagc tgacagaaca
      301 aaaagacgaa cagctgagcc aagctgtcat caacgaagct gagcaagcac atattcgatt
      361 tgttccaact gtcatcttgg gtaaagaaat ctttgatgaa tctatttcaa tagaggaatt
      421 aaaagaattg atccaaacaa aataaaaaaa taaggagcgt g
//
LOCUS       DACEXQ010000443          454 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.466, whole genome shotgun sequence.
ACCESSION   DACEXQ010000443 DACEXQ010000000
VERSION     DACEXQ010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.466"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(83..>454)
                     /locus_tag="IXH04_002946"
     CDS             complement(83..>454)
                     /locus_tag="IXH04_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853455.1"
                     /translation="VDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADI
                     VKEIIQEQLLNTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFP
                     YLNQFLKECYLEKLNLRMNKT"
BASE COUNT          132 a           85 c           78 g          159 t
ORIGIN      
        1 aaaacttctg ctttcggttg aagttttctt agatttaggg cagctgtgat gcctgcatgg
       61 gaagcaccaa tgatcacgat tttcatgttt tattcatcct taaattcaat ttttctaaat
      121 aacattcttt taaaaattgg ttcaaatagg ggaaatcata attatattcg ttgctagtga
      181 atacaaaaac tttagcggac ttctgtgacg ggtactcttt caattggcta gtcaaaatga
      241 tatcatactg cttttctttc tcgtattcga ctaaatcata tttgatcgta ttcagcaatt
      301 gttcctggat gatttctttc acgatatccg ccattagcct ttcataagga agatcataag
      361 caatttgcag acgtaattct aactgcttga tcagcgtccg caaaatagat gcatagcgag
      421 caaataattc aaagcgggca ttttcctcgt ctac
//
LOCUS       DACEXQ010000444          452 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.467, whole genome shotgun sequence.
ACCESSION   DACEXQ010000444 DACEXQ010000000
VERSION     DACEXQ010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.467"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            116..452
                     /locus_tag="IXH04_002947"
                     /pseudo
     CDS             116..452
                     /locus_tag="IXH04_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002384409.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
BASE COUNT          162 a           55 c           82 g          153 t
ORIGIN      
        1 gatattatga taaattaact atggatgata tttgaaaaaa acatactgta tatttgattt
       61 aatttttatt tttaagctca tatcgtaaga atgggacttg tagtctcact tctttatgga
      121 ggtgataatt atgaacgttg gttttattgg agccggcaag gtaggttgtt cattcgggaa
      181 gtatttccag gaacataaaa tacaagtaac aggattttat agtaaaagcg aagactcgtc
      241 attagcagct tcgaatttta catctagtaa acaatattta aacttaagag aattagtaga
      301 tgaaaatgat actattttca ttacgactcc tgatggtcaa attcaggagg tatggcaaga
      361 aattaaaaat tattaaatca aaaacaaatt aatctgccat tgcagtggtg caatatcttc
      421 agatatcttt tcaaatatac aggactatgg tg
//
LOCUS       DACEXQ010000445          444 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.468, whole genome shotgun sequence.
ACCESSION   DACEXQ010000445 DACEXQ010000000
VERSION     DACEXQ010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.468"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..303)
                     /locus_tag="IXH04_002948"
     CDS             complement(<1..303)
                     /locus_tag="IXH04_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853456.1"
                     /translation="MKFLNFLNYKYLNWVLYGITLFFIIYYFFTDDDIFLILAILFLV
                     SSNFSDNLIEVLTEPEIRSMTDEEQQIIKAMISENKKDVVVIKKIREYTNLGVLQAK"
BASE COUNT          165 a           70 c           47 g          162 t
ORIGIN      
        1 tttggcttgt aaaacaccta aatttgtata ctctctaatc tttttgataa ctactacgtc
       61 tttcttattt tccgaaatca tagctttaat aatctgttgt tcttcatccg tcatagaccg
      121 aatttctggt tctgtaagaa cttcaattaa gttatcacta aaattagatg aaacaagaaa
      181 aagtattgct aaaattaaaa aaatatcatc atcggtaaaa aagtaataaa taataaaaaa
      241 taatgtaata ccatataata cccagtttaa atatttatag ttcaaaaaat ttaaaaactt
      301 cactttatct ctcttttcta tttaaaatat caaacaaaca ttttttgtag aaatcctttt
      361 ttctgctctt tcaacaaatc taacttacgt tgatgaagaa cgatagtgtc atcggttctg
      421 ttgcgaagtt tgaaaatcaa acgc
//
LOCUS       DACEXQ010000446          436 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.469, whole genome shotgun sequence.
ACCESSION   DACEXQ010000446 DACEXQ010000000
VERSION     DACEXQ010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.469"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..411
                     /locus_tag="IXH04_002949"
     CDS             <1..411
                     /locus_tag="IXH04_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853457.1"
                     /translation="GLYYSPVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAP
                     QEYLSRAYNDAPKYCANIVDTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLT
                     ELKGYHVTTGEPVCQPRRPNSRIDKVRYMFNKIT"
BASE COUNT          138 a           75 c           83 g          140 t
ORIGIN      
        1 ggcttatact atagccctgt tagtacccca gcagattttc ggatgcttgc tcctattgta
       61 ttggaacaag tcttaaaaaa agctggaaca gaattgttag agccatatct tagttttaaa
      121 atttatgcgc cacaggaata tctttcacga gcatacaacg atgctcctaa atattgtgcg
      181 aacatcgtag acactcaatt gaaaaataat gaggtcattc ttagtggaga aatccctgct
      241 cggtgtattc aagaatatcg tagtgattta actttcttta caaatggacg tagtgtttgt
      301 ttaacagagt taaaagggta ccatgttact accggtgaac ctgtttgcca gccccgtcgt
      361 ccaaatagtc ggatagataa agtacgatat atgttcaata aaataactta gtgtatttta
      421 tgttgttata taaata
//
LOCUS       DACEXQ010000447          432 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.470, whole genome shotgun sequence.
ACCESSION   DACEXQ010000447 DACEXQ010000000
VERSION     DACEXQ010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.470"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..417
                     /locus_tag="IXH04_002950"
     CDS             <1..417
                     /locus_tag="IXH04_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1853458.1"
                     /translation="TSDDKPVIDPTRAGAANPTLTDGTNHAHIDQFGEIENANLHVDG
                     WHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYHVSFNMRNFPN
                     KKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPQR"
BASE COUNT          156 a           75 c           86 g          115 t
ORIGIN      
        1 acttcagatg ataaaccagt cattgatcca actagagcag gtgcagcaaa tcctacgctg
       61 acagatggaa caaatcacgc ccacattgat cagtttggag aaatcgaaaa cgcaaacttg
      121 catgtggatg gatggcacat tgctaattat aaatacgagt atattttcat tatggattac
      181 aatactggaa aagaattagc tcgagtaaga gctgatggaa tttatagacc agatgtaaat
      241 caagcttata atacttctgg aaacgttggt tatcatgtat ctttcaatat gcgtaatttt
      301 cctaataaga aagtctatgt aatgatgcgt gcaacgaatg atccagaagg gaacactaaa
      361 ggcggagcac aagattttca tgataaacgc tggtatttaa atattccgca acgataaaaa
      421 aagcccctcg at
//
LOCUS       DACEXQ010000448          415 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.471, whole genome shotgun sequence.
ACCESSION   DACEXQ010000448 DACEXQ010000000
VERSION     DACEXQ010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.471"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..339)
                     /locus_tag="IXH04_002951"
     CDS             complement(<1..339)
                     /locus_tag="IXH04_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1853459.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DACEXQ010000449          402 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.472, whole genome shotgun sequence.
ACCESSION   DACEXQ010000449 DACEXQ010000000
VERSION     DACEXQ010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.472"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..>402
                     /locus_tag="IXH04_002952"
     CDS             <1..>402
                     /locus_tag="IXH04_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ1853460.1"
                     /translation="VKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEETVIHLFR
                     KGITMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKVRTLNDKYVAIFMDATYI
                     PLKRQTVSKEAIYIAIGIREDGTKEVLSYAIA"
BASE COUNT          130 a           90 c           84 g           98 t
ORIGIN      
        1 gtcaagaccg agtacgggga acttcatctc cagattccgc gcgaccgcaa cggcgagttc
       61 aagcaacaga ctgttcctgc ttatagacgg acgaatgata ccttagagga gaccgtcatt
      121 cacctcttcc gaaaaggtat taccatgtcg gaaatcgcag acttgattga gaaaatgtat
      181 gggcatcact acacgcccca aaccatgtcc aacatgacta aagtgctgac tgaagaagta
      241 aatgcattta aagttagaac tctaaatgat aagtatgtcg ctattttcat ggatgctact
      301 tatattcctt taaaacggca aaccgtctcc aaagaagcaa tctatattgc cattggtata
      361 cgagaagacg gcactaaaga agtactaagt tatgcgattg cc
//
LOCUS       DACEXQ010000450          390 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.473, whole genome shotgun sequence.
ACCESSION   DACEXQ010000450 DACEXQ010000000
VERSION     DACEXQ010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.473"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..>390
                     /locus_tag="IXH04_002953"
     CDS             <1..>390
                     /locus_tag="IXH04_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ1853461.1"
                     /translation="SSIISALFMSGISWTYLDILRGQKDQIEPFKDAFRGFKGLFIGG
                     VISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDIVQETGQTPKVLDTITASR
                     KLMDGYKGQLFWLDLSFIGWHILALATA"
BASE COUNT          111 a           70 c           76 g          133 t
ORIGIN      
        1 ttccagcatc attagcgctt tgttcatgag cggtatttct tggacttatc ttgatatttt
       61 acgtggacaa aaagatcaga tcgaaccatt caaagatgca tttcgtggat tcaaaggttt
      121 gtttatcggc ggagtgatca gcttagctgt gctgatctcg atctttacaa cattatgggg
      181 tttgctatta gttattcctg gaatcattaa aagctattct tattctcaaa gctattttat
      241 ttactacgat atcgtccaag aaacaggaca aacacctaaa gtactggata cgatcactgc
      301 aagtcgaaaa ttgatggatg gttataaagg acaacttttc tggttagatc tttcatttat
      361 cggctggcat atcttagcat tagcaactgc
//
LOCUS       DACEXQ010000451          384 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.474, whole genome shotgun sequence.
ACCESSION   DACEXQ010000451 DACEXQ010000000
VERSION     DACEXQ010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.474"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..363
                     /locus_tag="IXH04_002954"
     CDS             <1..363
                     /locus_tag="IXH04_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAQ1853462.1"
                     /translation="TLAAEEDFVQAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLF
                     ESGKSFWLNAPFYPTDRINGTGDSLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEI
                     NVGHKYGPINHWAAGELE"
BASE COUNT          144 a           65 c           89 g           86 t
ORIGIN      
        1 acattagcag ctgaagaaga ttttgtgcaa gcagcaaaag aactgcaaga tatgggtgca
       61 aaaaacgtta tgatcaaagg acatcatgct aaaaatgcgc agcaagaaac agtcaatgac
      121 tacgtattgt tcgaatcagg aaaatcattt tggttaaatg caccatttta tcctaccgac
      181 cggatcaacg gaactggcga ttcgctatct gcatgtattg cagctgagtt agcgaaagga
      241 aaaacagttg aacaagcgat ccgattggca aaagactatg taaaccgtgc aattcgtgaa
      301 gaaataaatg tagggcacaa atatggcccc atcaatcatt gggcagctgg tgaattagag
      361 taaaagaaag aagttgtgaa aaag
//
LOCUS       DACEXQ010000452          376 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.476, whole genome shotgun sequence.
ACCESSION   DACEXQ010000452 DACEXQ010000000
VERSION     DACEXQ010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.476"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..>376
                     /locus_tag="IXH04_002955"
     CDS             <1..>376
                     /locus_tag="IXH04_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1853463.1"
                     /translation="RKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIG
                     LVLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSF
                     KALNKAINDYAKFAYKYSLSGKT"
BASE COUNT          131 a           55 c           68 g          122 t
ORIGIN      
        1 ttcgaaagac aattgatgac aacgcggcta aaacacatat ggcaattgct cacttgattc
       61 cagatagtgc tcaagccata gtaacacctg ttttatcagt tgccttaggc tttttcatta
      121 actttaaaat tggattggtt ctaatcgcca tgttagtaat tggtgtattc ttgttaaaga
      181 aaatgacaga gaatacaaat tttattcaaa aatatcaaga atcgttagat gtgttaagct
      241 ctgaaacggt agaatatatc cgcggaattc aagttataaa gatatttggt gctaaagtaa
      301 catcatttaa agccttgaat aaagcaatta acgattatgc taagtttgca tataagtatt
      361 ctttgagtgg gaaaac
//
LOCUS       DACEXQ010000453          375 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.477, whole genome shotgun sequence.
ACCESSION   DACEXQ010000453 DACEXQ010000000
VERSION     DACEXQ010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.477"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..132)
                     /locus_tag="IXH04_002956"
     CDS             complement(<1..132)
                     /locus_tag="IXH04_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ1853464.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          132 a           69 c           76 g           98 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaat
//
LOCUS       DACEXQ010000454          368 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.479, whole genome shotgun sequence.
ACCESSION   DACEXQ010000454 DACEXQ010000000
VERSION     DACEXQ010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.479"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            82..>368
                     /locus_tag="IXH04_002957"
     CDS             82..>368
                     /locus_tag="IXH04_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAQ1853465.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCR"
BASE COUNT          130 a           59 c           76 g          103 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcg
//
LOCUS       DACEXQ010000455          367 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.480, whole genome shotgun sequence.
ACCESSION   DACEXQ010000455 DACEXQ010000000
VERSION     DACEXQ010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.480"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..>367
                     /locus_tag="IXH04_002958"
     CDS             <1..>367
                     /locus_tag="IXH04_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853466.1"
                     /translation="ATKLDDKSDADKYNPEGQKVTTELNKEPDASEAIKNKKDLPKDT
                     KYTWKEKVDVSTAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEGEKVE
                     IGGTVDLTDNVTNLPTLPEG"
BASE COUNT          155 a           52 c           79 g           81 t
ORIGIN      
        1 gcaacaaaac tggatgataa atctgacgct gacaagtata atcctgaagg tcaaaaagtg
       61 actacagaat tgaataaaga accagacgca tctgaggcaa ttaaaaataa gaaagattta
      121 ccaaaagata ctaagtatac ttggaaagaa aaagtagatg ttagcacagc tggaaataaa
      181 aaaggtactg ttgtagtgac atatccagat ggatcatctg atgaagttga agtggatgtt
      241 acagtgacag acaatcgttc tgacgccgat aaatatgaac caacagtaga aggcgaaaaa
      301 gtagaaattg gaggcacagt agatttaaca gataatgtta ctaatttgcc aacattacca
      361 gaaggaa
//
LOCUS       DACEXQ010000456          361 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.481, whole genome shotgun sequence.
ACCESSION   DACEXQ010000456 DACEXQ010000000
VERSION     DACEXQ010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.481"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
BASE COUNT          123 a           39 c           58 g          141 t
ORIGIN      
        1 taggactcaa ttgttcatag atgagcattt gtaattaagt tatattataa taaaaaaata
       61 cgaattttag tgacgccatt atattgtttt tatttttcaa ttgtaatatt tcatattact
      121 tccataataa ggagtatttt ctattggaat atcttagctc ttttaaggat ttctcctttt
      181 ttgtttctca ttttttaaga ttatttggat ttcctttaca acaaacttcc acaagtatta
      241 ttgacataga tgtaattgag ggaagggggt tccagtgcta gaataaataa tgaaaagagg
      301 taacaaatgc tagcgttaag aggtgaacaa gtttgattga aagacatgta aatttattaa
      361 a
//
LOCUS       DACEXQ010000457          349 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.482, whole genome shotgun sequence.
ACCESSION   DACEXQ010000457 DACEXQ010000000
VERSION     DACEXQ010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.482"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            141..>349
                     /locus_tag="IXH04_002959"
     CDS             141..>349
                     /locus_tag="IXH04_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853467.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCP"
BASE COUNT          111 a           58 c           72 g          108 t
ORIGIN      
        1 aaaaaagagg ttgtgacgtg gtcacgacct ctttttgacg attatgatac aaaaaacatc
       61 atttatgaac aaaatgtgaa taacgggcgt tcttgagata tgattttaat tgggaaaaac
      121 ttatcgataa ggaggtcttt atgaaaaaaa gactgttatg gtgtttgctg tttctatccc
      181 taagtttaac aagtcagcca gtttttgctg aagaaacaca gcggtctcct attacaggca
      241 cacgtctgta caaaaatgtt tatgtacaga caacagatct tgattctgcg attcctgaaa
      301 cagaagaacg gtctttgcag ccaagatttg gttacttttg gtactgtcc
//
LOCUS       DACEXQ010000458          343 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.483, whole genome shotgun sequence.
ACCESSION   DACEXQ010000458 DACEXQ010000000
VERSION     DACEXQ010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.483"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
BASE COUNT          113 a           40 c           48 g          142 t
ORIGIN      
        1 tattgtgtta ctttatgtct gtggtaatag ttatagttgc ttaccaatgt atgttcattt
       61 ttcgaaatgt tgacttcata atccttggct taatgtttga atttttttgg tgatagattt
      121 tttcagccgt ataaaataaa tcgtaattct attatagaga gttgtgatta taaaaagata
      181 gcaagaaaaa aaaagaaagt ttctatacat tttaaatatt ttattcctat ttctattgct
      241 aaaaaagtag aaattctata tttaccttta actctcgctt ctcttgcaaa tgtagttagc
      301 attttaatcg ataataaact caggtagtta tattaccttg aaa
//
LOCUS       DACEXQ010000459          338 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.484, whole genome shotgun sequence.
ACCESSION   DACEXQ010000459 DACEXQ010000000
VERSION     DACEXQ010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.484"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            1..>338
                     /locus_tag="IXH04_002960"
     CDS             1..>338
                     /locus_tag="IXH04_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853468.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLN"
BASE COUNT          127 a           45 c           66 g          100 t
ORIGIN      
        1 atgatcgata atttaataac tatttacgat aaaaatgacg cgaataattt agctgaacat
       61 ttatatgata cgcaaggttt aggcgctttg tcagactggt taacagctac tgttagcaat
      121 aaactaaacg gagccgagat atttcagggt acttattcaa taaacggaac taatgcagat
      181 ttgattgtag aaggacgtat tattcagtgt tatgtagatg aaaatcgagc aaaacagcgt
      241 ctacggattt attatgcaaa aacttctgta ataggcaata cgatagaagt aaaagctgaa
      301 cctattttca atgatataag aaaatcggtg ttgaataa
//
LOCUS       DACEXQ010000460          333 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.485, whole genome shotgun sequence.
ACCESSION   DACEXQ010000460 DACEXQ010000000
VERSION     DACEXQ010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.485"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..>333
                     /locus_tag="IXH04_002961"
     CDS             <1..>333
                     /locus_tag="IXH04_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1853469.1"
                     /translation="DVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKALF
                     EKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYLSQ
                     MGVSKEKLA"
BASE COUNT          106 a           48 c           79 g          100 t
ORIGIN      
        1 agatgttatt ggtgacggtg ggattgttta ccgtgtgggt gagcaaggat atgtttcgtg
       61 gggagctggt aacgctaatc cttatgcgcc tgtacagatt gaattacagc gcacatatga
      121 taaagcattg tttgaaaaaa actatcgagc ttatattgaa tatacaagag atagtgcaaa
      181 aaaatatgga attccattga ctcttgatca aggaacttct ttatttacaa aaggaatcat
      241 ttctcatttg tgggtgacaa attatgtttg ggggaaccac acagatccat atggttactt
      301 atcgcaaatg ggagttagca aagaaaagct tgc
//
LOCUS       DACEXQ010000461          328 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.486, whole genome shotgun sequence.
ACCESSION   DACEXQ010000461 DACEXQ010000000
VERSION     DACEXQ010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.486"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..>328)
                     /locus_tag="IXH04_002962"
     CDS             complement(<1..>328)
                     /locus_tag="IXH04_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853470.1"
                     /translation="APNFRYSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSD
                     GMLTLPGCFFGSWEDGKLARFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETP
                     EYYFADY"
BASE COUNT          104 a           74 c           49 g          101 t
ORIGIN      
        1 ataatcagcg aaataatatt ctggagtttc ataagcgttc aatagtcgtt gtccataatg
       61 ttctgttggt acagttgagt ttttagtacc tctaacagta aaatctttat atacttgtac
      121 cgttgattgt tcaaacctag caagtttccc atcttcccat gaaccaaaaa aacaacctgg
      181 taacgttagc ataccatcac tagtaaattt catagtccta ccagctacct taaattccca
      241 tgagttaccc gcactaccat taatgcttaa agaactaccg tcgccagaag ttacataact
      301 agcattgcta tacctgaaat tgggcgca
//
LOCUS       DACEXQ010000462          326 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.487, whole genome shotgun sequence.
ACCESSION   DACEXQ010000462 DACEXQ010000000
VERSION     DACEXQ010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.487"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(239..326)
                     /locus_tag="IXH04_002963"
                     /pseudo
     CDS             complement(239..326)
                     /locus_tag="IXH04_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336567.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase"
BASE COUNT          107 a           64 c           46 g          109 t
ORIGIN      
        1 tgaccaatct ttcacataaa accaggaatt gtttatcttc tacttcagtt aaccgctgtt
       61 ttaatccttc ttggccaaaa atattcatcc atcctctaaa ataggcaaaa tgagcgtgat
      121 tttgcatcac tgtcaacgca atcttttgcg aaatctcttc tggaaaaaca taaaaagaga
      181 aatattcttc tatcgattgt ttaaacgttc gattcaattg agctactacg tcgtctttgt
      241 atttttcgct tatcaaaaac tcctggacat gcgagttatg tgtatctaat gtaaagttac
      301 tgatcatgaa aagataaaaa accaac
//
LOCUS       DACEXQ010000463          322 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.488, whole genome shotgun sequence.
ACCESSION   DACEXQ010000463 DACEXQ010000000
VERSION     DACEXQ010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.488"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(44..>322)
                     /locus_tag="IXH04_002964"
     CDS             complement(44..>322)
                     /locus_tag="IXH04_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1853471.1"
                     /translation="GITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQFETLAQLRLE
                     LFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT           87 a           81 c           60 g           94 t
ORIGIN      
        1 atcctcggat ctgattttct ccggcagaag cacaatcatt caattacgct gcctctccag
       61 aggtatcaga tccgcgctca ttatcaagga ttcgcttcgc caatctctgt tgtcgaattt
      121 ggatcggtgt ttcatacgcc aacgtgccat gtaagcgtag atagttccac caatgcacat
      181 aatcaaacaa ttctagacgt agctgtgcca gtgtctcaaa ttggtattga tgcacgaatt
      241 ctactttgac agatttatag gttgattcca caacggcatt atcatacgga caacccttcc
      301 tactcaatga acgagtaatt cc
//
LOCUS       DACEXQ010000464          305 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.489, whole genome shotgun sequence.
ACCESSION   DACEXQ010000464 DACEXQ010000000
VERSION     DACEXQ010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.489"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            84..272
                     /locus_tag="IXH04_002965"
     CDS             84..272
                     /locus_tag="IXH04_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1853472.1"
                     /translation="MLKKTMLVLGSISLGVLGFSPMLLSVIGKNNSKGELKLNPNYYN
                     SPNEPMFISEKQNDGQDK"
BASE COUNT          113 a           54 c           48 g           90 t
ORIGIN      
        1 caatttaaac atttgacaaa ctatagaaca gcacttaaaa tgtgtataca agtgacagcg
       61 ctttaaaaaa agaggtgcag cctatgttaa aaaaaactat gttggtacta ggttctattt
      121 ctctaggagt attaggtttt agccccatgc tattatccgt aattggaaaa aataattcca
      181 aaggggaact taaattaaat ccaaattact acaatagtcc taatgaacct atgtttattt
      241 cagaaaaaca aaacgacggt caggacaaat aatcctgccg tcgttttgct ctaacttcta
      301 ccagt
//
LOCUS       DACEXQ010000465          296 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.denovo.490, whole genome shotgun sequence.
ACCESSION   DACEXQ010000465 DACEXQ010000000
VERSION     DACEXQ010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.denovo.490"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..>296)
                     /locus_tag="IXH04_002966"
     CDS             complement(<1..>296)
                     /locus_tag="IXH04_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ1853473.1"
                     /translation="DWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSK
                     SAMYIADWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESG"
BASE COUNT           99 a           57 c           46 g           94 t
ORIGIN      
        1 tcaccgcttt ccaacatgaa aggaataaca ccagcaacat ataaatttgt atcaaattct
       61 ttcacaaaaa agtccatata ctcaagatat tgcatcgcat ttctcatcga ttttacttgg
      121 gtctgtaaga cgacgattgc ccagtccgca atgtacatcg cggatttact atatacagaa
      181 atagaaggtg ggacatctaa atagatgacg tcaaactcat caaataatgg ttctagcttt
      241 tctttcatat atttgaattg ggataatgaa tcattatatt ggtctttgat ccaatc
//
LOCUS       DACEXQ010000466         2579 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.guided.273, whole genome shotgun sequence.
ACCESSION   DACEXQ010000466 DACEXQ010000000
VERSION     DACEXQ010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.guided.273"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            3..1922
                     /gene="tet(M)"
                     /locus_tag="IXH04_002967"
     CDS             3..1922
                     /gene="tet(M)"
                     /locus_tag="IXH04_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ1853474.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
     gene            2020..2208
                     /locus_tag="IXH04_002968"
     CDS             2020..2208
                     /locus_tag="IXH04_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1853475.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(2266..>2579)
                     /locus_tag="IXH04_002969"
     CDS             complement(2266..>2579)
                     /locus_tag="IXH04_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1853476.1"
                     /translation="LAIKEARMKRGLTREQVGTMIEIDPRYLTNIENKGQHPSTQVLY
                     DLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLMEALAKGINEARNIE
                     G"
BASE COUNT          870 a          411 c          505 g          793 t
ORIGIN      
        1 acatgaaaat tattaatatt ggagttttag ctcatgttga tgcaggaaaa actaccttaa
       61 cagaaagctt attatataac agtggagcga ttacagaatt aggaagcgtg gacaaaggta
      121 caacgaggac ggataatacg cttttagaac gtcagagagg aattacaatt cagacaggaa
      181 taacctcttt tcagtgggaa aatacgaagg tgaacatcat agacacgcca ggacatatgg
      241 atttcttagc agaagtatat cgttcattat cagttttaga tggggcaatt ctactgattt
      301 ctgcaaaaga tggcgtacaa gcacaaactc gtatattatt tcatgcactt aggaaaatgg
      361 ggattcccac aatctttttt atcaataaga ttgaccaaaa tggaattgat ttatcaacgg
      421 tttatcagga tattaaagag aaactttctg ccgaaattgt aatcaaacag aaggtagaac
      481 tgtatcctaa tatgtgtgtg acgaacttta ccgaatctga acaatgggat acggtaatag
      541 agggaaacga tgacctttta gagaaatata tgtccggtaa atcattagaa gcattggaac
      601 tcgaacaaga ggaaagcata agatttcaga attgctcctt gtaccctgtt tatcatggaa
      661 gcgcaaaaag caacataggg attgagcagc ttatagaagt gataacgaat aaattttatt
      721 catcaacata cagaaagaag tctgaacttt gcggaaatgt cttcaaaatt gaatattcgg
      781 aagaaagaca acgtcttgca tatgtacgcc tttatggcgg aatcctgcat ttgcgggatt
      841 cggttagaat atcggaaaag gaaaaaataa aaattacaga aatgtatact tcaataaatg
      901 gtgaattatg taaaattgat aaggcttatt ccggggaaat tgttattttg caaaatgagt
      961 ttttgaagct aaatagtgtt cttggagata caaagctatt gccacagaga gagagaattg
     1021 aaaatccgct ccctctgctg caaacaactg ttgaaccgag caaacctcaa caaagggaaa
     1081 tgttacttga tgcactttta gaaatctccg acagtgaccc gcttctacaa tattatgtgg
     1141 attctacgac acatgaaatt atactttctt tcttagggaa agtacaaatg gaagtgatta
     1201 gtgcactgtt gcaagaaaag tatcatgtgg agatagaact aaaagagcct acagtcattt
     1261 atatggagag accgttaaaa aatgcagaat ataccattca catcgaagtg ccgccaaatc
     1321 ctttctgggc ttccattggt ttatctgtat caccgcttcc gttgggaagt ggaatgcagt
     1381 atgagagctc ggtttctctt ggatacttaa atcaatcatt tcaaaatgca gttatggaag
     1441 ggatacgcta tggttgcgaa caaggattat atggttggaa tgtgacggat tgtaaaatct
     1501 gttttaagta cggtttatac tatagccctg ttagtactcc agcagatttt cggatgctta
     1561 ctcctattgt actggagcaa gcctttagaa aagctggaac agaattgtta gagccatatc
     1621 ttagttttaa agtttatgca ccacaggaat atctttcacg ggcatataac gatgctccca
     1681 aatattgtgc aaatatcgta aatactcaac tgaaaaataa tgaggtcatt attattggag
     1741 aaattcctgc tcgatgtatt caagattatc gcaatgattt aacttttttt acaaatgggc
     1801 ttagtgtttg tttagcagag ctaaaaggat atcaggttac cactggcgaa cctgtttgcc
     1861 agacccgtcg tctaaatagt cggatagata aagtaagata tatgttcaat aaaataactt
     1921 agtgcgtttt atgttgttat ataaatatgg tttcttatta aataagatga aatattcttt
     1981 aatatagatt tgaattaaag tggaaaggag gagattgtta ttataaacta caagtggata
     2041 ttgtgtccta tttgtggaaa taaaacaaga ctacgaatac gagtggatac tatacttaaa
     2101 aatttccctt tatactgccc caaatgtaag aacgaaactt taattaatgt tcaaaaaatg
     2161 aatataataa caatcaaaga gccagacgcc aagacgcaga gccgataatt tgagaaatga
     2221 aactctcatc ttatcggctc tttttgttta tctgaatttt actgactagc cttcaatatt
     2281 tcttgcttca ttgatacctt ttgctagtgc ttccattaag gatagttctt tgtctgtaaa
     2341 gctatccatg tatttttcta cctgtaacct tcgagtgctt ttaatcaaat tatctgtagg
     2401 taagaaaaat tcatcaatag atacatggag taatgataca agatcataca gcacttgtgt
     2461 acttgggtgt tgtcctttgt tttctatatt tgttaaataa cgtgggtcaa tttcaatcat
     2521 tgttcccact tgttcacgag tgagaccccg tttcattcga gcctctttaa tcgctaaac
//
LOCUS       DACEXQ010000467         2579 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.guided.274, whole genome shotgun sequence.
ACCESSION   DACEXQ010000467 DACEXQ010000000
VERSION     DACEXQ010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.guided.274"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            3..1922
                     /gene="tet(M)"
                     /locus_tag="IXH04_002970"
     CDS             3..1922
                     /gene="tet(M)"
                     /locus_tag="IXH04_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ1853477.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
     gene            2020..2208
                     /locus_tag="IXH04_002971"
     CDS             2020..2208
                     /locus_tag="IXH04_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1853478.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(2266..>2579)
                     /locus_tag="IXH04_002972"
     CDS             complement(2266..>2579)
                     /locus_tag="IXH04_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1853479.1"
                     /translation="LAIKEARMKRGLTREQVGTMIEIDPRYLTNIENKGQHPSTQVLY
                     DLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLMEALAKGINEARNIE
                     G"
BASE COUNT          869 a          411 c          506 g          793 t
ORIGIN      
        1 acatgaaaat tattaatatt ggagttttag ctcatgttga tgcgggaaaa actaccttaa
       61 cagaaagctt attatataac agtggagcga ttacagaatt aggaagcgtg gacaaaggta
      121 caacgaggac ggataatacg cttttagaac gtcagagagg aattacaatt cagacaggaa
      181 taacctcttt tcagtgggaa aatacgaagg tgaacatcat agacacgcca ggacatatgg
      241 atttcttagc agaagtatat cgttcattat cagttttaga tggggcaatt ctactgattt
      301 ctgcaaaaga tggcgtacaa gcacaaactc gtatattatt tcatgcactt aggaaaatgg
      361 ggattcccac aatctttttt atcaataaga ttgaccaaaa tggaattgat ttatcaacgg
      421 tttatcagga tattaaagag aaactttctg ccgaaattgt aatcaaacag aaggtagaac
      481 tgtatcctaa tatgtgtgtg acgaacttta ccgaatctga acaatgggat acggtaatag
      541 agggaaacga tgacctttta gagaaatata tgtccggtaa atcattagaa gcattggaac
      601 tcgaacaaga ggaaagcata agatttcaga attgctcctt gtaccctgtt tatcatggaa
      661 gcgcaaaaag caacataggg attgagcagc ttatagaagt gataacgaat aaattttatt
      721 catcaacata cagaaagaag tctgaacttt gcggaaatgt cttcaaaatt gaatattcgg
      781 aagaaagaca acgtcttgca tatgtacgcc tttatggcgg aatcctgcat ttgcgggatt
      841 cggttagaat atcggaaaag gaaaaaataa aaattacaga aatgtatact tcaataaatg
      901 gtgaattatg taaaattgat aaggcttatt ccggggaaat tgttattttg caaaatgagt
      961 ttttgaagct aaatagtgtt cttggagata caaagctatt gccacagaga gagagaattg
     1021 aaaatccgct ccctctgctg caaacaactg ttgaaccgag caaacctcaa caaagggaaa
     1081 tgttacttga tgcactttta gaaatctccg acagtgaccc gcttctacaa tattatgtgg
     1141 attctacgac acatgaaatt atactttctt tcttagggaa agtacaaatg gaagtgatta
     1201 gtgcactgtt gcaagaaaag tatcatgtgg agatagaact aaaagagcct acagtcattt
     1261 atatggagag accgttaaaa aatgcagaat ataccattca catcgaagtg ccgccaaatc
     1321 ctttctgggc ttccattggt ttatctgtat caccgcttcc gttgggaagt ggaatgcagt
     1381 atgagagctc ggtttctctt ggatacttaa atcaatcatt tcaaaatgca gttatggaag
     1441 ggatacgcta tggttgcgaa caaggattat atggttggaa tgtgacggat tgtaaaatct
     1501 gttttaagta cggtttatac tatagccctg ttagtactcc agcagatttt cggatgctta
     1561 ctcctattgt actggagcaa gcctttagaa aagctggaac agaattgtta gagccatatc
     1621 ttagttttaa agtttatgca ccacaggaat atctttcacg ggcatataac gatgctccca
     1681 aatattgtgc aaatatcgta aatactcaac tgaaaaataa tgaggtcatt attattggag
     1741 aaattcctgc tcgatgtatt caagattatc gcaatgattt aacttttttt acaaatgggc
     1801 ttagtgtttg tttagcagag ctaaaaggat atcaggttac cactggcgaa cctgtttgcc
     1861 agacccgtcg tctaaatagt cggatagata aagtaagata tatgttcaat aaaataactt
     1921 agtgcgtttt atgttgttat ataaatatgg tttcttatta aataagatga aatattcttt
     1981 aatatagatt tgaattaaag tggaaaggag gagattgtta ttataaacta caagtggata
     2041 ttgtgtccta tttgtggaaa taaaacaaga ctacgaatac gagtggatac tatacttaaa
     2101 aatttccctt tatactgccc caaatgtaag aacgaaactt taattaatgt tcaaaaaatg
     2161 aatataataa caatcaaaga gccagacgcc aagacgcaga gccgataatt tgagaaatga
     2221 aactctcatc ttatcggctc tttttgttta tctgaatttt actgactagc cttcaatatt
     2281 tcttgcttca ttgatacctt ttgctagtgc ttccattaag gatagttctt tgtctgtaaa
     2341 gctatccatg tatttttcta cctgtaacct tcgagtgctt ttaatcaaat tatctgtagg
     2401 taagaaaaat tcatcaatag atacatggag taatgataca agatcataca gcacttgtgt
     2461 acttgggtgt tgtcctttgt tttctatatt tgttaaataa cgtgggtcaa tttcaatcat
     2521 tgttcccact tgttcacgag tgagaccccg tttcattcga gcctctttaa tcgctaaac
//
LOCUS       DACEXQ010000468         2350 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.guided.288, whole genome shotgun sequence.
ACCESSION   DACEXQ010000468 DACEXQ010000000
VERSION     DACEXQ010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.guided.288"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..88
                     /locus_tag="IXH04_002973"
     CDS             <1..88
                     /locus_tag="IXH04_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1853480.1"
                     /translation="PENMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            121..1033
                     /gene="ant(6)"
                     /locus_tag="IXH04_002974"
                     /pseudo
     CDS             121..1033
                     /gene="ant(6)"
                     /locus_tag="IXH04_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
     gene            1030..1572
                     /gene="sat4"
                     /locus_tag="IXH04_002975"
     CDS             1030..1572
                     /gene="sat4"
                     /locus_tag="IXH04_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAQ1853481.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            1665..>2350
                     /locus_tag="IXH04_002976"
                     /note="aph(3')-IIIa"
     CDS             1665..>2350
                     /locus_tag="IXH04_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAQ1853482.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSI"
BASE COUNT          801 a          368 c          562 g          619 t
ORIGIN      
        1 gccagagaac atgatggata ttccggggat ggcggatgaa atgcgacgcc caatgatgct
       61 gattgtatcg gcaaaaaaga agatgtaata atatagaaaa aataaacgag gagtatgtaa
      121 atgagatcag aaaaagaaat gatggattta gtactttctt tagcagaaca ggatgaacga
      181 acgtattcga attgtgaccc ttgaggggtc acgcgcaaat attaatatac ctaaagatga
      241 atttcaggat tatgatatta catattttgt aagtgatata gaaccgttta tatctaatga
      301 tgactggctt aatcaatttg ggaatataat aatgatgcaa aagccggagg atatggaatt
      361 attcccacct gaagaaaagg gattttccta tcttatgcta tttgatgatt acaataaaat
      421 tgatcttacc ttattgccct tggaagagtt agataattac ctaaagggcg ataaattaat
      481 aaaggttcta attgataaag attgtagaat taaaagggac atagttccga ctgatataga
      541 ttatcatgta agaaagccaa gcgcaaggga gtatgatgat tgctgcaatg aattttggaa
      601 tgtaacacct tatgttatta aaggattgtg ccgtaaggaa attttatttg ctattgatca
      661 ttttaatcag attgttcgcc atgagctgct gagaatgata tcatggaagg tcggcatcga
      721 aacaggcttt aaattaagtg taggcaagaa ctataagttt attgaaaggt atatatccga
      781 ggatttgtgg gagaaacttt tgtccaccta ccggatggat tcctatgaaa acatatggga
      841 agcattattt ctatgccatc aattgttcag ggcggtatcc ggtgaggtgg cggaaaggct
      901 tcattatgcc tatccggagt atgataggaa tataacaaaa tataccaggg acatgtataa
      961 aaaatacact ggtaaaaccg gctgcctgga tagcacatat gccgctgata tagaagagag
     1021 gcgggaacag tgattacaga aatgaaagca gggcacctga aagatatcga taaacccagc
     1081 gaaccatttg aggtgatagg taagattata ccgaggtatg aaaacgagaa ttggaccttt
     1141 acagaattac tctatgaagc gccatattta aaaagctacc aagacgaaga ggatgaagag
     1201 gatgaggagg cagattgcct tgaatatatt gacaatactg ataagataat atatctttac
     1261 taccaagacg ataaatgcgt cggaaaagtt aaactgcgaa aaaattggaa ccggtacgct
     1321 tatatagaag atatcgccgt atgtaaggat ttcagggggc aaggcatagg cagcgcgctt
     1381 atcaatatat ctatagaatg ggcaaagcat aaaaacttgc atggactaat gcttgaaacc
     1441 caggacaata accttatagc ttgtaaattc tatcataatt gtggtttcaa aatcggctcc
     1501 gtcgatacta tgttatacgc caactttgaa aacaactttg aaaaagctgt tttctggtat
     1561 ttaaggtttt agaatgcaag gaacagtgaa ttggagttcg tcttgttata attagcttct
     1621 tggggtatct ttaaatactg tagaaaagag gaaggaaata ataaatggct aaaatgagaa
     1681 tatcaccgga attgaaaaaa ctgatcgaaa aataccgctg cgtaaaagat acggaaggaa
     1741 tgtctcctgc taaggtatat aagctggtgg gagaaaatga aaacctatat ttaaaaatga
     1801 cggacagccg gtataaaggg accacctatg atgtggaacg ggaaaaggac atgatgctat
     1861 ggctggaagg aaagctgcct gttccaaagg tcctgcactt tgaacggcat gatggctgga
     1921 gcaatctgct catgagtgag gccgatggcg tcctttgctc ggaagagtat gaagatgaac
     1981 aaagccctga aaagattatc gagctgtatg cggagtgcat caggctcttt cactccatcg
     2041 acatatcgga ttgtccctat acgaatagct tagacagccg cttagccgaa ttggattact
     2101 tactgaataa cgatctggcc gatgtggatt gcgaaaactg ggaagaagac actccattta
     2161 aagatccgcg cgagctgtat gattttttaa agacggaaaa gcccgaagag gaacttgtct
     2221 tttcccacgg cgacctggga gacagcaaca tctttgtgaa agatggcaaa gtaagtggct
     2281 ttattgatct tgggagaagc ggcagggcgg acaagtggta tgacattgcc ttctgcgtcc
     2341 ggtcgatcag
//
LOCUS       DACEXQ010000469         1973 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.guided.312, whole genome shotgun sequence.
ACCESSION   DACEXQ010000469 DACEXQ010000000
VERSION     DACEXQ010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.guided.312"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            3..1922
                     /gene="tet(M)"
                     /locus_tag="IXH04_002977"
     CDS             3..1922
                     /gene="tet(M)"
                     /locus_tag="IXH04_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ1853483.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
BASE COUNT          676 a          311 c          408 g          578 t
ORIGIN      
        1 acatgaaaat tattaatatt ggagttttag ctcatgttga tgcaggaaaa actaccttaa
       61 cagaaagctt attatataac agtggagcga ttacagaatt aggaagcgtg gacaaaggta
      121 caacgaggac ggataatacg cttttagaac gtcagagagg aattacaatt cagacaggaa
      181 taacctcttt tcagtgggaa aatacgaagg tgaacatcat agacacgcca ggacatatgg
      241 atttcttagc agaagtatat cgttcattat cagttttaga tggggcaatt ctactgattt
      301 ctgcaaaaga tggcgtacaa gcacaaactc gtatattatt tcatgcactt aggaaaatgg
      361 ggattcccac aatctttttt atcaataaga ttgaccaaaa tggaattgat ttatcaacgg
      421 tttatcagga tattaaagag aaactttctg ccgaaattgt aatcaaacag aaggtagaac
      481 tgtatcctaa tatgtgtgtg acgaacttta ccgaatctga acaatgggat acggtaatag
      541 agggaaacga tgacctttta gagaaatata tgtccggtaa atcattagaa gcattggaac
      601 tcgaacaaga ggaaagcata agatttcaga attgctcctt gtaccctgtt tatcatggaa
      661 gcgcaaaaag caacataggg attgagcagc ttatagaagt gataacgaat aaattttatt
      721 catcaacata cagaaagaag tctgaacttt gcggaaatgt cttcaaaatt gaatattcgg
      781 aagaaagaca acgtcttgca tatgtacgcc tttatggcgg aatcctgcat ttgcgggatt
      841 cggttagaat atcggaaaag gaaaaaataa aaattacaga aatgtatact tcaataaatg
      901 gtgaattatg taaaattgat aaggcttatt ccggggaaat tgttattttg caaaatgagt
      961 ttttgaagct aaatagtgtt cttggagata caaagctatt gccacagaga gagagaattg
     1021 aaaatccgct ccctctgctg caaacaactg ttgaaccgag caaacctcaa caaagggaaa
     1081 tgttacttga tgcactttta gaaatctccg acagtgaccc gcttctacaa tattatgtgg
     1141 attctacgac acatgaaatt atactttctt tcttagggaa agtacaaatg gaagtgatta
     1201 gtgcactgtt gcaagaaaag tatcatgtgg agatagaact aaaagagcct acagtcattt
     1261 atatggagag accgttaaaa aatgcagaat ataccattca catcgaagtg ccgccaaatc
     1321 ctttctgggc ttccattggt ttatctgtat caccgcttcc gttgggaagt ggaatgcagt
     1381 atgagagctc ggtttctctt ggatacttaa atcaatcatt tcaaaatgca gttatggaag
     1441 ggatacgcta tggttgcgaa caaggattat atggttggaa tgtgacggat tgtaaaatct
     1501 gttttaagta tggcttatac tatagccctg ttagtacccc agcagatttt cggatgcttg
     1561 ctcctattgt attggaacaa gtcttaaaaa aagctggaac agaattgtta gagccatatc
     1621 ttagttttaa aatttatgcg ccacaggaat atctttcacg agcatacaac gatgctccta
     1681 aatattgtgc gaacatcgta gacactcaat tgaaaaataa tgaggtcatt cttagtggag
     1741 aaatccctgc tcggtgtatt caagaatatc gtagtgattt aactttcttt acaaatggac
     1801 gtagtgtttg tttaacagag ttaaaagggt accatgttac taccggtgaa cctgtttgcc
     1861 agccccgtcg tccaaatagt cggatagata aagtacgata tatgttcaat aaaataactt
     1921 agtgtatttt atgttgttat ataaatatgg tttcttgtta aataagatga aat
//
LOCUS       DACEXQ010000470         1973 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.guided.313, whole genome shotgun sequence.
ACCESSION   DACEXQ010000470 DACEXQ010000000
VERSION     DACEXQ010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.guided.313"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            3..1922
                     /gene="tet(M)"
                     /locus_tag="IXH04_002978"
     CDS             3..1922
                     /gene="tet(M)"
                     /locus_tag="IXH04_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ1853484.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
BASE COUNT          675 a          311 c          409 g          578 t
ORIGIN      
        1 acatgaaaat tattaatatt ggagttttag ctcatgttga tgcgggaaaa actaccttaa
       61 cagaaagctt attatataac agtggagcga ttacagaatt aggaagcgtg gacaaaggta
      121 caacgaggac ggataatacg cttttagaac gtcagagagg aattacaatt cagacaggaa
      181 taacctcttt tcagtgggaa aatacgaagg tgaacatcat agacacgcca ggacatatgg
      241 atttcttagc agaagtatat cgttcattat cagttttaga tggggcaatt ctactgattt
      301 ctgcaaaaga tggcgtacaa gcacaaactc gtatattatt tcatgcactt aggaaaatgg
      361 ggattcccac aatctttttt atcaataaga ttgaccaaaa tggaattgat ttatcaacgg
      421 tttatcagga tattaaagag aaactttctg ccgaaattgt aatcaaacag aaggtagaac
      481 tgtatcctaa tatgtgtgtg acgaacttta ccgaatctga acaatgggat acggtaatag
      541 agggaaacga tgacctttta gagaaatata tgtccggtaa atcattagaa gcattggaac
      601 tcgaacaaga ggaaagcata agatttcaga attgctcctt gtaccctgtt tatcatggaa
      661 gcgcaaaaag caacataggg attgagcagc ttatagaagt gataacgaat aaattttatt
      721 catcaacata cagaaagaag tctgaacttt gcggaaatgt cttcaaaatt gaatattcgg
      781 aagaaagaca acgtcttgca tatgtacgcc tttatggcgg aatcctgcat ttgcgggatt
      841 cggttagaat atcggaaaag gaaaaaataa aaattacaga aatgtatact tcaataaatg
      901 gtgaattatg taaaattgat aaggcttatt ccggggaaat tgttattttg caaaatgagt
      961 ttttgaagct aaatagtgtt cttggagata caaagctatt gccacagaga gagagaattg
     1021 aaaatccgct ccctctgctg caaacaactg ttgaaccgag caaacctcaa caaagggaaa
     1081 tgttacttga tgcactttta gaaatctccg acagtgaccc gcttctacaa tattatgtgg
     1141 attctacgac acatgaaatt atactttctt tcttagggaa agtacaaatg gaagtgatta
     1201 gtgcactgtt gcaagaaaag tatcatgtgg agatagaact aaaagagcct acagtcattt
     1261 atatggagag accgttaaaa aatgcagaat ataccattca catcgaagtg ccgccaaatc
     1321 ctttctgggc ttccattggt ttatctgtat caccgcttcc gttgggaagt ggaatgcagt
     1381 atgagagctc ggtttctctt ggatacttaa atcaatcatt tcaaaatgca gttatggaag
     1441 ggatacgcta tggttgcgaa caaggattat atggttggaa tgtgacggat tgtaaaatct
     1501 gttttaagta tggcttatac tatagccctg ttagtacccc agcagatttt cggatgcttg
     1561 ctcctattgt attggaacaa gtcttaaaaa aagctggaac agaattgtta gagccatatc
     1621 ttagttttaa aatttatgcg ccacaggaat atctttcacg agcatacaac gatgctccta
     1681 aatattgtgc gaacatcgta gacactcaat tgaaaaataa tgaggtcatt cttagtggag
     1741 aaatccctgc tcggtgtatt caagaatatc gtagtgattt aactttcttt acaaatggac
     1801 gtagtgtttg tttaacagag ttaaaagggt accatgttac taccggtgaa cctgtttgcc
     1861 agccccgtcg tccaaatagt cggatagata aagtacgata tatgttcaat aaaataactt
     1921 agtgtatttt atgttgttat ataaatatgg tttcttgtta aataagatga aat
//
LOCUS       DACEXQ010000471         1950 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.guided.315, whole genome shotgun sequence.
ACCESSION   DACEXQ010000471 DACEXQ010000000
VERSION     DACEXQ010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.guided.315"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..176
                     /locus_tag="IXH04_002979"
     CDS             <1..176
                     /locus_tag="IXH04_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ1853485.1"
                     /translation="CGSRDQELEGLSDFLPIVLSIHLGPIGLEEAMEHRQTLEKTKIL
                     GQTLSRFFTILPK"
     gene            434..1936
                     /gene="lsa"
                     /locus_tag="IXH04_002980"
     CDS             434..1936
                     /gene="lsa"
                     /locus_tag="IXH04_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsa family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ1853486.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDKTFLDKVSTEIISLERI"
BASE COUNT          688 a          342 c          415 g          505 t
ORIGIN      
        1 tttgcggaag tagagaccaa gagctagaag gactttctga ttttctccca attgtcttaa
       61 gtattcattt gggtccaata ggtttagaag aagcaatgga acacaggcaa acattagaaa
      121 aaacaaaaat cttaggacag acacttagca gatttttcac catcctcccg aagtagttga
      181 caaaatcatt ttctttatgc tatcgtaatt tcaaatatag gacaaagtga gggatccgtg
      241 cattgaaaaa ttaagtcgat tgatgaaagc tgcattcaga ctgtaccttg tggagacaag
      301 gaagcagtgt gcagattttc gggattggct tggttttttc gatgtatggg tctttttgtg
      361 tgtatccaac ccttgcatcc tctttagcca gttctgaaaa taggactggt ctttttttac
      421 gaggtgatgg aaaatgtcta aaatcgaaat aaaaaatctg acattcggct acgacagcca
      481 aggcacatta ttatttgaac aagcaaatct aaattttgac acacaatgga aactaggact
      541 tatcggacga aacggtcgag gaaagacaac tttactgaat attctacaaa acaaactacc
      601 ttatcaaggg caagtaatcc atcagcaaga atttgcctat ttcccgcaac agacaaaaga
      661 taaagaacgt ttaacctatt acgtgttaaa tgatattacg gattttgaga tatgggaaat
      721 cgaaagagag ctccaattga tgcaaacaga tcctgaaatc ttatggagag aattcagcac
      781 actatcgggg ggagagaaga caaaagtcct actggcactt ttatttgtgg atgacactca
      841 tttcccgtta atcgatgaac caacgaatca tttggatatc tctggtagaa aacaagtagc
      901 ggcttatttg aaaaagaaaa aacaaggctt catcgtggtc agccatgacc ggggatttat
      961 cgatgaagta gtggaccatg ttttagcaat cgaaaaaagt caactggaac tttatcaagg
     1021 gaatttctct atctatgaag aacagaaaaa acttcgtgat gaatttgaaa tggctcaaaa
     1081 tgaaaaattg aaaaaagaag tcagtaggct aaagaaaaca gcagctgaaa aagccgaatg
     1141 gtctcgttcc cgagaaggag ataaaacaaa gaaacaagtc ggattcatcg atactgaatc
     1201 tagacgagtg aataaaggag cagtgggtgc tgatgctgca cggacgatga aacgatccaa
     1261 agcaatcgtg aatcggatgg agacccagat cagcgagaaa gaaaaactat taaaagatat
     1321 cgaatatatc gattctttga cgatgaatag ccaagcgtct caccataagc gacttttaag
     1381 cgtagaagat cttcaattag ggtatgaaaa tctgttattc gagccaattc attttacaat
     1441 cgagcctcat cagcgggtgg cgatttcagg tcctaacggt gcaggaaagt catccattat
     1501 ccattatctt ctgggggcat tcaacggcaa ggttatagga gaaaaatacc agccaaaaca
     1561 tctgagcatt agttatgcaa gccaaaatta tgaagacaat cgaggaacgt tggcggaatt
     1621 tgcagagaaa aaccaagtag actaccaagc atttttgaac aacctccgaa agcttgggat
     1681 ggaaagagat gtttttcata acaagatcga gcagatgagt atgggccaac ggaaaaaagt
     1741 ggaattggct aaatctttat cacagccagc tgaactatat acatgggatg aaccattgaa
     1801 ttatttggat gtcttcaatc aagaacaatt agaacaactg atcttgaacg tgaaacctgc
     1861 catgttacta gtggaacatg ataaaacctt cctggataaa gtatctactg agattatttc
     1921 tcttgagaga atctaaccct tgtaatctca
//
LOCUS       DACEXQ010000472         1950 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.guided.316, whole genome shotgun sequence.
ACCESSION   DACEXQ010000472 DACEXQ010000000
VERSION     DACEXQ010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.guided.316"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..176
                     /locus_tag="IXH04_002981"
     CDS             <1..176
                     /locus_tag="IXH04_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ1853487.1"
                     /translation="CGSRDQELEGLSDFLPIVLSIHLGPIGLEEAMEHRQTLEKTKIL
                     GQTLSRFFTILPK"
     gene            434..1936
                     /locus_tag="IXH04_002982"
     CDS             434..1936
                     /locus_tag="IXH04_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAQ1853488.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
BASE COUNT          687 a          343 c          415 g          505 t
ORIGIN      
        1 tttgcggaag tagagaccaa gagctagaag gactttctga ttttctccca attgtcttaa
       61 gtattcattt gggtccaata ggtttagaag aagcaatgga acacaggcaa acattagaaa
      121 aaacaaaaat cttaggacag acacttagca gatttttcac catcctcccg aagtagttga
      181 caaaatcatt ttctttatgc tatcgtaatt tcaaatatag gacaaagtga gggatccgtg
      241 cattgaaaaa ttaagtcgat tgatgaaagc tgcattcaga ctgtaccttg tggagacaag
      301 gaagcagtgt gcagattttc gggattggct tggttttttc gatgtatggg tctttttgtg
      361 tgtatccaac ccttgcatcc tctttagcca gttctgaaaa taggactggt ctttttttac
      421 gaggtgatgg aaaatgtcta aaatcgaaat aaaaaatctg acattcggct acgacagcca
      481 aggcacatta ttatttgaac aagcaaatct aaattttgac acacaatgga aactaggact
      541 tatcggacga aacggtcgag gaaagacaac tttactgaat attctacaaa acaaactacc
      601 ttatcaaggg caagtaatcc atcagcaaga atttgcctat ttcccgcaac agacaaaaga
      661 taaagaacgt ttaacctatt acgtgttaaa tgatattacg gattttgaga tatgggaaat
      721 cgaaagagag ctccaattga tgcaaacaga tcctgaaatc ttatggagag aattcagcac
      781 actatcgggg ggagagaaga caaaagtcct actggcactt ttatttgtgg atgacactca
      841 tttcccgtta atcgatgaac caacgaatca tttggatatc tctggtagaa aacaagtagc
      901 ggcttatttg aaaaagaaaa aacaaggctt catcgtggtc agccatgacc ggggatttat
      961 cgatgaagta gtggaccatg ttttagcaat cgaaaaaagt caactggaac tttatcaagg
     1021 gaatttctct atctatgaag aacagaaaaa acttcgtgat gaatttgaaa tggctcaaaa
     1081 tgaaaaattg aaaaaagaag tcagtaggct aaagaaaaca gcagctgaaa aagccgaatg
     1141 gtctcgttcc cgagaaggag ataaaacaaa gaaacaagtc ggattcatcg atactgaatc
     1201 tagacgagtg aataaaggag cagtgggtgc tgatgctgca cggacgatga aacgatccaa
     1261 agcaatcgtg aatcggatgg agacccagat cagcgagaaa gaaaaactat taaaagatat
     1321 cgaatatatc gattctttga cgatgaatag ccaagcgtct caccataagc gacttttaag
     1381 cgtagaagat cttcaattag ggtatgaaaa tctgttattc gagccaattc attttacaat
     1441 cgagcctcat cagcgggtgg cgatttcagg tcctaacggt gcaggaaagt catccattat
     1501 ccattatctt ctgggggcat tcaacggcaa ggttatagga gaaaaatacc agccaaaaca
     1561 tctgagcatt agttatgcaa gccaaaatta tgaagacaat cgaggaacgt tggcggaatt
     1621 tgcagagaaa aaccaagtag actaccaagc atttttgaac aacctccgaa agcttgggat
     1681 ggaaagagat gtttttcata acaagatcga gcagatgagt atgggccaac ggaaaaaagt
     1741 ggaattggct aaatctttat cacagccagc tgaactatat acatgggatg aaccattgaa
     1801 ttatttggat gtcttcaatc aagaacaatt agaacaactg atcttgaacg tgaaacctgc
     1861 catgttacta gtggaacatg atcaaacctt cctggataaa gtatctactg agattatttc
     1921 tcttgagaga atctaaccct tgtaatctca
//
LOCUS       DACEXQ010000473         1853 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.guided.322, whole genome shotgun sequence.
ACCESSION   DACEXQ010000473 DACEXQ010000000
VERSION     DACEXQ010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1853
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.guided.322"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..668
                     /gene="vanR"
                     /locus_tag="IXH04_002983"
     CDS             <1..668
                     /gene="vanR"
                     /locus_tag="IXH04_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAQ1853489.1"
                     /translation="EHEIADLVELYLKNENYTVFKYYTAKEALECIDKSEIDLAILDI
                     MLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITKPFRPLELIAR
                     VKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPTEFSILRILCE
                     NKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKYIKTVWGVGYK
                     IEK"
     gene            646..1800
                     /locus_tag="IXH04_002984"
     CDS             646..1800
                     /locus_tag="IXH04_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAQ1853490.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          604 a          359 c          409 g          481 t
ORIGIN      
        1 atgaacatga aattgccgat ttggttgaat tatacttaaa aaacgagaat tatacggttt
       61 tcaaatacta taccgccaaa gaagcattgg aatgtataga caagtctgag attgaccttg
      121 ccatattgga catcatgctt cccggcacaa gcggccttac tatctgtcaa aaaataaggg
      181 acaagcacac ctatccgatt atcatgctga ccgggaaaga tacagaggta gataaaatta
      241 cagggttaac aatcggcgcg gatgattata taacgaagcc ctttcgccca ctggagttaa
      301 ttgctcgggt aaaggcccag ttgcgccgat acaaaaaatt cagtggagta aaggagcaga
      361 acgaaaatgt tatcgtccac tccggccttg tcattaatgt taacacccat gagtgttatc
      421 tgaacgagaa gcagttatcc cttactccca ccgagttttc aatactgcga atcctctgtg
      481 aaaacaaggg gaatgtggtt agctccgagc tgctatttca tgagatatgg ggcgacgaat
      541 atttcagcaa gagcaacaac accatcaccg tgcatatccg gcatttgcgc gaaaaaatga
      601 acgacaccat tgataatccg aaatatataa aaacggtatg gggggttggt tataaaattg
      661 aaaaataaaa aaaacgacta ttccaaacta gaacgaaaac tttacatgta tatcgttgca
      721 attgttgtgg tagcaattgt attcgtgttg tatattcgtt caatgatccg agggaaactt
      781 ggggattgga tcttaagtat tttggaaaac aaatatgact taaatcacct ggacgcgatg
      841 aaattatatc aatattccat acggaacaat atagatatct ttatttatgt ggcgattgtc
      901 attagtattc ttattctatg tcgcgtcatg ctttcaaaat tcgcaaaata ctttgacgag
      961 ataaataccg gcattgatgt acttattcag aacgaagata aacaaattga gctttctgcg
     1021 gaaatggatg ttatggaaca aaagctcaac acattaaaac ggactctgga aaagcgagag
     1081 caggatgcaa agctggccga acaaagaaaa aatgacgttg ttatgtactt ggcgcacgat
     1141 attaaaacgc cccttacatc cattatcggt tatttgagcc tgcttgacga ggctccagac
     1201 atgccggtag atcaaaaggc aaagtatgtg catatcacgt tggacaaagc gtatcgactc
     1261 gaacagctaa tcgacgagtt ttttgagatt acacggtata acctacaaac gataacgcta
     1321 acaaaaacgc acatagacct atactatatg ctggtgcaga tgaccgatga attttatcct
     1381 cagctttccg cacatggaaa acaggcggtt attcacgccc ccgaggatct gaccgtgtcc
     1441 ggcgaccctg ataaactcgc gagagtcttt aacaacattt tgaaaaacgc cgctgcatac
     1501 agtgaggata acagcatcat tgacattacc gcgggcctct ccggggatgt ggtgtcaatc
     1561 gaattcaaga acactggaag catcccaaaa gataagctag ctgccatatt tgaaaagttc
     1621 tataggctgg acaatgctcg ttcttccgat acgggtggcg cgggacttgg attggcgatt
     1681 gcaaaagaaa ttattgttca gcatggaggg cagatttacg cggaaagcaa tgataactat
     1741 acgacgttta gggtagagct tccagcgatg ccagacttgg ttgataaaag gaggtcctaa
     1801 gagatgtata taattttggc tctttgtcaa atgatgttgg taaagagaaa tct
//
LOCUS       DACEXQ010000474         1846 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.guided.323, whole genome shotgun sequence.
ACCESSION   DACEXQ010000474 DACEXQ010000000
VERSION     DACEXQ010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.guided.323"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            251..1162
                     /locus_tag="IXH04_002985"
     CDS             251..1162
                     /locus_tag="IXH04_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HAQ1853491.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            1315..1800
                     /gene="vanZ"
                     /locus_tag="IXH04_002986"
     CDS             1315..1800
                     /gene="vanZ"
                     /locus_tag="IXH04_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HAQ1853492.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
BASE COUNT          647 a          261 c          352 g          586 t
ORIGIN      
        1 gatcgcatag aggggtggta tttcacaccg cccattgtca acaggcagtt cagcctcgtt
       61 aaattcagca tgggtatcac ttatgaaaat tcatctacat tggtgataat agtaaatcca
      121 gtagggcgaa ataattgact gtaatttacg gggcaaaacg gcacaatctc aaacgagatt
      181 gtgccgttta aggggaagat tctagaaata tttcatactt ccaactatat agttaaggag
      241 gagactgaaa atgaagaagt tgtttttttt attgttattg ttattcttaa tatacttagg
      301 ttatgactac gttaatgaag cactgttttc tcaggaaaaa gtcgaatttc aaaattatga
      361 tcaaaatccc aaagaacatt tagaaaatag tgggacttct gaaaataccc aagagaaaac
      421 aattacagaa gaacaggttt atcaaggaaa tctgctatta atcaatagta aatatcctgt
      481 tcgccaagaa agtgtgaagt cagatatcgt gaatttatct aaacatgacg aattaataaa
      541 tggatacggg ttgcttgata gtaatattta tatgtcaaaa gaaatagcac aaaaattttc
      601 agagatggtc aatgatgctg taaagggtgg cgttagtcat tttattatta atagtggcta
      661 tcgagacttt gatgagcaaa gtgtgcttta ccaagaaatg ggggctgagt atgccttacc
      721 agcaggttat agtgagcata attcaggttt atcactagat gtaggatcaa gcttgacgaa
      781 aatggaacga gcccctgaag gaaagtggat agaagaaaat gcttggaaat acgggttcat
      841 tttacgttat ccagaggaca aaacagagtt aacaggaatt caatatgaac catggcatat
      901 tcgctatgtt ggtttaccac atagtgcgat tatgaaagaa aagaatttcg ttctcgagga
      961 atatatggat tacctaaaag aagaaaaaac catttctgtt agtgtaaatg gggaaaaata
     1021 tgagatcttt tattatcctg ttactaaaaa taccaccatt catgtgccga ctaatcttcg
     1081 ttatgagata tcaggaaaca atatagacgg tgtaattgtg acagtgtttc ccggatcaac
     1141 acatactaat tcaaggaggt aaggatggcg gaatgaaacc aacgaaatta atgaacagca
     1201 ttattgtact agcacttttg gggtaacgtt agctttttaa tttaaaaccc acgttaacta
     1261 ggacattgct atactaatga tacaacttaa acaaaagaat tagaggaaat tatattggga
     1321 aaaatattat ctagaggatt gctagcttta tatttagtga cactaatctg gttagtgtta
     1381 ttcaaattac aatacaatat tttatcagta tttaattatc atcaaagaag tcttaacttg
     1441 actccattta ctgctactgg gaatttcaga gagatgatag ataatgttat aatctttatt
     1501 ccatttggct tgcttttgaa tgtcaatttt aaagaaatcg gatttttacc taagtttgct
     1561 tttgtactgg ttttaagtct tacttttgaa ataattcaat ttatcttcgc tattggagcg
     1621 acagacataa cagatgtaat tacaaatact gttggaggct ttcttggact gaaattatat
     1681 ggtttaagca ataagcatat gaatcaaaaa aaattagaca gagttattat ttttgtaggt
     1741 atacttttgc tcgtattatt gctcgtttac cgtacccatt taagaataaa ttacgtgtaa
     1801 gatgtctaaa tcaagcaatc tgatctttca tacacataaa gatatt
//
LOCUS       DACEXQ010000475         1791 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.guided.325, whole genome shotgun sequence.
ACCESSION   DACEXQ010000475 DACEXQ010000000
VERSION     DACEXQ010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.guided.325"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            36..1514
                     /gene="msr"
                     /locus_tag="IXH04_002987"
     CDS             36..1514
                     /gene="msr"
                     /locus_tag="IXH04_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ1853493.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            1561..>1791
                     /locus_tag="IXH04_002988"
     CDS             1561..>1791
                     /locus_tag="IXH04_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAQ1853494.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKV"
BASE COUNT          619 a          303 c          385 g          484 t
ORIGIN      
        1 attgttgttt tacatgaaat aggagtgtaa aacatatgga aaatttagca gtaaatataa
       61 caaatctaca agtcagcttt ggcaaccagc tagaactatc tattgattct cttcgtgtct
      121 atcagcaaga tcgtataggg atcataggag aaaatggtgt cggtaaatcg actttgctca
      181 aactaatagc cggtgaactt tttcccgatc atgggaaaat ccaaacagag atcaccttca
      241 actacctgcc tcaattaacc tatctcgctg aagcaaagga cctaaatttg gaattagcca
      301 gtcatttcca gttaagactg gaagaaactt cagagcggaa atggagcgga ggggaagaac
      361 gaaagatcga gttgatacgt cttctttctt cttatgaaca agggatgctt ctagacgagc
      421 caacaaccca tctagataga aaaagtattg atcgactgat tgaagagctt cgttattatt
      481 atggtacgct ggtttttgtt agtcatgatc gctattttct agatgagcta gcatcgaaaa
      541 tctgggaagt aaaagacgga gaaatccgag agttttcggg gaattatagt gcctatctca
      601 ctcaaaagga attggagaaa aagactcagc tacgagaagc agagtcgatc atgaaagaga
      661 aaaaacgatt ggaaaaatcg atccaagaaa agaaaaaaca agcggaaaag ttggaaaaag
      721 tgtccagtaa aaagaaaaag caacaaatca gaccggatcg gttgtcttcc tctaaacaaa
      781 aagacagtgt acaaaaagcc atccaaaaga atgcgaaaac attagagaga agactccaaa
      841 aaataggaga aacaaccaag ccgcagcaga tgaaacaaat ccgttttcca gtaccaaaat
      901 ctcttgaact ccacagccgc tatcccatca tgggacaaaa tgtccaattg gaaagaagtg
      961 ggagaacatt actggtaaat ggtgattttc agttttcttt aggtaaaaaa atcgcgattg
     1021 tcggtgaaaa tggttcaggt aagacaacct tattagaaca tatccgcaaa caaggagaag
     1081 gaatccttct ctctccgaaa gtaagctttc aagtatatca gcaaaagggt tatcaaatga
     1141 catctgaaga atccatcatt cgttttgtca tgagacaaac agagttttca gaatcgcttg
     1201 tccgtagttt gctgaatcac ttagggtttg ctcaggaaac tttgacgaaa ccgttatgta
     1261 cattaagtgg gggagaagcg acccgtctga cgattgcttt gctttttact aagccaagta
     1321 atgtgttgct gttagatgaa ccgactaatt ttattgatat ggcaacgatc gaagctttag
     1381 agaagctaat gcaaatatat ccgggaacga ttttgtttac ttctcatgat tcttactttg
     1441 tcgagcgtac ggctgatgaa gtttatgaaa taaaagggca gaaaataaaa aaagtactta
     1501 cgagaaattt ttaaatgtgc tacgatgatt ttcgtgacaa atttaaagga gtggtacaga
     1561 ttgcctacaa ataaaaaaga aggaataatt tttacaacaa tgatgtgttt tttgatggtc
     1621 ttggggatga gcgcgtataa tttatggctg catcaagatc ttcatttatc aagtttgttg
     1681 attggtctag tgcctggatt cgtggtggct ttcattttgg atgtcttcat catcggtgtg
     1741 ctggcgaaaa aaattgcctt taaactacca gtaaatccag aaagcaaggt c
//
LOCUS       DACEXQ010000476         1725 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.guided.331, whole genome shotgun sequence.
ACCESSION   DACEXQ010000476 DACEXQ010000000
VERSION     DACEXQ010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.guided.331"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            46..1419
                     /gene="tet(L)"
                     /locus_tag="IXH04_002989"
     CDS             46..1419
                     /gene="tet(L)"
                     /locus_tag="IXH04_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAQ1853495.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
BASE COUNT          499 a          255 c          344 g          627 t
ORIGIN      
        1 ttttttaata aagatttgaa ttaaagtgta aaggaggaat taattatgaa gtgtaatgaa
       61 tgtaacaggg ttaaatacaa ccaaatttta atttggcttt gcgttttatc tttttttagc
      121 gtattaaatg aaatggtttt gaacgtctca ttacctgata ttgcaaatga ttttaataaa
      181 ccacctgcga gtacaaactg ggtgaacaca gcctttatgt taaccttttc cattggaaca
      241 gctgtatatg gaaagctatc tgatcaatta ggcatcaaaa ggttactcct atttggaatt
      301 ataataaatt gtttcgggtc ggtaattggg tttgttggcc attctttctt ttccttactt
      361 attatggctc gttttattca aggggctggt gcagctgcat ttccagcact cgtaatggtt
      421 gtagttgcgc gctatattcc aaaggaaaat aggggtaaag catttggtct tattggatcg
      481 atagtagcca tgggagaagg agtcggtcca gcgattggtg gaatgatagc ccattatatt
      541 cattggtcct atcttctact cattcctatg ataacaatta tcactgttcc gtttcttatg
      601 aaattattaa agaaagaagt aaggataaaa ggtcattttg atatcaaagg aattatacta
      661 atgtctgtag gcattgtatt ttttatgttg tttacaacat catatagcat ttcttttctt
      721 atcgttagcg tgctgtcatt cctgatattt gtaaaacata tcaggaaagt aacagatcct
      781 tttgttgatc ccggattagg gaaaaatata ccttttatga ttggagttct ttgtggggga
      841 attatatttg gaacagtagc agggtttgtc tctatggttc cttatatgat gaaagatgtt
      901 caccagctaa gtactgccga aatcggaagt gtaattattt tccctggaac aatgagtgtc
      961 attattttcg gctacattgg tgggatactt gttgatagaa gaggtccttt atacgtgtta
     1021 aacatcggag ttacatttct ttctgttagc tttttaactg cttcctttct tttagaaaca
     1081 acatcatggt tcatgacaat tataatcgta tttgttttag gtgggctttc gttcaccaaa
     1141 acagttatat caacaattgt ttcaagtagc ttgaaacagc aggaagctgg tgctggaatg
     1201 agtttgctta actttaccag ctttttatca gagggaacag gtattgcaat tgtaggtggt
     1261 ttattatcca tacccttact tgatcaaagg ttgttaccta tggaagttga tcagtcaact
     1321 tatctgtata gtaatttgtt attacttttt tcaggaatca ttgtcattag ttggctggtt
     1381 accttgaatg tatataaaca ttctcaaagg gatttctaaa tcgttaaggg atcaactttg
     1441 ggagagagtt caaaattgat ccttttttta taacaggaat tcaaatcttt ttgttccatt
     1501 aaagggcgcg attgctgaat aaaagatacg agagacctct cttgtatctt ttttattttg
     1561 agtggttttg tccgttacac tagaaaaccg aaagacaata aaaattttat tcttgctgag
     1621 tctggctttc ggtaagctag acaaaacgga caaaataaaa attggcaagg gtttaaaggt
     1681 ggagattttt tgagtgatct tctcaaaaaa tactacctgt ccctt
//
LOCUS       DACEXQ010000477         1674 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.guided.334, whole genome shotgun sequence.
ACCESSION   DACEXQ010000477 DACEXQ010000000
VERSION     DACEXQ010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.guided.334"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            138..680
                     /gene="sat4"
                     /locus_tag="IXH04_002990"
     CDS             138..680
                     /gene="sat4"
                     /locus_tag="IXH04_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAQ1853496.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            773..1567
                     /locus_tag="IXH04_002991"
                     /note="aph(3')-IIIa"
     CDS             773..1567
                     /locus_tag="IXH04_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAQ1853497.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          558 a          282 c          400 g          434 t
ORIGIN      
        1 gaaaggcttc attatgccta tccggagtat gataggaata taacaaaata taccagggac
       61 atgtataaaa aatacactgg taaaaccggc tgcctggata gcacatatgc cgctgatata
      121 gaagagaggc gggaacagtg attacagaaa tgaaagcagg gcacctgaaa gatatcgata
      181 aacccagcga accatttgag gtgataggta agattatacc gaggtatgaa aacgagaatt
      241 ggacctttac agaattactc tatgaagcgc catatttaaa aagctaccaa gacgaagagg
      301 atgaagagga tgaggaggca gattgccttg aatatattga caatactgat aagataatat
      361 atctttacta ccaagacgat aaatgcgtcg gaaaagttaa actgcgaaaa aattggaacc
      421 ggtacgctta tatagaagat atcgccgtat gtaaggattt cagggggcaa ggcataggca
      481 gcgcgcttat caatatatct atagaatggg caaagcataa aaacttgcat ggactaatgc
      541 ttgaaaccca ggacaataac cttatagctt gtaaattcta tcataattgt ggtttcaaaa
      601 tcggctccgt cgatactatg ttatacgcca actttgaaaa caactttgaa aaagctgttt
      661 tctggtattt aaggttttag aatgcaagga acagtgaatt ggagttcgtc ttgttataat
      721 tagcttcttg gggtatcttt aaatactgta gaaaagagga aggaaataat aaatggctaa
      781 aatgagaata tcaccggaat tgaaaaaact gatcgaaaaa taccgctgcg taaaagatac
      841 ggaaggaatg tctcctgcta aggtatataa gctggtggga gaaaatgaaa acctatattt
      901 aaaaatgacg gacagccggt ataaagggac cacctatgat gtggaacggg aaaaggacat
      961 gatgctatgg ctggaaggaa agctgcctgt tccaaaggtc ctgcactttg aacggcatga
     1021 tggctggagc aatctgctca tgagtgaggc cgatggcgtc ctttgctcgg aagagtatga
     1081 agatgaacaa agccctgaaa agattatcga gctgtatgcg gagtgcatca ggctctttca
     1141 ctccatcgac atatcggatt gtccctatac gaatagctta gacagccgct tagccgaatt
     1201 ggattactta ctgaataacg atctggccga tgtggattgc gaaaactggg aagaagacac
     1261 tccatttaaa gatccgcgcg agctgtatga ttttttaaag acggaaaagc ccgaagagga
     1321 acttgtcttt tcccacggcg acctgggaga cagcaacatc tttgtgaaag atggcaaagt
     1381 aagtggcttt attgatcttg ggagaagcgg cagggcggac aagtggtatg acattgcctt
     1441 ctgcgtccgg tcgatcaggg aggatatcgg ggaagaacag tatgtcgagc tattttttga
     1501 cttactgggg atcaagcctg attgggagaa aataaaatat tatattttac tggatgaatt
     1561 gttttagtac ctagatttag atgtctaaaa agctttaact acaagctttt tagacatcta
     1621 atcttttctg aagtacatcc gcaactgtcc atactctgat gttttatatc tttt
//
LOCUS       DACEXQ010000478         1392 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.guided.354, whole genome shotgun sequence.
ACCESSION   DACEXQ010000478 DACEXQ010000000
VERSION     DACEXQ010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.guided.354"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            295..768
                     /gene="dfrF"
                     /locus_tag="IXH04_002992"
     CDS             295..768
                     /gene="dfrF"
                     /locus_tag="IXH04_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HAQ1853498.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            895..>1392
                     /locus_tag="IXH04_002993"
     CDS             895..>1392
                     /locus_tag="IXH04_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAQ1853499.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYL"
BASE COUNT          520 a          160 c          287 g          425 t
ORIGIN      
        1 acagcagact atttcatttc tgcttgatta ccttaaaact tattagtcta tggtttgtca
       61 agggcttgtt gcgtgagaaa tgctgaatat caccctttac aaaacatagg ctgataagta
      121 acttttcaaa agcagaaaag aaatggcaat gcttataaca atatgatata atgcagatga
      181 agacaaaaaa ggaatttgtc gagctggtta gttcgagata attttgagtt tgttaagatg
      241 tggtgcactt gatggaatca caggtacgac atcatattat ggaggatgaa atagatgata
      301 ggtttgattg ttgcgaggtc aaagaataat gttataggca agaatggtaa tataccatgg
      361 aaaataaagg gagaacaaaa gcaatttaga gagttaacaa cgggtaatgt ggttattatg
      421 gggcgaaagt cttatgaaga aatcggtcat ccgttgccta atagaatgaa tattgttgtt
      481 tccaccacaa cagagtatca aggagataat ttagtttcag ttaaatcatt agaagatgca
      541 ttattattgg ctaaaggacg agatgtatac atatctggtg gatatggact atttaaggaa
      601 gctttgcaaa tagtagataa aatgtatatc acagaagtag atttaaatat tgaagatgga
      661 gatacattct ttccagaatt tgatatcaat gattttgaag ttttgatagg ggaaacactt
      721 ggtgaggaag tgaaatatac gagaacattt tatgtaagga aaaaatgaat tgagtaaatt
      781 ttggatttag ggtgttttca taaatatatt gctttttatg catatataaa ttgttgtaag
      841 acaaattcca gtttgtatgg ggcgataaaa tcagaatttg agagaggatt gaatatgaat
      901 aaggaatggt ctgaacttaa taaaacaatg caggcacaaa taaaaaagaa agatacttat
      961 aagaggggta ttgatacttt acttacttta cgaagtcagt taatacaaac cttagtatct
     1021 ttcaaagaag aattatgcag agaagatttt aactcaatcc ccttcataaa cgctgatggt
     1081 tatcatagta agacgattgc ttattctatt tggcatattt tccgcattga agatatagtt
     1141 gtgcatacag tgataaatga agatgaacaa gtattttttg caggcaatta tcaagagcgt
     1201 atcaattcac ctatcattac aacaggaaat gaactcatga aacagcagat tgcagacttt
     1261 tcaaaacagc ttaatctgga agaattatat ttatatattt tcgaagtatg ggaaagcact
     1321 gagaaaatgc ttgaacggtt atcttatgat gaattgaaaa gaaaaatacc gaaagaaaga
     1381 aaaggatact ta
//
LOCUS       DACEXQ010000479         1281 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.guided.364, whole genome shotgun sequence.
ACCESSION   DACEXQ010000479 DACEXQ010000000
VERSION     DACEXQ010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.guided.364"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..249
                     /locus_tag="IXH04_002994"
     CDS             <1..249
                     /locus_tag="IXH04_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAQ1853500.1"
                     /translation="VKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQFLLKLQRM
                     PNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            242..1273
                     /gene="vanA"
                     /locus_tag="IXH04_002995"
     CDS             242..1273
                     /gene="vanA"
                     /locus_tag="IXH04_002995"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAQ1853501.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
BASE COUNT          389 a          242 c          320 g          330 t
ORIGIN      
        1 gttaaagcat tagaaaacgg gaaactgggc ggtgccgcat tggatgtatt ggaaggagag
       61 gaagagtttt tctactctga ttgcacccaa aaaccaattg ataatcaatt tttacttaaa
      121 cttcaaagaa tgcctaacgt gataatcaca ccgcatacgg cctattatac cgagcaagcg
      181 ttgcgtgata ccgttgaaaa aaccattaaa aactgtttgg attttgaaag gagacaggag
      241 catgaataga ataaaagttg caatactgtt tgggggttgc tcagaggagc atgacgtatc
      301 ggtaaaatct gcaatagaga tagccgctaa cattaataaa gaaaaatacg agccgttata
      361 cattggaatt acgaaatctg gtgtatggaa aatgtgcgaa aaaccttgcg cggaatggga
      421 aaacgacaat tgctattcag ctgtactctc gccggataaa aaaatgcacg gattacttgt
      481 taaaaagaac catgaatatg aaatcaacca tgttgatgta gcattttcag ctttgcatgg
      541 caagtcaggt gaagatggat ccatacaagg tctgtttgaa ttgtccggta tcccttttgt
      601 aggctgcgat attcaaagct cagcaatttg tatggacaaa tcgttgacat acatcgttgc
      661 gaaaaatgct gggatagcta ctcccgcctt ttgggttatt aataaagatg ataggccggt
      721 ggcagctacg tttacctatc ctgtttttgt taagccggcg cgttcaggct catccttcgg
      781 tgtgaaaaaa gtcaatagcg cggacgaatt ggactacgca attgaatcgg caagacaata
      841 tgacagcaaa atcttaattg agcaggctgt ttcgggctgt gaggtcggtt gtgcggtatt
      901 gggaaacagt gccgcgttag ctgttggcga ggtggaccaa atcaggctgc agtacggaat
      961 ctttcgtatt catcaggaag tcgagccgga aaaaggctct gaaaacgcag ttataaccgt
     1021 tcccgcagac ctttcagcag aggagcgagg acggatacag gaaacggcaa aaaaaatata
     1081 taaagcgctc ggctgtagag gtctagcccg tgtggatatg tttttacaag ataacggccg
     1141 cattgtactg aacgaagtca atactctgcc cggtttcacg tcatacagtc gttatccccg
     1201 tatgatggcc gctgcaggta ttgcacttcc cgaactgatt gaccgcttga tcgtattagc
     1261 gttaaagggg tgataagcat g
//
LOCUS       DACEXQ010000480         1274 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.guided.365, whole genome shotgun sequence.
ACCESSION   DACEXQ010000480 DACEXQ010000000
VERSION     DACEXQ010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.guided.365"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            246..1214
                     /locus_tag="IXH04_002996"
     CDS             246..1214
                     /locus_tag="IXH04_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAQ1853502.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1207..>1274
                     /locus_tag="IXH04_002997"
     CDS             1207..>1274
                     /locus_tag="IXH04_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine-D-lactate ligase"
                     /protein_id="HAQ1853503.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKS"
BASE COUNT          379 a          243 c          302 g          350 t
ORIGIN      
        1 gatgctggtg tcatccacca acatcaaaaa gtatagagcc ataatttttt aggaaaatct
       61 caaggttatc tttacttttt cttaggaaat taacaattta atattaagaa acggctcgtt
      121 cttacacggt agacttaata ccgtaagaac gagccgtttt cgttcttcag agaaagattt
      181 gacaagatta ccattggcat ccccgtttta tttggtgcct ttcacagaaa gggttggtct
      241 taattatgaa taacatcggc attactgttt atggatgtga gcaggatgag gcagatgcat
      301 tccatgctct ttcgcctcgc tttggcgtta tggcaacgat aattaacgcc aacgtgtcgg
      361 aatccaacgc caaatccgcg cctttcaatc aatgtatcag tgtgggacat aaatcagaga
      421 tttccgcctc tattcttctt gcgctgaaga gagccggtgt gaaatatatt tctacccgaa
      481 gcatcggctg caatcatata gatacaactg ctgctaagag aatgggcatc actgtcgaca
      541 atgtggcgta ctcgccggat agcgttgccg attatactat gatgctaatt cttatggcag
      601 tacgcaacgt aaaatcgatt gtgcgctctg tggaaaaaca tgatttcagg ttggacagcg
      661 accgtggcaa ggtactcagc gacatgacag ttggtgtggt gggaacgggc cagataggca
      721 aagcggttat tgagcggctg cgaggatttg gatgtaaagt gttggcttat agtcgcagcc
      781 gaagtataga ggtaaactat gtaccgtttg atgagttgct gcaaaatagc gatatcgtta
      841 cgcttcatgt gccgctcaat acggatacgc actatattat cagccacgaa caaatacaga
      901 gaatgaagca aggagcattt cttatcaata ctgggcgcgg tccacttgta gatacctatg
      961 agttggttaa agcattagaa aacgggaaac tgggcggtgc cgcattggat gtattggaag
     1021 gagaggaaga gtttttctac tctgattgca cccaaaaacc aattgataat caatttttac
     1081 ttaaacttca aagaatgcct aacgtgataa tcacaccgca tacggcctat tataccgagc
     1141 aagcgttgcg tgataccgtt gaaaaaacca ttaaaaactg tttggatttt gaaaggagac
     1201 aggagcatga atagaataaa agttgcaata ctgtttgggg gttgctcaga ggagcatgac
     1261 gtatcggtaa aatc
//
LOCUS       DACEXQ010000481         1244 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.guided.369, whole genome shotgun sequence.
ACCESSION   DACEXQ010000481 DACEXQ010000000
VERSION     DACEXQ010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1244
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.guided.369"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            529..1077
                     /gene="aac(6')"
                     /locus_tag="IXH04_002998"
     CDS             529..1077
                     /gene="aac(6')"
                     /locus_tag="IXH04_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAQ1853504.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          444 a          180 c          252 g          368 t
ORIGIN      
        1 aaacgggtta aaattttaaa gagtgatcga tctgactgcg gctgtagatg gagtagaggt
       61 gaacctaaga caggataagt gatgaataag gtggcatgaa caccttgaaa tcatcaataa
      121 gtatcctaaa aacgaacaag tacaaaaaca ttgaaaacga caataatgaa tttttgttcc
      181 tcttagtatt tctaccgatt ccctttgctt ttttgtaatt atctaaaaat tctaaaaatt
      241 aagattatta tatgaaaaca ggttttattt gtcaattcat aattggtttt ttttaaagca
      301 aaagataatc tatatcatga tgaacaaaca gagttttttc ctccaattga tggctttcct
      361 gatctttcct attttgaata aagtgtttct gtataatttt tttattttat aagtaagata
      421 aataatgaaa gaatagagat actgattggt agctaaagat ttttgagcga gaacatttcg
      481 ttttttcaaa aggactgtta gaataagcaa gaacaagggg gaagacgtat gataatcagt
      541 gaatttgacc gtaataatcc agtattgaaa gatcagcttt ctgatttact gagactgact
      601 tggccggaag aatatggaga cagctcggca gaagaagtag aagaaatgat gaatccagaa
      661 cgaatcgcgg tagcagcggt agaccaagat gagttagtag gatttattgg tgcaatccct
      721 caatacggta tcacaggttg ggaattgcat ccattagttg tagaaagctc ccgacgaaag
      781 aaccaaatag gtactcgatt agtcaattac ttagaaaaag aagtagcttc cagaggagga
      841 atcacgattt atttaggtac ggatgattta gaccatggaa caacgttaag tcaaacggac
      901 ctgtatgagc atacatttga taaagtggct tccatccaga accttcgtga acatccgtat
      961 gaattctatg aaaaattagg ttataaaatc gtaggtgtct taccaaatgc aaatggctgg
     1021 gacaaaccgg atatttggat ggcaaaaacg attattcctc gaccaaattc tcaataataa
     1081 aaagaggttg tgacagaagt aatcagcttc aagaaataag aaggaattta cgaaaattac
     1141 ttcttgaatt tttgtgaaat tccaacttat ttccgaagaa gctacttttg gaacaccgtg
     1201 taccagaggt tgtgaaaaag tcgcttagtt tcgagtagta agaa
//
LOCUS       DACEXQ010000482         1139 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.guided.385, whole genome shotgun sequence.
ACCESSION   DACEXQ010000482 DACEXQ010000000
VERSION     DACEXQ010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.guided.385"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            14..709
                     /gene="vanR"
                     /locus_tag="IXH04_002999"
     CDS             14..709
                     /gene="vanR"
                     /locus_tag="IXH04_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAQ1853505.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            687..>1139
                     /locus_tag="IXH04_003000"
     CDS             687..>1139
                     /locus_tag="IXH04_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAQ1853506.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLA"
BASE COUNT          388 a          205 c          241 g          305 t
ORIGIN      
        1 aagggtgata actatgagcg ataaaatact tattgtggat gatgaacatg aaattgccga
       61 tttggttgaa ttatacttaa aaaacgagaa ttatacggtt ttcaaatact ataccgccaa
      121 agaagcattg gaatgtatag acaagtctga gattgacctt gccatattgg acatcatgct
      181 tcccggcaca agcggcctta ctatctgtca aaaaataagg gacaagcaca cctatccgat
      241 tatcatgctg accgggaaag atacagaggt agataaaatt acagggttaa caatcggcgc
      301 ggatgattat ataacgaagc cctttcgccc actggagtta attgctcggg taaaggccca
      361 gttgcgccga tacaaaaaat tcagtggagt aaaggagcag aacgaaaatg ttatcgtcca
      421 ctccggcctt gtcattaatg ttaacaccca tgagtgttat ctgaacgaga agcagttatc
      481 ccttactccc accgagtttt caatactgcg aatcctctgt gaaaacaagg ggaatgtggt
      541 tagctccgag ctgctatttc atgagatatg gggcgacgaa tatttcagca agagcaacaa
      601 caccatcacc gtgcatatcc ggcatttgcg cgaaaaaatg aacgacacca ttgataatcc
      661 gaaatatata aaaacggtat ggggggttgg ttataaaatt gaaaaataaa aaaaacgact
      721 attccaaact agaacgaaaa ctttacatgt atatcgttgc aattgttgtg gtagcaattg
      781 tattcgtgtt gtatattcgt tcaatgatcc gagggaaact tggggattgg atcttaagta
      841 ttttggaaaa caaatatgac ttaaatcacc tggacgcgat gaaattatat caatattcca
      901 tacggaacaa tatagatatc tttatttatg tggcgattgt cattagtatt cttattctat
      961 gtcgcgtcat gctttcaaaa ttcgcaaaat actttgacga gataaatacc ggcattgatg
     1021 tacttattca gaacgaagat aaacaaattg agctttctgc ggaaatggat gttatggaac
     1081 aaaagctcaa cacattaaaa cggactctgg aaaagcgaga gcaggatgca aagctggcc
//
LOCUS       DACEXQ010000483         1121 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.guided.387, whole genome shotgun sequence.
ACCESSION   DACEXQ010000483 DACEXQ010000000
VERSION     DACEXQ010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.guided.387"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            complement(<1..164)
                     /locus_tag="IXH04_003001"
     CDS             complement(<1..164)
                     /locus_tag="IXH04_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ1853507.1"
                     /translation="MKYILSFCTPKLNYLLAYIRLYSGVLHLRDSVRVSEKEKIKVTE
                     MYTSINGELC"
     gene            394..891
                     /gene="dfrG"
                     /locus_tag="IXH04_003002"
     CDS             394..891
                     /gene="dfrG"
                     /locus_tag="IXH04_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAQ1853508.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
BASE COUNT          412 a          134 c          205 g          370 t
ORIGIN      
        1 ttacataatt caccatttat tgaagtatac atttctgtaa cttttatttt ttctttttct
       61 gatactctaa ccgaatctcg taaatgtagt actccactat aaaggcgtat atatgcaagt
      121 aagtagttca gttttggagt acaaaatgac aaaatgtact tcaaaatgga ctactttttt
      181 cattttaact tacataccaa attacataca aaatgattta taaaatgcaa ttgaaaatga
      241 taattgaatg aaaatttgtg attttgtggt attatgataa tataacttat agaaaaaatt
      301 gcttattcaa ctaacgggca ggataatgag aaaaggtttt ttgatagaaa taagaaatta
      361 gatggtaaat tagttaaaaa ggctgtgtta taaatgaaag tttctttgat tgctgcgatg
      421 gataagaata gagtgattgg caaagagaat gacattcctt ggaggattcc caaggactgg
      481 gaatatgtta aaaatactac aaagggacat ccgataatat taggtaggaa gaaccttgaa
      541 tcaatcggaa gagccttacc tgacagaaga aatattattc tgacgagaga taaggggttt
      601 acctttaatg gttgtgaaat tgttcattca atagaagatg tttttgagtt atgtaaaaac
      661 gaagaagaaa tttttatttt cggaggagaa cagatttata atttgttttt cccttatgtt
      721 gagaaaatgt acatcacaaa aatacatcat gaattcgaag gagatacttt ttttccagaa
      781 gtgaattatg aggaatggaa tgaggtattt gcccaaaaag ggataaagaa tgataaaaat
      841 ccgtataact actattttca tgtatatgaa agaaaaaact tattgagtta acgggtgcgt
      901 taattgaaga agaacagtaa agataaagca caagagcttg ggattactcc aagctctttt
      961 tgtcatttga aataaattat atctttccaa tcaaattgtt tatctgtaat ccctaatctt
     1021 tgagcaggtg tcaaaacctt tttatccgct gatttaaaag ggcgacaaaa gttgtagtaa
     1081 gttcttagaa ttgtaatcgc aaattgagca tacttcggat t
//
LOCUS       DACEXQ010000484         1071 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.guided.390, whole genome shotgun sequence.
ACCESSION   DACEXQ010000484 DACEXQ010000000
VERSION     DACEXQ010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.guided.390"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            109..192
                     /locus_tag="IXH04_003003"
     CDS             109..192
                     /locus_tag="IXH04_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAQ1853509.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            317..1054
                     /gene="erm(B)"
                     /locus_tag="IXH04_003004"
     CDS             317..1054
                     /gene="erm(B)"
                     /locus_tag="IXH04_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAQ1853510.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          403 a          172 c          166 g          330 t
ORIGIN      
        1 ttatttgata gtagatttaa aactttgcaa cagaaccatt ttgtgtataa taggaattga
       61 agttaaatta gatgctaaaa atttgtaatt aagaaggagg gattcgtcat gttggtattc
      121 caaatgcgta atgtagataa aacatctact gttttgaaac agactaaaaa cagtgattac
      181 gcagataaat aaatacgtta gattaattcc taccagtgac taatcttatg actttttaaa
      241 cagataacta aaattacaaa caaatcgttt aacttctgta tttatttaca gatgtaataa
      301 cttcaggagt gattacatga acaaaaatat aaaatattct caaaactttt taacgagtga
      361 aaaagtactc aaccaaataa taaaacaatt gaatttaaaa gaaaccgata ccgtttacga
      421 aattggaaca ggtaaagggc atttaacgac gaaactggct aaaataagta aacaggtaac
      481 gtctattgaa ttagacagtc atctattcaa cttatcgtca gaaaaattaa aactgaacat
      541 tcgtgtcact ttaattcacc aagatattct acagtttcaa ttccctaaca aacagaggta
      601 taaaattgtt gggagtattc cttaccattt aagcacacaa attattaaaa aagtggtttt
      661 tgaaagccat gcgtctgaca tctatctgat tgttgaagaa ggattctaca agcgtacctt
      721 ggatattcac cgaacactag ggttgctctt gcacactcaa gtctcgattc agcaattgct
      781 taagctgcca gcggaatgct ttcatcctaa accaaaagta aacagtgtct taataaaact
      841 tacccgccat accacagatg ttccagataa atattggaag ctatatacgt actttgtttc
      901 aaaatgggtc aatcgagaat atcgtcaact gtttactaaa aatcagtttc atcaagcaat
      961 gaaacacgcc aaagtaaaca atttaagtac cgttacttat gagcaagtat tgtctatttt
     1021 taatagttat ctattattta acgggaggaa ataattctat gagtcgcttt t
//
LOCUS       DACEXQ010000485          794 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.guided.420, whole genome shotgun sequence.
ACCESSION   DACEXQ010000485 DACEXQ010000000
VERSION     DACEXQ010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..794
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.guided.420"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            38..646
                     /locus_tag="IXH04_003005"
     CDS             38..646
                     /locus_tag="IXH04_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAQ1853511.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          243 a          146 c          186 g          219 t
ORIGIN      
        1 accgcttgat cgtattagcg ttaaaggggt gataagcatg gaaataggat ttactttttt
       61 agatgaaata gtacacggtg ttcgttggga cgctaaatat gccacttggg ataatttcac
      121 cggaaaaccg gttgacggtt atgaagtaaa tcgcattgta gggacatacg agttggctga
      181 atcgcttttg aaggcaaaag aactggctgc tacccaaggg tacggattgc ttctatggga
      241 cggttaccgt cctaatcgtg ctgtaaactg ttttatgcaa tgggctgcac agccggaaaa
      301 taacctgaca aaggaaagtt attatcccaa tattgaccga actgagatga tttcaaaagg
      361 atacgtggct tcaaaatcaa gccatagccg cggcagtgcc attgatctta cgctttatcg
      421 attagacacg ggtgagcttg taccaatggg gagccgattt gattttatgg atgaacgctc
      481 tcatcatgcg gcaaatggaa tatcatgcaa tgaagcgcaa aatcgcagac gtttgcgctc
      541 catcatggaa aacagtgggt ttgaagcata tagcctcgaa tggtggcact atgtattaag
      601 agacgaacca taccccaata gctattttga tttccccgtt aaataaactt ttaaccgttg
      661 cacggacaaa ctatataagc taactctttc ggcaggaaac ccgacgtatg taactggttc
      721 ttagggaatt tatatatagt agatagtatt gaagaggttc tgttgcaaag ttttaaataa
      781 agaataaaat ccct
//
LOCUS       DACEXQ010000486          570 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.guided.444, whole genome shotgun sequence.
ACCESSION   DACEXQ010000486 DACEXQ010000000
VERSION     DACEXQ010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.guided.444"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..>570
                     /locus_tag="IXH04_003006"
     CDS             <1..>570
                     /locus_tag="IXH04_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAQ1853512.1"
                     /translation="DFILYLKLCNRTESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQI
                     YKMYDELYTDYHYPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAV
                     ILDPHKNNPRAIRAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKY
                     LIEHYFDNFKVDSIEIIGSGYDSVAYLVN"
BASE COUNT          227 a           53 c          104 g          186 t
ORIGIN      
        1 gggattttat tctttattta aaactttgca acagaaccga atcattaaaa aaacattata
       61 cagagccttg ggaagatgaa gtttttagag taattattga atataacaat gttcctattg
      121 gatatggaca aatatataaa atgtatgatg agttatatac tgattatcat tatccaaaaa
      181 ctgatgagat agtctatggt atggatcaat ttataggaga gccaaattat tggagtaaag
      241 gaattggtac aagatatatt aaattgattt ttgaattttt gaaaaaagaa agaaatgcta
      301 atgcagttat tttagaccct cataaaaata atccaagagc aataagggca taccaaaaat
      361 ctggttttag aattattgaa gatttgccag aacatgaatt acacgagggc aaaaaagaag
      421 attgttattt aatggaatat agatatgatg ataatgccac aaatgttaag gcaatgaaat
      481 atttaattga gcattacttt gataatttca aagtagatag tattgaaata atcggtagtg
      541 gttatgatag tgtggcatat ttagttaata
//
LOCUS       DACEXQ010000487          544 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.guided.447, whole genome shotgun sequence.
ACCESSION   DACEXQ010000487 DACEXQ010000000
VERSION     DACEXQ010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.guided.447"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            <1..>544
                     /locus_tag="IXH04_003007"
     CDS             <1..>544
                     /locus_tag="IXH04_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HAQ1853513.1"
                     /translation="FLFSIYTKYFTLSTNVKIPNIEYSYISDELSILGYKEIKGTFLT
                     PEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYILLRETIYN
                     DLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGI
                     IDEYCDFIYLLEDSEEEIGTN"
BASE COUNT          214 a           50 c           85 g          195 t
ORIGIN      
        1 cttccttttt tctatttaca caaaatattt tacactctca actaatgtaa aaattcctaa
       61 tattgaatat tcgtatatta gtgatgaatt atctatacta ggttataaag aaattaaagg
      121 aactttttta acaccagaaa tttattctac tatgtcagaa gaagaacaaa atttgttaaa
      181 acgagatatt gccagttttt taagacaaat gcacggttta gattatacag atattagtga
      241 atgtactatt gataataaac aaaatgtatt agaagagtat atattgttgc gtgaaactat
      301 ttataatgat ttaactgata tagaaaaaga ttatatagaa agttttatgg aaagactaaa
      361 tgcaacaaca gtttttgagg gtaaaaagtg tttatgccat aatgatttta gttgtaatca
      421 tctattgtta gatggcaata atagattaac tggaataatt gattttggag attctggaat
      481 tatagatgaa tattgtgatt ttatatactt acttgaagat agtgaagaag aaataggaac
      541 aaat
//
LOCUS       DACEXQ010000488          496 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain ST584 isolate 1929
            SAMN10039099-rid9586303.guided.459, whole genome shotgun sequence.
ACCESSION   DACEXQ010000488 DACEXQ010000000
VERSION     DACEXQ010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10039099
            Sequence Read Archive: SRR7839863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705239.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705239.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 10:57:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,867
            CDSs (with protein)               :: 2,867
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 30 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10039099-rid9586303.guided.459"
                     /strain="ST584"
                     /isolate="1929"
                     /isolation_source="Facility 4"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Southeast Michigan"
                     /collection_date="2014"
                     /collected_by="University of Michigan Medical School"
     gene            6..>496
                     /locus_tag="IXH04_003008"
     CDS             6..>496
                     /locus_tag="IXH04_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1853514.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNE"
BASE COUNT          154 a           87 c          118 g          137 t
ORIGIN      
        1 agaaaatgtt agctgcaatt tgggcacagg atgaaaacgg attgattgga aaagaagacc
       61 gtcttccttg gcgtttgcca aatgatctga aattcttcaa acaaatgaca gaagcaaata
      121 cactagtgat gggacgaaaa acattcgaag ggatgggtag ccgtccgttg ccgaatcgtc
      181 agacgattgt tttgacgaga gattcttcct ataaagcaga aggcgtgcat gtcatgcata
      241 gtgtggaaga cgtgcttgct ctggaaaaag aaacagacgg tatctttttc atcgctggag
      301 ggtcagctgt ttatcaagaa tttctgccat tttgtacgat attataccgt acagtcatcc
      361 atcatgtatt cgatggagat gcttattttc cacctgttga ctggtctgat tggtcactga
      421 tcaatactag tcaaggagaa atggatgaaa aaaatcgtta tccgcatcaa tttgaaacgt
      481 atcagcgtaa tgagaa
//
